LOCUS       DADLZM010000001        97907 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLZM010000001 DADLZM010000000
VERSION     DADLZM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 97907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.001"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            2..124
                     /locus_tag="JNY73_000001"
     ncRNA           2..124
                     /ncRNA_class="other"
                     /locus_tag="JNY73_000001"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            244..585
                     /locus_tag="JNY73_000002"
     CDS             244..585
                     /locus_tag="JNY73_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY8046156.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(576..1502)
                     /locus_tag="JNY73_000003"
     CDS             complement(576..1502)
                     /locus_tag="JNY73_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8046157.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1592..2590
                     /locus_tag="JNY73_000004"
     CDS             1592..2590
                     /locus_tag="JNY73_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY8046158.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2587..2805)
                     /locus_tag="JNY73_000005"
     CDS             complement(2587..2805)
                     /locus_tag="JNY73_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046159.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2807..4822)
                     /gene="ligA"
                     /locus_tag="JNY73_000006"
     CDS             complement(2807..4822)
                     /gene="ligA"
                     /locus_tag="JNY73_000006"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY8046160.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGSTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQELMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4893..5780)
                     /gene="zipA"
                     /locus_tag="JNY73_000007"
     CDS             complement(4893..5780)
                     /gene="zipA"
                     /locus_tag="JNY73_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY8046161.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMT
                     PQKLREYQDIIREVKDANA"
     gene            6010..6771
                     /gene="cysZ"
                     /locus_tag="JNY73_000008"
     CDS             6010..6771
                     /gene="cysZ"
                     /locus_tag="JNY73_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY8046162.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6956..7927
                     /gene="cysK"
                     /locus_tag="JNY73_000009"
     CDS             6956..7927
                     /gene="cysK"
                     /locus_tag="JNY73_000009"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY8046163.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8311..8568
                     /gene="ptsH"
                     /locus_tag="JNY73_000010"
     CDS             8311..8568
                     /gene="ptsH"
                     /locus_tag="JNY73_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY8046164.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8613..10340
                     /gene="ptsI"
                     /locus_tag="JNY73_000011"
     CDS             8613..10340
                     /gene="ptsI"
                     /locus_tag="JNY73_000011"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY8046165.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10381..10890
                     /gene="crr"
                     /locus_tag="JNY73_000012"
     CDS             10381..10890
                     /gene="crr"
                     /locus_tag="JNY73_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY8046166.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10932..11783)
                     /gene="pdxK"
                     /locus_tag="JNY73_000013"
                     /gene_synonym="thiJ"
     CDS             complement(10932..11783)
                     /gene="pdxK"
                     /locus_tag="JNY73_000013"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY8046167.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11888..12262
                     /locus_tag="JNY73_000014"
     CDS             11888..12262
                     /locus_tag="JNY73_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY8046168.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12295..13018
                     /locus_tag="JNY73_000015"
                     /pseudo
     CDS             12295..13018
                     /locus_tag="JNY73_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13222..14133)
                     /gene="cysM"
                     /locus_tag="JNY73_000016"
     CDS             complement(13222..14133)
                     /gene="cysM"
                     /locus_tag="JNY73_000016"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY8046169.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14267..15364)
                     /gene="cysA"
                     /locus_tag="JNY73_000017"
     CDS             complement(14267..15364)
                     /gene="cysA"
                     /locus_tag="JNY73_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY8046170.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15354..16229)
                     /gene="cysW"
                     /locus_tag="JNY73_000018"
     CDS             complement(15354..16229)
                     /gene="cysW"
                     /locus_tag="JNY73_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY8046171.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16229..17062)
                     /gene="cysT"
                     /locus_tag="JNY73_000019"
     CDS             complement(16229..17062)
                     /gene="cysT"
                     /locus_tag="JNY73_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY8046172.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17062..18078)
                     /locus_tag="JNY73_000020"
     CDS             complement(17062..18078)
                     /locus_tag="JNY73_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8046173.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18382..19173)
                     /gene="ucpA"
                     /locus_tag="JNY73_000021"
     CDS             complement(18382..19173)
                     /gene="ucpA"
                     /locus_tag="JNY73_000021"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY8046174.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19360..20361)
                     /gene="murR"
                     /locus_tag="JNY73_000022"
     CDS             complement(19360..20361)
                     /gene="murR"
                     /locus_tag="JNY73_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY8046175.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20324..21220
                     /gene="murQ"
                     /locus_tag="JNY73_000023"
     CDS             20324..21220
                     /gene="murQ"
                     /locus_tag="JNY73_000023"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY8046176.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21224..22648
                     /gene="murP"
                     /locus_tag="JNY73_000024"
     CDS             21224..22648
                     /gene="murP"
                     /locus_tag="JNY73_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY8046177.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22828..23727)
                     /gene="yfeX"
                     /locus_tag="JNY73_000025"
     CDS             complement(22828..23727)
                     /gene="yfeX"
                     /locus_tag="JNY73_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY8046178.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(23823..24398)
                     /locus_tag="JNY73_000026"
     CDS             complement(23823..24398)
                     /locus_tag="JNY73_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY8046179.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24459..24908)
                     /locus_tag="JNY73_000027"
     CDS             complement(24459..24908)
                     /locus_tag="JNY73_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY8046180.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(24895..25320)
                     /locus_tag="JNY73_000028"
     CDS             complement(24895..25320)
                     /locus_tag="JNY73_000028"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY8046181.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25534..26403
                     /gene="amiA"
                     /locus_tag="JNY73_000029"
     CDS             25534..26403
                     /gene="amiA"
                     /locus_tag="JNY73_000029"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY8046182.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26407..27306
                     /gene="hemF"
                     /locus_tag="JNY73_000030"
     CDS             26407..27306
                     /gene="hemF"
                     /locus_tag="JNY73_000030"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY8046183.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27312..28365)
                     /gene="eutR"
                     /locus_tag="JNY73_000031"
                     /pseudo
     CDS             complement(27312..28365)
                     /gene="eutR"
                     /locus_tag="JNY73_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28450..28911)
                     /gene="eutK"
                     /locus_tag="JNY73_000032"
     CDS             complement(28450..28911)
                     /gene="eutK"
                     /locus_tag="JNY73_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY8046184.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(28924..29583)
                     /gene="eutL"
                     /locus_tag="JNY73_000033"
     CDS             complement(28924..29583)
                     /gene="eutL"
                     /locus_tag="JNY73_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY8046185.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29593..30480)
                     /locus_tag="JNY73_000034"
     CDS             complement(29593..30480)
                     /locus_tag="JNY73_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY8046186.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(30501..31862)
                     /locus_tag="JNY73_000035"
     CDS             complement(30501..31862)
                     /locus_tag="JNY73_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY8046187.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTSTVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            32051..33256
                     /locus_tag="JNY73_000036"
     CDS             32051..33256
                     /locus_tag="JNY73_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY8046188.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWVNLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            33762..34478
                     /locus_tag="JNY73_000037"
     CDS             33762..34478
                     /locus_tag="JNY73_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8046189.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQNKAAAIAEF
                     KELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAEEDLRFDSKEEQLPEER
                     EQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLYTWMVSKCRYRGNDKYE
                     IWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGKCGKTYRLRIPSKGTAE
                     EASMTAQQHEREAKVSYLVH"
     gene            35529..36281
                     /locus_tag="JNY73_000038"
     CDS             35529..36281
                     /locus_tag="JNY73_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046190.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIYTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            37041..37436
                     /locus_tag="JNY73_000039"
     CDS             37041..37436
                     /locus_tag="JNY73_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8046191.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHKGKFKVTLTYQKACYIYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            37454..37717
                     /locus_tag="JNY73_000040"
     CDS             37454..37717
                     /locus_tag="JNY73_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046192.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            37740..38282
                     /locus_tag="JNY73_000041"
     CDS             37740..38282
                     /locus_tag="JNY73_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046193.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            38747..38953
                     /locus_tag="JNY73_000042"
     CDS             38747..38953
                     /locus_tag="JNY73_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046194.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            39412..39750
                     /locus_tag="JNY73_000043"
     CDS             39412..39750
                     /locus_tag="JNY73_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8046195.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            39731..40009
                     /locus_tag="JNY73_000044"
     CDS             39731..40009
                     /locus_tag="JNY73_000044"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046196.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            40002..41462
                     /locus_tag="JNY73_000045"
     CDS             40002..41462
                     /locus_tag="JNY73_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8046197.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            41484..42305
                     /gene="murI"
                     /locus_tag="JNY73_000046"
     CDS             41484..42305
                     /gene="murI"
                     /locus_tag="JNY73_000046"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY8046198.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(42424..43118)
                     /locus_tag="JNY73_000047"
     CDS             complement(join(42424..42872,42872..43118))
                     /locus_tag="JNY73_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8046199.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(43642..45015)
                     /gene="eutA"
                     /locus_tag="JNY73_000048"
     CDS             complement(43642..45015)
                     /gene="eutA"
                     /locus_tag="JNY73_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY8046200.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(44990..45469)
                     /gene="eutP"
                     /locus_tag="JNY73_000049"
     CDS             complement(44990..45469)
                     /gene="eutP"
                     /locus_tag="JNY73_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY8046201.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIVVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(45482..45817)
                     /gene="eutS"
                     /locus_tag="JNY73_000050"
     CDS             complement(45482..45817)
                     /gene="eutS"
                     /locus_tag="JNY73_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY8046202.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(46110..48389)
                     /gene="maeB"
                     /locus_tag="JNY73_000051"
     CDS             complement(46110..48389)
                     /gene="maeB"
                     /locus_tag="JNY73_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY8046203.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            48678..49628
                     /gene="tal"
                     /locus_tag="JNY73_000052"
     CDS             48678..49628
                     /gene="tal"
                     /locus_tag="JNY73_000052"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY8046204.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            49648..51651
                     /gene="tkt"
                     /locus_tag="JNY73_000053"
     CDS             49648..51651
                     /gene="tkt"
                     /locus_tag="JNY73_000053"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY8046205.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATCLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(51754..52761)
                     /locus_tag="JNY73_000054"
     CDS             complement(51754..52761)
                     /locus_tag="JNY73_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY8046206.1"
                     /translation="MLPTSLVWAAPAQRAFSDWQVTCNNQNFCVARNTGDHNGLVMTL
                     SRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDKWRISPWLLVTDD
                     TATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVGSETAWIKKGDEP
                     PLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSLDPLRREVNVTAL
                     TDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQETNELELMNATF
                     DEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNWHGPDARPTLWIT
                     R"
     gene            complement(52922..53497)
                     /gene="nudK"
                     /locus_tag="JNY73_000055"
     CDS             complement(52922..53497)
                     /gene="nudK"
                     /locus_tag="JNY73_000055"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY8046207.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(53565..55533)
                     /gene="aegA"
                     /locus_tag="JNY73_000056"
                     /pseudo
     CDS             complement(53565..55533)
                     /gene="aegA"
                     /locus_tag="JNY73_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            55739..57456
                     /gene="narQ"
                     /locus_tag="JNY73_000057"
                     /pseudo
     CDS             55739..57456
                     /gene="narQ"
                     /locus_tag="JNY73_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            57658..60771
                     /gene="acrD"
                     /locus_tag="JNY73_000058"
     CDS             57658..60771
                     /gene="acrD"
                     /locus_tag="JNY73_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY8046208.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(60870..60929)
                     /gene="ypfM"
                     /locus_tag="JNY73_000059"
     CDS             complement(60870..60929)
                     /gene="ypfM"
                     /locus_tag="JNY73_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY8046209.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            61310..61666
                     /locus_tag="JNY73_000060"
     CDS             61310..61666
                     /locus_tag="JNY73_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY8046210.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            61670..62797
                     /gene="dapE"
                     /locus_tag="JNY73_000061"
     CDS             61670..62797
                     /gene="dapE"
                     /locus_tag="JNY73_000061"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY8046211.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            62825..63025
                     /locus_tag="JNY73_000062"
     CDS             62825..63025
                     /locus_tag="JNY73_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY8046212.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(63135..63833)
                     /gene="ypfH"
                     /locus_tag="JNY73_000063"
     CDS             complement(63135..63833)
                     /gene="ypfH"
                     /locus_tag="JNY73_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8046213.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(63907..65922)
                     /locus_tag="JNY73_000064"
     CDS             complement(63907..65922)
                     /locus_tag="JNY73_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY8046214.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(65937..66800)
                     /locus_tag="JNY73_000065"
     CDS             complement(65937..66800)
                     /locus_tag="JNY73_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY8046215.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(66968..67681)
                     /gene="purC"
                     /locus_tag="JNY73_000066"
     CDS             complement(66968..67681)
                     /gene="purC"
                     /locus_tag="JNY73_000066"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY8046216.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVAHRLGVQLD"
     gene            complement(67894..68928)
                     /gene="bamC"
                     /locus_tag="JNY73_000067"
     CDS             complement(67894..68928)
                     /gene="bamC"
                     /locus_tag="JNY73_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY8046217.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(68945..69823)
                     /gene="dapA"
                     /locus_tag="JNY73_000068"
     CDS             complement(68945..69823)
                     /gene="dapA"
                     /locus_tag="JNY73_000068"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY8046218.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            69969..70541
                     /locus_tag="JNY73_000069"
     CDS             69969..70541
                     /locus_tag="JNY73_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY8046219.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            70541..71011
                     /gene="bcp"
                     /locus_tag="JNY73_000070"
     CDS             70541..71011
                     /gene="bcp"
                     /locus_tag="JNY73_000070"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY8046220.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            71264..71881
                     /gene="hyfA"
                     /locus_tag="JNY73_000071"
     CDS             71264..71881
                     /gene="hyfA"
                     /locus_tag="JNY73_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY8046221.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            71881..73898
                     /gene="hyfB"
                     /locus_tag="JNY73_000072"
                     /pseudo
     CDS             71881..73898
                     /gene="hyfB"
                     /locus_tag="JNY73_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            73909..74856
                     /gene="hyfC"
                     /locus_tag="JNY73_000073"
     CDS             73909..74856
                     /gene="hyfC"
                     /locus_tag="JNY73_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY8046222.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLLTLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            74873..76312
                     /locus_tag="JNY73_000074"
     CDS             74873..76312
                     /locus_tag="JNY73_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY8046223.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            76324..76974
                     /gene="hyfE"
                     /locus_tag="JNY73_000075"
                     /pseudo
     CDS             76324..76974
                     /gene="hyfE"
                     /locus_tag="JNY73_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
     gene            76979..78559
                     /gene="hyfF"
                     /locus_tag="JNY73_000076"
     CDS             76979..78559
                     /gene="hyfF"
                     /locus_tag="JNY73_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY8046224.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            78549..80263
                     /gene="hyfG"
                     /locus_tag="JNY73_000077"
                     /pseudo
     CDS             78549..80263
                     /gene="hyfG"
                     /locus_tag="JNY73_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            80273..80818
                     /gene="hyfH"
                     /locus_tag="JNY73_000078"
     CDS             80273..80818
                     /gene="hyfH"
                     /locus_tag="JNY73_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY8046225.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            80815..81573
                     /gene="hyfI"
                     /locus_tag="JNY73_000079"
     CDS             80815..81573
                     /gene="hyfI"
                     /locus_tag="JNY73_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY8046226.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            81566..81979
                     /gene="hyfJ"
                     /locus_tag="JNY73_000080"
     CDS             81566..81979
                     /gene="hyfJ"
                     /locus_tag="JNY73_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY8046227.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            82009..84021
                     /locus_tag="JNY73_000081"
                     /pseudo
     CDS             82009..84021
                     /locus_tag="JNY73_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            84043..84891
                     /locus_tag="JNY73_000082"
                     /pseudo
     CDS             84043..84891
                     /locus_tag="JNY73_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(84929..85990)
                     /locus_tag="JNY73_000083"
     CDS             complement(84929..85990)
                     /locus_tag="JNY73_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY8046228.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            86203..87666
                     /gene="bepA"
                     /locus_tag="JNY73_000084"
     CDS             86203..87666
                     /gene="bepA"
                     /locus_tag="JNY73_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY8046229.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVLQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            87687..88046
                     /gene="arsC"
                     /locus_tag="JNY73_000085"
     CDS             87687..88046
                     /gene="arsC"
                     /locus_tag="JNY73_000085"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY8046230.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(88179..88925)
                     /gene="hda"
                     /locus_tag="JNY73_000086"
     CDS             complement(88179..88925)
                     /gene="hda"
                     /locus_tag="JNY73_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY8046231.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(88975..90264)
                     /gene="uraA"
                     /locus_tag="JNY73_000087"
     CDS             complement(88975..90264)
                     /gene="uraA"
                     /locus_tag="JNY73_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY8046232.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(90350..90976)
                     /gene="upp"
                     /locus_tag="JNY73_000088"
     CDS             complement(90350..90976)
                     /gene="upp"
                     /locus_tag="JNY73_000088"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY8046233.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            91301..92338
                     /gene="purM"
                     /locus_tag="JNY73_000089"
     CDS             91301..92338
                     /gene="purM"
                     /locus_tag="JNY73_000089"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY8046234.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            92338..92976
                     /gene="purN"
                     /locus_tag="JNY73_000090"
     CDS             92338..92976
                     /gene="purN"
                     /locus_tag="JNY73_000090"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY8046235.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            93148..95214
                     /gene="ppk1"
                     /locus_tag="JNY73_000091"
     CDS             93148..95214
                     /gene="ppk1"
                     /locus_tag="JNY73_000091"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY8046236.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            95219..96760
                     /gene="ppx"
                     /locus_tag="JNY73_000092"
     CDS             95219..96760
                     /gene="ppx"
                     /locus_tag="JNY73_000092"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY8046237.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(96799..>97907)
                     /gene="pdeF"
                     /locus_tag="JNY73_000093"
     CDS             complement(96799..>97907)
                     /gene="pdeF"
                     /locus_tag="JNY73_000093"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY8046238.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        23763 a        25611 c        24696 g        23837 t
ORIGIN      
        1 ctatctgcga tactcaaaga ttcgaacctg cagcaggttt gagttgagcg cagcgaaaca
       61 acggagccgc tcgcggcgac ggcccgaagg gcgagcgaag cgagtcatcc tgcacgaccc
      121 accaatgcaa aaaagcgccc taaaggcgct tttttgctat ctgcgatttg taagattgat
      181 ttttcttctc aagttccttg ttcttctacc gatattacct cgtacagtga cgaggactga
      241 acaatggcca ctatctcttc aacttcaatt ccttccatac aaacacagag tagtaacagg
      301 gcatcacaag gtagcgatgt tgcttcacaa atcgcgcgga taagtcagca aatcatcaaa
      361 ctcactcaac aaattaaaga gattgtagac actagcggca gtgctgaaga caagcaaaaa
      421 caggccgaat tgattcaaca gcaaatcacc ctactggaaa cacaactggc gcagttgcag
      481 aaacagcagg cagaaaaggc gcaggagaaa gagcagcgtt tgtctctgaa tgtatccctt
      541 ctaaatcctg ttgaaaaaac gactcatatc gatatctata tctgatacaa cggatcccct
      601 tcccgaccag ccattagcgc attgccctgg tctcgtacca cgtcccataa cgcttcagcg
      661 gcggtggaaa gtgaacggtt cttcctgcgc accagcatca actggcgttc aaccaccggc
      721 gtaatgcgct taaccaccag tgggctacct tcaggcaatg gtagcgccag tgcaggaaga
      781 atactgatgc cgatgcctgc ggctaccatc gggaacagcg tcgccggatg tccaatctcc
      841 tgcacaatat tcgcctgaat accattgcgc gccagcgctg cgtcaatcag cggtcggctg
      901 cctgacgcgt aatcctgcaa caccaatttc gctccctgta atgcctgcca gggtacataa
      961 tcttccacgg ccaaagcgct atcgcgatgg caaagcagaa agaaaggctc ggaaagaatc
     1021 gcttcacatt gcagatcgcc cactgggcct ggatcgatga cgatgccaaa gtcgacatct
     1081 ccctggcgaa tactttccat cacccactgc tgcggacgat catgcaggac aaactgaata
     1141 tctggatagc ggcgatggct ttcagcaatg cattgcggaa taagatgcgc ggaaatggtc
     1201 tggctggcag cgacccgaac ttttccgctc agttgttgcc ccatacgccc ggtatcgcgc
     1261 aacgtgctgt taagttcatc cagcagtcgc tcaagacgca aagccaactg ctgccctgca
     1321 tctgtaagca ccacttcacg cgtggttctg tccagcaggc gaacaccagt atgattttcc
     1381 agctccttca cactgtgact tactgccgac tggctcaagc cgatacgctc tcctgcacga
     1441 ctaaaacttt tctcctgcgc tactgtgacg aaaaccttta attgttttaa agaataattc
     1501 atctgttttc ttcatgaatg gatgcaataa atcaatttta tttctcaaac tggaagaagc
     1561 acaatagaac catcgatcat ctggagtctt tatgaaactt tttcgtatcc tcgatccttt
     1621 caccttaacc ctgatcacgg tggtgttgct ggcctctttc tttccggcca gaggcgattt
     1681 cgtccccttc tttgaaaatc tgaccaccgc agctattgcc ctgctgttct ttatgcacgg
     1741 cgcgaagttg tcgcgtgagg cgattattgc tggcggtggt cactggcgac tgcatttgtg
     1801 ggtaatgtgc agcaccttcg tgctgtttcc gattctgggt gtactgtttg cctggtggaa
     1861 accggtaaat gtcgacccga tgctctactc cggttttctc tacttgtgca ttctcccggc
     1921 taccgtgcag tctgcaatcg ccttcacgtc aatggcgggc ggtaacgtcg cggcggcggt
     1981 ttgttctgcg tcagcatcca gcctgctggg gattttcctt tcaccattgc tggttggtct
     2041 ggtgatgaac gttcacggtg caaggggcag ccttgagcag gtcggtaaaa ttatgctgca
     2101 actgctgctg ccgtttgtgt tggggcatct ttcccggccg tggattggtg actgggtgtc
     2161 gcgcaataaa aaatggattg cgaaaactga ccagacgtcc attctgttgg tggtttatac
     2221 agcgttcagc gaagccgtcg ttaatggtat ctggcataaa gttggctggg gatcattgct
     2281 gtttatcgtg gtggtcagct gcgttcttct ggctatcgtg attgtagtta acgtctttat
     2341 ggcacgccga ctgggcttca ataaggcaga tgaaattact atcgtctttt gtggttcgaa
     2401 aaagagtctg gcaaatggca tcccgatggc aaacattctg ttccccacat cggtgatcgg
     2461 tatgatggtg ctgcccctga tgattttcca tcagatccaa ttgatggtct gtgcggtgct
     2521 ggcgcgtcga tacaaacgcc agaccgaaca gttacaggcg cagcaggaaa gcagcgccga
     2581 taaagcttaa agcggacgct tcaggggctg gaccagttgc gtcagcccct cggttttaat
     2641 cagcagcgtg atttgcatta gctcaccgag cttccctgcc gggaactcat ctttgcgggc
     2701 aaaccacagc agatactcct ccggcaggtc aattaagcga cgccctttgt atttgccaaa
     2761 cggcattatc gtattggcta tttcaatcag ctgctctttt tccatctcag ctacccagca
     2821 aacgcagcat ttccgcttcg tcgatgactt caatgcccag ttcctgcgcc ttcgccagtt
     2881 tagatcctgc agcttcaccc gctatcacca gatcggtttt cttcgacacg ctgcccgcga
     2941 ctttcgcgcc cagttcgacc agtcgagctt tagcgtcatc acgcgacatc tggcttaagc
     3001 tgcccgtaag caccacggtt ttaccagcaa acgggctgtc aatctcttcc gcgttgataa
     3061 cgatcggcgc aggccagtga acaccttccg ccaacagctc gctgatgaca ttgcggttgc
     3121 tttcttcggc aaagaagttg tgaacgtggg atgcaacgac aatgccaaca tcaggcacct
     3181 tttgcagctc ttcaatcgaa gcggcttcca gcgcttccag cgtgccgaaa tatgccgcca
     3241 gacctgctgc ggtggcctcg ccgacttcac ggatgccaag tgcatagagg aagcgagcaa
     3301 aggtggtttc tttcgctttt tccagcgcgt taaccacgtt ttgtgccaat tttggcccca
     3361 tacgctccag tccggtcagt ttgcctgcgg tgagtttgaa cagatctgcc ggagtatgga
     3421 catattcttt ttcaaccagc tgatcgatga ttttgtcgcc cattccgtca acatccatcg
     3481 cacggcggga aacaaagtgt ttcagcgact ctttacgctg cgcaccgcaa atcaggccac
     3541 cggtacagcg ggcaaccgct tcaccttcca cacgctcaac gtcagaacca cataccggac
     3601 aatgcgtcgg gaatacaacc tcacgggtat cttccgggcg ttcagaaagc acgacgttaa
     3661 ccacctgcgg gatcacgtcg ccagcgcggc gaatcaccac tttatcgcca atgcgtaaac
     3721 caagacgttc gatttcatcc gcattgtgta aggttgcgtt actcaccagc acgccagcaa
     3781 catggacagg ttccagacgc gcaacaggcg taatcgcgcc agtacgccca acctgaaact
     3841 cgacgtcacg cacaaaggtc atcagctcct gcgccgggaa tttaaacgct accgcccagc
     3901 gcggggcacg cgcgacaaag ccaagctgct cctgctgttc cagtgagttg accttaatca
     3961 ccacgccgtc gatatcaaag cccagcgtcg ggcggtcttc ttccactttg tgatagaacg
     4021 ccagcacttc ttccgccgat tcacaaagcg ttacccgatc gctgaccggc aacccccact
     4081 ttttaaattg cagtaaacgg ccaagatgag tatccggcag ctcgccacct tccagaacac
     4141 caacgccata gcagaaaaaa gtgagcggcc gcttcgctgt aatacgcgga tcaagctgac
     4201 gcagtgaacc agctgccgca ttacgtgggt tagcaaaaac tttcccaccc gtgcgtcgcg
     4261 catcttcgtt aatcttttcg aaccccgcct gcggcaggaa cacttcacca cgcacttcca
     4321 gacgcgccgg gatattctct ccgtgcagct tcagcggaat ggcgcgaata gtacgcacat
     4381 tagacgtgat atcttccccg gtggagccat cgccacgggt cgcggcactg actaaaacgc
     4441 cattttcata cagaatactg acggcaaggc catccagctt cagctcacag caccaggtga
     4501 ctttctcgtt gtttttcaga cggtcctgca cacgtttgtt gaaagcaaga aagctttctt
     4561 catcaaaaac gttatccagt gacagcattg gtacttcatg gcgtatctgg ctgaaagctg
     4621 ccagcggcgc agcacctaca cgttgggtag gcgaatcagg cgtaatcagt tctggatgtt
     4681 tggtttccag ctcgcgcagt tcgcgcatca gcctgtcgta ttcagcgtcg ggaatttccg
     4741 gcgcatccat cacatgataa agatattcat gatggcaaag cgtcgttcgc agttctgtca
     4801 gttgttgttc gattgattcc atatcgcacc atcaatgcta aaaacccccg acaagcgggg
     4861 gttcgaagag gagttaattt gccttaagtg tatcaggcgt tggcgtcttt gacttcgcgg
     4921 atgatgtcct ggtactcgcg caatttctgc ggagtcatca tacggcgctg atcgtcaagc
     4981 acgacaccgc ccacttcatc ggcaatatgc tgcgcagatt gcagcatcag cttgaagttc
     5041 tgcagctcgt caccgtaaga cggtacctgc ataaagatag tgacacccgg agtagtgaaa
     5101 tccttcattt caggatcaaa ggttcccggt ttcaccatat tcgccaggct gaataacgcc
     5161 gggccgctgc catccgggct aagatgacga tggtaaatat tcatatcgcc aaaaatgaag
     5221 cccgcttgtt gaatgctgtt aagaagcagt tcaccgttta gctcgctacc gtgatgcgcc
     5281 gcgacgttca tgataatcac cgcttctttg cgcttcggtt tatccataac tggagccggt
     5341 tccgctacag gctcaggctg tggtgccgct acgggttctg caggctggaa agcctgttgt
     5401 gccggttgcg gtgctgaatg cactggctgc ggcgctggcg cgacctgtgg cgaaactggc
     5461 tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg gtgttgcggc
     5521 gacggacgag cagcctcatg ctcctgagcg ttagccgggg catgattcac gcggtgaaca
     5581 cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt
     5641 tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg
     5701 gtccagaaac cgtgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc
     5761 agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa
     5821 catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta ttaactttca
     5881 cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc
     5941 cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc
     6001 atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta ctattttgcg
     6061 caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt accgctgctg
     6121 gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacacagct cgatgtctgg
     6181 atcccgactt tcatgagtta cgttccggac tggctacaat ggctgagtta tctgttgtgg
     6241 cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctccac gattgctaac
     6301 tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg attgactggc
     6361 gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg aatcatgaaa
     6421 cgcgaatggc aaaaatttgt ctggtatctg ccgcgcgcaa ttgtattact aattctttac
     6481 ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg
     6541 atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc gtttaaagag
     6601 atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc
     6661 ctgtttacga tgatcccgct gcttaatctg ttcatcatgc ccgttgccgt ttgtggcgcg
     6721 acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta acaatctacc
     6781 ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc
     6841 ttgcatgtca ttatttccct tctgtatata gatatgctaa atccttactt ccgcatattc
     6901 tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag
     6961 taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg
     7021 catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa
     7081 gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg
     7141 cgttgaactg gttgaaccga ccagcggtaa taccgggatt gcactggcct atgtagctgc
     7201 cgctcgcggt tacaaactca ccctgaccat gccagaaacc atgagtattg aacgccgcaa
     7261 gctgctgaaa gcgttaggtg caaacctggt gctgacggaa ggtgctaaag gcatgaaagg
     7321 cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca
     7381 acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggagatctg
     7441 ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct
     7501 gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc
     7561 cgttgagcca accgattctc cagttatcgc ccaggtgctg gcaggtgaag agattaaacc
     7621 tggcccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc tcgatctcaa
     7681 gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cccgtcgtct
     7741 aatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg ccgcagcgtt
     7801 gaaactacaa gaagatgaaa gctttaccaa caagaatatt gtggttattc taccatcatc
     7861 gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga aagaattgca
     7921 acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg
     7981 gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa
     8041 ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg
     8101 attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg
     8161 tcagcggtca acacccgcca gcaatggact gtattgcgct cttcgtgcgt cgcgtctgtt
     8221 aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac
     8281 aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct
     8341 ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact
     8401 tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct gtttaaactg
     8461 cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg cgaagacgag
     8521 cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc
     8581 ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg
     8641 gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa
     8701 aaatttctgc cgaccaggtt gctcaggaag ttgaacgttt tctgagcggt cgtgccaagg
     8761 catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt gaagaaaaag
     8821 aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag caggaaatca
     8881 tagccctgat taaagataaa cacatgacag ctgacgcagc tgctcatgaa gttatcgaag
     8941 gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt gcggctgacg
     9001 tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt atcgacctga
     9061 gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc gaaaccgcac
     9121 agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt acttcccaca
     9181 cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt agcgtcacct
     9241 ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag gtttacgtca
     9301 atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa
     9361 aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt caccaggtag
     9421 aagtatgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt aacggcgctg
     9481 aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg ctgcccactg
     9541 aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg caggcggtta
     9601 tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac ttcccgaaag
     9661 aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt aaagagatcc
     9721 tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt cggtaaattg cgcattatgt
     9781 tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc gaaatctaca
     9841 aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg
     9901 tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt gatttcttta
     9961 gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt aatgatatga
    10021 tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag caagttattg
    10081 atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct ggcgatgaac
    10141 gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc gccatttcta
    10201 tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag gtgttagcag
    10261 agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac aagttcattg
    10321 aagaaaaaac aatctgctaa tccacgaaat gcggcccaat ttactgctta ggagaagatc
    10381 atgggtttgt tcgataaact gaaatctctg gtttccgacg acaaaaagga taccggaact
    10441 attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc
    10501 gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg taacaaaatg
    10561 gtcgcgcctg tagacggtac cattggtaaa atctttgaaa ccaatcacgc gttctctatc
    10621 gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa
    10681 ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc
    10741 attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt
    10801 atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt
    10861 gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat ggcgcccatc
    10921 ggcgccattt tttatgcttc cgccagcggc ggcaaaatca attcatcgct ctcatgctgc
    10981 tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac ggtgcacagc atcgtttaac
    11041 gccttccctt tcagcaaacc actgataagc tgagcacaaa acaggtcgcc agtccctttc
    11101 aggtcggttt ttacccgtga atgggaaatg acattcacgc tgtcggcact gaccaccacc
    11161 acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac ccattttaat
    11221 gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg gcaattttta
    11281 ccggtcagga tttccagctc aaagatattg ggggtaattc cctgcgccag cggcagtaaa
    11341 tattgtcgat acgcctcggg aaggtcaggt ttgacataaa ttccgctatc aatatcgcca
    11401 atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc agtcagccac
    11461 tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac agcacgaagt
    11521 tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca ttcgtccgga
    11581 atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc tcagcaatac cgtcggcacg
    11641 gcaaagacat tcaagccgtt ctgttttata gcaggcaccg caatgctgtt gcctacgctt
    11701 ccgtacacta cctgcgactg cacggcgacg atatccgcct gcagcgccct actcttatcg
    11761 ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc tcatcgtatt
    11821 ccaaccgaaa ctttacctga ttctggcagt caaatcggct attacaaaac aaagataagg
    11881 taattcagtg aagaaaatca tttgtctggc cattacgctg ctaatgacac tccccgctta
    11941 cgcgaagtta actgttcatg aagaagcccg catcaacgcc atgctggagg gattagcaca
    12001 gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat acctgcgatg aagcggtttc
    12061 ccacctgcgt ctgaagctcg gcaatacccg taaccgcatt gacactgccg agcagtttat
    12121 tgataaggta gcttcgtcgt catcgattac cgggaagccg tatatcgtga agatccccgg
    12181 taagagcgat gagaacgcac agcctttttt acatgcgtta attgcgcaga cggataaaac
    12241 ggtgcctgcg gaaggaaatt aatccgcttt ggtaaggcat ttacaggagg taacatgaaa
    12301 aaatgcttta tttatcacga tgaaaaatcg aataaatttt ggtggataga ttacgaaggg
    12361 gatagtttag ctgtcaacta tggcaaggta ggtagtattg gtaaattcca tacaaaagaa
    12421 ttcgataatg aagaacagtg tctgaaagaa gccagtaaat tgattgccgc aaaaatgaaa
    12481 aaaggatatc aagaaactcc agactttaac ttcatggatc tctactattt tgatgatgaa
    12541 gagattgggt tacatcttaa aacgtcacac ccaaacttcc agtgccattt tactgatcca
    12601 ctttatatgt gttgctggga tgaagaatct ccttttggta gcgatgaagg tgctgatgct
    12661 ctaaacgaac ttgaaaatag cctccgttct ggactgtgct gatttccctc aaatgttaat
    12721 tgaaactata tggggtatga aatacatcgc tatggacagt attcttgaag aggatgtgcg
    12781 tgcgcaatta ctagccgatg aaatgagctc tatccagagc aatatgatta cctacgcgac
    12841 tgcattcggt cagatcaaag tcatgggtaa aatctcccat aaacttaaaa agatgggact
    12901 caatgcacta gcgcgtcatc agcttaccgc aaaaattctt caatggggtg acggtcagga
    12961 ctcaccaata cttcaaaaaa tgattgatga ccttactgct tttccacacg aaaattaaaa
    13021 actacatttg tcggcagcaa caactgccga caaatcagat tattgacaaa gtgcactttg
    13081 tttatgccgg atgcggcgta aacgcctgat ccggcataaa aatattgcta attcaatata
    13141 ttgcagaaac attgtaggcc tgataagcgg agcgcatcag gcagttttgc gtttgtcatc
    13201 agtctccaat gctattaatc cttaaatccc tgccccctga ctaaaatgct cttccccaaa
    13261 cactccggtg gagaggtagc gatcgccacg atcgcagatg atcgctacca ccaccgcacc
    13321 agggttagct tttgccaccc gcagtgctcc agcaaccgcg ccgcctgagc tgacgccaca
    13381 gaatattcct tcccgcaccg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat
    13441 atccagcacc tcatccacca gcgcagcgtt gaaaatcccc ggcagatatt ccgcaggcca
    13501 gcggcgaatg cccggaatac tgctgccttc ttccggttgc aggccaacaa tggtcaccgg
    13561 tttggattgt tcgcgcataa agcgtgaaac gccggtgata gtgccggtcg tccccatgct
    13621 ggagacaaaa tgagtgatgc gcccgccggt ttgctgccag atttccggcc cggtggtggt
    13681 gtaatgcgca taagggttat cgggattatt gaactgatcg agcagctttc cttcgccacg
    13741 attcgccatc tccagcgcca gatcgcgcgc accttccatg ccctgctctt tggtgacaag
    13801 aatcagttcc gcaccataag cacgcatcgc cgcacggcgt tcctggctca tgttgtcggg
    13861 catcagcaat ttcatgcgat agcctttcag cgcggcaatc attgccagcg caatgccggt
    13921 gttaccactg gtggcttcga ttaagacatc acccggttta atttccccgc gcttttccgc
    13981 ctcgacgatc atcgaaagtg ccgcacgatc tttcaccgaa cctgccgggt tattgccttc
    14041 cagttttaac cacacttcac tgccgttatc cggccccatt cgctgcaact tcaccagagg
    14101 cgtattgcct attgtttgtt ctaatgtact cacgatctct atccatacgt ggtgttgcct
    14161 gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca ttaaaaagcc
    14221 cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag gcgctttgtg
    14281 cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac agccgcgcat
    14341 gttgcagacc aacgaataaa cgctcgccac gctgcggggc atcgtcgcca tgcatcacga
    14401 ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac taattgggtg tagtgacctt
    14461 tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gagactggta cggcggctga
    14521 tatccacttc ccacgggcgc aggaagagat ccaccggccc ctgatacgca ggtgtgtagc
    14581 ccagcggcca gcgatgcgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc
    14641 ggttcacttc gcccataaat tcgagtacaa aacgggtcgc tggttcgcgc catacctgat
    14701 tcggcgcatc agcctgttca atattcccct gactcatcac cactacacga tcggctactt
    14761 cggtcgcctc ttcctggtcg tgggtcacaa acacgctggt gaatttcagc tcttcatgca
    14821 gttgacgcag ccagcgacgc agctctttac gcacctgcgc atccagcgcg ccaaacggtt
    14881 catcaagcag cagaatttgc ggttctacag ccagcgcgcg cgccagcgcc acacgctgtt
    14941 tctggccgcc ggaaagctgc gccggataac gatccgccag atgcgcaagc tggaccattt
    15001 ccagcaattt tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg cgacgcggca
    15061 gcaccgtcag gccaaaagtg atattgtcga ataccgtcat atggcggaac agcgcgtaat
    15121 gctggaacac gaaaccgact ttacgatcgc gcgcgtgcag gcgactcacg tcggtgccgt
    15181 ggaagcgaat atgcccgctg gtttgatgct ccagcccggc gataatccgc agcagcgtgg
    15241 ttttcccgga accggacggc cccagcaacg cgaccatctg accagaagga atatccagtg
    15301 agatatcgtt cagcacctgg gtgcgaccaa aagacttctt gatattggca atctcaatgc
    15361 tcatgatgct cctcctgctg cgcgcgtttt tcctgattct ccaggcgcca ctgcaacata
    15421 ctctttaaaa acagggtgat aatcgccatc agcgtcaaca gcgcggcggc ggtaaaggag
    15481 ccgacggtgt tgtagtcctg ctccaacaat tcaatctgta acggcagcga aagtgtttcg
    15541 ccgcgaatcg agccggaaac caccgacaca gcaccaaact cgccaatcgc gcgggcgttg
    15601 gtcagcacca cgccgtaaag cagcgcccag cgaatattcg gcaatgtgac gcgacggaac
    15661 atctgccagc cggacgcgcc aagcaaaatc gccgcttcgt cttcctggct gccctggctt
    15721 aacatcaccg gcaccagctc gcgcaccaca aacggacacg tcacaaagat ggtgaccagc
    15781 accattccgg gccaggagaa cataatttgc agattatgtt catccagcca gccgccgagc
    15841 gggccgttgg agccgtagaa cagcagatac accagaccgg caaccaccgg cgatacggca
    15901 aacggaatgt ccagtagcgt cagcagtaac tggcgtcccg ggaagttaaa gcgcgtcacc
    15961 agccaggcca gcagaatgcc gaacaccagg tttaccggta cggcaatcag cgcgatcatc
    16021 accgtcagcc agatggcgtg cagcatgtcc ggatcggcca gattctgtaa aaccggcatc
    16081 agccccttgc tgaatgcctg cacgaagatg taaatcatcg gcaccagcag gatgaacgcc
    16141 gaaaccagca tcccgatgcc aatcagaaac catttgcccc agttaatcgg gcgcgcgtca
    16201 taacgcttca attgggtaac ttccgccatt aatgacctac cacacgccga ccaaagcgac
    16261 tttgcagagt gttaattgag aacagcagca gcagagatgc cgcgaggatc accgaagcaa
    16321 tcgcgctcgc tgccgggtaa tcaaactcct gtaagcgcac aaaaatcatc agcgacgtca
    16381 cttccgtctt ccacgcgata tttccggcga taaaaatcac cgcgccaaat tcaccaagac
    16441 tacgggtaaa cgacagcgcc acgcccgcca ccagcgccgg agaaagctcc ggcagcacca
    16501 ctttgcagaa actctgccag cgcgttgcac caagcgtttc cgccgcttct tcatattccg
    16561 ggcctaactc ttccagcacc ggctgcacgg tacgcaccac aaacggaatg ctggtaaagg
    16621 ccatcgccac cgcaatacca agccaggtat aggtgacttt gatatcaaac ttcgccagcc
    16681 attcaccgta aaaaccgttt acggaaaaga gcgaggccag cgttaaaccg gcgactgccg
    16741 ttggcagcgc aaagggtaaa tccatcagcg catcaagcag cgtgcggccc ggaaagcgat
    16801 aacgtgttaa aatccacgcc atcagcagac cgaaaacgcc gttaaaaatc gatgccacaa
    16861 acgccgacag cagcgtcact ttgtaggccg cgaccacctg cgggttggtg atcacctccc
    16921 agtactgcgc ccagctcatc tcagaaagct gcatcaccag cgcagagagc ggcagcagca
    16981 atatcaggca cacaaacagc agactggtgc caaggcttaa agtaaagccc ggcagcacgc
    17041 gtcttgaaga aactgcaaac attacttacg ccccgccgct aacagcttgt ctaactcgcc
    17101 gccgctggtg aagtgggttt tcatcacttc cggccatgag ccaaatttgt cttccacgcg
    17161 gaacagttcg gtctgcggga atttatcttt cagtttgtcc atgacttccg ggttattcac
    17221 acggtagtaa tagtcggtga tgatggtctg cgcctgtggg ctgtagagcc agttcaggta
    17281 ggcttttgcg gctttttccg taccgttggc ctgcacattt ttatcgaccc acgccaccgg
    17341 gaattccgcc agaatattgg ttttcgggat caccacttca aagccctgtg cttcatactg
    17401 tttacggatg ttgttcactt ccgattcaaa gctaatcagc acatcgccca agccacgctc
    17461 ggcaaaagtg gtggttgcgc cacgaccgcc ggtatcgaac acttcaacgt ttttcaggaa
    17521 ctgggtcata aactgttcgg ttttggcttc atcgccaccg tcagctttat ccgctgcgcc
    17581 ccatgccgcc agataggtat aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag
    17641 cttcacgtcg gagcgcacca ggtcgttcca gtcgtggata ttcttcgggt tgcccttacg
    17701 caccaggaag cccatggtgg agtagaacgg cgagctatta ttcggcaggc gcgactgcca
    17761 gtcagccggg atcagcttgc ctttgtcgtg caggatttgt acgtcggtca cctggttata
    17821 agtgacaacg tctgctttta agccctgcaa aatcgccagc gcctgttttg atgacccggc
    17881 atgagattgt tttatcgtca gtttgtcgcc gccgttatct tttgcccatt gctgttcaaa
    17941 cggcggattc agggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc
    18001 cgttgcctgt acatggcccg ccagcagcag agaagcgacc agcgcgagtg agttcttttt
    18061 cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat
    18121 atttataacg gacgtgaaag gagtaacgat tttatatacc gtttggtgat ttggaagtta
    18181 aaaagggaat aagaacttgg ttcaacaggc gtaattgttg caggcagttt ggacacagac
    18241 agcgcggaga aaccggagcg tacacatagt acgtgaggat ttcgagcact gcccggggcc
    18301 aaaatgacaa atgaaatagc ctggtgaact tagttcaaga cgaaatcctc cccacacatg
    18361 cagggaggaa acagaggtga atcagatacc gacgctaacc gtctccggca gtgtgctgcc
    18421 gccatcaatc acattctgtg taccggttaa atagctggat tcatccgatg cgaggaaggc
    18481 cgccagttcg ccgacttcca gcggatcggc gaggcgacac agcgggattg cttttgccat
    18541 ttcagtcagc accgattctg gatcttccgg gttcgactgg cgggcaatgc tttccgccat
    18601 tggcgtgcgg acgtatcccg ggcagatggc gttaacgcga ataccggatt gcgcgtactc
    18661 caccgccagc gatttagtca ggccaacaat cgccgctttc gttaaggcat acgccgtttc
    18721 gccaggatcg gccaccatat caccagtgac tgaagacatc atcacaatgc gaccatcttt
    18781 gcgcgcaatc atctccggca gcaccgcctt cgtgacgttc catacgcctt taatattgat
    18841 atcaatatgg aaatcgcgat cttcatcgct catatcgagg aaactgccca gacgacaaac
    18901 gcctgcgtta ttcactagga tatcaatgcg cccttctttt tccttcgcgc gtttgatagc
    18961 tgcggctacc gacgccgggt cacgcacatc ggcgacaacc gccgtacagc gatgaccacg
    19021 accacacagt tcgtccgcta gcttttcgat ctcaggggag atatccagca agattaggtt
    19081 cgcgccatga cgtgcaaaag ttctggcaat tccttcgcca attccctgca atgcgcccgt
    19141 aatcagtgct gtcttgcccg tgagtttacc cattttaatg ctctccttgt taatacagag
    19201 cgttacactc cattccctct gaaaatacag gataaacaaa atattgctgc ggatcagctc
    19261 actaattcag gaaaatctca atccagtcgc tgctttatgc atcatttcag gcgttgtgtt
    19321 agctcactgc tgcgctgaat cattttcaat gacgccacat cattgatgtt gcacaagacc
    19381 aacaaacagt aaatcggtaa cagaattttg cgccgtgcgg gtagacatcg acgagctacg
    19441 ccattcggtt tcaccggaaa cggtatcgag cgtgaaatgc gccagccgcc gcagcggaga
    19501 gtcagccaga gaagtaatgg caatcaccgt tgccccctgc tttcgtgccg cttctgcgca
    19561 caagacaatc tcttttttac tgccgctgta agagatagcg atttgtacat cgcctttttt
    19621 caacgcctgg gaaacggtgg cttgtacgtg cgtgtcggct tcgcaggcca cgcgatagcc
    19681 aattttcatc agtttgaacg acagatcgcg ccccaccagc gccgatccgc ccaggcccgt
    19741 tatctggata aacggtgctt tactgatgac ctcaatgatt ttttgcagcc gcgcgtagtc
    19801 gaacaacgca caggtttgct ccagcgccag ctctttttcg cgattcagtt tgcgggcaat
    19861 cacttccagc gaatcatcac tggtaatcga actgtgcagg tgcagcgccg tggcattcgt
    19921 tttctcccgg ctggcactgt attcgccaat taacgccata cgcaattcag taaatccttg
    19981 cgcgccgagc ttctgggcaa atttcacaat gctcgactgg ctgataccga gctgtttcgc
    20041 catctgccgt gaagagacgg atttcagctc gctcactctg gcccgcagaa agtcagcaat
    20101 tttttgctcg ttcccggtaa attcactttc cgcattgcgt attttcgtca ggtacaacat
    20161 cttccactct cctgggctgg cagcaaaaat tccgcttaag ttcttatgac gctcttcaca
    20221 ctctgcgagt gtagactcaa tgattccttt aagtctgtca attcgggata tatgattcca
    20281 tacatctgca attatttgat cgtaaatagt aaggtcacca ccgatgcaac tagaaaagat
    20341 gattactgaa ggctcgaaca ccgcctcggc tgaaattgac cgcgtatcga cgctggaaat
    20401 gtgccggatt atcaacgatg aagataaaac cgtaccgctt gccgttgatc gcgtcctgcc
    20461 ggatatcgcc gcggcgatcg atgttatcca cgcccaggtc agcggcggcg gtcgtctgat
    20521 ttatctcggt gcgggaacat ccggtcgtct ggggattctg gatgccagcg aatgtccgcc
    20581 cacctacggc gtgaaaccgg gtctggtggt tggtttgatt gctggaggcg aatatgccat
    20641 tcagcacgcg gtggaaggcg ctgaagatag ccgggaaggt ggtgttaacg atctgaaaaa
    20701 tattaattta acggcacagg atgtggtggt tggcattgct gccagcggtc gcacgccgta
    20761 tgtgattgcc ggactggaat acgcacgcca gctcggctgc cgcacagtgg gaatttcctg
    20821 taatccgggg agcgccgttt caaccaccgc tgagtttgcc attacaccga ttgtaggtgc
    20881 cgaagttgtt accggttctt cgcggatgaa agcaggcaca gcgcagaaac tggtgctcaa
    20941 tatgctttcc accgggctga tgattaaatc cggcaaagta ttcggcaacc tgatggtcga
    21001 tgtggtcgcc accaacgaaa aactgcatgt gcgccaggtc aatatcgtta aaaacgccac
    21061 cggatgtaat gccgaacaag cggaagcggc gttaattgcc tgcgagcgca actgtaaaac
    21121 ggccattgtg atggtgctga aaaatctcga tgccgcagaa gctaaaaaac gcctggatca
    21181 acacggcggc tttattcgtc aggttttaga caaggaataa cccatggcca aagagatcag
    21241 cagtgaactt ctgaacacca ttcttacccg tgtcggcgga ccgggaaata tcgccagttg
    21301 tggtaactgt atgacgcgcc tgcgtctggg tgtacatgac agttcactgg ttgatcccaa
    21361 tatcaaaacg ctggaaggcg tgaagggcgt cattttgacc agcgaccagg tgcaggtcgt
    21421 ttttgggcct ggtaaagcgc atcgtgccgc aaaagcgatg agcgagctgc tgggcgaggc
    21481 accggtacag gatgccgcag aaatcgccgc ccagaacaaa cgtcagttaa aagccaaaca
    21541 aacctccggc gtgcaacaat ttctcgccaa attcgccacc atcttcacgc cgctgatccc
    21601 cggttttatt gccgccggtc tgctgctggg gatagcgacg ttaattgcca cggtgatgca
    21661 cgttccggca gatgctcagg gcacgctacc cgacgcgctg aattttatga aggtgttcag
    21721 caaaggtttg ttcaccttcc tagtgattct ggtgggctat aacgccgccc aggcatttgg
    21781 cggcacgggc gtcaatggcg caattattgc cgcactcttt ttgctcggtt ataaccccgc
    21841 tgcaaccacc ggttactacg ccggttttca cgatttcttt ggtctgccca tcgatccgcg
    21901 cggcaatatt atcggcgtgc tgattgccgc gagggcctgc gcccgcattg aaggcatggt
    21961 gcgccgcttt atgccggacg atctcgacat gctgctgacc tcgttaatca ccctgctgat
    22021 caccgctacg cttgcgtacc tgatcattat gccgctgggt ggctggttat tcgaaggtat
    22081 gtcatggctg tttatgcacc tgaacagtaa tccgttcggt tgtgcggttt tagccgggct
    22141 gttcctgatc gccgtggtat ttggcgtgca tcagggcttt attcctgttt acctcgcgtt
    22201 aatggacagc cagggattca acagcttatt tcccatcctt tcaatggcag gcgcgggcca
    22261 ggtgggcgcg gcgctggcac tctactggcg ggcgcaaccg cacagtgcgc tacgcagtca
    22321 ggtacgcggg gcgattattc ccggcctgct gggcgttggt gaaccgctga tttacggtgt
    22381 caccctgccc cgcatgaagc cgtttgttac cgcctgttta ggcggcgcgg cgggcggttt
    22441 gtttatcgga ttgatagcct ggtggggtct gccgatgggc ttaaacagcg cctttgggcc
    22501 gtctggtctg gtggcgctgc cgctgatgac ttccgcacaa ggcatccttc cggcaatggc
    22561 ggtttatgct ggcgggattc tggtggcatg ggtttgcggg tttattttca ccacgctctt
    22621 tggctgccgt aacgtcaatc tggactgata ttatgaaacg gacaatgctc tatctttctc
    22681 tgctggctgt tagttgtagc gtcagcgccg caaaataccc tgttctgaca gaaggtagtt
    22741 gatcaagtat atgcggcgtt aaagcagtaa taaaaaggcc agtcagtaat gttactgcct
    22801 ggcctttaat caatgaatca gaaacgctta cagcaccatc aacttgtcca gcgacggtgc
    22861 gaaataatag ccgccggtta ccggtttggt gaaacgcaac atcgcatcac gtttgccatc
    22921 ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg cgcagtaggc
    22981 gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct ggcgaacaat
    23041 cttcagccct ttgccatctt ctttcagatc aacgcgggtg aggtgagagg tttccggacg
    23101 ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca tctcctgatc
    23161 gtgaacgctc atccggttga gctgcttcag gttgtgctcc caacgctgca caaacacata
    23221 gctgccgccg gcatccacgc cgtctttgat aaccgccact tcgcggcgcg tctcttcacc
    23281 cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcca cccaacggaa
    23341 gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg ccgcctgagc
    23401 gacagagaag tttacgtcgt gacgcagaga aagaatgtgg atcaacacat cgaactgggt
    23461 cgtcggcgca aggcctttac cgtagcccgg aaaatctttc agctcttctg ccccaacgcc
    23521 gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc accgcaccaa gatgcgcgtc
    23581 cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg ccgcacgcag
    23641 ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc aatgttctgg
    23701 caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataagaatg ccacgcaatc
    23761 cgtggcattg ttgagcgcta ttttacgtgt tttttcgcga cggcggtttg ttcagacgca
    23821 aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc gtccgaaggc
    23881 attaaccctt caggaccacg ccattccccg ctgaactggt agctaatatg ttgactgcct
    23941 tcggctttac attcgacggc acgattatca tcaccatcag ctttttggca attgccaaat
    24001 gctttgctgt aaaggtcgct aaacggtgta ccgattttaa caccggtgtc agcaggaata
    24061 tcgctatcca gcacgtcaat gcgactgatc gtgccttgat cgccgttaat caccatcgcc
    24121 acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc ggttttcatt
    24181 ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc ttgcagtggt
    24241 gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggacga tccaaaccag
    24301 ttccacgggt tagcggcaga ccagttaact gacgacatcg tggaacagcc ggtcagcatc
    24361 aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt ggttattcaa
    24421 taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc ttcattaaaa
    24481 caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacaatatc cgccactacc
    24541 agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg ttgccagcaa
    24601 agtaaaacca cctgcgccaa cagcaacaga aaatagagca catgccaggt gcgaggaaac
    24661 gaagcccgcc gaccgctcaa caaaaacgcc agcaccgccg gaataccggg aatcagcccc
    24721 agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc ctgctcgcgc
    24781 gacgcaccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag cacaagccag
    24841 aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat ggacaggatg gaactcagta
    24901 ctcttcatct tcaatcaaac gcttacccag actaagcacg tcggcgtgtt catatcccag
    24961 gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct gaattttcgg
    25021 gcagccgcga gcaatcagct ttttctccag ccggttaagc aacgcattgg caattccacg
    25081 cccacgaaac tctggatgca cgccaagata ataagcagac ccgcgatgcc cgtcataacc
    25141 gcccatcacc gttccgacca cttcaccgtt tacctcagcg accaaaaaca aactgacgtc
    25201 atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggactca gcaagtcgca
    25261 acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc gtatctccat
    25321 ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg tcgaattagc
    25381 caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca actattgaaa
    25441 tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga tacgatataa
    25501 catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc actaaaaaca
    25561 ctcacttcgc gccgccaggt gctgaaagcc ggtttagctg ccctgacgtt gtcaggaatg
    25621 tcgcaagcca ttgccaaaga cgaaccttta aaaaccagca acggacacag caagccgaaa
    25681 gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc caggccacgg cggaattgat
    25741 accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc gattgctaaa
    25801 aacgtccgtt ccattttgcg taatcatggg attgatgcgc gtttaacgcg ttctggcgat
    25861 acgtttatcc cactttacga tcgcgttgaa atcgcccata aacatggcgc agatctgttt
    25921 atgtcaattc atgccgatgg ctttaccaac ccgaaagctg ccggtgcttc ggtatttgcc
    25981 ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc tgtctgaacg cgaaaaccgc
    26041 gccgatgaag ttgccggtaa aaaggcgact gacaaggatc acctattgca gcaagtactg
    26101 tttgatctgg tgcaaaccga tactattaaa aacagcctga cgcttggctc gcatattctg
    26161 aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc cgcgtttgtg
    26221 gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa cctcgtttat caccaacccg
    26281 gaagaagagc ggctgttagg cacggcggcg tttcgtcaga aaatcgccac agcgattgct
    26341 gaaggcgtga tcagttattt ccactggttc gacaaccaga aagcacattc gaaaaagcga
    26401 taagttatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct tcaggatacg
    26461 atttgtcagc agctgaccgc cgtcgatggc gcagaatttg tcgaagatag ttggcagcgc
    26521 gaagctggcg gcggcgggcg cagtcgggtg ttgcgtaatg gtggtgtttt cgaacaggcg
    26581 ggcgtcaact tttctcacgt ccacggtgag gcgatgcctg cttccgccac cgctcatcgc
    26641 ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt gcatccgcat
    26701 aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt ttattgccga aaaaccgggt
    26761 gcagagcccg tttggtggtt tggcggcggc ttcgatttaa cccctttcta tggttttgaa
    26821 gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gccagccatt tggtgaagac
    26881 gtttatcccc gttacaaaaa gtggtgcgac gattacttct acctcaaaca tcgcaacgaa
    26941 cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt cgaccactgt
    27001 tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc aattgtagag
    27061 cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta ccgtcgcggt
    27121 cgttatgtcg agttcaatct ggtctgggat cgcggcacgc tgtttggcct gcaaactggc
    27181 gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga gtatgattat
    27241 cagccaaaag atggcagccc agaagcggcg ttaagtgagt ttattaaggt cagggattgg
    27301 gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca acgtcaacga cggcttctcg
    27361 gcaaacagct gctggtaatc cgtggcaaat tgccccagat gccagaatcc ccactgcatg
    27421 gcggcgtctt ttaccgttgt gctttgcgac cacgggctta tcagttcgcg gcgtacggcg
    27481 ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc ctaaaatagc gtgaaacgcg
    27541 ttttgtagcg tgcggcgact gacatgcagt tgattacaca aatccagcac cgtcaccggt
    27601 tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaaccgacg gtaactctga
    27661 tgactgatgc tttccgccgt caccattggt tgcgcttctt ccagcatcgc ccccatcgcc
    27721 attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt ttccgggttc
    27781 tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt atgctgcttc
    27841 tttcacttcc agcgccgact ggctacgcag catatgtaat acccgatccg gattgtgcaa
    27901 aaagttagcc tgacaggtga tgacatcttc agacagcact acgcccagga tcgtataatc
    27961 gtccggcgta cttaattcaa actcggttcc accggggcgc gttgctattt ctgcgcttcc
    28021 cagacattgc gaaccgataa atccctgctc accgcgcgtc gccggaatgc caaaccagaa
    28081 cgagttcggc cagaccaggc acgactgacg cagcgccaga ccggtgtatt cacgaaaaac
    28141 ctgaatatca tcgagtagaa tttccgtaaa ttcaccatga aacttgcccg gatgcagctg
    28201 atcgtaaatc tgctgccagg cggtaatcgt caaagcatgt tcatagacat ccgttgtctg
    28261 tcgttgatga acattatcca cttcgacctt cggcgtgagc ttcaggtttt cgggtaaggg
    28321 ttcatgataa agatggtgca aattggctgt acgggtcttt ttcatgatgt taatgccggg
    28381 tgttgtagga cacccggcac ctccgacagg ttaatggggc ttgagacgat aacgactact
    28441 gcgtttacgt tacgtcccgg cagaaaagag ctgttcgagc gcatttctgg ctttttccag
    28501 cggccagcca aagtgggcgg ccacttctcc tgccgtcatt ccctgacata ctgatgttaa
    28561 cagcgccaac aactcgtcag cagattccgc gacgataact ggcgcgtcgg gttcctttac
    28621 gggttgcttc ggctggcggt taaagccagt aaccagccac tgggtgtcat cgtctggccg
    28681 accgatctcc ttacggctga tgacacgccc ggtacgcatc gcggcagcac aacctgcgtc
    28741 cagcgccgca cgacacgccg ccagatcgcc ttccaccacc agcgttaagc gaccagggtc
    28801 aagcacttcg tgactgagca gacgaacgtt agctgcttta agcatggcat ccgctgcatc
    28861 tatcgcggcg accatgccgt ccacttccag caatcccagt gcattgatca tcggcaacct
    28921 ccgttacgca cgctggattg gattacgcgc gatttccagc actgcatcgg taaaggcgtt
    28981 acaggctgct ttacacgcgg cctggctacc ggttaaaaat gctgccgagt agttggtttc
    29041 agacggtggc gggacgtagg tcaccagctg tacgtcggca gatttcaacg cggcatcaat
    29101 gccgtaggtc gcttccagcg gcggtgccac cagatacgcc atcggatcgc caagcgtgat
    29161 cccggcggtt gatgaaagat aagaacctgt acgcgaaacc acatgcgcca ggaaggcggt
    29221 gttttctgcg tcattggccc actggaaagc tgcgccattt tcaatatgtg caaccatcgc
    29281 atccagaccg gcacgcactt ccgccgggtt tggcccacca agcataatca gcacttcacc
    29341 ggcagtcggt gacgggccgt gtgccgctcc cgcatacagc gagcgaccat ataccacttc
    29401 gaccatcgct tgtttggtcg cttcgtcagc cgcaatgtag gttacgtcgt cagaatcggc
    29461 agaaatcagc ccgaggctac gaatatgcgg cggcaatttt agttcacgcg caaattcagc
    29521 gttaacggag gcaatcaccc gcatggcggt taccgacggt cgaatcaaat ctaaagctgg
    29581 catgatgcct ccttatcggg tcatgttgat gccggatgct ttctgctcca gcatacgttt
    29641 cgccaaatcc acaatgacgg ctgcggcttc aaccggcggc gtgccgccct ggtgaatgtt
    29701 agagatacag gtgcgatcgg cctcaacggt ggtcgccata cgcggcgagt aaacggcgta
    29761 gcaggagagg ctttctgact gccctaaccc cggacgttcc cccaccagca ggatcaccac
    29821 tttcgcgccg aggatctcac cgatctgatc ttcaatcttc acgcgaccat aacgaacaaa
    29881 gaatggcgtg ccgactttca gcccggcctg tttcaggccc gccatcagcg gcggcaggat
    29941 ctcttcgtag ttgacggtga tggcatcagt tgacaggcca tcagaaataa cgacctgtac
    30001 gtccggatta gcaacacact gtgctttcag cgcttcaaca gcttctgcac acaggcggcg
    30061 gcccatatcc gggcgagtca ggtagagatt tttgtcgcta atttcagagc gtacttccag
    30121 caagccctgc gctttcaccc actcttccgg cacttctttc agtacggtat ctttcgaacg
    30181 agagtgatcg gccaagaaac gcagcagcgc ctgagtacgt ggacgcggac cggcacgacc
    30241 ggtacaaacg cgggccacgg tgctgcggcg cagttctgtt aatacgtctg cgcgatgcgg
    30301 gttttcaaca ccaatccacg cttttgcttc agcggaacct aaatccagcg cacagctttc
    30361 cgaggtcacc ggtgccgcac agttggtggt ggcgcacttt gcttctgacg gcgcgggggc
    30421 cgcttgtccc attgacgcca tcacgctgcg tacaatttct tcaatctgtt tttgatccat
    30481 gatgtgttat ctccgcgtca tcagaagaac agtgacggat cgcccgcccg tttggtcagg
    30541 cgaccgtttg ccataatgcc catgctttcc agccagcgtt caaactctgg tgacgggcgc
    30601 aggttgagta actgacgcac agtggaggta tcgtggaatg cggtggtctg gtagttgagc
    30661 atgatgtcat cacccagcgg catccccatg atgtagttgc agcctgcggt ggcgagcagg
    30721 atcatcaggt tttcgttgag gttctggtca gagtcagcgt ggttggtgta gcagccgtca
    30781 cagcccatag agatgccgct cagcttgccc ataaagtgat cttctaagcc agcacggata
    30841 atctggcggt cgttgtagag atactccggc ccaataaagc cgaccacggt gttgacgata
    30901 aacggatcgt aatgacgcgc cagcccatag ttacgtgctt ccatcgtcac ctggtcagcg
    30961 ccgaagttag cgccagcgga tagcgcagag ccttgtccgg tttcgaagta gaggcagttt
    31021 tccccggcga tacgattgaa ctctgcgccc actgcgcgcg cttcgtcgag catcgccagt
    31081 tccacgccaa actctttcag ccctttttcg ctgccacaga tactctggaa aatcagtccg
    31141 ccaggagcgc cgcgacgaat cgcttcgatc tgggtggtga cgtgcgccag tacgcagccc
    31201 tgagttggga tgttgaattt gtcgatcacg ccataaatgg tatccagcac gcggcttaag
    31261 ttttccacgt cgtcagttac cgggttaacg ccgatcaccg catcgcccac cccgaaggaa
    31321 agcccttcat agatttgcgc agcgatactt tgcacgtcgt cacgggtatc gttcggctgc
    31381 aaacgggcgc taaaggtgcc cggaataccg atggtggtat tggccttttt gattaccggc
    31441 attttcttcg cgccgtagat caggtccgcg ttggagcaaa tcttcgctat cgccgcgacc
    31501 acttccgagg tcagcccttt gcgggtaaag gcaatgtcgt ccacgctggt ttcatcgctc
    31561 agcacatact cacgcagttc gctgatgctc cagtttttaa tctggttgta ggccgtttcg
    31621 ttaacgtcgt cctgaatcag ccgcgtcacg cagtcatctt cataggcaat caccggatta
    31681 ttgcggatgt ccgctacggt catttccgac aacacctgct ttgccgccac gcgctcctgt
    31741 gagcttgccg ctgcaacgcc cgccagcaca tcccccgaac gcagttcgtt ggctttagcc
    31801 agcacctctt ttacatcctt aaactgatat acattgccga acaatgtggt ctttagtttc
    31861 ataagtcgtt ccctcaggaa gaagtgatga gtaacatttc tcacagcctg caaacaattc
    31921 tatttcatcg ttagcccttg tcatgtaagg tgatttcctt aacacgcccg ttacttatcc
    31981 tgccgaacgc aaccgaactc gccggataca tgagcggggt tatcgagtcg ttagatcgag
    32041 gtatccggtt ttgagcaaaa caccactcac agcaaaggcc atagacgccg cacaaccaaa
    32101 ggacaagcaa tacaaactca ccgcttcact gacgcccggc ctgtttttgc tggtgcatcc
    32161 caacggcagc aaatactggc gattccggta ctggatagac aagaaagagc ggttacaggc
    32221 tattggggta tatccgctga ttagcctcaa ggaagcccgc aaacgcgcca cagagagccg
    32281 tttactgata gcccagggaa ttgacccaat ggatttggca cgcaaggaga aagccattga
    32341 tgcactcaac atggcggcag gttttaagac ggtagcggag gactggctgg ctaccagggt
    32401 aggcggctgg agtgagagct atacaaagca ggtcaaatca gcactggaga aagacgttta
    32461 tccggtactt ggcaagcgtt caatcgttga tataaccgcc cgtgacgtgc tggcattact
    32521 ccagaagaaa gaacgtaccg caccggagca ggcccgcaag ctacgccagc gcatcggtga
    32581 gatcttcaag tttgccgtta tcaccgaact ggtaacgagg aatccggttg cagatcttga
    32641 tacggcactg aaggctcgcc gacctggtca caacgcgtgg ataccgatta gtgaaattcc
    32701 ggccttctac aaagcccttg agagggccgg gagcgtccag attcaggcgg caatacgttt
    32761 gcttatatta acggcattaa ggaccgcaga actgcgtttg tgccgttggg aatgggtaaa
    32821 cctggaggac gcaacaatca cattgcccgc tgaagtcatg aaggcccgcc gcccgcatgt
    32881 ggtcccgcta tcacggcagg cggtcgagct attgcaggac cagttcacac gcagcggata
    32941 cagtgttttc gtgttcccgg gccgattcat ggataagcca ttatcagcca gcgcgatact
    33001 caaggcgctt gagcgtatcg gatacaagtc aatagccaca ggccacggct ggcgcacaac
    33061 gttcagcacc gcacttaacg aaagcggcag gtataaccct gattggattg agatccaact
    33121 agcccacgtc ccgaagggaa ttaggggagt ttacaaccaa gcggcctatc tcaaaatgcg
    33181 ccgtcaaatg cttcaggact acgccgacgc gattgattcg atactggctg atggtatcaa
    33241 tccgcttgaa ctgtaatgaa tgattaatca cgttttccga cgactccacc ggagtaggcc
    33301 ggataaacac tggacacctt caaaataact attcactttt gccggattcg tccggctttt
    33361 tttgtctttg ccgatgggat caattattcc aattattcca attattccaa ttattccaat
    33421 tattccagta ttgttggaat aaataaatat caatgactta aagccgtgtt tgcaacaggg
    33481 tggatcgggg atcgaaaata ggatcaacac aaaaacaccc tggaggctac agcacactag
    33541 ggggacacaa aaacaacaca ggaaaaaata ccatgtacca atcagcaacc gcctacgtgg
    33601 cgaaaccagg cgaaacactg gtcaaacaca aaattgcaac actccaagca cttcaaacac
    33661 tccaagccct tccggaacag gttcaggccg cctttcgtga tggcatgaac atcatccgct
    33721 tcgatgggta tcgctgggaa tacaagccag caactggatt cgtgcgcata gatcaaatct
    33781 tgtcgccagc caatgaagag ccagcgctag ccaatgcaga accagcgcag gagcaggagc
    33841 aggagcagga acaggaacag gaacagaata aagcggcggc tattgccgag ttcaaggagc
    33901 tgcaaagaca acaaatcaca gcgcagcaag aagaatgccg ccacccgctg gcaatgccca
    33961 agccagtagc gaaagccaac acagacaagc cgacttcatg gtttaacgaa gaagcggaag
    34021 aagatttgcg ttttgatagt aaagaggagc agttgcctga agagcgggaa caggtggaaa
    34081 cggaagacca gcaaaacgat tatcgcaacc ttccagccgt caataacgct atggcttacc
    34141 tcttcaaaag cgggctgttt cacaagctga aaggcgatga gttaaagcta tatacgtgga
    34201 tggtatccaa gtgcaggtat cgcggtaacg acaaatatga gatttggtat tcccgcacca
    34261 aaatggcaaa agcgttaggt aagagcgttg acacgataga tcgccacctg gaagcgttga
    34321 gaaagctggg acttatccag gaaatgcgcc aggcactgag gggcaaatgt ggaaaaacct
    34381 acagactgcg aatcccgtca aaaggcacag cagaagaggc ctcaatgacg gcacagcagc
    34441 acgaaagaga agcaaaagtc agctacctgg tacactaggc agcgggtcag caaagggggg
    34501 caaatgcccc ttttttcatg tcagataatc agatcattac ctcaataaac taataatcac
    34561 atcgcggtta caaccagttt gatagtgata gctcaaaccc agacgtggcg ctggctgcaa
    34621 gcaaaataag ccgcaaaaat gcggtcctat ttgccagaac cttttattat tcaattggtt
    34681 agtgcaagaa aaagcgtgat tactgttcaa gaagtatcca gaaaggtcat gattttgcca
    34741 tgattttgac gctgaaaaaa aataggtggg catttttgcc cactcactag ccgcaaattt
    34801 gcccactcac tagccgcatt tttgcggccc aaagtaataa atacagagaa ataataaata
    34861 cagatcagcg ccaagatttt tcgtcttttc ggcggaatca agagactagt gcttacgtcc
    34921 tgataacctc cagagcagac cttaacttct gtgcagcagg actacgctca ttgccgctcg
    34981 gctaggtcaa atcctcacga taaagctgtt cggattgtga ccataatgtt aaaggactct
    35041 ttaaatcatt ccttgacgaa ttaactagct ggcttaagcg gaatagcatg caggataaaa
    35101 acaaaaccag cttagcagtg gggagagctt cgcttcgctc gtctcctggg gccaatgctc
    35161 aatgaaacga cgtaaggcac ggagcgctca ccataggtag acacataaac atctgagagc
    35221 gcaccttcta tcgcgctacg cgctcaataa aaaccaacga caccgccaca ctgcaagacc
    35281 aatggttttt atcgcgtata cagaccagac aggaaacagc agcaaagcag cgtatagcca
    35341 ggtgcagaca gcacactcac acgacgcaca gcaggcttaa gtgatcactc aggaaacttt
    35401 atccccgccg ccgatcaccg cgaactatca tgaccgcgcc aaattcgaaa ggaatggccc
    35461 atagaacgcg atgaacacac ataagcgcta acttgcttac ctgaaaactt aacgtcgcca
    35521 cagagcgatt gagagcgctt aaaatcgatg cgatttcatt aaatttttct cactcgcctg
    35581 aatggtggcg atcaggactg atcgcaacat tgccccgcct cgatattgag cgcataatat
    35641 acacatacat taagcggaaa agagataagt ttattgtgaa aatcaggggg ttacacatca
    35701 tgcgcatatc agaacaacaa cggcagatcc tcgactatct ggcaaaaaac gaatcaatca
    35761 ccgcacgaca agccgcagat ctcgtttatg gtggcaatgt tacccgcgtc caggttgacg
    35821 ccgcccgccg ctcactcatg acgatggtta gtaatgggct aatcaggaaa caaggccgcg
    35881 ctttcgtagc tgaaagacat atcaatcacg cttatagcca ggacgttgaa gaattgcgcc
    35941 gccagatcct ttgggtattc agcaaacagg gtcaggagta tttcctttct gaacacccaa
    36001 atttcgccgc caaagagtta acagtaagcg gattgtcgtg cgcactggat tatcttgtcg
    36061 gtgaaggttg cgttacttac aagcagctat ggcacgccct gaatacgctt gtagaagatg
    36121 gtttactcat tgccacgcgt gagaacatcg aatactggat aatgccgcgt tcaggtcgcc
    36181 gcaatcatgc acggtatatt tcaggcaaca gatacagaac gccggacttt aatccgacac
    36241 aggaagatcg ggaccgtgcc gaaatgatgt gcagggaata ggagaaaaga attatggata
    36301 agttattttc gatgcagtga acacaagcgg gcgcaatgcc cgtttttatt gccttattcc
    36361 acgcctgacg aatcaatttg atcacacctg agcaaatgca tagccagaag ttaaggccgt
    36421 tatattgcga ttgggagcgt tttaaacggt atcaatttaa ttaattctga tgtggtgatt
    36481 tgcaggcaaa aaaaacccgc cggagaagcg ggaaatagag acatatcgtt gtagttatta
    36541 acagatcact ttgaggtcga gggcggctaa ccctcgctaa tattatcccg ccattacctt
    36601 gcgggtgcaa ataatctttt attgctttga tgatttaata aatagcactt tttgctaaca
    36661 aaaaccgctg tcaatatatg gccaaaaatg tcaacagaaa aaataaggtt tataatatta
    36721 agtatcacat gggtaatggg ttcagggaat cggaatccac cgcctcacgg aaattcaaaa
    36781 tttgcttctt ctttcctccg tggggcggct tctttttcgt atcgaggtat ttatgaaagc
    36841 aataacatta gaacagttcg cagcaatggc gaatgtatcg accagaacgc tgaggcgctg
    36901 gctaaaaaaa ccatcaaccg gagtcccttt gccatttacc ccacgcggta ctcgccaatt
    36961 attctgggag cacgaatgca aagaatggtt gtacagaaca gaacgataat aatcacgcat
    37021 cacattttga ggtaaacatc atgaccaata tcgaaagatt agaacgctgt gccgatatta
    37081 tgcggcgcag atggatttat gaccctgaag cgggtttatt gtttagccgt gaaacaaaag
    37141 acgttatcaa aggcagcatg accaataaag ggcatttggt tgtaacggta cataaaggta
    37201 aattcaaagt gacactcacc tatcagaaag cctgctatat atacacatac ggcgcttatg
    37261 acgagacatt atatgaagtg caccacgtta atttggacaa acaggataac cgcatcaaga
    37321 atattcgcct gatgccgaaa gaaaagcacc gccggataca taaaaacgtc cgcaaactta
    37381 ttcggctggg ccacacacaa tggcttcaac cgcaattaat tgaacaatca ctataaacac
    37441 agtagtaaat accatgcatt cattagacag ttattttcag cgcacgacag cccctaaatc
    37501 agccgcccag gaacgccgtg aggaattcca tgagaaagtg atgcgttctg ctgattatat
    37561 tgcggacaag ttcgtggaaa cggttcgtcc acttgttgat gaagtagctg ataaacttca
    37621 gtctgaaatg ccggaagata tggaaggcac tgcaaagcgg cgactcattt gcgaattaag
    37681 tcgtcgtttt ggcgtgtcaa taagcgcttt taaataatcg aaaaaagggg ggaaagataa
    37741 tggacgcatt tgaggtactg gcaaataaag gcgctgaact ggtagcacag cgtgataaaa
    37801 ccgccaacga aggggaacgc gcactgctaa ataaacagat taaagcgatc cgtatggccc
    37861 agtttaaatt aatcagtaat gaagttatcg aaacaattaa accgcagatc ccacaaatcg
    37921 ttgctgacgc tattaaagcc gccggactgg agaaacgcat tgccgggcta aaaacgaacg
    37981 cagagaaaat ggagatgcac aaacagagcg gtgctccgaa tcccgatgag tacttctcac
    38041 cggatgttga gtttatggcg caggttgagg aacgtctggg tagctgtctc accgaggaac
    38101 agcgtcgcta ttttgatgga gtggatagtt cggcaggtat tgacctgaac tcgtatttcg
    38161 gtcgtgaaat tgagcatttt gatgccgccc cgtctatgcc ggagccagaa ccagaaatgg
    38221 ccaccgatcc tgaagctgag caacggaaac gcgtattcaa tgcgataggg attcggtact
    38281 gatcggataa aagaaagccg ggaattaccc cggctgactt aacaatgtga ccaatgaaca
    38341 aatcggacaa gacaaacagg ataagcagtc tcgaaacaat gttcgggaac tatattagca
    38401 caagtccgga gaaaatcatc atgttggcac tacgcatcat gcagggcatc gctaagaccc
    38461 tcgcagaaca tgtcttagac ctgaaacatt cgccactgag caaacaggcc atgaagcgct
    38521 aaacgcttcg tctgtgggct gaatattcgc ttggaactat caataaaatc atcgacatga
    38581 aatcggggcc gagcaaccag agcgcggagg aaatggagtt catcaggcgt ttaattctca
    38641 ttcgcagaga cattcatagc cagttacaca gcgtgggaat cgatattaat gatgggacgg
    38701 gcgattaatt ttcggacaag tactatgcgc gcgcgaggag ctgaacatga agcaagatat
    38761 tgcagacagg ctggagatac tcgaaggcca gcgggcagaa gcgaaacaac tacgtaagca
    38821 ggccagaaga gcgcacagga acaacgaagc agagttacta accaaataca tcagtttcac
    38881 caattactgc atctacgagt gttacaagga agatgcagag gactggctgg attcattgcc
    38941 agaacagtac tgagcagaat aacaccacgt catgttgttc tgaggtgagc atatcacctc
    39001 tgttcaggtg gccaaattta gtgtgccact tcagcatcac cgttgaaatc acaataacga
    39061 tttttttgta aatatgctct cacatatgta ggaatagacc aggttggcat tgaaacacat
    39121 ctaccatgac cataataaag taattttttg atatgtttat ggttttatat taattaaaat
    39181 atatctaaaa ttgatagttt taaaattgca aaatccagat tgtgtttgta atgtttaatc
    39241 tgattgtatt gagtggcgtt tattatgaaa aataaatcag gtaattcttg ttatagagaa
    39301 gcagtcgata ttcatagctc acttgagctt tcatttatat tgcataaata tcatttgcgt
    39361 gtagatgaga agaaatctcg aaatgtattc agcgccatac gtgatgagct catccttgat
    39421 ggggattcac gacaaaactt agctactttc tgtcaaacat ggcttgatga tgaaatccgt
    39481 gagttgctta atttagctat tgataaaaac atgatttata aagatgaata tccacaaatg
    39541 gcggaacctg aaggtcgctg tgtgcgtatg ctttccgatt cgtggagttt ccccgattcg
    39601 cgacacacct taggttgctc aaccattggt tcatctgaag ctgctatgct cggccgcttg
    39661 gcgttaaaat ggcagtggtg taaaaaacgc gaagtacaag gtaaatcaac cgaacctgat
    39721 ttgcgatcct gtgcaaatat gctggcataa attctgccgt tactgtgatg tgaaattgtg
    39781 tgaaattcca cttgatgatc atcacctgat aatgaatgct aaagaggttt tgaagcatgt
    39841 cgatgaaaac actattggtg tcgttacgac tttaggtatt acttattttt gtaaagcaga
    39901 ttgcgttagt gtttttaaga taagtgttaa agttaatatt agtttttata tttacatcgt
    39961 aattatatgt ttctatttta aaaatattcg agagtataaa aatgtctaat gctaatatcg
    40021 cacccaaaag taagaaaatg acagtgggga cactcgctat catgaatata actgctgtag
    40081 taagtctacg atttatacct tctgaagcag aatacggctt aggtgccatt ttttactata
    40141 cctttgctgc aattatgttt ttagtacctg ttgcctttgt tgcggctgag ctagctacaa
    40201 catatccaca aaaagggggg gcatttcgat ggataggaga agcatttggg gctcgctggg
    40261 gttttgtagg aatgcttatg acctggctac aagtcatccc ctactttcca acagtactga
    40321 catttggtgc agtctcggtt gcatttattg atgttgatat gggggtggca gaatcaattg
    40381 ccagtaataa atggtatatt ttatttttcg tactatttgt ttactgggga gctatttgtg
    40441 ttgcattacg tggcgttggt attttctcgc aggtatctaa gtggtgcggt attataggga
    40501 caattattcc agctattatc cttgttattc ttggtttctc ctatctgtgg ttcagcggta
    40561 aaccagcggt gatcccatta ggttggggtg atcttttccc taatatagct aacttccaga
    40621 atattgttct tgccgccagc atatttcttg cttatgcagg gatggaaatg aatgcggtcc
    40681 atgttaacga tttagataat ccaactaaaa aatacccaat tgcaattacc atcgcttcta
    40741 taggtactat tgcaatattc ctgctcagta ctttaggggt cgcctttatt attcctacag
    40801 ataaaattag tctaactcaa agtttgttgg ttgcctacga tatgctgttc gaatgggctg
    40861 gagtgccatg gcttggctct gtgatggcat ttatgctagc aattggcgta ctgggtgggg
    40921 tggttacttg gattgcaggg cctaatacag gtgtattagc gatcgcgaaa gctggttatt
    40981 tacctaagtt tttccaaaaa accaatcgtc atggaatggg gcaccatcta atgtttgtac
    41041 aaggtataat tgtctcagtt ctttctgtta cttttgtgat aatgccttca gttcaagcag
    41101 cttttcagat tttatcacag ttaacagtta tgctctattt agttatgtat atgctcatgt
    41161 tcgctagtgc tatctatcta cgacattctc aacctgatac aaaacgccca tatcgagccc
    41221 ctgctttatt tttctgggca acacttggat tcttagggtc ttttctggct tttgtattaa
    41281 gttttattcc gcccgagcaa atcccaactg gtagtccaac cagctatgtt ctttatttgt
    41341 tagtattagt tgccctattt ttattaattc caatattaat ttacgccttc cgaaaatctg
    41401 actggaaaga tccaaactct gatttcgaac cattttcttg ggaaaaaaga actagtaatt
    41461 aaattttcca taagctacaa aagatgaggt taaatatgtt tagtgctgaa tctccaattg
    41521 gtgtaataga ctcaggtgta ggtggattga catcggtaaa ggaaataatt aatttgcttc
    41581 ctggtgaaga tattatctat tgtggagata atatgaatgc accttatggc aaccgttctg
    41641 ccgatgatat catcagtttg actaaaaaaa tgctgacttt tttacagtca aggaatgtca
    41701 aactagttgc tgtcgcatgt aatactatat cctcaacttt ggaatccgaa aaatacagcg
    41761 gatatgctaa gagctttccg tttcctatct tatccatcat tgaacctgca gtagaagatg
    41821 tgattcgcca gcaatataaa aatgttggta ttatcgctac cgagtttact attaaaacgg
    41881 ggtgtcacaa agagctaata aaaaaattga attcaacaat aaatgtattt ggagaaccaa
    41941 gcaaaaatct tgcaatgtta attgaggaag gtaatcttaa tgctcctgca atcttgaatg
    42001 acatcaaaaa acatgttaac catttaattt ccctccatcc agtaaatgaa attattctcg
    42061 gctgtactca ctatccaatt gttcagaatt tttttgaagc agttgcaccg gatataaaat
    42121 ttattaatcc tgcacatgat caggctatct ctattaaaaa tcacttggga cagctaaatt
    42181 tattaaactc ctcaaatata ggaactttgc aaataaatac tagtggaagt atggagatct
    42241 acaaaacggt actaggggaa ttgagcatta cgaaacctca tacattcagt atcaggcagt
    42301 tttaaattta tccggtgcac cgtaaaatcc cgtccttctg aactgcaccc caaaagttgg
    42361 acaccaactg agtaaggtgc attttatgga caaacccagt tacatactgg gtagtgcatc
    42421 caattagtag aacatgtgtt tttcgataaa cgctccgatc actttttcgt ggatctcaac
    42481 tgagcgtgag aagcagattg ttttacgacc caaccgctta atgcgggtgc gtagcgtcag
    42541 attattacgc tcaatgcgtt gggtgaatat tttgccggtc agatgcttat tcttcggcac
    42601 ctcccggcca tagctgcccc agtcatcgct ggtcagcatg ccgatgttga agggtgtaag
    42661 cagtgccagt agctcccggc acgtttgatc ggttcgggga ccaaaagtgt aggccagtac
    42721 accgcctgtt ttggtgttgt acgcgtaccc gagccaatgt tgccgggctt tactgccaac
    42781 gtagctccat tgctcatcaa gctcgcagat aaacgccaca tcagcatggg caacaggcga
    42841 agaagttatt cgctttggcg cgagtttttt aaagtccgga tgacggtgtt actaccaatt
    42901 ttcagtgtcc tggcggtatc gcgaaccccg gcaccattaa aggccatttc agttatcagc
    42961 tctttcatac ccggcttacg tgcttgataa gtataagtga actgaaacac acggtggcag
    43021 tcacggcagc gaagtctgtc acggccttta gggttctgac catggcggta aacctgagct
    43081 gactgacaac ggggacaatg aatattaacg ctggtcatga gataacctca aaagcccgta
    43141 ttatacatca gattcaacta attagaggca tcacccatcc agcattataa aacatactaa
    43201 caataactaa attataataa tcacaactta caatcaaata ataaatgcat aagattaaca
    43261 atgatttttt cgggttatat ttatctaaaa aagcagaata ttatgtaact tattattttc
    43321 taagcgaaaa tttattgtgc ggtcagacta acaaacttgc gtaccacccg aagaacatgc
    43381 gaagatttaa gtattctttc ttttttggtt tttatttttc attttctctt ttcttctctt
    43441 tccggttctc ttctgttctg gttttggttt tttccttttt atttttattc tttcttcagg
    43501 gttatgctaa aacaccaaaa catttttcac cacaaaagca tccaatcatc ttgccagacc
    43561 aatcacctta accggataca ctgccggata taatcccact aacacccagc caccaagcac
    43621 catacctgat aactaccgta ttcaggaagg aaatgcgagt gatttcaccg tcaccggcac
    43681 aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc ggacaatcac
    43741 ttcgtcaatg actgccaacg ggagttgttg tagctgtggg cgcaacaaca tgcccagagc
    43801 tttaccaaag tcctgcccgg ccaccaccag caggggatgc ggattcggaa aacgcgcgac
    43861 gaaatcgacc agcgcgttga tgaccgtcag taccgcggcg taacgcacag gcagcgaggc
    43921 gggaagcgcc agcacgtaca cgtcagtttt gggatcaaga tccagctgaa gcagcgcctg
    43981 ttgccaggca ctcaccagat ccgtttcatc aatcgggatc gccaccggca aattgcgcag
    44041 cggcagttgt acgccctcca gccagattgt gctgccagag agcgaaaggg tatgtgcacc
    44101 cgcgccaatc accgtggcgc atacggtttg cgccggaaac tgcacattca tctcacgcag
    44161 gcgcggatgg tcatgcagcg ccgttgccag aagtgggcca atatcggcaa aacagaacgg
    44221 gtcggcgggc tggtggcgat aacattcgcc cacgcctcca gaaagcgtaa tgatttcggg
    44281 cgtaacacct gcgggcagca aaccggtttg catcaatgcc tgcgcgagcg gcgaaagcgt
    44341 tccgtcaatc acttcgacaa tcagcgccgc catccgccgg gtaacctgca ccagctgcgc
    44401 gccggtcagc gaacgggcgt cagtgcctgc accgaagcac tcatccacaa tcatctgccc
    44461 cggtttatga gcgtaaacca cgcgcccctg gctgtcggtt tccagcaggc gaccaccgac
    44521 gttgagacag gcagtgccgc tgatttttcc ggcatcgaac agggcgtagt tcgcggtgcc
    44581 accgccgata tcgatattca gtacccgaca cagccgttgt tcagaaaggg tttgcgcccc
    44641 ggctccgtga ccggcgatca cggattcgag gtgcggcccg gcgctggcga cgacaaaatc
    44701 gcccagcgac tgtttttcct tcatcacttt ttccatcagc tgtgcgacca ggctttcggt
    44761 aaactggcct tccggcagct gggcaatgat ggtttcgcgg atgcgctggc tttcggtttt
    44821 gtcggctggc acgctggcgg gcacggacgc cgttaccggg atcgattcat cacattcggt
    44881 gatggtaaaa cccagtagat ccgacacttc acgggcttcc ggggtaataa tgctggcgcg
    44941 cagaaccacg gacattgcct gttcgccgcg cgcgtgtgct tcacgaatat cattcgctgt
    45001 gatgagtttt ttcacctgct tcctcctgtt tggtgagaga tgccagataa tccaccagtt
    45061 gctgcacgct ttgcgggtcg tggctgttta gctcgaaaat cggctcttcg aaacccgtct
    45121 caagcaataa ctttcgcgtt gcggcgacgt cagcatctgg catgtccgtt ttactgatga
    45181 cgacgatttg ccgtttgctg acgccaatat ccagcaaccc ggcaggcaac cgactttccg
    45241 ggtcatttgc gccatggaca tagatcagca tgtctacatc ctgtagcgtg gtaattaagg
    45301 cgtgatacca gcggggatgg ctaaaatatt cacccggcgt atcaatatcg cccttatcat
    45361 taaattccac ggcctgtgtt tttctggcga gggtataatt cccctgcaac gcattaaata
    45421 acgttgtttt tcctgccccg acgctaccca caaacgcaat tcgtttcatg aatgcctcgt
    45481 attaactttt ggtcatttca caaagcgtat aatttaataa gcgacctaaa ccgctgacgg
    45541 tttgtgataa cgcttcttct actgcgccca ccgagccata aatcaccaat gcaccgctga
    45601 agcggtcgag aaaaccgata tgcacatcag cggctttcag cgctaaatca ccggcgatca
    45661 tcgccgtttc gccgggagtt agcgtcataa tgccaattgc gcccgcatcc ggaacgccga
    45721 ttttcttcgc cagttcctca ccgggatgcg cgatgagatg cgccagtgtg acctgtttgc
    45781 cgggcacaaa ttcctgaatg atgcgttctt tatccatgag tcgccgcctg tgaaaaactt
    45841 tcgtgtatcg tgacaatttt tcgtcagagt aaataacaaa attcggcaag cgcgtttaaa
    45901 aggtgaggta gatcactaag aatggagaga tatttactgt tatgaataaa ctttcatatg
    45961 cgacgcatga atataaataa aataatatcg agatatttta tatactgatt tttttattat
    46021 tcgcaaactc agtaataagc tttgctaata tatttcagga aaaccgctta ccctgaatat
    46081 tcagggtaag cgtgagagtt aagaatgaat tacagcggtt gggtttgcgc ttcaaccacg
    46141 gccagcgcca ccatattgac gatacgacgc accgatgcga tcggcgttaa cacgtgaacc
    46201 ggtttcgcca cgcccatcag caccggaccg acagtcacac cttccgagct ggaaacgcgc
    46261 agtaagttgt aactaatgcg ggcagcttcc atgttcggca tcaccagaat attggcagaa
    46321 cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg cttccaccag cgctgcatcg
    46381 ccgtgcattt caccatcaat catcagatct ggcgcacgat ccctgaccag ttccagcgcc
    46441 tgacgcattt tgctcgacga cgggcagtca gaagaaccaa agttggagtg cgacaacaga
    46501 gcaacgcgcg gctcaatacc aaaacgacgg acagtttctg ccgccatcaa ggtgatctcc
    46561 gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat cggcaacaaa ggtgttacca
    46621 ctcggcagca gcagcgcgtt cattgcacct gcggtgtgaa cgccatcgcg ataaccaaag
    46681 acatttttca ccacgctaaa atgttcatga taatcgccca ccgtaccgca gatcattgca
    46741 tcggcttccc cacgctgaac catgatcgcg ccgatcactg tcgggttact gatcagcgca
    46801 cgctgcgcct gttcctgagt gacaccgcga cgcttcatga tctggaagta ttcggtccag
    46861 tactctttaa agcgcggatc ggattcgtta ttgacgattt caaaatcaac gcccgctttg
    46921 atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt tcggacgacc gataaggatc
    46981 ggtttcgcca gtcccagcgt taccagttcc tgagtcgcat gcagaacgcg cgcctcttcc
    47041 ccttccggca gaactacgcg cttcggcgct ttgcgagcct gggagaaaat cggcttcata
    47101 aacaggttgg ttttgtaaac gaactcagtc agcttgtcga tgtagacgtc gaaatcagca
    47161 atcggacgcg tcgccacgcc cgactccatc gccgcttttg cgaccgcagg agcgatctta
    47221 acgatcaagc gcggatcaaa cggttttgga atgatgtatt ccggaccaaa gctcagatcc
    47281 tgaacgccat acgctgaagc caccacttca ctctgttctg catgggccag ttctgcaatc
    47341 gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg ttgcgccaac gtcaagcgcg
    47401 ccacggaaga tgaacgggaa gcaaagaacg ttgttcacct ggttcgggta gtcggaacgg
    47461 ccggtacaga tgatggcatc cggacgcact tctttcgcca gcggcggcag aatttccggt
    47521 tccgggttcg ccagcgccag gatcattggt gcccgagcca ttttcttcac catttcctgg
    47581 gtcagcactt tcgggccgga acagcccagg aaaatatccg caccttcaat cacatcatcg
    47641 agggtacgtt tgccgtcatc caccaccgca tatgcggctt tggtttccgc catatttggc
    47701 tcacgcccct gatagataac gccttttgaa tcgcaaacca cgatgttatg tttttgcagc
    47761 cccagcgcta ccagcaggtt catacaggcg attgctgcgg cacccgcgcc ggaaaccacc
    47821 atccgcacgt cggagatgtt tttctccacc acgcgcaagc cgttgaggat ggcggcagtg
    47881 ctgatgattg ccgtgccgtg ctgatcgtcg tggaataccg gaatattcat ccgctcgcgc
    47941 agtttctgtt caatatagaa acattctggc gctttaatgt cttcgaggtt gatgccgccg
    48001 aaggtcggtt cgagcgcggc gacgacttca ataaatttgt ccgggtcgag ttcatcaact
    48061 tcaatgtcaa atacatcaat cccagcgaat ttcttaaata gaacgccctt gccttccatc
    48121 accggtttgc ctgccagcgc gccaatgttg cctaacccca gcaccgccgt accgttagag
    48181 atcaccgcaa ccaggttacc gcgcgcggta tatttgtagg cttttagcgg gtctttttcg
    48241 atttcaagac aaggtgcggc aacgcctggt gagtaggcca gcgccagatc gcgctgcgtt
    48301 gccagaggct tggttggaga aacctggatt ttccctggaa ctggaaattc atggaaatca
    48361 agtgcacttt gttttaactg gtcatccatt tggttgttcc tttcacgtaa cgttcacaaa
    48421 taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc acattattac cctgccggag
    48481 tctacagact ttgagcaagt ccaaactctc accattaata taatgttttg gtaacaatcc
    48541 tataacactg atgttacctg cttaatccag caataccatg cctgtctgct atgctttttt
    48601 gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc actcatctaa cactttactt
    48661 ttcaaggagt atttcctatg aacgagttag acggcatcaa acagttcacc actgtcgtgg
    48721 cagacagcgg cgatattgag tccattcgcc attatcatcc cctggatgcc accaccaatc
    48781 cttcgctgtt actcaaggct gccggattat cacaatatga gcatttaata gacgatgcta
    48841 tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca ggtggtcgca gcgtgtgaca
    48901 aactggcggt caatttcggt gctgaaattc tcaaaatcgt acccggttgc gtgtcaacag
    48961 aagttgatgc acgcctctct tttgataaag aaaagagtat tgagaaggcg cgccatctgg
    49021 tggacttgta tcagcaacaa ggcgttgaga aatcacgcat tttgatcaaa ctggcttcga
    49081 cctgggaagg aattcgcgcg gccgaagagc tggaaaaaga aggtattaac tgcaacctga
    49141 cgctgctgtt ttcttttgcc caggcgcggg cctgtgcgga agcaggcgtt tttctgattt
    49201 cgccgtttgt agggcgtatt tatgactggt atcaggcacg caagccgatg gacccgtatg
    49261 tggtggaaga agatccgggc gttaaatcgg tgcgcaatat ctacgactac tataagcaac
    49321 accactatga aaccattgtg atgggcgcga gcttccgtcg caccgaacaa atcctcgcct
    49381 taaccggctg cgatcgactg actatcgcac cgaatttgct gaaggagctg caggaaaaag
    49441 tttctccagt ggtacgtaaa ttaatcccgc cttctcagac gttcccacgc ccagcaccca
    49501 tgagcgaagc ggagttccgt tgggagcaca atcaggatgc gatggcggta gaaaaactgt
    49561 ctgaaggcat tcgtctgttc gctgttgatc aacgcaaact ggaagatctt cttgccgcca
    49621 aactataaac cagccacgga gtgttatatg tcccgaaaag accttgccaa tgcgattcgc
    49681 gcactcagta tggatgcggt acaaaaagcc aattctggtc atcccggcgc gccgatgggc
    49741 atggctgata ttgccgaagt gctgtggaac gattttctta aacataatcc taccgaccca
    49801 acctggtatg atcgcgaccg ctttattctt tccaacggtc acgcgtcgat gctgctctac
    49861 agtttgctac atctgaccgg ttacgacctg ccgctggaag aactgaagaa cttccgtcag
    49921 ttgcattcga aaaccccagg ccacccggag attggctata cgccaggcgt tgaaaccacc
    49981 accggcccgc ttggacaagg tttggcgaac gccgtcgggc tggcgatagc agagcgtaca
    50041 ctggcggcgc agtttaacca gccagaccat gagatcgtcg atcacttcac ctatgtgttt
    50101 atgggcgacg gctgcctgat ggaaggtatt tcccacgaag tctgttcgct ggcaggcacg
    50161 ctgggactag gcaagctgat tggtttttac gatcacaacg gtatttccat cgacggtgaa
    50221 acagaaggct ggtttaccga cgatacggca aaacgttttg aagcctatca ctggcatgtg
    50281 atccatgaaa tcgacggtca cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa
    50341 agcgtgaaag ataagccgtc gctgattatc tgccgtacgg tgattggctt tggttcgccg
    50401 aataaagcag gtaaggaaga ggcgcacggc gcaccactgg gggaagaaga agtggcgctg
    50461 gcacggcaaa aactgggctg gcaccatccg ccatttgaga tccctaaaga gatttatcac
    50521 gcctgggatg cccgtgaaaa aggcgaaaaa gcgcagcaga gctggaatga gaagtttgcc
    50581 gcctataaaa aggctcatcc gcaactggca gaagagttta cccgacggat gagcggtggt
    50641 ttaccgaagg actgggagaa aacgactcag aaatatatca atgagttaca ggcaaatccg
    50701 gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta acgcttacgg gccgatgctg
    50761 cctgagttgc tcggcggttc ggcggatctg gctcccagca acctgaccat ctggaaaggt
    50821 tctgtttcgc tgaaggaaga tccggcgggc aactacattc actacggggt gcgtgaattt
    50881 ggcatgaccg ctatcgccaa cggcatcgcg caccacggcg gctttgtgcc gtataccgcg
    50941 acgtgcctga tgtttgttga atacgcccgt aacgccgcgc ggatggcggc actgatgaaa
    51001 gcgcggcaga ttatggttta tacccacgac tcaattggcc tgggcgaaga tggtccgacg
    51061 caccaggctg ttgagcaact ggccagcctg cgcttaacgc caaatttcag cacctggcga
    51121 ccgtgcgatc aggtggaagc ggcggtgggc tggaagctgg cggttgagcg ccacaacgga
    51181 ccgacggcac tgatcctctc aaggcagaat ctggcccagg tggaacgtac gccggatcag
    51241 gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca gcggcggtaa gccagatatt
    51301 attctgattg ccaccggttc agagatggaa attaccctgc aagcggcaga gaaattagca
    51361 ggagaaggtc gcaatgtacg cgtagtttcc ctgccctcga ccgatatttt cgacgcccag
    51421 gatgaggaat atcgggagtc ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa
    51481 gcaggtattg ccgattactg gtacaagtat gttggtctga aaggggcaat tgtcgggatg
    51541 acgggttacg gggaatctgc tccggcggat aagctgttcc cgttctttgg ctttaccgcc
    51601 gagaatattg tggcaaaagc gcataaggtg ctgggagtga aaggtgcctg atggtgatcg
    51661 ccggatgctg tatgttcccc tcaccctaac cctctcccca gaagggcgag gggacggtcc
    51721 gtgcgctttt tagggccgca tctgcgattt tgtttaacgg gtgatccaca acgtgggcct
    51781 ggcgtctggc ccatgccagt tatcgcaggt aggttctgcg gcgtaacgca ccaggcgaaa
    51841 acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt
    51901 cgctaaggtc accagttcac gcgatttctc atcaaatgtt gcgttcatca gttccagttc
    51961 attcgtctcc tgaccgttgt tgaacggcaa acgcaaccga accgggcgcg aggctagcgg
    52021 ctttttacgc gacacaatcc atgccaaatc aatggtgtta taggcccctg cctcacagct
    52081 aatcatcatc agtgctttat catcggtcag cgcagtgaca ttcacctcac gacgtaacgg
    52141 atcaagcgag cagcgcagac cattcatccg ccagtttcca taatccagca aatcgttacg
    52201 ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg
    52261 cgcgggcggt acgctgagag gcggttcgtc ccctttcttg atccacgcgg tttcactgcc
    52321 aacgcgcttt tgctgagcat caataaacaa caacgccgct tttaagccgc tcagagaaat
    52381 ggtctgattg ccgtcgcgta aggtgatcgc cttcccttcc tgaattatct gcaaaaacgc
    52441 ggtgatggtt gccgtatcat cggttactaa taaccatggt gaaatccgcc acttgtcacc
    52501 acttaatgcc aacggctcac catctaatag cagccgtggc gctatctccc cttcagacgc
    52561 atccggcgac ttcaacccac cgcgctcaat acgtaaaacg gcatcggtat gcgccccagc
    52621 gctgcggctc agggtcatca ccagtccatt atgatcgccc gtattgcgcg ccacacaaaa
    52681 attttgatta ttacaggtga cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca
    52741 caccaaagac gtcggcaaca aagcgaaaaa aagagaagga aaatgcgata gcgcatggac
    52801 ggtacgaccc cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg
    52861 cggctcaatc ggatttgtcg gattgagcca ataataaaca acgaaatatt attgctattt
    52921 ttcagtccat taaatgtgac atttgcaaat agttaagcaa taacaccgtc ttaccgtcac
    52981 gtatctcgcc ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa
    53041 tatcttcatc ttcaacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga
    53101 taaaaaagtg gattagctca gtcacaccgc caggcgacat atacagttca aataatttgc
    53161 gcacttcgcc cacttcatag cccgtctctt caatcgcttc tttacgaatg cacacttccg
    53221 gttcgtcgtt atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat
    53281 taacccaggt agcgacacgg aactgacgaa tcagaaccac gctctttttc ttcgcgttgt
    53341 acaggaggat cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt
    53401 caccatcttt gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga
    53461 gaattttgtc tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga
    53521 acaattatat tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact
    53581 gattttaccc ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg
    53641 gtaaccacca gatccgcacc acgcacggcg tcgccaccgg cgaagatttt cggattggtg
    53701 gtctggtaac ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt
    53761 acaccgtgcg actccagcca cggcatcccg tgtggattga agccaaacgc cataatcacc
    53821 gcatcggctg gcatgacaaa ttcactgccc tcaatcggta caggtcgccg acgcccctgg
    53881 gcatccggct cgccaagacg cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca
    53941 ttcagctcaa gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg
    54001 ttcttcacct ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg
    54061 acgttactcg cgccgtggcg cagcgcagta cgcacacagt ccatcgcggt gtcgccgccg
    54121 cccagtacca cgacgttaag tccggcggta ttgataaacg gctcttccgg tagctcttcg
    54181 agccccatca cctgtttagt gttggcaatg aggaacggca gcgcgtcata aacgcccggc
    54241 gtatcttcat tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc
    54301 gcgtcgtatt gttccaaaag cgaatccaaa gagacatctt tacccacttc acaattgagt
    54361 tcgaagtgaa tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc
    54421 agtttgaaag aaggaatgcc gaaagtgagc aagccaccga tttctggatg gcgatcgtac
    54481 accgtcaccg ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccggacct
    54541 gccccgataa tcgccacccg cttgtccact ttggtgacat gacttaagtc aggacgccaa
    54601 cctttcgcca acgcctgatc tgaaatatag cgttcaatgt tgccgatagt taccgcgccg
    54661 tgctcatcgc gaatagtaca ggcaccttca cacaaacggt cttgcggaca aacgcgtccg
    54721 gtaatttccg gcagggtgtt ggtctggtga gaaagctcga cggcggcgtc gatgtttccg
    54781 gctttcacca gttcaatcca ctgcggaata tggttatgca gcgggcaggt ccattcacaa
    54841 acgctgtgct cgccgaactt aaggcagcgc gaggcttccc gttgtgcctg gtcgcagata
    54901 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
    54961 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
    55021 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
    55081 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
    55141 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
    55201 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
    55261 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt gactgattgc
    55321 accattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
    55381 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
    55441 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
    55501 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
    55561 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaaccgg caatgttcat
    55621 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
    55681 gactaaattg cgtttcagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
    55741 gattgttaaa cgccccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
    55801 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
    55861 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
    55921 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcacc
    55981 ggtattaacc aacctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    56041 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    56101 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    56161 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    56221 tttcacgctg gtctttttta ctttgcgccg catacgccat caggtggttg ccccgctgaa
    56281 tcagctggtt accgccagtc agcgtattga acacgggcag ttcgactcgc cgccgctgga
    56341 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaaa tgtcgagcga
    56401 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    56461 cgaggccaag cgtcgtctgg aggtgttgta tcagtgcagt gttcccaggc gctgaacacc
    56521 agccagattg atgtgcattg tttccgccat attttgcaga ttgttcgcga caatgaagca
    56581 gctgaatatc tggagctgaa cgttggggat aactggcgga ttagcgaagg gaagccaaac
    56641 cctgagcagc caatgcagat tttaccggtg acaatgcaag agacggttta cggcgaactg
    56701 cactggcaaa atagtcacgt ttcatcatca gaaccgctgc ttaacagtgt ttcatcgatg
    56761 ctgggacgcg gtttgtactt taatcaggcg cagaagcatt ttcagcagtt attgttgatg
    56821 gaagaacgtg cgaccatcgc ccgcgaattg cacgactcgc tggctcaggt gctttcttac
    56881 ttacgtatcc agttgacgtt actgaagcgt tcgataccgg aagataacgc caccgcacaa
    56941 agtatcatgg ccgatttttc ccaggcgttg aatgatgctt atcggcagtt acgcgagctg
    57001 ttgactactt tccgcctgac gctgcagcag gcggatctcc cctccgcgtt gagggaaatg
    57061 ctggatacgt tacaaaatca aaccagcgct aaactgaccc tcgactgccg tctgccaacc
    57121 ctggcactgg atgcgcaaat gcaggtgcat ttgttgcaaa ttattcgcga agcggggctg
    57181 aatgcgatga agcacgccaa cgccagcgaa atcgccatca gttgcgtcac cgcgccggac
    57241 ggcaatcaca cggtttatat ccgtgataac gggattggta tcggtgaacc gaaagaaccc
    57301 gaaggtcatt atggactgaa tatcatgcgc gaacgcgcgg aacggctggg cgggacgctg
    57361 actttttcac aaccttccgg cggcggcacg ttagtgagta ttagctttcg ctccgcagag
    57421 ggtgagggga aggggaacac gccggctgag gtgtaaataa ttaccctcac accggccctc
    57481 tccctttaaa ggcgagaggg aaaaacgtgc ccatattgac ctgtaagcga gtgtgtcagc
    57541 aatctcatga taatttacat taactccttt ttttctccac gattggctcg taccttgccg
    57601 ctacagtgaa gcaagtcaag cctacaacaa tacgcagaaa cacgaggtcc tcttttaatg
    57661 gcgaatttct ttattgatcg ccccattttt gcctgggtgc tggcaatcct gttgtgtctg
    57721 acaggtaccc tggcgatttt ttcattgccc gttgaacaat accccgatct cgcgccaccg
    57781 aatgtgcgag tgaccgctaa ctatcccggc gcatcggccc agacgctgga aaacaccgtg
    57841 acccaggtta tcgagcaaaa tatgaccggc ctcgataatc tgatgtatat gtcatctcag
    57901 agcagtggca ccggtcaggc atctgtcact ttaagtttta aagcaggcac cgatccggac
    57961 gaagccgtgc agcaagtaca aaaccagctg caatcagcca tgcgaaagtt accgcaggcg
    58021 gtgcaaaatc agggcgtgac ggtgcgtaaa accggcgata ccaacattct gaccattgcc
    58081 ttcgtctcta ccgatggctc gatggataaa caggatattg ctgattatgt tgccagtaat
    58141 attcaggacc cgttaagccg cgtgaatggc gtcggggata tcgatgccta tggttcgcaa
    58201 tattccatgc gtatctggct ggacccggcg aaactcaaca gtttccagat gacggctaaa
    58261 gatgtcactg atgccattga gtcacagaac gcgcagattg cggttgggca acttggtggt
    58321 acaccttccg tcgataagca ggcgctcaac gccaccatta acgcccagtc actgctgcaa
    58381 acaccagaac agttccgcga tatcaccttg cgggtgaatc aggacggctc agaggtaagg
    58441 ctgggcgatg tcgccaccgt cgaaatgggg gcggagaaat acgattatct tagccgcttc
    58501 aatggtaagc cagcctccgg gttgggggta aaactggcct ccggcgcaaa cgaaatggcg
    58561 acagcggagc tggtgctcaa tcgtctcgac gagctggcgc agtatttccc gcacggactg
    58621 gaatacaagg tggcgtatga aaccacctcg tttgttaaag cctccattga agacgtggtg
    58681 aaaacgctgc tggaagctat cgctctggtt ttcctcgtta tgtatctgtt cctgcaaaac
    58741 ttccgcgcca cgctgatacc cactatcgcc gtgccggtgg tgttgatggg aaccttctcc
    58801 gtactttacg ccttcggtta cagcgtcaac accttaacca tgttcgcgat ggtgctggcg
    58861 atcggtctgc tggtggatga cgccatcgtg gtggtggaaa acgtcgaacg tattatgagt
    58921 gaggaaggac tcactcctcg cgaagccaca cgtaaatcga tggggcagat ccagggggca
    58981 ctggttggga ttgcgatggt gctttcggcg gtatttgtac caatggcctt cttcggcggc
    59041 accaccggtg ccatctatcg ccagttctct attaccattg ttgcggcgat ggtgctgtca
    59101 gtactggtag cgatgatcct cactccggct ctgtgtgcca cgctacttaa gccactgaaa
    59161 aaaggtgagc atcacgggca aaaaggtttt tttgcctggt ttaaccagat gtttaaccgc
    59221 aacgccgaac gctacgaaaa aggggtggcg aaaattctcc accgtagcct gcgctggatt
    59281 gtgatttatg tcctgctgct tggcggcatg gtgttcctgt tcctgcgttt gccgacgtcg
    59341 ttcttaccgc tggaagaccg gggcatgttt actacctcgg tacaattgcc cagcggttca
    59401 acccaacaac agaccctgaa agtcgttgag caaatcgaga aatattactt catccatgaa
    59461 aaagacaaca tcatgtcggt gtttgccacc gttggttctg gccctggggg taacgggcaa
    59521 aacgtggcga gaatgtttat ccgcctgaaa gactggagcg aacgcgacag taagaccggc
    59581 acctcgtttg ccattatcga gcgtgcaacg aaggcgttta accaaattaa agaagctcgc
    59641 gttatcgcca gcagcccacc agcaattagc ggtcttggta gttctgcaga ttttgatatg
    59701 gagttgcagg accacgctgg agcgggtcac gatgcgctga tggcagcacg taatcagttg
    59761 ctggcgctgg cggcggaaaa cccggagcta acccgtgtgc gccataacgg cctcgatgac
    59821 agtccgcagt tgcagattga tatcgaccag cgtaaagctc aggcgctggg cgttgatatc
    59881 gacgatatta acgacacact gcaaaccgcc tggggttcga gctatgtgaa tgactttatg
    59941 gatcgcggtc gcgtgaagaa agtctatgtg caggcagctg cgccgtatcg catgctgcca
    60001 gatgacatca atctctggta tgtccgaaat aaagatggcg gcatggtgcc cttctctgct
    60061 ttcgcgacct cacgctggga aacaggctcg ccgcgtctgg aacgctataa cagttattct
    60121 gcggttgaga ttgttgggga agccgcaccg ggggtcagta ccggtacggc gatggatatt
    60181 atggaatcgt tagtgaagca gctgccaaac ggctttggtc tggagtggac ggcgatgtcg
    60241 tatcaggagc ggctttccgg cacgcaggct ccggcgctgt acgccatttc cttgctggtg
    60301 gtattcctgt gtctggcggc attgtatgaa agctggtcgg taccgttctc ggtaatgctg
    60361 gtcgtgccgc tgggggtaat cggcgcgctg ctggcaacct ggatgcgcgg gctggaaaac
    60421 gacgtttact tccaggtggg cctgttaacg gtcattggtt tatcggcgaa aaacgccatc
    60481 ctgatcgtcg agtttgctaa cgagatgaac caaaaaggcc acgacctgtt tgaagcgacg
    60541 ctccacgcct gccgtcagcg tttacgcccg attctgatga cctcgctggc atttatcttc
    60601 ggcgtattgc caatggcaac cagcacgggt gccggttccg gtggtcagca tgcggtgggt
    60661 actggcgtaa tgggcgggat gatttcagct actattctgg ctatttactt cgtgccgctg
    60721 ttctttgtgc tggtgcgccg ccgcttcccg ctgaagccgc gcccggaata agcaataaaa
    60781 aaggcgacat gccaatgtgt cgcctttttc aactttccga ttaagaacct gctcagcggg
    60841 ttcttgctgt ttatactttg tctcaggaat tacttacgaa gcataacttc gataaagtct
    60901 ttccagttcc ccagttcacg ttcaatcata acaacctctc ttataattat gggtattcta
    60961 cggaaaaaat ataccgtggt gaagctaatt tactcgattg ctgcgatgac tacctccggg
    61021 ggacaaacct tatgtaaata ctatggtcct gcggtgataa tttgtatgtg atacacagca
    61081 acatttcgag atattcatac ggcatctaat acttatttaa ttctggttaa aatacagaca
    61141 gataacaaga tgaatattct taatgtttac gttaaaaatg tttaatatta tttaatagtt
    61201 gttaatttga atacttcgat aatgttatat tttctgataa tcatttgcag gcaaaatgtt
    61261 ttcaccctta aatgagtatt tattctcata aatcgaaaaa ggattcatta tggttacact
    61321 ttacggcatc aaaaattgtg acaccattaa aaaggctcgc cgttggctgg aagccaataa
    61381 catcgactat cgttttcatg attaccgcgt cgatgggctg gacagcgaat tattgaacgg
    61441 ttttatcaac gaattaggct gggaagcgtt actcaacacc cgtggtacaa cctggcgtaa
    61501 actggacgaa actacccgca ataaaatcac cgatgcggcc tctgcggcgg cattaatgac
    61561 tgaaatgcct gcaattatca aacgtccatt gctctacgcg cctggtaagc ctatgctgct
    61621 gggtttcagt gattccagtt atcagcaatt tttccatgag gtgtagtcta tgtcgtgccc
    61681 ggttattgag ctgacacaac agcttattcg ccgcccttcc ctgagtcctg atgacgcagg
    61741 atgtcaggct ttgttgattg aacgtttgca ggcgatcggt tttaccgttg aacgcatgga
    61801 ttttgccgat acgcagaatt tttgggcatg gcgtgggcaa ggtgaaacgt tagcctttgc
    61861 cgggcatacc gacgtggtgc cgcctggcga cgccgatcgt tggatcaatc cgccgtttga
    61921 acccaccatt cgtgacggca tgttattcgg gcgcggtgcg gcagatatga aaggctcgct
    61981 ggcggcgatg gtggtagcag cagaacgttt tgtcgcacaa catcccaacc atacggggcg
    62041 actggcattt ctgatcactt ctgatgaaga agccagtgcc cacaacggta cggtaaaagt
    62101 cgtcgaggcg ttaatggcac gtaatgagcg tctcgattac tgcctggtcg gcgaaccgtc
    62161 gagtatcgaa gtggtaggtg atgtggtgaa aaatggtcgt cgcggatcgt taacctgcaa
    62221 cctcaccatt catggcgttc aggggcatgt tgcctaccca catctggctg acaatccggt
    62281 acatcgcgca gcacctttcc ttaatgaatt agtggctatt gagtgggatc agggcaatga
    62341 attcttcccg gcgaccagta tgcagattgc caatattcag gcgggaacgg gcagtaacaa
    62401 tgttattccg ggtgaactgt ttgtgcagtt taacttccgc ttcagcaccg aactgactga
    62461 tgagatgatc aaagcgcagg tacttgccct gcttgaaaaa catcaactgc gctatacggt
    62521 ggattggtgg ctttccgggc agccattttt gaccgcgcgc ggtaaactgg tggatgcggt
    62581 cgttaacgcg gttgagcact ataatgaaat taaaccgcag ctactgacca caggcggaac
    62641 gtccgacggg cgctttattg ctcgcatggg ggcgcaggtg gtggaactcg ggccggtcaa
    62701 tgccactatt cataaaatta atgaatgtgt gaacgctgcc gacctgcagc tacttgcccg
    62761 tatgtatcaa cgtatcatgg aacagctcgt cgcgtgatga gtggttctgc aagaggaaat
    62821 aagcatggac tggctggcta aatattggtg gattctggtg attgtctttt tggtcggcgt
    62881 cctgctgaac gtaattaaag atctcaagcg cgtcgaccat aagaaatttc tcgccaacaa
    62941 gccggagctt cccccgcatc gtgatttcaa cgataagtgg gacgatgacg acgactggcc
    63001 gaaaaaggat caaccgaaga agtaagctga gtgctcaacc gtaggccgga taaggcgtta
    63061 acgccgcatc cggcaatggt gaacgatgcc tgatgcgacg ctcacgcgtc ttatcaggcc
    63121 aggtaatctg tcttttacat catttcaatc acatcatcat cgccaggctt accgccactt
    63181 agcgcttggt cgaaataatg tttcggaatg gtataacgca agtgatccag ggcaaattgc
    63241 atactgcgat tgtcgatcgc atggcctaaa tcctccacga tatccagcgt cacgtcacca
    63301 ccggcactga ttaacgcttc ctgcgccgca actgcatgcg ccagatcgat aaccggatct
    63361 tcaccaccat gaatcaagtg aattgtcgtg gctgtagacg cggtctccgg caggctcgca
    63421 tagcgtccgt taaaggcaat gacgcgcgaa gcaaggcccg gttcggcttt aatgctctcc
    63481 agcgccataa tcgcgccttg tgagaaacca attagcgccg tggcgttagc ccctactccg
    63541 ctctgtttct gccagtagcg taccgtttca ataaacgtcg gcatgatggc atccacacgc
    63601 gcctggcgat tatcttccgt aataccctgc actgaaaacc actgacgccc cgccggatta
    63661 ccgctcggct ccgcgccacc gacactcacc accagcgcat caggaaacag cggtgcaaac
    63721 cagctgccta tttcccccat tgccaccggg ttatccccga caccgtggaa aagcagtaac
    63781 aactgttgtg caggtttatc cgggctttga acaacaaaat ggtcatgttt catggcgaac
    63841 tccttaactg atgtcattaa ttttacgccg ctgactcaca atgaccatgg gagaaaattg
    63901 aaggagtcag tgaaaaaatt gccattgcgt tatgcgatcg cgcagacgct cagtgcgaac
    63961 ctcatttagt gcgaacagcg cctgcgcggc ctcttcccgc tggcgaacca gtaacatctt
    64021 gcggcctgaa agtttaagtg tggtacataa ctgcgcgtca ctggcgtttt tctgtaaacg
    64081 cccacgcagc gccggtaatg ccagttcact agtttgtagc agacgcaata agcaacctaa
    64141 cgatgttaat agcggacgat gagcgaaagc aaaaccggcc agttcaagcc agtcgtcgtc
    64201 agaaaggatg gcatcgttta gtggatcaac aggaagcatt tcgccattcc actgcgcgag
    64261 agcttgcgca tcgcgacgta aacggtaatg ctcacgttca gccagctgtt tacccgcatc
    64321 actcatcggt aacagcgcca tcgccgtata gcaaccgctg ctggcttccc gatgattacc
    64381 catccgcacc agcacaaaac cgcagcgttg ccagaaacgc cataactccc cagtgtaacc
    64441 aaaactcacc gaaagatagt cgaggtcatg cgtatattgc aaagctccag caataagttg
    64501 ccgccctgtg ccttcccgct gacgagccgg atgaactgct atccggctaa cccgccttcc
    64561 gcgcaatgtc gccgccagtg gattgctgcc gtgcgccgcc agcgactggg ccaccagatt
    64621 accccgcggg cgacgaaaac ctgcccatac cgcctgactg agttcttgag ataatccgcc
    64681 ctcatccacc agccacaacg ccccggcaat ctcgttttcg ccagccgcct gtaaaaaatg
    64741 ttgccctggt gcatccatca tccggcgtaa atccagcggc gaggtccggt agtgcgcacc
    64801 agacaataac tgataaacct ttaacggcgt ttctggctcg cttcgccata acgtttgttc
    64861 aaatgcggaa atgacgatat tgccttgtgg tgtatgggtg aagttttcat cgttaaaaac
    64921 cagtgcctca ctaaccattt tttccagcgg gcatccctgt gcccagcgga tcggctgttg
    64981 cagttcaaaa cggtgtaaat gcggaaagcg agcgcaaaat ttcagcaaaa aaccacgtcc
    65041 ggtaccttcg tagccctgca ccgtagtggt taacaacgtt cgaggaaaac gcgataccag
    65101 ttgatgcaac aacggcgcag gtatggctgc ggcttcgtcg accaccagcc agtcggcttg
    65161 ctcatcgctg gctaacaagg catccggtgc aataaagcga aacttctcgc ccgcaaattg
    65221 tgccagtaca tccgttgccg cttttgcggg cgcggtgaca atcgcactgc ccgcaatacg
    65281 agaaatgagt tgccctgcca acgccgactt accgcgccca cgcgcagccg ttaccgctgc
    65341 cacgcccggc ggcatggtca gtagctgctg taagagttgc tgttgttctg gttgtggtgc
    65401 gccggtcgcg gggtgccagt cagtacgggg agtaaaatgc gccaacgaga acggctggtt
    65461 ttgccgccag aggatagcgt cgttatccgc cgtaagtacg cgtttgagat gctggacaaa
    65521 atgcggcgtc gcaatagggt cagggcaatc actccagcgc agcgagtcgg catcaggttg
    65581 gttttcccac tcttcccata cagggagtaa caaaaccagc cagcttcccg ctttcaacgt
    65641 tccgctaagt gctgcaaagg cagcggcatc aaagccgtgg cgggcgtcga ataccgcatg
    65701 ccggaactcg cgcccaagta aagtttgtag cgccgagggt gaacagtggt tttcagcatc
    65761 tagctgcggc gaaatccaca gccagtcgcc aggtaaggca tcacgcaact taagagcatg
    65821 atcaaaacac caaccctctt ctccgctcaa caccagcaag cggcagatcc cttcacgttt
    65881 catttgcgct gttaatgtgt aaagcgcagt cagttcagcc atccctgccc cggaaattaa
    65941 atgcttttac caaaagtatt gcattgtgcc ggatcgccgc tgtcgaaacc acgtttaaac
    66001 cagctgcagc gttgctgaga agtgccatgt gtgaaactgt ctggtactac tcgcccctga
    66061 ctttgctgtt gtaaacggtc atcgccgatg gcctgcgccg cattcagcgc ctcttccaga
    66121 tcgccagttt ccagaacgcc ttgctgctgc atactatgcc cccagacacc ggcaaaacag
    66181 tcggcctgga gttccatacg cacagataag cggtttactt ccgcctgcgt cgcgttttgt
    66241 tgcagttgac gaactttcgg ctcgatgcct aacagtttct gcacatgatg accgacttca
    66301 tgggcgataa cgtacccctg ggcaaaatcg ccatccgcgc caagtttgtc tttcatgtca
    66361 tcatagaagg agagatcgat ataaactgtg ccatccgccg ggcaatagaa cggccccata
    66421 atggactggc ccgccccgca gccggtgcgc gtcattccac ggtacatgac cagcttcggt
    66481 tgctgatagg tcttacccat cttttcgaac tgttgtcccc aggtgtcttc cgtggttgcc
    66541 agaatcaccg aggtgaattt tgcggcttcg tcttcattag ggctaattga ccgcgttgat
    66601 tgttgttgag aaaccggctg cccggtcatc aacccggtta aatcaacgcc atagtagcct
    66661 gcaaccagca cgactatcag taaaatcagc ccgcctttac cgctcggcag acggaaaccg
    66721 ggaccgccca ttgacggacc gccagagctg ttgcgcctgt cttcaacatt gtcactttca
    66781 cgtcgccctt gccaacgcat agatacctca acaatatatt cattatagaa atgatcgtag
    66841 gtggttcaga ggaagattac cacaggaaag cgggatgaaa tggcggagga atgagcgttc
    66901 aatgcatata ttccgagagc ggttgtcttg ccgtgccagc tgcacggcaa gatgatgaac
    66961 agaaaaatca gtccagctgt acacccaggc ggtgggcgac ggcttcatag gcttcgatca
    67021 ggccaccgag actctggcgg aaacggtctt tgtccatttt ctccagtgtt tctttgtccc
    67081 acaggcggct gccgtccggg gagaactcat cgcccagtac cacttcacct ttgtacagac
    67141 caaactccag cttgaagtcg accagaatca gaccagcatc atcgaacagt tttttcagca
    67201 cgtcgttcgc tttgtaggtc agctctttca tacgcgccag gttctctttg ctcacccagc
    67261 caaaggtttc gcagtaagat tcgttgacca tcggatcgtg catggcatcg tttttcagga
    67321 acagatcgaa cagcggcggg ttcagctcaa taccttcttc gattccaaga cgtttcacca
    67381 gagagccagc agcacggtta cgcacgacac actcaaccgg caccatatcc agctttttca
    67441 ccagacattc ggtatcggag agcagacgct ccatttgagt cgggataccc gcttcagcca
    67501 gtttgctcat aatgaagtag ttgaacttgt tgttcaccat acctttgcga tcaaactgct
    67561 caatgcgcgc gccatcccct gctgacgtat cattgcggaa ttcgagcacc aacaggtccg
    67621 ggttttccgt gctgtatacg gttttcgctt taccacgata caactcagct tgcttttgca
    67681 tctttatcac tcctgggtgt gaattaacgt tttaaaatct tttgctgtct ggtgtgccgg
    67741 atgttttttc ggatgcggcg tgaacgcctt atccgaacta cgtccgacaa acaaatttcg
    67801 tgcgaattac cgctaaaatc gcgttttcct gccgacgcac acgtttgcgt atcatatcag
    67861 aaaaaagggc cggatgattc cagccctgta tttttacttg ctaaacgcag cctggaagac
    67921 agctaccagc gcgtcgttct gactctgagt cagagtatga cctttcggat cgatgaactg
    67981 taggctgctg cggttatcta aatcgccaac ctgcagttta tagtcaccgg atgccaggcc
    68041 tggatcgctc gcgcccagtt cctgccagtc gctgtcagac agcggcttat aagttacggc
    68101 catgttgccc tgcgaacggg tgctgtcggt cactttcatg cccacttttt ccagcgacgc
    68161 tggcagacgt tgccaaacca cattgaacgg cccgcgtacg accagcattg gtaaaccggt
    68221 gtcatcagct gcactttgta cgtccatagt ggtggaggca cgattttgcg cagcgttcgc
    68281 ggcgtcagtg gcagatttat ccagaccggc ggaaataacg ttcatcatct ccgtgctgta
    68341 acgctgcatg gaagccgcgt ctgcaaccgg tttgcccgcc tgttccaggt tcagcagttt
    68401 aaccgtaacc gcctgctgat aaccctgcgg cttaacagag atttgataac gaccacgata
    68461 ctgctcgtct tcgtccagac ggttccattg tacccaatcg gtggtcagtg tctgaccagc
    68521 atcatcacgt tgggtgatgg tgtagttttt cgcctgcagc acgctaacca cctgcggcca
    68581 cagagtattg ccacgaccat tttccaccag caatgaagcg gtatcgcccg tgaactgggt
    68641 acgcgcgcca gaaaccagtg ccagcggctg ggctggtgga cgaatgtcca gcgccttacc
    68701 gacagcacca ctaccgttgg tcaccgggat tgcataatca ccggaggtca ccggcaaaat
    68761 cattccagcc ggggcatgaa gctccgcaag cggtgccgct tccaggtagg cttcatcacc
    68821 actgacctga cgcttatagc gtgagtcaga actacaggca gcgagtaata aaacaagcga
    68881 aacacccgca acctttgcca ggcgcgactt ttgaacagag taagccatca aatctcccta
    68941 aactttacag caaaccggca tgcttaagcg ccgctctgac cgtctcacga ccactgtcgg
    69001 tgattggtgt cattggcagg cgcagcgtat cggtcgccac aagacccagt tccttacatg
    69061 cccatttcac cgggattgga ttgggttcga caaatagttt gttgtgtaat ggcatcagac
    69121 gctgattaat aacgcgtgcc tcggcaaaat gcccttctgc tgccagtttg cacatctggg
    69181 ccatatcacg cgctgcgacg ttagccgtaa cggaaataac cccatgaccg cccaattgca
    69241 tgaagtccag cgcgctcgca tcatcgccgc tcagcagaac aaaatcatct gaaaccagct
    69301 ctttgatctg gtttacacgc gttaagttcc ctgttgcctc tttgattccg ataatatttt
    69361 ttactttcgc cagacggccc accgtttccg ggagcagatc gcagccagta cgggacggca
    69421 cattatacag aatttgcggc aggtcagtat gctcagcgat ggctttgaaa tgctgataca
    69481 aaccttcttg cgacggacga ttgtagtaag gggttaccgt caggcagccg acgataccac
    69541 tgtcattgaa gcgctgcgtc aggctaatgg cttccgcagt agcgttagcg ccggtcccgg
    69601 caattaccgg aatgcgccca tcagccagat ccagcgtcat catcaccaca tcagcatgtt
    69661 cgtcatgatt taaggtagcg gactcgccag tggtgccaac agaaacgatc gccgaagtac
    69721 cgctggcgac atgataatca atcagttttt tcaagctagc ccgacagaca ttaccttttt
    69781 catccatcgg agtaacaatc gcgacaatac ttcccgtgaa catgggccat cctctgtgca
    69841 aacaagtgtc tcaatggtac gtttggtatg gcattaaaag caagcagaca gaaccgttct
    69901 gattgttgta tgcatgattt ttttatgctt tccttaagaa caactcaccc cttaaaggaa
    69961 taaccagttt gacactgtca tcgcaacatt atctggtgat cactgcgttg ggtgccgatc
    70021 gccctggaat tgtgaacacc atcacccgtc atgtcagtag ttgcggctgt aatattgaag
    70081 acagtcgcct ggcgatgctg ggagaagagt tcacgtttat catgctgctt tccggttcat
    70141 ggaatgccat cactctgatt gaatcaacgt taccgttgaa aggtgccgaa ctggatcttt
    70201 taatcgtgat gaagcgcacg acggcgcgtc cgcgtccgcc aatgccagca tctgtctggg
    70261 ttcaggtcga tgttgcagac tccccgcatt taattgaacg cttcacagca cttttcgacg
    70321 cgcatcatat gaacattgcg gagctggtgt cgcgcacaca acctgctgaa aatgaacggg
    70381 ctgcgcagtt gcatattcag ataaccgccc acagccccgc atctgcggac gcagcaaata
    70441 ttgagcaagc gttcaaagcc ctatgtacag aactcaatgc acaaggcagt attaacgtcg
    70501 tcaattattc ccaacatgat gaacaggatg gagttaagta atgaatccac tgaaagccgg
    70561 tgatatcgca ccgaaattta gcttgccgga tcaagacgga gaacaagtta atttgaccga
    70621 cttccaggga cagcgtgttc tggtttattt ctacccgaaa gccatgaccc ccggctgtac
    70681 cgtacaggcc tgcggcttac gcgataacat ggatgagttg aaaaaagcgg gcgttgatgt
    70741 gctgggtatc agcaccgata aacccgaaaa actctcccgt tttgcggaaa aagagctgct
    70801 taactttacg ctcctgtctg atgaggacca ccaggtgtgc gaacaattcg gcgtctgggg
    70861 tgaaaagtcc ttcatgggca aaacctacga tggtattcat cgcatcagct tcctgattga
    70921 cgctgatggc aaaatcgaac atgtctttga cgacttcaaa accagcaatc accacgacgt
    70981 tgtgctgaac tggctgaaag aacacgcctg attactttgc tccattccgt gctggctgcg
    71041 cttgcggcca gcatacctca cttctcgtga tcaagatcac attctcgctt tcccctgcga
    71101 cacgggtgtc gaatccattt tttgctgaac gttaatgacc atcatttttg taccgttcag
    71161 aatccagtta atacataact tattgaatag attgagttaa tcagaatggc accctttatg
    71221 caatatgaaa tgcaatgttt catatcattt tcaaggagcc gacatgaacc gctttgtggt
    71281 ggccgaacca ctgtggtgta caggatgtaa tacctgtctc gctgcctgtt cggacgtgca
    71341 taaaacgcaa ggtttacagc aacacccgcg cctggccctg gcgaagacgt caacaatcac
    71401 tgcccctgtc gtgtgtcatc actgtgagga agccccttgc ctgcaggtct gcccggtcaa
    71461 tgccatctct cagagggatg atgcgatcca actcaacgaa agcctctgta ttggctgcaa
    71521 gctttgcgcc gtggtctgcc catttggcgc aatcagcgct tcaggaagcc gtccggtgaa
    71581 tgcccatgcg caatatgttt ttcaggctga aggctcactc aaagacggcg aagaaaacgt
    71641 gccgccccaa catgcgttgc tgcgctggga accgggtgtc cagaccgtcg cggtgaaatg
    71701 cgacctgtgt gatttcttgc cagaaggtcc ggcctgcgtt cgcgcttgcc cgaatcaggc
    71761 gttacggctg atcaccgacg atagcctgca acgtcagatg aaagaaaaac agcgccttgc
    71821 cgcaagctgg tttgccaatg gcggggagga tcccctttcc ctcactcagg agcaacacta
    71881 atggatgccc tgcaattatt aacctggtcg ctgatcctct atctgtttgc cagtctggct
    71941 tcgctgtttt tactcggtct ggacagactg gctattaagc tttccggcat cacatcgctg
    72001 gtgggcggcg tgattggcat catcagcgga attacgcaat tacatgctgg tgtaacttta
    72061 gtcgcccatt ttgccacccc ttttgacttt gccgatttaa ccctgcgaat ggatagcctc
    72121 tcggcattta tggtgctggt tatctccttg ctggtggtgg tttgttcgct ctattcattg
    72181 acttatatgc gcgaatacga gggcaaaggc gcggcggcga tgggcttctt tatgaatctt
    72241 ttcatcgcat cgatggttgc cctgctggtg atggacaacg ctttttggtt catcgtgctg
    72301 tttgaaatga tgtcgctgtc ttcctggttt ctggtcattg ccaggcagga taaaacgtcg
    72361 atcaacgctg gcatgctcta cttttttatc gcccacgccg gatcggtgct gattatgatc
    72421 gccttcttgc tgatggggcg cgaaagcggc agcctcgatt ttgccagttt cgcacgcttt
    72481 cactttctcc ggggctggcg tcggcggtgt ttctgctggc ctttttcggt tttggcgcga
    72541 aagccgggat gatgccgttg cacagctggt tgccgcgcgc tcaccctgcc gcaccatcgc
    72601 acgcttcggc gttgatgtct ggcgtaatgg tcaaaatcgg tattttcggc atcctgaaag
    72661 tagcgatgga tctgctggcg caaacgggtt tgcctctgtg gtggggcatt ctggtgatgg
    72721 cgatcggcgc aatctccgcg ctcctgggcg tgctatatgc gctggcggaa caggatatca
    72781 aacggctgct ggcctggagt accgtcgaaa acgtcggcat tattttgctg gcagtcggtg
    72841 tggcgatgga cggtctgtca ctgcacgacc cgctgctcac cgtggttgga ctgctcggcg
    72901 cactgtttca tctgctcaac catgcgctgt tcaaagggct gctatttctc ggcgcggacg
    72961 cgattatttc gcgtttgcat acccacgaca tggaaaaaat gggggcactg gcgaaacgga
    73021 tgccgtggac agccgcagca tgcctgattg gttgcctcgc gatatcagcc attcctccgc
    73081 tgaatggttt tatcagcgaa tggtacacct ggcagtcgct gttctcacta agtcgtgtgg
    73141 aagccgtagc gctacaactt gcgggtccta ttgctatggt aatgctggca gtcactggtg
    73201 ggctggcagt aatgtgcttc gtcaaaatgt acggtattac tttctgtggt gcgccgcgca
    73261 gtacacacgc tgaagaggca caggaagtgc caaatacgat gatcgtcgcc atgctactgc
    73321 tcgcggcact ctgcgtatta attgcgctta gtgccagttg gctggcaccg aagataatgc
    73381 atattgccca tgcgtttacc aatacccctc ccgtcactgt cgccagcgga atagcacttg
    73441 tacccggcac gtttcataca cgggtcactc cttcattact gttgctgtta ctactggcga
    73501 tgcctttgct gccaggcctt tactggctgt ggtgtcgttc gcgccgcgca gcgtttcgtc
    73561 gcacaggaga tgcctaggca tgcggctacg gctgggaaaa cgcgatggct ccgtcaggca
    73621 atggcgtgat gcagccgctg cgtgtggtct tctctgcgtt atttcgtcta cgacaacagc
    73681 tcgaccctac gctgaggcta aataaaggtc ttgcgcacgt caccgccagg gctcagagca
    73741 cagaaccctt ctgggatgag cgggtgatcc gccccatcgt gagcgccacc caacggctgg
    73801 tcaaagaaat acagcatctg caaagcggcg actttcgtct ctattgcctg tatgtggtcg
    73861 ccgcactggt tgtgctgcta atcgctattg ccgtctaagg aaatcaccat gagacaaact
    73921 ctttgcgacg gatatctggt catttttgcg ttagcacagg ccgtgattct gctgatgcta
    73981 accccacttt ttacgggtat ttcccggcag atacgcgcgc gtatgcactc ccgccgcggg
    74041 ccggggatct ggcaggatta tcgcgatatc cacaaactgt ttaaacgcca ggaagttgcg
    74101 ccgacatctt caggtctgat gttccgcctg atgccgtggg tattaatcag cagcatgctg
    74161 gtgctggcga tggccttacc actatttatt accgtttccc cttttgcggg cggcggcgat
    74221 ctgctcaccc ttatctatct tcttgccctg tttcgttttt tctttgctct ttccgggctg
    74281 gataccggaa gtccgtttgc gggagtcggt gccagtcgcg agttgacgct cggcattctg
    74341 gtcgaaccaa tgcttattct ctcactgctg gtattggcgc tgatagcagg ttccacgcat
    74401 atcgagatga tcagcaatac gctggcgatg ggctggaact cgccgctaac caccgtactg
    74461 gcgttactgg cctgtggttt tgcctgcttc attgagatgg gaaaaattcc ctttgatgtt
    74521 gctgaagcag aacaggaatt acaggaaggc ccgctgaccg aatattccgg tgccgggctg
    74581 gcgctagcga aatgggggca ggggctgaaa caggtcgtga tggcagcact gtttgtggcc
    74641 ctgtttctgc cctttgggcg cgcgcaagaa ctttctctcg cctgcctgct gacttcactt
    74701 gtcgttacgc tgctcaaggt tttgctgatt tttgtactgg cctcaatcgc agaaaacacg
    74761 ctggcacgcg ggcgtttttt actcattcac catgtgacct ggcttggctt cagccttgct
    74821 gcgcttgcat gggtcttctg gttaaccggt ctgtaaggag cactgacgga atatggaaaa
    74881 tcttgctctg acgacgttaa tgctgccttt tatcggcgca ctggtcgttt cgttttcgcc
    74941 acaacgtcgg gccgccgaat ggggggtttt gttcgccgcg ctgaccacgc tgtgcatgtt
    75001 gtcgctgatc tccgcgtttt atcaggccga taaagttgcc gtcacgttga cgttggtcaa
    75061 cgtgggggat gtggcattgt ttggcctggt cattgatcgc gtgagtacgc tgattctgtt
    75121 tgtggtggtg tttctcggtt tgctggtcac gatctactcc acgggttatc tgacggataa
    75181 aaatcgcgaa cacccgcata acggcacgaa tcgttattac gcatttttgc tggtgtttat
    75241 cggcgcgatg gcgggactgg tactctcctc gacgctgctc ggtcagttgt tgttttttga
    75301 aattacgggc ggctgctcct gggcgttgat cagttattac cagagcgata aagcgcagcg
    75361 ttcagcacta aaagcgttgc ttatcaccca tatcggttcg ttggggttgt atcttgccgc
    75421 cgccacgctg tttttgcaga ccggaacgtt tgtgcttagc gcgatgagcg agttacatgg
    75481 cgacgcacgt tatctggttt atggcggcat cctgtttgcc gcgtggggga aatcggccca
    75541 gctaccgatg caagcgtggc taccggacgc aatggaagcg ccaacaccga tcagcgacta
    75601 tctccacgcc gcatcgatgg tgaaagtggg cgtttacatt tttgcccgcg ccattatcga
    75661 cggcggcaat atcccgcatg tgattggcgg cgttggcatg gtcatggcac tggtcaccat
    75721 tctttatggc tttctgatgt atttgccaca gcaggatatg aagcggttgc tggcctggtc
    75781 gaccatcact caacttggct ggatgttctt cggcttgtcg ctctccatct tcggctcgcg
    75841 gctggcgctg gagggtagca tcgcctacat cgtcaaccac gcgttcgcta aaagcctgtt
    75901 tttccttgta gcaggtgcgc tgagttacag ctgcggcacg cgcttgttgc cgcgtctgcg
    75961 tggcgtattg cacaccctgc cgttgccagg cgtgggtttc tgcgtggcag cgctggcaat
    76021 taccggtgtg ccgccgttca acggcttctt cagtaaattc ccgctgtttg ccgccggttt
    76081 tgcgttgtca gtggagtact ggatcctgct gcccgccatg attctgctga tgattgaatc
    76141 ggtcgccagt ttcgcctggt ttattcgctg gtttggtcgc gtcgtgccgg gcaaaccgag
    76201 cgaggccgtc gccgatgccg caccgctgcc agggtcaatg cgcctggtgt tgattgtact
    76261 gattgtgatg tcgctgattt ccagcgtaat cgccgcgacc tggttgcagt aaggagatga
    76321 tgaatgaccg gttctatgat cgtaaataat ctggcgggac tgatgatgct gacatcgctg
    76381 tttgtgatta gcgtcaaaag ctatcgcctg tcatgcggat tttaggcctg ccagtcgctg
    76441 gtgctggtgt ctattttcgc cactctctcg tgcctgttcg ccgcagagca actgctgatc
    76501 tggtccgcca gcgcctttat caccaaagtg ctgctggtac cgttaatcat gacttacgct
    76561 gcacgaaata ttccccagaa catcccggaa aaagcgttat tcggtccggc aatgatggca
    76621 ctgctcgcgg cgttaattgt cctgctttgc gcatttgtcg ttcagcccgt gaagctaccg
    76681 atggctaccg ggctgaaacc ggcgctggcg gtagcgttag gtcattttct gcttggcctg
    76741 ctgtgcattg tcagccagcg caatatcctg cggcaaattt ttggttactg cctgatggaa
    76801 aacggctccc atctggtgct ggcgcttctt gcctggcgag caccggaact ggtggaaata
    76861 ggtatcgcta ccgacgccat cttcgccgtc attgtgatgg tgttactggc aagaaaaata
    76921 tggcgtaccc acggcacgct ggacgtgaac aacttgaccg cgctgaaggg ataatgagat
    76981 gagttattct gtgatgttcg ctttactcct gctcacgccg ctgctttttt cgctgctctg
    77041 ttttgcctgc cggaaacggg ggctctctgc gacttgtacg gtgactgtat tacatagctt
    77101 agggatcaca ctgctgctga ttctggcact ctgggtggtc caaactgccg ctgatgcagg
    77161 agaaatattc gctgcgggac tgtggcttca tattgatggt ctgggcggtt tgttcctcgc
    77221 cattcttggt gtgattggct ttctcaccgg tgtttactcg attggctaca tgcgtcatga
    77281 agtggcacac ggcgagcttt cacccgttac actgtgcgat tactacggtt tcttccatct
    77341 gtttttgttc accatgctgc tggttgttac cagcaataac ctgattgtga tgtgggcggc
    77401 gatcgaagcc accaccttaa gctcggcgtt tctggtaggc atttacggtc agcgttcatc
    77461 gctggaatca gcatggaagt acatcattat ttgtactgtt ggtgtcgctt ttggtctgtt
    77521 cggtaccgtg ctggtatacg ccaacgccgc cagcgttatg ccgcaggcag aaatggcgat
    77581 attctggagc gaggttctta agcaatcgtc cttgcttgac ccaacattaa tgctgttggc
    77641 ctttgtgttt gtgctaattg gctttggtac caaaaccggg ctatttccca tgcacgcctg
    77701 gctgccggat gctcacagtg aagcgccgag tccggtcagc gccctgctct ccgccgtatt
    77761 gctgaactgc gcgctgttgg tgctgattcg ctattacatc attatttgcc aggccatcgg
    77821 cagcgatttc cccaaccggt tgttgctcat cttcggcatg ttgtcggttg ccgtggcggc
    77881 atttttcatt ctggtacagc gggacattaa gcgtctgctg gcgtactcca gcgtggagaa
    77941 catggggctg gtcgcggtgg cgctaggcat tggcgggccg ctgggaattt ttgccgcgct
    78001 gctgcacacc ttaaaccaca gtctggcaaa aacgctgctg ttctgcggtt ccggcaatgt
    78061 actgctcaag tacggcacgc gcgatctcaa cgtcgtctgt gggatgctca aaatcatgcc
    78121 atttaccgcc gtgctgtttg gcggcggtgc gctggcgctg gcagggatgc cgcccttcaa
    78181 catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc acctgctgat
    78241 tatcgtcctg ctgttattgc tgttaacgct ggtgctcgcg ggcctggtac ggatggctgc
    78301 gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg gctggttgac
    78361 cacctcgcca atggtgattc tgctagtcat gatgctggcg atgggaacgc atattccaca
    78421 acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga cgcacgacct
    78481 gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat ctgtttcgga
    78541 gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc gccgccctga
    78601 aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa caggtcacca
    78661 ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat caacatgatg
    78721 gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat tacgcggttt
    78781 attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag gcactggtcg
    78841 atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg gtctggggcg
    78901 agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat cagcgtcgcc
    78961 tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat gcgatggatt
    79021 atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc aatgagggca
    79081 acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat gaaccgggtc
    79141 acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc ctgttttatg
    79201 tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa gtgaccttct
    79261 tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat accaactcgg
    79321 ttgaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc tcgattctgc
    79381 tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc cattttgttg
    79441 gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg acgatggcgg
    79501 aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt gttcgccgcg
    79561 atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg cgcgccgacg
    79621 tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc actcagggca
    79681 ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg ctgatccgcg
    79741 cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg gcaatattcc
    79801 aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg tccgtgtcaa
    79861 agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc cggatacccc
    79921 actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct ttgttgaagc
    79981 gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat tgttccgctg
    80041 gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc tgcgcggcaa
    80101 taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact cctgtaccga
    80161 ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt ataaagagat
    80221 cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga agatgctgaa
    80281 gttactgaaa actattatgc gcgccggaac cccgacggtg aaatatccct tcgcgccact
    80341 ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat gtattgcctg
    80401 cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg acgaccagca
    80461 aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac gttgtgaaga
    80521 agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg tcacccataa
    80581 agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt gcgaacgccc
    80641 gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc aacaaaatgc
    80701 tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt gcaaacaacg
    80761 cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc agctatgagt
    80821 ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac gcaaaagatg
    80881 aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat cgattgcggc
    80941 ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt cgacgcagaa
    81001 cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt atttactggc
    81061 gcagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc ccccgatcat
    81121 aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca cgatctctac
    81181 agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc cggctgcccg
    81241 ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct gcaacagaag
    81301 attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt gtggccgcag
    81361 atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc gggctatcgt
    81421 caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc tacaggaaat
    81481 cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat cgttcagcaa
    81541 ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg ttttctggac
    81601 gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct ctcaggtaat
    81661 gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga atgtcgcctt
    81721 ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg agcaagcccg
    81781 acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca gccacaccgc
    81841 cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt ggcaagcgcg
    81901 tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta tctatctgat
    81961 ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat ggctatgtca
    82021 gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa cggaatgctc
    82081 gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt catcccgctg
    82141 ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag caacttctat
    82201 taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca gggcgagatg
    82261 gtcgattatt cgcgtcacgc cgtccttctc ggcagtagtt gaatggcgga gctacgcttt
    82321 attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa ctggctggca
    82381 cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagaacagca ggtggcgctg
    82441 cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac taatgcagtg
    82501 ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat ccatcatttt
    82561 ttcggtctgg cttcagtcag tatggtactg ggcgatcatc gaaagaacga gaagttcagc
    82621 ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa tatgcctggc
    82681 gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac gcaccgtgca
    82741 gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc taacggctgc
    82801 gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc attgttgctg
    82861 gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca acacatagcc
    82921 gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac cgatttgcag
    82981 gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa tctgggcgtc
    83041 ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt agatattgtg
    83101 gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa agaggtgatc
    83161 gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa aatcaactgc
    83221 gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa aggggcgttt
    83281 actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg cacgttgttt
    83341 ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg cgtattgcag
    83401 gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt cagagtcatt
    83461 gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg cagcgatctc
    83521 ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg tccggaagat
    83581 atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa tcgttcaatt
    83641 gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc gggcaacgtg
    83701 cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa cagtctgaat
    83761 ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga ttcagcgctt
    83821 cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga agaagaacgt
    83881 cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc ccgtggcgcg
    83941 gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg tctgggaatc
    84001 tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa caaactctct
    84061 ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc cgcccataaa
    84121 attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt gtttatggcg
    84181 atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata ggcattaacc
    84241 catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt ttgcggcacc
    84301 agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt tattagttgg
    84361 cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg tattgcctgt
    84421 ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat gtggggagtc
    84481 gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc tgtcagcctc
    84541 ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg cggacgttcg
    84601 ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc cagtggcttt
    84661 gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg ccatttcgcc
    84721 cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggtc
    84781 agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca
    84841 gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc
    84901 ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca tcgggccagg
    84961 cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg ccccagaatc
    85021 cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg
    85081 cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata atcagataca
    85141 ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc acgccaacca
    85201 ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag ccgaccagca
    85261 ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag gtggcgatcc
    85321 ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct
    85381 ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga acggcgttca
    85441 gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc accagataga
    85501 cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca
    85561 tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta
    85621 acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg
    85681 gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca
    85741 tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag cggcgggagc
    85801 agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc acaatagcca
    85861 ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa
    85921 taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca
    85981 tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc
    86041 gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt
    86101 gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga tatttcacag
    86161 tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa
    86221 aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt
    86281 gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt
    86341 ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta
    86401 attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat
    86461 gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc
    86521 tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac
    86581 gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg
    86641 gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg cgcgttaggt
    86701 tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg
    86761 gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc
    86821 attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc gaccttcctg
    86881 gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac
    86941 ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg
    87001 gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat
    87061 tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttctt
    87121 cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac
    87181 gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc
    87241 gatctggcta ccgatatcga tcttgagcaa aacaaagcca atgaggcaat caatcgcctg
    87301 aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc gaacgcttat
    87361 ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat
    87421 aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct aaataaccgc
    87481 gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc
    87541 atttcgctgt tgagtagcgc cagttcgcag gtgaaattag gcagcctgca acaagcgcgt
    87601 tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag
    87661 atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac
    87721 gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg gagccagaag
    87781 tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc
    87841 tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactaa
    87901 acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga
    87961 agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt cgtccaccgg
    88021 agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag cacattgccg
    88081 gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac ttcgcgtagg
    88141 tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag aatttctttc
    88201 acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac
    88261 gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct
    88321 tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca
    88381 tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc gagatccggt
    88441 aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg tgttttgccc
    88501 gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca
    88561 atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc
    88621 ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgcgac
    88681 aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga
    88741 tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag taaagaggag
    88801 ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag
    88861 agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt
    88921 accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt
    88981 gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcacactgat
    89041 cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca taccttttaa
    89101 ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa tcacggaagt
    89161 caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa cacgaatacc
    89221 ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg gcaatgggat
    89281 catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc
    89341 cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc
    89401 gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca ggccgttagc
    89461 aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt
    89521 tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag
    89581 aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc agtgagcatt
    89641 aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc ccaccagcac
    89701 gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac ctaaaaccgt
    89761 gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt gcccttcagc
    89821 cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga tgacggcaac
    89881 gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc ccgctttctt
    89941 cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc ccagcgcgac
    90001 ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc tggaaccaag
    90061 ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa taccgttaaa
    90121 taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg ttgcaccaaa
    90181 catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg gtctttcact
    90241 caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta taggcgcttt
    90301 actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt tatttcgtac
    90361 caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc
    90421 cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc agcgcagcga
    90481 taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc gcttttttca
    90541 gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg
    90601 ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt
    90661 cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt tccagcacac
    90721 cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc ttacctttga
    90781 tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt
    90841 cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct
    90901 tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga
    90961 cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa atcgacggat
    91021 tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca aaatgataac
    91081 agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat tccatccgaa
    91141 aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa aagacgtgca
    91201 aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat tgcgccgaat
    91261 tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata aaacctctct
    91321 tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg gaagaatcaa
    91381 aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc
    91441 gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca ctgacggcgt
    91501 aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg gtattgatct
    91561 ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt ttttcctcga
    91621 ctattacgca accggaaaac tggatgttga taccgcttca gcagtgatca gcggcatcgc
    91681 ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag aaatgccggg
    91741 gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg tagaaaaatc
    91801 agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac tcggttccag
    91861 cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca gcggttgtga
    91921 tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg caccgacccg
    91981 catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca
    92041 cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata atacccaggc
    92101 agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc aaacggcagg
    92161 taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc
    92221 cctacctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg gtgaaaacgc
    92281 gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta tcgaataatg
    92341 aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat tgacgcctgt
    92401 aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc cgacgcgttc
    92461 ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt
    92521 gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta cgcacccgat
    92581 gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc
    92641 gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt acacacccat
    92701 cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt cgtcaccgat
    92761 gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc tggtgatacg
    92821 gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc actggtgatt
    92881 agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa
    92941 cgtctgccgc cgcagagcta cgctgccgac gagtaatgcc cccgtagtta aagcgccagc
    93001 tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt ttttttcccc
    93061 cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca ggcagtgtcc
    93121 cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat cgaaaaagag
    93181 ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa atctaacccg
    93241 ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga gttctataaa
    93301 gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg ctccaacgct
    93361 cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga tcaggaattc
    93421 gacggcctct acaacgagct attgctggag atggcgcgca accagatctt cctgattaat
    93481 gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa gcagtatctg
    93541 cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca gttcctgaaa
    93601 gatgattaca cctatctggc ggtggaaatt atccgtagcg atagcatccg ttacgcgctg
    93661 ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga agcgccgcgt
    93721 cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga tgatattttc
    93781 aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac ccgcgatgcc
    93841 gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc ttccagtctc
    93901 aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat gcccaatgcg
    93961 ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat cgtccccggc
    94021 ggtcgttatc ataattttaa agactttatt aattttccca atgtcggcaa agccaatctg
    94081 gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca gttccgcaat
    94141 ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca cacctttggg
    94201 catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc gattaaaatt
    94261 aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca cgccgcacat
    94321 aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga agaagccaac
    94381 attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc tgcgccgggg
    94441 ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga agtggtccgt
    94501 tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta tactgactat
    94561 tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt taactttatt
    94621 gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca aaactctcgc
    94681 cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg gctgcccagt
    94741 ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg tctgtatgcg
    94801 gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc gctgatcccc
    94861 aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg ttaccttgaa
    94921 catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct ttcttccgcc
    94981 gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct gctcgatccg
    95041 cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac ggtcaaagca
    95101 cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg ccgcaaagta
    95161 cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga ataaccctat
    95221 gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc ttggttcaaa
    95281 cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta ttggccgcct
    95341 gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga gtgaagaggc
    95401 aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag ggttttctcc
    95461 tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg ccactgactt
    95521 tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg gtaatgaaga
    95581 agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc gcaaactggt
    95641 tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg aacctattct
    95701 cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc ctggcggggt
    95761 catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac tggaaacttt
    95821 aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg gtaccataaa
    95881 agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta ccccggaacg
    95941 tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc tgagtttacc
    96001 gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt tatgtggtgt
    96061 gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac gcgaaggcgt
    96121 actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca ccgccagcag
    96181 cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg ataccactat
    96241 gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc aactggaggc
    96301 gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc acagcggttt
    96361 gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta atcaggaaca
    96421 gcagctgatg atggcgacac tgctgcgcta tcaccgtaaa gcgattaagc tcgacgatct
    96481 gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc tattgcgcct
    96541 tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat tgacactgat
    96601 tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc agaatgcgct
    96661 ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg gctggcggtt
    96721 gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg gccgacgaga
    96781 aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat tcagcgtatc
    96841 aattaacggt tgcggcttac caataagata accctgcata taatcgatcc ccaaagagag
    96901 caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca ttttcttcat
    96961 tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg acacaatatt
    97021 gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa gccgtgcata
    97081 gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt cctgaagatg
    97141 ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg tgacttcaaa
    97201 aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt caacaggaaa
    97261 acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag cgggcatctt
    97321 cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt cgatactcga
    97381 cgataaacca aattcgtgcg cgaccggcaa gaagctatcg gggccgatca gttcatcatt
    97441 ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac cggtaatcgg
    97501 ctgggccatc aggaaaaaat ggttgtgctc cagcgcctgc tgtagtcgat tcatcatcgc
    97561 gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca tattttccgg
    97621 ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca gcaggtagat
    97681 atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt gcatcggcat
    97741 cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa tgcgctcctg
    97801 gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat aaacatcttc
    97861 acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgt
//
LOCUS       DADLZM010000002        85902 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLZM010000002 DADLZM010000000
VERSION     DADLZM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.002"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1592)
                     /locus_tag="JNY73_000094"
     CDS             complement(<1..1592)
                     /locus_tag="JNY73_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY8046239.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="JNY73_000095"
     CDS             complement(1576..1932)
                     /locus_tag="JNY73_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY8046240.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSTKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2055..2237)
                     /locus_tag="JNY73_000096"
     CDS             complement(2055..2237)
                     /locus_tag="JNY73_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046241.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="JNY73_000097"
     CDS             complement(2221..2661)
                     /locus_tag="JNY73_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8046242.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="JNY73_000098"
     CDS             complement(2661..2957)
                     /locus_tag="JNY73_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8046243.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="JNY73_000099"
     CDS             complement(2954..3292)
                     /locus_tag="JNY73_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8046244.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="JNY73_000100"
     CDS             complement(3289..4500)
                     /locus_tag="JNY73_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8046245.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="JNY73_000101"
     CDS             complement(4502..5074)
                     /locus_tag="JNY73_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY8046246.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="JNY73_000102"
     CDS             complement(5114..6271)
                     /locus_tag="JNY73_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8046247.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGNTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6574..6735)
                     /locus_tag="JNY73_000103"
     CDS             complement(6574..6735)
                     /locus_tag="JNY73_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY8046248.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(6745..7050)
                     /locus_tag="JNY73_000104"
     CDS             complement(6745..7050)
                     /locus_tag="JNY73_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046249.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7047..7325)
                     /locus_tag="JNY73_000105"
     CDS             complement(7047..7325)
                     /locus_tag="JNY73_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046250.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7605..9353)
                     /locus_tag="JNY73_000106"
     CDS             complement(7605..9353)
                     /locus_tag="JNY73_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY8046251.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9350..9649)
                     /locus_tag="JNY73_000107"
     CDS             complement(9350..9649)
                     /locus_tag="JNY73_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY8046252.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(9655..9888)
                     /locus_tag="JNY73_000108"
     CDS             complement(9655..9888)
                     /locus_tag="JNY73_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046253.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(9890..10110)
                     /locus_tag="JNY73_000109"
                     /pseudo
     CDS             complement(9890..10110)
                     /locus_tag="JNY73_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10103..10324)
                     /locus_tag="JNY73_000110"
     CDS             complement(10103..10324)
                     /locus_tag="JNY73_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046254.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10314..10514)
                     /locus_tag="JNY73_000111"
     CDS             complement(10314..10514)
                     /locus_tag="JNY73_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY8046255.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10523..11269)
                     /locus_tag="JNY73_000112"
     CDS             complement(10523..11269)
                     /locus_tag="JNY73_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046256.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11327..11515)
                     /locus_tag="JNY73_000113"
     CDS             complement(11327..11515)
                     /locus_tag="JNY73_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY8046257.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(11890..13119)
                     /locus_tag="JNY73_000114"
     CDS             complement(11890..13119)
                     /locus_tag="JNY73_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8046258.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13368..14489
                     /locus_tag="JNY73_000115"
     CDS             13368..14489
                     /locus_tag="JNY73_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY8046259.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14538..15764)
                     /gene="pepT"
                     /locus_tag="JNY73_000116"
     CDS             complement(14538..15764)
                     /gene="pepT"
                     /locus_tag="JNY73_000116"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY8046260.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16014..17150
                     /gene="potA"
                     /locus_tag="JNY73_000117"
     CDS             16014..17150
                     /gene="potA"
                     /locus_tag="JNY73_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY8046261.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17134..17991
                     /gene="potB"
                     /locus_tag="JNY73_000118"
     CDS             17134..17991
                     /gene="potB"
                     /locus_tag="JNY73_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY8046262.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            17988..18782
                     /gene="potC"
                     /locus_tag="JNY73_000119"
     CDS             17988..18782
                     /gene="potC"
                     /locus_tag="JNY73_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY8046263.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            18779..19825
                     /gene="potD"
                     /locus_tag="JNY73_000120"
     CDS             18779..19825
                     /gene="potD"
                     /locus_tag="JNY73_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY8046264.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSQLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(19981..20802)
                     /gene="cobB"
                     /locus_tag="JNY73_000121"
     CDS             complement(19981..20802)
                     /gene="cobB"
                     /locus_tag="JNY73_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY8046265.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(20818..21729)
                     /gene="nagK"
                     /locus_tag="JNY73_000122"
     CDS             complement(20818..21729)
                     /gene="nagK"
                     /locus_tag="JNY73_000122"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY8046266.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(21758..23002)
                     /gene="lolE"
                     /locus_tag="JNY73_000123"
     CDS             complement(21758..23002)
                     /gene="lolE"
                     /locus_tag="JNY73_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY8046267.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23002..23703)
                     /gene="lolD"
                     /locus_tag="JNY73_000124"
     CDS             complement(23002..23703)
                     /gene="lolD"
                     /locus_tag="JNY73_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY8046268.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(23696..24895)
                     /gene="lolC"
                     /locus_tag="JNY73_000125"
     CDS             complement(23696..24895)
                     /gene="lolC"
                     /locus_tag="JNY73_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY8046269.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25157..26203
                     /locus_tag="JNY73_000126"
     CDS             25157..26203
                     /locus_tag="JNY73_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY8046270.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSTVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26331..29777
                     /gene="mfd"
                     /locus_tag="JNY73_000127"
     CDS             26331..29777
                     /gene="mfd"
                     /locus_tag="JNY73_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY8046271.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     SERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            29920..30882
                     /gene="ldtC"
                     /locus_tag="JNY73_000128"
                     /pseudo
     CDS             29920..30882
                     /gene="ldtC"
                     /locus_tag="JNY73_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(30965..31222)
                     /gene="bhsA"
                     /locus_tag="JNY73_000129"
     CDS             complement(30965..31222)
                     /gene="bhsA"
                     /locus_tag="JNY73_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY8046272.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31463..32095
                     /gene="comR"
                     /locus_tag="JNY73_000130"
     CDS             31463..32095
                     /gene="comR"
                     /locus_tag="JNY73_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY8046273.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32157..32696)
                     /locus_tag="JNY73_000131"
     CDS             complement(32157..32696)
                     /locus_tag="JNY73_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY8046274.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(32923..34227)
                     /locus_tag="JNY73_000132"
     CDS             complement(32923..34227)
                     /locus_tag="JNY73_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY8046275.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34628..35170)
                     /locus_tag="JNY73_000133"
     CDS             complement(34628..35170)
                     /locus_tag="JNY73_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8046276.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35193..36218)
                     /gene="nagZ"
                     /locus_tag="JNY73_000134"
     CDS             complement(35193..36218)
                     /gene="nagZ"
                     /locus_tag="JNY73_000134"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY8046277.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36229..37053)
                     /gene="thiK"
                     /locus_tag="JNY73_000135"
     CDS             complement(36229..37053)
                     /gene="thiK"
                     /locus_tag="JNY73_000135"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY8046278.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37034..37675)
                     /gene="lpoB"
                     /locus_tag="JNY73_000136"
     CDS             complement(37034..37675)
                     /gene="lpoB"
                     /locus_tag="JNY73_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY8046279.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(37690..38067)
                     /locus_tag="JNY73_000137"
     CDS             complement(37690..38067)
                     /locus_tag="JNY73_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY8046280.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38070..38429)
                     /gene="hinT"
                     /locus_tag="JNY73_000138"
     CDS             complement(38070..38429)
                     /gene="hinT"
                     /locus_tag="JNY73_000138"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY8046281.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            38769..40958
                     /gene="fhuE"
                     /locus_tag="JNY73_000139"
                     /pseudo
     CDS             38769..40958
                     /gene="fhuE"
                     /locus_tag="JNY73_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41018..42451)
                     /gene="ptsG"
                     /locus_tag="JNY73_000140"
     CDS             complement(41018..42451)
                     /gene="ptsG"
                     /locus_tag="JNY73_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY8046282.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(42746..43543)
                     /locus_tag="JNY73_000141"
     CDS             complement(42746..43543)
                     /locus_tag="JNY73_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8046283.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43554..44558)
                     /gene="holB"
                     /locus_tag="JNY73_000142"
     CDS             complement(43554..44558)
                     /gene="holB"
                     /locus_tag="JNY73_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY8046284.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44555..45196)
                     /locus_tag="JNY73_000143"
     CDS             complement(44555..45196)
                     /locus_tag="JNY73_000143"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY8046285.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45186..46208)
                     /gene="yceG"
                     /locus_tag="JNY73_000144"
     CDS             complement(45186..46208)
                     /gene="yceG"
                     /locus_tag="JNY73_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY8046286.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46211..47020)
                     /gene="pabC"
                     /locus_tag="JNY73_000145"
     CDS             complement(46211..47020)
                     /gene="pabC"
                     /locus_tag="JNY73_000145"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY8046287.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47140..48381)
                     /gene="fabF"
                     /locus_tag="JNY73_000146"
     CDS             complement(47140..48381)
                     /gene="fabF"
                     /locus_tag="JNY73_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY8046288.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFSCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48468..48704)
                     /gene="acpP"
                     /locus_tag="JNY73_000147"
     CDS             complement(48468..48704)
                     /gene="acpP"
                     /locus_tag="JNY73_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8046289.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(48915..49649)
                     /gene="fabG"
                     /locus_tag="JNY73_000148"
     CDS             complement(48915..49649)
                     /gene="fabG"
                     /locus_tag="JNY73_000148"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY8046290.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(49662..50591)
                     /gene="fabD"
                     /locus_tag="JNY73_000149"
     CDS             complement(49662..50591)
                     /gene="fabD"
                     /locus_tag="JNY73_000149"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY8046291.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50607..51560)
                     /locus_tag="JNY73_000150"
     CDS             complement(50607..51560)
                     /locus_tag="JNY73_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY8046292.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLGRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51628..52698)
                     /gene="plsX"
                     /locus_tag="JNY73_000151"
     CDS             complement(51628..52698)
                     /gene="plsX"
                     /locus_tag="JNY73_000151"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY8046293.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(52779..52952)
                     /gene="rpmF"
                     /locus_tag="JNY73_000152"
     CDS             complement(52779..52952)
                     /gene="rpmF"
                     /locus_tag="JNY73_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY8046294.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53004..53525)
                     /gene="yceD"
                     /locus_tag="JNY73_000153"
     CDS             complement(53004..53525)
                     /gene="yceD"
                     /locus_tag="JNY73_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY8046295.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            53724..54308
                     /gene="yceF"
                     /locus_tag="JNY73_000154"
     CDS             53724..54308
                     /gene="yceF"
                     /locus_tag="JNY73_000154"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY8046296.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54420..55379)
                     /gene="rluC"
                     /locus_tag="JNY73_000155"
     CDS             complement(54420..55379)
                     /gene="rluC"
                     /locus_tag="JNY73_000155"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY8046297.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            55952..59137
                     /gene="rne"
                     /locus_tag="JNY73_000156"
     CDS             55952..59137
                     /gene="rne"
                     /locus_tag="JNY73_000156"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY8046298.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59334..60287)
                     /gene="flgL"
                     /locus_tag="JNY73_000157"
     CDS             complement(59334..60287)
                     /gene="flgL"
                     /locus_tag="JNY73_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY8046299.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60299..61941)
                     /gene="flgK"
                     /locus_tag="JNY73_000158"
                     /pseudo
     CDS             complement(60299..61941)
                     /gene="flgK"
                     /locus_tag="JNY73_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62007..62948)
                     /gene="flgJ"
                     /locus_tag="JNY73_000159"
     CDS             complement(62007..62948)
                     /gene="flgJ"
                     /locus_tag="JNY73_000159"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY8046300.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(62948..64045)
                     /locus_tag="JNY73_000160"
     CDS             complement(62948..64045)
                     /locus_tag="JNY73_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY8046301.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64057..64755)
                     /gene="flgH"
                     /locus_tag="JNY73_000161"
     CDS             complement(64057..64755)
                     /gene="flgH"
                     /locus_tag="JNY73_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY8046302.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(64808..65590)
                     /gene="flgG"
                     /locus_tag="JNY73_000162"
     CDS             complement(64808..65590)
                     /gene="flgG"
                     /locus_tag="JNY73_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY8046303.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(65728..66479)
                     /locus_tag="JNY73_000163"
                     /pseudo
     CDS             complement(65728..66479)
                     /locus_tag="JNY73_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(66499..68427)
                     /gene="flgD"
                     /locus_tag="JNY73_000164"
                     /pseudo
     CDS             complement(66499..68427)
                     /gene="flgD"
                     /locus_tag="JNY73_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68439..68842)
                     /gene="flgC"
                     /locus_tag="JNY73_000165"
                     /pseudo
     CDS             complement(68439..68842)
                     /gene="flgC"
                     /locus_tag="JNY73_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(68846..69019)
                     /locus_tag="JNY73_000166"
     CDS             complement(68846..69019)
                     /locus_tag="JNY73_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAY8046304.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69174..69833
                     /gene="flgA"
                     /locus_tag="JNY73_000167"
     CDS             69174..69833
                     /gene="flgA"
                     /locus_tag="JNY73_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY8046305.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            69909..70202
                     /gene="flgM"
                     /locus_tag="JNY73_000168"
     CDS             69909..70202
                     /gene="flgM"
                     /locus_tag="JNY73_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY8046306.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            70207..70623
                     /gene="flgN"
                     /locus_tag="JNY73_000169"
     CDS             70207..70623
                     /gene="flgN"
                     /locus_tag="JNY73_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY8046307.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINCSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(70663..72198)
                     /gene="murJ"
                     /locus_tag="JNY73_000170"
     CDS             complement(70663..72198)
                     /gene="murJ"
                     /locus_tag="JNY73_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY8046308.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72365..73231)
                     /locus_tag="JNY73_000171"
                     /pseudo
     CDS             complement(72365..73231)
                     /locus_tag="JNY73_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(73233..73880)
                     /locus_tag="JNY73_000172"
     CDS             complement(73233..73880)
                     /locus_tag="JNY73_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY8046309.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYLLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(73891..74475)
                     /gene="rimJ"
                     /locus_tag="JNY73_000173"
     CDS             complement(73891..74475)
                     /gene="rimJ"
                     /locus_tag="JNY73_000173"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY8046310.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HTCYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            74711..75919
                     /gene="mdtH"
                     /locus_tag="JNY73_000174"
     CDS             74711..75919
                     /gene="mdtH"
                     /locus_tag="JNY73_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY8046311.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            75983..76630
                     /gene="grxB"
                     /locus_tag="JNY73_000175"
     CDS             75983..76630
                     /gene="grxB"
                     /locus_tag="JNY73_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY8046312.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            76764..77324
                     /locus_tag="JNY73_000176"
     CDS             76764..77324
                     /locus_tag="JNY73_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8046313.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77430..78476
                     /gene="pyrC"
                     /locus_tag="JNY73_000177"
     CDS             77430..78476
                     /gene="pyrC"
                     /locus_tag="JNY73_000177"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY8046314.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHTRHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78550..78795
                     /gene="dinI"
                     /locus_tag="JNY73_000178"
     CDS             78550..78795
                     /gene="dinI"
                     /locus_tag="JNY73_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY8046315.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEIPQETWESADDWFVSE"
     gene            79085..79339
                     /gene="bssS"
                     /locus_tag="JNY73_000179"
     CDS             79085..79339
                     /gene="bssS"
                     /locus_tag="JNY73_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY8046316.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79454..80572
                     /gene="solA"
                     /locus_tag="JNY73_000180"
     CDS             79454..80572
                     /gene="solA"
                     /locus_tag="JNY73_000180"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY8046317.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80620..80733
                     /locus_tag="JNY73_000181"
     CDS             80620..80733
                     /locus_tag="JNY73_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY8046318.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            80994..81560
                     /locus_tag="JNY73_000182"
     CDS             80994..81560
                     /locus_tag="JNY73_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY8046319.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81564..82139
                     /locus_tag="JNY73_000183"
     CDS             81564..82139
                     /locus_tag="JNY73_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY8046320.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82181..83233)
                     /locus_tag="JNY73_000184"
     CDS             complement(82181..83233)
                     /locus_tag="JNY73_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY8046321.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83458..84378
                     /locus_tag="JNY73_000185"
     CDS             83458..84378
                     /locus_tag="JNY73_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY8046322.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84550..84747
                     /locus_tag="JNY73_000186"
     CDS             84550..84747
                     /locus_tag="JNY73_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY8046323.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            84771..>85902
                     /locus_tag="JNY73_000187"
     CDS             84771..>85902
                     /locus_tag="JNY73_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8046324.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYWDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20618 a        22857 c        21159 g        21268 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttc gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgtgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgttgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatgg tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgcgtggtgt ggttctgata ttcctactct gctaatttgc tcagccccat
     6601 gtctgcgtaa gtgcgattta ctgcatttct caggtctgcg tagttctccg ttggcggctc
     6661 cggtgtgccc ttgctgctct gttttaccag cgggtcgccc accagccgcc cgtaagctgc
     6721 aatttgtgct gtcatattgc cacctcagtg aagcgatccg gcattgttct ttttcatctc
     6781 ttccatcatt ttttcagtga gcattacgca ctgaaacccc atgtttttga tgtctacggg
     6841 tttcatgcct ttgtcagtgg atatcacagg cagaatatcc tgatacacat cgcggtcagg
     6901 gtaactaagc acacaaagcg cccatgatgc cacgggtgag gatccttcgt ctgtttcaac
     6961 ccaccagcct ggtgtagctg cgcttatttg cagaatcgtg atccgtccgt tgtgcttgta
     7021 aaatttttca ccaaattttg gttcgctcat gattccacct ctccggtttt aacgttgatt
     7081 gttgttacct gttccgcttc ggcaatctcc cgctctgtca gcgtggcaaa atttgccgcc
     7141 gtcgtggtca tgaatgcgct tatcagttcg ggatgtgctt tcgcgtatcc ttcccccgcg
     7201 tggcggtcta tcgttctgat tgccaccttt aaagcgtgct ctgtcatgtc taacgcgcga
     7261 tatttcggtt ctgttctgtc tctgcgtttt ttgagtgatt tattaaattt ccctgaagtg
     7321 tgcatattta tttttacccc ctcgtttaaa aagttttgag ttgtgcctcc ccttgtctac
     7381 cttagtggcc ctcatgccag taatggcgct gctttcagcg gggtagagtg cttttatcca
     7441 ctatctaccc cgtgtctacc tccctgtctg attcaggtaa aatcaggtag agagggtaga
     7501 tagtgggtag acagtaaaaa aaggctacct acctaactta atgcactgaa ttaaatgtat
     7561 ttttctttac tcaggtagac agggtagaca gcaattacaa aaaattataa aaacgcgtcg
     7621 cactcgtctg ttgttattgc gttagtctgc gttactccct taactttccg cgtaatatat
     7681 tcatgtccgt aaactttcgc ggctggcttc atagccttgc caaagtcatt tacgtttagc
     7741 ggtttgctcc tgcctgcgta cgccataaac gccagataga cgcggtaaag gctgtttctg
     7801 gtcgtgtact tcactgaatc gccaccgccg cccatcatca ggccgcgtgc ttcctccaga
     7861 aaattcagga actggcaaaa ctcaataacc ggatccgtct gttgctttat tgccagagct
     7921 tcatcaccgt cacgctgttc cagtagtaaa gcccgtgctt tctcagagtc ggtaaagttc
     7981 gccagcaatc ggcggataat gacagggatt tcagccgcaa tcttttccgg tagctccctg
     8041 tctttttcgg cctcactaac gatattgtcg aaacggaaaa tcacgcgacg acgtgccaca
     8101 cctccggccc gttcggtgaa tatcatcggg ttattgttgg tcgccagcac caccgccctg
     8161 attacagccg tgaaacgctt ttcgtatttc gggttaattt ccacggggtc accgcccgtg
     8221 attttcttga tgcccgttcc ttcgcctgta tatttcggct ggtcagccag gacgataaga
     8281 cgactcccga caacctgcgg acgtccacca gcatcatcaa gcgatgtcat ttcagcgctt
     8341 accgtgttct gtttccctgc cagaaggctg gctatgtgtg tgaatgtact tttaccgctc
     8401 ccgccgtctc cggtggcctc aataaacatc tgccagtcgt accggttcgc cataatcatg
     8461 tacagcgcgg cacatatacg catcatcttg cgctggtctt ttccggctgc gtgctcaagc
     8521 catttatgaa agtttagcgc gttatcgcgg atgttctccc ctggtgctgg tggcgtgtac
     8581 tcaatgccgt tgtgcgtggt gatccagttc tccggcgtgt gcggggaaaa ttcccccgtt
     8641 ttcaggtcaa gcgcaccatt ggcgaacggc agcaaatcgc cggacggctc gcccattggt
     8701 tcggcaataa cttttaacgc ttccacggcg ttattgatta cgcgcttgct gaaagtggcc
     8761 ctgtgctctg aatagatcgc caccatttcg cggctaagtt ccattgtgct gaccggacac
     8821 cataccccgc cgcgccatac gtgaacgatt tcactttcct gatgcacaca aacgccatca
     8881 aagcgctcgg caagcagctg cgcgcgctca ctgtctgcca tttgtgccag ttgcgtattt
     8941 ttctgctccg gcatggtaag ccctgcggca atattttcac gctcagcatt cagatagcgc
     9001 cgccagtttt cacacttctg gacgtgcatt ccttcggggt aaaaatttgc gtcctgaata
     9061 tctgccgccg ccagcttctg accaatcttt ttggtctcca ccaaatccag ttctccggcc
     9121 tggtacagcc ttacgcgttt ttttccatcc ggaacaattt tcagtgcatc aagttcggca
     9181 agctgatttg gcccaagcca cacaggcggc acattatcgc cggatgcggg gccatcctgc
     9241 tcctgccatt gtttcgcgtg cgaccaggca tcactacccg caaagataat tacctctgtg
     9301 tctttgtgtt ttattccgcg tggctgtttt tttacgttcg gtgccagttt cattttttac
     9361 ccctgaatac gttaagcatc ttttttattt cctgaatatt ggcgcgtgct ttctccctgc
     9421 tggttggttt actgcggggc gctgcctgta ccagcgtgaa atcacgccgg aattgataaa
     9481 caggcatcac gcaatcatat tcgtaacctt cacggcggta ggtgatgcgc cgttctgcca
     9541 cgcctttaat cgttaccatg ccgccgtact ggtcgcggta aatatcaccg cgcgtaaatt
     9601 tagggtgagt gttgccactg gcagttaagc cagaatattt aagtttcatt atttttattc
     9661 tccggtgtgc tgttctttat atctgtcgtg caatagatct atttcttgca gttccattat
     9721 tacaggctca agaagcgtta ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc
     9781 attgtcgcca agtgtttcaa gccatatgcg caatatttcc agcatgtttt cactgtgaga
     9841 aagtgcaaga aatgcgcggt ctattgtttc gtggtaaata tcacgcatat taatccccgt
     9901 ccgtcgtttt tcttaaaata acctctgtca cgaaatcagc ataatcggcg gcgatattca
     9961 gtacatcaag cccgttgatt tatattctct cgtggaaagt aagaaaaaag ccgctctaat
    10021 aagttctggc attgacgaaa gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg
    10081 tttcagggaa ttaaaacgat catcacgcat aacccccccc attttcacaa tcggcaacaa
    10141 gaatattttt agcgtcattc agtgaccgcg ttgcgatgta tcggatacat tccagggcga
    10201 actgtgtccg gtctttctgt tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa
    10261 taagcgcgtg catcagcgta gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt
    10321 gcataggttt ccccctgacg aatacgggcg gcgaatacag caacacaacc ggacgggcaa
    10381 cggctacgcg cttcgcgttc cgtccaagcg gttacgtgga tgatttggga ttctccggta
    10441 ctcagtgcca gaaaacgcca cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga
    10501 tttacggcaa ccataacggc tcctagttta cgttgttggt tagaagcccc gttactgctc
    10561 ctacagtgcg gggctttgct ttttgtgttg attgtgtgta atgtgtcatt acacataaat
    10621 acattacatg aggtgtaatt aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc
    10681 cgttccagtt ccgtttagat cctgacttac gggaaaagat ggaaaaggca caacaacagg
    10741 acggcgatga ttctcttgct gcctggataa agcgaataat ccgcaaagaa ttacagcaac
    10801 gcggcataga acccaaagga taaaaactat cagcgccgtg gtgtgaggta ctacggcgca
    10861 ttgctttaca ggcgcacaca atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac
    10921 gaaaaagacg acgcagaaag aaagagcatg aatcagaacg attcgcgccg tgttcttttg
    10981 cccttgagaa gtaccttaaa gaatactcat caaaggagag aacttcgcag ttatggcaac
    11041 gcactgaacc agactgattg cattgcccac cagcctgata gcggctatca tccccgtgct
    11101 tatgtttggg atcacacaca caaacggcgc agcgggttat ctgttcagaa aggcggctcc
    11161 atttcggggc cgcttttttt atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac
    11221 cagggcgaac gaatccccgc ccacgttctg gcgtatattc aatcttcatg gttatagctc
    11281 tgtgttcaga tgattgatgt gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct
    11341 tgcagctatc cttccaggcc agaacctcgg ataaagacca gccaacggaa cgaccgccaa
    11401 gtttacgacg tgatgggaat tgtccggcct tttccaggcg gtagcggcat gagcggctaa
    11461 ggcctgttag cttttcgcat tctttttcac gtataaaccg atcagtgctt aacactattg
    11521 ccccctttcg tttcttaaag agttatttcg tgttctattg cgttgggatg tgtctgattg
    11581 tgtcaggatg attcagagtt ggcaaatgtt gaggccgtat ggtttacaga aagaggaaca
    11641 atcaggataa aatcatttaa attcatgtta atacaaaggc ataaaatatt gtttcatgcc
    11701 ttttttctcg ctatttaaag agtgattcgc tagtgtataa aaaaccagta acacgttaaa
    11761 aatcagctac ttataaatcc gtacactttt tcacctcttg ttcgtagttg ttccgcattg
    11821 ttgctcattg ttgcacgttg tatctgttcg catatccagt atgcgcatac tgaaaaaaca
    11881 cgaaaaaaat tattttcttc tggctactgg tagcgtggtt acgttttcat gtgttcccgc
    11941 cagtatccct aaccgctccg tccacatatc cagcgcattg cgtttagcat ccagataacg
    12001 ggaatgatta taaactcgct gcattcctgg catctggtgg cctgtaagct gctccacgac
    12061 gtgaggatcc acgcctaaat cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag
    12121 tgaccagtga gggtgattta gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt
    12181 ttcctgtttc acttccccca gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt
    12241 gacgaacggc agtattgctt ccggtatggg ccggaatatt gccaccttcg ttttgctgtg
    12301 ttctttcggc acggtccaga gcatttcggt aaaatcccac tcgctgatct ccgataacct
    12361 cagttctacc gtccggcatc cgaacacaat caggaggcgg attaacgcga tgtagtaggg
    12421 ggagaatatt tttttgtcca gtgcctgcaa taattcgccc agttctttgg tgcttaagac
    12481 acgctcgctt atatccggtt ttttcccaac gtccgccaca ctcatatcat caagaacgtt
    12541 gctgattgca tagcgccgcc tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa
    12601 gaatccggca gtaacaggcg ttcgctttgc cacctggtca aaacaggcca gccagtgccg
    12661 tagctcgcat ttatccagcg gcatagcacc aatgtgctgt attacgtgat tattaaggcg
    12721 ctttttcagg gcggcataat ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt
    12781 gagcgcatcg ccaaccgtta ccggctttaa cgcttcctgt acggtgtaat taagctcatg
    12841 acgtggattt ttcccctctg ccagccatgc gcgacactgg gcggcttttt ccctggctga
    12901 tttcaggctc agatcaggat aatttcccag cttaatgcgt tccggtggtg ccccccttcc
    12961 cgttccggcc ctgtaagtga aataccaggt taaaaggcca ctggttgaat gcctgacgct
    13021 caggtttcca ccgtcattaa gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct
    13081 cagctgtgta tcgctcagtt tgttaagtgc tctgctcata attttgattc cggtcatcac
    13141 aattaactac accattaact acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc
    13201 tggaacaagg aaaatcacaa atctgtttat aatcaaaaac ataatgaaca atctgaaaca
    13261 ttatgaaacg gcaaaaaaca ctaattgata aaatatgtcc atacttcacg cattacgtta
    13321 agcatccgtt ataatcggtt gcagatacca gcctgtggat gcttaacatg gaataccaac
    13381 tcactcttaa ctggcccgat tttcttgaac gtcactggca gaaacgcccg gtggtgttaa
    13441 aacgcggctt taataatttt attgacccga tctctccaga cgagttggca ggtctggcga
    13501 tggaaagcga agttgacagt cgactggtca gtcaccagga tggcaaatgg caggtcagcc
    13561 acggcccgtt cgaaagctac gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg
    13621 cagtaaacca ctggcatgag ccgaccgccg cgctgatgcg accgttccgt gaactaccgg
    13681 actggcgtat tgatgatctg atgatttctt tttctgtacc tggcggcggc gtcggcccgc
    13741 atctcgatca gtacgacgtg tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg
    13801 gcgaaaagct gcaaatgaaa cagcactgcc cacatccaga tctgttacag gtcgatccgt
    13861 tcgaagccat catcgatgaa gagctggagc ctggtgatat tctttatatt ccgccaggat
    13921 tcccgcatga aggctacgcg ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc
    13981 caaatacgcg ggaattaatt agcggatttg ccgattatgt gctgcaacgt gaactgggcg
    14041 gcaactacta cagcgatccg gatgttccac ctcgcgctca tcctgcggat gttctgccgc
    14101 aagagatgga taaactgcgt gagatgatgc tcgaattgat caaccagccg gaacacttta
    14161 agcaatggtt tggcgagttt atatcccagt cacgtcatga actggatatc gcgccgccgg
    14221 aaccgcctta tcagccagat gaaatctacg atgcactgaa acaaggtgat gtgctggtgc
    14281 gcctgggtgg tctgcgcgta ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga
    14341 tcgattcccc gcaccgtccg gcactggatg cactcgccag caacattgcg ctgactgcgg
    14401 agaattttgg cgatgcgctg gaagatccgt cattcctcgc gatgctcgcg gcgctggtca
    14461 atagcgggta ttggttcttc gaagggtaag tttggattta agccggatgc agcatcacat
    14521 gccgcatccc ttttcgctta cttccgttgc gccgttaact cggcaatacg gacgatcacc
    14581 tgcaccgctt tttccatacc ttccagaatc acaaactcat gcttaccatg atagttgtaa
    14641 ccgccagtga acaggttcgg gcaaggtaat cccataaacg acaactgcgc gccgttggta
    14701 ccgccgcgga tcggtttcag ttccggttca atatcgcagt cacgcatcgc ctgctgggcg
    14761 atatcgagaa tatgcggatg ctcaaccact ttctcgcgca tattgtagta actgtcttca
    14821 atcaccaatt caatgtagca atcaggatgt aaccctttgc ccactttttt ggcgatctcc
    14881 atcattttac gtttacgcgc ttcaaactgt ttacggtcga aatcacggat gatgtagtgc
    14941 atatcggccc gttcaacggt gcctttcatg ctcgccaggt gatagaaacc ttcatagcct
    15001 tctgtcattt ccgggctttc atccgccgga acttccgcat gaatacgtgc cgccagcgac
    15061 agcgcattta ccatcactcc tttcgccgtg cccggatgaa cattgttacc gacaattttg
    15121 atattgaccg atgcggcgtt gaagttttca aactccagtt cgcctacgcc accaccgtca
    15181 acagtgtaag cccagcgggc atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc
    15241 acttcttcat ccggggtaaa ggcgacgcga atatcaccat gcggaatgtt tttctgttgc
    15301 aataccgcca gcgcggtcat gatttctgca atacctgctt tgtcatcggc acctaacaag
    15361 gttttaccat cggtggtaat caacgtctga cccagtagct gatgcagcac cgggaacata
    15421 accggtgata aaacttcatc gccgataccc agcgcaatat cgccaccgcg atagttttca
    15481 acaatttgcg gattaacatt tttgccgctg caatccggtg aggtatccac atgagaaata
    15541 aagccaatcg ccgggatatc gccagggacg ttagccggta acgtcgccat caaagtgccc
    15601 ttctcactta aggtcacatt gataagcccc atttcttcga gctgctcttt cagcagatgc
    15661 aataacttcc attggccttc cgtgctggga acctgtctca cccctgcttt tgattgggta
    15721 tccagagaca cgtagttcaa aaatcgctca agtagtttat ccatgtagtc accctcactt
    15781 tttgtgacaa cattattaag aagcaagaaa agacaaatat tgcgtcaggt cacttttacc
    15841 cctgcaagcg ggaatattta tcagcattac cttaatgaat ataaagctaa gccagtaatt
    15901 cacaacaaag attggcgatt caagcggttt gccgtagaat taccgccctc attaagagtc
    15961 accaaggtgg ttaaccacaa accccgcatc ggtaagccat ccgttgcgtt tacatgggac
    16021 agagtaaaaa attgaataaa caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa
    16081 ttcgcaaatg ctttgatggt aaagaggtca ttccccagct ggatctgact atcaacaatg
    16141 gcgagttcct cacgctgctt ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga
    16201 ttgcaggtct ggaaactgtt gattccggac gcatcatgct ggataacgag gacatcaccc
    16261 acgttccggc ggaaaaccgc tatgtgaaca ctgttttcca aagctatgca cttttccccc
    16321 acatgaccgt gttcgaaaat gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg
    16381 aaattacgcc ccgcgtgatg gaagccctgc ggatggtgca gctggaaacc ttcgctcaac
    16441 gtaaaccgca tcagctctct ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg
    16501 ttaacaagcc tcgtctgttg ttgctggatg agtcgctctc agcgctggat tacaaactgc
    16561 gtaagcaaat gcagaacgag ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt
    16621 tcgtgactca cgaccaggaa gaagcactca ccatgtcgga caggattgtg gtgatgcgcg
    16681 atggtcgcat tgagcaagac ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt
    16741 ttgttgccgg gttcattggc gaaattaata tgtttaacgc cacggtgatc gagcgactcg
    16801 acgagcagcg cgtacgcgcc aacgttgaag gccgcgaatg taatatctac gttaacttcg
    16861 ccgttgaacc ggggcaaaaa ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag
    16921 agattaacga cgacaaccac gctgaagggc tgattggtta cgttcgtgag cgtaactaca
    16981 aaggcatgac gctggagtcg gttgttgaac tggaaaatgg caagatggtg atggtcagcg
    17041 aattcttcaa tgaagacgat cctgactttg accactctct cgaccaaaaa atggccatta
    17101 attgggtaga aagctgggag gtcgtactgg ctgatgaaga acacaagtaa gttccagaat
    17161 gtagtgattg tcactattgt cggttggctt gtgttgtttg tctttctgcc caacctgatg
    17221 atcattggca ccagcttttt gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg
    17281 ctggataact acacgcgtct gctcgatccg ctctattttg aagtgctatt gcactcgctg
    17341 aatatggcgc tgatcgccac cctcgcctgc ctggtgctgg gctatccatt tgcctggttt
    17401 ctggcgaagc tgccacacaa ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc
    17461 tggaccaact cattgattcg tatctacggg ctgaaaattt tcctcagcac caaaggctat
    17521 ctcaacgagt ttttgctctg gctgggcgtt atcgacacac caatccgtat catgttcacg
    17581 cccagtgcgg tgattatcgg tctggtttac attctgctgc cgtttatggt gatgccgctg
    17641 tactccagta tcgaaaaact ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc
    17701 agcaagttac agacttttat ccgcatcatt attccactga cgatgccggg gattattgcc
    17761 ggatgtctgc tggtgatgct gccagcgatg ggcctgttct atgtgtccga cctgatgggc
    17821 ggtgcgaaaa acctgctgat cggtaacgtc atcaaggtac agttcctcaa tattcgtgac
    17881 tggccgtttg gtgcagccac cagcattacg ctgactatcg taatgggcct gatgttgctg
    17941 gtttactggc gcgcttctcg tttgctgaat aagaaggtgg aactcgaatg atcggtcgac
    18001 tgcttcgcgg cggttttatg accgctatct acgcgtacct gtatatccca atcattattt
    18061 tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa ctggcagggt tttaccacca
    18121 aatggtatag cctgctgatg aacaacgaca gcctgttaca ggcagcgcag cattcactaa
    18181 caatggcggt gttttcggcg acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc
    18241 tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga
    18301 tgtcaccaga tatcgtgatg gcaatttcgc tgctggtgct gtttatgctg ctgggtattc
    18361 agcttggctt ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg
    18421 tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg gcgaaagatc
    18481 tcggtgccag cgaatttacc attctgcgaa aaatcattct gccactggca atgccagcgg
    18541 tggcggcggg ctgggtgtta agctttaccc tgtcgatgga cgatgtggta gtttcttcat
    18601 tcgtcaccgg accaagttat gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg
    18661 gcgtatcgcc ggaagttaac gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg
    18721 ttattgccag ccagcttatt gctcgtgata aaacgaaagg taacacaggg gacgttaaat
    18781 gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg gcactgggca tgagcgccgc
    18841 tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg tgccgccagg
    18901 actgcttgaa cagttcacca aagaaaccgg tattaaggtt atctattcga cttacgagtc
    18961 gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcctatg acctggtggt
    19021 tccttcaacc tattacgtcg ataaaatgcg taaagagggg atgatccaga agatcgacaa
    19081 gtcgaagtta acgaacttca gcaatctcga tccagacatg ctcaacaagc cttttgaccc
    19141 gaataacgac tactccattc cgtatatctg gggtgcgacg gcgattggtg ttaacggtga
    19201 tgcagtggat ccaaaatctg tcaccagctg ggccgatctg tggaagccag agtacaaagg
    19261 cagccagctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc gtaagctggg
    19321 ctactccggt aacaccaccg atccgaaaga gattgaagct gcatataacg agctgaaaaa
    19381 actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt acatggaagg
    19441 cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc aggcgggtac
    19501 gcctattgac gtggtgtggc cgaaagaagg cggcatattc tggatggaca gcctggcgat
    19561 cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc
    19621 ggatgtggca aaacaggttg ctgaaactat cggttatcca acgccaaacc ttgcggcgcg
    19681 taagctgtta agtccagaag tggcaaacga taagacgctc tacccggatg ctgaaaccat
    19741 taaaaatggc gaatggcaga atgacgttgg cgcagccagc agcatttatg aagagtatta
    19801 tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggtattac cgcccgtttt
    19861 ttagttagca aggatgtggc taatgagtca ggacagtaaa gttttatccc gctctttcgt
    19921 cggtattgtg gtccgcttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct
    19981 ttataatccc tccagcaact tttcaacaaa ttctggcacc acctggcttg ccgggccgta
    20041 atatttctcg gcaaattcat taccaacctg actcggttca agattcagtt caacggtgtg
    20101 agcaccatgt agtttggctt cgtgtacaaa cccagcagcc ggatagacat gcccggaagt
    20161 accaatggca ataaagacat ccgccatcga caacgccata taaatttcat ccatgccgag
    20221 tggcatttcg ccaaaccaaa cgacgtgcgg acgcaagggt gccgggaact ggcaacaatg
    20281 gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac tttgcgaaca
    20341 acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg cgcgttcatg
    20401 caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt
    20461 agccagcgca agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg
    20521 ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac
    20581 atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga
    20641 ttccgcagaa attcctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg
    20701 caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt
    20761 tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta
    20821 atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg
    20881 ttcaatgcgc ggaacacgag ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg
    20941 cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac
    21001 aatggtcagg atatttccca gagaaaccgc taataaatcc agataacgct caacgtgcgc
    21061 ccttgcctgc tcatcgcctt gatcataaag cgcaataatt tcgggagcct gcaacggttg
    21121 atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca
    21181 gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca tggttaacgc
    21241 atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac cagtaatcgg
    21301 tttgccgttg aaaaccagcc cgccgccaac gccggtgccg agaattaacc ccatcaccag
    21361 tggatattgc gtaaattcat cctcccaggc ttctgaaagg gcaaaacagt tggcatcgtt
    21421 atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct
    21481 ggcagcgggg acattagcgg catacagctt accatcttct gtttccggca ttcccggaat
    21541 accgatgccg acagaacctt tacagccaaa acgttggtca gcttcagcta ccagctcaca
    21601 cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg
    21661 caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc
    21721 gtaatacatt gcctctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg
    21781 cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca
    21841 atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa
    21901 taaaatagat atcgctggag aggaactgat gaccgatcag cttttcaatc cactcaataa
    21961 tcggggtaag ttgcagtgaa acaactacgc cgataaccac cccacacagg ctgccgaaca
    22021 gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca
    22081 gcgttcttaa tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga
    22141 cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga
    22201 tcatctggat atcgcgatac atatagccgt aagtaccaat ccaactttta atataaacat
    22261 agctgttggt cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca
    22321 ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca
    22381 gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt
    22441 gcaaacgcac acgttttggc tgcatcagtt tatgctcggg attcgagttg gggatcatga
    22501 tcgacaccca atcgccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa
    22561 tttgctgttc gcccgcttta aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg
    22621 cgctcagacg ctgttcctgt tgtgggttaa cgcccttcac ctggattgcg cgcagattcg
    22681 ctccactttc caccagaccg gtgaaattga tatacggcgc agcggcggca atacccggca
    22741 ctttctgcac gttatccagt gcttcctgcc agttagtcca tggctgattt accgcttcga
    22801 tttcgccatg cggcaccacc gccagaatgc ggttattcag ttcgcgttca aagccgttca
    22861 tcgcgcttaa gccgacgatc aataccgcca cgccgagggc aatgccaatg gtagaaatga
    22921 cagagattag cgacaccatg ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca
    22981 ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg
    23041 tccatcacgc atttccagtt ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac
    23101 caccaggaaa gcggtgccct gcaagcgatt caactctcca agcaactgga agatgctgtc
    23161 ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc agtaccagac gcggattatt
    23221 gaccagcgca cgggcaatcg ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg
    23281 gtgattcgca cgatgctcca gccccaccgc ttttaacatc tccagtgcac ggctgttgat
    23341 ttcagcgggc tttttcttgc caatcagcag tggcatagcc acgttttcca gggcagtaaa
    23401 atccggcagc aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagttctgc
    23461 tttcgccgct gaagacagtt tgctcatcgg ctgaccgtta aagatcacat cgccggaggt
    23521 tggcgtatcc agcccgccta acaggtgcag caaggtactt ttaccggaac cagagctacc
    23581 gacaattgcc atcatttcac cttcgcccac gctgaaactg acattgtgca gcacatcggt
    23641 ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt
    23701 cataacgtaa agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag
    23761 acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag
    23821 ccaccggcag cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact
    23881 ggctggcaag cagtgcgcca agcgccgctc cgaggattgc accgataatc ccggcgctgg
    23941 ccccttgcac cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga
    24001 tcgcgacttc gccctgcttc tccatcacca tcagccccag cgaggtaata atgttaaacg
    24061 ccgcaacagc gacaatcagg ctcagcagta agcccatcat atttttttcc atgcgtacgg
    24121 cctggaacag ctcgccttta cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt
    24181 tttgctgact taaagagtca actttcagcg gctcatccag ccacaaacgc cagccggtaa
    24241 tattggctgc cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat
    24301 agccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg cgctgacttg
    24361 gaatacgccc catcggcgtg aactggctgg cagatggcac catcacgcgg atttgatcac
    24421 cgcgattgac gcccaactgt gaggcaagtt gttcgccgag gatgacatta tatttccccg
    24481 gctcgaggtc agtttgtttc acattgacca gatacggcgt tagtggatct ttttgcgccg
    24541 ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac
    24601 cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt
    24661 gctgcgggtt aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga
    24721 tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca
    24781 tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatccg
    24841 ctgcacgccc acgcatgtaa cgcaagccaa taaatagagc gacaggttgg tacatgaaat
    24901 ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc
    24961 tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat
    25021 tgccatgttt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag
    25081 acagataccc attacacctc gggtcataac cagcaggatc aaccgcatgt aaattaaaaa
    25141 agagcgtgtt gttctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg
    25201 tatttgtctg gtggtgattt atcactcggt cattaccttt tatccgcata tgaccacttt
    25261 tcagcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc
    25321 cccctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc gctatatcga
    25381 cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct gggtgctggc
    25441 gctttggggc gtggtgcagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    25501 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    25561 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    25621 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    25681 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    25741 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    25801 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    25861 gctggtatcg actgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    25921 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    25981 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    26041 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    26101 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    26161 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    26221 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    26281 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    26341 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    26401 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    26461 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    26521 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    26581 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    26641 gcgtactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    26701 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    26761 aactggacag cgccggttat cgctatgttg accaggtgat ggagcacggc gaatacgcca
    26821 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    26881 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    26941 aggaagtaga agcgatcaat ctgctgcccg cacacgaatt tccgaccgat aaagcggcaa
    27001 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    27061 tttaccagca agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccattgt
    27121 tcttcagcga accactgccg ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    27181 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    27241 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    27301 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    27361 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    27421 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    27481 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    27541 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    27601 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggtgcgtaat ctggcgctga
    27661 tctgcgaaag cgatctgctc agtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    27721 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    27781 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    27841 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    27901 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    27961 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    28021 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    28081 aacacgatcg tgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg
    28141 atcaggcgca ggccattaat gcggtactta acgacatgtg tcagccgctg gcaatggatc
    28201 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    28261 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    28321 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    28381 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    28441 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    28501 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    28561 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    28621 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    28681 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    28741 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    28801 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    28861 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    28921 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    28981 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    29041 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    29101 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    29161 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    29221 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    29281 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    29341 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    29401 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    29461 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    29521 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    29581 gcgggatgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    29641 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    29701 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    29761 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    29821 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    29881 tgtttattac aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt
    29941 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    30001 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    30061 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    30121 ctcgctctgt tacaggctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    30181 ttaacgatcc cgttgccaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    30241 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    30301 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    30361 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    30421 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    30481 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    30541 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    30601 ctctttagct aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    30661 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    30721 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    30781 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    30841 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    30901 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    30961 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    31021 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    31081 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    31141 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    31201 tgagggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagaaa
    31261 ttacttaacg atcgataact aaatgataga tgtgatatgg atcacataca agatattttt
    31321 tataacaatc attaattaat ttaaaaatct ttaaatttca gtcaaataga aagcaattat
    31381 ttttatggga tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac
    31441 agtcaccagg tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg
    31501 tggccgccca aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg
    31561 gcaacacggt tatgaagcga cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc
    31621 gcccacgcta tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg
    31681 ctatatcgat cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt
    31741 ggagtctgcg ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac
    31801 cccggcaggc tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat
    31861 cgccaatacg ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg
    31921 tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt
    31981 ccttaattgt attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa
    32041 actgatgcag attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc
    32101 aaaaaaagcc cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat
    32161 actttgttat tcaaaatcag ttgcccgtta ctatctagcg ggatctgcgt acccggatcg
    32221 cggtccatgc ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc
    32281 attttttctg acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg
    32341 ctttcctgga gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca
    32401 acagtggcga catctttacc gcgaccacca ccaaactgat gccctatcac gccaccagca
    32461 acagcgccga gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc
    32521 actgtgacgt tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca
    32581 gaaacaacct gagcgtattg cgggccccgt tcaaacacgt tcagactggc cactgccgct
    32641 acgcccaacg cagctgcgac accaatcccg atacccgcca acattgattt attcacggga
    32701 acacctcctt ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga
    32761 cagaagatga aaagcgcggg aatgactgga aatacttaag gattcggagg agtctgaagg
    32821 aatgcctgat gcgacgctta acgcgtctta tcaggcctac cgctcgtacc atttgtccag
    32881 ggcggataag gcgtttatgc cacatcagcc agtgtacgtc gattaatgca acttcaaacg
    32941 cggacggata acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc
    33001 atgcagcgca atctgatgca ttcggtatag cgagatatat acaaagcgcg caattcgtcc
    33061 ttcaatcatc attgagccgc gcgtcaggtt acccatcagg ctaccaacgg tggagaagtt
    33121 cgacagcgat accagcgaac catgatcttt atactgataa tttttcagcg gcttaccgtt
    33181 catctgcgcc agaatgttgt tcattgcgca agtcgccatc tggtgtgcag cctgagcacg
    33241 cggcggaaca aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat
    33301 gtctggatcg cgagtggttt gcagcgtcgg ttccaccacc agctggttga tacggttagt
    33361 ttcaagacca ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccataccat
    33421 cagatcagcc tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt
    33481 gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc
    33541 agagatacgt ggcggtaatg caggcaaaat acgttctccc gcttctacca gcgttacgtt
    33601 cagggcttcg ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga tcgcgttgtg
    33661 caattcagcg gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt
    33721 gccatttgcg cccaggttgg cagagtattt caggaacaga ttcagcatct cctggtggaa
    33781 gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc ctggcgtatt
    33841 gaaatcgtta gaggtgctac ccagcgccat gaccagggtg tcataggcga ttttacgttc
    33901 cggaaccaac agttcacctt tctcgtcgcg tagttctgca atagtgattg ttttcgcttc
    33961 acgatcaata tcaatgacgg aacccaactg gaactggaaa ccatgattgc gcgcatgggc
    34021 cagatagctc aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag
    34081 tggtttccac aggtggctgt ggttacgatc gaccagcgta attttggctt ttttcttacg
    34141 tcccagctta tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat
    34201 cacaatcttt ttcaatggcg tagtcaacgt gaccccctta aatttattaa ccaattgtta
    34261 attaatggtt attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt
    34321 cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt
    34381 ttgttgctta aaagataggc aactttaccg ggggagatca aaaaatagcg tttattattt
    34441 gagcaaattc ggaagagtaa tatagggtga agcgtatatc gagggcgttt ggtcgtcctg
    34501 ataagatgcg cagcatcgca tcaggcattg atgctcgact gtcggatgcg gcgtggacgc
    34561 cttatccgac ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg
    34621 agggcattta cccgagggtt ttgaacgctt taatgcgctg taaatgcggg gagatattct
    34681 tgaatttgtg cgtctgctct tcgtcccaga caatctcgta ataatgatgc aactcttcag
    34741 atgtccgctg gctgttaagt gcttcatcat tacgcgacaa aataaccaga caacgatcgc
    34801 gattcttctc gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc
    34861 gatcaatctt gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc
    34921 ggatatcgca aagaaaacca atccgttccg cccagtatcc gcccaggcca acgccgcaaa
    34981 ttagcggacg ctcgtcaacg ttcagttgca acattttgtc cacttcttta agcagatgct
    35041 gcatatcatg tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa
    35101 actgcaattg taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat
    35161 agataatcat cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag
    35221 cgttcatgta actgattcag acgggtgctg atcgctttcc agcgagccga gtccatcagt
    35281 tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt
    35341 gacagattat ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg
    35401 caacccgcat ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggcacct
    35461 tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac ccagttcctg acgcaaaacg
    35521 gttttcagcc agtagggaga accgctcgcc ggacgcggat caacatcact gtagatcaca
    35581 tgcgcaggca taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata
    35641 tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg gtgtttcttt gtgtgagtct
    35701 gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc
    35761 ataccatcaa taaaccggct ggcaattgcc agggcttttt gtggatcggc atgataagaa
    35821 cgctcgccaa tcgccgcgct gatatgcccg acatccagca ctggcgcaaa gctgatatca
    35881 atatccatag cgatcatttc gctggccatc agccaacccg cctcttgcgc cagtttgccg
    35941 ccctcttcca ttcccaacag cgcagcaaag gattgtgctg ccggtaagcg ggtaaaacct
    36001 tcgcgaaaac gctgcacgcg tccaccttcc tgatctaccg ccaccaccag atgattgcgc
    36061 gatgctgcgc ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc
    36121 gtaaagagaa tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc
    36181 agttcgtaac cttcgacatc caacattact ggacccacac tgctctcctt attgttttat
    36241 taatagctgc cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg
    36301 ccagcggtac tcaaaccacc ctcctttgag catcagcagc cagggaaacc atcgcctgac
    36361 ctgacgccat aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg
    36421 ccggtgctgt tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc
    36481 tccagcatac tcccagtcga tgagttttaa ccctgatgcg ctatgcacta aatttccggc
    36541 gtggacatcc atatgcaatg gacttaagcg taaaggtcgt ggttcccgcg ctttgcgcag
    36601 acgttttaac attcgcagcc aacccacagt ccgccgcgcc ggatcgcttt gctgccagta
    36661 cagttccagt aagggcaaca gcgttattcg ccagccaaaa cgtggttgtt gatgtagata
    36721 atacagcaag cctgccagtt cgttggtatc cggcaaatac gtttttaccg cgccgggcag
    36781 atagtcgact accatccagt cacggagata taaatgcggc ttcggtgcaa tgcttgcggg
    36841 tagttgtgat aaagcccgat actggcgcaa gaacgcggac tgcggcgcat caggatcgtg
    36901 cggctgacgc acaacaaaac gctggccctg atgttcaatg agaaaactac cgccactaag
    36961 cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc
    37021 gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg
    37081 tctgcaccag catcagctgc atttgtaggg tcggagcgtt aacgttgcca gaggcgctgg
    37141 agtacagcac gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtgc
    37201 ctaaactgtc ctgcggcgac aaacctaact gttgcttagc catcgacagt tgctgggcgg
    37261 aaaccagggt aaatttcccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg
    37321 cggcattcag cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg
    37381 cagtcacccc gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt
    37441 cataatggcg aatatgcggc gcaggcggtg ccgttcgatc ttcgtgctca attgggcctg
    37501 gctgctgcgg gatcgtcggc accgagggca ctgtggggac aggctgttgt ggctcggctg
    37561 gttgttccgg cgctggtttc acttcttcta ccggtgcagg ttcacgttgc cccacacacc
    37621 cggcgagaaa catcgccagc gcggtaatca aggcgtagcg actcattttt gtcatcaaga
    37681 ttcacccctt tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
    37741 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
    37801 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc
    37861 ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac
    37921 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
    37981 atcattcacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca acaccagaga
    38041 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
    38101 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg
    38161 ttggtattca tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt
    38221 gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct cagctgacac gtcgttcaca
    38281 gtcggaatga ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
    38341 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
    38401 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
    38461 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
    38521 ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
    38581 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgac
    38641 aaatatgaat gcgtatattt ctcatttgca tttgcatttg caagcaaaat tattcgcaca
    38701 ctaaatataa ccgctcatca ccctcctccg gcatgagcct gacgacaaca taaaccaaga
    38761 gatttcaaat gctttcaaca caatttaaca gggataatca atatcaagcc atcaccaaac
    38821 cgtcactact tgccggttgc atagcactgg cactattacc ttctgccgct tttgctgcac
    38881 cagccactga agaaacggtg attgttgagg gttcagccac agctccagat gatggcgaaa
    38941 atgattacag cgtaacgtct acctctgcgg gtaccaaaat gcagatgact caacgtgata
    39001 ttcctcagtc ggtcactatt gttagccagc agcggatgga agatcagcag ttacaaacgc
    39061 tgggcgaagt gatggaaaac acgctgggga tcagcaaaag tcaggcggat tccgatcgtg
    39121 ctctttatta ttcccgcgga ttccagatcg ataactatat ggttgatggt atccccacct
    39181 attttgaatc gcgctggaat ctgggcgacg cactttctga tatggcactg tttgaacgcg
    39241 tagaagtagt gcgtggcgcg acaggactca tgaccgggac gggtaatcca tctgcggcaa
    39301 ttaatatggt tcgaaaacac gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat
    39361 acggtagctg gaacaaagaa cggtatgtgg cggatttaca aagcccactc accgaagacg
    39421 gtaaaatccg cgcgcgaatt gtcggcggct accagaataa cgactcatgg ctggaccgct
    39481 acaatagtga aaagaccttc ttctcgggca ttgtcgatgc tgatttaggc gatcttacga
    39541 cgctttcagc cggttacgaa tatcagcgca ttgatgttaa tagcccaacc tagggcggtt
    39601 taccgcgctg gaatactgat ggcagcagca acagttacga tcgcgcacac agtaccgcac
    39661 ctgactgggc gtacaacgat aaagagatca acaaggtctt tatgaccctg aagcagcggt
    39721 ttgctgatac ctggcaagcg acactgaatg ccacccactc tgaagtcgaa tttgacagca
    39781 aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt gggccataca
    39841 gtaattatgg acctggcttt gatcatgtcg gcggcaccgg ttggaacagt ggcaaacgta
    39901 aagttgatgc gctggatttg ttcgctgacg gtagttatga attgtttggt cgtcagcaca
    39961 atctaatgtt tggtggcagt tacagcaaac aaaacaatcg ttacttcagt tcatgggcca
    40021 acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc ccacaaaccg
    40081 actggtcacc acagagcctg gcgcaggacg ataccacaca tatgaaatcg ttatatgctg
    40141 ccactcgtgt cacccttgcc gatccgctgc atctgatcct cggcgcacgt tataccaact
    40201 ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct tacgctggtc
    40261 tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct attttccagc
    40321 cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt aacaactacg
    40381 agctgggtct gaaatcggac tggatgaata gccgtctgac caccacgtta gccatcttcc
    40441 gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc agcaacggcg
    40501 aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt gaactcaacg
    40561 gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatctt gcagaggata
    40621 acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg ttcaccagct
    40681 atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgttaactgg caaaatcgcg
    40741 tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc
    40801 tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag gggaacgtca
    40861 ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc tacggcgcac
    40921 cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta aaaaaggcag
    40981 ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg atgtactcat
    41041 ccatctcggt tttcaggtta tcggatttag taccgaaaat cgcctgaaca ccagaaccag
    41101 caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact ttagacacat
    41161 cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg ttttctttac
    41221 caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct tttgcatctt
    41281 cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc acgcggaaga
    41341 tggtgtagta aacaatcgca taaccgatac cgacgatcgg gaacagccac agtttgctgc
    41401 tgttaccaga cagaacgatg aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca
    41461 tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca
    41521 ggatcggcgc aacgaacatg aagaagaact cgatcggctc ggtgataccg gtcaggaacg
    41581 aggtcagcgc cgcggagatc ataataccgc ccactttcgc gcggttttct ggtttagcag
    41641 agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg aagccaccag
    41701 acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg tggaaaacct
    41761 gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc cagatgtggt
    41821 gcagaccaaa cggtaccaga caacgttcga tgaaaccgta aatgccaaac gcaactaccg
    41881 ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc ggcggccaaa
    41941 tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc
    42001 gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa cggttaaaca
    42061 tgtacgctgc aatcgcaccg gagataatcc ctccgagtac gccagtatcc gccaagtgtt
    42121 tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt
    42181 taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg ttattggtaa
    42241 agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg
    42301 cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg gaaccgacgc
    42361 ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat ttaccgacct
    42421 tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag tataggtgct
    42481 ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag
    42541 cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg acggctatca
    42601 cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat gtcacaatat
    42661 ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt tttagccatt
    42721 aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt
    42781 gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg gcaacacctt
    42841 tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc tcttttcctc
    42901 gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga tccaggggga
    42961 cataacgcgc agcatcgcac agttgctccg cattacggaa ggtcacaatg ccggaaaagg
    43021 agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct gtaaaacagt
    43081 gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc gtatcggcac
    43141 gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc tggatatgat
    43201 ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa tccagcccgg
    43261 tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct tctacatcgt
    43321 agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc
    43381 ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc agacaaaatt
    43441 tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc aaagattcat
    43501 aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga
    43561 tgaggaaccg gtagcacaac gcccggttgc aggtaatgct caatacgcag taaaagatcg
    43621 gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg aatgtggcaa
    43681 acatccccca gtatagcctg caggcgcgag ggagaaagat ggtttgccag ttcggcgacc
    43741 aggcccggca catcaacatt ggtcacctgc gcagcgccat gatggcgttt tagcgcatcc
    43801 atcagcaacg ttgccaccca gtgtaaacgc gccggagctt gttcatgatt aagggccgcc
    43861 agcagcgaat accagtcgcc cgatggcacg ctatatgcca acgcctgaca caatgtttca
    43921 cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgccaggcga accggcgctt
    43981 aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga aagccaagtc
    44041 acggcgtact gttctggagg cggcgcaagg taatgtaacc gacaacgact acgtaatgtt
    44101 gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc tggtggctct
    44161 tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc atcggttacc
    44221 cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt gacctcacgt
    44281 accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta gtaatcggga
    44341 tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt gtggccctgc
    44401 ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc gcccatgccc
    44461 ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct ggctaccagt
    44521 ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc ttcacccagt
    44581 gggtcacggt agtgcggatt gcatccatca cggcctccag cggctgggtg gcatcaatgg
    44641 tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga
    44701 aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc
    44761 caacttccgg ggtaacatcg agatagagcg ttaagtcggg gcgaaaatcc ccgagaacag
    44821 catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc tgatacgcct
    44881 gagtggagag atcgtggcga tcgccaatca cccaggtgcc gttagccagc gcaggtttga
    44941 tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg gctttatcgg
    45001 taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac ttttcggcaa
    45061 gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt tgctcgagcg
    45121 tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga
    45181 tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac agacttgtta
    45241 tgactggcaa gattggtatt aaacgtgtga ccacctttac catcggccac aaaatagaga
    45301 tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc
    45361 gcacccggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt ttccaggtct
    45421 gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt tgggtcggtc
    45481 tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg atcgcgttca
    45541 ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg atttttatct
    45601 ttataaggca gaccgtccgc acgcccttcc caggcgctat cgaccgcttt caccattttc
    45661 ttgtgagctc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat caaggtgtct
    45721 ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg cgctacggtg
    45781 gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg
    45841 tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc tttaccgctt
    45901 tccaacaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta agtcccggct
    45961 ttaaagtgag aaagatccgg ttcgatacgc agcagccatt gaaaaacccg tgggcgattg
    46021 atgatcttat cagcataaag ctgttcaccg agcgccagac gtccggtccc tggcttcagg
    46081 gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg aaccttccag
    46141 acgcccacac cagcggcgat acccagtacc accagcaata acaagataat caataacact
    46201 tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat aacgttgctg
    46261 acgaaaaggt gacatcgcca caggcacgta cgggcatcac tggcattaac gcattacaaa
    46321 taaccatctc atctgcctgc aacgcctcct ccagcgaggc ttgcacttcg acaagctgat
    46381 aagaggattg tgccagtaaa cggatacaga attgtcgcat aatgccgtta acacctgcct
    46441 gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta gccgcacagc
    46501 attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa
    46561 gatgagagcg aatcaatact tgctcaagcc gattaagatg tttaatacct gcaagatgag
    46621 gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt
    46681 cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt tgctggtccg ctgttcaatg
    46741 tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca ccattttgct
    46801 gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca caggaaatca
    46861 tcaaccgctg acaagcatcc tgtagtcgct ggatatgcgc cgataacaaa ctgactttac
    46921 cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta
    46981 ctgccagcga ttcctgctta taaccgttaa ttaagaacat accggctcct tatcagagaa
    47041 ttgttagtgt ggcagcatgt tcactacgga acaagtcgga ataaaagcta agaaaaaagg
    47101 cccgcaagcg gaccttttat atgggtggaa aatgacaact tagatctttt taaagatcaa
    47161 agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca ttccgctgac
    47221 ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccgggt tatccaggtt
    47281 gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag attctactgc
    47341 acccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca acacacggct
    47401 tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt
    47461 agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg catcacgcag
    47521 agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg tcatatgata
    47581 agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac cgcgtttttt
    47641 cgcgtgttcg tactcttcaa gtaccagcat accggcacca tcgcccagta cgaagccatc
    47701 acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg tagataatgc
    47761 acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg cgccacctgc
    47821 aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatggccaa tgttgtgcac
    47881 gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat acatgatagt
    47941 cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc tgatcttacg
    48001 tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc
    48061 ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa ggccagaatc
    48121 ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca tcttgcgctg
    48181 ttctttgcgc gagataatgt cctcacagct aaaatccttt actaagccag caaatttcgt
    48241 tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac cggcaagcag
    48301 agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca gtccggtcac
    48361 aactacacga cgcttagaca cgtttgtcct ccaaggaggg aaaaatgatt ctagtgggac
    48421 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
    48481 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
    48541 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
    48601 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
    48661 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
    48721 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
    48781 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
    48841 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
    48901 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    48961 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    49021 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    49081 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    49141 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    49201 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    49261 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    49321 ttagtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    49381 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    49441 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttggca
    49501 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    49561 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    49621 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    49681 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    49741 aggccagtaa gcaccttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    49801 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    49861 cggatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac agaaactgtt
    49921 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    49981 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gccagcagct
    50041 ttacaggcag cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc
    50101 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    50161 gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc ttccggtacg
    50221 gcttcttgca tgaacttacc gcgcatctca accaaacgca ccgcatcagc gaaatcaatc
    50281 acgccagcgc aaaccagcgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    50341 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    50401 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    50461 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    50521 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    50581 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaagtgaat
    50641 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    50701 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc
    50761 accacgacat tgtccatcga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    50821 ttagcctgat gcggaaccag ccagtccagt tgagaacggc caagattatt cgccgccagc
    50881 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    50941 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    51001 tcaccataac taccgtcggc atgcagatgg gtggaaatga ttcccggctc ttcagaggca
    51061 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    51121 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    51181 ttcacgtatt gatcggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg
    51241 tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg
    51301 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    51361 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    51421 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    51481 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    51541 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    51601 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    51661 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    51721 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    51781 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    51841 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    51901 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    51961 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    52021 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    52081 gattgtttta agcaccgctg aggcatcccg aatactgtca agacccttta cttcttcttc
    52141 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    52201 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    52261 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    52321 ctcaatcccc tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    52381 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    52441 tgaactccca cgactggcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    52501 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    52561 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    52621 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    52681 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    52741 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    52801 ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg
    52861 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    52921 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    52981 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    53041 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    53101 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    53161 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    53221 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    53281 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    53341 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    53401 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    53461 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcagggggaa ttttaccttt
    53521 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    53581 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    53641 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    53701 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    53761 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacct gaggtcgacg
    53821 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    53881 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    53941 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    54001 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    54061 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctaagcgagg
    54121 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    54181 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    54241 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    54301 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc
    54361 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    54421 tagcgcgcgt tacgcagttt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    54481 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    54541 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    54601 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    54661 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    54721 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    54781 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc
    54841 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    54901 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    54961 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    55021 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    55081 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    55141 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    55201 agtttatatt caggcttaat acgttttttg ttcacccgca cttcaccttt acgcaaaata
    55261 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt
    55321 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    55381 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    55441 gttttgcacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    55501 cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta
    55561 taacaaggct tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga
    55621 aattttacgg aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg
    55681 tattgatctt tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat
    55741 atttctggag agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa
    55801 aaacgggcaa ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg
    55861 cgactagaca cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cgcagcttag
    55921 tcgtcaatgt aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca
    55981 gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga
    56041 aagtccaggg cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga
    56101 accgagtctg gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact
    56161 aaaagaaatt gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat
    56221 taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg
    56281 caacaaaggc gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat
    56341 gccaaataac ccgcgcgcag gtggcatttc tcgccgtatc gaaggcgatg accgtaccga
    56401 attaaaagaa gcactggcaa gccttgaact gccggaaggc atggggctta tcgtgcgcac
    56461 cgctggcgta ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gcctgaaaca
    56521 ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga
    56581 gagcaacgta atcgttcgcg ccttccgcga ttatttacgt caggatatcg gcgaaatcct
    56641 tatcgataac ccgaaagtcc tcgaactggc acgtcagcat atcgctgcat taggtcgccc
    56701 ggatttcagc agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca
    56761 gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc cgtctgccat ccggtggttc
    56821 cattgttatc gacagcaccg aagcgttaac agccatcgac atcaactccg cacgcgcgac
    56881 ccgcggcggc gatatcgaag aaactgcgtt taacaccaac ctcgaagccg ccgatgagat
    56941 tgctcgtcag ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat
    57001 gacgccagta cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga
    57061 ccgtgcgcgt attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgcca
    57121 gcgcctgagc ccatcactgg gtgaatccag ccatcacgtc tgcccgcgct gctccggtac
    57181 cggtaccgtg cgtgacaacg aatcgctgtc gctctctatt ctgcgtttga tcgaagaaga
    57241 agcgctgaaa gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta
    57301 tctgctgaat gaaaaacgtt ctgcggtgaa tgcgattgaa acgcgtcagg acggtgttcg
    57361 ctgcgtgatt gtgccaaacg atcagatgga aaccccgcac taccacgtgt tgcgcgtgcg
    57421 caaaggggaa gaaacgccga ccttaagcta catgctgccg aagctgcatg aagaagcgat
    57481 ggcgctgccg tctgaagaag agttcgctga acgtaagcgt cctgaacaac ctgcactggc
    57541 aacctttgcc atgccggatg tgccgccagc gccaacccca gctgaacctg ccgcgactgt
    57601 cgtagcccca gcaccgaaag ctgcaacggc aacaccagca gctcctgcac aacctgggct
    57661 gttaagccgc ttcttcggcg cactgaaagc gctgttcagc ggtggtgaag aagccaaacc
    57721 gaccgagcaa ccaacaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa
    57781 acctcgtcag agcaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac
    57841 tgaaggcaac gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcaaac
    57901 tgccgagacg cgtgagagcc gtcagcaggc tgaggtaacg gaaaaagcac gtaccaccga
    57961 cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc
    58021 gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga
    58081 agaacgtgta cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg
    58141 ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc
    58201 tgccgaacca attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct
    58261 gccagtcgta gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga
    58321 caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca
    58381 gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt
    58441 agcgtgcgcg tctccggaac tggcctctgg caaagtctgg atccgctatc caattgtacg
    58501 tccgcaagat gtacaagttg aagagcagcg cgaacaggaa gaagtacaag tgcagccgat
    58561 ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga
    58621 agaaatggct gaagtcgtag aagcccccgt accggttgcc gaaccgcaac cggaagtggt
    58681 tgaaacgacg catcctgaag tgattgccgc cgcggtaact gaacagccgc aggtgattac
    58741 cgagtctgat gttgccgtag cccaggaagt cgcagaacac gcagaaccgg tggttgaacc
    58801 gcaggaagag acggcagata ttgaagaagt tgccgaaact gctgaggttg tggttgctga
    58861 acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga
    58921 aacggtagcc gcggttgaac cagagatcac cgttgagcat aaccacgcta ccgcgccaat
    58981 gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg
    59041 ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc
    59101 ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc
    59161 cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa aattcatcaa
    59221 atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc cccggcgatc
    59281 ccagagcggg cagacaaaac atacccagtt tcatgatatg ttttaaagcg agcttatttg
    59341 ttgagctgga agagcgacaa tccctgcata tcggtaaatg ctttatacga tgcctgcaat
    59401 gccgtttgct gcatgatgta agatgaaata gttgcattcc agtccacatc aaccagatcg
    59461 ctcatctgct gcgtttgccc taaagcgcga tcgctaccta aggaatccag cgactccagt
    59521 ttgttcagct gcgtgcctaa ttccgtgcga acggtaagga cattattcag cgagtttttc
    59581 agtccacggt tggttttatc caatgccgca gcggcgattt ctttatccgc ttcgctatcc
    59641 gcgaccggcg ttttcagggc tgcgatggcg ctatccagca tggcaaaaag attggtttca
    59701 gaagcgctac cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa aattttgtcg
    59761 cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat actttctgtt
    59821 ccgccgacat aatcaccgtt agcctcacta aacggcgcag tctctgtttt ataaccggca
    59881 aaaatgtagc gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc
    59941 ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg
    60001 tagacaattt tttcctgagc attctggata gcagtggtga cctggctaag tacactctct
    60061 tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc
    60121 tgtgcctggg agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg
    60181 actcgcttac ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg
    60241 atgccgcgca tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt
    60301 agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg
    60361 ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg
    60421 aaatcgactg ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg
    60481 ttttcaacgt cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa
    60541 aggatttcgc accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt
    60601 tgcggttgtc gctatcaccc gcatcttctt cgctcgccat cgcgattttc gcttcgtcgg
    60661 tgattaatac atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt
    60721 taacggcagg cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg
    60781 catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat
    60841 tatcgaacga gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac
    60901 cgatcgcaac gtcacctttg tttttcgtgt tttgctgaac cgcgggctta ccgatagtaa
    60961 agaaatcttc accagcatcg ccgttagcat caaatccggc tttgtgttgg ctgttgaaag
    61021 cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag
    61081 aacggaatgt cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa
    61141 tattgcctgc cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag
    61201 gaactgccgc cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggcatcgt
    61261 gatgttataa gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc
    61321 gctcaccagt tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc
    61381 tgtcaggcgc gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg
    61441 atcaacgctg gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc
    61501 ggtggtttta aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgccgc
    61561 cgggtcttcc gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt
    61621 tgccagcgaa gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg
    61681 ggcggtcagg ccgctacttt gcgtctgcac agcacgtaac tggttggtaa taaacgtatc
    61741 atactcacgc tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa
    61801 cgtgctattg gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct
    61861 ggagatatta ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc
    61921 gttattgatc aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg
    61981 gcagcgaccc gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt
    62041 ttgctcacct ggtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca
    62101 tagtgaggat cggtagcata gcccgcgtcc tgtaggacct gcgccccctg ttccgcactc
    62161 gcggcggtcg tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac
    62221 aatgcttcca gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg
    62281 ttttcatatt cagtcgtggt gatttcagtg acttgccctt tccagttgcc agaggctttg
    62341 acaccaaaca ggttatagct tggctcgccg ttttcgcggc ggatttgccg ttgcccccaa
    62401 ccagattcaa gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt
    62461 gccagttggg cgggcagcga gagttgcgcg aggaatgctc tactgtcacc cggcagcgaa
    62521 tcatcgtagt tacgtggtac ggccttttgc accagctgcg aaagcgtctg gttttgataa
    62581 cgcaccacgg tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat
    62641 ggttgttctg gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc
    62701 gtcatctgtt gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg
    62761 ctgaacaggc catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac
    62821 atcccttcca cctgacgggc caccggacgg atatttgccg ccggatcttc gctcgctttc
    62881 gcctttagtt cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg
    62941 ctgatcatca gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca
    63001 aaatagacat cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc accacgttat
    63061 tgaggctggc gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct
    63121 gcgtttgtgg agtaacaaca gtctgtccac caccaaacgg tgtatctggc tggctgacat
    63181 tggcctgacg attaactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg
    63241 tcacttctcg attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct
    63301 gcggggtgac attaacctgc atattctgga tatcggcaag gaaacggacc tgggaactgt
    63361 tgccacttgg tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc
    63421 cacgcacgcg gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa
    63481 gttgcaaatt aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg
    63541 caccattggt gatccgtcca ccgttcagtt ggttaacctg aacactgcta ccgcccgcgg
    63601 aggctcctgc gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa
    63661 cgcccttaag cggtgtcatc aacaacgtac ctccacgcaa gcttttggca ttacccatgg
    63721 aagaaaccac cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca
    63781 ccattaccac agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca
    63841 gctgtgagag catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac
    63901 cagtgccatc cagccccact accaggccat agccaatcag tgagttttgc cttaccccct
    63961 gaacactggt gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa
    64021 ttaatgcaga gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa
    64081 gaaacgctgc aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc
    64141 aatacgcgca tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg
    64201 attaaccacg cccgagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc
    64261 acccaccaca tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc
    64321 gctaaaggta ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc
    64381 ttcgacatcg gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa
    64441 gccaaaatta gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac
    64501 gttctcctgc aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa
    64561 cagcggttga tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac
    64621 gggcgtcgga ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg
    64681 tatccaggcg cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc
    64741 agcgtttttt tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg
    64801 ttatgcctta gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac
    64861 tgttgatttc gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga
    64921 cgttagacgt ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc
    64981 tttcgttcgg tgcaccggag gattgcgttt cggtgtagag gttttcgcca atgctctcca
    65041 gtccggtatc attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct
    65101 ggccttgttg ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg
    65161 ccggaatggt gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct
    65221 gatccacctg gaaagagccg tcacgggtat aggctgatga gccatctggc aacattacct
    65281 ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt attggtctgc gacagattgc
    65341 cctggctgtg taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg
    65401 agggtaaggt ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca
    65461 gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt
    65521 tggcaatgac gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg
    65581 aactgatcat ataatgtcct gtagtggatt gctgacaagc agagcagaag cacgcgacag
    65641 tgtgaatttc ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct
    65701 tttattgcaa tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc
    65761 tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc
    65821 aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat
    65881 cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct
    65941 taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt
    66001 cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt
    66061 gccatcggca gcaatcgtga tttccgcccc ttccggcaca gcaattggcc ccgcctcgcc
    66121 tatcaccgga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt
    66181 acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccatcctg ctgcaacgca
    66241 acgtccagcg ggcgcgaggt gtaatccatc ttgccgggcg tcatgtctgc acccggtgtt
    66301 gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac tgcgcgcaag
    66361 gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg ccagattact ggcggttacc
    66421 gcctgttgat tcagcgtctg gctggctgct cccatcgcag tataaattgc gtgatccatt
    66481 gagctatccc gtcagcgatt agcgtaagtt aacccgcgtg ttgaggatct ggtcctgggt
    66541 tttgatggtc tgggcgttag actgatagtt acgctgggca acgatcatat tgaccagttc
    66601 tttactgaga tcgacgttgg acgcttccag cgcaccgttg gtcagggtgc caaagtttcc
    66661 cgtcccggct gtccccaaca gcgccacgcc agaagattgc gtcgcagacc agacgttgtc
    66721 gccttcggat gccagacctt cgttgttggc aaagttcgcc agtacaatct gccccagcag
    66781 ttgggtttgt tcgttggaat agttgccgac aaccgtaccg tcatcattga tttgataact
    66841 caccagatcg cccggtttgt agccattctg ggtggttgcc acaatgttgt tagcgccggt
    66901 attttgctgc atggagttga ggaagctcag actaaacgtg gcgggttctg caccgttaat
    66961 tgcgccggtt gcgatattat tcgccatcgc accatccact aatgtgccat tagcattaaa
    67021 ttccagtgtt gtcgctgtct tcgcaatgct gtttggatca ctgctatcct gggtgtagac
    67081 ctgccagtta ttatccccgg tcttcacaaa atagacgctc atgtcatgag cattaccctg
    67141 actgtcgaaa acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa
    67201 tggcgtaaca gtaggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt
    67261 ggtagttttc gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg
    67321 ctgaatagtc ggcggcgtac cggttgccgg gtaacccgtc agctgtaaac cttgcatatt
    67381 caacaggtta cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc
    67441 gttgctgtct accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc
    67501 ggtgttggtg gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag
    67561 tcccactttc gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta
    67621 ggtggcggag ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa
    67681 tccgctaacc gcttgagaaa aggccatgac tgactcctga aatgtgaagg ctcaaattat
    67741 ctgccgtact tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct
    67801 gttgccgcgg atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt
    67861 accaccgtta ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccatc
    67921 agtcaacgta ccgtcccagg tgaaactgtg aactccggcg gtcagttcac caatatcaat
    67981 ggtgcgcaca accgcgccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg
    68041 ttgcagctca acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc
    68101 cgcaagaaca gttgtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa
    68161 cgactggctg ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat
    68221 cccgctgacc gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt
    68281 ggtcgggtcc tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc
    68341 tgcggcgttg ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg
    68401 atcggtggtg gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt
    68461 ttcagcatca tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac
    68521 gccgacatgg tgttaaccat ctccccgacg acatcaacgt tcggcatttt tacgtagccc
    68581 tttgcatctg ccagcggatt acctggttca taaaccagtt tgtccggggc ctgactttct
    68641 taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata
    68701 ccacctgttt tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca
    68761 gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat
    68821 caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt
    68881 tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact
    68941 gggtgacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    69001 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat
    69061 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    69121 atggtcattg cggacaggta caactcacgt tgtagaaatg gctgggggcg aaaatgctga
    69181 caataaaacg tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca
    69241 atctcacatc gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg
    69301 ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt
    69361 cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg
    69421 acaaacgata tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc
    69481 ctattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg
    69541 ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc
    69601 gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa
    69661 aagcgggaca acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag
    69721 gtcaggcgct gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcgggac
    69781 aggtagtcag cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta
    69841 aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg
    69901 aataaaccat gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc
    69961 gcgaaaccac tgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca
    70021 gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata
    70081 tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg
    70141 acaccggcaa aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact
    70201 gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc
    70261 aaaacggtaa tggatcatga gcagcaacat ctctctatgg ggcagatcaa ctgcagccag
    70321 ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag
    70381 ttacgcaaga aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa
    70441 attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa
    70501 ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg
    70561 ctatatgggg cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc
    70621 tgaatattat ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc
    70681 aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc
    70741 cagcacgacc gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg
    70801 catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg
    70861 caacagaaac gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca
    70921 gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg
    70981 tttcaacgga ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc
    71041 aattttcact ggcgttttaa tgtcttggcg ggaataaaag ccaggagcca acactttcac
    71101 tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc
    71161 atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat
    71221 gcccaacgca accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat
    71281 caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa
    71341 aatggtgcca agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta
    71401 atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa
    71461 ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat
    71521 tgctccagca tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg
    71581 cggtagctga tacaccagct gcaggatgcc gccgaccgta acagcccacg ccagcgccag
    71641 caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag
    71701 cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac
    71761 cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta gctggctggt
    71821 cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg
    71881 tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac
    71941 ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat
    72001 cggtacaaat gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag
    72061 tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat
    72121 tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag
    72181 cgattttaat aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc
    72241 aggcgctgaa agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt
    72301 acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca
    72361 ctgcctattc gccggcggtt tgcggaactg tctgattttg cacgcattca atgaagtgcc
    72421 gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat
    72481 tcactacacc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg
    72541 caccgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag
    72601 tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt
    72661 cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg
    72721 tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc
    72781 tattgctacg atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact
    72841 caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg
    72901 ccgccagttc caccagcctt tcagcatcgc gcagattttc tgccagcggt ttatcgacac
    72961 agacatgtac ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg
    73021 agtgcacaaa aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa
    73081 tgcgccagct gtcacaaatt ggcagggctt tggcgcgcgt aggcgactag gctccttgta
    73141 acgtccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta
    73201 atcccactac gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa
    73261 tcaagacgtt gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa
    73321 tcaccgtcaa ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg
    73381 aaaagatgca tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa
    73441 ggaccatctt cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg
    73501 aattcataca ttcgcgcggc gcggctgcgc agttcaccca gcgtctgggc accacgcaat
    73561 aacaacgtgg tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa
    73621 ttacaaaaac gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga
    73681 taatgacgtt tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg
    73741 accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcagg
    73801 tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg
    73861 gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt
    73921 taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata
    73981 gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat
    74041 ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt
    74101 cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata
    74161 gcaggtatga aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt
    74221 ttcgtccggg tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat
    74281 catccctagc ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc
    74341 ccagggcttg aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg
    74401 atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact
    74461 gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta
    74521 ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt
    74581 cacatatttg ataaaaactc tctatcaaag cgacgttttg ataaaaaaat attgtccccg
    74641 aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc
    74701 gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat
    74761 cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt
    74821 cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt
    74881 tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc
    74941 gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca
    75001 cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga
    75061 tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt
    75121 ctcgctgttg atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg
    75181 gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg
    75241 tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt
    75301 tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct
    75361 ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga
    75421 cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt
    75481 aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt
    75541 gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct
    75601 acaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc
    75661 gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt
    75721 tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt
    75781 tgacctgggc aaatcgacgc accagccaga gcttccgtgg atgatgctgg gaattattgg
    75841 catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt
    75901 gctggaacgc gacgcctgat ttgatacacc cccctgactc ctccatactg aaagtgaact
    75961 gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct
    76021 caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa
    76081 cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa
    76141 agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga
    76201 cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt
    76261 caatggctac gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt
    76321 ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt
    76381 tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc
    76441 gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat
    76501 tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg
    76561 cgttgctgat taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat
    76621 ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt
    76681 tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg
    76741 cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca
    76801 gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta
    76861 accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg
    76921 acgcccatat tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg
    76981 ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga
    77041 ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc
    77101 tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc
    77161 aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt
    77221 acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca
    77281 ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc
    77341 gcgcccttta tttttcgtgc aaaggaaaac atttccgctt atcctttgtg tccggcaaaa
    77401 acatcccttc agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc
    77461 gccgtccaga cgactggcac cttcacctcc gcgatggcga catgttacaa actgtcgtgc
    77521 cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga
    77581 ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc
    77641 acgatttcac cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg
    77701 agcgcggatt taatgaaggc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca
    77761 ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg
    77821 aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt
    77881 ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg
    77941 cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg
    78001 gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata
    78061 tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc
    78121 accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg
    78181 attctgcgcc acatacacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca
    78241 acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc
    78301 actttgaagc attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca
    78361 cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg
    78421 acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat
    78481 cgtagcttcc tgttgtcatt aggttatttt acctgtataa ataaccagta tattcaacag
    78541 ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct
    78601 attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa
    78661 ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa
    78721 gaagataaac agcgcattag cgaaattccc caggaaacgt gggaaagcgc cgatgactgg
    78781 tttgtcagcg aataatatgc agtgattttt gtttttgccg ggtcgccccg gcttttttat
    78841 tttacttacg aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg
    78901 ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt
    78961 tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg
    79021 cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc
    79081 catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag
    79141 caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc
    79201 cgagcaggaa gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca
    79261 atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa
    79321 cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc
    79381 tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag
    79441 cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc
    79501 cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc
    79561 accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga
    79621 aggcgaaaag tatgtcccgt tagtcctccg cgcgcaaacg ctgtgggatg aactttcccg
    79681 ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga
    79741 ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa
    79801 gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat
    79861 cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat
    79921 ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg
    79981 tcatgacgat gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc
    80041 gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt
    80101 acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc
    80161 ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga
    80221 cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt
    80281 tccgtttgcg gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt
    80341 gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga
    80401 agattttatt atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag
    80461 tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga
    80521 ccaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt
    80581 acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat
    80641 ctcatcaata atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca
    80701 attatggatc tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa
    80761 tatttaatct ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag
    80821 cactcaccag acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta
    80881 attcaaaatt attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt
    80941 cattctccga cacgccattt tctttctctc gcaaattcgt attggatgca cttatgtcat
    81001 tcacaaatac ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa
    81061 ttattgtcta cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg
    81121 gctggtatca caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc
    81181 tggttattcg cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt
    81241 cgcccatgac tcgtcttgcc gcaaaagctg gacatctcgc attgtaccta ttgctgttcg
    81301 ccatcggcat cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg
    81361 gctggtttga cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg
    81421 ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac ggatttatgg
    81481 ccctgaagca tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt
    81541 catctgacta tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc
    81601 tgatgttctc tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc
    81661 acgcctttgt taacttccgc atacagcatc ttggctatag ctggttatat ggcaccttta
    81721 aagatttcga cggtactttt acctttgatg aaaaaaatcc ggctgccgat aaagtgaatg
    81781 tgacaattaa caccaccagc gtcgatacta accacgccga acgcgataaa catcttcgca
    81841 gtgcggattt tctcaatacc acaaaatatc cacaggcaac attcacctcc accagcgtga
    81901 agaaagacgg tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac
    81961 ccgtcacgct ggaagcgaaa ttaattggtc agggcgacga cccatggggc ggtaaacgtg
    82021 caggctttga ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag
    82081 gtccagcttc tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag
    82141 agtgaaatgt tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa
    82201 tacccagtgt tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac
    82261 gtcctgcccg acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct
    82321 cactacaaca acctttgtat ttttccgcgc atactggaca ctgaataaac agcaggtggc
    82381 agccatcatt tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg
    82441 cgataatctc atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa
    82501 taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct
    82561 cgatatgcca gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac
    82621 gaatgccgcc ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga
    82681 ctgcttttgg cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt
    82741 gccccacttc atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa
    82801 gcatggcgtt cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag
    82861 gatcgtcaat accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg
    82921 atttcccgtc atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg
    82981 catagagctg cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa
    83041 tgccctcatg cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata
    83101 aagcgtcacg ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg
    83161 ttcgcggttc gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt
    83221 gtaacactgg catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat
    83281 atagcaatta tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt
    83341 atttgttatt tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg
    83401 gcgaaaaaat gcgacaatac atacaattgc ccgtataggt tgaaaaacag gattgatatg
    83461 acgaatctac ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg
    83521 ggtattggcg tactttggtt ggtcgtgcaa ttgccctacc cgattatcta ccgcctcggt
    83581 tgtggattag gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc
    83641 aacctggaac tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag
    83701 aatttcgaat ccgttggcat gggcctgatg gaaaccggta tggcgtggtt ctggccggac
    83761 cgccgaatcg cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg
    83821 caaaaacgcg gcatcctgtt agttggcatc cattttctga cactggagct gggcgcgcgg
    83881 cagtttggta tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt
    83941 gactggctac aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat
    84001 ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accagatcat
    84061 gattacggcc cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc
    84121 acgaccggaa cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca
    84181 cgccgtaagc cagatggcaa agggtatcaa ttgattatgc tgccgccaga gtgttctccg
    84241 ccactggatg atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc
    84301 atgatggcac cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc
    84361 gttccttcac gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc
    84421 acagcaaatc cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg
    84481 gcgcataatt agtgtgttta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag
    84541 cggattgcta tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc
    84601 gtcgcctggc taggctgttt tcttaccggg gccgccttca gtctggtaat gcccttctta
    84661 cccctctacg ttgagcagct tggtgttacc ggccactccg ccctgaatat gtggtccggt
    84721 attgtcttca gtataatggg ttcctaaaca catcatttta gatattgaat atgagacggg
    84781 caatcagcta catcagattt tcaagtgaac gacaactgaa aggggactct gtacgccgac
    84841 aatctaagct ggttactgac tggcttgata agaaccctga gttttactgg gattcatcct
    84901 tgagctttaa agatttgggg aaaagtgctt ttagtggtaa gcacctgaaa ggtggtctag
    84961 gtgacttttt aaccgctatc gagaaagggc ttgttaaagc tggtgatacg ctactaatcg
    85021 agagtttaga ccgtctgtca cgtcaggata ttgatattgc ttctgagcta ttacgccgta
    85081 tcttaagggc tggtgtagat gtagtcacac tgagcgatgg agaacattac acacgagaaa
    85141 gtttaaaaga cccgttagcc ctgattaaaa gcattctgat aatgcagcgg gcgcatgagg
    85201 aaagtttgag gaagtctgag cgtgtccaag ctgcatggaa ccgtaaaaaa gaactcatct
    85261 ccgaaggtat caaggtatct aggcgttgtc ctgcatggtt aaggctgaat gatgaccgtc
    85321 gaacattcac aatcattcct gataaagtgg aagttgttaa acgagcattt gacctgagat
    85381 tacagggttt aagcttttgg gctatcacca gaactttaaa cgatgaaggt cacttgtcat
    85441 tgaatcaata cacacccaaa caaaaggggt ggtctgatac tgctgtaaag aaactactca
    85501 gaaatcgtgc tgtgattgga tgtttcacac ctgctggacg tgaagaagtt cagggttact
    85561 accccgcaat aatcagtgag tcactattct atcgagtaca gcaactgaac acagggcaat
    85621 atggtagggc ttctgtatca agtaatccgc tatcagtcaa cttgttcagg ggtatcatca
    85681 aatgttctga gtgtggggct actattgttc ttggtggata tgccttaaaa cgttttggaa
    85741 tgtacagatg ccctaatcgt tcagctaaca ggtgtagtgc aaaggctatc agtcgtaagc
    85801 aaacggatgc aactatcctt tacatgctcg cattatgcga ccgttttgaa actgaaaata
    85861 ctgacactat tgatagcctt aaattacagc gtgaagattt gc
//
LOCUS       DADLZM010000003        82566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLZM010000003 DADLZM010000000
VERSION     DADLZM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.003"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..368)
                     /locus_tag="JNY73_000188"
     CDS             complement(<1..368)
                     /locus_tag="JNY73_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8046325.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(439..2553)
                     /gene="fusA"
                     /locus_tag="JNY73_000189"
     CDS             complement(439..2553)
                     /gene="fusA"
                     /locus_tag="JNY73_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY8046326.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="JNY73_000190"
     CDS             complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="JNY73_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY8046327.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="JNY73_000191"
     CDS             complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="JNY73_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY8046328.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="JNY73_000192"
     CDS             complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="JNY73_000192"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY8046329.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4012..4371)
                     /gene="tusC"
                     /locus_tag="JNY73_000193"
     CDS             complement(4012..4371)
                     /gene="tusC"
                     /locus_tag="JNY73_000193"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY8046330.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4371..4757)
                     /gene="tusD"
                     /locus_tag="JNY73_000194"
     CDS             complement(4371..4757)
                     /gene="tusD"
                     /locus_tag="JNY73_000194"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY8046331.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4757..5479)
                     /locus_tag="JNY73_000195"
     CDS             complement(4757..5479)
                     /locus_tag="JNY73_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8046332.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5646..6458)
                     /gene="fkpA"
                     /locus_tag="JNY73_000196"
     CDS             complement(5646..6458)
                     /gene="fkpA"
                     /locus_tag="JNY73_000196"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8046333.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6679..6897
                     /locus_tag="JNY73_000197"
     CDS             6679..6897
                     /locus_tag="JNY73_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY8046334.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6946..7536)
                     /gene="slyD"
                     /locus_tag="JNY73_000198"
     CDS             complement(6946..7536)
                     /gene="slyD"
                     /locus_tag="JNY73_000198"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8046335.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7631..7831)
                     /locus_tag="JNY73_000199"
     CDS             complement(7631..7831)
                     /locus_tag="JNY73_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY8046336.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7841..9646)
                     /gene="kefB"
                     /locus_tag="JNY73_000200"
     CDS             complement(7841..9646)
                     /gene="kefB"
                     /locus_tag="JNY73_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY8046337.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9646..10197)
                     /gene="kefG"
                     /locus_tag="JNY73_000201"
     CDS             complement(9646..10197)
                     /gene="kefG"
                     /locus_tag="JNY73_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY8046338.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10328..12241
                     /locus_tag="JNY73_000202"
     CDS             10328..12241
                     /locus_tag="JNY73_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8046339.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRQEAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12241..13263
                     /locus_tag="JNY73_000203"
     CDS             12241..13263
                     /locus_tag="JNY73_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8046340.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYLLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13257..13475
                     /locus_tag="JNY73_000204"
     CDS             13257..13475
                     /locus_tag="JNY73_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY8046341.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13529..14398
                     /locus_tag="JNY73_000205"
     CDS             13529..14398
                     /locus_tag="JNY73_000205"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY8046342.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14453..14857)
                     /locus_tag="JNY73_000206"
     CDS             complement(14453..14857)
                     /locus_tag="JNY73_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY8046343.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15159..15791
                     /gene="crp"
                     /locus_tag="JNY73_000207"
     CDS             15159..15791
                     /gene="crp"
                     /locus_tag="JNY73_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY8046344.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15842..17932
                     /locus_tag="JNY73_000208"
     CDS             15842..17932
                     /locus_tag="JNY73_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046345.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17999..19219)
                     /gene="argD/dapC"
                     /locus_tag="JNY73_000209"
     CDS             complement(17999..19219)
                     /gene="argD/dapC"
                     /locus_tag="JNY73_000209"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY8046346.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19305..19868)
                     /gene="pabA"
                     /locus_tag="JNY73_000210"
     CDS             complement(19305..19868)
                     /gene="pabA"
                     /locus_tag="JNY73_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY8046347.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19900..20502)
                     /locus_tag="JNY73_000211"
     CDS             complement(19900..20502)
                     /locus_tag="JNY73_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY8046348.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20492..20659)
                     /locus_tag="JNY73_000212"
     CDS             complement(20492..20659)
                     /locus_tag="JNY73_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY8046349.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20764..21336)
                     /gene="ppiA"
                     /locus_tag="JNY73_000213"
                     /gene_synonym="rotA"
     CDS             complement(20764..21336)
                     /gene="ppiA"
                     /locus_tag="JNY73_000213"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY8046350.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21607..22788
                     /gene="tsgA"
                     /locus_tag="JNY73_000214"
     CDS             21607..22788
                     /gene="tsgA"
                     /locus_tag="JNY73_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY8046351.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23050..25593
                     /locus_tag="JNY73_000215"
     CDS             23050..25593
                     /locus_tag="JNY73_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY8046352.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHSSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25590..25916
                     /gene="nirD"
                     /locus_tag="JNY73_000216"
     CDS             25590..25916
                     /gene="nirD"
                     /locus_tag="JNY73_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY8046353.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26042..26848
                     /gene="nirC"
                     /locus_tag="JNY73_000217"
     CDS             26042..26848
                     /gene="nirC"
                     /locus_tag="JNY73_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY8046354.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWIALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26867..28240
                     /gene="cobA"
                     /locus_tag="JNY73_000218"
     CDS             26867..28240
                     /gene="cobA"
                     /locus_tag="JNY73_000218"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY8046355.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28487..28654
                     /locus_tag="JNY73_000219"
     CDS             28487..28654
                     /locus_tag="JNY73_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY8046356.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28949..30286
                     /gene="frlA"
                     /locus_tag="JNY73_000220"
                     /pseudo
     CDS             28949..30286
                     /gene="frlA"
                     /locus_tag="JNY73_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30307..31329
                     /gene="frlB"
                     /locus_tag="JNY73_000221"
     CDS             30307..31329
                     /gene="frlB"
                     /locus_tag="JNY73_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY8046357.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31379..32208
                     /gene="frlC"
                     /locus_tag="JNY73_000222"
                     /pseudo
     CDS             31379..32208
                     /gene="frlC"
                     /locus_tag="JNY73_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32205..32990
                     /gene="frlD"
                     /locus_tag="JNY73_000223"
     CDS             32205..32990
                     /gene="frlD"
                     /locus_tag="JNY73_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY8046358.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33090..33821
                     /gene="phoB"
                     /locus_tag="JNY73_000224"
     CDS             33090..33821
                     /gene="phoB"
                     /locus_tag="JNY73_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY8046359.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33981..35066)
                     /locus_tag="JNY73_000225"
     CDS             complement(33981..35066)
                     /locus_tag="JNY73_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY8046360.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35078..36382)
                     /locus_tag="JNY73_000226"
     CDS             complement(35078..36382)
                     /locus_tag="JNY73_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046361.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36394..36747)
                     /locus_tag="JNY73_000227"
     CDS             complement(36394..36747)
                     /locus_tag="JNY73_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY8046362.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36758..37636)
                     /locus_tag="JNY73_000228"
     CDS             complement(36758..37636)
                     /locus_tag="JNY73_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY8046363.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELV
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37633..38859)
                     /locus_tag="JNY73_000229"
     CDS             complement(37633..38859)
                     /locus_tag="JNY73_000229"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY8046364.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38860..40023)
                     /locus_tag="JNY73_000230"
     CDS             complement(38860..40023)
                     /locus_tag="JNY73_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY8046365.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40107..40469)
                     /locus_tag="JNY73_000231"
     CDS             complement(40107..40469)
                     /locus_tag="JNY73_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY8046366.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40486..41391)
                     /locus_tag="JNY73_000232"
     CDS             complement(40486..41391)
                     /locus_tag="JNY73_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046367.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41661..42665)
                     /gene="trpS"
                     /locus_tag="JNY73_000233"
     CDS             complement(41661..42665)
                     /gene="trpS"
                     /locus_tag="JNY73_000233"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY8046368.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42658..43416)
                     /gene="gph"
                     /locus_tag="JNY73_000234"
     CDS             complement(42658..43416)
                     /gene="gph"
                     /locus_tag="JNY73_000234"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY8046369.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43409..44086)
                     /gene="rpe"
                     /locus_tag="JNY73_000235"
     CDS             complement(43409..44086)
                     /gene="rpe"
                     /locus_tag="JNY73_000235"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY8046370.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44104..44940)
                     /gene="dam"
                     /locus_tag="JNY73_000236"
     CDS             complement(44104..44940)
                     /gene="dam"
                     /locus_tag="JNY73_000236"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY8046371.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45047..46333)
                     /gene="damX"
                     /locus_tag="JNY73_000237"
     CDS             complement(45047..46333)
                     /gene="damX"
                     /locus_tag="JNY73_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY8046372.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46425..47513)
                     /gene="aroB"
                     /locus_tag="JNY73_000238"
     CDS             complement(46425..47513)
                     /gene="aroB"
                     /locus_tag="JNY73_000238"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY8046373.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47570..48091)
                     /gene="aroK"
                     /locus_tag="JNY73_000239"
     CDS             complement(47570..48091)
                     /gene="aroK"
                     /locus_tag="JNY73_000239"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY8046374.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48492..49730)
                     /gene="hofQ"
                     /locus_tag="JNY73_000240"
     CDS             complement(48492..49730)
                     /gene="hofQ"
                     /locus_tag="JNY73_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY8046375.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQASSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49642..50046)
                     /gene="hofP"
                     /locus_tag="JNY73_000241"
     CDS             complement(49642..50046)
                     /gene="hofP"
                     /locus_tag="JNY73_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY8046376.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50036..50476)
                     /gene="hofO"
                     /locus_tag="JNY73_000242"
     CDS             complement(50036..50476)
                     /gene="hofO"
                     /locus_tag="JNY73_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY8046377.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50460..50999)
                     /locus_tag="JNY73_000243"
     CDS             complement(50460..50999)
                     /locus_tag="JNY73_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY8046378.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50999..51778)
                     /gene="hofM"
                     /locus_tag="JNY73_000244"
     CDS             complement(50999..51778)
                     /gene="hofM"
                     /locus_tag="JNY73_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY8046379.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51898..54450
                     /gene="mrcA"
                     /locus_tag="JNY73_000245"
     CDS             51898..54450
                     /gene="mrcA"
                     /locus_tag="JNY73_000245"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY8046380.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54615..55175)
                     /gene="nudE"
                     /locus_tag="JNY73_000246"
     CDS             complement(54615..55175)
                     /gene="nudE"
                     /locus_tag="JNY73_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY8046381.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55495..57630
                     /gene="igaA"
                     /locus_tag="JNY73_000247"
     CDS             55495..57630
                     /gene="igaA"
                     /locus_tag="JNY73_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY8046382.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRQRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLVLNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57695..58363
                     /gene="yrfG"
                     /locus_tag="JNY73_000248"
     CDS             57695..58363
                     /gene="yrfG"
                     /locus_tag="JNY73_000248"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY8046383.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58374..58775
                     /gene="hslR"
                     /locus_tag="JNY73_000249"
     CDS             58374..58775
                     /gene="hslR"
                     /locus_tag="JNY73_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY8046384.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58800..59678
                     /gene="hslO"
                     /locus_tag="JNY73_000250"
     CDS             58800..59678
                     /gene="hslO"
                     /locus_tag="JNY73_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY8046385.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59813..61537)
                     /locus_tag="JNY73_000251"
     CDS             complement(59813..61537)
                     /locus_tag="JNY73_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY8046386.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61916..63538
                     /gene="pckA"
                     /locus_tag="JNY73_000252"
     CDS             61916..63538
                     /gene="pckA"
                     /locus_tag="JNY73_000252"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY8046387.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63614..64966)
                     /gene="envZ"
                     /locus_tag="JNY73_000253"
     CDS             complement(63614..64966)
                     /gene="envZ"
                     /locus_tag="JNY73_000253"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY8046388.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64963..65682)
                     /gene="ompR"
                     /locus_tag="JNY73_000254"
     CDS             complement(64963..65682)
                     /gene="ompR"
                     /locus_tag="JNY73_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY8046389.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65874..66386
                     /gene="greB"
                     /locus_tag="JNY73_000255"
     CDS             65874..66386
                     /gene="greB"
                     /locus_tag="JNY73_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY8046390.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            66483..68804
                     /locus_tag="JNY73_000256"
     CDS             66483..68804
                     /locus_tag="JNY73_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY8046391.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQYPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69242..69469
                     /gene="feoA"
                     /locus_tag="JNY73_000257"
     CDS             69242..69469
                     /gene="feoA"
                     /locus_tag="JNY73_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY8046392.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69486..71807
                     /gene="feoB"
                     /locus_tag="JNY73_000258"
     CDS             69486..71807
                     /gene="feoB"
                     /locus_tag="JNY73_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY8046393.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71807..72043
                     /gene="feoC"
                     /locus_tag="JNY73_000259"
     CDS             71807..72043
                     /gene="feoC"
                     /locus_tag="JNY73_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY8046394.1"
                     /translation="MASLIQVRDLLALRCRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72246..73124
                     /gene="rpnA"
                     /locus_tag="JNY73_000260"
     CDS             72246..73124
                     /gene="rpnA"
                     /locus_tag="JNY73_000260"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY8046395.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73197..73466
                     /locus_tag="JNY73_000261"
     CDS             73197..73466
                     /locus_tag="JNY73_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY8046396.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73460..73825
                     /locus_tag="JNY73_000262"
     CDS             73460..73825
                     /locus_tag="JNY73_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY8046397.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73826..74614)
                     /gene="bioH"
                     /locus_tag="JNY73_000263"
     CDS             complement(73826..74614)
                     /gene="bioH"
                     /locus_tag="JNY73_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY8046398.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74652..75335
                     /gene="gntX"
                     /locus_tag="JNY73_000264"
     CDS             74652..75335
                     /gene="gntX"
                     /locus_tag="JNY73_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY8046399.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75394..75969
                     /gene="nfuA"
                     /locus_tag="JNY73_000265"
     CDS             75394..75969
                     /gene="nfuA"
                     /locus_tag="JNY73_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY8046400.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76330..77634
                     /gene="gntT"
                     /locus_tag="JNY73_000266"
     CDS             76330..77634
                     /gene="gntT"
                     /locus_tag="JNY73_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY8046401.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77679..79763)
                     /gene="malQ"
                     /locus_tag="JNY73_000267"
     CDS             complement(77679..79763)
                     /gene="malQ"
                     /locus_tag="JNY73_000267"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY8046402.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79773..82166)
                     /gene="malP"
                     /locus_tag="JNY73_000268"
     CDS             complement(79773..82166)
                     /gene="malP"
                     /locus_tag="JNY73_000268"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY8046403.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19791 a        22425 c        20840 g        19510 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcat attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcacgagca
      241 gcaccgccgt aggttttagc cagtacggtg gtgattgcag cggtcagagt agttttaccg
      301 tggtcaacgt ggccgatagt accaacgtta acgtgcggtt ttgtacgttc aaatttttct
      361 ttagacacgg ctatattcct tactatagtg ctctcccctt caggagagag cacgggactt
      421 tggtattaac ccttaggctt atttaccacg ggcttcaatt acggcctgag caacgttact
      481 cggcgcttca tcatacttca ggaattccat agtgtatgat gcacgacctt tggtcagaga
      541 acgcagctga gttgcgtatc cgaacatttc agacagcggt acttcagcgt ggatcttaac
      601 gccagtaact tcagattcct gacctttgag cataccacga cgacggctca agtcaccgat
      661 aacgtcaccg gtgttctctt ccggagtttc tacttcaacc ttcatgatcg gctcaagcag
      721 aactggtttc gctttcttaa agccttcttt aaaggcgata gaagcagcca gtttaaacgc
      781 cagttcagag gagtcaacgt catggtaaga accgaagtgc agacgaatac ccatgtctac
      841 taccgggtag cctgccagcg gacctgcttt cagctgttcc tggatacctt tatcaacggc
      901 cgggatgtat tcgccaggga ttacaccacc tttaatgtcg ttgatgaact cgtagccttt
      961 cgggtttgaa cccggctcca gcgggtacat gtcgataaca acatgaccat actgaccacg
     1021 accaccagac tgtttcgcgt gtttaccttc aacatcggta actttctggc ggatagtttc
     1081 acggtaagca acctgcggtt tacctacgtt cgcttcaacg ttgaattcac gcttcatacg
     1141 gtcaacgatg atgtcgaggt gcagttcgcc catacccgcg atgatggtct ggttagattc
     1201 ttcgtcagtc catacacgga aagacgggtc ttctttagcc agacggccca gagccagacc
     1261 cattttttcc tggtcagctt tggttttcgg ttcaactgcg atggagatta ccggctcagg
     1321 gaattccata cgttccagaa tgatcggcgc atccgggtca cacagggtgt caccagtggt
     1381 tacgtctttc agaccgatag cagcagcgat gtcgcccgcg cgaacttctt tgatctcttc
     1441 acgtttgtta gcgtgcatct gaacgatacg accgaaacgc tcacgtgcag ctttcacgga
     1501 gttcagtacg gtatcaccag agttaaccac accggagtaa acacggaaga aggtcaggtt
     1561 accaacaaac gggtcggtag cgattttgaa cgccagtgca gagaacggct cgtcatcact
     1621 tgcgtgacgt tcagccggag tgtctttacc gtcgtccagg ataccgttga tcgcaggtac
     1681 gtcaaccggg gatggcaggt aatcaattac cgcatccagc atcgcctgaa cacctttgtt
     1741 cttgaacgca gaaccacagg ttaccaggat gatttcgttg ttcagaacgc gctgacgcag
     1801 agcacctttg atttctgctt cagtcagttc ttcaccaccc aggtattttt ccatcagctc
     1861 ttcagaagct tcagctgcgg attcgatcag gttctggtgc cattcgttag ccagttcaac
     1921 catgtctgcc gggatatctt cgtattcgaa ggttacgccc tggtcagcgt cgttccagtt
     1981 gatagctttc attttcacca ggtcaacaac accggtgaaa tgttcttcag caccaatcgc
     2041 cagctgcagc ggaaccgggt tcgcgcccag acgggttttg atctggttaa caactttcag
     2101 gaagttcgca cccatgcggt ccattttgtt aacgaacgca atgcgcggaa ctttatattt
     2161 gtttgcctga cgccatacgg tttcagactg cggctgaaca ccaccaactg cgcagtaaac
     2221 cattaccgca ccatcgagaa cacgcatgga acgttctact tcgattgtga agtcaacgtg
     2281 ccccggggtg tcgatgatgt tgatgcgatg cggctcatac tgcttagcca taccagacca
     2341 gaatgcagta gtcgcagcgg aagtgatggt aataccacgt tcctgctcct gctccatcca
     2401 gtccatggtt gcagcgccgt catgaacttc accgatttta tggtttacac cggtgtagaa
     2461 cagaatacgt tcggtagtag tggttttacc ggcgtcgatg tgcgcactga taccgatgtt
     2521 acggtagcgt gcgatgggtg ttgtacgagc catttgtttc ctcgtttatc ttttaggcgt
     2581 tcaatttaag tagcccaaag cgggctgctt actggaagcg cccgcctggt gactaaaact
     2641 ccgaagggat taccaacggt agtgtgcgaa cgccttgttg gcttcggcca tacggtgaac
     2701 gtcttcacgt ttcttaactg cagtaccttt gttttctgca gcatcagaaa gttcgttcgc
     2761 caggcgcaga gccatggatt tatcaccgcg tttacgagca gcttcaacga tccaacgcat
     2821 tgccagagca ttacgacgaa ccggacggac ttcaactggt acctgataag tagaaccacc
     2881 aacgcggcga gacttaactt ctacagtcgg gcgcacgttt tcgagagcta cttcgaatgc
     2941 ttccagttca gatttaccag agcgctgagc cagggtctcc agcgcgctgt atacgataga
     3001 ttcagcagta gattttttac catctaccat caggatattt acaaatttag ccagcagttc
     3061 tgatccgaac ttcggatccg gcagaatttt acgctgacca atgacgcgac gacgtggcat
     3121 ggaaatactc cgttgttaat tcaggattgt ccaaaactct acgagtttag tttgacattt
     3181 aagttaaaac gtttggcctt acttaacgga gaaccattaa gccttagggc gcttcacgcc
     3241 atacttggaa cgagcctgct tacggtcttt aacgccggag cagtcaagcg caccacgtac
     3301 ggtgtggtaa cgaacacccg ggaggtcttt aacacgaccg ccacggatca ggatcacgga
     3361 gtgctcctgc aggttgtgac cttcaccacc gatgtaggaa gtcacttcga aaccgttagt
     3421 cagacgaaca cggcatactt tacgcagcgc ggagttcggt tttttaggag tggtagtata
     3481 tacacgagta catacgccac gtttttgcgg gcatgcttcc agcgcaggca cgttgctttt
     3541 cgcaactttg cgagcacgtg gtttgcgtac cagctggtta actgttgcca ttaaatagct
     3601 cctggtttta gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac
     3661 gccgaatttt agggcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcac
     3721 caggccatct ggctggagtg cttaaccgta agtctgacga aatcagtata gtcaatggga
     3781 atgatgtcgt tcaaaatttg accagtcaaa ccgcgggcaa taaggtcttc gttcagggca
     3841 tagaccttaa tgggggcatt acgcagactt tcaaggtagc ggttaccgtc aactgcggca
     3901 gttacgccat cttgcaataa tagcagttcg tctccttcac tgagcagacg cagcagcgca
     3961 gcaaaatccg tcagccaggg tgaacgatgt aacgtgtgca gcataaacgc ctcaaaacct
     4021 caaaataaca tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc
     4081 ttcgacaaca aagggtgtct gcggatctaa cccgcgttcg cgcagtgaag ccgcacaaac
     4141 ccagcactgt tcaatgtcgt acagactcaa caatttaaaa gtggcaatgt aatcacgcgc
     4201 cagcactgca tcgggctttt gtcctgacag cagctgaaaa acgccatcag caataaagaa
     4261 gacggccaga tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccgacc
     4321 agcagctgta ccatgaggtg cagtagaaaa aacaaacgca attcgtttca tcagaactgt
     4381 accaccctgt cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat
     4441 cccggctgaa ggtttgacga agccagcccc agccttcctg cttcagtttc atcgacgata
     4501 ccgcggcgta acgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac
     4561 tgttgccagc cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcag ttggttagcg
     4621 ttatagaccc cttcccgata gaaaaagacg ctgcttaact tatggccttc tgctatcagc
     4681 gcctgcgcaa actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc
     4741 acgatggcaa aacgcattac ttatcttgcc cctgaaaatc accgctcttg aactggcgga
     4801 tgtagagata gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcat
     4861 ctttaatatc gaagatccct ttctcgtaga gattcagcac gatctgacga tttttggcgt
     4921 tattagaaac attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa
     4981 ccagatcttc aacagaagag gcaaagttga cgcttgaacc gacatccggg gtttctggcg
     5041 gcacaaaagt gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca
     5101 gaccaattac acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc
     5161 cgcttttggc gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt
     5221 gcagcatacg tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac
     5281 cgttagcaat gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac
     5341 agtgggagcc aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat
     5401 caaaatcggt ctggtcgaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg
     5461 ttaaaagcga cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt
     5521 cagggcaggc tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc
     5581 agcactaatt atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt
     5641 aatgcttatt ttttagcaga atctgcggct ttcgcatcag cttccggctt tgcatcagcc
     5701 ttcggcgctg gtttcacatc cagcagctct acgtcaaaca ctagggtaga attcggtggg
     5761 atccccggaa cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta
     5821 ccgcctttct tgatgttctt cagaccttct gtccaacccg ggataacacc gtccagacgg
     5881 aaagaaagcg gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta
     5941 cctttgtagt tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct
     6001 actacctgat aaaccagacc agttgaagag gttttcacac ctttctcttt ggcaaatttc
     6061 tcgcggtact ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga
     6121 gcagaagact tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggag
     6181 agtttgctct tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt
     6241 ttgatgccca gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca
     6301 cccagtgcat aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt
     6361 gtagcaggtt ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt
     6421 gtggtcgcca gtagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca
     6481 tctcacccca gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta
     6541 catacagaca cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga
     6601 tgaccgcgaa atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt
     6661 cgacaaaaga ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag
     6721 agccgactgg cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat
     6781 gaaatggaga tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc
     6841 agccagccgt cgaacatcgc gtcgcaggct gaagaaacgc caccgccaca ttattgaggc
     6901 gtaaaaaaag cggggattcc ccgctttttt gtcacctttt cggtattagt ggcaaccaca
     6961 accgccgttg cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg
     7021 gccatgaccg ccgcagcaac cgtcgtggtc gtgatcgtgg tgatgatcgt gcgcgccgtg
     7081 aacgtggcca tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt
     7141 gaatttcagg ttctgaccgg ccagcatgtg gttaccatca accactacgt gatcgtcttc
     7201 aaccgcagtg atttcaaccg gtaccggacc ctggtcagtt tcagccagga aacgcatacc
     7261 tacctgcagt tcatcaacgc ccataaatac gtctttagga acacgttgca ctaggttttc
     7321 gtcgtactga ccgtaagcgt cgttcgcgcc aacagcgaca tcaaatttgt cgccaacttc
     7381 atgaccttcc agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc
     7441 cagcggcgca ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata
     7501 ggccaggctg accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa
     7561 tagtggcgca gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg
     7621 gcgcatatcg ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg tgaacgtgat
     7681 cgcgggcttc tttgtctgct tctcgcatct gatgtccgca cttaacacat tcaacaatat
     7741 caatattatt ttcgcgccac atcgccattg aatcctgcgc ctgacaggcc gggcattttg
     7801 cgcccgctat aaaacgtttt cggattgcca tccttaccct ctactcaaat tcatcccagc
     7861 cgtccagctg gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc
     7921 gggcttccct ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca
     7981 gcattcgcat atccaggcgg cgaaaatgca gttgcgcccg ctgtgcctga tgcggatgca
     8041 tgccaagcgt gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg
     8101 aaaactgcgt cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcactcgcg
     8161 caagaatatg caaatgcgga aagtgctgct ggcatatttc caccagcttc atggtgtctt
     8221 ccggctcgtt acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa
     8281 gatcgacctg cgtggcgtcg ccgtaataaa ctttgtagcc atatttacgc atcaggttaa
     8341 cggcgctgat atcccgctcc agcacggtaa tgcgcatttt atttgccatc agcaaacgac
     8401 caatcacctg accaaaacgc ccgaagccca cgacaatgac ctggggttta tcatcgttga
     8461 cccacggttt ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcca
     8521 ccagcttcat cagcaacggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg
     8581 ccatctggtc gccctggaat aaacgttgtg aagaagcggt agaaaagagg acaaaggcaa
     8641 actcaccgcc ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat
     8701 acaatcgcgc cagcagatac agcacgagaa ttttcaccgc caccagcaca accacgctta
     8761 tcactaccca caacagatgg gtataaagca ccccgaggtt gagcgacatg ccgacagaga
     8821 taaagaacaa accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc
     8881 gatattcact ttccgccagc agcacgcccg caataaatgt accgagcgcc atcgacagcc
     8941 ccagcgcatc cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca
     9001 cttcccgcac gccagaagct gcaataaagc ggaataccgg acgcagtaaa tagcgcccac
     9061 caatcagcat gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt
     9121 cgtctgccga ccccgccaac aacggcacta acgccagtgc tgggatcact gccagatcct
     9181 gaaacagcag aaccgaaaac ccgagctggc cggattcgct gcgattcatc cctttctcac
     9241 gcatcaattg caacgccatt gcagttgaag acatcgcaag gccaatgcca ccgaccaccg
     9301 ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca
     9361 gcacctgtgc cacgcctacg ccaaaaatcg aacgccgcag ttgccaaagt ttggagggat
     9421 tcaactcaag gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga
     9481 tctcatcgac gtcgctaata aaccccagcc cccacgggcc aattgcaatc cctgccagca
     9541 aatatcccaa cacagcgcca atccccagcc gcgatgccaa cggcaccgca gccaccgccg
     9601 cgaagagaaa cagcactcct gcgagtaaaa aatcggaacc ttccatcagc ggcctcctgg
     9661 agacagcgga tttgccaacc agtcaccgta ggctctggcg tggctcgcca gctcctttgc
     9721 gctttgccgt ctcgcccagt aaataatgat ggaacttaac caatgcatcc ggcacatgcc
     9781 cgccgccagt tcaaaggggc gcagcacatc gctcatcggg tagcgattca gcgcgtcata
     9841 acggtaagca ctttccggct cgccggtggt aatcacgtta cgccagtact ttcccgccag
     9901 ttggtttcct cccggcccgc tggcaaaacc acgacttaat acccggtcca gccactcttt
     9961 cagtagcgcc gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc
    10021 gcgcagtaat gcctgctcac gggggatatc aataaaaaaa tcgggatagt gcgcgtaaag
    10081 gtcgtgcacg gtaacattgc tgagctgcgt ggccggttta agcagtaccc ggtttgccac
    10141 cgagtcctga gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat
    10201 ccccctcccg gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg
    10261 cggcagctcg cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg
    10321 gcgccttatg attgttttct cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga
    10381 taatgccacc gccaccatca accccgggca gaaagtcggc ctggtgggta aaaacggctg
    10441 tggtaaatct accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta
    10501 cacctttccg ggaagctggc aactggcgtg ggtgaatcag gaaacgccag cgttaccgca
    10561 agcggcgctg gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct
    10621 gcacgacgcc aacgaacgta acgacgggca cgccattgcg accattcata gcaagctgga
    10681 tgctattgac gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt
    10741 cagcaatgaa caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct
    10801 taaccttgcc caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa
    10861 ccacctcgat ctcgatgccg ttatctggct ggaaaaatgg ttgaagagct atcagggcac
    10921 gctgatcctg atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca
    10981 gatcgaacaa caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg
    11041 cgccacccgt ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtggcaca
    11101 tctgcaaagt tatatcgatc gtttccgtgc caaagccacc aaagcgaagc aggcccagag
    11161 ccgcattaag atgctggaac gtatggagct gattgccccc gcacacgtcg acaacccgtt
    11221 ccgctttagc ttccgcgcgc cggaaagcct gccaaatccg ttactgaaga tggaaaaggt
    11281 cagcgcaggc tatggcgatc gcattattct cgactcgatt aaattgaatc tggtccccgg
    11341 ctcgcgcatt ggtctgttag gccgcaacgg cgcaggtaaa tcgacattaa tcaaactgtt
    11401 agccggtgaa cttgcgccag tcagcggtga aattggtctg gcgaaaggga tcaagctcgg
    11461 ctacttcgcc cagcatcaac ttgaatacct gcgcgccgac gaatcaccta ttcaacatct
    11521 ggcacgttta gcgccgcagg agctggaaca aaaactgcgt gactacctcg gcggctttgg
    11581 tttcctgggc gataaagtaa ccgaagaaac gcgccgcttc tccggtgggg aaaaggcccg
    11641 cctggtgctg gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac
    11701 taaccacctt gacctcgaca tgcgtcaggc agtcaccgaa gcattaatcg agtttgaagg
    11761 cgcgctggtt gtcgtttcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta
    11821 cctggttcac gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg
    11881 gttgagcgac gtacaaaagc aggaaaacca gaccgacgaa gcgccaaaag agaacgcgaa
    11941 cagcgcccag gcacgtaaag atcagaagcg tcaggaagcg gagctgcgtg cgcaaaccca
    12001 gccgctgcgt aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact
    12061 ggcgcaggcg gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga
    12121 gttgaccgcc tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat
    12181 ggcgtggctg gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg
    12241 atggcgcaga taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct
    12301 atgcgcggct ttagcaattg tcatctacaa actatgctgc cgcgtttgtt tcgtcgtcag
    12361 gtgaaattca ccccgtactg gcagcggctg gagttgcccg acggcgattt tgtcgatctc
    12421 gcgtggagtg aagaccccgc acaggcgaaa cataaaccgc gtttagtggt gtttcacggg
    12481 ctggaaggca gtctcaatag cccttacgcc cacggtctgg ttgaggcggc gcaaaagcgc
    12541 ggctggctgg gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac
    12601 cgcatttacc attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc
    12661 gaatttggtc atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc
    12721 tgtttgctgg caaaggaagg caatgatctc ccggttgatg cggcggtgat tgtctctgcg
    12781 ccgtttatgc tggaagcctg tagctatcat atggaaaagg gcttttcccg cgtttatcag
    12841 cgttacttgc tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga
    12901 acgctgccga ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat
    12961 ctaatcaccg ccagaattca cggctacgcc gacgctatcg actattatcg tcagtgtagc
    13021 gccatgccga tgctgaaccg gatcgccaaa ccgacgctga ttattcacgc caaagacgat
    13081 ccgtttatgg atcatcaggt gatcccaaaa ccggaaagtc tcccctcgca ggtggagtat
    13141 ctactgactg aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa
    13201 atgtggctgg agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt
    13261 tgattccgtg gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt
    13321 tacgtgaagg caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg
    13381 tcaaacgcca gctacagtgc ggagaagcgg tgctggtatg gtcggaactg cacgaaacgg
    13441 tcaatattat gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata
    13501 atagtgatag ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac
    13561 aggatccagc ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca
    13621 gttaaatctg catgcagctg aggtggaagg cgacagtttt caccgttaca cccgcccgga
    13681 aatggacatg gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc
    13741 cgaagcgaac gacttcggcc tgctggaaca aaccttcatt gaatacggtc agagcggcaa
    13801 agggaaatct cgcaaatatc tgcataccta cgacgaagcc gtaccctgga atcaggtacc
    13861 gggaacattc accccctggc aacctttacc agaacccact gatgtactgt tttatgaagg
    13921 tttacacggc ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt
    13981 cggcgtagtg cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga
    14041 gcgcgggcac tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat
    14101 caactacatc acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt
    14161 cgacacttca aacccgttcg cggcaaaagg tatcccgtcg ctggatgaaa gctttgtggt
    14221 gatccatttt cgtaatctgg aagggatcga tttcccttgg ctgctggcga tgttgcaagg
    14281 ctcattcatt tcccacatca atacgttagt ggtacctggc ggcaaaatgg ggctggcaat
    14341 ggaattaatt atgctgctgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaact
    14401 ttgctattat gccggataaa atactatccg gcttcacaac gggatagtta agtcacgcgg
    14461 caaccacttc atacgagtga gtaatattca ccgctttttc cagcatcaac gccactgaac
    14521 aatatttctc ggcagagaga tcaaccgcac gcgcaaccgc tgcgtctttc aggtcgcgac
    14581 cggtgacgat aaaatgcaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgtt
    14641 cagaggtcaa ttttacttca caatcgacca catcctgacg ccctttttgc aggatcgaaa
    14701 ccacatcgat ggcactgcaa ccacccgccg ccatcaacac catttccatc ggacttggtg
    14761 ctttatcgcc tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa
    14821 aagttaaccc ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat
    14881 tttcctgaca gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc taggtcatgc
    14941 tgaagcgaga caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca
    15001 gtaatacatt gatgtactgc atgtatgcaa aggacgtcac attaccgtgc agtacagttg
    15061 atagcccctt cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc
    15121 tggctctgga gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa
    15181 cagacccgac tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga
    15241 gcacgcttat tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg
    15301 tggcagtgct gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg
    15361 gtgattttat tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac
    15421 gtgcgaaaac cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc
    15481 aggtaaaccc ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca
    15541 cttcagagaa agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc
    15601 tgctgaacct ggcaaaacaa ccagatgcta tgactcaccc ggacggtatg caaatcaaaa
    15661 ttacccgtca ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc
    15721 tgaagatgct ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg
    15781 gcactcgtta atcccgtcgg agtggcgcgt tacctggtag cgcgccattt tgtttccccc
    15841 gatgtggcgc agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt
    15901 gctatgtttg cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gtctgctctt
    15961 ctccctcgtt cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt
    16021 caaacactta atcattggtg cgtcgctgtt tgccacctgt agcttactga cacagctgct
    16081 actggtaaaa gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt
    16141 cactgctgag ctggggccat tgcacgcaaa attgcttcct gcatcgctgc tcgccgccat
    16201 ttttaccctc agtctggcgg gatacatgcc ggtctgggaa ccattgctca tctatgcgtt
    16261 gggcactctc tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc
    16321 gctgcgcgag tcactaagtc tgctgtaccg tgaactggca gattattgtg aagccaaata
    16381 cagcctgctt acccagcaca ccgatcctga aaaagcgctg ccaccgctgc tggtgcgcca
    16441 gcaaaaagcg gtcgatttaa ttactcagtg ctatcagcaa atgcatatgc tttccgcgca
    16501 aaacaatacc gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga
    16561 acatatttcg gtcagtttgc atcagccgga agaggtgcaa aaactggtcg agcgtagcca
    16621 tgcggaagaa gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc
    16681 tgatgacatt ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc
    16741 actggaaaaa atcgcccgcc agcatccgga taatccggtt gggcaattct gctactggca
    16801 tttcagccgc atcgcccgcg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact
    16861 ggccgataaa cagcggcgaa tgccgttgct tccggcactg aaaagttatc tgtcactaaa
    16921 gtctccggcg ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat
    16981 gggcaccgtg ctgcatctgc cgaagtcgta ctggatcctg atgacggtat tgctggtgac
    17041 acaaaatggc tatggcgcaa cccgtctgag gattgtgaat cgctccgtgg gaaccgtggt
    17101 cgggttaatc attgcgggcg tagcgctgca ctttaaaatt cccgaaggtt acaccctgac
    17161 gttgatgctg attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc
    17221 aacggtcggt tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga
    17281 gcagtacatc cttccacgtc ttatcgatac cattattggt tgtttgattg ctttcggtgg
    17341 taccgtctgg ctgtggccgc agtggcagag cgggttattg cgtaaaaacg cccatgatgc
    17401 tttagaagcc tatcaggaag cgattcgctt gattctgagc gaggacccgc aaccaacgcc
    17461 actggcctgg cagagaatgc gggtaaatca ggcgcataac actctgtata actcattgaa
    17521 tcaggcgatg caggaaccgg cattcaacag ccattatctg gcagatatga aactgtgggt
    17581 aacgcacagc cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca
    17641 ccgggcattg ccacctgaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca
    17701 gcgttgtcag cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat
    17761 ggatgcgccg gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca
    17821 gcgggttatt ggccatctga acaccatgca caccatttcg tcgatggcat ggcgtcagcg
    17881 accgcatcac gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct
    17941 aaaccatcgg ataaggcatt cacgccgcat ccgacatatt ttgcctgatg cgtcttattt
    18001 acgccccaac caccttcgcc accgcgtggg cgaaacgttg catcccctca tcgatatccg
    18061 catcttccac caccagcgac ggtgctaaac gcatcacatc cggtccggca ttcagcacca
    18121 ttacgccaac ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tagtgtggtt
    18181 tcagctccgc gccaattaac agccccatac cgcgaatatc gctaaacaca tcgtactgct
    18241 gatcgatctt ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaatg ccttccagca
    18301 cttcaggggt attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac
    18361 cgccgtaggt ggaaccgtga gaaccaggat gaaacgcaga agcaatttcc gccgtggtca
    18421 gcatggcgct aatcgggaag ccgccgccta acgctttcgc agaagtcaga atatccggcg
    18481 tcacaccgta gtacctgtaa gcaaacaaat cgccagtccg ccccatcccg cactgcactt
    18541 catcaaacac caataatacc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact
    18601 ctggcgtcgc tgccgtcaca ccgccctcgc cctggatcgg ctcaaccacc accgcacagg
    18661 tgtgatcatc catcaccgct ttcactgcat ggagatcgtt aaagggaacg tggatgatgt
    18721 ctgccggttg cggcccaaag ccgtcggaat attttggctg cccacccacc gaaacggtaa
    18781 acagcgagcg accatgaaaa gcgttatgga aggcaataat tttggttttg aacgggctat
    18841 gacgcacaca ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg
    18901 agttcataaa caccacgcgt tcggcaaacg tcgcctcaat cagtttgcgc ccaagacgca
    18961 gcgccggttc attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta
    19021 acgcgttcac cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga
    19081 aatcgacata ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa
    19141 taaactctgc cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg
    19201 ctgtttgttc aattgccatc tcatgatcac cctgttacgc ataaataaat atgaaattat
    19261 aatcacaaaa tatgcataaa aaatcactga atggcaatcg gaaatcagag atgcaggaaa
    19321 ttagccagca gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ctgcacacct
    19381 tccagatccc actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc
    19441 acgtcaaagc acgctggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg
    19501 gtaagtggat ttgccagccc ccgaaatacg ccctcaccgc tatgtgtaat cggcgaggtt
    19561 ttgccgtgca tgacctttgc ggcgcgcaca actttgccgc caaatgcctg cgccattgcc
    19621 tgatgaccga ggcagacgcc aagaatcggc aagcgcccgg catagtggcg aataacgtca
    19681 agggagatcc cggcttcatc tggcgtacag gggccaggtg agatgacaat tttttgtggt
    19741 ttaagggcgt cgatatccgc cagcgtcaac gcatcgttgc gcttaaccag cacatccgcc
    19801 cccagttcac aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atctataagc
    19861 aggatcatgg cggctccggt acgaaagaac cgcgctattt tactcagatt ccccggcttc
    19921 gcttaccact ttgctaaata tcatctgcag tgcggtgaga tcccccattg ccccactctg
    19981 atttgcctga ttccaggcct ctttttcgat accctgccag ctcagttgat atccggcatg
    20041 aatcgccagt tgctcgaaga agatccgctg tgccagacca cttcccaccc ggaagggatg
    20101 cagcacgtcg atttcacaat agtaatgcgc cagccgctcg acgaacttcg ctttctccag
    20161 gccaaccaga taaccttctt cctccagatc ctgcatcagg gcattgccct ctttttcgat
    20221 ataagcaaag tggcagaacg gcgtatcgcc ctgataaata tcaacttcac gcagttgccc
    20281 tgcccagtcg aaaatatcct ggtacagctg gcgatggata gtgcgcaaat gcggtaaacc
    20341 gcgcaccagc ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc
    20401 ctgttccagc cgctgctgct ggcggatgtt cagccggtta cgcatgatat caaggccagg
    20461 ataaagatac ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagctctt
    20521 caaggcgcgc taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa
    20581 gacggcgact ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct
    20641 tatcggtgag tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag
    20701 cggcaaatcc gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa
    20761 tcattacggc aggactttag cggaaaggat aactaccggt tttgacggca cattctggta
    20821 cggaccaaca tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac
    20881 cactttacca aataccgcgt aaccgaaatc acgctgacca tggtcaagga aggcgttatc
    20941 ggcaacgttg ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc
    21001 gatggtgcca cgcgtgttgc gcaggccgtt atcggcttca tttttgattg gcgggtttgg
    21061 ttttttctgc tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac
    21121 gcggtgaaag gtagtgttgt tataaaaacc gctgttcaca taatcgacaa agttttgcac
    21181 agacactggc gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa
    21241 caatacgtgc gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa
    21301 aacagcagcc atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca
    21361 gaaaaaaagc aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct
    21421 aattttacgt atctgaaata tatgtaacct gacgatcact ttttattccg tgatcaaaat
    21481 cacctcttaa aatgcaattt agcaaccgat tgcaataaaa catttaaaca gatcacaaaa
    21541 tcacctaaaa tcgcccgctc gcggcatatc ccgctatgcc attttatttt tgaacgcagg
    21601 ccaatcatga ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ctcctacgca
    21661 ctgactggtg cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc
    21721 aatctgcctg tttccagtat gagtaacacc ttcaccttcc tcaacgccgg cattttaatc
    21781 tctatcttcc tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt
    21841 ggctttctcc tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg
    21901 ttctcgacgg cgatgttcat tctcggggtg gtcagcggca ttactatgtc gattggtaca
    21961 ttcctgataa cacaaatgta tgaagggcgt cagcgcggtt cccgcctgtt atttactgac
    22021 tccttcttca gtatggctgg gatgattttc ccaatgatcg ccgcgtttct actggcgcgc
    22081 agcattgagt ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt
    22141 ctgaccttca gctgtgagtt cccggcactg ggcaaacgtg caccaaaaac ggatgctccg
    22201 gtagagaaag aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac
    22261 atcctcggtc agttaggttt tatctcctgg gtgcctgagt atgccaaagg cctgggcatg
    22321 agcctgaacg acgcgggcac gctggtgagt aacttctgga tgtcatacat ggtcggcatg
    22381 tgggcgttca gctttattct tcgcttcttt gatttgcaac gcattctgac cgtactggct
    22441 ggtctggctg cgattctgat gtacgtcttt aacaccggaa caccagcaca tatggcgtgg
    22501 tcaattctcg ctctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt
    22561 tcacagcaga ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcggaacc
    22621 atcggtacta tgttgacctt tgtggttacc ggcccgattg ttgaacatag cggtccgcag
    22681 gcggcactgc ttacagcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta
    22741 ggtttcgtca gccgtcaccg tcagcataac accctgacct ctcattaatt gctaatgccg
    22801 gacggcacta tcgtcgtccg gccttttcct ctcttccccc gctacgtgca tctatttcta
    22861 taaacccgtt cattttgtcc attttttgca caaacatgaa atgtcagaca attccgtgac
    22921 ttaagaaaat ttatacaaat cagcaacata ccccttaagg agtatataaa ggtgaatttg
    22981 atttacatca ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc
    23041 gaggcaaaaa tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc
    23101 tttatcgaag atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt
    23161 gaagaaccgc gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc
    23221 gccgaagagc tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg
    23281 gtcggcgaac gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga
    23341 cgtaccgttt tttatgacaa gctgatcatg gcaaccggtt cctacccgtg gatcccacca
    23401 atcaaaggtt ctgatactca ggactgcttt gtctatcgca ccattgaaga cctcaacgcc
    23461 attgaatcct gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt
    23521 ctggaagccg ctggcgcgct gaaaaactta ggtattgaaa cccacgttat cgaatttgcc
    23581 cctatgctga tggcggaaca gcttgatcag atgggtggcg agcagctgcg tcgcaaaatc
    23641 gaaagtatgg gcgtgcgcgt tcactccagc aaaaacaccc ttgagattgt gcaggaaggt
    23701 gtcgaagcgc gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc
    23761 gtcttctcta ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt
    23821 gctccgcgtg gggggattgt cattaatgat tcctgccaga cttccgatcc ggatatctac
    23881 gccatcggtg aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac
    23941 aaaatggcgc aggtcgccgt tgaccatatt ctgggtagcg aaaacgcctt tgaaggtgct
    24001 gaccttagcg ccaagctgaa actgctgggc gtagacgtag gtggtattgg tgatgcgcac
    24061 ggtcgcacgc ctggcgcacg tagctatgtt tacctcgacg aaagtaaaga gatctacaaa
    24121 cgcctgattg tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc
    24181 agcgactacg gcaacctgct gcaactggtg ctgaacgcta tcgagctgcc ggaaaacccg
    24241 gattctctga ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa
    24301 ctgccggaca gcgcgcaaat ctgctcctgc ttcgacgtca ctaaaggtga tctgattgct
    24361 gccatcaaca aaggctgcca cacagttgcg gcgctgaaag ctgaaaccaa agcgggtact
    24421 ggctgcggtg gctgtatccc gctggtcact caggtactga acgcggaact ggcgaaacag
    24481 ggcatcgaag ttaacaacaa cctgtgcgaa cactttgctt attcgcgtca ggaactgttc
    24541 catttgatcc gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa
    24601 ggctacggtt gtgaagtttg taaaccaacc gtcggttcgc tgctggcctc ctgctggaac
    24661 gaatacattc tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct
    24721 aacatccaga aagacggcac ctactcggtg atcccgcgtt ctccgggcgg tgaaatcacc
    24781 ccggaagggc tgatggcagt gggtcgtatc gcgcgtaaat ttaatctcta caccaaaatc
    24841 accggctccc agcgcctggc gatgtttggc gcacagaaag acgatctgcc ggaaatctgg
    24901 cgtcagctga ttgaagctgg cttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg
    24961 gcgaaaacct gcgtgggtag cacctggtgc cgctacggtg ttggcgatag cgtcggcctc
    25021 ggcgtggaac tggaaaaccg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt
    25081 gtctccggct gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc
    25141 actgaaaaag gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg
    25201 gatctgctgg cagcggatat cgatcgcgaa acgctgatca aatatctcga ccgcttcatg
    25261 atgttctaca tccgtaccgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc
    25321 gaaggcggca tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgct
    25381 catctggaag aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg
    25441 gtcaatacac cgtccgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac
    25501 ccgaacgtgc agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt
    25561 atcccggtaa ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg
    25621 atgacatcct gcctgaaacc ggcgtctgcg cgctgttagg tgacgagcag gtagcgattt
    25681 tccgcccgta tcacagcgac caggtgtttg cgatcagcaa cattgatccg ttcttcgagt
    25741 ccagcgtgct atcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc
    25801 cgctgaaaaa acagcgtttt cgcttaagtg atggcttgtg catggaagac gaacagtttt
    25861 ccgtcaaaca ttacgaagca cgagtgaaag acggcgtggt gcagctgcgc ggttaatgtt
    25921 ttaacgggag gcgcaatgcc tccccttttt gcatggtcct gtaatgacct tcggtatatt
    25981 gcaggacatt ttttaaactt tttgttttat tttttgtttt tattttttaa aggataatca
    26041 aatgtttaca gacactatta ataagtgtgc ggctaatgct gcgcgcattg cacgcctgtc
    26101 ggcaaataac ccgctcggct tttgggtcag ctccgccatg gcgggcgcat atgtgggtct
    26161 tgggatcatc ctgattttca cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt
    26221 gatgggcgcg acctttggta tcgccttaac gctggtgatt atcgccggtt ctgaactgtt
    26281 caccggacac accatgttcc tcacctttgg ggtaaaagcg ggcagcatca gccacgggca
    26341 aatgtgggca atcctgccgc aaacctggct gggtaacctg gtcggttccg tcttcgttgc
    26401 catgctctat agctggggcg gcggtagcct gctgccggta gataccagca tcgttcactc
    26461 cgtcgcgctg gctaaaacca ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg
    26521 taactggctg gtttgcctgg caatctggat agcgctgcgc actgaagggg cggcgaaatt
    26581 tatcgctatc tggtggtgtc tgctggcatt tatcgcgtcc ggctacgagc actctatcgc
    26641 taacatgacg ctgttcgcgc tctcctggtt cggcaaccac agcgaagcct acacgctggc
    26701 gggtattggt cataacctgc tgtgggtgac gctgggtaat actttatcag gtgccgtatt
    26761 catgggattg ggttattggt atgctacgcc gaaagcgaat cgtccggttg cggacaaatt
    26821 taatcaaact gaaacggctg ccggttaatt actaaggggt ttttacgtgg atcatttgcc
    26881 tatattttgc caattacgcg atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga
    26941 acgcaaagca aggttgctgt tagacgcagg cgctcgctta acggtgaatg cattagcgtt
    27001 tattccacag ttcaccgcat gggcagatgc aggcatgtta accctcgtcg aagggccatt
    27061 tgatgaaagc cttctcgaca cctgctggct ggcgattgca gcgacggatg atgacacgct
    27121 taaccagcgc gtcagcgaag ccgctgaagc tcgtcgcatc ttctgtaacg tagtcgatgc
    27181 gccgaaagcc gccagcttta ttatgccgtc gattattgac cgctcaccgc taatggtagc
    27241 ggtctcctct ggcggcacct ctccggttct ggctcgcctg ttgcgcgaaa aactcgaatc
    27301 actgctgccg ttgcatctgg gccaggtggc gaaatatgcc gggcaattac gcgggcgagt
    27361 gaaacaacag ttcgccacga tgagtgagcg tcgccgtttc tgggagaaat tgttcgttaa
    27421 cgaccgcctg gcgcagtcgc tggcaaacaa cgatcagaaa gccattactg aaacgaccga
    27481 acagttaatc aacgaaccgc tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc
    27541 gggcgatgcc gggctgctga cgctgaaagg actgcaacaa attcagcagg cagatgtggt
    27601 ggtctacgac cgtctggttt ctgacgatat tatgaatctg gtacgccgcg atgctgatcg
    27661 cgttttcgtc ggcaaacgcg cgggatacca ctgtgtaccg caggaagaga ttaaccagat
    27721 cctgctgcgg gaagcgcaaa aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt
    27781 tatttttggc cgtggtggcg aagagctgga aacactgtgc aacgcgggta ttccgttctc
    27841 ggtggttccg ggtattaccg cagcttctgg ttgctctgcc tattcgggta ttccactcac
    27901 gcatcgcgat tatgcccaga gcgtacgctt aattaccgga cacttaaaaa ccggtggcga
    27961 gctggactgg gaaaacctgg cggcagaaaa acagacgctg gtgttctata tggggttgaa
    28021 tcaggccgcg actattcagc aaaagctgat tgaacacggt atgcctggcg aaatgccggt
    28081 ggcaattgtc gaaaacggaa cggcagtcac gcagcgcgtg attgacggta cgctcacgca
    28141 actcggtgaa cttgctcagc aaatgaacag tccatcgcta attattattg gtcgggttgt
    28201 tggcctgcgc gataaactga actggttctc caatcattaa tttaacccgg gccagagaat
    28261 tctggccttc ttaatgactc tttttatata ttcagcaaat aaaacataac cccttaataa
    28321 atattatctg ctaatgggtt atattcaccg ttacagcgac ttacaaaatt aagtcattcg
    28381 ttcactgacg ccaattaaat attcctgtat cgttcttaac gagcagtctg cttatattca
    28441 tataatcaat gaatattaat taatatataa tacataggga atgtaaatga acaaatttat
    28501 taaagttgca ctggtaggtg cagtactggc tacgttaact gcatgtactg gtcatattga
    28561 aaaccgtgat aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa
    28621 aatcattggc ggttgcggtc ctactgcaca gtaagtctct gacagaaatc ggctaacacc
    28681 ggtttaattg cccgacgccc tctggatttt ccgagggcgt atttgtttct atgtccttct
    28741 tgcccccgcc cgtaaaaata ttttcacttt aaattcaatt ttataactac atccattagc
    28801 cacagcgcga tcctgtgcga aattttgtga tcttcctcca cattacacaa catcatatgt
    28861 tgttatattc atcatgcatt gtcatgttgc cttttaaatg actgcaaact ctcccctaca
    28921 acgtattgga caagaaaaag gtatcgctat gggaagccag gaactccaac gcaagctcgg
    28981 attttgggcc gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc
    29041 tgtgggtgaa gtggcaaaag cagcgggcac gccgtagctt acggtgctcg cgtttgtcat
    29101 tggcgggtta attgtgatcc cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc
    29161 ggaaaatggc gcagattatg tttatctgaa aaatgccgga agccgaccgc tggctttcct
    29221 ctccggctgg gccagcttct gggccaacga tgcgccgtca ttgtcgatta tggcgctggc
    29281 gattgtcagc aatcttggct ttttaacgcc tatcgatccg ttgctcggta aatttatcgc
    29341 cgccggatta attatcgcct ttatgttgct acacctgcgc tccgttgaag gcggcgcagc
    29401 gtttcagacg ctaattacca tcgccaaaat tatcccgttc actatcgtca ttggccttgg
    29461 gatcttctgg tttaaagcgg agaattttgc cgcccttacc accactgcga ttggcgcaac
    29521 gggcagcttt atggcgctgc tggcggggat ctctgccacc agttggtcgt ataccggcat
    29581 ggcctctatc tgttatatga ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc
    29641 gctgattggt tcctgtctgc tggttctggt gctctacacc ctgctggcgc tggtgatttc
    29701 cggcctgatg cccttcgaca aactcgccaa ttctgaaacg ccgatttccg acgccctgac
    29761 ctagatcccc gcactcggca gcaccgctgg gatctttgtt gccatcacgg cgatgatcgt
    29821 cattcttggt tcgctttcca gctgcgtgat gtaccagccg cggctggaat acgcgatggc
    29881 gaaagacaac ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca acacgccgga
    29941 tgtctccatc atcctgcaag gggcgctggg gatcttcttc atcttcgttt ccgatctcac
    30001 cagcctgctg ggttatttca ccctggtgat gtgtttcaaa aataccctca ccttcggctc
    30061 catcatctgg tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc cggctttcgg
    30121 gctgatgacc accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg
    30181 ggcaccgatt cccggcctta tctgcgccgt catcgttatt gctactggtc tgcctgctta
    30241 cgccttctgg gcgaagcgta gccgccagct caacgctttg tcgtaatgtt atctgaagaa
    30301 aataaaatgt tggatattga taaaagcacc gtggactttc tggtcaccga aaatatggtt
    30361 caggaagtgg aaaaagttct cagccatgac gttccgctgg tgcacgccat cgtggaagag
    30421 atggtgaagc gcgacattga tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg
    30481 gcgcaaacgg cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc
    30541 ggctgggagt tctgcgataa caccccgtat cgcctcgacg atcgttgcgc ggtgattggc
    30601 gtttctgact acggtaaaac ggaagaggta atcaaagcgc tggagctggg ccgggcctgc
    30661 ggcgcactca ctgcggcgtt caccaaacgc gcggacagcc cgattacctc ggcggcggaa
    30721 tttagcattg attatcaggc cgactgtatc tgggaaattc acctgctgct ctgctacagc
    30781 gtggtgctgg agatgatcac ccgcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac
    30841 gatctcaagc agttgccgaa tgcgctcggt catctggtac gcacctggga agaaaaaggc
    30901 cgccagcttg gtgaactggc cagccagtgg ccgatgattt ataccgttgc tgcgggtccg
    30961 ctgcgtccgc tgggttacaa agaaggcatt gtaacgctga tggaatttac ctggacgcac
    31021 ggctgcgtga ttgagagcgg agagttccgc catggcccgc tggagattgt cgaaccgggc
    31081 gttccgttcc tgttcctgct cggcaatgat gaaagtcgcc acaccaccga acgcgccatt
    31141 aactttgtta aacagcgtac tgacaacgtg atcgtcatcg attacgccga aatttcgcaa
    31201 gggctgcacc cgtggctggc accgttcctg atgttcgtgc caatggagtg gctctgctac
    31261 tacctgtcta tttacaaaga tcacaacccg gatgaacgcc gctattacgg tggtctggtg
    31321 gaatattaat ccctctcccg gcccggcaac gggccggatt tacgcaagga gttacccgat
    31381 gaaaacaggt atgtttacct gcggccacca gcggctgcct attgaacacg catttcgtga
    31441 tgcaagcgag ctgggttacg acggcatcga aatttggggc ggtcgcccgc acgcgttcgc
    31501 gccggactta aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat
    31561 gccgattatc ggttacacgc cagaaaccaa cggctatccg tataacatga tgctgggcga
    31621 tgaacatatg cgtcgtgaaa gcctggacat gatcaagctg gcgatatata tggcaaaaga
    31681 gatgaacgcg ggttatacgc tgatttctgc ggcccacgcg ggctatctca cgccgcctaa
    31741 cgttatctgg ggcggctggc agagaactta agcgaactgt gtgagtacgc ggaaaacatc
    31801 ggcatggatc tgatcctcga accgttaacg ccgtatgaat cgaacgtcgt gtgtaatgct
    31861 aatgatgtgc ttcatgcgct ggcgctggtg ccttcgccgc gcctgttcag catggtcgac
    31921 atttgcgcgc cgtatgtcca ggcggaaccg gtgatgagtt atttcgacaa actgggcgat
    31981 aaattacgtc atctgcatat tgtcgacagc gacggggcca gcgacacgca ttacattcct
    32041 ggagaaggca aaatgccgct gcgggaactg atgcgcgata ttattgatcg gggctatgag
    32101 ggttactgta cggtggagct ggtgacgatg tatatgaacg agcccagact ctatgcccgc
    32161 caggcgctgg aacgctttcg cgcgctgctg ccggaggatg agagatgaaa accctagcga
    32221 caatcggcga taactgcgtc gatatctacc cgcaactgaa taaagcgttt tctggcggta
    32281 atgcggtcaa tgtggcggtg tactgcactc gctacggcat acagccggga tgcattacct
    32341 gggtgggtga cgatgactac ggcacaaagc tgaagcagga tctcgcccgc atgggcgtcg
    32401 atatcagcca tgtccatacg aaacacggcg ttaccgcaca aactcaggtg gaactgcacg
    32461 acaatgatcg cgtttttggc gactacaccg aaggcgtgat ggccgacttt gccctgagtg
    32521 aagaggatta cgcctggctg gcgcagtatg acattgtgca cgcggcaatc tggggacatg
    32581 cggaagacgc attcccacag ctgcacgctg cgggcaaact taccgctttc gacttctccg
    32641 acaagtggga cagcccgctc tggcagacac tggtgccgca tctcgatttt gcctttgcct
    32701 ccgcaccgca agaagacgaa gcgctgcgtc tgaagatgaa agcgattgtt gcccgtggcg
    32761 caggaacagt gattgtcacg ctgggtgaaa acggcagcat tgcctgggat ggcgcgcagt
    32821 tctggcgtca ggctcctgaa ccggtgacgg ttatcgacac catgggtgcc ggagattcgt
    32881 tcattgccgg attcctttgc ggctggtctg cggggatgac attaccgcag gcgatggcgc
    32941 agggaacggc gtgcgcggcg aaaaccattc agtaccacgg tgcctggtag gtataacgtt
    33001 ggcgtgagca tcttcacgcc aacgtgctgt tacttgccgg aaaacgaccc tataatccga
    33061 gtaattcatt ctttatttca gggtcgatta tgtcagctac ggaccgctac tctcatcaac
    33121 tcctctacgc taccgtccgc cagcggctgc tggatgatat cgcgcagggg gtttaccagg
    33181 ccgggcaaca gatccctacc gaaaacgagc tttgtacaca atataacgtc agccgcatta
    33241 ccattcgcaa agccatcagc gacttagtgg cagacggcgt actgatccgc tggcagggaa
    33301 aaggcacctt tgtacaaagc cagaaagttg aaaacgccct gcttactgtc agtggtttta
    33361 ccgattttgg cgtctcacaa ggcaaggcga cgaaagagaa agtgatcgaa caggaacggg
    33421 tcagcgccgc gccgttttgc gaaaagctga acatccccgg aaacagcgaa gtgttccatc
    33481 tctgccgggt gatgtatctc gataaagagc cgctgtttat tgatagttca tggatcccgc
    33541 tgtcgcgtta tcctgacttt gatgagattt acgtcgaagg aagctccacc tatcagttat
    33601 ttcaggagcg ttttgacacg cgagtggtca gcgacaaaaa gaccatcgat atctttgccg
    33661 ccacccgccc gcaggcaaaa tggctgaaat gcgaactggg cgaaccgttg tttcgcatca
    33721 gcaaaatcgc ctttgatcag aatgacaaac cggtgcacgt ctccgaactc ttctgccgcg
    33781 ccaatcgcat caccttaacg attgataata aaagacatta accgtaggcc ggataagatg
    33841 cgccagcatc gcatccggcg atgctggcgc actgaatttt acatcccgca cgttcccctc
    33901 accctaaccc tctccccaaa ggggcgaggg gagcgttcag tgcggaattt agcttgattg
    33961 ccgaacggtg ctatccagca ttaaatactc gcaatactct cacgcaaaat ccgcagcacc
    34021 gtctcttcac cgctgcgatt cgggttaatg cgaatcgcac aatgttcgag ttgtggattc
    34081 gcctggcgaa acgttcctga aagtcgatag aagagcggcg ggatttcata tttcgactct
    34141 gcaccgaccg ggtaaggcaa cgcgccacgc ttttgcgctt cttccagcac cctggcggca
    34201 atcagctgat gaaactcgac aatcaacacc ttcgactgcg cattggcaat gacggcgctt
    34261 ttcacttccg ccaccgcgcc gccgttaagc aaagccagca accgttcgga tactcccgcc
    34321 tgcaccgcat gcatcactgg cgcgaacacc agaccacgca agacttccag cgcctgtgag
    34381 ccctggatct ggctaccgcc ggagtaaagc gtggcgcgaa tccgactaat aacatcggca
    34441 tcgccaacca ctgcaccaac gccctccggc ccaaacagct tgaagcagga gaatgtcgag
    34501 acattcgcgc cgcattcaca gccgattcgc gctaccttca tcaccgcata gttatcatcg
    34561 gttaacgctg gaacacctgc cgcgcgcaac gttgccagca catctgccag cacatagctg
    34621 tcctgcggct gctggcgcgt atgctgcacc agcgcggcat caggttgttg ctcgtcgacg
    34681 acctgcttca gtgccaacag gtcattgaaa tcagcagtaa taagcgtcag ccccatctgc
    34741 tcaataataa cccgtgtcgt cgggtaaaca ggcgcgtcat gcaccagaag acgctgcccc
    34801 ggtttgagca aagccgccag cccggcgcga atcgcgccag tccccgcgcc ctgcaccagc
    34861 gccgcggcct gtgcgtgaaa tgcatcagcc agcacctgct ccacccgttg ggtaatacgc
    34921 ggttgattca gacctggagt taaccctaaa tcaccgccag caagaaactc gctgcctgga
    34981 aaatggcggc aaatagtgtc caccagcgca aactgctttt gctgcgcttc agcaagagtc
    35041 aggctttgca aaggaaacgt cttcatagga gccttcctta agccggaacg aacaggccaa
    35101 gccagtaaag aacattcagc agaataccgg tgatcattac tgccacgacc ggtgccgcca
    35161 ttttctgtac cggacggccc agggattcgt tgaggaagta aatggcaacc gcgatagaga
    35221 atccggtata acccgccatc ttaattgctg cgaaaatcga accgaccagc agcgccactt
    35281 ccatcagcat attcatggcg ttacggatgt tatccgacgc attacgcacc gacgggtagc
    35341 gtcccagcca tttgccgatc gaacgaagca gcaagacttc cgccgaaata accactgcgc
    35401 ctaataccgc tgcaaccatc ggattcggcg agagatagcc caccgcataa acaaaggtaa
    35461 agcccgcaac tgcatacaca cccgttgcta aggcggtggt ggcaatcaac ggcacaaacc
    35521 ccagtccgcg cataaattct gccagagccg cctgattaat cagcgtttgc gattgttccg
    35581 gcgtcacgcc tgcggaatac gctttctcca gtgtgaagat cgaaacttcg ctaccagcaa
    35641 aaatcttcat actggcaacg gcggcaatca atgctcccac gatggcgata taaggtaagt
    35701 ttttgataat ccgcgacgtg cgctcttcaa acaccgaaag cccgctggca tcgatgtcat
    35761 tttcatcacg atgacgcagg tcgtgagtta tcgcgatccc cagcagcatc accatgccaa
    35821 taaagatttc aatggattca gggttaagat gtgggaaata gcgcacgact accacacggg
    35881 tcatcagaac aaccacggcg gcgaccagac tttgcttcca gccaaactgg tagaaaatcg
    35941 ccaccagcgg gaacagtgca aaagcagaaa ccaccggcga gcttaattcc ccgaggctac
    36001 ctaatacatc caccggcagc gcggtcagca gctggttaac tggcaacagg caagtaagga
    36061 tcaacacgcc ccagatagcg ccaagaccaa acgccatcag gctgtttatc gccagtacgc
    36121 cgaggatatc ggtaggaaga aagagtaacc atgcgttgag cagccaggtt ttcagggtaa
    36181 aagagatccc caccgaggcc acaaaaccaa tgctcagacc aaaagcgata ctccccgcct
    36241 cgcgacggtt catatagcct tcaatcagtt gcggcaggat cgggtggatg ccgtcatgaa
    36301 aaacagccgc cgagcgatgc gccagaagcg atgtcatacc cgtcaggcac gccaccacga
    36361 taatctgaat atacagatcc atagtcgtgc cttttatttc agatggttaa tcaacatcgg
    36421 aatcgcgtgt tcgacgtgct caacggaaag gccaaacgcc actttccctt cagcgatcat
    36481 tttggcgata tgctcgtctt tcgctttaat gcctggtttg gcaatggtgc aacttttgtt
    36541 atagccaatc accgcgatgg caatcgacaa tgccgcgccc gcaccggtat tacacgcgcc
    36601 aatgtaataa tctagttgcc cggatttcac cttcattgcc gcttccatgt cgttgtgaat
    36661 gaaaacttca aaacagccag gagccgtcgc ttcaatggtt tttttaatct gctcacgctg
    36721 taagcctgca acgccaatct ttttcatgag tctgtcctta ttggaaaaat tgagagggat
    36781 tttcacgtaa catcacatcc acatcggcct gactgaatcc tgactggcac aattgcggaa
    36841 taaaggtggt caataaatag tcatagccat aaccaccatt ggcttttaaa tgggagcggc
    36901 gcgtaatatc catcgacagc atgacgcggt tcagcaaccc acggtcacgt agcgcgtgga
    36961 gcatcgcaat gcgcttttcg tccgggtagt aactgttctt accaatggtg tcgaactgca
    37021 cgtacgcgcc gagatcgatc atcttcaaaa tgttgtcgag gttgtctttc agatcgcagt
    37081 gaccaacggt gacgcgcgaa agatcaactc cgtgggcttg caacagagcc agttgttcca
    37141 gccccatggt gctgaacgac gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca
    37201 gcgcagcggc aataaatacc ttctcttcca gcggcgtaat ctttccttcg ctggagccga
    37261 tctccgcgat gatcccggct ttcagctccg tgccatcgat accctgttca atttcatcga
    37321 ccatctcctg caccagttcc tgcacgctgc gggtcaccac atgttccggg aaaaacgcat
    37381 cctggtaata accagtacag gccaccacgt tgatcccggt ttcgcgcatt acatcgagca
    37441 taaattgcgc attgcgcccc atgtaacggt tggtcatctc aatcacatta cgtacacccc
    37501 gggtcatcag gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca
    37561 cgttgttttt aaagccggag agatcaatat gcagatgctc atgggccagg gtgtaacccg
    37621 tcggatcaaa actcataggg tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc
    37681 attttgtggc ggtggcgcac ggaaaaattc acacacggta gctcccacat cagaaagcgt
    37741 ggtgcgcaca ccgagctgcg tggcgaccag cccttgctga taaaccagca ctggcaccac
    37801 ttcacgggta tggtggctgt gaccaatggt cggatcgtta ccgtgatccg ccatcacgac
    37861 caggcaatca tctggctgca tcgcctcaac aagccgggca aggttacggt caacgacctg
    37921 caaacgttcg gcataacgtg cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat
    37981 attgatgcaa ataaacgccg tcggatgggt gttaaattcg tcgagggtga tatccataat
    38041 ccgctggcta tccaccaggt tttgccagct cacaccataa ggattgctga cgatatctgc
    38101 caccttaccc accagcacgg ttggcacgcc cgcttcatac agtttttgcg gcacctgcac
    38161 tttttcatca acaccgtagc ccatatgcac gacctggaag ccgttgtcat aagcgccaga
    38221 acgcggcgca ttaataccaa taaagcgccc ttctttgctt tctgcggcat cgagaatacg
    38281 ctgactgttg gttaacaggc cgccaaatgt aatgacccga ccgacctgta cctggtcacg
    38341 cacgatacga ccaattttga ttgcgtcgtc aaaagagatc acagagagat tggcggtaat
    38401 gttatagact tggcctaaat ccgcctcgag attatcgcca atcgcaaccg cctgattgac
    38461 ccacagaaat tgcagatcat cgccacggcg ctccacctgc cagccagcgg aaactaatgc
    38521 ctgctcaaca cggtcaatca catcgcgaaa aggcatccgc agcggcggta acgggcgcgt
    38581 gcctaaaatt tcctgatgcc ccataaaggt atcgccacct tcatgttgca gctctgccac
    38641 gccccaggtt gcggaatctg acggctgcat atcgcctggc gcataaccca atgtgttgat
    38701 tagccccagc ttctccaacg ttggtagctg caaatgcggc aactggctca ggatgtgacc
    38761 acaggtattc gccccggcat cttgcggacg caccagcgtg acatctttca ttgcccctac
    38821 gccaaagcta tcaatcacta acaccacaaa tcgcgccatt tatgccttca gagggttgcc
    38881 gaggctgtca taacgaccaa ctatttctgg ttcgccgcgc tgaataccgg acaccagcac
    38941 cacatcgcta cgggtgacaa aaatctgcgt gcgaaaacag agcaccactg cactgctcac
    39001 cggatactcg cctgccagcg gcagggtgta gtcgatactg ctgtcatcca cagttttgag
    39061 attggtttca gtaatctttt gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc
    39121 acgacggtag taaccgccgc cgtagcagta gctgtcgcca cggaaatgat gggagatttc
    39181 acttaaccag agcatcgcga tacgttcagg ctgatcgccc tgctggtttg ccggaatagt
    39241 accggtcagc gcatgaccgg gttcggcatg agtcgcaccg tattccgcca gcaatggcag
    39301 cgaagtgcag ctggtcgctg aaggtacgtt cagttgctca atcgcaatac cagatttcgc
    39361 cagttgatcc cgtgcttgta ccagcgtgtg aagattcggt gttggcaaaa ctttcccggc
    39421 agcctcatcc caaagcaggc aagggaaatg ggttagtccg gctaaatgca gccccggcag
    39481 tttcttaatt tcagcaatca cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc
    39541 cggataaaga aaatcatcgt cgctataaac tttgagcagc acaggctgga cccgtccggc
    39601 cttaaccgct gccgcagaaa cttcccgcgc tttgtcgaga gtaaacacgg tgatgatgtc
    39661 ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg atttgtacca gatgcccctg
    39721 atgcgccact ggcaaaccag cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac
    39781 aatgccgctg tagcctaatg ccagcaattt ttccgccagc cacggattac gaccaaattg
    39841 cttggtcatc agatacagtt caatgccgta aagccgcgcc gtctcaatga gccgcttacc
    39901 gttttccagt atctgatcca cgtcgatcac ccagctgtcc ggggcgattt tgccctgctg
    39961 ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa
    40021 catcttgttg cctcgtttct gttccttgaa ttaaatattc atttttttaa atatttaggt
    40081 tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt tagcagccat ccataatcca
    40141 taaagattgg ccagaaaata gccttcttca cacgggttaa cttccagagc gaattccttc
    40201 aacaacacct gatgcaattg cacaacggct tgccagtggc tggattgcgc cagttccgcc
    40261 agcaactcgt tatccagcgg ctctatttct tcaccacggc gactgcgcat cagtgcactc
    40321 gccatatgtg tcatcgccat cgttccttgc tcactgcgca ccggcagatg gcactctttt
    40381 tccagtacgt tgacgacctg catcatgcct ttgcagacgt ccttatcaat aacgcctgcc
    40441 tcgcaaagca ggttgagtct ggtttccata tcagatgcct ttcgtttaat aactgggttc
    40501 ctgttcccca ctcaccaccc gttgttgatg ggcaagcagg gcgtgtttat ctacaacggc
    40561 acgcagcaat tgttgcattg ggtaatcatc gactcgcgtc aggaccacgg cttcggtggc
    40621 cagtaaaaat cgcggatcgt ctgttagcgg cgtcgcctct aatcccagca tggtcagttc
    40681 gttttccgcc accacgttcc agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa
    40741 actctcgtga taagagagat cgactcgttc cacatcacta tcgccaaaaa agacatcggt
    40801 catgattttc tgatccgccg aacgactatc gagacctact cgcttaacat ttccagactc
    40861 acctttgcgg catatcagtt gatgttcacc aacatacgta tgcgggccaa gctcgagggc
    40921 gatgcacaaa ttgttttggc tgagataact ttccgccgcc aggcgggaaa ctactgccat
    40981 gtcatagaca ccgttaagta aacactccac gcgaatatcc gcgccgcgca tatgcgcata
    41041 gtaaaaagga atgccatcaa actgggcttt caatccgctc gctaaacctt cgtacaagcg
    41101 ggtatagggc aaaggcatcg cacataccac gttgttgata tccacatgac tcagcaatgc
    41161 tttgttatcc atctcgacaa gataactgcc attgcgccca cggcgttcaa tccgtatcgc
    41221 tccgcttgat tccagcgttt tcaacgcggc ctgcgtcagg ccaaccgatg aacggcattc
    41281 agttgccagc tcatctatgg ttttcagtcg attaccgcat ttttcaccca acaaataacg
    41341 ggccagtgtc gtgatgacga cgccttcttt tttgataaac gttcgacgca taatatattt
    41401 tcaataaaat gaatatttat atcttcatta aaatgaagat aagtggcgac gacgagatgt
    41461 ggaaacggcg aggcgcttca cattttttcg ggttgttgcg aaaagaggat aaaaaaaccg
    41521 ggtttccccg gtttcagagt gatgacgaag tgatttattc gacaatgcga gcaaagggta
    41581 attcccactg gcgcttgcct gctcccctca ccctaaccct ctccccaaag gggcgagggg
    41641 accgtccgag cgttttgata ttacggcttc gccacaaaac caatcgcttc gtacaccgct
    41701 ttcagcgtac gggaagcgtg tacactggct ttttctgcgc cgtctttcat cacctgttgc
    41761 aggaaggctt catcgttgcg gaaacggtga tagcgttcct gcaattcagt cagcataccg
    41821 gaaacggcat cagccacttc acctttcaga tgaccataca tcttgccttc gaactgtttt
    41881 tccagttctg ggatgctctg gcccgttacc gcagaaagga tatccagcag gttggaaacg
    41941 cccgctttgt tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgcg
    42001 cgtttgattt tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcgg
    42061 ttatcgtcag acttggacat cttcttggtc ggctccagca gcgacattac gcgcgcgcca
    42121 gatttcggaa taaacggctc cggcacctta aagatctcgc catacagcgc gttgaaacgc
    42181 tgggcgatat cgcgactcag ttccaggtgc tgtttctggt cttcacccac cggtaccaga
    42241 ttagtttgat acagcaggat gtccgcagcc atcagcaccg gatagtcaaa cagaccagcg
    42301 ttgatgttct cggcataacg cgcagattta tctttaaact gcgtcatgcg actcagttcg
    42361 ccgaagtagg tatagcagtt cagtgcccag cctaactgcg catgttctgg tacgtgggac
    42421 tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta
    42481 tccaacgtcg ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca
    42541 acgatacagt aaatgcaatg gtagtcatcc tgcatgttta cccactgacg cagcgcaccc
    42601 atgtagttac caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta
    42661 gtcatttttc gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt
    42721 tatagactga taaattacat caggctggct gagatcgata gcctcgccgt agttatatcc
    42781 gtaggttaag ccaactgatg ggcaacctgc cgcttttgcc gcctgaatat cattgcgtga
    42841 gtcgccgaca aacagcatct gttgtggggc aattcccatc cgctcagcca ccagtaacag
    42901 cgggtccgga tgcggttttt tgttttgcac atcatcgccg ccgataacca cgctaaagta
    42961 tttggcaata tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac
    43021 caggcctagc ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa cgtgcgggaa
    43081 caaaaacgtc ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcagaat
    43141 acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc ggtttcccca ttgttttacg
    43201 cagagtcgca cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa catctgcgcc
    43261 gttaccaatc caggtaataa cgcgttcttc acctgcgacg ggcaactcca gcgcatacag
    43321 cgccatatct accgcagcag caagaccagg agcactgtcg accagcgtac catcaagatc
    43381 aaaagcgacg ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc
    43441 gcatttcatc aatgactttt ttatagtctg gctggtcgaa gattgccgaa ccggcgacga
    43501 acatatccgc gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcca
    43561 cttccagacg aatgtcaaag ccagactcgt cgatacggcg acgtacttcg cgcagtttat
    43621 ccagtgtttg aggaatgaaa gactgaccgc cgaaaccagg gttgacggac atcagcagga
    43681 tcacatccag cttatccatc acgtaatcca gatagctcag aggtgtcgcc gggttaaata
    43741 ccagacccgc tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg
    43801 aggcttctgg atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc
    43861 gatcgacggg tttcaccatc agatgtacgt cgataggggc ggtaatgccg tagttacgca
    43921 aggatttcag caccattggc ccaatcgtca gattgggaac atagtggtta tccatgacgt
    43981 caaaatgcac gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg
    44041 caaaatcagc cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga
    44101 gaattatttt ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac
    44161 ctttttacgt gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt
    44221 tgcgcgctga taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat
    44281 atggcgctca accagacctt cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa
    44341 actgtttgtg tgatacgccg taaagttggc ggtcgcagac agcggtgcat aaggcggatc
    44401 gcaatagacg acggatgcat catctgcgcg ctccatgcta tcggcgtaag actcacaata
    44461 gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg
    44521 ttttttgtag cggccaaacg gcacgttaaa ctcaccgcgc agattgtaac gacacaggcc
    44581 gttgtaaccg tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct
    44641 tttgttgaac tcttcgcgga actgatagta aacctcggcg caatttgttt cgggaacaaa
    44701 cagctcgcgt gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact
    44761 gatcaggtcg ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac
    44821 cgacccggca cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat
    44881 atcatcaagc aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat
    44941 gccgactaac taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct
    45001 tcaggcagct cccgcaacga taaaccattg cgggagataa ccttgattac ttcagatcgg
    45061 cctgtacctg acgcagcggt ttcgcccacg ggtttttggc ctgaacatct gctggcaatg
    45121 tagataccgc ttttttcgcc tcttctttcg aagcgtacac gccagaaacc aggacatacc
    45181 acggctgacc attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg
    45241 cccaaccgtt caggttgtcg tagttagagg aactgctcag ctgcagagtg taatggctgg
    45301 acggtgccga tttcaacgaa ccaacattac ctgcggtctt ccctccagcg gctggtgttg
    45361 ccgtggtttg cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttggtg
    45421 ctggcgtaga agtcgcagcc ggtgccttcg tgctcgccac aggggcagct ggttcagtac
    45481 gcttcggcgt ctgtgctacc ggcttcggct ccgttttcac ggttgcttgc ggttttttcg
    45541 gttcaatcac cgcctgctga cgaactgggc gcgtagtgga cggacgttca gcggtttgcg
    45601 ttttcgccgt gtcacgcgat gcattgccat tgcgaacagg cgcaaccgtt gcgggttcag
    45661 tcggcaacgt ggaattgacc gccacattgt tcagctgttg ctgattttgt ggctgggtca
    45721 gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg accatccgtt gccaccgggg
    45781 tttgcccttg agtcggcgta gaagagatcg gtggcagaga aacatcctgc tgagtatttt
    45841 ccgcagacgt ggttcccggc gctggctgca cgccattcgc ctgatcggtc gcattgccag
    45901 caagatcaat actcttctcg ccagacgcgg tttgatcgct ggaagtggtc gagggggctt
    45961 ttagcgcaga accgataccg atgatcaaca gcagtagaac cagaatgccg acgcccatca
    46021 tcatatactg acgagaagcg ggtttactgg ctgctttttt gcgcttacgc ggacgacgct
    46081 ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgatttccg
    46141 gttcctcatt gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt
    46201 caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag
    46261 attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt
    46321 tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct
    46381 gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa
    46441 tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca
    46501 attgccaacg gaagaattaa gcgcatctct cccgcaagga ctttcttgtc acgcagcata
    46561 tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa cccagcccgc
    46621 gtgagcaggg ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac
    46681 gtccgcgccg ccatcaccat acccgcagcg accgcttcac catgtaacca attgccatac
    46741 cccatttcag cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac
    46801 ccggtttcgc gctcgtcggc ggcgacaact tctgccttca gttcacaaca acggcgaata
    46861 cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag
    46921 ttaaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct
    46981 aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga agcaggctgg
    47041 tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg
    47101 gaggaatcga cctgcgacag taacgtcgtc gggacttgaa tgaaacgaac accgcgctga
    47161 taactcgccg ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccagc
    47221 gtagtatcgc gaccatgcgg cttttgtaac aacgccgtaa agacggtatc gagtacagct
    47281 aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt
    47341 tcaagtacgc cgcggacctt atcgagatac agaggagcca gggtttcgct ggtgaccaac
    47401 atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat
    47461 gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga
    47521 catccacctt aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt
    47581 ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg
    47641 tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt
    47701 cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa
    47761 gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac
    47821 gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg
    47881 tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga
    47941 aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt
    48001 ccatattgag tttttgagct aactggcgcc caatagtgct ttttccggca cccataggcc
    48061 caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta
    48121 atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata
    48181 ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat
    48241 cacccatccc tctgcatatc tggtcgctgc aagcgcactg ccttgctacc accgctctgg
    48301 cgataaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa
    48361 atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta
    48421 tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcataaaaa cattaccgct
    48481 tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt
    48541 cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc
    48601 aacggtacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac
    48661 gtttctccgc tttttacctc gacctgcgtt tcgatctcct gcttatcaat cgccagcact
    48721 tcgccatcgg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc
    48781 cggatgcgac ctttttgtaa caccgtgggc gtgacctcca tccccaggac ggcctcttta
    48841 aattccaccg acgtcgcgcc actttccccg ctggaagcct gatatggaat ttcgctcccc
    48901 tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc
    48961 agctgctgtt tttgttcgag cgcggaaagc tcaagatcca gcaagcgtcc gttgatgcgc
    49021 ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct accaagcgtg
    49081 gtgacttgcc caacgccacc agcgtgttgc gcatcggcca gcgtccattt cacgcctaac
    49141 tcacgcaaac ttttttcatt aatggtgaca atatgcgccg acagctcaac ctgcccgacc
    49201 ggcagatcca tttgcgctac ccactgttca agcgcgctta acgccgtttt gttatctcgt
    49261 agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc
    49321 tcccccgctt tcgccagttc tcccgcgtcg gcgtattgca gggttatact gcgattttcc
    49381 agcggcagat ttgcctgcgc ccgcgcctgc tccgcctcct ggcgggcgat attgtcattc
    49441 tgccaggcaa tggaatgcac tgagagaatg ttgccttcct gccgcgttat cagtccggcg
    49501 cttttcacta cagtttgtag tgcctgcttc cagggaacat ctgttagatg taacgacacc
    49561 gtaccgctga cgtctggcga cacgaccagg ttcaacttct cctgttcagc cagcgcctgc
    49621 aacacctgag ctaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc
    49681 tgtacgccgg gtatcagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc
    49741 ttgccgttgc cacaaccatt gtggcggttc gcagtttgtc ccggtaccca gcgttagtac
    49801 gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc acatcgtttt gctgcacccg
    49861 tcgccatttc ttttgcccgt cttttattac accgatgatg cgctcgcctc gccctaccat
    49921 cccctgatag cgccactggc taagttcgct aatccggcat agatcttccg gcgatttaaa
    49981 agggtcacgc ataccggtta aaaggcacaa tgcaatacct gccaacaacc agcgtttaac
    50041 cctcatgcgg catctccagt tgtagcgtga acaaaagatc atcaccttcc acgcttaacg
    50101 aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt
    50161 cccacagcgt tttcaacgcc aactcgcctc cctgcgcgga tggatgccag gaaaccagtt
    50221 gcgcgccgga taactgaaaa tccagtggcg aaaagggcag cgttttttcc tcgctgaagg
    50281 gagtggtgtc taccaggcga tacagtgcgg cccactgctg gtgatgactt gcccgtagtc
    50341 gaattaatgc gtcgcgctct tcatggtgtg tcgatgatag aaaaatgagc gtaactaaca
    50401 tcagcaacca gaatgcccag caaaactggc ggaggcgggg tgatgtggcg aaccaccagt
    50461 caaagaacat gttcatcgct aaccttcctt gttaactgat actcaaattg ccagcgtccc
    50521 tgcgcatcct gctgcgtggc tccccgctga ttgagatgaa aagacgcatc ctggcggagt
    50581 gacgtttcta gtgcgtttaa cgcggtaatg cttgttgtca gccccttgat ctccagcgtt
    50641 ccctgctgcc agcttatcgt tgtcagccag gcgtgctcgg gtaaaagcgc cgccagtgct
    50701 tccagcgcag attgccagtc gcgggtaaat tgtcgctggc gctgccgttg cgaacgctgt
    50761 tcgcgtaact gctgttgctc cagcaaacgt ggcttcgtta tctgtaagct gcgggcgagt
    50821 tgttgttccg cctgaagcaa aactgcgtct atgcgcgctt cggcgctgcc tgtcagacgc
    50881 agtattagcg ttatcccgac ggccagcagc agaggcgcaa cgaacatcag caaccagaaa
    50941 cgcagaaaag cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc
    51001 agtacgcctt cccaagcgcc agccccagcg cgatggcaaa gtctccacca ggcggcggta
    51061 gcggcggctg acgaacagaa acggcctccc aggggtcaaa tccgccttcg ccacatatcg
    51121 cgacgctgtc gggatcaacg gataacgctg ccgccagctc cttcgcgcta gtcatcccca
    51181 ccgccagttt gcgcccccag ctatagcgcg tcgcccacag ccactgttcg ttatcacgcc
    51241 aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat
    51301 ccggggtgat cgcactaaca tgaacacgca acctttctgc cagcgtaagc agcgttgcca
    51361 gctctttgct ttgcgcggca gtcacgttat aagcggggct gagtgagtct tcgctgtaat
    51421 cgaagcgcag ggagtccgga tccatatcca gctcgcgggc catcgtccct gacagccagg
    51481 ccgtttgctc ccgctcacca agggacatcg acgggcgcgg aaatgaccgc tgtaatgtgc
    51541 gactggcggg aaacgccaac ataatgtgat gacgctgcgg cagttcgcga ctccacggta
    51601 acaacgtttt agccagctgc tgcgcatcaa caatccgccc atctttgata atgtcgtgcg
    51661 ccagcggcaa ccgccaccag cgttgcaaaa agcattcttt tgcgccccgc acgatcgcaa
    51721 ccgctgccgc ttcttgctgt tgtaaatgca aaccaatttg ccagatctta aatgtcattg
    51781 tgatgatctc cttatcaccc gtcactctga cgggtatatc aatgcgtctg gcttgccttt
    51841 atactaccgc gcgtttgttt ataaactgcc caaatgaaac taaatgggaa atttccagtg
    51901 aagttcgtaa agtatttttt gatccttgca gtctgttgca ttctgctggg agcaggctcg
    51961 atttatggcc tataccgcta catcgagcca caactgccgg atgtggtgac attaaaagat
    52021 gttcgcctgc aaattccgat gcagatttac agcgccgatg gcgagctgat tgctcaatac
    52081 ggtgagaaac gtcgtattcc ggttacgttg gatcaaatcc caccggagat ggtgaaagcc
    52141 tttatcgcga cggaagacag ccgcttctac gagcatcacg gcgttgaccc ggtggggatc
    52201 ttccgtgcgg caagtgtggc gctgttctcc ggccacgcgt cacaaggggc gagtaccatt
    52261 acccagcagc tggcgagaaa tttcttcctc agtccagaac gcacgctgat gcgtaagatt
    52321 aaggaagtct tcctcgcgat tcgcattgaa cagttgctga cgaaagacga aatcctcgag
    52381 ctttatctga acaagattta ccttggttac cgcgcctatg gtgtcggtgc tgcggcacaa
    52441 gtctatttcg gtaaaacggt cgaccaactg acgctgaacg aaatggcggt gatagccggg
    52501 ttgccgaaag cgccttccac ctttaatccg ctctactcaa tggatcgtgc cgtcgcgcgg
    52561 cgtaacgtcg tactgtcgcg gatgctggat gaagggtata tcacccagca acagttcgat
    52621 cagacacgca ctgaggcgat taacgctaat tatcacgcgc cggagattgc tttctctgcg
    52681 ccgtacctga gcgaaatggt gcgccaggag atgtataacc gttatggcga aagtgcctat
    52741 gaagacggtt atcgcattta caccaccatc acccgcaaag tgcagcaggc cgcgcagcag
    52801 gcggtacgta ataacgtgct ggactacgac atgcgccacg gctatcgcgg cccggcaaat
    52861 gtgctgtgga aagtgggcga gtcggcgtgg gataacaaca agattaccga tacgctgaag
    52921 gcgctgccga cctatggtcc gctgctgcct gccgcagtca ccagcgccaa tcctcaggga
    52981 gcgacggcga tgctggcgga cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg
    53041 gcgcgtcctt accgttcgga tactcagcag ggaccgacgc cgcgtaaagt gaccgatgtt
    53101 ctgcaaacgg gtcagcaaat ctgggttcgt caggttggcg atgcatggtg gctggcacaa
    53161 gtgccggaag tgaactcggc gctggtgtcg atcaatccgc aaaatggtgc cgttatggcg
    53221 ctggtcggtg gctttgattt caatcagagc aagtttaacc gcgccaccca ggcactgcgt
    53281 caggtgggtt ccaacatcaa accgttcctc tacaccgcgg cgatggataa aggtctgacg
    53341 ctggcaagta tgttgaacga tgtgccaatt tctcgctggg atgcaggtgc cggttctgac
    53401 tggcagccga agaactcacc accgcagtat gctggtccaa ttcgcttacg tcaggggctg
    53461 ggtcagtcga aaaacgtggt gatggtacgt gcaatgcggg cgatgggcgt cgactacgct
    53521 gcagaatatc tgcaacgctt cggcttcccg gcgcaaaaca ttgtccacac cgaatcgctg
    53581 gcgctgggtt cagcgtcctt caccccaatg caggtggcgc gcggctacgc ggtcatggcg
    53641 aacggcggct tcctagtgga cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc
    53701 gtgattttcg aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc agtgatttac
    53761 ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt cgctatctcc
    53821 cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta
    53881 gtggcgaaga ctggcgcgca ggagtacgca ccgcacgtca tcaacactcc gctggcattc
    53941 ctgattaaga gtgctttgaa caccaatatc tttggtgaac caggctggca gggtaccggc
    54001 tggcgtgcag gtcgtgattt gcagcgtcgc gatatcggcg ggaaaaccgg taccactaac
    54061 agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt
    54121 ggctttgatg atcaccgtcg taatctcggt catacaacgg cttccggagc gattaaagat
    54181 cagatctcag gttacgaagg cggtgccaag agtgcccagc ctgcatggga cgcttatatg
    54241 aaagccgttc ttgaaggtgt gccggagcag ccgctgacgc cgcctccggg tattgtgacg
    54301 gtgaatatcg atcgcagcac cgggcagtta gctaatggtg gcaacagccg cgaagagtat
    54361 ttcatcgaag gtacgcagcc gacacaacag gcggtgcacg aggtgggaac gaccattatc
    54421 gataatggtg aggcacagga attgttctga ttaaaaaggc gcttcggcgc cttttcagtt
    54481 ggctgacaaa gtgcatttgt ttatgccgga tgcgggtgaa cgcgttatcc ggctaacaaa
    54541 atcgtgaaaa atcaatatat tgcaggaacc ccataagcct gataagcgtt gtgcatcagg
    54601 caaacttcac gcatttacac tcgcccctgc cctttcaacc attcgcgcac gaggaacagc
    54661 gcgctgacat tacgcgcttc gttgaagtca gggtcttcca gcaaatccat catatgcgcc
    54721 agcggccagc gcacctgtgg tagcggctct ggctcatcgc cttccagcga ttccgggtag
    54781 agatcttgcg cgaccacgat attcatttta ctggaaaagt aagacggtgc catgctgagc
    54841 ttcttcaaaa aagtcagatc gttcgcacca aatccaacct cttcttttag ctcgcggtta
    54901 gcggcttcgt agacgctttc acccggatca attaatcctt tcgaaaaacc taattcgtag
    54961 gattcagttc ccactgcgta ttcgcggatc aggatcaggt gatcgtccac aatcggcaca
    55021 atcatcactg cttcccggtt ggttggacgc attcgttcat aaacacgccg cacgccattg
    55081 ctgaactcca gatccacact ctcgacggta aacagtcggg aacgggctac agtttcaaca
    55141 ttcagaatgg tgggtttttg taatgatttg ttcatcgtgg gatctatgct gtgaaatcag
    55201 cggttattgt gcgatatcgg acacgctttc ggcaatgtga attgcatgtt atttacattt
    55261 atgtaactta ataaataatc gtcctcaaat caaattaaaa gtcaataggt tgaaataact
    55321 ccaggaattt gctgatattc cgccttcaga gggtttgcta tgatcagcgg ttactgtgat
    55381 gtgcttaatg atgctcaagt taaactccac gcttgccgat agccaaccgc agaatcatgt
    55441 attgtgtccg gtgcgactga ccacgcctga cagactaagt aagatgggga aagcatgagc
    55501 accattgtga tttttttagc tgctttgctg gcctgctcac tacttgcggg atggctgata
    55561 aaagtgcgat ccagacagcg tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg
    55621 cgtaaactca cacctgaaga acgtagcgcc gttgaaaatt atcttgagag cctgacgcag
    55681 gtattacagg tgcctggccc aacgggagct agcgcggcac cgatctctct ggtgctgaat
    55741 gccgaaagca acaacgtcat gatgctgaca cacgctatca cacgttacgg catctctacc
    55801 gacgatccga ataaatggcg ttactacctc gattcggtag aagtccacct gccccccttc
    55861 tgggaacagt acatcaacga tgagaatacc gttgaactga ttcataccga ttcgctgccg
    55921 ttggttattt cactcaacgg tcatacgctg caggagtaca tgcaggaaac gcgcggctat
    55981 gccttgcaac ctgttccgtc cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc
    56041 gagctactca atattcgcaa agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg
    56101 ctgcgtgaag cgttgctgat cgtcgcctcc ttcctgatgt tctttttctg cctgattacc
    56161 ccggatgtat ttgttccgtg gctggcaggc ggcgcattac tgctgctggg cgcaggtctg
    56221 tgggggctat tcgcgccccc ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt
    56281 acaccccgtc gttggggatt gtttggcgaa aacgatcagg aacagatcaa caatatttca
    56341 ctcggtatta tcgacctggt ctatcccgca cactggcagc catacattgc tcaggatctc
    56401 ggtcaacaaa ccgatattga tatctatctc gaccgccacg tagtgcgtca ggggcgatat
    56461 ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc actggctgcg cagtacgatt
    56521 atcgctgcgg gctcgctgct ggtgctgttt atgctgttat tctggatccc gctggatatg
    56581 ccgctgaaat tcactctctc atggatgaaa ggcgcgcaga ccattgaagc caccagcgta
    56641 aaacaactgg ctgacgctgg cgtgcgggta ggcgatacat tgcgtattag cggtacggga
    56701 atgtgtaata ttcgcacttc cggaacctgg agcgcgaaaa ccaattcacc ttttttacca
    56761 tttgactgct cgcagatcat ctggaatgac gcccgctcat taccattacc agaatctgaa
    56821 ctggtcaaca aagcgacggc attgactgaa gcagttaatc gccagctgca ccctaaaccg
    56881 gaagatgaat ctcgcgtcag tgcctcatta cgttcagcga ttcaaaaatc cggcatggta
    56941 ttgcttgatg attttggcga cattgtactg aagacagcgg atttatgttc tgccaaagat
    57001 gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca acagtaaaga ctgggacgcg
    57061 ctggtaaaac gcgccaacgc tgggaagctc gatggcgtga atgtgttatt acgcccggtg
    57121 agtgcggaat cgctggataa cctggtggca acctccaccg cgccgtttat cacgcatgaa
    57181 acggcgcgag cggcacaatc actaaacagt ccggcccccg gcggattcct gattgtcagc
    57241 gacgaaggca gcgattttgt tgatcagccc tggccttcgg catcacttta cgactacccg
    57301 ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa tgctgatgca tacgccgttt
    57361 aacgccgaag gtatcgtcac aaaaatcttc actgacgcca atggtacgca gcatattggc
    57421 cttcatccga tcccggatcg ttccggcctg tggcgctatc tcagcaccac attgctgcta
    57481 ctgacgatgc tgggtagcgc catttacaat ggcgtacagg cctggcgtcg ttaccagcgt
    57541 catcgcactc gcatgatgga gattcaggcc tattatgaaa gctgcctgaa cccgcaactg
    57601 atcacccctt cagaaagcct tatcgaataa cacgtttgcg cggcaggtta tgctaccctg
    57661 tcgcgcaaat tgcttcactc tggagatttc cctcatgcat atcaacattg cctggcagga
    57721 cgtagatacc gttctgctgg atatggacgg cacgttgctc gacctcgcct tcgataacta
    57781 tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa aacggggtta cgccacagga
    57841 agcgatggaa tatatgcgcc agcaatatca cgacgtacag catacgctaa actggtactg
    57901 tcttgattac tggagtgagc aactgggtct ggatatctgt gcgatgacca ccgagatggg
    57961 accgcgcgcc gtactgcgtg aagataccat tccgtttctt gaggcactga aagccagcgg
    58021 taagcagcga attttgctca ccaatgctca tccgcacaac ctggcggtaa aacttgagca
    58081 tacgggtctg gacgcacacc ttgatttatt actttccacc cacacatttg gttatccgaa
    58141 agaggatcag cggttatggc atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac
    58201 gctgtttatt gatgacagcg aagcgattct cgatgctgcc gcgcaatttg gtattcgtta
    58261 ctgcctcggc gtgactaatc ctgattccgg gattgccgag aaacagtatc aacgccatcc
    58321 gtcactgaat gactaccgcc gcctgatccc ctcgctaatg tgaaggagac gccatgaaag
    58381 agaaacctgc tgttgaggtt cgactggata aatggctatg ggctgcccgt ttttataaaa
    58441 cccgcgcgct ggcccgtgaa atgattaaag gcggtaaggt gcattacaac gggcagcgca
    58501 gcaagccgag caaaatcgtc gagctgaatg ccacgctcac tctgcgccag ggaaatgacg
    58561 aacgcacggt gattgtaaag gcgattactg aacagcgtcg ccccgccagc gaggcagcct
    58621 tgctgtatga agagactgcg gaaagtgtag agaaacgcga aaaaatggcg atggcacgta
    58681 aacttaatgc cttaaccatg ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg
    58741 acctgttacg atttaaacac ggcgacagtg aataactgtc acctgcaaga gagatgatta
    58801 tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac
    58861 tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa ccacgattat ccgcagcccg
    58921 ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct gttaaccgct acgctgaagt
    58981 ttgatggtga tatcaccgta cagctgcagg gcgatggtcc gatgaatctg gcggttatta
    59041 acggtaacaa taaccagcaa atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa
    59101 atgccgacct gaaaacgctg gtcggcaatg gttacgtggt gatcaccatt accccgagcg
    59161 aaggcgaacg ctatcagggc gtagttggtc tggaaggtga taccctggcg gcctgcctag
    59221 aagattactt tatgcgttct gaacagctgc cgacgcgcct gtttattcgc accggcgacg
    59281 tagacggcaa accggctgca ggcggtatgt tgttgcaggt aatgcctgcg caaaataccc
    59341 agcaggacga ctttgaccac ctggcgacgc taaccgaaac catcaaaacc gaagaactgc
    59401 tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca cgaagaagag gtgacggttt
    59461 acgatccgca ggatgtggag ttcaaatgca cctgctcgcg tgaacgttgc gccgatgcgc
    59521 tgaaaacgct gcctgatgaa gaagttgata gcatcctggc ggaagatggc gaaattgaca
    59581 tgcattgtga ttactgcggt aaccactatc tgttcaatgc gatggatatt gctgaaatcc
    59641 gcaacaacgc gtctccggca gatccgcaag ttcattaatg agtatgtccg gcagagaggg
    59701 ctctgccgga ttttacgact taatggcctg atgtgctgcg cttaccagcg cctatgaaat
    59761 aaatcgcaaa ttattttggt cagatacagc gtcgtatccg ataaaagcgt tgtcacttac
    59821 ttaagtttat ttccagcgtt tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg
    59881 tccccagttt tccatccttc gcagctgtga gtaatttttc tttcgttatt tcgttcggaa
    59941 gtaaactcat atcaagcgcg tcccattctt ttctgtctga gtcaaagcca tagacaatga
    60001 ctctgtcatc attaaaagca aacaggatcc gctccgcctg accatcacta ttcagatctt
    60061 gctcgaccag tacgcaggcg tctttttcaa tacaggtcat cacgttatag cgatcctgga
    60121 ttaaggccga ccagaatgtc gcatccggtt taacagaacc aggggcaatt aacacatttt
    60181 cggctaatac tttttccgat acctgttcct gaagatgttg ctctccatct aacgccatca
    60241 gcagatcccg cgcgcgtttc gggtctttca taaacccggc atcgcttttc agcgactcaa
    60301 gcgccgcacg tccatagcga ccgctctgtt cgagcatgta gatagttacc tggtctggcg
    60361 tgtttttgcc gctctgataa cgcgccatat gactgttcac gctaatacgc atactgtcca
    60421 gcaccggcga attaagaagc accagtatca ccaacaccaa taacgaaacc gcaaggttca
    60481 ctttgccctg aagaacaagg ggattttgcc cattacgcca gacgatactg acaaaatacc
    60541 ccagcgacca caccagtaac accagctccg ccagcgcgcc ctgcaagcgg tcgacagtcc
    60601 agccgtactg agcgacccgc agccataacc cccaggtggc gacgaacaca tacagcggag
    60661 caaccagcaa agcggtttta atcaggcaac gcaacggccc cgtccaggga agtgacgctt
    60721 tttgcggatc gcggacaata gccattaaga tcaattgcaa aaaggccagc gttaacagca
    60781 acccggcggc ggagatgtgg cgagaaatcg cgctcaggcc cgtaaacggc agggtgatga
    60841 taaacatcag ggttagcaat gatactaacg gcagcaaccc cgtggcgatt agcgtgaaca
    60901 acttttgaat agagtcgatt aaacgtgact gtgtacgcgc gaggatcacc gccagtgcgg
    60961 taaccagacc aaatgtaaga tacataaacc agtcggttgc aaaaaagagc gtattgaaaa
    61021 acgtgatgcc aacaagttta aacaactcac cccacagtaa aagcaccagc cacgtcaggc
    61081 cattggagag aaaaatcacc agtaatataa gtacgttatg ccatactgac tggtaaaagt
    61141 agctatagcg gctactgcca ttgcggatac gaaggctttg ttgtatccac ggtagcaaca
    61201 acattgccat caacagcaga tagcagccaa aatcccaaat cgccttttca gctctccagg
    61261 ggttcatgcc atcagtttgc cacttcagcc aaccgctcat ccctgtcgtg gcaataaaca
    61321 ccagcgccag ccatccccaa aggcgcttct gtttaaaaga gatcacggag aagagtaaaa
    61381 cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat ccaactgtga ttttttccgg
    61441 caagccaggc gataagcagg tagcaaacca gcccctgaag taatccggta gcaatcatcc
    61501 cccagcgtgt tgccggtgaa agttcaacgt tatccatgtt ggttatccag aatcaaaagg
    61561 tgggttaatt atcgcatccg ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa
    61621 agttagcgtg gtgaatcgat actttaccgg ttgaatttgc atcaatttca ttcaggaatg
    61681 cgattccact cacaatattc ccgccatata aaccaagatt taaccttttg agaacatttt
    61741 ccacacctaa aatgctattt ctgcgataat agcaaccgtt tcgtgacagg aatcacggag
    61801 ttttttgtca aatatgaatt tctccagata cgtaaatcta tgagccttgt cgcggttaac
    61861 acccccaaaa agactttact attcaggcaa tacatattgg ctaaggagca gtgaaatgcg
    61921 cgttaacaat ggtttgaccc cgcaagaact cgaggcttat ggtatcagtg acgtacatga
    61981 tatcgtttac aacccaagct acgacctgct gtatcaggaa gagctcgatc cgagcctgac
    62041 aggttatgag cgcggggtgt taactaatct gggtgccgtt gccgtcgata ccgggatctt
    62101 caccggtcgt tcaccaaaag ataagtatat cgtccgtgac gataccactc gcgatacttt
    62161 ctggtgggca gacaaaggca aaggtaagaa cgacaacaaa cctctctctc cggaaacctg
    62221 gcagcatctg aaaggcctgg tgaccaggca gctttccggc aaacgtctgt tcgttgtcga
    62281 cgctttctgt ggtgcgaacc cggatactcg tctttccgtc cgtttcatca ccgaagtggc
    62341 ctggcaggcg cattttgtca aaaacatgtt tattcgcccg agcgatgaag agctggcagg
    62401 tttcaaacca gactttatcg ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga
    62461 acagggtctg aactccgaaa acttcgtggc gtttaacctg accgagcgca tgcagctgat
    62521 tggcggcacc tggtacggcg gcgaaatgaa gaaagggatg ttctcgatga tgaactacct
    62581 gctgccgctg aaaggtatcg cttctatgca ctgctccgcc aacgttggcg agaaaggcga
    62641 tgttgcggtg ttcttcggcc tttccggcac cggtaaaacc accctttcca ccgacccgaa
    62701 acgccgcctg attggcgatg acgaacacgg ctgggacgat gacggcgtgt ttaatttcga
    62761 aggcggctgc tacgcaaaaa ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa
    62821 cgctatccgt cgtgatgcgc tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga
    62881 ctttgatgat ggttcaaaaa ccgagaacac ccgcgtttct tatccaatct atcacatcga
    62941 taacatcgtt aagccggttt ccaaagcggg ccacgcgact aaggttatct tcctgactgc
    63001 tgatgctttc ggcgtgttgc cgccggtttc tcgcctgact gccgatcaaa cccagtatca
    63061 cttcctctct ggcttcaccg ccaaactggc cggtactgag cgtggcatca ccgaaccgac
    63121 gccaaccttc tccgcttgct tcggcgcggc gttcctgtcg ctgcacccga ctcagtacgc
    63181 agaagtgctg gtgaaacgta tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg
    63241 ctggaacggc actggcaaac gtatctcgat taaagatacc cgcgccatta tcgacgccat
    63301 cctcaacggt tcactggata atgcagaaac cttcactctg ccgatgttta acctggcgat
    63361 cccaaccgaa ctgccgggcg tagacacgaa gattctcgat ccgcgtaaca cctacgcttc
    63421 tccggaacag tggcaggaaa aagccgaaac cctggcgaaa ctgtttatcg acaacttcga
    63481 taaatacacc gacacccctg cgggtgccgc gctggtagcg gctggtccga aactgtaatg
    63541 atttgaagct ggagaatatc tatccagtat cttatagaaa gcaaaacggg aggcaccttc
    63601 gcctcccgtt tatttaccct tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca
    63661 gccaggcgcg aatggaaagc ccgccccgct cgctggtgcc aagctccagc atcccgttat
    63721 ggttatccac gatacgctgc acaattgcca gccctaatcc cgtgccgcta atggtgcgcg
    63781 cactgtcgcc gcggacaaac ggctggaaca ggtgcttacg ttgttccggc gcaattcccg
    63841 gaccgtcatc ttccacctgg aaccaggcgc gattcggctc cgttccgctg ctgactttga
    63901 tccagccatt gccataacgg gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg
    63961 acagcgggtg cattttcact tcaatgctgc cggggtaaag cgcggtttca atttcccgct
    64021 catagccact ttcggcagca atcacctcac cgagcactgc attaagatcc gccatttcca
    64081 tcggcatctc ctgcccggtg cgcaggtagt cgataaactg ctcaatgatg gcgttgcact
    64141 cttcgatatc tttattgatc gattctgcca gatagccatc ctgctcgctc atcatctcag
    64201 tcgccaggcg aatacgcgtc agcggcgtgc gcaagtcgtg acttaccccc gccatcagca
    64261 gcgtgcggtc atccgccagt tgcttaacac cagccgccat atggttaaag gcacgggtaa
    64321 cggaacgcac ctccgaagcg ccatattcac gcagcggcgg cggaataatc cctttaccaa
    64381 cctgcaaggc tgcgtgttcg agatcgacca acggtcggtt ctggatacga ataaacagcc
    64441 acgccccgcc tatcgccaat agcataatcg ccagcgtata gcggaacagc ggagagaaat
    64501 cgccctgatg aatttcggtc agcggcacgc gtacccagat attgggcgac agccaggttt
    64561 tcagccagac gacaggcgaa cttttgttga cctcaacgcg cacttccgtc gggccgccca
    64621 gttgctgtgc catctgatgg cttaagaatt catagtgttg cgcccaacgc agacctgcct
    64681 cttcggcagc ctcgttggag tagagagaga tccccagctc acggtagatc tcccgacgga
    64741 aagcgggagg cacaaccaac tgcgtgccgt cctccagttg cagtttatcg gtcatcaaca
    64801 tacgcacttc gtacgcgagg actttattaa actgctggag gctcggcaaa atcgcgaagt
    64861 tcagcaccac cagataagtc gtcaccaggc tggcgaacag caaggtgacg atgagcaata
    64921 acgtacgggc aaatgaactt cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc
    64981 cggtacaaag acgtagccca ggccccaaac ggtctgaatg taacgcggat gcgctggatc
    65041 ttcttctacc atgcggcgca gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga
    65101 atattcacga ccacgggcaa ggttcatcag cttatcgcgg gagagcggct cacgcggatg
    65161 gctgaccagt gccttcagta ccgcaaactc accgctggtg agcggcatcg gctcatcttc
    65221 gcggaacatt tcgcgcgtac cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc
    65281 ttcctgtgac ggtgcgcctg gcagttcgtt cgcctgacga cgcagcaccg cacgaatacg
    65341 ggccagcagt tcacgcgggt taaacggttt tggaatgtag tcgtcagcgc caatctccag
    65401 gcctacgata cggtccactt cttccccttt cgccgtcacc ataatgatcg gcatcgggtt
    65461 gctctgacta cgaagacgtc ggcaaatcga caagccatct tcaccaggta acattaaatc
    65521 cagtaccata agatggaaag attcacgagt cagcaggcga tccatctgtt ctgcattagc
    65581 gacgcttcga acctggaagc cttgttcggt gagataacgt tccagcagcg cacgcaggcg
    65641 catgtcgtca tcgaccacca gaatcttgta gttctcttgc attgtttgta ctcccaaagg
    65701 ttcgcaacaa tttgtaagcg tgtattctta aaaaagctca cgttcgtcac cagctaaatc
    65761 tggtatgaat ttcagcctaa attgttacaa agcatattaa acagcagctt aagtatacaa
    65821 tttattcggc gaaacattat tgattctgtt gatatgatca cgttataccc aatgtgcgca
    65881 ttatcaaaca gacaaaggga atcaacgaga tgaaaacgcc cctggttacc cgggaagggt
    65941 atgaaaaact caaacaagag cttaattatc tctggcgtga agaacgcccg gaggtcacaa
    66001 aaaaggtgac ctgggccgca agtctgggcg accgcagcga aaatgctgac tatcagtata
    66061 ataaaaagcg tctgcgtgaa atcgaccgtc gcgtgcgcta tctcactaaa tgcctggaaa
    66121 atctcaaaat tgtcgattac tcccctcagc aggaaggcaa agtctttttt ggcgcgtggg
    66181 tggagattga aaacgacgat ggcgtgactc accgtttccg tattgtcggc tacgatgaaa
    66241 tttttggccg taaagattac atctctatcg attccccgat ggcccgcgca ttgctgaaaa
    66301 aagaagtcgg cgatctggcg gtggtgaata cccctgccgg ggaagcgaac tggtatgtta
    66361 atgctatcga gtacgtgaaa ccgtaaggaa gagtcttaac ctcctgccga tggctggcat
    66421 ttttgccagc cagtccgtat aactatcccc tgatttttga tccgaaaaga tgaactcaaa
    66481 ccatgatgaa tgattcgttc tgccgcatta ttgcgggtga aattcaggcg cgcccggaac
    66541 aggttgacgc tgccgttcgc ctgcttgatg aagggaatac cgtgccgttt atcgcacgtt
    66601 atcgtaagga aatcaccggc ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc
    66661 tgagctatct gcgcgagctg gaagagagac gtcaggcgat cctcaaatcc atttccgagc
    66721 aaggcaaact caccgatgat ctggcgaagg ccatcaacgc caccctaagc aaaaccgaac
    66781 tcgaagacct ctacctgccc tacaaaccta aacgccgcac ccgcgggcaa atcgccattg
    66841 aagcagggct tgagccgttg gctgacctgc tgtggagcga tccgtcacac acgccagaag
    66901 tcgccgctgc acaatatgtt gatgccgata aaggcgtggc agataccaaa gccgcgctgg
    66961 atggcgcgcg ctatatcctg atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag
    67021 tgcgtgatta tctgtggaag aacgcgcatt tggtttctac ggtggtgagc ggtaaagaag
    67081 aggaaggggc gaaattccgc gactatttcg atcatcacga accgttgtcc acggtgcctt
    67141 ctcaccgcgc gctggcgatg ttccgtgggc gtaacgaagg cgtactccag ctttcgctga
    67201 atgccgatcc gcagttcgat gagccgccca aagagagcta ttgcgagcaa atcatcatgg
    67261 atcaccttgg cctgcgcctg aacaatgccc cggcggatag ctggcgcaaa agcgtagtga
    67321 gctggacctg gcgcatcaag gtgctgatgc atctggaaac cgaactgatg ggtaccgtgc
    67381 gcgaacgcgc ggaagatgaa gcaatcaacg tctttgcccg taacctgcac gatctgctga
    67441 tggcggcccc tgccggactg cgtgcaacga tgggcctcga tccgggtctg cgtaccgggg
    67501 taaaagtggc ggtggtcgat gccactggca aactggtggc gaccgatacc atttacccgc
    67561 acaccggaca agccgcaaaa gcagcgatga ccgttgctgc cttgtgtgaa aaacataacg
    67621 ttgaactggt ggcgatcggc aacggtacag cttcccgcga aactgaacgt ttctatctcg
    67681 acgtgcagaa gcagtacccg aaagtgaccg cacagaaagt gatcgtcagc gaagctggcg
    67741 cgtcggttta ctcagcttcc gagctggcag cgcaggagtt cccggatctc gacgtttcgc
    67801 tgcgtggtgc ggtatctatc gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa
    67861 tcgatccgaa atctatcggc gtaggtcagt atcagcatga cgtcagccag acgcaactgg
    67921 cccgcaaact ggatgcagta gtagaagact gcgtaaacgg cgttggcgtc gatctcaaca
    67981 ccgcttctgt tccgctatta acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca
    68041 tcgtggcctg gcgcgatgag aacggccagt tccagaaccg tcagcaactg ctgaaagtca
    68101 gccgtctggg gccgaaagcc ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg
    68161 ataacccgct ggacgcctct accgttcatc cggaagccta tccggtggtg gaacgcattc
    68221 tggcagcaac acagcaggca ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc
    68281 tgaaagcgtc tgactttact gatgaaaaat tcggtgtgcc gacagtaact gacatcatca
    68341 aagagctgga aaaaccgggt cgcgatccgc gtccggaatt taaaaccgct cagtttgccg
    68401 atggcgtcga gacaatgaac gacctgcaac cgggtatgat cctcgaaggc gcagtgacca
    68461 acgtcaccaa ctttggcgcg tttgtcgata ttggcgtgca tcaggacggc ctggttcaca
    68521 tctcttcatt gtcgaacaag tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca
    68581 ttgtgaaggt gaaagtgctg gaagtggatc ttcagcgtaa acgtatcgcc ctgactatgc
    68641 gtctggacga gcagcctggc gaaaccaact ctcgtcgcgg cggcggtaat gaacgcccgc
    68701 aaaacaaccg cccggcagcc aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg
    68761 cgatgatgga tgcgctggcg gcagcgatgg gtaaaaaacg ttaaacgccc ggactggccc
    68821 ctaaacctgc gcgataaggc gttcacgccg catctggcaa cgaacgcttt gcctgacata
    68881 aaagtgccgg agaatatctc cggcactttt attccacagc caaactcaca atatactccg
    68941 ttaatattta tcacttcatt aacaactgaa accttaatta aacattagcc agtccgggta
    69001 actcactatt cgaattatat tttcgctgcg atataacctt gagccacatc aacattgagt
    69061 cagattatta ttcaaaccaa cattcgtaca cattttaagt attgctgata gaaaccattc
    69121 tcattatcat tgtgttgttg attatttaat ctctcctttg ttggcaaatc atctggtctc
    69181 atgtcgcggt caaacgcccc atgaggtagt tatccagtta atgagaaaca agtaggcacc
    69241 tatgcaatac actccagata ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc
    69301 ggcatatcgc caaaaactgc tttctctagg catgttacct ggctcctctt ttgatgtggt
    69361 gcgcgtcgct ccactcggcg accccattca tatcgaaacc cgtcgtgtga gcctggtatt
    69421 acgcaaaaaa gatctggcct tattagaagt ggaagcggtt tcctgttaat acggtgataa
    69481 caacaatgaa aaaattaacc attggcttaa ttggtaatcc aaattctggc aagacaacct
    69541 tatttaacca gctcactggc gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg
    69601 aacgtaaaga agggcaattc tccaccaccg atcatcaggt cacgctggtg gacctgcccg
    69661 gcacctattc tctgaccacc atctcatcgc agacctcgct cgatgagcaa atcgcctgtc
    69721 actacatttt gagtggcgac gccgacctgc tgattaacgt ggtggatgcg tctaaccttg
    69781 agcgtaacct gtacctgacg ctgcaactgc tggaactcgg cattccctgc atcgtggcgc
    69841 tgaacatgct cgacattgcc gagaagcaaa atattcgtat tgaaattgat gctctatcgg
    69901 cacgtcttgg ctgcccggtg atcccgctgg tttcaacccg tggtcgcggt attgaagcgc
    69961 tcaagctggc gattgatcgc tataaagcta acgagaatgt ggaactggtg cattacgcac
    70021 agccgctact caacgaagca gattcactgg caaaagtgat gccttccgac atcccgctga
    70081 aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg
    70141 ccggtgaagc gtcgcagcat ctggatgcca ccctcgcccg tctgcgtaat gaaatggatg
    70201 atccggcgct gcacattgcc gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg
    70261 taagcaacac cctgacggca gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc
    70321 tcaaccgttt cctcggtctg ccgattttcc tctttgtgat gtacctgatg ttcctgctgg
    70381 ctatcaacat cggcggggcg ttacagccgc tgtttgacgt cggctccgtg gcgctatttg
    70441 tgcatggtat tcaatggatt ggctacacgc tccacttccc ggactggctg actatcttcc
    70501 tcgcccaggg cctgggtggc ggtattaaca ccgtgctgcc actggtgccg cagatcggca
    70561 tgatgtacct gttcctctcc ttccttgagg actccggcta tatggcgcgt gcagcgtttg
    70621 tgatggaccg cctgatgcag gcgctgggct tgccggggaa atcctttgtg ccgctgatcg
    70681 tcggtttcgg ttgtaacgta ccgtcggtaa tgggcgcacg tacgcttgat gcaccgcgtg
    70741 aacgtctgat gaccatcatg atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct
    70801 tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc cctggcggtt ttctcgctgt
    70861 atatgctcgg tatcgtgatg gcggtgctga ctggcctgat gctcaagtac accatcatgc
    70921 gcggtgaagc aacgccgttt gtcatggagc tgccggtcta ccatgtacca cacgttaaaa
    70981 gcctgattat ccagacctgg cagcgtctga agggcttcgt tctgcgtgct ggtaaagtga
    71041 ttatcatcgt cagcattttc ctgagcgctt tcaacagctt ctcgttgagc gggaaaatcg
    71101 tcgataacat caacgactcg gcgctggctt ccgtcagccg ggtgataacc cctgtcttca
    71161 agccgattgg cgtacatgaa gataactggc aggcaacggt tggcctgttt acgggcgcaa
    71221 tggcaaaaga agtggtggtg ggtacgctca acaccctcta caccgcagaa aatattcagg
    71281 acgaagagtt caatccggcg gaattcaacc tcggtgaaga gctgttcagt gcggtagatg
    71341 aaacctggca gagcctgaaa gacaccttca gccttagcgt actgatgaac cccattgaag
    71401 ctagcaaagg cgacggtgaa atgggtaccg gggcgatggg cgtgatggat cagaaattcg
    71461 gtagcgcagc tgccgcatac agctacctga ttttcgtcct gctgtatgta ccatgtatct
    71521 cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt
    71581 gggggctgaa tatcgcttac tcactggcaa cattgttcta tcaggtcgcc agctacagtc
    71641 agcacccaac ttacagcctg gtgtgcattc tggcggtaat cctgtttaac atcgtagtta
    71701 tcggtctgct gcgccgcgcg cgtagccggg tggatatcga actgctggca acccgcaagt
    71761 cggtaagcag ttgctgcgca gccagcacca ccggtgattg ccattaatgg cttcacttat
    71821 tcaggtacgt gatttgctgg cgttacggtg ccgtatggaa gcgacccaga taagccagac
    71881 attgaacact ccgcagccaa tgattaacgc catgctgcaa cagctggaaa gcatgggcaa
    71941 agccgtgcgg attcaggaag aacctgacgg ctgcctctct ggcagttgta aaagctgccc
    72001 ggaaggcaaa gcctgtctgc gcgagtggtg ggcgctgcgt taaccatact ccatcgcctg
    72061 ttttgaaaag cccggtatgc gtttgcatcc gggctttttt gcgtgcggct ttccataaaa
    72121 atgcaattct tgcagcgcaa cgtaagttcc tctgaaagcg tctcgcaggg atgaaaactc
    72181 gctaatgcac agatgtggag tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga
    72241 acgccatgag taaaaagcag agttccaccc cacatgatgc gctgttcaaa ctctttttac
    72301 gccaaccgga gacggcacgc gattttcttg cgtttcattt accggcaccc attcacgcgc
    72361 tctgtgatat gaaaaccctc aagctggagt cgagcagctt tattgatgac gatctgcgtg
    72421 aaagctattc cgatgtgctg tggtcggtga aaacggaaca aggaccagga tacatctatt
    72481 gtctgattga acatcaaagc acctcaaaca aactgatcgc atttcgcatg atgcgttacg
    72541 ctattgccgc aatgcaaaat caccttgatg ctggatacaa aacgttgccg atggtggtgc
    72601 cattgttgtt ttaccacggt attgaaagcc cctatcccta ttcgctgtgt tggctggatt
    72661 gtttcgccga tcccaaactg gcaaggcagc tttatgcctc cgcatttccg ctgattgatg
    72721 tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg tatggcgctg ctggagttaa
    72781 ttcaaaaaca tattcgtcaa cgcgatctga tggggctggt agagcaaatg gcctgcttat
    72841 taagtagtgg atacgctaat gacagacaaa tcaaagggct gtttaattac atactgcaaa
    72901 ctggcgacgc tgtacgtttt aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac
    72961 acaaggagag tttaatgact attgcggaaa gattgcggca ggagggggaa caatccaaag
    73021 ccctgcatat agccaaaata atgcttgaat ccggagtccc tcttgcagac atcatgcgat
    73081 ttaccgggct gtcagaagaa gagttggctg cggcgagtca gtagatactc taccctgctt
    73141 aagtgctact ttgtgctatc ttgcatgtaa cacaaagtag cacttaggga actgatatgc
    73201 agacagtgaa attacggcaa caaggaggag ccatgatcgt tactatccca cgagatcttg
    73261 ccatcgatct tggctggtca ccgggcacag agcttaccgt ggagaaaaaa ggcgatagcg
    73321 ttaatttgcg cgccacagag cacaaaccgc gtgggcgtct taccgtcgcg cagttgttga
    73381 gccaaatcga tgaaagcgaa attacggagc taaatcagtc cacagaagga tgggctgaag
    73441 ggaaaaaagg taatgaagca tggtaaaggc acggacgcca catcgtggtg agatctggta
    73501 ttttaaccct gatccggttg ccgggcatga acttcagggg ccacattatt gcattgtggt
    73561 aacggacaaa aaactcaaca atgttttaaa agttgctatg tgctgcccga tttcaacagg
    73621 ggcaaatgca gcacgttcca caggggtgac ggtgaacgtc ctcccctgtg atacgcaaac
    73681 cggtaacctg catggcgttg tactttgtca ccagctaaaa gccgtcgatc ttattgcccg
    73741 tggcgctaaa tttcataccg ttgccgatga aaaattgatt agtgaagtta tcagtaaact
    73801 ggtgaattta atcgacccac aataattagc tctcacttac caccaacacc ctctgcttca
    73861 acgccaccag caggtgacaa aactcgaccg gatgcgaaat aaacggcgca tgggccgctt
    73921 tggcgaagat atatgattcg ctgtgaggcc aaagtttatc cagcatcggc accactttgc
    73981 gcggcaccag accgtcgaga tagccataca atcgcaaaaa cggcatggac acgttttgca
    74041 gcggctgacg gagatcgacc gttttcagga tttccagccc gccattaagc acgtcaacct
    74101 ccggcatcgg taacgccaga acggttttct tcaacgcccg cgcatcctgg cgcgccgttt
    74161 cagtccccat ggtttgtaac gccaggaacc gctccactgt acgctgaaaa tcatcactga
    74221 gttgctgctg aaatcccgcc agcacgtccg gttttatccc cggccactcg tcacgagcac
    74281 taaaataagg tgacgacgcc acggtgacca gcgcctgaac acgctcggga tgggttaacg
    74341 caatctggct tgccaccagc ccgcccagac tccagcctaa ccaaatggct ttatcaggtg
    74401 cctgttgcag cacggcttcg gccatatcag caagtgacag cgcaccaaat ccccggctac
    74461 gcccgaagcc gggcaggtca acaaggtgca gcgtaaaatg cgagctaagt tcctcgtcaa
    74521 tgcaacgcca cacttcggca ttcagtcccc atccgtgcag cagcacaaga tgaacattcc
    74581 cctgaccttt ggtctgccac cagatgttat tcatccgcta ttgttctctt ttgacttaca
    74641 aggatgaaca tatgctaaca gtaccgggat tatgctggct atgccgaatg ccactggcgt
    74701 taggtcattg ggggatttgt tcggtctgtt cacgcgccac ccgcacagat aaaacgttat
    74761 gcccacaatg tggattaccc gccacacact cccatcttcc ctgcggtcgc tgcctgcaaa
    74821 aaccgccgcc ctggcaaaga ctggtcacgg ttgccgacta tgcgccgccg ttaagtccgc
    74881 ttatccacca gcttaaattt tcccggcgca gtgaaatcgc cagcgccctg tcacgtctgt
    74941 tactattgga agtcttacac gctcgtcgca ccaccgggtt gcaattgccg gatcgcatca
    75001 tcagcgttcc gttatggcag cggcgtcact ggcgtcgggg atttaatcag agcgatttgc
    75061 tgtgtcagcc gttatcacgc tggttgcact gccaatggga tagcgaagcc gtcacacgta
    75121 cacgggccac tgcgacccag cattttctca gtgcccggct gcgcaagcgc aacctgaaaa
    75181 atgcctttcg tcttgaattg cccgtgcaag gtcgccatat ggtgattgtg gatgatgtcg
    75241 ttaccaccgg aagtaccgtc gcagagattg cgcagttgct tttacgcaat ggtgcggcga
    75301 ctgtccaggt ctggtgcctt tgtcgaacct tgtagagcct cgatgatggg cgtattataa
    75361 ccaactaaaa tagtcaacta ttaggccatt actatgatcc gtatttccga tgctgcacaa
    75421 gcgcactttg ccaaactgct ggcaaatcag gaagaaggga cacaaatccg cgtatttgtg
    75481 attaaccctg gcacgcctaa cgctgaatgt ggtgtttctt attgtccgcc ggatgccgtg
    75541 gaagccaccg acactgccct gaaatttgac ctgctgaccg cgtatgttga tgagttaagc
    75601 gcaccatacc tggaagatgc agagatcgac tttgttaccg accagttggg ttctcagtta
    75661 acgctgaaag ccccgaacgc caaaatgcgt aaagtggcag acgatgcacc gctgatggag
    75721 cgcgtggagt atatgctgca gtcgcagatc aacccacagc ttgctggtca cggtggtcgc
    75781 gtttcgctga tggaaatcac cgaagacggt tacgccattc tgcaatttgg cggcggctgt
    75841 aacggttgtt ccatggtcga tgtgacgctg aaagaaggga tcgagaagca gctgctgaac
    75901 gaattcccgg agctgaaagg tgtgcgcgat ctcaccgaac accagcgcgg cgaacactcc
    75961 tactactaag ttatcccctc ttttgtagat tgcccgatgc gacgctaaag cgtcatttcg
    76021 ggcctacgtt gaacatgtgc cagacgttgg cacagcattg ctttggcaaa tccctccctt
    76081 cgtttccccg cgataatatg accaacctct cataatttaa atttaccccg ctctggtgat
    76141 tctcaaacgc cagatgttac ccgtatcatt cacatgggta ccaaacatac tcctgacatc
    76201 tgactacaat aattagtttt agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg
    76261 attgggataa ttagagtttg tcgtcagaaa attgacgtta cccataacaa atgaaaggcc
    76321 aggtaaatca tgccattagt cattgttgct atcggtgtaa tcttgttgtt gctcctgatg
    76381 atccgcttca aaatgaacgg cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta
    76441 atgcaaggaa tgccgctgga taaagttatt ggctccatca aagccggtgt cggcgggacg
    76501 ctcggtagcc ttgccctgat catgggtttt ggcgcaatgc tggcaaactg cggtggcgca
    76561 caacgtatcg ccaccacgct gattgccaaa tttggtaaaa agcacatcca gtgggcggtg
    76621 gtgttaaccg gttttaccgt tggttttgcc ctgttctatg aagtgggctt cgtgctgatg
    76681 ctgccgctgg tgtttaccat cgcggcttcc gcgaatattc cattgctgta tgttggtgta
    76741 ccaatggcgg ctgcactgtc tgtgacccac ggcttcctgc caccgcatcc gggcccgact
    76801 gcgattgcca ccattttcaa tgccgatatg ggtaaaaccc tgctgtacgg tactattctg
    76861 gcaatcccga ccgtgattct cgccggtccg gtttacgctc gcgtgttgaa aggtatcgat
    76921 aagccaatcc cggaaggtct ctacagcgcg aaaaccttca gcgaagaaga gatgccgagc
    76981 tttggcgtca gcgtctggac ctctctggtg ccggttgtgc tgatggcgat gcgtgcgatt
    77041 gccgaaatga tcctgccgaa aggtcacgct ttcctgccgg tagcggagtt cctcggtgac
    77101 ccggtaatgg caacgctgat tgccgtgctg attgcgatgt tcacctttgg tctgaaccgt
    77161 ggtcgttcaa tggatcagat taacgacacg ctggtttctt ccatcaaaat cattgcgatg
    77221 atgctgttga tcatcggtgg tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg
    77281 gacaaataca ttgcttccat gatgcacgaa accaacattt ctccgctgct gatggcctgg
    77341 tcgattgctg ccgtactgcg tatcgcgctg ggttccgcaa ccgttgcggc aatcactgcg
    77401 ggtggtatcg cggcaccgct gattgcaacg acgggtgtta gcccagagct gatggttatt
    77461 gcggtgggtt ccggtagtgt gattttctct catgtgaacg atccgggctt ctggctgttc
    77521 aaagagtact ttaacctgac tatcggcgag accatcaaat cctggtcgat gctggaaacc
    77581 attatctcgg tgtgcggtct ggtaggctgt ctgctgctga atatggtgat ttgataacac
    77641 tgactgccgg atgcatcgca tccgacatca accgcactct acttcttctt cgctgcagct
    77701 ctgcgccgtc tgtccaaatc cttcagcaac ttgttcacgc catcatcggc aaacatcgac
    77761 tcaagcgttg cggaaagctt gcgtcgccag tttttatact ggtaactggt gccaggaata
    77821 ttcaccggtt cggccatatc cagccagtct tccggctgta gtcctaacag agcactgtta
    77881 ctgtcggcaa tgtagcgctg caaaccacgg ttcagcgtcg gcgtcatcga catcaacgat
    77941 gccttatgcc cggcacgttt tggcagacaa ccatatttat gcagtgcatc cagcagccct
    78001 tgcttcgcca gttcgcgatc ctgatacaga ccgcgcagta ccacttcatc cggatacagc
    78061 cccagggttt tgcccagcgt tagatccccg ctctcccagt aaccacgcag cgttggcagg
    78121 tcatgtgtcg ccgcaaccgc catcgactgc tccggatacg ctttcggtgc acggaacgtc
    78181 ttctcgtggt cgttttcgaa atagagcact ttgtaagagt acacaccgct gctgcgcagc
    78241 ttaccgacaa tctctaccgg tacggtaccg agatcttcac caatcaccat acagcgatga
    78301 cgtttacttt cgagtgccag aatcgagagc agatcatcca ccggatagtg aacatacgcg
    78361 ccctgatctg ccgtctcgcc atacggtatc caccacaaac gcagcatcga catcacatgg
    78421 tcaattcgta atgcgccgca gttttgcata ttggcacgca acagctcgat aaacggttca
    78481 taggcacgcg cggtgatgat atgcgggtcc attggcggta atccccagtt ctgccccaac
    78541 gggccgagga tatccggcgg cgcgccaacc gatgctttca ggcaatatag ttcacggtca
    78601 caccaggttt ccgccccacc ttccgctacg ccaaccgcca gatcacgata caagccaatc
    78661 ggcatttcat agccctggct tatctcccag caggcggcaa actggctgta agccagccac
    78721 tgcaaccaga gataaaaatc gacgtcatca cgatgttctt cgcagaactg acgcacttct
    78781 ggtgaatcca cgttcttata catctctggc catgcaggcc agccccagcg catttcgtcc
    78841 tctttcactt gctgggcatg tagcgcatca aaggctgcct gccagaacag gctgtcgccc
    78901 tgctctgcaa caaactggcg aaacgcggcc atctgctcat catcacgttg cgcgaaacct
    78961 ttccacgcca ttcgtaatgc tgtcattttt agggcggtaa ccgtggagta atcgacccag
    79021 tcggcatcgc gcgcctgttg cagcgtctgt tgcgtggtcg gcaactgcca ccaggcctga
    79081 gcctcttcgc taagatggaa atcttcaacg gcgttaacgt cgatataaat cacattcagc
    79141 caacggcgag aagacgggct gtatgggctg gcgctctccg gatttgccgg atagagcgca
    79201 tgaatcgggt tcaggccaat gaacgacccg ccacgttttg ccacatccac cagcatcgct
    79261 ttgagatcgc caaaatcccc aataccccag tttttttccg atcgcagcgt ataaagctga
    79321 acgcaggcac cccacagctt ttgtttattc agcaaagcct gcggttcgta acaacgtttc
    79381 ggggcgacaa tcacccggca atgcgcgcgc tggtcgtcct gggtgagtgt tagcgtgtga
    79441 taaccttccg gcagcttcgt cggtagattg aacgctttgc cccccgttac atggcctttg
    79501 tactgcgttc cttcttcggt ggtcagctgc cagctatatt cgccgctgcc ctccaccacc
    79561 atcggcattt ttttgccgct ggtataaacc atgacattcg ggactggcgt taccgccact
    79621 ttcgtggcgg tacgttgatg catcgcgtca agcaaacgcc gtttggtttc ggcgctaatc
    79681 gactgcggtt taccgtgggc attgatgtaa ttggggctaa tccccgccgc cagcgcggca
    79741 ttatccagac gtttgctttc catcgagctt ccttagcgtt ttgcctgcca gatacgagcc
    79801 tgataatcgc gaatagagcg atccgagcta aacataccgc agcgggcggt attgaggatc
    79861 gccgcgcgag tccaggcctc ctggtcgcgg tacagcacat ccacctgctt ttgtgcctca
    79921 acataggctg cgaaatccgc catcaccaga tacggatcgc cgccctgttt gccgatactg
    79981 tgcagcatct ggtcgaaggc atgcttatcg ccgtcgctgt atttaccgct ttccagctct
    80041 ttcaatactg cgtccagcac cttatctttc ttccgccatt tcaccgggtc gtagcctttg
    80101 gccagaatag ccttcacttg ttccacggta tgaccaaaaa taaagatatt ttcttcaccg
    80161 actttctcgg cgatttcaac gttcgcacca tccagcgtac cgacagtaag cgcaccattg
    80221 agcgccagtt tcatattgcc ggtaccggaa gcttctttac ctgcagtcga aatttgttcg
    80281 gagatatccg ccgccgggat cagtttttcc gccgccgaaa cgcaataatc cggcaggaac
    80341 accaccttca acttatcgcc aaccagtgga tcgttgttga tcacgtcagc cactttgttg
    80401 atcgcaaaga taatattctt cgccaggtag tagcccggtg ccgctttcgc gccgaagagg
    80461 aagacgcgcg gtacgcgatc agcctgcagg ttttcacgaa tttctttgta caacgccaga
    80521 atatgcagca gattcaggtg ctggcgtttg tactcatgca gacgtttgat ctgaatatcg
    80581 aaaatcgcct gtggattgat ctcaataccg gtacgaactt tcacaaactc cgccagacgg
    80641 actttattcg cctgcttgat ctcgcgatat tgctgacgga atttcgcatc atcagcgaat
    80701 ttttccagat tgatcagctg atcgagatcg ttagcccact ctttttgcag tgatttatcc
    80761 aacagagccg ccagtgcagg gttgcactgt ttgatccagc gacgtggggt aataccgttg
    80821 gtgacgttat ggaatttgtt cggccatagc tggtgatatt ccgggaacag atctttcacc
    80881 accagatccg agtgcagcgc cgcaacaccg ttcaccgcga aaccgccaac cacacacagg
    80941 ttcgccatat gcacttgttt atcgtgcacc accgccagtt tggcccacac tttttcatcg
    81001 cccggccagg ttttctccac cagcgtttta aagcgagtat taatttcgtt aataatctgc
    81061 atatggcgcg gcagtaagcc tttcaccagt ttcacatccc agcgttccag cgcttctggc
    81121 atcagggtat ggttggtgta agcgaaagtt ttgctggtga tggcccaggc gtcatcccag
    81181 ctcatctggt gctcatcggt cagcacgcgc agcagttctg gaatcgcgat agttggatgg
    81241 gtatcgttca gctgaataac ttcgtaatcc gccagttcgt gcagtttacg ccccgccaga
    81301 tgatggcgac gcaaaatatc cgctaccgaa caggcacact ggaagtattg ctgcatcagg
    81361 cgcagctttt taccggcagt atggttgtcg tttggataga gaactttggt cagtttttcc
    81421 gcattgatgc cttgctgttc ggcgcgcagg aaatcaccgt cgttaaattt agttagatca
    81481 aacggatgcg catgcgtcgc ctgccacaga cgcagcggct gcgccacgcc attacgatag
    81541 ccgacaacgg ggagatccca cgcttgaccg gtaatggtaa actccggctc ccagcgtccg
    81601 tctttcatca ctttaccgcc aatccctacc tgcacatcca gtgcttcgtt gtggcggaac
    81661 cacgggtagt tactgcgatg ccagtcatcc ggcgcttcaa cctgtttgcc atcgacaaaa
    81721 gactggcgga acaaaccata ttgatagttc agaccgtaac ccgtcgcaga ctgaccgaca
    81781 gttgccattg agtcgaggaa gcacgccgcc agacgtccca gaccaccgtt acccagcgcc
    81841 gggtcgatct cttcttccag caggtcagtc aggttgatgt cataagcctt caacgaatcc
    81901 tgtacatcct gataccagcc gagattcaac aggttgttgc ccgtcagacg accaatcaaa
    81961 aactccattg agatgtagtt aacatgtcgc tgattcgcca ccggtttggc gaatggctga
    82021 gcacgcagca tttcggccag tgcttcactc actgccagcc accactggcg aggagtcatt
    82081 tcagccgcag aatttaagcc ataacgctgc cactgacgtg aaagcgcttc ctgaaattgc
    82141 ttatcgttaa aaataggttg tgacatagga gttccacttt tcttagattt tcaacacaac
    82201 gttatcgcta gtttgccagg ctcgatgttg accttcctca tcctgcgggg gattaggcag
    82261 ggaggagttg cggggatgag caaggaaatg tgatctcgac cacttaaagc tagtgcaaac
    82321 cacaggatta gcagcaaatc aatgccatac agcgcagaaa atctgtatct aagtgcaaaa
    82381 aatggccgtt gcgtattttc aaaaagcgga aggtaactct ataaattaag taaaggagtg
    82441 aaacagtttc acaagtaaaa tatccagtgt gctccatctc attcttaata gatttattaa
    82501 gatcatcttt ttagatggca ctttcatcag gaatgaagaa gaaacccttg cttaaatgaa
    82561 tctgat
//
LOCUS       DADLZM010000004        76412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLZM010000004 DADLZM010000000
VERSION     DADLZM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.004"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..545
                     /locus_tag="JNY73_000269"
     CDS             <1..545
                     /locus_tag="JNY73_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HAY8046404.1"
                     /translation="FPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKEN
                     SLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVI
                     EQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASY
                     TQLEIKHSHPKNRASGKRVS"
     gene            1088..1580
                     /locus_tag="JNY73_000270"
                     /pseudo
     CDS             1088..1580
                     /locus_tag="JNY73_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1683..2186
                     /locus_tag="JNY73_000271"
     CDS             1683..2186
                     /locus_tag="JNY73_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY8046405.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="JNY73_000272"
     CDS             complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="JNY73_000272"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY8046406.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="JNY73_000273"
     CDS             complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="JNY73_000273"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY8046407.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="JNY73_000274"
     CDS             complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="JNY73_000274"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY8046408.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="JNY73_000275"
     CDS             complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="JNY73_000275"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY8046409.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="JNY73_000276"
     CDS             complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="JNY73_000276"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY8046410.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="JNY73_000277"
     CDS             complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="JNY73_000277"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY8046411.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="JNY73_000278"
     CDS             complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="JNY73_000278"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY8046412.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="JNY73_000279"
     CDS             complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="JNY73_000279"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY8046413.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="JNY73_000280"
     CDS             complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="JNY73_000280"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY8046414.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="JNY73_000281"
     CDS             complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="JNY73_000281"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY8046415.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="JNY73_000282"
     CDS             complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="JNY73_000282"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY8046416.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16156..16818)
                     /locus_tag="JNY73_000283"
     CDS             complement(16156..16818)
                     /locus_tag="JNY73_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY8046417.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="JNY73_000284"
     CDS             complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="JNY73_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY8046418.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="JNY73_000285"
     CDS             complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="JNY73_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY8046419.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19776..20993
                     /locus_tag="JNY73_000286"
     CDS             19776..20993
                     /locus_tag="JNY73_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY8046420.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21077..21676
                     /gene="yfbR"
                     /locus_tag="JNY73_000287"
     CDS             21077..21676
                     /gene="yfbR"
                     /locus_tag="JNY73_000287"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY8046421.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21735..23567)
                     /locus_tag="JNY73_000288"
     CDS             complement(21735..23567)
                     /locus_tag="JNY73_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY8046422.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="JNY73_000289"
     CDS             complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="JNY73_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY8046423.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24315..24809)
                     /locus_tag="JNY73_000290"
     CDS             complement(24315..24809)
                     /locus_tag="JNY73_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY8046424.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24892..25347)
                     /locus_tag="JNY73_000291"
     CDS             complement(24892..25347)
                     /locus_tag="JNY73_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY8046425.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25685..26887
                     /gene="ackA"
                     /locus_tag="JNY73_000292"
     CDS             25685..26887
                     /gene="ackA"
                     /locus_tag="JNY73_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY8046426.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26962..29106
                     /gene="pta"
                     /locus_tag="JNY73_000293"
     CDS             26962..29106
                     /gene="pta"
                     /locus_tag="JNY73_000293"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY8046427.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29296..30816
                     /gene="yfcC"
                     /locus_tag="JNY73_000294"
     CDS             29296..30816
                     /gene="yfcC"
                     /locus_tag="JNY73_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY8046428.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="JNY73_000295"
     CDS             complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="JNY73_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY8046429.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="JNY73_000296"
     CDS             complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="JNY73_000296"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY8046430.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="JNY73_000297"
     CDS             complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="JNY73_000297"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY8046431.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32836..33483
                     /gene="yfcG"
                     /locus_tag="JNY73_000298"
     CDS             32836..33483
                     /gene="yfcG"
                     /locus_tag="JNY73_000298"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY8046432.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33540..33902
                     /gene="folX"
                     /locus_tag="JNY73_000299"
     CDS             33540..33902
                     /gene="folX"
                     /locus_tag="JNY73_000299"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY8046433.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIVREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33923..34816
                     /locus_tag="JNY73_000300"
     CDS             33923..34816
                     /locus_tag="JNY73_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY8046434.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34864..35754)
                     /locus_tag="JNY73_000301"
     CDS             complement(34864..35754)
                     /locus_tag="JNY73_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY8046435.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="JNY73_000302"
     CDS             complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="JNY73_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY8046436.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36732..37448)
                     /locus_tag="JNY73_000303"
     CDS             complement(36732..37448)
                     /locus_tag="JNY73_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8046437.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37445..38131)
                     /locus_tag="JNY73_000304"
     CDS             complement(37445..38131)
                     /locus_tag="JNY73_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8046438.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="JNY73_000305"
     CDS             complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="JNY73_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY8046439.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39224..40006)
                     /gene="argT"
                     /locus_tag="JNY73_000306"
     CDS             complement(39224..40006)
                     /gene="argT"
                     /locus_tag="JNY73_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY8046440.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40272..40841)
                     /locus_tag="JNY73_000307"
     CDS             complement(40272..40841)
                     /locus_tag="JNY73_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY8046441.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40936..42453)
                     /gene="purF"
                     /locus_tag="JNY73_000308"
     CDS             complement(40936..42453)
                     /gene="purF"
                     /locus_tag="JNY73_000308"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY8046442.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEEENLEMHNEG"
     gene            complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="JNY73_000309"
     CDS             complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="JNY73_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY8046443.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="JNY73_000310"
     CDS             complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="JNY73_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY8046444.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43889..45157)
                     /gene="folC"
                     /locus_tag="JNY73_000311"
     CDS             complement(43889..45157)
                     /gene="folC"
                     /locus_tag="JNY73_000311"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY8046445.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45227..46141)
                     /gene="accD"
                     /locus_tag="JNY73_000312"
     CDS             complement(45227..46141)
                     /gene="accD"
                     /locus_tag="JNY73_000312"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY8046446.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46297..46956)
                     /locus_tag="JNY73_000313"
     CDS             complement(46297..46956)
                     /locus_tag="JNY73_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8046447.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47039..47851)
                     /gene="truA"
                     /locus_tag="JNY73_000314"
     CDS             complement(47039..47851)
                     /gene="truA"
                     /locus_tag="JNY73_000314"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY8046448.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47851..48864)
                     /locus_tag="JNY73_000315"
     CDS             complement(47851..48864)
                     /locus_tag="JNY73_000315"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY8046449.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="JNY73_000316"
     CDS             complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="JNY73_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY8046450.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50165..51160
                     /locus_tag="JNY73_000317"
     CDS             50165..51160
                     /locus_tag="JNY73_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY8046451.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMPTRPALWILLVAIILMLVWLVR"
     gene            complement(51157..52335)
                     /locus_tag="JNY73_000318"
     CDS             complement(51157..52335)
                     /locus_tag="JNY73_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HAY8046452.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="JNY73_000319"
     CDS             complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="JNY73_000319"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY8046453.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDITGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53989..55995
                     /gene="mnmC"
                     /locus_tag="JNY73_000320"
     CDS             53989..55995
                     /gene="mnmC"
                     /locus_tag="JNY73_000320"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY8046454.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(56116..56394)
                     /locus_tag="JNY73_000321"
     CDS             complement(56116..56394)
                     /locus_tag="JNY73_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY8046455.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56428..56976)
                     /locus_tag="JNY73_000322"
     CDS             complement(56428..56976)
                     /locus_tag="JNY73_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY8046456.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56976..57785)
                     /locus_tag="JNY73_000323"
     CDS             complement(56976..57785)
                     /locus_tag="JNY73_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY8046457.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="JNY73_000324"
     CDS             complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="JNY73_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY8046458.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSPDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="JNY73_000325"
     CDS             complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="JNY73_000325"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY8046459.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="JNY73_000326"
     CDS             complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="JNY73_000326"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY8046460.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60831..61382
                     /gene="smrB"
                     /locus_tag="JNY73_000327"
     CDS             60831..61382
                     /gene="smrB"
                     /locus_tag="JNY73_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY8046461.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61486..62376)
                     /locus_tag="JNY73_000328"
     CDS             complement(61486..62376)
                     /locus_tag="JNY73_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY8046462.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(62363..62887)
                     /locus_tag="JNY73_000329"
     CDS             complement(62363..62887)
                     /locus_tag="JNY73_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8046463.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62884..63354)
                     /locus_tag="JNY73_000330"
     CDS             complement(62884..63354)
                     /locus_tag="JNY73_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8046464.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDIAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(63351..63899)
                     /locus_tag="JNY73_000331"
     CDS             complement(63351..63899)
                     /locus_tag="JNY73_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8046465.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63904..64626)
                     /locus_tag="JNY73_000332"
                     /pseudo
     CDS             complement(63904..64626)
                     /locus_tag="JNY73_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64646..67287)
                     /gene="yfcU"
                     /locus_tag="JNY73_000333"
                     /pseudo
     CDS             complement(64646..67287)
                     /gene="yfcU"
                     /locus_tag="JNY73_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(67369..67932)
                     /locus_tag="JNY73_000334"
     CDS             complement(67369..67932)
                     /locus_tag="JNY73_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8046466.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68616..69101)
                     /gene="sixA"
                     /locus_tag="JNY73_000335"
     CDS             complement(68616..69101)
                     /gene="sixA"
                     /locus_tag="JNY73_000335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY8046467.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(69304..71448)
                     /gene="fadJ"
                     /locus_tag="JNY73_000336"
     CDS             complement(69304..71448)
                     /gene="fadJ"
                     /locus_tag="JNY73_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY8046468.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(71448..72758)
                     /gene="fadI"
                     /locus_tag="JNY73_000337"
     CDS             complement(71448..72758)
                     /gene="fadI"
                     /locus_tag="JNY73_000337"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY8046469.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72938..73222)
                     /locus_tag="JNY73_000338"
     CDS             complement(72938..73222)
                     /locus_tag="JNY73_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY8046470.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73594..74934
                     /gene="fadL"
                     /locus_tag="JNY73_000339"
     CDS             73594..74934
                     /gene="fadL"
                     /locus_tag="JNY73_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY8046471.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75300..76351
                     /locus_tag="JNY73_000340"
                     /pseudo
     CDS             75300..76351
                     /locus_tag="JNY73_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        19317 a        20452 c        18740 g        17903 t
ORIGIN      
        1 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
       61 aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc
      121 atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggag
      181 aaatgtcgaa taataatata aaagttcatt taaatgaacc agagataata tttttacatg
      241 ccgatcttca gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg
      301 aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact
      361 atgctgacag atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc
      421 gactggtaaa cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt
      481 atacacaatt agaaataaaa cactcacacc ccaaaaatag agcatccggc aaaagagtga
      541 gttaacatca ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa
      601 tttgctaaaa agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg
      661 gtatttccag cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg
      721 ctgacttttc ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa
      781 cataattata tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa
      841 aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata
      901 aataatgatg aaataacgcc ttttattgat aatttacata tgaaaaaatt tttccttttg
      961 ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt
     1021 atcaccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac
     1081 acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta
     1141 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
     1201 gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt
     1261 atcccgatcg gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc
     1321 gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc
     1381 cagggtttat tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc
     1441 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
     1501 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct
     1561 tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
     1621 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
     1681 aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg
     1741 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
     1801 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
     1861 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg
     1921 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
     1981 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
     2041 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
     2101 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
     2161 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
     2221 gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca
     2281 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
     2341 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
     2401 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
     2461 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
     2521 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
     2581 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
     2641 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
     2701 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
     2761 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
     2821 agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag
     2881 gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
     2941 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
     3001 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
     3061 ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc
     3121 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
     3181 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
     3241 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
     3301 aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg
     3361 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
     3421 ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
     3481 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
     3541 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
     3601 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
     3661 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
     3721 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
     3781 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
     3841 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
     3901 ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc
     3961 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
     4021 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
     4081 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
     4141 cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag
     4201 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt
     4261 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
     4321 gtctgagcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg
     4381 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg
     4441 taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg
     4501 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
     4561 atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc
     4621 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
     4681 atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc
     4741 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
     4801 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
     4861 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
     4921 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
     4981 agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
     5041 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
     5101 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
     5161 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
     5221 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
     5281 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
     5341 atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca
     5401 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
     5461 ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa
     5521 gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
     5581 ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc
     5641 gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag
     5701 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
     5761 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
     5821 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
     5881 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
     5941 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
     6001 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
     6061 gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca
     6121 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
     6181 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
     6241 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
     6301 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
     6361 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
     6421 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
     6481 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
     6541 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
     6601 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
     6661 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
     6721 acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta
     6781 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
     6841 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
     6901 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
     6961 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
     7021 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg
     7081 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac
     7141 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
     7201 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
     7261 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
     7321 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
     7381 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
     7441 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
     7501 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
     7561 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
     7621 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
     7681 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
     7741 cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg
     7801 ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga
     7861 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
     7921 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc
     7981 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
     8041 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
     8101 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
     8161 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
     8221 gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt
     8281 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
     8341 gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg
     8401 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca
     8461 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
     8521 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
     8581 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
     8641 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
     8701 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
     8761 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
     8821 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
     8881 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
     8941 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
     9001 tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata
     9061 aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc
     9121 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
     9181 tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt
     9241 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
     9301 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc
     9361 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
     9421 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
     9481 ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt
     9541 tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg
     9601 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
     9661 tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca
     9721 agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtgttgc
     9781 catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg
     9841 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
     9901 attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct
     9961 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac
    10021 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    10081 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
    10141 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    10201 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    10261 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg
    10321 ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca
    10381 cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga
    10441 cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt
    10501 cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg
    10561 cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
    10621 gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca
    10681 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    10741 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
    10801 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
    10861 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    10921 cgattttgtt ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg
    10981 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    11041 ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    11101 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca
    11161 tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct
    11221 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    11281 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    11341 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    11401 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    11461 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    11521 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    11581 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    11641 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    11701 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    11761 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    11821 ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt
    11881 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    11941 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    12001 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    12061 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    12121 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    12181 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    12241 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    12301 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    12361 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    12421 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    12481 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    12541 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    12601 agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga
    12661 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    12721 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    12781 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    12841 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    12901 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    12961 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    13021 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    13081 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    13141 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    13201 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    13261 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    13321 atagcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    13381 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    13441 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    13501 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    13561 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    13621 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    13681 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    13741 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    13801 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    13861 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    13921 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    13981 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    14041 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    14101 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    14161 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    14221 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    14281 aacaaaatcg atactgccca gataaacaat caggtcagac accaagctgc cgcggatcgc
    14341 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    14401 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    14461 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    14521 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    14581 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    14641 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    14701 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    14761 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    14821 ataggcggca acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc
    14881 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc
    14941 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    15001 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    15061 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    15121 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    15181 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    15241 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    15301 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    15361 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    15421 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    15481 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    15541 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    15601 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    15661 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    15721 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    15781 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    15841 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    15901 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    15961 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    16021 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    16081 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    16141 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    16201 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    16261 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    16321 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    16381 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc
    16441 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    16501 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctggcgcg gcgaagcacg
    16561 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    16621 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    16681 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    16741 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    16801 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    16861 cgtttccggg ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    16921 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    16981 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    17041 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    17101 ggagccgacc gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    17161 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    17221 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    17281 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    17341 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    17401 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    17461 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    17521 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    17581 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    17641 gatctgtgac acgcttttgt tattccatag caaaaacgca gcaacaaatt tacgtatttt
    17701 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    17761 tctctggtga ttgaaaaata aacagttttg tacgttctgc actatccgag gaaggatttt
    17821 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    17881 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    17941 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    18001 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    18061 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    18121 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    18181 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    18241 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    18301 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
    18361 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    18421 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    18481 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    18541 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    18601 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    18661 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    18721 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    18781 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    18841 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    18901 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    18961 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    19021 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    19081 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    19141 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc
    19201 agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt
    19261 gcgaattatc tgatgcaaag ttatgttatg ttatgttatg ttaggcaaag taatcttcta
    19321 ttattaataa gcacatcaaa acctttttga atattaaata ataattaatt agcatcatcc
    19381 tcattcatta attccgctta acaatagttt cacaccttac ccccattgcg acacctcgga
    19441 aatcatcact ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg
    19501 aattcttaaa tgaggcattt tcacacaatt atcttacaga taaaaaaacc atacttgcaa
    19561 ttaagaatta gaacaattca ctatataaca ttacatgtaa agcatataca cctaattatt
    19621 ttgtcattat taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc
    19681 tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca
    19741 gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca
    19801 aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg
    19861 aagaagaagg taacaaggta ctgaaactga acatcggcaa cccagcccca ttcggttttg
    19921 acgcgccaga tgaaatcctc gttgacgtga tacgcaacct gcctaccgct caagggtatt
    19981 gcgattccaa agggctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg
    20041 gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg
    20101 ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct gcaccagatt
    20161 acccactctg gaccgcggcg gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg
    20221 atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta
    20281 cgcgtgggat tgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc
    20341 ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc gccgatgaaa
    20401 tttatgacaa aattctctac gacgatgctg agcatcactc aattgcgccg ctggcacctg
    20461 acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc
    20521 aggggtggat ggtgttgaac gggccgaaaa aacacgccaa aggctacatc gaaggtctgg
    20581 aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg
    20641 cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggcggtcgt ctttatgagc
    20701 agcgtaaccg cgcgtgggaa ctgatcaacg atattccggg cgtttcctgc gtgaaacctc
    20761 gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt catgacgatc
    20821 agaaaatggt gctggatttc ctgttgcagg aaaaagttct gttggtgcaa gggacggcat
    20881 tcaactggcc gtggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg
    20941 agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat
    21001 ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg atgatgtcat
    21061 catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact
    21121 cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt
    21181 gcaggtagcg atggtcgccc atgcgctggc agctatcaaa aatcggaaat ttggcggtaa
    21241 tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcctcag aagtgctcac
    21301 cggcgatctc cctactccgg tgaaatactt caatgcgcaa atcgctcagg agtacaaggc
    21361 tattgaaaaa atcgctcagc agaaactggt cgatatggtt ccggaagaac tgcgggatat
    21421 ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca
    21481 ggcagatgca ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg gaaataatga
    21541 attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca gccaggagat
    21601 ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca
    21661 ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca ggcaataagc gccggatgcg
    21721 acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc
    21781 acgataatgg tgaacggtac ccccaacttc acaaagtcac tgaagctgta atttcccgga
    21841 cctaaaacca gggtgttaac aggtgaagac accggtgtca taaaggcggc ggatgctgcc
    21901 atcgccacga ccatcgcaaa tggataaggc gacactccca tcgttttggc agcagccagc
    21961 gcaatcggag ccatcaacac cgccgtcgcg gtattagaga taaacagccc aataaccgcc
    22021 gacaagacaa acaaacaccc cagcatcata tgtggcccat aaccgccgcc aatatccatc
    22081 agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc aaacggcatc
    22141 atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct ttcagcatct
    22201 atacagcgga atttccccat cagcaggcag gcgatgatag cggcaacagg attaggaatt
    22261 tcatttgtca gcattaacgc caccattagc accagacaga aaatggcatg gggtgcctgg
    22321 ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac gaagtcgcgg
    22381 ccctgtttgg ccagcatacc gatcagtttc cagttaccca caaccaggat gatatcgccc
    22441 agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg cttgagcccc
    22501 accacattca gtccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt
    22561 tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa
    22621 tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg
    22681 acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca
    22741 ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagcg
    22801 ccataacgct cacgtaattt gagatcatcc agccgttgac ctatcattgg cgatccgggg
    22861 cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag
    22921 gttcgacgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag catgaaacgc
    22981 atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta
    23041 aagaaactga agccgtgata gccttcacgc agcaattcac tgttgactac caggttcggc
    23101 ggcgtcgcca ccagcgtcat catgccgcta atcagcccgg caaaacttaa cggcatcatc
    23161 agacgtgacg gcgaagtttg catacgcatg gcaacgctta acaccacggg aataaagata
    23221 gcgacaacgc tggttgaact cataaacgcg ccaagccccg cgacggtcag catcaacaaa
    23281 accaacattt tgatttcact gttgcccgcc actttgacca gccatgttcc cattacggtg
    23341 gcaacaccgg tacggaccaa accatcgcca ataataaaca aggcggcaat caggacaacg
    23401 ttaggatcag aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac
    23461 gcgacaataa caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg
    23521 atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt
    23581 taattgaggg ggatgacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc
    23641 atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt
    23701 gctccagact gtggaggacc aaatcgacct cattcaggcg cggggtatct gccggagcgt
    23761 taaccgcaat gacatggcaa cccgccgcta ggccagaaag cacgccagcg ggagcatctt
    23821 ccaccaccac acactcctgc ggcgcaagcc ccagcagctg cgcacctaac agatacgcat
    23881 caggttctgg ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa
    23941 gccccgctat tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc
    24001 acggaatacc tgctttattc aaatgattga gtaaggcgat tgccccagga agcgcggtaa
    24061 taccttcggt ttccgtggcc tcgatttgct ccaaacgcgt aaactcggcg gcaatatcag
    24121 cctcggattt gcccgccata aaatggcgca aagaggtgat cgcctgttta ccgtgaatga
    24181 aagccagcac ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt
    24241 ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc
    24301 gcacgcgggc ctcctcaggc gttgataatt tgattaattt cgttggcgct caaatggtac
    24361 tgacgcgggc aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc
    24421 tgggcgttaa aaccatgagt cccagcgtca aaatgggtat agcgcccttc cacattaacc
    24481 ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg
    24541 acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca
    24601 tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac
    24661 tcgcgatcca attcacgcat ctgtaatccg taaccacgct caataattgt ttgcaggcga
    24721 cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa
    24781 ataagacgtt gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga
    24841 aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc
    24901 atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg
    24961 gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg
    25021 aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag
    25081 ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc
    25141 aagcagaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa
    25201 acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg
    25261 tttttccagt ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt
    25321 aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa
    25381 aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc
    25441 actatcaggt atcctttagc agcctgaagg cctaaatagt acatattcat tgagtcgtca
    25501 aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat
    25561 ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca
    25621 tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac
    25681 ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc
    25741 catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct
    25801 gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc
    25861 aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc
    25921 agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta
    25981 taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt
    26041 tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc
    26101 acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga
    26161 agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta
    26221 cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa
    26281 accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc
    26341 tatccgcaac ggtaaatgcg ttgacacctc tatgggtctg accccgctgg aaggtctggt
    26401 catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct
    26461 gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct
    26521 gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc
    26581 gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc
    26641 gctgatggat ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc
    26701 gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga
    26761 acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc
    26821 tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac
    26881 tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc
    26941 ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc
    27001 gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt
    27061 ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact
    27121 acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc
    27181 tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca
    27241 aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca
    27301 cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa
    27361 atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa
    27421 ctgacccgca acagcttcgg tggtgccaaa aacaccaaca tcaccggcgt tatcgttaac
    27481 aaactgaacg caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc
    27541 gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc
    27601 ccgctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc
    27661 gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc
    27721 gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc
    27781 ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca
    27841 gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg
    27901 cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac
    27961 accaacacct ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac
    28021 gatcacgaac gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg
    28081 atcgaatctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt
    28141 tatcagctga ctgaactggc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac
    28201 gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta
    28261 ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactaggt
    28321 gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc
    28381 gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac
    28441 gtggtgctcg gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct
    28501 gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc
    28561 agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt
    28621 gactgtgcga tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc
    28681 gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc
    28741 ggtacttctg gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag
    28801 gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct
    28861 gacgttgcga aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgttcatc
    28921 ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg
    28981 atctccatcg ggccgatgct gcagggtatg cgcaaaccgg ttaacgacct gtcccgtggc
    29041 gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag
    29101 cagtaatctc gtcatcatcc acagctttgc gctgcggata tctgaaccgg aaataatcac
    29161 tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact
    29221 gcgctgcatc aatgaattgc gccatctcac tttgcatact taccactttg ttttgtgcaa
    29281 gggaatattt gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg
    29341 cccgatacgt tggtgattat cttttttgtt gctattttaa ccagtcttgc cacctgggta
    29401 gttccggtgg ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa
    29461 acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct
    29521 gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc
    29581 ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt
    29641 atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt
    29701 atcctggcgc ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt
    29761 attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc
    29821 attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg
    29881 acttatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt
    29941 gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg
    30001 tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa
    30061 aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg
    30121 gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt cctgacagcc
    30181 gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc
    30241 agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac
    30301 ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc
    30361 cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggttggtaa tggtgaagca
    30421 ggtaatgcca gcgtgttaaa tactatcctc aacagcattg ccaatgccat tagcggtctg
    30481 gacaacgcgg tcgcggcctg gtttatgttg ctcttccagg ctgtatttaa tttcttcgtg
    30541 acgtccggtt ctggtcaggc ggcattaact atgccgttac tggcaccgct tggcgatctg
    30601 gtcggtgtta accgtcaggt taccgtgctg gcgttccagt ttggtgatgg cttcagccac
    30661 atcatttacc caacctcagc ttcgttaatg gcaacgctcg gtgtgtgccg ggtggacttc
    30721 cgtaactggc tgaaggtggg cgcgaccctg cttggactgc tgtttattat gtccagcgtc
    30781 gtagtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg
    30841 ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc
    30901 cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct
    30961 tccggcgtca gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga
    31021 gagacgcaac tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc
    31081 ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg
    31141 gattccagca gttgctcatc ggcctgaact acaccgcctg cggtcgcatc taacatgccg
    31201 ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc
    31261 acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct
    31321 tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga
    31381 cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg
    31441 tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt
    31501 acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata
    31561 ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg
    31621 gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa
    31681 agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg
    31741 gttatcggga aatgcagcat catttgatcc acttcgctgt cgcagttgcc acgcacagcg
    31801 ataaccttat gtgccacttc attaagccgt tcagcaactt tggctggcgc gtaaccctcc
    31861 ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca
    31921 ccgctttgga caaacaactc cacaacacgt tccgtcgccg gtaacgaccc atgaatgtcc
    31981 gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg
    32041 attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga
    32101 cgctcgctgc cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg
    32161 tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa
    32221 taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact
    32281 ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg
    32341 acgctcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg
    32401 cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc
    32461 ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa
    32521 cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc
    32581 gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga
    32641 taacacataa ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat
    32701 tatgtcctcg gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca
    32761 cgtgacgaat actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa
    32821 acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt
    32881 acgctgtttc tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa
    32941 ggcggtcagt ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt
    33001 gttgatcatt ctcctgccga tggcggcgaa ccgttaagcc tctttgagtc tggtgccatt
    33061 ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc
    33121 gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat
    33181 catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt
    33241 gaaactcagc gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga
    33301 ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc
    33361 ctgcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg
    33421 cgccctgcca ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt
    33481 taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa
    33541 tggcacaacc tgccgctatt attcgtataa agaaccttcg tttgcgtacg tttatcggaa
    33601 ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc
    33661 ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa
    33721 cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc
    33781 aggatgtgct cgatatcgta cgtgaacatc actgggtgac gtatgctgaa gtggagatcg
    33841 ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt
    33901 aatcgccata tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg
    33961 tcgccatttg attccgcgtt tgctggagct gggccatcaa attacggtag tgacgcgtaa
    34021 cccgcagaaa gccagttccg ttctcggccc tcgggtgaca ctatggcaag ggcttgccga
    34081 tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga
    34141 taaacgctgg actcacaagc aaaaagagcg tctctgccaa agccactgga atatcacgca
    34201 aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca tttccggctc
    34261 ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca
    34321 taacgaattt actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag
    34381 tgacaaaacg cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat
    34441 tctcggtaaa atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg
    34501 gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga
    34561 taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt
    34621 tgcccatgcg ctcggtcatg cgctgcatcg cccggctatt ttgcgcgtcc ccacaaccgc
    34681 cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc
    34741 taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc
    34801 ggatgtcgtt cgctgatgtg gtttacagca aacatccgcc agttaactcc ctgtgttaca
    34861 ggattagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa
    34921 ctctctgtct aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt
    34981 gccctgcatt gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc
    35041 ttgtggtgcg cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt
    35101 ttgtaatacg taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag
    35161 cagcgaaaca atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat
    35221 taactccaac agcgccattt tgcggtgttg cataatctcg tcatccggca ccactgtaat
    35281 atccaccaac ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc
    35341 atcaagccag cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg
    35401 gatcaccaat ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga
    35461 ataacgcatc atgcgaaaag ccattaattc ttccggctta ctttggtgct ctatcactac
    35521 ataaatataa cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg
    35581 ccgcaggtct tcatcaataa aactgtttgg ttctagttta agcgtcgtta aatcacacag
    35641 tttgcgcagc ggcgcgggaa gatgaatatt aataaaattc cgcgcggtgt ctggatggcg
    35701 taaaaaagat ttaaataccg catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt
    35761 ccgtcacccc tcaaatttaa gatgacgcga caataaccac gattaacacc caatgcagca
    35821 ggcagttttt atctttggac agcgaattca gaagtatttt tagcggactg caacttttac
    35881 atttcagcgg aagctgactc gcaaattccc cttgaaaata ctccggcagc gttagccacc
    35941 ggagaggaaa ttatttcagc gatcccttaa ggaactgttg cagacgaggg ctttgcgggt
    36001 tgccaaataa ctgctccggc gcgccctctt cttctatttt cccctgatgg aggaaaatga
    36061 cgtgagtaga aacatggcga gcaaagccca tttcgtgagt cactaccacc atggttttcc
    36121 cctcttctgc cagttgctgc ataatacgta acacttcgcc taccagttca ggatcgagcg
    36181 ccgaggtagg ttcatcaaac agtaaaactt ctggttccat cgccagcgcc cgcgcgatag
    36241 aaacacgctg ttgctgaccg ccggaaagat gcaccggata tttcccctgc gcacgttcgt
    36301 ctatcccgac tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc
    36361 ccaacacctg aatcggcgct tccatgacgt tttccaacac cgtcatatgg ctccagagat
    36421 tgaagtgctg gaataccatc gtcaggcgtg tgcgcagtaa gcgcagttga tttttatcgg
    36481 cgactttgag ttgaccgtct ttgtcgcgca ccagattgat cgtctggcca ttgaccacga
    36541 tcgatccttc actcggtttt tcgaggaagt taatgcagcg cagaaaggta cttttccccg
    36601 atcccgacga tccgatgatg cttattacat ctccggcatt cgcttgcagt gagacccctt
    36661 tcagcacttc gtgttcgcca tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg
    36721 acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg tttttccgct
    36781 ctgcgaaaga ggctgatcag gacataagag atgattaaat agagcaccgc ggcaatgccg
    36841 aaggcggtaa aaggttgata cgtggcggcg ttaatatcgc gggctatttt cagcaaatcc
    36901 ggcacagtgg ccgtaaacgc cagcgcagta gagtgcagca tcaggatcac ttcgttgctg
    36961 tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcagcgata cattttaaag
    37021 gtcgagaagc cataggctct ggcggcttca atttccccat gcggaaccga acggattgcc
    37081 ccagcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag cacggtacag
    37141 ttcaggccac tgcggaagaa agcgttaagg aattcggttc ccttaacaat ctcaagcgtg
    37201 tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg aaaaatatag
    37261 gtaaataacc agattggaaa ctggatgtat ttattactgg agacacgacc aatcgccaga
    37321 aacagcgcca ggactccgcc tatcactacc gacaaaataa gcagccacag agtgatcgcc
    37381 acaccagtaa agcgataacc gtcggtccac agcagcggtt tccagtattc atgtaagatt
    37441 tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg aacagcagca
    37501 caccattgga aacggtggtg aaaaccaggt aaatcacgcc acagacgatg gcgaaataga
    37561 acggttccca ggtacttttg cctgccagct gcgtggcttt gaccacatct tccaggccga
    37621 gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca atgcctggca
    37681 gggcgtaacg catcatcgcc ggaaacatga tcctccgaaa cacttgcccg cgagtaaaac
    37741 cgaacgccgt cgccgcctct atatgccctt tcggcactgc cataaacgcg ccacggaacg
    37801 tttcggtaaa ataagcaccg tagataaaac cgagagtgat aataccagcg accatcggat
    37861 cgatatcaat ctgcccgacg cccatcgcct ccgtcaccat gtttagcgca atctgcaaac
    37921 catagaaaat cagcagcatc aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc
    37981 cttcgaaaat cagccccaaa agccgatttt gcgagagctt accgccagcg ccaattaaac
    38041 cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg agcgcaccct
    38101 gtaaaataac acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt
    38161 accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca ccgatggcga
    38221 ttaaccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt aagtaccgtc
    38281 agcgcgcatt tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat cttctttacg
    38341 caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg gaccaccgaa
    38401 tttgtaatct ttaccgacgg gttgtttgag gaaaccttcg ctggcagcga cctcatcctg
    38461 gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct gcccctgata
    38521 cgagacgatt tcaatgcctt ttggtgccca gtgttcatta ccgaacgtct cctgggtggt
    38581 gccctgcaat acgcctaccc gtttgccttt cagcgactcg actgtcggct gaatgtcaga
    38641 atttttcgcc accaccagac gagaatcggc agcgtacagt ttgtcggtga aggcaatttc
    38701 ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt
    38761 taaggacggg attaacgcat ccagcgggtt ttcgacaaac gtacattgcg tattgatgcg
    38821 cttgcacaac tccttcgcca gctcgatatc gaaaccaacc agttcgcctt gtgaattttt
    38881 tgattcaaat ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg gaattgcagc
    38941 aagtgccgca gttgcgctgg agaaggccag aaccagagag agcgatagca ccagtttttt
    39001 cataatgtcc tcaactgaca gtctttttat agggaatatt tacaggtttg ttacacttat
    39061 cgtgccataa aattgaccat caaggcaaaa tatcccgccc cgttagacgt attatttttc
    39121 attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa cctggcgtgc
    39181 accatgatga tcattccatc aggtacagct tcccagcgac gtatcagtca ccgtagacat
    39241 taaagtcgaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa
    39301 gcgccttatt gaaggcagcc gtcagttcag catcatcttt acgtagccct acaccggtgc
    39361 catcaccgaa gtattttttg tcttttactg atgagccagc aaaggcgaaa tctttaccag
    39421 caggttgctt gaggaatcct tcgctggcag caacttcatc ttgtaacgca gcatccagac
    39481 gtcctgcagc cagatcggaa tagaccaaat cctggttggc ataggccacc acatccacgc
    39541 ctttactacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc agcacaccaa
    39601 catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg gcagcaatca
    39661 aacgagaatc tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg
    39721 taatggaaag cgacgaaata atagcgtcga tttttttcgc tttcagtgag gggagcagcg
    39781 cgtcaaagtc actggcaacc caggtacatt tcacctgcat ccgtttgcac atctcgttac
    39841 cgagatcgat atcaaagcca acaaaatcac ctttagcatc tttcgacgag aacggcgcat
    39901 aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg
    39961 cggagagacc gactaataaa gacagagcga gaatcgactt cttcatacat aaccctcaag
    40021 tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg
    40081 tcttatgcca tcttgacgac aacagtaaca ttcaacgtta aatatgttaa taagacgttg
    40141 cattattgtc ctgaagttga agatagcagg tatagcggtt gaatcgcagc gtttcgattg
    40201 aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc tgaaacaggg
    40261 caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag ggttattgca
    40321 aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga ttgcgggcga
    40381 tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc ctgagtcatt
    40441 aaacgcagat ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc
    40501 tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct atggacaatg
    40561 ccggacaggg ttttgattga acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg
    40621 gaagagatgc tggcggaaat atcgcgcgca tcgagcgtga catcagctaa tgcctgcact
    40681 tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg gctcatcacc
    40741 agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataagcgcac gccataaatc
    40801 gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg ccctgtcaga
    40861 cttgcaggca gactttgcag tatttcggcg ggagttgcaa gtcagggtgc cagaccggca
    40921 ccctcagcga aggcatcatc cttcgttatg catttcgaga ttttcctctt cgttctgacg
    40981 ttgcactgct ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt agccctgatc
    41041 aacatctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct ggatatccgg
    41101 attttcagcg cgaacggcgt cgatcagatc gttcagatcc tggaaaatca acccgtcagc
    41161 accgatgatc tggcgaattt catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct
    41221 cggcatatca ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta
    41281 cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag tggtgccacg
    41341 gacgatggag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt
    41401 cagtttacgg cgcacggact tacgacgcag ctgctggccc ggcatgataa aggtgcggcc
    41461 aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac gagcaatttc
    41521 cagcgcgata tcacacgagg tttctgggat cgggatcacc acgtcgatat ccagatcttc
    41581 ccattcgcgg gcaattttct cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac
    41641 ggaaattttg tcgataaacg agtccgggcg ggcaaagtat acatactcaa acaggcacgg
    41701 attgctgacc ggattgtcag cacattgacg ggtaaacaac tgcccttctt cagtgatgta
    41761 aatcgcttcg cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac
    41821 gctttcggaa gcgaccatat attctgtacg gttctcgtca atatcacgtt ttcccagtac
    41881 cagcggacga atcccgtttg gatcgcggaa agcaaccata ccgtggccga taatcatcgc
    41941 cacacaggca tacgcgccgc ggattaagcg gtttgtggca gcaatggcag cgaaaatatt
    42001 gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctggcgaaga tattaagcag
    42061 aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg
    42121 caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat acggggagtt
    42181 aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt aacgcacatg
    42241 accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata catcgctcac
    42301 cagcccgttc gctttacgca aacggaagca gttattggca tctatggtga tgatgccggc
    42361 ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact ggttaaccgg
    42421 cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt aagccacatc
    42481 tcagagcact tacgctctgg gcaagaaact tgacgagctt tgcagataat caaaaaacca
    42541 tctgatgata aaactgaatt gcgggatcag ctgtgatttg ctccagtctt cacttttcga
    42601 caccccggta aaggagtcga gaaagaagag aatggcggca acaatcaaca caccgcgcaa
    42661 cgcaccgaaa cagacgccca gcacccgatc ggtgcctgac aaccccgttt tctccaccaa
    42721 ctggcctatc acgaagttca cgatagcacc aacgatcagg gtagcgataa acagtaccgc
    42781 gatggcaatc ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta
    42841 agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg ataacgcttc
    42901 acgaacaaag ccgcggatca ggctaaccag agaggaaaaa gcaatcaccg cgattatggc
    42961 gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct gttcggggcg
    43021 cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa
    43081 tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca tcgtgcaatg
    43141 cgtgtcggat gcggcgtaaa cgccttatcc gacctacggt tctaacactg cccaagcctg
    43201 ataagacgcg ccagcgtcgc atcaggcaag actgtgttaa ttcggcgtat agcccattac
    43261 cacgccactt aagccagaaa gttgcttcaa ctcacccagc gaacctttca gcttatcttt
    43321 cgaggcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg tggatggcga
    43381 cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca ctttatcggc
    43441 atttttcagc gcacccagtt gcacaacata ggctttaccc gttggcgctg ctttttcttc
    43501 cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaacctttg gtttaggcgg
    43561 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    43621 attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc
    43681 ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc
    43741 aggctcatca cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata
    43801 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    43861 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    43921 cttccatgac atgtgcgacc gtgtggaaag aaccacacac cagcacggtg tcttccgctt
    43981 tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat ttgccgttac
    44041 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    44101 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    44161 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga
    44221 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    44281 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    44341 tgacttccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    44401 caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    44461 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg
    44521 ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    44581 ggaagatgcc tgctttctcg cgaccaatac tttcgcgatc tggacccagc cagtcggtat
    44641 gatccagcgc aatactggtt actaccgcgg catcggcatc gacaatattg gttgcgtcca
    44701 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc
    44761 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    44821 caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcccggccc tgcacacgta
    44881 cgcgctcggt ataacgcacc agatgaggcg aactgtagac gcccactttg taccctgccg
    44941 ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta cccgcaacgg
    45001 taaacacaaa tggcgctggt ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct
    45061 caaggccgag ctcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    45121 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    45181 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    45241 cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    45301 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    45361 cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat ctaggcggca
    45421 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttag
    45481 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg
    45541 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agtgccgcag
    45601 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    45661 cggagaagca gatcagcggg cagttatctt cctgcgcctg ctcaacggca cgcacgaaac
    45721 gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat gccgcagcga
    45781 caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    45841 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagctcat
    45901 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    45961 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    46021 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    46081 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    46141 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    46201 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    46261 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    46321 gcgtttgtga cggattattt cgatgacgcc cggcaaaata gaaaccacaa taatcccgac
    46381 gatcagcagc ttaaggttat cctgaaccat cgggattgta ccgaagaaat agcctgcgta
    46441 ggtaaaaagc agtacccaca acagtgcgcc gatcacgtta taagcagcga aatgacggta
    46501 cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg
    46561 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt tgtcgagata
    46621 actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga acaaccgccc
    46681 gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca ctaccatcat
    46741 atggacgttg agatcgttgg tttccagtga tgccaacgct ccggcaacaa acagcaatga
    46801 atcacccggt aaaaacggca ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    46861 aatggcataa acccagacgc cgtactccgc gactagctct gccagatgca cgtcaatgtg
    46921 caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct tgtactcgtc
    46981 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattctcgtt
    47041 agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat
    47101 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    47161 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    47221 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatcaacca
    47281 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    47341 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    47401 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    47461 ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa
    47521 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    47581 aattcgcatt tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat
    47641 gcaccacttg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag aagacggtga
    47701 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt
    47761 cattctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc agcgcaattt
    47821 tataaactgg cagttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    47881 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagag
    47941 ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt
    48001 acccgaagca tcccctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc
    48061 gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt
    48121 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    48181 cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    48241 accttcgcta tccggcaaca acggcagcat attgaacgcc agttgacgac cgaagaaatc
    48301 ttcttcgtca atcggaatgc cgttgagcaa tttcgcactc tgccccgcta acgcatcgac
    48361 cgcttttttg ccctgggcgg aggctgaaat caggctggta acactgatac gcgacaaacc
    48421 gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac tgtctggtac
    48481 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcaccac
    48541 cagcggtacg tcgggttcga gagcaaacag tccgctgctg tcgatcacca ggcaacctga
    48601 gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag
    48661 ctgcgcctgc gtccagtcga attcagcggc atcctgcacg gtgattgttt taccaccaaa
    48721 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    48781 ctgacgttca gccagcgttt caagcagggc ttcgcccata gcgccagttg cgcccaggac
    48841 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    48901 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    48961 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    49021 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    49081 actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    49141 agcgtcggtt gatcgagcgg accatgcagc gtaatgcggc caaactcagg cgcgggcagc
    49201 aacgtatcca gcgcaacgtg ttgttcatgc ccaataaact tgctataggc ttcaaacact
    49261 tgcgtcgtgc cacgcgcttt accctccagg gtatagcctg cgatatgcgg cgtgccgata
    49321 tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac atccagcacc
    49381 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    49441 ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag tttttcatcc
    49501 gccagatgta gcgttttgta cggaccatct ttaaagagtg gcgtatggaa agtcagaata
    49561 tccgcgcgct ggactaactc atccagcgag cggaaatcac cctcatcccc acggtcggcg
    49621 cgaggcggat cgcaaagtaa ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga
    49681 cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga aaatccatcg
    49741 cgttcggcaa gcatcagcag ggaggagaaa acatattcca ccaccgcaat cgcattacag
    49801 ccaggtgcag cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc gacatggtcg
    49861 gtccccgcag tggcagttcc aacaaattta atgggttttc ctgccagcaa agattcattc
    49921 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc gacggggatt
    49981 ggacgcccgg gaaccgcgat cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    50041 atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg
    50101 gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata
    50161 cgtaatgata caacctattt ccggccctcc tcctgggcag ccaccaggtc agggagataa
    50221 cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag
    50281 cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt gggcgggtat
    50341 caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc
    50401 tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct
    50461 tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg tcacggatta
    50521 tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac
    50581 cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc agcgcgaggc
    50641 gaccgaccgt cctttattac cggcggagca caatgcgcta aaacagctgg tgaccaaact
    50701 tgcggcggca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg aactttccgg
    50761 ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggctaca
    50821 ggcgcgtcag acgctaagcc agcaaaatac gccgacgctg gaatcactac agatggcgct
    50881 aaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata
    50941 tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct
    51001 ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc ttaatccttt
    51061 tccaccgatg atggacacgt tgcaaaatat gccaacgcgt cccgcgctgt ggatactgtt
    51121 agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa atgacagtat
    51181 cgtgacaata atacccagca ccgcagagat cgccccggca agaaataccg aagagtaacc
    51241 aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga tatcctgaaa
    51301 cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt taaccacctc
    51361 cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa
    51421 tgcgacccat gcacctgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag
    51481 agagacaatc gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg
    51541 catcacgaca aatgcgccgc caaacgcggt aagagtaaag cccgccatcg cccatccttt
    51601 gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc
    51661 cagccctaac cctggtttcc agataagccc gacaacgctc cacagcgatg gacgttctcc
    51721 cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatac
    51781 cattgtggtg atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg
    51841 cgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatca ctttgccaga
    51901 gtgttttggc cctacgatgc ctaaccccca ggtcagagcg cctgtcagta actggctttc
    51961 accaaaccca agaatcaaac gcccgatgac caacagggca aatttgaacg gtgcggagac
    52021 aggcaaaatc gccgccagca gcaacgcgcc gccagccaga ccacaagcta acatcccctg
    52081 aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac cacgcgtcag
    52141 caccgtagcc agaaactgaa tcccgacggc aatgccgacc atggtattgc catagcccag
    52201 atcatgatga acaaacagcg ggataaccgg caacggcaac cctacggtca tgtaggtgag
    52261 aaaaaccgca aaagcgatgc ggaagagcga aaaactggca gaagatcgtg tttcggtttg
    52321 gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc
    52381 atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga
    52441 aggaaatcga caaagcggga agtttgccta gaactggtgg cgattgtcaa tgatgtgaaa
    52501 aagggaacca tcaggttccc ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc
    52561 ggatgcgacg ctggcgcgtc ttatccgacc tactccggcc tactgcgaat taatctttca
    52621 gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataacag
    52681 tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acccgcagcc tgctcgtcca
    52741 gctcttcaat gttgatgctc ggggcgataa agccgtgttc cagcatcagc agagagtaga
    52801 tagcttcctg cacacccgca gcgcccagtg agtgaccggt catggctttg gttgcagaaa
    52861 tcgccgggct cttatcgccg aacacttcac ggatagctgc cagctctttc acgtcgccaa
    52921 ccggagtcga agtaccgtga gagttcaggt aatcgattgg ggtatcaacg ccatgcatcg
    52981 ccatctgcat acaacgtact gcgccttcgc cagacggagc aaccatgtct gcaccatcag
    53041 aggttgcgcc gtagccaacg atttcagcat agatgtaagc accacgcgcc agcgcgtgtt
    53101 ccagctcttc aaccactacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag
    53161 cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac agcgcaccca
    53221 tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc gccgccgcca gcaaacacga
    53281 tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg
    53341 cacacgcgga gctgatggag tagttaacgc catgaatttt aaacggcgtg gcgagacagg
    53401 cagaaacgcc ggatgccatc gctttagtga ccacatacgg gccaaccgct ttcaggccgc
    53461 gcgggccgcg cattgcgtca gcgccgaaca cctggaaacg cggggaaccg ccgccggaac
    53521 ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggagagagg cccgcatcag
    53581 cgattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa
    53641 ctttgcggtc aatgaggcca gtgatatcca gttttatgtt gccccagacg tggctacgca
    53701 tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg
    53761 ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg
    53821 cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc
    53881 gtacacttgt acgccgaaca agtccgatca gccatttaat agagaaattt gcgcagcctt
    53941 acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac
    54001 tccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt
    54061 gacgatgtct atttttccaa cgataacggg ctggaagaga cgcgttatgt ttttctggga
    54121 ggtaaccaat tagaggtacg ctttcctgag catccacatc ctctgtttgt ggtagcagag
    54181 agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttga tcagtttcgc
    54241 gaagcgcatc cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc
    54301 ctcacccgtg cggatttagc cttagcgcat caacactggc cggaactggc tccgtgggca
    54361 gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat
    54421 gaaggccgcg tgacgctgga tttatggttt ggcgatatta acgaactgac cagccaactg
    54481 gacgattcgc taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagctaaa
    54541 aacccggata tgtggacgca aaatctgttt aacgccatgg caaggttggc gcgtccaggc
    54601 ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggaagccgga
    54661 ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg tggggtgatg
    54721 gaacagacat taccactccc ctgctccacg ccgtggttta atcgtacggg cagcagcaaa
    54781 cgggaagtag cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct ggcgttatta
    54841 cggcgcggct ggcaggtagc gctttattgc gcggatgaag cccccgcgct gggtgcttcc
    54901 ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc
    54961 tttttctcta atgcgtttac ttttgctcgt cggttttacg accaattacc cgttaaattt
    55021 gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc
    55081 gcacagatgt tgtcaatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg
    55141 gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt
    55201 tggctgtgcc cagcagaact gacccgtaat gtgctggaac tggcgcaaca gcagggtttg
    55261 cagatttatt atcaatatca gttacagaat ttatcccgta aggatgactg ttggttgttg
    55321 aattttgcag gagatcagca agcaacacac agcgtagtgg tactggcgaa cgggcatcaa
    55381 atcagccgtt tcagccaaac gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc
    55441 catattccga caacgccgga actggcagag ctgaagcagg tgctgtgcta tgacggttat
    55501 ctcacgccac aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc
    55561 agcgaagata cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat
    55621 tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc
    55681 ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag gcaatgttcc cgattatgag
    55741 gcaacactcg tggaatatgc gtcgttggcg gagcaaaaag atgaggcggt aagcgcgccg
    55801 gtttttgacg atctctttat gtttgcggct ttaggttctc gcggtttgtg ttctgccccg
    55861 ctgtgtgccg agattctggc ggcacagatg agcgacgaac cgattccgat ggatgccagt
    55921 acgctggcgg cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg
    55981 gttaaggcgc ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc
    56041 aacctacgtg tgacgtgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc
    56101 tccagacgcc gcaacttaca gctgtgacgc ctgctgaaac aggttttccc acatatcggt
    56161 caccagcgcc tggtcacgcg gcgacaattc accggcacca atggcttttt ccagactctg
    56221 gctaacggtc gtatgcaccg cctgagcgga gtggtcatca ccactttcca gttctgcgat
    56281 ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg
    56341 gtcaaccatg ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt
    56401 tcctcattgt aaaacgtggc gctggcttca gttgagcgct tccggccacg ggaactgttc
    56461 cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccgggcg
    56521 ttccggaatg ccatttgtca gataatccat cacctgcgca tgtacccgcc gttgaaacac
    56581 cacacgatcc ggttcgaaat ccccttccag attgtcgcaa ctgacgttaa acggatatcc
    56641 agccgccacg cagaataacc agtcgaacgc ctgcggcttc acttcaacat cttcaaactg
    56701 gctttgcgtc tgggcatcgc gtccgtccgg gcaataccag tagccgaaat caaccagctc
    56761 acggcgcgct tttccggcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa
    56821 gccatgagca aagacgattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg
    56881 ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat
    56941 ttcaatcaac tgctcgtagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg
    57001 atctcctgtc cgtggctatc gtaaagtaat ttggcactca tcaccgccga gacgataaca
    57061 atcatcgggc ggatcagctt ttgtcctttg ctcaacacca accgtgaacc catgcgcgcg
    57121 ccgaggaact gccccaccag catgacaaag cccgtcgccc aaatcacttt gccgccgaga
    57181 ataaacagca gcagaccgcc aatgtttgac gttgcgttga gtaatttggc gtgagccgtg
    57241 gctttggcga ggttgaatcc gcacagcata acgaaggcca gcgcgtaaaa cgatccggca
    57301 gccgggccaa agaatccgtc ataaaaaccg acgcagccac cggcaattaa cgcgaagggt
    57361 aaaccgtaca tccggcgctg gcgatcttct tcacccagct ttggcatcag caagaaatag
    57421 aggccgatac aaatcaccag aatgggcaaa atctgccgta agacatcagc ctgaacgtac
    57481 tgtaccaaca gtgcgccgct cattgagccg acaaaggtca tggcaatatt gagtttctga
    57541 tcgcttaaac taaccacttt gcggcgaata aagtagatag tggcggaaat agagccgccg
    57601 caggcttgca gtttattggt tgccagcgcg ttagcgggag acattcccgc agccatcaat
    57661 gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg
    57721 gcgacaaaaa agagaactcc cagcaacagc ggagaaacca taaacaggct attaaacgtt
    57781 tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg
    57841 tcttcttctc aggctttgtt gttcccggtt ttggaggttc aaaccagctt tgcagttctg
    57901 ccccgcaacc atcgcctgat ggcggtaaag gttgatcttc acactccaga ctatcggcag
    57961 ggcaacgtaa tcgtacatgc atatgcgcgc gatgctggaa ccagggtcgc actttgcgca
    58021 accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa
    58081 aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat caagctgaaa atttctggct
    58141 tccacagcgt ggagacaacg tgtttaccgt cgggggaaac taagtccagt gcttgcgggc
    58201 gcaagagctg cgcggaggtc cagcgagttt tcggcagttg cagaaagata tcgacatcca
    58261 gtccggtctg gtggctggca tgaccgccgt tgaaacgccc accagcgggc atccccatat
    58321 cgccaatcag caccgtaccc atgcccagat tgctcacctg gctactcaga cgctggataa
    58381 acatcaccag atccgggtga ccgaaatagc gacgctgatc ggtacgcatg acctgataat
    58441 gttcggactg tatcggcaac gtgtcagcgc cgacaataca gccattagaa aaactgccta
    58501 tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc
    58561 tagcgctact ggcaagcaga gccagcagcg caatcgcggt tttgttcatt ttttaccagc
    58621 gtggaatatc agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg tgatccatta
    58681 aaacgatcgc cagcatcgct tcagcgatcg gcactgcacg gatcccgaca cagggatcgt
    58741 gacggccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatagtg cgccccggca
    58801 cggtaatgct ggaggtcggt ttcagcgcca tatgagcaat gatttgctgc ccgctgctga
    58861 taccgccgaa aatgccgccc gcatggttgc tctggaaacc ctctttggtg atttcgtcgc
    58921 ggttctggct gccgcgcagc gccactacgt caaaaccgtc gccaatttcc acgcctttca
    58981 ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc caggcggtca aagaccggct
    59041 cgccaagtcc ggcggggaca ccactggcaa caacggtgac tttcgcgccg atggagtcgc
    59101 cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcg gggtccgggc
    59161 agaagaacgg attttgttca accagcgacc agtctttgat ttccagcgga atgtcgccca
    59221 tctgggtcag gcagccgcgg atttcaatac caaatttctc ggcgagatat tttttggcaa
    59281 tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga agaacgtcca ccgccgcgat
    59341 aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc atggcctgga cggaagacgt
    59401 ctttaattgc gctgtaatcc tgagaacgct ggtcggtatt ttcgatcaac aagccaatgc
    59461 tggtgccagt agtaacgcct tcaaaaacac cggagagtat tttgacctga tccggctcgc
    59521 ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg atcaaggtca tgttgcaggt
    59581 ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg agcgccagcc
    59641 cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt ccagccatca
    59701 cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc gaaatgttct
    59761 cgtgcggcaa gaagctgctc tttagtgagc ataaacacac catcgccgcc gttatcaaac
    59821 tccagccagg tgaacggaac atcgggatat tgttccataa gatgtaccat gctgttgccc
    59881 acttcacaaa tcaacacgcc atcatcagca aggtaatctg ccgcgttacc gagaatgcga
    59941 cgcgtcagtt tcaggccgtc ggtgccagat gccaggccca gttccggctc gtgacggtat
    60001 tcgtttggca ggtcggacat atcttccgcg tcgacatacg gcgggttagt gacaatcaga
    60061 tcgtattgca ctttcggcaa atcacggaac agatcggaac gaatcggaat gacgttgtgg
    60121 atcagaccgt gttcttcgat gttctgttcg gcaaccgcca gcgcgtcggg agagatatct
    60181 accgcgtcga cttccgcttc cgggaaggca taagcacagg caatggcgat gcagccgcta
    60241 ccggtacaca tatctaaaat atgctgcggt tgcttgctga tgagtccggc aaatttattg
    60301 ttgatcagtt caccaatcgg cgagcgcggc accagcacgc gttcatcgac gtaaaattca
    60361 tgaccgcaga accaggcttt gttggtcagg taagccaccg gaatgcgctc gttgacgcgg
    60421 cggatcacgc gttcaacaat acggtgtttt tcgctggagg tcagacgcgc ggtgcgcata
    60481 tcttccggga tatccagcgg caggtagagc gatggtaaca ccagctgtac ggcttcatcc
    60541 cacgggttat cggtgccgtg accgtaccag atatttgccg cgctgaagcg gcttaccgac
    60601 cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg cttcatcaac gaaaatttta
    60661 tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg ccatgaagat
    60721 gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt
    60781 gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacagataa atgaaaaaga
    60841 aaacaacact tagcgaggag gaccaggctc tgtttcgcca gttgatggcg gggactcgca
    60901 agattaagca ggacacgatt gtccaccgac cgcagcgtaa aaaaatcagc gaagtgccgg
    60961 tgaaacgctt gatccaggag caggctgatg ccagccatta tttctccgat gagtttcagc
    61021 cgttattaaa taccgaaggt ccggtgaaat atgttcgccc ggatgtcagc cattttgagg
    61081 cgaagaaact gcgccgcggc gattattcac cggaattgtt tttggattta cacggtctga
    61141 cgcagctgca ggccaagcag gaactgggcg cgttgattgc cgcctgtcgc cgcgaacatg
    61201 tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat tttgaagcaa caaacaccgc
    61261 tgtggctggc acaacatccg catgtgatgg catttcacca ggcaccgaaa gagtacggcg
    61321 gtgatgcggc tttgttggtg ttgattgaag tggaagagtg gttgccgccg gagttgccct
    61381 gaatgctggc agcaaatgcc ggagcagcgt aaacgcctta tccggcctac aaatatgtga
    61441 taattcaata aattgcaatt cacttcgtag gcctgataag acgcatcaga gcaatactac
    61501 gaacttcagt tcattcaata catactgaaa acgacacgtc gaatcaatcg gttgcccact
    61561 actggtattc acattcacca caatatcgtg tagtacttta tccgttaccg gatgacgagt
    61621 tggcgtaacc gttaccgttg atttatcaca accgtaagag gtaaaggtta acaattctga
    61681 taccgcgcgg cagtatgctc ctctgccatc attatcagaa aaattactgc catcacagag
    61741 tctgccgcct tccatcaggt aggtcgaaag cgtggtggtc actgttgacc ccgtggactg
    61801 aattcgtaac gccttattat ggctgacata attttcactc caggagatat cgtatgaagc
    61861 gaccggtatc gttgggcata ttgtgttatg cactgtaggg gccgtatagc ttccaaatcc
    61921 gggtaaatct gtataggaat aaaaggatgc gatccaaggg caaccataat catcagatgc
    61981 aacggtaatt ttaaacgtca tatatgcaca acgaaaaatt ttcgtatcta tgccagggca
    62041 atttgagtta ttaattgtat caaagtcagt cagtaccaaa gttggagctg aacccgaccc
    62101 agaccaggaa ataacccgca acgtatcgcc tttatacgca aaagaatcat atttcccata
    62161 tgcaacctcc tttggcgtta tcaccgtaac attgaaaaca ctgctcgcta cagcaccggt
    62221 taccttgtcc atggcataat aaactctcac actgtcaaaa ctcgcactca taagtttatc
    62281 tccagcataa ctcttagatg cgccgacaac actcagaacg agcagcatta ggctcgcgaa
    62341 aattatggtc cattttttca tttcagaaat actcaaaggt tgccgtcact gtggcactaa
    62401 agtctccgga cgttacatct cgcccgctct ttgcctgtaa ccaggtatta aaatttaggc
    62461 tgttatttcc ctgattcagc gggagcgtga cgcctgttgt attgttgatt ggcaccgatg
    62521 tgcctgccgc agtttcaata ccaatcccag cgccggatgc tgatgacgag gagtcaaagg
    62581 ccagaaagcc gggtagagca ctatcttctg ttcctgtcaa cgttactttg acattaaaca
    62641 atgtagaact atgacaatct ttcaattgaa acaccaatgg cactcgctct gactgcccaa
    62701 ggttcattaa atcgctggcg gcaatggcag gaaaatgtac ttctgccaac tggctaccat
    62761 taatcactgg cgtacacgat ttactgatta aattgccggt aaagtgcaaa ttatctcctg
    62821 ctattgtggt tccactgccc agcaacatca ggctactaat aaaaaacaac gtacgctttt
    62881 tcttcattgg taatctacct gtaacgtggc ccaggcctca aagtccgcct ctggcagaat
    62941 gacaccactc ttttttactg gcacagccgt taacaccggt aaatcggttt cattcaccac
    63001 aagtggcgta ttgatcgtaa aactctgccc tgcctgtttg agctgaatgc ctaaaccagt
    63061 gatattgctg cttactgcag caatatcaaa atcgcttggt gtgccactcc agctcaaggt
    63121 catttccatt aatgggtcgc gaaccgccgt atcgcaggta atggaatacg gcacggtttg
    63181 ctcataattg ctgccattaa tgtcatcaat aagcacatcc tgaaaagtta tttcaatggt
    63241 ttgagcgttg ctaatcgtgc agttaggcgg ctgcactaaa taaccgtgaa aagagacgtt
    63301 tgagagtgcc atcgagcaaa agccccacag caaaataagc gagatccgtt tcattggtaa
    63361 tccaccacaa ttgtggcact ggcgttaaat tcagctgccg ctaacgttgt accactctgt
    63421 ttcaccggca cagcttccag aacgggaact cccgaggaaa aattaaatgg caaccatgag
    63481 ccagaggtta agtcgagtat tgtgtgatca ctacttttct gaacccgaat accaaaccca
    63541 ggaatacccg tgctgataac tgtttcaccg ttaatcacga ccgttgtcgc ctgcatctgt
    63601 agacgcaggt catccattgc cagattgtta caaacaagcg agtagtcaat gggacgttta
    63661 taatcaaccc tattgatctt attaatgaat acattaccaa actcaactgc cgcaccattt
    63721 accgtacaag gaggaggggc atcgacaagc acccgtaaat tcatacttac tgacttactc
    63781 gctgcaactg caaagccgct accaacacaa catatcagta acccagttac cgcccagaca
    63841 gaacaagatc ctttcatact ggctctcccg gcctcagcct ttgcggctct gctcttcatc
    63901 gacctagcaa ctgttaccgt tgcaagtgaa aaagagcggc atccgagcgc catagtcgtt
    63961 gacataggtc agtaccggca cgctacccat cttcacattc agcggtgcat aggttttcgg
    64021 cgacatcact attggcgaga aaccggctgc cggattgcca tttttctgtg tgctggcatt
    64081 actgaaaatg acgtaatacg acgtcgggtt attgacggta aattcctgac cgttgcgggt
    64141 taaagtaacc ttatattgct aggggttttt catgctgacg ttctccagcg ccttcggtcg
    64201 ccagaacaac ttaatacgcg tttgtagtgc tatttgcagc gtgttggctt tgtttgattt
    64261 tggcgggatc tcacgcacgt tgaagtagaa caggctttca cgatcggcag gcaatttgtt
    64321 gatatccggc attccctgca cttttacctg cccgttcatc attgaatcga tgcgttgcac
    64381 cggtggcaga acagttaacg gtgaactgat cttgttaccc ttttcatctt cgatccagct
    64441 ttgggccaga taaggcagtt tcggatcgtt attgcgcaac gtcacgctga ttgatttgtc
    64501 gctttcatta aatatcaaac gggtacgatc tggcgttaca gaagcctggg cagacaaact
    64561 ggcagcgcta agcagtaacg ctagcgtcgt agcacccaat ttggcagagc aaagtaaatc
    64621 agacatggat cattccttaa tcacgttatt ggttagctga aacagaaaca gggggattgg
    64681 tgggtgtcac ctgttgcgtc tgctcctgaa tgacaggctt tatgtcatca ggaacaaccg
    64741 gcgcgacgtt gcctttatgc tggcatggca gtaacaagcc gttaaacaga tcggcaggca
    64801 gcgggtccgg caggttgata tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt
    64861 cgccaggttt caccccagcc agataaacat tgccgtcatc atcgacaagg cccactgtct
    64921 gctcgttatc attttttact tctgcgccaa acggaggatg gctgccgtct tgtaaacgca
    64981 gcaccgccat cgctttttga ccactgatga cggcaaattt gcggtagcca atcgcccctt
    65041 cagttagcgt ggcttgtacc accgactggg ttgcttcggc gttttccggc agtttgttga
    65101 ggtcgatata cgcctgattg cggtaatagt tattgacgtc agaaacgacg gctttaccaa
    65161 acatattggt ataaacagca gcaccgttac cttctaccgg aacatcggca acgccatcgg
    65221 catcaatcaa caagcgtgtc ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg
    65281 cagtaagcgt tgcgccgccc tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt
    65341 agttcgcact gaggtcaacc tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc
    65401 tggtgtgttt gtcactggtg ccgacgttca actgatagtg agtcgcgtca tcgacacggc
    65461 tgaaataacc gacctgactg ctgtccgtcc cactgccata gtaccgttat agctaacggt
    65521 actgttgtcg ccccacggca tactgagcga aatgtacatg cctttgtcgg cctggttgtc
    65581 atactcgtaa cggtagccag tcatcgagat gctgacatta cgaatactgc ccatattgaa
    65641 atagtgcgag agcatgatgt tgtagtttgt ctgctcctcg cgatcccagt aggtatggcg
    65701 ggtatagttg agataaaccg aaacacccgc atcgcggaag ttctggttat aagtggcggt
    65761 gtacatctct ttgtcgttgc ccgtgcgtac cattccgctg tcactggcat ccagatactc
    65821 gctcatggtc ataaagttct cttccgagaa gcgatatcca gcaaaagtaa cgcggctgtt
    65881 gagctggtca aagtctttgg aataactcac acggaaggag ttaccgtcca gcgaaccttt
    65941 gccataagcg gtgtctttat ccagtttggt gtgcgagtga gtaacatcaa acgcgaccgc
    66001 gccgaatgta gacaaatcgc gaccgacacc aagcgccgca gactgatagt tttcatctcc
    66061 cagtgcgccg ccatataacg accagccgtt agcgatcccc caggaagctt cagcaccaga
    66121 aaaaaattca ccctcgacat ggtgtcccca ctcttgcgga cggcccatca tgatcttata
    66181 gcgaacctga cctggacgag tgaggtatgg catcgaggcg gtgctgatgt catattcctg
    66241 cacctggccg ttctgttctt caatgcgaat atgcaacgta ccggagacag aatcaccaag
    66301 atcctgaata cgaaacggtc cagccggaac ctgcgtttcg taaatcacac gccccatctg
    66361 gctgacggtc acttttgctg tggtgtgtgc cacgccggaa atgtctggcg cgtagccgcg
    66421 cagattggga ggcaacattt gatcgtcagt actgacactg ccaccaacat agttaaaacc
    66481 atcaaaaata tcggatctga ggtaatcctc gcccagcgcc agtttggctt ttaatgatgg
    66541 taacgcccgc ctggcatagt agcgactcca ctcccatttt ttttgagttt catcgccgct
    66601 gaattcatcg tcatcattac tgcgagtatg ttgatagtta gtctgccagt cagcacgcat
    66661 acgccacggc cctaggttaa ccccaaccgt cccgttgccg ctgatctcgt tactatcatc
    66721 accgccattt tcttcgtgcc gggtttgtgc gttgatgctg tagtccgcga cgatcccgga
    66781 gatgccgtca tcccaacgtg aaggcggatc ccagtcggga taagtatatt cgaggtaagc
    66841 ctgtggcagt gaaatgacta atgcggactg gcttaaatca gccttaattt ccatgccttc
    66901 cagttgaccg gatttcaggc atttagcatc gtggctccat tgcagatttt tcgccacgtc
    66961 ttcttttaaa ccaaactgcg ctaccagttc cggtgtcaga caagcatagc ttttgctcgc
    67021 gtcatcttca ccagcatacc agtaaatatc gtactcttcc gccaatggct gtttatttag
    67081 ttgaacctgt aaattgtatt ttccgggctc aacatatccc tggctggaaa aacgcttcag
    67141 atcaattttt gtgtcgcctt ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc
    67201 agcccagaca ctgctataac tccctgacat tgccagcgca atgcaccacg gaagaatccg
    67261 taatcgaaaa agagaatggt caggcataca cgtttctatc cgtttttatt cttgttcggt
    67321 caaaatccat ttaaatcccc catagcagtg tcgctatggg ggtatgcctt acaggtaagt
    67381 aatctggaag gtggtatttg cttcaaaatt acccagatct ggcgcatcag ctgcgccgac
    67441 cagccaggct ttaaagttca gtgtctgctt cgctttgcct ttgttggtag aagtgtcttt
    67501 cactattttc tggtcaatac ccatgccgct tttgtaagag gtacccagag agtcaccgat
    67561 cgccaggcta acattgttaa atgccgcacc ggtatcggta ttgaaaatgg tgtaataatt
    67621 gccgctattt gcagaagaaa cggtaccagt aaaggtagtg gtcattgttt cctgagtatc
    67681 aaatacgcaa tcttgcagac gaatctggaa acttttcggc gtggtggtgc cgttattttt
    67741 cagtttttcg gcaccgatat cacccatatc aacttccagt ttgtgatcgt ccggaacgat
    67801 agaacacact gagtcttcaa tggtgccgta aaaacgtgca gtaccgttgt tacccgcagc
    67861 gattgttgcc gtagaggtga acacgcccat caccattgcc gcagcaatag ctgttttaac
    67921 aaacttactc attatttgtc cttaaactat aaataattaa aaatataaac attgcaatac
    67981 attgattcag tcaatagcca atgtttatac ccagccattg aatacggcat gcgacccaaa
    68041 aagaacaggt cgtagaaaat tgtattcatt ggtgtggggc cgcatagtga tgtttccaca
    68101 cccctgatcc aatttaattt tactaaagag taaatgtaga tttaattaat atattgacag
    68161 aagaaaggtt atcttaagga catccttaaa aaatacggaa taagtattta gaaaaaatag
    68221 aaagtatatc aataatgaat atcaacagct taaaaaacaa acaatgaaca tataatgcga
    68281 ttggcattta tccacttttg acaatataat atataaacac tatagccaga atttaatcaa
    68341 gtttcaatta catcctgcct gattattcaa ctctattttc tctattatct gtattaataa
    68401 atgacactga gtgtcaaaca gacattattc atttcaacat gtaactttgg gcaaaatcaa
    68461 gatacgcgca gaagaataat ttccgaatga atgtagaaag tgttttattt atagataagg
    68521 agagtcttcg atagtagtga ggttgacaga tatccgctgc ctaatgagca attatgccca
    68581 atgaatctac ctcattgggc ataatttgga acacatcaga tagcttttgc catcttcaga
    68641 ttacacgggc tcatctgcca gttgaatgta ccgtttccgc tttcatcaag cgttacgctg
    68701 gcaatggccg aggtggtaaa cataggcggc gtttcgcccg gacataactc ggcaaccaga
    68761 tacccgacta acggtaagtg agagatcacc agtaccgaag caacaccttc attggtcaac
    68821 gcctgcaaat aggcactgac cagaccaaca tcgccgcagg gcgttaactc cggcagaact
    68881 tctgcactgg aaggcaggtt cagacaatcc cctacttctt ccagtgtttg ctcggctcgc
    68941 aggaacgggc tcaccagaac gcgttcgatt tccacttttt gacctttcag ccagttcgcc
    69001 atcaggcgag attcgtcaca accattagtg gtcagaggac gaaccgaatc actggcggca
    69061 tcgagggctg cgtcgccgtg acgcatgata aaaacttgca tattgcaccg cttttgttaa
    69121 ccagattcgc ccgttttctt tcatctgaaa ccagagaaag aaaacggtgg ccggcattgt
    69181 gccttatcca ttcaccgaat gaaacgctgt tttttacctc aatggcgtaa gtatagtcaa
    69241 tatgcgttta cattttgacc aacactccgc cattcagcgc ggattcatat agctttgacc
    69301 ttcttattgc aggtcagttg cagttgtttt ccaaaaactt tccccacgcg cgcccatctc
    69361 gaccaaacgc tcgcaagggg taaaacggga accatactgc gtggccagtc gttgcattat
    69421 tgcaaccact tcgcccgcgc cgagagaatc gatatagcgg aacggtccac cgagaaatgg
    69481 cggaaaacca atgccaaata ccgcaccaat atccccgtca cgcacgctac ggataacctg
    69541 ctcatcaaca caacgtactg cttcattcag catcaacatc acacaccgtt cagcaacctg
    69601 cggtgcggag agtcgcccct gcccttgtgc gccaatgagc gggtaaatgg caggatcgac
    69661 ctgttttttg cttttacgcc ctttctgacc ataaagatag aaaccccgac cattttttct
    69721 gcctttgcga tcgtcgttca aaattgaaga aacaacattt gcaggcgcgc taaaacgttc
    69781 tccataagcg gcttccagta caggaataat tttagtcccg gtgtcgattc ctacctcatc
    69841 caaaagttgg attgggccta ccggaaaacc aaatttcact agcgcggcat caatgtgctc
    69901 tacccgttca ccttcggtca gcatgcggat agcttcatta atgtaaggcg ctaagatgcg
    69961 attgacgtaa aacccggctt tgtcacgcac gacaatgggc gttttgccct gttttttcgc
    70021 cagttttacc gtagtggcga tggtttgcgc cgatgtaccc gcatgaggaa taatctctac
    70081 cagcggcatt ttttccaccg gactgaagaa atgcaggcca ataacttgct caggtcgcgc
    70141 ggcgtgagcg gcgatatcac caatcggtaa agatgacgtg ttcgaagcaa aaatggtatg
    70201 agcggcgcaa ttttgctcca cttccgccac catctgttgt tttaattcga gattttcaaa
    70261 caccgcttca ataatcagat cgcgatgggc aaagccgcga tagtccgtcg ttccggagat
    70321 taatgccagc tgtttgtcac gttcgctggc tttgagatga cgacggcgaa ctttgccctc
    70381 cagctgatcc caactgtact tcagcgcatg atttatgccc tgcgggttga tatctttaat
    70441 tctgaccgga agccccgctt tacaagcagt gacataagca ataccgccgc ccatcaagcc
    70501 accacctaaa attcccacgc tgtttaatgg cgcaggcggt gcatcactac cgggatcttt
    70561 cttcacgtcc gtactggcaa aaaagatact acgcaaagct tgcgattgcg gcgtcatcgc
    70621 cagttcgcca aaggcgcgag cttcagcgtc atagccgctg ctggtgccct gcgctaatcc
    70681 cgtttcaaca acctccagga tgcgttctgt cgccggataa ttgccttgag ttttgtgttc
    70741 tgttttcttg ccgaccattt tgaacagcag cgcacgacct aacggccccg ccagaatacg
    70801 ctcgcgtaca ggtagagggc gggaagatgg gcgatccttc ttcgccagct caacagcggc
    70861 ttccagcaaa atggagtgcg gaacaacgtc atccaccagc cccagcttta atgcctgttt
    70921 cgcccgaagt tgttttccgg tgagaatcat ctctaatgct gtactgacgc ctatcagacg
    70981 cggtaaacgc tgggtgccgc ctgaaccggg taacaatcca agttgtactt caggcaaacc
    71041 gagcaccgtt ttaggatcgt cagtacaaac gcgaccgtgg cacgccagcg ccagctccag
    71101 cccaccgccc aggcaagcgc catgaatagc cgcgataacc ggaatgggca aagcatgaat
    71161 ctccgccatc aactgttggc cctgccgcgc cagtgcttcc gcttcttgcg ccgttttgca
    71221 gttgccaatc atgttgatgt ctgctccggc gataaaattg tccggtttcg cggagataaa
    71281 caccacgccg cgcaactctt tgttttcacg gagttgctta ataatggcgc gcacctgcga
    71341 ggcaaattcc gccttcaggg tattcatttt ctcatccggt acgtcgatgg tgatgatagc
    71401 aatgttgtcc agacgaacat taagagtaaa cgctgatgcc atttccatta ttccgcctcc
    71461 agaaccattg ccgcgccaag cccaccggca gcacaggcgg taactaaacc aaatccaccg
    71521 ccgcgacggc gaagttcatg caatgtctgg gtaatcatcc gcgcgccggt cgccgcgaag
    71581 ggatgtccgt aagcaatcga accgccaagc acgttaaatt tgctatcgtc cacttcgcca
    71641 gtggcatgtg cacgccccag tacttcacga gcaaaacgtt cactacccag caactgaata
    71701 ttcgccagtg tctgagctgc aaaggcttcg tgcatatcga tcaatgtcag ttcgctcatc
    71761 gtcaaaccgg cacgttccag cgccagcggt gttgaccagg ctggaccgag caacatgtcc
    71821 tgccagacat caatcgcagt aaatgcgtag ctgcgcagat accccagcgg caccagccct
    71881 aattctttcg cccgggattc agtcatcagg atcaccgctg ccgcgccatc ggtcagcggc
    71941 gtactgtttg ccgccgttac cgttccgtgt ttgcgatcaa acgccgggcg cagctttgcg
    72001 taatcggcaa gcgaggaatt accgcgaata ttgttgtctt cgacaagcgg ttgtttataa
    72061 ggagggataa aggcagtcat cacctcttct ttgagttttc cgtctgacca tgcctgagcg
    72121 gcacgctgat gcgaacggtg cgctaatgca tcttgctgtt ctcgggtgat gccgtaggtt
    72181 ttcgccattt gttcggcggt gtcgcccatc cgcaagccag tagaatattc tgctaccgca
    72241 ggcggtacgg gcattaagtc gcgcaaacgc aggcgagaga agagtttcag gcgctggctc
    72301 atggttcgag ctttgttgac atcaaccagc acgtgcgcca gttttttact gacgccaatt
    72361 ggcaatacgg aagaggaatc tgccccaccg gcaatccccg ctcggatagt tcccgccatc
    72421 aggctttctg cgacgtttgc aaccgcctgg aaactggtag cgcaagcgcg gctgacgctg
    72481 taagcatcgg tatgcacatt cattccggta ccgagaacaa tttcacgcgc aatgttgggg
    72541 gcttcaggca tttgtacgac ctgaccaaag accagctgtt caatcacttc agcggggatc
    72601 tcgctgcgtg ccagcagttc gcctaccacc atcttcccta aatcaaccgc gggaatgcca
    72661 tgaaaagccg tcgcctggcg ggcaaaaggc gtacgtaaac cgctaacaat ggcgatacga
    72721 tcgccctggc gggtaaccag tggtaaaacc tgacccataa cactcccctg taaaaaaata
    72781 aataaagtgg tctgacctga tcatagtatt aaccattttt ttacatttag ccaagcggag
    72841 aaaaaggaaa gtgggagcta tgacacagtg aagacagaag aaagaaaaga aaacgcccct
    72901 gccagcaact gacaggggcg tacgtacccg agaggaatta acgcagaccc agctggaaga
    72961 tcagcatttc agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact
    73021 cttcagtgaa agtcggggag attttgcacg gttcggattc cacgctacgg gatttttcag
    73081 tcagcgccgc cagcgtttgt tctgcttctg cgcggtttgc aaacacgcgg ctgtaagacg
    73141 cggtgcagtc ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt
    73201 catcagcact acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat
    73261 aaacgttgtg gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa
    73321 tcggataagt gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatccg
    73381 cgagtgcgca acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa
    73441 aagatggaaa catacttgca acattctagc tggtccgacc tatactctcg ccactggtct
    73501 gatttctaag atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag
    73561 tttgtataaa aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag
    73621 tctgctctcg cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag
    73681 ttaaacgaat tttcttcctc tggcctgggc cgggcttatt caggggaagg cgcaattgcc
    73741 gatgatgcag gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca
    73801 ttttctgcgg gtgcggttta tattgacccg gatgtgaata tcagcggaac gtctccgtct
    73861 ggtcgtagcc tgcaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt
    73921 gttgcaccga ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggtctg
    73981 gcaacagagt ttaatgatac ttatgcaggc ggctctgtcg ggggtacaac tgaccttgaa
    74041 accatgaacc tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt
    74101 ggtttcaacg ccatctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag
    74161 ttggttgctg gtcagattat gcaatctcct gccgggcaaa ctcctcaagg gcaagcattg
    74221 gcagctaccg ccaacggtat cgacagtaat accaaaatcg ctcatctgaa cggtaaccag
    74281 tggggctttg gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca
    74341 ctgacctacc gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat
    74401 cgtgcgttta ataactacgg tttgccaatt cctaccgcga caggtggcgc aacgcaatcg
    74461 ggttatctga cgctgaacct gcctgaaatg tgggaagtgt caggttataa ccgtgttgat
    74521 ccacagtggg cgattcacta tagcctggct tacaccagct ggagtcagtt ccagcagctg
    74581 aaagcgacct caaccagtgg cgacacgctg ttccagaaac atgaaggctt taaagatgct
    74641 taccgcatcg cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt
    74701 atcgcctttg atgacagccc agttccggct cagaatcgtt ctatctccat tccggaccag
    74761 gaccgtttct ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac
    74821 gttggtgttt cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc
    74881 gagtctgaag gtaaagcctg gctgttcggt actaacttta actacgcgtt ctgataacgc
    74941 gttcgcctgg ataaagtcac ctgctatgca ggtgacttta actcccccac ttcaccgaaa
    75001 gtagtttcct cattgcttgc ctcctgagtt ttgcaaacag cctgatggca gcttgccctt
    75061 tcaaatcagt aagtggtatc cacagacaaa cctgaaggga aatgcatatt ttcaagcgtt
    75121 ctgctcgtcc ttctcaaaga gtttactttt ctgcatttcc aggatactca acccccctgg
    75181 ctattgtgcg cttatacact caaattaaag ataggttcta aataaatgag cgttttttga
    75241 tagtctattt cattaggtaa tatatatttg taacaaatca atcaacatag aataaaatca
    75301 tgctaccatc tatttcaatc aacaatacca gcgcagctta cccagaatcc atcaatgaaa
    75361 ataacaatga tgaaattaat ggattagtac aagagttcaa aaaccttttt aatggtaagg
    75421 aaggaataag cacctgtatt aaacatctac ttgagcttat aaaaaacgcc atacgagtaa
    75481 acgacgatcc ttatagattt aatattaata attcctcagt tacttatatt gatattggct
    75541 ccaatgatac agaccatatt actattggta tcgacaacca atagaattac ctgcgaacta
    75601 taaagacaaa gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga
    75661 tatcaataac aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg
    75721 ttttatcttc gataagatat cacacaaact cagacatacg gtaacggaat ttgatgagag
    75781 cgggaaaagc gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcgactc
    75841 tctcgctatt gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga
    75901 tcaggacgac taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata
    75961 ttgtagtgaa aacgccagga gtgcttcagc gtggtttgaa agcagtaaag ctatcatggc
    76021 agaatcattt gcaactggtt ccgatcatca ggttgtaaac gagctcaacg gggaaagact
    76081 gagagaacct aacgaagttt ttaaacgttt aggtcgagca ataagatata attttcaagt
    76141 ggacgatgca aaatttagac gcgataatgt aaaagaaata atttctactt tattcgctaa
    76201 caaagttgac gttgaccatc ctgaaaataa atacaaagac tttaaggatc tcgaagataa
    76261 agttgaaaaa agacttcaaa attgccaggc gaaatatcaa aatgaaatta atcaactatc
    76321 tgcactaggt gttaattttg atgatattta aaattatttt tccagttttt tgatttttca
    76381 acgcctgaat atcggaaata gtcagtgaca aa
//
LOCUS       DADLZM010000005        65471 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLZM010000005 DADLZM010000000
VERSION     DADLZM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.005"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(127..606)
                     /gene="folK"
                     /locus_tag="JNY73_000341"
     CDS             complement(127..606)
                     /gene="folK"
                     /locus_tag="JNY73_000341"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY8046472.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="JNY73_000342"
     CDS             complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="JNY73_000342"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY8046473.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="JNY73_000343"
     CDS             complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="JNY73_000343"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY8046474.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3023..3478)
                     /locus_tag="JNY73_000344"
     CDS             complement(3023..3478)
                     /locus_tag="JNY73_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY8046475.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="JNY73_000345"
     CDS             complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="JNY73_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY8046476.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="JNY73_000346"
     CDS             complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="JNY73_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY8046477.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4979..7408
                     /gene="hrpB"
                     /locus_tag="JNY73_000347"
     CDS             4979..7408
                     /gene="hrpB"
                     /locus_tag="JNY73_000347"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY8046478.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAQLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7604..10138
                     /gene="mrcB"
                     /locus_tag="JNY73_000348"
     CDS             7604..10138
                     /gene="mrcB"
                     /locus_tag="JNY73_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY8046479.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10358..12601
                     /gene="fhuA"
                     /locus_tag="JNY73_000349"
     CDS             10358..12601
                     /gene="fhuA"
                     /locus_tag="JNY73_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY8046480.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12652..13449
                     /gene="fhuC"
                     /locus_tag="JNY73_000350"
     CDS             12652..13449
                     /gene="fhuC"
                     /locus_tag="JNY73_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY8046481.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13449..14339
                     /gene="fhuD"
                     /locus_tag="JNY73_000351"
     CDS             13449..14339
                     /gene="fhuD"
                     /locus_tag="JNY73_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY8046482.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14336..16318
                     /gene="fhuB"
                     /locus_tag="JNY73_000352"
     CDS             14336..16318
                     /gene="fhuB"
                     /locus_tag="JNY73_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY8046483.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLIMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="JNY73_000353"
     CDS             complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="JNY73_000353"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY8046484.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17858..19279
                     /gene="clcA"
                     /locus_tag="JNY73_000354"
     CDS             17858..19279
                     /gene="clcA"
                     /locus_tag="JNY73_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY8046485.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19303..19368
                     /locus_tag="JNY73_000355"
     CDS             19303..19368
                     /locus_tag="JNY73_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046486.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19361..19705
                     /gene="erpA"
                     /locus_tag="JNY73_000356"
     CDS             19361..19705
                     /gene="erpA"
                     /locus_tag="JNY73_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY8046487.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19752..20375)
                     /locus_tag="JNY73_000357"
     CDS             complement(19752..20375)
                     /locus_tag="JNY73_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY8046488.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREISMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="JNY73_000358"
     CDS             complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="JNY73_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY8046489.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="JNY73_000359"
     CDS             complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="JNY73_000359"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY8046490.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21988..23505
                     /gene="dgt"
                     /locus_tag="JNY73_000360"
     CDS             21988..23505
                     /gene="dgt"
                     /locus_tag="JNY73_000360"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY8046491.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23635..25059
                     /gene="degP"
                     /locus_tag="JNY73_000361"
     CDS             23635..25059
                     /gene="degP"
                     /locus_tag="JNY73_000361"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY8046492.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25214..26370
                     /locus_tag="JNY73_000362"
                     /pseudo
     CDS             25214..26370
                     /locus_tag="JNY73_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26459..26845)
                     /locus_tag="JNY73_000363"
     CDS             complement(26459..26845)
                     /locus_tag="JNY73_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY8046493.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="JNY73_000364"
     CDS             complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="JNY73_000364"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY8046494.1"
                     /translation="MQQLQNIIETAFERRADITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAIIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="JNY73_000365"
     CDS             complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="JNY73_000365"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY8046495.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWHIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30748..31542)
                     /gene="map"
                     /locus_tag="JNY73_000366"
     CDS             complement(30748..31542)
                     /gene="map"
                     /locus_tag="JNY73_000366"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY8046496.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31910..32635
                     /gene="rpsB"
                     /locus_tag="JNY73_000367"
     CDS             31910..32635
                     /gene="rpsB"
                     /locus_tag="JNY73_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY8046497.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32893..33744
                     /gene="tsf"
                     /locus_tag="JNY73_000368"
     CDS             32893..33744
                     /gene="tsf"
                     /locus_tag="JNY73_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY8046498.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33891..34616
                     /gene="pyrH"
                     /locus_tag="JNY73_000369"
     CDS             33891..34616
                     /gene="pyrH"
                     /locus_tag="JNY73_000369"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY8046499.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34908..35465
                     /gene="frr"
                     /locus_tag="JNY73_000370"
     CDS             34908..35465
                     /gene="frr"
                     /locus_tag="JNY73_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY8046500.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35557..36753
                     /gene="ispC"
                     /locus_tag="JNY73_000371"
     CDS             35557..36753
                     /gene="ispC"
                     /locus_tag="JNY73_000371"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY8046501.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36942..37700
                     /gene="ispU"
                     /locus_tag="JNY73_000372"
                     /gene_synonym="uppS"
     CDS             36942..37700
                     /gene="ispU"
                     /locus_tag="JNY73_000372"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY8046502.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37713..38570
                     /gene="cdsA"
                     /locus_tag="JNY73_000373"
     CDS             37713..38570
                     /gene="cdsA"
                     /locus_tag="JNY73_000373"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY8046503.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38582..39934
                     /gene="rseP"
                     /locus_tag="JNY73_000374"
     CDS             38582..39934
                     /gene="rseP"
                     /locus_tag="JNY73_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY8046504.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39964..42396
                     /gene="bamA"
                     /locus_tag="JNY73_000375"
     CDS             39964..42396
                     /gene="bamA"
                     /locus_tag="JNY73_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY8046505.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42518..43003
                     /gene="skp"
                     /locus_tag="JNY73_000376"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42518..43003
                     /gene="skp"
                     /locus_tag="JNY73_000376"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY8046506.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43007..44032
                     /gene="lpxD"
                     /locus_tag="JNY73_000377"
     CDS             43007..44032
                     /gene="lpxD"
                     /locus_tag="JNY73_000377"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY8046507.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44137..44592
                     /gene="fabZ"
                     /locus_tag="JNY73_000378"
     CDS             44137..44592
                     /gene="fabZ"
                     /locus_tag="JNY73_000378"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY8046508.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44596..45384
                     /gene="lpxA"
                     /locus_tag="JNY73_000379"
     CDS             44596..45384
                     /gene="lpxA"
                     /locus_tag="JNY73_000379"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY8046509.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45384..46532
                     /gene="lpxB"
                     /locus_tag="JNY73_000380"
     CDS             45384..46532
                     /gene="lpxB"
                     /locus_tag="JNY73_000380"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY8046510.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46529..47125
                     /gene="rnhB"
                     /locus_tag="JNY73_000381"
     CDS             46529..47125
                     /gene="rnhB"
                     /locus_tag="JNY73_000381"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY8046511.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47162..50644
                     /gene="dnaE"
                     /locus_tag="JNY73_000382"
     CDS             47162..50644
                     /gene="dnaE"
                     /locus_tag="JNY73_000382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY8046512.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50657..51616
                     /gene="accA"
                     /locus_tag="JNY73_000383"
     CDS             50657..51616
                     /gene="accA"
                     /locus_tag="JNY73_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY8046513.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51715..53856
                     /gene="ldcC"
                     /locus_tag="JNY73_000384"
     CDS             51715..53856
                     /gene="ldcC"
                     /locus_tag="JNY73_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY8046514.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53913..54302
                     /locus_tag="JNY73_000385"
     CDS             53913..54302
                     /locus_tag="JNY73_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY8046515.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54367..55665
                     /gene="tilS"
                     /locus_tag="JNY73_000386"
     CDS             54367..55665
                     /gene="tilS"
                     /locus_tag="JNY73_000386"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY8046516.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55714..55974)
                     /gene="rof"
                     /locus_tag="JNY73_000387"
     CDS             complement(55714..55974)
                     /gene="rof"
                     /locus_tag="JNY73_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY8046517.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55961..56161)
                     /locus_tag="JNY73_000388"
     CDS             complement(55961..56161)
                     /locus_tag="JNY73_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY8046518.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56327..56872
                     /locus_tag="JNY73_000389"
     CDS             56327..56872
                     /locus_tag="JNY73_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY8046519.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56869..57291
                     /gene="arfB"
                     /locus_tag="JNY73_000390"
     CDS             56869..57291
                     /gene="arfB"
                     /locus_tag="JNY73_000390"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY8046520.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57305..58015
                     /gene="nlpE"
                     /locus_tag="JNY73_000391"
                     /gene_synonym="cutF"
     CDS             57305..58015
                     /gene="nlpE"
                     /locus_tag="JNY73_000391"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY8046521.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58170..58994)
                     /locus_tag="JNY73_000392"
     CDS             complement(58170..58994)
                     /locus_tag="JNY73_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY8046522.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRSIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59047..60765)
                     /gene="proS"
                     /locus_tag="JNY73_000393"
     CDS             complement(59047..60765)
                     /gene="proS"
                     /locus_tag="JNY73_000393"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY8046523.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="JNY73_000394"
     CDS             complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="JNY73_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY8046524.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="JNY73_000395"
     CDS             complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="JNY73_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY8046525.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="JNY73_000396"
     CDS             complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="JNY73_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY8046526.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62957..63610)
                     /gene="metI"
                     /locus_tag="JNY73_000397"
     CDS             complement(62957..63610)
                     /gene="metI"
                     /locus_tag="JNY73_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY8046527.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63603..64634)
                     /gene="metN"
                     /locus_tag="JNY73_000398"
     CDS             complement(63603..64634)
                     /gene="metN"
                     /locus_tag="JNY73_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY8046528.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64822..65397
                     /gene="gmhB"
                     /locus_tag="JNY73_000399"
     CDS             64822..65397
                     /gene="gmhB"
                     /locus_tag="JNY73_000399"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY8046529.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15463 a        16220 c        18045 g        15743 t
ORIGIN      
        1 gggattgata tttatctgcg ggaatttata ttatgtttct gcaaaaatgt ttaaactaaa
       61 atttttatta aggaaagaga ggatgtatga ttttcagaaa atatttatat tcgtaatata
      121 aataaattac catttgctta atttgtcaaa tgctcttgta tgtaagactt cacgcaacgt
      181 ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc
      241 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc
      301 aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg
      361 accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgggcgaagt
      421 ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt
      481 gcggtaaaac gaagaaacgg caagaatgcg gctttcaggg atatcgccta atgctttcag
      541 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      601 tgtcatgtgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acgacgacgc
      661 ggtgacggtt cttcatccag ctcgttaagc atcccttttt gatctggtgg tgcggaaacc
      721 tggaactcac cccaccattt caccagacgc tgcagttcag cattacgttc aacttcagct
      781 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
      841 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      901 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      961 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
     1021 agcagtgggt accagaacat ggcggcaaac aagaacgccg ggttcacgcg catatcgtta
     1081 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1141 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1201 cacaacagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1261 aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg
     1321 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1381 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1441 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
     1501 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1561 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1621 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
     1681 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1741 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1801 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1861 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac tgtcacctgt
     1921 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1981 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     2041 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     2101 actcgggcac ggcggtcagc atccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2161 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     2221 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2281 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2341 ggtacagcga gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     2401 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     2461 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca
     2521 gcaggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     2581 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     2641 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     2701 gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     2761 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2821 cttctatgcg taccagccag cgaccttgcc gggcgcgacc ctgcaaatag ctgccgagcg
     2881 cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2941 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     3001 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     3061 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3121 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3181 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3241 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3301 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3361 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3421 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3481 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3541 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3601 gaaaagcgta ttcgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3661 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3721 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3781 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3841 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3901 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3961 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttcggagc
     4021 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     4081 ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata aatgcgccgc
     4141 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4201 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     4261 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4321 gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg
     4381 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4441 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cccggcggcg ggaatgtcac
     4501 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4561 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4621 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4681 cgtgagtgtg aaacctggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4741 ctctgcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4801 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcgggtttc
     4861 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4921 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4981 gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc
     5041 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     5101 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     5161 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5221 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5281 aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg gattggtgat
     5341 ccttgatgag tttcatgagc gcagtttgca ggcggatttg gcgttggcgc tgttactcga
     5401 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa
     5461 tgaccgcttg cagcaaatgc tgccggaagc gcccgtcgtc atctcagaag ggcgctcgtt
     5521 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg aagccgttgc
     5581 ggtagccacc gccgaaatgg tgcgtcagga aagtggatca ttactgttat ttttacctgg
     5641 cgtcggagaa attcagcgcg tgcaggaaca actggcttcg cgcatcggca gtgatgtggt
     5701 gctctgcccg ctgtatggcg cgttgtcact gaacgatcag cgaaaagcga tcctcccggc
     5761 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5821 cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgct ttgatccgcg
     5881 cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga cgcagcgtgc
     5941 cgggcgcgcc gggcgtctgg agccgggtat cagcctgcat ttaatcgcca aagaacaagc
     6001 agaacgcgcc gcggcgcaaa gtgagccgga gatcttacaa agcgatcttt ccggtttgct
     6061 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6121 gccaacggtg aatctactgg ccgcgaaacg cctgttacaa atgctggggg cgctggaggg
     6181 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6241 ggcaatgctg gttaacgcaa agagcgatga cgaagctgct accgcggcaa aaattgccgc
     6301 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6361 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgcggcgg
     6421 tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc
     6481 tcatcgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga
     6541 tgccgacgac gcgctaagcc gccacgaatg gttgatcgca cagttattat tgcagggcag
     6601 cgcctcgccg gatgcgcgga ttttactggc gctaccggtc gatattgatg aattagtaca
     6661 acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg cgcaaggtac
     6721 gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg gtgaaagtgc agccgctggc
     6781 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg ataaaggttt
     6841 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6901 aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttgt tggcgacgct
     6961 ggaaacgtgg ctgctgccac atatgaccgg cgtacattca ctacgcggcc tgaaatcact
     7021 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     7081 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7141 agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg ccactaatcc
     7201 gacgatcgcc caggggcgcg tgccgctggt gctgaagttg ctttcccctg cccaaaggcc
     7261 gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg aggtgcaaaa
     7321 ggagatgaaa gggcgttatc ccaaacacgt ctggccgaac gacccggcaa acacggcacc
     7381 gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg
     7441 gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc cctcaaggga
     7501 gaggggaccg atcgagcaca aattttgaga gatatcttct tctgtcttgt aacagaagaa
     7561 cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg
     7621 cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg
     7681 tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat
     7741 gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct
     7801 atggctactg ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta
     7861 tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc ccgcggcagt
     7921 ttatggccga atggtcaacc ttgaaccaga tatgaccatc agcaagaacg aaatggtgaa
     7981 gctgctggag gcaacacagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac
     8041 cgtgcaggcc aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga
     8101 agggcaggtg cgtgcgcgtc tgacctttga tggtgatcat ctggcgacca tcgtcaatat
     8161 ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc
     8221 ctcgccaaac ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg atttgctggt
     8281 ggatactttg ctggcgacag aagaccgtca tttttacgag catgatggaa tcagtctcta
     8341 ctccattggg cgtgcggtgc tggcaaacct gaccgcaggt cgcacggtgc agggtgcgag
     8401 tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgtt cttactggcg
     8461 taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat
     8521 tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg
     8581 cttcccgctg gcaagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca
     8641 gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc
     8701 gaaactggcg ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat
     8761 tgatcaagaa ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc cgcgtggtgg
     8821 ggtgatctct cctcagccag cctttatgca gttggtgcgt caggagttgc aggcaaaact
     8881 gggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc
     8941 ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa
     9001 gttgagcgat cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc
     9061 gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg
     9121 ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc agccgaaaat
     9181 ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca
     9241 ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt
     9301 ggatgcgttg acacgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct
     9361 gcctgcggtt acggagacct ggatcaaact gggcgtaccg aaagatcagt tgcatccggt
     9421 tccggcaatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcattcca
     9481 gaccatcgcc agcggtggta accgtgcacc gctttctgca ctgcgttcgg taatcgcgga
     9541 agatggtaag gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc cggcgcaggc
     9601 ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct
     9661 tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt
     9721 agatacctgg tttgcaggca ttgacggcag cacggtgacc atcacctggg taggccgtga
     9781 taacaaccag ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta
     9841 tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat
     9901 gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg
     9961 gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa
    10021 tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga
    10081 gcagaaagac agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca
    10141 tctaagcgtg aaataccgga tggcgagttg ccatccggta aaataacatc ctatctaaga
    10201 tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt
    10261 accgcttgcg aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg
    10321 tttacgttat cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca
    10381 gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc
    10441 tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc
    10501 tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgcggc agtcagctac
    10561 cggcactaaa accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc
    10621 cgaagagatg gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg
    10681 tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac catctgatca ttcgcggttt
    10741 tgcggcagaa ggccaaagcc aaaataacta tctgaatggc ctgaagttgc agggcaactt
    10801 ctataacgat gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc
    10861 ggtttccgtg ctttacggta aaagcagtcc tggcggtctg ttgaatatgg tcagcaagcg
    10921 tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca
    10981 gactggtttt gactttagcg atgcgttgga tgatgacggt gtttactctt atcgcctgac
    11041 cggtcttgcg cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat
    11101 tgcaccggcg ttcacctggc gtccggatga taaaaccaat ttcaccttcc tttcttactt
    11161 ccagaacgag ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc
    11221 gctgccgaac ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta
    11281 ttctcgtaat gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac
    11341 tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta
    11401 cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc
    11461 ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt
    11521 ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct
    11581 gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga
    11641 ctctgtgcca ctgctcaatc tgtacaatcc ggtgaatccc gatttcgact tcaatgccaa
    11701 agatccggca aactccggcc cttaccgcat tctgaataaa cagaaacaaa cgggcgttta
    11761 tgttcaggat caggcgcagt gggataaagt gctggtcacc ctaggcggtc gttatgactg
    11821 ggcagatcaa gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca
    11881 gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag
    11941 ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc
    12001 gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt
    12061 agttactggt gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg
    12121 ttccttcttc tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa
    12181 agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata
    12241 caccaccgat actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc
    12301 gctgtgggct gactatacct tctttgacgg tccgctttca ggtttgacgc tgggcaccgg
    12361 tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta
    12421 tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa
    12481 cgtggcgctg catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac
    12541 ttatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct ttcgtttcta
    12601 atttctcttt tggggcacgg atttccgtgc ccatttcaca agttggctgt tatgcaggaa
    12661 tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg
    12721 cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt
    12781 ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggtcgtca tcagcctccg
    12841 tcagaagggg agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt
    12901 gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat gaccgtgcgt
    12961 gaactggtgg cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggtgcggca
    13021 gatcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg
    13081 ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg
    13141 caggatagcc gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag
    13201 gttgatgtgc tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc
    13261 gtgttgcacg atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcagt
    13321 ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg
    13381 atttatggca tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt
    13441 gtttattgat gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc
    13501 tttctccgtt gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta
    13561 ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg
    13621 gcgtggcgga taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag
    13681 tgattgacgt cggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt
    13741 cgtttatggt ctggtcggca ggatatggcc cttcatcaga aatgctggca cgtattgcgc
    13801 cgggtcgcgg atttaacttc agtgacggca aacatccgtt ggcgatggcg cgtaaatcgc
    13861 tgacggaaat ggcagattta cttaacctgc aaagcgcagc ggaaacgcat ttagcgcaat
    13921 acgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac
    13981 tgctgacgac gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc
    14041 aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg
    14101 gcagtaccgc cgtcagtatc gatcgtctgg cagcgtataa agacgttgat gtgctctgtt
    14161 ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca
    14221 tgccgtttgt ccgtacaggg cgctttcagc gcgtacccgc agtctggttt tatggtgcaa
    14281 cgctttcggc aatgcacttt gtgcgtgttc tggataacgc catcggaggt aaagcgtgag
    14341 taaacgaatt gcgcttttcc cggcgttatt gctggcgctg ttagtgattg tcgctacggc
    14401 gctcacctgg atgaacttct cacaggcgct gccgcgcagt cagtgggcgc aggccgcctg
    14461 gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct
    14521 ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt
    14581 actgcgtaac ccgctggcgg aaccgacgac gttgggcgtt gcaacaggcg cgcaactggg
    14641 gattaccgtc accacgctct gggcgatccc tagcgcgatg gcgagccagt ttgctgcgct
    14701 ggcaggggct tgcgttgttg gcttaattgt ctttggcgtc gcgtggggga aacggctgtc
    14761 gccggtaacg ctgattctcg cggggttggt agtgagcctt tattgcggcg caatcaatca
    14821 gttactggtt atcttccatc atgaccagtt gcaaagcatg tttctgtgga gcactggaac
    14881 gctgacgcaa accgactggg gcggtgttga gcgtttatgg ccgcagttgc tgggcggtgt
    14941 gatgctgacg ttgctgctac ttcgtccgtt aaccctgatg gggcttgatg atggcgtggc
    15001 gcgcaatctc gggctggcct tgtcgcttgc gcgtctggcg gcgctgtcgc tggcgattgt
    15061 catcagtgcg ctgctggtga acgctgtggg gattatcggc tttatcgggt tgttcgcgcc
    15121 gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc tggcgtcgtt
    15181 gattggtgcg ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg
    15241 gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct
    15301 gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gttaacgatc gtgtcgcgac
    15361 tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgttgt tgatggctgt
    15421 ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt
    15481 gctcgaggat ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt
    15541 catgctggcg gtagcgggct gtattattca gcgactgacc ggcaacccga tggcaagccc
    15601 ggaagtgctg gggattagct ccggcgcggc gtttggtgtg gtgttaatgc tgtttctggt
    15661 accgggtaat gcctttggct ggttgttacc cgcaggcagt ctcggggcgg cggtgatgct
    15721 gttgatcatt atgatcgccg ccggacgcgg tggattttcc ccacaccgca tgttactggc
    15781 ggggatggcg ttaagtaccg cgttcaccat gcttttgata atgttgcagg caagtggtga
    15841 cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc
    15901 tcaggtctgg cgcaccggaa ttgtgatggt gattttgctg gcgattactc cgctgtgccg
    15961 ccgctggctg accattttac cgctgggtgg tgataccgcg cgggcggtag ggatggcgct
    16021 gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat
    16081 gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg
    16141 ctttcgacgg acgatgccac atatcgtaat ttcggcgctg gtgggtggtt tactgctggt
    16201 gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatcccgg cggggctgct
    16261 gtcgaccttt atcggtgcgc catattttat ctatttgctg agaaagcaga gccgttaata
    16321 gaatgcctcg cccggtgggc gaggcaaaat tatcacaact tcgcgaacac ccgacgtgca
    16381 gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cagacataaa gcccgcttca
    16441 aacgctgacg gtgccaggta aacaccttca tccagcatca tatggaagaa acgcttaaaa
    16501 cgctcgacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16561 aagataccga acataccacc gacgtggtta acgaccagcg gaattcccgc ttcttctgcc
    16621 gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaatgccc
    16681 ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaatcgg gttaccggaa
    16741 agcgtacccg cctgatagac cggacctgtc ggggccagcg catccattac atcacgacga
    16801 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt ttccgaggca ggttaaatct
    16861 ggcactacgc cgaaataatc ctgtgcgcca gctagcgcta cgcggaaacc ggtcatcact
    16921 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cacgcagacc tggcaggaac
    16981 tctggcagcg gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca
    17041 atctcttgcg ggtattgctc aaacgcggcg cgtacagagg ccagatcgtt ataagtacag
    17101 gttaaggtat gtttggcgaa atctgccgga acgcccggcg agtttggctg gcctaacgtg
    17161 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca
    17221 aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc
    17281 gcctcggtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17341 agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg
    17401 gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat cggcccccag
    17461 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17521 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17581 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt
    17641 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17701 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17761 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17821 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    17881 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    17941 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttatcgggct
    18001 ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct
    18061 ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt
    18121 gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag ggggatcggg
    18181 gatcccggaa attgaagggg cgctggaaga tcaacgtcct gttcgctggt ggcgtgtatt
    18241 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga
    18301 aggaccaacc gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct
    18361 gaaaggtgat gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc
    18421 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18481 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18541 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc
    18601 gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18661 ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18721 taccaaatgg gtactgatgg ggggggcgat tggcggtctg tgtggattgc tggggtttgt
    18781 ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt
    18841 cagcatggga atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt
    18901 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    18961 aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac
    19021 gtttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg
    19081 gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac
    19141 cggtcttggc gcaacactat tagcgcaatt taccggcggg aaacctctat actcggcgat
    19201 tcttgcgcgc acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19261 agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19321 ttagaatttg cccgattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19381 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19441 taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19501 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19561 tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactacac
    19621 cgaaggactg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19681 ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac tatccggcac
    19741 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19801 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19861 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    19921 gttgcgtaga tttctgtacg taaaatcatg gagatttcgc gggccagtac atcacgaatg
    19981 atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc
    20041 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    20101 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    20161 ctggtgacca tggcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    20221 tccagcgcca tgtcgcgaat tgttccgtcg cctactgcgg taaccacgcc cagtaccaga
    20281 acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc
    20341 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga
    20401 aaaggtcagg cactaatcta cctgtgaaag cgcattacag agctgttgtg ctgcgaggat
    20461 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20521 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20581 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20641 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt
    20701 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20761 agcatattgc gctttcaatt gcacgtactg atccagcagg gattgcgcgg cttgttcggc
    20821 cttgtctggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    20881 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    20941 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    21001 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    21061 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    21121 gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agtggcgcga gaaaagacag
    21181 ggcgaccagc gccctgaaca gtgatttagc catgtgccag tttctgcacc agtgactcaa
    21241 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21301 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21361 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21421 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21481 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21541 cagctttaaa gcctgtcgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21601 catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact ttcagcgttg
    21661 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21721 gcagagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21781 cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt
    21841 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21901 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21961 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    22021 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    22081 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    22141 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22201 gaagtccagc aggtgggacg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22261 aaattgctgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag cattgttgag
    22321 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg
    22381 ataaatgact ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca gcctctgact
    22441 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22501 ctgcggcgta agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22561 ctggtgcata cattgatgcg gatgaatctc acttgggcac aggttggcgg tattttaaaa
    22621 tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22681 aagccgggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22741 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc
    22801 tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt
    22861 tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22921 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    22981 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    23041 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    23101 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtattt
    23161 agccatccgg atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23221 gagatttatc gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa
    23281 gaacgggtga aacgtttccc tattgagtcg cgcttattcc acaaactctc gacgcgccat
    23341 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23401 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23461 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23521 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg ggctataaaa
    23581 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23641 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23701 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23761 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23821 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23881 tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat
    23941 ggcggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    24001 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag
    24061 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    24121 gcgctgatcc aaatccagaa cccgaaaaaa ctgaccgcaa ttaagatggc ggattctgat
    24181 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct gggcgagacg
    24241 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga aaactacgaa
    24301 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24361 ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc
    24421 ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24481 tacgggcagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg
    24541 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24601 tcctcggctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24661 ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24721 ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga actgcagcag
    24781 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg cgctgagatg
    24841 agcaacaaag gtaaagatca gggcgtggta gtgaacaacg tgaagacggg cactccggct
    24901 gcgcagatcg gcctgaagaa aggtgatgtg attattggtg cgaaccagca ggcagtgaaa
    24961 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    25021 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    25081 atacgggaag gggttctcct tacaatctgt gaacttcgcc acaactccat acatcttcat
    25141 catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25201 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25261 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25321 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25381 acagggacga gtcgtcgata tcgatgacgc ggtagcacgt catctgcacg gtgtgcggca
    25441 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25501 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25561 ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg aagaactggt
    25621 gatgaacctg attcaggcag aggagaatac tcccgcactt ctgaatgggc gcaacggctg
    25681 gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt
    25741 ggcgtggaca gcgcaatggc ggagttacaa caactgcaaa acgcgctgac tacgcccgag
    25801 cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg
    25861 aactcttttg ggcgctggga tgcagaagat catcgtaagc gagttgaaca actgattacc
    25921 cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac tgggcaacta ttttaccggt
    25981 cctagcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag
    26041 cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac
    26101 agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg
    26161 atggacaata acggcttgct gcgacgaacg ctggcggcgt ggtttcgcca caatgtgcaa
    26221 ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata ccctggagta tcggcttaat
    26281 cgtatatcgg aactgaccgg gcttgatttg ggcaattttg atgacaggtt gctgctgtat
    26341 gtggcgttac aactggatga agagcggtag attatgcgtt aaggttgagg cggtgctggc
    26401 tcattcccct cagcccgaag gggagagggg aatgcccgag ttaaccgtca attcgggatt
    26461 atttacgcgt tagtttttcc agatcggctt caatctcgct gatcttattg gttacgactg
    26521 actccaggtg acgtaagtcg tcgaggatct tacgcttaag atcaacttcg ctgcggtcac
    26581 gctgacagat ttgatcaagc tcatcaatga tataccgtag attcgggctg atttcctgga
    26641 cttctttata accctgaccc acaccatcag cgacgaccgt tttacgctga cgcggatatt
    26701 taaacttaac gctcttggcg aaaaactcgc ctttgtcttt ctggaaatag attttcagaa
    26761 tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat ttcttcagga ttggtaatac
    26821 ccagactttt cagattgtcg tacatagcgt tacctcaaaa tgagtcagta aattgtgctt
    26881 attttagcat ttggcttggc ccgcccggct cgactgtgat cgcagaaagc ctttttcttc
    26941 cagacttcta tcatggcgta aaaatcaaaa aattacctgc tttattgtgg cgatattcca
    27001 tgggatggat tagcacattt atcttcgttt tgcgaattac atgacagtcc gaaacaagat
    27061 gaaaaagtat ttaaaaaagg gggaggaaga atgagtagga cgaattattc tgagccagaa
    27121 agcatacggg cagcatcatg ctgcccgtat atgtactctt aatcgatggt acgcagcagt
    27181 tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aatcgcacag
    27241 tagaggctgt atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc
    27301 ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca
    27361 atgtatacgc ccatagagat gacggaacct tcttcgacaa tcaccccttc aaccacttca
    27421 gagcgtgcgc caatgaagca gttatcttca ataatggtcg ggttagcctg cagcggctcc
    27481 agcacgccgc cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa
    27541 gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgtta
    27601 acgtaagacg gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct
    27661 ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc
    27721 atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata
    27781 cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt
    27841 ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg
    27901 cgggtaacgg tgtctgcatt ggctggcgtg atatcggcac ggcgttcaaa agcggtttca
    27961 ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct
    28021 ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta
    28081 ttaagcgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg
    28141 gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactcgc
    28201 gccagcagtc caggttggtc gagggcgatc agttcgagga acgatttgcg gtcggtatgg
    28261 gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga
    28321 cggggctgcg gtggctgcca gctactttgc gtcagcacct gctccagacc aaaccgaata
    28381 acctcatggc gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc
    28441 atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg
    28501 tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc
    28561 tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca gcggtttgct taaatcatgc
    28621 tgtaataaat ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta
    28681 gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt
    28741 agtgccagtg cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc
    28801 attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc
    28861 cagctattcc acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga
    28921 tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc
    28981 tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca
    29041 accagctgtg tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga
    29101 gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg
    29161 atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc
    29221 tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca
    29281 tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc
    29341 ggcatatacg cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct
    29401 ccggggtgac gcagaatgct caaaaacagt tttcgtgctt gcggaatatt acacagcggt
    29461 tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aataccggtg
    29521 atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata
    29581 aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgagt
    29641 ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc
    29701 atctggttga gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg
    29761 accggttcgt tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg
    29821 aataacagac gattatcgta acggctgacg accagatgca gggcaaagcg aatatgccac
    29881 agtatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaacccg
    29941 accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtga
    30001 atatcgcgca agccgccagg gctacttttg atgtctggtt caaggttgta gctggtgcca
    30061 tggtaacgct gatggcgctg gttctgttct tcaactttcg ctgcgtagaa cttgtcggaa
    30121 ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca
    30181 attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa cccttccaac
    30241 atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc
    30301 gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc
    30361 agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcaacc
    30421 aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg
    30481 tctatgaact cggtacgtgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca
    30541 tcacccagcc aacgctggaa agtatcgata tgggctttta tcccacagac ggttaattca
    30601 tcacggggcc aggcgcatgg attttgcggt tgaccgggca gggtggagag agcggtgttt
    30661 gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat tatcgcatta
    30721 aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt
    30781 gtcatccttg cgtagcgtca aaatttcgca gccgttatca gtcaccacaa tagtatgctc
    30841 atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg
    30901 gatctctttt ttacccgcgt tgaccattgg ctcaatggtg aacgtcatcc ccggtttcag
    30961 cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg
    31021 accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaattt
    31081 ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc gtagcgccag
    31141 gtacaggctt tcttgcgtga tgcggcacag gcgttcgccc atgatggtcg gcttaccgac
    31201 gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt
    31261 aacgatatcg ccatctttca gcagcttagc atcgtccggg ataccgtggc acaccacttc
    31321 attaatagag atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc
    31381 gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc
    31441 cggtttaaca tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg
    31501 cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg
    31561 tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg
    31621 agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata gaaaattcga
    31681 cgtctgatgc tgtacacagc gccaacaatt attggtgtcc acgacgtatt tgtggtataa
    31741 agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac
    31801 tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat
    31861 atgggatacg tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt
    31921 ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa
    31981 cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga
    32041 gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg tttctcgcaa
    32101 aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc
    32161 tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg
    32221 gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg
    32281 tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact
    32341 ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat
    32401 cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacttgggta ttccggtatt
    32461 tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga
    32521 cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg
    32581 ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg
    32641 cttgataact cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg
    32701 ggagcttaca tcagtaagtg accgggatga gcgagcgaag acaacgcatc tgcggcgcga
    32761 aatatgaagg gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca
    32821 tatggccccc tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc
    32881 gaggatttta gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact
    32941 ggcgcaggca tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg
    33001 gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt
    33061 gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt
    33121 aactgccaga ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt
    33181 ctggacgcag ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa
    33241 gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct
    33301 gcgctggaag gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt
    33361 gctgctaaag gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc
    33421 aagccagaat tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag
    33481 gtacagctgg atatcgcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt
    33541 gaaggccgca tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg
    33601 gaaccaagca aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc
    33661 atccgtttcg aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt
    33721 gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt
    33781 tgtgcccatc ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa
    33841 tgtcgctagt attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca
    33901 atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca
    33961 ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac
    34021 tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg
    34081 ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga
    34141 ccgtaatgaa cggcttggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc
    34201 tgatgtccgc tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca
    34261 gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta
    34321 ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag
    34381 caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt
    34441 acgagcaatt gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg
    34501 ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg
    34561 gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc
    34621 cgtgatggat aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta
    34681 taaggcgtta ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca
    34741 aaagtacgtg aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat
    34801 aatccagacg attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt
    34861 gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata
    34921 tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca
    34981 gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt
    35041 acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc
    35101 tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcct
    35161 ccgatctcgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc
    35221 tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc
    35281 gtgttgcagt acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag
    35341 ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg
    35401 atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt
    35461 tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg
    35521 ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc
    35581 tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc
    35641 cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa
    35701 ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg
    35761 ctacagcaac aggggagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg
    35821 gcagcgcttg aggaggttga tcaggtgatg gcagccattg ttggtgctgc tgggctgtta
    35881 cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg
    35941 gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg
    36001 gtcgatagcg aacataacgc tatttttcag agtttaccgc aacctatcca gcataatctg
    36061 ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc
    36121 cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgcccgatca agcttgccgt
    36181 catccgaact ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa
    36241 ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg
    36301 ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg
    36361 gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat
    36421 cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc
    36481 gcaccggatt atgagcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggc
    36541 caggcagcga cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg
    36601 caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat
    36661 atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gagtgaagtc
    36721 gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc
    36781 tatttgttag cgtagggctt caatgatata gtctgcgcca tctgatcgta agtagttggc
    36841 tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt
    36901 cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac
    36961 cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca
    37021 atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa
    37081 aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaagcg ttaacgctgt
    37141 atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac
    37201 tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc
    37261 gtattattgg cgataccagt cgttttaact cgcgtttgca agaacgtatt cgtaaatctg
    37321 aagcgctaac agccggaaat accggtctga cgctgaatat tgcggcgaac tacggtggac
    37381 gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc
    37441 aaccagatca gatagatgaa gagatgttaa accagcatgt ctgtatgcat gaactggccc
    37501 ctgtagattt agtaattagg actgggggag agcatcgcat tagtaacttt ttgctttggc
    37561 aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag
    37621 actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc
    37681 ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt
    37741 ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca
    37801 ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta
    37861 ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc
    37921 tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt
    37981 gggcttcgtt gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg
    38041 cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc
    38101 ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg
    38161 gcgcaatatg gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata
    38221 tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct
    38281 ggcaaggctt tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt
    38341 gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag
    38401 cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca
    38461 gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg
    38521 cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa
    38581 tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac
    38641 cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt
    38701 ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt
    38761 tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt
    38821 cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac gagtggcgat
    38881 tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt
    38941 tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca attcgatagc
    39001 tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc
    39061 tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat
    39121 tacagtagcg ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg
    39181 ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggcc
    39241 gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt
    39301 gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt
    39361 tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg
    39421 gagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg
    39481 ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca
    39541 gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct
    39601 gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg
    39661 gccgatttct atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct
    39721 gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt
    39781 acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc
    39841 cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg
    39901 gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata
    39961 acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac
    40021 ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt
    40081 ggtgcggccc tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc
    40141 agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt
    40201 gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc
    40261 tccggtaaca aatcggtgaa agatgacatg ctaaagcaaa acctcgaggc ttctggtgtg
    40321 cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac
    40381 ttctactaca gcgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg
    40441 cgcaaccgtg ttgacctaaa actggtgttc cgggaaggtg tgtcagctga aatccagcaa
    40501 attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg
    40561 cgtgacgaag tgccgtggtg gaatgtggta ggcgatcgta aataccagaa acagaaactg
    40621 gcgggcgacc ttgaaaccct gcgcagctac tatctggacc gcggttatgc ccgtttcaac
    40681 atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac
    40741 atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg
    40801 cactccgctg aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcact
    40861 aaagtgacca aaatggaaga tgacattaaa aagcttctcg gtcgctatgg ttatgcctat
    40921 ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg
    40981 aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc
    41041 tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gttggggagc
    41101 gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat
    41161 actgataccc aacgtgttcc gggtagcccg gaccaggttg atgtagtcta caaggtgaaa
    41221 gaacgtaaca ctggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc
    41281 ttccaggctg gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac
    41341 gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcacc
    41401 gtagatggcg taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc
    41461 gacctgtccg actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatt
    41521 aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg
    41581 cagcctcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacttct
    41641 gatcaggata acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac
    41701 aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg
    41761 accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg
    41821 ccgatcgatg acgatcacaa atgggttgtt ctgggtcgta cccgctgggg ttatggtgat
    41881 ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc
    41941 gtgcgtggct tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc
    42001 agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg
    42061 tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc
    42121 accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc attcttctgg
    42181 gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac
    42241 tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca
    42301 ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca
    42361 gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt
    42421 ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg
    42481 caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagctgc
    42541 aggtcttggt ttggcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa
    42601 tatgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa
    42661 tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat
    42721 gaaaaaactg cagtccatga aagcgggcag cgatcgcacc aaactggaaa aagacgtgat
    42781 ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg
    42841 ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc
    42901 caacagccag gatatcgatc tggttgttga tgcaaacgct gttgcttaca acagcagcga
    42961 tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg
    43021 actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat
    43081 caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc
    43141 aaaataccgt gagcatttag gcttgtgcca agcgtccgcg gttgtcatga cccaggacga
    43201 tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg
    43261 catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt
    43321 gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga
    43381 gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa
    43441 cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca
    43501 gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta
    43561 tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga
    43621 tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactgttat
    43681 tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga
    43741 caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg
    43801 catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt
    43861 tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat
    43921 tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga
    43981 catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat
    44041 cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat
    44101 tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg
    44161 cagattgaag agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc
    44221 gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag
    44281 ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa
    44341 gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt
    44401 gagctgtact acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc
    44461 gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa
    44521 ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc
    44581 cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa
    44641 gagggcgcgt caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat
    44701 gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa
    44761 attggccgcg ataatgagat ttatcagttt gcctccatcg gcgaagttaa ccaggatttg
    44821 aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc
    44881 gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac
    44941 ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc
    45001 gccaacaacg caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc
    45061 atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg cggctgctcc
    45121 ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc
    45181 ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc
    45241 cgcaatgcgt ataagctaat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa
    45301 attgctgaac tggcggaaac atatccggaa gtgaaagcct ttaccgattt ctttgcacgc
    45361 tcaacgcgcg gtctgattcg ttaatgactg accagcgtcc attaacgatt gccctggtcg
    45421 ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg
    45481 tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag
    45541 cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc
    45601 gtcgcttact gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg
    45661 tttttgttgg tattgatgcg cccgacttca atattactct tgaaggtaac ctcaaaaagc
    45721 agggtatcaa aaccattcat tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg
    45781 ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt
    45841 tttatgacaa atacaatgta ccgtgccgct ttatcggtca taccatggct gatgccatgc
    45901 cgttagatcc agataaaaat ggtgcccgtg atgtgctggg gatcccttac gatgcccact
    45961 gtctggcatt gttgccgggc agccgtggtg cagaagtcga aatgcttagt gccgatttct
    46021 tgaaaactgc ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccgctgg
    46081 tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagacctgt
    46141 cggttcattt gctggatgga atgggccgtg aggcgatggt cgccagcgat gcggcactac
    46201 tggcgtcggg gacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat
    46261 atcgcatgaa gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct
    46321 cgctgccaaa tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg
    46381 agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccacg
    46441 cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg
    46501 cggcacaagc cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct
    46561 ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc
    46621 ggtgatcctt gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga
    46681 aaaacgccgt ctggcgctct atgaagagat caaagagaaa gcgttgagct ggagtctggg
    46741 ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat
    46801 gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg
    46861 ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa ggggatagcc gcgtaccaga
    46921 aatcagtgcc gcgtctatcc tagcgaaagt gacgcgtgac gccgaaatgg cggcgctgga
    46981 tattgttttc ccgcaatatg gttttgccca acataaaggg tatccaaccg cttttcatct
    47041 ggaaaaactg gctgaatatg gcgcgactga acatcatcgt cgcagctttg ggcctgtcaa
    47101 acgcgcactg ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa
    47161 gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg
    47221 cttggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc
    47281 gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc
    47341 agggattaag cctatcgtcg gggcagattt taatgtccag tgcgacctgc tgggtgatga
    47401 gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc tggcgttgct
    47461 gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg
    47521 gcttatcgaa ttaaacgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg
    47581 acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga
    47641 acacttcccg gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag
    47701 ctatctgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa
    47761 cgatgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca
    47821 cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat
    47881 gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc ccagaagccc ttgccaacac
    47941 cgttgagatc gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca
    48001 gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagaaggcct
    48061 ggaagagcgt ctggcatttt tattccctga cgaggaagaa cgtcttaaac gccgcccgga
    48121 atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggtt tcccgggcta
    48181 cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtaggacc
    48241 aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga
    48301 tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc
    48361 tgacttcgac gttgacttct gtatggagaa acgcgatcag gttatcgaac acgtggcgga
    48421 catgtacggt cgtgatgcgg tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc
    48481 ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg accgtatctc
    48541 gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca
    48601 gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa
    48661 actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac
    48721 caaaattacc gattttgcgc cgctttattg cgatgaagag ggcaaacatc cggtcaccca
    48781 gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg
    48841 tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg
    48901 cgagccaccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct
    48961 gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat
    49021 caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc
    49081 tggtccgttg cagtcaggga tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga
    49141 gatctcctat ccggacgtgc agtggcagca tgaaagcctg aaatcggtac tggagccaac
    49201 ctacggcatt atcttgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta
    49261 taccctcggt ggcgcggata tgctgcgccg tgcgatgggt aagaaaaagc cggaagagat
    49321 ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact
    49381 ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca
    49441 ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc
    49501 ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct
    49561 ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct
    49621 ttaccatttc cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg
    49681 ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg
    49741 cgaactgttt gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga
    49801 aaaactgatc atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa
    49861 ctcgctgggt gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca
    49921 ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag
    49981 ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta
    50041 cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt
    50101 aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacagctg cggggctcgt
    50161 tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga
    50221 tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca
    50281 attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag
    50341 cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata
    50401 tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg
    50461 actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca
    50521 gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga
    50581 tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga
    50641 ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct
    50701 ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa
    50761 catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt
    50821 cgccgatctc ggtgcatggc agattgcgca actggcacgc catccgcagc gtccttatac
    50881 cctagattac gttcgcctgg catttgatga atttgacgaa ctggctggcg accgtgcgta
    50941 tgcagacgat aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat
    51001 cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc
    51061 agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat
    51121 gcctatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg
    51181 tggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgccttg gcgtaccggt
    51241 agtttgtacg gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga
    51301 taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc
    51361 cattctgtgg aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc
    51421 tccgcgtctg aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc
    51481 tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc
    51541 cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag
    51601 ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt
    51661 atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac
    51721 atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg
    51781 gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg
    51841 ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac
    51901 agtctcgatt tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc
    51961 atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt
    52021 tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac
    52081 gaatatcttg ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag
    52141 cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg
    52201 gttggctgtc tgttttatga ttttttcggc gggaataccc ttaaggctga tgtctctatt
    52261 tcggtcaccg agcttggttc gttgcttgac cacaccgggc cacacctgga agcggaagag
    52321 tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg
    52381 tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc
    52441 aactgccata aatcactggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg
    52501 aaaccgacgc gtaatgcgtt ggggattctg ggggggatcc cgcgccgtga atttactcgc
    52561 gacagcatcg aagagaaagt cgctgccact acgcaagcac aatggccggt tcatgcggtg
    52621 atcaccaact ccacctatga tggtttgctc tacaacaccg actggatcaa acagacgctg
    52681 gatgtcccgt cgatccactt cgattccgcc tgggtgccgt atactcattt tcatccaatc
    52741 taccagggta aaagtggtat gagcggcgag cgtgttgcgg gaaaagtttt cttcgaaacg
    52801 caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cactgatcca cattaaaggc
    52861 gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt
    52921 tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa
    52981 cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggctg
    53041 cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc
    53101 gaatgctggc ccgttgcgcc tggcgaacaa tggcacggct ttaacgatgc ggatgccgat
    53161 catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc
    53221 aatatgagtg aggaggggat cccggcggcg ctggtagcaa aattcctcga cgaacgtggg
    53281 atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat tggcatcgat
    53341 aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaagcgctc ttacgatctc
    53401 aacctgcgga tcaaaaatat gctgcccgat ctctatgctg aagatcccga tttctaccgc
    53461 aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt
    53521 tccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag
    53581 gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt
    53641 agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga
    53701 gaaatgctga ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta
    53761 gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc
    53821 gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga
    53881 gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata
    53941 ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt
    54001 tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc
    54061 ttaatgggca atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc
    54121 gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgacatc gatgcggcag
    54181 tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc
    54241 aaaaacgctt caccttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt
    54301 aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc
    54361 accatcatga cactcacgct caatagacca cttctcacct cacgccagat tctggtggcc
    54421 tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa
    54481 aatccgggcg tcactctgcg cgctattcat gtgcatcacg gtttaagtgc caatgccgat
    54541 gcctgggtta cgcattgcga aaacgtctgc caacagtggc aggtgccgct ggtggtcgaa
    54601 cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc aggcgcggca ggcgcgttat
    54661 caggcatttg cccgtaccct attgcccggc gaagtgctgg tcaccgcgca acatctcgac
    54721 gatcaatgtg aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg
    54781 gctatggcgg aagtttcgga gtttgctgga acacggctta tccgcccgtt gcttgcccgc
    54841 acgcgggggg aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa
    54901 agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg
    54961 cagcagcgtt ggccgcattt tgcggaagca acggcccgca gcgccacact ttgtgctgaa
    55021 caagagagtc tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag
    55081 gggacgctgc agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatt
    55141 cgccgctggc tggcagggca gaatgcaccg atgccttccc gcgacgcgct ggtgcgcatc
    55201 tggcaggaag tggcgctggc gcgggaagat gcttccccct gtttacgttt gggcgccttt
    55261 gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagcgaa
    55321 accattgtgc tgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaacc
    55381 gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg
    55441 cgtttcaaag cgccaggatt gctgcatatt gtcgggcgca acggcggacg taagctgaag
    55501 aaaatctggc aagagttagg cgtgccgccg tggctacgtg acaccacgcc actgctgttt
    55561 tatggagaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa
    55621 ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa
    55681 gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc
    55741 gatttcaggg tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc
    55801 ggcctcgacg accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg
    55861 caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc
    55921 aagattatcg taatcatcac aattaattgg ttgatacgta tcattcattg acataatcgc
    55981 tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt
    56041 cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc
    56101 ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca
    56161 catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata
    56221 aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg
    56281 cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc
    56341 gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc
    56401 ttctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct
    56461 ggcgtggctg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga
    56521 tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg
    56581 gctgccggat gagcgacgaa ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt
    56641 tacctataat agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca
    56701 gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt
    56761 tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggtgttatct ggttatcgga
    56821 tgataagaat aatctggaag ttaacttaac cgcctggcaa caaccttcat gattgtgatt
    56881 tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc
    56941 gctggcgggc agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg
    57001 gcgcccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc
    57061 agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc
    57121 gaagccgctc tggcccggct ggtggcagtg attaaagatt taacaaaaga acaaaaagcc
    57181 cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa
    57241 aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga
    57301 atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat
    57361 gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg ccgaactgaa
    57421 accgatgccg caaagttggc gcggcgtgct accgtgtgcc gattgcgaag gaatcgaaat
    57481 ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg
    57541 tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt
    57601 attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat
    57661 gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg aaccggcaca
    57721 atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc
    57781 ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct
    57841 ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga
    57901 aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc
    57961 cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaaacctg
    58021 cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg cctacagcat
    58081 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
    58141 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
    58201 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac
    58261 gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc
    58321 gccagtgtaa gtggatgccc ggctttggca aatgcatcag tgacaaattc cgaacaaaac
    58381 cacgactttt tgtctccttc gcacacactg cttaactgcg ctttcgccag gtcgctgacg
    58441 cactgttggc gaaaatcctc ggagaacgga ttcagtgagc acatctggcg agtcaccata
    58501 aagggaataa attcgacaat gctgcgatag ttataaccgc tatctttgat tttattggca
    58561 aacgcggtga tatctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc
    58621 ttatcactat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct
    58681 tctgcaacgt tattatcacc cagaaagatt gcaacgtgac tcacagagga agtgctgaag
    58741 acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg
    58801 agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat
    58861 ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc tggctggctg
    58921 atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc
    58981 tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc aggacccttt
    59041 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
    59101 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
    59161 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
    59221 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
    59281 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
    59341 ctggaacggc gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcaat
    59401 cgccgcagct accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt
    59461 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
    59521 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct
    59581 tggatcgcca gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc
    59641 ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
    59701 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
    59761 cggaccggct ttaaccacag cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc
    59821 aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag
    59881 cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
    59941 ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc
    60001 aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc
    60061 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
    60121 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg
    60181 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
    60241 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
    60301 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
    60361 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
    60421 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
    60481 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg
    60541 ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat
    60601 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
    60661 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
    60721 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
    60781 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca
    60841 gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact
    60901 tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca gacggcatag
    60961 gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg
    61021 cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc
    61081 tctgcggtaa aactcaccgc catctctgca gctggcgcac tttgcgcata gctggcactg
    61141 gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca
    61201 tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct
    61261 ttcagctcta ccagcgacat gccaattggg ttagggcgga aagtagagcg tgttgcgaaa
    61321 acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg ccagccgcct
    61381 tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaatgcttc caggccgcga
    61441 acggcgtcgg cctggttgta ggaagcaatg agatgcagtt cgccgttggc gctttttacc
    61501 agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt
    61561 tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg atacatacag
    61621 cctgacgata gcagcccggc gtaccgctgg tgacttcgca gctatgcagt aatacagcat
    61681 tggctttcat tttagaggca ttgatttgca tctgcttacg tgcggttgga atgctcggcg
    61741 gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg
    61801 gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt
    61861 tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg
    61921 atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac
    61981 gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa
    62041 tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa
    62101 attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcat
    62161 cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg
    62221 ttacgatcag gtttacgtat ggggagtctt tatcttcgac aaagataccg tctttcgccg
    62281 gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt
    62341 cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga ttttttgggt
    62401 tctcaacaac atcaagaact gtcggcagca ggccaacgcc atctttcagt ttgatcaagc
    62461 ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt ggcacggcaa
    62521 cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa ccagcaatcg
    62581 gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat
    62641 caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt
    62701 ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg
    62761 caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg ccgactttaa
    62821 tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg
    62881 ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt
    62941 tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact
    63001 gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttgt
    63061 agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac
    63121 ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg
    63181 gcaacgcttc cggtaacagc actttacgga cgatctgcat tggcgtggca cccattgcgc
    63241 gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc atacgggcaa
    63301 taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca atcgatgtac
    63361 cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac
    63421 ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca ataatttgcc
    63481 ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaac ccaaaaaatc
    63541 cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg
    63601 gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg
    63661 gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa tttaacgcca
    63721 ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt
    63781 tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg
    63841 cagtcagtaa atggctccgt ttgcagacgt tcctggtaat cttccgggat atccagatgc
    63901 agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt
    63961 accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc acaaatacgc
    64021 ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg gcggttaatg
    64081 tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca
    64141 tcacacagca atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc
    64201 tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac
    64261 agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag ctccagcggc
    64321 agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata
    64381 ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcaa ttcctggcca
    64441 tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta
    64501 ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg
    64561 ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc
    64621 gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtagca tctcgaatgc
    64681 ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat
    64741 caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcccatgc
    64801 cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat
    64861 ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga atttatcgac
    64921 ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc
    64981 aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac gctgaccgag
    65041 tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta ttattgcccg
    65101 catcatccgc aggggagtgt tgaagagttt cgccaggtct gcgattgccg caaaccacat
    65161 ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg
    65221 gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg
    65281 ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga ttgggtgtta
    65341 aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc gcaatgatta
    65401 aaagatgaac ggttgaaata aaaatgcatt tttccgcttg tcttcctgag ccgactccct
    65461 ataatgcgcc t
//
LOCUS       DADLZM010000006        62021 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLZM010000006 DADLZM010000000
VERSION     DADLZM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.006"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            315..401
                     /gene="leuL"
                     /locus_tag="JNY73_000400"
     CDS             315..401
                     /gene="leuL"
                     /locus_tag="JNY73_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY8046530.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            494..2065
                     /gene="leuA"
                     /locus_tag="JNY73_000401"
     CDS             494..2065
                     /gene="leuA"
                     /locus_tag="JNY73_000401"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY8046531.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2065..3156
                     /gene="leuB"
                     /locus_tag="JNY73_000402"
     CDS             2065..3156
                     /gene="leuB"
                     /locus_tag="JNY73_000402"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY8046532.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3159..4559
                     /gene="leuC"
                     /locus_tag="JNY73_000403"
     CDS             3159..4559
                     /gene="leuC"
                     /locus_tag="JNY73_000403"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY8046533.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKSGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4570..5175
                     /gene="leuD"
                     /locus_tag="JNY73_000404"
     CDS             4570..5175
                     /gene="leuD"
                     /locus_tag="JNY73_000404"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY8046534.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5224..6402)
                     /gene="setA"
                     /locus_tag="JNY73_000405"
     CDS             complement(5224..6402)
                     /gene="setA"
                     /locus_tag="JNY73_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY8046535.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6504..6635)
                     /gene="sgrT"
                     /locus_tag="JNY73_000406"
     CDS             complement(6504..6635)
                     /gene="sgrT"
                     /locus_tag="JNY73_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY8046536.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6724..8379
                     /locus_tag="JNY73_000407"
     CDS             6724..8379
                     /locus_tag="JNY73_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY8046537.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8413..8512
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8543..9526
                     /gene="thiB"
                     /locus_tag="JNY73_000408"
     CDS             8543..9526
                     /gene="thiB"
                     /locus_tag="JNY73_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY8046538.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9502..11112
                     /gene="thiP"
                     /locus_tag="JNY73_000409"
     CDS             9502..11112
                     /gene="thiP"
                     /locus_tag="JNY73_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY8046539.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11096..11794
                     /gene="thiQ"
                     /locus_tag="JNY73_000410"
     CDS             11096..11794
                     /gene="thiQ"
                     /locus_tag="JNY73_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY8046540.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIVWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11908..12672)
                     /locus_tag="JNY73_000411"
     CDS             complement(11908..12672)
                     /locus_tag="JNY73_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8046541.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12758..13636)
                     /gene="araC"
                     /locus_tag="JNY73_000412"
     CDS             complement(12758..13636)
                     /gene="araC"
                     /locus_tag="JNY73_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY8046542.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13975..15674
                     /gene="araB"
                     /locus_tag="JNY73_000413"
                     /pseudo
     CDS             13975..15674
                     /gene="araB"
                     /locus_tag="JNY73_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            15685..17187
                     /gene="araA"
                     /locus_tag="JNY73_000414"
     CDS             15685..17187
                     /gene="araA"
                     /locus_tag="JNY73_000414"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY8046543.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17387..18082
                     /gene="araD"
                     /locus_tag="JNY73_000415"
     CDS             17387..18082
                     /gene="araD"
                     /locus_tag="JNY73_000415"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY8046544.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSDVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18157..20508
                     /gene="polB"
                     /locus_tag="JNY73_000416"
     CDS             18157..20508
                     /gene="polB"
                     /locus_tag="JNY73_000416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY8046545.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20673..23579
                     /gene="rapA"
                     /locus_tag="JNY73_000417"
     CDS             20673..23579
                     /gene="rapA"
                     /locus_tag="JNY73_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY8046546.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23591..24250
                     /gene="rluA"
                     /locus_tag="JNY73_000418"
     CDS             23591..24250
                     /gene="rluA"
                     /locus_tag="JNY73_000418"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY8046547.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24367..25182)
                     /gene="djlA"
                     /locus_tag="JNY73_000419"
     CDS             complement(24367..25182)
                     /gene="djlA"
                     /locus_tag="JNY73_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY8046548.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25437..27791
                     /gene="lptD"
                     /locus_tag="JNY73_000420"
     CDS             25437..27791
                     /gene="lptD"
                     /locus_tag="JNY73_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY8046549.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTYVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27844..29130
                     /gene="surA"
                     /locus_tag="JNY73_000421"
     CDS             27844..29130
                     /gene="surA"
                     /locus_tag="JNY73_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY8046550.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKLSEEAASWMQEQRASAYVKILSN"
     gene            29130..30119
                     /gene="pdxA"
                     /locus_tag="JNY73_000422"
     CDS             29130..30119
                     /gene="pdxA"
                     /locus_tag="JNY73_000422"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY8046551.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGITEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30116..30937
                     /gene="rsmA"
                     /locus_tag="JNY73_000423"
     CDS             30116..30937
                     /gene="rsmA"
                     /locus_tag="JNY73_000423"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY8046552.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30940..31317
                     /gene="apaG"
                     /locus_tag="JNY73_000424"
     CDS             30940..31317
                     /gene="apaG"
                     /locus_tag="JNY73_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY8046553.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31324..32166
                     /gene="apaH"
                     /locus_tag="JNY73_000425"
     CDS             31324..32166
                     /gene="apaH"
                     /locus_tag="JNY73_000425"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY8046554.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32244..32723)
                     /gene="folA"
                     /locus_tag="JNY73_000426"
     CDS             complement(32244..32723)
                     /gene="folA"
                     /locus_tag="JNY73_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY8046555.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32914..34776)
                     /gene="kefC"
                     /locus_tag="JNY73_000427"
     CDS             complement(32914..34776)
                     /gene="kefC"
                     /locus_tag="JNY73_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY8046556.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34769..35299)
                     /gene="kefF"
                     /locus_tag="JNY73_000428"
     CDS             complement(34769..35299)
                     /gene="kefF"
                     /locus_tag="JNY73_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY8046557.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35347..36741)
                     /locus_tag="JNY73_000429"
     CDS             complement(35347..36741)
                     /locus_tag="JNY73_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8046558.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36800..37087)
                     /gene="fixX"
                     /locus_tag="JNY73_000430"
     CDS             complement(36800..37087)
                     /gene="fixX"
                     /locus_tag="JNY73_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY8046559.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37084..38369)
                     /locus_tag="JNY73_000431"
                     /pseudo
     CDS             complement(37084..38369)
                     /locus_tag="JNY73_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(38420..39361)
                     /locus_tag="JNY73_000432"
     CDS             complement(38420..39361)
                     /locus_tag="JNY73_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY8046560.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQTRQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVRISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39376..40146)
                     /locus_tag="JNY73_000433"
     CDS             complement(39376..40146)
                     /locus_tag="JNY73_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY8046561.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40617..42131
                     /locus_tag="JNY73_000434"
     CDS             40617..42131
                     /locus_tag="JNY73_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HAY8046562.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42162..43304
                     /gene="caiA"
                     /locus_tag="JNY73_000435"
     CDS             42162..43304
                     /gene="caiA"
                     /locus_tag="JNY73_000435"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY8046563.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43433..44650
                     /gene="caiB"
                     /locus_tag="JNY73_000436"
     CDS             43433..44650
                     /gene="caiB"
                     /locus_tag="JNY73_000436"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY8046564.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44724..46277
                     /gene="caiC"
                     /locus_tag="JNY73_000437"
     CDS             44724..46277
                     /gene="caiC"
                     /locus_tag="JNY73_000437"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY8046565.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46386..47171
                     /gene="caiD"
                     /locus_tag="JNY73_000438"
     CDS             46386..47171
                     /gene="caiD"
                     /locus_tag="JNY73_000438"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY8046566.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47177..47767
                     /gene="caiE"
                     /locus_tag="JNY73_000439"
     CDS             47177..47767
                     /gene="caiE"
                     /locus_tag="JNY73_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY8046567.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47853..48248)
                     /gene="caiF"
                     /locus_tag="JNY73_000440"
     CDS             complement(47853..48248)
                     /gene="caiF"
                     /locus_tag="JNY73_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY8046568.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48506..51727)
                     /gene="carB"
                     /locus_tag="JNY73_000441"
     CDS             complement(48506..51727)
                     /gene="carB"
                     /locus_tag="JNY73_000441"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY8046569.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51745..52893)
                     /gene="carA"
                     /locus_tag="JNY73_000442"
     CDS             complement(51745..52893)
                     /gene="carA"
                     /locus_tag="JNY73_000442"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY8046570.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(53349..54170)
                     /gene="dapB"
                     /locus_tag="JNY73_000443"
     CDS             complement(53349..54170)
                     /gene="dapB"
                     /locus_tag="JNY73_000443"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY8046571.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(54337..55251)
                     /gene="rihC"
                     /locus_tag="JNY73_000444"
     CDS             complement(54337..55251)
                     /gene="rihC"
                     /locus_tag="JNY73_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY8046572.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(55317..56267)
                     /gene="ispH"
                     /locus_tag="JNY73_000445"
                     /gene_synonym="lytB"
     CDS             complement(55317..56267)
                     /gene="ispH"
                     /locus_tag="JNY73_000445"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY8046573.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(56269..56718)
                     /gene="fkpB"
                     /locus_tag="JNY73_000446"
     CDS             complement(56269..56718)
                     /gene="fkpB"
                     /locus_tag="JNY73_000446"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8046574.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(56805..57299)
                     /gene="lspA"
                     /locus_tag="JNY73_000447"
     CDS             complement(56805..57299)
                     /gene="lspA"
                     /locus_tag="JNY73_000447"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY8046575.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(57299..60115)
                     /gene="ileS"
                     /locus_tag="JNY73_000448"
     CDS             complement(57299..60115)
                     /gene="ileS"
                     /locus_tag="JNY73_000448"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY8046576.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(60158..61099)
                     /gene="ribF"
                     /locus_tag="JNY73_000449"
     CDS             complement(60158..61099)
                     /gene="ribF"
                     /locus_tag="JNY73_000449"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY8046577.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(61107..61325)
                     /locus_tag="JNY73_000450"
     CDS             complement(61107..61325)
                     /locus_tag="JNY73_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY8046578.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            61428..61691
                     /gene="rpsT"
                     /locus_tag="JNY73_000451"
     CDS             61428..61691
                     /gene="rpsT"
                     /locus_tag="JNY73_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY8046579.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        14917 a        16933 c        16134 g        14037 t
ORIGIN      
        1 tcaatatcaa aaaaaaatcg caaaacatat aattcaatac aaatcatcag gataggtttt
       61 gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat ttatgctgaa
      121 atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc ttactttaac
      181 caccgtagca caattagcta attttacgga tgcagaactc acgctggcgg gacgttttta
      241 ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata tcgcattcat
      301 ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta ctaaacgcat
      361 cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg cagtcaaaca
      421 aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc taaaagagac
      481 aaggacccaa accatgagcc agcaagtcat tattttcgat accacattgc gcgacggtga
      541 acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc tggcccttga
      601 gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg gcgattttga
      661 atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg
      721 cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag ccttccgtat
      781 tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca gcaccctgga
      841 cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt
      901 tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc gagtggtcga
      961 agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg gctacaccat
     1021 gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa
     1081 agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca actcactggc
     1141 ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc
     1201 cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg atattctcaa
     1261 cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag ttagccagat
     1321 ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcggcgcat tcgctcactc
     1381 ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc
     1441 agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc
     1501 ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt tagacaattt
     1561 gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt acgatctgga
     1621 ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc tggattactt
     1681 cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg cctgtggcga
     1741 agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct atcaggcaat
     1801 taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca
     1861 cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc gccgcttcca
     1921 cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg tgcacgtact
     1981 gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag ctcaacacaa
     2041 cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt attgccggga
     2101 gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc cgtgcgcaac
     2161 cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc cattgataac
     2221 cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg
     2281 tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca accagaacgc
     2341 ggcgcgctgc tgccgctgcg taagcacttc aaattattca gcaacctgcg cccggcaaaa
     2401 ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc taacggcttc
     2461 gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc aaaaggccgc
     2521 gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg ttttgagatc
     2581 gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa agtcacctca
     2641 atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt caacgagatc
     2701 gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc caccatgcag
     2761 ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt
     2821 ctgtctgacg agtgcgcaat gatcactggc tcgatgggca tgttgccttc cgccagcctg
     2881 aacgagcaag gttttggact gtatgaaccg gcgggcggct cggcaccaga tatcgcaggc
     2941 aaaaacatcg ccaacccgat tgcacaaatc ctgtcgctgg cactgctgct gcgttacagc
     3001 ctggatgccg atgatgcggc ttccgccatt gaacgcgcca ttaaccgtgc attagaagaa
     3061 ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga tgaaatgggc
     3121 gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa
     3181 aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg
     3241 accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg
     3301 gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac gtctctaccc
     3361 agaccaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta
     3421 aaaactgcaa agaatttggc gtcgagctgt atgacctgaa tcacccgtat caggggatcg
     3481 tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt gtctgcggcg
     3541 actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg
     3601 aagttgaaca cgtactggca acacaaaccc tgaaacaggg ccgtgcgaag accatgaaaa
     3661 ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta
     3721 tcggtaaaac tggcagcgca ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa
     3781 ttcgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcgatc gaaatgggcg
     3841 caaaatccgg tctggttgct cctgacgaaa ccacctttaa ctatgtcaaa ggccgtctgc
     3901 atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg
     3961 acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt tcaccgcagg
     4021 tcacctgggg caccaatccc ggccaggtga tttccgtgaa cgacaatatt cccgatccgg
     4081 cttcgtttgc cgatccggtt gaacgtgcgt cggcagaaaa agcgctggcc tatatggggc
     4141 tgaaaccggg tattccgctg accgaagtgg ctatcgacaa agtgtttatc ggttcctgta
     4201 ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg
     4261 cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc caggcggaag
     4321 cggaaggtct ggataaaata tttattgaag ccggttttga atggcgcttg cctggctgct
     4381 caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt gcatccacca
     4441 gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg
     4501 caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac attaaataag
     4561 gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc gctggatgcc
     4621 gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt gacccgtacg
     4681 ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg ccaacagcca
     4741 aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt gctggcacga
     4801 gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac cgactacggt
     4861 tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag ctttaacaac
     4921 cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc gctggtgaaa
     4981 gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa agcgggagag
     5041 aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa tggtctggac
     5101 agcatcggcc tgaccctgca gcacgacgac gcgattgcgt cttatgaagc aaaacaacct
     5161 gcgtttatga attagccccc ttgcccggtc aaatgaccgg gctttccgct atcgtccacg
     5221 tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa taatcgcaat
     5281 tacccagtag acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacaccagc
     5341 cagaattacc ccggtagaga tagtgttggt aaataaggtg gtagctgccc ccgctcttcc
     5401 aggcattaaa tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc
     5461 gttaaaaagt tgcagcgtca tcaacgccat acggctatga aagaaaatca atccagtgta
     5521 aaacagcact cctgccacca ctgcaatgac catcattcgc cgcttaccat aacgtttgac
     5581 atagtagcca gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa
     5641 acccgccagt ttgtctggca atcctaaatc gctactgatc cacaacggca tatcaataat
     5701 gtacatagtg ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact
     5761 atcctgccag tcaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac
     5821 agacggaagc ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga
     5881 aaacatcact gtaaagccgt aattcaacgc cagcataaag gccagcggtg gaccgattac
     5941 ccatgccaga gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga
     6001 gttatccgcg tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga
     6061 tgccagaagc acaccacagg tgataagcgt cagataatga cgattaaatg caaacagtag
     6121 cgcattgcca atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact
     6181 gtcagaacgt tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata
     6241 aaagagtccg atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt
     6301 aggagcctgt agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta
     6361 aacaccgttc atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc
     6421 ggcgagaata aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact
     6481 cagtcacaca tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt
     6541 ccttcaagca tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct
     6601 gtcgcggtaa aatagtgctg ataaaactga cgcatggggc atctccttgc ttcatcgtta
     6661 ggaattattg acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct
     6721 tttatgccat ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt
     6781 aaatcgcagg acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat
     6841 atgcgtaccc tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc
     6901 gggcgcggta aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag
     6961 cgggcggaag acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac
     7021 aaagcgactg tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg
     7081 cacatcctgc gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg
     7141 cgccgttccg aaacccatat tgcccggcaa atcttcagtt cgctaacgcg cataaatgag
     7201 gaaaatgggg aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac
     7261 tggcgtttct ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac
     7321 gacgtgatcg cctctttaaa acgaatcaat acgctgccgc tctattcgca tattacggac
     7381 attgtgtcgc cgacgccctg gacgctggat atccatctca cgcagccgga ccgctggttg
     7441 ccgttactgc tggggcaagt tccggcgatg atcctgccgt gcgaatggga aaccctcagt
     7501 aactttgcca gccatcctat tggcaccggt ccgtatgcgg tgattcgcaa cagcaccaat
     7561 caactgaaaa ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgacgaagtt
     7621 aacgtctggg ttctgccgga aattgccgac gagccagccg gtgatctgat gctaaaaggt
     7681 ccacagggcg aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttactg
     7741 ttcgacagcc gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg
     7801 ctttctccaa ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg
     7861 gcttatggac tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct
     7921 ggcctggaaa gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc
     7981 gggatcatgc agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc
     8041 tacgatcagt ggcatgaagg agagatcgag agcgatatct ggcttaatag cgccaacttt
     8101 acgctgccgc tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac
     8161 tgtattccga tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg
     8221 gcgaactggt gccagcaact ggtcgccagt aaagcaatgg tgccgcttat ccaccactgg
     8281 ctgatcattc agggacaacg cagtatgcgc ggcctgcgta tgaataccct cggctggttc
     8341 gattttaaat cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc
     8401 actacactaa cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc
     8461 gtcgaacctg atccggataa cgccggcgaa gggatttgag gctccttctc aagtcctttg
     8521 ccactctttt ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac
     8581 agcgcccgtt ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga
     8641 ctgggggcct ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa
     8701 actggtggcg ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa
     8761 tagtaaagcc gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac
     8821 cggactattt gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa
     8881 taatgacact ttcgtcccgt ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa
     8941 actgaaaaac ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt
     9001 gatttatcag gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa
     9061 agtctatggc gatgacgccc cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt
     9121 caccaaaggc tggagcgaag cctacggcct gtttttaaaa ggtgaaagcg atctggtact
     9181 gagttacacc acctctccgg cttatcacat tctcgaagag aaaaaagata actacgccgc
     9241 cgcgaacttt agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag
     9301 caagcagccg gagctggcgc aaaaattcct ccagtttatg gtttctccgg cttttcagaa
     9361 tgcgatccca accggtaact ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt
     9421 tgaacaattg accaaaccgg ccaccacgct ggagttcaca ccagctgaag tggcggcaca
     9481 acgtcaggca tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta
     9541 attccaggtg taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc
     9601 ctgtggtgga acgcgccgca gggtaactgg gtggcagtct ggcaggacag ctacctgtgg
     9661 catgtggtgc gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc
     9721 gcgatattcc tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg
     9781 cgtctgtgcg caatgacctt gatcctcccg gtgctagttg ctgttttcgg cattcttagc
     9841 gtctatggtc gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc
     9901 ttttcgccct acggcctgca aggcattttg ctggcgcacg tattttttaa tctgccgatg
     9961 gcgagccgct tattactcca ggcactggaa aacattcccg gcgaacaacg tcagcttgcc
    10021 gcccagcttg ggatgcgcgg ctggcatttt ttccgcttcg tcgaatggcc gtggctacgg
    10081 cgacaaatcc cgccggttgc cgcgcttatc tttatgctct gtttcgccag cttcgccacc
    10141 gtgctatcgc tgggcggcgg tccgcaggca accactatcg agctggctat ttatcaggcg
    10201 ctgagttacg actacgatcc tgcccgcgcg gcgatgctgg cgctgctcca gatggtgtgt
    10261 tgccttgggc tggtgctgtt gagtcagcga ctaagtaagg ccattgcgcc cggcaccacg
    10321 ctgctgcaag gctggcgcga tccggacgat cgtctgcata gccgcatttg cgacacggtg
    10381 ttaatagtgc tggcgctgct gctgttgctg ccaccgttgc tggcggtgat cgtcgatggg
    10441 gtaaatcgcc agttgccgga agtgctggca caaccggtgc tgtggcaggc gctgtggacc
    10501 tcgttgcgta ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta
    10561 tggagcagtc gcgaactgcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg
    10621 agcggcatgt tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg
    10681 ctcaacaaca ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg
    10741 ttaatggcga tcccttatgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc
    10801 cgctacagta tgttgtgtca gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc
    10861 gagctgcgcg ccctgaaacg tccactggcg caggcgctgg cttttgcctg cgtgctgtcg
    10921 attggtgatt ttggcgtagt ggcgttgttc ggtaacgatg atttccgtac cctgccgttt
    10981 tatctctacc agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt
    11041 ctgctactac tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt
    11101 aaaactgact gatatcacct ggctttatca ccatttgccg atgcgtttta gcttaacggt
    11161 agaacgcggc gagcaggtgg caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct
    11221 gaatttgatt gccggttttc tgacgccagc cagcggttcg ctgactatcg atggcgttga
    11281 tcacaccacg acgccgccgt cacgccgtcc ggtgtcgatg ctgtttcagg agaacaacct
    11341 gttcagccac ctgacggtcg cgcagaacat cgggctgggg ctaaatccgg ggttgaaact
    11401 gaacgcggca cagcaggaga aaatgcacgc gattgcccgt cagatgggga ttgataattt
    11461 aatggcgcgg ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg
    11521 ttgtctggtg cgcgaacagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc
    11581 ggcgttacgt caggagatgc tgacgctggt gagcacgagc tgccagcagc aaaaaatgac
    11641 gctattgatg gtgtcgcaca gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt
    11701 ggtcgccgac gggcgcatcg tctggcaggg taagactgaa gaattgttga gcggtaaggc
    11761 gagtgcttcg gcaatattgg ggattacggg ttagtatgca ctgtcagatg cggcgtgaac
    11821 accttatccg acctacggac agtgcgtttt gtaggcctga taagacgcgg tcagcgtcgc
    11881 atgaggcaac ccgcacaaga caatccccta aaccccaacc actttacgca aaatatcgat
    11941 atacaccggc atcaacgggt ggcgaattaa caccaccagc gccaccacgc cgatggcaga
    12001 aatcaacggc gtcagccaca acaaacggcc gcgcgacaaa taatgactta aacgatcggt
    12061 tgctttaccg ctgcgccata accgccagca cagccagcca ccaacccaca aaaataccgc
    12121 cgtcgccagc agcaaccatt taaactcgcc gctctgcatt ccggctggaa tatcgatcgc
    12181 cgcgcccgcc agaatcccag gcaggaagta aaacggtggc cacagcaggc agccgataat
    12241 attcggcgta atgaattttg ccaccggcag atccagcatt cccgccacca ttggcaccag
    12301 cggacgcgtc gggccaacaa agcgaccgac cagaatggtg aacatgctgt gttgatgcaa
    12361 cgcatgttca gttttatcga gtaatgcttt gtttttcttc agaaatgacc agcgatgcaa
    12421 cggcttttta aaacgccagc ccagccagaa ggagatccag tcgcccagca gacaaccgac
    12481 aatccctgcc agccaagcgt gccagaaact taactcgccg ctgccaatca gcgctcccag
    12541 ccccgccatc agcaccgtac cgggtagaat caaaccgacc agcgccagcg actccagaaa
    12601 ggccaccaac accaccgcca tcaatgaata cacggtagat tgggtaataa agtgttccag
    12661 caatgcttgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
    12721 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
    12781 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
    12841 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
    12901 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
    12961 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
    13021 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
    13081 tgtactgaca agcctcgcgt acacgattat ccatcggtgg atggagcgac tcgttaatcg
    13141 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
    13201 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tacggctggt
    13261 gcgcttcatc cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt
    13321 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
    13381 gatgacaacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
    13441 agacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
    13501 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
    13561 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
    13621 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
    13681 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta acccaaccgg
    13741 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
    13801 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
    13861 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
    13921 tatcgcaact ctctactgtt tctccatacc cgtttttttg gatggagtga aacgatggcg
    13981 attgcaattg gcctcgattt tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc
    14041 agcggtgaag agatcgccac cagcgtagag tggtatcccc gttggcaaaa agggcaattt
    14101 tgtgatgccc cgaataacca gttccgtcat catccgcgtg actacattga gtcaatggaa
    14161 gcggcactga aaaccgtgct tgcagagctt agcgtcgaac agcgcgcagc tgtggtcggg
    14221 attggcgttg acagtaccgg ctcgacgccc gcaccgattg atgccgacgg taacgtgctg
    14281 gcgctgcgcc cggagtttgc cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac
    14341 actgcggttg aagaagcgga agagattacc cgtttgtgcc acgcgccggg caacgttgac
    14401 tactcccgct atattggcgg tatttattcc agcgaaggtt ctgggcaaaa atcctgcatg
    14461 tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact
    14521 gggtgccagc tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc ggacgttgca
    14581 gcgccgggca taaatctctg tggcacgaaa gctggggcgg cttgccgcca gccagtttct
    14641 ttgatgagct ggacccgatc ctcaatcgcc atttgccttc cccgctgttc actgacacct
    14701 ggactgccga tattccggtg ggcaccttat gcccggaatg ggcgcagcgt ctcggcctgc
    14761 ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca gttggcgcag
    14821 gcgcacagcc taacgcactg gtaaaagtta tcggtacttc cacctgcgac attctgattg
    14881 ccgacaaaca gagcgttggc gagcgggcag ttaaaggtat ttgcggtcag gttgatggca
    14941 gcgtggtgcc tggatttatc ggtctggaag caggccaatc ggcgtttggt gatatctacg
    15001 cctggtttgg tcgcgtactc ggctggccgc tggaacagct tgccgcccag catgcggaac
    15061 tgaaagcgca aatcaacgcc agccagaaac aactgcttcc ggcgctgacc gaagcatggg
    15121 ccaaaaatcc gtctctggat cacctgccgg tggtgctcga ctggtttaac ggtcgtcgca
    15181 cgccaaacgc taaccaacgc ctgaaagggg tgattaccga tcttaacctc gctaccgaca
    15241 ctccgctgct gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc gcaatcatgg
    15301 agtgctttac cgatcagggg atcgccgtca ataacgtgat ggcgctgggc ggcatcgcgc
    15361 ggaaaaacca ggtcattatg caggcctgct gcgacgtgct gaatcgcccg ctgcaaattg
    15421 ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt tgctgccgtc gccgcgaaag
    15481 tgcacgcaga catcccatca gcccagcaaa aaatggccag tgcggtagag aaaaccctgc
    15541 aaccgcgcag cgaacaggca caacgctttg aacagcttta tcgccgctat cagcaatggg
    15601 cgatgagcgc cgaacaacac tatcttccaa cttccgcccc ggcacaggct gcccaggccg
    15661 ttgcgactct ataaggacac gataatgacg atttttgata attatgaagt gtggtttgtc
    15721 attggcagcc agcatctgta tggcccggaa accctgcgtc aggtcaccca acatgccgag
    15781 cacgttgtta atgcgctgaa tacggaagcg aaactgccct gcaaactggt gttgaaaccg
    15841 ctgggcacca cgccggatga aatcaccgct atttgccgcg acgcgaatta cgacgatcgt
    15901 tgcgctggtc tggtggtgtg gctgcacacc ttctccccgg ccaaaatgtg gatcaacggc
    15961 ctgaccatgc tcaacaaacc gttgctgcaa ttccacaccc agttcaacgc ggcgctgccg
    16021 tgggacagta tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg cggtcgcgag
    16081 ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg tcgttaccgg tcactggcag
    16141 gataaacaag cccatgagcg tatcggctcc tggatgcgtc aggcggtctc taaacaggat
    16201 acccgtcatc tgaaagtctg ccgatttggc gataacatgc gtgaagtggc ggtcaccgat
    16261 ggcgataaag ttgccgcaca gatcaagttc ggtttctccg tcaatacctg ggcggttggc
    16321 gatctggtgc aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct ggtcgatgag
    16381 tacgaaagct gctacaccat gacgcctgcc acacaaatcc acggcgaaaa acgacagaac
    16441 gtgctggaag cggcgcgtat tgagctgggg atgaagcgtt tcctggaaca aggtggcttc
    16501 cacgcgttca ccaccacctt tgaagatttg cacggtctga aacagcttcc aggtctgccc
    16561 gtacagcgtc tgatgcagca gggttacggc tttgcgggcg aaggcgactg gaaaaccgcc
    16621 gccctgcttc gcatcatgaa ggtgatgtca accggtctgc agggcggcac ctcctttatg
    16681 gaggactaca cctatcactt cgagaaaggt aatgacctgg tgctcggctc ccatatgctg
    16741 gaagtctgcc cgtcgattgc cgtagaagag aaaccgatcc tcgacgttca gcatctcggt
    16801 attggtggta aggacgatcc tgcccgactg atcttcaata cccaaaccgg tccagcgatt
    16861 gtcgccagcc tgattgatct cggcgatcgt taccgtctgc tggttaactg tatcgacacg
    16921 gtgaaaacac cgcactccct gccgaaactg ccggtggcga atgcactgtg gaaagcgcaa
    16981 ccggatctgc caactgcttc cgaagcgtgg atcctcgctg gtggcgcgca ccataccgtc
    17041 ttcagccatg cgctgaacct caacgatatg cgccagttcg ccgagatgca cgacattgaa
    17101 atcacggtga ttgataacga cacccgcctg ccagcgttta aagacgcact gcgctggaac
    17161 gaagtgtatt acggatttcg tcgctaagta gccacatcag gtatgtaacg cctgatgcga
    17221 cgctgactcg tcttatctgg cctacacgct gcgattttgt aggccggata agcaaagcgc
    17281 atccggcatt caacgcctga tgcgacgctg gcgcgtctta tcaggcctac gcgctgcgat
    17341 tttgtagacc ggataagcga agcgcatccg gcacgaagga gtcaacatgt tagaagatct
    17401 caaacgccag gtattagaag ccaacctggc gctgccaaaa cacaacctgg tcacgctcac
    17461 atggggcaac gtcagcgacg ttgatcgcga gcgcggcgtc tttgtgatca aaccttccgg
    17521 cgtcgattac agcgtcatga ccgctgacga tatggtcgtg gttagcatcg aaaccggtga
    17581 agtggttgaa ggtacgaaaa agccctcctc cgacacgcca actcaccggc tgctctatca
    17641 ggcattcccc tccattggcg gcattgtgca tacgcactcg cgccacgcca ccatctgggc
    17701 gcaggcgggt cagtcgattc cagcaaccgg caccacccac gccgactatt tctacggcac
    17761 cattccctgc acccgcaaaa tgaccgacgc agaaatcaac ggcgaatatg agtgggaaac
    17821 cggtaacgtc atcgtagaaa cctttgaaaa acagggtatc gatgcagcgc aaatgcccgg
    17881 cgttctggtc cattcccacg gcccgtttgc atggggcaaa aatgccgaag atgcggtgca
    17941 taacgccatc gtgctggaag aggtcgctta tatggggata ttctgccgtc agttagcgcc
    18001 gcagttaccg gatatgcagc aaacgctgct ggataaacac tatctgcgta agcatggcgc
    18061 gaaggcatat tacgggcagt aatgactgta taaaaccaca gccaatcaaa cgaaaccagg
    18121 ctatactcaa gcctggtttt ttgatggatt ttcagcgtgg cgcaggcagg ttttatctta
    18181 acccgacact ggcgggacac cccgcaaggg acagaagtct ccttctggct ggcgacggac
    18241 aacgggccgt tgcaggttac gcttgcaccg caagagtccg tggcgtttat tcacgccgat
    18301 caggttcccc gcgctcagca tattttgcag ggtgaacaag gctttcgtct gacaccgctg
    18361 gcgttaaagg attttcaccg ccagccggtg tatggccttt actgtcgcgc ccatcgccaa
    18421 ttgatgaatt acgaaaagcg cctgcgtgaa ggtggcgtta ccgtctacga ggccgatgtg
    18481 cgcccgccag aacgctatct gatggagcgg tttatcacct caccggtgtg ggtcgaaggt
    18541 gatatgcaca atggcactat cgttaatgcc cgtctgaaac cgcatcccga ctatcgtccg
    18601 ccgctcaagt gggtttctat agatattgaa accacccgcc acggtgagct gtactgcatc
    18661 ggcctggaag gctgcgggca gcgcatcgtt tatatgctgg ggccggagaa tggcgacgcc
    18721 tcctcgcttg atttcgaact ggaatacgtc gccagccgcc cgctgctgct ggaaaaactc
    18781 aacgcctggt ttgcaaacca cgatcccgat gtgatcatcg gctggaacgt ggtgcagttc
    18841 gatctgcgaa tgctgcaaaa acatgccgag cgttaccgtc ttccgctgcg tcttgggcgc
    18901 gataatagcg agctggagtg gcgcgagcac ggctttaaaa acggcgtctt ttttgcccag
    18961 gctaaaggtc ggctaattat cgacggtatc gaggcgctga aatccgcgtt ctggaatttc
    19021 tcttcattct cgctggaaac tgtcgctcag gagctattag gcgaaggaaa atctatcgat
    19081 aacccgtggg ttcgaatgga cgaaattgac cgccgtttcg ccgaagataa acctgcgctg
    19141 gcaacttata acctgaaaga ttgcgagctg gtgacgcaga tcttccacaa aactgaaatc
    19201 atgccatttt tactcgaacg agcaacggtg aacggcctgc cggtggatcg gcacggcggt
    19261 tcggtggcag cgtttggtca tctctatttt ccgcgaatgc atcgcgctgg ttatgtcgcg
    19321 cctaatctcg gcgaagtgcc gccgcacgcc agccctggcg gctacgtgat ggattcacgg
    19381 ccagggcttt atgattcagt gctggtgctg gactataaaa gcctgtaccc gtcgatcatc
    19441 cgcacctttc tgattgatcc cgtcgggctg gttgaaggca tggcgcaacc tgatccagag
    19501 cacagtaccg aaggttttct cgatgcctgg ttctcacggg agaaacattg cctgccggag
    19561 attgtgacta acatctggca cgggcgcgat gaagccaaac gtcagggtaa taagccgctt
    19621 tcgcaggcgc tgaagatcat tatgaatgcc ttttatggcg tgctcggcac caccgcctgc
    19681 cgcttcttcg atccgcgcct ggcatcgtcg atcaccatgc gtggtcatca gatcatgcgg
    19741 cagaccaaag cattgattga agcgcagggc tacgacgtta tctacggaga taccgactcg
    19801 acttttgtct ggctaaaagg tgcacattcg gaagaagagg cggcgaaaat cggtcgtgca
    19861 ctggtgcagc acgttaacgc ctggtgggcg gaaacgctgc aaaaacaacg gctgaccagc
    19921 gcattagaac tggagtatga aactcatttc tgccgttttc ttatgccaac cattcgcgga
    19981 gctgataccg gcagtaaaaa gcgctacgcc gggctgattc aggagggcga caagcagcgg
    20041 atggtgttta aagggctgga aaccgtgcgc accgactgga cgccgctggc ccagcagttt
    20101 cagcaggagc tgtacctgcg tatcttccgc aacgagccat atcaggagta cgtccgccaa
    20161 accatcgaca aactgatggc gggtgaactg gatgcgcggc tggtttaccg taaacgcctt
    20221 cgccgtccgc tgagcgaata tcagcgtaat gttccgcctc atgtacgcgc cgctcgcctt
    20281 gccgatgaag aaaatcaaaa gcgcggtcgc cccttgcaat atcagaatcg cggcaccatt
    20341 aagtacgtat ggaccaccaa cggcccggag ccgctggact accaacgttc accactggat
    20401 tacgaacact atctgacccg ccagctacaa cccgtggcgg agggaatact cccttttatt
    20461 gaggataatt ttgctacact tatgaccggg caacttgggc tattttgagc aaaaaaaaga
    20521 gttcgccaga taccattttg atgcgtgacg aatgctttgc catccagtac catagcgccc
    20581 tttccattcc tggacctgaa taacaccact acctcataag cacggtagcg ggtggttatt
    20641 gcctgcaatt aaagatatag agccgaacac atatgccttt tacacttggt caacgctgga
    20701 tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat gcgcgaactg
    20761 tcactttact tttcccatct actggtgaaa accgtctgta cgcacgcagt gattcccccg
    20821 tgacccgcgt gatgttcaac cctggtgata ccattaccag ccatgacggc tggcagatgc
    20881 aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta tatcggtact cgcctggata
    20941 ctgaagagtc cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg gtgttcagca
    21001 aaccgcagga ccgtctgttt gccgggcaga ttgaccgtat ggaccgcttt gcgctgcgtt
    21061 atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc gtacagcggc ctgcgcggtc
    21121 agcgtaccag cctgatcccg catcagctca acatcgctca tgatgttggt cgccgccacg
    21181 cgccgcgcgt cctgctggct gacgaagtgg gtttagggaa aaccattgaa gccgggatga
    21241 tcctgcatca gcaactgctc tctggcgctg ctgaacgtgt gctgattatc gtcccggaaa
    21301 ccttacagca tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc tttgctctgt
    21361 ttgatgatga gcgttatgcc gaagctcagc acgatgctta caacccgttt gacaccgaac
    21421 agctggtgat ttgctcgctg gattttgccc gtcgtagcaa acagcgcctg gaacatctct
    21481 gtgaagccga atgggacctg ctggtggtcg atgaagcgca tcacctggtg tggagcgaag
    21541 atgcgccaag ccgtgaatat caggccattg aacaactggc agagcacgtg ccgggcgttc
    21601 tgctgctgac cgcgaccccg gaacagctgg ggatggaaag ccacttcgcc cgtctgcgtc
    21661 tgctggaccc gaaccgtttc cacgattttg cgcagttcgt tgaagagcag aaaaattatc
    21721 gtccggttgc ggacgccgtt gccatgctgc tggcaggtaa caaactgagc aatgacgaac
    21781 tgaacatgct cggcgagatg atcggcgagc aggatatcga gccgctgttg caggcagcaa
    21841 acagcgacag cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg ctgatggatc
    21901 gccacggcac cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa ggattcccga
    21961 aacgcgagct gcacaccatt aagctgccgc taccgacgca gtatcagacg gctattaaag
    22021 tctccggcat tatgggcgca cgtaaaagtg cggaagatcg tgctcgcgat atgctctacc
    22081 cggagcgtat ttatcaggaa tttgaaggtg ataacgccac ctggtggaac ttcgatccgc
    22141 gcgttgagtg gctgatgggc tacctgacca gccatcgctc tcagaaagtg ctggtgatct
    22201 gcgccaaagc tgccactgcg ctgcaactgg agcaggtact gcgcgaacgt gaaggtattc
    22261 gcgctgcggt gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct gccgcctggt
    22321 ttgccgaaga agacaccggc gcacaggtac tgctgtgctc agaaatcggt tctgaaggac
    22381 gtaactttca gttcgccagc cacatggtga tgtttgacct gccattcaac ccggatctac
    22441 tggagcagcg tattggtcgt ctggatcgta tcggccaggc gcacgatatt cagatccatg
    22501 tgccttatct ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac gaaggtctgg
    22561 atgcatttga gcacacctgc ccgaccggac gcactattta cgatagcgta tacaacgatc
    22621 tgattaacta tctggcttca ccggatcaaa ccgaaggctt tgacgatctg atcaaaagct
    22681 gccgcgagca acatgaagcg ctgaaagcac agctggaaca gggtcgtgac cgcctgctgg
    22741 aaatccactc caacggtggc gaaaaagccc aggcactggc agaaagcatt gaagagcagg
    22801 atgacgatac caacctgatc gccttcgcca tgaacctgtt cgatattatc ggtatcaatc
    22861 aggacgatcg cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg ctggtgccgg
    22921 acttccctgg cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa gtggcgctgg
    22981 cgcgtgaaga tgcacagttt attacctggg agcatccgct gatccgcaac ggtctggatc
    23041 tgatcctttc tggcgatacc ggtagcagca cgatttcact gttaaaaaac aaagcgttgc
    23101 cggtaggtac gctgttggtg gaactgattt atgtggttga agcccaggct ccgaagcagt
    23161 tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat gctgctggat aaaaacggca
    23221 acaacctggc ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac gcggttaacc
    23281 gtcacaccgg cagcaaactg gttaacaccg tgcagcagga tgttcacgct atccttcaac
    23341 tgggtgaagc gcagatcgag aaatctgccc gtgcattgat tgatgcagcg cgtaacgaag
    23401 ccgacgaaaa actgtctgcc gagctgtctc gtctggaagc tctgcgtgca gtgaacccga
    23461 acattcgtga cgacgaactg accgccattg agagcaaccg tcagcaggta atggaaagcc
    23521 tggatcaggc aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg catcagtaac
    23581 ggagccgaaa atggggatgg aaaactacaa tccaccgcag gaaccctggt tggttatcct
    23641 gtatcaggat gaccatatta tggtggtcaa caagccgagc ggtttgttgt cagtgccggg
    23701 tcgtctggaa gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt atccgcaggc
    23761 agaatcggtg catcgtctgg atatggctac cagcggcgtg attgtagtgg cgctgaccaa
    23821 agccgcggag cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa agcagtatgt
    23881 ggcccgcgtc tgggggcatc catcccccgc agaaggtctg gtggatctgc cgctgatttg
    23941 cgactggcca aaccgcccga aacagaaagt ctgttacgaa acgggtaaac ctgcgcagac
    24001 ggaatatgaa gtggtggagt atgcggcgga taacacggca agagcggtgt taaaaccgat
    24061 taccgggcgt tcgcatcaat tgcgtgtgca tatgctggcg ctggggcatc cgattctcgg
    24121 cgatcgtttt tatgcatcac cagaagcgag agcgatggca ccgcgtttgt tgctacatgc
    24181 agagatgttg acgattaccc atccggcgta tggcaatagt atgacgttta aagcgccagc
    24241 ggatttttaa tcgtcacgct ggcgcagttc ggtaggcgga taaggcgttc acgccgcatc
    24301 cggcaattgt gcgccgattg cctgatgcga cgcgggccgc gtcttatcag gcctacagtt
    24361 taaaacttat ttaaaccctt tctgctgctt tatcagctca tatgcctgct gaatttcctg
    24421 cgctttctgc ttcgccatct ccatcatctc aggcggcaaa cctttcgcca ccagcttatc
    24481 gggatggtgt tcactcatca gcttacggta ggcacgtttg atggtggtcg catcatccgt
    24541 cggcttcacg cccagcacat tacaggcatc ttccagcgtc gggccacgct gcgcttgctg
    24601 ccagttacca ccgccagatt gctgctgata accgccgcca aactgtgcac cgccctgcat
    24661 catgcgcaaa aactggtcaa actgggcgcg ggagatccct aattcttcag caatgacata
    24721 cagcaccgcc cgttcattcg ggtgcagtga accatcagca aacgccgcct gaatctggat
    24781 ctccagaaac atacgaatta agtcaaaacg accaaagcag acactgcgaa actggcgcat
    24841 cttttcgcgc agcgggtaat tatctgattt tcccacccgg aacgcgtttt gcgccgcagt
    24901 acgggaagcg ccatgaaggt tcattcggtc catcaactgg ctggcgatat gaatatcagc
    24961 ctccgtgacg cgacctttgg atttggttaa atgccccatc acttcaaaag tggtggcaaa
    25021 aaacagcgcc tgacgctcac gctggttggc gaaccacgcc attttacggc tacgggcttt
    25081 atcaaacata tggccaatta acaggcctaa cactacgccc caaaagccgc cgcccatcag
    25141 taaggccacg gccacgccaa tgatttttcc ccaatactgc atatattccc caaatcgaca
    25201 cacggatatc agggctatct cccacaatat aaaggtgctt ttaccgtttt ccggctgcgg
    25261 tcaattgtgg gacatcgcct ataatttgca ttatcatacc tgtcattcaa tgccgtgcct
    25321 aacaccactg acgctattcg gacaggatta acactagcgt agagatgacg agtacgttag
    25381 tctctgaccg tttgtcacgc gcaacgttac cgatgatgga acaataaaat caacgtatga
    25441 aaaaacgtat ccccactctc ctggccacca tgattgccac cgccctttat agtcaacagg
    25501 gactggcagc cgacctcgcc tcacagtgca tgttgggcgt gccaagctat gaccgtcctc
    25561 tggtacaggg cgataccaat gacttacccg tgactatcaa tgctgaccac gcgaaagggg
    25621 actacccgga tgacgccgtg tttactggca gcgtggatat catgcagggt aacagccgtc
    25681 tgcaggccga cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg gagccggtac
    25741 gtaccgttga tgcgctcggt aatgtccatt acgacgataa ccaggtgatc ctcaaagggc
    25801 cgaaaggctg ggcgaatctg aacaccaaag atacctacgt ctgggaaggt gattaccaga
    25861 tggtgggtcg ccagggtcgc ggtaaagcgg acctgatgaa acaacgtggc gaaaaccgct
    25921 ataccattct ggataacggt agctttacct cctgtctgcc gggttctgac acctggagcg
    25981 tggtaggtag cgaaattatt catgaccgcg aagaacaagt tgcggagatc tggaacgccc
    26041 gctttaaggt gggtccggta ccgatctttt atagccccta tttgcagttg ccggtgggtg
    26101 acaaacgtcg ctctggtttc ttgatcccga acgccaagta caccaccacc aactactttg
    26161 agttctacct gccatattac tggaacatcg cgccaaatat ggatgccacc atcacgccgc
    26221 attatatgca tcgtcgtggc aacatcatgt gggagaacga attccgctac ctctcccagg
    26281 cgggcgctgg cttgatggaa ctggactatc tgccttcaga taaagtctat gaagatgaac
    26341 acccgaacga tgacagttca cgtcgttggt tattctactg gaaccactcc ggggtcatgg
    26401 atcaggtgtg gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc tacttcaatg
    26461 atttcgataa caagtacggt tccagtactg acggctacgc aacgcaaaaa ttcagcgttg
    26521 gctatgcggt gcaaaacttc aatgccaccg tttcaaccaa gcagttccag gttttcagcg
    26581 aacagaacac cagtagctac tcggcagagc cgcagttaga cgttaattac taccagaatg
    26641 atgttggtcc gtttgatacg cgtatttacg gccaggcagt gcactttgtt aacaccagag
    26701 acgacatgcc tgaagcaacc cgtgttcacc tggaaccgac catcaatttg ccgctctcta
    26761 ataactgggg cagcatcaat accgaagcga agtttctggc aacccattat cagcaaacca
    26821 atcttgactg gtataactcc agaaacacga ccaagctgga cgaatccgtt aaccgcgtaa
    26881 tgccgcaatt caaagttgac ggcaaaatgg tctttgaacg cgatatggaa atgctggctc
    26941 cgggttatac ccaaacgctg gaaccgcgcg cgcagtattt gtacgtgccg tatcgcgatc
    27001 agagcgacat ctataactac gactcgtctc tgctgcaatc tgactactct ggcctgttcc
    27061 gggaccggac ttacggcggt cttgaccgta ttgcctccgc taaccaggtg acgaccggtg
    27121 tcacatctcg catatatgat gatgctgccg ttgaacgttt taatatttcc gttggtcaaa
    27181 tctactattt cacggagtct cgcactggcg atgacaacat aacatgggag aatgacgaca
    27241 aaacgggctc actggtgtgg gcaggcgaca cctactggcg tatctccgag cgttggggat
    27301 tgcgtggcgg gattcagtac gatacacgtc tggataacgt agcgaccagt aactccagca
    27361 ttgaataccg tcgggatgaa gaccgtctgg tacagctgaa ttaccattac gccagcccgg
    27421 aatatattca ggctacgctg cctaagtact attccactgc tgagcaatat aagaatggta
    27481 tttcgcaggt aggtgctgtc gccagcaggc caattgccga tcgttggtcc attgttgggg
    27541 cctactacta cgacaccaat gcgaacaagc aagccgactc tatgttaggt gtgcaataca
    27601 gctcctgctg ctatgcaatt cgcgtcggtt acgagcggaa gctgaacggt tgggataacg
    27661 ataaacaaca tgcggtatat gacaacgcaa tcggctttaa catcgaactt cgcggcctga
    27721 gctccaacta cggtctgggt acgcaagaga tgctgcgttc gaacattctg ccgtatcaaa
    27781 acactttgtg atttgttgat ttaccacgta atccgcagtg cggttaattg aaatggaaaa
    27841 agtatgaaga actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa taccagtttc
    27901 gctgcccccc aggtagtcga taaagtcgca gccgtcgtca ataacggcgt cgtgctggaa
    27961 agcgacgttg atggattaat gcagtcggta aaactgaacg ctgcgcaggc aaggcagcag
    28021 cttcctgatg acgcgacgct gcgccaccaa atcatggaac gtttgatcat ggatcaaatc
    28081 atcctgcaga tggggcagaa aatgggagtg aaaatctccg atgagcagct ggatcaggcg
    28141 attgctaaca tcgcgaaaca gaacaacatg acgctggatc agatgcgcag ccgtctggct
    28201 tacgatggtc tgaactacaa cacttatcgt aaccagatcc gcaaagagat gattatctct
    28261 gaagtgcgta acaacgaggt gcgtcgtcgc atcaccatcc tgccgcagga agtcgaatcc
    28321 ctggcgcagc aggtgggtaa ccaaaacgac gccagcactg agctgaacct gagccacatc
    28381 ctgatcccgc tgccggaaaa cccgacctct gatcaggtga acgaagcgga aagccaggcg
    28441 cgcgccattg tcgatcaggc gcgtaacggc gctgatttcg gtaagctggc gattgctcat
    28501 tctgccgacc agcaggcgct gaacggcggc cagatggggt ggggccgtat tcaggagttg
    28561 ccggggatct tcgcccaggc attaagcacc gcgaagaaag gcgacattgt tggcccgatt
    28621 cgttccggcg ttggcttcca tattctgaaa gttaacgacc tgcgcggcga aagcaaaaat
    28681 atctcggtga ccgaagttca tgctcgccat attctgctga aaccgtcgcc gatcatgact
    28741 gacgaacagg cccgcgtgaa actggagcaa attgctgctg atatcaagag tggtaaaacg
    28801 acttttgctg ccgcagcgaa agagttctcg caggatccag gttctgctaa ccagggcggt
    28861 gatctcggct gggctacacc agatattttc gatccagcct tccgtgacgc cctgacccgc
    28921 ctgaacaaag gtcaaatgag tgcaccggtt cactcttcat tcggctggca tttaatcgaa
    28981 ctgctggata cccgtaatgt cgataaaacc gacgctgcgc agaaagatcg tgcataccgc
    29041 atgctgatga accgtaagct ctcggaagaa gcagcaagct ggatgcagga acaacgtgcc
    29101 agcgcctacg ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt gtgatcactc
    29161 ccggcgagcc cgccgggatt ggcccggact tagttgtcca gcttgcacag cgtgagtggc
    29221 cggtcgaact ggttgtttgt gccgatgcca ctctccttac cgaccgggca gcgatgctcg
    29281 gtttgccgct caccctccgc ccttattccc ccaactcccc tgcacaaccg caaactacgg
    29341 gcacattaac gctacttcct gtcgcgctac gtgaatctgt cactgcgggg cagttagcga
    29401 ttgaaaatgg gcattacgtg gtggagacgc tggcgcgagc gtgtgatggc tgtctgaacg
    29461 gtgaatttgc tgcgctgatc acaggccccg tgcataaagg cgtcattaac gacgcaagca
    29521 ttccgtttac cggtcatacc gagtttttcg aagagcgttc gcaggcgaaa aaagtggtga
    29581 tgatgctggc gaccgaagaa cttcgcgtgg cgctggcaac gacgcattta ccgctgcgcg
    29641 atatcgcaga tgctatcacc cctgcacttt tgcacgaagt gattgctatt ttgcatcacg
    29701 atttgcgtac aaaatttggt attaccgaac cgcgcattct ggtctgcggg ctgaatccgc
    29761 acgcgggcga aggcggtcat atgggtacgg aagagataga caccattatt ccggtgctcg
    29821 acgagctgcg ggcgcagggg atgaaactca acgggccgct gcctgccgat accctgtttc
    29881 agccgaaata tctcgataac gccgacgccg tgctggcgat gtaccacgat cagggtcttc
    29941 ccgtgctaaa ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc ctgcccttta
    30001 ttcgcacatc agtggaccac ggcaccgcgc ttgaactggc gggacgtggc gaagccgatg
    30061 tcggcagttt tattacggcg cttaatctcg ccatcaaaat gattgttaac acccaatgaa
    30121 taatcgagtc caccagggcc acttagcccg taaacgcttc gggcaaaact ttctcaacga
    30181 tcagttcgtg atcgacagca ttgtttctgc cattaacccg caaaagggcc aggcaatggt
    30241 cgaaatcggc cccggtcttg cggcattgac cgagccggtc ggcgaacgtc tggaccagct
    30301 gacggtcatc gaacttgacc gcgatctggc ggcacgtctg caaacgcatc cattcttagg
    30361 cccgaaactg acgatttatc agcaggatgc gatgaccttt aactttggcg aactggccga
    30421 gaaaatgggt cagccgctgc gtgttttcgg caacctgcct tataacatct ccacgccgtt
    30481 gatgttccat ctgtttagct atactgatgc cattgccgac atgcacttta tgttgcaaaa
    30541 agaggtggtg aatcgtctgg ttgcaggacc gaacagcaaa gcgtatggtc gattaagcgt
    30601 catggcgcaa tactattgca acgtgatccc ggtactggaa gttccgccgt cagcctttac
    30661 accaccaccc aaagtggatt ccgccgtcgt gcgcctggtc cctcatgcaa cgatgcctca
    30721 cccggttaaa gatgttcgcg tgttgagccg catcaccacc gaagccttta accagcgtcg
    30781 taaaaccatt cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa ccggaatggg
    30841 gatcgacccg gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc agatggcgaa
    30901 ctatctggcg gagaacgcgc ctttgcagga gagttaacga tgatcaattc gccccgagtg
    30961 tgtattcagg ttcaaagcgt ctacattgag gcacaatctt cacctgataa tgaacgttac
    31021 gtttttgctt ataccgtaac catacgcaat ctggggcgag cgccagtgca gttgttgggg
    31081 cgttactggc tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg cgaaggagtg
    31141 gttggcgtcc agccacttat cgcgcctggc gaagagtacc agtacaccag cggtgcaatc
    31201 attgaaaccc cgctgggcac catgcagggt cactacgaaa tgatcgatga aaatggcgtc
    31261 cctttcagca tcgacattcc cgtattccga ctcgccgttc ccacactcat tcattaaaag
    31321 aatatggcga cataccttat tggcgacgtt catggttgtt acgatgaact gatcgcattg
    31381 ctgcataaag tagaatttac ccccgggaaa gataccctct ggctgacggg cgatctggtc
    31441 gcgcgcggcc cgggttcgct ggatgttctg cgctatgtga aatccttagg cgacagcgta
    31501 cgtctggtgc tgggcaatca cgatctgcat ctgctggcgg tatttgccgg gatcagccgc
    31561 aataaaccga aagatcgcct gacaccgctg ctggaagcgc cggatgccga cgagctgctt
    31621 aactggctgc gtcgccagcc tctgctgcaa atcgacgaag agaaaaagct ggtgatggcc
    31681 cacgcaggga tcacgccgca gtgggatctg cagaccgcca aagagtgcgc gcgcgatgta
    31741 gaagcggtgc tgtcgagtga ctcctatccc ttctttcttg acgctatgta cggcgatatg
    31801 ccaaataact ggtcaccgga attgcggggg ctgggaagac tgcgttttat caccaacgcc
    31861 tttacccgta tgcgtttttg cttcccgaac ggtcaactgg atatgtacag caaagaatcg
    31921 ccggaagaag cccctgcccc actgaaaccg tggtttgcga ttcctggccc tgtcgctgaa
    31981 gaatacagca tcgcctttgg tcactgggca tcgctggaag gcaaaggtac gccggaaggt
    32041 atttacgcgc tggataccgg ctgctgctgg ggtgggtcat taacctgcct gcgctgggaa
    32101 gataaacagt attttgtcca gccgtcgaac cggcataagg atttgggcga agcggcggcg
    32161 tcttaaacac agcctgatac aggaaggccg gataagacgc gaccggcgtc gcatccggca
    32221 ctagccgtaa attctataca aacttaccgc cgctccagaa tctcaaagca atagctgtga
    32281 gagttctgcg catcggcgtc gtggaattcg ctgaataccg attcccagtc atccggctcg
    32341 taatccggga aatgggtgtc gccttccact tctgcgtcga tatgcgtcag atacagtttt
    32401 tgcgcttttg gcaggaactg ctcataaacg cgaccgccgc caatcaccat gatttctggt
    32461 acgtcaccac acgccgcaat ggcttcatcc accgatttca cccacgttac gcgatcgtcc
    32521 gtacccggtt gactgctgag gataatattt ttgcgtcctg gcaacggacg accgattgat
    32581 tcccaagtat ggcggcccat aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg
    32641 agatcggcag gcaggttcca cggcatggcg ttttccatgc cgataacgcg atctaccgct
    32701 aacgccgcaa tcagactgat cattgagatt tcccgataaa aaaattgtcg ccactatacg
    32761 taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt ttatattctg
    32821 ctggcgagtc cacgctctcc ccctggactc gccgcattac aatgaaacaa aaacaaacag
    32881 ttagctgtaa agtgtgattt acgtcactct ttattaagat gagggtttcg tttccggttc
    32941 atccgccatg ttgccggtat gtttaccttc ttctgttccc tgccagccat gtcgttgaat
    33001 taatgacaga tgttcgcggt cctcggtgat gatctcactt aacatcgcgc tggtgcgttt
    33061 atagaccgcc gcgcgggctt tggtgtcatt ctcaaccatt gccatctctt ccaccatctg
    33121 aatattaaag cggcggaaca catcggcacg ttctcgcgct tcatacggcc ccagacctaa
    33181 actttccagc gccagacgcc cggttttcag cgcaccttca aaggtttcac gctccggctt
    33241 ttcaacgcct gcctgacgca aacgaatgta gtgatcgaca tcgcgggcgc gggcaataat
    33301 ctgcaaatgc gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg tttgcggatc
    33361 gtcaatagcg ttaatcagca cttccgcttt cgccgctccg gcagattcca gtaaatccat
    33421 ccgcgtggca tcgccataaa acactttcat accaaattta cgcagcgttt cgatatggtc
    33481 cggatcgtga tcgagcacca ccattttcac cccgctggag agcagtaaac gtccggtaat
    33541 ctgtccaaaa cgaccgaatc cggcgataat cacgcgcggc tgttcttcat cgatctcatc
    33601 ggcttcacgc gcctcctccg tagaagattg ctcaaggcga ttgaggatca ccagcagaat
    33661 cggcgttgct gccatcgaca gcgccaccgc cagggtcagc gatttcgccc actccggctc
    33721 cagcacattc gccatctgcg ccgcaccaaa taccacaaag gcaaactcac tgccctgccc
    33781 taacaacacc gcaaaccagc gacgctgttt atttggcacc tgcaacggtc gggcaatcag
    33841 ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa tgcgcaatgg
    33901 gttttcaatc agcgtgccaa agtctatcga catgccaaca ccgatgaaaa acagccccaa
    33961 cagcaaacct ttaaacggtt cgatatcgct ttccaacgca tgacggtatt ccgagcttgc
    34021 cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct cttccagcag
    34081 caaaccaaag ccaaacacaa ggaataacgc cacggcacta aacacttccc gcaagccaga
    34141 gcgggcaaca aaacgcagcg ccggacgcgt aacatagcgc cccagcaata ccaccagtac
    34201 cagcgcaccc gccactttta acgccgagag agcaaatgcg cccatcgtcg tcgaggcact
    34261 gctcgccgcc agtagcggga tcatcgccac cagcgggatt gccgcgatat cctggaacag
    34321 cagcactgca aaggcactgc ggcccatttg cgtcaccatc agattgcgtt cattcatcgc
    34381 ctgcatggca atcgccgttg atgagagcgc cagcgtcata ccgatcaatt ccgcgacctg
    34441 ccagcgcagc ccaagtaaca tacagaacag ccccagcagg ccaccgcaaa tcaccatctg
    34501 caatgcgcca ccgccgaaca ctgccgcacg cagcttccac agcctttgtg gatcgagttc
    34561 gaggccgata ataaacagca tcagcaccac cccaatctcg gcaaagtgca gaatagattc
    34621 ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga tcaggtagcc
    34681 aagtaccgat cccagaccaa gacgtaccgc aatgggtaca atcagcgccg ccgatccgag
    34741 gtagatcagc gcctgaacca gcgtatgact atccatgatg ggcctcctgc cattccagca
    34801 gacgttgctt atagtggcgc gcctgccctt cgagggtttc gtcgtcacaa ataaaggtgc
    34861 agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc gcctgtagcg
    34921 gctgcgacag cacatcaaag cccggatgcg caccaatttc aaaatggctt tccccgccgc
    34981 cggtcgtcac cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg tgaccgtaag
    35041 cccagccgtg cgacaaaact ttatcgatcc aaagtttaag aagcggagga atgctgtacc
    35101 actgcatcgg gtgctgccag acgatcagat cggcgcgaga gagcgcctcc tgctcggcgg
    35161 caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg acgccttcca
    35221 gcgtccttgc ctgttcaagc atccgtttat tcgcatggga atgatgcgga tacggatgcg
    35281 cataaatgat gaggatcata gttattagcc cgcgtgttta cgcctttgtt ttcccacaaa
    35341 gtgtagtcat atattcacgc ggcttatagt caataatatt gattcagaaa agagagaaat
    35401 ctgaacaaat aatcccattc tctgcccgcg gatcgtcata ttgctggtct gcgccagtga
    35461 cattcctcga gtctccgggg caaacgctac ggaaatcaac aagccaaaca gcgagatacc
    35521 cgcccccatt agcatcgtgt tactgatacc gtaattattg ataaatatcg gcagtgccca
    35581 ggtcgaaaca atggtgccaa tacgacttaa ggacataatc acgcccacgg cagaggcgcg
    35641 gatatccgtc gggaagagtt cattaggata gagccactgc aaattacccg gcccgccaga
    35701 gaaaaaggca tacaccgcaa aggccatcac taccagccag atccccatat ccgggattag
    35761 ccccaaaacc gccagcgcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg
    35821 tccggcagtg tttagccaca gcatcggcgg aatacagccg agcataaaga acaggctaat
    35881 caccacattc cctagtgccg cgtttttgcc aacccccaat cccaacgaac caacgatttg
    35941 cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg caggtccaga tggcggcaac
    36001 aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg
    36061 ctgctcttca tcgaaagcca ccggttcgcc aaacagtttt atcatcatct cttcgcactc
    36121 ttttactcgc cctttgcgta ataaccagcg gggagattct ggcagttcga atcgaccaat
    36181 caaaatcaac aaacaaggga tcgccgcgct acccagcatc cagcgccaac caccttccac
    36241 atcataaagc cagtagccga ccagatcggc gcaggtcgcg ccgacatacc acatcgcggc
    36301 aataaagctg atggaaaacg cccgctgacg ggtactggag aactcggtga tcatcgaagt
    36361 ggcgatggga taatctgcac cgatgacaat gccgatgagt acccgcatca ccaacagttc
    36421 gacgggggat gaaacacaca tcgtcgccac cgatatcacg ccgatggcga tgatatcaat
    36481 gaggaacatt ttgcgccgtc cgactttatc ggagatatag ccaaacagcg atgtgccaac
    36541 gaacagcccg gcgagcgttc ccgcgcccag caagccaatc cagtcagcgt ccagtttcag
    36601 cgccggagtc agttgctcca gcgccacgcc aatcattacc agtacataac catccagaaa
    36661 cggaccaccg cttccccaca gcaaaatgcg gcggtgaata gaggagaatt tgagatcgtc
    36721 aaagtttctg gacggttgca tgggttattc ctgttttttt gcaatgagag tcagtacggc
    36781 gttacgtatc aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt
    36841 attcccactg ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac
    36901 atccggcgta atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc
    36961 acgctttcac cagcagctcc agcacctgtt tatcagcatc ggccttcaca acaatgtgcg
    37021 gatgctcttc atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag
    37081 aagtcataaa acggtcactc ctttcatgcc atccttgatc agattgatga agcccacttt
    37141 cttgccgtgg cggaggattt tcttgcgcat cagttccggc gcactgccgt caatggtgaa
    37201 caggtcacgc gccacgccca ccgccagttc cgggtagccg ctaaacatgc gtgggttatc
    37261 aaggaacgcc ggtagtttct ggtacatgcg catatcgcgc agcgggctgc tctccagatg
    37321 ctgacgatat tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac
    37381 ggtttttgct gcagcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc
    37441 gaggttcata cacattccgg cggcatcacc ggcaatcaat acgccgtcac cgaccagctc
    37501 cggcagcatg ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc
    37561 gcccgcgatc agcggtgcaa cggccggatg ctgtttgaaa tcttccagca tttgcggcac
    37621 cgattttttc gcgtcatgca gatgatgcag accacaaacc agccccagcg acagggtatt
    37681 ttcattggta taaaggaagc cgccgcccat caggccatcg gtgggtgatc ccgcaaacag
    37741 acaagccgcg ccctgattac cctgcaactg aaaacggtct tcgattaccg acttcggtga
    37801 ctcgatcagt tccttcacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc
    37861 caacttttcg gcaaggatgg agttcacccc atcagcaagg agcaccgttt tcgcttcaat
    37921 cacatcgcca tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac
    37981 gcggatcccg gtaattaact gcgcgcccgc ttcttcggcc tgctccatca accaggcatc
    38041 aaatttactg cgcaaaacgg agtaagaacg ctgggatagc gaggtttcgt caccgttgca
    38101 gtagtccata gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag
    38161 gcgttctacg gggcggaatc ggcgaaacct ggaataatgt gttccagact gtgggcatag
    38221 agacgcccgc cagtaacgtt cttggcaccc gcggaattgc cacgctcgat aactaacact
    38281 tgcgcccctt cgcgggcgag caccagtgcg gcaaccgaac cggcaagccc tgcaccgacg
    38341 atgatggcgt caaagatatc ttcggacata agagctccct gtcagcggca gagccgtaat
    38401 gcagccctgc cagagtggat caacgcgcta aagctgcggt cagtgccgga aggatcttca
    38461 cggcatcgcc aacaatgccg taatccgcgt actggaagat cggcgcattt ttatctttat
    38521 tgatggcgaa aatggtttgc gacgcgttag cgccaaccat gtgctggatc tgcccggaga
    38581 tcctcaccgc caggtacagt tcaggtttca gcatcaggtt ggagataccg acatagcgtt
    38641 cgtgctccat ccatttttcg ttttctgcca ccggacgaga acaggccaac tccgcaccta
    38701 tcgccttgca aagctgttct gccagtgcaa tgttctcttt gctgccaatg ccgcgaccga
    38761 cgctgaccac cagacgggct ttgtcgagat cgacgctgtt gctctggcgc gtctgggttg
    38821 ccgtgcgggt aatcgccaca gccggagcct gccactccac ggtgtgcgtt tcgccagtgc
    38881 gtgacgcgtc tggctgagcc gcatcgaacg tgccgctgct gatggtcagt accgcatacg
    38941 gcgtggcaat gcgttcttcg ccaatcgcca gaccgccgta aaccatgtgt ttcactgttg
    39001 ctttaccgtc ctgtacgctg acggtgctgg catcgttaga caccgccgct ttaaggcgat
    39061 aacccagttt tgccgccagt aatttgccgc gacgggtgtt tggcaacagc accagaccgt
    39121 cagcgccgtg ctggcgaata gtgtcagcca tgacaccggc gtaatcttcg atcatccgat
    39181 cgtccggttt gccgtttaat ttccagacat gattagcgcc gagctggatt gcctgtgcgc
    39241 cgtcggcatc attgaggaca aaggcattga tttgattagc taaagcctgc gcaccgttca
    39301 tcagttccgg cagacgagag ggggtatcgc tgaataccca gacttgagaa aacgtgttca
    39361 tagcatcccc tgtaattaaa tgactttgcg aagattttca gcaaatgcgg cgatctgttc
    39421 ttcgccgtcg ccttcaatca cgatgcgctg acgctcgcgc tgtttcggcg cggcaacctg
    39481 ttgttcagac caggcgtcca cggcgttaaa accaatatcc gccgccgacc atacctggac
    39541 tggctttttc gccgcgccga gaatggcttt catcgaagga atttgtgggg agttgatatc
    39601 agtggaaaca gcaacaaccg caggcagcgg aatgcttaag gtttcggttt catcttccag
    39661 ttcgcgctca acggtgaggg tatctgccgt cagggagata attttgctga cgccgttaac
    39721 tgccggaata ttgaggattt cgcccaccag cagaccaacc tgctgggcat aaaggtcgga
    39781 agaaccatcg ccacagagga tcagatcaaa gcctgctttc tgggcggctg cagccagtgc
    39841 gctcgccgtt tgttgcggca gtgcctgctc gaactggtca tcaatcacca caatcagttc
    39901 atccgggccg cgcgatagca catctttacg ccctttggcg ttggtcaggg ctttaccgcc
    39961 cacacttaag gctgtcacct gcgcctctgc tgcctgttgc tttagctggc aagccgcttc
    40021 aatagcgttg agatcgtatt ggcttatttt ggcatcggct ttgctgaagt ctaatgaacc
    40081 atcagcatta ttgaccgcaa tatcctgttc atcaggcacg cacttatagc aagtaataat
    40141 cttcattgca tctccagaaa tcatgaagga aacgtcgttc atatcggtgg tgttaattac
    40201 agatatcacg ccctttaatt tggaaataaa actttaatca ccaatattga aaatgtcaca
    40261 cgcagataaa tccaactttc aatattgtta agttcctcac caatattgaa aacatggcgt
    40321 agcaaaaaga aattttcaat attgttttat agatcaccaa tattgaaagt cagatgctat
    40381 tttttaccca caaaagaaca taacaaactg attaataacc aaaattaacg aaaaacatcc
    40441 tctattcact cgaaacaaaa atgtgacacc aatcacagaa tacagcttat tgaataccca
    40501 ttatgagtta gccattaacg cgtccacgag gttaataata attatattaa atgttaacaa
    40561 aaataaaaca aacgggaacg caaaataaat attcgttttc acagtggaat taactcatga
    40621 agaatgaaaa gagaaaaacg ggaatagaac cgaaggtttt ctttccgcca ttaataatcg
    40681 tcggcatact ttgttggctt acagtcagag atctggatgc ggcgaatgtc gttattaatg
    40741 ctgtattcag ttacgtcacc aatgtatggg gatgggcatt tgaatggtat atggtggtga
    40801 tgcttttcgg ttggttctgg ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg
    40861 aaccgccaga atttagcacc gccagttgga tctttatgat gttcgcctcc tgtacgtctg
    40921 ctgccgtact gttctgggga tcgattgaga tctactacta catctccacc ccgccgtttg
    40981 gcttagaacc gaactcgaca ggagcgaaag agttggggct ggcttacagc ttgttccact
    41041 ggggacctct gccgtgggcc acttacagct tcctttcagt cgccttcgct tacttcttct
    41101 ttgtccgcaa aatggaagtg attcgcccca gctcgacact ggtgccgctg gtaggtgaaa
    41161 aacacgccaa agggttgttc ggtaccatcg tcgacaactt ctatctcgtc gccttgatct
    41221 tcgcgatggg taccagtctg ggccttgcca cgccgctggt gaccgagtgt atgcaatggt
    41281 tgtttggcat tccgcatacc ctgcaactgg acgctatcat cattacctgc tggattatcc
    41341 tcaacgccat ttgcgtcgct tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc
    41401 gtagttacct gagcttcctg atgctgggtt gggtgttcat cgtcagcggt gccagcttca
    41461 tcatgaacta cttcaccgat tcggtgggga tgttgctgat gtatctgccg cgcatgttgt
    41521 tctataccga tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg ttctactggg
    41581 catggtgggt gatttacgcc atccagatga gtatcttcct cgcccgcatc tcccgtggtc
    41641 gtactgtgcg tgaactgtgc ttcggcatgg tgatggggct gacagcatcc acctggatcc
    41701 tgtggaccgt gctcggtagt aacactctgc tgttgatgga taaaaacatc atcaacattc
    41761 caaatctgat cgaacagtac ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc
    41821 cactcagcac cgccaccatg tggggcttct tcatcctctg ctttattgcc accgttacgc
    41881 tggttaacgc ctgctcttat accctggcga tgtctacttg ccgcgaagta cgcgatggtg
    41941 aagaaccacc gctgctggtg cgtatcggtt ggtcaattct ggttggcatt atcggtattg
    42001 ttctgctggc gctcggcggc ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc
    42061 cgctgttctt cgtcaacatt atggtgacgc tctcctttat taaagacgcg aaacagaact
    42121 ggaaagatta attaaccccc aaaatatcaa gaggttgaaa gatggatttt aatttaaatg
    42181 atgagcagga actgtttgtc gccggtatcc gcgaactgat ggccagcgaa aactgggagg
    42241 cctattttgc cgagtgcgac cgtgacagcg tctacccgga acgttttgtc aaagcactgg
    42301 cggatatggg tatcgacagt ctgctgatcc ctgaagagca cggtggtctg gacgcggggt
    42361 ttgttactct cgccgccgtg tggatggagc tgggacgtct gggggcacca acctatgtac
    42421 tgtaccagct gccgggcggg ttcaatacct tcctgcgcga aggcacacaa gagcagatcg
    42481 acaagattat ggctttccgc ggcaccggta agcagatgtg gaactcagcg attaccgaac
    42541 cgggcgcggg ctccgacgtg ggtagcctga aaacgactta tacccgtaga aatggtaaga
    42601 tttatcttaa tggtagtaag tgttttatta ccagtagcgc ctacaccccg tacatcgtgg
    42661 tgatggcgcg cgacggggct tctccggaca aacctgtcta caccgaatgg tttgttgata
    42721 tgagcaaacc gggcatcaaa gtgaccaaac ttgagaagct cggtctgcgt atggatagct
    42781 gctgtgaaat cacctttgac gatgtggaac tggacgagaa agacatgttc ggtcgggaag
    42841 gtaacggctt taaccgcgtc aaagaagagt tcgaccatga acgtttcctg gtagccctga
    42901 ccaactacgg tacggcgatg tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg
    42961 tgcagtttgg cgaggctatt ggtcgtttcc agttgattca ggaaaaattc gcccacatgg
    43021 cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg
    43081 gcaccatcac ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg
    43141 aagtggtgga tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca
    43201 tcagccgctt ctggcgtgac ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc
    43261 agatcctgac gctgggtcgt gcggtgctga agcaataccg ctaagtccgt gcgctgcctg
    43321 atgcgacgct gacgcgtctt atcaggccta cgggagcacg aatgtaagcc ggataaggcg
    43381 tttacgccgc atccggcagt catgcgctac ataaattttt caggagctaa taatggatca
    43441 tctacccatg ccgaaattcg gaccgctggc cggattgcgc gttgtcttct ccggtatcga
    43501 aatcgccgga ccgtttgccg gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat
    43561 cgagaacgtc gcctgggccg acaccattcg cgttcaaccg aactacccgc agctctcccg
    43621 ccgcaatttg cacgcgctgt cgttaaatat tttcaaagat gaaggccgcg aagcgtttct
    43681 gaaattaatg gaaaccaccg atatcttcat cgaagccagt aaaggtccgg cctttgcccg
    43741 tcgtggcatt accgatgaag tactgtggca gcacaacccg aaactggtta tcgctcacct
    43801 gtccggtttt ggtcagtacg gcaccgagga gtacaccaat cttccggcct ataacaccat
    43861 cgcccaggcc ttcagtggtt acctgattca gaacggtgat gttgaccagc caatgcctgc
    43921 cttcccgtat accgccgatt acttttctgg cctgaccgcc accacggcgg cgctggcagc
    43981 actgcataaa gtgcgtgaaa ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt
    44041 gatgctgcgt atgggccagt acttcatgat ggattacttc aacggcggcg aaatgtgccc
    44101 gcgcatgagc aaaggtaaag atccctacta cgccggttgc ggtctgtata aatgtgccga
    44161 cggctacatc gtgatggaac tggtgggcat tacccaaatt gaagagtgct ttaaagatat
    44221 tggcctcgca catctactcg gtacgccgga aatcccggaa ggcactcagc ttatccaccg
    44281 tatcgaatgc ccttacggcc cactggtaga agaaaaactc gatgcctggc tggcggcaca
    44341 taccatcgca gaagttaaag aacgctttgc cgaactgaat atcgcctgtg ccaaagtact
    44401 gaccgtaccg gaactggaaa gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg
    44461 gcaaacgatg gatggtcgca cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa
    44521 ccccggacaa atctggcgcg gaatgccctc acatggcatg gacacggctg ccattttgaa
    44581 aaatatcggc tacagcgaaa acgacattca ggagttggtc agcaaaggtc tggccaaagt
    44641 tgaggactaa acgcgtggcg tcagtcggct tgcctgacta gcgcccgccc aataacaaac
    44701 gaacaaaaat ggatagaggt gcaatggata tcattggcgg acaacatcta cgtcaaatgt
    44761 gggacgatct ggcggacgtt tacggtcata aaacggcgct gatttgtgaa tccagcggcg
    44821 gagtcgttaa ccggtatagt tatcttgagt taaatcagga gattaaccgc acggcaaacc
    44881 tgttttatac gctggggatt cgcaaaggca acaaggttgc actacatctc gacaactgcc
    44941 cggaatttat cttttgctgg ttcgggctgg caaaaattgg cgcgattatg gtgccgatta
    45001 acgcccgcct gttacgcgag gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc
    45061 tggtgaccag tgcgcaattt tatcctatgt atcaacagat tcagcaggaa gatgccactc
    45121 aattgcggca catttgcctg acagatgtgg cacttcccgc tgatgatgga gtgagttcat
    45181 ttactcaact gaaaaatcaa caacctgcca ccttgtgcta tgcaccgccg ctatcgactg
    45241 acgatacggc ggaaattctc ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga
    45301 ttacccatta caacctgcgc ttcgctggat attactccgc ctggcagtgt gcactgcgtg
    45361 acgatgacgt ctacatgacg gtaatgcctg cgtttcatat cgattgccag tgtactgcgg
    45421 cgatggcggc gttttctgcc ggggccacct ttgtgctggt cgagaaatac agcgcccgcg
    45481 ccttctgggg acaggcgcag aagtaccgcg ccaccattac cgaatgtatt ccgatgatga
    45541 tccgtacgtt gatggtgcag ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag
    45601 tgatgtttta tctcaacttg tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg
    45661 ttcgcttgct gacgtcttat gggatgacgg aaaccattgt gggcattatc ggcgatcgcc
    45721 ctagcgataa acgacgctgg ccgtcgattg gtcgggcggg gttttgctac gaagcggaga
    45781 tccgcgacga tcacaatcgc ccgctcccgg caggtgagat cggtgaaatc tgtattaaag
    45841 gcgtaccagg gaaaaccatc ttcaaagagt attttctcaa cccgaaagcc actgcgaaag
    45901 tgctggaagc cgacggctgg ctacataccg gcgataccgg ataccgcgac gaagaaggct
    45961 ttttttattt cgtcgatcgc cgctgcaata tgatcaaacg cggtggcgag aatgtctcct
    46021 gcgtggagct ggaaaatatc atcgccaccc atccgaaaat tcaggacatc gtggttgtgg
    46081 gtattaaaga ttcgattcgc gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg
    46141 aaacattgag cgaagaggaa ttttttcgct tctgcgaaca aaatatggcg aaatttaaag
    46201 tgccctctta tctggagatc agaaaagatc tgccacgtaa ttgctcgggg aaaataatta
    46261 gaaagaatct gaaataaatg aaacgtcagg gaactaccct gccagctaat aatcacgccc
    46321 taaaacaata tgcatttttt gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa
    46381 aaggaatgag tgaatcatta catctgaccc gcaatggatc aattctggaa attacccttg
    46441 atcgcccaaa agcgaatgct attgatgcaa aaaccagctt tgaaatgggc gaagtatttc
    46501 taaatttccg tgacgatccg caattacgtg tcgccattat taccggtgcc ggagagaaat
    46561 tcttttccgc gggctgggat ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact
    46621 ttggcccggg tggttttgcg ggattaaccg aaattttcaa tctcgacaaa ccagttatcg
    46681 cagctgtgaa cggctacgcc tttggcggcg gtttcgaact ggcgctggcg gcagatttta
    46741 ttgtttgtgc cgataacgcc agcttcgccc tgccggaagc caaactgggc atcgttcctg
    46801 acagcggcgg tgtgctgcgt ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg
    46861 tgatgaccgg cagacgaatg ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg
    46921 tggttagcca ggcggaactg atggataacg cccgcgaact ggctcagcag ctggttaaca
    46981 gcgccccgct ggcgattgcg gcgctgaaag agatctaccg caccaccagc gaaatgccgg
    47041 tagaagaagc gtatcgctat attcgcagcg gcgtgttgaa acactatcca tcggttctgc
    47101 attcggaaga tgccattgaa gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga
    47161 aaggacgtta acaaccgtga gctattacgc ctttgagggg ttaattccgg tggttcaccc
    47221 gacggcgttt gtccatccca gtgccgtctt gattggcgat gtgattgtgg gagccggtgt
    47281 ctacatcggc ccactcgcct cactgcgtgg tgactacggg cggttgatcg tgcaagcggg
    47341 agccaatatt caggatggct gcattatgca tggctactgc gacactgaca ccatcgttgg
    47401 ggaaaacggc catatcgggc atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc
    47461 attggtcggg atgaacagcg taattatgga tggcgcggtc attggcgaag agagcattgt
    47521 tgccgcaatg agctttgtca aagcaggctt tagtggcgag aaacgccagt tgttgatggg
    47581 tacgccagcc cgcgctgtac gcagtgttag tgacgacgag ttacactgga aacggttgaa
    47641 taccaaagag tatcaggatc ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc
    47701 actgaggcaa atggaggaaa atcgcccccg tttgcagggg acgacggatg tgacgcctaa
    47761 acggtgatct tgccggatgc gacctggttg ctggataaga tgctacaaca tcgtatctgg
    47821 caagcaggac gcccggtatc aacgagataa aattaacgac gcatactctt tgacaacatc
    47881 atctgccact tctgctcgcg gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc
    47941 tttcttacgc cgactaattt ttcccgactg ttattgcgca ggcgcgcgta aatttgctca
    48001 tcgatatcga ccactttaac cagtcgctga cactggcatc cccgcccttc cagcttatta
    48061 gggatcatct taacttcgca gcttatttct gtgacttccg acagaatata agtcagggta
    48121 ttaaccgcct tgctgtgttc aatatcgaaa tgaatagaga tctctcttgc tatcacccac
    48181 cgattttcag ccatcatcca ttcggcgatt aacaagtaga gtggtttttc aacatatcct
    48241 tcacacatac gaactccagt ttcacaaatt aaaagtctgg ctatgactcg ttttgaagca
    48301 attaaacaag accatattaa aattgtggat atatcgatag tatgcaactt caccgtcgat
    48361 aagtgtgagc taaagcttat gtcatcaaaa atatcgattc cccaaaagat ttacttaatg
    48421 ggcaaaaaaa aacggtcctc atcagagaac cgtattagtt attcgatcaa attagcggat
    48481 gaaaaatatc cgccacgaca cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa
    48541 ttactttttc agtcgcatcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg
    48601 tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa
    48661 tcgcacgacg gcctgaggtg gtgttgatga tgtaggtata ttcgccattc ttgatacggt
    48721 cctgaatgtg cggacggcct tcatgcacct tgtttaccag acgcggattg atacccgctt
    48781 cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt
    48841 ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc agcgcacgac
    48901 cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg
    48961 tgcggcccac gcccatgact tccccggtag agcgcatttc tggccctaac agcgggtcaa
    49021 cgcctgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac ggcggaataa
    49081 cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt
    49141 ttgccagcgg tacgccggtg gctttggaga cgaacggaac agtacgcgcc gcacgcgggt
    49201 ttacttcaat caggtaaact tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc
    49261 gtacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc tgaatttcct
    49321 gacttaaggt gtacgctggc agcgaacatg cggagtcacc ggagtgcacg cccgcctgct
    49381 cgatgtgctc catgatgccg ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt
    49441 caacttctac tgcgtcatca aggaaatgat ccagcaacac tggcgcatcg ttagacacgc
    49501 taaccgccgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc
    49561 gaccgccgag aacgtaagac ggacgcacca ccagcgggta gccaatctct tttgccttct
    49621 caactgccat ttcaatagca gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt
    49681 caaccgcatg ctggaagcgt tcacggtctt ccgcacggtc gatagcatcc gggctggtgc
    49741 cgataaccgg tacgccagcg gcttccagtg cacgcgccag tttcagcggg gtttgaccgc
    49801 cgtactgaac gataacgcct ttcggcttct caatacgcac gatttccagc acatcttcca
    49861 gagttaccgg ctcgaagtag aggcgatcgg aagtgtcgta gtcggtagag acggtttccg
    49921 ggttacagtt aaccataatg gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta
    49981 cgcagcagta gtcgaattcg ataccctgac cgatacggtt tggaccgccg ccaagcacca
    50041 tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca
    50101 tgtaagcggt gtcggtggca aactctgccg cacaggtatc cacgcgcttg tagaccgggt
    50161 gcaggtcata ttggtcacgc agcttacgga tttccgcttc gcgtacgccc gccagttttg
    50221 ccaagcgcgc atcggcaaag cctttgcgtt tcagctggcg caggaattca gcgttcaggc
    50281 cagtgatgcc cacttccgcc actttctctt ccagacgcac cagttcttca atctgtacca
    50341 ggaaccagcg gtcaatgtta gtcaggttga agacgccgtc cacagacagg cccgcgcgga
    50401 aagcatcggc gatgtaccag atacgctctg cgcctgcgtc tttcagttcg cgacggattt
    50461 tggttaacgc ttccgggtca tccaggctca ctttcgggtc gaagccagtt gcgcccactt
    50521 ccaggccgcg cagcgctttt tgcagggatt cctgctgtgt gcgaccaatc gccatcactt
    50581 cgccaaccga tttcatctga gtggtcagac ggtcgttagc accggcgaat ttttcgaagt
    50641 tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag gccggagtac
    50701 gtccgccagt gatgtcgttc atcagttcgt ctagggtgta acccaccgcc agtttcgccg
    50761 ccactttagc aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc
    50821 gtgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacat
    50881 tggaaccacc ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg ttacgcatga
    50941 tttgatattc tttgtcagtc agcgtttggg ctggcgcgac agtgatggag tcaccggtgt
    51001 ggatgcccat cgcatcaaag ttttcgatag agcagacgat gatgcagttg tcgtttttat
    51061 cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca atcagcaact
    51121 ctttggtcgg agagagatcc agaccgcggg cgcaaatttc ttcaaactct tcacggttat
    51181 aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc
    51241 ccacgtcagc ggcaacagcc agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg
    51301 tttccagacc aattttcttc atcgctacgt cgaaacgacg gcggtcttct gctttatcaa
    51361 tcgcatcggc agtggcacca atcatggtga caccgaactc ttccaacacg ccctgacgtt
    51421 ccagctccag cgcgcagttc agcgccgtct gaccgcccat ggttggcagc accgcgtccg
    51481 ggcgctcttt ttcaataatc ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg
    51541 catcggccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc
    51601 ggtaaccctc ttcgcgcagg gctttacagg cttgcgcgcc agagtagtca aactcacacg
    51661 cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt
    51721 ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct caattaactt
    51781 gataaagtgg tcgaacaacg gcgcggcgtc gtgtggacca gggctggctt cagggtgacc
    51841 ctggaagctg aacgccggtt tatcgatacg atgaatgccc tgtaacgtac cgtcgaacag
    51901 ggatttatgc gtgacacgca ggtttgcagg taatgttgct tcatccaccg caaaaccgtg
    51961 gttctgggcg gtgatcatca ccacgttttt ctccacatct ttaaccggat ggttgccgcc
    52021 gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca gcagctgatg
    52081 accgagacag atgccgaata ccggaatatc ggtttcgagg aacttctgga tggcggtaat
    52141 ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc cgtctggatt
    52201 cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc gacagcctct
    52261 atccaccagc atccgcagga tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa
    52321 cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc agctcccttg
    52381 tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc cgttcagacc
    52441 tgggaacgcg cgggcttttt ctaacgccag cgccgcatcc gggttatcgc ctgcgataat
    52501 gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc
    52561 ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa
    52621 gttgctggca atcagcggca ggtcgcgaat caccagacct tgtgcatgta cctgagaaga
    52681 ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa gagtaacgat
    52741 ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa
    52801 aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc
    52861 gtcttccaga accaatagcg ctgacttaat caaaacaccc tccatagaat attcactcac
    52921 tttatttgca tattaattca cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga
    52981 atttctggca aacggcggca ttctggagat atgggcgcta aaagtcaact taatggcagg
    53041 attttttata ttattttgtg gctcatgcat gttttacagg cattaagcga caaaaacatc
    53101 atctaacctg ttctggaaaa cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc
    53161 aggaaaaagt ggaccaaatg gtcaaaattt acattaaaac aacaaaaata gaagacgaaa
    53221 catgtaattt ttggcagtta tagaaaattt aaaagcataa caccaaaaat aaaagggcta
    53281 aaaattaaag ccctttataa ttaataaatt aaatgtgtta cttttgcatc ataataaaca
    53341 ttttgtggtt acaaattatt gagatcaagc acatctcgca tatcaaaaag accactttcc
    53401 ttaccactca accacaaagc cgatcttacc gcaccgttag caaatgtcat gcggctggac
    53461 gccttatggg tgatctccag acgctcgcca atatcggcaa acatcgcggt atgttcgcca
    53521 acgatgtcac ctgcacgcac ggtggcaaaa ccaatggtac caggcacacg ttcaccggta
    53581 tggccttcac gactgtagac cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc
    53641 gcctctccca ttgccagtgc ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct
    53701 tcaataattt cgatatcggt gtagtcaccc atcactttgg ctgctttctc cagcagctta
    53761 agcatgacgt taacgccaac gctaaaattg gcggcaaaga caatcgcaat atcggcggcg
    53821 gcgtcacgaa ttgcctgttt accggcttcg tcaaaccccg tagtgccgat caccatccct
    53881 ttgccatgct gccgacaaaa agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg
    53941 ataaacacat caaaatcatc ttttatcgca tcaagactgc tttgcacggt aacgcctgtt
    54001 ttccctgcac cagccagctc accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc
    54061 agcgccgcgc ccagctgcac gccttctaat gccagtgccg cctgaatcaa ctggcggccc
    54121 atacgccccc cggctcccgc gatggcaacg cggatgtttg catcatgcat agctattctc
    54181 ttttgttaat ttgcatagac cgttttcaga gtaaccagcg catcgctacg ccgccagcca
    54241 aaaacgctga taattagaaa ttaatgataa acagggaggg atatcagtga aaggcatgag
    54301 tcgatgaatg actgcatgcc gataacatgt gacaggttac ggcaccagag ccagcacctc
    54361 agccacccac tgttggaagc cttttacatt cagatccaat gccacctgta cattggctgg
    54421 tttgcccagg caaccgtcga tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt
    54481 ttctaccgcg acaaaacacg gcttgagggt gaataagtcc gggcgcacca gccaggcgat
    54541 ggcgcagaga tcgtgcattc gcagaccgct ttgcatactg ccgctacggt agtggctaaa
    54601 cagggcgtgg agcatttttc cggtgcgatt caactccggt aaagtagcga gatagtcagg
    54661 ggttaatatt gcctgattgg tgacatccag accgcacatg acaatttcga tgccgctacg
    54721 gaagacacag gcagccgctt ctggatcggc agcgatatta aactcggcat ttggtgtaca
    54781 gttgccgcga ccagcagaac cgcccatgat caccagacga cgaatatacg gtttacattc
    54841 cgggcattgt aaaagtaaca gcgcaatatt ggttaacggg ccgatggcca ccagggtaac
    54901 gggctctggt gcacgcatca gggcatcccg aatcgccaga aacgccggta tcccgagtgg
    54961 ctttcggttg tgctcaacaa agtcgtagcc agccattccc gattcgccgt gcacagatgc
    55021 tgcatcacgc ggtgcgcgta ccagcggcac agcggctccc tgggcgagcg gaatctccgc
    55081 attccagaaa tgcagcagtt gcagggcatt gcgggtagtt ttctcaaccg agacattacc
    55141 cgcgacggtg gtcatcagtt gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc
    55201 gacggcatcg tcaatgccgg ggtcagtatc gaggaagata ggtaaacgca tgttttcttc
    55261 ataaaaaatg ccggtaacaa gaccggcatt ttcgcataac ttaggctgct aatgacttaa
    55321 tcgacttcac gaatatcgac acgcagctct ttcggcactt cgaaaacaat attttcttca
    55381 cggccttcca gcggaatggc ttcaccacca cccaactgct gcaaacgtgc caccacattc
    55441 tgcaccagaa tatccggagc cgatgcgccc gcagtcacgc cgacgcattt aacctctttc
    55501 acccactctt cctggatatc tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc
    55561 tgggccagct ccgccagacg gttggagttg gaggagtttt tcgaaccgac caccaacaca
    55621 acttccgcct gttctgccag ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag
    55681 atgtcatctt tgcgcggacc gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca
    55741 tcagacgtat catccaccga cagcgtggtt tgggtcataa aggagagctt ctcttcgttt
    55801 ttgaccgtca gtttccacac atcgtctggc gattcgacca gatacattcc cccttcgggg
    55861 ttgctgtact gccccatcgt cccttccact tccgggtgcc cggcgtggcc gatgagaata
    55921 gattcttcgc cacggcgact ggcgcgggca acttccatat gcactttggt caccagcgga
    55981 caggtggcat caaacaccgt caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa
    56041 acaccgtgtg cggagaaaat caggatcgcg ccgtccggca cttcgctaat ctgctcaata
    56101 aagatagccc cacgctcgcg caagctatcg accacgtagc ggttatgcac cacttcgtga
    56161 cggacatata tcggtgcgcc gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg
    56221 tctaccccgg cacaaaaacc acgcgggttg gccaacagga tctgcatgtt acgcctccag
    56281 tgccggatcg atttccagca cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg
    56341 gttgaaatca acggtaatgg agtcgccgtt aatttcgcgg atcacaccag gcatctcact
    56401 gccatccatt gcggtaaaaa gcatgattgc gccaatttct ggctcgcctg catccataaa
    56461 ttcacggcgg gagaagtact gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg
    56521 ctccagcgag aaggtggttt tgtcgcccac tttcagcccc agcaggtgtt gctccagccc
    56581 ttcagaaaga gaagcatcgc ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga
    56641 ctcggcggtg gtgccatcgt caagttttag cgtgaagtgc accaggacgg cgctattgct
    56701 ctgtacagat tcagacatgc aggtttgctc ttatttttga ggatttttgc cggatgcggc
    56761 ctacgatttc gtagcaatct gtaggccgca tccggcaggg tttattattg ttttttcgct
    56821 ttggaaggta aaaacccttc cagcacgatc agtgccgcac cgacacaaat ggcagtatcg
    56881 gcaaggttga aggtggcgaa gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg
    56941 aagccgtgcc acaggcggtc gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa
    57001 gcgatattgt ttagcttctg cgtggccttc gagcgataca tcatcactgc caggagcacg
    57061 ctaataccaa tcgcaatacc ggcaaagaac caacgctgcc agccgccgct atcggcaagg
    57121 aaactaaacg ccgcgccata gttacgcgca taatgcagat taagcgacgg gaacagcggg
    57181 accgtatccc ccagagcaaa gttctggagg atcaggtatt tgctgcccag atcgataatc
    57241 agcacgacta ccaccagcca cagccagcgt agccctgttg aacagatcga ttgactcatc
    57301 aggcaaactt acgtttttca ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt
    57361 ctgcgtgttc cgccaccttg ccgacatcct gggtgtagtg ccagcagcgt gggcacttct
    57421 caccttcggc tttactcaac gcgactttca gccctttgag tacttcgctc tgctgagcat
    57481 cagcaggtgc gtcgttatag tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta
    57541 attcatcgcc cagcgcggtc agtttcgccg ccagttccgg ttctgcatac aaggttactg
    57601 ccgcttccag cgagccaccc actttcttgt cggcacgcgc ttgctcaatg actttgttca
    57661 cttcgccacg cactttcaac agctcgtccc agaacgcatc gttcattgct tcactgtctg
    57721 ccagaccaaa caggccttcg taccactcac cggtgaagac gtatttttca cgttcgcccg
    57781 gcagatagcc ccacacttca tcagcggtga aggagaggat tggtgccatc cagcgcacca
    57841 gcgcttcagc gatgtgatac agcgcagtct ggcagctacg acgcgccacg ctatccgctt
    57901 tggcggtgta ctgacggtct ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc
    57961 agaagcgcat cagacgctgt accacttcgt ggaaatcgta tgcttcgtac gccttgagga
    58021 tgtcttcctg tgccgctttc gcacaaccta cggcccagcg atccagtacc accatctctt
    58081 ccggtttcac catatctttt gctggatcaa aaccattcag gtttgccagc aggaagcgcg
    58141 cggtgttacg gatacgacga tagctatcgg cagcacgttt caggatctcg tcagaaacgg
    58201 ccatttcacc ggtgtagtcg gttgatgcca cccacagacg cagaatatcc gcgcccagtt
    58261 tgttcatcac atcctgcggc gaaacggtgt tgccgatgga tttagacatc ttgcgaccct
    58321 gaccatccac ggtaaagccg tgggtcagta cctgacgata cggcgctttg cctttcatcg
    58381 cggtggagat cattagggaa gacatgaacc aaccacggtg ctggtcagaa ccttccagat
    58441 acatgtccgc tgcgtgaccg gcaaattccg gacgcacgtc aacaacagaa gagtgggtag
    58501 atccggagtc aaaccatacg tcgagagtat ccggcacttt cacatactgg tcagcttcgt
    58561 cgccgaggat ctctttcgca tcgagatccc accacgcctg gatgccgtca acttcaacgc
    58621 gtttagccac ttcttccatc agttcgaggg tacgcggatg cagctcttcc gtgtctttgt
    58681 gcacgaacag tgacatcggt acgccccagg tgcgctgacg ggagatacac cagtcaggac
    58741 ggttagcaac catcgactcg atacgcgcct ggccccagtc cgggatccac tgcacgcctt
    58801 tgatctcttt cagtgactgc gcacgcagac ctttctgatc catgctgacg aaccactgcg
    58861 gtgtcgcgcg gaagatgatc ggcgttttgt gacgccagca gcacggatag ctgtgctgca
    58921 ttttctcaac gtgcagcagc gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg
    58981 ctttgaagac gttcacgcca tccagcgtcg gataagtgcc cggcagataa gtgccgtccg
    59041 ggccaaccgg gttagcggtt tccaggccgt atttctgacc gatcacatag tcgtccgggc
    59101 cgtggccagg cgcggtgtga acggcaccgg taccggcatc cagggtaacg tgatcgccga
    59161 ggattgccgg aacgtcgaag cccataaacg gatgggtaaa gcgcagcagc tcaagctctg
    59221 caccttttac cgtgccgaga atggtgtaat cggtcacgcc gatacgctgc attacgcttt
    59281 caaccagatc tttcgccaga atcgcggcct gaccgtcgat ctgcaccagc gcatagtcga
    59341 aatcaggtgc aatagagatt gcgcggttcg caggcagagt ccacggcgta gtggtccaga
    59401 tcaccagcga gattgggccg ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct
    59461 gatcgaccgc ctggaaagcg acgtcaatgg acggagaagt tttgtcgtaa tactcaactt
    59521 ccgcttctgc cagtgcagaa cggcagtcta cgcaccagtg caccggcttc gcgcctttgt
    59581 gcaggtgacc attgccgatg attttgccca gcgcgcggat gatgttggct tcagttttga
    59641 agtccatagt cagatacggg tgcgaccagt cgcccagcac gcccagacgg ataaagtctt
    59701 tgcgttgacc gtcaacctgg gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg
    59761 cggtgaattt ctcacccggc ttaccgtatt cttgctctac tttcagctcg atcggcagac
    59821 cgtggcagtc ccagccaggc acatacggcg agtcataacc ggaaagccct ttggacttca
    59881 cgataatgtc tttcagaatc ttgttaaccg agtgaccaat atgaatgctg ccattcgcat
    59941 aaggagggcc atcatgcaga atgaaggttt ttttgccttt tttagccgca cggatgatgc
    60001 cgtacagatc atcatcagtc caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc
    60061 cacgcatcgg gaaccctgtt tccggcaaat tcagggttga tttatagtca ctcatcagat
    60121 tctcggttcc gtgtttcggt ttgattacat aacaggctta agccggtttt gttagcccaa
    60181 aaaattcgcg ggcggttaat tcatcacgcg caatctgcgc tttcagttcg tccagcgacg
    60241 caaatcgctg ctcattgcgt atttttttac gcagcactac ttgtatatgg cgaccgtaaa
    60301 ggtccattgc aacatctaac aaatgcactt ccagctgctg gcgaataccg gcaaccgttg
    60361 ggcgtgttcc gatgtttgcc acgccgggta acggcttttc accgaggccc agcacttcta
    60421 ccgcataaac ccctttcacc ggggaaacct gacggcgtag cggtacattc gccgtcggga
    60481 aacctatagt gcgccctaat tcatcaccgt ggactacacg cccggagatg gcaaacgggt
    60541 gccccagtaa actttctgcc agagccagat tgtcatcagc cagcgcctga cgcacggcgg
    60601 tgctgctgat acgcacacca ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt
    60661 attccatgcc agctttctgt aataacaaga aatcgccttc acgaccagcg ccaaagcgga
    60721 aatcatcacc tacggcaaga aattttacgc gcaaatgctt caccaaaaga tcgctgatga
    60781 aattttgcgc ggttaacgcc gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa
    60841 cgccacactc tgcaaggtaa cgcagttttt cccgcagccg ggtcagtctt gccggggctt
    60901 tatcggtagc aaacagttcc agtggttgag gttcaaaaag catcaccatc accggtaagt
    60961 tgcgcttgcg cccttcttcc tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc
    61021 cgtcgaaatt accaatagtc agcacacacc cttcttgcgg ggcctggctg agattatgta
    61081 tgccgcgtat cagcttcatg tctggctcaa aacagtgaaa atcgtccgag tataccttgt
    61141 acagcggtca aggttaaccg gcgattgagt accgaaatca gaaagaagtg gcttttcatt
    61201 gccatggcgc aaatcacggg aagaaactga ccgcctgctg caatttttat cgcggaaaag
    61261 ctgtattcac accccgcaag ctggtagaat cctgcgccat cactacgtaa cgagtgccgg
    61321 cacattaacg gcgcttattt gcacaaatcc attgacaaaa gaaggctaaa agggcatatt
    61381 cctcggcctt tgaattgtcc atatagaaca catttgggag ttggaccttg gctaatatca
    61441 aatcagctaa gaagcgcgcc attcagtctg aaaaggctcg taagcacaac gcaagccgtc
    61501 gctctatgat gcgtactttc atcaagaaag tatacgcagc tatcgaagct ggcgacaaag
    61561 ctgctgcaca gaaagcattt aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag
    61621 gtctgatcca caaaaacaaa gctgcacgtc ataaggctaa cctgactgca cagatcaaca
    61681 aactggctta atcgccaatt tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt
    61741 ttatgcctgc tgcttttgtg atgcgtgaga gaacacactg ccggttgcca gatagtcaaa
    61801 agagacatgc cgttgtacta tctgaactct tcgtggctta agaatcgtag aaccatcggc
    61861 tgatacattc tgaacaagaa tatctactca atctccagac tcccccacaa agaatatgga
    61921 tattgtgata cacattgagg tattaaactt aagaccactc tatttttgaa ttaaattcct
    61981 atatacttta accattcata aatatctcct cattagacct t
//
LOCUS       DADLZM010000007        60685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLZM010000007 DADLZM010000000
VERSION     DADLZM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.007"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..320
                     /locus_tag="JNY73_000452"
     CDS             <1..320
                     /locus_tag="JNY73_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8046580.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="JNY73_000453"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="JNY73_000453"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY8046581.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="JNY73_000454"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="JNY73_000454"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY8046582.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVGLA"
     gene            complement(2164..3066)
                     /locus_tag="JNY73_000455"
     CDS             complement(2164..3066)
                     /locus_tag="JNY73_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY8046583.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="JNY73_000456"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="JNY73_000456"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY8046584.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="JNY73_000457"
                     /pseudo
     CDS             complement(3724..4953)
                     /locus_tag="JNY73_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(5017..5457)
                     /locus_tag="JNY73_000458"
     CDS             complement(5017..5457)
                     /locus_tag="JNY73_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8046585.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="JNY73_000459"
     CDS             complement(5562..6332)
                     /locus_tag="JNY73_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8046586.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="JNY73_000460"
     CDS             complement(6329..7255)
                     /locus_tag="JNY73_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8046587.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="JNY73_000461"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="JNY73_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY8046588.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNY73_000462"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNY73_000462"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY8046589.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /locus_tag="JNY73_000463"
     CDS             8809..11199
                     /locus_tag="JNY73_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY8046590.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNY73_000464"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNY73_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="JNY73_000465"
     CDS             12961..13308
                     /locus_tag="JNY73_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY8046591.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="JNY73_000466"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="JNY73_000466"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY8046592.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQQS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="JNY73_000467"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="JNY73_000467"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY8046593.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="JNY73_000468"
     CDS             complement(15128..15490)
                     /locus_tag="JNY73_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY8046594.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNY73_000469"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNY73_000469"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY8046595.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIINRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="JNY73_000470"
     CDS             18618..20471
                     /locus_tag="JNY73_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY8046596.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="JNY73_000471"
     CDS             complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="JNY73_000471"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY8046597.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPNPNPKAKKK"
     gene            complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="JNY73_000472"
     CDS             complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="JNY73_000472"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY8046598.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="JNY73_000473"
     CDS             complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="JNY73_000473"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY8046599.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="JNY73_000474"
     CDS             complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="JNY73_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY8046600.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28345..29718
                     /gene="aroP"
                     /locus_tag="JNY73_000475"
     CDS             28345..29718
                     /gene="aroP"
                     /locus_tag="JNY73_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY8046601.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="JNY73_000476"
     CDS             complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="JNY73_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY8046602.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="JNY73_000477"
     CDS             complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="JNY73_000477"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY8046603.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31251..32144
                     /gene="nadC"
                     /locus_tag="JNY73_000478"
     CDS             31251..32144
                     /gene="nadC"
                     /locus_tag="JNY73_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY8046604.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32347..32787
                     /gene="ppdD"
                     /locus_tag="JNY73_000479"
     CDS             32347..32787
                     /gene="ppdD"
                     /locus_tag="JNY73_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY8046605.1"
                     /translation="MDKQHGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32797..34182
                     /gene="gspE"
                     /locus_tag="JNY73_000480"
     CDS             32797..34182
                     /gene="gspE"
                     /locus_tag="JNY73_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY8046606.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34172..35374
                     /gene="hofC"
                     /locus_tag="JNY73_000481"
     CDS             34172..35374
                     /gene="hofC"
                     /locus_tag="JNY73_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY8046607.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="JNY73_000482"
     CDS             complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="JNY73_000482"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY8046608.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            36677..37297
                     /gene="coaE"
                     /locus_tag="JNY73_000483"
     CDS             36677..37297
                     /gene="coaE"
                     /locus_tag="JNY73_000483"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY8046609.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37297..38040
                     /gene="zapD"
                     /locus_tag="JNY73_000484"
     CDS             37297..38040
                     /gene="zapD"
                     /locus_tag="JNY73_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY8046610.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38050..38247
                     /gene="yacG"
                     /locus_tag="JNY73_000485"
     CDS             38050..38247
                     /gene="yacG"
                     /locus_tag="JNY73_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY8046611.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="JNY73_000486"
     CDS             complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="JNY73_000486"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY8046612.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38796..41501)
                     /gene="secA"
                     /locus_tag="JNY73_000487"
     CDS             complement(38796..41501)
                     /gene="secA"
                     /locus_tag="JNY73_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY8046613.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41563..42075)
                     /gene="secM"
                     /locus_tag="JNY73_000488"
     CDS             complement(41563..42075)
                     /gene="secM"
                     /locus_tag="JNY73_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY8046614.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="JNY73_000489"
     CDS             complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="JNY73_000489"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY8046615.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVCVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="JNY73_000490"
     CDS             complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="JNY73_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY8046616.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="JNY73_000491"
     CDS             complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="JNY73_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY8046617.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="JNY73_000492"
     CDS             complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="JNY73_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY8046618.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46627..47547)
                     /locus_tag="JNY73_000493"
     CDS             complement(46627..47547)
                     /locus_tag="JNY73_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY8046619.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47540..49015)
                     /gene="murC"
                     /locus_tag="JNY73_000494"
     CDS             complement(47540..49015)
                     /gene="murC"
                     /locus_tag="JNY73_000494"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY8046620.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49069..50136)
                     /gene="murG"
                     /locus_tag="JNY73_000495"
     CDS             complement(49069..50136)
                     /gene="murG"
                     /locus_tag="JNY73_000495"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY8046621.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="JNY73_000496"
     CDS             complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="JNY73_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY8046622.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51377..52693)
                     /gene="murD"
                     /locus_tag="JNY73_000497"
     CDS             complement(51377..52693)
                     /gene="murD"
                     /locus_tag="JNY73_000497"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY8046623.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="JNY73_000498"
     CDS             complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="JNY73_000498"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY8046624.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53772..55130)
                     /gene="murF"
                     /locus_tag="JNY73_000499"
     CDS             complement(53772..55130)
                     /gene="murF"
                     /locus_tag="JNY73_000499"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY8046625.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55127..56614)
                     /gene="murE"
                     /locus_tag="JNY73_000500"
     CDS             complement(55127..56614)
                     /gene="murE"
                     /locus_tag="JNY73_000500"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY8046626.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56601..58367)
                     /locus_tag="JNY73_000501"
     CDS             complement(56601..58367)
                     /locus_tag="JNY73_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY8046627.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="JNY73_000502"
     CDS             complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="JNY73_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY8046628.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="JNY73_000503"
     CDS             complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="JNY73_000503"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY8046629.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="JNY73_000504"
     CDS             complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="JNY73_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY8046630.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCAIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14537 a        16811 c        14921 g        14416 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcgg gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggctccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc taggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatac cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgaggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccatgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcaatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gcagtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttaa taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cacgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcgggttcgg
    20581 gttcggcagg tcggtaatgc taccttcgaa cacttctgcc gccaggccca cagactcgtg
    20641 cagagtcggg tgcgcgtgga tggtcagtgc gatgtcttca gcatcacaac ccatttcgat
    20701 tgccagcccg atttcaccca gcagctcgcc gccgttggta ccaacaatcg caccaccgat
    20761 cacgcggtga gattctttgt cgaaaatcag cttggtcata ccgtctgcgc agtcagaagc
    20821 gatagcacga ccagaagcag cccacgggaa ggtggcggtt tcatagctga tgcctttctc
    20881 tttcgcttct ttctcagtca gacctaccca tgcaacttct ggttcggtat aggcgatgga
    20941 cgggataact ttcggatcga agtagtgttt cttaccggcg ataacttcag cggcaacgtg
    21001 accttcgtga acacctttgt gtgccagcat cggctgaccg acgatatcgc cgatagcaaa
    21061 gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg atgaaaccac ggtcgtcaac
    21121 ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac caatcgctac
    21181 cagcacggcg tcgtaacgct gcggttcagc tggtgctttt ttgccttcca tcgtcacata
    21241 aataccgtct tctttcgctt caacggcggt aactttggtt tccagcatca ggttgaattt
    21301 cttgctgata cgcttggtga agactttaac gatgtctttg tcagctgccg ggataacctg
    21361 gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtaaacgg tgcccatttc
    21421 cagaccgatg ataccgccac ccattaccag caggcgttct ggcacttctt tcagttccag
    21481 cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga ataaacggca gttggatcgg
    21541 gcgagaaccc gctgcaatga tcgcattgtc gaagttgatc acggttttgc cgttctcacc
    21601 ttcaacttcc agggtgttag ccccggtgaa tttacccaga ccgttgacca ctttgacttt
    21661 gcggcctttc gccataccag ccagaccacc ggtcagctga ttgatcactt tctctttcca
    21721 ggtacgaatc ttgtcaatat cggttttcgg ttcgccgaag acgataccgt gttcagccag
    21781 cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag ggatacagcc
    21841 gacgttcagg caaacaccgc caagggtgtt gtaacgttct acgattacgg tttccagacc
    21901 taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa gtaccacgac
    21961 ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg cggtcatacc
    22021 cgtcgtcttt caggccgcag cggcgttagc ttcacgccct caccccagtc acttacttat
    22081 gtaagctcct ggggattctg gcgcttgcta ccttgctgca cccagaaatc catagggtat
    22141 gtgaagtaat accctaacca ccaccgggtc gttctatccg tcggtatttt acaaaattgt
    22201 taacaatttt taaacaacaa acggcaaccg atttgtctat tcgctaataa cctcaataca
    22261 ttcatgagat taccagaaaa aagccggccg ttgggccggc tcttttactt acatcaccag
    22321 acggcgaatg tcagacagcg tgttgttaat gatggtaatg aaacgggcac catcagcacc
    22381 gtcgatcacg cggtggtcga aggagagaga aatcggcagc atcagacgcg gcacgaactc
    22441 tttaccattc cacaccggct ccatcgcgga cttggaaacg ccgaggatag ccacttccgg
    22501 cgcgttcaca atcggcgcga agtgggtagt acccaggccg ccgatgctgg agatggtgaa
    22561 gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct tagaaatagt
    22621 catcagctcg cgagacagct cgatgatgcc tttcttgttg acgtctttga ataccggaac
    22681 aaccagaccg ttcggggtat ccaccgccac accgatgttg atgtatttct tcagggtcag
    22741 acgctgaccg tcttccgaca gcgaactatt gaagcgaggc atctgctcaa gagctgcagc
    22801 aacggctttc atgatgaaga caaccggggt gatcttcaca tccagcttac gtttcgccgc
    22861 ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg
    22921 ggtaacatgc gggatcatca cccagttacg gctcaggtta gcaccagaaa ttttctggat
    22981 acggcccagt tccacttctt cgatttcgcc aaacttgctg aagtccacct tcggccacgg
    23041 cagcatgcca gggataccac cgccagtcgc cgccggagct gcttctgcac gtttgatagc
    23101 ttctttcacg taagcctgaa cgtcttcgcg caggatacga cctttacggc cagtgccctt
    23161 cactttcgcc aggttaacgc caaactcgcg tgccagacgg cggatcagcg gggtcgcgtg
    23221 aacgtaagcg tcgttttcag caaattcaga tttgccttcc gctttcgcag ccggagctgc
    23281 tgccggagct tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc
    23341 tgccgcaggc gctgcgcctt caacttcgaa gatcataatc agcgaaccag ttttcacttt
    23401 atcgccaacg ttgactttca gttccttcac gacgcctgca aacggagccg gaacttccat
    23461 agaagcttta tcgccttcta cggtaatcag tgactgttca gcggcaactt tgtcgcccac
    23521 tttcaccatc acttcggtca cttcaacttc gtcaccgccg atatccggaa cgttaacttc
    23581 tttcacgcca gctgctggtg cgggggtcgc tgccggagtc gcttcctgtt tagccgccgg
    23641 agctgccgcg cctgcttcac ccgcgacttc gaagaccata atcagcgagc cggtagacac
    23701 tttgtcaccc acgttcactt tgatctcttt cacggtacct gcaaacggag ccggaacttc
    23761 catagaagcc ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc
    23821 aactttcacc aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac
    23881 gtcttttgcc gccgcagccg cgggtgctgc tgccggagct gcttctttct tctcttctgc
    23941 ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc
    24001 ggtctgggtt ttatcgccaa cagagacttt gatctcttta acgatacccg cctgcggaga
    24061 tggaacttcc atagaggctt tgtcgccttc tacggtgatc agcgactgtt cggcttcaac
    24121 tttgtcgccc actttgacca ggatctcggt gatttcaact tcatcagccc cgatgtccgg
    24181 tactttgatt tcgatagcca ttattctttt acctcttacg ccagacgcgg gttaacttta
    24241 tctgcatcga tgttgaattt ggcgattgcg tcagcaacca ctttcttatc gatttcgcca
    24301 cgtttagcca gttcgcccag cgccgcaacc acgacataag aagcatcaac ttcgaagtgg
    24361 tgacgcaggt tctcacggct gtcggaacga ccgaagccat cagtacccag tacgcgatag
    24421 tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca gtttcatata gtcggtagat
    24481 gccactgccg gcgcgtcgtt catcacctga gcgatatacg gaacgcgcgg agtttccagc
    24541 ggatgcagca tgttccagcg ttcacaatcc tgaccatcac gcgccagctc ggtgaaggag
    24601 gtcacgctat aaacgtcaga acctacgccg taatctttcg ccaggatctc tgccgcttca
    24661 cggacgtgac gcaggatgga accggagccc agcagctgaa ctttaccttt gctaccttca
    24721 atggtttcga gtttgtagat acctttacgg ataccttcct cagcaccttc cggcattgcc
    24781 ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt aaacgttctc ttgtttttca
    24841 ccgtacatac gctccagacc gtcatgcata atgacagcaa cttcgtaagc gtaagccggg
    24901 tcgtaagaga tacagttcgg gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc
    24961 tgcagacctt caccgttcag ggtggtacga ccggaagtac cgccgatcag gaagccacgc
    25021 gcttgctggt ctccagccgc ccagcacaga tcgccgatac gctggaagcc gaacatcgag
    25081 taatagatgt agaatgggat catcggcaga ttgttcgtgc tgtaagaggt cgccgctgcc
    25141 agccaggaac accctgcgcc cagctcgttg atcccttcct gcagaatctg acctttctcg
    25201 tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg
    25261 ctgtaaatac caatctgacg gaacagacct tccataccga aagtacgcgc ttcgtcggcg
    25321 atgatcggta ccagacgatc tttgatcgac ttgttcttca gcatcacgtt cagagcacga
    25381 acaaaagcga tagtggtaga gagctctttg ctctgctctt ccagcagcgc gccgaagtct
    25441 tgcaggctcg gcagctcaag cttctcggtg aagttcggct gacggcttgg cagataaccg
    25501 tgcagtttct gacgctgagc gtgcagatag gtatgctctt cagaaccttc cgggaaggtg
    25561 atgtacggca gtttttcgat atctgcatca gacaccggca cattgaaacg gtcgcggatg
    25621 tgacgcacgc cgtccatgtt cattttctta acctggtgcg cgatgttttt accttcagcc
    25681 gcgtcgccca tgccgtaacc tttaatggta tgagcaagga ttactgtcgc tttgcctttg
    25741 gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc accacggttc
    25801 agtgcccaga tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc aggatattta
    25861 ccgaagaagt gttcacgaac gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg
    25921 tcaacggttt cgttcatcag ctggatcagt ttaccgctgg tatctttacg cagcagttca
    25981 tcccaacggc taccccacat cactttgatc acgttccagc cagcaccttc gaagatgcct
    26041 tccagttcgt tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta
    26101 cagttgataa cgaaaaccag gttatccagt ttttcacggg tagcgatggt gatcgcacct
    26161 ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag
    26221 gtatctttca ggccacggtg ttccagatat ttcaggaatt tagcctggta aatagcacca
    26281 atcggaccca gacccataga tacggtcggg aactgccaga attccggcat cagtttcggg
    26341 tgcggatagg aagagaggcc attgccgtga acttcctgac ggaagttatc cagctgctcc
    26401 tgagtcagac gaccttccag gaaagcacgg gcgtaaacgc ccggggagat gtggccctgg
    26461 aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac
    26521 acatcataaa tggttgcgga agactggaag gacgccatgt ggccgcccag ttcgaggtct
    26581 tttttcgacg cacgcagcac cgtcatgatg gcgttccagc ggatagctga acgaatacgg
    26641 cgttccagtt ccagattacc cggatactcc ggttgttctt caacggggat ggtgttgatg
    26701 tagttgctga tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc ttcagcaagc
    26761 agttggtcga tcagatactg agcacgctca acaccttctt cacggatgac cgattcgatc
    26821 gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg
    26881 ggttattcct tatctatcta ataacgttga gttttctgga acctgtccca ttgaactctc
    26941 gccagaaagc tcaataagac aggttctacg tttagttacc gcgcttttat atgcgcttga
    27001 tttacaacat cttctggata atttttacca gaaaaatcac taattctttc gttgctccag
    27061 acgacgcaga gaacgctcac ggcggctctc ttcacggctt ctgtcgagca aaatttcttc
    27121 gataaaggcc agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat
    27181 cgcttcaaat atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata
    27241 gagcaattcg aagttctggc ggacattctg ggccaacatc ggctccatac accttagcag
    27301 atgaagcaga accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc
    27361 gtttgattcc gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc
    27421 acggatgcgt tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc
    27481 ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt cggagagcag
    27541 ctccaccagc ggatcgctga agctttgcca taggctgctc tggacaaaag tgccgccacc
    27601 ctggcgacga agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaaggaggg
    27661 acgggagacg tcgaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg
    27721 gagagtgcct tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg
    27781 ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg aagtcaattg
    27841 gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag tattcacctt atgtccatac
    27901 aggttttgat tgaaatcatg aaactgtgca cattttaaca acttgacata tataacgttt
    27961 caaagttgta actatgcaca aatgtagact ttacgtagga aagggatttt ttaacgatta
    28021 ttaaactata aaaatccgaa ttgaaccgat tcacttacca attttttgat ttttaagtca
    28081 ataaaaacga atttaaattc actctgcata ttgagagggg ttgaggctga gctttacaaa
    28141 cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat tcgctgccgc
    28201 ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaactt ccttgatgta
    28261 aataaatcaa tacaacaaac ggaattgcaa acttacacac gcatcactgc gtagatcaaa
    28321 aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga gcagctaaag
    28381 cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat agggacaggg
    28441 ctattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat cctgggttac
    28501 gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat ggtggtcgaa
    28561 gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag ttttgccggt
    28621 ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc agagctgact
    28681 gccgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt ttctgccgcc
    28741 gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt tggcgagatg
    28801 gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat cttcggcggc
    28861 tggctgctgt tcagtggcaa cggcggcccg caggcgagcg ttagcaacct gtgggatcaa
    28921 ggtggtttcc tgccgcacgg cttcaccgga ctggtgatga tgatggcgat tatcatgttc
    28981 tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa cccggagcaa
    29041 agtatcccga aagcaactaa ccaggttatc taccgcatcc tgattttcta tattggctcg
    29101 ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac cagtccgttt
    29161 gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa catcgtggta
    29221 ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg tatgctgttt
    29281 ggtctggcac aacaggggaa cgcgccaaaa gcgctggcgt ctgtcgataa acgcggcgta
    29341 ccggttaaca ccattctggt gtctgcgctg gttacagcat tgtgcgtatt gattaactat
    29401 cttgctccgg aatccgcatt tggcctgtta atggcactgg tggtatccgc actggtgatc
    29461 aactgggcga tgatcagtct ggcgcatatg aagttccgtc gcgccaagca ggaacaaggc
    29521 gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg cctgctgttt
    29581 atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc ggtatacctg
    29641 atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa aaccgccaaa
    29701 gccgtaaaag cgcattaatc tctctacgcc ctcacccgta cagggtgagg gcaataatct
    29761 ctacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca cgaacgaggt
    29821 tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat gcggttcacg
    29881 cgccagagag aactgcgcca gacgcgttaa tacctgatat tgcgaagtat ggaaatcacc
    29941 cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg catataccac
    30001 acccgcaaga cgaaccggca cccaatccag tacatgaagc actgcatcaa tgccggactg
    30061 taaacgatga tgcggcgtct ggtatctcgc cagccagtat tgccatgcac gcaagaacgc
    30121 ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga acagcggtgc
    30181 aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca aatactcacg
    30241 ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca tcgtggcacg
    30301 ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa gacgaacttt
    30361 acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg gaacgttgaa
    30421 caatactccc tgcaatgcgc gtaacagtaa aaaagtcaca cccatcgcaa taatggtcat
    30481 gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt caagacgatg
    30541 atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca ccagtaaggt
    30601 tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc ccaatcaaat
    30661 gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt catgttatta
    30721 gcgatatcgg gatagcgatc tatcagtgcc cgcgtaaccg ccgcaagctg ttgatactgc
    30781 gcatcggtat acgccagagt atcggtgcct tcaagctcaa tcccaataga aaaatcattg
    30841 cagcgttcac gcccctgata ctgagagact cccgcatgcc aggcacgttt atcgaaagga
    30901 acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac gcgcaaatgg
    30961 gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa taatgcgtcg
    31021 atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac cagcagggtg
    31081 ggtgtttcgt catccaggcg gcaatcgtaa tgtggtgagg gaacgcggcg cgcgccaacc
    31141 agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa tacccggttc
    31201 agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac atgccgcctc
    31261 gccgctacaa ccctgacacc cgacgtgacg agctgctgga acgcattaat ctcgatatcc
    31321 ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat gccaacaatg
    31381 acattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg atcacccgcg
    31441 agaatggcgt attttgtggc aaacgctggg ttgaagaggt gtttattcaa ctggcaggcg
    31501 acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc aatcaaccct
    31561 tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact gcgcttaatt
    31621 ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa ttactggaag
    31681 gcaccaacac gcagttgttg gatacgcgca aaaccttacc tggcctgcgt tcagctctga
    31741 aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct gatgccttcc
    31801 tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc gaaaaagcgt
    31861 cctggctgca tccggatgcg ccagtagaag tcgaagtaga gaatctggaa gaacttgatg
    31921 aggccctgaa agccggagcc gatatcatca tgctggataa cttcgaaaca gaacagatgc
    31981 gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc aacgtcactg
    32041 acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc ggcgcgctaa
    32101 ctaaacacgt acaagcactc gacctttcaa tgcgttttcg ctaattgctg acagatttat
    32161 ccgctccatc gcgggcggat aatctcccct tccccaactt tcttcgtaac gcctcgcaaa
    32221 tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc cgtttcgttt
    32281 tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa tcaaggagcg
    32341 aaacagatgg acaagcaaca cggttttaca cttatcgaac tgatggtggt tattggcatc
    32401 attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg caaagccgca
    32461 ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt gtgcgcgctg
    32521 gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc
    32581 acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac cgggcaagaa
    32641 agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc
    32701 ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat
    32761 gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca gctcacggcc
    32821 ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt tcatgttgcg
    32881 gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac caccaaacgt
    32941 attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg cacacaacag
    33001 acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct gactcgaacg
    33061 ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc ggacaatgcc
    33121 taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat
    33181 gccggagtcg cattaacagc cagattaaaa gtgctgggaa acctggatat tgcggaacat
    33241 cgcctgccgc aggacggcca attcactgtc gaactggcag gaaacaccgt ctcatttcgt
    33301 attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg
    33361 agccaagcac tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct
    33421 catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa
    33481 acggtcacgc tttatagtgc cctgcaaacg ctgaataccg ctgacattaa tatttgtagc
    33541 gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt
    33601 gccgggctca cctttcaggg tgttttgcgt gcgttattgc gccaggatcc tgacgtcatc
    33661 atgatcggag agatccgcga tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc
    33721 ggtcacctgg tgttgtccac gctacacacc aattccacca ctgaaacgct ggtacgttta
    33781 cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt aatagcccag
    33841 cgtctggtac gtaaactttg cccacattgt cgccgacagc aaggggagcc catccatatt
    33901 ccagacactg tatggccgtc gccgctgccc cactggcagg cacccggttg tgtacattgc
    33961 taccacggtt tttatggtcg taccgccttg tttgaagtcc tgcccattac accagccata
    34021 cgccagctta tttccgctaa tactgaagtt gaatcgctgg aaacgcacgc ccgacaggcg
    34081 ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt aaccaccttt
    34141 gaagagttaa tccgcgtact ggggatgccg catggcaagt aagcaactct ggcgctggca
    34201 tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga gccgtacttt
    34261 actgcttatg gcactacagc aacagatggt taccccacta agcctgaagc gaatcgccat
    34321 caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct
    34381 acttaaagcc gggttaacgc tttctgaagg gctggctctg ctggcggaac agcatcccag
    34441 taaacaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt
    34501 ttccaatgcc ttattaccct ggtcagaggc atttccgccg ctctatcagg cgatgatccg
    34561 cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa
    34621 atcccagcgt cagttgaccg acaaagtgaa atcagcgttg cattatccca tcatcatttt
    34681 agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg agtttgccgc
    34741 tatctataag accttcaaca ccccactacc ggcactaacg caggggatca tgacgctggc
    34801 agactttagt ggcgaatggg gctggctgct ggtgttgttc ggctttctgc tggcgatagc
    34861 caataagttg ctgatgcgcc gcccgacctg gcttatcgcg cggcagaaat tgctgttacg
    34921 catcccgatt atgggttcac tgatgagggg acaaaaactc acgcagatct ttacgattct
    34981 ggcgctgaca caaagtgcag gcattacttt tttacagggc gtagagagcg tcagagaaac
    35041 aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg
    35101 tcatcccatc tggctggcgc taaaaaatgc cggtgagttt agcccgctct gtttgcaatt
    35161 agtgagaaca ggagaggcat ccggctcgct ggacctcatg ttagacaacc tcgcccatca
    35221 tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg aaccggcgtt
    35281 gctgatcata acgggaggaa ttatcggtac gctggtggtg gcaatgtatc tgccaatttt
    35341 ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc cacgccagcg
    35401 ttgggagatt acaggttgtt gaagatgcgg ttttcttgtt ccagcacacg aataaacgtg
    35461 gtgcgcttgg tcagctcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg
    35521 ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag cttgacggtt
    35581 ttaccttctg ctgcgcggta ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg
    35641 gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg
    35701 ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag
    35761 gctttcgcca catcgcccgg cgtggtacag ccaccgtcgc tgacgatcat tccgcccaga
    35821 ccgtgcgccg catcggcaca ttcgattacc gcagaaagct gcggataacc gacgcctgtt
    35881 ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcagcaccg
    35941 gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg
    36001 gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc
    36061 gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt
    36121 tcgaaatccg catcagacgt gccggtagaa accatcacat gtttcagcac atcagcggaa
    36181 gaattgttga taaacgcttg ccactcttcg acagaaaagt gtttatgcac agcagtcaga
    36241 atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc catatttgcg
    36301 gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa ttgacgttcc
    36361 agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta
    36421 aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata
    36481 agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact
    36541 gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa
    36601 cgtttcatca atttcacttt cgcgcgctca tttttaacgt tttgataatc atgatgaatt
    36661 cccgggaata taaattatgg ggtatatagt tgccttaacg ggaggcattg gcagtggtaa
    36721 gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat
    36781 tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg
    36841 cgaaaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt
    36901 cgccaacccg gaagagaaaa actggcttaa cgacctgctg catccgctga ttcagcaaga
    36961 gacgcaacac cagatccagc aagcaacttc cccctatgta ctgtgggttg tgccattgct
    37021 ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga
    37081 aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgaacatg tcgaacaaat
    37141 ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg tcattgataa
    37201 taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac actatttgca
    37261 gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa
    37321 catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc
    37381 accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt
    37441 gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttaac
    37501 cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt
    37561 gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc
    37621 gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca
    37681 ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg
    37741 cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt
    37801 accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat
    37861 ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat
    37921 tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg ttttatgccg
    37981 ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc ctgttgctaa
    38041 ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggtg
    38101 tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc
    38161 ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat
    38221 gactggagcg aagagccaaa gcagtgacat ttgtctgatg cctcacgtag gcctgataag
    38281 acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta
    38341 tccaacctac agacgcttaa gcttcgcaat taccggttca ttggctggcg gaaaatcatc
    38401 ggcattaaga ccgaccagcg acatccactc accgggttgc ccttctttac cccacggcac
    38461 cccttcccaa ctttcgacca gccagaacca cagagttata tgcctgtccg ggaattcata
    38521 ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacctctt cctgaagttc
    38581 acgcaccacc gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg gaaactccag
    38641 tttattcgcc atgtgcgcat ctgctgcgcg acgtgttata aagatttcat tgttctcgtt
    38701 gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg
    38761 cagaatcctg cgccttttac ttcaactgtt agcttttatt gcaggcgacc atggcactgc
    38821 ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt gcgttcaccg
    38881 gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct aagctgctgc
    38941 atttgtgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc aacctcttca
    39001 ggcatacgta cctgaacttt gctcagcgta ctgataactt catatttcaa cgactccagc
    39061 atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc tttctgtgca
    39121 tagccacgca ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg ctctttccac
    39181 agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat ctcagcacca
    39241 accacttctt ctttacgctg atacacttcg atggactgcg ccagaatgcg ctcacgcagc
    39301 gtctcttcat gcagttctgg ttctttatcc agccactcgg caattggtaa atcgaggtcg
    39361 aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc cagcgactgt
    39421 ggcggaatgt aggcatcaat ggtggctttg aacacatctt cacgaatgct gttgatggtt
    39481 tcgctcacat cgctgacatc cagcagttcg ttacgctggg agtaaatggc gcgacgctga
    39541 tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acgactttca
    39601 actttacgct gggcgttggc aatcgcttta gtcacccacg ggtgttcaat ggcttcgcct
    39661 ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc
    39721 agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa
    39781 cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc gatgatatgc
    39841 aggccacctg cttccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca
    39901 atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct acctccgagc
    39961 acaatatctg taccacgacc cgccatgttg gtcgcgatag tcaccgcagc cggataacct
    40021 gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac attgtgctta
    40081 ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga gatagtaccc
    40141 accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat cgcctgaatt
    40201 ttttccgctt cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg
    40261 gtcggaacaa cgacggtatc cagtttgtag atggagctaa attcgaaagc ttcggtatca
    40321 gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc
    40381 gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc cacagcctgg
    40441 tgcagaccat cggaccagcg tcggccctgc atggtacgac cggtgtgttc gtcaacgatg
    40501 ataacttcac catctttaac gatgtagtcg acgtcacggg taaacagcgc atgagcgcgc
    40561 agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag agactcccct
    40621 tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc acgttcggtc
    40681 aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg gaaggtttcg
    40741 gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg tttatacatt
    40801 tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc atcgatcagg
    40861 atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg ttcttcaggg
    40921 ctgaacgcca tgttgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagtg
    40981 atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag gttgatacca
    41041 acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg
    41101 tagtcgttga cggtaactac gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc
    41161 agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta
    41221 agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac gcgcttactt
    41281 gcctcacgca ccacggcgaa agcttccggg atcagatttt ccagcacttc gcctttttcc
    41341 agacgcgcac gaaactctgc ggttttccct ttcagttctt catcggagag tttttccatc
    41401 tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag ggtgcgatcg
    41461 ttacgactac cgaaaacttt agttaacaat ttgattagca taataaaatc ccaaacgccc
    41521 cgcgttacgg agttaataaa atgaagtaaa ggtttattgt tgttaggtga ggcgttgagg
    41581 gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt ttgcttgtgg
    41641 ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt cctgggtcag
    41701 cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca aagattcttc
    41761 agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga tgacggatta ccgtgcgaat
    41821 agcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgcg tgttcgcttc
    41881 cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc
    41941 ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc
    42001 gaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt
    42061 cagtattcca ctcacgttat tgccatcccg atcgaattca aacgtattta gcgcacaaat
    42121 cttatcatta aagcctgaag tccacagcac gaatgaagat aagttgtgcg taaaagtgct
    42181 taagaaaaca gcgttcgcac cgttttttat agctcttata aaattcaaaa aaaaacgact
    42241 ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg acagtataaa
    42301 tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc
    42361 tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag cagtttgtta
    42421 ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg accacacatg
    42481 aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc aaaacgcagg
    42541 ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa gctgccgccc
    42601 aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa cgtacgcgca
    42661 cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg ctggttgctg
    42721 gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc
    42781 ttaaattcag cccacttatc gccatcttcg acacaaacag tctctttgat gcgaacaaat
    42841 tttttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac aaacggagcg
    42901 gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat gttatcgatg
    42961 cccaggcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac atcatgctcg
    43021 ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc cgggaaatct
    43081 accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg cgcagggcgt
    43141 aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt
    43201 ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc aaccgaagtg
    43261 tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa ttccagtcaa
    43321 ttcttaatca gcttgcttac gtaggaatgc tgggatatcc agataatccg gctctttcgc
    43381 agtttgcggc gcattgtcat tcactacttt agcaaccggc ttctgctcct gggtcagcgg
    43441 agccatccca tgctgctggt agcgatccat cactggctgc tgaacctgct tattggtcac
    43501 cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg ttacgcgcag
    43561 ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt tgtcggaagc
    43621 aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc
    43681 cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg atatcttcca gcagcggaga
    43741 agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca cgccagaacc
    43801 catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt ccacgttcat
    43861 caaacccgga cgagtaatca gttcggcgat accttgcaca gcgcctttca gtacatcgtt
    43921 cgctgcgcca aacgcatcca gcaaggagat accgcggccc agaactttca gcagtttgtc
    43981 gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc cctgctcggc
    44041 gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag caacggtcag
    44101 gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac cggtaccacc
    44161 acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac gcaatgcatc
    44221 gcgatcctca tcagccgcat tgcggccaac ttctggatta gcgccagcgc ccagtccttt
    44281 ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc
    44341 atcggtattt accgcgaaga attcaacacc ttcaatacgc tcgcgcacca tgtgttcaac
    44401 agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat tggtaagttc
    44461 cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg
    44521 tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct tgatccacga
    44581 gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat gtgactcttt
    44641 cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat aatccgttaa
    44701 accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc gctgagcaca
    44761 ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg ccgccaggtg
    44821 atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt cgttgaccag
    44881 gttgagcaac tcggtatagc gcggctcgat cacctctgcc agggtctgac gttgcagact
    44941 ccgcggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc caacgatgga
    45001 acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa
    45061 ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct tagtgtggcg
    45121 caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat cgacgacgca
    45181 gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca gtccggcaaa
    45241 tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat
    45301 atcgttgtga catgtgatca ggtgcacttt tgcctgcatc cgcacacccg aaagtcctac
    45361 cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca catgcagcac
    45421 acgatgctca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt ccacatcttc
    45481 ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc agctgatgtg
    45541 cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca attctgcctg
    45601 gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc cgcctttatc
    45661 cataccacgt gacgggcagc tgcccacgcc aatgatattg accataccgt cgggcagaac
    45721 ttcccctact aaagcggcaa ccttcgcggt accaatttcc agtcctacta ccagttttct
    45781 gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg agtagattcc
    45841 tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa atcaacgtag
    45901 ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag
    45961 cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc gttattcagc
    46021 gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt
    46081 gccagcatct gccccatttc gcgatagccc tgcaacactt cattagcgct gccttccggg
    46141 ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt
    46201 ccttccgcgt ctaccatgtg ttgatcattc caccgcgcaa tcggcacata ttcaaccaga
    46261 tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat ccacggcagg
    46321 cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt acccggctca
    46381 cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc
    46441 aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagctcaa cacgacccag
    46501 ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt
    46561 ccattattgc ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag agcagcctgc
    46621 gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc tcatacctgc
    46681 ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg tattggcttc
    46741 cagcagataa aactgtccat cactgtccag cataacgtca atacgtcccc atcctttgca
    46801 acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttt
    46861 cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct cataatcata
    46921 gaaggttccg gacggttgaa tacgtattga cggtaaaatt tcttcaccga gtatcgcaac
    46981 cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt gctgaaatgc
    47041 caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc ccacactgga
    47101 accttcgcgg ctcggcttaa cgataaccgg caaacccaga gcagaaattt ctgctaactg
    47161 cttatcgctc aggccttttt caaactctgc gcgggttaac gctacccacg gcgcgaccgg
    47221 taaaccggca ccttgccaca gaagtttgct gcgtagttta tctattgaaa gcgcagatgc
    47281 catcactccg ctgccggtat aaggcaagcc catcagctcg agcatgccct gcagcgtacc
    47341 atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca tcgacttcag
    47401 ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca tcaataccgc cttcacgcag
    47461 cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg aagtcccgcc
    47521 caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt
    47581 tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga acgagaatca
    47641 ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgcaggg tccggcacca
    47701 gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc
    47761 ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta tcaacctgcg
    47821 tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa
    47881 acagcattac caggttttta tccggccagc ctgcgcgcgc cgctttaatg gtggcgtcaa
    47941 cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt ttaccattca
    48001 ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc tggaagctct
    48061 ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag
    48121 ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc atcggctctt
    48181 tgtcctgacg cagcagcgta aagtgcccct gcgggccaat ctgctgataa tcttctacac
    48241 gcacgtcggc atcttcgctg aagccgtaag ttgtggtctg acgtcccaca cgcggtaaca
    48301 attcgcggat caccggatca tcaacacaca tcaccgcacg accgtaaaaa ggcaggttgt
    48361 gcagaaaatt aataaaggtc tgttttaaat tctcaaagtc gccctggtag gtatccatgt
    48421 ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg aacgatgcat
    48481 cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca tgaacccccg
    48541 ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc
    48601 tggaaaccat cgcggtagtt gtcgttttgc cgtgcgttcc tgcaatggcg atgccatgac
    48661 gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata cgcgcttcat
    48721 gagctgcgac aatttccggg ttgtcggcag aaatggcgct ggaaacaacg accacgctgg
    48781 catcacgtac gttttccggg cgatggttga aataaatcgt cgcacccaga ttcagtaact
    48841 gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct tcattggcca
    48901 gaacttcggc aataccgccc ataccggcac caccaatgcc gacaaaatgt atgtgccgaa
    48961 cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta ttcattcttt
    49021 acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt acgcccgggc
    49081 agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc
    49141 gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt tggcgacagc
    49201 atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt ccagcggtag
    49261 cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc
    49321 tgccgcggcg atttcactca cagttaaagc accggagcgg caaacgacga catccgccca
    49381 cgcatacgcc gccgccatat catcaataaa ttcagtcact ttatgctgcg gttgccccgc
    49441 ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat gccagatagt
    49501 gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc
    49561 ctgggaacca cccactacca acacacgaac cggaccttca cgtccagcca aacgttgctg
    49621 cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt ccgcattagg
    49681 gaaagcgcct ggaaacgcct gcatcacttt ggtagcaatc ttcgccagcc atttattggt
    49741 taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg accacgcggc
    49801 cagaccacct ggacctgata catagccgcc cataccgagt accacgtcag gtttgtacgc
    49861 tttcataatc gcccgcgcct gacgccaggc gttgaagata cgcagcggcg cagctatcag
    49921 tgcttttata ccttttccac gcaaaccaga gatacgaatg aaatcaattt cgatgccatg
    49981 ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc
    50041 ctgagccatt agatagtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc
    50101 tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac aaacgcctgc
    50161 gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata
    50221 atcagtaagc tcgaaccacc gtaactgatt agcggcaatg tcagaccttt ggtcggtaac
    50281 atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat gccaatagaa
    50341 caggcgagaa aaccggaaaa acggtggtca atttctaatg ctttgcggcc aatcgacatc
    50401 gcacgaaaag cgacgaagaa taccattaaa agtgctagta ccacaccgac ataacctaat
    50461 tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt
    50521 tgtaccgaat tacctaaacc ttgcccccaa agttcgccgc gaccaaacgc catcagcgat
    50581 tgcgttaact gatagccgct gccaaaggga tcttcccacg ggttccagaa tgcggtaaca
    50641 cggcggatac ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat gcccataccg
    50701 ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc
    50761 acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc
    50821 agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta
    50881 cgcaccagat agttggcgat atagcaaaac aacgacagtt ttgtcagctc cgcaggctgg
    50941 atacgcagca aaccgagatc gatccaacgc gatgcccctt taacagcgct acctactacc
    51001 agcacgatca tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag
    51061 aactccatcg gcagacgcag cgtaatgatc gccagaataa acgccaaaat cagatagaca
    51121 ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac
    51181 gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg
    51241 cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcccttt
    51301 agcgccgtgg agatccagac caagatactg aatcctggca ggcgcggcat tttcaggcga
    51361 gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt
    51421 gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagcaga accatatcgc
    51481 ccggctgaac acgcggagcc agcaagcgca tagcctgttc catagtttcg gtttgttctg
    51541 ccacttccgg gcgtagcgcc gccagctgcg cgccgtcacg accgaaacaa tacagacgta
    51601 cgttatcgcc attcaggtaa cgcgccagtg ggctaaagtc cgccgattta ccatcgccgc
    51661 ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt cagcgccgct tccgtactgc
    51721 cgacgttggt cgcttttgaa tcgttaatcc agcgcacacc gttatgctcc agcaccactt
    51781 caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggta
    51841 accctgcggc atctgccagc gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg
    51901 aaagtttcat ctctttcaca ttcagcactt tctcgccttt aacccgcagc caggtttcgc
    51961 cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg cagcgttcat
    52021 ccgcaccgcg aatcggcatt gttaaggcat catcagcatt aaccacgcaa actttcgcgt
    52081 tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa accaaacggg tagcgatcca
    52141 tatgatcttc agtcacgttc agaatggtcg ctgctaccgc ctgtaagctg gaggtggttt
    52201 ccagctggaa gctcgacagt tccagcacgt acagttcaca ctcatcatcc agtagcatca
    52261 acgcaggcag gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt
    52321 cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg
    52381 cttgtgcttc gcgacaaaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg
    52441 cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc agatctgccg
    52501 ccatcagcca ttcatcattc agactgcccg tgtggcgttc tacggcttcg ggtaatttat
    52561 ccaggctagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga
    52621 aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacaaca tttttacctt
    52681 ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga ccagcatcag
    52741 cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca gttcatagtg
    52801 gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg
    52861 caggatgaca gaaagcgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc
    52921 ctgacgtagc agtacggcga taatgcctaa cgcaccacct aacgccagcg aaccgacatc
    52981 gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagtc ctgccccgac
    53041 tatcgcggta cagacaataa ccagttcccc ggcgtgtcgc agatacggta tgtgtaaata
    53101 gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa
    53161 tacggtcggc ataattgcca gaccgtcaag accatcggtc aggtttaccg cgttgccagt
    53221 acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagttgcg gcatcacatc
    53281 tttaaagaat gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa ggtacaaagc
    53341 gaaagcaaca cccagcgcaa tgaccgacat ccagaaatat ttccaacgag cgatcaaccc
    53401 tttggtatct ttacgcacca ctttgcgata atcatcaaca aagccaataa caccgtaacc
    53461 taccagcacc accaacacgc accagacgta cggattggac gggtaagccc acagcagcac
    53521 ggagatcaca atcgctgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct
    53581 gaagtgtgat tcaggaccgt cgttacgcac tacctgacca aaggaaagtt tttgcaaatg
    53641 agcaatcatg cgcgggccca tccacaatga gatgaacagc gcggtcagca ggctgacgat
    53701 ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc
    53761 ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggca
    53821 gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta
    53881 agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc caacgccgct ggcggtgctg
    53941 atagcatggc tttgtttacc catgcttaac acgcggtcaa taccagcagc ttttgccgcc
    54001 tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc
    54061 accagcacgc ggtagcccgg catttcagcc agtacctgaa cagctgcggt cattgaaccg
    54121 acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttg gatggggaac
    54181 agacggccag gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg
    54241 cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc
    54301 aacggcagca gaacatcgac gctaccggtt ggggtttgta gggtaaattc cataccgtgc
    54361 gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc
    54421 cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc
    54481 ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca
    54541 cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgccgct
    54601 tccgggcgag tcagactcac agtccaggca atctcgccct gatggttcgc gccaagttca
    54661 ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac accgatgtcg
    54721 ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc
    54781 atctctttaa cggaggtttt gccggaggac cccgtcagag caaccacgcg cgccggaact
    54841 tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt gacgattaac
    54901 tgcggcaggt cgatgtccag cggacggcta accagtagtg cgcctgcgcc gccagctttc
    54961 gcctggtcgg caaaatcgtg ggcatcaaaa cgttcacctt tcagggcaac aaacaggcag
    55021 cccggcgtca gttttcgggt atcagtggtt acagcatcaa gggtgatatc tgcaccttgc
    55081 agttcaccgt tgagaatatc ggtaagttgg ctaagggtta cgctaatcat gcaatccccc
    55141 ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct
    55201 ggtaatcctc atggcctttg cccgcgacca gtaccacatc attctcttta gcctgcataa
    55261 cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca
    55321 ttcccgccag aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg
    55381 tcaccaccgc cacgtcagca aactcttcgg caattgcgcc catcagtgga cgcttacctt
    55441 tatcgcgatc gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg
    55501 ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg
    55561 gtttgcctgg cgcagtgaac acttccatac gtccgcaaac cggttgcaga cgcgcggcgg
    55621 ttttcagcag atcagccagt ggatagccca gcgccagcag tgttgccagc gcgagcaaca
    55681 ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccagcttg
    55741 aactaaagcg aatggtcgcg ccgctgtcgt gatagttcac ttcggtcgct ttcaaccagc
    55801 gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaaccgcg tccggcagtt
    55861 ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga ccgcaatgat
    55921 gttcagaata aagcagccat ttcgcagctt cgtagtgttc catatcacca tgataatcaa
    55981 ggtgatcgcg gcttaagttg gtaaagaccg acgccggaaa tttcaatgcc gccacacggt
    56041 gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca
    56101 gccccgccag ctcatgctga acatcaactg ccgaaccggt agtattttct gtcgggatca
    56161 ctttccccag cagaccatta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt
    56221 ggctccactg cgccagaagc tgggtcgtcg tggttttgcc gttggtgccc gttacgccta
    56281 cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct
    56341 cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg
    56401 tcgcctcatc tttcgcctct gcaataatgg cagccacacc ttgcgctatc gcctgcggga
    56461 tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg cccgccgcag
    56521 ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct ggcacccacg
    56581 gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg attaatcaca
    56641 aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt acgcaatacg
    56701 ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc
    56761 tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct
    56821 gcggtataag caatatattt attgatgtag cgaccgtccg gcccgacctt tttcgcggta
    56881 ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc gccgcctggt
    56941 agcgccacgc tttccatcat atgcaccacg gtgcgaacaa tggattccgg gaagacacgt
    57001 tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat gccgtagctg
    57061 ccgatagttg cgtagactcg cgctaactgt aacggtgtta ccattagccc gtagccgaaa
    57121 gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa gccactgcgt
    57181 tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta agtatctact
    57241 aacgctgagg acggcatcgc taacgccagc ttggaaacac cgacgttact cgacttctgt
    57301 aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta
    57361 attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg
    57421 gtcatcacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt gatggtacgg
    57481 ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga cgggctgtta
    57541 gccatcgcca gcacttcacc tgtgttgaca tccaccagca cggcgctacc agattcagcc
    57601 ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag gcgttcatca
    57661 atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg
    57721 cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg
    57781 aaactcttct caacgccctc aatcccttga ctatcgacgt tagtaaagcc gatgaggtga
    57841 gcagtcactt cgccggacgg atagtaacgg cgagactctt cacgcagatg aatccccggc
    57901 agtttcagtt ttttgatgta gtccgccatg tcagagttta cctgacgcgc cagataaata
    57961 aagcgccctt tcgggttggc gttaatgcgg gcagaaagct gatccagcgg aatattgagc
    58021 gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc atgcacttct
    58081 ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg
    58141 tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc
    58201 tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa
    58261 gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat aaagttggca
    58321 tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat
    58381 ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt
    58441 tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg
    58501 ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg ctgagcggtc
    58561 agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg
    58621 aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat
    58681 gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg
    58741 ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca cgagggttct
    58801 cagccacctc ttcttcgccc ggcattaact tgcctagtgc tcgcagctga cggccaccca
    58861 gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga ccgcggctgt
    58921 tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa
    58981 gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca
    59041 gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact
    59101 tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg
    59161 gctgttcgcg gttacgctcg acaatagcgc gggcaatgcg tttggcaaaa cgctcttcac
    59221 cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg
    59281 ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa
    59341 agccgcgttc agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt
    59401 cgatcttgcc aataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt
    59461 ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc
    59521 gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac
    59581 caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa
    59641 cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag
    59701 tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat atcttccttg
    59761 acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc
    59821 atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct
    59881 gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca
    59941 accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg
    60001 gggtaaagca gcaggcacgg gtgatgaatg tcaatggcgc aaaccatttg accggcagcg
    60061 ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt gctgtcgaga
    60121 ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac
    60181 aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg
    60241 cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc
    60301 aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca
    60361 ctgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga
    60421 gcggcatttt aggacatatc ttccccggtt aacagtcctt gttacgtctg tgtggcgctt
    60481 gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg ttaaatcggc aattcatcgc
    60541 cgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc
    60601 agctggtgag ttaattaacg ggcgggttta aaaccattta tcaataatag tcaggaatag
    60661 tcttatttac tttaaggata ttgat
//
LOCUS       DADLZM010000008        59685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLZM010000008 DADLZM010000000
VERSION     DADLZM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.008"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(409..1575)
                     /locus_tag="JNY73_000505"
     CDS             complement(409..1575)
                     /locus_tag="JNY73_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HAY8046631.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(1597..2136)
                     /locus_tag="JNY73_000506"
     CDS             complement(1597..2136)
                     /locus_tag="JNY73_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046632.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(2392..2736)
                     /gene="tdcR"
                     /locus_tag="JNY73_000507"
     CDS             complement(2392..2736)
                     /gene="tdcR"
                     /locus_tag="JNY73_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY8046633.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            2925..3863
                     /gene="tdcA"
                     /locus_tag="JNY73_000508"
     CDS             2925..3863
                     /gene="tdcA"
                     /locus_tag="JNY73_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY8046634.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            3962..4951
                     /gene="tdcB"
                     /locus_tag="JNY73_000509"
     CDS             3962..4951
                     /gene="tdcB"
                     /locus_tag="JNY73_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY8046635.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            4973..6304
                     /gene="tdcC"
                     /locus_tag="JNY73_000510"
     CDS             4973..6304
                     /gene="tdcC"
                     /locus_tag="JNY73_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY8046636.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            6330..7538
                     /gene="tdcD"
                     /locus_tag="JNY73_000511"
     CDS             6330..7538
                     /gene="tdcD"
                     /locus_tag="JNY73_000511"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY8046637.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWIASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            7572..9865
                     /gene="tdcE"
                     /locus_tag="JNY73_000512"
                     /pseudo
     CDS             7572..9865
                     /gene="tdcE"
                     /locus_tag="JNY73_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            9879..10268
                     /locus_tag="JNY73_000513"
     CDS             9879..10268
                     /locus_tag="JNY73_000513"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY8046638.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            10340..11704
                     /gene="tdcG"
                     /locus_tag="JNY73_000514"
     CDS             10340..11704
                     /gene="tdcG"
                     /locus_tag="JNY73_000514"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY8046639.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCD"
     gene            11979..13256
                     /locus_tag="JNY73_000515"
     CDS             11979..13256
                     /locus_tag="JNY73_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046640.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YSVSFTLAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILR
                     RKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLV
                     YKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            13284..14576
                     /locus_tag="JNY73_000516"
     CDS             13284..14576
                     /locus_tag="JNY73_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY8046641.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14788..14952)
                     /locus_tag="JNY73_000517"
     CDS             complement(14788..14952)
                     /locus_tag="JNY73_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046642.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(14975..15676)
                     /locus_tag="JNY73_000518"
     CDS             complement(14975..15676)
                     /locus_tag="JNY73_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY8046643.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            15781..16677
                     /locus_tag="JNY73_000519"
     CDS             15781..16677
                     /locus_tag="JNY73_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8046644.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGQLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            complement(16884..17252)
                     /locus_tag="JNY73_000520"
     CDS             complement(16884..17252)
                     /locus_tag="JNY73_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY8046645.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(17545..18531)
                     /locus_tag="JNY73_000521"
     CDS             complement(17545..18531)
                     /locus_tag="JNY73_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY8046646.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(18601..19083)
                     /locus_tag="JNY73_000522"
     CDS             complement(18601..19083)
                     /locus_tag="JNY73_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY8046647.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFSFLTRTTT
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            complement(19179..19478)
                     /locus_tag="JNY73_000523"
     CDS             complement(19179..19478)
                     /locus_tag="JNY73_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046648.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(19468..19872)
                     /locus_tag="JNY73_000524"
     CDS             complement(19468..19872)
                     /locus_tag="JNY73_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046649.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(19875..20180)
                     /locus_tag="JNY73_000525"
     CDS             complement(19875..20180)
                     /locus_tag="JNY73_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY8046650.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(20218..20572)
                     /locus_tag="JNY73_000526"
                     /pseudo
     CDS             complement(20218..20572)
                     /locus_tag="JNY73_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            complement(20719..21102)
                     /gene="mzrA"
                     /locus_tag="JNY73_000527"
     CDS             complement(20719..21102)
                     /gene="mzrA"
                     /locus_tag="JNY73_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY8046651.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(21106..21768)
                     /locus_tag="JNY73_000528"
     CDS             complement(21106..21768)
                     /locus_tag="JNY73_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8046652.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(22113..22889)
                     /gene="exuR"
                     /locus_tag="JNY73_000529"
     CDS             complement(22113..22889)
                     /gene="exuR"
                     /locus_tag="JNY73_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY8046653.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            23185..23901
                     /locus_tag="JNY73_000530"
     CDS             23185..23901
                     /locus_tag="JNY73_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8046654.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            23931..24431
                     /locus_tag="JNY73_000531"
     CDS             23931..24431
                     /locus_tag="JNY73_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8046655.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            24523..27032
                     /locus_tag="JNY73_000532"
                     /pseudo
     CDS             24523..27032
                     /locus_tag="JNY73_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            27485..28117
                     /locus_tag="JNY73_000533"
     CDS             27485..28117
                     /locus_tag="JNY73_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY8046656.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(28205..29503)
                     /locus_tag="JNY73_000534"
     CDS             complement(28205..29503)
                     /locus_tag="JNY73_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8046657.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            29986..31398
                     /gene="uxaC"
                     /locus_tag="JNY73_000535"
     CDS             29986..31398
                     /gene="uxaC"
                     /locus_tag="JNY73_000535"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY8046658.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSSRGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            31413..32900
                     /locus_tag="JNY73_000536"
     CDS             31413..32900
                     /locus_tag="JNY73_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY8046659.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            32983..33534
                     /locus_tag="JNY73_000537"
     CDS             32983..33534
                     /locus_tag="JNY73_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY8046660.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(33539..34783)
                     /gene="sstT"
                     /locus_tag="JNY73_000538"
     CDS             complement(33539..34783)
                     /gene="sstT"
                     /locus_tag="JNY73_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY8046661.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(35379..36344)
                     /gene="alx"
                     /locus_tag="JNY73_000539"
     CDS             complement(35379..36344)
                     /gene="alx"
                     /locus_tag="JNY73_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY8046662.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(36615..37631)
                     /locus_tag="JNY73_000540"
     CDS             complement(36615..37631)
                     /locus_tag="JNY73_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY8046663.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(37692..38384)
                     /locus_tag="JNY73_000541"
     CDS             complement(37692..38384)
                     /locus_tag="JNY73_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY8046664.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(38461..38964)
                     /locus_tag="JNY73_000542"
     CDS             complement(38461..38964)
                     /locus_tag="JNY73_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8046665.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            39049..40185
                     /gene="rlmG"
                     /locus_tag="JNY73_000543"
     CDS             39049..40185
                     /gene="rlmG"
                     /locus_tag="JNY73_000543"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY8046666.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            40470..40784
                     /locus_tag="JNY73_000544"
     CDS             40470..40784
                     /locus_tag="JNY73_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HAY8046667.1"
                     /translation="MHLITQKALKDAAEKHPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            40781..41197
                     /gene="higA"
                     /locus_tag="JNY73_000545"
     CDS             40781..41197
                     /gene="higA"
                     /locus_tag="JNY73_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY8046668.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(41242..43260)
                     /locus_tag="JNY73_000546"
     CDS             complement(41242..43260)
                     /locus_tag="JNY73_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY8046669.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLQLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(43486..45837)
                     /gene="ygjK"
                     /locus_tag="JNY73_000547"
     CDS             complement(43486..45837)
                     /gene="ygjK"
                     /locus_tag="JNY73_000547"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY8046670.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(45854..46925)
                     /gene="ygjJ"
                     /locus_tag="JNY73_000548"
                     /pseudo
     CDS             complement(45854..46925)
                     /gene="ygjJ"
                     /locus_tag="JNY73_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(47059..48492)
                     /locus_tag="JNY73_000549"
     CDS             complement(47059..48492)
                     /locus_tag="JNY73_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8046671.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(48555..49004)
                     /locus_tag="JNY73_000550"
     CDS             complement(48555..49004)
                     /locus_tag="JNY73_000550"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY8046672.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(49001..52087)
                     /gene="ebgA"
                     /locus_tag="JNY73_000551"
     CDS             complement(49001..52087)
                     /gene="ebgA"
                     /locus_tag="JNY73_000551"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY8046673.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAYYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIINRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(52269..53252)
                     /gene="ebgR"
                     /locus_tag="JNY73_000552"
     CDS             complement(52269..53252)
                     /gene="ebgR"
                     /locus_tag="JNY73_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY8046674.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELNN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            53471..53802
                     /locus_tag="JNY73_000553"
                     /pseudo
     CDS             53471..53802
                     /locus_tag="JNY73_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(53844..55286)
                     /gene="ygjG"
                     /locus_tag="JNY73_000554"
     CDS             complement(53844..55286)
                     /gene="ygjG"
                     /locus_tag="JNY73_000554"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY8046675.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLDGFR
                     QLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEP
                     PLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            55592..57112
                     /locus_tag="JNY73_000555"
     CDS             55592..57112
                     /locus_tag="JNY73_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY8046676.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMFTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(57266..57889)
                     /locus_tag="JNY73_000556"
     CDS             complement(57266..57889)
                     /locus_tag="JNY73_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY8046677.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            58166..58912
                     /gene="nfeF"
                     /locus_tag="JNY73_000557"
                     /pseudo
     CDS             58166..58912
                     /gene="nfeF"
                     /locus_tag="JNY73_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(58966..59041)
                     /locus_tag="JNY73_000558"
     tRNA            complement(58966..59041)
                     /locus_tag="JNY73_000558"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59006..59008),aa:Ile,seq:cat)
     gene            59166..59672
                     /gene="mug"
                     /locus_tag="JNY73_000559"
     CDS             59166..59672
                     /gene="mug"
                     /locus_tag="JNY73_000559"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY8046678.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT        14814 a        14597 c        15312 g        14962 t
ORIGIN      
        1 ggtcagtttc acctgatata cgtaaaaacc cgcttcggcg ggtttttgct tttggagggg
       61 cagaaagatg aatgactgtc cacgacacta tacccaaaag aaagcggctt atcggtcagt
      121 ttcacctgat gtacgtaata aaccgttccg gcgggtttca gattgttgag tgcgctttat
      181 tcatgccgga tgctgcgtga acgccttatc ctgcctacaa aagcacgcaa attcaatata
      241 ttgcagagat ggcgtaggcc tgataagcgt agcgcatcag gcatttttgc ggttgccatc
      301 agtttcaaac ccgcgtcgat aagtttttgc ttttggtgca cagggtaaga tgaatatcta
      361 cggtattaat aatagacttt gtaagaagtt attaagtcta tattgcattt aaaaggtcgt
      421 aattatacgt atagtcctct ctggtaaaat attgattctc tgagttatat gcttcatatg
      481 tgttatattc cccgacctca aagccattgt ccgtagaatt ttcgtctttc tcgagggtat
      541 taactcttct gagctagagt cttctatgtt taaatccata gtataattaa tttgggggac
      601 gaccagataa gcattttctt tgatattttc ataatcttca atgattgtat cgatgtaatc
      661 atatattttt tttgatgaga attctttcat ctctggtaat ttaattctgt tcaagcaatt
      721 gttgattttc gatgagcttt tatatatttc ttttgttaat tcattttcta atttatctgt
      781 aagagttgcc cttggtatag attttataat tgcaaatctc ataattttta attgttcttc
      841 ggtaatgtct ccaagctcat ttaaacaact ttcgacaaac ttgatttttt cttcagaata
      901 catatcgcta atgcttatta aaacaggcgt tatttttagt aaataatccc aggagattgt
      961 ggcgttttgt gttgaactaa caaaattaac ctgaaacatg ttggcattag taaaatttgt
     1021 tgaatccaat ttaaaatgat ttagaatagt attagccata tgtttatctg tgaaatcata
     1081 ataatctaaa tcgacattac gtaagtccag agaggataga tcaactatat ctccagtccc
     1141 acaactaaag attacgttca acctcctgat ttcattgtaa atatctttta atgggcaatt
     1201 aacattgata ttgcctgtag atatttcctg gttttttaac ggttcattaa tggtaagttc
     1261 gcaggtattg tccgatcgtt gaataatttg ggcgttataa cctaaaaaat ccagacgaag
     1321 agacgagtca ttataaagaa ttacattttg taagtacgct aattcttttt gaaaatcacc
     1381 agtgataatg cattttccac ttggttgtaa cgcgttaagt aaagtgacca tttgaataga
     1441 ggtctgtttt agtttttcta taccgggtgc tagtgcttcg aggttatttc ctatggggct
     1501 ttcaggaagg tcattcattt ttcgacggac taaatcacta ctaatatcat taccaattga
     1561 tgaaacaggg aacattattt tatcctttat aaataatcaa taaaaatcat tattaaaatg
     1621 atcgggtgaa aaaaagagaa tttgccaaca atctgaagta atgcaggttt tattaaagtg
     1681 cctagggggt gttaagatgg cttctttttg ggtgaacttt gcgcaatact tagaaatcgt
     1741 ctccttaata cattccagct cttttttatt gacggctgag caagtacatt gttcaccaaa
     1801 aggtgcaaaa gtgcctacat ctaatgtttg tatgatataa tcatcatgtt ctcctgtaga
     1861 aatatctatg atgaatggac tcatattttc cggttggatt cggatcatga cagaaggaac
     1921 atcgccttga tagaaattca atgcagtaat tgcagataat actctccgca gttcattact
     1981 tatactgtaa attttattaa cgtcattggt ggagacaatt tctgcaaact ttctttttac
     2041 cgtccaataa tcaatatttc tattgtgatt gttaaccact gcgtgcattg gcgggattga
     2101 agggtgaaaa atatgtgcaa ttgggaagcc tttcattgat gcctgccttg tcgtcatgac
     2161 aaacaataat agatgcttat tttcctcgca cgacacttac gcactatcaa aaaacgctca
     2221 tttacccggc gttacagtaa acgaagcaac attctgtgtc ctacgctgat gtaaagcaca
     2281 gtgaaaatcc cccttgttga cttcgtctga ccttatcgcg gcgaccagca attattcgtt
     2341 ttgtgatcct caacacaaaa tgctattcgt cgcctatgag gggagaagaa tttatggaaa
     2401 acgattgcat tcacaaaatg acgtcctgtc agaagagaaa aagttgacgt taatcaattg
     2461 ttcgagatga tcaatacctg tattcgaaat tttaaaatat tgataaaata aatgtttgtt
     2521 ctccaacaaa tagcgtgaaa aaatgtgacg tgccgcagta tttatcgctg aaaatggttt
     2581 atttataata aaatttgaga acttgctcat taaatttaac tcaaattttg cctggtaatt
     2641 atccggtaat tgcttgaaat atggtctcag cccctttttt gtattaacca cataacgaat
     2701 gatgttatcg ccataaaata tggttatccc cgtcattttt tttgatagaa atcagggttt
     2761 atgctgattt ttatgcttta acttgttgat atttaaaggt atttaattgt aataacgata
     2821 ccttggaaag taatgaaagt taatttgtga gtggtcgcac atatcctgtt tatttcattt
     2881 tgatacactt catgccgtca atgaggtaat taacgtaggt cgttatgagc actattcttc
     2941 ttccgaaaac gcagcacctg gtagtctttc aggaagtcat tagaagtggc tctatcggct
     3001 cggctgcaaa agaattaggg ttaactcaac cggccgtcag taaaatcatt aacgatattg
     3061 aagattattt tggtgtggaa ttagtggtgc ggaaaaatac cggtgtaaca ttaacacctg
     3121 ccggtcaatt gttactctcc cgttccgaat ccattacccg tgaaatgaaa aatatggtta
     3181 atgagataag cggtatgtct tctgaggcgg tggtggaagt ctcatttggt tttccttcat
     3241 tgattggttt tacttttatg tcagggatga tcaacaagtt caaagaggtg ttcccgaaag
     3301 cgcaggtttc tatgtatgaa gcgcaactgt cttcgttctt accggcaatc cgcgacggtc
     3361 gactggattt tgcgattggt acgttaagtg cagaaatgaa gcttcaggat ttacatgttg
     3421 agccgctgtt cgagtccgag tttgtgctgg tagccagtaa gtcccgaaca tgcaccggca
     3481 ccaccacgct ggagtcgttg aagaacgaac agtgggtgtt gccacaaact aatatggggt
     3541 actacagcga actgcttact acgttacaaa gaaatggcat cagtattgaa aacatcgtta
     3601 aaaccgactc agtcgtgaca atttataatc ttgttctcaa tgctgatttc ttaactgtaa
     3661 ttccttgtga tatgacgtca ccttttggtt ctaatcaatt tattactatt ccggttgaag
     3721 aaacattacc tgtggcacaa tatgccgcgg tatggtcgaa aaattatcgt attaaaaaag
     3781 cagcatcggt tttggtggaa ttagccaaag agtattcatc ttataatggg tgtagacgaa
     3841 ggcaattaat agaagttggt tagttatttg tttttattta aacataaata atccacctgt
     3901 ctgttttgca ggtgtcggtt acggttacct acatatttaa ttcaggcgaa gaggttttat
     3961 aatgcatatt acatacgatc tgccggttgc tattgatgat attattgaag cgaaacaacg
     4021 actggctggg cgaatttata aaacaggcat gcctcgctcc aactatttta gtgaacgttg
     4081 caaaggtgaa atattcctga agtttgaaaa tatgcagcgt acgggttcat ttaaaattcg
     4141 tggcgcattt aataaattaa gttcactgac cgatgcggaa aaacgcaaag gcgtggtggc
     4201 ctgttctgcg ggcaaccatg cgcaaggggt ttcgctctcc tgcgcgatgc tgggtatcga
     4261 cggtaaagtg gtgatgccaa aaggtgcgcc aaaatccaaa gtagcggcaa cgtgcgacta
     4321 ctccgcagaa gtcgttctgc atggtgataa cttcaacgac actatcgcta aagtgagcga
     4381 aattgtcgaa atggaaggtc gtatttttat cccaccttac gatgatccga aagtgattgc
     4441 tggccaggga acgattggtc tggaaattat ggaagatctc tatgatgtcg ataacgtgat
     4501 tgtgccaatt ggtggtggtg gtttaattgc tggtattgcg gtggcaatta aatctattaa
     4561 cccgaccatt cgtgttattg gcgtacagtc agaaaacgtt cacggcatgg cggcttcttt
     4621 ccactccgga gaaataacca cgcaccgaac taccggcacc ctggcggatg gttgtgatgt
     4681 ctcccgcccg ggtaatttaa cttacgaaat cgttcgtgaa ttagtcgatg acatcgtgct
     4741 ggtcagcgaa gacgaaatca gaaacagtat gattgcctta attcagcgca ataaagtcgt
     4801 caccgaaggc gcaggcgctc tggcatgtgc tgcattatta agcggtaaat tagaccaata
     4861 tattcaaaac agaaaaaccg tcagtattat ttccggcggc aatatcgatc tttctcgcgt
     4921 ctctcaaatc accggttttg ttgacgctta attaattcgt tgaggatagg atatgagtac
     4981 ttcagatagc attgtatcca gccagacgaa acaatcgtcc tggcgtaaat cagataccac
     5041 atggacgtta ggcttgtttg gtacggcaat cggcgccggg gtgctgttct tccctatccg
     5101 cgcaggtttt ggcggactga tcccgattct tctgatgttg gtattggcat accccatcgc
     5161 attttattgc caccgggcgc tggcgcgtct gtgtctttct ggctctaacc cttccggcaa
     5221 cattacggaa acggtggaag agcattttgg taaaactggc ggcgtggtta tcacgttcct
     5281 gtacttcttc gcgatttgcc cactgctgtg gatttatggc gttactatta ccaatacctt
     5341 tatgacgttc tgggaaaacc agctcggatt tgcaccgctg aatcgcggct ttgtggcgct
     5401 gttcctgttg ctgctgatgg ctttcgtcat ctggtttggt aaggatctga tggttaaagt
     5461 gatgagctac ctggtatggc cgtttatcgc cagcctggtg ctgatttctc tgtcgctgat
     5521 cccttactgg aactctgcag ttatcgacca ggttgacctc ggttcgctgt cgttaaccgg
     5581 tcatgacggt atcctgatca ctgtctggct ggggatttcc atcatggttt tctcctttaa
     5641 cttctcgcca atcgtctctt ccttcgtggt ttctaaacgt gaagagtatg agaaagactt
     5701 cggtcgcgac ttcaccgaac gtaaatgttc ccaaatcatt tctcgtgcca gcatgctgat
     5761 ggttgcagtg gtgatgttct ttgcctttag ctgcctgttt actctgtctc cggccaacat
     5821 ggcggaagcc aaagtgcaga atattccagt gctttcttat ctggctaacc actttgcgtc
     5881 catgaccggt acgaaaacaa cgttcgcgat tacactggaa tatgcggctt ccatcatcgc
     5941 actcgtggct atcttcaaat ctttcttcgg tcactatctg gggacgctgg aaggcttgaa
     6001 tggtctgatt ctgaagttcg gttataaagg tgacaaaacc aaagtgtcgc tgggtaaact
     6061 gaatactctc agcatgatct tcatcatggg ctccacctgg gttgttgcct acgccaaccc
     6121 gaacatcctc gacctgattg aagccatggg cgcaccgatt atcgcatccc tgctgtgcct
     6181 gttgccgatg tatgctatcc gtaaagcgcc gtctctggcg aaataccgtg gtcgtctgga
     6241 taacgtgttt gttaccgtga ttggtctgct gaccatcctg aacatcgtat acaaactgtt
     6301 ttaatccgta actcaggatg agaaaagaga tgaatgaatt tccggttgtt ttggttatta
     6361 actgtggttc gtcttcgatt aagttttccg tactcgatgc cagcgactgt gaagtattaa
     6421 tgtcaggtat tgccgacggt attaactcgg aaaatgcatt cttatccgta aatgggggag
     6481 agccagcacc gctggctctc cacagctacg aaggtgcatt gaaggcaatt gcatttgaac
     6541 tggaaaaacg gaatttaaat gacagcgtgg ccttaattgg ccaccgcatc gctcacggcg
     6601 gcagtatttt taccgagtcc gccattatta ccgatgaagt cattgataat atccgtcgcg
     6661 tttctccact ggcacccctg cataattacg caaatttaag tggtattgaa tcggcgcagc
     6721 aattatttcc gggcgtaact caggtggccg tatttgatac cagtttccac cagacgatgg
     6781 ctccggaagc ttatttatac ggcctgccgt ggaaatatta tgaagagtta ggtgtacgcc
     6841 gttatggttt ccacggcacg tcgcaccgct atgtttccca gcgcgcacat tcgctgctga
     6901 atctggcgga agatgactcc ggcctggttg tggcgcatct tggcaatggc gcgtcaatct
     6961 gcgcagttcg caacggtcag agtgttgaca cctcaatggg aatgacgccg ctggaaggct
     7021 tgatgatggg tacccgcagt ggcgatgtcg actttggtgc gatgtcctgg atcgccagcc
     7081 aaaccaacca gagcctgggt gacctggaac gcgtagtgaa taaagagtcg ggattattag
     7141 gtatttccgg tctttcttcg gatttacgtg ttctggaaaa agcctggcat gaaggtcacg
     7201 aacgcgcgca actggcaatt aaaacctttg ttcaccgaat tgcccgtcat attgccggac
     7261 atgcagcttc attacgtcgg ctggatggaa ttatattcac cggcggaata ggagagaatt
     7321 caagtttaat tcgtcgtctg gtcatggaac atttggctgt attaggcgta gagattgata
     7381 cagaaatgaa taatcgatct aactcttttg gcgagcgaat tgtttccagt gaaaatgcgc
     7441 atgtcatttg cgtcgttatt ccgactaacg aagaaaaaat gattgctttg gatgccattc
     7501 atttaggtaa agttaacgcg cccgcagaat ttgcataatt tagttgaagt attgtagaga
     7561 gattattttt catgaaggta gatattgata ccagcgataa gctgtacgct gacgcatggc
     7621 ttggctttaa aggtacggac tggaaaagcg aaattaatgt ccgcgatttt attcaacata
     7681 actatacacc gtatgaaggc gatgaatctt ttctcgccga agcgacgcct gccaccacgg
     7741 aattgtggga aaaagtaatg gaaggcatcc gtatcgaaaa cgcaacccac gcgccggttg
     7801 atttcgatac caatattgcc accacaatta ccgctcatga tgcgggatat attaaccagc
     7861 cgctggaaaa aattgttggc ctgcaaacgg atgcgccgtt gaaacgtgcg ctacacccgt
     7921 tcggtggcat taatatgatt aaaagttcat tccacgccta tggccgtgaa atggacagtg
     7981 aatttgaata tctgtttacc gatctgcgta aaacccataa ccagggcgta tttgatgttt
     8041 actcaccgga tatgctgcgc tgccgtaaat ctggcgtgct gaccggttta ccagatggct
     8101 atggtcgtgg gcgcattatc ggtgactatc gccgcgtagc gctgtatggc atcagttatc
     8161 tggtgcgtga acgcgaactg caatttgccg atctccagtc tcgtctggaa aaaggcgagg
     8221 atctggaagc caccatccgt ctgcgtgagg agctggcaga gcatcgtcat gcgctgttgc
     8281 agattcagga aatggcggcg aaatatggct ttgatatctc tcgcccggcg cagaatgcac
     8341 aggaagcggt gcagtggctc tacttcgctt atctggcggc agtgaaatcg caaaatggcg
     8401 gcgcgatgtc gctggggcgc acggcatcgt tcctggatat ctacattgag cgcgacttta
     8461 aagctggcgt cctcaatgag cagcaggcac aggaactgat cgatcacttc atcatgaaga
     8521 tccgtatggt acgcttcctg cgtacaccgg aatttgattc gctgttctcc ggcgacccaa
     8581 tctgggcgac ggaagtgatc ggcgggatgg ggctggacgg tcgtacgctg gtgaccaaaa
     8641 actccttccg ctatttgcac acctgcacac tatggggccg gcaccggaac ctaacctgac
     8701 cattctttgg tcggaagaat taccgattgc cttcaaaaaa tatgccgcgc aggtgtcgat
     8761 cgtcacctct tccttgcagt atgaaaatga cgatctgatg cgtactgact tcaacagcga
     8821 cgattacgcg attgcctgct gcgtcagccc aatggtgatt ggtaagcaaa tgcagttctt
     8881 tggtgcacgc gctaacctgg cgaaaacgct gctctacgca attaacggcg gggtggacga
     8941 gaagctgaag attcaggtcg ggccgaaaac agcaccgctg atggacgacg tgctggatta
     9001 cgacaaagtg atggacagcc tcgatcactt catggactgg ctggcggtgc agtacatcag
     9061 cgcgctgaat atcattcact acatgcacga caagtacagc tacgaagctt cgctgatggc
     9121 gctgcacgat cgtgatgtct atcgcactat ggcatgcggc atcgcgggcc tgtcggtggc
     9181 gacggactcc ctgtctgcca tcaaatatgc ccgcgtgaaa ccaatccgtg acgaaaacgg
     9241 cctggcggtg gactttgaaa tcgacggtga atatccgcag tacggcaaca acgacgagcg
     9301 cgtagacagc attgcctgcg acctggttga acgctttatg aagaaaatta aagcgctgcc
     9361 aacctatcgc aacgccgtcc ctacccagtc gattctgact atcacttcta acgtggtgta
     9421 cggccagaaa accggtaata cgccggacgg tcgtcgcgcc ggaacaccgt tcgcgccggg
     9481 cgctaacccg atgcatggtc gtgaccgcaa aggtgccgtg gcctcattga cgtcggtggc
     9541 gaaactgccg ttcacctacg ccaaagatgg gatctcgtac accttctcaa tcgttcctgc
     9601 ggcgctgggc aaagaagatc cagtacgtaa aaccaacctt gtcggcctgc tggatgggta
     9661 tttccaccac gaagcggatg tcgaaggcgg tcaacacctc aacgtcaacg taatgaatcg
     9721 ggaaatgctg ctggatgcca tcgagcaccc ggaaaaatat cctaacctga caatccgtgt
     9781 ctctggctac gccgtgcgct tcaacgcact gacccgtgaa cagcaacagg atgttatttc
     9841 acgtactttt acccaggcgc tctgacgccg gaggatgtat gaaaaagatt atcgaaacgc
     9901 aacgtgccct aggcgcaatc ggcccttatg ttcagggcgt tgatttaggc agcatggtct
     9961 tcacctccgg gcaaattccg gtttgcccgc agaccggtga gatcccggct gatgtgcaag
    10021 atcaggcgcg tttaagcctc gaaaacgtca aagcgatcgt ggttgctgcc gggctgagcg
    10081 tgggcgatat catcaagatg accgtgttca tcaccgatct gaatgatttt gccaccatca
    10141 atgaagtcta taagcagttc ttcgatgagc atcaggcgac ctacccgacc cggagctgtg
    10201 tgcaggttgc gcgtttgccg aaagatgtga agctggaaat tgaagctatc gcagtacgta
    10261 gtgcgtaaag cctcgtgagc gggacggtcg caaggtcgtt ccgctccact tcactgaacg
    10321 gcaatccgag ggtgtggata tgattagtgc attcgatatt ttcaaaattg ggattggtcc
    10381 ctccagttcg cataccgtgg ggccaatgaa tgccggaaaa agttttattg atcggctgga
    10441 aagtagcggc ttattaaccg cgacgagcca tattgtggtc gatctgtacg ggtcgttgtc
    10501 actgacgggc aaaggccatg ccacggatgt cgccattatc atgggactgg caggaaacag
    10561 tccgcaggat gttgtcattg atgagatccc tgcatttata gagttagtaa cgcgcagcgg
    10621 gcggctgcca gtggcatctg gtgcgcatat tgttgatttt cctgtagcaa agaacattat
    10681 cttccatccc gaaatgttgc ctcgccatga gaacggaatg cggatcactg cctggaaggg
    10741 acaggaagcg ctattaagta aaacctatta ctctgtcggc ggcgggttta ttgtcgaaga
    10801 agaacacttc ggcctgtcgc acgatgtcga aacgtccgta ccttacgatt tccactcagc
    10861 aggtgaactg ctgaaaatgt gtgattacaa cggcctgtct atatctggcc tgatgatgca
    10921 caacgagctg gcgctgcgca gcaaagcgga aattgacgca gggtttgccc gtatctggca
    10981 agtgatgcat gacggtattg aacgcgggat gaacactgaa ggcgtgctgc ctggtccgct
    11041 caatgtgccg cgtcgtgccg tagcgctgcg tcgtcagctg gtttctagcg ataacatctc
    11101 taacgatccg atgaatgtca tcgactggat caacatgtac gcgctggcgg ttagtgaaga
    11161 aaacgcagct ggcgggcgcg tggtaacggc accgactaac ggtgcgtgcg gcattattcc
    11221 ggcagtgctg gcttattacg ataagttccg tcgtccggta aacgagcggt caattgcccg
    11281 ctattttctg gcagcggggg ctattggcgc gctgtataaa atgaacgcct ccatctctgg
    11341 cgcggaggtc ggctgtcagg gggagattgg cgtggcctgt tcaatggcag cggcagggtt
    11401 aactgaacta ctgggcggca gtccggcgca ggtatgcaat gcggcggaaa tcgcgatgga
    11461 gcataacctt gggctgacct gcgatccggt tgccggacag gtacaaatcc cgtgcattga
    11521 acgtaatgcc attaatgccg tgaaagcagt aaacgccgcg cggatggcga tgcgccgcac
    11581 ctcggcaccg cgtgtttcac tcgataaagt gatcgagacg atgtatgaaa ccggcaaaga
    11641 tatgaacgat aaataccgcg aaacatcacg cggaggactg gccattaaag tggtctgcga
    11701 ctgacattgc tgtcagcttt catccttggg tgcacaaatg tgcacctttt ttttgtgatc
    11761 tgccgcaaat agagaatgaa tattctcttg cgattaattt agtagaaaat tcttatccat
    11821 ctccgttatt ttaaagtctt gattgtgaag attttctctg acctcgcgcg tatcatttct
    11881 ttattctaat aactcgcctg caaaaacgta gaccgcatat cgccttgatg cggctcggaa
    11941 aaaaataata agccctacaa aatattgagc gagaaattat ggaaattgca tcgaataaag
    12001 gcgtcattgc agacgcttcg accccggcgg gtcgtgctgg aatgagtgag agcgagtggc
    12061 gagaagcgat caaattcgac agtactgaca ccggctgggt gattatgagt atcgggatgg
    12121 cgattggcgc ggggattgtt tttctcccgg tgcaggtcgg tttgatggga ttgtgggtat
    12181 ttttgctctc atcggtgatt ggttacccgg caatgtatct gtttcagcgg ttgtttatta
    12241 atacgctggc agaatcacca gaatgtaaag attacccgag tgtcattagc ggttatttag
    12301 gtaaaaactg gggcatcctg ttaggtgcgc tctatttcgt aatgctggtg atttggatgt
    12361 tcgtctattc caccgccatc accaacgata gtgcttccta tctgcatacc ttcggcgtga
    12421 cggaagggtt gctgtcagac agtccctttt atggtctggt actgatttgc attctggtgg
    12481 cgatctcttc acgcggcgag aaattgttat tcaaaatttc gaccgacatg gtgctgacca
    12541 agctgctggt agtcgctgcg ctgggcgtgt cgatggtggg aatgtggcat ctgtataacg
    12601 tcggttcgct accgccgctg gggctgctgg tgaaaaacgc cattattacg ctgccgttta
    12661 ccctgacgtc gattctgttt atccagacgt taagcccgat ggtgatctct tatcgctcgc
    12721 gggaaaaatc aattgaagtg gcgcggcata aagcattgcg ggcaatgaat atcgcgtttg
    12781 gcattttgtt tgtcaccgtc tttttctact ccgtgtcgtt cacgctggcg attgccgcac
    12841 agtttattag cggtgacggc gcagcgtggg tgaaagtggt cagcgtcatt ctcaatatct
    12901 ttgctgtcat gactgcattc tttggcgtct atttaggctt tcgcgaagca acgcaaggga
    12961 tcgtaatgaa catcctgcgt cgcaagatgc ctgccgagaa gattaacgaa aatcttgttc
    13021 agcgcggcat catgattttc gccattttgc tggcctggag cgccatcgta ctgaacgcac
    13081 cggtgttgag cttcacctct atctgtagcc cgattttcgg catggtaggg tgcctgatcc
    13141 cggcgtggct ggtttacaaa gtaccggcat tgcacaaata caaagggatg tctctgtacc
    13201 tgattatcgt cactggtttg ttgctttgtg tttctctgtt cctggcattt tcttgattta
    13261 cctgaaattt taaggttttt aatatgtttg attcgacttt aaatccgtta tggcagcgtt
    13321 acatcctcgc cgttcaggag gaagtaaaac cggcgctggg atgtactgaa ccgatttcac
    13381 tggcgctggc ggcggcggtt gctgcggcag aactggaagg tccggttgaa cgtgtagaag
    13441 cctgggtttc gccaaatctg atgaagaacg gtctgggcat caccgttccc ggcacgggaa
    13501 tggtggggct gccgattgcg gcggcgctgg gggcgttagg tggaaatgcc aacgccgggc
    13561 tggaagtgct gaaagacgca actgcgcagg caattgccga tgccaaagca ctgctggcgg
    13621 cggggaaagt ctctgttaag atccaggaac cttgcaatga aatcctcttc tcacgcgcca
    13681 aagtctggaa cggtgagaag tgggcgtgtg tcaccatcgt cggcgggcat accaacattg
    13741 tgcatattga gacgcacaat ggtgtggtgt ttacccagca ggcgtgtgtg gcagagggcg
    13801 agcaagagtc gccgctgacg gtgctttcca gaacgacgct ggctgagatc ctgaagttcg
    13861 tcaatgaagt cccatttgcg gcgatccgct ttattctcga ttccgcgaag ctaaattgtg
    13921 cgttatcgca ggaaggtttg agcggtaagt gggggctgca tattggcgcg acgctggaaa
    13981 aacagtgcga gcgcggtttg ctggcgaaag atctctcttc atccattgtg attcgtacca
    14041 gcgcggcatc cgatgcgcgt atgggcggcg ctacgcttcc ggctatgagt aactccggct
    14101 cgggtaacca ggggatcacc gcaacaatgc ctgtggtggt tgtagcagaa cacttcggag
    14161 cggatgatga acgactggcg cgtgcgctga tgctttctca tttgagcgca atttacatcc
    14221 ataaccagtt accgcgtttg tctgcacttt gtgccgcaac gaccgcagca atgggggccg
    14281 ccgccgggat ggcatggctg gtggatgggc gttatgaaac tatctcgatg gcgatcagca
    14341 gtatgatcgg cgatgtcagc ggcatgattt gcgatggtgc gaaggtttcg accagtgctt
    14401 cggctgcgtg gaaagcggtg ttaatggcgc tggatgatac cgccgtgacc ggcaatgaag
    14461 ggatcgtggc gcatgatgtt gagcagtcga ttgccaacct gtgtgcgtta gcaagccatt
    14521 cgatgcagca aacggatcgg cagattatcg agattatggc gagcaaggcc agataaacgc
    14581 caggttttga gtattgtgcc ggatgcgatg ctggcgcacc ttatccggcc tacggggtgc
    14641 tcaacttgtt gatgttgttg tgttttgtac ctgatgccgg atgcgatgct ggcgcatctt
    14701 atccggccta cggggtgttt aatttgttga tgttgttgtg ttttgtaggc cggataaggc
    14761 gtttacgccg catccggcaa cctgtcttta cgcggtagct tcatcctcgg ctaaacgcgt
    14821 ttcagcatcc gcgacgatag cttccagctc gctcaacatc tcttcatagc caaaattttt
    14881 ggcggtgcga gcaggttctt tcgccaccac gggcttcgct agctggcgct ttttggtcga
    14941 ttttttactc atcatcatct ccctgtaaaa acaactagac aggcaaatct atcagcaaag
    15001 cgcgcagtgg ggaatcggca accagcgtaa tgttagcctc gtcacgaata aacgccccat
    15061 caccgcaggt tagcgccgct ttctcttcat gatgcgtaag cgcatgaaat ttcccgtgaa
    15121 tcgattgcaa atacgcgcgt ggcccatgca actggaaatt cgcactttcg cctttgtcga
    15181 gcacgatatg gtgcagccac acttgttggc gtaattgcag gcttcccatc gcaccctctg
    15241 gcgaggcgat taattgctgc ttgtccatat taagcgccag cttttgaatc agcggattct
    15301 ctcgctgcgg gcaggtatcc agccaaagct gcattcgcgt taacggtttg tctttgctga
    15361 gattgtgttc gctatagctg acacccggct gggtagagag cagcaacgcc tctccggcgc
    15421 tggcctgaac atgattgcct tcgctgtcgc gatactctgc ttctccatcc agaatcacat
    15481 ttaaaatatc gactttggga taggttcgcg gctgaaaggc ggcacctggg gccagcactt
    15541 cctggttaag cacacgcagg gaggcatagc ctaacaattt cgggtcgaag tagtgtccaa
    15601 aggaaaaagt ataccgggcc tgcaaccatc cgtagtctgc ttgtccacac tgcctggcag
    15661 ttcgggtagt aatcataatt cttgacctct ctttacttcc ttttatggta aggggctgga
    15721 cgctacattg ttagccagat attctgcccg gtatgttcaa atttcctgaa tgagaacgaa
    15781 atggccaaag aaagggcatt aacgctggaa gcactacggg ttatggatgc gatcgatcgc
    15841 cggggcagtt ttgcggcggc agcggatgag ctgggacgcg tgccttccgc acttagctac
    15901 atcatgcaaa aactggaaga agagctggat gtggtgctgt ttgaccgctc gggccatcgc
    15961 accaaattca ccaatgtcgg gaggatgttg ctggagcgag ggcgcgtttt gctggaagcc
    16021 gcagataaac tgactactga tgcggaagct ctcgcgcgcg gttgggaaac gcatctcacc
    16081 attgtgaccg aagcgctggt accgacacct gcctttttcc cgttaatcga caaactggcg
    16141 gcaaaagcca atacccaact ggcaatcatc acagaagtgc tggcgggggc gtgggaacgg
    16201 ctggagcagg ggcgggcgga tatcgttatc gcgccggata tgcattttcg ttcctcgtcg
    16261 gagatcaact cgcgcaagct ctatacgtta atgaacgtct acgttgccgc gcctgatcac
    16321 ccgattcatc aggagccgga accgttatct gaagtgacgc gtgtgaaata tcgtggaatt
    16381 gcggtagcgg ataccgctcg tgagcgcccg gtgttgaccg tacagctgct ggacaaacag
    16441 ccgcgcttaa cggtgagcac gattgaagat aaacgtcagg cattactggc ggggcttggc
    16501 gtggcgacga tgccgtatcc gatggtcgaa aaagatattg cggaagggca gttgcgtgtc
    16561 gtcagcccgg aatcgaccag cgagatcgat attattatgg cctggcgtcg tgacagtatg
    16621 ggggaagcga aatcctggtg tctgcgggaa attccaaagt tgttcgctgg aaaataatct
    16681 attaagcacc tttcacaagg tgcttctgac tgctgacaaa cgcaaaactg cctgatgcgc
    16741 tacgcttatc aggcctacgc agtcactgca atatattgaa ttttggcaat ttgtaggctg
    16801 gataaggcgt ttacgccgca tccggcataa acaaagcgca ctttgtcaac aatttgaagc
    16861 accttttaca aggtgcttaa tcattattct tgttccagtt ttggatctgg tccaaagcgg
    16921 ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca gccgataaac
    16981 gggattaaga atagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag gcggcggaac
    17041 tgaaccgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag gatgccttcg
    17101 ccaccagcgc gttgccagcc taacatttta tccagcagcc ccagcacgaa cgtaaagatg
    17161 atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg gaaaccgaca
    17221 taatttttta gtactttcag ataccagtcc atttttgcct caattttgct caatcacttg
    17281 tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata ttcgttcaag
    17341 gaattaataa agtgccgtga aggcgcgcta agactgatgg caagcgcaaa atcgccagat
    17401 gcgctacgct tatctggcct acgcggtcat tgtaacatat tgaatataaa cgctttgtag
    17461 gtttgataag gcgttcacgc cgcatccgac atgaacaaca tgcacttagc taattatcag
    17521 aggcacctcg taaggtgcct ttttttaacc gaagcgaaca tctcgtccat gcggttcatc
    17581 gagatcctgc cacggaccaa ttgaaataat ccccgtaggg ttgatggtct tatggctgcg
    17641 gaagtaatga ttacggatgt gatcgaaatt gacagtttcg gcgatccccg gcatctggta
    17701 gatatcgcgc aggaagccat acagattcag gtaatcgctg atgcggtgct tatcacactt
    17761 gaagtgggtc acatacactg gatcaaaacg caccagcgta gtccacaggc gaatatcggc
    17821 ttcggttagc tggttgccgg tcaggtaacg atgctggcct aaaatctgct ccagtcgcgc
    17881 cagcgattca aacactttcg ccaccgcctc gtcgtaggct tgctggctgg tggcaaaacc
    17941 agctttatac acgccgttgt taacggtgtc ataaatccag ccgttaagtt cgtcaatttt
    18001 cgtttgcagg gctggtgggt agtaatcacc cgcttttgcg cccagcgcat caaacgcggt
    18061 attgaacatg cggatgattt ctgctgattc gttgctgacg atggtgtggt tctttttgtc
    18121 ccacagcacg ggaacagtaa ctcgtccgct gtagtgtgga tcggcgtgga gataaagctg
    18181 atacagaaat tcatgttgat agagcgtatc tccggttgct cccggaaaac tgtcatcaaa
    18241 ggtccagccg ttttccagca tcagcgggtt cactacggaa acggaaataa acggttccag
    18301 tcctttgagt ttgcgcatga tcagcgtgcg gtgcgcccac gggcaggcga gtgaaacata
    18361 gagatgataa cgatctttct ctgcgataaa accgcctgtg ccagtggggc caggtgcgcc
    18421 atcggcagtg agccagttac gaaatgcgga agctgaacgt tgaaatttac cgccggtaga
    18481 tttggtatcg taccaggtgt catgccagac gccgtcaatc agttgaccca tttttctctc
    18541 ctccaatagc aaaaacgagg agcttcctcc tcgtttttta tcgttaagta tagcgcgtgc
    18601 ttaccacttt ttattcagca ggcggtcgat gctatacgcg cccggaccgg taattgccag
    18661 cagcaggaat ccgccagaaa ttgtcaggtt tttcatgaac atcagcgagt tgacgccttc
    18721 agcaaagttg ctgtgaaata aaaatgccgt cagcagcgta aagcccgcag taaacagggt
    18781 tgtggtgcga gtcaggaaac tgaataggat tgccagacca ccaccgaact caagcagaat
    18841 caccagcggc agcataaaac ccgggacacc cattgcttcc atatattgtt gggtaccaga
    18901 gtaagcggtg atttttcccc agcctgcggt aataaacaga atcggcatta aaatgtgcgc
    18961 taccagtaca ccaacatctt ctaatttttt catcatactc tccactgcgc cactcaggct
    19021 gctgattgtt tttttgtgca agggcgcggt attagcgtcg ttgctgtcga tggagagaat
    19081 cataaacgtg gtgaatgatg attgttagca aggaaaactg tcaaaaatat tcaaaaaatt
    19141 tgagggataa ggccggaatt gctccggcca gagggaagtt aaccgcgaag ctgttgctgc
    19201 ttgagggtcg ttttaaccag acgccaggcg ctccatacgc caaaaccgcg tctggcccag
    19261 cggaccagca tattaggatg gcgaatcgtc cagatcgcca tcacgctact gccaaccagc
    19321 gcccaggagc gcagacttag cagcatattc cagcgacgat cgtaagcgcc tgttgcctcc
    19381 agccattcac gacgactggc ggaaagatcc agccgttgtt gctggatctg gctaagcagt
    19441 tgcgccttac gtcgttcacg ttcgacttta ctgctcacgg gactcctcct cgagcagttg
    19501 ccgatcgttt gccagctcat ggcgtgtatg gcgcagcaag gtggatttac gcgatttacg
    19561 taacgtccag ataccgccaa tcagtgccag tagcagcaac accacggtgg tggcaatcat
    19621 cgcattcagg cgatattgcg gatcaaccgc ccaaataatt agcaccatca ggctcataag
    19681 accaaatgca gcgaaaagca tcgtcaggcc cagcatcagt aaaagttgaa agagattagc
    19741 tttttcctct tccagctcca ccaccgccag ccgcagacgt gtttccacca tttcaaccat
    19801 gatggaaaca attcgctgcc cgatgcccag aacgctttta ccgggccctt gcgcgtgatg
    19861 agtgtccgcc ataattaacg acgcgacagc agaacgccga gcactacacc gattgcagcg
    19921 ccaattccca cgccggtcca cggattttca cgcacatact catcagcacg cgccgccgcg
    19981 acacgggttt gtttggcgat ggcatcaccg gtttcaccca ggcgataacg gctctgtttc
    20041 agtgcctgct ccgctttgct acgaatctta ctcaactctt ctttcgactt ctcgccagat
    20101 gagctaagca cctcttccag cgtatcggaa agggatttca actcagcacg cagatgttcc
    20161 gtagtgtgtt ctttcgacat agttttctcc aggtgagtaa gtagtgacta ttgtgagtta
    20221 gtagtcgcgc gcttccagct ttttcagctc ttcctgcgct tctgccagtt tccgttctat
    20281 cggcatcgcc tttttgcttc gcctcggcta aatcttgctg gcgttccgcc acctcatctt
    20341 tctgctttgc gattttcttg tgatgatcgg cacgcagctg gctatctgaa cagttggccc
    20401 ggacttcact cagggcttta ttcagaccgt caatacgatt ctggttttgg tgtttttcgg
    20461 catagctgat ctccttaagg atattttgct ccttttcctg acacagggta gtggcataac
    20521 taccggcact aagagcaaag agagaaacag ctaaagcgat gcggtatttc attcttgaac
    20581 cttccattgt gattcggacc tattacaaag gaacgattgt ccagacaacg aagcggtagt
    20641 gttaaccacc gcctcattaa tcatagacag ttacggtgaa atcaccaaag tgagtgatat
    20701 gtttcggata acaggaagtt atccgaagcg atgagagtta tcccgtaacc gggtcagcca
    20761 ctgcatagcc tgactgttat tgtcctgctg cgcaatgatg tagccatggg gcaatgttct
    20821 gtcgaggacc gcttttgcgg cggcgctctg gtcgctggag tcaaatgtaa ttaacagagt
    20881 gtcgtttttg ggggtgatac ttttgaaagg aatgccatga gcgtcaaggt gatgccagat
    20941 cgaaaaaccg tctggcatcg ttgtgccttg atgaacggca cgaatcgcca gcgtagactc
    21001 ttgctggcga accgcggacc aggccagcaa cagcgtgccg accagaagta aaacagtgcc
    21061 ggaccaggct agctggcgaa gcgacatgcg aggtatttgc atcccttacc cccgatttcc
    21121 atatttcttt ttccataaca cgaccagaga acctgccagg ccaaacacca gcagcaccac
    21181 cgggagcagc atcaggcatg acatcagctg gtcctcgtac tttaaaaata ccggcgtttt
    21241 gccgagcatg taacccagag ttgtcaggat caatacccac agcagaccgc tcatccagtt
    21301 gaagaactga aagcgcgcgt tattcagccc tgataacccg gcaatcgtcg gcagcagtgt
    21361 tctgacaaat gcaataaagc gaccaattaa cagcgccgat aaaccgtgtt tatgaaaaag
    21421 atggtgtgcg cgttgatgat aatgcgcggg taaatgagat agccagtttt gtacggtgcg
    21481 ggtattgccc agccatcgcc cctgaatata gctgacccag caaccgaggc tggcggcaac
    21541 ggtcagcagc agaatcgttt gcgggtaacc catcgcgcct ttcgcaatca acacgccgac
    21601 caataccagt aaactgtcgc ccggtaaaaa ggccgccgga agcaagccat tttcaaggaa
    21661 caaaattaca aacaagacaa aatacaacat gccaatcatc gatggattgg ccagggtttc
    21721 aaaatcctgc gcccacaggg cttgcagcaa ttgggtcaaa agttccattc attattcctg
    21781 gtgaatcggt taacgccacg ctggttttaa aagatgcact accgcaatgt tattgctgtt
    21841 ttggtatgtt ctgcgggttt gatacatcaa cacagcctga actattccct gttaaaacgt
    21901 gagataagaa agcattcaaa agcataaatt caaattgaat ctaattgtaa caaaggtcaa
    21961 cgttgttcgt tatcaaaatt acatgtccgt ggagtctgat tttgctgatt gcaggggagg
    22021 gtggcgagag gatttacaca ggctgacact ttatactttt cgcttaccga cgagacaaac
    22081 aggcgcattg cagcgcctgt cggacaggaa tttcatttgc tgccgctggt ggccgtatcg
    22141 agatgcacta ccgggttttc ggcgaacaga tagcggtcgg cattgaactc gaaatcgtcg
    22201 ctggtttcgt taaataacat gattttggtg ttttccagat gctgccacat tgccagctta
    22261 gcagcatgag gatctttgcg aattaacgcc ttgagaattt gatcgtggtc atcacaccag
    22321 ttatcaacgg tacgggaatc aatgtgttcg tgtagttttt tccagtacgg gttatggcta
    22381 cgctgggtcc acattttttc aacgatggcc gccagggcgg agttctgcgt cgccagggct
    22441 acctgaatgt ggaactgtaa atcccactcg gaatcacgga agcattgttc gccgcgcgcc
    22501 tgttcctgaa tggccatcag tttcatgatg tcttgtttcg ttacctgagt tgccgcgaac
    22561 tcggcaatat tactttcgat gagctggcga gcctgaagca actcaaacgg accgtaattg
    22621 gcgaattcca tattattgtc agccgcctgc tgatgacgcg gctggttgga aaccacatga
    22681 atacctgaac ctttacgcac ctccacatag ccttcaactt ccagcatgat gatggcttca
    22741 cgaactaccg ttcggctgac gttcttttca tcggcaataa agcgttcggc aggcagttta
    22801 tcacccacca gatagacgcc ctgttcaatg cgctctttca ggtcagcagc aagttgttga
    22861 tacaaacggc gtggttcagt gatttccata tgcgtctccc ggagtggaat gcggacgaag
    22921 ttatcatacc actttattca tgggtatgcc attggtcata tataaatttg atgattgaat
    22981 taatgtatta aattaatatt gtgaatattt ataaattcct aaaaaataag tatcgtcatt
    23041 gtgaaagcta ctaaatttca gtaagttaat tcccagcctt taaatttaat gttaactaaa
    23101 aatgcttttt tatattgact tacaataaat ctacatctct aatacaccaa cttttttgaa
    23161 acgatattgt aagatcaaaa ttatatggtg gcgaaaatta atctcttatt acggcttacg
    23221 ccgcttttat tcgtgttcgc tccttttttg gcacagtcaa atatgactgt ttatcctatg
    23281 cctgtttcca ttaatagtca gggtgaaggc aacgttcgcg taatatctaa aagtaatgaa
    23341 gttcagtaca tcaaggcgac agtattccgt atcgataatc catcgacgcc tcaggaaaat
    23401 gaagttgaga ttaaatccgg tgatgcaaat catctggttg ttatgccacc taaattcgct
    23461 ttaccagccg gtagtagcaa aaccgtacgt tttgttgcga tggaaccaga gcaaaaagag
    23521 aaaaattatc gcgttaaatt tgaagcggtt cccagtattg atgacgttgc cacagataaa
    23581 aaagatctct ctatgcagtt aacagttaac ttaatttggg gaatcgttgt tagtgttcca
    23641 cctcagcaac ctatagctaa gttagaagta aattctgccc aaaaattaat taatgcaggg
    23701 aatcaacgct taaagctttt aacgatcgct tattgtaaaa ataatagcaa agaaaactgt
    23761 aagatacaga ccgtaaataa aaacatcttc cctggtcagg aaagaaatct tgaaagcata
    23821 tctggctacg acaaaatcgt tgtcaaatat aataactgga tcaccaaaga taatggcgag
    23881 tttgaactgg cggtccatta attatcattg taataaacga aggaatagta atgaaaaagg
    23941 tatttgcaaa atctcttctg gtcgcagcga tgttttctgt tgcaggctcc gcattggcag
    24001 tacaaaagga tattactgta acggccaacg ttgatgccgc tctggatatg acacagaccg
    24061 ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct gccggggcag ggtctgcaat
    24121 cttaccagtt gatgaccaaa atctggtcta acgacgttac caaagatgta aaaatgcagt
    24181 tagtgtcccc agcgcaactg gttcaaagcc tggatgctag caagattgtt ccgctgacag
    24241 taacctgggg tggtgaagaa attaaagctg atgcggcaac cacttttacc gcaaccaaaa
    24301 tctttgcttc cgatgcgtta actaacggtt ctctggctaa aaacctgatg tttgctcaaa
    24361 ccaccaaagg tgttctggaa acaggcatct accgtggtgt agtaagcatt tatctgtctc
    24421 aggatattta atccggataa cagaattaac acgtaacaaa gggaggggta gccctccctt
    24481 attttaactg ttttagtatt tgttatggat ttcaggattg ccatggataa aaaattactg
    24541 gctcttttga tcctggcgag tctcagcccg gcagaggcga cattaaccaa aattcccgca
    24601 gggtttgagg ttattgctca gggacagcag gagtatatcg aggtttattt ttcagggaaa
    24661 agtctcggta aatattatgc aatggttaat cttgataccg taacatttct tgatccagca
    24721 agtctatata acaagctgga actggatgta gacgatcaga aaatcgcgca tatagtgaaa
    24781 gaaaaattat cgcagccgct agctcgccac ggtgaattgg cttgcggtta tgtacgtact
    24841 gactcagggt gtggttttct gaataccgat acgctggaaa taatctataa tgatgaagaa
    24901 agttcggcaa cgttgtttat taatccgcaa tggaattcag ctttcgatgc gaagtcatta
    24961 tatttaaatc cagacaaaaa tactgttaac gcttttatac atcagcaaga catcaatgtt
    25021 ctggcacagg atgattacca atcgttgtct attcagggaa acggtgcgct gggaataaca
    25081 gaaaatagct atattggtgc acactggaat ttcaacggtt atgatgcaga tgatgtcagt
    25141 gacagtaata ctgacgtcag cgatctctat tatcgttatg attttttacg tcgttattat
    25201 gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg cacaaggcgg gaactttacc
    25261 tttaactttc tgccactcgg tgcaatcgat gggatgcgta tcgggtcgac actgagctat
    25321 ttaaaccagg cgcaaagcca gcaaggaacc cctgtaatgg ttctgctttc gcgcaattct
    25381 cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt tttatctcaa tagtggttcg
    25441 caatttattg ataccagttc ctttccgcca ggcagctaca gcgtggcatt aaaagtctac
    25501 gaaaataacc aactcacccg caccgagctt gtgccgttta ccaaaaccgg cggtctgact
    25561 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt ttctgatgat
    25621 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
    25681 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
    25741 gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc
    25801 ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac gttgggcttt
    25861 tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag ctataacgcc
    25921 ttaagctgtt atgaaagtat ttccgcgaca gtttcactga attttgtcgg ctggaatatg
    25981 atgctgggtt atacctgcac acaaaataac actgatgata gtttgcgttg ggataaacag
    26041 cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc tgaaactgta
    26101 caacttagcg cttcccgcgc tattgtgatg cgtgactgga ttttgagtac gtcagtcggt
    26161 gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta cttatcgttt
    26221 tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac caatgtgtct
    26281 acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt atcgcatacc
    26341 ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc
    26401 gagacgattt gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa caggatgaag
    26461 agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaactttg cagcttggtc
    26521 aaagtgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt gaggttaacg
    26581 acagtagcca gggcaatcag caggggacaa caaacatcat taacggtgca gggaatcgtg
    26641 aattaatgtt gttgcctggc aagctgcgct atcgcgaagt gtctgccagc tttaactaca
    26701 actatatcgg tcgtttatta ctgccggcag cggtgaaaaa attcccgatt gtggggctga
    26761 acagcgccat gttactggta gctgaagatg gcggatttac acttgaaatt aacggcagcg
    26821 aaaaagagct ctatctgctt tccgggcagc aattccttaa gtgtccactg agtgttgtaa
    26881 agaaacgcgc cagcattcgt tacagcggag atgttacttg tagtgtggtg acttattcac
    26941 aattaccgga gtcgattcag gttcaggcac agttgaaaca gcctaaatta cgtggaaacg
    27001 ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcgg cgatattggg
    27061 cttgtttggc acgctcactg gcgttcaggc agctcctgac gtgaccagtg aaattacgta
    27121 tgatttggca tctggtagag cggattatta cttctggaag gatgaagcgt cggcaggaaa
    27181 taatggatat atgtggtatg aatgttcgta tcctgacctc caacaaacct gtacagctaa
    27241 tggaaatata tcgacagtac aaatctattt aactgaacaa cgcagtggga tgcgttggcc
    27301 ggtaaaactc aaaggattta aaacagccat tgtaagtagc gatgaagcgc cgctaggatg
    27361 caaggggggc aaagggcttc agacgaatct taaggattct aatagatctt catgtacaga
    27421 agatggtcaa cattattata tatacgatac aaagtttctt acgctctacc ttgagcagac
    27481 agagatgaag aatttgccga ttggtggcgt ctggaagggg aaagttaaat tacattcgaa
    27541 cagcccggcc caggactatt tcgcaaatat taccctgaat acgctcgacc ccaaccatat
    27601 tgacgtgttc ttcccggagt tcgcccacgc cacgccaagg gtgcagttag acttgcatcc
    27661 aacaggaagc gttaacggca gcaactacgc gcaagatctg accatgttgg acatgtgcct
    27721 gtacgatggt tttaacggta atgccatcag ttatgaaatc atgctcaaag atgaagggcg
    27781 accagctgca gggcgcagag acggttactt ctctatctat cgtcagggag ggaccaccac
    27841 cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa ccggtgggca
    27901 aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga aacgtgtgcg
    27961 tccggtggta ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac ccttgactct
    28021 ggcagtcgat aaattcagcg tgatggataa acaggccggg tactacatgg gcaaattgtc
    28081 agtaatcttt acgccttcct tgccaaccat caattaaccc tgcaaaccgg acaaacgctt
    28141 ttttctatat aaacgacgaa gccgcctgat aaggcggctt cgtcgttata acttacgtta
    28201 aacgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca atcgccggtt
    28261 tgttctgcaa tacggtccag ataaccagcg cacccagcag gtcaaatact gccagaactg
    28321 cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc gcaaacagcg
    28381 tacttgccag ccatgcggac atcccggtta aaccgttcgc cgtcgccact tcgttacgac
    28441 cgaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtgg gcaaaaccac
    28501 cgatacacag cagcataatt gcgacatacg ggttggtgaa cagaccgatc atacccgggc
    28561 caatcatcag cacagcaccc agcgttacga ccatcttacg ggaaacgatc aggttcacac
    28621 caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg aggtcagcaa
    28681 acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg taaactttaa
    28741 acatgaacag cgggatccac gcgttaaaag taccccaggc cggttctgct aggaaacgcg
    28801 gcagtgcgat accccagaac tgacggttac gcaggatctg accaacggac attttcttcg
    28861 ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt tcttcatcgg
    28921 tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc gcccagataa
    28981 agctcaatgc accggagatg ataaatgcca tctgccagct gtgcatcacg attgcccata
    29041 ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag tagcctactg
    29101 cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc gccgggatca
    29161 tcgctgcttc cgcggcaccg accgcaccac gagcaacagc caggccaccc cagctacctg
    29221 ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca taaccgattt
    29281 tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta taagcagcag
    29341 aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct tccatcagag
    29401 ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg ccgagcgtca
    29461 ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac ctcatcgttt
    29521 tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg ttagttacga
    29581 ccgggtaacc gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt tcgtgctaat
    29641 tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg caatttgcat
    29701 tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc ttaaaaggtg
    29761 agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag cgaggccagt
    29821 actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa ctacgtttat
    29881 tgatctaact cacgaaaata tcttcggact ctggaaattg gtgtgataac tttgtcagca
    29941 tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac tccgtttatg
    30001 actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga ctacgcaaaa
    30061 gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc ggaagactat
    30121 cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg gcgcgctatg
    30181 cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg tgaaaaattt
    30241 gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca ctggacgcac
    30301 ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc aactgccgat
    30361 gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctcctc gcgcggcatc
    30421 atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga ttctctggag
    30481 catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc gagctggcgt
    30541 ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc gaagctgggc
    30601 gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt gaccaaacgt
    30661 ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga cgtggtgatg
    30721 tttgctgaag cgaacgaagc ggaactcgac agcattctgg cgcgccgtct ggctggcgaa
    30781 accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt ccttggtgcc
    30841 gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg taataacaac
    30901 ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa cgaccgtccg
    30961 atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct gctgccgaaa
    31021 accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat gatcggtaac
    31081 ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg gtttaacgat
    31141 cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct gctgagccgt
    31201 tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca cgaatacttc
    31261 cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc accggcggac
    31321 atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg tgactacttc
    31381 gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc catgcgctgg
    31441 ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt gtcgataacc
    31501 agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta acggatatcg
    31561 caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg gcggatattg
    31621 cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat ctggatcagt
    31681 atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct gaagtgcaga
    31741 tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc ctgccaaccg
    31801 tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa gagaccaaca
    31861 acgccgaagg taccgacggc gtgttcctct tcagccacac ctacggctgc tcacaactgg
    31921 gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac ccgaacgcgg
    31981 gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca ttccgtgaaa
    32041 cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag caggatgatg
    32101 agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc aacgataaac
    32161 gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt tctgacggtc
    32221 tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg attgctaacg
    32281 gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag ttgctgatgg
    32341 accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac gacttcaaac
    32401 agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg aacaaagcgg
    32461 gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg ggttccagtg
    32521 tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg aacttgttaa
    32581 gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc tgccatatgg
    32641 tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg gtgaaaatcg
    32701 ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac gcgggtcagc
    32761 tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc atcgttgagt
    32821 ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg gcgatcttca
    32881 aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc gtttcataat
    32941 gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc gtattggctg
    33001 gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt caatcgtgac
    33061 gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt acattttttt
    33121 cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg tacattgatt
    33181 accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac tggcggaatt
    33241 ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac gattgtcagt
    33301 acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg gttttcaacg
    33361 tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat gattgagggt
    33421 agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca gaaaagggga
    33481 attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg ttaaacaatt
    33541 aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct gccgcagtga
    33601 acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat acgccgataa
    33661 taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac atattacagg
    33721 ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag gcacacagag
    33781 aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc agcgtattaa
    33841 ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg atggtggctc
    33901 ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca cacagcgcca
    33961 tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca ccgctttcgc
    34021 gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac accagcaatg
    34081 ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc agttgcgcgt
    34141 agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc ccaaaaatac
    34201 cgatcggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc gacatatcat
    34261 tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg ccgatcgccc
    34321 acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg gaaaccatgc
    34381 tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata tcacccgcgc
    34441 tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg gccagagctg
    34501 cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt ttctgcccgt
    34561 gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg gcaacagctt
    34621 tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg ggttttgaga
    34681 tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc aggctgccat
    34741 gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga tcctttcatt
    34801 gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt
    34861 ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc tggattttaa
    34921 agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca gagggaagga
    34981 ctggctatta aggctgatta gataacatat gggcaaaact atttggaata tgagaacggt
    35041 tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg cgcagggggc
    35101 ggccaatcgc cgccgccccc tgcaccctca ggctctggcg aacaaaatcg ccgctgcgcg
    35161 gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt ccctgtaaga
    35221 tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac caacacccgt
    35281 gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac ctttgtctgg
    35341 aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg ctgcttatca
    35401 tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc aaacaccacg
    35461 cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt gataccgata
    35521 aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc tacgcccgcc
    35581 agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt cagcacaatg
    35641 aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa aatcacgtcg
    35701 ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa tccattctta
    35761 cgcacaaaga aatgctcgtt gtcgatggtg tctgtcatgc gcaaatgacc gcgtagccag
    35821 cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc catcttcacg
    35881 ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg cgaaatcagc
    35941 cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac gccatacacc
    36001 agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat cagccagaca
    36061 aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa ggccagggcc
    36121 tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa agcggcgtta
    36181 aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt catggtcatg
    36241 gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat ggcgacaaca
    36301 acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt tccttacaca
    36361 taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata gacctcgcct
    36421 tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct ttcgggttgc
    36481 ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca atgaagttac
    36541 tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt ctgttacata
    36601 ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg aaaaatcacc
    36661 ccagtctggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac cgccagccct
    36721 ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc gtagagcatg
    36781 gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg cacaaagcag
    36841 actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc ctgaatctcg
    36901 cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa atcaccgtaa
    36961 tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc cgttgcctgc
    37021 acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa gccgatatcc
    37081 ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc gtccaggtaa
    37141 cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag tttgcctgct
    37201 ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt aaatgcctca
    37261 aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc cgccagattc
    37321 gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag ttgtgtctgg
    37381 gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc gctttccgcc
    37441 atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa gtgctgagcc
    37501 tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc atgggcggcc
    37561 tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat cataacgcgt
    37621 ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc ttcgacgtgc
    37681 gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc aatcatgacc
    37741 gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac cgcgctgaca
    37801 cgagcgagtt tctcccgcag ttgagtataa aaaccatgct tcatattgag gtctggcgcg
    37861 ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg ttggttatga
    37921 aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc acgcactacc
    37981 gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg tacgccttta
    38041 gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc gttatcgcca
    38101 ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt atcaatacgt
    38161 cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt acgcgccggg
    38221 acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac gcgatcggcg
    38281 ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag agtccggcga
    38341 gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt tatccttaac
    38401 gaaagggggg aggtcgattt taggcgctaa agaaagaaaa gataacgcct gacatcattt
    38461 tcagattttg tcctgcccaa gcgacaactg cgttagccac agccgggtgt cgaactcaag
    38521 ctggtggtac tgcggctcca tatggcagca caattgatag aacgctttgt tatgctcttt
    38581 ctccttcagg tgcgccagct cgtgcaccac gatcatgcgc aaaaacggtt ccggcgcgtt
    38641 gcgaaacacg gtagcgacgc ggatctctgc ttttgctttc agcttgccgc cctgcatacg
    38701 agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca tggatcttat tgtcatacat
    38761 cactttattg atcggcgggg cattacgcag aaactgattt ttcagatcct gagtatattg
    38821 ccagagagct ttatcggtgg cgtagtcgtg tgttcccgga tagcgttttg ccagcacatc
    38881 gcccagacgc tgttcgttaa tcagtgttcg cacctgcgaa agcagctgct cgggataacc
    38941 ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa gagaaaaagg gtatactcac
    39001 gcaccctttt caggggcaac gtcgaaattt tatcattcag gagggccgat gagccactta
    39061 gacaacggtt tccgttcact gacactacaa cgttttccgg cgacggatga cgttaacccg
    39121 ctacaggcgt gggaagcggc agatgaatat ttgctgcaac agttggacga cacagaaatc
    39181 cccggcccgg tgttgatcct gaatgatgcc tttggtgcgt taagctgcgc gctggcggaa
    39241 cataagccgt acagcattgg cgactcatac atcagtgaac tggcgacgcg cgagaattta
    39301 cgcctaaacg ggattgatga gtcgagcgtg aagtttctcg acagcaccgc cgactacccg
    39361 caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac tggcattgct ggaacagcaa
    39421 ctgcgtgcgc tgcgcaaagt ggtcacgtcg gatacacgta ttattgccgg tgccaaagcc
    39481 cgtgacattc acacttccac gctggaactg ttcgaaaaag tgctcggccc gaccaccacc
    39541 acgctggcat ggaaaaaagc gcgcctgatt aattgcacgt tcaatgaacc gccgctggcc
    39601 gatgcgccgc agaccgttag ctggaagctg gaaggtactg actggactat ccataaccat
    39661 gcgaatgtct tctcccgcac cgggcttgat atcggcgcgc gcttctttat gcaacatctg
    39721 ccagagaatc tcgaagggga gattgtcgat ctcggttgcg gtaatggcgt tattggtctg
    39781 acgctgcttg ataaaaaccc gcaggcgaaa gtggtgtttg tcgatgaatc gccgatggcg
    39841 gttgcttcca gccgtttgaa cgttgaaacc aacatgccag aggcgttgga tcgcagcgag
    39901 tttatgatca acaacgcgct ctccggcgtg gagcctttcc gctttaatgc tgtgctctgc
    39961 aaccctccgt ttcaccaaca acatgcgctg accgataacg tcgcctggga gatgttccac
    40021 cacgcccgcc gctgcctgaa aatcaacggc gagctgtata tcgttgccaa ccgccatctg
    40081 gattacttcc ataaactgaa gaaaattttc ggcaactgca ccactattgc gacgaataat
    40141 aaatttgtgg tgctgaaagc ggtgaagctg ggacgtcgtc ggtaagattg ggagttacct
    40201 gccggatgcg gcgtaaacgc cttatccggc ctacgttcag cacgttaccc ccaggccgga
    40261 taagacgcgg caagcgtcgc atccggcaat gtctgctcag ttctggaatc gcgcttccat
    40321 aaaacaaaaa atccctgtca ccatcctcta cattctcttg tttagcgttt ttctacgttt
    40381 attcttccgt cacacagata aattcatccg ttgccaatct gtcaacggat gttatcatat
    40441 tgccaatttg tcaacggacg tgatgaagaa tgcacctgat aactcaaaaa gcattgaaag
    40501 atgctgcgga aaaacacccg caacataaaa cggagttggt ggctctgggg aacacgattg
    40561 ctaagggata tttcaaaaaa cctgagtcat taaaagcagt attcccatct ctggataact
    40621 tcaaatatct ggataagcat tatgttttca atgttggggg caatgaatta cgtgttgtag
    40681 caatggtctt ttttgaatcg caaaagtgct acatacgtga agttatgacg cataaagaat
    40741 acgatttctt taccgctgtt catcgtacta aggggaaaaa atgattgcga ttgccgacat
    40801 cttgcaagca ggagaaaagc taactgctgt ggcacctttt ctggcgggta ttcagaacga
    40861 ggaacaatac acccaggcgc tggaactggt agatcatctg ctgctcaacg atcctgaaaa
    40921 ccccttgctg gatctggtgt gtgccaaaat aaccgcgtgg gaagaatcag cgcccgaatt
    40981 tgcggaattt aatgccatgg ctcaagccat gcctggcggt atcgccgtga ttcgtaccct
    41041 tatggatcaa tatggtttaa ccctttccga tctgccggaa attggcagta aatctatggt
    41101 gtcacgcgtt ttgagcggga agaggaaatt aacgctggaa cacgctaaaa aattggcaac
    41161 gcgattcggc atttctcccg ccttgtttat tgattaacgc gacgtgccgg atgcgttgca
    41221 tccggcgctt caaataacga tttaaatctc cagcgccagc cgtgttccct gggcaattgc
    41281 ccgtcgtgcg tccagctcca tagccacatc gcagccgcca attaaatgca cagttttccc
    41341 gctatcaatc agcggttgcg ccagcgcgcg gtttggctct tgccctgcgc agatcaccac
    41401 attgtccact gctaatacct gcgtttcgcc gttgatcacc acatgcagcc cgtcatcgtc
    41461 aatcttctga taacttacgc ctgggatcat tttcacaccc cgcgagagca gggtggtgcg
    41521 atggatccag ccggtggttt tgcctaaccc ctgtcccggt ttgctggctt tgcgctggag
    41581 catcacaatt tgccgtgggc tacgcgggat ctgcattccc tgcgggctta agccaccagc
    41641 ctgttgtagg ctgctgtcga tcccccactc attacagaac ctggcgatat tctggctggt
    41701 ggattcgccc ggctgactta aatacatcgc cgtatcaaaa ccaatcccgc cacaaccgat
    41761 gatggcaacc ttgttgccta ccggcgcttt gtcgcgcagt acatcgagat aactcaatac
    41821 cttcggatga tcgatcccgt cgataggcgg aacacgcggc acgatcccac tggcgaggat
    41881 cgtttcatcg aacgcctgta actgatccgc cgtcacggtg tgattgagtt tcagcgtcac
    41941 gcccgtcact tcgatcatcc ggcggtagta gcgcagcgtt tcgtaaaact cctctttgcc
    42001 ggggatctgt ttggcgatat taaactgccc gccgatctcg ctgtgcgcat caaacaatgt
    42061 tacctgatgc ccacgcgccg ccgcgttaat ggcaaacgcc agcccagcag gtccagcgcc
    42121 gaccaccgcc agattttttt tctgcacggc gggaaggatt ggcattttgg tttcgtggca
    42181 ggcgcgagga ttcaccaggc acgaggtgac tttgccaacg aagatctgat cgaggcaggc
    42241 ctggttgcag ccaatacaag tgttgatctc atcggctcgc cctgattgcg cttttgacag
    42301 cagttccgca tccgcgagaa acggtcgcgc catcgatact atatcggcat cgccgcgcga
    42361 gagaatatcg tcggcaacct gcggatcgtt aatccggttg gtggttacca gctgcagcga
    42421 gacgtggcct ttcagtttgc gcgtgaccca gctaaatgcg ccgcgcggca cgggcgtggc
    42481 gatggtcgga atccgcgctt cgtgccagcc tattccggtg ttgataatgg tcgcgcccgc
    42541 cgcttcaatg gcctgcgcca gttctaccgt ttcggcaaaa gtgccgccgc cttcgaccag
    42601 gtcgagcatc gacaaccggt aaataataat gaagtcgttg ccgacgcgtt cgcgcaccgt
    42661 acgcactact tctacggcaa atcgcatccg gttgcggtaa tcgccgcccc actggtcact
    42721 acgctgattg gtgcgcagcg tcagaaattc gttgatcaaa tacccttcag agcccatcac
    42781 ttcgacgcca tcgtatccgg cctcccgcgc cagttgcgcg cagcgggcga aatcgtcgat
    42841 cagttgcagg atctcttcat gggtgagttc gtgaggaacg aaacggttga tgggggcctg
    42901 caatgcggac ggtgcgacca gatgcggttg gtagctgtag cgcccggtat gcaaaatttg
    42961 cagggcgatt ttgccgcctt cctgatgtac ggcttcggta atggtgcgat ggtgtgggat
    43021 ctggctggcg tcgttgagca ttgcgccacc ttccatgcca acgcctatta aatctggtgc
    43081 aatgccgccg ctgacaatca gcgccacgcc gtgacgggcg cgttcggcat aaaacgcagc
    43141 cagccgttca gcaccgtccg ggtattcctc cagcccggtg tgcattgaac ccatcaacac
    43201 gcggtttttt aacgtggtaa aacctaaatc cagcggggcg aacagcgacg ggtagctcat
    43261 aaaattgtcc agtatgtaaa ataattgtta tgtggtcgga tgagttctaa tttagccgtc
    43321 gatggagaaa agggaaaagg ggagtgcggg gtttgtgatg ggattcaaaa agaatacgcc
    43381 cggtggtaac gccggatgcg acgcttgccg tgttttatcc ggcttggggg attgcacatg
    43441 tagggcggat aaggcgttca cgccgcatcc gccagccgtt gcaaattact gcttacggaa
    43501 aaaatcgtta tacaacatat acaaatgagc ggcactccag gagaaatttg gtgcgccttg
    43561 ctgcgcgcct gtcagcgggt tgtaattctc ctgaattggg ccatcggcgg ttaatccttt
    43621 ggcgtgcctg aagaacgtat ccgccagctt cagggcatca tcacgataac cgtaacgctc
    43681 catccctttc agaccaaacc agaactgatc cacccatacg cgcccgcgcc agtagatatc
    43741 agcgccaaaa gccggattgg ttaacgccgc cgttcccagc ggaacaaagg tgttgaactc
    43801 tttaggatcg agcatcacct tcaccaccgc gtcggcattg gcctgcgttg ccgcaccgtt
    43861 aaacagcggc gaccagcctt ccggcccttt accgcgctca acaatcggtt tgcccgcgca
    43921 gccgttcgcc agtggtttat cttcaatacg tacgtcatag tagaactggg tagtcgggtc
    43981 gaacatacag gtgttgatgt agtccgcgag ctgctgtgcc aactggcgat agcgtttggc
    44041 ctcttctggc ttaccgagga tggttgccat ctccgccaga taatggttat cgctgtacat
    44101 atagctggcc tgatccaccg actcctgcaa tagcgagtag cccagcaacg ttccgtcctg
    44161 actgcggttt tcggcgaatt tcaccgtcca gtcgctacgt ttgccgccat tagcgacata
    44221 tttatccaac tgttctttgt cgataaaacc aaagacggcg gcatcatcac gccccgattc
    44281 ccacgacgca gcgacctgtg ccggaatttc cagactgtcg tactggcctt tctccaccac
    44341 gcgggcgtag ttgttcagcc cagactgcgt ctcttcttta tcgccttttt tcaccgtaaa
    44401 cagcatctcg ccgctctcgg tgttgtgggc tttgtcgcgg gtcgcgccat attccggcac
    44461 cccgttgccg ttatgatcgc ggttacgtaa ccaccagtcg tgataggcca ccagtttcgg
    44521 gtacatctct gccaaccagg ctttatcctg ggtgacgttg tatacttcca tcaccgacca
    44581 ggcggcaagg ctgggtttgg tattacgttc gttccagttg ccgccatcgc caccacgctc
    44641 ggggctaagg ttccacgcta tcaggtcggg gacaaagccc acatcctgcg gacgcacgct
    44701 gtcgccaggc tggatctgcc aggagaagac cgcgcggata ttctctttgg cgatatccgg
    44761 attgaaatgc gccatcgcaa acgcctgctt ccaggtatcc cacggccagg tctgattgcc
    44821 ggagaaccag cgtccggtca ccgacggcgt gacggtgtta taattcaccg caccgcccgg
    44881 tgagcgccag ttaccgttga gcgtttcgat cgctttcacc gcgacgcgcg tctgttccgg
    44941 cgtcgcatcc ggattggtta accctttctt caggtattct tcccagcgtt gctgcgaggc
    45001 ggtgagataa aacgccggac gcgccagaat atcgcggatc tgcatttgct ctttgctaac
    45061 ttcctgagcg gtcagcagat gggaataggt ggtgtagagc gtggtcgaac cgttgatatg
    45121 cgccttactg gtaaagcgat taccgttgat ttcagtctgc accggcaggg atttatgcac
    45181 ctgatattcc gattcgccgg aggtcagcag atcccaggtg gcgcgcactt tgccaaaggt
    45241 gactttcagg ccatcacggg tggcgctgat tttgcgctga tagtcagggt attcgccagc
    45301 aatggtttta tcggaaagcg gtttcccttc tttcgcttcc agtttttcca gcagttcgcc
    45361 atcccacacc agatccagcg gtttattgct gatgatttta gtttccagta gtgacgtgcg
    45421 cggcgtggcg aagcgcagag tcatttcgac ctgcacatct tttgctgtca gtttttgcac
    45481 cagcgcgccg ggaatactgt atgcctccag cgtgaagttg actttcttgc cgtcttgcca
    45541 gacagtcagg cggtcgaaat tgctggccat aaagttgatg tattcttccg tcagcagcgc
    45601 aacgcccgga aagccgccca tggtgttcgg gccgtctggc aacagatgac catgccaggc
    45661 tccgagatcg aaaaacggat taaaacgctg gtgatcgtcg taatcgtaat ccttcatgta
    45721 ctgcggcgca ccagtacggt taatcacgtt tttataattg tcggcgttag cggcatgagc
    45781 ggaaaaactt atcagcagag cgcaggttac tggcgttaaa atagttttga ttttcatgaa
    45841 attacagtcc ttattaccag tagaaatatt gcatcaggaa gatttggtcg ctgggatcgt
    45901 tagaggcatt tttcatgtag aagcggctat caaaagccgt ggcgaaacgt ccgccgccat
    45961 attcgtacca gataccaaac tgcatgaaag aggtggtttc atcgtcagcg tggtcagggc
    46021 gatgtttggc gtaggtgtaa gcggtggaga gataaacgcc ctttaacgct tcatacatgg
    46081 cgctcaccat catcccgctt tcacttcccg gtacgtccag cccgtcggca cgtccggtat
    46141 ggtgccaggc gcgagcggca aagttagggg taaacaggcc cgtgagatag agttgtcctg
    46201 tgccgtcggt aatttccaga ccattcatcc aggtgatgcc ttcggcaagg tcatactgga
    46261 tgtaaccgtt catccattgc cggataggtg tatttgtcgt cgtagcgatc gtacttgcca
    46321 aaatgtagcc aggctttgct ttcgtcatga tgtccggcag gtgtggcggt gacgctataa
    46381 cgaaagttgc cgcttaagtt ctgtaccttc agcgcggtca taatgtcgcg ggtattggga
    46441 atgacgtagc ccaaatcggg tgtgaagtcg ccccaccatt gcaggtcatc cagcgaggaa
    46501 tcctgacgca gactgagcat gatttcggtg ccatcattgg tacgataacc gccatagaaa
    46561 cggttaatcc caccttcgaa accaccccag ctatggtcag gcacctaggc attaccgtcg
    46621 tggtcggcct gcacggtcca gccttcgccg taaatcaggc cgaaccagga gccgtacttc
    46681 atctccatgc caccttcaat ataggtgccg tcgctgtatt tatgtttatt ctcaccctct
    46741 aaatccatat atccaccaag ccctaattcg ccgtaaaaac gaaatgaagg agaagatgtc
    46801 gaggtaattg cgggtgtttt tgtgtcgtca tgttctgccg gacgtgcttc ttctcctgcc
    46861 gcagaaaagg cgtagcaaaa cggcagagca agtaatgctc tgcatggtgc agtgattaat
    46921 ttcattgtgt taatccatga gctaaagaaa aagtacagcc ttttatcgtt gtaattataa
    46981 aaatataaag gcagaaacta ttttccctct cctcatccag gaagagggaa aatgcctgct
    47041 cattttttat tagtctgctt aatcaacatt gctggctggc gtcgcttcaa tatggcgctc
    47101 ttcagccgtt aatcccttta tatatttact gtatttccac cacgcaaagc cgaggaagat
    47161 aacaataccg ccgacgttat aaaaaatgat ggtcagaata ttcgcgccag tcgggaatgt
    47221 cgaagcgaca aaccctacgg caaatatcgc aatcagcatt gaaacaacga taataccggt
    47281 gcggcgggag cccatacgga aatcgcgtgg caggtgatct aatttggcgc gtaaattcag
    47341 gtaagccagc atgatgaata acggcggaag catggacgct gcggcggtca tattaataat
    47401 ggtattcatc agatcctgca cggtattgga accgagcatc gggataatca tcagcgggat
    47461 gacgatcaga aactggatcc atgctgcgcg cgccggaacg ccgttttcgt taagttcgac
    47521 ggttttctta ccaaagatgc cttccgggat ttcggagaag aaaattttca ccggcgttgc
    47581 ggtccacatc agcagggaac cgaacattgc ggtaaaggac accagaccga caaagcgatt
    47641 catcaacgct tccggcagac caaaatacgc cgccatgccg tggaatacct gcaccgatcc
    47701 gccggtaaat ttcaactctt tgctgctgac gaagacgtta atcagcaccg aggagacgga
    47761 atacagtacg ccgataaaaa tcccggcgag gatgatcact ttaacgaacg atttcgaacc
    47821 gcctttgacg tcgttaacgt acaccgcgac ggactccgcg ccacctgcgg ccataaagat
    47881 ccaggtggta acgccgagga acgcccagtt gaagttcggg atcatcgcgt caacggtgat
    47941 cggatcggca ggctgtacgt cgccaaccag tgccgtacct gccagtaaaa tgtaggagag
    48001 cgttaacagc agcatcagcg ttgaagtgac ggaggtaatt ggccccagca ttttcgcccc
    48061 gttggtggaa acccaggtgg agaaggcgaa cagcaccata ctgataacgg tggtggcaac
    48121 cggcgtcata atatattcgt agccgaggaa ggcatacgaa gcataagcaa taacgcgcgg
    48181 caacagcgag gtaaaaaaga acagatttac gaaccagtaa gtgtaggcag taataaatgc
    48241 ccaacgtccg cccagcgaac tttttaccca cgcgtagaca ccggcttctg agtttttatt
    48301 taacgaaaca aattctgcga tgatcagaca gaagggaata aaataaaaaa tcgtcgcgag
    48361 gaaaaacatc ggtgccgagg ccagtccaag ctcaatatta ttattgataa cgttgttaaa
    48421 gctgtaaacg gcggcaaaag tcagcgagag caagccgaat ttgccgattg tattacgttt
    48481 ggtatcagac ataacacatc tccgaaaatg tgtagggtag cggtggcggc aaactccttt
    48541 tgccgccgta gttgttattt gttatggaaa taaccatctt cgatggtgac tttgagaacc
    48601 acttttttga ccgcgttatt gcagataaac cgatacgctt catggatatc gcaaataacg
    48661 atttgccctt cgtggacctc tacggtttct ccgcagcctt ttaaatattc acggtcagtt
    48721 tcatcacgat aatattccac tacctgtaat gtctctttcg gcgcatattc aattttttgc
    48781 tgcccttgca ggtagtaatg cacttcaaaa tagcgtcgat gcccggtaaa tagcgcatcg
    48841 gtcgcggagt cattttccac acggtaggtc aatgaatcac cgatggagtg ggcgacgcca
    48901 ggctgaatgt tgtcgatatt ttcaatcgct tcaacgcagc gttgccactt cttgccagag
    48961 gcgtaaatct ggcggaactg ttctaagtta tcgatgatcc tcattgctta ttctcgctgt
    49021 gcaaattcgt ggaaaagaac cctgcgccga actcatacga cgccaggctt tgcgcggtag
    49081 cttctccgcc agaaaccggc agcaacgtaa agccgtagct gaagtcacgg aaccagacgc
    49141 gccaggagtc cagcacctcg ctgccccagg agttggagcc gaggccaagt agctggtggt
    49201 cgaggttcag ggtgatgtca tcactgcgct gcagctcgtt acagtgctgg gcagcgtgga
    49261 tgttttcctg ggtatagtgc caggcgctga agttaattgg gcgctgcgga accaccagca
    49321 gaccgttgtc gtggcggtta gtcagtgccg tccagcggac atgctgacgg ttgccgttgt
    49381 tctgcgggaa gggatagttc tcgaacatgg catcgacggt gctgcgccag atatcgatga
    49441 tgttagcctg ctggctgtcg gcgtagtttt cgcccggtcc acgaccgtaa tacgccacct
    49501 gatcgtattc gccgttaatc cccatggtga aaccgatgca cggaatgatg tgcggatagt
    49561 cgccgtaacg ctcgccggaa agcgccacgt taacctggcc atcggcagcg atgcgccaga
    49621 tgtaggtaca gcgcatcccg aagtcaaaca ccggcggtgc aataactgtg cggttgatga
    49681 tcaacacttc accatcgctc tgttctacgg caaagtcgcg cagatgttcc tgcatgatct
    49741 gcaaatgatt cggctgccac aacccttcgt actcctgctt gtggttgtcg atcatcggct
    49801 tgaagaagtt gatctttggc tcgcgggtca gcagcgactc gccatttacc tgccaggatg
    49861 tcggtttgcc actcattttt gagaaggtga tcgcgaagtt gtagccgcga acggtgcagc
    49921 tcaaacgatc gtcttccagc gtcagcggac gcgcattatt tggtgcgaaa ggcactggct
    49981 gcgcggtgtt ttccttcagc gggaactgat aagtggcgat agaatgcccg gcttcgctgt
    50041 agcgggtgcg ggaatctttg gtcaccgtaa tgttgaggaa cgcttcgcgg gcgtccagct
    50101 gcggcagcgt gatctgcaag ggggcttcgc tgttcggcgc aacgtcgcgc agtttaatct
    50161 gctgcgtcgc gagcgtttca ccttcggcgc gcacctctgc gtgcagggtg tagtcatcaa
    50221 gcgtggtaaa ccacagttta ttttcgactt tcaattcgcc gcgagtcagc gcgtggattt
    50281 ttaccggcgc gataacctgt ttgtactctt tcaggcccga tcccggcgtc tgatcggaat
    50341 agatcaaacc atcaagacag aagttatagt tgttgggata gtcgccgtag tcgccgccga
    50401 atttatacca gacattgccg tggtcgtcct gtgcctggat cccgtggtcg caccactccc
    50461 agacataatg accctgaatg caatcgtgct tatagaagac gttctggtac tccgtcagcc
    50521 cgcccggtcc gttccccatc gcatgagcat attcacagat gatgcgcggc ttcggatgcg
    50581 ggtattcacc aaactcattc atcagcggca cgcgggtgta catggtggaa ataatatcga
    50641 ccacttcagc atcgcgatct tcttcgtaat gcaccagtcg cgtgtcatcc agcgctttcg
    50701 ccgcatggta catcgcgcgg atgttacagc catagccgga ttcattgccc agcgaccaga
    50761 tgatgatcga cggatggttt ttctgtgcgt ggatatggcg aacaatgcgc tcgacgtaga
    50821 ctttttccca ctgcggatcg tcggtaatac ggctgatatc gccgacatta gcaaagccgt
    50881 gcgattcgac gtcggtttcc gccatcacaa acaggccgta gatatcacac aattcgtaaa
    50941 aacgcggatc gttcgggtag taagcggtac gcacggagtt gatattgtgc tgcttcatca
    51001 actggagatc tttctcgacg cgatccattc caacggcgcg gcctttgcga tgatcgttgt
    51061 cgtgacggtt gacgccgtgc agcatcacat aacggttatt gatccagaac agaccgtcgc
    51121 gcactttgat atcacggaag ccaacgcgtt gtggcaccac ttccagaacg ttgccgttgg
    51181 cgtctttcag cgtcatgacc agatggtaaa gataagggga ttctgctgac cattgctgcg
    51241 gctgttcgac agtaaaagca aagctggcgc tggtcagttt ttcaattgcc aaatgatcaa
    51301 tggcgctgct gtgcaccacg cgttcgccat caaacagggt atattccagc gtcgtgacga
    51361 caggggaggc ggcgagattt tccagcacca cttcgcagga aagcgtggca tcgcaatagg
    51421 cttcgtcaaa gtcggtacgc acagtgaaat cgttaatatg cgttaggtgt tttccgacca
    51481 gataaacatc gcggaagatc cccgccgacc accacatatc ctggtcttcc acgtaggtag
    51541 agtccgccca ctgcatcacg cgcacacaca acaggttgtc gccggtttta accatcgcgc
    51601 tgatgtcaaa ctctgcggtc aggcgactgc ccttgctgaa acccacatac tgaccgttaa
    51661 cgtagacttc aaaataggtt tcgacgccgt caaatttaat cagcgtctgt ttaccctgcc
    51721 agccgtcgct gagggtgaaa atacgttgat aggcaccggt tgggttatcg ctggggacaa
    51781 acggcacatc gatggggaac ggaaaacctt cgtcggtata ttgcagtttg ccgtgacctt
    51841 ccatttgcca catggcgggg acggtaatat gcccccagtc agccattaac tcagaggtga
    51901 aggcttctgg tacttgcagc ggatggtcaa aaaagtggaa attccactga ccgcttaagg
    51961 gcagaaacag gctgctggtt tcgcgggcaa aggtacgcgc ttgcgcaaca gaatcatatg
    52021 aaaaaaagta cgcacgcggc gcaagtcggt tttcgtgggt gagctgaatg ttttcccagc
    52081 gattcatagg gcctcccggt agagagaaat cggaaccatt catggtgatg gtagtttgtg
    52141 taattgctgg gggtttagta aaatttttac taaaagtagc aggtaaatgc atttacttta
    52201 actggatcac gaaaataaac ggcagcactg tccggcaaaa atgattgccg gacagtgtgt
    52261 aaggggattt aacgggtcgt gccgcgcagt tttaatttgc tgggaacgaa gactagcagc
    52321 ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt taacgccctg acttcccatc
    52381 atctcggaat ggatgcgcac ggtggagagc ggcggaaagg taaatcgcgc ggtggggata
    52441 tcgttaacgc tgataagcga aatatcttgt gggatgttca ggccacgttc atgaattgcc
    52501 cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca gtgccttcgg atagtcttcc
    52561 cgcgccagca tttgttttgc cagttcataa cccgacgaac tggaaaaacc gccgcgccag
    52621 atatcctctt cgcgtaccac ttgtttcagt cggccatatt ccgcaaaggc gacctcacga
    52681 atatccgcct tgccaggctc atcttcaccg ccaataaaac caatacgatt aacgccctgg
    52741 ttgatataga agtcgatgat ttctttactg atgcgtgcca gatcgatatc caccgcatcg
    52801 taaccgctgc cgggttcgtg aaagtcgata aaacagatat tgtcggtcaa cgcgctggca
    52861 gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa taccggtgac gtttttaatg
    52921 tctggtaatc cgttgtgttc ataacagttg ttcagttcga tcgccagctt ttcgcactga
    52981 gtttcaatgc cgtggcggat cgccagatag taaggatcgt tgatctccag ctcctgctgg
    53041 tagctgtaga tagccagaat atggtgttgg ttgactgcgc ctgtctgtag tttacgggcg
    53101 ctactggtct tgtactccag cttttcggcg atctcgagaa tgcgatgttt cgtctcttct
    53161 ttcacattca atgtcggatc gtcatttaag accctggata ctgtcgccag ggatacgcca
    53221 gcttcgattg cgatgtcttt taatgttgcc atgtttacct tttttacttt ccctgatgtg
    53281 cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt ttcagtaaat aaagcgcgtt
    53341 tctttgacgc tgtaacggta acacagcggc ttaggaatgt gatcacgcct gcattctcca
    53401 tttttagcct acaggattga gaaaaacttt tgctatagtt tagtaaaatt ttacccgaag
    53461 ggaggaagca atggaaaccg tggcttacgc tgattttgca cgtctggaaa tgcgcgtcgg
    53521 aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag ctgtacatcg tacaggttga
    53581 tgtggggaaa aaacactgca aaccgtgacc agcttggtgc cgtactacag cgaagaggag
    53641 ctgatgggga aaacggtggt ggtcttatgc aatctgcaaa aagcgaaaat gcgtggtgaa
    53701 acgtcggaat gcatgctgtt gtgtgcggaa accgatgacg gcagtgaaag cgtgttgtta
    53761 acgccggagc ggatgatgcc tgcgggcgta cgcgtcgtat aaaaagatcg gatggcgacg
    53821 tcgtatcgcc atccgatttg atattacgct tcttcgacac ttactcgcat ggccgccagc
    53881 gccttacgcg ccgctttgat caccagttca cactgttcaa tggtcagtgt cagtggcggt
    53941 tcaatgcgga tcgttttggc gttattgagc gttccggcca ccagtacgcg ctggcggaac
    54001 atctcgctgg caaagttata gccgatttcg ttatcaacaa actcaatcgc catcaacatc
    54061 cctttaccac gcgcttcctg taccagatcg ggatattccc gcgccagttg acggaaaccg
    54121 tccagcaaca cattgatggt cgccagcgcc gccgcacagg ccagcgggtt gccgccaaag
    54181 gtggtggtat gcaggaatgg gttgtcgaac agaactgaaa acacctcttc agtggcgatg
    54241 gtcgcgccaa tcggcatcac gccgccgccg agggcttttg ccagacacag aatgtccggc
    54301 tgaacgttct catgttcgca ggcaaacatc ttgcccgtgc gccccatgcc cgtttgtact
    54361 tcatcgagga tcatcagtgc gccgaactca tcgcacagct tacgcacggc ggtgagatag
    54421 cccggcggcg gcagaattac gccaccttcg ccctgaatcg gttcgaggat caccgcagcc
    54481 acatcatcac cggttttttt gcactcatta agagccgtgc gcatggcttc gatattgcca
    54541 aacggcacat gacggaaccc tggcagcagt ggcataaacg gtttgcggaa ggtcgatttc
    54601 gccgtggccg acagcgcgcc aagtgattta ccgtggaacg cgccgctggt ggcaataaaa
    54661 gtaaatttgc cgcgcggcga ctgataagcc tttgccagct tcagcgccgc ttcaacggac
    54721 tcggtgccgc tattacagaa gaagctgtat ttcagtttac cgggcgttag cgcagcaagg
    54781 gttttcgcca gcatagcccg tagcggatcg agtagctcct ggctgtgcag tggttgtttc
    54841 gcaagttgat tctgtacggc ggaaaccaca actggattac ggtgccccac gttgaaaatt
    54901 ccaaaacctc ccaggcagtc gataaactcc tgtccctggg tgtcgacaag cgtatttaaa
    54961 cctcccgctt gccactctac ggctccgtaa tccccgccgg cggtaacaga tttgcgatac
    55021 tctaaaaacc ccggattgac atgctctttg aagtattcaa tcacctctcg gttaagtgct
    55081 ttcatctcct catgatccag cgttcgcttc tcaatgagat tcagggcgtg ggcgctgcac
    55141 gctaaagccg atgcgctcga aggtaacctg ttcaaaatat gctccggagg ctcgcgtatc
    55201 acatgatacc gatttaagta ttgcagggat tgcgccactc cggctgcgat cgcgaaaatc
    55261 gggataaaca caaatgcggt tatcatttgt gcaatattta atgctatccg ttaacatttg
    55321 ttgttatttt gcacggtttt gtcgggtatg gatttgatga ttgctgcgca aatgcacgtt
    55381 ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt atagtaacag
    55441 agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat tgcgatctaa
    55501 atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg acaagaagtt
    55561 aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg tcacccagca
    55621 aaataccccg ctggcggacg ataccactct gatgttcact accgatctgc aaagctatat
    55681 cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag agttacaagg
    55741 gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg cggatatgtg
    55801 gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc gcaaaaatgg
    55861 cgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa aaatcagtgg
    55921 ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg agccgctgta
    55981 caaagcgttg aactccggac gtaccagtaa gcgtattcat aaaggcctgg tggtgcgtaa
    56041 aggctggctg ggtaaactgc cttcattacc gcttcgctgg cggacgcgtg gagtgatgac
    56101 cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg tggtgacgta
    56161 tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc agattgtgcg
    56221 cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc gtaatagtgt
    56281 tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag ggcagcttgg
    56341 cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg tcagaaatgg
    56401 cagtgagacg ctggcgaaag gcaccgatga actgaacgaa catacccagc agacagttga
    56461 taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga aacagaactc
    56521 tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg cagtgcaggg
    56581 tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca gtacccagcg
    56641 cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata ttctggccct
    56701 gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg cagtggtggc
    56761 aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata ttcgcaagct
    56821 gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg ccgccggacg
    56881 gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg cccagattag
    56941 ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag tggatgagct
    57001 taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg tgtcggcgat
    57061 ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt aatcggtatg
    57121 ccggatccgg cggttggagc acaatacctg atgcgatgct ggcgcatctt atcaggcctg
    57181 cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc cggcaattgc
    57241 accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa gcggcgcggt
    57301 cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg ttgacgcgta
    57361 aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc tggcgcagcg
    57421 ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt tcgcggtttt
    57481 cttccagcca ctgcgcgcct tgttcggtca gcgcaatctg cttcttacca ccttcctctt
    57541 cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgtcgggtag atgacgcccg
    57601 ggcttggggt gtaattcccc tgggttagat tctcaatcgc tttaatcaat tcgtaaccgt
    57661 ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg tgaccaaaga
    57721 aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcaacattga ccgtgttcat
    57781 gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag cagccctcat
    57841 ggcgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata tcagcctcct
    57901 tttgcttgat tagatatatc taatttatat ctgatgtgag cattactgca agtacaaaaa
    57961 tgatttttaa acgtggaagc acctcacatc taggccggat aagacgttca ctccgcatcc
    58021 ggcagcattg ctatggcact gaatgccttc acatacttca gacatatcag ttctacaaat
    58081 cgcttgcatt tatcatgatt aacaatcatt atcatttgcg aattttattt agatatatct
    58141 gattaccatc acgaaggcga taacaatgaa taacaccccc cgctacccgc agcgcgttcg
    58201 caatgatctg cgcttccgtg aactgactgt gctacgcgtt gagcgcatca gcgccggttt
    58261 tcagcgcatt gtccttggcg gcgaggcgtt ggacggtttt acatcgcgcg gctttgacga
    58321 tcacagcaaa ctcttctttc ctcaacctga cgctcacttt gtgccgccaa cggtaacgga
    58381 agagggcatc gtctggccgg aaggaccacg cccaccgtcg cgtgactata cgccactgta
    58441 tgacgaacta cgccatgaac tggcgatcga tttctttatt cacgacggtg gggtcgccag
    58501 cggctgggcg ataaacttac ggtggcaggt ccgcgcggtt cgctggtggt gccggaagat
    58561 tacgcgtatc agctgtatgt ctgcgatgaa tccggaatgc ccgcactgcg ccgccgcctg
    58621 gaaacgttga gcaaacttgc cgttaaaccg caagttagtg cgctggttag cgtgcgggat
    58681 aacgcctgtc aggattatct cgcgcacctt gatggtttta atatcgaatg gctggcacat
    58741 gatgagcagg cggtagatgc gcgtctggcg cagatgcaaa tccctgccag atgattactt
    58801 catctggata accggcgaag gtaaagtcgt taagaattta agccgccgct ttgaagcgga
    58861 acagtatgac ccacagcggg tacgtgcagc ggcttactgg cacgcaaagt aactgatata
    58921 taaaaagaaa aaggctgacg atttctcgtc agcctttgct atatctggtg gcccctgctg
    58981 gacttgaacc agcgaccaag cgattatgag tcgcctgctc taaccactga gctaaggggc
    59041 catggtagcg gattataaag taactccgtg tcgcaatcca gccattaccg cgcgcctgct
    59101 gtttttataa acaatgtatt ttcaatccgt tatacttttc ctggtgatgt tagaaaggag
    59161 taaatatggt tgaggatatt ttggctccag ggttacgggt cgtgttttgc ggtatcaacc
    59221 ctgggctttc atccgccggg actggttttc cctttgctca tccggcaaat cgcttctgga
    59281 aggtgatata tcaggccggg tttaccgacc gtcagttgaa gccgcaggag gcacagcatc
    59341 tgctggatta tcgttgtggc gtcaccaaac tggtagaccg tccaacggtg caagccaatg
    59401 aagtttcaaa gcaggagctg cacgcaggtg ggcgtaagct gattgaaaaa attgaagatt
    59461 atcagccgca ggcgttggcg attctgggca aacaagcata tgaacaggga ttcagccagc
    59521 gcggtgcaca gtgggggaaa caaacgctca ccattggttc gacgcagatt tgggtgctgc
    59581 caaatcccag cggtttaagt cgcgtttcac tggagaaact ggttgaagcg tatcgcgagc
    59641 tggaccaggc gctggtagtg cgtgggcgat aaaaaacgcc accga
//
LOCUS       DADLZM010000009        59523 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLZM010000009 DADLZM010000000
VERSION     DADLZM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.009"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNY73_000560"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNY73_000560"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY8046679.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNY73_000561"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNY73_000561"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY8046680.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNY73_000562"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNY73_000562"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY8046681.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNY73_000563"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNY73_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY8046682.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNY73_000564"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNY73_000564"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY8046683.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNY73_000565"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNY73_000565"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY8046684.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JNY73_000566"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JNY73_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY8046685.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWCGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNY73_000567"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNY73_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY8046686.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEMAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNY73_000568"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNY73_000568"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY8046687.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNY73_000569"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNY73_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY8046688.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JNY73_000570"
     CDS             complement(14847..15542)
                     /locus_tag="JNY73_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY8046689.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JNY73_000571"
     CDS             complement(15665..16702)
                     /locus_tag="JNY73_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8046690.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="JNY73_000572"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="JNY73_000572"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8046691.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17581..19269)
                     /gene="ggt"
                     /locus_tag="JNY73_000573"
     CDS             complement(17581..19269)
                     /gene="ggt"
                     /locus_tag="JNY73_000573"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY8046692.1"
                     /translation="MLLCSQEVVLAPPVSYGVEEDVFHPVRAKQGMVASVDATATQVG
                     VDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKNGNTTAIDFREMAPAK
                     ATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGTMPLNKVVQPAFKLAR
                     DGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKSGTLVQANLAKSLEMIAE
                     NGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPISGDYRGYQVYSMPPPS
                     SGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADRSEYLGDPDFVKVPWQ
                     ALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTTHYSVVDKDGNAVAVTYTL
                     NTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANAVGPNKRPLSSMSPTI
                     VVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNAPRFHHQWLPDELRVE
                     KGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGVSDPRSVDDLTAGY"
     gene            19423..19863
                     /locus_tag="JNY73_000574"
     CDS             19423..19863
                     /locus_tag="JNY73_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY8046693.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNY73_000575"
     CDS             complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNY73_000575"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY8046694.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20615..21502)
                     /gene="ugpC"
                     /locus_tag="JNY73_000576"
     CDS             complement(20615..21502)
                     /gene="ugpC"
                     /locus_tag="JNY73_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY8046695.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNY73_000577"
     CDS             complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNY73_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY8046696.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNY73_000578"
     CDS             complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNY73_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY8046697.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNY73_000579"
     CDS             complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNY73_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY8046698.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNY73_000580"
     CDS             complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNY73_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY8046699.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNY73_000581"
     CDS             complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNY73_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY8046700.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26601..27878)
                     /locus_tag="JNY73_000582"
     CDS             complement(26601..27878)
                     /locus_tag="JNY73_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY8046701.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNY73_000583"
     CDS             complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNY73_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY8046702.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28849..29958)
                     /gene="livk"
                     /locus_tag="JNY73_000584"
     CDS             complement(28849..29958)
                     /gene="livk"
                     /locus_tag="JNY73_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY8046703.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLACSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30382..30765
                     /gene="panM"
                     /locus_tag="JNY73_000585"
     CDS             30382..30765
                     /gene="panM"
                     /locus_tag="JNY73_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY8046704.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30964..32067)
                     /locus_tag="JNY73_000586"
     CDS             complement(30964..32067)
                     /locus_tag="JNY73_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY8046705.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNY73_000587"
     CDS             complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNY73_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY8046706.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNY73_000588"
     CDS             complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNY73_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY8046707.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNY73_000589"
     CDS             complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNY73_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY8046708.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="JNY73_000590"
     CDS             complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="JNY73_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY8046709.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36805..37401
                     /gene="rsmD"
                     /locus_tag="JNY73_000591"
     CDS             36805..37401
                     /gene="rsmD"
                     /locus_tag="JNY73_000591"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY8046710.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37391..37660
                     /locus_tag="JNY73_000592"
     CDS             37391..37660
                     /locus_tag="JNY73_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY8046711.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37663..38022)
                     /locus_tag="JNY73_000593"
     CDS             complement(37663..38022)
                     /locus_tag="JNY73_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY8046712.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38163..38789
                     /locus_tag="JNY73_000594"
     CDS             38163..38789
                     /locus_tag="JNY73_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY8046713.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38863..41061
                     /gene="zntA"
                     /locus_tag="JNY73_000595"
     CDS             38863..41061
                     /gene="zntA"
                     /locus_tag="JNY73_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY8046714.1"
                     /translation="MSTPDNHGKKAPQFAAFKPITTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="JNY73_000596"
     CDS             complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="JNY73_000596"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY8046715.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41629..42294
                     /locus_tag="JNY73_000597"
     CDS             41629..42294
                     /locus_tag="JNY73_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY8046716.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42367..42924
                     /gene="dcrB"
                     /locus_tag="JNY73_000598"
     CDS             42367..42924
                     /gene="dcrB"
                     /locus_tag="JNY73_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY8046717.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42928..44145)
                     /locus_tag="JNY73_000599"
                     /pseudo
     CDS             complement(42928..44145)
                     /locus_tag="JNY73_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44277..45326
                     /locus_tag="JNY73_000600"
     CDS             44277..45326
                     /locus_tag="JNY73_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY8046718.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLYLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTRVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45381..45968
                     /gene="acpT"
                     /locus_tag="JNY73_000601"
     CDS             45381..45968
                     /gene="acpT"
                     /locus_tag="JNY73_000601"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY8046719.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46079..47653
                     /gene="nikA"
                     /locus_tag="JNY73_000602"
     CDS             46079..47653
                     /gene="nikA"
                     /locus_tag="JNY73_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY8046720.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47653..48597
                     /gene="nikB"
                     /locus_tag="JNY73_000603"
     CDS             47653..48597
                     /gene="nikB"
                     /locus_tag="JNY73_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY8046721.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPTTL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48594..49427
                     /gene="nikC"
                     /locus_tag="JNY73_000604"
     CDS             48594..49427
                     /gene="nikC"
                     /locus_tag="JNY73_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY8046722.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49427..50191
                     /gene="nikD"
                     /locus_tag="JNY73_000605"
     CDS             49427..50191
                     /gene="nikD"
                     /locus_tag="JNY73_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY8046723.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50188..50994
                     /gene="nikE"
                     /locus_tag="JNY73_000606"
     CDS             50188..50994
                     /gene="nikE"
                     /locus_tag="JNY73_000606"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY8046724.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51000..51401
                     /gene="nikR"
                     /locus_tag="JNY73_000607"
     CDS             51000..51401
                     /gene="nikR"
                     /locus_tag="JNY73_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY8046725.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51521..51880)
                     /locus_tag="JNY73_000608"
     CDS             complement(51521..51880)
                     /locus_tag="JNY73_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY8046726.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51877..52152)
                     /locus_tag="JNY73_000609"
     CDS             complement(51877..52152)
                     /locus_tag="JNY73_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY8046727.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52211..53335)
                     /locus_tag="JNY73_000610"
     CDS             complement(52211..53335)
                     /locus_tag="JNY73_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY8046728.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="JNY73_000611"
     CDS             complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="JNY73_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY8046729.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQSFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQLTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56067..57134)
                     /locus_tag="JNY73_000612"
     CDS             complement(56067..57134)
                     /locus_tag="JNY73_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8046730.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57500..59122)
                     /locus_tag="JNY73_000613"
     CDS             complement(57500..59122)
                     /locus_tag="JNY73_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY8046731.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKSQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINLGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        14066 a        16425 c        15074 g        13958 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgtttgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggtgcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 tttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgaa cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatacgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 gcccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgccgctc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca
    19261 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19321 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19381 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19441 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19501 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19561 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19621 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19681 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19741 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc
    19801 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19861 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19921 gcaactctgc cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19981 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    20041 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    20101 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    20161 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20221 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20281 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20341 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20401 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20461 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20521 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20581 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20641 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20701 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20761 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20821 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20881 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20941 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    21001 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    21061 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    21121 ccggatcacg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21181 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21241 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21301 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21361 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21421 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21481 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21541 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21601 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21661 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21721 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21781 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21841 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21901 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21961 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    22021 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    22081 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    22141 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22201 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22261 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22321 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22381 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22441 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca
    22501 ccgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22561 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22621 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22681 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22741 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22801 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22861 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22921 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22981 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    23041 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    23101 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    23161 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23221 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23281 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23341 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23401 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23461 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23521 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23581 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23641 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23701 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23761 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23821 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23881 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23941 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    24001 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    24061 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    24121 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24181 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24241 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg
    24301 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24361 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24421 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24481 ggagcgttgc cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct
    24541 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24601 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24661 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24721 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24781 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24841 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24901 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24961 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    25021 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    25081 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    25141 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25201 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25261 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25321 ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc
    25381 agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga
    25441 acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac
    25501 tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag
    25561 aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc
    25621 cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg
    25681 cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc
    25741 tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg
    25801 ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg
    25861 gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg
    25921 attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa
    25981 caagatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt
    26041 ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac
    26101 catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac tcgcctgacg
    26161 gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc
    26221 gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag
    26281 ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac
    26341 atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag
    26401 atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt
    26461 aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta
    26521 cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt
    26581 aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc
    26641 agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc
    26701 aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc
    26761 accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact
    26821 atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga
    26881 cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcggtc
    26941 agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac
    27001 gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac
    27061 accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc
    27121 aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg
    27181 agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca
    27241 gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga
    27301 tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc
    27361 agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca
    27421 aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga
    27481 ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata
    27541 tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg
    27601 aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg
    27661 gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc
    27721 gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg
    27781 gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa gaacatcgcg
    27841 gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac
    27901 ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat
    27961 ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc aggatcagcc
    28021 cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt
    28081 tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta
    28141 cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg
    28201 tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc
    28261 ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc
    28321 agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc
    28381 actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt
    28441 agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt
    28501 tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga
    28561 ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca
    28621 gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga
    28681 agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg
    28741 tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca
    28801 taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc
    28861 cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc
    28921 tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt
    28981 caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac
    29041 tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc
    29101 atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat
    29161 gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttccggccc
    29221 cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg
    29281 gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc
    29341 ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc
    29401 tttcagcccg tcctgcaccg aacacgccag cccttcgcca tactgttgtt tgtcgtgaat
    29461 gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg
    29521 ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg
    29581 gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg
    29641 ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt
    29701 gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag
    29761 cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt
    29821 aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt
    29881 aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt
    29941 tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt
    30001 atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta
    30061 aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta
    30121 agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca
    30181 taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat
    30241 ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc
    30301 aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt
    30361 tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac
    30421 caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt
    30481 gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg
    30541 gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc
    30601 cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca
    30661 tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag
    30721 gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt
    30781 caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg
    30841 tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc
    30901 cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa
    30961 caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa
    31021 gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt
    31081 cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa
    31141 agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc
    31201 tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt
    31261 caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc
    31321 ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc
    31381 catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc
    31441 caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc
    31501 attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt
    31561 gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt
    31621 cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt
    31681 cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc
    31741 agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa tgccgtcgtt
    31801 aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac
    31861 gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc
    31921 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac
    31981 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
    32041 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
    32101 tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc cgtgctttag
    32161 cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt
    32221 ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag
    32281 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
    32341 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
    32401 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
    32461 ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag
    32521 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
    32581 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
    32641 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct
    32701 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
    32761 ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
    32821 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc
    32881 gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc
    32941 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
    33001 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
    33061 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
    33121 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
    33181 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
    33241 ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
    33301 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
    33361 aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga
    33421 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
    33481 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
    33541 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg
    33601 acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca
    33661 gcagcgcacc accgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt
    33721 tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga
    33781 cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc
    33841 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg tcaatgccgt
    33901 taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt
    33961 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
    34021 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
    34081 tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca
    34141 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat
    34201 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
    34261 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
    34321 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
    34381 tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc
    34441 gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
    34501 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc
    34561 aggttaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
    34621 agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc
    34681 agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg
    34741 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
    34801 gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg
    34861 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
    34921 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
    34981 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
    35041 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
    35101 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
    35161 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
    35221 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
    35281 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
    35341 cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag
    35401 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
    35461 tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
    35521 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
    35581 caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
    35641 caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
    35701 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
    35761 tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa
    35821 tctcacccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
    35881 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
    35941 cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga
    36001 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
    36061 gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct tcaaccggct
    36121 gttcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg
    36181 cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt
    36241 cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc tcttcggcgt
    36301 tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt
    36361 ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa
    36421 caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat
    36481 gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct tgcgcctgaa
    36541 cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc ggggtctgct
    36601 ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc gccatttgcg
    36661 actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat agcagctaaa
    36721 aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca agtatgtgtt
    36781 atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag cggccaaatc
    36841 cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag cccaggtctg
    36901 cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc ggtcattgtt
    36961 gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga agcgttatcg
    37021 cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca gcagttaatt
    37081 aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa cgcaatgtca
    37141 ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc gttccgccgt
    37201 ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc tgacgaagcc
    37261 ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc aaactggtca
    37321 ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca cgaagcacaa
    37381 ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt ggggattttt
    37441 aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta acgtggcgct
    37501 gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca ctttaccgaa
    37561 agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg gcgtgtttga
    37621 actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt cgccaacaaa
    37681 gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccacgg tcagggcgtg
    37741 gtactgctgg gcgtcgagac gaaacgtctg ctccattcct ccgctttgcg gtttgaagct
    37801 tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg aacggcgatc
    37861 gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga gcggagccat
    37921 atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg cgacgacaat
    37981 taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc ctcatcggaa
    38041 aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac cctcgctact
    38101 aaactggaag caagaccgta ggcattccgc ttacgaaaaa ataacgaatt caaggaacta
    38161 agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg gatgcatcgt
    38221 atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt ctcctgctgt
    38281 tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg gcgggactgt
    38341 gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg atgtacgcca
    38401 tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt cagatgacgc
    38461 tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg ctactggcga
    38521 ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc ggcatgacgc
    38581 tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct ccggcgctct
    38641 ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg gggagccact
    38701 atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt gccggtcact
    38761 tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg actccagagt
    38821 gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac acctgacaat
    38881 cacggcaaga aagcccctca atttgctgcg ttcaaaccga taaccacggt acagaacgcc
    38941 aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga aaacgtctcc
    39001 ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc gcgcaaggta
    39061 gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt cgccaccgaa
    39121 aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc ggtgcaaaaa
    39181 gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc gcgcctgaaa
    39241 gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg tctggagcag
    39301 ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg gctgtacccg
    39361 attgctcatc aggcattacg gctgatcaaa tccggcagct acttcgccat tgaaacctta
    39421 atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc tgcgatggtg
    39481 ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg cgcgcgtcag
    39541 ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg taacggtgag
    39601 cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt cgccgcaggt
    39661 gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga tgaaagcgcc
    39721 ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc tgcaggagcc
    39781 accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc cagcgccatt
    39841 gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat tgagcggttt
    39901 atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct gctggtaacg
    39961 ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa aggtctgacg
    40021 ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc gataacctcc
    40081 gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc gctggaacaa
    40141 ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt cggtaaaccg
    40201 cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct gacactggcg
    40261 gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg cgaagcacag
    40321 gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg gtctggcatt
    40381 gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat
    40441 gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt gctggtagta
    40501 cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc cgacgctaca
    40561 actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac cggcgataat
    40621 ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc gggcctattg
    40681 ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc
    40741 ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat tgctatgggt
    40801 agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa ccacctgcgc
    40861 ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg ccagaacatc
    40921 accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg gatgaccggg
    40981 ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc gaatgcgtta
    41041 aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc
    41101 gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc aatcagcccc
    41161 tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc tttcgcaacc
    41221 agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg
    41281 gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg caccatcatc
    41341 accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga
    41401 tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct gtgagtccgt
    41461 aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt
    41521 atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa cagacgtgtc
    41581 gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat gaacgttttc
    41641 tcgcaaactc aacgctataa ggcgttgttc tggttatcgt tatttcatct tctggtgatc
    41701 acctccagta actatctggt tcagcttccc gtctccattt ttggtttcca taccacctgg
    41761 ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg tatttttggc
    41821 gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac
    41881 gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact cgcccacttc
    41941 aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc
    42001 ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca
    42061 gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat tgccttctgg
    42121 cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt cgattactgt
    42181 ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt actcaatatg
    42241 ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc
    42301 gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat cgaaaggaag
    42361 aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat ggggcttgcg
    42421 gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggtcgcgga aagtaacgct
    42481 accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc agcggatatg
    42541 accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg gtccgacgcc
    42601 accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg
    42661 ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca agtggtaacc
    42721 aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat tatctccgcg
    42781 aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca actgctgacc
    42841 atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc
    42901 attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag ggacgtgttc
    42961 cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca gtcccgccgc
    43021 cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca gtggtccagt
    43081 cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc cctaattttg
    43141 ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg aaaacccggc
    43201 ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc caaccagtag
    43261 caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac cgatacggtt
    43321 aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc
    43381 gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg tggcgatgac
    43441 gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc gcccaagcac
    43501 cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg ggatcgccaa
    43561 caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca agccgcccca
    43621 gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag tgacaatgcc
    43681 gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc cccatagggt
    43741 cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa
    43801 taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc ccagaccgct
    43861 caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca aatcggcgta
    43921 acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc
    43981 tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata cagcgagcgg
    44041 caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt
    44101 caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga tcaccacatt
    44161 tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga
    44221 atattgctgg cgtgactccc gctacactta acacaaaaag tcacaaggaa gccccaatgg
    44281 aaacccctca acccgataaa acgggcatgc acattctgct caagttggcc tcgctggtag
    44341 tgatcctcgc gggcattcac gcagcggcag atatcattgt gcagctgtta ctggcgctgt
    44401 tttttgccat cgtcctcaac ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc
    44461 ccgttgccat tacgattgta gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg
    44521 tactggcggc atcgtttaac gaatttatct ctatgctgcc gaagtttaat aaggagctga
    44581 cgcgcaaact ttttaaattg caggagatgt tgccttttct ttatttgcat atgtcgccgg
    44641 agcgaatgct gcagcggatg gactcggaaa aagtggttac cttcaccaca gcgctaatga
    44701 ccgggctttc cggggcaatg gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc
    44761 tgtttgaagt gcgccacgtc ccttacaaaa tgcgttttgc gctgaataac ccacagattc
    44821 acatcgcagg attacaccgc gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc
    44881 tactcagttt atggacaggt gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt
    44941 ttgcgctgat gtgggcagta ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg
    45001 taatttccgc cgtaccgcca atgattcagg tgctgctgtt taatggtgtt tacgaatgta
    45061 ttctggtcgg cgcattgttt ttagtggtcc atatggtcat cggcaatatt ttagaaccac
    45121 ggatgatggg ccatcgcctg gggatgtcca ccatggtggt atttctttca ttgttaatct
    45181 ggggatggct gctcggcccg gtagggatgc tactttcggt gccattaacc agagtgtgta
    45241 aaatctggat ggaaacgacg aaaggtggca gcaaactggc gattttactg gggccggaca
    45301 gaccgaaaag tcggttaccg ggatgaggcg acaagtgata cgatacgcac tttcattttc
    45361 cattaaacgt tggccctgat atgtatcgga tagttctggg gaaagtttcg accttaagcg
    45421 cagctccact gccaccgggt ttacgcgagc aagcaccgca aggtccacga cgcgaacgct
    45481 ggctggcggg gcgtgcattg ctttcgcaca cgctttcccc gctaccggag atcatctatg
    45541 gcgaacaagg caaacctgca ttcgcgccgg aaacgccgct atggttcaac ttaagccata
    45601 gcggtgacga tatcgctctg ctgttgagcg atgaaggcga agtcggctgc gatatcgaag
    45661 tgattcgccc gcgcgccaac tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac
    45721 acgctgaaat ggacgccgtg catcctgatc agcaactgga aatgttctgg cgcatctgga
    45781 cgcgcaaaga agccatcgtt aaacagcgtg gcggcagcgc ctggcaaatc gtcagcgtag
    45841 atagcaccta tcactcctcg ctgtcagtca gccattgtca gctagaaaat ttaagccttg
    45901 cgatctgcac ccccaccccc tttacgctca ccgccgacag tgtgcaatgg atcgattcag
    45961 ttaactgatc cgcccacccg actgcccatc tattgatcca gaacaggtaa tcagtatgac
    46021 gaatacttaa aatcgtcata cttatttccg ccatctattt taatccattg gggttaccat
    46081 gctctccaca ctccgccgca ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca
    46141 tgccgctgca ccagatgaaa tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc
    46201 gcacctttac acgcctaacc agatgttcgc ccagagcatg gtttatgaac cattggtgaa
    46261 atatcaggca gacggttcgg tgatcccgtg gctggcaaaa agctggactc attcagaaga
    46321 tggtaaaacc tggaccttca ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt
    46381 cgatgccgag gcggcggcag aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc
    46441 ctggctggag ctggcaaacc agattgttga tgttaaagca ctcaataaaa cagagctgca
    46501 aattaccctg aaaagcgcct actatccttt cctgcaagaa ctggccctgc cccgtccttt
    46561 ccgttttatc gctccctcgc agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc
    46621 gattggcacc ggaccgtgga ttttgcagga atcgaaactg aatcagtacg atgtcttcgt
    46681 ccgtaacgaa aactactggg gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat
    46741 cccggacccg actacccgcg cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg
    46801 aaacgaaggg ttattaccgc tcgatacctt cgcccgcttt agccaaaatc cggcttacca
    46861 cacccaactg tcacagccgc tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc
    46921 caccaacgag ctggcagtac gtgaagctct taattacgcg gtaaacaaaa aatcgctgat
    46981 tgataacgcg ttatatggca cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc
    47041 ttacgctaat ctcggcctga aaccgcgcca gtacgatccg cagaaagcga aagagttgct
    47101 ggaaaaagcc ggttggacgc tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc
    47161 gctgcgcatt gaactgtcgt tcatcggcac cgatgcgtta agcaaatcga tggcggaaat
    47221 cattcaggct gatatgcgcc agattggcgc agatgtctcg ctgattggcg aagaagagag
    47281 cagtatctat gctcgtcagc gcgacggtcg ttttggcatg attttccacc gcacctgggg
    47341 cgcgccatac gatccacacg ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt
    47401 ccaggcacag caaggattag ccgacaagcc gctgattgat aaagagatcg gcgaagtgct
    47461 ggcgacccat gacgaaacgc aacgtcaggc gctgtatcgc gacattctga cccgtctgca
    47521 tgacgaggcg gtttatctgc ctatcagcta catctcgatg atggtggtat caaaaccgga
    47581 gctgggtaat atcccctacg cgccgatcgc caccgaaatt ccgttcgaac agattaaacc
    47641 ggtgaaacct taatgttgcg ttacgtatta cgccgctttc tgctgctgat cccgatggtg
    47701 ctcgccgcct cggtgatcat ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc
    47761 gattatctgc gtttatctaa cctgccgccg acgccggaga tgctggcctc tacccgcacc
    47821 atgctcgggc tggatcagcc gctgtacgtc cagtacggta cctggttgtg gaaggcgctg
    47881 catcttgact ttggtatctc attcgccagc caacgcccgg tactggacga tatgctgaac
    47941 ttcctgccca ccacgctgga acttgcaggt gcggcgctgg tattaattct gctcacttcc
    48001 gtaccgctcg gtatctgggc ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc
    48061 tttatcgcgt ttcttggcgt gtcgatgccc aacttctggc tggcattttt gctagtgatg
    48121 gcgttttcgg tgtatctgca atggctaccc gcgatgggct acggcagctg gcagcacatc
    48181 attttacctg cggtttccat cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc
    48241 gccagtatgc tggacgtcgc cggtcagcgt cacgtcacct gggcgcggct acgcggcctg
    48301 aatgacaaac agaccgaacg ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc
    48361 gccgtgggga tgcacattgg cgaattgatt ggcgggacga tgattatcga aaacatcttt
    48421 gcctggcctg gcgtcgggcg ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg
    48481 attcagtgct ttacgctgat gatggtggtg gtttttgtgg tctgtaattt gattgtcgat
    48541 ttgctcaacg ccgcgctgga cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact
    48601 ttttcctctc ttcccgctgg tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc
    48661 tgattgcgct caccagccag tggtggctgc cgtacgatcc acaggcaatt gatttgccgt
    48721 cgtgcctgct ttcgccggat gcgcagcact ggctgggcac cgatcactta ggtcgcgata
    48781 ttttctcgcg gctgatggca gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc
    48841 tgctggtgct gacattaggg ctggttattg gcggcagcgc cgggttgatt ggcgggcgcg
    48901 ttgatcaggc caccatgcgc gtcgccgata tgtttatgac cttcccgacc tcgattctgt
    48961 cgttctttat ggttggcgtg ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc
    49021 tgtcgcactg ggcgtggtat gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac
    49081 gcgagtttgt gctggcgtca cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc
    49141 atctggcagg cgcggtgatc ccttcgctgc tggtgctggc aacgctggat atcggccata
    49201 tgatgctgca cgtcgcgggg atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg
    49261 aatggggcgt gatgattaac gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt
    49321 tctggccggg gctggcgctg tttatcagcg tgatggcctt taacctggtg ggtgacgcac
    49381 tgcgcgatca tctggatcct catctggtga cggagcacgc acactaatgc cgcaacagat
    49441 tgaactacgt aatatcgcgc tacaggccgc gcagccgctg gtacacggtg tatcgttaac
    49501 cctgcaacgc gggcgcgtgc tggcgttagt cggcggtagc ggcagcggga aatcattaac
    49561 ctgcgccgcg acgctgggca ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt
    49621 agccgatggc aaaccggttt cgccttacgc cctgcgcggc atcaaaattg ccaccatcat
    49681 gcagaacccg cgcagcgcct ttaatccact gcacaccatg cacacccacg cgcgggaaac
    49741 ctgcctggcg ttagggaaac ccgccgatga cgctacgctt accgctgcca tagaagcggt
    49801 ggggctggaa aacgccgcgc gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat
    49861 gttgcagcgc atgatgattg cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga
    49921 tgaaccgacc accgacctcg acgtggtagc acaggcgcgc atcctcgatc tgctggaaag
    49981 cattatgcaa aaacaagcgc cgggaatgct gctggtcacc catgatatgg gcgttgtggc
    50041 gcgtctggcg gatgacgtgg cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt
    50101 agaaacgctg tttaacgccc ccaaacatac ggtgacgcgc agcctggttt ccgctcatct
    50161 cgccctctac ggtatggagc tggcatcatg accttactta acgtctgcgg cctttcccat
    50221 cactatgccc acggtggatt tagcggaaaa catcaacatc aggcggtgct gaataacgtt
    50281 tccctgaccc tgaaaagcgg cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa
    50341 agcaccctcg cgcggctgct ggtaggctta gagtctccct cacaagggaa tattagctgg
    50401 cgcggcgaat cgctggcgaa acttaatcgc gcccaacgta aagccttccg ccgtgatatt
    50461 cagatggtgt ttcaggactc catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc
    50521 ctgcgtgaac cgatgcgcca cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc
    50581 agcgaaatgc tgaaggcggt cgatctcgat gacagcgttc tcgacaaacg cccaccgcag
    50641 ttaagcggcg gccagctcca gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa
    50701 ttgctgattc tggatgaagc cgtttctaac cttgatctcg tgttacaggc gggcgtcatt
    50761 cgcctgctga aaaatctaca acaacagttt ggcactgcct gcctgttcat cactcatgac
    50821 ttacgcctgg tggaacgctt ttgccagcga gtgatggtga tggacaacgg acaaatcgcc
    50881 gaaacccagg cggtgggaga taaattaacc ttttcctctg acgccggacg tgtgctacaa
    50941 aacgcggtat tacccgcatt ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa
    51001 tgcaacgagt caccatcacg cttgatgacg atttactgga gacgctggac agcctgagcc
    51061 agcgtcgtgg ttataacaac cgttccgaag ctatccgcga tattctgcgt agtgccctgg
    51121 cgcaggaagc tacccagcag cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg
    51181 aacacgaaaa acgcgactta gccagccgta ttgtttccac ccagcatcat caccacgacc
    51241 tctccgtcgc cacgctacat gtgcacatca accacgacga ctgtctggaa atcgccgtgt
    51301 tgaaaggtga catgggtgac gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg
    51361 tgcggcacgg gcatttgcag tgcctgccga aggaagattg agttatttct aaaacaagga
    51421 aagaaaagat attaactgaa cttaatttat aaaattaagt tcagttgcat agatagatcc
    51481 tgtaaaggca gatattctgc gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct
    51541 cattcagtgt ttcaataata taggtattaa cagagacttg ttgctgcgca gccgcattat
    51601 taaggcgttc actcagcgat tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac
    51661 gcgcatacgg ctcaataccc gcagctttac aatcctcaag atactctcgc agggaaagct
    51721 ctccttcttt ttgcagcccc tgaatactgt cagagacaaa atcacaataa cctgacaagc
    51781 ccaggaattt gccacgaaat gcattcagtt ctggcacgta ggtaataaca gctggttgcc
    51841 cggcaatctc catagagttt ggcgtcttca gtttgatcat ggttttaccc ctatacttag
    51901 taaccagtca cgcacgcttt ctacagcgcc tttatcggta tctggcgacg gatgaggccg
    51961 atggaaacac gcaacgctca tatttagtat gaacttgcaa cgcgaacctc ttccttcctt
    52021 aatttctccg cctaatgctt taaccagtga ctctatatca gaccacttga tcccttgagg
    52081 aacaggtgtt ttaaatattt gatccaatgt atttttttgt ttcttccgta aagcctgtac
    52141 cttggttctc atggcatgtc cttatgactt cactttatga catcacagta tgaagtcaag
    52201 tttcccttcc ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga
    52261 aaaacgcgcc gccaattgcc atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg
    52321 caccccggta gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca
    52381 taatgtcctg caccatctgc ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt
    52441 gcagcggcag cagcaccaga atcaccagca gccccagttg cggcattgaa cgcgctatcg
    52501 tccccataaa aatgccgatt gacgtggtgg caaacagact gagcgccacg cccagcataa
    52561 acagcgggat cgagccttca atcggtacgc ccagtacacc tttcaccatc agcaccagcg
    52621 ataatcccga taccaccagc accaccagcc ccatcgacca gatcttcgcc atcatgatct
    52681 caaacggcgt tatcggcatc accagtaaat gttccaccgt gccgtgttca cgctcgcgga
    52741 tcagcgccga tccggtcaat acaatcgcca gcatggtaat gttgttgatg atcgccatca
    52801 ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg catccgggtt tccagcgata
    52861 ccaacggttc gctgttatca cggtagcgcg cgacaaagct gttcacttca ccgttgataa
    52921 tattctggat atacccattg ccggtaaatg cctggctcat gcgcgtggca tcgacgttca
    52981 cctgaatatc cggctggcgt ccggcgagga catcacgctg aaaattaggc ggaatattta
    53041 tcgcgaaggt atagcgtccg gcgtccagtc cggcatccat ctcatcggcg gtgatcatct
    53101 ccggtggcaa aaaccacgga cgatagaagc tgttaacgat ccggttcgat aactgcgatt
    53161 gatccatatc ggcaatggcg atcggcgcga ggttcaacga tcctggcgta acggtcgctg
    53221 acgaatacac cgacaccgta aacgagaaga caatcagcgt cagcatcgct ttatcaccga
    53281 gcagactgcg caactctttg atacccagat taaaaatatt gcgtaaatgg cgcatcatcc
    53341 ctcctgtttt ttcagcagca ggatgcttaa gcccatcacc agcgggatgg ctatcagcaa
    53401 cgggataaaa agttgccaca aatcagtcag atccagcgct ttcgagaacg ttccgcgggc
    53461 gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc caacgtccag gcccttccag
    53521 cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc
    53581 cgttccgaaa atggcggcaa tctggctttt cataaaggtg gagatcagca gccccattcc
    53641 agtggcaatg atgatataca gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg
    53701 cggtacgcca aacacaaaca ccgacaggcc gcagagcagg aaaaagttca gcatccccag
    53761 cgcgatgtac ggcagctgtt tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta
    53821 aaggttgata atcgacccta gctctttttc ccgcaccacg ctaagggcgc ttagcattga
    53881 cgggatcatc atcagcagaa gcgggatcac cgccggaaca atcgctggca ggctttttac
    53941 gtccgggtta tagcgataac gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt
    54001 caattgtcgg ctcgccacat cctgaagcca actctggtgc attgcctgca cgtaaccttt
    54061 taccgtttcg gcacgactgg gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt
    54121 accacgcgcg atatcgcgcc cgaaattggg cgggatctca atggccaccg tgatatcgcc
    54181 cgcacgcatc cgacgatcaa gctcgtcata actggtgagc ggcggctgtt cgataaagta
    54241 acgggaaccg gagaggttga gtgtccacgc ctggctactg acggtctggt cgcggtcgag
    54301 caccgcaaag cgcaggtttt ccacatccat actgatgccg taacccatta tcagcatcag
    54361 gatcaccgtt cccatcagcg ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc
    54421 ttcgcggcgg ctgtagctaa acagacggcg caggctaaat ccctgacgcg gcgcgtgggt
    54481 ggtgtcgtgt accacgggcg gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa
    54541 ataggcgata aatgcctctt ccagactggc ggctccgcgt ttctcaacca gttcctgcgg
    54601 tgtaccgctg gcaagcactt ttccggcgtg catcagtgag atgcggtcgc aacgttccgc
    54661 ttcgttcata aagtgggtgg agatgaagat agtcactttg tcctggcgcg agagatcgac
    54721 catcaactgc cagaacatat ccctcgccac cggatcgaca ccagaagtag gctcatcgag
    54781 gattaacatc tccgggcgat gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc
    54841 gagcggcaat gactccggca gaacatcttc aacgtcgttg agcttaaaac gctcgctcat
    54901 ttcagccact cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg catgtaactc
    54961 caggttttgc cgcacggtga gttcgttata gagcgaaaac gactgcgaca tatagcccac
    55021 acgacggcgg gtatcgatat cttttggatc aaccggttgc ccaaatagcc acgcctcacc
    55081 ttcgctggcg ggcagcagtc cggtaagcat tttcatggtg gtggatttac cgcagccgtt
    55141 cgaaccaagg aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt gatcaacggc
    55201 aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc
    55261 aggttgatac ggtgggatca ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag
    55321 attaataaat gcttcttcca gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct
    55381 gccggttgcc agcacttctc cggcattcat cgctaccagc cagtcgaagc gttcggcctc
    55441 ttccatatag gcggtggcga ccagcacgct catattgctc tgccgctggc gaatactgtc
    55501 gatcagatcc cagaactggg cgcgggagag cgggtcaacc cccgttgttg gctcatcaag
    55561 gatcaacagt tccgggtcgt ggattaacgc gcagcacagc ccaagttttt gcttcatccc
    55621 gccggagagt ttacctgccg gacgatcgcg aaacggtgct aacccggtgc tggtcagcag
    55681 ctcattaatt cgcacttccc gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc
    55741 gacattttca tacaccgaca aggtgtggta gaggtttttg cccagcccct gcggcatcca
    55801 ggcgatgcgc gggcagacgt ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac
    55861 caccacattg ccctgctcaa tgacgcgggc accggaaatc agtgacaata aactcgactt
    55921 cccgacgcca tccggaccaa tcagcccgac catgcaacgg gccggaatat cgagagtgat
    55981 attgttcagc gcaacggttt ttccataatg ctggctcacg cccgccagtt gcgcgacagg
    56041 cgggacagga accagttcca gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc
    56101 cacggaagtt cttcattcac ccgcacccac gctacgcccg gtaaaccggt tttgacatat
    56161 tccagatgct gctggagtaa ttccggtggg atacgcgctt tgacgcggaa catcagtttc
    56221 agccgttcat cgctggtttc gacggttttt ggcgtgaact gggcgacact ggcgacaaaa
    56281 ctgatggttg caggaatacg cagatctggc gcggcatcga ggatcagccg ggcttcaccg
    56341 cccagtttca gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg
    56401 agatcgacca tattcagcac ccgaccgcct gccgccagca cttcccctgg ctcggcaacc
    56461 cgatactgca cgcgtccgtc acgcggggct ttcagttcac tgtcatcgat atctgcggca
    56521 atgcgccgtt cagtggcttg cgctgcttcg acccgagttt gcgcctgaat gatattggtg
    56581 cgtgctgctt ctatagccgc tttagaagcc gaaacctggg ctttcgctga ttccagcgca
    56641 gctcgggcgc tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga aatcgcccct
    56701 cgttgggcga gcgagcggga acgcgtatga cgtttggcaa cagagtccag ttcggcctgg
    56761 cgttgattga ccagcgactg tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc
    56821 tgcgcggcgg caacggcgct ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt
    56881 tcctgcaaca cgcgagtatc catcttcgcc agcacttcgc cttcgcgaac aaactggcct
    56941 tctttcacca gaatggtgtc gatacgcccg gcagttttgc tggcgatatc cacttccgtc
    57001 gcttcaatgc gcccattgct gacagcaaag ccttccggca cacccgccgg gcgcaacagc
    57061 caccacgcca cgacggcagc caccgccagt gccccgacaa cccaccacgc cagatggcgc
    57121 ttactcttat ccataatcga cccgccataa tccctgtaag caaacgacgc gtgttacaac
    57181 tgccgccgaa cgacgttcag cggattcttc catccgggat aagaaggaaa cgacaaatac
    57241 gaccagcgcg cagagcattg agcgccaacg gggaaggctt agcgaaaacg ggtagtaatg
    57301 ttgtcatcat cagcattcct ggccgtaaat gaaacacgca tactcataag tgtgatctat
    57361 aaaactggct gacaaaacgg tatccatcct gttggatcgc gtttcacgcc tgttcagttg
    57421 caggaacaga gcattccttt ttacgtttta tcaacagtct caaccagata gtaacattaa
    57481 tattcatgtt gtctatggtt cagagctgta acattgtgcg ccttctaatc aaaggcataa
    57541 gtccatttct gtttttttca tgctggtaag acttccaggg agtggttatc tctggccctg
    57601 caccaaggtt aatttggcaa ttatgcatgc tgaacattct tccagcatgt ttactgtcac
    57661 gggcgttaag acctaaaact gtccccgttt cacctatgcg ttctctccca ccatttccgt
    57721 tatgtgcaac aacttgattt atacctaaca tttgtgaaca tgctaatagt gcttcaagtt
    57781 taggtctgta atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa gagatagccc
    57841 ggctagtttt aaactttttg ccattaatta tagcatgatg ctttttatta ataatttccg
    57901 ctaattcgac cggatcgaga gcattggtca tttcatcaat agagacagtt atcggtccta
    57961 taatgtaacg ttttcgctga tcatcaaaag ttatcccatg atgatttgcc attattttgt
    58021 atttggggtc atagaaacat acaggataat cttttatccc gtcgtaggta tcctcatcaa
    58081 gtggtttcat gcgcgtgaag ttttgcatat agttaccatt gcaattcgtt tcatgatttc
    58141 cagctaatac atgaacattt tcattgatac tcattatgcg ttttaacaaa tcgatgataa
    58201 attgaacgcc acagatatta ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg
    58261 gcgtgactgc gtctttatta ataaccaaat aaggctgaag actttctaaa acattatctc
    58321 ttctgttcag tatactgatt attgtattaa cattagtgtc aagttgctca ttaaagttct
    58381 cccttagcaa tacgttgaga acttcatctt ctatattaag caactggcta aggaaatttt
    58441 ctccttcagc catgattccc aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga
    58501 ctgagctatc ggtatcgcca aaataggtcg gcctggtact tgtgggatca acaggtgaac
    58561 aattggagaa ataggttctg tcagcgaact gggattttat aattgtttcg aatttaatat
    58621 caaatacatt gtattgcgat atttcccagt tccagatggg tttgtacgac tcagtaactg
    58681 caacatcatt agcattatca tttactgtca tattattaag taaataaaca atgctgcacc
    58741 aattaatatc ggtattatca agagggcaac cgtgattttt actattaatt gtcacaataa
    58801 actgataaca gttattccta cgtagaatat gagcgcgcat gcccgatagc atactatatt
    58861 tttctgaatg attcgtttgt atggtaaatg aaattttgtc atcttgaata gattgttgga
    58921 aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt gtcctggcaa
    58981 tacttgatat atcatcacct ataccatttg caatataccc aaacagttct ttagtgaagt
    59041 tggtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg ccattagtgc
    59101 ctgccacagt tccaattttc atcttttacc tcatattatt caccgcgagg aatacatagt
    59161 taacatttta tcttatgaca tacacctcat attcgatggg aaaataatta tattttgcac
    59221 aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga gaaagtccct
    59281 aaagattgta gaggcgtcat cagtacatac ctctacaatc gattaagaaa acctatgaaa
    59341 tactggcaaa attaaaaaga gataccacag cactcatgaa gttttattga ggttgaaaac
    59401 aactaagccc tgggacacct tttttatcac cacgttcgtt aggtgctatc ttccaagaat
    59461 ttagccccat ggtataatta tcaatttttt gatgctggtc tacccctaat ccataatggc
    59521 caa
//
LOCUS       DADLZM010000010        53212 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLZM010000010 DADLZM010000000
VERSION     DADLZM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.010"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(12..323)
                     /locus_tag="JNY73_000614"
     CDS             complement(12..323)
                     /locus_tag="JNY73_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY8046732.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="JNY73_000615"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JNY73_000615"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="JNY73_000616"
     CDS             702..1055
                     /locus_tag="JNY73_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY8046733.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="JNY73_000617"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="JNY73_000617"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY8046734.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FINVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="JNY73_000618"
     CDS             complement(2811..3281)
                     /locus_tag="JNY73_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046735.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="JNY73_000619"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="JNY73_000619"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY8046736.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="JNY73_000620"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="JNY73_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY8046737.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="JNY73_000621"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="JNY73_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY8046738.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="JNY73_000622"
     CDS             complement(5284..8433)
                     /locus_tag="JNY73_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY8046739.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="JNY73_000623"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="JNY73_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY8046740.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="JNY73_000624"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="JNY73_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY8046741.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="JNY73_000625"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="JNY73_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY8046742.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISGLLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="JNY73_000626"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="JNY73_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY8046743.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="JNY73_000627"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="JNY73_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY8046744.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="JNY73_000628"
     CDS             14912..15289
                     /locus_tag="JNY73_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY8046745.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="JNY73_000629"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="JNY73_000629"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY8046746.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="JNY73_000630"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="JNY73_000630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY8046747.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWTAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18100..18429
                     /locus_tag="JNY73_000631"
     CDS             18100..18429
                     /locus_tag="JNY73_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY8046748.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="JNY73_000632"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="JNY73_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY8046749.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="JNY73_000633"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="JNY73_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY8046750.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="JNY73_000634"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="JNY73_000634"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY8046751.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="JNY73_000635"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="JNY73_000635"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY8046752.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23894)
                     /gene="aes"
                     /locus_tag="JNY73_000636"
                     /pseudo
     CDS             complement(22934..23894)
                     /gene="aes"
                     /locus_tag="JNY73_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24046..25350
                     /locus_tag="JNY73_000637"
     CDS             24046..25350
                     /locus_tag="JNY73_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY8046753.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25483..27159)
                     /locus_tag="JNY73_000638"
     CDS             complement(25483..27159)
                     /locus_tag="JNY73_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY8046754.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27397..28617)
                     /locus_tag="JNY73_000639"
     CDS             complement(27397..28617)
                     /locus_tag="JNY73_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8046755.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALPGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28835..30487
                     /locus_tag="JNY73_000640"
     CDS             28835..30487
                     /locus_tag="JNY73_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY8046756.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="JNY73_000641"
     CDS             complement(30524..31003)
                     /gene="ybaK"
                     /locus_tag="JNY73_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY8046757.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31207..32001)
                     /locus_tag="JNY73_000642"
     CDS             complement(31207..32001)
                     /locus_tag="JNY73_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046758.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALLVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32139..32480
                     /locus_tag="JNY73_000643"
     CDS             32139..32480
                     /locus_tag="JNY73_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY8046759.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32695..35199)
                     /gene="copA"
                     /locus_tag="JNY73_000644"
     CDS             complement(32695..35199)
                     /gene="copA"
                     /locus_tag="JNY73_000644"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY8046760.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAAFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35461..36393
                     /gene="glsA"
                     /locus_tag="JNY73_000645"
     CDS             35461..36393
                     /gene="glsA"
                     /locus_tag="JNY73_000645"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY8046761.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVLGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36396..37688
                     /locus_tag="JNY73_000646"
     CDS             36396..37688
                     /locus_tag="JNY73_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8046762.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37813..38220
                     /locus_tag="JNY73_000647"
     CDS             37813..38220
                     /locus_tag="JNY73_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY8046763.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38221..38679)
                     /locus_tag="JNY73_000648"
     CDS             complement(38221..38679)
                     /locus_tag="JNY73_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY8046764.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38676..39593)
                     /locus_tag="JNY73_000649"
     CDS             complement(38676..39593)
                     /locus_tag="JNY73_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY8046765.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39739..40416
                     /gene="fetA"
                     /locus_tag="JNY73_000650"
     CDS             39739..40416
                     /gene="fetA"
                     /locus_tag="JNY73_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY8046766.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EVRYELA"
     gene            40403..41182
                     /gene="fetB"
                     /locus_tag="JNY73_000651"
     CDS             40403..41182
                     /gene="fetB"
                     /locus_tag="JNY73_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY8046767.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="JNY73_000652"
     CDS             complement(41245..42099)
                     /gene="cnoX"
                     /locus_tag="JNY73_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY8046768.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="JNY73_000653"
     CDS             complement(42160..42969)
                     /gene="ybbO"
                     /locus_tag="JNY73_000653"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY8046769.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42959..43581)
                     /gene="tesA"
                     /locus_tag="JNY73_000654"
                     /pseudo
     CDS             complement(42959..43581)
                     /gene="tesA"
                     /locus_tag="JNY73_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43552..44238
                     /locus_tag="JNY73_000655"
     CDS             43552..44238
                     /locus_tag="JNY73_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8046770.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44235..46649
                     /locus_tag="JNY73_000656"
     CDS             44235..46649
                     /locus_tag="JNY73_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8046771.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="JNY73_000657"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="JNY73_000657"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY8046772.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="JNY73_000658"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="JNY73_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY8046773.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="JNY73_000659"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="JNY73_000659"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY8046774.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="JNY73_000660"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="JNY73_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY8046775.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="JNY73_000661"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="JNY73_000661"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY8046776.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53212
                     /locus_tag="JNY73_000662"
     CDS             52939..>53212
                     /locus_tag="JNY73_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY8046777.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12818 a        13706 c        14023 g        12665 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aacgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ctggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccgg gaagggcagg atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttt atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 taataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctgg ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgcgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaatcagcga gaatttgctg gcgtaatgtg tgctcttccg ctagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc tccgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgca ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccagccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctagactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaacc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgtttttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgctacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cggactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gcggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 cctgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccctggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtggacggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggcgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaac
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtattg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagcc ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct catccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag cgccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactcctcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatc aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgccttc catgcaaata aaatagcgtc
    23641 gccgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga
    23701 accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc
    23761 gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccaggacgg taaatccggc
    23821 tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac aggtagtttg
    23881 ttttccggct tcatacaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat
    23941 gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga
    24001 ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt
    24061 aaacgtaaat ccaaaaatta cttccctgta aatgcacgcg atccgctgct tcagcaattc
    24121 cagccagaaa acgaaaccag cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat
    24181 attgaagcga aagtggatga tgaattcatt gagcgttatg gattaagcgc cgggcattca
    24241 ctggtgattg aggatgacgt agccgaagtg ctttatcagg agctaaaaca gaaaaacctg
    24301 attacccatc agtttgcggg tggcaccatt ggtaacacca tgcacaacta ctcggtgctc
    24361 gcggacgacc gttcggtgct gctgggcgtc atgtgcagca atattgaaat tggcagttat
    24421 gcctatcgtt acctgtgtaa cacttccagc cgtaccgatc ttaactatct acaaggcgtg
    24481 gatggtccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct
    24541 atcagtccag gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc
    24601 ggagcctcgg cactggttct cacctcatat ctggtgcgtt gcaagccggg tgaacccatg
    24661 ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg
    24721 acgctgggca ccaagtttgt cattgccgag aatccgcagt ggtggcagca attcctcaaa
    24781 gaccacgttt ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc
    24841 gatccgttgt tggcatctga caaggcgctg gactgggtag atctggtgct gtgcaccgcc
    24901 gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag
    24961 catccgctgc tgccgggcgc tatagcggaa ttcaaccagt atgagtttag ccgcgccatg
    25021 cgccacaagg attgccagaa tccgctgcgt gtctattcgc acattgcgcc gtacatgggc
    25081 gggccggaaa aaatcatgaa cactaatgga gcgggggatg gcgcattggc agcgttgctg
    25141 catgacatta ccgccaacag ctaccatcgt agcaacgtac caaactccag caaacataaa
    25201 ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc
    25261 tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac
    25321 agcctggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc
    25381 gttcacgccg catctggcat ttggctctcg atgcctgatg cgacgctggc gcgtcttatc
    25441 atgcctacat atttttcata ttttacatcc ggcaaccacc gtttaccccg tcaccacttc
    25501 acccgccggt ggcgtttcca gcagttccag catagtacgg gcaatttcac gttcacccat
    25561 cactacctga ttcgcgccac gttcggtgat atacgtcact tcatcgtcat aatgggcgcg
    25621 ggcgataatc tcaatatccg ggtttttcgc gcgggcagac gccacaatct cacccgcttc
    25681 ataaccgttg gggatcgtca ggatcagcca ttttgcacat tccagatgtg ccagttgcat
    25741 aatttcttcg ttcgctgcat tgcccaatac agcgcggacc ccacgctctc gcagctcatc
    25801 aacacgggtt cgtgacgtct caatcaccac cagcggaata tcagaggcga gcaatttctc
    25861 ccccagcagg ctgcctacac gaccgtaacc caccaatagc gcatggttgc aaatatccac
    25921 tgggatctgt ttctcttctt cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt
    25981 cttcgccaga tatttctcca gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat
    26041 cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt aaattcaatg ccattcccag
    26101 tcccgccagg ataaacgcga actcaccaat ctgcgccagg ctggcggcga tggttaatgc
    26161 cgtacgttgg gagtgaccaa acagacggac aaggaacagg gccgcgaccg atttaccaaa
    26221 cagaataatt gccagcgtcg ccagcactgc cagcggttgc tgaatcagaa ttaacggatc
    26281 aaacaacatc ccgactgaga caaaaaacag caccgcaaac gcgtcgcgca atggcaacgt
    26341 atcgtgggcg gcacggtgac tcaattcaga ctcgttcagt accatcccgg caaagaacgc
    26401 accgagtgca aaggagacat caaacagctc taccgcacca aaggcaatcc ctaacgccag
    26461 cgccagcact gacagggtaa acagctcgcg agaaccggtt gccgcgctgc gtgccataat
    26521 ccacggcacc agacggcgac ctaccagcat cataatggcg ataaatgcga tcaccttgcc
    26581 gatggtgatc cccatatcga ctgcaagagt ggcaaagccc acatcgccct gttccatcat
    26641 tcctgccact gcgggcagca acaccagcgt cagaaccatc accaggtctt ccacaatcaa
    26701 ccaaccgatg gcgatttgcc cacgctgact gtctattaat tgccgttctt caagtgcgcg
    26761 cagtaacacc acggtactgg cggtggaaag acataagccg aacacgatac cggtcattaa
    26821 cgaccagccc agcacggcgg agagcgccat acccagcagc gtcgccacgg ctatctgggc
    26881 gatcgcgccg ggaatggcga tggactttac cgccatcaaa tccttgagcg aaaaatgcaa
    26941 accgacgccg aacatcagca aaataacgcc cagttcagcc agttccggcg caagcttggt
    27001 atcggcaaca aagcccggag tgaatggtcc tgccagcaca cccgctaaca gatatcccac
    27061 cagaggagaa atacgtagtt tattggccag catgccgagg ataaaggcga gcacaaggcc
    27121 gccaacaatg gtggtgataa gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg
    27181 ttattgtcca tttttggccg ggaaaaccaa aattacaggt aatagtttat gacaatttca
    27241 ttgatgatgt ttatgaataa ttgttgaatt ttgcagaaaa atggaattag ctgcaaaaaa
    27301 agcacggatc ggaaaacaga aggcggttta acagaggaaa ggcttatggc gactgtaaaa
    27361 cgatgcagcc aaagtttgcc tttggctgca atgaaatcag tctttatgcc ggttatcagg
    27421 caggaatatg gtcaacatcc ccaatagtgg caggaaagca cagattttat agactaactc
    27481 gatgctggtg tgatcggcga taagccccag aactgccgct cccagacctc ccatgccgaa
    27541 agcaaaaccg aaaaagagtc cagaaatcat accgatacgt cccggaagta gctcctgagc
    27601 gtagaccaga atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac
    27661 ccccgtccag tgcaggcggg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat
    27721 agagccccaa atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt
    27781 acctgccgca acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa
    27841 tccgaatttt tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata
    27901 tttcgagaaa atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag
    27961 tggattgatt atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca
    28021 acggctaatt tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac
    28081 gttgcctttg ccataaggcg cgataatcac cgccgccagc aaggggccca gggaactgcc
    28141 aaagttgccg ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat
    28201 acgggctacg cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc
    28261 cgccagcaga actgcgccaa aactgcccgg cagcgcaagc agcaccagac cacttaaggt
    28321 aaagcacatg ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac
    28381 cactggttgc agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat
    28441 aaatgtcaga gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat
    28501 catgtcgttc agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc
    28561 cttggtcgtt gacgcagccg cgcccgccac aggctgggtt tgttcactca ttgccatagg
    28621 aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac
    28681 taacatacct gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc
    28741 tggatgcaga tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt
    28801 tttaacacgt tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc
    28861 gttagcgctg ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga
    28921 taaaacctac aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa
    28981 tgaatatggc gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga
    29041 ggttgcggct gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt
    29101 gcccgagtct gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta
    29161 tgacgcgatg gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca
    29221 ggaaaagtgg gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga
    29281 gcgcctgttt aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg
    29341 gctgacaacc gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt
    29401 tcgtaagccc gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc
    29461 agacattatt atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa
    29521 cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt
    29581 cggtggtcac tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta
    29641 cgtgccgggt acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca
    29701 tgagtggggc aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat
    29761 ggttaactac cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa
    29821 aagcgagcgc gtgctttaca ctcctgaaat cgctgaaaac cagcaaatga tctcgctgtt
    29881 atcaccgttc cagaacaaag gcaaagcgca gctggaagtg aaaataggca aaaccaatgg
    29941 tcgtctggaa ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat
    30001 tctggcagcc caaatggatc gcactggtgc cgactttgcg gtgatgagcg gaggcggaat
    30061 tcgtgattct atcgaagcag gcgatatcag ctataaaaac gtgctgaaag tgcagccatt
    30121 cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag gtgattgatt acctgaccgc
    30181 cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt
    30241 ggcgaaagac ggcaaactga acgaccttaa aatcaaaggc gaaccggtcg atccggcgaa
    30301 aacttaccgt atggcgacat taaacttcaa tgccaccggc ggtgatggat atccgcgcct
    30361 tgataacaaa ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta
    30421 tatccagaaa agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg
    30481 gcagtaatcc gaaagtgccg gatgtttgca tccggcacaa tgcttaatcg cggcgggcga
    30541 tatcagcaaa tttggcatcg aggatctttg ccagatcgcc tgccgccagt tcgatatcca
    30601 gtccgcgctt gccgccggaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa
    30661 tcgttggcag acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg
    30721 aacgctgcgc gaccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta
    30781 cttttttaag atccagttga cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat
    30841 caccgttcac tgccaccagc agcgttttgt agacctgatc cggattcaaa cctaattttt
    30901 tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa
    30961 acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt
    31021 aaacaaaaaa acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc
    31081 ctaagagtat tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag
    31141 tgacggcata ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc
    31201 atttttttag cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac
    31261 cgcgaccaca tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg
    31321 ctgatgcatc agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt
    31381 attttgcggc ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt
    31441 atgccagtgg gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa
    31501 catggcaatc tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc
    31561 cgcctgcaat agctggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg
    31621 cgtcgcctga agaaccatcg cgatttgcca cagcggttgg gtagaaaaga gtgagggaga
    31681 aatgcccatc tcctggctaa tgtgctgcag gttttggagt tgctcctcgc taatgcgctc
    31741 ttccagcgcc tcgcaggcag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc
    31801 ctcgacgagc agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc
    31861 catatcgtgg ctacccatat gaatactgcc aatcagatga aaatggcgat tgccggggag
    31921 ggtgatatcg atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt
    31981 tacccggtac aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg
    32041 agggatggcg caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc
    32101 ttgcatcgct attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac
    32161 cgactacacc cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca
    32221 atgagttagc agagttgctg catgttcatc gtaatcgcgt cagtgcactg atcaataaca
    32281 atcgtaaact caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg
    32341 attttaggct aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca
    32401 cccaggaaga attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc
    32461 gtgcaaaaaa ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt
    32521 tcacgccgca tccggcatcc gagcaccatt gcctgatgcg acgcttacgc gtcttatcag
    32581 gcctacaaac ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt
    32641 catgcgtcga tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc
    32701 cttcggttta aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc
    32761 cattgccgct ccggcaacta ccgggttaag cagtgttcca gtaaacggcc acaaaatacc
    32821 ggcggcgacc ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat
    32881 gttgcgcagc gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg
    32941 catcagggta atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc
    33001 gacatccgcc tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg
    33061 acgtccttca ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc
    33121 aatcacttca tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc
    33181 ggtcaacatc accagacgat atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc
    33241 actacgcaac ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag
    33301 cacaggcgtt gctccttgcg atgcctgagc tgaaatatcg gcttcaatcg ctttagtatc
    33361 gacttgctga tcatttaaca acgcctgatt acccagcaat aacgcatgac cttctgcttc
    33421 accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg acctgcggta gctgcatatc
    33481 gcctgcttta tcaaggatcg ctcgtgccaa tggatggctg gaaccttgct ccagcgccgc
    33541 cgccagacgc agcgcctgcg cttcgtcaac atcagcaaat gttttcactg cgacaacctg
    33601 cggcttccct tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc
    33661 gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc
    33721 ggaaataatc gacatcggcg tcgccagtcc cagcgcacac gggcaggcga taatcagcac
    33781 cgtagtggca atcaccaggg tatagacaat ctgcggtgcc ggaccaaaga aataccagat
    33841 tgccgcactg acaagcgcaa taaccactac taccggcaca aatgcggctg agattttatc
    33901 cgccagctga ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatcct
    33961 tgatagcgtg gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac
    34021 cactgtcccg gcatggacgc tatcgccttc gcctttttgc tgcgggattg gttcgcccgt
    34081 cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg
    34141 atcgccggtc gtcaggcgca gcaacatacc tggctgcact tctgccagag gcacgctttt
    34201 ttcaccttcg tcagtaacca ggcgcgccgt cggtggggtt aaatcgagta atttctccag
    34261 tgccttagaa gaacgctgac gtgcgcgtgc ttccagcata tggccgagat tgatcagacc
    34321 gataatcatc gcactggctt catagtaaag atgtcgcgct tccatcggga accactgcgg
    34381 ccacaggttg acgctcatcg aatagagcca cgccacgccc gttcccagcg ccaccagcgt
    34441 atccatcgtc gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc
    34501 ggcgaaaacc atcaccgcca gggttatcag gccgataacc aaccacaggc tgcggttgtc
    34561 ggcggtgacc atcatgttat cgccgatcat cccccagacc atcaccggga tacccaccgc
    34621 cagtgcgaca attgcctgcc agcggaagcg cttcatcgta gcgacggcgg tttcttgctg
    34681 gcgttcgcgg cgtttcgcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac
    34741 cgcctgcact aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag
    34801 gtttacccgt gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac
    34861 acagctggcg cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt
    34921 cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc
    34981 cagcggttta gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc
    35041 gatcagctgt tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc
    35101 ttgttccaca tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca
    35161 ggacaggccg tccaggttca ggtcgatagt ttgtgacata aaacactcct ttaagacagt
    35221 tttgactggc tgtgataaag gttaaacctt ccagaaaggg gaaggtcaag aaattaataa
    35281 accaggcggg taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga
    35341 catttatttc tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct
    35401 gaaaatcggc acaggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag
    35461 atgttagatg caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca
    35521 cttaacggcg gacaaaatgc cgattacatt ccctttctgg cgaatgtact aggtcaactg
    35581 gcggcagtgg ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac
    35641 cgctttgcac tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc
    35701 ggcccgcagg cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca
    35761 gttatcgcct tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgct
    35821 attgccacca ccagcctgat taacgctgaa aacgccgaac aacgctggca gcgaatttta
    35881 catatccaac agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg
    35941 gaacaaacaa ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat
    36001 ctctattgtg atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc
    36061 aatactgttg aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg
    36121 acgcataaac gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg
    36181 gaagggctgt atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc
    36241 ggtgtaggtg gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca
    36301 ccaccgctgg acgaagaagg caatagtgtt cgcggtcaaa aaatggtggc atcggtcgct
    36361 aagcaactcg gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg
    36421 gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg
    36481 cggggatctt cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctggggcg
    36541 cctttgcttt tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg
    36601 cgagctatcc cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg
    36661 tcttttcgct agcgctctcg ttgctatacc tgttgacgct ggcggtgagc atcgccatgg
    36721 ttgcccgtgc ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc
    36781 accttatttt gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa
    36841 gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt
    36901 tattgctgat tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc
    36961 cccccagctc cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct
    37021 ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg
    37081 cgtttctggt ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc
    37141 ttagcgatgt atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg
    37201 cttctccgct gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga
    37261 cggcttcagg cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca
    37321 gcgaacgcga actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca
    37381 acatcatcgt cgtggtgttg ataatgctga tgacggcggc gctaaattta gactcactcg
    37441 ccagcgttgc cagcgccacc tttttgatct gctatctggc ggtgtttgtg gtggcgatcc
    37501 gcctgcgtca tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt
    37561 tgttggtgat cgttggcttt atctacagcc tgtggtccca gggcagccgt gcgttgatat
    37621 ggattattgg tgcaatctta ctcagcctta ttgtggcaat ggtcatgaag cgtaataaaa
    37681 ccgtataaca cctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc
    37741 gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc
    37801 gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc
    37861 attcgcttct atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat
    37921 cgcacctaca cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg
    37981 ggctttaacc tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac
    38041 agcgccgacg tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag
    38101 gagctgcaat ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac
    38161 agcgccgact gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga
    38221 ttaagacgag acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacata
    38281 cgtacctgca ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat
    38341 atgaccgcgc ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg
    38401 cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca
    38461 tttccaccac agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc
    38521 ccaacccagc ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca
    38581 caacaaataa ccatttccgc ccagcatctc ggctgccagc agcaacccgc cgagactgag
    38641 ccagaaaata tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg
    38701 ctgtctttca ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat
    38761 ggcatcatca ctactttgct gttactggag gaaccgatct gctgtaacgc ttcggtgtat
    38821 ttttgcgcta cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc
    38881 attttggtgg cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac
    38941 gccgactgac gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg
    39001 aggatttccg cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc
    39061 gctttcatct gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt
    39121 tcaatacggg tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg
    39181 cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg
    39241 gtacggatgt tagtcatggt caggttgatg atcgccagct ccagattgct gacttcatag
    39301 gcagcgcgtg gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg
    39361 ttatctttcg agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc
    39421 ttgcgaccaa tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg
    39481 gtatagcgac caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc
    39541 gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa
    39601 acctcctgtt gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct
    39661 gattactgaa agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat
    39721 cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga
    39781 tatctggcgg gtgatacgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa
    39841 tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct
    39901 tcattgatca gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta
    39961 aaaccagaaa tctaccgcca gcaagtctct tactgcgccc agacgccgac gctgtttggc
    40021 gatacggtat acgataatct gatctttccc tggcagatcc gtgaccagca gcctgacccg
    40081 gccatttttc tcgattttct cgaacgcttc gctttgccgg acagcatttt gacgaagaat
    40141 atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat tgattcgtaa cctgcaattt
    40201 atgccgaagg ttttattgct ggatgaaata accagtgcgc tggatgaaag taataaacat
    40261 aacgtcaatg agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg
    40321 acacacgata aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc
    40381 ggagaaatgc aggaagtacg ctatgaactc gcataatatt actaacgaat cattagcact
    40441 ggcattaatg ctggtggtgg tcgcaatctt aattagccat aaagaaaaac tggcgctgga
    40501 gaaagatatt ctctggagcg tcgggcgagc gataattcag ctgattattg tcggctatgt
    40561 gctgaagtat attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat
    40621 ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat
    40681 ctcatcgttt attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc
    40741 agggtcgatt gaatttatcc cgatgcaggt gatccctgtc gccgggatga ttgccggtaa
    40801 cgccatggta gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca
    40861 gcaacagatt caggagaaac tgagtcttgg cgcgacgccg aagcaggctt cagcgatatt
    40921 gattcgcgac agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg
    40981 cttagtgagt ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa
    41041 ggcgattaaa tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac
    41101 cattattgcc tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt
    41161 gactcaattg aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg
    41221 gttcatgtgc cgaacaacgg aatatcaata caacaatgca tacagctggc ggcgatactt
    41281 cgacgccagt gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt
    41341 ctgaccgtct gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc
    41401 ttcattgcgc ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc
    41461 tggattctcc gccacctgct gttgcaactg ttgaatttcc ggcttatcag ccgcctgctt
    41521 cagcagttcg atttgcgcta ccaggccctg gtagcgggtg tcctgatcct gcaacggaat
    41581 ggttttcagc accgcttccg catcttcaga acggttcagc gcaatcagcg tttctgccag
    41641 cagcaggccg atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag
    41701 ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc
    41761 ttcgcgcggc agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg
    41821 gaagccatct accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc
    41881 aaactgggcg gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc
    41941 gttgtactgc gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg
    42001 ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag
    42061 gttagattcg ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga
    42121 tattttcttt gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc
    42181 catcacgcgc ccaggcagca ggcgctcgag caccattact gcccaggtca ccagcgttac
    42241 cggatagcgc atcttcggct tctcgctaat aaaagcatgg cgcactttgt cgaccaccgc
    42301 ttccggtccc aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg
    42361 cgtctggttg acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac
    42421 tttaattccg ctgtggcgta gctccatgcg cagcgcgtct gaccacgcct ccagcgcata
    42481 tttactggcc gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt
    42541 catcacaata cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg
    42601 gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga
    42661 aagggggcca tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt
    42721 cagggcgatc acctcgtctg ctgcgcgatt aacactttct ggtgaatcca gatcgatcaa
    42781 cacgccggta aatcccatgt tgttcatacg ctcaacatca ttcggttttc ggcaacctgc
    42841 cagcacatga aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga
    42901 acatccggta attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt
    42961 atgagtcatg atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct
    43021 gggcgtcgcg gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct
    43081 cttccataaa aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg
    43141 cgctaaaggc ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca
    43201 atggttcagc gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt
    43261 gctgtggctg aaaaccacgc aaaccatcat tgcgcccagt tcaaccagca cccaacgcgg
    43321 ctgatgctgt ttgagcagag ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct
    43381 gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
    43441 ggccgcgctg gcagacattc gatatccggc gctcaggcta tcacccagaa tcaataacgt
    43501 gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg
    43561 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
    43621 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
    43681 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc
    43741 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
    43801 aagttgcgcg cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt
    43861 aacgcgctgg aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt
    43921 cgtaacgggg cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt
    43981 ccggcacagc tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt
    44041 cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat
    44101 aaaattgccg acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg
    44161 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
    44221 ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat
    44281 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
    44341 gcgatcgcat ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg
    44401 cgttgcgcag ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc
    44461 tgaaagtcgg caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc
    44521 tggcgaacgt caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc
    44581 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc
    44641 ttaacctgaa aacggacgac accattgacg tgggcgatgc caccttgcgg attgccggag
    44701 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
    44761 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
    44821 gttataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg ttgttacctc
    44881 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
    44941 cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag
    45001 tggcagcggt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
    45061 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
    45121 tgatggtgct gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg
    45181 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
    45241 cgtggctgtg ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat
    45301 accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg gtcgcgaacg
    45361 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
    45421 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
    45481 tgctgtgcgg tgtgctgggc tggatgctgc tgaatgtact tcgccgcatg acgctgaaat
    45541 cgctgcctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc
    45601 agctttcggc attttcgctc tcctttatgc tgctggcact gctgctggtg ctgcgtggcg
    45661 atctgctcga ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta
    45721 acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    45781 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag
    45841 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    45901 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    45961 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    46021 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    46081 ggcctaattt ctattttatt ttccctgaag gggcattaga cgggcaaccg cagagctggc
    46141 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    46201 cgaccattag cctgttagac attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    46261 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    46321 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    46381 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgagttc gccatgcttg
    46441 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    46501 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    46561 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    46621 aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg atgcgctacg
    46681 cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat
    46741 aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca
    46801 actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt agctacgttt
    46861 cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg ccttaagaac
    46921 gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag aaagacaatc
    46981 acagcttacc cttacgttgc ctgtaactat gattttcttc atgtgctaga tgaggactgc
    47041 ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag gacttaaaag
    47101 tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc gggattgtat
    47161 cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt gctagatgag
    47221 gactgtttaa tttctgcccg gaacaaccaa gtcttgggcg gaaaaaataa aaaaaccgac
    47281 tgtcatgtcg gttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gctagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggcgg cgcgttcatc gttattcatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtgggttgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgccgctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 gggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattgatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggtaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agcgttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttaaaagttg gaaaattttt
    51121 ccaattaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgatgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct gggggtttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gttggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat tc
//
LOCUS       DADLZM010000011        49573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLZM010000011 DADLZM010000000
VERSION     DADLZM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.011"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1224
                     /locus_tag="JNY73_000663"
     CDS             <1..1224
                     /locus_tag="JNY73_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8046778.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="JNY73_000664"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="JNY73_000664"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY8046779.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="JNY73_000665"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="JNY73_000665"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY8046780.1"
                     /translation="MSTLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="JNY73_000666"
     CDS             2714..3427
                     /locus_tag="JNY73_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY8046781.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="JNY73_000667"
     CDS             complement(3399..4076)
                     /locus_tag="JNY73_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY8046782.1"
                     /translation="MLTLNKISYRWPGAATDCLCGISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="JNY73_000668"
     CDS             complement(4070..4747)
                     /locus_tag="JNY73_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY8046783.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="JNY73_000669"
     CDS             complement(4735..5118)
                     /locus_tag="JNY73_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY8046784.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="JNY73_000670"
     CDS             complement(5134..5442)
                     /locus_tag="JNY73_000670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046785.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="JNY73_000671"
     CDS             complement(5443..6021)
                     /locus_tag="JNY73_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY8046786.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="JNY73_000672"
     CDS             complement(6044..6475)
                     /locus_tag="JNY73_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8046787.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="JNY73_000673"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="JNY73_000673"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY8046788.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="JNY73_000674"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="JNY73_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY8046789.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="JNY73_000675"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="JNY73_000675"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY8046790.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /locus_tag="JNY73_000676"
     CDS             10564..11424
                     /locus_tag="JNY73_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY8046791.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="JNY73_000677"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="JNY73_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY8046792.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="JNY73_000678"
     CDS             12291..13031
                     /locus_tag="JNY73_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY8046793.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..13707
                     /locus_tag="JNY73_000679"
     CDS             13198..13707
                     /locus_tag="JNY73_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8046794.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPL"
     gene            13831..14093
                     /locus_tag="JNY73_000680"
                     /pseudo
     CDS             13831..14093
                     /locus_tag="JNY73_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451535.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNY73_000681"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNY73_000681"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY8046795.1"
                     /translation="METQWTRMTADEAAEIILHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNY73_000682"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNY73_000682"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY8046796.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNY73_000683"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNY73_000683"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY8046797.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNY73_000684"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNY73_000684"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY8046798.1"
                     /translation="MPNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /locus_tag="JNY73_000685"
     CDS             complement(19724..20617)
                     /locus_tag="JNY73_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY8046799.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="JNY73_000686"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="JNY73_000686"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY8046800.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /locus_tag="JNY73_000687"
     CDS             21915..23147
                     /locus_tag="JNY73_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY8046801.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="JNY73_000688"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="JNY73_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY8046802.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="JNY73_000689"
     CDS             23768..23827
                     /locus_tag="JNY73_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY8046803.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23924..24526)
                     /locus_tag="JNY73_000690"
     CDS             complement(23924..24526)
                     /locus_tag="JNY73_000690"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY8046804.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24547..24730)
                     /gene="ssrS"
                     /locus_tag="JNY73_000691"
     ncRNA           complement(24547..24730)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JNY73_000691"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="JNY73_000692"
     CDS             complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="JNY73_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY8046805.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25269..25847
                     /gene="ygfB"
                     /locus_tag="JNY73_000693"
     CDS             25269..25847
                     /gene="ygfB"
                     /locus_tag="JNY73_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY8046806.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25873..27198
                     /gene="pepP"
                     /locus_tag="JNY73_000694"
     CDS             25873..27198
                     /gene="pepP"
                     /locus_tag="JNY73_000694"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY8046807.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHARGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27195..28373
                     /gene="ubiH"
                     /locus_tag="JNY73_000695"
                     /gene_synonym="visB"
     CDS             27195..28373
                     /gene="ubiH"
                     /locus_tag="JNY73_000695"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY8046808.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28396..29598
                     /gene="ubiI"
                     /locus_tag="JNY73_000696"
                     /gene_synonym="visC"
     CDS             28396..29598
                     /gene="ubiI"
                     /locus_tag="JNY73_000696"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY8046809.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(29672..29755)
                     /locus_tag="JNY73_000697"
     CDS             complement(29672..29755)
                     /locus_tag="JNY73_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046810.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30046..31140
                     /gene="gcvT"
                     /locus_tag="JNY73_000698"
     CDS             30046..31140
                     /gene="gcvT"
                     /locus_tag="JNY73_000698"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY8046811.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31164..31553
                     /gene="gcvH"
                     /locus_tag="JNY73_000699"
     CDS             31164..31553
                     /gene="gcvH"
                     /locus_tag="JNY73_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY8046812.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31672..34545
                     /gene="gcvP"
                     /locus_tag="JNY73_000700"
     CDS             31672..34545
                     /gene="gcvP"
                     /locus_tag="JNY73_000700"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY8046813.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34812..35555
                     /locus_tag="JNY73_000701"
     CDS             34812..35555
                     /locus_tag="JNY73_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8046814.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVVQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35612..37045)
                     /locus_tag="JNY73_000702"
     CDS             complement(35612..37045)
                     /locus_tag="JNY73_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8046815.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37090..37401
                     /locus_tag="JNY73_000703"
     CDS             37090..37401
                     /locus_tag="JNY73_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY8046816.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            37565..38224
                     /locus_tag="JNY73_000704"
     CDS             37565..38224
                     /locus_tag="JNY73_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY8046817.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="JNY73_000705"
     CDS             complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="JNY73_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY8046818.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39643..39909
                     /gene="sdhE"
                     /locus_tag="JNY73_000706"
     CDS             39643..39909
                     /gene="sdhE"
                     /locus_tag="JNY73_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY8046819.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39890..40297
                     /locus_tag="JNY73_000707"
     CDS             39890..40297
                     /locus_tag="JNY73_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046820.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="JNY73_000708"
     CDS             complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="JNY73_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY8046821.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40970..41866
                     /gene="xerD"
                     /locus_tag="JNY73_000709"
     CDS             40970..41866
                     /gene="xerD"
                     /locus_tag="JNY73_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY8046822.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41891..42601
                     /gene="dsbC"
                     /locus_tag="JNY73_000710"
     CDS             41891..42601
                     /gene="dsbC"
                     /locus_tag="JNY73_000710"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY8046823.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42607..44340
                     /gene="recJ"
                     /locus_tag="JNY73_000711"
     CDS             42607..44340
                     /gene="recJ"
                     /locus_tag="JNY73_000711"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY8046824.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44431..45529
                     /gene="prfB"
                     /locus_tag="JNY73_000712"
     CDS             join(44431..44505,44507..45529)
                     /gene="prfB"
                     /locus_tag="JNY73_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY8046825.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45539..47056
                     /gene="lysS"
                     /locus_tag="JNY73_000713"
     CDS             45539..47056
                     /gene="lysS"
                     /locus_tag="JNY73_000713"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY8046826.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47099..47647)
                     /gene="idi"
                     /locus_tag="JNY73_000714"
     CDS             complement(47099..47647)
                     /gene="idi"
                     /locus_tag="JNY73_000714"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY8046827.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARILLSAFTQLK"
     gene            47702..47773
                     /locus_tag="JNY73_000715"
     CDS             47702..47773
                     /locus_tag="JNY73_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046828.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47770..47895)
                     /locus_tag="JNY73_000716"
     CDS             complement(47770..47895)
                     /locus_tag="JNY73_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046829.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47897..49345)
                     /locus_tag="JNY73_000717"
     CDS             complement(47897..49345)
                     /locus_tag="JNY73_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY8046830.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11967 a        12404 c        13348 g        11854 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg tcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgccgcaa
     4021 agacaatccg ttgccgcgcc tggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatttctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 tgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcatt gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgtgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgcaggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ctaggttata
    15961 cgggacaccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttaggcat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gttaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta
    23881 aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt
    23941 tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca
    24001 ccaactgaca atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta
    24061 aggtccgatc ataaaaacca ccgcccattc ccaggcgctg accgtactca tcaaaggcga
    24121 ccagcggtgt gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt
    24181 ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga
    24241 acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc
    24301 gccagagttg ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag
    24361 cgaccgtatg cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac
    24421 ccatttccag ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga
    24481 tttcttgtcg ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat
    24541 aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt
    24601 gaatgtgtcg tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg
    24661 agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc
    24721 tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa
    24781 gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg
    24841 ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc
    24901 ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt
    24961 gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt
    25021 caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat
    25081 atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt
    25141 agacccatgg tctgcgaagg ggcgacgcta tagctaccct gatgagaaga gacaagccct
    25201 tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac
    25261 gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga
    25321 accaacaagg gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat
    25381 gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca
    25441 tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg
    25501 ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg
    25561 ttttcgatcg ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg
    25621 ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc
    25681 gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc
    25741 ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc
    25801 atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt
    25861 aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg
    25921 gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt
    25981 agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt
    26041 aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt
    26101 ctgtttaacc gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag
    26161 gatgccgcgc cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag
    26221 caactttatc aactacttaa cggcctggat gtggtttacc atgcccgggg cgaatatgca
    26281 tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat
    26341 ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc
    26401 aaatcgccag aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat
    26461 acacgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt
    26521 caccacgaat ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc
    26581 ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac
    26641 ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc
    26701 ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag
    26761 tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt
    26821 gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt
    26881 gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg
    26941 ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa
    27001 ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca
    27061 gaacaatatc gcggtatcgg cattcgtatt gaagacgaca ttgtgattac cgaaaccggc
    27121 aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct
    27181 gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg
    27241 cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg
    27301 cgccagagtc acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta
    27361 cctgtcagca actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca
    27421 tcactaccgt gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag
    27481 attaccaact ggcggcgctg gggcaggttg tcgaattgca taatgttggg caacggctgt
    27541 ttgcattgct gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg
    27601 ttgcccgtac tcagagtcac gttgaagtga cgctggagag cggcgagatg ctgacgggcc
    27661 gcgtgctgat agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt
    27721 ggcagcagga gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc
    27781 acgaaggccg cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt
    27841 ctgacggacg ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt
    27901 cgtggagtga cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc
    27961 aaattaccca cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac
    28021 cgattaccca tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg
    28081 ggcaagggtt taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg
    28141 cgcatgagcg cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc
    28201 gacagagcga tcgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca
    28261 accgttgggc accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca
    28321 ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt
    28381 tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg
    28441 ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc
    28501 gtaccggaac ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc
    28561 gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc
    28621 agttgctatc acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac
    28681 gatcaaagca tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac
    28741 gcgctgtgga acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta
    28801 cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta
    28861 acggcgcgtc tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat
    28921 attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgaccat tcgcacggaa
    28981 gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta
    29041 ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag
    29101 cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat
    29161 cgccttggct tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat
    29221 gcgcgccagt ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac
    29281 ccactggcgg ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa
    29341 ctgaaacggt tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat
    29401 gaacgtagcc gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat
    29461 ctcttttccg gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc
    29521 gacacgcttc ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg
    29581 tctgaatggc tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc
    29641 atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg
    29701 ttttcgcatt atattttcta atgccatgat tttttgattt agtgtttttt gacatttttt
    29761 tagctcttaa tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa
    29821 ttggtgtatt ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta
    29881 atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa
    29941 agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt
    30001 tcaccatgaa aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
    30061 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
    30121 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
    30181 atgtttgatg tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
    30241 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac
    30301 tcggggatgt tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact
    30361 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
    30421 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
    30481 gccgtgcaag ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt
    30541 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc
    30601 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
    30661 gatttctggc gtgcgctggt ggaggcgggc gttaagccct gcggtctggg ggcgcgtgac
    30721 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
    30781 cctttagccg ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc
    30841 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
    30901 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
    30961 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
    31021 gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
    31081 gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
    31141 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
    31201 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
    31261 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
    31321 gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc
    31381 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
    31441 acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agctagcgat gaaagcgaac
    31501 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt
    31561 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
    31621 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
    31681 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
    31741 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
    31801 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
    31861 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
    31921 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
    31981 gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc
    32041 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
    32101 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
    32161 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg
    32221 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
    32281 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
    32341 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
    32401 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
    32461 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
    32521 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
    32581 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
    32641 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
    32701 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
    32761 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
    32821 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
    32881 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
    32941 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat
    33001 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
    33061 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac
    33121 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
    33181 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
    33241 ccaatcactt ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
    33301 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
    33361 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
    33421 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
    33481 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
    33541 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
    33601 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
    33661 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
    33721 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
    33781 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
    33841 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    33901 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
    33961 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
    34021 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    34081 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
    34141 gaagaaatcg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    34201 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
    34261 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
    34321 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
    34381 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    34441 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    34501 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt
    34561 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
    34621 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
    34681 gaatatttac cctcaccccg accatctccc tgaaagggcg agggggaaaa gcgtgccaac
    34741 attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct
    34801 cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggtatc gggcgggcaa
    34861 ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc
    34921 acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc
    34981 aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg
    35041 atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg
    35101 aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc
    35161 tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta
    35221 tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg
    35281 cggcatcgaa aggggcgatt gatacgttaa ccaccggacg atcgctggaa gtcgccgcgc
    35341 aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg
    35401 gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac
    35461 aggcagaaga ggtcgtgcag gccattgtct ggctactaag tgataaagcc tcttacgtca
    35521 cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg
    35581 caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca
    35641 cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca gtaccgtcgt
    35701 catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta gtgaatgaca
    35761 cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg taagtcactg
    35821 cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc
    35881 tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc
    35941 tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc
    36001 agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa ccagagatcg
    36061 catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat
    36121 aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg atattaaatc
    36181 cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc tgcacatcgg
    36241 taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacatg
    36301 agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc
    36361 gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc acctggtaca
    36421 tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag taaccaaaca
    36481 gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag tatttgactt
    36541 tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca acgaatttac
    36601 ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg
    36661 ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg taaaacttca
    36721 gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc caggcgatgg
    36781 aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac
    36841 cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact tctttggtga
    36901 tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc ggtcctttgc
    36961 cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc cataagaaat
    37021 ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat tcttacagga
    37081 aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga tgacattctg
    37141 gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat
    37201 gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga agtcaccgca
    37261 acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga aaatatgacc
    37321 ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca attttatatg
    37381 attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat
    37441 ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac
    37501 tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt ctctggagta
    37561 agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa
    37621 cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt tgctactggt
    37681 tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg
    37741 cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc
    37801 aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta
    37861 cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat
    37921 ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc gattcaaaat
    37981 tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat
    38041 ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc
    38101 gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg
    38161 cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc
    38221 gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca ggcatcggag
    38281 cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg
    38341 ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg
    38401 cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg
    38461 tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
    38521 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac
    38581 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcac
    38641 tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
    38701 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    38761 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa ccagcttcaa
    38821 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    38881 tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    38941 accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc
    39001 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    39061 cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct
    39121 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
    39181 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
    39241 ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
    39301 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    39361 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    39421 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    39481 gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa ttgttgtgaa
    39541 ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    39601 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    39661 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    39721 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    39781 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
    39841 gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    39901 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    39961 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    40021 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta atgctcgcca
    40081 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    40141 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    40201 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    40261 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    40321 taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
    40381 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
    40441 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    40501 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    40561 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaataattt
    40621 taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcttggattt
    40681 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    40741 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    40801 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    40861 cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca
    40921 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    40981 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    41041 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    41101 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    41161 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    41221 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    41281 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    41341 gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    41401 taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    41461 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggggaaga
    41521 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    41581 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    41641 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    41701 tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    41761 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    41821 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    41881 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    41941 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    42001 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    42061 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    42121 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    42181 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    42241 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    42301 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    42361 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    42421 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    42481 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    42541 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    42601 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    42661 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    42721 caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag cggtgtcgaa
    42781 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    42841 gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
    42901 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    42961 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    43021 ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc ggttattgtt
    43081 accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    43141 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    43201 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    43261 attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtagc ggacgtcgtg
    43321 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    43381 aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc acaaaaacta
    43441 gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg gcgactggat
    43501 gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggagaagc gcgcgtgctg
    43561 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaggtt
    43621 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    43681 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgtatcaaa
    43741 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    43801 tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt agacacactc
    43861 taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    43921 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggcc
    43981 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    44041 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    44101 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    44161 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    44221 accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa gctcgatatc
    44281 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    44341 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    44401 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    44461 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    44521 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    44581 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    44641 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    44701 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    44761 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgttacct
    44821 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
    44881 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    44941 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg
    45001 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    45061 cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg atgatgatat
    45121 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    45181 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat
    45241 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg ccatgaagca
    45301 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    45361 ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    45421 ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
    45481 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    45541 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    45601 gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga acgatttccg
    45661 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    45721 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    45781 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    45841 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    45901 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    45961 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    46021 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    46081 taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    46141 tggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    46201 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    46261 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    46321 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    46381 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    46441 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    46501 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    46561 catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    46621 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    46681 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc
    46741 gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg
    46801 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct
    46861 ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta
    46921 cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg
    46981 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    47041 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    47101 atttaagctg ggtaaatgca gataatagta ttctggcttc gcgatttgtc gcctgcatca
    47161 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    47221 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    47281 cggcaaatac cggacacact tcattttcca caatgccact cggatcggtg gcgcggtagc
    47341 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    47401 ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa accgagttag
    47461 tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgcccct
    47521 tggcattaaa cagccaactg gaaaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    47581 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    47641 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    47701 aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt
    47761 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    47821 acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga atatagcgcg
    47881 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    47941 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    48001 catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg gttgcgagca
    48061 taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    48121 agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt
    48181 tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    48241 tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt
    48301 ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat
    48361 gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt
    48421 taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt
    48481 gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    48541 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    48601 taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc ccggataaat
    48661 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    48721 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    48781 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccagcggccc
    48841 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    48901 gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg gtgataaaac
    48961 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    49021 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    49081 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    49141 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    49201 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    49261 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    49321 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag aataaaaagc
    49381 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    49441 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    49501 aatgacaaga gaacctgata atggaattat ctgtaatttt ctcatattga tatttcatta
    49561 atcattctgg cat
//
LOCUS       DADLZM010000012        49251 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLZM010000012 DADLZM010000000
VERSION     DADLZM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.012"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(61..1191)
                     /gene="dnaJ"
                     /locus_tag="JNY73_000718"
     CDS             complement(61..1191)
                     /gene="dnaJ"
                     /locus_tag="JNY73_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY8046831.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1280..3196)
                     /gene="dnaK"
                     /locus_tag="JNY73_000719"
     CDS             complement(1280..3196)
                     /gene="dnaK"
                     /locus_tag="JNY73_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY8046832.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRATD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3573..3977
                     /locus_tag="JNY73_000720"
     CDS             3573..3977
                     /locus_tag="JNY73_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY8046833.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4003..4716
                     /gene="msyB"
                     /locus_tag="JNY73_000721"
     CDS             4003..4716
                     /gene="msyB"
                     /locus_tag="JNY73_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY8046834.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4865..5431
                     /gene="satP"
                     /locus_tag="JNY73_000722"
     CDS             4865..5431
                     /gene="satP"
                     /locus_tag="JNY73_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY8046835.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5466..6053)
                     /gene="mog"
                     /locus_tag="JNY73_000723"
     CDS             complement(5466..6053)
                     /gene="mog"
                     /locus_tag="JNY73_000723"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY8046836.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6168..7121)
                     /gene="tal"
                     /locus_tag="JNY73_000724"
     CDS             complement(6168..7121)
                     /gene="tal"
                     /locus_tag="JNY73_000724"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY8046837.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7400..8854
                     /locus_tag="JNY73_000725"
                     /pseudo
     CDS             7400..8854
                     /locus_tag="JNY73_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            8924..9700
                     /gene="yaaA"
                     /locus_tag="JNY73_000726"
     CDS             8924..9700
                     /gene="yaaA"
                     /locus_tag="JNY73_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY8046838.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(9853..10149)
                     /locus_tag="JNY73_000727"
     CDS             complement(9853..10149)
                     /locus_tag="JNY73_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY8046839.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10363..11649)
                     /gene="thrC"
                     /locus_tag="JNY73_000728"
     CDS             complement(10363..11649)
                     /gene="thrC"
                     /locus_tag="JNY73_000728"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY8046840.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(11650..12582)
                     /gene="thrB"
                     /locus_tag="JNY73_000729"
     CDS             complement(11650..12582)
                     /gene="thrB"
                     /locus_tag="JNY73_000729"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY8046841.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12584..15046)
                     /gene="thrA"
                     /locus_tag="JNY73_000730"
     CDS             complement(12584..15046)
                     /gene="thrA"
                     /locus_tag="JNY73_000730"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY8046842.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15127..15192)
                     /gene="thrL"
                     /locus_tag="JNY73_000731"
     CDS             complement(15127..15192)
                     /gene="thrL"
                     /locus_tag="JNY73_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY8046843.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15406..16092)
                     /locus_tag="JNY73_000732"
     CDS             complement(15406..16092)
                     /locus_tag="JNY73_000732"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY8046844.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16492..16632)
                     /locus_tag="JNY73_000733"
     CDS             complement(16492..16632)
                     /locus_tag="JNY73_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046845.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16728..17444
                     /gene="arcA"
                     /locus_tag="JNY73_000734"
     CDS             16728..17444
                     /gene="arcA"
                     /locus_tag="JNY73_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY8046846.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17503..18855)
                     /gene="creD"
                     /locus_tag="JNY73_000735"
     CDS             complement(17503..18855)
                     /gene="creD"
                     /locus_tag="JNY73_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY8046847.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18913..20337)
                     /gene="creC"
                     /locus_tag="JNY73_000736"
     CDS             complement(18913..20337)
                     /gene="creC"
                     /locus_tag="JNY73_000736"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY8046848.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(20337..21026)
                     /gene="creB"
                     /locus_tag="JNY73_000737"
     CDS             complement(20337..21026)
                     /gene="creB"
                     /locus_tag="JNY73_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY8046849.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGVDDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21039..21512)
                     /gene="creA"
                     /locus_tag="JNY73_000738"
     CDS             complement(21039..21512)
                     /gene="creA"
                     /locus_tag="JNY73_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY8046850.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21723..22592
                     /gene="robA"
                     /locus_tag="JNY73_000739"
     CDS             21723..22592
                     /gene="robA"
                     /locus_tag="JNY73_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY8046851.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22589..23236)
                     /gene="gpmB"
                     /locus_tag="JNY73_000740"
     CDS             complement(22589..23236)
                     /gene="gpmB"
                     /locus_tag="JNY73_000740"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY8046852.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23288..23809
                     /gene="yjjX"
                     /locus_tag="JNY73_000741"
     CDS             23288..23809
                     /gene="yjjX"
                     /locus_tag="JNY73_000741"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY8046853.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(23894..24220)
                     /gene="trpR"
                     /locus_tag="JNY73_000742"
     CDS             complement(23894..24220)
                     /gene="trpR"
                     /locus_tag="JNY73_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY8046854.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24310..26247)
                     /gene="sltY"
                     /locus_tag="JNY73_000743"
     CDS             complement(24310..26247)
                     /gene="sltY"
                     /locus_tag="JNY73_000743"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY8046855.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26458..28125
                     /gene="ettA"
                     /locus_tag="JNY73_000744"
     CDS             26458..28125
                     /gene="ettA"
                     /locus_tag="JNY73_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY8046856.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLDQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGSQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28433..29665)
                     /gene="nadR"
                     /locus_tag="JNY73_000745"
     CDS             complement(28433..29665)
                     /gene="nadR"
                     /locus_tag="JNY73_000745"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY8046857.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(29686..31068)
                     /gene="radA"
                     /locus_tag="JNY73_000746"
     CDS             complement(29686..31068)
                     /gene="radA"
                     /locus_tag="JNY73_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY8046858.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31117..32085)
                     /gene="serB"
                     /locus_tag="JNY73_000747"
     CDS             complement(31117..32085)
                     /gene="serB"
                     /locus_tag="JNY73_000747"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY8046859.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVLANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32191..32835
                     /locus_tag="JNY73_000748"
     CDS             32191..32835
                     /locus_tag="JNY73_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY8046860.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTQLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            32863..33879
                     /gene="lplA"
                     /locus_tag="JNY73_000749"
                     /pseudo
     CDS             32863..33879
                     /gene="lplA"
                     /locus_tag="JNY73_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            33911..34174
                     /locus_tag="JNY73_000750"
     CDS             33911..34174
                     /locus_tag="JNY73_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8046861.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34335..35054)
                     /gene="deoD"
                     /locus_tag="JNY73_000751"
     CDS             complement(34335..35054)
                     /gene="deoD"
                     /locus_tag="JNY73_000751"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY8046862.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35111..36334)
                     /gene="deoB"
                     /locus_tag="JNY73_000752"
     CDS             complement(35111..36334)
                     /gene="deoB"
                     /locus_tag="JNY73_000752"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY8046863.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36386..37708)
                     /gene="deoA"
                     /locus_tag="JNY73_000753"
     CDS             complement(36386..37708)
                     /gene="deoA"
                     /locus_tag="JNY73_000753"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY8046864.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDH
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(37835..38614)
                     /gene="deoC"
                     /locus_tag="JNY73_000754"
     CDS             complement(37835..38614)
                     /gene="deoC"
                     /locus_tag="JNY73_000754"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY8046865.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRYMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            38872..40413
                     /locus_tag="JNY73_000755"
     CDS             38872..40413
                     /locus_tag="JNY73_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY8046866.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSL"
     gene            40385..41248
                     /gene="yjjW"
                     /locus_tag="JNY73_000756"
     CDS             40385..41248
                     /gene="yjjW"
                     /locus_tag="JNY73_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY8046867.1"
                     /translation="MNSRCALVCKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41462..42244)
                     /locus_tag="JNY73_000757"
     CDS             complement(41462..42244)
                     /locus_tag="JNY73_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8046868.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQPGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(42241..43314)
                     /locus_tag="JNY73_000758"
     CDS             complement(42241..43314)
                     /locus_tag="JNY73_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY8046869.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43436..43597)
                     /locus_tag="JNY73_000759"
     CDS             complement(43436..43597)
                     /locus_tag="JNY73_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY8046870.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(43724..44329)
                     /gene="osmY"
                     /locus_tag="JNY73_000760"
     CDS             complement(43724..44329)
                     /gene="osmY"
                     /locus_tag="JNY73_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY8046871.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(44722..46311)
                     /gene="prfC"
                     /locus_tag="JNY73_000761"
     CDS             complement(44722..46311)
                     /gene="prfC"
                     /locus_tag="JNY73_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY8046872.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46402..47079)
                     /gene="yjjG"
                     /locus_tag="JNY73_000762"
     CDS             complement(46402..47079)
                     /gene="yjjG"
                     /locus_tag="JNY73_000762"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY8046873.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47094..47540)
                     /gene="rimI"
                     /locus_tag="JNY73_000763"
     CDS             complement(47094..47540)
                     /gene="rimI"
                     /locus_tag="JNY73_000763"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY8046874.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47509..47922)
                     /gene="holD"
                     /locus_tag="JNY73_000764"
     CDS             complement(47509..47922)
                     /gene="holD"
                     /locus_tag="JNY73_000764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY8046875.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRVNPTARAALWQQICTYEHDFFPRND"
     gene            48025..49056
                     /gene="rsmC"
                     /locus_tag="JNY73_000765"
     CDS             48025..49056
                     /gene="rsmC"
                     /locus_tag="JNY73_000765"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY8046876.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        11552 a        13398 c        12428 g        11873 t
ORIGIN      
        1 catatcttca acgcacccta tttttaccca ggcctgccca cgggcaggct tttggggagg
       61 ttagcgggtc aggtcgtcaa aaaacttctt cacaccatca aagaagctct ttgagcgcgg
      121 gctgttgtgc tcgccggttg ggccaccgaa gctttcttgc agctcttgca gcagctgttt
      181 ctgcttttcg ttcaggccta ccggtgtttc gacgacaacg cggcacagca aatcaccctg
      241 tgcaccaccg cggacagact tgacgccttt accgcgcata cggaatagct taccggtctg
      301 ggtttcgcca ggcactttca gtttgacgcg accatcaagg gtcggtactt cgatttcgcc
      361 acccagcgcc gccatagcga agttgatcgg gacttcgcaa tacaggttgt tgccttcacg
      421 ctcgaaaatc gggtgctgtt taacctgaac ctgaacgtac agatcgcctg ccggtgcgcc
      481 atgctcgccc gcttcacctt cgcccgcaag acggatgcgg tctccagtgt ccacccctgc
      541 cgggatttta acggacagcg ttttgctgcg ctcaacacga ccatgaccat gacatttgtt
      601 gcacggatct ttgatcagcg taccgcggcc ctgacagtgt ggacaggtct gctgtacagc
      661 gaagaatccc tggcgcatct gcacctgacc agaaccatga caggtcggac aggtctgcgg
      721 ctgtgtacct ggttttgcac cgctaccgtg gcaaacgtca cactcttcca gagtcggaat
      781 gcggatttct ttggtcacgc cacgtacagc ttcttcgagg gtgagctcca tgttatagcg
      841 taaatcagca ccgcgcgccg cacgttgacg accacgtccg ccgccaaaaa tatcgccgaa
      901 aacgtcacca aaaatatcgc tgaagtctgc gccgccgcca aaaccaccgc cgcccatgcc
      961 tccttgctca aacgcagcat gaccatactg atcgtatgcc gcacgttttt gcgagtcggt
     1021 cagaacttca taagcttcct tgatctcttt aaatttcgcc tcggcctctt tgtcaccctg
     1081 gttacggtcc gggtggtatt tcatggccag gcgtttgtag gcctttctga tttcacgctc
     1141 ttccgctgtt ttggaaacgc ctaaaatctc gtaataatct tgcttagcca tcttttttaa
     1201 attgccccta gatgaatcca cgggcggaga ggaaattccc cttcgcccgt gtcagtataa
     1261 ttacccgttt atagggcgat tattttttgt ctttgacttc ttcaaattca gcgtcgacaa
     1321 catcgtcatc tttcgcgttg tttgcagaag catcagcacc ggcagtctgc tgctgggcat
     1381 gttgctgctg ggcgatttcc atcagtttct gggaaacctg tgccagttcc tgcattttcg
     1441 cttcgatagc ggctttgtct tcacctttca gagcagtttc cagtgcagtc agcgcagact
     1501 cgatagcagt tttgtcgtca gccggcagtt tgtcgcctgc ttcttcaacc tgcttacggg
     1561 agctgtgcag cagatggtcg ccctggttgc gagtctgtac cagctcttca aacttacggt
     1621 cagcttcggc gttagcttct gcgtcgcgta ccattttctg gatttcatct tcgttcagac
     1681 cagaagaagc cttgatggtg atcttctgct ctttaccgct gtttttatct ttcgcggaaa
     1741 cgtgcaggat accgtcagca tcgatatcga aggtaacttc gatctgcggc atgccgcgcg
     1801 gtgccgggtt gataccatct aggttgaact gacccagaga tttgttatca gtcgcacgtt
     1861 tacgttcacc ctgcagcaca tggatggtta ccgcagactg gttgtcttca gcggtagaga
     1921 acacctggct gtgcttggtc gggatagtgg tgtttttcgc gatcagcgtc gtcatcacac
     1981 cgcccatggt ttcgataccc agagacagcg gggtaacgtc cagcagcagt acgtctttta
     2041 cgtcaccagt cagaacacca ccctgaacag cagcaccgat tgctacagct tcgtccgggt
     2101 taacgtcttt acgcggctct ttaccaaaga actcagcaac tttcttctga accattggca
     2161 tacgagtctg accaccaacg aggataacgt cgtcgatatc agatacggac aggccagcat
     2221 cctgcagtgc aactttcagc ggctcaatgg aacggtttac cagatcttca accaggcttt
     2281 ccagtttcgc acgagtcact ttgatgttca tgtgtttcgg accggtcgcg tctgcagtga
     2341 tgtatggcag gttaacgtcg gtctgctgag cggaagacag ttcgattttc gctttttctg
     2401 ccgcttcttt caggcgctgc attgccagcg gatcgttgcg caggtcaatg ccctgatctt
     2461 tcttgaattc ttcaaccaga tagttgatca gacggctgtc gaagtcttca ccacccaggt
     2521 gggtatcacc gttggttgcc agaacttcga aggttttttc gccgtcaact tcgtcgattt
     2581 cgataataga aatatcgaaa gtaccacccc ccaggtcata aaccgcgata gtacggttgc
     2641 cagtgccttt gtccagaccg taagccagcg cagctgcggt cggttcgttg atgatacgtt
     2701 ttacttccag accagcgata cggcctgcgt ctttggttgc ctgacgctga gcatcgttaa
     2761 agtatgccgg tacggtgata acagcttcag ttaccggttc acccaggtaa tcttcagcgg
     2821 ttttcttcat ttttttcagc acttcagcag aaatctgcgg cggtgccatt ttctggcctt
     2881 taacttcgac ccatgcgtcg ccgttatcag cagcaataat tttgaacggc atgatggaaa
     2941 catcacgctg tacttcttcg tcctggaagc ggcggccaat caggcgttta atcgcaaaca
     3001 gggtgttttg cgggttcgtc actgcctgac gtttagccgg ctgaccaacc agagtttcac
     3061 catcctgggt ataggcaatg atagaaggcg tggtgcgatc gccttcggcg ttctccagca
     3121 cgcgaggaat ggtgccatcc ataatcgcta cacaagagtt ggtagtaccc aggtcgatac
     3181 caattatttt acccatctaa acgtctccac tatatattcg gtcatcatgt ggttgtgagt
     3241 ctgtaatagg ggcgaaacgt ctggtttcaa ctgcccaatt tcattttttt gcagactcac
     3301 tcactgcggt tgactactaa atggggtcgt aacccacgtc atcaaggggg agatgcaaaa
     3361 attttttgtg cttttgcgca gaaatttcgc gatatggcag gacaagacgt aaaattgaca
     3421 gcaattttat tcatataaag aatgaatcgc agccaaggca ttcatcaaaa aattgtaata
     3481 aaaagaaaag attacgtgcc tgaatcttct ctttatcagc agtaaactag tgggtattca
     3541 tccccctacc tcttcccact aagagaatcc ttatgaaatc cgtttttacg atttccgcca
     3601 gcctggcgat tagcctgatg ctgtgctgca cggcgcaggc aaacgaccat aaactcctcg
     3661 gcgtcattgc aatgccgcgt aacgaaacca acgatctggc gctgaaactt cctgtttgtc
     3721 gcattgtgaa acgcatacaa ctctctgccg accatggcga tttacagtta agcggtgcat
     3781 cggtttattt caaagccgcc cgtagcgcca gtcagagcct gaatattcct tcagaaataa
     3841 aagaagggca aaccactgac tggatcaaca ttaacagcga taacgacaat aaacgctgcg
     3901 tctcaaaaat caccttttcc ggtcatacgg tgaactcatc ggatatggcc acgctgaaaa
     3961 ttatcggcga cgactaacgc cagaatatag aagccacaaa aaatgaatgt taattacctg
     4021 aatgattcag atctggattt tcttcagcat tgtagtgagg aacagttggc aaatttcgcc
     4081 cgattgctca cccataatga aaaaggcaaa actcgcctct ccagcgtact gatgcgcaac
     4141 gaactgttta aatcgatgga agggcatccc gagcaacatc gccgcaactg gcagctgatt
     4201 gccggagaat tacagcattt tggtggcgat agtatcgcca acaaactgcg cggacacggt
     4261 aaattgtatc gggccatttt gctcgatgtt tcaaagcgat tgaagctgaa agccgacaaa
     4321 gagatgtcta cgtttgaaat tgagcaacag ttactggaac aatttctgcg taatacctgg
     4381 aagaaaatgg acgaggaaca taagcaggag tttctgcacg cggtcgatgc cagggtgaat
     4441 gagctggaag agctgctgcc gctgctgatg aaagacaaat tattggcaaa aggtgtgtcg
     4501 catttgcttt ccagccaact gacccgcatt ttacgcaccc acgcagcaat gagcgtactt
     4561 gggcatggtt tgctgcgcgg agcggggctg ggaggccctg tgggcgcagc attaaatggg
     4621 gttaaagcgg tcagcggcag cgcctatcgc gtgacgattc cagccgtact gcaaatcgcc
     4681 tgcctgcgcc ggatggttag cgccactcag gtctgatacc ggaaaccggg gaaaattttc
     4741 attttcccct caaaagatcg tagacactgc cccactggct gattattatg ccgcgccctg
     4801 aaaacactac agttattcag ggaaattatt tcaccattca ttcgatgatg atttttgagg
     4861 aattatgggc aacactaagt tggctaatcc ggcaccgctg ggcctgatgg gcttcggcat
     4921 gaccaccatt ctgcttaacc tgcacaacgt gggttatttc gctctggacg gtattattct
     4981 tgccatgggc attttctacg gcggcatcgc gcaaattttt gctggtctgc tggagtacaa
     5041 aaaaggcaac actttcggtt taaccgcatt cacctcttac ggttctttct ggctgacgct
     5101 ggttgcgatt ctgctgatgc cgaaactggg tctgaccgat gcgccaaatg cacagttcct
     5161 tggtgtctac ctgggtctgt ggggcgtatt tacgctgttt atgttcttcg gcactctgaa
     5221 aggcgcacgc gtcctgcaat tcgttttctt tagcctgacc gtgctgtttg ccctgctggc
     5281 gatcggtaac attgccggta atgccgcgat catccacttt gccggctgga ttggtctgat
     5341 ctgcggtgcc agcgcaatct atctggcgat gggtgaagtg ctgaacgagc agtttggtcg
     5401 caccgttctg ccgattggtg aatcccacta attgtcttga gatccccccg ctcgggggga
     5461 tttttttatt cgctaacgtc gcgtcttgca ctcttcggtc tgaatgcagc aaccacttct
     5521 ggtgccgttt caacgtatgg cccttccagc aactgaatgc agtacggtac gctggcaaaa
     5581 ataccatgca ccacaacgtt accctcagcg tccttcacac cttccagcgt ctctttaata
     5641 gacttcggct gaccgggtaa gttaaggatc agcgcctgtt tacgaatcac gcccacctga
     5701 cgcgaaagga tcgcagttgg tacaaaatgc aggctgatct ggcgcatctg ttcaccaaag
     5761 ccaggcatct cgcggtccgc tactgccagc gtcgcatcgg gcgttacgtc acgacgcgcc
     5821 gggccagttc cgcccgtggt gagcaccaga tggcaactca tttcatccac cagctcacac
     5881 aacgtttgct cgatgatcgc ctgctcatcg gggattaagc gggtttccag ttcaaacggc
     5941 gtggttagcg ccgatgtcag ccattcttcc agcgctggaa tgcctttatc ctgataaacg
     6001 ccgctggatg cgcgatcaga gatggaaact aagccaatac gtaaagtatt catcaagatt
     6061 tttccgttta aacagtctcg ttaaacagaa tgatacactg cgaagggagt gacagacagg
     6121 cttcagaaga ggtagcgtga ccgacttccc ggtcacgcta agaatgatta cagcagatcg
     6181 ccgatcattt tttccagttt ttcctggtca atagcaaact tacggatacc ttccgccagt
     6241 ttatctactg ccattggatc ctggttgtgc tgccacagga actcggactc agtgatacgc
     6301 gccggacgcg ctttcacttc gccggtgtaa gacagtttac gttcgatagc cccttcgctc
     6361 tccgccagct ctttcagcag tgccggtgcg atggtcagac ggtcacagcc tgccagttcc
     6421 agaatttcgc cgatgttacg gaagcttgcg cccataacca cggtttcata accgtgctct
     6481 ttgtagtact ggtagatttc agatacagaa accacgcccg gatcttctgc cggagcgtac
     6541 tctttcttat cggtattcgc tttgtaccag tcaagaatac ggccaacaaa cggcgagatc
     6601 aggaacacgc ccgcctccgc acaagcacga gcctgagcga aggagaacag cagggtcagg
     6661 ttacagttga taccttcttt ttccagctgt tctgcagcac ggataccctg ccaggtagaa
     6721 gccagtttga tcagaatacg atcgttgcta atacctgcat cgttgtagag tttgatcagg
     6781 cgttttgctt tcgcaattga cgcttcggtg tcataggaaa gacgcgcatc aacttcagtt
     6841 gagatacggc ccggaaccag tttcaggatt tccagaccaa tatttactgc cagtttgtcg
     6901 gtcgcgtcca cgatctgctg cgcgcgatcg ttgctctgct gtttcgccca ggcgacagca
     6961 tcatcaatca acttacggta ttccggaatc tgcgctgcgt taagaatgag agaagggttg
     7021 gttgtggcat cctgcggttg atacagcttc attgccgcga tgtccccagt gtcggccact
     7081 acggtggtgt actgacgaag ggaggtcaat ttgtccgtca tgatagtatt tctctttaaa
     7141 cagcttgtta gggggatgta accggtctgc cctgatgata tcacgacgca acgtcagcgc
     7201 aaccgccgac aagacggtta tcgccaggcg agactgtttc ggatttctga cactgcgttg
     7261 atatcgcccg ccatttttat acaaaacctc atgtatgcta cgcagaagtt atcaagtacc
     7321 tcgtagcgta tatacttctt aaacaattgg taacgtttac acaggaaagt catcgcgacc
     7381 ggcaataaga gggatatgca tgccagattt tttctccttt attaacagcg tcctttgggg
     7441 atcggtaatg atttacctgc tcttcggcgc aggttgttgg ttcacttttc gcaccggatt
     7501 tgtgcagttt cgctacatcc gccagtttgg caaaagtctt aaaaatagca ttcatccaca
     7561 gccaggcggt ttaacctcat ttcagtcatt gtgtaccagt cttgcggcgc gcgtgggtag
     7621 cggcaacctg gccgccgttg cgctggcaat taccgccggt ggaccaggtg ccgtcttttg
     7681 gatgtgggtt gctgcgttta tcggcatggc gacctcgttt gccgaatgtt cccttgcaca
     7741 actttataaa gaacgtgacg tcaatgggca gtttcgtggc ggaccggcat ggtatatggc
     7801 gcgcgggctg gggatgcgct ggatgggcgt tctgttcgcc gtctttttgc tcatcgccta
     7861 cggcataatt ttcagcggag ttcaggcgaa cgccgttgcc cgagccctga gtttttcttt
     7921 tgattttcct ccgctggtga cgggcattat tctcgctgtc tttgctctgc tggcaatcac
     7981 tcgcggtctt catggcgtcg cccggctcat gcagggtttt gtcccgttga tggcgataat
     8041 ctgggtactg accagcctgg tgatttgcgt aataaatatc gggcaacttc cccacgtcat
     8101 ttggtctatt tttgagagtg cttttggctg gcaggaagcg gcaggcggcg cggcgggata
     8161 taccttaagc caggcgatta ctaacggttt tcagcgcagt atgttttcca atgaggcggg
     8221 aatgggatcg acgccaaacg cggcagcggc agcggcagcg gcagcggcag cggcagctgc
     8281 atcctggcct ccgcatccgg cagcacaagg gattgtccag atgattggca tttttatcga
     8341 caccctggtc atctgtacgg caagcgccat gctgatatta ctggcgggta acggcacaac
     8401 ctacatgccg ctggaaggta ttcagcttat ccagaaggcg atgcgggtgc taatgggttc
     8461 ctagggtgct gaatttgtta ccctcgtggt tattctgttt gccttcagct ccatcgttgc
     8521 caactacatt tatgccgaaa acaatctctt ctttttacgc ctgaacaacc ctaaagcgat
     8581 ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc
     8641 gctgatgtgg caactggcag atatcataat ggcctgcatg gctattacca atttgaccgc
     8701 cattttactg ctctcgcctg tggttcatac cattgccagt gattatctac gccagcgtaa
     8761 actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg gatatcggtc gccagctttc
     8821 tccggacgcg tgggatgatg tttcgcagga gtaatcacaa ctatcgatca actcattctc
     8881 tttttttgct aaagtcggca taaatttcct gcaaggactg gatatgctga ttcttatttc
     8941 acctgcgaaa acgcttgatt accaaagccc gttgaccacc acgcgctata cgctgccgga
     9001 gctgttagac aattcccagc agttgatcca tgaagcgcgg aaactgacgc ctccgcagat
     9061 tagcacgctg atgcgcatca gcgacaaact ggcgggtatc aacgccgctc gctttcatga
     9121 ctggcagcca gatttcacgc cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga
     9181 tgtctacacc ggcttgcagg ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca
     9241 gcatttgcga atgctttccg gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc
     9301 ttatcgtctg gaaatgggga tccgtcttga gaatgcccga gggaaagatc tgtatcaatt
     9361 ctggggagat atcatcacca acaagctgaa cgaggcgctc gcagcacaag gcgataatgt
     9421 ggtgattaat ctggcgtcag atgagtattt taaatcggtg aagccgaaga aattgaatgc
     9481 cgagattatc aagccggtgt tcctcgatga gaagaacggc aaatttaaga tcatcagctt
     9541 ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa
     9601 accagagcaa ctgactggtt ttaatagcga aggttacttc tttgatgaag attcctccag
     9661 caatggcgaa ctggtgttta aacgctacga gcagcgttaa gtttgtagtc aatgccggat
     9721 gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga ttcaatatat tgcagaatta
     9781 acgtaggcct gataagacgc gccagcgtcg catcaggcgc tgaatgccgg attgttaccc
     9841 ggcatttgtc atttagcggt gatgtttgcc tggaccatga ccgccgtgat gatcatgagg
     9901 agctttctta tggtggcgcg gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg
     9961 ccattcataa tgttgtttcc accagccgtg gtcgcgccag tgacctccat cccagtaata
    10021 gccacgatta tcacgatcgc ctatctgtaa ttttactgac gggactaacg taatttccgc
    10081 agcctgtgct gccatgggag cgaccagaac cagggaaagt gcgagtacga tagattgcat
    10141 ctttttcaca ggttactcct tttatcattg cccgttatgg gtcgatatcc ggagattacg
    10201 cgatgagaac gacggttgtt attctccgca atcctaatct ctaagttacc gcatttattg
    10261 ataatttctc ctttttccct gtcgtttttc tccataattt cttcataaaa aagccgggct
    10321 gcataaaagc aaacccggcc tgattgagat aatgaataga ttttactgat gattcatcat
    10381 caatttacgc aacgcagaaa aatcggcggg cagattatgt gaaagcaagg gtaaatcagc
    10441 acgttctgcc agctcttttg gcagatccaa cgtttcaccg agaatcgctt ccacgctctc
    10501 tttaaatttc gccggatgcg cggtgccgag gaacaagcca tattcgcctg gattcaactg
    10561 gtcacgcagc gcacgatacg ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt
    10621 taactcacgc attgtctgtt gcgtggtttc atcatccacg gctgcataac ccagctcttt
    10681 cagttgccag attttgcggc ggaacaactc ttccacacgc ggccagttgt tcggctgact
    10741 cacgtccatc gcgttggata acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg
    10801 caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag
    10861 accgaatgac ttcgccagca gacccgccgt caaatcgccg aagtttccgc ttggcaccga
    10921 gacaaccagc tggttgcgcg cctcctgcgg cagctgcgca acagcttcaa agtagtagca
    10981 aatctgcgcc agcaaacggc tgatgttaat cgagttagcc gagtttaacc ccagcgccac
    11041 tttcagttct tcatcatcaa acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc
    11101 gtcgatggca acagtttcga tattgccgcc caacgtacag aacagttttt cttgcagtgg
    11161 actgattttg cctcgtggat agaggataac cactttcaca ttcggcaaac cgtagaaagc
    11221 atgagccact gcagctccgg tatcaccgga ggtcgcggtc agaatggtca ctggcttatc
    11281 atccgcaata tgggtcagca tttgtgccat aaagcgaccg ccgaaatctt taaatgccag
    11341 cgttggcccg tggaacaatt ccagacaacc gacatcgctt tcaacattgg cgaccggagc
    11401 cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt tcctgtggga tttcatcacc
    11461 aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc
    11521 aatttcagtc aggctgaatt ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc
    11581 caacccctgg gttacggctt gcgcaaagct gacctgctcg ttgtgatctt tcagattgta
    11641 gagtttcatt tagttttcca gtactcgtgc gcccgccgta tccagccggc aaatatgaac
    11701 aaaaccttcc tgattttgca ggtagttctt acccaaccag tcggcaacgc gctgggcggt
    11761 atccggcttg tcacatagag cgaacaaggt cgggccggag ccggagatac cgctcgctac
    11821 cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag ccaggcagta accgttcacg
    11881 gtagggttca gcgataacat ctttcatcag cttcgcggca agctcaggct gacgggaata
    11941 gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata
    12001 ctgcgccggt aaaatagccc gggcttctgc cgtcgagact ttaattcccg gatacgccag
    12061 cacccacagc cactcatcaa accctggcac ttgctggctg atgatgtcgt tttcttcgat
    12121 catcaactgc ataccaccaa gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga
    12181 gatacgtcct tccagctcgc ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc
    12241 gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc
    12301 cgaaccgatc ggcatattct tttccagagt catcgccact ggaatttgct tgccaagctc
    12361 ctggcaaaaa cgctcccagc actgataaac gatattttcc cgtggttctg acggcagctt
    12421 atcggcaaag cgtccgaggt tgttgagact gaatgtctct gccgcctcaa ccgtgactac
    12481 atctccgagc aatgcaccat caacgggtgc caccgccgcc ccgagcacat caaacccgac
    12541 gctcatattg gcactggaag ccggggcata aactttaacc atgtcagact cctaacttcc
    12601 atgagagggt acgtagcaga tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac
    12661 catatccgcg cagcaccaac ggcagcggct gataatagtg gctataaaag gccagggcgt
    12721 tttcgccatt tttcactttg aacagcggat cattcccatc cacttcggca atcttcacgc
    12781 ggcagacacc atcttcatca atattgccaa catagcgcaa aacttttcct tcatcacggg
    12841 ccttcgccac gcgcgcggca aagagatcgt cgagctgtga cagattcgcc ataaaagcgg
    12901 caacatcacc ctcggcgtta aactctgcgg gcagcacagg ttcaatttca atatccgcca
    12961 gctccagttc acgtcccgtt tcacgagcga gaatcaatag tttacgcgcc acatccatac
    13021 cagaaagatc atctcgcggg tccggttcgg tataacccat ttcccgcgcc agcgtggtcg
    13081 cctcggagaa actcatgcct tcgtctaact tgccgaagat ataagaaagc gaacctgaaa
    13141 gaatgccgga gaacttcacc aattcatcac ctgcattgag cagattttgc aggttctcaa
    13201 taaccggtaa tccagcccca acgttggtgt catagaggaa tttacgccgc gatttttccg
    13261 ccgcataacg caactgatga tagtaatcca tcgacgaggt gttggccttt ttgttcggcg
    13321 tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg atccgccact gcctggctgg
    13381 aggtgcagtc aacaatgacc gggttcagca gatgatattc tttcacgagg cgaattaagc
    13441 gcccgagatt aaacggctct ttggcttgtg caagttcttc ctgccagttt tccagattaa
    13501 ggccatgcac actagtgagc agagccttcg agttggcaac gccgcagaca cgtaagtcga
    13561 tatgtttatt cttcagccag ctttgctgac gcttcagttg ctccagcagc gcaccgccaa
    13621 cgccaccgac gccaatcaca aacacttcga taacctgatc ggtattgaac agcatctgat
    13681 gagtaacgcg cacgccagtg gtcgcatcat cgttatttac cacgacagag attgagcgtt
    13741 cagaagatcc ctgagcaatg gcgacaatgt tgatattggc gcgggccagc gcggcaaaga
    13801 atttcgccga gatcccacgc aaggtgcgca taccatcacc tacaaccgag ataatggcca
    13861 gccgttccgt caccgccagc ggctccagta agccttcttt cagttccagg tagaactctt
    13921 cctgcattgc ccgttcagct cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt
    13981 attcggaaga tgattgcgta atcagcacca cggaaatacg ggcgcgtgac attgcagcaa
    14041 agacgcgcgc cgccatgcca accatccctt tcatccccgg gccggaaacg ctgaacattg
    14101 ccatgttatt cagattggaa atgcccttga ccggtaattc gtcttcatca cggctggcac
    14161 caatgagcgt acctggtgct tgaggatttc cggtattttt aatcaggcaa gggatctgga
    14221 actgggcgat gggggtaatg gtgcgggggt gaagaacttt agcgccgaag taggaaagct
    14281 ccatcgcttc ctggtaggac atcgacttca acaacctcgc atcgggcacc tgacgcgggt
    14341 cgcaggtata gaccccgtca acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg
    14401 cagccagcac cgccgcggag tagtcggaac cgttacgtcc aagcaccacc agttcgcctt
    14461 tttcattacc ggcggtgaaa cctgccatca acaccatgtg atcagccgga atgcggcttg
    14521 ccgcaatacg gcgggtggac tcagcaatat cgacggtaga ttcgaggtaa tgccccactg
    14581 ccagcagttt ttcgaccgga tcgataacgg taacgttgtg accacgcgct tctaacacgc
    14641 cggccataat ggcgatcgac attttctcgc cacggcaaat cagcgcagcg ttgatgctat
    14701 ccgggcactg ccccaacaaa ctaatgccat gcaggacatg ttttatttgg gcaaattcct
    14761 gatcgacgaa agttttcaat tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg
    14821 tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt gggtaaagca tcctggccgc
    14881 taatggtttt ttcaatcatc gccaccaggt ggttggtgat tttggcgggg gcagagagga
    14941 cggtggccac ctgcccctgc ctggcattgc tttccagaat atcggcaaca cgcagaaaac
    15001 gttctgcatt tgccactgat gtaccgccga acttcaacac tcgcatggtt gttacctcgt
    15061 tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg
    15121 tacgcgtcag cccgcaccgt tacctgtggt aatggtgatg gtggtggtaa tggtggtgct
    15181 aatgcgtttc atggatgttg tgtactctgt aatttttatc tgtctgtgcg ctatgcctat
    15241 attggttaaa gtatttagtg acctaagtca ataaaatttt aatttactca cggcaggtaa
    15301 ccagttcaga agctgctatc agacactctt tttttaatcc acacagagac atattgcccg
    15361 ttgcagtcag aatgaaaagc tgaaaaatac ttactaaggc gttttttatt tggtgatatt
    15421 tttttcaata tcatgcagca aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc
    15481 caggcgttgc tgtaaccagt cgaccagttt tatgtcatct gccactgcca gagtcgtcag
    15541 caatgccatg actcgttcgc gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact
    15601 tttcgccggt tgttgtatta atgttgctaa ttgatagcaa tagaccatca ccgcctgccc
    15661 cagattgagc gaaggataat ccgccaccat cggcacacca gtaagcacgt cagccaacgc
    15721 taactcttcg ttagtcagcc cggaatcttc gcgaccaaac accagcgcgg catggctcat
    15781 ccatgaagat ttttcctcta acagcggcac cagttctact ggcgtggcgt agtaatgata
    15841 tttcgcccga ctacgcgcag tggtggcgac agtgaaatcg acatcgtgta acgattcagc
    15901 cagtgtcggg aaaactttaa tattatcaat aatatcacta gatccatgtg cgacccagcg
    15961 ggtggctggc tccaggtgtg cctgactatc gacaatccgc agttcgctaa aacccatcgt
    16021 tttcattgcc cgcgccgctg ccccaatatt ttctgctctg gcgggtgcga ccaggataat
    16081 cgttatacgc atattgccac tcttcttgac caaataaccg cgaacagggt gatcactgtc
    16141 aacttattac gcggtgcgaa tttacaaatt cttaacgtaa gccgcagaaa aagcccttta
    16201 cttagcttaa aaaaggctaa actattttct gactgcacta acggtttaat tgttaaaaaa
    16261 tgctacatat ccttctgttt acttaggata attttataaa aaataaatct cgacaattgg
    16321 attcaccacg tttattagtt gtatgatgca actagttgga ttattaaaat aatgtgacga
    16381 aagctagcat ttagatacga tgatttcatc aaactgttaa cgtgctacaa ttgaacttga
    16441 tatatgtcaa cgaagcgtag ttttattggg tgtccggccc ctcttagcct gttatgttgc
    16501 tgttaaaatg gttaggatga cagccgtttt tgacactgtc gggtcctgag ggaaagtacc
    16561 cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg
    16621 tactttagtc atgttacgcc gatcatgtta atttgcagca tgcatcaggc aggtcaggga
    16681 cttttgtact tcctgtttcg atttagttgg caatttaggt agcaaacatg cagaccccgc
    16741 acattcttat cgttgaagac gagttggtaa cacgcaacac gttgaaaagt attttcgaag
    16801 cggaaggcta tgatgttttc gaagcgacag atggcgcgga aatgcatcag atcctctctg
    16861 aatatgacat caacctggtg atcatggata tcaatctgcc gggtaagaac ggtcttctgt
    16921 tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat gttcctgact ggccgtgaca
    16981 acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt
    17041 tcaacccgcg tgaactgacg attcgtgcac gcaacctgct gtcccgtacc atgaatctgg
    17101 gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa gttcaatggt tgggaactgg
    17161 acatcaacag ccgttcgttg atcggccctg atggcgagca gtacaagctg ccgcgcagcg
    17221 agttccgcgc catgcttcac ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac
    17281 tgctgaagaa aatgaccggc cgtgagctga aaccgcacga ccgtactgta gacgtgacga
    17341 tccgccgtat tcgtaaacat ttcgaatcta cgccggatac gccggaaatc atcgccacca
    17401 ttcacggtga aggttatcgc ttctgcggtg atctggaaga ttaatcggct ttaccaccgt
    17461 caaaaaaacg gcgcttttta gcgccgtttt tatttttcaa ccttatttcc agatacgtaa
    17521 ttcatcgtcc gttgtaactt ctttactggc tttcattttc ggcagtgaaa acgcatacca
    17581 gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc accaccagca cactggttcc
    17641 caacaacagc gcgctatcgg cagagttgag cagtccccac atcactccat ccagcaacaa
    17701 cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc aataccgcct gcaaataaat
    17761 accgttcatt aacgccccaa tcagactggc gattatccat gccacggtaa aaccgatatg
    17821 ttcagaaagc gccagcaaga gcaaataaaa catcaccaat gaaagcccca ccagcaaata
    17881 ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt
    17941 cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc gttaattggt attgatcggc
    18001 tggcgtcgtc actgcgacgc taaacgcggg gaagttttcc cagccggtat cattgcctga
    18061 agcaaaacgc tcaccgagat tattagcaaa ccagctgctt tgccattgcg cctgaaaacc
    18121 tgactcgcta gcttcccgtt tggctggtag aaaatcacct aaaaaactgg gatgcggcca
    18181 gttgctggtt aaggtcattt cgctattacg cccggcaggc accacagaaa gatcgccggt
    18241 accgcttaaa ttcagtgcca tattcagctg caggttctgc ttccgccagt ccccttcggg
    18301 taaagggata tgcacgccct gcccgccttg ctctaacccg gtaccgggtt caatggtcag
    18361 tgccgttccg ttaacttcag gcgctttcac cacaccaata ccacgcgcat ccccgacgct
    18421 aatcacaata aagggcttgc ctaagatgat atttggcgcg ttgagctcgc taagacgcga
    18481 aacatcgaaa tcggctttta gcgttaaatc actgtgccag acctgaccgg tataaatccc
    18541 tatcttgcgt tcttccacgt tctgattgcc atcaaccatc aatgactcag gtaaccaaaa
    18601 atggataaaa cttcgtttcc gctccacggt tttatcctct tcctgcaccg tataaagctc
    18661 ggtcacagga atagcgataa gcggcccaac gagtttttgc ggcccgctgg tactttggcg
    18721 aatcgcatct tccacatcgc tacggtaatc ggcacgttcg acaatcactt gccgaatcag
    18781 cattatcgga atcaacaaca gcaatactgc accaaacaag gtggtcattt tccagaacag
    18841 gggggatttc aacatagtct tctcctttgc aatggcagca ggatacgaag gctatgtggg
    18901 aagaatttga agctatgtga agttacggtg aagtcgtagc gaggccagca cgccaccttc
    18961 ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc
    19021 caaccccaga ccgctgcttt tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa
    19081 aatacgtgac agcgcgtagt caggaatacc actaccggta tccagcacct taagcgtgac
    19141 gtgttcctga tccacttcgg cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat
    19201 ggtgttatcc agtaaattcc ccagcgcctg atccagtaac gccggttcag cagcaacatt
    19261 aacctcggtg ggcataacat gcaaagtgat gtttttttct gccaactgca cggtgcgcgc
    19321 ttcgctgacg cggcgaaata atgccgccac atcaacagca gtcagaacga cttcctgacg
    19381 attctccagt cttgcctggc gtagtaacgt ttccaccagc gcctgcattc gcgcattttg
    19441 tgtcagaatg ttgtcagtaa aacgagccac tacttcaggc ggcggacctt cgcgtaaaat
    19501 ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc tcatgggtta acgcataaac
    19561 atactgctca atatagtttt tcccttccag cttcacgcgc atactttcca gcgcctgcgc
    19621 gagtttacgc agctcgctac taccgagatc gggaagagga acgggcttat tgtcagtgac
    19681 ggaatcagca tagcgagtga gccgggcaat ggagcggttg atccaccaaa ccatgcctgc
    19741 gccaatcacc agtgcaatcc ccaacaaaat ggcgctggcc cataaaattc gccgctcgct
    19801 gcgtttaatg accggagcca tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag
    19861 ccgcgagccg cccataatcg gcgcggcaac atacatcacc gaactttcgg gatcggcagg
    19921 attttgcaac gtgctgcgcg caccatactg accacgcaac gttagccaga cgtcattcca
    19981 gcgcgaataa tcctgtccaa cggctttatt tgccgaatcg aacaatactt tgccatgcgc
    20041 atcggtcata tagacatgat attcattgcg cactttgtta atgccaccaa tattggcgcg
    20101 aaacgggcga tgttgtagct gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc
    20161 agagagcaaa tcgggacgcg ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc
    20221 ctccgttgct cttcgcacgc ccggtttaac ttctttgaca aaaatagcca gtacgaacca
    20281 ggccgccacc gccaccagta aaaaatagcc cagcagcaac cgcatgccga tacgcattac
    20341 aagcccctca ggctatatcc catgccgcga tgagtattaa tcggtgaaag atcggggttg
    20401 atggcgcgca gcttggcacg cagcgtttta atgtggatat cgacggtgcg atcgtaggtg
    20461 tcctgcgcat cttcccacac gctatccatt agttgctggc gggaccagac gcggcctggc
    20521 gacttgagta acgtcttcag caataaaaac tcataccgcg tcagcgttaa tggcgtgtca
    20581 aaccagctga tctgcgccgc gggttcattc aattcaaaat gaccaatacg gatgacggga
    20641 gacggcgtcg agaacttctt cacccgacgc agtaaggtgc gcaccctggc gcacacttcg
    20701 cggggtgaaa acggtttagc cacatagtcg tcaacgccaa tttccagccc aagcaggcga
    20761 tcgacctctt cacttcgggc cgtcaggaac agtacaggta acgccggatg gagcgccagt
    20821 aactggcggc acaattcaaa gccactgata tcaggcaaac caacgtcgag aatcatgacg
    20881 tcgggaacct gctggcgagc tttatccagc accggcaaac ctcgctcaaa gacctcgacg
    20941 gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc
    21001 actaaccaga ccgtttcccg ttgcataaat cgcctctgtt attgccgcca cggcatgaca
    21061 ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga
    21121 taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga cctgtaacga cttaaagacc
    21181 agggacgtgc gttttttgaa tactacctcg ccctgagctt tgccgttttt aatacgatcc
    21241 gacagttcaa tcggcccgac ttgctgacaa gaaatggccg catcggaggt atcttctgcc
    21301 aggcccaatc cccctttaat accaccggtt ttcgcccggc tcacataaca ggtgacattt
    21361 ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt gatccgggcc gatcatttta
    21421 aatacggtgt cgaccgaacc aatctcttct gcatgagcca atggccccag cattatcagg
    21481 cttaaagaca ggatcaaatg cttgtatttc atattgttac catttttatc tattacacga
    21541 gcaattagtt cgtcacggta aataacatat tgattaagaa caaaatctca attcttttat
    21601 ttccgtcagg ccctaaaaca tactctacta agaaaaaaac actgaatgct aaaacagcaa
    21661 aaaatgctat tatccaatta cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt
    21721 ttatggatca ggccggcatt attcgcgacc ttttaatctg gctggaaggt catctggatc
    21781 agcccctgtc gctcgacaat gtagcggcga aagcaggtta ttccaagtgg cacttacaga
    21841 gaatgtttaa agatgtcact ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt
    21901 cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat tctggacatc gcgctgcaat
    21961 accgcttcga ctctcaacag acatttaccc gcgcattcaa gaagcagttt gcccagactc
    22021 ctgcacttta ccgccgttct cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc
    22081 tgggtaaatt cacaatgcca gagcacaaat ttgtcaccct ggaagatacg ccgctgattg
    22141 gtgttaccca gagctactcc tgttcgctgg agcaaatctc tgatttccgc catgaaatgc
    22201 gttatcagtt ctggcacgat tttctcggca acgcgccgac cattccgccg gtgctctacg
    22261 gcctgaatga aacgcgtccg agtcaggata aagacgacga acaagaggta ttctatacca
    22321 ccgcgttagc ccaggatcag gcagatggct atgtactgac ggggcatccg gtgatgctgc
    22381 agggcggcga atatgtgatg tttacctatg aaggtctggg aaccggcgtg caggagttta
    22441 tcctgacggt atacggaacg tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg
    22501 atattgagcg atactacccg gcagaagacg ccaaagcggg agatcgccca attaatctgc
    22561 gctgtgaact gctgattcct atccgccgtt aacgctgcag ctcatctaat gcaggggcgt
    22621 ccagatgcga gatgtcccct gcggtttcaa cgacccagcc agacgccagc cacaggcttt
    22681 cctgataatc cacgcgcgaa atagaacagt tacgcagacg taagcggcgc tctgcccatg
    22741 ctggtaatcc gagaatcgta ctcaccagac atcccagtgc aataccatgg ctcaccagca
    22801 gcggtcggct tccctgcggt aagtcccggc aagattccag tgccgcattg acgcgatcgc
    22861 tgagctcctg cattgactcg ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct
    22921 gccgacgcca gttctcttct tcttcggtca gagaatcgat atgtcttttt tccagcacac
    22981 ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc acagccgcag gcctgggcga
    23041 tgatttccgc cgtacgccgg gtgcgtccta aatcgctact gataatatgc gtaatgccaa
    23101 gctctttggc acgggttgcc acctgcatcg cctgttgctc acctttggcg gtcagcgggc
    23161 tgtcagactg gccctgaata cgtcgctcgg cgttccactg cgtttcaccg tggcggacta
    23221 ggtatacctg taacatgctt tttttccgta tactgctgta acgttaattt tctgagtaat
    23281 gctgattatg caccaagttg tctgtgcgac caccaatccc gctaaaattc aggccattct
    23341 gcaggcattt cacgagatct tcggcgaagg atcctgccat attgcatccg ttgccgtcga
    23401 gagcggtgta ccggaacaac cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg
    23461 ggtagcaaat gcccgccgtt tacttccaga ggctgatttt tgggtggcga ttgaagctgg
    23521 cattgatggc gacagcactt tcagttgggt ggtgattgaa aacgccagcc agcgcggcga
    23581 ggcgcgttcg gcaactttgc cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga
    23641 ggcgcttggt cccgtgatgt cgcgttatac cggtattgat gagattggtc gtaaggaagg
    23701 ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc agcgtctatc accaggcggt
    23761 gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag gcattgtagg acggataagg
    23821 cgttcacgcc gcatccgaca ttcggtgcac gatgcctgat gcgacgctgc cgcgtcttat
    23881 caggcctaca aaatcaatcg cttttcagca acacctcttc cagccactga cgcagctcca
    23941 cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt cgcgatgccc gcgccgagtt
    24001 cattttttaa ctcacgctga ctcatttcgc cacgcaacag ctcttcgaca atacgcacgc
    24061 gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag gtttaacaac ggtaaatgga
    24121 gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg taaccactcc tggtgacgct
    24181 gttctgccat cgctgctgaa tagggtgatt gttgggccat aatatgtcgc cattgttagc
    24241 gggggaagca aaatgactcc cccggttgta ctcgctaaag agtacgatag catatcataa
    24301 acgtgcggat cagtaacgac gtccccattc cgtggcgctc atcaacgtcg gtttatcccc
    24361 catgaaatag cggtagtaag catcataagc cagcacgttc ttcacataac cgcgcgtctc
    24421 ggagaacgga atactctcga caaatgccac agcgtcgata cgcccggcgc tgttgccaag
    24481 ccaggttcgc acccgtccag gtccggcgtt ataagctgct gaggagaaaa tacggttatt
    24541 gccaaactgc tgataaacat attgcaggta actggtgcca atgttgatgt tcgtttccgg
    24601 atccagcaat tgcccagggc tgctataacc aggaatagag aacatcttca ccgtatgggt
    24661 cgctgtacca ggcataatct gcatcaggcc gctggcccct accggtgatt tcactttcgg
    24721 attccaggcg ctctcctgac gggcaatcgc catcgcatag ctttgcggga tctctttccc
    24781 actggtgtaa cgtttgaaaa gatcgttgta agccagcggg aatcgctctt ccagatgatc
    24841 ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc caccattggt tattgaaagc
    24901 ataccgagcc agttgagcct gctctgtttt tgacttgctc ttcaccagat tggcccactc
    24961 gctacgcgcg gtattatcga gattccagta catcaactcg cgcacgcgcg ccatctccgg
    25021 cccctgagtc agggtgctgt caacattctg cggcgcttta tcaatcttca gctcatactc
    25081 ttcaccgatg cgttgtgctg caaccatcgg gtagaaacca cgctgttgca tgagttgatg
    25141 caaaatctct ttcgcttcag cttcacgtcc gcgttccagc aataaatccg cctgccagta
    25201 acgccattca tctttctctt ttgcttccat cggcagacgc gccagccagg tattcaggcc
    25261 gcgacgatcg ccggtgccaa gcgccattcg cacacggcgt tcaatcaacg acgtggattg
    25321 cgagcgcata atggcgtcat cgcgccattt cgcctgctca tcggtgacat cattgcccat
    25381 caaacgccag gcgacgatat cgcgcagctc ctgaatctga tcttcattaa gctgctgcgc
    25441 ctgggcaagc gacgggatca tcagccgtgc attttcggca tcctgccgcg ccacactggc
    25501 aaacgccacc gccgccattt gacgggtaaa atcggtcgcg ccggttgtac gcgcgaaggt
    25561 cagtaccgta ttagggttgt tcgccagtga aatgattgcc gaggcgatag tctggtaatc
    25621 ggcaggcatc tgccctgcca gcactgttac caggcctgtg ttacccgctt tcatcgccag
    25681 acggatacgc tctaaatacg ccagcggatc ttgtttacct gacgcacgcc agacgctaaa
    25741 taacttgtca caggcgttag gctggctctt gccggttagc cacagctctt tcgccccttg
    25801 ccaggcttct tcactctgcc cggtgttcca tttcgcatag tagtaattac attgcgcttc
    25861 ggtagttccg ggcttttccg ggctaaaggc taacaagcca cgccagtctt cacgccgcgc
    25921 cagttcattg acgaaacgag attgcagcgt gcgagcggga ggaagcgtgg ggttagcgcg
    25981 aacaaagtta gtgaccgtca ccgccggttg attcatcagg tcatcggtga tctggcggta
    26041 ttccaggtag ggataaagcg gataatcctt cagtccaggc atcatttgtt cgaccacatc
    26101 catctgtcga ttatcccagg cctgcttgat ttgcgcgtaa cggctacgct gctcatccag
    26161 tgagtcggct cgcgccacgc tgctgaccgt cagcagacag acaccggcag ccaacagccg
    26221 ccaggtaact tgtttggctt tttccacaag cgcatcctct aagtgtaaat acatcaatgc
    26281 agcgtcatac cgcgtaatag atttatgcta accagtcatt gccgtttacg ccacgttacg
    26341 gacacttttt tacttttact gcgaaggtga tcgaccaact gggattaggc ggggaaacag
    26401 gctacacttc gcctttgaaa actcatcatc acgataaaca tagaggcgaa gtccaacgtg
    26461 gctcaattcg tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa acgtcatatt
    26521 ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa ttggtgtcct gggtctgaac
    26581 ggcgcgggta agtccaccct gctgcgcatt atggcgggca ttgataaaga catcgaaggt
    26641 gaagcgcgtc cgcagccaga catcaagatt ggttatctgc cgcaggaacc gcagctgaac
    26701 ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt cagaagtggt taacgccctg
    26761 aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg atgccgattt tgacaagctg
    26821 gccgctgaac aaggccgtct ggaagagatc attcaggctc acgacggtca taacctgaac
    26881 gtacagctgg agcgtgcggc ggatgcgctg cgtctgccag attgggacgc gaaaatcgct
    26941 aacctctccg gtggtgagcg tcgtcgcgtg gcgttgtgcc gcctgctgct ggaaaaacca
    27001 gacatgctgc tgctcgacga accgaccaac cacctggatg ccgaatccgt ggcgtggctg
    27061 gaacgcttcc tgcacgactt cgaaggcacc gtggtggcga ttactcacga ccgttacttc
    27121 ctcgataacg ttgcaggctg gatcctcgaa ctggaccgcg gtgaaggtat tccgtgggaa
    27181 ggtaactact cctcctggct ggatcagaaa gatcagcgtc tggcgcagga agcttcacaa
    27241 gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt gggtacgtca gggaactaaa
    27301 ggcagccagt cgaaaggtaa agcacgtctg gcacgctttg aagagctgaa cagcaccgaa
    27361 taccagaaac gtaacgaaac caacgaactg tttattccac ctggaccgcg tctgggcgat
    27421 aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggcg atcgcctgct gattgatgac
    27481 ctgagcttct cgatcccgaa aggggcgatc gtcgggatca tcggtccgaa cggcgcgggt
    27541 aaatcgaccc tgttccgtat gatctccggt caggaacagc cggacagcgg caccatcact
    27601 ttgggtgaaa cggtgaaact ggcatcggtt gatcagttcc gtgactcaat ggataacagc
    27661 aaaaccgttt gggaagaagt ttccggcggg ctggatatta tgaagatcgg caacaccgag
    27721 atgccaagcc gcgcctacgt tggccgcttt aactttaaag gggttgatca gggtaaacgt
    27781 gttggcgaac tttccggtgg tgagcgcggt cgtctgcatc tggcgaagct gctgcaggtt
    27841 ggcggcaaca tgctgctgct cgacgaacca accaacgacc tggatatcga aaccctgcgc
    27901 gcgctggaaa acgccctgct ggagttcccg ggctgtgcga tggttatctc gcacgaccgt
    27961 tggttcctcg accgtatcgc cacgcacatc ctggactacc aggatgaagg taaagttgag
    28021 ttcttcgaag gtaactttac tgagtacgaa gagtacaaaa aacgcacgct gggcgcagac
    28081 gcgctggagc cgaagcgtat caagtacaag cgtattgcga agtaatgcgt aaaatgccgg
    28141 atgcggcgcg aacgccttat ccggcctaca aaaccagcaa tttcaatacg ttgtaaaact
    28201 gtaggcctga taagacgcgg taagcgtcgc atcaggcatt gagcaccgaa tgccggatgc
    28261 ggcgtgaaca ccttttccgg tctacaacag ccttagcatc ctcaatttcg tggcgggtca
    28321 cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc ctcttccaga gaggttttca
    28381 actcatcaaa aaaactcatt ttatacctcg cctttgagca gtttcgtcac ggttatcctt
    28441 gctcccccat catctcccgc accagctcca cgcagcgcag gaaacggctg tcgtaatcgt
    28501 cctcttcaac ccgcacgaat tcgatattgt tctcttccag catctccacc agcaagttct
    28561 ggaactcttt gcgatccacc gaactgccga ggctgcgtaa accatccgcc acccacggcg
    28621 tgttgttctc cagcaggatc accagatcga aacggtattc atcaatcagc gcctgcacga
    28681 acggatgctc acgcccttcg tactttttgc agaacgcctg ggtagtgaca aaatcggtgt
    28741 caataaacgc cactttattg gcgtatttca ctgcaaaatc aatgtattgt gcgtggccca
    28801 acgcgatttt atcgtagtcg gaatactgca atgcgatctc atcaccgccg aggtgtgaaa
    28861 agacataatc gcggccatat tcccacgcac tggtggtgtt gaagatattg gcaagtttgt
    28921 ttaccagggt ggatttaccg ctcgactcgc cgccaaggat cgccacggta cgcacaaaaa
    28981 acggcttcac ttcggtagga atatattccc agtagcggaa cgggttttcg cggatctgcg
    29041 caccgctgat actcataaag gtacgtttcg gatcaaccag caccgtctcg atccccagat
    29101 gttccatata ctgtggcgca tcggcttctt ccgaggtgta gatcaggtcc ggctggatcc
    29161 ccttttcagc cataaacttt ttgatgccgt tgctccacac atcccagcca tgcggatacg
    29221 gctccatgcc ctcttcgttg aaagcatgaa tacggatatt tttctgatat ttaaaggttt
    29281 gcaatagcca gcgcagacga tccggcaccg tgggctgctg cgacatggcg ctgtcttcaa
    29341 acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat atgcagctcg tcaacctggc
    29401 tacaggcgcg ctggataagg tagatatgtc cggtatgcag cgggtagaac ttaccgaata
    29461 cgacaccaat ggttttcttc tgccggggaa attcaagccc caaaaaacgg tgcaatgcct
    29521 ccagcttttg cgcgctgggg cttttaattt tggcattcag taactggctt aaataccctt
    29581 tggtcatacc gctggcatca gctacctgct gtagcgtgca gccctgttgc ttgatggcag
    29641 ttttcaggta atcaaatgac gacatatctc cctccgtatc tctcattata agtcgtcgaa
    29701 cacgctaagc gcgtcggaga gttttttaac gccaaaaatc tgcatccctt ccggcgcttt
    29761 tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa ccgtgtttcg ccgcttcaga
    29821 aatccgctcc tggccactgg gcaccgggcg gatctcccct gccagcccga cttcaccaaa
    29881 caccaccaga tcctgtggca gcggtctgtc acgcaggctg gaaaccatcg ccagcagtaa
    29941 cgctaagtcg gcgctggttt cggttacctt cacgccgccg accacgttca caaacacatc
    30001 ctgatcggcc atttgcagac caccgtgacg gtgcaagaca gccagcagga ttgccagacg
    30061 gttttgttcc agccccactg ccacgcggcg cggattcgcc attatcgagt gatcgaccag
    30121 cgcctgaatc tccaccagca acggacgcgt tccttcccac accaccatca cggagctgcc
    30181 ggaagtcact tcatctccgc gacttaagaa aattgccgaa gggttgctga cttcacgcag
    30241 cccctgttcg gtcatcgcga agacgcccag ctcattcacc gcgccgaagc ggtttttatg
    30301 gctgcgtaag gtgcgaaaac gggagtcggc atcgccatcc agaagcaccg aacagtcgat
    30361 acagtgttcc agcactttcg ggccagccag cgaaccatct ttggttacgt gtccaaccat
    30421 gacaatcgcc acaccgcgcg ttttggcgaa acgcgtcaga taagccgccg tttcacgcac
    30481 ctgcgccacg ctaccaggcg atgattgtac atccgccata tgcatcacct ggatcgagtc
    30541 aattaccatc agcttcggtt gctcttcttc ggcaatcagg cagatctgct cgatgctggt
    30601 ttccgacaac atattgagat tgtcagtcgg caggccaagg cgatgagcgc gcattgccac
    30661 ctgttgcagc gactcttcgc cggtgacata cagcgttttc atctgctggg ccagtttgca
    30721 cagcgtttgc agcaacagcg tggatttccc cgcaccaggg ttaccgccaa tcagaatggc
    30781 acttcctggc accacgccgc cgcctagtac gcggtcgaac tctttaaatc cggtggaaaa
    30841 acgcggcagc tcttcaaggc tgatatcgga gagtttctgg acttttgcca ccccggcgct
    30901 accggcatag ccgctgagac gctcgttacg cgccaccgtt ggcgacgcag caagacgcac
    30961 ctcggtgatg gtgttccagg catgacaggc actgcactgc ccctgccagc gcggataatc
    31021 ggccccgcat tcattacaaa caaaggcgcg ttttggagct tttgccacgg tgtacctcgt
    31081 taatgctgtg ccgcccgcag gatgacgggc aggcaattac ttctgattca ggctgccaga
    31141 gaggatgcag aataccccca tcaggtcagc gtgacggatg gtgacttccg ccttttcatt
    31201 cacttttggc ttggcatggt aggcaatccc cagccctgct gctttgatca tcggcaggtc
    31261 attagctcca tcgccaatcg ccacggtctg cgccagcggg atttcatact cctgcgcgag
    31321 gcgagtcaga gttttcgctt tgtactgtgc gtctacgatg tcgccgatca cattgccggt
    31381 aaatttaccg tccatgatct ccagttcatt ggctagcacg gctgtcaggc gcagcttgtc
    31441 gcgcaggtat tcagcaaaga aagtaaagcc gccggaggca atcgccactt tccagcccag
    31501 cgtttccagc ttgagcacca gctgcgttaa gcctggcatc agcggcagat tttcacgcac
    31561 ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc acgcggctgc gcaggctggc
    31621 ggtaaaatcg agttcgccgc gcatcgcccg ttcggttacc tccgctacca tctcgcccgt
    31681 tccggccagc ttggcaattt catcaataca ttcaatctgg atggcggtgg aatccatgtc
    31741 catcaccagc aaacccggcg tgcgcaggtg cgggattttc cccagcgggg cgacatccag
    31801 ctgcgcttcg tgggccaggc gtgtagcccg tgcggtgagt gaacctgcca gacgaatcac
    31861 ctgataatct tccacgcacc aggcggcaac aatcaccatc gccgcaccca gtttgctctg
    31921 gtattgggtc agacgctgtt tatccagccc acgaccatac agcagccagc cgctacgagc
    31981 tgcgtggtaa tccagtggca tcacttcatc accacttaat gaaagaggca gacccggcca
    32041 taaagagaca tcttcaggca ggtcgcacca ggtaatgtta ggcattaagg ctcctgtaaa
    32101 atcgttcgaa gcagggaaaa taacgcatga ggctaccttg tatccattgc ttctggcaac
    32161 attaagtctc aaattttcaa agggtggaag atggctcgca caaaactgaa attccggcta
    32221 catcgggcag tgattgtcct gttctgtctt gccttgttag tggcactgat gcagggagcg
    32281 tcatggttta gtcaaaacca ccagcgacag cgtaatccac agctggaaga actggcccgc
    32341 accctggcgc gtcaggtgac gctgaacgtt gcaccgctga tgcgtaccga ctcaccggat
    32401 gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg aaagccgtat cctcgacgcg
    32461 ggtgtgtatg acgaacaagg cgatcttatc gcacgttctg gcgaaagcgt cgaagtgcgc
    32521 gatcggctgg cgctcgacgg taaaaaagca ggcggctatt ttaaccagca gattgtcgag
    32581 ccaattgcgg gtaaaaacgg accgctcggc tatctgcgcc tgacactcga cacccatacg
    32641 ctcgccaccg aagcccaaca ggtggataac accactaaca ttttacgcct gatgttgctg
    32701 ctctcactgg caatcggcgt agtgctgacc cgcacgcagc tacagggtaa acgcacccgc
    32761 tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg tgccggaaga ggaagaaagc
    32821 gagaaaaaag agtgacccat tactacaaga aaggaaatcg ttatgtccac attacgcctg
    32881 ctcatctctg actcttacga cccgtggttt aacctggcgg tggaagagtg tatttttcgc
    32941 caaatgcccg ccacgcagcg cgttctgttt ctctggcgca atgccgacac ggtagtaatt
    33001 ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc ggatggaaga agataacgtc
    33061 cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc acgatctcgg caatacctgc
    33121 tttaccttta tggctggcaa gccggagtac gataaaacta tctccacgtc gattgtgctc
    33181 aatgcgctga acgcgctcgg cgtcagcgcc gaagcgtccg gacgtaacga tctggtggtg
    33241 aaaaccgtcg aaggcgaccg caaagtctca ggctcggcct atcgcgaaac caaagatcgc
    33301 ggcttccacc acggcacctt gctactcaat gccgacctca gccgcctggc aaactatctc
    33361 aatccggata aaaagaaact ggcggcgaaa ggcattactt cagtgcgttc ccgcgtgacc
    33421 aacctcaccg agctgctgcc ggggctcacc catgagcagg tttgcgaggc cataaccgag
    33481 gcctttttcg cccattatgg cgagcacgtg gaagcggaaa tcatctcccc ggacaaaacg
    33541 ccagacttgc caaacttcgc cgaaaccttt gcccgtcaga gtagctgaga gtggaacttc
    33601 ggtcaggctc cggcattctc gcatctgctg gatgaacgct ttagctgggg cggcgtggaa
    33661 ctgcatttcg acgttgaaaa aggccatatc acccgcgccc aggtatttac cgacagcctc
    33721 aaccccgcgc cgctggaagc cctcgccggg cgactgcaag gctgcctgta ccgcgcggat
    33781 atgctgcaac aagagtgcga agcgctgttg gttgacttcc cggaccagga aaaagagcta
    33841 cgggagttgt cgacgtggat agcgggggcg gtaaggtaac tcgctgatta aaatatccaa
    33901 ggaaggaaat atgatcgatc tggaaaacca ggaacgggag attatcaatt taatgttcag
    33961 ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt
    34021 aagtaaaatg ttaggtatct cgattaattc tctaaaacag atcgaaaaga cggaacggct
    34081 tagctcaaac atcaaaagca aaatggcaga aatttatggt tgtccaccag agttgctgat
    34141 atgcccttcc tggatgacgg ctgaacataa atgaatagat tcgtgaatag atacttgaat
    34201 gtattttatt ttctatccat atgaaaaata atagtaaatc tataaatcag ttgctattga
    34261 tgaatagata ctaaaaagcc ggagcagtcc cccactccgg cttaacacga caatcgcctt
    34321 tcagcgaaac acaattactc tttatcgccc agcagaacgg attccagtgc gattttgatc
    34381 atgtcgttga aggtggtctg acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg
    34441 tcagacacgg tgcagatggt cagggctttc gcgccaaact ctgcagccac gccgtagata
    34501 ccagccgctt ccatttccac gccgaggatg ccgtattttt ccatcacgtc gaacatttcg
    34561 ccgtccggag agtagaacag gtcagcggag aacaggttac ccacgcgagc atcaacgccc
    34621 agtgctttag ctgcatctac tgcgttacgc accatgtcaa agtcagcgat agcggcaaag
    34681 tcatggtctt taaaacggat gcggttaact ttggaatcgg tgcaggcccc cataccgata
    34741 acgacgtcgc gcagttttac gtgcggcaga actgcgccac aggaaccaac gcggataatt
    34801 ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga tggagcagga cgggataccc
    34861 ataccgtgac ccattacgga aattttgcgg cctttgtaag taccagtgaa gcccagcata
    34921 ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag tttcagcaat atacttcgca
    34981 cgcagcgggt cgcctggcat caaaactacg tcagcgaaat cgcccatttc tgcattaatg
    35041 tgtggggtag ccattgtttt atcctttttg tgacataaca aaggcggagt caacgctccg
    35101 cccaaatcca tcagaacatg gctttgccat attccatatc agaagtacca aaatattttg
    35161 ccagagtctg gccgatatcc gcgaaggttt cacggtgacc cagtgagccc ggttttactt
    35221 tcgggccata taccagtacc ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg
    35281 gatcgcaacc gtggtcagcg gtgaggatca ggatgtcgtc atcgcgcagc agagacatca
    35341 gctccggcag acggcggtcg aacagttcca gacccgcagc ataaccggcg acgtcgcgac
    35401 ggtggcccca ggaagagtcg aagtcaacga agttggtgaa gacgatggtg ttatcacccg
    35461 cttctttcat ctctttgatg gtggcgtcaa acagcgcgtc caagccagtc gctttcactt
    35521 ttttggtgat accgcagttg gcgtagatgt ctgcaatttt accgacagaa accacctggc
    35581 cgtgtttttc atcaaccagt ttctgcagca cggtcggtgc tggcggctca acagccaggt
    35641 cgtgacggtt accggtacgc tggaagttac cagctttgtc accgataaac ggacgagcga
    35701 taacacgacc gatattgtag ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt
    35761 cgtagagttt atccagaccg aaagtttctt catggcaggc aatctggaac acggagtcag
    35821 cggaggtata gaaaatcggc ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa
    35881 tgaccgtacc ggaagagtgg cagttaccga ggtaacccgg cagattagcg cgttcgacca
    35941 gtttatccag cagctcttgc gggaagctgt tttcgtgatc ggagaaatat ccccactcaa
    36001 acagaaccgg gacaccggcg atttcccagt gaccagacgg ggtatcttta ccggatgaca
    36061 tttcgtgcgc ccatgcgtac gcgccgataa cttcagcgtt gccgtccatt cccgccggaa
    36121 tgaaaccggt agaaccttcg tgtgctttcg ccagccccag acggatcaga tttggcagat
    36181 tgagcggccc tttacgacca ttatcagctt cgcctttggc acaagcttct gcgatatgac
    36241 ccagggtgtc agccccgacg tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga
    36301 atgagtccag caccataata aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa
    36361 tttaaatgat cagatcagta taccgttatt cgctgatacg gcgatagaca gttggtgtgc
    36421 tttccggtgc gttatcggca agtttaattg ccgctttcac cgctttcgcc gcttcctgcc
    36481 agctgttttc gtctttcgcg aggataaccg ccagcggacg ctgaccgtct acatggtcgc
    36541 ccagacgcgc catatcagta aagccgacgc tgtaatcgat ggtgtcagat gcctgacgac
    36601 gaccgccgcc cattgcaacc actgccatcc ccagcgcgcg ggtatccatt tcactgacaa
    36661 aaccttcggt atcagcatag actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt
    36721 agttctcaac gaagtcggtc gggccttttt gtgccgctac catacgacca aagacttctg
    36781 ccgctttacc gttgtccagc accgcctgca atttcgcgcg cgcttcggcg tcatctttcg
    36841 ccagtttgcc ggagatcagc atctccacgc acagcaccat cgtgacatca aacagacgcg
    36901 ggttacgata ctcacccgtc aggaactgca ccgcttcacg aacttcaacc gcgttacctg
    36961 cactggaggc cagtacctga ttcatgtcgg tgagcagcgc ggtggtgcgc acgccagcgc
    37021 cgttagccac gccaacaatc gcttcggcaa gggcttcaga gagttcgtag gtcggcataa
    37081 acgcgccgct acccactttc acgtccatca ccagcgcgtc cagaccttcc gccagtttct
    37141 tcgccaggat cgaggcagtg atcagcggga tggagtccac ggttgcggta atatcacggg
    37201 tcgcgtagaa acgtttatca gccggagcca gtgaactggt ctgaccgata atcgccacgc
    37261 cgacgtcttt aataatttcg cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga
    37321 tggattccag tttgtcgagc gtaccgccag tatgaccgag gccgcgaccg gagatcatcg
    37381 gaatatagcc gccgcaggct gcgaccatcg gccccaacat cagcgaagtg acatcgccga
    37441 cgccgccggt cgagtgttta tcaacaatcg ggccattcag atgcaggctt ttccagtcga
    37501 gaacggttcc tgaatctcgc atcgccatgg tcagcgagac acgctcaggc attgtcatat
    37561 cgtggaagaa aatggtcatc gcgagggcgg caatctgccc ttcggaaata gtgttgtcgc
    37621 gaataccgtt gataaagaaa cgaatttctt catcgctcag cgcatgacca tcacgttttt
    37681 tacgaataat ttcttgtgcg agaaacaagg taacctcccg aggaaaagag tatgaggcgg
    37741 cttttgccgg atgcgtctac gccttatccg gcctacgggt agatacgtct cgtaggcctg
    37801 ataagcgcag cgcatcaggc gtaaagcatc ttacttagta gctgctggcg ctcttaccgt
    37861 cgccgtggcc cagcgctttc agcaggcttg ccagcaggct ggaagcacca aagcggtagt
    37921 gacgcgcatc agcccagtca gcaccgaaca gttcatctgc gatggcgaga tatttctgcg
    37981 catcttccgc agtacgcacg ccgcccgccg gtttgaaacc aacggttttt tctacgccca
    38041 tataacggat cacttccatc atgatgcgag cgctttccgg cgtcgcgttc acagccactt
    38101 taccggtaga ggttttgatg aagtccgcac ccgctttgat ggagatttca gacgctttac
    38161 ggatcagcgc ttcgtctttc agttcgccgg tttcgatgat cactttcagc agtacattcg
    38221 ctgccgcgca agcctcttta caggctttca ccaggtcaaa accaacctgc tcgtcacccg
    38281 ccatcagcgc gcggtacggg aacaccacgt caacttcatc agcaccgtag gcgattgccg
    38341 cacgggtttc tgccagcgcg atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg
    38401 tagcgatacg gatttccggg gtgccctgct ctttcagagt tttgcgagca atcgggataa
    38461 aacgaggata gatacagata gcggcggtat tgccgaccgg agttttggcc tgatgacaca
    38521 gagcaattac tttctcgtcg gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca
    38581 gtgcacgcag gctgcttgct ttcagatcag tcatttcatt ctcctggctt gtcgccaata
    38641 aaattcacct tgcgagtttg ttagtattct aacatctact ccgcaacaca cttcgataca
    38701 catcacaatt aaggaaatct acttacaagt ttgcatcact gtaatgcgat ctggttcaaa
    38761 taattcactt tcaaatgaat gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat
    38821 cacgaaactt cttacaatgg cgcatcacct tcggcataaa aggaaaagaa gatgcccact
    38881 tctcatgaaa atgcactgca acaacgttgc cagcaaattg tcaccagccc agtgcttagc
    38941 ccggagcaga agcgccattt tctggcactg gaagcagaaa acaatctgcc ttacccacag
    39001 cttcctgccg aagcccgccg cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt
    39061 catgcgccgt acaaaccgcg ctatgtctta cccgattacg ctcgttttct ggcgaacggt
    39121 tccgaatggc tggagctgga aggcgcgaaa gatcttgatg acgcactctc tctgctgacc
    39181 attctttacc accacgtacc gtcggtcaca tcgatgccgg tctacctggg gcaactggat
    39241 gcgttgttgc aaccgtatgt tagaattcta acacaaaacg agatcgatgt tcgaataaaa
    39301 cgtttctggc gttacctcga cagaaccctg ccagatgcct ttatgcacgc caatatcggc
    39361 ccgtctgatt cgcccattac ccgcgcgatc ttacgtgcag acgcgcaggt ttcaccgaac
    39421 ctgaccttta tctacgatcc tgaaatcacc cctgatgacc tgctgctgga agtggcgaag
    39481 aacatctgtg aatgtagcaa accgcacatc gccaacggtc cggtgcatga taaaattttc
    39541 acaaaagggg gctacgggat tgtgagctgt tacaactcac tgccgctggc gggtggtggc
    39601 agcacgctgg tacgccttaa cctgaaagcc attgccgagc gcagcgaatc gctggatgac
    39661 ttctttacgc gcactctacc gcactactgc cagcagcaga tcgccatcat cgatgcgcgg
    39721 tgtgaattcc tctatcaaca atcacacttc tttgagaata gcttcctggt gaaagaaggg
    39781 ctgattaacc ctgaacgttt tgtgccaatg tttggcatgt atgggctggc ggaagcggtt
    39841 aacttgctgt gtgagaaaga agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat
    39901 gaagtgggtt atcgcatcag cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa
    39961 tatggctggc aaaaacgcgc catgttacac gcacagtcgg ggatcagttc cgatatcggc
    40021 accacgccgg gcgcgcgttt gccgtatggc gatgagccag atccgatcac ccatctgcaa
    40081 actgtcgccc cgcatcatgc ttattattat tccggcatca gcgacattct gacgctcgac
    40141 gaaaccatca aacgtaaccc gcaggcactg gtacagcttt gcctcggtgc ctttaaagcc
    40201 ggaatgcgtg aatttaccgc caatgtcagc ggtaacgatc tggttcgcgt taccggttat
    40261 atggtgcgtt tgtcggattt agaaaaatat cgcgccgaag gttcacgcac caacaccacc
    40321 tggctgggcg aagaagccgc acgcaacact cgtattctgg aacgccagcc gcgcgtgata
    40381 agccatgaac agcagatgcg ctttagtctg taagattatc cccttctcct gcgttgacgg
    40441 gccaggcagt cgtctggctc tgtttttgca gggctgcaat ctgcgctgca aaaactgtca
    40501 caatccgtgg acgatgggac gttgcaatga ctgtggggag tgcgtgccac agtgtccgca
    40561 tcaggcgttg cagattgttg acggcaaagt ggtgtggaac gctgtggtct gcgagcaatg
    40621 tgatacctgc ctgaagatgt gtccgcaaca tgccacacca atggcgcaat ccatgagcgt
    40681 ggacgaagtg cttagccatg tgcgcaaagc ggtgctgttt attgaaggga taacggtaag
    40741 cggcggcgaa gtcacgaccc aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa
    40801 cgatccgcaa ctgcgccatc tcacctgtct ggtggacagt aacggtatgt tgagcgaaac
    40861 cggctgggaa aaattactcc cggtgtgtga cggcgcaatg ctcgatctca aagcatgggg
    40921 tagcgaatgt catcaacaat taaccggacg cgataatcaa cagattaagc gcagcatcta
    40981 tttgctggca gagcgcggca agctggcgga actgcgtttg ctgatgatcc caggccaggt
    41041 ggattatttg caacacatcg aagaactggc ggcgtttatc aagggacttg gagatgttcc
    41101 ggttcgcctg aacgcgtttc atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag
    41161 cgccacgccg gaagacgttg agccgttggc tgatgcatta aaggtgcgcg gggtgagccg
    41221 gttgatattt ccggcgctct atttgtgaca tgccggatac ggcctacggt tcggcacaga
    41281 cttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga
    41341 tgcggcgtga acgccttatc cggcctacgg ttcggcacaa acctgtaggc ctgataagac
    41401 gcgttaagcg tcgcatcagg cattatgcgc cgactgccgg atgctgccta cgaagggcaa
    41461 actacccacg aacgttaaac accgcatacg tgttattaag caacacttcg gcaatctcat
    41521 ccgcgggttc cgggcgtaac tcgcaaagca cggcaaacac acgggcagcc tgttccgggc
    41581 ggttaggctg cccctgaaaa ccgttgagcg gcatatccgg cgcatcggtt tccagcaaca
    41641 acgatgccag cggtaatttt gcgatgacat cgcgggtttt actggcgcgt ggataggtga
    41701 tagtaccgcc tacgccgatt ttgtagcccg gctgtacaaa ccgttcagcc tgttgctggc
    41761 tgccagaaaa tccgtggaca acaccagcac gcggcaaatc gtggcgttta agatgcatcg
    41821 ccagtttgtc gtgcgtgcgc cgtgaatgca ggatcaccgg cagatcgtag cgtttcgcca
    41881 gtttcagttg ttcgtcgagt aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga
    41941 gatccagacc gatctccccc accgccacca ccttcgccgg acgcctttcc agcgcctgct
    42001 gtagctgctc aagagacaca tcgctatgtt tttccaacat accaggatgc aagcccaatg
    42061 cggcatacag cggttgataa ttttccgcta acgcctgcac gcgggcaaaa ttttccgcct
    42121 ctgtcgccgg aacaatgatc ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct
    42181 cttcatcgcc aataaaaggc gggaaatcaa aatggcagtg agtgtcgata aaacggcaaa
    42241 tcaagccaga tcctcattgt taaatgtcgg gtcgttcgcc tgcggagcgt cgctcacttc
    42301 ggcaaccagc gtatcgttgg cgactggcgc aggcggaatg acaatcgatt ccggcgtcac
    42361 cactggcacc agatggcggg taagcggcgc tttttcagtt aatagcttgc caaccgtggc
    42421 gaggaaataa cgaccgcata aacgcccaag tttatagtct tcacgcagcg ccggaatccg
    42481 actgccaagc gcgatactat gtaatggctt cggcggataa atttcggata tccgcagctt
    42541 gcccggtggt ttctcaataa attgctgaat gtcacgatag ctggtttcat gatgctgcac
    42601 cagattgacc agcggctgca ggctactgtc acccagccag cgttccatgc gtttgaacca
    42661 ctgcggcgtg tagtacattt gtgacggcac agtgcggatg acgaccaacg ttttagcgcc
    42721 ctgccttgcc gcctctttaa ccggaatcgc atcactgatc ccgccatcca ggtagttaat
    42781 gccttccagc gacactccgc tacgataaaa gccaggtatc gcactggagg cgcgaatcac
    42841 atccagccag ttttgtttgg ttggtaaaaa gtaattcggc gcgtagtcat cctgacgaca
    42901 ggcgcacata taaaacgatt tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa
    42961 cggcatctgg cttgcggtgg cctccaccag ccagtcgaga tcgataagat ttcctccacg
    43021 gacaaagcgc aatggatcga aaaattcgcg ttttgtggta tagcgcatga tgactttgcg
    43081 cgcgtaaccg ggctgattgc agataaacgc cgagaggttc tgcgccccgg cagatgtgcc
    43141 gagataaaga tcgaaaggat taaactgcgc gcgcataaac tcatccagca cgccagccgt
    43201 gaaaattcca cgctgtccgc cgccttcgca caccagagct attcgtccag gctggaacgg
    43261 ccttagcgac aacggcgcaa tattaccaag cgttacaggt attcgctgcc ccacttctgc
    43321 cttcctgttt ttgttgtgaa agaaagacaa agtaacgcaa tttattattc gctaaaaccg
    43381 caaaagccag tccgctggac tggctttgga taaaattttc ttaatggtct gaaatctagg
    43441 gtcgttttcg gcccatgaac aaactcacta ggaacagaat aatcccgacg acaaagacaa
    43501 ttttagctgc gcctgcagcg gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta
    43561 acgcgataac cagaaatatg atgccccaac gaaacataag cgtctccttt accatagtta
    43621 atgtcaccgc ttgatatgag cgtgaaaatc actcaatgcg atatttttag tgtggtgcac
    43681 atcgcgcctc ccgacaaagt tcgggaggac gaattacgac gaattactta gttttcagat
    43741 catttttaac gcttttcaca ccatctaccg ctttggcgat actttcagca cggtcacttt
    43801 gtgcctgaga atctacggta ccggagagct gaaccacgcc atcggtggtt tcaactttca
    43861 ctttacgtga agggacgata tcgtccgcca gcagtttggc tttgatttca ctggtggtgg
    43921 cggtgtcacc cgcgtagtcc ttcaccgagc cttctttagc gtcgcgaacg tgcagtttgt
    43981 cgctgacaga ggtcacgcct tcaacgcctt tcgccacttt cactgcctct tcggcctggg
    44041 cctgactttc aacgaaaccg ctcagggtca cgactttttg atcggttttg acagagatat
    44101 cggtgctctt gatgttgtca tgatccacca gcgccgcctt cactttcgcg gtgatggcgc
    44161 tgtcatccat gaaattaccg actttattca tagagctatc gactttttgc cctgcgcttt
    44221 cattggtagt ctgcgcgttg ttttccgcgt aggcagagcc ggtcgcgacg gcagaggtca
    44281 acattacagc cagcagagtt ttcgaaatct tcagtcttgt catagtcatc gatttattcc
    44341 tgtatgtttg ctcgtaattt gagcctagca acacgaggtt gcattgctga atagggagag
    44401 acttcaccct ctacagaagt caatggtcgc catcacaaaa gcgatgggtg atgaataacg
    44461 accattacag tctctgaatc agttattaat atcggtagaa tgacaatcga cggctttaga
    44521 tactgatatc tacgcattga acggtattta acgccatcag aaatgtcatc actttgttaa
    44581 atatagatca caattttgaa accgctcggg atatcagcga aaacataagc aaaagtgaat
    44641 gttttaagaa cattccgtaa gcggctaata aggaagggaa attgacaggg cgcagcggct
    44701 accgcgccct ggaggcaaga attaatgctc gcgggtctgg tggaactgaa cgtccggata
    44761 acgttcctgt gccaggcgca ggttgaccat gctggtagcg atgtaagcga ggttatcgcc
    44821 gccatcaagc gccagttggc tttcgttctt acgcttgaac tcttcgaatt tcttcgcgtc
    44881 tgcacattct acccagcggg cagtggcaac gttgactgac tcatacactg cttcaacgtt
    44941 gtattcgctc ttcaggcgcg ctaccaccac atcaaactgc agcacaccaa ccgcaccaac
    45001 gatcagatcg ttgttggaga ttggacggaa cacctgcacc gcgccctctt cggaaagctg
    45061 taccagccct ttgagcagct gtttttgctt cagcggatct ttcaggcgga tacgacggaa
    45121 cagttctggt gcgaagttcg gaataccggt gaacttcatc atctcaccct gggtaaaggt
    45181 gtcgccgatc tgaatggtgc cgtggttgtg caggccgagg atatcgcccg gatacgcttc
    45241 ttcaacgtgc gaacggtcac ccgccataaa ggtcagcgcg tcggagatca ccacatcttt
    45301 cgcagtgcgc acctggcgca gtttcatgcc tttttcatat ttaccggaca ccacacgcat
    45361 aaacgccacg cggtcgcggt gtttcgggtc catgttggcc tgaattttaa atacgaagcc
    45421 ggtaaattta tcttcgctcg cttctacggt acgggtatcg gtctgacgcg gcatcggcgc
    45481 aggcgcccac tccaccaggc cattcagcat atgatcaacg ccgaagttac ccagcgcagt
    45541 accgaagaat accggagtga tttcgcccgc aaggaacagc tctttgtcga actcgttaga
    45601 cgcgccttta accagttcca gttcgtcacg cagctgctgt gccagatctt caccaaccgc
    45661 agcatcgaga tccgggttat tcagcccttt aacaatgcgg acttcctgaa tggtgtgccc
    45721 tttaccgctc tgatagagat aggtttcatc tttataaagg tggtaaacgc ctttaaacag
    45781 cttgccgcag ccaattggcc aggtgatcgg tgcgcagcca attttcagct cgttctcaac
    45841 ttcatcgaga agttccatcg ggtcgcggat atcacggtca agtttgttca taaaggtgag
    45901 gatcggcgtg tcgcgcagac gggtaacttc catcagctta cgggtacgat cttcaacacc
    45961 ttttgcggcg tcgataacca tcaggcagca gtccaccgcc gtcagggtac gataggtatc
    46021 ttccgagaag tcttcgtgcc ccggggtgtc gagcaggtta accaggcaat cgtgatacgg
    46081 aaactgcatc acagacgtag taatggagat cccacgctgc ttttccatct ccatccagtc
    46141 cgacttagcg tgctggttgg aaccacggcc ttttactgta ccggcggtct gaatggcctg
    46201 tccgaacagc agcaccttct cggtgatggt agttttaccg gcgtccgggt gagaaataat
    46261 ggcaaaagtg cggcgcttcg ccacctcttg caaataagga gacaacgtca taattctttc
    46321 ttcttgagta agcggcatcg cgccgcgctt gttgaaaacg aaaaattgcg gctattttac
    46381 ccatcaacgg gggggaggca atcagtgttt acacaggagc tgctccagtt cgtgcaacga
    46441 agaaacggtc caggtgggcg cgatgccttc tggttgctcg cgattgtgcg cattcagcca
    46501 gcaggtcgca agcccggcgt tgatgccacc gagaatatcg gactcggcag tgtcgccaac
    46561 catcagcacg cgtgaacggt caggattgcc cgcctgttcc agcgcataat cgaaaatttt
    46621 cttattcggt ttggcaacgc caacttcttc ggaaatcacc agcaaatcga agtaatcacg
    46681 caggcccgtc cgttccagac gcacttgctg caaggcgcta aaaccgttgg tgatgatgcc
    46741 gattttagcg ttgccacgaa tggcgttaag cagagaaacc gcgcccggca acggcgtaca
    46801 gatttccgcc atcgcattaa taaaggcatc gttaagcaag cctgcttcaa ctttcagccg
    46861 ttcggcccag ctctcgaaac gcccgtgctg aagctgtaat gaagtgatcg cgccgttttg
    46921 ataatccacc cacagtggct tgttaacggc ctgatagtcc tgaaaatctt cagcggtaaa
    46981 ggtgacgctg taatcaagaa acatccgctg caggccggtg aatgagtcaa aggtaaacag
    47041 cgtttcatcg gcatcaaaga aaatccagtc ccacttcatt attccacctt gtcttacata
    47101 ctgattggca acgccatgat gatggcgtct tcgcgaccgt ccgtggtggg gtagtaattg
    47161 cggcgaatcg tcgcctcgtt aaagcctaaa ctttcgtaca gggcaatggc ggcagcattt
    47221 gaggcacgga cttccagcca tagtgtcgcc acgccgcgtt tttccagttc gtcgatcaga
    47281 tgttccagca gcgccctacc cagcccctga cgctgatagt cgggatcgac cgcaatattg
    47341 aacaatgtag cttcatccaa caccacttgc gtaatcgcaa acgccgccat tttgccgttt
    47401 tgcgttaact gaaagttgag ataacgctcg ccctggttgc tggcaaaggt tttttcactc
    47461 cacggaaagg cgtgggcgcg ttgttcaatg tggtaagccg ctggtaaatc agtcgtttcg
    47521 agggaagaaa tcgtgttcat atgtgcaaat ttgttgccat aacgcggcgc gtgccgttgg
    47581 gtttacccgt aattcggtga gcgccggtga tgccacctga gcgccttcca atgatagcgg
    47641 ttcgtcagta cccaaccgcc agctgttgca gcgactgcct tgcggcagca tcgcgatttt
    47701 ttctggcgtc agttgcagca cttgatcggg gctgacggtt aatgcgcgca gaacatcgct
    47761 cactaaagga tcagtcaggg cgggaagatc gtttgccacc atcaccagac ggacgtgtgc
    47821 cgggctcgca atggcaatct ccccctgcaa cgcgccaggg cgacgcagcg accactgggt
    47881 aatgcccagt tgctgtaact gccagtctcg tcgggatgtc atagcgaatc gctcctgttg
    47941 tcaggggcgc aaatatagca aattcgtcga taccgcgcca acatatggct ataatcgccg
    48001 ccagtatcaa ttgaggagca ttccatgtct gcatttaccc cggcaagtga agtcttgctg
    48061 cgtcacagtg atgatttcga acaaagccgt attctgtttg ccggagactt acaggatgac
    48121 ctgcccgcgc gtttagatac cgcggccagc cgtgctcata cccagcaatt ccaccattgg
    48181 caggtgttaa gctgccagat gggggataac gcccgtttta gtctggtcgc cacggcgaat
    48241 gatgtcgcag attgcgatac gctgatttac tactggccga agaacaaacc ggaagcccag
    48301 ttccagttga tgaatttact ttctctgctg ccagtgggga cagatatttt tgtcgttggc
    48361 gagaaccgca gcggcgtgcg cagcgctgag cagatgctgg cagattatgc gccgttgaat
    48421 aaagtcgaca gcgctcgtcg ctgtggcctc tattttggtc gtctggaaaa acagccagta
    48481 tttgatgcgg ataaattctg gggcgaatac agcgtcgatg gcctgacggt caaaacgctg
    48541 cctggcgtgt ttagccgcga cggtctggat gtcggtagcc agttgctgct ctcgacgtta
    48601 actccgcaca cgaaaggtaa agtgctggat gtcggctgtg gcgcgggtgt actttcagtt
    48661 gcctttgcgc gccattcgcc gaaaattcgt ctcaccttgt gcgatgtctc tgccccggcg
    48721 gtagaagcca gccgcgcaac acttgcagcc aacggtgttg aaggtgaagt ctttgccagc
    48781 aacgtctttt ccgaggtgaa aggttgtttt gatatgatca tctccaaccc gccgttccac
    48841 gatgggatgc aaaccagcct ggatgcggcg caaacgctga ttcgtggcgc ggtgcgtcat
    48901 cttaatagcg gcggcgagct gcgaattgta gcgaacgcct tcctgcctta cccggacgtg
    48961 ctggatgaga catttggctt ccatgaagtt atcgcgcaaa ccgggcgctt caaggtatat
    49021 cgcgccatta tgacccgcca ggcgaagaaa ggttgagtat ctgatatctg tcgccgcata
    49081 ccgtgtcgga tgcggcgtga acgccttatc cgacctacaa aacctgcacg ctagtttcct
    49141 gatggacatt tttccagcaa ttacacatca ggcgataatt aactattgac gaaaagctga
    49201 aaaccactag aatgcgcctc cgtggtagca attcttttta agaattgatg g
//
LOCUS       DADLZM010000013        44381 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLZM010000013 DADLZM010000000
VERSION     DADLZM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.013"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..213
                     /locus_tag="JNY73_000766"
     CDS             <1..213
                     /locus_tag="JNY73_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY8046877.1"
                     /translation="RPISKNAGDYQAGDIVSWRLDNGLAHIGVASDGFARDGTPLVIH
                     NIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(240..1178)
                     /gene="yijE"
                     /locus_tag="JNY73_000767"
     CDS             complement(240..1178)
                     /gene="yijE"
                     /locus_tag="JNY73_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY8046878.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1239..3419)
                     /gene="katG"
                     /locus_tag="JNY73_000768"
     CDS             complement(1239..3419)
                     /gene="katG"
                     /locus_tag="JNY73_000768"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY8046879.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(3748..4638)
                     /gene="metF"
                     /locus_tag="JNY73_000769"
     CDS             complement(3748..4638)
                     /gene="metF"
                     /locus_tag="JNY73_000769"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY8046880.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(4987..7419)
                     /locus_tag="JNY73_000770"
     CDS             complement(4987..7419)
                     /locus_tag="JNY73_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY8046881.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7422..8582)
                     /gene="metB"
                     /locus_tag="JNY73_000771"
     CDS             complement(7422..8582)
                     /gene="metB"
                     /locus_tag="JNY73_000771"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY8046882.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            8859..9176
                     /gene="metJ"
                     /locus_tag="JNY73_000772"
     CDS             8859..9176
                     /gene="metJ"
                     /locus_tag="JNY73_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY8046883.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9360..9968
                     /locus_tag="JNY73_000773"
     CDS             9360..9968
                     /locus_tag="JNY73_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY8046884.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(10029..10241)
                     /gene="rpmE"
                     /locus_tag="JNY73_000774"
     CDS             complement(10029..10241)
                     /gene="rpmE"
                     /locus_tag="JNY73_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY8046885.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            10444..12642
                     /gene="priA"
                     /locus_tag="JNY73_000775"
     CDS             10444..12642
                     /gene="priA"
                     /locus_tag="JNY73_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY8046886.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            12798..13823
                     /gene="cytR"
                     /locus_tag="JNY73_000776"
     CDS             12798..13823
                     /gene="cytR"
                     /locus_tag="JNY73_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY8046887.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            13915..14874
                     /gene="ftsN"
                     /locus_tag="JNY73_000777"
     CDS             13915..14874
                     /gene="ftsN"
                     /locus_tag="JNY73_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY8046888.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            14967..15497
                     /gene="hslV"
                     /locus_tag="JNY73_000778"
     CDS             14967..15497
                     /gene="hslV"
                     /locus_tag="JNY73_000778"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY8046889.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            15507..16838
                     /gene="hslU"
                     /locus_tag="JNY73_000779"
     CDS             15507..16838
                     /gene="hslU"
                     /locus_tag="JNY73_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY8046890.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            16905..17831
                     /gene="menA"
                     /locus_tag="JNY73_000780"
     CDS             16905..17831
                     /gene="menA"
                     /locus_tag="JNY73_000780"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY8046891.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            17924..18409
                     /gene="rraA"
                     /locus_tag="JNY73_000781"
     CDS             17924..18409
                     /gene="rraA"
                     /locus_tag="JNY73_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY8046892.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(18494..18739)
                     /gene="zapB"
                     /locus_tag="JNY73_000782"
     CDS             complement(18494..18739)
                     /gene="zapB"
                     /locus_tag="JNY73_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY8046893.1"
                     /translation="MTMSLEVFDKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            19165..20010
                     /gene="glpF"
                     /locus_tag="JNY73_000783"
                     /pseudo
     CDS             19165..20010
                     /gene="glpF"
                     /locus_tag="JNY73_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            20050..21540
                     /gene="glpK"
                     /locus_tag="JNY73_000784"
     CDS             20050..21540
                     /gene="glpK"
                     /locus_tag="JNY73_000784"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY8046894.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            21770..22780
                     /gene="glpX"
                     /locus_tag="JNY73_000785"
     CDS             21770..22780
                     /gene="glpX"
                     /locus_tag="JNY73_000785"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY8046895.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            22877..23623
                     /gene="fpr"
                     /locus_tag="JNY73_000786"
     CDS             22877..23623
                     /gene="fpr"
                     /locus_tag="JNY73_000786"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY8046896.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(23628..24056)
                     /gene="uspD"
                     /locus_tag="JNY73_000787"
     CDS             complement(23628..24056)
                     /gene="uspD"
                     /locus_tag="JNY73_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY8046897.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(24083..24382)
                     /locus_tag="JNY73_000788"
     CDS             complement(24083..24382)
                     /locus_tag="JNY73_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY8046898.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAAFSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(24594..25034)
                     /locus_tag="JNY73_000789"
                     /pseudo
     CDS             complement(24594..25034)
                     /locus_tag="JNY73_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            25135..25734
                     /locus_tag="JNY73_000790"
     CDS             25135..25734
                     /locus_tag="JNY73_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY8046899.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            25842..26609
                     /gene="tpiA"
                     /locus_tag="JNY73_000791"
     CDS             25842..26609
                     /gene="tpiA"
                     /locus_tag="JNY73_000791"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY8046900.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(26664..27419)
                     /gene="cdh"
                     /locus_tag="JNY73_000792"
     CDS             complement(26664..27419)
                     /gene="cdh"
                     /locus_tag="JNY73_000792"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY8046901.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(27526..28515)
                     /locus_tag="JNY73_000793"
     CDS             complement(27526..28515)
                     /locus_tag="JNY73_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8046902.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(28835..29797)
                     /gene="pfkA"
                     /locus_tag="JNY73_000794"
     CDS             complement(28835..29797)
                     /gene="pfkA"
                     /locus_tag="JNY73_000794"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY8046903.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(29978..30880)
                     /gene="fieF"
                     /locus_tag="JNY73_000795"
     CDS             complement(29978..30880)
                     /gene="fieF"
                     /locus_tag="JNY73_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY8046904.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(31028..31528)
                     /gene="cpxP"
                     /locus_tag="JNY73_000796"
     CDS             complement(31028..31528)
                     /gene="cpxP"
                     /locus_tag="JNY73_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY8046905.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            31678..32376
                     /gene="cpxR"
                     /locus_tag="JNY73_000797"
     CDS             31678..32376
                     /gene="cpxR"
                     /locus_tag="JNY73_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY8046906.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            32373..33746
                     /gene="cpxA"
                     /locus_tag="JNY73_000798"
     CDS             32373..33746
                     /gene="cpxA"
                     /locus_tag="JNY73_000798"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY8046907.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(33852..34526)
                     /gene="yiiM"
                     /locus_tag="JNY73_000799"
     CDS             complement(33852..34526)
                     /gene="yiiM"
                     /locus_tag="JNY73_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY8046908.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(34675..35658)
                     /gene="kdgT"
                     /locus_tag="JNY73_000800"
     CDS             complement(34675..35658)
                     /gene="kdgT"
                     /locus_tag="JNY73_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY8046909.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(35918..36538)
                     /gene="sodA"
                     /locus_tag="JNY73_000801"
     CDS             complement(35918..36538)
                     /gene="sodA"
                     /locus_tag="JNY73_000801"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY8046910.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            36823..37857
                     /gene="rhaT"
                     /locus_tag="JNY73_000802"
                     /pseudo
     CDS             36823..37857
                     /gene="rhaT"
                     /locus_tag="JNY73_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(37854..38791)
                     /gene="rhaR"
                     /locus_tag="JNY73_000803"
                     /pseudo
     CDS             complement(37854..38791)
                     /gene="rhaR"
                     /locus_tag="JNY73_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(38775..39611)
                     /gene="rhaS"
                     /locus_tag="JNY73_000804"
                     /pseudo
     CDS             complement(38775..39611)
                     /gene="rhaS"
                     /locus_tag="JNY73_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            39899..41368
                     /gene="rhaB"
                     /locus_tag="JNY73_000805"
     CDS             39899..41368
                     /gene="rhaB"
                     /locus_tag="JNY73_000805"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY8046911.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            41365..42624
                     /gene="rhaA"
                     /locus_tag="JNY73_000806"
     CDS             41365..42624
                     /gene="rhaA"
                     /locus_tag="JNY73_000806"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY8046912.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            42866..43690
                     /gene="rhaD"
                     /locus_tag="JNY73_000807"
     CDS             42866..43690
                     /gene="rhaD"
                     /locus_tag="JNY73_000807"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY8046913.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            43700..44014
                     /gene="rhaM"
                     /locus_tag="JNY73_000808"
     CDS             43700..44014
                     /gene="rhaM"
                     /locus_tag="JNY73_000808"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY8046914.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            44315..>44381
                     /locus_tag="JNY73_000809"
     CDS             44315..>44381
                     /locus_tag="JNY73_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY8046915.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT        10877 a        11757 c        11422 g        10325 t
ORIGIN      
        1 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
       61 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
      121 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
      181 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
      241 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
      301 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
      361 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
      421 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
      481 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
      541 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
      601 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
      661 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
      721 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
      781 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
      841 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
      901 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
      961 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
     1021 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
     1081 cagataagtg tgagcacaac caggccactg attgccagtg ggttgctctt tcctgcggca
     1141 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
     1201 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
     1261 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
     1321 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
     1381 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
     1441 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
     1501 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
     1561 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
     1621 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
     1681 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
     1741 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
     1801 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
     1861 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
     1921 cagtcgcgct gcggcattaa tgccagacgc gcaccgttga caccaccgcg tttgtcgcca
     1981 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
     2041 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
     2101 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
     2161 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
     2221 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
     2281 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
     2341 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
     2401 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg ggtcggcgtc
     2461 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
     2521 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
     2581 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
     2641 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
     2701 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
     2761 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
     2821 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
     2881 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
     2941 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
     3001 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
     3061 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
     3121 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
     3181 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
     3241 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
     3301 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
     3361 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
     3421 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
     3481 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
     3541 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
     3601 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
     3661 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
     3721 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
     3781 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
     3841 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
     3901 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
     3961 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
     4021 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
     4081 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
     4141 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
     4201 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
     4261 accacttccc ggcggcagat cgccacgcag tgccacgata tgacaaatac cgttattcca
     4321 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
     4381 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
     4441 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
     4501 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
     4561 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
     4621 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
     4681 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
     4741 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
     4801 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
     4861 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
     4921 caatatattt cattagaaac catagccccg aaaacatgag taccgggcat tattaaattt
     4981 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
     5041 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
     5101 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatggtctt cacgcaccgc
     5161 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
     5221 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
     5281 ttcaaagaaa tggtcgatgc tgccaccttc gcaatgagca ggcaccagcg attccacacg
     5341 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
     5401 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
     5461 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
     5521 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
     5581 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
     5641 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
     5701 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
     5761 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
     5821 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
     5881 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
     5941 cacaaattca aagccagtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
     6001 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
     6061 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
     6121 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
     6181 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
     6241 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
     6301 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc agcaactggc gatcgttatg
     6361 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
     6421 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
     6481 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
     6541 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
     6601 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
     6661 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
     6721 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
     6781 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
     6841 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
     6901 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
     6961 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
     7021 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
     7081 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
     7141 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
     7201 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
     7261 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
     7321 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
     7381 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
     7441 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
     7501 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
     7561 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
     7621 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
     7681 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
     7741 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
     7801 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
     7861 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
     7921 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
     7981 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
     8041 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
     8101 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
     8161 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
     8221 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
     8281 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
     8341 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
     8401 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
     8461 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
     8521 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
     8581 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
     8641 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
     8701 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
     8761 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt accaacataa attgtggggt
     8821 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
     8881 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
     8941 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
     9001 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
     9061 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
     9121 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
     9181 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
     9241 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
     9301 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
     9361 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
     9421 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
     9481 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
     9541 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
     9601 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
     9661 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
     9721 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
     9781 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
     9841 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
     9901 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
     9961 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    10021 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    10081 ccggtagcaa catcacgctg tttgccggtg aagaacgggt ggcacttgct gcacacgtcg
    10141 aggttcaggt catgaccaac ggtggagcgg attttcatta cgttaccgca agagcagcta
    10201 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    10261 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    10321 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    10381 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    10441 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    10501 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    10561 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    10621 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    10681 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    10741 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    10801 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    10861 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    10921 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    10981 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    11041 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    11101 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    11161 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    11221 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    11281 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    11341 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    11401 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    11461 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    11521 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    11581 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    11641 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    11701 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    11761 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    11821 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    11881 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    11941 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    12001 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    12061 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    12121 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    12181 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    12241 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa
    12301 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    12361 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    12421 atcctctcca gtccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    12481 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    12541 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    12601 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    12661 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    12721 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgta
    12781 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    12841 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    12901 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    12961 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    13021 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    13081 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    13141 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    13201 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    13261 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    13321 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    13381 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    13441 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    13501 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    13561 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    13621 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    13681 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    13741 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    13801 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    13861 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    13921 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    13981 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    14041 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    14101 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    14161 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    14221 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    14281 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    14341 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    14401 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    14461 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    14521 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    14581 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    14641 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    14701 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    14761 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    14821 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    14881 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    14941 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    15001 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    15061 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    15121 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    15181 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    15241 ttgaagcact gctggcagtc gctgatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    15301 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    15361 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    15421 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    15481 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    15541 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    15601 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    15661 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    15721 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    15781 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    15841 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    15901 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    15961 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    16021 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    16081 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    16141 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    16201 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    16261 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    16321 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    16381 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    16441 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    16501 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    16561 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    16621 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    16681 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    16741 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    16801 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    16861 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    16921 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    16981 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    17041 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    17101 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    17161 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    17221 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    17281 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    17341 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    17401 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    17461 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    17521 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    17581 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    17641 tttcgctgca tagcctgtgg ggctggctgt ttctgctggc ggcaccatta ctggtgaagc
    17701 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    17761 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    17821 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    17881 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    17941 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    18001 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    18061 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    18121 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    18181 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    18241 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    18301 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    18361 cgacaatacc gggattattc tttcagaaga tccgctagat attgaatgat aagaaaggca
    18421 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    18481 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    18541 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    18601 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    18661 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttatcaaaca
    18721 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    18781 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    18841 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    18901 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    18961 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    19021 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    19081 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    19141 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    19201 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    19261 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    19321 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    19381 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    19441 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    19501 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    19561 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    19621 atggtgatta ccgctattct gatggggctt atcctggcgt taacggacga tggcaacggt
    19681 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    19741 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    19801 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    19861 ttcctggtgc cgcttttcgg ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    19921 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    19981 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    20041 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    20101 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    20161 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    20221 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    20281 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    20341 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    20401 tatatcagca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    20461 tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga attgctgttt
    20521 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    20581 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    20641 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    20701 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    20761 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    20821 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    20881 gaaaatggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    20941 gaaggtgcgg tgtttatggc aggcacatcc attcagtggc tgcgcgatga gatgaagttg
    21001 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    21061 tatgtggttc cggcatttac cgggctgagt gcgccgtact gggacccgta tgcgcgcggg
    21121 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    21181 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    21241 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    21301 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    21361 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    21421 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    21481 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    21541 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    21601 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    21661 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    21721 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    21781 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    21841 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    21901 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    21961 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    22021 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    22081 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    22141 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    22201 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    22261 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    22321 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    22381 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    22441 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    22501 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    22561 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    22621 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    22681 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    22741 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    22801 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    22861 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    22921 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    22981 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    23041 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    23101 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    23161 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    23221 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    23281 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    23341 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    23401 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    23461 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    23521 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    23581 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    23641 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    23701 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    23761 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    23821 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    23881 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    23941 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    24001 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    24061 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    24121 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    24181 taatcttata ggccacgctg gccccttcat ctgcagagaa tgcagcggcg gcaagctctt
    24241 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    24301 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    24361 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    24421 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    24481 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    24541 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggtcaatta
    24601 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    24661 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    24721 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    24781 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    24841 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    24901 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    24961 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    25021 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    25081 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    25141 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    25201 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    25261 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    25321 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    25381 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    25441 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    25501 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    25561 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    25621 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    25681 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    25741 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    25801 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    25861 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    25921 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    25981 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    26041 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    26101 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    26161 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    26221 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    26281 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttatg
    26341 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    26401 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    26461 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    26521 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    26581 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    26641 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    26701 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    26761 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    26821 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    26881 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    26941 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    27001 tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    27061 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    27121 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    27181 gacatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    27241 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    27301 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    27361 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    27421 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    27481 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    27541 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    27601 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    27661 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    27721 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    27781 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    27841 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    27901 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    27961 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    28021 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    28081 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    28141 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    28201 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    28261 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    28321 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    28381 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    28441 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    28501 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    28561 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    28621 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    28681 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    28741 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    28801 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    28861 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    28921 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    28981 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    29041 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    29101 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    29161 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    29221 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    29281 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    29341 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    29401 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    29461 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    29521 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    29581 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    29641 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    29701 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    29761 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    29821 caaaatgaaa tctgttgccg gaaatcttct tgcacatcga agtgatccaa cgaatgtgca
    29881 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    29941 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    30001 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    30061 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    30121 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    30181 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    30241 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    30301 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    30361 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    30421 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    30481 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    30541 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    30601 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    30661 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    30721 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    30781 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    30841 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    30901 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    30961 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    31021 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    31081 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    31141 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    31201 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    31261 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    31321 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    31381 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    31441 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    31501 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    31561 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    31621 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    31681 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    31741 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    31801 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    31861 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    31921 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    31981 tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc gcactggagc
    32041 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    32101 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    32161 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    32221 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    32281 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    32341 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    32401 tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg ttacccaagc
    32461 tcgattcacg ccagatgacc aagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    32521 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    32581 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    32641 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    32701 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    32761 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    32821 gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    32881 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    32941 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    33001 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    33061 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    33121 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    33181 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    33241 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    33301 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    33361 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    33421 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    33481 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    33541 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    33601 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    33661 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    33721 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    33781 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    33841 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    33901 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    33961 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    34021 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    34081 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    34141 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    34201 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    34261 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    34321 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    34381 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    34441 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    34501 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    34561 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    34621 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc aggtttattt
    34681 caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg accagataga
    34741 ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca gcgaagttgc
    34801 tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag gtgtcgctac
    34861 cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac cgccaatcaa
    34921 tttatcggcg ataatcaacg gaataccggt cacgataatt attgccacac ccaacaggat
    34981 ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca gcgcgaaggc
    35041 aaagaatgga atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt cagggtcaag
    35101 attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa catgcggttc
    35161 aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc cggactccaa
    35221 cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca tgatggaagc
    35281 gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg aaagtccggc
    35341 aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg ccgcaaccac
    35401 ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta ccgttcccgt
    35461 cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa tgggcaccgt
    35521 accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg gcgagaaggt
    35581 gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga ttttctcaat
    35641 cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtaat
    35701 tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta atcaataaaa
    35761 gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt ataggatcat tgtttttatt
    35821 ttggaattga tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgatat
    35881 gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcaaaacgtg
    35941 ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtcc ggacggcggt
    36001 tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg
    36061 aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta gaaaccaccg
    36121 ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg gaagctgccg
    36181 ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg atagccgctt
    36241 tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag
    36301 cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt
    36361 tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt
    36421 tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat
    36481 cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat
    36541 tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt ggtttattat
    36601 agttaattaa atgatattga aaatgattat caatgccgca cttttcgtaa gggtatggtt
    36661 ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg attgccagta
    36721 aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt acactcatct
    36781 cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa cgcgattacg
    36841 atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta cgctccgttc
    36901 aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat tgtttcgtgg
    36961 attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc gtattacagc
    37021 tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg ggggatcggt
    37081 aatatcaact atggcctgac catgcgttac ctcggcatgt cgatgggaat tggcatcgcc
    37141 attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg caatttcgat
    37201 gtgctgatta acaccgaagg cggacgcatg acgttactcg gcgttctggt ggcgctgatt
    37261 ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg cattaaagcc
    37321 gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat tttctctgcc
    37381 gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc cgcacttggc
    37441 gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg cggcgcgatc
    37501 attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc gctaaaagcc
    37561 gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc actgggcggg
    37621 ttgatgtggt atctgcaatt ctttttctat gcctagggcc acgcccgcat tccggcgcag
    37681 tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg cggtatcgtc
    37741 gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt gttgagcctc
    37801 ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc gaattaatct
    37861 ttctgcgaat tgagatgcgc cactggctgg gcgtcatccc ggtttcccgg gtaaacacca
    37921 ccgaaaaata gttactatct tcaaagccac attcggtcga aatatcactg attaacaggc
    37981 ggctatgctg gagaagatat tgcgcatgac acactctgac ctgccgcaga tattgattga
    38041 tggtcattcc agtctgctgg cgaaattgct gacgcaaaac gcgctcactg cacgatgcct
    38101 catcacaaaa tttatccagc gcaaagggac ttttcaggct agccgccagc cgggtaatca
    38161 gcttatccag caacgtttcg ctggatgttg gcggcaacga atcactggtg taacgatggc
    38221 gattcagcaa catcaccaac tgcccgaaca gcaactcagc catttcgtta gcaaacggca
    38281 catgctgact actttcatgc tcaagctgac cgataacctg ccgcgcctgc gccatcccca
    38341 cgctacctaa gcgccagtgt ggttgccctg cgctggcgct aaatcccgga atcgctccct
    38401 gccagtcaag attcagcttc agccgctccg ggcaataaat aatattctgc aaaaccagat
    38461 cgttaacgga agcgtaggag tgtttatcgt cagcatgaat ataaaagaga tcgccacggg
    38521 taatgcgata aggacgatcg ttgagtacat gcagaccatt accgcgccag acaatcacca
    38581 gctcacaaaa atcatgtgta tgttcggcaa agacatcttg cggataacgg tcagccacag
    38641 cgactgcctg ctggtcgctg gcaaaaaaat catctttgag aagttttaac tgatgcgcca
    38701 ccgtggctac ctcggccaga gaacgaagtt gattattcgc aatatggcgt acaaatacgt
    38761 tgagaagatt cgcgttattg cagaaagcca tcccgtccct ggcgaatatc acgcggtgac
    38821 cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga
    38881 taggcgatgt cagtgacgct ggcctcgctg tggcgtagca gatgtcaggc tttcatcagt
    38941 cgcaggcggt tcaggtatcg ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt
    39001 agcgtacgca gtgaaagaga aaattgatcc gccacggcat cccaattcac ctcatcggca
    39061 aaatggtcct ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc
    39121 tcctgcaaac tgcttttacg cagcaagagc agtaattgca taaacaagat ctcgcgactg
    39181 gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc
    39241 acctgctgca atacgctgtg gttaacgcgc cagtgagacg gatactgccc atccagctct
    39301 tgtggcagca actgattcag cccggcgaga aactgaaatc gatccggcga gcgatacagc
    39361 acattggtca gacacagatt atcggtatgt tcatacagat gccgatcatg atcgcgtacg
    39421 aaacagaccg tgccaccggt gatggtatag ggctgcccat taaacacatg aatacccgtg
    39481 ccatgttcga caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc
    39541 gggagccggg gttctatcgc cacggacgcg ttacaagacg gaaaaaaatc cacactatgt
    39601 aatacggtca tactggcctc ctgatgtcgt caacacggcg aaatagtaat cacgaggtca
    39661 ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac
    39721 agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc acaattcagc aaattgtgaa
    39781 catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa
    39841 ggtcgcgaat tcaggcgctt tttagactgg tcgtaatgaa attcagcagg atcacattat
    39901 gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc
    39961 gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg
    40021 gctgcatagc caaaacggtt atgtcacctg ggatgtggat agcctggaaa gtgccattcg
    40081 ccttggatta aacaaggtgt gcgaggaagg gattcgtatc gatagcattg ggattgatac
    40141 ctggggcgtg gactttgtgc tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc
    40201 ttatcgcgat agccgcacca atggcctgat gccgcaggca caacagcaac tcggcaaacg
    40261 cgatatttat cagcgtagcg gcatccagtt tctacccttc aatacgcttt atcagttgcg
    40321 tgcgctgacg gagcaacaac ctgaacttat tccacacatt gctcacgctc tgctgatgcc
    40381 ggattacttc agttatcgcc tgaccggcaa gatgaactgg gaatatacca atgccaccac
    40441 cacacaactg gtcaatatca atagcgacga ctgggacgag tcgctactgg cgtggagcgg
    40501 ggccaacaaa gcctggtttg gtcgcccgac gcatccgggt aatgtcattg ggcactggat
    40561 ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc agccatgata ccgccagcgc
    40621 ggttatcgcc tcgccgttaa acggttcacg cgccgcttat ctctcttctg gcacctggtc
    40681 attgatgggc ttcgaaagcc agacgccatt taccaatgac acggcgctgg cagccaacat
    40741 caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg
    40801 gctgcttcag cgagtgcttc aggaacggca aatcaacgat ctcccggcgc ttatcgccgc
    40861 gacacaggca cttccggcct gccgcttcat catcaatccc aatgacgatc gctttattaa
    40921 tcctgacgag atgtgcagcg aaattcaggc tgcgtgtcgg gaaacggcgc aaccgatccc
    40981 gggaagtgat gctgaactgg cgcgctgtat tttcgacagt ctggcgctgc tgtatgccga
    41041 tgtgttgcat gagctggcgc agctgcgcgg tgaagatttc tcgcaactgc atattgtcgg
    41101 cggcggctgc cagaacacgc tgctcaacca gctatgcgcc gatgcctgcg gtattcgggt
    41161 gatcgccggg cctgttgaag cctcaacgct cggcaatatc ggcatcgagt taattacgct
    41221 ggatgaactc aacaatgtgg atgatttccg tcaggtcgtc agcaccaccg cgaatctgac
    41281 cacctttacc cctaatcctg acagtgaaat tgcccactat gtggcgcaga ttcactctac
    41341 acgacagaca aaggagcttt gcgcatgacc actcaactgg aacaggcctg ggagctggcg
    41401 aaacagcgtt tcgcggcggt ggggattgat gtcgaggagg cgctgcgcca acttgatcgt
    41461 ttacccgttt caatgcactg ctggcagggc gatgatgttt ccggttttga aaacccggaa
    41521 ggttcgctga ccggggggat tcaggccaca ggcaattatc cgggcaaagc gcgtaatgcc
    41581 agtgagctac gtgccgatct ggaacaggct atgcggctga ttccggggcc gaaacggctt
    41641 aatttacatg ccatctatct ggaatcagat acgccagtct cgcgcgacca gatcaaacca
    41701 gagcacttca aaaactgggt tgaatgggcg aaagccaatc agctcggtct ggattttaac
    41761 ccctcctgct tttcgcatcc gctaagcgcc gatggcttta cgctctcgca tcccgacgac
    41821 agcattcgcc agttctggat tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt
    41881 ggcgagcaac tcggcacacc atcggtgatg aacatctgga tcccggatgg tatgaaagat
    41941 atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc
    42001 agcgagaagc taaaccctgc gcaccatatc gacgccgttg agagcaaatt gtttggcatt
    42061 ggcgcagaga gctacacggt tggctccaat gagttttaca tggggtatgc caccagccgc
    42121 cagactgcgc tgtgcctgga cgccgggcac ttccacccga ctgaagtgat ttccgacaag
    42181 atttccgccg ccatgctgta tgtgccgcag ttgctgctgc acgtcagccg tccggttcgc
    42241 tgggacagcg atcacgtagt gctgctggat gatgaaaccc aggcaattgc cagtgagatt
    42301 gtgcgtcacg atctgtttga ccgggtgcat atcggccttg acttcttcga tgcctctatc
    42361 aaccgcattg ccgcgtgggt cattggtaca cgcaatatga aaaaagccct gctgcgtgcg
    42421 ttgctggaac ctaccgctga gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt
    42481 ctggcactgc tggaagagca gaaatcgttg ccgtggcagg cggtctggga aatgtattgc
    42541 caacgtcacg atacgccagc aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa
    42601 gcgattttga gccagcgtgg gtaaacactg ccggatgcgg cacgagcgcc ttatccggcc
    42661 tacgggtcga atgtccatgc gggctgtcac cctcaccccg gccctctccc tgaaagggcg
    42721 agggagaaaa acatagcctc gtgcggcaac ggagctgaac accgctccgg acagtcccct
    42781 cgcccctacg gggagagggt tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc
    42841 aacctaattt acaggaacac agaacatgca aaacattacc cagtcctggt ttgtccaggg
    42901 aatgatcaaa gccaccaccg atgcctggct gaaaggctgg gacgagcgca acggcggcaa
    42961 cctgacgctg cgcctggatg acgccgatat cgcgctttat cacgacaact tccacccaca
    43021 accgcgctat attccgctca gccaacccat gcctttactg gcaaatacgc cgtttattgt
    43081 caccggctcc ggcaaattct tccgtaacgt ccagctcgat cctgcagcta acttaggcgt
    43141 cgtaaaagtc gacagcgatg gcgcgggcta ccatattctc tgggggttaa ccaacgaagc
    43201 cgttccaact tctgaacttc cggctcactt tctctcccac tgcgagcgca ttaaagccac
    43261 caacggtaaa gatcgggtga tcatgcactg ccacgccacc aacctgatcg ccctcaccta
    43321 tgtactggaa aacgacaccg cggtctttac tcgccaactg tgggaaggca gcaccgaatg
    43381 tctggcagta ttcccggatg gcgttggcat tctgccgtgg atggtgcccg gcacggacga
    43441 aatcggccag gcgaccgcac aagagatgca aaaacattcg ctggtgttgt ggcccttcca
    43501 cggtgtgttc ggttccggac cgacgctgga tgaagccttt ggtttaatcg acaccgcaga
    43561 aaaatcagca caagtattag tgaaggttta ttcgatgggc ggcatgaaac agaccatcag
    43621 ccgtgaagag ttgatagcgc tcggcaagcg cttcggcgtt acaccactag ccagtgcgct
    43681 ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc atgcaggtaa accccgacgc
    43741 ccacgaagag tatcagcgtc ggcataatcc catctggccg gaactggaag cagtgttgaa
    43801 atctcacggt gtgcataact acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc
    43861 catggtagag attgaatctg aagaacggtg gaatgcggta gccagtactg atgtttgcca
    43921 acgttggtgg aaatatatga ccgatgttat gcccgctaac ccagataaca gcccggtgag
    43981 tagcgagctg caagaagtgt tttacctgcc gtaattgccc aatgtgccgg agatgcctcc
    44041 ggcacaatat tttaaatttc gatttgaaaa attaaacata ttacatctcc cggtaagtta
    44101 tatttccctg atacattgtg agtaaatcac aaaaataatg aataacccat taatgattca
    44161 tgtggtttat ttaaataacc attatgtgca ttactccgca aatctgacct ttcactctgt
    44221 ctcaaattgt cattgtttac cgacatatcg cctctcatca cttaatcgct ccaccattac
    44281 aattgattaa aatatatttt atggagtccg attaatggca gctcttactg caagctgtat
    44341 tgacctgaat attcagggca atggcgctta ttccgttctg a
//
LOCUS       DADLZM010000014        44105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLZM010000014 DADLZM010000000
VERSION     DADLZM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.014"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="JNY73_000810"
     CDS             complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="JNY73_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HAY8046916.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVAFAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="JNY73_000811"
     CDS             complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="JNY73_000811"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY8046917.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5526..5930)
                     /locus_tag="JNY73_000812"
     CDS             complement(5526..5930)
                     /locus_tag="JNY73_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY8046918.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="JNY73_000813"
     CDS             complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="JNY73_000813"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY8046919.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIV"
     gene            complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="JNY73_000814"
     CDS             complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="JNY73_000814"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY8046920.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="JNY73_000815"
     CDS             complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="JNY73_000815"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY8046921.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9966..10743
                     /gene="glcC"
                     /locus_tag="JNY73_000816"
                     /pseudo
     CDS             9966..10743
                     /gene="glcC"
                     /locus_tag="JNY73_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            complement(10963..12009)
                     /locus_tag="JNY73_000817"
     CDS             complement(10963..12009)
                     /locus_tag="JNY73_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8046922.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKICGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKRMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEM"
     gene            12413..14101
                     /locus_tag="JNY73_000818"
                     /pseudo
     CDS             12413..14101
                     /locus_tag="JNY73_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14116..15012
                     /locus_tag="JNY73_000819"
     CDS             14116..15012
                     /locus_tag="JNY73_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY8046923.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            15045..15293
                     /locus_tag="JNY73_000820"
     CDS             15045..15293
                     /locus_tag="JNY73_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8046924.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15293..16465
                     /locus_tag="JNY73_000821"
     CDS             15293..16465
                     /locus_tag="JNY73_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY8046925.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16501..17580)
                     /gene="lptG"
                     /locus_tag="JNY73_000822"
     CDS             complement(16501..17580)
                     /gene="lptG"
                     /locus_tag="JNY73_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY8046926.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17577..18647)
                     /gene="lptF"
                     /locus_tag="JNY73_000823"
     CDS             complement(17577..18647)
                     /gene="lptF"
                     /locus_tag="JNY73_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY8046927.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18678..19238)
                     /locus_tag="JNY73_000824"
     CDS             complement(18678..19238)
                     /locus_tag="JNY73_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY8046928.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19250..20074)
                     /locus_tag="JNY73_000825"
     CDS             complement(19250..20074)
                     /locus_tag="JNY73_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046929.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20074..21423)
                     /locus_tag="JNY73_000826"
     CDS             complement(20074..21423)
                     /locus_tag="JNY73_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY8046930.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNIVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21469..22227)
                     /locus_tag="JNY73_000827"
     CDS             complement(21469..22227)
                     /locus_tag="JNY73_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046931.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22259..22972)
                     /locus_tag="JNY73_000828"
     CDS             complement(22259..22972)
                     /locus_tag="JNY73_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY8046932.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23146..23838
                     /locus_tag="JNY73_000829"
     CDS             23146..23838
                     /locus_tag="JNY73_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY8046933.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23887..25386)
                     /gene="pitB"
                     /locus_tag="JNY73_000830"
     CDS             complement(23887..25386)
                     /gene="pitB"
                     /locus_tag="JNY73_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY8046934.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGVNDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25678..27537)
                     /gene="gss"
                     /locus_tag="JNY73_000831"
     CDS             complement(25678..27537)
                     /gene="gss"
                     /locus_tag="JNY73_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY8046935.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27742..28608
                     /gene="yghU"
                     /locus_tag="JNY73_000832"
     CDS             27742..28608
                     /gene="yghU"
                     /locus_tag="JNY73_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY8046936.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGNEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28731..28979)
                     /gene="hybG"
                     /locus_tag="JNY73_000833"
     CDS             complement(28731..28979)
                     /gene="hybG"
                     /locus_tag="JNY73_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY8046937.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29113..29454)
                     /gene="hypA"
                     /locus_tag="JNY73_000834"
     CDS             complement(29113..29454)
                     /gene="hypA"
                     /locus_tag="JNY73_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY8046938.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29447..29935)
                     /gene="hybE"
                     /locus_tag="JNY73_000835"
     CDS             complement(29447..29935)
                     /gene="hybE"
                     /locus_tag="JNY73_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY8046939.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29928..30422)
                     /locus_tag="JNY73_000836"
     CDS             complement(29928..30422)
                     /locus_tag="JNY73_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY8046940.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30422..32125)
                     /gene="hybC"
                     /locus_tag="JNY73_000837"
     CDS             complement(30422..32125)
                     /gene="hybC"
                     /locus_tag="JNY73_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY8046941.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32122..33300)
                     /gene="hybB"
                     /locus_tag="JNY73_000838"
     CDS             complement(32122..33300)
                     /gene="hybB"
                     /locus_tag="JNY73_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY8046942.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33290..34276)
                     /gene="hybA"
                     /locus_tag="JNY73_000839"
     CDS             complement(33290..34276)
                     /gene="hybA"
                     /locus_tag="JNY73_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY8046943.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVMVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34279..35397)
                     /gene="hybO"
                     /locus_tag="JNY73_000840"
     CDS             complement(34279..35397)
                     /gene="hybO"
                     /locus_tag="JNY73_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY8046944.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPYNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35586..35873)
                     /locus_tag="JNY73_000841"
     CDS             complement(35586..35873)
                     /locus_tag="JNY73_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY8046945.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35992..36918)
                     /locus_tag="JNY73_000842"
     CDS             complement(35992..36918)
                     /locus_tag="JNY73_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY8046946.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            37036..38076
                     /gene="gpr"
                     /locus_tag="JNY73_000843"
     CDS             37036..38076
                     /gene="gpr"
                     /locus_tag="JNY73_000843"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY8046947.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38116..38610)
                     /locus_tag="JNY73_000844"
     CDS             complement(38116..38610)
                     /locus_tag="JNY73_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY8046948.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38801..39685
                     /gene="yghA"
                     /locus_tag="JNY73_000845"
     CDS             38801..39685
                     /gene="yghA"
                     /locus_tag="JNY73_000845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY8046949.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     ARLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39957..40382)
                     /gene="exbD"
                     /locus_tag="JNY73_000846"
     CDS             complement(39957..40382)
                     /gene="exbD"
                     /locus_tag="JNY73_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY8046950.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40389..41123)
                     /locus_tag="JNY73_000847"
     CDS             complement(40389..41123)
                     /locus_tag="JNY73_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY8046951.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41375..42562
                     /gene="metC"
                     /locus_tag="JNY73_000848"
     CDS             41375..42562
                     /gene="metC"
                     /locus_tag="JNY73_000848"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY8046952.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVVRVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42702..43361
                     /locus_tag="JNY73_000849"
     CDS             42702..43361
                     /locus_tag="JNY73_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8046953.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43401..>44105)
                     /locus_tag="JNY73_000850"
     CDS             complement(43401..>44105)
                     /locus_tag="JNY73_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8046954.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10941 a        11762 c        10842 g        10560 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagcttgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taaattaatg
      541 attttttcat ttcttcgcgc ctaataaaag tattcaaatt cagatgattt ttctgctcaa
      601 cagacacaaa caggcaataa tactctccaa agcgaacaat atttggcatt acgatttaat
      661 gcttcataat caccaaatta tttaaccata aattttctta tttctaacaa aatgattggt
      721 gtgttgttgg agttaaaata catacataat attaacatct gccaacattt cactttccgc
      781 aagacggatg atttcgaatc ctcatccacg ttaccctatc ccaaatatct ttattaatag
      841 gcagtaacga acactgttat agcaagatta acttattaac ctaacggcat cttttatgta
      901 acaagtggag ataaattgct taaaattgat cctcgacaaa tcttaaaagt tctcattatt
      961 ctgcaaaatc agctttgcga ataatcttat gtttttggga gttttgggaa ttttgatcta
     1021 ctcattgttc tagtgaaaaa tataaataag agaaaaaacg ccattcgtca tgaaagcaac
     1081 agatgaagca atgtaataac atactaaaat tgatctcatg aaagtgggta ctttataacc
     1141 agcacagaag ccaagttgta taaatattaa acaattcgca tcgtgaaacg gagactacgt
     1201 ataaagaagc caatcgtaaa aacggttccc tggcgagata aacgacagac aaatggaaag
     1261 ccgggtgata tttaccttca tccggcttta ttgatttacg agactaacat cccggtaaac
     1321 acatacgcct gcagcagggt gataacgccg ataacgctgg caaaaatcag actgtgcttc
     1381 acggtgtagc ggaacagttc agattctcgg cccaccatgc ccgtcgcggc gcaggccacg
     1441 gcgatagatt gcggggagat catcttgcca gttacgccgc cgctggtatt tgctgccacc
     1501 agcagagtgt cagagacgtt gatttgctgc gccgtggtcg attgcagtga accaaacagg
     1561 gcgtttgagg aagtgtccga cccggtaagg aatacgccca gccagccgag aaacggtgag
     1621 aaaaacggga acatcacgcc tgtacctgcc agtaccagcg ccagcgtggt ggacatgccg
     1681 gaatagttgg tgacgaaggc gaatgccagc accatgccaa tcgacaatat cggccacttc
     1741 agactaatta gcgtttcggc aaagacgcca atccctttct tgatgcccac gccgaggatg
     1801 aagatagaga taatcgccgc aataaaaata gcggtgccgc cagccgagag aggttcgaat
     1861 ttaaacaccg catccattgg cgttggttgg gcgacaatgg gtgccgcttt caacacttgt
     1921 tgatgcaaat gagggatctg gaaattaatc accagtgaat aaaacgcgcc gcccggagca
     1981 aataacgctt taaacggctt catggtccag atggtgacca gcaccgttaa gattaaaaac
     2041 ggtgaccacg cacgaatgat ttgccccaga ctatattctg aaggcacggg accgccagaa
     2101 gatggcttat ttaccaccat cgcacctgcg gattgcccca tgctgattgc cgtttcggta
     2161 tttttcggcc gccagacttt aaggaataaa gcgagtgaga cgatactcac cagcgccgaa
     2221 gtaatatccg gcagttccgg accaatatag ttagaggtaa agaactgagt gacagcgaag
     2281 cttcccccag caaccagcgc cgctggccac gtctctttca cccctttcca gccgtccatc
     2341 attgctacca gccagaacgg cacaagaacc gacaggaacg gtaactgacg tcccgccatt
     2401 gcgccaatgt ggaacggatc gattcccgtt acctgaccgg cgaccagaat cggcacgccc
     2461 aacgcaccaa acgccaccgg cgcagtattg gcaatcagac acagccccgc cgcgtataac
     2521 ggtttgaagc ccaggcccac cagcagcgca ccggtaatcg ccaccggcgc accaaagcca
     2581 gccgctcctt ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga
     2641 tcgtcggtga tggagataac cgagctgcgg ataatatcga actgcccgct ggcaacggtt
     2701 aatttataca ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata
     2761 aagccatagc ccgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca
     2821 atcaggatag ataatataag ggttattgct ccagcgacat gtcctttcag acgtaatacc
     2881 gcgaatgcaa cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt
     2941 cctcccatcg gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct
     3001 ttatatgctt gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata
     3061 ggcgttatac tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa
     3121 gtatatcggg taagggtttc tgttccgcac acgcaggcgc agagtatcgt taagatgtcc
     3181 atattgttgt tttaggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg
     3241 ataatcgctc cggttatttc cgggataaat gtactaccgc agttactatc atagccccga
     3301 caataaaact tgccggggct tttttgacgc tattaatgac tttctttttc gcgtaaacgc
     3361 caggcgtgta ataacggttc ggtataaccg tttggctgtt tcacgccgag gaagattaaa
     3421 tcgctggcag ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg acgataagcc
     3481 ggatcgccag cattttgctg atcaaccact ttcgccatat tctccagcga cgcctgcacc
     3541 tgttctttgg tcagaatacc gtgacgtaac cagttggcga tatgctggct ggagatacgc
     3601 agcgttgcgc ggtcttccat cagcgccacg ttgtgaatat ccggcacttt tgaacaacca
     3661 atcccctgct ccacccaacg caccacgtac cccagaatcc cctgcacgtt gttatccagc
     3721 tcttgttgga tctcttgcgc cgaccagtta gcgttttcag ccaccggaat agtcagcaga
     3781 tcgtccagca gcggttcaaa ttcagcattg aactcggtct gggcaatgtt ggcttgcacg
     3841 ctctgtacgt tggtttggtg gtagtgcagc gcatggagcg tagcagcggt tggtgacgga
     3901 acccaggctg tgtttgcccc ggcacgcagt tggtcgccct tctggctgta catgtctgcc
     3961 atcaggtccg gcattgccca catgccttta ccgatttgcg ctttaccgcg cagcccacag
     4021 aacagaccgg aaagcacgtt attacgttcg taggctttga tccacggcgt cgatttcatc
     4081 tggtttttac gcagcatcgg gccagcttcc atcaccgaat gcatttcatc gccggtacgg
     4141 tcgaggaaac cggtattgat gaacgccacg cggttgcgcg cctgagcgat acagctccgc
     4201 aagttcagcg aggtccggcg ttcttcatcc ataatgccca ttttcagggt attcggtacc
     4261 ataccgagca ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac ttcctgcgga
     4321 ccgtgcattt tcggtttcac aatatagacg ctgccagtgc gcgagttttt ctgcactttt
     4381 aaatcataga gggcaatcgc gccagtcatg acgccatcaa gaataccttc cgggatttca
     4441 ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt gcggataaac
     4501 agcaacgagc gtccgtgcag agaaatttca gagccatcgg cggcggtgta ctgacgatcg
     4561 tcattcagtt tacgtacgat ttgccgaccg tttttctcca ttttctcttg cagagtcccc
     4621 tgcatcaggc ccagcagatt gcggtacagc aggattttat cttccgcatc aaccgccgcg
     4681 accgaatctt cgcagtcgag aatggtactg atagcagctt cgacgataac atcgttgatg
     4741 tgcgccggat cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc aatatgcagg
     4801 ccgttatttt tcagcaaaat gcaggtcggc gcagcggcat cgccacggta accgacaaac
     4861 tgtgctggag tacgtaacgt ggtttcttta ccatttttca actggatgcg taattgttta
     4921 tcgaccacct taaacgccac cacatcctga tagctgccgt tttccagcgg tagtgattca
     4981 tcgaggaaac gtcgaaccca ggcgataacc tgctcaccgc gctgcggatc gtagccactg
     5041 accatcgccc cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta cagtgagccc
     5101 cagcgagcgt tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac caccagctgt
     5161 ggccccgcct gactggtgat ttcgctgtca atgcccgtgg tttccaccgt cacgcgctcc
     5221 ggttgcggca ccaggtagcc cagttcacaa aggaaagatt tataggccgc tttatctttt
     5281 accggccccg gattgctgcg atgccactca tcaagcgccg cctgaatgcg atcgcgttct
     5341 gccagcaact gacgattttc tggtgccaga tcgtgaacga tctcatcaac attgtgccag
     5401 aatgcagcag cgtccagttc cacgcccggc aaaacttctc catccacaaa acgtttaaaa
     5461 ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac tcattgtttc tctcctcgtt
     5521 ttcgcttatt tcgccaacac cgctgctgcc gctttcgcga cctgcgcatc ctgtgctccg
     5581 gttaaaccag aaacgcccac ggcaccaata atttgcccat caacaacaac cggtacgccg
     5641 ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt gttcaccatc
     5701 tcttcatagc ccttagtttc acgacgcccc agcgcggcgg tacgcgcttt ctcctgggag
     5761 atgtaagccg caatcggcgc gcaatcgtcc atgcgactta acgccagcag atgaccgccg
     5821 tcatcggcaa cagcaatgga aacagaccag ttatttttct gcgcctcttc ctgacctgcg
     5881 gcaataattg cactcgccat ttgctggcta agaatgactt tagttttcat ttttttattc
     5941 cttttcaagg gcttgttata caatttcaat ccagtgacgc acagaggtac gaccggcgct
     6001 cgccagatgc gtctggcaac caatgttggc ggtgacgatc atttccggtt tgccgctttc
     6061 cagcgcattc attttgttat cccgcagctg gcgtgccaga tcgggatgcg ttaacgcata
     6121 tgttcccgct gaaccgcagc acagatggct gtcgggaacg tccgttaagg taaatccaag
     6181 acgaagtaac acgttttcca cttcgccgtt cagcttttgc gcatgttgta gggtacacgg
     6241 gcagtggaag gccagttttt tatcgccgcg aattgccagt ttttccagcg gttcctcgcg
     6301 cagaagttcg actaaatcga ccgccagttc actgacctga cgcgctttat cggcatataa
     6361 cgcatcgttt ttcagcatct gcccatactc tttgacgaac gcgccgcaac cgctggcggt
     6421 ttgcaaaatt gcctcggcac ctgcttcaat cgcgggccac caggcatcaa tattattgcg
     6481 cgcccgtgcc aaccctttct cctgcgcatt aagatgatag tccaccgcgc cacaacagcc
     6541 tgcttcgtta gctgacatga cgctgatccc cagacgatcc agcactcgcg cagttgccgc
     6601 gttggtgttg ggcgaaagcg taggctgggc gcagccttcc agcattaaaa cccgacgctt
     6661 atggcgcagc ggcggacgcg gtttagcttt caccgtttca gcaggcagtt ttgctctgac
     6721 ctgttccggt aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac ggaagaccgc
     6781 cggacgcggc actacctggc gcaatccttc gcgcagcatt cgctccggca gtgggcgttt
     6841 cactttctgc tcgacaatat cacgcccgat atccagcaaa ttgtgatagc gcacaccaga
     6901 aggacaggtg gtttcacaat tgcggcaagt gaggcagcga tcgagatgct cctgtgtttt
     6961 aagcgtgact tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc cgcgcggccc
     7021 gtccagttca tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa atccgcagtg
     7081 aacacaggcg cgcaggatgc tgtcggcttc cagcgcgcgc gcgttctgcc gcatctcttc
     7141 ggttaatcgg gtttgcatag cctgctcctc aaagttccgc gtacatgcga ccggggttaa
     7201 acacgccgca agggtcgagc tgctgtttaa gctgctggtg atagcggaat aaaggagccg
     7261 atagcggggc aaagccacca tctccggcac taaagcgggt cgcatgaccg ccagcgttgc
     7321 gggcgatgcg atggatttga ttgtcatcgg ctgtcgattt cagccagcgt aacgccccgc
     7381 cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg cgcatcgctg ggtaatgaaa
     7441 tgcgccataa ggtacctggt aacgagaaga acggcagttg ttgttcacgc aattgctgcc
     7501 agaactgacc cgcaacctct tcgccaccca gcagttcacg cgctgctttt accgatcctt
     7561 cgccgccctc aaggcggatc cacaacgcat tgtcgaagta acataagcca ctaatgggta
     7621 atggctggag ttgccactcg gcgatttcac tcatggcttc ttgcaggctg atttcccgac
     7681 gcaggctcag agaggcacgc ggtcgcggta acactttcat tgagatttca gtgagcacgc
     7741 caagacaacc gtagcttccg gccattaacc gtgagagatc gtatccggca acgtttttca
     7801 tcacttcgcc accaaaacgc agatgttttc cagtgccggt aatgatgcgc gtgccgagga
     7861 caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc cgccagcccg caggcgacca
     7921 tcccgcccca ggtggcttct tcaccataat gcggcggctc acagggaagc atttgcccgg
     7981 cgctctccag cgccgcttca atcgccacca gcggcgttcc ggcacgagcg gttatcacca
     8041 gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg cacatccagc gtttgcccgg
     8101 tgactgggcg acctaaaaag gctttgctat tgctgccctg aatcaccagc ggcgttttat
     8161 cgctaattgc ctgattcacc tgctccagca gcgcctgact gtaatcacac tcgcgtagca
     8221 tcaaaagcgc tccagttcag gaaaaggtaa atgaccgtga tgcacatgca tggcaccaaa
     8281 ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg ttcagcaaac catcggggtc
     8341 aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg ctgttgaact gggcgcacat
     8401 ttgattgatt ttttctcgcc cgatgccatg ttccccactg atgctgccgc caacttcaac
     8461 gcagagttcg aggatcttcc cgcccagctc ttccgcgcgg gcaaattcac caggttcgtt
     8521 ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg gcatgaaaga cgttggcaac
     8581 acgtaaatca tattgctgcg ataaacgggc aatgccttcc agtacgccag gcagggcgcg
     8641 acgcgggatg gtgccatcca tgcagtagta atccggggag atacgtccta ccgccgggaa
     8701 cgcatttttg cgaccggccc agaaacgtac gcgctctgct tcgtcctgtg ccaaacggac
     8761 gtcagtcgcg cccgctttca acaagatgta gttaacccgc tcgcagtctt cctgtacgtc
     8821 agactccacg ccgtccagtt cgcataacaa aatcgcttcg gcgtcgacgg gataaccggc
     8881 atgaataaaa tcttccgccg cgcggatcga caggttatcc atcatctcca gcccgccggg
     8941 gataatgcca ttggcgatga tgtcaccaac cgcaagtccg gctttttcta ccgagtcaaa
     9001 gctggctaac agaacccgcg ccacgggcgg cttcggcagc agttttaccg tcacttcggt
     9061 ggtcacgccg agcatacctt ccgatccggt gaacagcgcc agcaggtcaa aaccaggtga
     9121 atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc agcgtttgca cttcaatttt
     9181 cagcaggtta tgtacggtca gaccatattt caggcagtgg acgccaccgg cattttccgc
     9241 cacattgccg ccaattgaac aggcgatttg tgaggaagga tccggcgcgt aatagagatt
     9301 atgcggcgca acggcctggg agatcgccag gttacatacg cccggctgca cgcgcgcgcg
     9361 gcgaccaacg gggttaatgt cgaggctctc tttaaagcgc gccatcacca acaacacacc
     9421 tttttccagc ggcagcgcgc caccagaaag cccggtgcct gcaccacggg tcaccaccgg
     9481 tacacgcagg cgatggcaga cagccagaat cgctgtcacc tgttccattt gcttaggcag
     9541 aacaaccagt aatggacgcg tgcgatacgc gctcaacccg tcacactcgt aaggaatgat
     9601 ctcctcatcg gtatgcagga tctcaagtcc agggacatgc tcacgcagtg ccatcagtac
     9661 cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc tcttcgtaca agatgctcat
     9721 gagtaggctt cgctttgttg tgtggcagct gatttttgcg cgctgcttct gtgaacagtt
     9781 attaagcggg cttttcgttt tcgtctatct ctttagctac cggtcagacc attttttttc
     9841 cagctctgtg accttgtctt ggttaactta atgttaaatt gatgtaacat aatcacttgc
     9901 gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg tcctacctgt gcacgaggtc
     9961 cgggaatgaa agatgaacgt cgccctattt gcgaagtggt tgcagagagt atcgaacggt
    10021 taattatcga cggcgtactg aaggtcggtc agccgcttcc ctcggaacgt cgactgtgtg
    10081 aaaagctcgg cttctcacgc tccgcactgc gtgaagggct gaccgtgctg cgcgggcgcg
    10141 ggattattga aacggcgcag ggtcgcgatt ctcatgtcgc acggcttaat cgggtgcagg
    10201 acaccagccc gctgatccat ctgttcagta cgcagccgcg aacgctgtac gatctgctcg
    10261 acgttcgcgc attactggag ggcgaatcgg caaggctggc ggcaacgctg ggaacgcagg
    10321 ctgattttgt tgtgataacc cgctgttatg aaaaaatgct cgccgccagt gagaaccaca
    10381 aagagatttc gctgatcgaa catgcgcagt tggatcacgc tttccatctc gccatttgtc
    10441 aggcttctca caatcaggtg ctggtgttta cgctgcaatc attgaccgat ctgatgttta
    10501 attcagtgtt tgccagcgta aataatctct accatcgacc acagcaaaaa aagcagatcg
    10561 atcgccagca tgcgcggatc tacaacgcgg tgttgcagcg gctgccgcac gtcgcgcagc
    10621 gcgcagcgcg cagcgcgcga tcatgtacgg accgtgaaaa agaatctcca cgatatcgag
    10681 ctggaaggcc accatttgat tcgctcggcg gtgccgctgg agatgaacaa ggtgggaatg
    10741 tgagcagagt tttaccctat ccccaaggcg aggggaccgc ccctgcactt aacaggatct
    10801 ggtgttaaat ctgttcatct ataccctctc ccgaatgacc gcccctgcgc taaacaggat
    10861 ctggtattaa atttgctttt catacaatcg gtaacgcttg tacggctccg ccccaatgcg
    10921 ttccagcatg ttattcatgc ctgtattggt ttcgaggatc cattacatct ccagcgcatc
    10981 gatcttccgg cgggcaaacg gatcgcgtaa ggcttcaatc aataacagcg caatcaccgg
    11041 gccgatgcgg ctgaactgat actcgtcgcg cacgcccatc agcggtactc gcgcagttcg
    11101 cacaccgctg actttcaaac gccacagcaa ttttgcccag ccgaagggaa agagcgatcc
    11161 gttcagatcg gcaatcgcct cgttgatgtt aggcaagccg acaataaacg cgcagggtgc
    11221 agaatcaatc tcagcgatgt agatcatatc gtccggcacc agatatttaa gttggtcgcc
    11281 catggtcgcg aattcatgtt cggtaaacgg cacaaatccc cagttgtgct gccagccaga
    11341 gttgaaaatc tcacgcagga tctgcatctc ttcggcaaac cgctgacgat tgatgcagcg
    11401 aatggtcacc tttttgcgca cctgatccat ccgttttttt agcgccggag agaaagtgag
    11461 atcggttcgc tgcatccacc acgccagtaa atcaatgcct ttgtgataac ccagttgttc
    11521 aatatgcgcg gcataccacg gtttgccgtg tggcatcatc gcacagggtg gtgtgtcaaa
    11581 accttcaatc agtaatccgc tttcctgatt gatattcagg ctgaaaggcc cgcagatctt
    11641 acttgcacct tgtgacttca accacgcttc cgctgcgcca aacaacgcgg caaaatcctg
    11701 cggatcatca atggcgtcaa tcatgccgaa atgaccggta tcttcaccgt aacgctcacg
    11761 gtgcaaggta tctatttgcg cggtaatacg cccaactatc tgccccgctt tttttgccac
    11821 ccacgcctgc cagatgatat ggtccgtccc cggatttttc gcagacaaat gctcgttgcg
    11881 ttcaatgaat aaagggggta tccagtttgg atcgtcggga taaagtgatg acggaaaagc
    11941 gataaatgcc ttaaggtcat ttttattaat tactttttca attttaatca tcagcagatc
    12001 gcattccata tctctgtgga taacaaataa ttaatggtga aaaacaggtt gcaacagcaa
    12061 cgagcggcat cttaataatc aactcaatga atacaaacga cgaataaata gtattaatta
    12121 atgattatga taaggtcaga ggctacacag aaacgcctca atgccttttg tcgatttaag
    12181 gcgaccggac aaggcttctt cttaatatat tcataaatta aatatcattc ataataaaaa
    12241 taaaaaactc aaactcatta tttaaatcaa tgttcatttt cacaccgatg aaaatactat
    12301 ctcatttatc ttttatgcct atataatcca ccgcattatt gtcattttat ttttgatatc
    12361 caattttttc gtggtgtata tgtctaataa aatctttacg cattccctac ctatgcgcta
    12421 tgccgatttt ccaacgctgg ttgatgcttt ggactacgcc gctctgagta gagccggaat
    12481 gaatttttat gacagacgtt gccaacttga agatcaactg gaatatcaga cattaaaaac
    12541 gcgtgccgaa gctggtgcga agcggttgtt atcgctgaac ctgaaaaaag gcgaacgcgt
    12601 ggcgctgatt gccgaaacga gtagcgggtt tgtagaggct ttttttgcct gtcagtatgc
    12661 tggcttagtc gccttcccgt tggcgattcc aatgggcgtc ggtcagcggg attcctggag
    12721 cgccaaactc cagggtttac tggcaagttg ccagcccgca gccattatca ctggtgatga
    12781 gtagttgcca ctggtcaatg ccgcgacgca taacaacccc gaattacatg ttttaagcca
    12841 cgcttggttt aaggcattac cggaagccga tgttgcgctc cagcgtccag ttccgaacga
    12901 tatcgcctac ctccagtaca cctccggcag cacccgtttt ccccgtggcg tcattatcac
    12961 ccatcgcgaa gtgatggcta atctacgtgc tataagccac gatggcatta aattacgccc
    13021 tagcgaccgc tgcgtctcct ggcagccttt ctaccatgat atgggactgg tcggctttct
    13081 cctgcccccc gtcgccacgc aactttcagt agattatttg cgcactcagg attttgccat
    13141 gcgtcctttg caatggctta aattgatcag taaaaatcgc ggcaccgttt ccgttgcgcc
    13201 gccgtttggc tatgaattgt gccaacgccg cgtgaatgaa aaagatctcg ctgaactgga
    13261 tctttcctac tggcgcgtcg ctggtattgg tgctgagccg atctccgcag aacaactcca
    13321 tcaattcgct gaatgtttcc gtcaggttaa ctttgacaat aaaactttca tgccgtgcta
    13381 cggactggca gaaaatgcgc tggctgtcag cttctctgat gaagcctccg gggttgtggt
    13441 taacgaagtg gatcgcgaca tcctcgaata tcagggtaaa gccgtcgcgc cgggtgcaga
    13501 gacacgcgcc gtatcgactt tcgtcaactg cggcaaagcg ttgccggaac atggtattga
    13561 aatccgcaat gaagcaggta tgccggtcgc ggaacgtgtg gtaggccata tttgcatctc
    13621 cggtcccagt ctgatgagcg gttactttgg cgaccaggtt tcgcaagacg agattgccgc
    13681 gacgggctgg ttagacaccg gcgacctcgg ttatctgctg gacggttatc tgtatgtcac
    13741 cggacgcatt aaagatctga ttattattcg tggccgtaat atctggccgc aggatattga
    13801 atatatagcg gaacaggaac cggaaattca ttctggcgat gcgattgctt ttgttaccgc
    13861 ccaggaaaaa atcattttgc agatccagtg tcggatcagc gacgaagaac gtcgcgggca
    13921 gcttatccac gcgctggcgg cacggatcca aagcgaattt ggcgtgaccg cggctatcga
    13981 tctgttgccg ccccacagta ttccccgaac gtcctccggc aagcctgccc gtgcggaagc
    14041 gaaaaaacgt tatcagaagg cttatgctgc cagtcttaat gtgcaggaat ccctggcatg
    14101 aatcaaactg tcgcggtgac gggcgctacc gggtttatcg gtaaatatat tatcgataac
    14161 ctgctcgccc gcggctttca cgttcgcgca ttgacgcgta ctgcccgcgc tcacgtcaat
    14221 gataatctca cctgggtgcg cggttcgctg gaagatactc attcacttag cgagctggtt
    14281 gccggagcca gcgtagtggt ccattgcgcc gggcaagtgc gcgggcacaa agaagagatt
    14341 ttcacccgct gtaacgttga cggcagcctg cgcctgatgc aagcagcaaa agagagcggc
    14401 ttttgccaac gttttctgtt tatctcttcg ctggcggcgc gccatcccga gctctcctgg
    14461 tatgcaaaat ccaaatacgt cgccgagcaa cgactggcag caatggcgga cgaaattacg
    14521 ctgggcgttt ttcgcccgac agccgtgtat ggtcccggcg ataaagagtt aaaaccgctg
    14581 tttgactgga tgctacgcgg cctgctgcca cgacttggtg cgccagatac acaactctct
    14641 tttcttcacg tcaccgattt tgcgcaagca gtgggtcagt ggttaagcgc cgaaactgta
    14701 cagacgcaaa cctatgaatt atgcgatggc gtcgccggtg gctatgactg gcaacgcgta
    14761 cgacagcttg ccgccaaagc ccgttgtggt tccgtgcgaa tggttgacat tcctctgccg
    14821 gtactcacag gccttgcgga tatcagtacc gcgttgagtc gcctggcagg taaagaacct
    14881 atgctgaccc gctcgaaaat tcgtgaatta acccacgccg actggtcggc aagtaataac
    14941 cgtatttctg aagatatcaa ttggtttccc gggattagcc tggaacacgc attacgcaac
    15001 gggctatttt gattgtcttg ttttaaaggt gtgttaacgt ttctatggta aatcgtgaaa
    15061 tagtaatgga ttacatcctt tcctgtttac aggatttggt cgaaaacggt gtggaaatta
    15121 agcccgacag cgatctcgtc aacgatcttg gcctggagtc gatcaaagtg atggatctct
    15181 taatgatgct ggaagacaga ttcgatattt ccattcctat taatattctg ctgaatgtca
    15241 aaccccctgc gcaattaatg gaaactctac ttccatggct ggagaataaa taatggggct
    15301 atacgataaa tatgcgcgcc ttgctggcga gcgtctgcaa tttagtgaca acgggttaac
    15361 accgtttggc acctgtattg atgaagtgta ttccgccacc gaaggccgga tcggcaataa
    15421 aaaagtgatt ctggccggga ccaataatta tctcggttta acctttaatc atgatgccat
    15481 cagcgaaggt caggccgcgt tagcggccca aggcaccggc accaccggtt ctcgtatggc
    15541 aaacggcagt tatgcgccgc acctggcgtt agagaaagag attgccgagt ttttcaatcg
    15601 ccctaccgct atcgtttttt caaccggtta taccgctaat ttaggcgtca tcagcgcact
    15661 tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta tttatgacgc
    15721 ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta aagatctgga
    15781 acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg ttgagggcat
    15841 ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca aacgtcgtct
    15901 cggtggttat ttgattgtcg atgaagcgca ttcgtttgtc gtgttaggcg caacggggcg
    15961 cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg gtactttcag
    16021 taaaagtctg gcctcgattg gcggttttgc tgtcggttcg gaagcgatgg aagtgctgcg
    16081 ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca ttgcgaccgt
    16141 gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc tgatggataa
    16201 cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt cacatattag
    16261 cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct ggcgcgagct
    16321 tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg caggcatcac
    16381 gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg ccatcattca
    16441 ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc cacggcgggt
    16501 ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa acgctaccgg
    16561 taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat taaccatgat
    16621 ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac ggctacccat
    16681 cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca tcgccagggt
    16741 caggatgggt tgccccagtt tttcccacag agcaaggcgg tattcgctgc tgggttgccc
    16801 ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag tattccccgg
    16861 cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa gattatctaa
    16921 tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat gcaggaccca
    16981 cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa gcgagagatc
    17041 gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca gcgatttcac
    17101 tgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt catcgtcctc
    17161 ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg gcgacgccac
    17221 ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca gcgccaccag
    17281 tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac tgtttttcga
    17341 taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa gttcgaccag
    17401 tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat aaccgcccgg
    17461 gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca gcaatagccc
    17521 tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga aaacattcat
    17581 cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag gcccattagc
    17641 atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt agccaccagt
    17701 gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag tggcaataac
    17761 gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag cagcgccatc
    17821 aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc ggcaggaaag
    17881 gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg gctttgatcc
    17941 agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc acgcccctga
    18001 tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc gggtgatggg
    18061 tcaaccacat caaccgaacg ggcgcggaaa atacgggttc gattggcagt agaagtataa
    18121 atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag gatcattcgt
    18181 ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag ctccgactgc
    18241 gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata catcgacaga
    18301 agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta caagcgtccc
    18361 ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat ctgccctagc
    18421 gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc aatcagtact
    18481 ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc cgcttcggtc
    18541 agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac gataatcagc
    18601 accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc actaacgccc
    18661 tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat gacatgatct
    18721 acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc tttattcagc
    18781 accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg tccattgccg
    18841 tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt gccaacaaca
    18901 aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg ctgaaaacga
    18961 tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt tttcttcgcc
    19021 gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg tgtgctgttc
    19081 cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc ttcctggata
    19141 taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc cggggctaag
    19201 agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc tatagcggaa
    19261 aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc aacggtgcat
    19321 aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta tctcccatca
    19381 ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt tcaggatcaa
    19441 gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc acatcatgaa
    19501 attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc atttgctttt
    19561 gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat gaatagttca
    19621 ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa tacgacacaa
    19681 tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg gtcgttttgc
    19741 cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa cgcaggtgct
    19801 tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc tcatcgcctt
    19861 tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg agccagaatt
    19921 gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt gcaatagtcg
    19981 gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca aatagacgct
    20041 cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg ctgcgttata
    20101 aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga gcgccacgtc
    20161 cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca gatgacagaa
    20221 catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga gaaaagcgcg
    20281 ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg aaatcacaat
    20341 tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc caaacaccag
    20401 cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac cgattccgcc
    20461 cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg gatctaaacg
    20521 catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg cggcgtcgaa
    20581 gaatgtcatg gcgattttaa ataacccagc ggcagcgggt cctaacacga tccccaccaa
    20641 cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg ttgaccagac
    20701 aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt tgaaggcgtt
    20761 atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg tgccgccaac
    20821 cagattcgac acgtaccagg caataacaaa acccgcaaaa ccaaagtcaa aataccaggc
    20881 tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag caatcaaatc
    20941 aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg aaggaatgag
    21001 cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg aatgggaaag
    21061 gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga tatccagcga
    21121 gaaggaaaat gagacgatat tgcgaaattg ctgcggatta ttgttggtca aggctggcgt
    21181 tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga tcgacttggc
    21241 gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt taccggcaca
    21301 cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta accaggcgct
    21361 attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt taaaacccgc
    21421 caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt aagttaaact
    21481 tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt ccagcacttc
    21541 gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc caatcgtcga
    21601 catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc gggagatggc
    21661 agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg ccattttttg
    21721 gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg cacgttcgac
    21781 gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga agcgatcgct
    21841 gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg ccattcttcg
    21901 cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg ttttcatgct
    21961 ttgggttttc gaggatttgg ccgccagtcg ccgttccagc cagacgccaa ccaacggcaa
    22021 tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc gctcggtttg
    22081 ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac cggtgccatc
    22141 acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg ttgtactatt
    22201 aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat tttggtgatc
    22261 aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg gctggttgcc
    22321 gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta acggcgattt
    22381 tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt tgttcatcaa
    22441 tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac agcttcaact
    22501 cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc aattgcgggc
    22561 catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg agcagatagc
    22621 cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag gaaagcagat
    22681 agataaccag cgcagtaata ttgccaggcg gtgttgaggg cttttcgtgt acatgtgccg
    22741 ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt gaaatccatt
    22801 cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt cttgctgcca
    22861 gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat cctacaatgg
    22921 caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc acgaaaaacc
    22981 cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc gttcaacctt
    23041 attgtgacaa tggttaattg tgacattgac ctgaattacg ctttcataaa aacatattaa
    23101 ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca gtcaataaca
    23161 cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat
    23221 cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaggcc
    23281 ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt acataaaaca
    23341 attgaacgaa atcaggtgaa aacagcaaaa aaattgcccg gaccagttcc ggcgctggta
    23401 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
    23461 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
    23521 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
    23581 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
    23641 gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc gctggcgagg
    23701 aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
    23761 aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga ttttatgacc
    23821 gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
    23881 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaattgccgc
    23941 cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
    24001 gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg tggagacagg
    24061 catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg ccgccatgcc
    24121 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
    24181 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
    24241 agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt tctggtcttc
    24301 tttactgacg cctggcagtt tcgccagctt cgcggaggta tcagagatgc acagcataat
    24361 gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
    24421 cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc
    24481 tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg caatcaactt
    24541 ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg catcgcgggt
    24601 acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag gggcaatccc
    24661 caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcacgc cgtgcgaaaa
    24721 cgccacgcct gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt
    24781 gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc
    24841 actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc cgacgatagg
    24901 ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg catccatcac
    24961 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac caatcaaggt
    25021 gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga taatcgccgc
    25081 cagcagcatg gaaaagacca tcgccagacc gtgagttgac cccatattca gcaacaaatc
    25141 ggttggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
    25201 aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac gagtataaat
    25261 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
    25321 aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa caaataaatt
    25381 tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta
    25441 acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt
    25501 attagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact
    25561 ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat tgaatatttt
    25621 gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca acatgtatta
    25681 ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc
    25741 accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata cctgaatgta
    25801 tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa
    25861 tttaccgctg gttttgtcca gcacctcttc atgatggccg accaggtcga tattgctacc
    25921 acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat cattaacagt
    25981 gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat
    26041 cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt ccgggcgcag
    26101 caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc
    26161 aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt
    26221 ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc
    26281 cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc
    26341 cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg
    26401 acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt ccgccggatt
    26461 gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt
    26521 atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct catccatgca
    26581 gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg
    26641 tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt
    26701 gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta gctcctgctc
    26761 ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct gaccgttggc
    26821 ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt caaactggcc
    26881 tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt ccggctgcgg
    26941 taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa
    27001 agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc gcgtccactg
    27061 ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc
    27121 atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca
    27181 gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg
    27241 caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat
    27301 acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac
    27361 gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc
    27421 ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg
    27481 ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac
    27541 tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct
    27601 cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc
    27661 aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta
    27721 ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg
    27781 tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc tggtccgacg
    27841 catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct gggaacgccg
    27901 aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt tactggtgca
    27961 gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc
    28021 gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc
    28081 cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg
    28141 ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt acagggtgcc
    28201 gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt gaaaattgag
    28261 tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct ggataagcaa
    28321 ctggcgcagc ataagtttgt tgcgggcaat gagtacacca ttgcggatat ggcgatttgg
    28381 ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt tctggatgcg
    28441 ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc ggcagtgaaa
    28501 cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca tgagcgccat
    28561 gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg gttggtgttc
    28621 gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca
    28681 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca tcacgcgctg
    28741 gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg
    28801 atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag atcggcaggg
    28861 ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc
    28921 gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt
    28981 gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta gcgggtgttc
    29041 cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc tttttaggcg
    29101 ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc cggtatcgac
    29161 acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa tctccaccac
    29221 ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta aatcgcaccc
    29281 ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc tctcctcaac
    29341 gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat cgtgctgctc
    29401 cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat gcattttcgc
    29461 ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc ggattggtga
    29521 ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct tcttcaatcg
    29581 acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa acaccgtcca
    29641 gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt ttttcactca
    29701 ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc agcatccacg
    29761 gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa acatacaccg
    29821 gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg
    29881 ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca gtcatgaatc
    29941 gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac cagctcaagc
    30001 gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc cagcgattcc
    30061 gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg cagggccgac
    30121 aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa cgccggaact
    30181 tcttcatccc gcaggatcat catcgttccc ggcgtgttct ttttcgacac aatggcatcc
    30241 gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc cgccgtgccg
    30301 ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc ttcgacaatc
    30361 cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac taaaatacgc
    30421 attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg tgtaccgcac
    30481 aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt ttattcggat
    30541 cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca ttgaagttac
    30601 gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg ataccgtctt
    30661 taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca acacctttga
    30721 actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct ttgccgatat
    30781 tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag cagtgaacgg
    30841 tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc gtgttgccag
    30901 tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat tcgcgacctg
    30961 ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg ccgtagaaag
    31021 tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa gccggaatgg
    31081 tggtaccttc ccatggcgcc tgtggcgctt cgtctttata ccaggagtgc ttcgcgcttt
    31141 cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga tacgaggaca
    31201 gatccgcatt ctcaatgtag ccgcccggga acaggaagct gccgtttttg ctgtcggtcg
    31261 ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc cattccgggt
    31321 agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag tcgctcagtt
    31381 tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc aacccgtcga
    31441 ggttgattgg gttcgcgaca ccactcactg ccaggttctg aatgtgcggc gttttaccgc
    31501 ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg
    31561 ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc cagtagccat
    31621 tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc tgaactttag
    31681 tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt tcggaggctt
    31741 tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc gaaagctgat
    31801 agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt
    31861 gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg gaagacagcg
    31921 cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca tcgcgcggat
    31981 cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc catgctttcg
    32041 aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg
    32101 gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat gatcgttttg
    32161 ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc aaatctcaat
    32221 agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg cgtaaccgcc
    32281 gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac ctaacagtgc
    32341 gctcagtgct gacaggaaca gcatgcggga atcattacgc agcttcgcca cacgcagaac
    32401 gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt agaagtcacc
    32461 ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca gcccgatgaa
    32521 aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct tttcatccgg
    32581 accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa tcgagaagcc
    32641 catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct gccagagcgg
    32701 atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt gcatggtcgg
    32761 cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt gtagcgacac
    32821 cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca cgccgatgta
    32881 gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt gacccggaat
    32941 gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca agccacccag
    33001 tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt attgcccacg
    33061 gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc cggtgccaat
    33121 cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt cagagacaga
    33181 gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga ttaacggtcc
    33241 aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat catgactcat
    33301 gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc acggttaagc
    33361 ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga atattttcgg
    33421 aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca taaggcacac
    33481 ccgtcagtac cataacctga gtaccgccgc cctctttctc gccgtacaga tgcggataat
    33541 atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc ggatagtggt
    33601 attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg
    33661 taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga ccgcctttat
    33721 cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca ccaaacgggt
    33781 tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa cggcaaccgg
    33841 tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc agtgcagaga
    33901 ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc ttaatgtacg
    33961 cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac acctgaatga
    34021 tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt
    34081 tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag cctacgcaca
    34141 aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt tcagcagccg
    34201 catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct gctttaataa
    34261 aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct tttgctgacg
    34321 acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca caccgccgag
    34381 caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat ccggtttctg
    34441 tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat ggaagccgat
    34501 acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca caccgccaac
    34561 atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac agccgaggtg
    34621 gtacaggcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa aacgaccagc
    34681 atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg
    34741 acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc
    34801 gaggaagttg tacgggttcg gcgggcagcc cggaatattg ataacggttt tgcctggcag
    34861 aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa caccgcccca
    34921 cgcggagcag gaaccaatag cgataatggc tgctgcgcct tccgccgctt tgcggatgtg
    34981 atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta atgggatgga
    35041 accatccacc actaacacat actgcccttt gtacttctcg agagcgttat gtttgttctc
    35101 ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag agatagtctc
    35161 cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg tacaaccggt
    35221 gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa ccgattcggc
    35281 catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg cacaaagctt
    35341 catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc cagtcattta
    35401 tagttattcc gttgcgagga cctggcatat attttgcttc aatcgcaaaa tcaataatgc
    35461 gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat actgcggcgg
    35521 ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat aatacgtatg
    35581 tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga acaggaatta
    35641 ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg cgtcagaata
    35701 cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatccg gtgtgagtag
    35761 cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac cgcactgtcg
    35821 gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt catcgttcac
    35881 cccattcgtt atatatcgcc atatatagcg atattttagg caaagaacga cttttgcgca
    35941 cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct atcaggagag
    36001 atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag atttgtcata
    36061 acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat aagcctcata
    36121 aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt tgattcgggt
    36181 gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg
    36241 tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga cagccgccgc
    36301 gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg tcgcttgcgg
    36361 atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac
    36421 ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa cggaacttaa
    36481 tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca catcttcgat
    36541 atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt tgccgctcat
    36601 ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg aaggatacgt
    36661 gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca gcgcataatt
    36721 tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat acttcgccgc
    36781 aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt agtaatccaa
    36841 caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc tcaactggtg
    36901 tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact ctgtgagcca
    36961 gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga aattgaacgg
    37021 tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca gatgcagtac
    37081 cgctattgcg gaaaaagtgg tttacgcctg cccgcgttat cgctcggttt atggcacaat
    37141 ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc gtttgattta
    37201 ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag cgcagaagag
    37261 aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact gattatctct
    37321 accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc acgtaaatac
    37381 ctgctcgcca gcctcgacca aagcctaaag cgtatggggc ttgagtatgt cgatatcttt
    37441 tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc gctggctcat
    37501 gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc agagcggacg
    37561 caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca tcaaccttcg
    37621 tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct gcaaaataac
    37681 ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg aaaatatctc
    37741 aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaaattcg tggtctgacg
    37801 ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga aatggcacag
    37861 cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg
    37921 acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt gcaggcgctg
    37981 aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat cgccgatggc
    38041 gaactgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg gcgcgcgaac
    38101 cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc ccatcacaaa
    38161 tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt tatccgggac
    38221 atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc
    38281 tgctactttg tttttcagcg acgttgcgtc cattttcccc agccagttca gcttctcttt
    38341 gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca tcaccatcac
    38401 cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga tcaaatctga
    38461 ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga acttcagcgc
    38521 cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc
    38581 atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta aaacgggcaa
    38641 cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac gttaaacctt
    38701 tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc atcgagagcg
    38761 tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt taaaagaccc
    38821 gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc caggcatcca
    38881 ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta gcgctcgcct
    38941 gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg ctgccgccat
    39001 cgcttacgcg cgagaagggg ctgacgtggc gatcagttat cttcccgtgg aagaagaaga
    39061 cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc tgctgccagg
    39121 cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca aggcgttagg
    39181 cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg atattgcaga
    39241 cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc tgttctggct
    39301 aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt atcatcacca cttcgtcaat
    39361 ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg cggcgattct
    39421 gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg tgaatattgt
    39481 cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc aggataagat
    39541 cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg aactggcccc
    39601 tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag tgcacggcgt
    39661 gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca
    39721 ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca aacgcatgaa
    39781 gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc aggcattgag
    39841 cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac gaagattcaa
    39901 catgttgcaa tagattttgt aggcctgatg agcgtagcgt atcaggcaat tctactttac
    39961 ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc ctgatgcagc
    40021 gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg gaagaagatg
    40081 gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt ttcatcggtg
    40141 accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata aaccggtttt
    40201 tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac atctaccgtc
    40261 gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc gataaacggc
    40321 gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc
    40381 atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc agcggcgctg
    40441 gcttccagat ccaggtcacg gctttgcagc aacaatacct gcgctgcaac atcacccagc
    40501 atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac cgcaggaatc
    40561 gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg cgcaacgact
    40621 gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat gccccagacc
    40681 gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata gccgttaccg
    40741 cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct ggtacgttct
    40801 ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg cgcttcattg
    40861 agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt ggcctggttt
    40921 aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag
    40981 aactctacgc tcttactgaa gaagattgcc caggtgacta cggaggccaa aataagccca
    41041 atcatcacgc acttaacgac aatatcggcg tgctgataca taccccagac ggaaaggtcc
    41101 gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa aatctgcgca
    41161 taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg catgctgccg
    41221 tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc
    41281 gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc atgctagttt
    41341 agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc ttgatactca
    41401 actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata gcgtgattca
    41461 gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga cgcgcaatcg
    41521 cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt ccttacaaca
    41581 agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg gggcggcagc
    41641 ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga tgaccaacac
    41701 cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg gcgtaacgac
    41761 atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc caaacactaa
    41821 aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg ttccggcgat
    41881 tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca acacctgggc
    41941 agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc aggccgccac
    42001 caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca atgcccgttg
    42061 ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg ccgataccgc
    42121 ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag
    42181 cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgtgcgag ttaaccaccc
    42241 tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag gcagcagcgg
    42301 gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga actatctgga
    42361 taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt tgatcctggc
    42421 aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt ttagcgggac
    42481 cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg atctggacgc
    42541 cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga cattttattg
    42601 tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc aggagtacaa
    42661 taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt attcaagata
    42721 tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat attgttagcg
    42781 ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg cccgcctcat
    42841 ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa ggggttatgg
    42901 attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc tggctaagtt
    42961 atattcaggg gcgctggtta gggaatacca aaacggtgaa aggctggctg gcacagcttc
    43021 ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg ctggcgctgc
    43081 tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg ggaatttccg
    43141 gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg tgggtcagcg
    43201 tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc catgaagatc
    43261 aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc ttgttaggca
    43321 cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc ccgttcccgg
    43381 ctgctgtacc gggaacgtat ttaattcccc tgcattgccc gcattcttgc cgcatcttcc
    43441 cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc gctttcatag
    43501 ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat catccgcgcc
    43561 ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac agacttaaaa
    43621 ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc gacgctcagg
    43681 ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact gaagtgagtc
    43741 tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga
    43801 atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc catcacgtcg
    43861 agtaaccgct ccgccgcgaa taaaatctct tctgataacg tggcggagtt aatcccgctg
    43921 gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag ttcctgtaac
    43981 tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt ggcataggtt
    44041 tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc ataacgaaac
    44101 acgcg
//
LOCUS       DADLZM010000015        43871 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLZM010000015 DADLZM010000000
VERSION     DADLZM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.015"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..1227)
                     /locus_tag="JNY73_000851"
                     /pseudo
     CDS             complement(1..1227)
                     /locus_tag="JNY73_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            1621..2991
                     /locus_tag="JNY73_000852"
     CDS             1621..2991
                     /locus_tag="JNY73_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8046955.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAITAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3173..5327
                     /locus_tag="JNY73_000853"
                     /pseudo
     CDS             3173..5327
                     /locus_tag="JNY73_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5380..7110
                     /locus_tag="JNY73_000854"
     CDS             5380..7110
                     /locus_tag="JNY73_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046956.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQYVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(7170..7661)
                     /gene="yajQ"
                     /locus_tag="JNY73_000855"
     CDS             complement(7170..7661)
                     /gene="yajQ"
                     /locus_tag="JNY73_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY8046957.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7829..8740
                     /gene="panE"
                     /locus_tag="JNY73_000856"
     CDS             7829..8740
                     /gene="panE"
                     /locus_tag="JNY73_000856"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY8046958.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARTHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8703..9293
                     /gene="yajL"
                     /locus_tag="JNY73_000857"
     CDS             8703..9293
                     /gene="yajL"
                     /locus_tag="JNY73_000857"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY8046959.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDAQVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(9347..10795)
                     /gene="thiI"
                     /locus_tag="JNY73_000858"
     CDS             complement(9347..10795)
                     /gene="thiI"
                     /locus_tag="JNY73_000858"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY8046960.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            11001..11243
                     /gene="xseB"
                     /locus_tag="JNY73_000859"
     CDS             11001..11243
                     /gene="xseB"
                     /locus_tag="JNY73_000859"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY8046961.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            11243..12142
                     /gene="ispA"
                     /locus_tag="JNY73_000860"
     CDS             11243..12142
                     /gene="ispA"
                     /locus_tag="JNY73_000860"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY8046962.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            12167..14029
                     /gene="dxs"
                     /locus_tag="JNY73_000861"
     CDS             12167..14029
                     /gene="dxs"
                     /locus_tag="JNY73_000861"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY8046963.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTICVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            14084..15058
                     /gene="yajO"
                     /locus_tag="JNY73_000862"
     CDS             14084..15058
                     /gene="yajO"
                     /locus_tag="JNY73_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY8046964.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDDVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(15112..15630)
                     /gene="pgpA"
                     /locus_tag="JNY73_000863"
     CDS             complement(15112..15630)
                     /gene="pgpA"
                     /locus_tag="JNY73_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY8046965.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15608..16585)
                     /gene="thiL"
                     /locus_tag="JNY73_000864"
     CDS             complement(15608..16585)
                     /gene="thiL"
                     /locus_tag="JNY73_000864"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY8046966.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(16663..17082)
                     /gene="nusB"
                     /locus_tag="JNY73_000865"
     CDS             complement(16663..17082)
                     /gene="nusB"
                     /locus_tag="JNY73_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY8046967.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(17102..17572)
                     /gene="ribE"
                     /locus_tag="JNY73_000866"
     CDS             complement(17102..17572)
                     /gene="ribE"
                     /locus_tag="JNY73_000866"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY8046968.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17664..18764)
                     /gene="ribD"
                     /locus_tag="JNY73_000867"
                     /pseudo
     CDS             complement(17664..18764)
                     /gene="ribD"
                     /locus_tag="JNY73_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     gene            complement(18768..19217)
                     /gene="nrdR"
                     /locus_tag="JNY73_000868"
     CDS             complement(18768..19217)
                     /gene="nrdR"
                     /locus_tag="JNY73_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY8046969.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            19368..19907
                     /locus_tag="JNY73_000869"
     CDS             19368..19907
                     /locus_tag="JNY73_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY8046970.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            20206..21090
                     /locus_tag="JNY73_000870"
     CDS             20206..21090
                     /locus_tag="JNY73_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY8046971.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKIRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(21267..21614)
                     /locus_tag="JNY73_000871"
     CDS             complement(21267..21614)
                     /locus_tag="JNY73_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY8046972.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21743..22714)
                     /gene="secF"
                     /locus_tag="JNY73_000872"
     CDS             complement(21743..22714)
                     /gene="secF"
                     /locus_tag="JNY73_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY8046973.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22725..24572)
                     /gene="secD"
                     /locus_tag="JNY73_000873"
     CDS             complement(22725..24572)
                     /gene="secD"
                     /locus_tag="JNY73_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY8046974.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24600..24932)
                     /gene="yajC"
                     /locus_tag="JNY73_000874"
     CDS             complement(24600..24932)
                     /gene="yajC"
                     /locus_tag="JNY73_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY8046975.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24955..26082)
                     /gene="tgt"
                     /locus_tag="JNY73_000875"
     CDS             complement(24955..26082)
                     /gene="tgt"
                     /locus_tag="JNY73_000875"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY8046976.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(26137..27207)
                     /gene="queA"
                     /locus_tag="JNY73_000876"
     CDS             complement(26137..27207)
                     /gene="queA"
                     /locus_tag="JNY73_000876"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY8046977.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            27300..27873
                     /gene="acpH"
                     /locus_tag="JNY73_000877"
                     /pseudo
     CDS             27300..27873
                     /gene="acpH"
                     /locus_tag="JNY73_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(27878..29695)
                     /gene="malZ"
                     /locus_tag="JNY73_000878"
     CDS             complement(27878..29695)
                     /gene="malZ"
                     /locus_tag="JNY73_000878"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY8046978.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYLFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQSEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29851..31224)
                     /gene="proY"
                     /locus_tag="JNY73_000879"
     CDS             complement(29851..31224)
                     /gene="proY"
                     /locus_tag="JNY73_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY8046979.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKIAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(31300..32619)
                     /gene="brnQ"
                     /locus_tag="JNY73_000880"
     CDS             complement(31300..32619)
                     /gene="brnQ"
                     /locus_tag="JNY73_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY8046980.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(33026..34321)
                     /gene="phoR"
                     /locus_tag="JNY73_000881"
     CDS             complement(33026..34321)
                     /gene="phoR"
                     /locus_tag="JNY73_000881"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY8046981.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(34379..35068)
                     /gene="phoB"
                     /locus_tag="JNY73_000882"
     CDS             complement(34379..35068)
                     /gene="phoB"
                     /locus_tag="JNY73_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY8046982.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            35258..36460
                     /gene="sbcD"
                     /locus_tag="JNY73_000883"
     CDS             35258..36460
                     /gene="sbcD"
                     /locus_tag="JNY73_000883"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY8046983.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            36457..39600
                     /gene="sbcC"
                     /locus_tag="JNY73_000884"
     CDS             36457..39600
                     /gene="sbcC"
                     /locus_tag="JNY73_000884"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY8046984.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADMKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVELDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            39726..40907
                     /gene="araJ"
                     /locus_tag="JNY73_000885"
     CDS             39726..40907
                     /gene="araJ"
                     /locus_tag="JNY73_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY8046985.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(41050..41958)
                     /gene="mak"
                     /locus_tag="JNY73_000886"
     CDS             complement(41050..41958)
                     /gene="mak"
                     /locus_tag="JNY73_000886"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY8046986.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            42083..42994
                     /gene="rdgC"
                     /locus_tag="JNY73_000887"
     CDS             42083..42994
                     /gene="rdgC"
                     /locus_tag="JNY73_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY8046987.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(43072..43527)
                     /locus_tag="JNY73_000888"
     CDS             complement(43072..43527)
                     /locus_tag="JNY73_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8046988.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAAGRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(43524..>43871)
                     /locus_tag="JNY73_000889"
     CDS             complement(43524..>43871)
                     /locus_tag="JNY73_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY8046989.1"
                     /translation="LQVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPAS
                     LLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRK
                     IRSEARHRLEEKQ"
BASE COUNT        11426 a        11337 c        10671 g        10437 t
ORIGIN      
        1 tagttatact actaatttta tctgcattaa tttcaatact gacatcgcat attttggttt
       61 tttcatttgc gttaaagcca taggtcccgt aatgttttaa cgtgatgtaa ggtaaagtat
      121 caccctgtgt ttctcgggtt ttctgatctt gattacaacg ggttaaatat tcatccttat
      181 tgaatttcat tgaatatttc cccgcacctt tttcgtaata atcccatacc ttatcacaat
      241 caagcgtttc acctaatccg ttgtaataca tccaacttaa ccacaatgcg gcttttttgc
      301 cccttgctgt tcagccttgt caaaaagcat gctcgcttta gaataatcaa cctcaccggt
      361 caaaccagaa tagtatatat tccccagggc cactaatgct ttttcgtcat tcttatttgc
      421 agctaattcg taccattttt tggcttcgac agcatcaatc tttaaaccag cactgccatt
      481 ttcgtaggta tcgcccatgg tatactgcgc catcggataa cctttttctg ccgcagtacg
      541 aaaccattgc agtgctttct ttatggtgtc taaagaacgt ctatcatcag aaggtgaaaa
      601 atcatcgaga taaagatttc cgacaatata atccatttcg gggtcgcctt cctgcgccca
      661 tagagtcgcc agacgcagaa tttcaggttt actgaaacca attcgccccc aaaaaagcaa
      721 atcattctcg ggtgcctgac cgacataacg actgacaaat tcaattaaac tcgcccgttt
      781 cgttttgtct gtctcagccc cggatttcaa ttcataggcc tgattgatta ttttattcat
      841 caaatcaggc ggaatattaa gatcgtcagt tttcgactta ctggcatact tctgtgctcg
      901 tgtcacatct agggtgacgc ctttaccata ttgaaagaaa taacgtgcat tataacaacc
      961 gtattcgccc ccctggttac aggctttctc ataccactga atcgcctttg ggatgtcaac
     1021 agatactgtc actccgtgtt cataaataaa tcccagtagc actagcgcct gcgtgttatt
     1081 ggtatcagct aattttttca gttcttttaa cgccgtctga tctttattga caatacgttc
     1141 atacagttga tcaatatctg ttgaagcatg acaaaaagaa gccagagata aacccaatga
     1201 taataaaata actttaccag accacataca ttcctccttg atatattcaa ccaccaaaca
     1261 tccacctcat gaataaatac gctgccataa cggcccaggt aataagtaaa catttaagct
     1321 gaaacagcat aaccagtgcc tccaacgctt ctatttattg cgataaaata acaccatcct
     1381 ttgataaaaa gtcacacgcc catcaataaa tccaaaaata atgtcattag ttgcaggata
     1441 aaatcatttg agcttttcat ttgttatatt taacgcatga tgtattaatg gcaagaatat
     1501 gagagaaact catacctgac gtaaaaatca ctctcttatg taatatttac ttattaacac
     1561 tctgtttacc taacatgcgc tccgcactac actatgtggg caatttttta agaggtggta
     1621 atgaacgatt ataaaatgac gccaggtgag cggcgcgcga cctggggttt agggaccgta
     1681 ttttcattgc gcatgctggg catgtttatt gtactgccgg ttctgactac gtacggtatg
     1741 gcactgcaag gcgccagcga agcattaatc ggtattgcca ttggtattta tggtctgacc
     1801 caggccgttt ttcagattcc gtttggcctg ctttccgacc gcattggtcg aaaaccatta
     1861 attgtcggtg ggctggcagt gtttgccgcc ggtagcgtta tcgctgcgct ttctgactcc
     1921 atctggggaa ttattctggg ccgggcgcta caaggctccg gtgcgattac cgctgccgtt
     1981 atggcgctgc tttccgatct cacgcgcgaa caaaaccgta ccaaagcaat ggcgtttatc
     2041 ggcgtgagct ttggcattac ctttgccatt gcgatggtgc ttggcccgat catcactcac
     2101 aaacttgggc tgcacgcgct gttctggatg atcgctattc tggcaacgac cggcattgcg
     2161 ttgaccattt gggttgtgcc caacagtagc actcacgtac ttaatcgtga gtccggaatg
     2221 gtgaaaggca gtttcagtaa agtgctggcg gaaccgcggc tgctgaaact caactttggc
     2281 attatgtgtc tgcatatttt gctgatgtcg acgtttgttg ccctgcccgg acaactggct
     2341 gatgcggggt tcccggcggc tgaacactgg aaggtctatc tggcgacaat gctaatcgcc
     2401 tttggctcgg tcgtgccttt cattatctac gctgaagtta agcgcaaaat gaagcaagtc
     2461 tttgtcttct gcgtcgggtt gatcgtggtt gcggaaattg tgttgtggaa cgcgcaaacg
     2521 cagttctggc aactggtggt cggcgtgcag cttttctttg tggcgtttaa tttgatggaa
     2581 gccctgctgc cctcacttat cagtaaagag tcgccagcag gttacaaagg tacggcgatg
     2641 ggtgtttact ccaccagcca gtttcttggc gtggcgattg gcggttcgct gggcggctgg
     2701 attgacggca tgtttgacgg tcagcgggta tttctcgctg gcgcaatgct ggccgcagtg
     2761 tggctggcag tcgccagtac catgaaagaa ccgccgtatg tcagcagttt gcgcattgaa
     2821 atcccagaaa acgtaatggc agatgaaaaa ctggaaaaat gcttactgac aacgcctggt
     2881 gtcaaagatg tactgctggc gaagttagaa cattgcgtct atgtgaaagt agacagtaaa
     2941 atcaccaatc gttttgaaat agagcgagta atcaacgaag tatcttatta aataataagc
     3001 acggtgctat tcatgaaaaa taaaatagca ccgtacttcg taacaccata atcctttaaa
     3061 ccctccatta ttaatacttc gcttaattcc atataactgg acgattacat tagaagtaaa
     3121 ctatactttt tatgaaatat cagggataaa ttattcacat ggaaaataca tcatgtacaa
     3181 atcattattt actattatca ttctactgta tagcgccagt tcatatgcaa aaagcgaatc
     3241 ccctcccgca agcactataa ctttcattgc acaaatcgat catgttgagc taaataaaac
     3301 agcattatct catgatatga aactcgttct tgccgatcgt ttttatttca aaaccagtaa
     3361 tccttgcgaa acaaattcaa tttctgctcg accagaatca atggatatta ctgacaaagc
     3421 ctatgccaac aaattaaaaa aattagaaac tccttttctt gatgatcgct atttctttat
     3481 gaccatcaat cagtgtgacg gtaatggcac tcccatgctg acagacataa aagtatgtac
     3541 aacagaatta tgtggtgctg aatatacaaa gaaaatgtct actgtatggc tcgatgaaac
     3601 tttaaaacca actgctaaac gtctggcaat ttattttatt caatctcctt taccctatga
     3661 cgaggagaaa aaacaatgga aagtcaccgg atggtatatc tcatcaactg ataatcagtc
     3721 ttcaactaaa cagattgcat ttgaaggata tacagacaca gataacttct ccgattctaa
     3781 atttatttct tcatttaaat cctggtttga aagtggaaag ataaaaaaca ttgctaacta
     3841 taatccaaaa ggagaaagag atggaaaata cgaaagttat tacaatagca atggtaacgt
     3901 ctatgaatct ctaagttttt ctaatggtct tatcaacggt gagtttatta catattatga
     3961 aaatggggcc attgaaagta aacgtcattt taataacgac aaaattaaag atggtgaatg
     4021 ccctcatttt tatgaagatg ggaaaatcaa acagcaacat agctatctga atagtaaact
     4081 cgatggtcct gcatatgaat attatcctga tggcaaactc atgcaggaaa atttctatca
     4141 gcaaagtgtg ttgattggaa acgataccag ttactaccaa tccggaaaaa taagctctat
     4201 acacaataga aattcccgtg gtcagtatga tggagctaac gaacgttata gtgaagaggg
     4261 taagttacag tctaaatccg tctataaaga cggaaaacaa atttctgttc aaacctggta
     4321 tgaaaatggg cagaaagaag aggagaaaca ttttgatgag caaggccaat taaatagtct
     4381 ggttaagcaa tggtataaaa atggtaatct tgcaaaatca caaaactata aacatgacat
     4441 actcgatggt aacagtgaag agtggtacga aaatggcatt cctgaaagtt tgtaccctta
     4501 taagaatgga aaaaccgacg gcattgccaa atcatggaat aagtatggta aattaaccta
     4561 ttcaatcgaa tacaaaaatg gcgtagagaa tggggcatat cgtaactgga gcaaaaacac
     4621 aggtaaacta accaaagaaa ccctgtacgt caatggtatc agacagggag tggaaaaaga
     4681 gtttaacgat cgcacaggta aactattaac ctccacgcaa tatgttaata ataaaagaca
     4741 tggtacggag gaaacttatg atcaaaacgg aataaaatat attacatgct atcaaaatga
     4801 tcagaaatta tcgtctttag acaatccaac acaaataaaa gataatgcaa caacaggcga
     4861 ttccagtgct caatttgcat tagggaaata cgaattcatc tgtgcaaata tagatgaagg
     4921 gattaaatgg ttaaccaaat ctgcagagca aaaaaaacac tgatgcgata tactttttag
     4981 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     5041 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     5101 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     5161 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     5221 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     5281 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     5341 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     5401 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga ccccactcag
     5461 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     5521 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     5581 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     5641 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     5701 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     5761 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     5821 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     5881 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     5941 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     6001 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     6061 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     6121 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     6181 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     6241 caagactatc aaaacagcct ttcaaattac atagtattta tcgatgaaat ttttgccttt
     6301 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     6361 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     6421 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     6481 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     6541 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     6601 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     6661 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     6721 aatgatactg ccagcgcaaa ccgagtgttc catcagtatg tccctgcctt caatacagat
     6781 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     6841 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     6901 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     6961 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     7021 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     7081 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     7141 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     7201 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     7261 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     7321 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     7381 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     7441 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     7501 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     7561 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     7621 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     7681 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     7741 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     7801 ccggagtggt tgtgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     7861 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     7921 cgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     7981 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     8041 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     8101 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     8161 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     8221 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     8281 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     8341 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     8401 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     8461 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     8521 gatgcaggtg attgatgcca cagcggaaaa tatctcgtcg atgttgcagg atatccgcgc
     8581 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcacgca
     8641 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     8701 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     8761 ccacgatcga cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     8821 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     8881 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     8941 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     9001 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     9061 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     9121 aggacaagcg cgtcgtctgg gatgcacagg taaaattgct gaccagccag gggccgggta
     9181 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     9241 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     9301 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     9361 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     9421 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     9481 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     9541 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     9601 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     9661 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     9721 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     9781 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     9841 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     9901 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     9961 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
    10021 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
    10081 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
    10141 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
    10201 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
    10261 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
    10321 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
    10381 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
    10441 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
    10501 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
    10561 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
    10621 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
    10681 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
    10741 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
    10801 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
    10861 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
    10921 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
    10981 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
    11041 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
    11101 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
    11161 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
    11221 cttttacacc ggacaatgag taatggactt tccgcagcaa ttcgaagcct gcgttaagca
    11281 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
    11341 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
    11401 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
    11461 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
    11521 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
    11581 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
    11641 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
    11701 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
    11761 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
    11821 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
    11881 aagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
    11941 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
    12001 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcagtc
    12061 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
    12121 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
    12181 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
    12241 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
    12301 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
    12361 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
    12421 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
    12481 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
    12541 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
    12601 caccatctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
    12661 tcacgcgggc gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
    12721 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
    12781 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
    12841 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
    12901 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
    12961 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
    13021 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    13081 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    13141 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    13201 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    13261 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    13321 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    13381 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    13441 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    13501 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    13561 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    13621 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    13681 actggtgatc cttaactttg gtacgctgat gccagaagcg gccaaagtcg ccgaatcgct
    13741 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    13801 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    13861 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    13921 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    13981 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    14041 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    14101 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    14161 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    14221 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    14281 cgaagagatc gttggtcgcg cactgcggga tttcgcccgc cgtgaagacg tagtcgttgc
    14341 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    14401 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    14461 tcatcgcggg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    14521 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    14581 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    14641 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    14701 ggcggtgatt ccgtggagcc cgatggcgcg ggggcgactg acgcgtccat ggggagaaac
    14761 taccgcacga ctggtgtctg atgatgtggg gaaaaatctc tataaagaaa gcgatgaaaa
    14821 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    14881 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    14941 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccaga
    15001 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    15061 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    15121 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    15181 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    15241 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    15301 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    15361 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    15421 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    15481 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    15541 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    15601 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    15661 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    15721 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    15781 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    15841 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    15901 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    15961 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    16021 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    16081 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    16141 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    16201 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    16261 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    16321 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    16381 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca
    16441 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcagtcgt
    16501 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    16561 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    16621 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    16681 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    16741 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    16801 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    16861 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    16921 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    16981 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    17041 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    17101 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    17161 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    17221 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    17281 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    17341 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    17401 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    17461 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    17521 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    17581 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    17641 ttccctttcg agccgggaga gcatgccccc actaaatgca ggcaaacatc cgggcctaca
    17701 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    17761 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagttca
    17821 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    17881 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    17941 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    18001 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    18061 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    18121 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    18181 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    18241 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    18301 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    18361 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    18421 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    18481 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    18541 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    18601 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    18661 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    18721 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    18781 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    18841 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    18901 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    18961 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    19021 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    19081 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    19141 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    19201 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    19261 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    19321 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    19381 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    19441 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    19501 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    19561 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    19621 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    19681 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    19741 gaacaataga caactttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    19801 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    19861 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    19921 cgattttgtg cctcccgcaa catctttccc cgtcattttg ttactctgct tacatcactt
    19981 ggattgatag taaaagtttg caacaagggc gaaagtcagt acaatccccg cccgaatgtg
    20041 tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact gtgaaacgaa acatattttt
    20101 gtgagcaatg atttttataa taggctcctc tgtatacgaa atatttagaa acgcaatttg
    20161 cgcctttttc actcccgcaa gggattttca aacagtggca tacatatgaa aaaaacatta
    20221 ctggcagccg gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac
    20281 gacaaaccgc agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat
    20341 cacacccgtt tcggaccgca gatccgcaac gatacctacc ttgagtacga agcattcgct
    20401 aaaaaagact ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac
    20461 tccgatgcta aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccacgt
    20521 ttctccatcg acaagctgac caatactgac cttagcttcg gtccgttcaa agagtggtac
    20581 ttcgcgaaca actacattta cgacatgggt cgtaataaag atggtcgcca gagcacctgg
    20641 tacatgggtc tgggtaccga tatcgacact ggcctgccga tgagcctgtc catgaacgtc
    20701 tatgcgaaat accagtggca gaactatggc gcagcgaacg aaaacgagtg ggacggttac
    20761 cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt ggggcggtca gctgagctac
    20821 atcggcttca ccaacttcga ctggggttcc gatttagggg atgacagcgg taacgcaatc
    20881 aacggtatta agatccgtac taataactct atcgcttcca gccatattct ggctctgaac
    20941 tacgatcact ggcactactc tgtcgtagct cgttactggc acgacggtgg tcagtggaac
    21001 gacgatgcag aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt
    21061 ggttacctgg tagtaggtta caacttctga ttatgaaaat gccgggattt attcccggca
    21121 tttctgattg ttaacaacgt gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc
    21181 ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata acgtaggcct gataagcgta
    21241 gcgcatcagg caattttacg ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca
    21301 gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg catcttcccc tgcgataacg
    21361 gtcgtaccat actgatccgc ttcggtatat ttcgaatgct catgatcgtg gcaatagaga
    21421 cacaacaatt cccagttact gccatcttcc gggttattgg tatggtcgtg atcaatgtgg
    21481 tgaacggtaa gttcacgcag gttagaataa acaaactcgc gggaacagcg accgcagacc
    21541 cacggataga tttttaatgc tttttcgcga tagccgcttt ctaaccgtgc atagtttttt
    21601 gggatgatag ccatgaaaag ttacctggat gaaatgtagg gttacgcgca gtttcccaag
    21661 atgcggggat tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct gaccgggatt
    21721 ttcaacatca acgggaactt gattaaggca gaattgacgg ctgatccgcc ccttcttttt
    21781 ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc aacgcagatg
    21841 ctacgtagat ggaagatgca gtgccgatgg aaacaccgat aagcatggtc agcgagaagc
    21901 cttccagtac cggaccaccg aagagataca gcatcaggat aaccatcaag gtagtaccgg
    21961 atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg
    22021 taccgcgacg gatcttgcgg aagttttcac gaattcggtc cgataccacg atactgtcgt
    22081 taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca atctcgatat
    22141 ggaataacga caaaataccc agcgtaatga taacgtcgtg cgccagcgca ataaccaccc
    22201 ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac agcagcgctg
    22261 ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc cacgctcgga ccgacgaact
    22321 caatacgctt cactgctgca ttctgattgg tggattcgtt aatcaccttc agaacttggc
    22381 tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc atgatgtcgt
    22441 ggctgctacc aaagttttgc agcattggct cttcaaaacc ggctttttgc aatgcatcac
    22501 gcattacgtc aatttcagcc ggtttttcga gcgtaatttc gataaccgta ccaccggtga
    22561 aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcagcg attaacagca
    22621 gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact ttacggccgt
    22681 ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat tgacagcttc
    22741 ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac aatcgcggta
    22801 aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat tgccccggta
    22861 cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc gaagatagaa
    22921 ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac
    22981 tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc aagggttaag
    23041 acgatacccg cgatacctgg catactcagt gttgcgcctg gcaacagcga cataataccg
    23101 acgatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa cttcttatag
    23161 aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc cagcccctgc
    23221 tcaatgttct gcatacccag ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc
    23281 gcgatcaacg caccggcacg cagcagcagt gacagctgac gggcttcgtt cgggttgttg
    23341 atgccggtga tacggaagct gttacccaga cgagactgga tgttggcgat gttaatcacc
    23401 tcttcctgtt tcaccagaac cgcacgacca tttgcatctt tcttaccgct gtctttgtac
    23461 tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt agacataatg
    23521 ttgccaccag cgctatcgag cgagatgtta acctgcggct ggttgtattc gtcctggctg
    23581 gaagtggagt cggtgatatg gtcaccagtc agaattacgc gtttgtacag cacaaccggc
    23641 tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga tgccgcagcg
    23701 gcctgatcaa cattggtgtt taccagacgg aattccagcg ttgccgtcgc gcccagaatc
    23761 tctttcgcac gcgcagtgtc ctgaatacca ggcagttcaa caacgatacg gtcagcaccc
    23821 tgacgctgaa ccaccggctc cgccacgcca agttggttta cacggttacg caggatatta
    23881 atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct cattaccgca
    23941 cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt gctcagatac
    24001 gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc gtagttgttt
    24061 tctttacgaa cagtggtata cgggatgccc ttttcgcgca ggtcactgcg taggctatcg
    24121 atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat caggaagtga
    24181 acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc tgccagccag
    24241 cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat aacgcccatt
    24301 aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc
    24361 tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt ctggacctgg
    24421 atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac agccggatcc
    24481 tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac gatcagcatg
    24541 acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa aaattttaat
    24601 tacagcgcct tcatggtgcc tttcggcagg acggcagcta cgaagtcacg tttaataact
    24661 acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac tttggttacg
    24721 cgaccaacca ggccaccgtt cgtcagaact tcatcacctt tggcaatgga gtccatcagc
    24781 tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata gaaaatcaga
    24841 ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg tgcacccgtt
    24901 gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt aatattaatc
    24961 aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta cgaagctctc
    25021 taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt agtaacgaag
    25081 gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa
    25141 gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga gtgggccagt
    25201 atcgctctta tatttcgcat tgcggatttt caccacgcca tcggtcacga acaaatgacc
    25261 atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc gacgtacgcc
    25321 ttcaaccagg tcttccggtt taccaacgcc catcaggtaa cgcggtttgt ctgccggaat
    25381 ttgcggacac acatgctcca gaatacggtg catatctgct ttcggctcac ccacagccag
    25441 accgccgaca gcgtagccat caaagccgat atctaccaga cctttaacag atatatcacg
    25501 taaatcttcg taaatactgc cctgaattat accaaataac gcatttttgt taccaagact
    25561 gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatag agcgttttgc
    25621 gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga tatccgaacc
    25681 aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat
    25741 cgggttacgg aagtgaacgc cctgttcggt gattttacga atatcaccaa ggctgaagac
    25801 ctggaagccg ccggagtcgg taagaatcgg tcctttccac tgcataaaat cgtgcagatc
    25861 gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaaag tgttgccgag
    25921 gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta cggtgccgta
    25981 ggtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc
    26041 acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc cactacgtca
    26101 gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccgacgc gctcattaat
    26161 tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc
    26221 ttctaccgcc gctttatagg cattcatggt gtgttgataa ccggcaaagg ccgaaaccag
    26281 cataatcagc gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga ccactttgta
    26341 ctggaagccc ggatagataa agatttgggt atcgtcaaag aacggttcga taagatcgtt
    26401 ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa gtggtgccaa ccgcaatcac
    26461 ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg gtacttcagc
    26521 gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc
    26581 cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta atttttccag
    26641 caaaggctcg tcaaaatgca gaccagcggt cggggctgca accgcgcccg gtttttcgct
    26701 ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg
    26761 cagcggcata tggccgatgc tgttgaggat atccagtacc gagcgttcat cattaaattc
    26821 gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc
    26881 cagcaacagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg
    26941 tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg ctttacgccc
    27001 aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc cggggttgag
    27061 cttatcgagt aaatcggtga aagtaccgtg cgtcagcgca cccgtcggcc cgtccagcga
    27121 cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg attcgggcaa
    27181 ttcaaaggag aaatcggtaa cgcgcatgac actgactcag actaaaataa gagtcgggta
    27241 gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga atataatcga
    27301 tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt tctggcaatt
    27361 tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg
    27421 gcattcatat gcatcgacgt attgactgac aatctgccgg aagtccgcga agcacgggag
    27481 tggtttcgta gtgaaacgcg ccgcgttgcg cctattacgc tggatgtcat gtgggatcac
    27541 tttctttccc gccactggtc gcagctgtcg ccggactttc cgctacagga atttgtctgt
    27601 tatgcccgcg agcaagtgat gacgattttg ccggactcac cgccacgttt tatcaatctg
    27661 aacaattact tgtggtcaga gcagtggctg gtgcgctatc gcgatatgga tttcatccag
    27721 aacgtgttaa acggcatggc aagccgccgc ccacgtctgg atgccctgcg tgactcctgg
    27781 tacgatttag acgctcatta tgacgccctc gaaccccgct tctggcagtt ttatccgcgg
    27841 atgatggcgc aggcgtcaca caaggcgtta taaaacgtta gttcatccat accgtagccg
    27901 aaatggcagg caaagccaga atcccgtcag tcagttgccc atgcccttct ttgcattgcc
    27961 attgcacggc attgagaaac ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt
    28021 tgattgccac cagtacacgt tgctgattca gcacgcggac aaataccact acgttatctt
    28081 ccgcatacag cacctgacag ccgccacgac gcagcgcctg acttttctta cgcagcacaa
    28141 tcattcgccg gtacagcgcg aataacgccg tatcctgctt ttccacctgc caggggaacg
    28201 gtttacggca agacggatcg tttttaccat ccagtcccac ttcatcaccg taataaatgc
    28261 acggtacacc aggccaggtg aacagccaga ccaccgccag cggcaggcgc gcaatatccc
    28321 gaccgagcag cgttttaaat cgcgcagtat cgtggctatc gagctgatta aacatacgta
    28381 attgttgttg atgagaaagc cctgcgcggt aattatccat ccaggccata caggtttggg
    28441 cgtcaatttg ctgcggatca taagagatgt cggtattggc aagaaatccc cacaacggga
    28501 atgtgaagcc acgatagttc atggcagcat cttccacatc ggcctgtaac cattgccgtg
    28561 catcgccaaa atgttcgccg acaatatacg cttccgactg ggtttctttc gccgcttcgg
    28621 tgatcccggc aacgtgctgc atattattgc gcgccccacc cgcctccccc agcatatgca
    28681 ccacatccag ccgccagccg tccatattcc acggcgcttt cagccagtgg cggacaatac
    28741 tgtcttcccc gcgataaatt tcattcacca gactttccga ctgataatcc agcttcggca
    28801 agctggcata gccaagccag tcgagcgccg tgccatcatc actaaacaag taccagtcgc
    28861 gccagggcga ttcagagttg tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc
    28921 aggcatggga atcgccactg tggttaaaca cgccgtccag caccagccgc attcccagct
    28981 gctgcgtatt gtgtcgcaaa cgcagcaacg ccccatcacc gccaaactgc ggatcgacat
    29041 ggcgataatc ctcggtatcg tatttatgta cgctgggagc tttaaacacc ggattgagat
    29101 acagcgctgt cacgccaagc tttttcagat acggcagttt ttcgcttatc ccgtccagat
    29161 cgccgccata gaacgttgat ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca
    29221 agatgatctc ttgtccggct gcatgatggt aatagacatg atcctgttca gcttcacgag
    29281 gaagactacg cgcaaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt
    29341 gtgggccgat atccggtaca tcgacggcaa actgctccag tcgtgccggc ggcattcggc
    29401 tgaagccctg cggtgtaaac caacgctggc gatcgtgcca cagcagtttg aaactgtaac
    29461 gccgccgggg ttgtccgctg gagagatcaa tcgccgcacg ccatgcggtg acgcctggct
    29521 gcggctgact gcgctgctta tgcattggta ctgacatttc ttcgttatcg tgttctgtac
    29581 gcagcataat gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat
    29641 ctttgctttg tttaacaaat gggggcaccg gcaggtgcca tgcatttaac atcataattc
    29701 ccctgtgatg aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga
    29761 aggatatttg ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt
    29821 cctcctgaac cggcttacag aacgcaagta ttattggttt tcagccagct gacgatcgtg
    29881 gcggcgttta aacatccagc caatcaacag cacaacaatc cacgcgaaac cgacatacag
    29941 cgagatacgc gtatccgggt gataaccaat caacccgata ataaagagca ggaaaatcag
    30001 cccgccgatg gtcgttgcta ccccacccgg cactttaaat ttcagcgcct taacttcttc
    30061 tggcggcaaa cggcgacgga aggcgatttg cgacagcagg atcataatcc acacccatac
    30121 cgtggcgaag gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt
    30181 cagatacacc gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc
    30241 gcgacgcgac gttttgctga aaattttcgg tgcgctgccc tgctctgcca taccgtggag
    30301 catacggcct acgccaaata cgtcactgtt aattgccgac agcgaagcgg tcagcacaac
    30361 aaagttaaga atgctggcgg caaaggtaat gcccatatgc tggaacgtca gcacgaacgg
    30421 gctaccggca gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc
    30481 gacgtagaac accagaatac gcatcggcac ggagttaatc gcacgcggaa tcgatttctc
    30541 aggatctttc gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat
    30601 caccatttgc aacgacatta ccatgccaag ccagccgtta ctgaagaagc cgccgttgct
    30661 ccacaggtta tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc
    30721 gaaaccggcg acaatcatga tgatgatggt ggcgacttta aagaacgaga accagaattc
    30781 cagctcaccg aagaccttca cgctcatcag gtttacggcg caaatgatca gcaccacgct
    30841 cagtacccaa atccagtgcg gcaccgtcgg gaacaagaca cccatataga taccaaaagc
    30901 ggtcacatcg gcgatggcga caataaggat ttcaaagcag taggtccatc cggtaatgta
    30961 acctgccagc gggccaaggt tttcctgcgc ataacgcgag aaagagctgg cggccgggtt
    31021 atgtaccgac atttccccca gcgcacgcat aatgatatac gccgcgatac caccgataat
    31081 ataggccaac aacacgctcg gaccggctat tttgatggcg tctgccgaac cgtaaaacag
    31141 cccggtgcca attgctgaac ccagtgccat aaagcgtatg tggcgggtac ttagcccacg
    31201 ctttagctta ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg
    31261 ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac
    31321 ctgacgacct gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat
    31381 taaccacgcc agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc
    31441 gctgaatgca gatgctttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg
    31501 cggagcaatc acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag
    31561 tgcgatacac ggcggataaa tagcggtcag caccggtacg gagatctgga tcagctggct
    31621 taagccgagg ttagacacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata
    31681 agagagcggt acgtactggg caaagaattc tgcacaagca caggtcaggc caaccgccgt
    31741 gaccagacag gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt
    31801 gtgctgaacg taagcatgca gaatagcagc gccgtttgca gactgatcga ccagcgacgc
    31861 gctgtctgaa cccagacaga acagcgccag gtacagcaaa gtcagaccaa cacccgccat
    31921 caggccagcc cagacggtat aacgggtcag cagacgcgct tcggtaacgc cacgagaacg
    31981 cgccgcgtta acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata
    32041 gccgttaacg aagccgttag aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat
    32101 agaacccgcc ggccagacaa tcgcgacaac agacaggatg accagcgcga taattttcag
    32161 cggcgcaagg aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat
    32221 aacgatagcg aaatagacca ggctgtagat aaacagcggc agcgcggaat cacccgtcag
    32281 cggcgcaatc cccacttcaa aggaaacggt agctgtacgc ggcgtagcga aaagcggccc
    32341 caccgccagg taacaaaccg ttgccagcag tacgccagcg actttaccga ttggcgtgct
    32401 gaggctgtca acaccgccgc caacttttgc cagcgccact accgttaata ccggcaggcc
    32461 aacggcagta atgaggaagc cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc
    32521 gaccattgga gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc
    32581 cagagcgatg atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg
    32641 tggtgttgta acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa
    32701 acaattttag cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc
    32761 gcgttaatgc agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta
    32821 aagcaaggag ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata
    32881 aaacactatt tccgtgaaaa agacatggct aatcatgcga acaaaaaatc accaaccgtt
    32941 gtaaaatcta acgcccgtat tttgactgca aagaaaataa gccagcatcg gctggcttat
    33001 ggcaataaaa gatgacaaag gcggattaat cgctgttttt ggcaattaaa cgttccggga
    33061 taacaaaact gaaacgtgtt ccttttccta ctgtactctc aatattcagg cgactttcgt
    33121 gatgattcac cgcatgtttc acgatcgcta accctaatcc gctaccgccg gtttgccggg
    33181 aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa
    33241 tgcccggtcc gttatcttca acgctaaatt cggcaccgtg cggcactcgc tgccagcgta
    33301 cggtgatatg cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg
    33361 cactgcgtag ctgatcttcg ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa
    33421 atgtctgttt tttctgactc agagtctgag cctcgcgctc aacaacgcgc agcatcatcg
    33481 gcacatcaac cttttcattg agcaattgcg tcggcgaggc ttcaattttc gacagcgtta
    33541 gcaactgctt caccagccct tccatccgct gggtctgctc gcgcatggtg tgcaacgctt
    33601 tttcgcgtac cgcgccttcc agcggctgct catccatcat ctccaggtaa ccctgtaaca
    33661 cggtcaatgg cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt
    33721 ccagttgatg catttgcgtg acatcacgcg ccaccatcaa caactgtttg tgggtataag
    33781 gcatgacgcg aatttccaga tgccgcccgg tgttgagcac cagattgagc gggcgagaaa
    33841 aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg cagtaggtta aggatgttct
    33901 gcccgttatc ttccggccag cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga
    33961 aaataccgcc ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac
    34021 gaaagcgttt aatcagattg cccagttcac ggcggcgttt tttatttcgc agctgcatct
    34081 ggtgtaagcc gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactgc
    34141 gatccaccca cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc
    34201 ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag
    34261 ccgggaggca gcaaagtagc agctccagca ccagcctttt ccacgacagc cgttccagca
    34321 cgtaagatac tccagttaag aaatcataag ccctgctctg cgtccgatga gcaaggcgtt
    34381 aaaagcgggt tgaaaaacga tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc
    34441 cgccgggctc cagtgcttta cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca
    34501 catacacgtt agttccccag acgtggttta acagctgctc gcggctgtac acgcgctcag
    34561 gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc agcggctctt
    34621 cgcccgccat cactcggtga gatgtcgggt cgagacttaa tccctgcatc tcaatcacct
    34681 cttccaccgc cattggcgaa atacggcgca ttaccgcttt gattcgcgcc accagctcct
    34741 tcggcgaaaa tggcttggtg atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat
    34801 cttcttcttc ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc
    34861 gcttgaggtg tttgatgaac tggataccgg agccgccaag taacatccag tcaaggagaa
    34921 ttaaatccgg ccaaggttca ttcagttgat tcacagcact gtcataatct tccgcttcga
    34981 ccggctgaaa gccattttgt tcgagcacga agcagaccat ttcgcgaatt ggagcttcat
    35041 cttctacgac cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat
    35101 cattaatgcg acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac
    35161 atatcttcag gttattgatt tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg
    35221 tataatcgcg accatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga
    35281 ctggcatctc ggccagaact tctacagtaa aagccgcgaa gctgaacatc aggcttttct
    35341 tgactggctg ctggagacag cacaaaccca tcaggtggat gcgattattg ttgctggtga
    35401 tgttttcgat accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt
    35461 caatttacag caaactggct gccatctggt ggtactggca ggaaaccatg actcggtcgc
    35521 cacgctgaat gaatcgcgcg atatcatggc gttcctcaat acaaccgtgg tcgccagcgc
    35581 cggacatgcg ccgcaaatct tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc
    35641 cattccgttt ttacgcccgc gtgacattat ttccagccag gcggggctta acggtattga
    35701 aaaacagcag catttactgg cagcgattac cgattattac caacaacact atgccgatgc
    35761 ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc acgggacatt taacgaccgt
    35821 gggtgccagt aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc
    35881 ggcacaaaac tttccaccag ccgactacat cgcgctcggg catattcacc gcgcacagat
    35941 tattggcggc atggaacatg ttcgctattg cggttccccc attccactga gttttgatga
    36001 atgcggtaag agtaaatatg tccatctggt gacattttca aacggcaaat tagagagcgt
    36061 ggaaaacctg aacgtaccgg taactcaacc catggcggtg ctgaaaggcg atctggcgtc
    36121 gattaccgca cagctggaac agtggcgcga tgtatcgcag gagccacctg tctggctgga
    36181 tatcgaaatc actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac
    36241 cgaatcattg cctgtcgaag tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt
    36301 gttagccagc caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg
    36361 ccgtctggca ctggaagaac tggatgaatc gcagcagcaa cgtctgcagc atcttttcac
    36421 cacgacgttg cataccctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg
    36481 aaaaacctga actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc
    36541 agcaacgggc tgtttgccat taccggccca accggtgcgg ggaaaaccac cctgctggac
    36601 gccatttgtc tggcgctgta tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa
    36661 aatgatctca tgacccgcga taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa
    36721 ggtgaagcgt atcgcgcgtt ctggagccag aatcgggcgc gtaaccagcc ggacggtaat
    36781 ttgcaggtgc ctcgcgtaga gctggcgcgc tgcgccgacg gcaaaatcct cgccgacaaa
    36841 gtgaaagata agctggaact gacagcgacg ttaaccgggc tggattacgg gcgcttcacc
    36901 cgttcgatgc tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa
    36961 cgcgcggaat tgctcgagga gttaaccggc actgaaatct acgggcaaat ctcggcgatg
    37021 gtttttgagc agcacaaatc ggcccgcact gagctggaga agctgcaagc gcaggccagc
    37081 ggcgtcacgt tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt
    37141 actgacgaag aaaaacagtt aattaccgcg cagcagcaag aacaacaatc gctaaactgg
    37201 ttaacgcgtc aggacgaatt gcagcaagaa gccagccgcc gtcagcaggc cttgcaacag
    37261 gcgttagccg aagaagaaaa agcgcaacct caactggcgg cgcttagtct ggcacaaccg
    37321 gcacgaaatc ttcgtccaca ctgggaacgc atcgcagaac acagcgcggc gctggcgcat
    37381 attcgccagc agattgaaga agtaaatact cgcttacaga gcacaatggc gcttcgcgcg
    37441 agcattcgcc accacgcggc gaagcagtca gcagaattac agcagcagca acaaagcctg
    37501 aatacctggt tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg
    37561 cgtgcgcagt tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag
    37621 ttaacccatg ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac gttaaccgcc
    37681 gatgaagttg ctaccgccct ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg
    37741 gtcgcgctgc atgggcagat tgttccccaa caaaaacgtc tggcgcagtt aatggtcact
    37801 atccagaatg tcactctgga acagacgcaa cgtaatgtcg cacttaacga aatgcgccag
    37861 cgttataaag aaaagacgca gcaacttgcc gatatgaaaa ccatttgcga gcaggaagcg
    37921 cgcatcaaaa cgctggaagc ccagcgtgca cagttacagg cgggtcagcc ttgtccactt
    37981 tgtggttcca ccagccaccc ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat
    38041 cagtctcgat tactggcgct ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg
    38101 ctgcgcgggc aactggatgc attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa
    38161 agcctccgac aagatgagca agcacttact caacaatggc aagccgtcac ggccagcctc
    38221 aatatcacct tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac
    38281 gaacgccagc tgcggttact cagccaacga catgaattac aagggcagat tgccgcgcat
    38341 aatcagcaaa ttatccagta tcaacagcaa attgaacaac gtcagcagca gctcttaacg
    38401 gcattggcgg gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg
    38461 acacgtcagc aagaagcgca gagctggcag caacgccaga acgaattaac cgcgctgcaa
    38521 aaccgtattc agcagctgac gccgattctg gaaacgttgc cgcaaagtga tgatctcccg
    38581 cacagcgaag aaactgtgga gctggataac tggcggcagg ttcatgaaca atgtctcgca
    38641 ttacacagcc agcagcagac gttacagcaa caggatgttc tggcggcgca aagtctgcaa
    38701 aaagcccaag cgcagtttga caccacgcta caggccagcg tctttgacga tcagcaggcg
    38761 ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc tggaacagct caagcagaat
    38821 ctggaaaacc agcgccgtca ggcgcaaacg ctggtcactc agacagcaga aacgctggca
    38881 cagcatcaac aacaccgacc tgacgggttg gctctcactg tgacggtgga gcagattcag
    38941 caagagttag cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt
    39001 cgccagcagc tgaagcagga tgcagataac cgtcagcaac aacaaacctt aatgcagcaa
    39061 attgctcaaa tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc
    39121 aaagagggcg ataaattccg caagtttgcc caggggctga cgctggataa tttagtccat
    39181 ctcgctaatc agcaacttac ccggctgcac gggcgctatc tgttacagcg caaagccagc
    39241 gaggcgctgg aagtcgaggt tgttgatacc tggcaggcag atgcggtacg cgatacccgt
    39301 accctttccg gcggcgaaag tttcctcgtt agtctggcgc tggcgctggc gctttcggat
    39361 ctggtcagcc ataaaacacg tattgactcg ctgttccttg atgaaggttt tggcacgctg
    39421 gatagcgaaa cgctggatac cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa
    39481 accatcggtg tgattagcca cgtagaagcg atgaaagagc gtattccggt gcagatcaaa
    39541 gtgaaaaaga tcaacggcct gggctacagc aaactggaaa gtacgtttgc agtgaaataa
    39601 ctattcagca ggataatgaa tacagagggg cgaattatct cttggccttg ctggtcgtta
    39661 tcctgcaagc tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg
    39721 gtggtatgaa aaaagtcatt ttatctttgg ctctgggcac gtttggtttg gggatagccg
    39781 aatttggcat tatgagcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg
    39841 ctgccgggca tatgatctcg tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg
    39901 cactcttttc cagccgctac tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg
    39961 tcattggcaa cgccatgttc acgctctctt cgtcttacct gatgctcgcc attggtcggc
    40021 tggtatccgg ctttccgcat ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa
    40081 ttatcaaacc cggaaaagtc accgccgccg tggcggggat ggtttccggg atgacagtcg
    40141 ccaatttgct gggcattccg ctgggaacgt atttaagtca ggaatgctgg cgttacacct
    40201 ttttattgat cgctgttttt aatattgcgg tgatggcatc ggtctatttt tgggtgccag
    40261 atattcgcga cgaggcgaaa ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg
    40321 ccccgtggtt aattttcgcc gccacgatgt ttggcaacgc aggtgtgttt gcctggttca
    40381 gctacgtaaa gccatacatg atgtttattt ccggtttttc ggaaacggcg atgaccttta
    40441 ttatgatgtt agttgggcta gggatggtgc tgggaaatat gctaagtggc aggatttcag
    40501 gacgttattc accactgcgc attgcagcag tgactgactt tatcattgta ctggcactgc
    40561 tgatgctctt tttctgcggc ggcatgaaaa caacgtcgct tatttttgct tttatttgtt
    40621 gcgcgggatt atttgccctt tcagcacctc tgcaaatatt gttactgcaa aatgccaaag
    40681 gcggagagtt attaggtgcc gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg
    40741 gcgcatattg cggaggtatg atgctgacgc tggggctggc atataattac gtggcgctgc
    40801 ctgccgccct gttttcgttt gctgcgatgt catcgttgct gctgtatggt cgctataagc
    40861 gccagcaagc ggcggatagt ccggtgctgg cgaaaccact ggggtagatt actgtcacgg
    40921 tgcgcgttgc ttattgccgg atgcggcgtg aacgccttat ccgccctacg cggttctggc
    40981 acattttgta ggcctgataa gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc
    41041 ctacgttttt tactcttgtg gccataacca cgcagcgccg cgtacgccgc tggaatcacc
    41101 gtgcttcgcc ttactcaccg gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa
    41161 ctgcccaacc gtttgatata aacggtctac attgctcatc ccgcccccca ggacaatcac
    41221 atccggatcg agaatattca cgacatgtgc cagcgatttt gccagccgca gctcgtagcg
    41281 acgcaatgcc agttccgcta ccggatcgct ttcttcaacc aggcggataa tttcactgcc
    41341 tttcagcgca tgtccgctca aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa
    41401 ggtttcaata caaccttgtt taccgcaata acaagggact tcctcgcgat aacgcagttc
    41461 gtcttcgtcc atccacggta gcggattgtg tccccactca cctgccgtgc cattgccgcc
    41521 gatatgcgtc cgcccattga atgccacgcc cgcgccgcat cccgtgccga taatcacggc
    41581 aaataccgtc tgcgctcccg ctgccgcgcc atctactgct tctgaaaccg ccagacagtt
    41641 ggcatcattt gccagccgca cttcccgctg caacctcgcg cttaagtctt tatcgaatgg
    41701 ctgaccgttg agccaggttg aattggcatt cttcaccaca ccggtgtagg gcgaaatcga
    41761 acctggaatg cccataccta ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac
    41821 caacgtggcg atcgtttcaa tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg
    41881 gcggtacaac tgctcccctg catcgcccag tgcaatcact tcagttttgg tgccgcctaa
    41941 atcgatacct atacgcactg tactctcctt atttttttca atatcaatag cgtagagacg
    42001 gacaaccgga ttggcaatgc aaggccgcct acaattcgtt atcatgcccg ctaaatttaa
    42061 cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat ttaatggttt accgtcttag
    42121 ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag ctagcctcga tggcatttac
    42181 cccatgcggc agccaggaca tggcgaagat gggctgggtt cctccgatgg gatcgcacag
    42241 cgatgcgtta acgcacgttg ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa
    42301 aatcctcccg tctccggtga ttaaacaggc gctggaagcg aaaatcgcca aactggaagc
    42361 ggaacaggcg cgtaagctga agaaaaccga aaaagattcg ctgaaagacg aagtgctgca
    42421 ctctctgctg ccgcgtgctt tcagccgttt tagccagaca atgatgtggc tcgacacggt
    42481 taacggtttg attatggtgg actgcgccag tgccaaaaaa gcggaagata cgctggcatt
    42541 actgcgtaaa agcctggggt cgttaccggt tgtaccattg agcatggaaa acccgattga
    42601 actgacgctg accgaatggg ttcgctccgg tagtgcggca cagggcttcc agctgcttga
    42661 tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg atccgcgcga agaaacaaga
    42721 tctgaccagc gaagagatca ccaatcacat tgaagccgga aaagtggtga ctaaactggc
    42781 gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct
    42841 taagttctgc gacgagctgc acgatcaaaa cgaagatatc gaccgtgaag atttcgccca
    42901 gcgttttgat gccgatttca tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct
    42961 gattgaagga ttaggtggcg aagcacaacg ttaattgctg attttccttt aatgccggat
    43021 gcgacgcctg ccgcgtctta tccggcgtac gaagccacac caggcatata attattcgct
    43081 acggcgagca ataattttta gcgcagcaat attatgcgtt ttacgccgta actgatctaa
    43141 tcgcctcatc cctattcgcc tgccagcagc acgcagaata ttcactaacc cccatttatc
    43201 gctggtaaat tgttttgtta acagctccgc ttttggggta tcaatatatt cacgcaataa
    43261 atcagaaatt gaacaaatat ccgtcaggta aggataccag caagtttcac cgtgaaaatt
    43321 acgcactcca gcgttacctg caataaagtc tttcgggtaa tcccagatca cgtttttatc
    43381 caacgtgatc caataacgcg gtaaatcggt acttccacca ttttggctgc gcatgggata
    43441 acgggtacag tgaatttgca ggttgatcgt tggcgtcagt aagtcataga tctcccgctg
    43501 taacttgctc catggacgtt gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg
    43561 cgaattttcc gcgttgcccc ggttttcact aaatcacgta actgcgaaag atcgggttgt
    43621 tcgacaaata agagtaatga tggatcctct tttaaccacg ccgttttcac atcggcagcc
    43681 agctgtggat tattaatagc cagcgaccga tcctgaggct cagtcaatgt cgttaacaac
    43741 gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc
    43801 agcaatgcac gccaccattc atcatctgat ttatctttta tcgtagccag ttccgcaaaa
    43861 aatacctgga g
//
LOCUS       DADLZM010000016        43461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLZM010000016 DADLZM010000000
VERSION     DADLZM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.016"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..413
                     /locus_tag="JNY73_000890"
     CDS             <1..413
                     /locus_tag="JNY73_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8046990.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="JNY73_000891"
                     /pseudo
     CDS             438..3037
                     /locus_tag="JNY73_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="JNY73_000892"
                     /pseudo
     CDS             3028..4098
                     /locus_tag="JNY73_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4110..4652
                     /locus_tag="JNY73_000893"
     CDS             4110..4652
                     /locus_tag="JNY73_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8046991.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4660..5175
                     /locus_tag="JNY73_000894"
     CDS             4660..5175
                     /locus_tag="JNY73_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8046992.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="JNY73_000895"
     CDS             5141..5878
                     /locus_tag="JNY73_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY8046993.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="JNY73_000896"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="JNY73_000896"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY8046994.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="JNY73_000897"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="JNY73_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY8046995.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNY73_000898"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNY73_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY8046996.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNY73_000899"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNY73_000899"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY8046997.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPSATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="JNY73_000900"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="JNY73_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="JNY73_000901"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="JNY73_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY8046998.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQLLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="JNY73_000902"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="JNY73_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY8046999.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="JNY73_000903"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="JNY73_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY8047000.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="JNY73_000904"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="JNY73_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY8047001.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNY73_000905"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNY73_000905"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY8047002.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="JNY73_000906"
     CDS             complement(17760..19520)
                     /locus_tag="JNY73_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY8047003.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="JNY73_000907"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="JNY73_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY8047004.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNY73_000908"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNY73_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY8047005.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNY73_000909"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNY73_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY8047006.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="JNY73_000910"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="JNY73_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY8047007.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNY73_000911"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNY73_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY8047008.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="JNY73_000912"
     CDS             complement(25127..25573)
                     /locus_tag="JNY73_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY8047009.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="JNY73_000913"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="JNY73_000913"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY8047010.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNY73_000914"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNY73_000914"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY8047011.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="JNY73_000915"
     CDS             complement(28336..28998)
                     /locus_tag="JNY73_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY8047012.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="JNY73_000916"
     CDS             29171..29584
                     /locus_tag="JNY73_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY8047013.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNY73_000917"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNY73_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY8047014.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNY73_000918"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNY73_000918"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY8047015.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="JNY73_000919"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="JNY73_000919"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY8047016.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNY73_000920"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNY73_000920"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY8047017.1"
                     /translation="MLIFEGKEIEMDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNY73_000921"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNY73_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY8047018.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="JNY73_000922"
     tRNA            complement(32850..32937)
                     /locus_tag="JNY73_000922"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="JNY73_000923"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="JNY73_000923"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY8047019.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36253
                     /gene="hyaB"
                     /locus_tag="JNY73_000924"
                     /pseudo
     CDS             34478..36253
                     /gene="hyaB"
                     /locus_tag="JNY73_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36272..36979
                     /gene="hyaC"
                     /locus_tag="JNY73_000925"
     CDS             36272..36979
                     /gene="hyaC"
                     /locus_tag="JNY73_000925"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY8047020.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36976..37563
                     /locus_tag="JNY73_000926"
     CDS             36976..37563
                     /locus_tag="JNY73_000926"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY8047021.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37560..37958
                     /gene="hyaE"
                     /locus_tag="JNY73_000927"
     CDS             37560..37958
                     /gene="hyaE"
                     /locus_tag="JNY73_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY8047022.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37955..38812
                     /locus_tag="JNY73_000928"
     CDS             37955..38812
                     /locus_tag="JNY73_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY8047023.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38946..40490
                     /gene="appC"
                     /locus_tag="JNY73_000929"
     CDS             38946..40490
                     /gene="appC"
                     /locus_tag="JNY73_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY8047024.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40502..41638
                     /gene="appB"
                     /locus_tag="JNY73_000930"
     CDS             40502..41638
                     /gene="appB"
                     /locus_tag="JNY73_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY8047025.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41650..41742
                     /gene="cbdX"
                     /locus_tag="JNY73_000931"
     CDS             41650..41742
                     /gene="cbdX"
                     /locus_tag="JNY73_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY8047026.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41822..43120
                     /locus_tag="JNY73_000932"
     CDS             41822..43120
                     /locus_tag="JNY73_000932"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY8047027.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43235..>43461)
                     /locus_tag="JNY73_000933"
     CDS             complement(43235..>43461)
                     /locus_tag="JNY73_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047028.1"
                     /translation="AVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIK
                     GAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10809 a        10587 c        11566 g        10499 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttgctg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacga ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gctagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggcgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcggacg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccggact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctagcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagctgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt gaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccaccga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcaggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggatgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagta gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 catttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgcaaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacatat
    34981 ttcttcgacg tacaaaatcg cctgaagaaa ttcgttgaag gcgggcagtt ggggatcttc
    35041 cgcaatggct actgggggca cccgcagtac aaattgccgc cagaagccaa cctgatgggc
    35101 tttgcccact atctcgaagc tctcgatttc caacgtgaaa ttgtcaaaat tcacgcggtc
    35161 tttggcggta aaaacccgca tccaaactgg attgtcggcg ggatgccttg cgccattaac
    35221 attgacgaaa gcggcgcggt cggggcggtc aatatggaac gcctgaacct ggtgcagtcg
    35281 attatcaccc gcacggcaga cttcattaac aacgtgatga tccccgacgc cttagccatc
    35341 ggtcagttca acaaaccgtg gagcgaaatc ggcacgggtc tttctgataa atgtgtcctc
    35401 agctacggcg cattcccgga tattgccaac gactttggtg agaaaagtct gttgatgcct
    35461 ggcggcgcgg tgattaacgg cgacttcaac aatgtgctgc cagtggattt ggttgatccg
    35521 cagcaggtgc aggagtttgt cgatcacgcc tggtatcgtt atcccaacga tcaggtcggg
    35581 cgtcatccat tcgatggcat taccgacccg tggtacaacc ccggcgatgt caaaggcagc
    35641 gataccaaca ttcagcagct gaatgaacag gaacgctact cgtggatcaa agcgccacgc
    35701 tggcgcggta acgcgatgga agtggggccg ctggcacgca cgttaatcgc ttatcacaaa
    35761 ggcgatgctg cgaccgttga gtcggtcgat cgcatgatgt cggcgttgaa cctgccgctt
    35821 tccggtatcc agtcaacgtt gggccgcagg taagttgcag tatttcttcg acaagctgat
    35881 gaccaacctg aaaaatggca atctcgccac tgcttccact gaaaaatggg aacctgcaac
    35941 ctggccgaca gagtgccgtg gcgtcggctt taccgaagcg ccgcgcggag cgttaggcca
    36001 ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg
    36061 gaacgccagc ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa
    36121 caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga
    36181 tccgtgcctc gcctgttcaa ctcatgtgct gggcgacgac ggtagcgagc tgatctccgt
    36241 gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag
    36301 ccactatgtc tttgaagcac cagttcgtat ctggcactgg ctgacggtgt tatgcatggc
    36361 ggtgttgatg gtcaccggtt actttatcgg caagccgcta ccttccgtca gtggcgaggc
    36421 gacgtatctg ttctatatgg gttacatcag gttaattcac ttcagcgccg ggatgatttt
    36481 taccgtggtc ttgctgatgc ggatctactg ggcttttgtt ggcaatcgtt actcccgcga
    36541 gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg
    36601 ctggtatctg tttctggcaa aacgtccgag tgctgatata ggccataacc ccatcgccca
    36661 ggcggcgatg ttcggctatt tcctgatgtc ggtctttatg atcatcaccg gttttgcgct
    36721 gtacagcgaa cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt
    36781 ctactggacg ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct
    36841 gattggggcg tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga
    36901 cgacacggtg atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag
    36961 taacaaggag cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg
    37021 tgggccgatg aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg
    37081 cccgagtatg tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc
    37141 gaaagcgcca gccatctgtt gattctcgat gccattgact acgggctgga acctggaagg
    37201 ctgcgaacct atgccggaga acgcattccg gcctatctca gcgcgaagaa aatgagcctg
    37261 catcagaaca gtttctccga agtgctggcg ctggcagata tccgcggaca tctgccagca
    37321 catattgccc tcgtcgggct gcaacccgcg atgctcgacg actacggcgg cagcctgagc
    37381 gaactggcac gggagcaact gcccgctgcg gaacaatcgg cgctggcgca gttggctgcg
    37441 tggggaagtg tgccgcaacc ggctaatgaa tcgcgttgtc tcaattatga ctgtctgtcg
    37501 atggaaaatt acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga
    37561 tgagcaacga cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc
    37621 cggtcagtga atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat
    37681 taagcagtga cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat
    37741 tactgcacga gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg
    37801 aagccatcgg cgatcgcttt ggcgcctttc gctttcctgc cactttagtg tttaccggcg
    37861 gaaactatcg cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc
    37921 gcgggcttgt cgaaccgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct
    37981 ggggccagga acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg
    38041 tcaggtgaat gatgaaccga gtatggcggc cttggagcaa tgtgctcaca gcccgcaggt
    38101 gattgcgctg ttaaacgagt tacaacatca actaagcgaa cgccagccgc cgttgggcga
    38161 ggtgctggca gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct
    38221 tctcggggaa ggggaagtgt cagtgcgcat tcagcaggct gacgacagta aaagtgaaat
    38281 acaggaggcg atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct
    38341 ggaggacaaa ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa
    38401 tctcttgccg acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc
    38461 gttggcgcat gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa
    38521 tctgacgcaa ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc
    38581 gggaaatatt cagattcgta ccattggcta tggcgagagc tatatcaact ccacggggtt
    38641 acgccatgtc tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta
    38701 tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc
    38761 gcagcggctt agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg
    38821 taagtatcgc tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt
    38881 tcaggataaa gagggagatc taacattatc gggttatttt tctctcttcg cctacaggag
    38941 tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc
    39001 actttttatt tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca
    39061 tttacgtggt caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct
    39121 tcggtatcaa ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta
    39181 ctaactggtc attctattcc aactatgtgg gcgatatttt tggcgcacca ctggcgatgg
    39241 aagcattaat ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc
    39301 aacggctgaa taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc
    39361 tctctgcatt gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt
    39421 ttgatatcga caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg
    39481 tcagccaggt gaaatttgtg cacaccgtca tggcgggcta cgtgaccggg gccatgttta
    39541 ttatggcgat cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgct
    39601 cgtttgccat cggttccgtc ttcggtactc tggcgattat cggtaccctg caactcggag
    39661 acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg
    39721 agtggcaaac ggaacctgct cctgcaccgt tccatgtggt tgcctggccg gaacaggatc
    39781 aagagcgtaa cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact
    39841 cattagataa acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc
    39901 aacgcggacg tatggcctgg ctgttaatgc aggaaatatc tcaaggcaat cgtgagccgc
    39961 atgtgttgca ggcattccgg gagctggaag gcgaccttgg ttacggcatg ttgctctccc
    40021 gctatgcgcc ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg
    40081 cgatacctca ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc
    40141 tgctgctact ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc
    40201 atcgctgggt gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag
    40261 ccgggtggtt tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga
    40321 catactccgc gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg
    40381 taggtcttta caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc
    40441 tggggccgag cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat
    40501 catgtttgat tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt
    40561 ggtctttatg atctccgacg gatttgacat ggggatcggc tgtttgctgc cgctggtggc
    40621 gcgtaatgat gatgaacgcc ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa
    40681 tcaggtctgg ttgatcctcg caggtggggc attatttgcc gcctggccca gagtgtatgc
    40741 agcggcgttt tccggctttt atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg
    40801 tccgctggcc tttgattatc gcggaaaaat tgccgatgca cgctggcgta aaatgtggga
    40861 tgccggtctg gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa
    40921 cttgttgctc ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atctcggcag
    40981 cttctggcaa ctgctgacgc cattcccttt attgtgtggt ttgctcagcc ttgggatggt
    41041 gattttgcaa ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc
    41101 acagctggcg actaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcaggtta
    41161 ctggctgtgg gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc
    41221 caatccgtta atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga
    41281 atcgcccgtt ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat
    41341 ggcgatttat cgtggtcgcc cgggttgggg atttttgatg gcatcattga tgcaatttgg
    41401 cgtgattttc acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gtgtgagtcc
    41461 aatctccagc ctgacgttgt gggacagcac ttccagtcag ctgacgctga gcattatgtt
    41521 ggtaatcgtg ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa
    41581 aatgtgggga cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg
    41641 agcaggaaca tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc
    41701 acccttgtgt tggtatggct ggacctgcgt ctgaaaagtt aacgaacgta ggcctgatgc
    41761 ggcgcattag catcgcatca ggcaatcaat aatgttagat atgaaaagcg gaaacatatc
    41821 gatgaaagcg atcttaatcc catttttatc tcttctgatt ccgttaaccc cgcaatctgc
    41881 attcgctcag agtgagccgg agctgaagct ggaaagtatg gtgattgtca gtcgtcatgg
    41941 tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc
    42001 aacttggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctatct
    42061 cggacattac caacgccagc gtctggtagc cgacggattg ctggcgaaaa agggctgccc
    42121 gcagtctgct caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga
    42181 agccttcgcc gccgggctgg cacctgattg tgcaataacc gtacataccc aggcagatac
    42241 gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataacgc
    42301 gaacgtgact gacgcgatcc tttgcagggc aggagggtca attgctgact ttaccgggca
    42361 tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat ttcccgcaat caaacttgtg
    42421 ccttaaccgt gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact
    42481 caaggtgagc gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac
    42541 ggagatattt ctcctgcaac aggcacaagg aatgccggag ccggggtggg gaaggatcac
    42601 cgattcacac cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttactaca
    42661 acgcacgccg gaggttgccc gcagtcgcgc caccccgtta ttggatttga tcatggcagc
    42721 gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct
    42781 gtttattgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg
    42841 gacgcttcca ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg
    42901 gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca
    42961 gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac
    43021 cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca
    43081 aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca
    43141 gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa
    43201 aaggcgttca cgccgcatcc ggtcactttc agttttactc tttctcggag taactataac
    43261 cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga
    43321 ttgcgccttt aatattaacg ccagcctgtt ccagacgctg cattgacaag ctcacctctt
    43381 tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccgacagag cgtcccacga
    43441 ccgcagcatc gctcaccgcc a
//
LOCUS       DADLZM010000017        42984 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLZM010000017 DADLZM010000000
VERSION     DADLZM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.017"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="JNY73_000934"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="JNY73_000934"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY8047029.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(1698..3305)
                     /locus_tag="JNY73_000935"
                     /pseudo
     CDS             complement(1698..3305)
                     /locus_tag="JNY73_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(3783..3869)
                     /locus_tag="JNY73_000936"
     tRNA            complement(3783..3869)
                     /locus_tag="JNY73_000936"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3833..3835),aa:Leu,seq:gag)
     gene            complement(3884..4216)
                     /gene="secG"
                     /locus_tag="JNY73_000937"
     CDS             complement(3884..4216)
                     /gene="secG"
                     /locus_tag="JNY73_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY8047030.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSTPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4444..5781)
                     /gene="glmM"
                     /locus_tag="JNY73_000938"
     CDS             complement(4444..5781)
                     /gene="glmM"
                     /locus_tag="JNY73_000938"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY8047031.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5774..6622)
                     /gene="folP"
                     /locus_tag="JNY73_000939"
     CDS             complement(5774..6622)
                     /gene="folP"
                     /locus_tag="JNY73_000939"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY8047032.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6712..8646)
                     /gene="ftsH"
                     /locus_tag="JNY73_000940"
     CDS             complement(6712..8646)
                     /gene="ftsH"
                     /locus_tag="JNY73_000940"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY8047033.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8746..9375)
                     /gene="rlmE"
                     /locus_tag="JNY73_000941"
     CDS             complement(8746..9375)
                     /gene="rlmE"
                     /locus_tag="JNY73_000941"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY8047034.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9501..9794
                     /gene="yhbY"
                     /locus_tag="JNY73_000942"
     CDS             9501..9794
                     /gene="yhbY"
                     /locus_tag="JNY73_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY8047035.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9950..10426)
                     /gene="greA"
                     /locus_tag="JNY73_000943"
     CDS             complement(9950..10426)
                     /gene="greA"
                     /locus_tag="JNY73_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY8047036.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10674..12107
                     /gene="dacB"
                     /locus_tag="JNY73_000944"
     CDS             10674..12107
                     /gene="dacB"
                     /locus_tag="JNY73_000944"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY8047037.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12293..13465)
                     /gene="cgtA"
                     /locus_tag="JNY73_000945"
     CDS             complement(12293..13465)
                     /gene="cgtA"
                     /locus_tag="JNY73_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY8047038.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13481..14446)
                     /locus_tag="JNY73_000946"
     CDS             complement(13481..14446)
                     /locus_tag="JNY73_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8047039.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLSPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14573..14830)
                     /gene="rpmA"
                     /locus_tag="JNY73_000947"
     CDS             complement(14573..14830)
                     /gene="rpmA"
                     /locus_tag="JNY73_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY8047040.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14851..15162)
                     /gene="rplU"
                     /locus_tag="JNY73_000948"
     CDS             complement(14851..15162)
                     /gene="rplU"
                     /locus_tag="JNY73_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY8047041.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15421..16392
                     /gene="ispB"
                     /locus_tag="JNY73_000949"
     CDS             15421..16392
                     /gene="ispB"
                     /locus_tag="JNY73_000949"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY8047042.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16620..16898
                     /gene="sfsB"
                     /locus_tag="JNY73_000950"
     CDS             16620..16898
                     /gene="sfsB"
                     /locus_tag="JNY73_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY8047043.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPRTHEFIDRTQLMRSYTKPKK"
     gene            complement(16946..18205)
                     /gene="murA"
                     /locus_tag="JNY73_000951"
     CDS             complement(16946..18205)
                     /gene="murA"
                     /locus_tag="JNY73_000951"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY8047044.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18260..18514)
                     /gene="ibaG"
                     /locus_tag="JNY73_000952"
     CDS             complement(18260..18514)
                     /gene="ibaG"
                     /locus_tag="JNY73_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY8047045.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18674..18967)
                     /gene="mlaB"
                     /locus_tag="JNY73_000953"
     CDS             complement(18674..18967)
                     /gene="mlaB"
                     /locus_tag="JNY73_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY8047046.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18967..19602)
                     /gene="mlaC"
                     /locus_tag="JNY73_000954"
     CDS             complement(18967..19602)
                     /gene="mlaC"
                     /locus_tag="JNY73_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY8047047.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19621..20172)
                     /gene="mlaD"
                     /locus_tag="JNY73_000955"
     CDS             complement(19621..20172)
                     /gene="mlaD"
                     /locus_tag="JNY73_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY8047048.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(20177..20959)
                     /gene="mlaE"
                     /locus_tag="JNY73_000956"
     CDS             complement(20177..20959)
                     /gene="mlaE"
                     /locus_tag="JNY73_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY8047049.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHVPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20967..21776)
                     /gene="mlaF"
                     /locus_tag="JNY73_000957"
     CDS             complement(20967..21776)
                     /gene="mlaF"
                     /locus_tag="JNY73_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY8047050.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21986..22951
                     /locus_tag="JNY73_000958"
     CDS             21986..22951
                     /locus_tag="JNY73_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY8047051.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLLLGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            22965..23951
                     /gene="kdsD"
                     /locus_tag="JNY73_000959"
     CDS             22965..23951
                     /gene="kdsD"
                     /locus_tag="JNY73_000959"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY8047052.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23972..24538
                     /gene="kdsC"
                     /locus_tag="JNY73_000960"
     CDS             23972..24538
                     /gene="kdsC"
                     /locus_tag="JNY73_000960"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY8047053.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24535..25110
                     /gene="lptC"
                     /locus_tag="JNY73_000961"
     CDS             24535..25110
                     /gene="lptC"
                     /locus_tag="JNY73_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY8047054.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            25079..25636
                     /gene="lptA"
                     /locus_tag="JNY73_000962"
     CDS             25079..25636
                     /gene="lptA"
                     /locus_tag="JNY73_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY8047055.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25643..26368
                     /gene="lptB"
                     /locus_tag="JNY73_000963"
     CDS             25643..26368
                     /gene="lptB"
                     /locus_tag="JNY73_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY8047056.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26416..27849
                     /gene="rpoN"
                     /locus_tag="JNY73_000964"
     CDS             26416..27849
                     /gene="rpoN"
                     /locus_tag="JNY73_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY8047057.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSRLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27872..28159
                     /gene="hpf"
                     /locus_tag="JNY73_000965"
     CDS             27872..28159
                     /gene="hpf"
                     /locus_tag="JNY73_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY8047058.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28277..28768
                     /gene="ptsN"
                     /locus_tag="JNY73_000966"
     CDS             28277..28768
                     /gene="ptsN"
                     /locus_tag="JNY73_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY8047059.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28814..29668
                     /gene="rapZ"
                     /locus_tag="JNY73_000967"
     CDS             28814..29668
                     /gene="rapZ"
                     /locus_tag="JNY73_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY8047060.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29665..29937
                     /gene="npr"
                     /locus_tag="JNY73_000968"
     CDS             29665..29937
                     /gene="npr"
                     /locus_tag="JNY73_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY8047061.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            30150..30782
                     /gene="yrbL"
                     /locus_tag="JNY73_000969"
     CDS             30150..30782
                     /gene="yrbL"
                     /locus_tag="JNY73_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY8047062.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30779..31507)
                     /gene="mtgA"
                     /locus_tag="JNY73_000970"
     CDS             complement(30779..31507)
                     /gene="mtgA"
                     /locus_tag="JNY73_000970"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY8047063.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31504..32157)
                     /gene="elbB"
                     /locus_tag="JNY73_000971"
     CDS             complement(31504..32157)
                     /gene="elbB"
                     /locus_tag="JNY73_000971"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY8047064.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32387..34723)
                     /gene="arcB"
                     /locus_tag="JNY73_000972"
     CDS             complement(32387..34723)
                     /gene="arcB"
                     /locus_tag="JNY73_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY8047065.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSHRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34819..35748)
                     /locus_tag="JNY73_000973"
     CDS             complement(34819..35748)
                     /locus_tag="JNY73_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY8047066.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36423..40883
                     /gene="gltB"
                     /locus_tag="JNY73_000974"
     CDS             36423..40883
                     /gene="gltB"
                     /locus_tag="JNY73_000974"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY8047067.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40896..42314
                     /locus_tag="JNY73_000975"
     CDS             40896..42314
                     /locus_tag="JNY73_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8047068.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42498..42977
                     /locus_tag="JNY73_000976"
     CDS             42498..42977
                     /locus_tag="JNY73_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY8047069.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        10705 a        11505 c        10588 g        10186 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt aaatactttt
      181 gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 aggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
     1021 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggttgcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc gtatcatcga
     1201 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
     1261 acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc tgatgctgcg
     1381 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
     1441 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta ttggtcaatt
     1561 gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt atgccaaaac
     1621 tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata tggaaaacaa
     1681 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct
     1741 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgt
     1801 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
     1861 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat attgttttcc
     1921 gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat cgggacatga
     1981 tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca
     2041 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
     2101 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
     2161 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
     2221 cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc
     2281 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcgcgc
     2341 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
     2401 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg
     2461 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
     2521 gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc aacttgcggt
     2581 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
     2641 atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata cggcaccgtg
     2701 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata gttgagattg
     2761 aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa cgctttttta
     2821 ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga aatgacaatc
     2881 agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt tattattact
     2941 gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata catccccagc
     3001 attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc atcattgaaa
     3061 gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc agtcagtaac
     3121 acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt aacaaccagg
     3181 atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga ggcgactaag
     3241 ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt aaaatcttct
     3301 agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata taaacaatta
     3361 tcaacaatgt tcaattttat atcagacatg tttttcaact ctgaaccata tcagaccgaa
     3421 acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta attaaataac
     3481 attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg
     3541 agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca
     3601 caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc gtaccggcac
     3661 catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa
     3721 ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc ttttctggaa
     3781 tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt
     3841 agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc
     3901 tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt gtactcagat
     3961 tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc accagactga
     4021 tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta ccagaaccac
     4081 ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt
     4141 taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacta
     4201 aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa
     4261 ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc
     4321 gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa
     4381 accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca gttatctaac
     4441 gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc
     4501 gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac gcagcaacac
     4561 gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa ctgactcatg
     4621 ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct gcgggaacat
     4681 tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg ccgccagcac
     4741 ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc
     4801 ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc
     4861 acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt caagccccat
     4921 gttgctcatc aatgtaccca cagcgccacc acgcagctgg ccctgacgaa gaccttcacg
     4981 cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat
     5041 cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg
     5101 agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgtttgg
     5161 ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg
     5221 ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc taagttcgtt
     5281 cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga tacggctggc
     5341 tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg cttcgatggc
     5401 ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa tcccattatc
     5461 gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt
     5521 cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc
     5581 cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc
     5641 aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt
     5701 aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc
     5761 gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca
     5821 cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc gcgccttgca
     5881 ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc cccacgttca
     5941 gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt
     6001 gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga
     6061 atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt
     6121 gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga gcttcctgca
     6181 tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg
     6241 cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga gcgccaactt
     6301 tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc
     6361 gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt
     6421 ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcattga
     6481 tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa
     6541 aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag
     6601 taccctgggc aaagagtttc atgatgttat ccctggtatg aattgataag aaaaaacccc
     6661 ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa cttacttgtc
     6721 gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg
     6781 aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc
     6841 cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt
     6901 ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag
     6961 ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg
     7021 tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga atacttcacc
     7081 ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat
     7141 gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc
     7201 cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc
     7261 cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag
     7321 cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg gcaccaggcg
     7381 accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg cttccgtcat
     7441 caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac
     7501 catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt
     7561 cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc
     7621 gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc
     7681 tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg ccgggtcgag
     7741 aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc
     7801 catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc
     7861 agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc
     7921 tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc
     7981 tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt
     8041 cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt
     8101 accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt cagcaacttc
     8161 ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt
     8221 cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat
     8281 ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa
     8341 gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt
     8401 caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact
     8461 atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt
     8521 attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc
     8581 aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt
     8641 cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat
     8701 actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc
     8761 ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct tacgaacttt
     8821 gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa
     8881 caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag
     8941 ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc
     9001 catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat
     9061 caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg
     9121 taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga
     9181 ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc
     9241 actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct ttttctgtgc
     9301 ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg
     9361 cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat
     9421 ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc
     9481 caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc
     9541 tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc
     9601 tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg
     9661 aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta
     9721 atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct
     9781 cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt
     9841 tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcagagcggc
     9901 ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt
     9961 ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat
    10021 cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc aggttttgtt
    10081 taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatccagat
    10141 tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat
    10201 caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct tcgcagaagc
    10261 cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg
    10321 cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt
    10381 cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt
    10441 gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc ccggcataac
    10501 tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac
    10561 aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg tttactttat
    10621 agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat
    10681 tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcgaatg
    10741 ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg gtgcaaaaag
    10801 tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta
    10861 cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat tttcgtttca
    10921 ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcat acttaagggt gacttagtgg
    10981 cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt
    11041 tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg
    11101 ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc
    11161 cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa
    11221 agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc
    11281 aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg gatgtggtgc
    11341 caggcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct gagccgctcc
    11401 cgttggcttt tggcgtgcag gatggagcca gctatgccgg tgcaattctg aaagatgagt
    11461 taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag gttaacgaac
    11521 ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt aagattatgc
    11581 tgaaaaagtc ggacaacatg attgccgata cggttttccg catgatcggc catgcgcgct
    11641 tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc ctgcgccagc
    11701 aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt tcgcggcata
    11761 acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg
    11821 aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg cagtaccgtg
    11881 caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt tcgttgcagg
    11941 gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcatttgtac
    12001 aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt attccgttag
    12061 tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca aaaagaaacc
    12121 ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat gccggatgcg
    12181 gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc aatcatcttg
    12241 taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt gattaacgct
    12301 tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat
    12361 catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac atgaattcga
    12421 ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg ataaaggtca
    12481 tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc agataatatt
    12541 tatcctccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct tctgccttat
    12601 ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga tcctggctgt
    12661 atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg
    12721 gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc aggaagcgaa
    12781 tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg gcaacaacga
    12841 agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg gtaaacggat
    12901 aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat ttacccgcgt
    12961 ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc agctcacgct
    13021 tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac gatttgaaac
    13081 gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga ccgtgtttag
    13141 tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct accggcactt
    13201 taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc
    13261 gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg ttctcgtcgg
    13321 cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg cctttcggaa
    13381 tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca acgaccagaa
    13441 tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct gcgcggggtt
    13501 gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca atggcggaat
    13561 acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt aacatcggtc
    13621 tggcgaagaa atcaggccag gccagtgata aaagatctga aaaaaacagc gtaaacagtg
    13681 gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca
    13741 aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg agctgccagt
    13801 ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt gtacataaag
    13861 tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc ttttgcgcca
    13921 cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc caggtgtaat
    13981 cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc agaccgctca
    14041 ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg ataaatacgc
    14101 tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca
    14161 ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg gtcgccactg
    14221 ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcgacaac ctcttcttca
    14281 ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac acgattgtcg
    14341 gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc cagcaaattg
    14401 ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg tattccctgc
    14461 aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa
    14521 aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa acttattcag
    14581 cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc actttaccgt
    14641 ctgctttggc aaacagagtg tggtcacgac cgcaacctac gttagcgcca gcgtggaatt
    14701 tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca ccgaaacgct
    14761 taacgcccag gcgtttagct tctgaatcgc gaccgttacg tgtggagccg ccagcctttt
    14821 tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc
    14881 agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac gaaacttaac
    14941 gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta cgccgccatc
    15001 aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca cttcagcgaa
    15061 ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct gaccttcgct
    15121 tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact ccgcttccgc
    15181 gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc gcgaatatta
    15241 cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa aaacacaact
    15301 tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat tcctaaccat
    15361 aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg
    15421 atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt taatgcggca
    15481 atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc
    15541 agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg agctgttggc
    15601 tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca cacggcgact
    15661 ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc taccgccaac
    15721 gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc
    15781 cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac
    15841 gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga catcactgaa
    15901 gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag
    15961 tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca ggattatggg
    16021 cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa tgccgatggc
    16081 gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg
    16141 ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac cgccatcgaa
    16201 caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc ttgtggatct
    16261 cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc agcgttacag
    16321 gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa
    16381 cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc cagtaaattc
    16441 cataaaaaca ctaattcagc gctataacac ctgaaaaaaa gggcgatgaa tctttatata
    16501 ttctgaatat tcacactctt tacaggaact ttttagcgca ataggccatc aggagtatag
    16561 tgatgcccga cagaagaagt gttctgaatg aaagcgacta acttaaggag tgaggaaaga
    16621 tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcaggtttg cgcaagaaag
    16681 gaacgtcaat ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg
    16741 cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg ggaactgacc
    16801 cctgggttat ctggccatca cgctaccatg atccgcggac ccatgagttt atcgacagaa
    16861 cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg tagccccgcg
    16921 aacggggctg ccagctctca gacgattact cgcctttcac acgctcaata tttgcaccta
    16981 aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa atacgatcaa
    17041 ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat gcacgcagat
    17101 cggttgccat aacctgtgca ccagaaagtt tttcaacacc atgacaaata acggtattgc
    17161 tttcgatttc ggcgtgcgcg cccatacggc tcagctctgg cacatgcata aagcggtttt
    17221 caaagaccgt ttcggtgatg aacccggtcc cttcagccac caggttcaac agcgtgaact
    17281 gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta acagccttcg
    17341 gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg tccgctccag
    17401 catcacgcag tttcgccagc acggcgtcca gagtatctgg ctgcgcgtta cggcagataa
    17461 ttttgccacg agagatcgcc gccgccacca ggaaagtacc ggtttcgata cgatccggca
    17521 gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata cgatcggtgc
    17581 cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg
    17641 gttcacgcgc tgcgttttca ataatcgtgg tgccttccgc cagggttgca gcacacatga
    17701 tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct ttcagacgac
    17761 catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct aattgttcga
    17821 ggccagaaat gtgtagatca accggacgag caccgatcgt acaaccgcca ggtagtgaaa
    17881 cttgcccctg accaaagcgc gctaccagcg gccccagcgc ccagatagaa gcacgcatgg
    17941 ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca atatgcacag
    18001 aaccattacg ttctactttc gcacccagct ggcttagcag tttcattgat gtatcgacgt
    18061 ctttcagttt cgggacgttc tggatctcta ccggttcttc cgccagtagt gcggcaaaaa
    18121 gaataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg
    18181 gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc atatccgcta
    18241 ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag
    18301 gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat tccatcagcg
    18361 ggccatagac cgtctgctgt tttttaaccc gactcatacc gtcaaacaac tcacccacgg
    18421 caataacctg aaagtgactg ccatcgccgg aaacgtggac ttcctggagg gagagagcgt
    18481 tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc agttaataaa
    18541 ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag cctcgctgat
    18601 aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa cgccatatcc
    18661 ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat ttcgcgaggg
    18721 tatacacttt gtcgtttacc ccctgaagcg tcacgttgtt gccctgcttt ttcgccagat
    18781 cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca cggctaagat
    18841 cgatgcaggt aatccccttc accgcttcct cacgcatttc ccaaagcggt agcagaacgt
    18901 cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag ctcagtgact
    18961 cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt tcagttgcgc
    19021 agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt gtttggtggt
    19081 gatcatactg acgccttcag caatcatgtc gtaagcttgc cagtttcccg tctgggagtt
    19141 tttacgccac tggaagtcca gacgcaccgg cggacggcca ttcgggtcaa taatggtaac
    19201 gcgaataggc acaatggttt tatcgcccag cggctgttct ggcgcaatct gataggtttg
    19261 accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac ggaaagcggc
    19321 aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca gcaccagcgc
    19381 accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg tacgcagata
    19441 atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg ttttctgcgc
    19501 cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg cactcagagg
    19561 tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa
    19621 ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag cagctggcgc
    19681 atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac caatgagatc
    19741 ttccagcacc atcgcagact tggtgtcctg aattgtatcg ccatccttca ggatagcagt
    19801 ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttctc ccagcaggcc
    19861 ggaagtacga atgctcagcg aactggtatc aggaatgtgg ttataacgtt gttcaatttc
    19921 cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca cccggcccac
    19981 aacaacacca ccaatgctga ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt
    20041 cgcataaagc gtgtaggtcg attcagtacg tatggacgtc acgttcgccg ccttcaggca
    20101 aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa tttcattttt
    20161 tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca
    20221 gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc ccggcagacg
    20281 tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg gcgaacacca
    20341 cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt tgcattgccg
    20401 accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagcgat ccgccccaga
    20461 tccccacggc gacgaagata accgtcaaca gtggtaatga aataacccca gcccagaaac
    20521 ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg gagagttgct
    20581 ctgtagcgcg catcaggccg atttctgcgg ttaacgccga accagcacgc ccggcaaaca
    20641 acagcgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc agcataccca
    20701 gactggtttc cgcgctataa gtggtcagaa ccagataacc ttgcagcccc aacaccattc
    20761 cgatgaacac gccagaaact acaataatca gcatcgacag gacgccgaca ttatagagct
    20821 ggcgcaccag cagcggcaca tgtttgcgaa attccggttt gccgaccagc gcattgaata
    20881 acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt ccgagcgacg
    20941 ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc gtgataatcg
    21001 ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg
    21061 cgcggatcag gattcgtctg caacgcctgg gcactgccat gagcgacaat ttttttgtcc
    21121 gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg agaaaccacc
    21181 acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag tacgcccatg
    21241 gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg ctccagcgca
    21301 atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga aggcattagt
    21361 ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact atgcaacaat
    21421 ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata
    21481 tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttacgcac tgtatacagg
    21541 cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc atgatctggt
    21601 gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga tggccccatg
    21661 atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat gcagcgattg
    21721 ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg ctccataatt
    21781 cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt gaacccaaca
    21841 tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt actttttagc
    21901 cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag cggaccggcg
    21961 agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa ttgttggttt
    22021 acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac
    22081 ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta catcgttacc
    22141 agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac
    22201 agccctcggc tcaaacatta tcaatatatt gctgctcctc ggtctggctg cgctggttcg
    22261 tccttttacc gtccattccg atgttctgcg ccgtgaatta cccttaatgt tgttggtcag
    22321 cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgaagcgatg gtatctttct
    22381 cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc aggggactga
    22441 cagcctgacc agagagcagc ttgcagagct gccgcgtgac ggcggattgc ccgtcgcgtt
    22501 tttatggctc ggcattgcgc ttatcatcat gccagtggcc acgcggatgg tggttgataa
    22561 cgccacggtg ctggcgaatt actttgccat cagcgagttg acgatgggtc tgacggcaat
    22621 tgctatcgga accagtctgc cggaactggc aaccgcaata gcgggggttc gcaaaggtga
    22681 aaacgacatt gctgtcggaa atatcattgg cgcaaacatt tttaatattg tcatcgtgtt
    22741 gggtttaccc gcgctgataa cgccaggaga gattgatcca ctggcgtaca gtcgtgacta
    22801 cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc gctccccgca
    22861 accgggccgt ggtgtagggg tattattaac tggcggattt atcatatggc tggcgatgtt
    22921 gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg cacgtagagt
    22981 tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat
    23041 gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcctgt gaaaagatgt
    23101 tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac atcgggcgca
    23161 aaatggccgc aacgtttgcc agcaccggta caccttcatt tttcgtccat cctagtgaag
    23221 ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt ggtgattgct atctctaact
    23281 caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt cacgtaccgt
    23341 taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat gtgcatctgt
    23401 gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc agcaccaccg
    23461 ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg
    23521 ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg
    23581 taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc
    23641 gtgacgcatt gctggaagtt acccgcaaaa atcttggtat gactgtcatt tgcgatgaca
    23701 atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg
    23761 tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc
    23821 ctggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc acctccgtga
    23881 tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta ctgcgtgcag
    23941 gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac
    24001 ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca gagaacattc gtctgctgat
    24061 cctcgacgtt gatggcgtac tgtcagatgg cctgatttat atgggcaata acggcgaaga
    24121 gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca cctctgatat
    24181 tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt gtgccacatt
    24241 ggggatcact cacttgtatc aggggcagtc aaacaaactg atcgccttta gcgatttgct
    24301 ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtt ggcgatgatc tcatcgactg
    24361 cccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat
    24421 cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc gcgaagtttg
    24481 cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat cgatatgagt
    24541 aaagccagac gttgggttat cattgtgcta tcactggcgg ttctggtgat gatcggcatt
    24601 aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga tcccacctat
    24661 aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag ctatcgattg
    24721 attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac gcagccggta
    24781 cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga taaagccaag
    24841 ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaacgc acttgtgcca
    24901 gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt gacgcaggat
    24961 gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag cggtctgaaa
    25021 atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag aacatcctat
    25081 gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac ttctggccgc
    25141 cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca ttgaatcgga
    25201 ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg tcatcgtcac
    25261 ccagggcacc atcaaaatta atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca
    25321 aggtaaagaa gtgattgacg gatacggtaa accggcaacg ttctaccaga tgcaggacaa
    25381 cggtaaaccc gttgaaggtc acgcttccca gatgcactac gaactggcaa aagattttgt
    25441 cgttctgacg ggtaatgctt atctgcagca ggtcgatagc aacattaagg gcgataagat
    25501 cacttacctg gtgaaagagc agaaaatgca ggctttcagc gacaaaggca agcgcgtaac
    25561 caccgttctg gtgccgtcgc agctgcagga caaaaacaac aaaggccaga ccccggcaca
    25621 gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg caaaagccta
    25681 taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg aaattgtcgg
    25741 tctgctgggg ccaaacggtg ccggtaagac caccactttc tacatggttg taggcattgt
    25801 gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc tgcctctgca
    25861 tgcacgcgcg cgccgcggta tcggctatct gccacaggaa gcctccattt tccgtcgcct
    25921 cagcgtttac gataacctga tggcggtact gcaaattcgt gacgacttgt ctgctgaaca
    25981 acgtgaagac cgcgcgaacg agctgatgga agagtttcac attgagcacc tgcgtgacag
    26041 catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc
    26101 tgcgaatccg aaatttattc tgctcgacga accgtttgcc ggggttgacc cgatctcggt
    26161 tatcgatatt aaacgcatca ttgaacacct gcgcgacagc ggcctgggcg tgctgatcac
    26221 tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg
    26281 gcatttgatc gcccacggca cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt
    26341 ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt ttagcaggag
    26401 agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca actggcgatg
    26461 acgccacagc tccaacaggc aattcgtctg ttgcagttgt cgaggttgga acttcagcag
    26521 gagctacagc aggcgctgga gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa
    26581 atcgacaccc gcgaaacgca agacagtgaa acgctggaca ccgccgacgc gctcgaacaa
    26641 aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta caccgctggt
    26701 acaccatccg gcaccagcgg tgactacatt gacgacgagc tgccggtcta ccagggcgaa
    26761 acgacgcaga ccttgcagga ttacctgatg tggcaggtcg agctgacacc gttttccgac
    26821 actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgaaaccgg ttatctgact
    26881 gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt
    26941 gaagccgtcc ttaagcggat ccaacggttt gatccggtcg gtgtagcggc aaaagatctg
    27001 cgtgactgcc tgctaatcca actctcccaa ttcgataaga ccacgccgtg gctggaagag
    27061 gccagattga tcattagcga tcatctcgat ctgttagcca atcacgactt ccgcacttta
    27121 atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg
    27181 ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt cattccagat
    27241 gtgctggtgc gtaagcataa cggtcactgg acggtagaac tcaacagtga cagcattccg
    27301 cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac
    27361 agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag tctggaaagc
    27421 cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca agccttcttt
    27481 gagcaaggtg aagaatatat gaaaccgatg gtactggccg atatcgccca ggctgtcgaa
    27541 atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc
    27601 atttttgaac tgaagtattt cttttccagt cacgtcaata ccgagggcgg cggcgaagct
    27661 tcctccacgg cgattcgtgc actggtgaag aaattaatcg cggcggaaaa cccagcgaaa
    27721 ccgttgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat ggtggcacgc
    27781 cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca gcgtaaacag
    27841 ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg gaaataacgt
    27901 cgagatcacc gaggcactgc gcgaatttgt tacagctaaa tttgccaaac ttgagcaata
    27961 ttttgaccga atcaaccagg tctatgttgt tctgaaagtg gagaaagtca cccacacctc
    28021 agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag gtcaggacat
    28081 gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca aacataaaga
    28141 taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc ctgttgtgcg
    28201 gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt gagtgggcag
    28261 gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca gtgttcttaa
    28321 cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat
    28381 cagcgagctg gcggcgaaac aacttagcct accgcctcag gtggtttttg aagctatcct
    28441 gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc cgcatggcaa
    28501 acttgaagaa gatactctgc gtgccgttgg cgttttcgtt cagctcgaaa cgcctatagc
    28561 tttcgatgcc atcgacaacc aaccggtgga cttacttttt gccctgctgg tgccggcaga
    28621 ccaaactaaa acgcacctgc atactctgtc gctggtggcg aaacgtctgg cggacaaaac
    28681 catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa tcattacgga
    28741 taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta gacgttgtga
    28801 ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt
    28861 cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc ccgtagtgtt
    28921 gttacccgat ctggctcgaa ctctggccga tcgcgagatt tctgccgccg tcagcattga
    28981 tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta acctgcctga
    29041 cgctttctca ccgcaactac tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta
    29101 cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat
    29161 cgacaaagaa agcgatttgc tggagcctct gcgttcgcga gcggatctga ttgtcgatac
    29221 ctcagaaatg tccgttcacg agctggctga aatgctgcgt acccgtctgc tgggtaaacg
    29281 tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc aaacacggta tccctatcga
    29341 tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc cgaaactgcg
    29401 tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca
    29461 caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc tggaaaccaa
    29521 caaccgtagc tacctgacgg tcgccattgg ttgtaccggc gggaagcacc gttcggtgta
    29581 tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca
    29641 tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag
    29701 ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct
    29761 gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt tattgcgctg
    29821 ctgatgttgg attctgccaa aggacggcag attgaagttg aagcgaccgg tccacaggaa
    29881 gaggaagcac tggccgccgt tatcgccctc tttaattctg gttttgatga agattaatct
    29941 tcatcacttt gacatacaac tcccttcaag ctcccccgac aataagaaaa tcacgtactg
    30001 aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc
    30061 ttaagaaaac cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag
    30121 atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg
    30181 gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga
    30241 ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg
    30301 cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg
    30361 agacggattg cggaaccggt tatgtctacg atgtgattgc cgattttgac ggcaagcctt
    30421 ccattacgct gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc
    30481 gccagctact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc
    30541 tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct
    30601 gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc
    30661 tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc
    30721 tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt
    30781 aatccagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa
    30841 tccacgcctg acggctacgc acgtagccgt caggcgtgga gactttgaaa cgaagtggat
    30901 taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt
    30961 ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat
    31021 tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc
    31081 cagcttccag cccttttcgt acccagctac gcccatccca taaaaagaga tttttggccg
    31141 tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca
    31201 gggctttctc aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg
    31261 cggcaatcac cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat
    31321 gtgccacgta acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg
    31381 ctgagaaggg aacaggcgca acgctaaaca acgcgatgcc cccgccccag aaaacggcga
    31441 ggacaaccat taaccgcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt
    31501 tactcattca gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc
    31561 tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac
    31621 cacgatatca tcgacaggac acggcacatg ctccgcgccc atctcttcca gtacttctgc
    31681 ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat
    31741 cgccggggca atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag
    31801 cgcctttaat tcacggtcaa cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt
    31861 cttcgccgcg ccaacccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg
    31921 ggccagagga cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt
    31981 ttccgtcatc gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc
    32041 aaagcagacc gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg
    32101 aatttcagaa ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt
    32161 gatcatcctt tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc
    32221 acacatttaa ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg
    32281 cctgaaaaac agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat
    32341 ttccctggtg ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg
    32401 cttttgccac ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt
    32461 caatccattc accgacgtta tcttcccagg cgggaaggtc aggagactga atttgctgac
    32521 ccagctgttg cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt
    32581 cctcaacaat gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc
    32641 ccggcatcat cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt
    32701 cgagatactg ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg
    32761 tcgtcaccgt actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta
    32821 gcgccggaac cgaaagcggc ttactcagca catcatccat tccagcattg aggtactctt
    32881 gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat
    32941 aacgcttcgt cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca
    33001 gcaacaccag gtcgtattcg cccggtttaa acatctccag cgccgccttg ccggtcatgg
    33061 cgacatcaac gctgttacct aatttttcca gcacagaacg cgcgacaatc acgttcagtt
    33121 caatgtcttc caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg
    33181 catcatcgac ctcttctgcc accgacggtg cgtggatcgt caacgtaaag gttgaacctt
    33241 tgccctgttc gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgatgagaaa
    33301 cggccagacc aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt
    33361 aatacatggc gaagatttta tccagctcat cctgcggaat gccgatacca gaatcttcca
    33421 cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct
    33481 gggtgaattt gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt
    33541 cggtaatgac ctgatgcggt aatggcagcg taggctccag gttaaagcgc aatccttttt
    33601 gttgcgcctg caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg
    33661 gctgattatc aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa
    33721 agatattccc cagcgtgacg gccgaaacat ggatagtttt gagatatttt tcctgctcgg
    33781 cggtgagttc ggtatccagc agaatgcggc tcaggccgac gataccgttc agcggtgtac
    33841 gcaattcgtg actgatggtg gaaataaacg tcgttttgtc gcggctggcg cgttcaagcg
    33901 catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt
    33961 tacccacgcg gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg
    34021 ggtaatccag ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg
    34081 tttcaatgac ttttgcggcg gcttccggtg agtaaacatc agcaggtttc agatgaacca
    34141 gttgtttttc gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt
    34201 tatcttcgtt acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg
    34261 aggattgctg ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt
    34321 ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg aacggcaatt tcctgattta
    34381 gctgggcaat attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct
    34441 ccagtttttg caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca
    34501 ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata gaacgaataa
    34561 catcaatgct ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg
    34621 caagaacgac gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca
    34681 tcaggtcaac ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg
    34741 acaacctgcc ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc
    34801 ggaattcccc tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc
    34861 ctgtacgcca tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc
    34921 gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat
    34981 cacttccggt ggcgtatggc gaatcatttc tccggcagtg agcgtataat cctccagttc
    35041 aataccgttc aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg
    35101 cagcggatgc agctttatgc cgtccacgct ggtttcaacc actcgctcca gcgtttgcaa
    35161 acattcagcc tgcccttcgc caggcaggcc gacaattaag tgggaacaca ccttcagccc
    35221 gcgctgacgt gccagctggg ttgtacgctg ataacaggca aaatcatggc cgcggttgat
    35281 gcgatgcagt gttttgtcgt gggcggtttg tagccccagc tccagccaca cttcgtaacc
    35341 ctggtcctta tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgggt
    35401 accaacacac aaaccgacaa tattggcctg gctcaccgcc tgctgataca tagaacgcag
    35461 cacctgaact tccgcaaagg tgctggtata cgcctgaaag taggccagat agcgtttagc
    35521 gcggttaact aaattcgcct ggtgcgccag ttgctcggca atggaacgat gctgctgcgc
    35581 ttcatcggca aacgaggcaa cattacagaa tgtgcagcca ccacgcccga tggtaccgtc
    35641 acggttaggg cagctaaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg
    35701 acgggtgaga tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac
    35761 cgcgccttga aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctagat
    35821 caatcgtccc ggcctgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg
    35881 cataatcaca taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat
    35941 tttaggcaaa aacagtagca tgaaacgtca ttaccaatta aggcagtata aaatgctggt
    36001 tttctcgtca gttcaaggca ggataagggt taacacgcct ttatgacagt caggaattga
    36061 ctgtttctct aacgactctc cttttagcct taaagataaa atctatttta atttcagcca
    36121 tttaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct
    36181 gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct
    36241 catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga
    36301 caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt
    36361 ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg gaggttcacg
    36421 atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca
    36481 tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc
    36541 agcaccgtgg cgcgattctc gccgatggta aaaccggcga cggttgcggc ttgctgttac
    36601 aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa
    36661 actacgctgt cgggatgctc ttcctgaata aagatcctga actcgccgct gccgcacgcc
    36721 gcatcgttga agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc
    36781 ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa
    36841 tttttgtgaa cgccccggca ggctggcgtc cacgcgatat ggagcgccgt ctgtttatcg
    36901 cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt
    36961 cgaatctggt gaacatctat aaaggtctgt gtatgccggc ggatctgccg cgcttttatc
    37021 tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca
    37081 ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg
    37141 aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga
    37201 cgccgctgat ccctgacctg cacgacgccg cgccattcgt caacgaaacc ggttctgact
    37261 ccagttccat ggataacatg ctggaactgc tgctggcagg cgggatggat atcatccgcg
    37321 ccatgcgcct gttagtgcca cccgcctggc agaacaaccc ggatatggac ccggaactgc
    37381 gtgccttctt tgactttaac tccatgcata tggagccgtg ggatggtccg gcgggcatcg
    37441 tgatgtccga tggtcgcttc gctgcctgta acctcgaccg taacggtctg cgtccggcac
    37501 gctacgtcat taccaaagac aagctcatca cctgcgcctc tgaagtcggt atctgggatt
    37561 accagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggtgatcg
    37621 acactcgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc
    37681 acccttataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc
    37741 ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac
    37801 agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc
    37861 aggaagcggt cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc
    37921 gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg ccaatcgacc
    37981 cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct
    38041 tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact
    38101 ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata
    38161 tcacctttga cgtcactaaa accacgctcg aagcgacagt caaagagcta tgcgacaaag
    38221 ccgaaaaaat ggtacgtagc ggcaccgtgt tgctggtgct ctccgaccgg aatatcgcta
    38281 aagatcgcct gccggttccg gctccgatgg cggttggcgc gatccagacc cgtctggtcg
    38341 atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc
    38401 accacttcgc cgtgttgctg ggtttcggcg cgacggctat ttatccgtac cttgcctatg
    38461 aaacgctggg ccgcctggta gacacccacg cgattgccaa agattatcgt accgtgatgc
    38521 tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct
    38581 ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg
    38641 tagtgggcct gtgcttccag ggcgcggtca gccgcattgg tggggcaagc tttgaagact
    38701 tccagcagga tctgctgaat ctgtcgaaac gtgcctggct ggcgcgcaag ccgattagcc
    38761 agggcggcct gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg
    38821 tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga
    38881 agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg
    38941 aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa cgctttgata
    39001 ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga
    39061 tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg cgaagacccg gcgcgctatg
    39121 gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg tcgcttcggc gttactccgg
    39181 cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg
    39241 aaggcggtca gttgccgggc gacaaagtca ctccttacat cgccaaactg cgctattcgg
    39301 tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaagact
    39361 tagcgcagct tattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgttaagc
    39421 tggtgtccga accaggcgtt ggcaccatcg cgactggcgt ggcgaaagct tatgcggact
    39481 tgatcaccat cgcgggctat gacggcggca ccggcgcaag cccgctgtcg tcagtgaaat
    39541 atgcaggctg cccgtgggaa ctcggtctgg tggaaaccca acaggcgctg gttgccaacg
    39601 gcttgcgtca taaaattcgt ttgcaggttg atggcggcct gaaaacgggt gtcgatatca
    39661 tcaaagcggc gattctcggc gcagaaagct tcggcttcgg cactggccca atggtggcgc
    39721 tcggctgtaa atatctgcgt atttgccacc tgaacaactg cgccacgggt gtagcaactc
    39781 aggatgacaa actgcgtaag aatcactatc acggcctgcc gttcaaggtg actaattact
    39841 ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca gctgggagta acgcgtctgg
    39901 tggatctgat tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc
    39961 agaaactggc gctgtcgaag ctgctggaga ctgccgagcc acatgcaggt aagtcactct
    40021 actgcactga aaacaacccg ccgtttgata acggcctact gaacgcgcag ttgctgcaac
    40081 aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat atccgcaaca
    40141 ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg
    40201 ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg
    40261 tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga tgccaacgac tatgtcggta
    40321 aaggcatggc gggcggctta atcgccattc atcctccggt tggttccgcc ttccgcagcc
    40381 atgaagcaag cattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg
    40441 ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag
    40501 aaggcattgg cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta
    40561 aaaccggcgt taacttcggc gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa
    40621 gcggtgattt ccgcaaacgc gttaacccgg aactggtcga agtcttaagc gtcgacgatc
    40681 tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag cataccggtt
    40741 ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccacgaaa ttcgcgctgg
    40801 ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt agcgcagcag
    40861 agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta
    40921 tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgtaaa attgagtttg
    40981 ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgtctgt
    41041 cgtgcggcaa cccatactgc gagtggaaat gcccggtaca caactacatc ccaaactggc
    41101 tgaagctcgc caacgagggg cgtatttttg aagcggcaga actgtcgcat cagaccaaca
    41161 cactgccgga agtatgcgga cgtgtctgcc cgcaagaccg tctgtgcgaa ggttcctgca
    41221 ccctgaacga tgagttcggt gcggtgacca tcggcaacat tgagcgttat atcaatgata
    41281 aagcgttcga aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag
    41341 tggcgattat cggtgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg
    41401 gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta
    41461 ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca
    41521 tgggtattga attcaaactc aataccgaag tgggccgcga cgtacagctg gacgatctgc
    41581 tgagtgatta cgatgccgtg ttccttggcg ttgggactta tcagtcaatg cgcggcgggc
    41641 tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca
    41701 aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac
    41761 gcgtggtagt acttggcggt ggcgacactg cgatggactg cgtgcgcacg tctgtacgcc
    41821 agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc
    41881 gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac
    41941 tggggattga agtgaacggt aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa
    42001 tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata
    42061 tcgttccggc agatgcggtg atcatggcgt ttggtttccg tccacacaac atggaatggc
    42121 tggcaaaaca cagcgtcgag ctggattcac aaggccgcat catcgctccg gaaggcaacg
    42181 acaacgcttt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt
    42241 ccgatctggt cgttaccgcc atcgccgaag gtcgcaaagc ggcagacggc attatgaact
    42301 ggctggaagt ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat
    42361 tctcctttct ttctggaaat cgcctcgcat aatctgaaga aaaccaatgt aatacgctca
    42421 tctggattgt taccggtaga tgcccggcgt atagctgatt ccattattca acgcagcgcc
    42481 atataaaggg acagataatg gaatcgctct cagagagaac ctcaacaggc taccagcaaa
    42541 tccacgacgg cattattcat ctggtcgata gcgcccgaac ggaaacggta cgtagcgtta
    42601 acgcgctgat gaccgcgacg tactgggaaa ttggccgacg gattgtcgaa tttgaacaag
    42661 gtggtgaggc cagagcagct tatggtgcac agttgattga tcggctatcg aaggatttaa
    42721 gtcgacggta taaacgtggg gtctctgcaa aaaacttacg ccaaatgagg cttttttacc
    42781 tcttttttca acatgttgaa attcgccaga cagtgtctgg cgaattaaca ccattgggaa
    42841 ttccgcagac accatctgca gaattcccat cggcaaagat ttggcagacg ctgtctgcca
    42901 aattgagagg ctgtttgata tttccacagc ttttgtattg catctcctat tttcggtttg
    42961 tatattcatg caaataaacg aagg
//
LOCUS       DADLZM010000018        42264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLZM010000018 DADLZM010000000
VERSION     DADLZM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.018"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(164..955)
                     /gene="cpoB"
                     /locus_tag="JNY73_000977"
     CDS             complement(164..955)
                     /gene="cpoB"
                     /locus_tag="JNY73_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY8047070.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAHAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(965..1486)
                     /gene="pal"
                     /locus_tag="JNY73_000978"
     CDS             complement(965..1486)
                     /gene="pal"
                     /locus_tag="JNY73_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY8047071.1"
                     /translation="MQLNKVLKGLMIALSVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="JNY73_000979"
     CDS             complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="JNY73_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY8047072.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2946..4187)
                     /gene="tolA"
                     /locus_tag="JNY73_000980"
     CDS             complement(2946..4187)
                     /gene="tolA"
                     /locus_tag="JNY73_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY8047073.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4252..4680)
                     /gene="tolR"
                     /locus_tag="JNY73_000981"
     CDS             complement(4252..4680)
                     /gene="tolR"
                     /locus_tag="JNY73_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY8047074.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4684..5376)
                     /gene="tolQ"
                     /locus_tag="JNY73_000982"
     CDS             complement(4684..5376)
                     /gene="tolQ"
                     /locus_tag="JNY73_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY8047075.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5373..5777)
                     /gene="ybgC"
                     /locus_tag="JNY73_000983"
     CDS             complement(5373..5777)
                     /gene="ybgC"
                     /locus_tag="JNY73_000983"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY8047076.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5927..6220)
                     /gene="ybgE"
                     /locus_tag="JNY73_000984"
     CDS             complement(5927..6220)
                     /gene="ybgE"
                     /locus_tag="JNY73_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY8047077.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6220..6333)
                     /gene="cydX"
                     /locus_tag="JNY73_000985"
     CDS             complement(6220..6333)
                     /gene="cydX"
                     /locus_tag="JNY73_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY8047078.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6348..7487)
                     /gene="cydB"
                     /locus_tag="JNY73_000986"
     CDS             complement(6348..7487)
                     /gene="cydB"
                     /locus_tag="JNY73_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY8047079.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7503..9071)
                     /gene="cydA"
                     /locus_tag="JNY73_000987"
     CDS             complement(7503..9071)
                     /gene="cydA"
                     /locus_tag="JNY73_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY8047080.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9920..12589)
                     /gene="mngB"
                     /locus_tag="JNY73_000988"
     CDS             complement(9920..12589)
                     /gene="mngB"
                     /locus_tag="JNY73_000988"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY8047081.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLEPLEPLATLAWTLGFEYHH
                     GLLEKIWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMP
                     QSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLID
                     RQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQ
                     IALNEDGTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEI
                     IHEAWQSRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQA
                     DDHRLRVLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYA
                     ALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMP
                     VPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFN
                     VPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSET
                     MMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12607..14583)
                     /gene="mngA"
                     /locus_tag="JNY73_000989"
     CDS             complement(12607..14583)
                     /gene="mngA"
                     /locus_tag="JNY73_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY8047082.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWESVDGPEAVDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNALSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMQ"
     gene            14693..15415
                     /gene="mngR"
                     /locus_tag="JNY73_000990"
     CDS             14693..15415
                     /gene="mngR"
                     /locus_tag="JNY73_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY8047083.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15519..16388)
                     /gene="sucD"
                     /locus_tag="JNY73_000991"
     CDS             complement(15519..16388)
                     /gene="sucD"
                     /locus_tag="JNY73_000991"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY8047084.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16388..17554)
                     /gene="sucC"
                     /locus_tag="JNY73_000992"
     CDS             complement(16388..17554)
                     /gene="sucC"
                     /locus_tag="JNY73_000992"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY8047085.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17648..18865)
                     /gene="odhB"
                     /locus_tag="JNY73_000993"
     CDS             complement(17648..18865)
                     /gene="odhB"
                     /locus_tag="JNY73_000993"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY8047086.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(18880..21681)
                     /gene="sucA"
                     /locus_tag="JNY73_000994"
     CDS             complement(18880..21681)
                     /gene="sucA"
                     /locus_tag="JNY73_000994"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY8047087.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(21982..22698)
                     /locus_tag="JNY73_000995"
     CDS             complement(21982..22698)
                     /locus_tag="JNY73_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY8047088.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22714..24480)
                     /gene="sdhA"
                     /locus_tag="JNY73_000996"
     CDS             complement(22714..24480)
                     /gene="sdhA"
                     /locus_tag="JNY73_000996"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY8047089.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24480..24827)
                     /gene="sdhD"
                     /locus_tag="JNY73_000997"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24480..24827)
                     /gene="sdhD"
                     /locus_tag="JNY73_000997"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY8047090.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24821..25225)
                     /gene="sdhC"
                     /locus_tag="JNY73_000998"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24821..25225)
                     /gene="sdhC"
                     /locus_tag="JNY73_000998"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY8047091.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25606..25713
                     /locus_tag="JNY73_000999"
     CDS             25606..25713
                     /locus_tag="JNY73_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047092.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25919..27202
                     /gene="gltA"
                     /locus_tag="JNY73_001000"
     CDS             25919..27202
                     /gene="gltA"
                     /locus_tag="JNY73_001000"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY8047093.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27593..28159
                     /locus_tag="JNY73_001001"
     CDS             27593..28159
                     /locus_tag="JNY73_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8047094.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28210..30669
                     /locus_tag="JNY73_001002"
     CDS             28210..30669
                     /locus_tag="JNY73_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8047095.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQY
                     CSLHLPEHMEDTANRLILPCH"
     gene            30684..31415
                     /locus_tag="JNY73_001003"
     CDS             30684..31415
                     /locus_tag="JNY73_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8047096.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31412..32473
                     /locus_tag="JNY73_001004"
     CDS             31412..32473
                     /locus_tag="JNY73_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8047097.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIHVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            32580..33671
                     /locus_tag="JNY73_001005"
     CDS             32580..33671
                     /locus_tag="JNY73_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY8047098.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33668..34459)
                     /gene="nei"
                     /locus_tag="JNY73_001006"
     CDS             complement(33668..34459)
                     /gene="nei"
                     /locus_tag="JNY73_001006"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY8047099.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34495..35229)
                     /gene="pxpA"
                     /locus_tag="JNY73_001007"
     CDS             complement(34495..35229)
                     /gene="pxpA"
                     /locus_tag="JNY73_001007"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY8047100.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKETQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35219..36151)
                     /gene="pxpC"
                     /locus_tag="JNY73_001008"
     CDS             complement(35219..36151)
                     /gene="pxpC"
                     /locus_tag="JNY73_001008"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY8047101.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(36145..36801)
                     /gene="pxpB"
                     /locus_tag="JNY73_001009"
     CDS             complement(36145..36801)
                     /gene="pxpB"
                     /locus_tag="JNY73_001009"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY8047102.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36824..37567)
                     /gene="ybgI"
                     /locus_tag="JNY73_001010"
     CDS             complement(36824..37567)
                     /gene="ybgI"
                     /locus_tag="JNY73_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY8047103.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLENDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37838..39319
                     /gene="dtpD"
                     /locus_tag="JNY73_001011"
     CDS             37838..39319
                     /gene="dtpD"
                     /locus_tag="JNY73_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY8047104.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39469..40887)
                     /gene="phrB"
                     /locus_tag="JNY73_001012"
     CDS             complement(39469..40887)
                     /gene="phrB"
                     /locus_tag="JNY73_001012"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY8047105.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(40884..41393)
                     /locus_tag="JNY73_001013"
     CDS             complement(40884..41393)
                     /locus_tag="JNY73_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY8047106.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41522..41839
                     /locus_tag="JNY73_001014"
     CDS             41522..41839
                     /locus_tag="JNY73_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY8047107.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGMARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(41894..42214)
                     /locus_tag="JNY73_001015"
     CDS             complement(41894..42214)
                     /locus_tag="JNY73_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047108.1"
                     /translation="MPGYRTWIEISLIDELKPSYPFRLEEYAMIGHSPYANECNKDEA
                     KFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELKTRLSTLHVNLEQFINSSEDD
                     EYTL"
BASE COUNT        10284 a        11330 c        10255 g        10395 t
ORIGIN      
        1 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
       61 ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta tgattcgcac
      121 gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc gcgttcagac
      181 gtttttgtgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      241 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact ttaaacatcg
      301 catcagcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa tagtacgccg
      361 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      421 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      481 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcgccgc
      541 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
      601 ccgcaccgct ttgatcgctg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
      661 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
      721 cctgaatctg accacgcagg gaatcaatat cggattgatt atcagaaagt tgttgctgga
      781 gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg acgcggtctt
      841 cgaccgagcc tgagccgaca ctactgattg gtgcatgagc aaaagcggcc cagggggccg
      901 ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg ctcatgcaat
      961 tctcttagta aaccagtacc gcacgacggt ttttggagta tgctgcttcg tcatgaccca
     1021 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt
     1081 taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag atgttgtatt
     1141 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1201 gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1261 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1321 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgcca gcacccagca
     1381 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1441 cagacagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1501 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1561 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     1621 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     1681 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     1741 atgtgctgct gcccaccatt ggagctgacc attaccataa atttaccgtc gctgctgaca
     1801 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     1861 ttcactttat aaacctgcgg acgaccggcc tggtcagaag taaatgccag gttctggcta
     1921 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     1981 ccagaagcca aatccattac gtacaggttc agactaccgg ttttcgacaa ggcgaatgcc
     2041 agtttgctgc cgtctggcga gaatgcaggt gcaccgttgt gacgcgggaa tgaagccacc
     2101 tgacgtacag cgccatttgc cagcgtctga ataaccagcg cggaacgacc gctttcgaag
     2161 gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag cggctgcggt
     2221 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2281 aactgaccgc cgttggtctg aacaacgtag gcaatacggg tacggaacgc acctttaatg
     2341 ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcataacg cagccactgc
     2401 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2461 agttgataag caacattgta agaaccatcc ggattcggag tgacctgacc gacaactaca
     2521 gcgtcaatgc ccagtgcgga ccatgcagct ggttgtactt cctgcgcact acccggctgc
     2581 tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa gtcagcagca
     2641 acgatgccgc caatatcttc aggtgccgca ccaggccccg cccactggaa aggaacaaca
     2701 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     2761 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     2821 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     2881 aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtccgaa cagtcaacat
     2941 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3001 ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca aagtgcggga
     3061 tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt tatgcgcagc
     3121 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3181 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3241 ctcccggcag gcgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3301 ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc tgcagcggct
     3361 ttttcagctg ctgcttttgc cgctgccgct tttttgtctg cggcggcttt ttctgccgct
     3421 gctttcttat cagctgctgc cttatcagca gccgcttttt ctgctgcttt tttgtcggct
     3481 gcagctttct ctgctgccgc tttcttatca gctgcagcct tttcagcagc cgctttcttc
     3541 tcagcttctg ccttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct
     3601 gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg cagcggctac ctcggctttt
     3661 ttctgcgctt cggctgcggc tttggcggct tctgcttctg cttttttctt cgcgtctgca
     3721 gccgctttct tcgcagcttc ttccgcagct ttagcatctg ccgccgcttt cgctgccgcc
     3781 acttcagctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc
     3841 tgctcctgag ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct
     3901 tgtttctcac gcagttcttc agcagcctgc tgttccttca tcttgcgctg ttcatcagaa
     3961 cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa
     4021 tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc
     4081 tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata
     4141 attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa
     4201 aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg
     4261 ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat
     4321 tatttcatcg taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc
     4381 cttgaaacgg ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc
     4441 tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt
     4501 atcgttactg ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg
     4561 ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta cgtccagcaa
     4621 cggtacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat
     4681 ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa
     4741 ctcttccata aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgagacggtt
     4801 ataagccatt accgccggaa ttgcggcgaa cagaccaatt gccgtcgcaa tcaacgcttc
     4861 tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa
     4921 ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac
     4981 cgtaccgagg aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg
     5041 catagcacgc gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag
     5101 gcgcacaaac tctttgaacc cgctgtaaaa gatttgttcc gaaccagtca gattatcccg
     5161 tttcccctga ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc
     5221 aaacgcttcg gcttcacgcg ccgctgcgtt aagaatacgg gttcgctgga taataatggc
     5281 ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc
     5341 cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac
     5401 ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct
     5461 tcattcagca aagtattctc ggcgttgaca atatgttgcg tgaaaaccaa agaggtgcca
     5521 cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa
     5581 tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga
     5641 ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg
     5701 gcgtggtaca ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga
     5761 aacagcgttg tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc
     5821 tgctactata cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg agcgtaaaaa
     5881 tttatgggcc tggagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga
     5941 aaatcagccc gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa
     6001 gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca
     6061 gcaataaacc atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc
     6121 aaaacataca tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct
     6181 tgtccattac cgcatacaag gtcgcgataa tcttactcat cagatgtctt cttgaccggc
     6241 tttgcctgat tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag
     6301 aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta gtacagagag
     6361 tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag
     6421 gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc
     6481 gtcagctggc tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac
     6541 ggcatcacaa acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg
     6601 gaggagaaca caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc
     6661 ggcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc
     6721 agccatgcgc cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg
     6781 tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt
     6841 gcgaaacaga ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc
     6901 agttcgcccc cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg
     6961 ctcaccacgc ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag
     7021 tacagacgca gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct
     7081 acaccaatta ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta
     7141 cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa
     7201 gacgccagca cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc
     7261 ggccaggcag caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag
     7321 tgtggtgcaa tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg
     7381 gtgagcatgc ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg
     7441 ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg
     7501 tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc
     7561 aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc caccaggaac
     7621 agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac
     7681 gaattcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca
     7741 gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg
     7801 cgcagaagcc attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg
     7861 atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcaaa gtacagcggc
     7921 gctacacgcg ggatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc
     7981 acgtttggcg tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg
     8041 aactggtcac gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac
     8101 gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata
     8161 accggggtat ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca
     8221 aatttgttcg tctcctcttc ctgatcagga atgccgaaca aagtaaaggc agcaggcgca
     8281 ggttgcgttt cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt
     8341 tcgtagccgg attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg
     8401 gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat
     8461 gcgctgatac cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg
     8521 aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc
     8581 atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaatcaga
     8641 atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga
     8701 actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag
     8761 aaggccatca gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa
     8821 tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga
     8881 gcgaagttga taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg
     8941 ccggagagga cgtagaccgt ttccataatg gccaggagga acgccatacc gagcgtcagt
     9001 ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact
     9061 atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag
     9121 aaagggtcac acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga
     9181 atgcacacaa aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat
     9241 cccacgtata tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt
     9301 tgcatttttc gacggtgatc aatttatagc taaattgccg cctttcagcc aatttgatcg
     9361 agaacaattt atctcttttt gatgcccatt tccaagactt atacattgat aaatatcaat
     9421 ttctacaaat tatgttaatc atgtttcaac aaggtaactt tagtgacatt tatgtttaaa
     9481 atgtgtgaat tataagactg aaattagttt cggttggcaa ttaaagataa aaagagacga
     9541 agcaaaaaca gcacggttga ccttaattct ttttttggtc actggctaat acgacgcaga
     9601 gtttcaggat tattaataaa ggcataaatc tcctccccgc cgatattaac atttaattaa
     9661 cttataatta tattataatt aactcacccg gggttattat tttgttaaaa ataaagccac
     9721 atatacgtag cttgagagtg aagataaacg caaaattacc tgatgcgcta cgcttgccag
     9781 tgtctacatc atctctgcaa tatattgaat ttgcctgctt ttgtagaccg aataaagcgt
     9841 tcatgccgta tccggcatga acaaagcgcc ctttgtcaac aatctggccg cgcatatgtg
     9901 gcttcggttt gattgctatt caggcaagcc ggtaactgaa cgtccgtgac tggccgggta
     9961 aaaaaggccc cgatagattt gaaccttgat tttcctcggt ggtaatgtgt tcatccatca
    10021 tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac agtcgcatca caggttgctg
    10081 attcagccgg attaaacagc cgtaaaatca cttcttgtcg gtcttcagct ttcttaagtg
    10141 cgcttatcag gcatcccact gggggcattt tcaacaaact ataactttcc ggcacgttga
    10201 atccggcttt gttgagcttc atcgcatccc atgggatttt gttgtaacac tgtactggag
    10261 tcagccatgc tcgcgcctgc tgagctacac cagcggcggt tggcgtaccg gtataactca
    10321 ataaacttag gcgacaagaa agcagaccac gtagttgtga gtccgggact ggcattttaa
    10381 ttcccgaagg ccgcctaggc cttaaaagca gatcttcttt ccccagtaag cccacgccgc
    10441 gcagcaacgt aatggcaaag gttttcttct cttcaccgat gacttcaaat tcacgtagcc
    10501 cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa ggctgcatag ttgagcatat
    10561 tccataccgg aaccggcgct tctttccagc cttcttgctg ccagttgttc attgcactgt
    10621 cgttcacggg gcgcgttagc gaaccaaact gggtatctgc cagaacacta tcggtgttaa
    10681 aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc ctggttatca aggttgatat
    10741 ccacatcaat gcgcctgttg ttatgactaa gagtgacaac caactcgaca cctactctgc
    10801 cagtggattg tcttgcgctg cgttgttgca gattacgcgg caccgccatt tcatagcgga
    10861 taacagccct gctctgccag gcctcatgaa taatctcgca ttgcggtttc gcattcgccg
    10921 aagtaattac ccactcttct tttgcgggtg aatagtcata ttcatcacca tcatcagagc
    10981 tttcttcaat ttgcaacacc cggtcatagc gcacaccgct atctttatct accagttgta
    11041 gcgttccatc ctcattgagc gcaatttgcc agaaagcatt ttccagtatc ccttcagcgt
    11101 cactttttgc cgcaatcacg ttgccaggct gattcgcttc gatataaagc gtgcgatagc
    11161 ccatagaagg gacaatctgg ttgatctgta tatcaaactc cataaaggga tcgtaattac
    11221 cgtaatgaac tatttgccga tcgattaggc ctggatcgat ctcacgggca tggcgaataa
    11281 aatacggtac aggctgaccg cgatcgtctc gtagattaaa ctggctggcg cgcagccgca
    11341 cagtggtgtt gatcacttct tcccgcggcc agggcatcag gttaaacagg acgagtttgt
    11401 cggcgtcgct ctgcggcatg ttgtcggcga ttttacgcat atagaaacgc agcagattat
    11461 ccgccatgtc ttcagccagt tcgaagcggg cgacgatttc gcgatgaact ttgtcactac
    11521 agcagcagcc gatgctgtcg tgggcatgat tttttaagat ctctttccat attttctcca
    11581 gcaagccgtg gtggtattca aaacccaacg tccaggccag cgttgccagc ggttccagcg
    11641 gttccagcgg ttccagcggt tccagcggtt ccagcagatt aacaatctta ttttcaatac
    11701 gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc acgcgcatat
    11761 atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc gcttcgatct
    11821 tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa atttcgcgca
    11881 gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc ccattcggca
    11941 gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg tcgaggcgtt
    12001 tgcggaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc aacggcatca
    12061 cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt ttatccgtac
    12121 cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat ccattgtaga
    12181 tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt ttcatcggct
    12241 cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg gattccgcag
    12301 aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg ccggcttcta
    12361 ccagtttctt cacgcggtct ttgttttccg gtttcaccgc gaaataatct tcgaggatcg
    12421 ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt cggcacagga
    12481 tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa taccactccc
    12541 gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc gtcgtttcct
    12601 ttttgtttat tgcattacgc catcagtcag atagttgcca tgcttaaccg cgtgacgccg
    12661 ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac caaaccagcc
    12721 aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg agaaaatgcc
    12781 agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg ttaccataga
    12841 gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg ggatcgcccc
    12901 ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa tttcaaactc
    12961 tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg aaaccatttt
    13021 gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc ccaggcagaa
    13081 tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa aaccgagaat
    13141 ggcacccagc aacagcgcgt tacttcctga cagagcgttc agccaggcgg taagcgagtt
    13201 attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg ctcccaatgt
    13261 accgagcacc aggtagagat aaaaagtcag gaagccattg aatttactgc taagacgcaa
    13321 gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga ccgcgccgag
    13381 aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg ctaacgccgg
    13441 tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca aaattccgag
    13501 cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat taaacagatc
    13561 ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc cccccgcgac
    13621 aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct ccgttttgac
    13681 gcttttcacc ggttgcgtat cttgctgtac ggtacgcgtc tcatcgccac gctcgagggt
    13741 aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag gcacagaaag
    13801 cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg caaaaataca
    13861 ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt tagcaccttg
    13921 cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat actccgcagc
    13981 catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag ttggcgcgtt
    14041 ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga gcaattcatc
    14101 aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc gcgtggtcag
    14161 cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg gggggaatcg ccagcaacac
    14221 cactaaatca accgcttccg ggccatccac gctttcccac tgaagcggct cgctgagtgt
    14281 ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca cagccaaccc
    14341 ttcacctaag gcggtcgggc caaggctttc acggcgatac acttctttca gaaattgctc
    14401 agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa tggcctcttc
    14461 acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag tcgtcaggtt
    14521 catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgat aaaacaatac
    14581 cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tgtatttgta ttatttacag
    14641 gatgaattta tcgggcataa tggctaaatt cgtattaatg agatgctaaa taatgggaca
    14701 caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc gtggcgagct
    14761 taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg gcgtcagccg
    14821 ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg aaagcattca
    14881 gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc agttaaccag
    14941 ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc tgatatttga
    15001 agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc aggatcgtgt
    15061 gtggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg aagaaacctg
    15121 gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt cgaaatatca
    15181 ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa tcattcctct
    15241 gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc cgattcttga
    15301 aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc gcaacgcttt
    15361 taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc gataagcaca
    15421 aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg ccattcctgt
    15481 agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac agttttcagt
    15541 gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc cagagcagcg
    15601 aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg gcccatacgt
    15661 ttgcctttcg gcgcagtcac accagcgatg taacccacaa ctggcttggt aacatgctct
    15721 ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat catcacgatc
    15781 gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt agagccaggg
    15841 atcgggtcac cgccgatacc gacacaggtc gactgaccga aaccgtaatc cgtggtctgt
    15901 ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt acccggtttg
    15961 tgaatgtgac ccggctggat accgattttg cattcacccg gggtgataac gcctgggcag
    16021 ttcgggccga tcatacgaac gcctgcttcg tccagtttca ctttcacggt cagcatatcc
    16081 agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc gatagcttcc
    16141 agaatggagt ctttgcagaa cggtgctggt acgtaaataa cagacgcgga agcgccagta
    16201 gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt agtgccgcct
    16261 ttacctggag ttacgccacc aaccatttta gtgccgtatg caatggcctg ttctgagtgg
    16321 aaagtcccct ggctaccggt aaagccctgg cagataacct tggtgttttt atcaattaaa
    16381 atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc gtcagacctt
    16441 ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt tcggcgttgt
    16501 taccttccag acgtaccacg accggtacgt taacacccac ttccgctacc gcgccgatga
    16561 taccgtcagc gatcaggtcg caacgaacta taccgccgaa gatgttaacc agaacggctt
    16621 tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct ttggttgcgc
    16681 cgccgccaac gtccaggaag ttagccggtt cgccgccgtg cagtttaact atgtccatcg
    16741 tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc agcgcaacgt
    16801 agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac tggtcacgca
    16861 tttcgcgcag atcaggctgg cggaacaggg cgttgccatc agcgcccagt ttgccgtcga
    16921 ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc agcgccaggt
    16981 cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc tgaaccagtt
    17041 taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc gggccagtca
    17101 gcggatcaag cgcaacttta tggatcaggt gcggagtttc ttccgccact ttttcgattt
    17161 ccacgccgcc ttcggtggag gccataaaga ccacgcgacg ggaactacgg tctacaacgg
    17221 caccgagata cagctcttta gcgatatcgg tcgccgcttc aaccagaatc tggttaaccg
    17281 gttggccatt ggcatctgtt tgatacgtta ccagacgctt gcccagccag ttttctgcaa
    17341 aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta ccgcggccac
    17401 cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa gcggcttctt
    17461 ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca tagcgggcaa
    17521 acagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct tcagtaatcg
    17581 ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc aggtgaagct
    17641 taaactacta cacgtccagc agcagacgcg tcggctcttc cagcaactct ttgatcgtta
    17701 ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac agcgccaggt
    17761 acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct ttgatagcgt
    17821 gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc agggaaccga
    17881 acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc gtcagcttgc
    17941 catcacggcc tttgactgcc agctctttga ttttcttctc gatgtctgcc atgccgaggg
    18001 tatcgacatc acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc gccatgctga
    18061 cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat agacgcgttc acttccgggt
    18121 aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc agacggatgc
    18181 cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc ggcttcatgt
    18241 tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc agacgctctg
    18301 ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca gccagagccg
    18361 gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct ttcgccagat
    18421 gtttttccac atcttcacga gtcagacgac cacccacacc ggtgccttta atggcgctgg
    18481 cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca tcgttgtttt
    18541 gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct tcagatttgg
    18601 cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc tgacgagacg
    18661 ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct gatgccggta
    18721 cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt acgactgcgt
    18781 cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca ggcaggtcag
    18841 ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt tcagcgcgtc
    18901 attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg ccggagaggc
    18961 ggaggccggg cggcctgcat aacgcagaga agccccaaac ggaatcactt cacggaaatg
    19021 atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc agacaaaatc
    19081 cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga acgggtagag
    19141 ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct gttccagcag
    19201 gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg gatcaagttc
    19261 gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga ggctggaaac
    19321 cgccagcgga tgacgcagca gggatttcgg cgacatcacg accagcggac gacgcatccc
    19381 gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg gtacgcaaac
    19441 ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg aggagtgctc
    19501 cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca tccggcccca
    19561 tttctgctcg ccagaggaga tgaactggtc gataaccacc tgcgcaccgt tggcgaagtc
    19621 accgaactgc gcttcccaga tggtcagggt gcgtggttct gcggtggcat aaccatattc
    19681 aaacgccagc acggcttctt ctgacagtac ggagtcccag acacggaacg cgccctgacc
    19741 gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt ggatcaccgc
    19801 gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa ccggaatgcc
    19861 ttcatcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga acagtttctc
    19921 accggctgcc atcgcctggc gatcgccata aatcttggca acgcgagact gcatttcaac
    19981 tgcttctggt accgtactga tacgtttagc cagttcctgc aggcgcttca tctcaacttt
    20041 gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga aagagtgcat
    20101 gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat cgcggtacag
    20161 gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca gcttgtcagc
    20221 gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagtg gctgggttgc
    20281 gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cataccaggt cgatgaagac
    20341 atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg caacggcttc
    20401 cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga tatcagtaca
    20461 gtacggtgta gaacgggcat ccagcgggtt agaggtggtg aaaccaacct ggttgttgat
    20521 aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt tcagagtttc
    20581 ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg gcagcacttt
    20641 gttgctgctc ggctcatcaa gtctgtccag acgggcacga acagagccga taacgaccgg
    20701 gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca ggccgccatc
    20761 ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc cgaggtgttc
    20821 tttatgttta ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca gcacgttcac
    20881 cagcacgttc agacgaccac ggtgcgccat cccgagaacc acttcgcggg tgccgctgtt
    20941 gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc cttccagcga
    21001 gaagcgtttt gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt cagcggcggt
    21061 cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac cagactcgat
    21121 acgctgttgg atccagcgtt tttcttcggt gctggtgatg tgcatatact cggcaccaat
    21181 cgggccgcag taggtttgct tgagggcgga aataagctcg ccgagtttca tggtttcttt
    21241 gccgctggca aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg tcagatcgtg
    21301 gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg gatctagatt
    21361 cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt taacctgctt
    21421 cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt tcgccaggcg
    21481 gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg taccaggtaa
    21541 ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta agaagtcttc
    21601 atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt ccaaccaggc
    21661 tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct tacttcgccg
    21721 tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct cttgtctacg
    21781 tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt ttttaaagat
    21841 ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctgcg ggtaatgact
    21901 tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg caacgcttgc
    21961 gcgtcttatc aggcctacag tttacgcatt acgttgcaac aacatcgact tgatatggcc
    22021 gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca tgatgctgtg
    22081 acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag tctcggtatc
    22141 acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga taaacttatc
    22201 gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa tacattcata
    22261 cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag ctggcggatt
    22321 ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga attgtcccat
    22381 gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca caatcttctt
    22441 gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct tgccgttcat
    22501 gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg acaggctggg
    22561 atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac cttcttccgc
    22621 ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat agcgataaat
    22681 tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct tcggcgggaa
    22741 tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt ccgactcagg
    22801 cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt cgaagcggct
    22861 atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat acgccgtttc
    22921 catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc tggaagtgtc
    22981 atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa gccctttcgc
    23041 catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt gcagcgcttt
    23101 acggatcgcc accggatctt caccgttacg attattgttc caccggttca ggcgatccag
    23161 agacgcttca acatcagact cgctggcatc gcgcagtgcg ccctgctcgg cgatagactc
    23221 ttgcagatgc agacctgccg cgcgaccaaa gaccaccagg tccagcagcg agttgccgcc
    23281 cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa acagtcccgg
    23341 aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa ctttggtcgg
    23401 aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt tcaccggatc
    23461 gacgtgagcg aaggtacggg aaagctccag gatacccggc agacgggatt cgagaacttc
    23521 tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat cacagccgcg
    23581 accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg ccaggtcttt
    23641 ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca gataaccgcc
    23701 ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg tcgggtggaa
    23761 ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc cgacaccgtc
    23821 gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgccc cgccagtcgc
    23881 cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc acagtgcggt
    23941 acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc actcggagaa
    24001 aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg cgtgaccggt
    24061 acggtcagca gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt tcgactgacc
    24121 gccaaacgga cgttgataga tacgaccatc atcgagacgc gagaacggca ggcccatgtg
    24181 ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg cgtcctggtc
    24241 accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt tatcttcatg
    24301 ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg aacgggtcgg
    24361 gaagacttta gagagcagcg cacaggtctg gccgctctgg gaaatttgca gcgccgcgcg
    24421 catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg gcaatttcat
    24481 cacacccccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc aacgacaatc
    24541 accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa cacctgccac
    24601 atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa cactttggtg
    24661 aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact ggtggcgaaa
    24721 aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg aacgaggatg
    24781 aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg aggactcctg
    24841 cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt ttacccgctt
    24901 cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct acgacgacgt
    24961 gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag ctgcccataa
    25021 tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga agccacagca
    25081 ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg gacgctatcg
    25141 ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt tttttcacat
    25201 ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac tacgggcaca
    25261 gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc tgcttgcggg
    25321 tcagacagcg tcctttccat aactgcgcgt catgcaaaac actgcttcca gatgcgaaaa
    25381 cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac ctggcggtag
    25441 tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg gttttatcct
    25501 gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc taatttgaca
    25561 atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg taaaagttat
    25621 gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt gaccaattca
    25681 ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag tacttgattc
    25741 gccatttatt cgtcatcaat ggattcttta cctgcaagcg cccagagctc tgtacccagg
    25801 ttttcccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc aggttgatgt
    25861 gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga gaccttaaat
    25921 ggctgataca aaagcaaaac tcaccctcaa cggggacaca gctgttgaac tggatgtgct
    25981 gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa aaggtgtgtt
    26041 cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta cttttattga
    26101 tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg cgaccgattc
    26161 taactaccta gaagtttgtt acatcctgct gaatggtgaa aaaccgactc aggaacagta
    26221 tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga ttacccgtct
    26281 gttccacgct ttccgtcgcg actcacatcc aatggcagtc atgtgtggta ttaccggcgc
    26341 gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc gtgaaattgc
    26401 cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca agtattccat
    26461 tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact tcctgaatat
    26521 gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac gtgctatgga
    26581 ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca ccgtgcgtac
    26641 cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg cttcactgtg
    26701 gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag aaattagctc
    26761 cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt ctttccgcct
    26821 gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg taatgcgtga
    26881 aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg aagtggctat
    26941 ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac tgtacccgaa
    27001 cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt ccatgttcac
    27061 cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg aaatgcacag
    27121 ttacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa aacgcgactt
    27181 taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt aacccgccgt
    27241 tcaaatggcg ggttaatttt tatatgccta aacacaaaaa attgtaaaaa taaaatccac
    27301 tatcggacat tcatatattc taaaaaaaaa tatatcggta taaattataa acaaatcgat
    27361 gatttttaat catctatctt cctctcagtt acatgaataa accaccaaat atagcgcacc
    27421 cctataattt ccaatacttt attcatcaaa catttaattc ataccatcaa ttaattactt
    27481 ttacatttaa taaaattaat aatattttta agaaaaacta aaaacattcg acaatcaaat
    27541 aagatattct tgcaccgata tacttttttc ttttctgtta aaggactcag ttatgtttaa
    27601 aggacaaaaa acattggccg cactggccgt atctctgctg ttcacagcac ctgtttatgc
    27661 ggctgatgaa ggatccggtg aaattcactt taaaggtgaa gttattgaag caccgtgtga
    27721 aatacatcag gatgatattg ataaagaggt tgaactcggt caggtgacca ccagccacat
    27781 taatcagtca catcacagcg atgccgttgc tgtcgacctg ctcttagtca actgtgatct
    27841 ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa gttgcagtca cctttgatag
    27901 ctcagcgaaa accaccggcg cagatccgat tctcaacaac accagcacgg gtgaagccac
    27961 cggcgtcggc gtacgtttaa tgaataaaga tcaaagcaat atcgttttag gcaccgctac
    28021 tccagatatc gacctggctc cgacctccag cgaacaaacg ctgaatttct ttgcctggat
    28081 ggaacaaatt gatcaggcta cacctgtaac gccaggcgca gttacggcga atgcaaccta
    28141 cgtgctggat tataaataag tattactttg gcggcgctat cgccgccttt cataaaatat
    28201 ttatcaggta tggacaccgt gaatatttat cgactctctt ttgtatcctg cctggtcgtg
    28261 gcgatgcctt gcgcattggc ggtcgaattc aatctgaatg ttctcgataa atcaatgcgc
    28321 gaccgcattg atatttcatt attaaaggaa aaaggagtca ttgctcccgg tgagtatttt
    28381 gttagcgttg cggtaaataa taaccaaatc agtaacgagc aaaagattaa ctggcacaaa
    28441 aatgacgata aaaccattcc gtgcatcaat gatttactgg tcgataaatt tggcttaaaa
    28501 cctgaagtcc gtcagtcatt accattgata aatcagtgcg tcgattttag ctcccgacct
    28561 gaaatgctct tcaatttcga tcaagccaat cagcaactga atattagtat tccgcaagcc
    28621 tggctggcgt ggtactcaga aaactggacc ccaccctcca catggaaaga aggtgtcgcc
    28681 ggtgtcctga tggattacaa cttgtttgcc agcagctacc gcccacagga tggcagcagc
    28741 agcactaatc ttaacgccta tggaacagcc ggaatcaaca cgggggcatg gcgcttacgc
    28801 agtgattatc agttgaatca tactgatagc gatgataacc atgaacagtc aggcgaaata
    28861 tcacgtacct atctttttcg cccgttaccg caattaggtt caaaattaac cctcggcgaa
    28921 acggatttta gttccaatat tttcgacggt ttttcttata ccggcgcggc actggcaagt
    28981 gacgatcgaa tgttgccatg ggaactacgc ggctacgccc cacaaattag cggtattgca
    29041 cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg tcatttacca gaaaaaagtc
    29101 ccaccaggcc catttatcat tgacgacctt aatcagtctg ttcagggcac actggatgtc
    29161 aaagtgacgg aagaagatgg tcgggtgaac aatttccagg tttcagcagc atcgacgccc
    29221 ttcctgactc gtcagggaca ggttcgctat aaactggccg cgggtcagcc acggccctcc
    29281 atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg ggggatgctg
    29341 tcaaacacct cgctgtacag cggcctgctg ctttctggtg atgactacca ttctgccgca
    29401 atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt cgtttgatgt cacgtgggcc
    29461 agtagccatt ttgatactca gcaggacgag cggggcttaa gctaccgttt taattacagc
    29521 aaacaagtgg atgccactaa cagcacgatt tccctcgccg cttatcgttt ctccgatcgt
    29581 cattttcaca gctacgccaa ctatctggat cacaaataca acgacagcga tgcgcaggac
    29641 gaaaaacaga cgatcagctt atctgtgggc caaccgatta ccccactaaa cctcaatctt
    29701 tacgccaacc tgctacatca aacctggtgg aatgcagacg cctccacgac cgccaacatc
    29761 acggcaggtt ttaatgttga tattggtgac tggagagata tctctatttc tacgtcattc
    29821 aatacaaccc attacgaaga taaagatcgc gacaaccaga tttacctgtc gatttcgctc
    29881 cccttcggta acggtggtcg ggttggctat gacatgcaaa acagtagcca cagcaccaca
    29941 caccgcatgt cgtggaacga tacgctggat gaacgtaata gctggggcat gtctgccgga
    30001 ctgcaatccg accgtcctga caatggagcc caggtgagcg gtaactatca gcacctgagt
    30061 tcagcgggtg agtgggatat ttctggtacc tatgccgcca atgattacag ttccgtcagc
    30121 agcagctgga gcggttcttt caccgcaacc caatatggtg cagcgtttca tcgccgcagc
    30181 tccaccaatg aaccacgcct gatggtcagc accgatggcg tggcagatat tccggttcag
    30241 ggcaatctcg actacaccaa ccattttggc attgcggtgg tgccgttgat ttccagttac
    30301 cagccttcca ccgtggcggt gaacatgaat gacttacccg acggcgtaac agttgcagaa
    30361 aacgttatca aagaaacgtg gattgaaggc gcgataggtt acaaatcact ggcttcccgt
    30421 tccggtaaag acgttaacgt catcattcgc aacgccagcg gtcagtttcc tcccctcgga
    30481 gcggatatcc gccaggatga cagcggcatt agcgtgggga tggttggcga ggaaggacat
    30541 gcctggttaa gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg tgatagccag
    30601 tattgctcgc tccatcttcc tgaacatatg gaagacaccg caaatcgcct gattttacct
    30661 tgtcattaat taaggaaatg aaaatgacat ttatgaaagg actacccctt ttgttgttgg
    30721 ttgccagtct gtgcagccac gctgcactac aacccgatcg cactcgtatt gtgtttaacg
    30781 ctaatgataa agctaccagt ctgcgggtgg ataatcgtag cgataaactc ccctacctcg
    30841 cttattcctg gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc
    30901 cgccgataca acgtctggaa ccgaaagcaa caacgcaggt acggatagta aagcaagcct
    30961 cgaccacaaa attaccgggc gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc
    31021 ctcccgcccc ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt cagagtcgta
    31081 tcaaggtgtt ctggcgaccg gctgcattac gcaagaaagc aggagaaaag gtcgaactgc
    31141 agttacaggt cagccagcag ggcaaccaac tgacactgaa aaatcctacc gcgtattatc
    31201 tgaccattgc ctacctggga cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga
    31261 tggttgcgcc ttttagtacg gtcaacacaa acaccggaaa ttatagcggc agccagtttt
    31321 atctcggtta tatggatgat tacggcgcgt tacgcatgac gacgctcaac tgcagcggac
    31381 aatgccattt acaagccgtg gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt
    31441 catctgtctg ttatttctgc cgttgaagtc tgcgcttgcc ctgaactgct actttggttc
    31501 atccggtggc tcggtagaaa aatcagaagc gattcaacct tttgctgtac caggcaatgc
    31561 caaactcggc gataagatct gggaatctga cgatattaaa attcccgtct attgtgacaa
    31621 caataccaac ggcaactttg aaagcgaaca tgtttacgca tgggtaaacc cctacccggg
    31681 ggtgcaggat cgttattatc aactcggagt aacttacaat ggcgttgatt atgacgccaa
    31741 ccagggaaaa agccgcatcg acactaacca gtgtatcgac agtaagaaca ttgacattta
    31801 tacccctgag cagatcatcg cgatgggatg gcagaacaaa atttgctcgg gtgatcccgc
    31861 taatattcac atgtcgcgta cctttctcgc tcgcatgcgg ttatacgtca aaatacgaga
    31921 aatgccgccg catgattatc aaagtacgct tagcgactat atcgtcgtgc aatttgacgg
    31981 tgccggtagc gttaacgaag accctactgc ccaaaacctg aaatatcata ttactggtct
    32041 ggaaaacatt cacgtgctgg attgcagcgt caatttttcc atttcaccgg aaacacaggt
    32101 gattgatttt ggtaaattta atttgctgga tattcgtcgc cacacaatgt cgaaaacatt
    32161 cagtattaag acgacaaaat cacaaaatga ccaatgcacc gacggattta aagtcagttc
    32221 ctccttttat accgaagaaa cgttggttga agaagataaa gcattgttga ttggtaatgg
    32281 tctgaagcta cgtttgctgg atgaaaacgc ctcgccttat accttcaata aatatgctga
    32341 atacgccgat ttcaccagcg acatgttggt ctacgaaaaa acctatacag ctgaactttc
    32401 gtccattgca ggtaccccca tcgaggctgg gcccttcgat acagtggtgc tttttaaaat
    32461 taactataac tgatttgcca tgatgccctt tgcagccgga aagggcatct ctttcagaaa
    32521 attgctgatt ttcccctttt ccttgattgc tgttattttt tcctagcccg gctttcttta
    32581 tgctgtatcg atacaccaaa gagaaaataa tgagggagcg aaggatgcca gttttgcagt
    32641 ggggaatgtt atgtgtgcta tcacttctcc tttctattgg tttcctcgcg gtacatctcc
    32701 cggcggcgct attacttggg ccgatgatcg ccgggatcat cttcagtatg cgcggaatta
    32761 ctctgcaact tccccgctcc gcttttctcg ccgcgcaggc cattcttggc tgcatgattg
    32821 cgcaaaacct cactggttcg attctcacca cgctggccgc caactggccg atcgtgcttg
    32881 ctattttact ggtgacgctg ctttccagcg ccatcgtggg ttggttattg gtgcgctata
    32941 gctcactgcc tggaaatacc ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg
    33001 tcgctatggc gcaagattac ggcgcagata ttcgcctggt ggcgtttatg caatatctgc
    33061 gagtgctgtt cgtcgcgggg gccgctgttc tggtcacccg catgatgctg ggtgataacg
    33121 ctgaagcggt taaccagcaa atcgtctggt tcccgccagt gagcattaat ctcctgctta
    33181 cgattttgct cgcagtcgtt gccggtacgg cgggatgcat gctgcgtctt ccttccggca
    33241 cgatgctcat cccgatgctg gcgggcgcag tgctccagtc tggtcagctc atcaccatcg
    33301 aactaccgga atggctgctg gcgatggcgt atatggcaat tggctggcgg attggtcttg
    33361 gtttcgataa acaaatctta ctgcgggcac tacgcccgct accgcaaatc ctgctgtcga
    33421 tttttgctct gctggctatt tgtgcaggta tggcgtgggg actgacccgg tttatgcata
    33481 ttgattttat gactgcctac ctcgccacca gtcccggcgg gcttgatacc gtggcggtca
    33541 tcgccgcagg gagcaatgcc gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt
    33601 tcagtatttt gctgacaggg ccagccattg cgcggtttat ttcaacctat gcgccgaagc
    33661 ggtcggccta gtgctggcag cctggacacc agtaaaacgg gcgcgaggac aacgtggttt
    33721 tctcaatgat gccgccacag cgttcgcaca gttcgccatc acgatgaaaa accttaaagc
    33781 gaaacagcgc cccatgatgc ttattctcat ccacctgccc ccgcgtagcg taagaaaacc
    33841 gaggaatctc cagtaacgcg tgtgcgagtg catccagttg cgccgcattg agatctttcg
    33901 ctttatgatt tccagtcaac ccaacctgcc agaggatctc cacccgcaaa taattgccaa
    33961 gcccagccag aaacgcctga tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg
    34021 gcgacaataa ccgttccttt accacctccg gcgtcagatt cggatccagc acatcgggac
    34081 caacgcgttg taaaaacgga tgcgtggtca gttgttccgg cctcaacatc tcaatatccg
    34141 aggcgctata aagcagaatg gttttgtcag ccgtttgcag ttttacccgc aatactcgcg
    34201 tggtctgcgg ctcttcgccg gtatcaacca cgcgccagac gccgtaaagc tgattatggc
    34261 tgtagagcgt taaaccgttg ggaaaatgag ttaacaacgc cttaccacgc gtttccacat
    34321 gggtaacgtg ttgaccgata agttgtgatt gataagtttt taactgcggg aaggcaaacc
    34381 agacatcagt tagtggtttg cctttgatcg ccgcctccag gttatccgct gcacggcgga
    34441 tctccgggcc ttcaggcatg gtgatatcct tttttcattc tttattaagc ggggttatgc
    34501 tgcgacaaca atcccctttt tggcaaatgt agctcgtaat cggcgggcga acgccagagc
    34561 gtgctcgccg tcgccatgca ggcagaccgt ttgcgccgcg accgttgccc attcgccggt
    34621 gatacttttg actctgccgt gttgcaccat ctccagcgtt tgcgccaacg cctgttcttc
    34681 gttttcaatc aacgcgcctg gctgacttcg cggcaccagc gagccgtcag cctgataacc
    34741 gcgatcggca aacacttcct cgcgcgttgt caggccatat tgcttgcctg cacgaatcag
    34801 ctcgcttcct gccagcccaa cgagaaccaa tgccggatcg caagcgtata ccgctctggc
    34861 gatggcgtct gccagttgtg tttctttcgc cgcctggttg tacaacatgc cgtgcggttt
    34921 gacatgacgc attacgccgc cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata
    34981 cagcgtctgg gcgtaaacgg tttccggcgg cagctgcatg gcgctgcgac caaaattttc
    35041 cctgtcggga aaactcgggt gagcgccaat cgcgacacca ttttttattg cttcacgcac
    35101 gcaagcctgc atggtttgcg catcgcctgc atgaaatcca caagcaatat tggcagagga
    35161 aaccagcgtt aatagctctg cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc
    35221 aattttcatt gtgcagccgc cacgctaatt gttcaaaata acgttgctga tcttgccgcg
    35281 ctttcagtgc ctcttccagt gaacactgga caaaatgaat cggctgaccg agcggaattt
    35341 gcgccagatg gtacatatca gcctcaatga tacaggcaat acgtggataa ccaccggtgg
    35401 tctgtgcgtc gttcatcaac acaatcggct gcccgttatg cggcacctgc accacgcccg
    35461 gtaacaaacc gtgagataac agttcgcgat cggtgatgcg ttttaaaatt tgcccctgta
    35521 aacgatagcc cattcggtta ctttgcgagc taagctgcca gggcgaacgc cagaatgcat
    35581 cctgcgaggc gcgatcgaac tcatgatatt ccggccccgg caaggcgcga atgcggttgc
    35641 cccacagcag ctgtttaacg ccctgcgctt ccataaaatc acgcttcgat ttgccaatcg
    35701 ggagtcggtc accatccttc agtaaacggc cttccagccc gccaatcccc actttgagat
    35761 cggtgctgca tgaccccatt accggcggaa catcaatacc acccgcgacc gccagataac
    35821 tgcgcatccc gtgctgcggg cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc
    35881 gccagccggt ccagacggcg ttatcatcca accgcgcttc gcaacctgca cccgtcaggg
    35941 caaaccaccc atcagtttcg aactcaactg ttaactgacc gagcgtgatc tccagcgcgg
    36001 gggcattggc gtcattaccc accagcaggt tagcaatgcg cagcgcaggc atatccagtg
    36061 cgccacagtg gctgataccc gactggcgaa aaccgtgacg accgccatcc tgcacagtgg
    36121 tgtacatgcc cgcacgaata atcttcagca tattccctcc ttctgcggta caaatcgcac
    36181 gctgtctccc ggacgtaata agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga
    36241 ggtatgacca atcaactgcc agccacccgg cgttgccagc ggataaacac cagtctgcgg
    36301 cccgccgatc ccgacagaac ctgccggaac gagcaagcgc ggttcagcgc gccgtggcgt
    36361 gtgtagttgt tccggcaaac tcccgagata cgggaagccc ggttgaaaac ctaaaaacca
    36421 gaccacgtat tccacggagg agtgcaattc aacaacctgt ttttcgctca acccgcaata
    36481 cgccgcgacc accgccaaat ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat
    36541 aaagcgagac tcaggttcca gggcctcgct ctcctcccac cagcgttgca aacgctctat
    36601 ggcatccagc gccagcgact caggattacg caaaatcacc gtgatattgt tcatgccggg
    36661 aatggcttca accacattcg gcatatccac cagacgctgc gccagtcgcc agatccgctt
    36721 ctggctagcc agcgtcaccg gcggttccag ttccagcact accgccgttt cacctatcag
    36781 ataacaacgc gctcgttgca ctttcgatcc ctctgattat tcgttatgca ggattaggaa
    36841 tatcaataaa ggtcacatca agatcggtat tttcattcag ccactcgctc aatgcgcgaa
    36901 taccaccgcg ttcagtggca tggtgacctg cagcataaaa atgcaatccc tgctcgcggg
    36961 ctgaatgaat ggtctgttca gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg
    37021 cggcgctatc gataaaactt tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct
    37081 caggtccggt atcgccacac cataacggct tacgtcccag acgcgcttca atccaggagg
    37141 ctagttccat tcccggcaca ggcatggtca gctctcccca cggcaccaac ggctcaattt
    37201 cgcccatgac cgtgatcccc agtaacgccg ccagctgtgc gttattgccc agctcaggat
    37261 gtgcgtcaag cggcaaatgc cagccataca ggttgatatc attctccagc aacgttttta
    37321 aacggttacg cttcattccg cgaatgaccg gagactctcc tttccagaag tagccatgat
    37381 gcacaatgac cgcatccgcg cccagacgca ctgcctcatc gagcaaagcc tggctggcgg
    37441 tgacaccggt aacaattttt tgcaccgtct ctttgccttc cacctgcaaa ccattcggcg
    37501 cgtaatcact aatcgccgcg ctgttcagtt tttcgttaat cagttgttcc agttcggtgt
    37561 ttttcatctt cgcgccttcc gtattctgat agctacaccg ttaacatagc gttacctcgc
    37621 ctgttcgtcg atagccaatc ttttgacata ttaagaaaca aacccgacgc caaaagacgc
    37681 aaattgttta tatcatcacg cttcagctaa tgtatattta tgcaatcaga cagcatattg
    37741 cataaataat cagatctgta tcaaagtttt aactttaagg aaacacaagc acgccgcgca
    37801 actattacgt gacgttgccg tcaattgtgg aattattatg aataaacacg catcacaacc
    37861 gcgcgctatt tactatgtcg ttgcgctgca aatctgggaa tattttagct tttacggcat
    37921 gcgtgccctg ctgattctct atctcaccaa tcaactaaaa tacaacgata ctcacgccta
    37981 cgagttattt agcgcctact gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt
    38041 ggcggataaa gttctcggca atcgcatggc ggtgatgctg ggggcgttgt tgatggcgat
    38101 cggtcatgtg gtgctgggtg ccagtgagat ccatccgtca ttcctctatc tgtccctggc
    38161 gattatcgtc tgcggctatg gcctgtttaa atctaacgta agctgtctgc tcggcgagct
    38221 gtatgagcca accgatccgc gtcgtgatgg cggtttctcg ctgatgtatg cggcgggtaa
    38281 cgtggggtct attatcgcac ctatcgcctg tggttacgcc caggaagagt acagctgggc
    38341 gatgggcttt ggcctggcgg cggttggcat gatcgcgggt ctggtcattt tcttatgtgg
    38401 caatcgtcat ttcactcata cccgcggcgt taacaaaaaa gtactgcgtg cgacaaactt
    38461 tctcctgccg aactggggat ggctgctggt tctgctggtg gcaacgcctg cactgattac
    38521 cgtgctgttc tggaaagagt ggtcggtata cgccttaatt gtcgcgacta ttattggcct
    38581 gggtgtactg gcaaaaattt atcgcaaagc agaaaaccag aaacagcgga aggagctggg
    38641 gctgattgtg acgctcacct tattcagtat gttgttctgg gccttcgcac aacagggcgg
    38701 tagctcgatt agcctttata tcgaccgctt cgttaaccgc gatatgtttg gttataccgt
    38761 tccgaccgcg atgttccagt cgattaatgc cttcgcagtt atgctgtgcg gtgtgttcct
    38821 ggcgtgggtg gttaaagaaa gcgtcgcagg taatcgtacc gtgcgcatct ggggaaaatt
    38881 tgctcttggc cttggcctga tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg
    38941 gtccgcaatg tatggtcact cttctctgcc actgatggtg ttaggcctcg cggtgatggg
    39001 ctttgcggaa ctgtttatcg acccggttgc catgtcgcaa attacgcgca ttgaaattcc
    39061 gggcgtgacc ggcgtattaa ccggcatcta catgctgctt tctggcgcga tcgcgaacta
    39121 tctggcgggc gtgattgccg atcagacatc gcaggcttcg tttgatgctt ccggggcgat
    39181 caactactcc atcaatgcat atattgaagt atttgatcaa attacctggg gcgcactggc
    39241 gtgtgtagga ctggtactga tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc
    39301 gctggcgctg gagtcataat aggccagcac cgatgcctga tgcgacgctt gacgcgtctt
    39361 atcaggccta cgtatttcct gcaatttatt gaatttgcac aaatttgtag gctggattag
    39421 gcgttcacgc cgcatccggc atctgggctc gattgcctga cgcgtctgtt atttcccctt
    39481 ccgcgccgcc tcatacgctg ccaacgtttg tactctcgcc tctttgtgct cgactatcgg
    39541 ttgcggataa tccagcttca cacctgcttt ctgcgcccac ttccacggct catgcaccgc
    39601 tttccctggt acatcgcgca gctccggtaa ccatcgacga ataaactcgc cctcacggtc
    39661 aaatttctcg ccctgagtta taggattgaa aatacgaaaa tacggcgcag catcagtacc
    39721 cgtagaagcc gcccactgcc agccaccgtt attagctgcc aaatcaccat caatcagctg
    39781 cgacatgaaa tatcgctcgc cttcgcgcca gtcgatcaac aaatctttca ccagaaaact
    39841 ggctgtaatc atccgtagcc ggttatgcat ccagccagtg ctgttaagct gacgcatagc
    39901 ggcatcaata atcgggtatc ccgttttgcc tttctgccag gcctgtaaat gtgcgggatt
    39961 gctttgccac tgcacacgat ccgtccaggc aataaatgga cgatgtttac acaacgaggg
    40021 gtaatacgtc atcagatgac ggtagaactc gcgccagatc agctcactaa gccagacact
    40081 accggcccca ccgtccagcg cctgcggctg ttccgccaac aagcgatgca aacactggcg
    40141 aggcgataac ccgcccgttg ccaagctggc ggacaaacgg ctggtgcctt ccactgccgg
    40201 aaaatctcgt tgttgctcat actctccagc accgttctcg caaaactgcc gtaattgcgc
    40261 aatcgccgct ttttcttcca ccgggaaatg cgcagtatcg aaagactgac gaggataatt
    40321 cagcgtaatg gatggcgcgg gctttatcga tccgctacta cgaacttttg gtgcagcgac
    40381 gcactccggc atcccttccc gcagccgttt cagccaggca ttcttaaaag gcgtaaagac
    40441 tttgtacatc tcgtgattgc cggtcatcac cgcccccggc ggcaggatca cgctgtcatc
    40501 aaatccttca cacaccacgt tacgcagcgc tctttcaacg tccacatccc gcgctcgctc
    40561 attcacctca tactgatagt tataaaaaag atgagtaacg ctgttttccg cgcacacctg
    40621 tttaactatt tcgacactgg cgacaaagtc atccacttca cggaacaata aaggaatacc
    40681 tttttccgcc agcgcgattt gtagcccatt cagttgagca ttgataagtt cagcctgacg
    40741 cggcgacatg ttatgcgtct cccactggcg tggtgtagcg atatacagcg ccagcacgcg
    40801 tgcagacgaa ttgcggcagg cggcagccag tgcgagatta tcgtgcagac gtaaatcctg
    40861 gcgaaaccag gccagatggg tagtcataaa actcctgaat ggcgcaagtt aatcacacgc
    40921 ggccaaagtt cgaaataacg ctgccctgaa agccaggcgt caggataaag cgccatatag
    40981 tgtttgatac gcatcagcgg cgcaagaagt ggttgctcgc cacggcgata actgtcgata
    41041 agcgcagcca gctgctggcg ttctgtggaa tcaatatgcg ggcgaaaata accctgaacg
    41101 tgcatcaaaa cgttggtgtg atcgcgcggg ttggcgggat gagaaagcaa aacaattacg
    41161 cgttggcggt actggttgta aaaatcatcc agattttgcc actgatgaat atcagccaca
    41221 aacgggccaa gtttgcggta ctcgggctga gaatgggcca gaaagacgag tttatagcgg
    41281 ctgtgataat cgagaagcgc cccgcgcgtt aagccatgtt ctttcagtcg atgtaactca
    41341 tgaagggcga agatacggat tagggtgctg tcatcaaatc gttgtagatt cattactgct
    41401 cctgaacggt atacaggagt aagtgtagac gttggtaaat gatgtttgtg aagttaatcc
    41461 gctagcaggg caagattacg aaagcgcgct ccccgcaagc aactaatcaa ggtagttaat
    41521 catggctgaa cacgatatta cgccagaagt caccatttcc aaaacacagc gccgctataa
    41581 agtcggctat gtcagagccc gccaccagga ccgcaaaacc ggtatggccc ggtattatag
    41641 cctgcacctt aagggcgact ggctgaaaga ggccggattt gataccggac ggggtgtcac
    41701 ggtgaagatt tcggaggggg gcctgaccat catcgctgac agtgacgagg tgcaggaact
    41761 gcgggaggaa ctttatcagg ttaaacaggt agttaagggg atgcgggatg ggatgtttag
    41821 tgtgctgaac gaaagttaaa tattaaaaaa gctgggaaga tttaaaacct tcctagcttt
    41881 ttattctgtc ttattataat gtatactcat catcttccga tgaatttata aattgctcca
    41941 agtttacatg aagagtactt aacctagttt ttaactcatt aagtgaagcg ataatttcat
    42001 attttccaac ataatcatat gatggatcgt attcattatc tatgtcagaa gctaaaaatg
    42061 atgatattcg tagtttaaat ttagcttcat ctttattaca ttcattggca taaggtgaat
    42121 gccctatcat agcatattcc tctaatctaa aaggataaga tggttttaat tcatcgatca
    42181 atgaaatttc aatccatgtt ctatatccag gcattaatgg actaatttct ttgagcaaaa
    42241 cctttccact taataatgta ttga
//
LOCUS       DADLZM010000019        40514 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLZM010000019 DADLZM010000000
VERSION     DADLZM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.019"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            231..1664
                     /locus_tag="JNY73_001016"
     CDS             231..1664
                     /locus_tag="JNY73_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY8047109.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JNY73_001017"
     CDS             complement(1672..2730)
                     /locus_tag="JNY73_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8047110.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JNY73_001018"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JNY73_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JNY73_001019"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JNY73_001019"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY8047111.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNY73_001020"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNY73_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY8047112.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNY73_001021"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNY73_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY8047113.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JNY73_001022"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JNY73_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY8047114.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JNY73_001023"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JNY73_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY8047115.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLANPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRHRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="JNY73_001024"
                     /pseudo
     CDS             complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="JNY73_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="JNY73_001025"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="JNY73_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY8047116.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="JNY73_001026"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="JNY73_001026"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY8047117.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRNILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="JNY73_001027"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="JNY73_001027"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY8047118.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="JNY73_001028"
     CDS             complement(17698..18321)
                     /locus_tag="JNY73_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY8047119.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /locus_tag="JNY73_001029"
     CDS             18618..18893
                     /locus_tag="JNY73_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047120.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="JNY73_001030"
     CDS             complement(18970..19212)
                     /locus_tag="JNY73_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY8047121.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="JNY73_001031"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="JNY73_001031"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY8047122.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="JNY73_001032"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="JNY73_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY8047123.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="JNY73_001033"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="JNY73_001033"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY8047124.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22591..24288
                     /locus_tag="JNY73_001034"
     CDS             22591..24288
                     /locus_tag="JNY73_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY8047125.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24303..25037
                     /locus_tag="JNY73_001035"
     CDS             24303..25037
                     /locus_tag="JNY73_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8047126.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25050..25907
                     /locus_tag="JNY73_001036"
     CDS             25050..25907
                     /locus_tag="JNY73_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8047127.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="JNY73_001037"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="JNY73_001037"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8047128.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="JNY73_001038"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="JNY73_001038"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY8047129.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="JNY73_001039"
                     /pseudo
     CDS             complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="JNY73_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="JNY73_001040"
     CDS             complement(30571..31818)
                     /locus_tag="JNY73_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY8047130.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGENGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="JNY73_001041"
     CDS             complement(31840..32166)
                     /locus_tag="JNY73_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY8047131.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="JNY73_001042"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="JNY73_001042"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY8047132.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="JNY73_001043"
     CDS             33554..34810
                     /locus_tag="JNY73_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY8047133.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /locus_tag="JNY73_001044"
     CDS             34925..35251
                     /locus_tag="JNY73_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY8047134.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35298..36536)
                     /gene="mntH"
                     /locus_tag="JNY73_001045"
     CDS             complement(35298..36536)
                     /gene="mntH"
                     /locus_tag="JNY73_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY8047135.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36872..38074
                     /gene="nupC"
                     /locus_tag="JNY73_001046"
     CDS             36872..38074
                     /gene="nupC"
                     /locus_tag="JNY73_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY8047136.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38124..40313)
                     /locus_tag="JNY73_001047"
                     /pseudo
     CDS             complement(38124..40313)
                     /locus_tag="JNY73_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10780 a        10077 c         9382 g        10275 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaagctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggcaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcatcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgattctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc taggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgctgca gggtcgataa ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggtagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccctca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatattgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata atgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagatac cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc cgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gctacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgcc
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgtgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgaga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcttatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggtgtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccaaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagc
    22441 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    23041 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    23101 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    23161 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    23221 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    23281 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    23341 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    23401 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    23461 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    23521 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    23581 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    23641 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    23701 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    23761 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    23821 gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag
    23881 caggcccgct ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc
    23941 tgcattccca gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag
    24001 ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc
    24061 attgcggtgc gagataccgg gcacggcatc gatccaaagg tgattgagcg ggtcgaagcg
    24121 aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg
    24181 tatggcgagg ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt
    24241 cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata
    24301 ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa
    24361 ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga
    24421 agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg
    24481 atggcgtgtt gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca
    24541 tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc
    24601 tcaaaccgta tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc
    24661 aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taaaatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg caccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccatt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgta tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaagattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acctgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca gatattcagc
    35341 gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct gttcaccaga
    35401 tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc cagggcgata
    35461 ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa tcccatcaga
    35521 atgacaataa atgacggcaa catggtgact gtacgacgca cccacagcgg gatatggaag
    35581 cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt tgaggacagt
    35641 ccggcagcaa ccagacttaa cccaaagacc gttgccgcag catggcttaa cagcggttgc
    35701 agcgtcagat aagcctcatc aagatcggca acaccagtat gaccagaaaa gtggaacgcc
    35761 gccgcagctg tagccatcat cgccagattg acaaaaccgg caatcgtcat ggcgatagcc
    35821 acatcccatt tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg
    35881 agcgaagagt gcaaataaat cacatgcggc ataatcgtcg cccctaacac gcctgctgcc
    35941 aggaagaccg cctccgaagt aggtaaactc gggatcacca ttcctttacc cagctgcgcc
    36001 aggttaggct gggagaaaat caactcgaca atgtaagccg cggcaacaaa caacagtaac
    36061 ccgccaatca ctttctccag cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa
    36121 gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa
    36181 ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaataat ttctgcctga
    36241 acccaataga accacactac gggacgcgga tagtgatcac gaatctgctc cgccagattt
    36301 ttaccggtgg caatccctag tttggcagag aggatctgaa ttagcatcgc catcaggttg
    36361 gcccaaacga caacccacag tagctgatag ccgaagctag cacccgcctg aatattggtc
    36421 gcaaagttac cgggatcgat ataaccaatc gccgcaatga acgcaggtcc cattaatgcg
    36481 agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg
    36541 tgcctctaaa acatagcctt tgctatgttt catgctatgc taaacgagaa tgattatcaa
    36601 attcatttaa atggattgtg gtgatttctc tgatagacca agattatgat tacgaaaaga
    36661 ttgatgactt gaatgttatg gttatgttta atgttagcac atttacataa ctttcagctt
    36721 ccatacacaa catagcagaa atgtatgaca gatcactatt tttgaagcct gtcacaggac
    36781 gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga atgtgcacgg
    36841 aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt ttgtactggc
    36901 acttgccgtt gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat
    36961 ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct tcctgaactc
    37021 cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac tactcggatt
    37081 tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc tggcattctt
    37141 cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca ctgatcggta ttctccagca
    37201 cattcgcgtg ttgccggtga ttatccgcgc aattggtttc ctgctctcca aagtcaacgg
    37261 tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc agtcagaaaa
    37321 ctttattgcc tataaagata tcctcggcaa aatctcccgt aatcgtatgt acaccatggc
    37381 tgccacggca atgtccaccg tgtcgatgtc catcgttggt gcatacatga ccatgctgga
    37441 accgaaatac gtcgttgctg cgctggtact gaacatgttc agcaccttta tcgtgctgtc
    37501 gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac atccagatgt ccaacctgca
    37561 cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc
    37621 cattatcgtt gccgcgatgc tgatcggctt tatcgccctg atcgccgcgc tgaacgcact
    37681 gtttgccacc gttactggct ggtttggcta cagcatctcc ttccagggca tcctgggcta
    37741 catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac tgcaagtggg
    37801 cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa
    37861 aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc tggtttcctt
    37921 cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt aaaggcctga atgaagagca
    37981 aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc tggtgagtgt
    38041 gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat acataaaaaa gccggggatc
    38101 attccccggc ttttttatgc ctgttaatca gctaatggct gcgggcgacc aatcaaatac
    38161 ccttgctgat attgcacccc gagcttatgc aatagcgcct gctgctgttg cgtctcgaca
    38221 aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga tctcacaatc
    38281 atcgcatcca gcgtattcgt gacaatatct ttcacaaaga cgccatcaat tttgatgata
    38341 tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggcgccaaa gtcatcaatc
    38401 gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct ttctgcgtta
    38461 gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat gtgataacgc
    38521 ttaaacagac gaataatccg cccggcaata ttcttttgca gcagcgtgag cggcattaaa
    38581 ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca cttcaacaag
    38641 gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga
    38701 aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat ctcatcataa
    38761 ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc ctgatctaac
    38821 gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga aaccatctcc
    38881 tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc cagccagctt
    38941 aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc ccaggcagca
    39001 ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt caggatgtta
    39061 accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag cagttcacta
    39121 cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt
    39181 gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa ctcaagatta
    39241 tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    39301 cgaaagttgg gtaggagcgt taacggatct gttagcgcct gcaaatgcca gcggcgatta
    39361 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    39421 agcaccgcga gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
    39481 ttgtaattca gaaggcaaag cgttgaaaca gcctaggtga gatttaaaaa cggatagcga
    39541 agattaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    39601 aagtcgtttt cataaggtgt gcacaacaga agtaggagca cgcttaacgc tgccagccag
    39661 cttaaggtaa atgcgcgttt ctctttgccc aacgacggag cgatatccct gcgccacaat
    39721 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
    39781 atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg
    39841 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    39901 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    39961 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    40021 aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag
    40081 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    40141 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    40201 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    40261 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    40321 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    40381 caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct
    40441 aacccggacg taaactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt
    40501 tgcagcgagg gttg
//
LOCUS       DADLZM010000020        40384 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLZM010000020 DADLZM010000000
VERSION     DADLZM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.020"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..789)
                     /locus_tag="JNY73_001048"
     CDS             complement(<1..789)
                     /locus_tag="JNY73_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HAY8047137.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1036..1662
                     /locus_tag="JNY73_001049"
                     /pseudo
     CDS             1036..1662
                     /locus_tag="JNY73_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1659..1865)
                     /locus_tag="JNY73_001050"
     CDS             complement(1659..1865)
                     /locus_tag="JNY73_001050"
                     /inference="COORDINATES: protein motif:HMM:NF019607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY8047138.1"
                     /translation="MAAKIIYWALKDKDVIVMSVNGDKVTEDGRILTDRGQRIRDVRT
                     GPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1835..2773)
                     /locus_tag="JNY73_001051"
     CDS             complement(1835..2773)
                     /locus_tag="JNY73_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY8047139.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGR"
     gene            complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNY73_001052"
     CDS             complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNY73_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY8047140.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3480..4805
                     /gene="rimO"
                     /locus_tag="JNY73_001053"
     CDS             3480..4805
                     /gene="rimO"
                     /locus_tag="JNY73_001053"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY8047141.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4852..6180)
                     /locus_tag="JNY73_001054"
     CDS             complement(4852..6180)
                     /locus_tag="JNY73_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8047142.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVESINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6188..8536)
                     /locus_tag="JNY73_001055"
     CDS             complement(6188..8536)
                     /locus_tag="JNY73_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8047143.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFCVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8714..9625)
                     /gene="gsiD"
                     /locus_tag="JNY73_001056"
     CDS             complement(8714..9625)
                     /gene="gsiD"
                     /locus_tag="JNY73_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY8047144.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9628..10548)
                     /gene="gsiC"
                     /locus_tag="JNY73_001057"
     CDS             complement(9628..10548)
                     /gene="gsiC"
                     /locus_tag="JNY73_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY8047145.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10566..12104)
                     /gene="gsiB"
                     /locus_tag="JNY73_001058"
     CDS             complement(10566..12104)
                     /gene="gsiB"
                     /locus_tag="JNY73_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY8047146.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVIPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(12124..13995)
                     /gene="gsiA"
                     /locus_tag="JNY73_001059"
     CDS             complement(12124..13995)
                     /gene="gsiA"
                     /locus_tag="JNY73_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY8047147.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13982..14947)
                     /gene="iaaA"
                     /locus_tag="JNY73_001060"
     CDS             complement(13982..14947)
                     /gene="iaaA"
                     /locus_tag="JNY73_001060"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY8047148.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMESVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15151..16386
                     /gene="moeA"
                     /locus_tag="JNY73_001061"
     CDS             15151..16386
                     /gene="moeA"
                     /locus_tag="JNY73_001061"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY8047149.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16386..17135
                     /gene="moeB"
                     /locus_tag="JNY73_001062"
     CDS             16386..17135
                     /gene="moeB"
                     /locus_tag="JNY73_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY8047150.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17314..17976)
                     /gene="fsa"
                     /locus_tag="JNY73_001063"
     CDS             complement(17314..17976)
                     /gene="fsa"
                     /locus_tag="JNY73_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY8047151.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            18107..19006
                     /locus_tag="JNY73_001064"
     CDS             18107..19006
                     /locus_tag="JNY73_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY8047152.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19012..21444
                     /locus_tag="JNY73_001065"
     CDS             19012..21444
                     /locus_tag="JNY73_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY8047153.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21590..22405
                     /gene="ybiV"
                     /locus_tag="JNY73_001066"
     CDS             21590..22405
                     /gene="ybiV"
                     /locus_tag="JNY73_001066"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY8047154.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22557..23822
                     /locus_tag="JNY73_001067"
     CDS             22557..23822
                     /locus_tag="JNY73_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY8047155.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24063..25655)
                     /locus_tag="JNY73_001068"
     CDS             complement(24063..25655)
                     /locus_tag="JNY73_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY8047156.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25874..26794
                     /gene="ldtB"
                     /locus_tag="JNY73_001069"
     CDS             25874..26794
                     /gene="ldtB"
                     /locus_tag="JNY73_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8047157.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26852..27970)
                     /locus_tag="JNY73_001070"
     CDS             complement(26852..27970)
                     /locus_tag="JNY73_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY8047158.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFQTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27967..28434)
                     /locus_tag="JNY73_001071"
     CDS             complement(27967..28434)
                     /locus_tag="JNY73_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY8047159.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28620..28748
                     /gene="mntS"
                     /locus_tag="JNY73_001072"
     CDS             28620..28748
                     /gene="mntS"
                     /locus_tag="JNY73_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY8047160.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29020..30603
                     /locus_tag="JNY73_001073"
     CDS             29020..30603
                     /locus_tag="JNY73_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY8047161.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYKTFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30651..31166)
                     /gene="ompX"
                     /locus_tag="JNY73_001074"
     CDS             complement(30651..31166)
                     /gene="ompX"
                     /locus_tag="JNY73_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY8047162.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGLNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31219..31284)
                     /locus_tag="JNY73_001075"
     CDS             complement(31219..31284)
                     /locus_tag="JNY73_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047163.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31519..32406
                     /gene="rhtA"
                     /locus_tag="JNY73_001076"
     CDS             31519..32406
                     /gene="rhtA"
                     /locus_tag="JNY73_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY8047164.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32705..33208
                     /gene="dps"
                     /locus_tag="JNY73_001077"
                     /gene_synonym="pexB"
     CDS             32705..33208
                     /gene="dps"
                     /locus_tag="JNY73_001077"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY8047165.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33612..34358
                     /gene="glnH"
                     /locus_tag="JNY73_001078"
     CDS             33612..34358
                     /gene="glnH"
                     /locus_tag="JNY73_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY8047166.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            34497..35156
                     /gene="glnP"
                     /locus_tag="JNY73_001079"
     CDS             34497..35156
                     /gene="glnP"
                     /locus_tag="JNY73_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY8047167.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35153..35875
                     /gene="glnQ"
                     /locus_tag="JNY73_001080"
     CDS             35153..35875
                     /gene="glnQ"
                     /locus_tag="JNY73_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY8047168.1"
                     /translation="MIEFKNVSKHFGSTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36136..36330
                     /locus_tag="JNY73_001081"
     CDS             36136..36330
                     /locus_tag="JNY73_001081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047169.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTELAPEPDIEQ
                     KKRPMAHWRMCWITTPRVKS"
     gene            36297..38360
                     /gene="ybiO"
                     /locus_tag="JNY73_001082"
     CDS             36297..38360
                     /gene="ybiO"
                     /locus_tag="JNY73_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY8047170.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(38357..39283)
                     /gene="rlmF"
                     /locus_tag="JNY73_001083"
     CDS             complement(38357..39283)
                     /gene="rlmF"
                     /locus_tag="JNY73_001083"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY8047171.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39559..39819
                     /locus_tag="JNY73_001084"
     CDS             39559..39819
                     /locus_tag="JNY73_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8047172.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            40084..>40384
                     /locus_tag="JNY73_001085"
     CDS             40084..>40384
                     /locus_tag="JNY73_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY8047173.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10048 a        10679 c         9825 g         9832 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ctcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
      841 taagttcggt gattaataac gcacacacaa acttaaagaa accgtaagtt ggtaaagttt
      901 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
      961 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagtctgtg gctgcctgtc
     1021 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaactaa cgtaaaaaaa
     1081 gtattgctga cgctcgaata actggaacta ccttatgagc aaattctcgc gggccgtgag
     1141 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
     1201 ttacgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
     1261 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg
     1321 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
     1381 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
     1441 gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctct
     1501 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
     1561 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
     1621 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
     1681 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
     1741 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
     1801 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cccaataaat aatttttgct
     1861 gccactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg ggcgaatctt
     1921 tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct tccggtatct
     1981 taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt ttgccttttt
     2041 gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac agtgcattac
     2101 tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag atctcggcgc
     2161 gcgcaccgga ttcctttata aaaggattat catccgggat ttcgccctgg tcggtcagac
     2221 gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga ttgttttcgc
     2281 ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca aaatggttgc
     2341 cggtagacag ttttggcatc tggcggaaga cggtgcggaa gtcggtcact tttgagagat
     2401 catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg ccaacttcgg
     2461 aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc acgtccagca
     2521 ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca gataatcctt
     2581 ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata ccgtgattat
     2641 cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact tcgacattta
     2701 ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca aggaaaaagg
     2761 attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca gtggaggaga
     2821 taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc tcccccttag
     2881 ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca gcgttaagct
     2941 catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc aatcatggtg
     3001 cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat gaagagcact
     3061 ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt
     3121 cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt atccttttgc
     3181 cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag
     3241 atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc cttgttgcaa
     3301 ttaaatagta tggctatttt ttgagcaaaa tgctgtctag gatcaattct tcacgccgaa
     3361 ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat acaaatttta
     3421 tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt ttttaagcta
     3481 tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc
     3541 ttgtcgattc agagcgtatt ctcaccgaac tccgcactga aggttatgac gtggtaccga
     3601 gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac agcgcggtac
     3661 aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg
     3721 gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca
     3781 ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg ccaaaaccga
     3841 aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg ccgcgtcatt
     3901 atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc attattccgt
     3961 ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc
     4021 tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc gcctatggcg
     4081 ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc agcatggtca
     4141 gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac gtttaccctt
     4201 atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg ccgtatctgg
     4261 acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt ccgggttctg
     4321 tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac
     4381 gctcaacctt tattgtcggc ttccctggcg agacggaaga agatttccag atgctactcg
     4441 acttcctgaa agaagcacgt ctggatcgcg ttggctgctt taaatacagc ccggttgaag
     4501 gtgcagacgc caatgccctg cctgaccagg ttccggaaga agtgaaagaa gaacgctgga
     4561 accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag aaagtgggcc
     4621 gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg
     4681 cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac attaagccgg
     4741 gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg
     4801 tttaattaat tcaggccggg caaaatcatt tgtccggcct gcgaataatg attactgcca
     4861 gctaatctgt gccctgccgt ttttcttact cttatacagc cgctcatccg ccagcatttt
     4921 caagcgttcg acggtagtac atttatttga atgcgctaca ccgatactgg tgcttaaatt
     4981 aatgctttcc acctggaaat gaacgatttc ttttagtatc gatagcaact cttccagcgt
     5041 gccgccagta taaatcaccg caaattcgtc acctccgaag cggtagactt tacctttatc
     5101 taccgggatc tgtgatttaa ggattttcac cactttcatc aacacttcat cgcccagcaa
     5161 atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg tcaaacagac acaaataacc
     5221 gcctttttcc ggcagcgctt ttaaatcttc atcaaaggat tttctgccca ttgctttggt
     5281 caggtcgtcc aggaacgccg agttatacat actgtagagt tttttgacat cggcagccga
     5341 aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta ttaatgatgg cttctttctc
     5401 cacaatacca cgattaatca ctctgcgtac cagatagagg caactggcac ccgatatcac
     5461 tgccaccaat gcggcaaaga aaataatcaa tgtacaaacc cgcaaataaa catctttttt
     5521 atacgcaaat acgtacaatc cggtcggcaa ataggcgaag tgctccatcg cttccgaaac
     5581 ggtgaagatg gcgtttttag cattatattc atcaccggtc accggtttac cgttcttaaa
     5641 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
     5701 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
     5761 gaaggtttta ccatcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
     5821 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
     5881 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
     5941 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
     6001 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
     6061 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
     6121 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
     6181 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
     6241 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
     6301 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
     6361 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
     6421 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
     6481 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
     6541 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
     6601 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
     6661 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgttga ctaaaatgtc
     6721 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
     6781 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
     6841 ggcacaacgc ctccgcgctc agaatggcgc gggtgttaat atcaacgatc ggttgataga
     6901 cgaggaatag ttcgccaatt tccattgcat ggcgaatatt acgcgcaatc cagatattct
     6961 cttctatttc gttgagtttc gcgtcatcac agaaaataaa ctcggagaac atatggtcac
     7021 ggatactgga aatcgccagt ttcagttttt ctgcgtaact ccaggcgttt tccccctgca
     7081 aaggttcaca aatggcagac ttatggatgc gcagattgcg gtagccaaag ctgctgtcaa
     7141 aatcgtttac catcgctatt ttacgggtaa atgtctgtac attttcccga cacagaagta
     7201 aatagagatc ggcattgaat gtcacgatat cgcagcattg cgcatatttc tcggcaattc
     7261 gctgacacaa tttcctgacg attttattgc gttcatcaac acaaaacacc gcttccacgg
     7321 cttccagacc acacagtttg atgatcccca cagccagata ctgattattg ttggactcaa
     7381 taatcgccat atttttaata aaaccgcttt cctgatcgta agagatcagt gagagcaatt
     7441 tatttgaccg ttcaatttcg gcatctttca tttccgcgat cgaatttttg atgctgataa
     7501 tctctttcgc atcgccttcg cgaattttca gagcctcgat ctgatccgtt gagtcgggca
     7561 aaaacgccag cgtattaacc agctcggtca tttcagaaaa ttgccgacgg aacacacgag
     7621 tggtcatgaa atataccacc agcaacacga taataatgaa cgcaaacggt agataaaaaa
     7681 gtttatctga tgcgttcaat aaattaccgc caggataata aaaatagatt tcgaacaatg
     7741 cattatttgg cactgctgat gtctttgcaa tatactccgt atcgacagaa tcaataatat
     7801 tataattacc ttctctatta taaataatca gtttttcacg agcgagttga ttcttggcaa
     7861 tgatctcatg accttgtttc aaaaatagaa cttcatcatt agcgctgatt tgatcaagat
     7921 actcgttcag ataccgccag tttaaatcga catgaatttt atctaccgct aacaaatcgt
     7981 gatctttgcc gttgatacgt agcgtaaaag agatacattt tttacccgga cgatcaaagc
     8041 aattgtatgc cggattccag ctgttgttag agtcatctga agccaccgac cacggtcgtt
     8101 tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc ggtccattta
     8161 cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag agataatatt
     8221 tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc attttcagaa
     8281 tcacggcgcg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc tttaatgtca
     8341 gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata agttcaaagc
     8401 ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatc atgaaaaaaa
     8461 gaccaataaa tgacagtgct gccaataaaa ataaaattat cagccttatc tttatctttt
     8521 cgtataaact cagcatgtta atacatttaa caaaataact atctgaacgc tgtgccgctg
     8581 caaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca aaatcagcag
     8641 aaagttacaa cacttgctta gggcaataat ttcctgtgag gtgattaccc tttcaagcaa
     8701 tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca
     8761 acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg
     8821 gcgcgataac catatccgct cgtgcctcat tgagcattgc tccccactct ggtgtcggcg
     8881 gctgcgcacc taggccgaga aatgacaggc tggcggcaga gataatcgag gtgccaatgc
     8941 gcatggtgaa aaacaccacg atagaagaga cggtcccagg caggatatga cgcaataaaa
     9001 tggtcatatc gctggcacca atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca
     9061 ccagcgtgtt gccgcgcacc aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga
     9121 taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac
     9181 ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag ccgatcccac cagccttcat
     9241 aatatccagc caacaagccc agcaacgtcc ctatcgccac accgataaac acggcaaaca
     9301 cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca
     9361 gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt attcagattg tcataatcaa
     9421 aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc acaatcaata
     9481 aaataacgaa taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt
     9541 catgccacgg tgtacgtacc tggtcaggtt tgaccagtgg catggcgttt aacaccgcct
     9601 gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt taatggcggc
     9661 gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa gcagaatttc
     9721 cgcctgaatc accgggtaat cgcgcatttc tacagagtca acgagtaagc gtccaagtcc
     9781 gggccagttg aaaactttct ccaccacgat agaacctccg agcaaaaagc caaactgtaa
     9841 gcccatcatg gtcactaccg ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca
     9901 ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta aaacgtcgac
     9961 aaatgacgca cgggtaaagc gcgccatcac ggcggccact gccgcgccga gcgtcaggga
    10021 gggtaaaatg tagtgctgcc aactgtctgc tcccacagta ggcagccagc ccagttcaac
    10081 ggagaatacc tgaattaaaa acatccccag cgcaaatgcc ggaaacgaga tccccgacac
    10141 cgcaatggtc atactcaatc gatccggcca acgattacgc cagacggcgg cgataattcc
    10201 cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc acagcgttgg
    10261 cataaagcgg ctggcaatct catcggcaac cggacgacgc gacaccatcg acaggccaaa
    10321 atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg gctgatccaa
    10381 ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgggcc cggcaatcaa
    10441 tcgcgccgga tcgccgggca gcatgtggac aaataaaaac accagcaccg agacgataaa
    10501 cagcgtcgga atcaacccca gtaatcgttt gataacgtaa ttaagcattc cactccctgc
    10561 gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgc tgtctggcat gatccaaaaa
    10621 ccggtcaggt ttttactgtg tgccgacacc agtttttcta ccaccagcgg gatccacggc
    10681 gattcttgcc agatgatatc ctgcgccgcc ttatataagc gggtcttttc cgccggatca
    10741 ttcgttttca gtgcctgagc caggaagtca tccacctgtt tattgctgta aaacgcggta
    10801 ttaaacagcg tcggtggcca gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct
    10861 tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc tttttgccct
    10921 ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cggtcacctg ggctttaatc
    10981 ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta
    11041 tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt cagtaattcg
    11101 cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc gatacttggc
    11161 ggtatcacac cagtagctgg cgttgcatag cccgcaaagg caactttcac cagcgccgga
    11221 cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc
    11281 acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc gatatttttg
    11341 tttttctcca gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg
    11401 gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc
    11461 agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc
    11521 tgattccagg tatccagttc atacggtccg gttcccaccg gatgaaaacc aatctccttg
    11581 ccatattttt ccagcgctgt cggtgaaatc atcgcggtcg ccggatgggc aagaatatta
    11641 ataaacgctg agaacggctg tttgagggta atctttaccg ttgtcggatc gatcgcctcc
    11701 gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg atcgctggcc
    11761 cggtccagat tcgcttttac cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt
    11821 ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg aaacggtata actctccgcc
    11881 agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta aaacgatttc
    11941 gctacggcct gagataacgt gtcattcgcg tcatacggat cgagcgtggt gaaattcgat
    12001 cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa cgctgtcgca
    12061 atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt ctccgcctga
    12121 atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat gccccggccc
    12181 gacgcattgc aacgagacgg ctgccacctc ttcgccacgc agatgaatat tgctgggaag
    12241 atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc
    12301 cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc accgtgaacc
    12361 aatttcaaca atttgcccga gatacatcac cgccacacga tgactaatcc gctctaccac
    12421 agccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga gatcgagcaa
    12481 caagttgata atctgcccgc gaatagaaac atccagcgcc gaaacggctt cgtcggcaat
    12541 gatcactttt ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc
    12601 ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac aggcccacgc gctccagcaa
    12661 ccacgcaacg cgtgcagccg cgtctttacc tggcaataaa ccgtgtacac gcagcggttc
    12721 gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat
    12781 aaactgaata tcccggcgca atgcctgaag tttgccaggt gacaaggtat cgattcgctg
    12841 accgttaaag ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt
    12901 agtggattta ccgctgccag actcgcccac cagcgatagc gtttcgccag gccagagatc
    12961 aaaactgact ttctcaacgg catgcacttc ccgcgttacg cgattcaaca aaccgctgcg
    13021 caaagggaaa cgggtgacaa gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt
    13081 tttctgctcg atggggggtt cctgtttcgc tggatgttca agcgatatca atgggaaacg
    13141 tcggggataa tctaaccctt tcatcgcacc aagttgcgga acagcagcta acagcgcacg
    13201 ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc
    13261 gccctgatac atcaccagta cccgatcggc aatctctgcc accacgccca tatcgtgagt
    13321 gataaagata acgcccatcg acatctcttt ttgcaatact ttgattaatt gcaggatctg
    13381 cgcctgaata gtgacatcca gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg
    13441 gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga gttgatgcga
    13501 ataacgtgaa agaatggttt gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc
    13561 ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga cgaattgatt cggcaatctg
    13621 ttcacccaca gtaaatactg ggttcagcga tgtcatcggc tcctgaaata tcatcgccat
    13681 atccgcaccg cgcacatggc gcatttgtgc agcgctctgc tcgctaagtt caatcacatc
    13741 acgactgcgc cgccgcaaca gcattttatc gcactgtact aaaccgcccg cctgttccaa
    13801 cagacgcatc aatgccagcg cagtcactga cttaccggag ccggattcgc ctacaattgc
    13861 cagcgtctca ccgcgttgca gactaaaaga gagattgcgg accgcagcta ttttctgctg
    13921 gtcctgcata aaggcaatat taagattttc aaccgccagc acattaccgg catcaagttc
    13981 atcactgtgt ggcaacggtg tccccttttt cacggtagat accggtggtt ggcgtatcgc
    14041 ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg acattccctt
    14101 catggtcgat agcgattaag ccaccgctac cgccaagcgc agggagtttt tccattacta
    14161 cccgctcgca ggcttccgcg agacttaatc cgccgtaatc cattaacgcg gcgatgtcat
    14221 atgccgccag cgcgcggatg aagacttcgc ctgtgccggt acaagaaacc gccacactgg
    14281 cgttattggc gtagcatccg gcacccacta agggactatc gccaactcgt ccgggtaatt
    14341 tattggtcat tccgcctgtg gacgtggctg ccgccagatt gccgtctaaa tccaacgcca
    14401 cggcccccac ggtgcccatt ttctgttttt catccagtgg cgcaccgcta tggtcgagga
    14461 ctgttgcccc ttcctcgcgc gctgccagta gttgttcata acgcaaaggc gtggagaaaa
    14521 tctccggtga gacgctctcc atgccatgag caaacgcaaa attttctgcc ccttcgccaa
    14581 tcatcatcac atgcgggctt tgctccatca ccagccgggc ggcaagaacc ggattacgca
    14641 gatgactaac gcccgccacc gcaccggctt tcagggtgtt accatccatc acacaggcgt
    14701 ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta aacagtggac
    14761 actcttccag cagacgcacc gcttccgtca ccacatccag cgcactttcg ccggcttcca
    14821 gcattttctg cccggtttca acaatggcag acaacgcctc gatgtagcgt aattcctgtt
    14881 gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc atgaattgca atgactgctt
    14941 tgcccatagt attcgtccat taagggtggc tcgccacttt tgctataaat atcagaattt
    15001 ttatgaatta ctcattcaat ttttgagtat agaaagatgg atatggcatg taaaggcagg
    15061 agccaacgct acgcccgagg tggcatacag tctatggcgt ttttctgaca taataggcaa
    15121 attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt gatgtcgctc
    15181 gacaccgcgc ttaatgagat gctttctcgc gttaccccac tgaccaccca ggaaacgctg
    15241 ccactggttc agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc
    15301 ccggggtttg ataactccgc tatggacggc tacgcggtgc gtttagccga tattgcctcc
    15361 gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc agccatacca tggtgaatgg
    15421 cctgcgggta cctgcattcg tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg
    15481 gtggtgatgc aggagcagac tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg
    15541 cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc
    15601 ccggcgggaa ctcgcctgac taccgcagag ctgccagtaa ttgcttcact ggggattgcc
    15661 gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga tgaactccag
    15721 ttgcccggtc agccgctggg cgacggccaa atctacgaca ccaaccgtct cgccgtacac
    15781 ctgatgttag aacagctggg ttgcgaggta atcaacttag gaattatccg cgacgatcca
    15841 catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtagt gatcagttcc
    15901 ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga gctgggggag
    15961 atcgccttct ggaagctggc gattaaacca ggtaaaccgt tcgcattcgg taaactcagc
    16021 aatagctggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac cttctatcaa
    16081 ctggtacagc ctttgctggc aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc
    16141 cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc
    16201 ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg acatcagggt
    16261 tcacatatat ttagctcctt tagtctcggc aactgcttta tcgtgctgga acgcgatcgc
    16321 ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc
    16381 ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa atcattctgc
    16441 gcggttttga ttttgacggt caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc
    16501 tcggtggact cggctgcgca gcttcgcagt atctggcaag cgccggtgtc ggcaacctga
    16561 cgctgctcga cttcgacacg gtttcgctct cgaatctgca acgccagaca ctgcacagtg
    16621 atgccacggt cgggcaaccg aaggtggaat ccgcccgtga cgccctgacg cggatcaacc
    16681 cacatatcgc gattacgcca gtcaatgcac tgctagatga cgcagaactt gcagcattga
    16741 ttgctgaaca cgatctggtg ctcgactgta cggataacgt tgcggtacgt aatcaactga
    16801 acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag
    16861 ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt
    16921 tgtttggcga gaacgcgtta acctgcgtag aagcaggcgt gatggcaccg ttgatcggcg
    16981 taattggttc gttgcaggca atggaagcga tcaaactgct ggcaggttat ggcaaaccgg
    17041 ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa atgaaactga
    17101 tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc ctgataagac
    17161 gcgacaagcg tcgcatcagg catttgctcc agacgccaga tgcggcgtga acgccttatc
    17221 tgccctacgg ttcgggcaca gatttccaag cgcggcaggc atcggtgcac tccgccacgt
    17281 catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc caaacgctcc
    17341 ctgccagtcc tgctcaaact tcgccacagc ggcttccacc gccggatagc taatcatctg
    17401 ttgtgccaca tccagtggca gagtaattga ttcacatccg gccagtaagc agtccagcgc
    17461 ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat gcattttcaa
    17521 taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat caatacgatt
    17581 aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc
    17641 atataccgcg gttcccagcg tcggaatccc ttccgctttt aacatcttaa tcgctgccag
    17701 cccctcggct gtcaccggaa ctttcaccac gatatccgca ataatagaac gcagcttaag
    17761 cgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca gacgcccctg
    17821 accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggttttt tacccgcggc
    17881 gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca gcgccttcac
    17941 cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa tgtacttttt
    18001 gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat caatctaact
    18061 ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga ttttcaatat
    18121 tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc ttaaaggctg
    18181 ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct
    18241 gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg cgccggaagt
    18301 gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc cggagcatct
    18361 gacggcgtta accgactgct gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag
    18421 cgttgaggag atcatggcga ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg
    18481 cggcttaacg ctttcgggtg gtgaaccctt catgcagccg gaaatggcga cggcgctgct
    18541 gcaagcctgc cacgaggcag gcattcatac tgcggtagaa acctgtctac atgtgccgtg
    18601 gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt taaaacacgt
    18661 tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagggtgc tggataacct
    18721 gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga ttcagggctt
    18781 taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg agctgcacgt
    18841 tggcgaaatt cattttctgc cctaccacac gctgggcatc aacaaatatc acttacttaa
    18901 tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gagctgctcg attttgccca
    18961 gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac catgaccaca
    19021 ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt
    19081 gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta tcaacaacat
    19141 ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc
    19201 accatctgga tcaaacacta tgagttgatc attggcaacc aggcaagcga agttcgcgcc
    19261 gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga tgatctggca
    19321 gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg
    19381 tgcccgtggt ggcgcggtca gacagtacag gatcgctgct atggcatgtt taccgatgag
    19441 caaaaaggtc tgctggcgac cggcatcatt aaagcggaag gcaatatgac ctccggcgat
    19501 gcgcacctgg cggtcaattt cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag
    19561 aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa
    19621 cagttcctga aagcgattga tatcgtgctg gtggcagtca gtgaacacat tgaacgtttc
    19681 gctgccctgg cgcgtgcaat ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg
    19741 ctggcgatgg cagaaaactg cgatcttatc gcccaccagc cgccgcagac tttctggcag
    19801 gcgctgcaac tgtgttactt catccagttg attttgcaga ttgaatctaa cggtcactcg
    19861 gtgtcgtttg gtcgtatgga tcagtatctc tacccgtact atcgccgcga cgttgaactg
    19921 aaccagacgc tggatcgcga acacgccatc gaattgctgc atagctgctg gttgaaattg
    19981 ctggaagtga acaaaatccg ctccggctcc cactcaaaag cctctgcggg aagtccgctg
    20041 tatcagaacg tcaccattgg cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg
    20101 aatccactct cttacgcgat cctcgaatcc tgcggtcgcc tacgttccac tcagcctaac
    20161 ctcagcgtgc gttaccatgc aggaatgagt aacgatttcc tcgacgcctg cgtacaggtg
    20221 atccgctgcg gcttcgggat gccggcattc aacaacgacg aaatcgtgat cccggaattt
    20281 attaaactcg gtattgaacc gcaggacgct tatgactacg cagcgattgg ttgtatagaa
    20341 accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat caacttcgcc
    20401 cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg
    20461 ccacaagaaa aagcgttgtc ggcaggtaac ttcaacaact tcgatgaagt gatggccgcg
    20521 tgggatacgc aaatccgtta ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac
    20581 accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga tgactgtatt
    20641 gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc tggcctgcag
    20701 gtcggtattg ccaacctcgg caacagcctg gcggcagtga agaaactggt gtttgaacag
    20761 ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga tggcctgact
    20821 catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa cgacgatgat
    20881 accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact gaaacagtac
    20941 cataacccac gctacggtcg tggtccggtt ggcggcaact attacgcggg tacgtcgtct
    21001 atctccgcta acgtaccgtt tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc
    21061 cacaccccgc tggcagaagg cgctagtccg gcctctggta ctgaccatct cggccctact
    21121 gcggtcattg gctcagtggg taaactgcct acggcagcga ttctcggcgg cgtgttgctc
    21181 aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa actgatgatc
    21241 ctgctgcgta ccttctttga agtgcataaa ggctggcata ttcagtacaa catcgtttcc
    21301 cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt
    21361 gtcgcgggtt attccgcctt cttcaccgcg ctctctccag acgctcagga cgatatcatc
    21421 gcccgtactg aacatatgct gtaatctctt cggcccggct gcggcgtcgc cgggctaaat
    21481 cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg ctctgcgtca
    21541 cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta tgagcgtaaa
    21601 agttatcgtc acagatatgg acggtacttt tcttaacgac gccaaaacgt acaaccaacc
    21661 acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attaagttcg ttgtcgccag
    21721 cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg agatctcttt
    21781 tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc acggcgaact
    21841 gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc aactcaattt
    21901 tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc
    21961 actgatggca aaacactacc atcgcctgaa acctgtaaaa gattatcagg agattgacga
    22021 cgtactgttc aagttttcgc tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa
    22081 actacacata gcgctggatg gcattatgaa acccgtcacc agtggttttg gctttatcga
    22141 cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga aacgctggga
    22201 tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg agatgctgaa
    22261 aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg
    22321 ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga
    22381 taacacatcc ccttttaaca gctgacctct ctaccggagc catgcgctcc ggttttccct
    22441 tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt atttataact
    22501 tgtattgtca catgcaaatc agcatagttt cgaatgaaag ttaatgcgag gtggttatgg
    22561 cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc cgtcagatga
    22621 agcacgcgct gcgggcgcag cttggcgatg tccagcagat ctttaatcag ctaagcgatg
    22681 acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat gccgtctggc
    22741 cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag cgcgaacaga
    22801 ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa gcgctaggct
    22861 gggatcagtc gatgctggac tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc
    22921 ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac cccatctact
    22981 ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc
    23041 tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg
    23101 tcatctaccc tgaccgtatc cgccgccttc cgcccggaac gacctccaaa ggtcttggag
    23161 cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag cacgttttcg
    23221 ccaacgtctt taatggcaat ctggcgaaat acgatccctg gcatgcggca catcgtacgg
    23281 aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg acattccagg
    23341 gctggactgc gctctcggat atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc
    23401 ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac
    23461 tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat ccactgttaa
    23521 ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg tggcactgcg
    23581 acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca
    23641 ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac
    23701 tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag
    23761 gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttgggc atggatgttt
    23821 gagagttaat ggaagtgaat tgatgtggga ttaagaaaat cgattcgacg ttactcagat
    23881 tgctgacaag tgcgctttct taatgcctga tgcggcgtta acgccttatc cggcctacaa
    23941 agttctgcag attcaatata ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag
    24001 gctattttgc atttgtcatc agccgtcgtc accccgacag tatcggggtg acggataatc
    24061 acttactcga tccctttact acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg
    24121 cgttccgggg tgatttccag aatgcgggtc gccagggagc ttacgaactc acggtcgtgt
    24181 gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca tgttcagtga ctcgatggat
    24241 tccatatcca ggtggttggt cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt
    24301 aacttaccaa acagcatccg ccctttttcc ccaccggaaa gcactttagc tggctttttg
    24361 atgtcgtcct ggctgaacag caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg
    24421 ccttcctgct tccactgact catccattcg aacacggtca gatcattttc aaactcatat
    24481 tcgtggtcct gggcgtagta accaatgcgc gcgttttcag accattttac ggtgccgctg
    24541 tccggttgca gatcgcccac cagcgttttc agcagcgttg atttaccgac gccgttggta
    24601 cccagtaccg caagtttttc accgacctcc agcagcaggt tgagattttt aaacagtgga
    24661 ccgttatcaa accctttggt cagaccttcc acttccagcg cgttacggaa cagtttctta
    24721 tcctgttcaa aacggatgaa cgggttctga cggctggagg ctttcacctc ttccagtttg
    24781 attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta
    24841 aagcggctaa cgaaagattg caactcagca atctgcgctt tcttcttggc gttatcggcc
    24901 agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt acccggataa
    24961 acgcgcagct cgccgtaatc cagatccgcc atgtgggtac agaccatgtt aaggaagtga
    25021 cggtcgtgcg agatgatgat catggtgctg tcacgctcgt tcagcacctg ttccagccag
    25081 cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat atccggctca
    25141 gcaaacagcg cctgcgccag aagcacacgc agcttccagc caggagcaac ttcactcatc
    25201 gggccgtagt gctgttccac tggaattccc acgccaagca acagttcacc ggcgcgagct
    25261 tccgcagagt aaccgtccat ttcgccgtat ttcacttcca gatcggccac tttatagccg
    25321 tcttcttcac tcatttccgg caaagcatag atgcggtcgc gctcctgctt cacttcccac
    25381 aactctttat gccccatgat caccgtatcc agcacagtga acccttcaaa ggcaaactga
    25441 tcctgacgca gcttaccaat gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc
    25501 tcaaggtcgc cgccgagaat cttcataaag gtggatttac cactaccgtt cgcgccaatc
    25561 aggccgtaac ggttgccgcc gccgaatttg acggaaatgt tttcaaacaa cggcttactg
    25621 ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa agagatatga
    25681 caaaccgcgc attatgccat aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca
    25741 ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt gagctggaat
    25801 gaacttataa tgcgcttcca atactctaat attctcaacc caatggcctg ccaggcacaa
    25861 aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct tcgctgttgt
    25921 cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct
    25981 ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc tggagtattt
    26041 tgccgcggag taccagatgg gactttccaa tatgatggaa gcgaacccgg gtgtggatac
    26101 cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc tgccggatac
    26161 cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact atccgaaagg
    26221 gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag atacgcctat
    26281 caactggacc accaaagttg agcataagaa agcaggcccg acctggacgc cgaccgccaa
    26341 aatgcacgca gagtaccgcg ctgcgggcga accgctaccg gctgtcgttc ctgcaggtcc
    26401 ggataacccg atgggtctgt atgcactcta catcggtcgc ctgtatgcta tccatggcac
    26461 caacgccaac ttcggtatcg gcctgcgtgt aagtcatggt tgtgtgcgtc tacgtaacga
    26521 agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt ttattgatga
    26581 gccggtaaaa gcgaccaccg agccagacgg cagccgttat attgaagtcc ataacccgct
    26641 gtctaccacc gaagcccagt ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag
    26701 cgtgcagaca gtgaccggtc agccagatgt tgaccaggtt gttcttgatg aagcgatcaa
    26761 aaatcgttcc gggatgccgg ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga
    26821 ctcataatcc gcctttttta tttgccagac cttaccaggc cgggatcata actaacaaaa
    26881 cgtatcccac cagcgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc
    26941 agatgcggcg atcgttcgcc atacgtagcg caatcaaatt tgccagcgat cctggtaata
    27001 aaccaaagcc ccccacgttt accgcccatg ccagtaataa agacggaggt acataattga
    27061 gcagcaaaat ggtactcggc acgttactga tcacctgcga taaaccgact gccgttaacc
    27121 ataacccggg ttcagataaa tgactcacgt tacccaacac gccttgcaac gctggaagct
    27181 gggtcagtaa atggacgtcg ataaacatcg ccataaacac cagcagcaac gtccagtcca
    27241 cactcaacac cacgcgacgc gccagcagca caaagcccgc cgccacaatc accagtcccc
    27301 acagctcttg tttgaactcc agcgccgtca gaaaaacgat atacagcccc agacaattcc
    27361 acaccagccg cggtttccac tccggtgttt gcatccccgt atggtattgc agcgcctttc
    27421 cagggaaaca acaccagcac aacagcagga gtgtcagcat cattgcgcca gccagcggtg
    27481 ccatttgggc aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt
    27541 gcgggttgcc aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa
    27601 tcagccgatt aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa
    27661 taaacagcgc gacatcgttg gtctgaaagg tagaaagcag cgccgccgcc agcaccataa
    27721 acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa
    27781 aataaccgct taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg
    27841 tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc
    27901 cgataccaac aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag
    27961 gcaggctcat ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc
    28021 actaacatgg tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa
    28081 caccaacaag aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc
    28141 tccttctgcc gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc
    28201 cagccgctta agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc
    28261 cacctgacga gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc
    28321 aataagctcg cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac
    28381 gttcactaac tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct
    28441 tcctcattat ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag
    28501 ttgcgaaggt acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata
    28561 tctgtatgta atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta
    28621 tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa
    28681 tgctgctctc tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttcag
    28741 ataaatagcg gcgtcgcggt acgccgcttc actcctgctt ttatgcaggc ataacgcgtt
    28801 ttggtctgaa aaaaaccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc
    28861 gtcagtagtt gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg
    28921 gctcgcggat gcggacccct ttcccctctt cacgcactct tgcaggtatt gacccttgac
    28981 gccagggtaa gcacatggcg tttgttacca tagtggcata tgaatttaac cctcaaagaa
    29041 tcgcttgtta cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg
    29101 ctattaatta acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg
    29161 gctattttgc ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc
    29221 agttcgctgg tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc
    29281 aatacattgc tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt
    29341 tttccgtggt acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc
    29401 aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta
    29461 ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt
    29521 aaactgaaag atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat
    29581 aacgaggtga ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg
    29641 acggtcactg ccgttaagcc gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg
    29701 cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc
    29761 gttaacggtt tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt
    29821 ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc
    29881 ctagcaaatc gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa
    29941 tacgatactg ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa
    30001 ggtaattttg aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa
    30061 gtgctggcgc aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat
    30121 ccgcaggcct gcgacaggac acaaggcaaa tacaaaacct ttgtgcaatc gaaagaaacg
    30181 tcgtgctatc tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag
    30241 ttacgcaaca gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt
    30301 aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc
    30361 caggtgcctt ttatggtcat ttccagcggc gataaagcgc atcgggtgat taaagcccgc
    30421 cgctcagcca atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa
    30481 ataaatatca aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac
    30541 ttccagttac agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct
    30601 taaaaacaaa aatccgcccc gagaggcgga tttttatatc accaaagtga ttagaagcgg
    30661 taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa
    30721 gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc gtaggagaaa
    30781 ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa tttaccataa
    30841 cccacaccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca
    30901 gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg gcttttctcg
    30961 gtgtaagtga aagaaccgat cacacccagc gggctgttgt cttcttcata gcggtatttc
    31021 aggtttaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca
    31081 gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac tgcggccagt
    31141 gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat aagtcctaag
    31201 ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca
    31261 tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat cactttttca
    31321 gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta ttcctaatca
    31381 ccagactaat gattccatca atcctggcgc attttagtca aaacggggga aaattttttc
    31441 aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac ttaagtctag
    31501 atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg gttaccaata
    31561 gtcatattac tcgttgccat ggcgtctatt cagggtggag cctcgttagc taagtcactt
    31621 tttcctctgg tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg aacgctgatc
    31681 ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg gttaccgctg
    31741 ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctttc tattcagaca
    31801 gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt ggcgctgttc
    31861 tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc
    31921 ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc gctggcactg
    31981 ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg agcggaacat
    32041 ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt gccaattgga
    32101 gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg tctggctgtc
    32161 gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac ccgtttgcca
    32221 acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt ttccgggatg
    32281 attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc tatcatcgcc
    32341 gcttcaatgg ggtctacgct gacagtacgc aaagagagca aaataaaaga attagacatt
    32401 aattaaattt acatttctgt atggttatgc ataaccatgc agaatttctc gctacttttc
    32461 ctctacaccg tctttatata tcgaattatg caaaagcata tttattccga aaattcctag
    32521 cgagcagata aataagaatt gttcttatca atatatctaa ctcattgaat ctttattagt
    32581 tttgtttttc acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca
    32641 catagccggt gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga
    32701 aattatgagt accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa
    32761 cgatgtctcc gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca
    32821 gtttattgat ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt
    32881 cattgccgta catgaaatgc tggatggctt ccgcaccgca ctgatcgatc atctggatac
    32941 catggcagaa cgtgccgtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa
    33001 cagcaaaacc ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa
    33061 agaactggct gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc
    33121 gaaagatgac gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct
    33181 gtggtttatc gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ctttagtacc
    33241 cgataaaagc ggcttcctga caggaggccg ttttgttttg cagcccacct caacgcactt
    33301 atttagtgca tccatctgct atccccagct gattaagtaa attttttgta tccacatcat
    33361 cacacaaccg ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg
    33421 ttgagtgcac aattttagcg caccagattg gtgccccaga atggtgcatc ttcagggtat
    33481 tgctctataa atcgtgcatc acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa
    33541 ctggcacgat tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa
    33601 aaggaaatgc tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg
    33661 cggtttcttc tcatgccgcg gataaaaaat tagttgtcgc gacggatacc gccttcgttc
    33721 cgtttgaatt taaacagggc gataaatatg tgggctttga cgttgatctg tgggctgcca
    33781 tcgctaaaga gctgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc
    33841 cggcactgca aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc
    33901 gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga
    33961 aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga
    34021 agagcggtac tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc
    34081 agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc
    34141 tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag
    34201 cggtaggtga ctctctggaa gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg
    34261 agctgcgtga caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg
    34321 aagtctacaa aaaatggttc agtactgaac cgaaataata acgctacacc tgtaaagcgc
    34381 actggcagtt ccctctcccc cctggggaga ggattagggt gaggggcgca aacccgctcc
    34441 ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc
    34501 agtttgactg gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga
    34561 ccctgtggat ttcggtcctc ggtctggctg gcggtctggt aatcggattg ctggcaggtt
    34621 ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga
    34681 tccgcggcac acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt
    34741 ttaacgactt acgcatcgac ccatttactg cggcggtggt caccatcatg atcaactccg
    34801 gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg
    34861 aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc
    34921 tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca
    34981 cctcactgtt tattgttatc ggcgtggcgg aactgacccg tcaggggcaa gaaattattg
    35041 ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta
    35101 ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga
    35161 atttaaaaac gtctccaagc actttggctc aacccaggtg ctgcacaata tcgatttgaa
    35221 cattgcccag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct
    35281 gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct
    35341 gaaggttaac gatccgaaag ttgacgagcg cctgattcgc caggaagcag gtatggtgtt
    35401 ccagcagttt tacctcttcc cgcatctgac ggcgctggga aacgtcatgt ttggtccgct
    35461 acgcgtgcgt ggcgcgaaca aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa
    35521 agtcggtctg gcagaacgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca
    35581 gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc
    35641 gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc
    35701 tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc
    35761 ttcgcggctg atctttatcg acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt
    35821 gatcaagaac ccgccgagcc agcgcttgca ggaatttttg cagcacgtct cttaataaga
    35881 cacattgcct gatgcgctac gcttatcagg cctacaggat atctggcaac ttattaaaat
    35941 tgcatgaact tgtaggacgg ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg
    36001 ttgtcagcaa ccggcttaat atcccttcct ccctttcacc cgaaagggag gcacaccaga
    36061 ttcctctcat ttaaaatcgc ccctccccca gcatctatac ttatcttttt gctctgtttt
    36121 ctcactggag gagtcatgcg gtggatcctg ttcatcctct tctgcctgct gggcgcacct
    36181 gcccacgcgg tatccatacc cggcgttaca accacaacga caacggactc aacgactgaa
    36241 ctggccccgg aaccggatat cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc
    36301 tggataacga cacctcgcgt aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc
    36361 cccctgcgga accggtcccg aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc
    36421 tgcaaaaggt caccgaagtc agccgccatt atggtgaagc cctttccgcc cgcttcgggc
    36481 aactttatcg caatatcacc ggctccccgc ataagccatt taatccacaa accttcagca
    36541 atgcgctgag ccatttttca atgttagcgg tattagtgtt tggtttttac tggctgattc
    36601 gcctgtgcgc actgccgcta tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg
    36661 agcgcagcaa ctggttgcag cttccggcga tgattattgg ggcgtttatt atcgacctgc
    36721 tgttactggc cctgacatta tttgtcggcc aggtattaag cgacaacctg aatgcaggta
    36781 gtcgcaccat cgctttccaa caaagtttgt ttctcaacgc ctttgccctg attgaatttt
    36841 tcaaagccgt actacgcctg attttttgcc caaacgtggc ggagctgcgc ccgttcacga
    36901 ttcaggacga gagcgcccgt tactggagtc gccgcctgag ctggttaagc agcctgattg
    36961 gctatggcct gattgtggcc gtgccgatta tctctaatca ggtgaatgta cagataggtg
    37021 cgctggcgaa cgtcatcatt atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc
    37081 gaaataaaaa agagattacc cagcatttgc tcaactttgc ggagcattcg ctggcttttt
    37141 tcagcctgtt tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc gcctatttta
    37201 tcgtgctgtt tttcttttcg ttgttcgatc cgggcaacag tctgaaattt atgatgggtg
    37261 caacggtgcg cagcctggcg attattggta tcgcagcgtt tgtttccggt atgttttccc
    37321 gctggctggc gaaaaccatc accctctcgc cacatactca gcgtaactat ccggagctac
    37381 aaaaacggct gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct
    37441 gcgtggcagt aatgttgctg ttgagcgcat ggggattgtt cgatttctgg aactggctgc
    37501 aaaacggcgc ggggcagaaa accgtagata tcctgatccg catcgcactc attcttttct
    37561 tctcggcggt tggctggacg gtgctcgcca gtttgatcga aaaccggctg gcttcggata
    37621 ttcatggccg cccgctaccc agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg
    37681 cgctggcggt gattatcagt accatcacca tcatgattgt gttgtcggaa atcggcgtca
    37741 atatcgcgcc attgctggca ggtgccgggg cattaggtct ggctatctcg tttggttcgc
    37801 aaacgctggt aaaagatatt atcaccgggg tatttattca gtttgaaaac ggcatgaaca
    37861 ctggagattt ggtgactatc gggccgttga ccggcacagt ggaacggatg tcgattcgct
    37921 ccgtgggcgt gcgccaggat accggggcgt atcacatcat tccgtggtct tcgataacca
    37981 cctttgctaa cttcgtccgc ggtattggtt cggtagtggc aaactacgat gttgatcgcc
    38041 atgaagatgc tgataaagcc aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa
    38101 acgaagaaat tcgcgggctg attattggtg aaccgaactt tgccgggatt gtcggcttaa
    38161 gcaataccgc gtttacactg cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg
    38221 tacgctttgc cctcgacagc caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc
    38281 cagtgcagac ttatcaggtg ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc
    38341 cgggggagcc aacgctttaa cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca
    38401 taaaggtcca ggcaataaag cgactctgct tttgcccctg ggccatctct ttttttacca
    38461 ccttcaccgc gcccacgtcg gtcagagcac gatacaacgg cggtaagttt tcaccacgag
    38521 ataccagcga ggtaaaccac atcacctgct tcgcgaagcc tttgctctct tcaatcatct
    38581 ttttgataaa gttgacttca ccgccttcac accacaactc ctgttgctga ccaccaaagt
    38641 tcagtgcatc gtctttgttc agccccaggt tacggcgttt acgctcacta cctgcccgcg
    38701 ccgcagcggc ggaatcgtgg aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt
    38761 tgtggatgat gccgttaaaa atcgccccac tctctttttg ccgacgcaga cgaatggcgc
    38821 ggttaagccc cggattagca ctgataatcg cctgcgcact gcttaacgcc tggctgctgg
    38881 tttcactacc ggtaaaacgc cagccatatt catgtacgcc aatcagcgga taaatacagt
    38941 tcgcaccaac gccgatatcc agaatgctgg cattcgctgg aattgttccg cttgcttcag
    39001 ccagtaaatc ggcaaggtga tgaatataat ccgcccggcc cggtactggt gggcagagaa
    39061 aaccgtcggg gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac gccttattga
    39121 gcgccttcac cgccagcgga ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg
    39181 tgaggaattg cctgagttca ggattgacct gacaaagcgt ggcgagatcg tagcggctgt
    39241 gatgacggtt gcgcggatgc aaccccggtt tctgggcgga catagcattc tcctttaagc
    39301 agcggcgtaa gatacccgca taacgcgaca gggtaaatat ctggctgcgt ttatcattcc
    39361 ccaaaacctt aacatggcta tggtttatgg caattcatca taaaaatcag cattatgcat
    39421 gtcattgatt tgtattattt tgtaaaaata tatcgttcaa aaaataagca tatctctgtc
    39481 aattttttgc gtcatttgca tcgcgcaggc gacctacact tatcaggcac tacccgttgt
    39541 atttatgagg atgtcattat gaaaaagtgc ctcacactac tgattgccac cgtcctgagc
    39601 ggaatctctc tcacggctta tgccgcgcaa ccgatgagta acctggacag cggtcagcta
    39661 cggcccgccg gtaccgtttc ggcaactggc gcatcaaacc taagcgatct ggaggataaa
    39721 ctggcagaaa aagcgcgcga acaaggcgcg aaaggttatg tcattaattc cgcaggcgga
    39781 aatgaccaga tgttcggtac tgcaaccatc tacaaataac cccaccaacg taactgcatt
    39841 cgcctctgga tgcagttttc gcctgttttg taatcaaaaa acatcaaata tgaacgcaat
    39901 gtaaataaat gtatttcttt ttcgcgcaat gggtgataga aaatcgcgtc aagtgataat
    39961 acttatcaaa attattatca cttgcacaaa cactatcacg ggattaacag tggcatcgca
    40021 tccgcagaga tgctttctcg tggcagtgaa aatttcaaca tataagaaaa agtcacctgc
    40081 aaaatggaaa acaatcgcaa tttccctgcc agacaatttc attcgctcac gttctttgcc
    40141 ggtctttgta ttggtattac gcctgtggca caggcactcg ccgccgaagg gcaagctaac
    40201 gcggatgaca cgctggttgt cgaagcatca acgccttcgc tttatgcgcc acaacaatct
    40261 gccgatccga aattctcgcg tccggtagcg gatactaccc gcacgatgac ggtgatttct
    40321 gaacaagtga ttaaagatca gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc
    40381 ggcg
//
LOCUS       DADLZM010000021        40019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLZM010000021 DADLZM010000000
VERSION     DADLZM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.021"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(26..280)
                     /locus_tag="JNY73_001086"
     CDS             complement(26..280)
                     /locus_tag="JNY73_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY8047174.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="JNY73_001087"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="JNY73_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY8047175.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="JNY73_001088"
     CDS             1230..2696
                     /locus_tag="JNY73_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY8047176.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2814..3800
                     /locus_tag="JNY73_001089"
     CDS             2814..3800
                     /locus_tag="JNY73_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY8047177.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="JNY73_001090"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="JNY73_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY8047178.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="JNY73_001091"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="JNY73_001091"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY8047179.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="JNY73_001092"
     CDS             5711..7267
                     /locus_tag="JNY73_001092"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY8047180.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="JNY73_001093"
     CDS             7349..9163
                     /locus_tag="JNY73_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8047181.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="JNY73_001094"
     CDS             9164..10258
                     /locus_tag="JNY73_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY8047182.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="JNY73_001095"
     CDS             10258..11283
                     /locus_tag="JNY73_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8047183.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="JNY73_001096"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="JNY73_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY8047184.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="JNY73_001097"
     CDS             complement(12878..13222)
                     /locus_tag="JNY73_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047185.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="JNY73_001098"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="JNY73_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY8047186.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="JNY73_001099"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="JNY73_001099"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY8047187.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="JNY73_001100"
     CDS             15618..17378
                     /locus_tag="JNY73_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY8047188.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKDGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="JNY73_001101"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="JNY73_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY8047189.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEINVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="JNY73_001102"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="JNY73_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY8047190.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="JNY73_001103"
     CDS             19115..19342
                     /locus_tag="JNY73_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY8047191.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="JNY73_001104"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="JNY73_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY8047192.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="JNY73_001105"
     tRNA            21197..21273
                     /locus_tag="JNY73_001105"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="JNY73_001106"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="JNY73_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY8047193.1"
                     /translation="MHQSCSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVDKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="JNY73_001107"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="JNY73_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY8047194.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /locus_tag="JNY73_001108"
     CDS             complement(25145..26197)
                     /locus_tag="JNY73_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY8047195.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDTNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="JNY73_001109"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="JNY73_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY8047196.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /locus_tag="JNY73_001110"
     CDS             complement(26748..28691)
                     /locus_tag="JNY73_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY8047197.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="JNY73_001111"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="JNY73_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY8047198.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="JNY73_001112"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="JNY73_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY8047199.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="JNY73_001113"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="JNY73_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY8047200.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="JNY73_001114"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="JNY73_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY8047201.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="JNY73_001115"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="JNY73_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY8047202.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="JNY73_001116"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="JNY73_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY8047203.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="JNY73_001117"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="JNY73_001117"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY8047204.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="JNY73_001118"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="JNY73_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY8047205.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="JNY73_001119"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="JNY73_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY8047206.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="JNY73_001120"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="JNY73_001120"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY8047207.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="JNY73_001121"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="JNY73_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY8047208.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="JNY73_001122"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="JNY73_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY8047209.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37393..37557
                     /locus_tag="JNY73_001123"
     CDS             37393..37557
                     /locus_tag="JNY73_001123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047210.1"
                     /translation="MHTPIGVKPAAGSKEWREAWQKRAFAHISNVYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="JNY73_001124"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="JNY73_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY8047211.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38330..39361)
                     /locus_tag="JNY73_001125"
     CDS             complement(38330..39361)
                     /locus_tag="JNY73_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HAY8047212.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFELVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39595..39954)
                     /locus_tag="JNY73_001126"
                     /pseudo
     CDS             complement(39595..39954)
                     /locus_tag="JNY73_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
BASE COUNT         9741 a        10828 c         9888 g         9562 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggccca tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagctcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcaa tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggccaggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca cttggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcgcccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg catttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct aggtatcgat acctggtggt atgacgttaa
     9121 taaagcggca aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cgtcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 caccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat
    13861 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccctttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaagacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgaccccc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg ctccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac
    17701 ggtaaagaaa tcaacgttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga caccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcttggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttat ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgctttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaacaagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcaacaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tggtatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaggt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtacctc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccaaggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg acggttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcgggcgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg cagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atgtttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
    38341 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
    38401 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
    38461 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
    38521 tagcctgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agattcaaca
    38581 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt
    38641 gctggtcact gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct
    38701 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
    38761 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
    38821 gatatttcac cagctcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
    38881 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
    38941 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg
    39001 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat
    39061 taaccacgtc tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
    39121 ctttcgcttc cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga
    39181 taaagacaaa tttggtgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
    39241 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
    39301 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca
    39361 catcagtgcc aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
    39421 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
    39481 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
    39541 aacgatattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
    39601 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
    39661 gccatcggcg ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc
    39721 gttgttccag ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat
    39781 cgaccattca ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc
    39841 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
    39901 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
    39961 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acggtaaca
//
LOCUS       DADLZM010000022        39556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLZM010000022 DADLZM010000000
VERSION     DADLZM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.022"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(72..1325)
                     /locus_tag="JNY73_001127"
     CDS             complement(72..1325)
                     /locus_tag="JNY73_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY8047213.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="JNY73_001128"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="JNY73_001128"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY8047214.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="JNY73_001129"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="JNY73_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY8047215.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMLL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="JNY73_001130"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="JNY73_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY8047216.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="JNY73_001131"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="JNY73_001131"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY8047217.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="JNY73_001132"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="JNY73_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY8047218.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPLWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15079)
                     /locus_tag="JNY73_001133"
     CDS             complement(14756..15079)
                     /locus_tag="JNY73_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY8047219.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15064..15471)
                     /locus_tag="JNY73_001134"
     CDS             complement(15064..15471)
                     /locus_tag="JNY73_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY8047220.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15468..16031)
                     /locus_tag="JNY73_001135"
     CDS             complement(15468..16031)
                     /locus_tag="JNY73_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY8047221.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16022..16492)
                     /locus_tag="JNY73_001136"
                     /pseudo
     CDS             complement(16022..16492)
                     /locus_tag="JNY73_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16676..17470)
                     /gene="thyA"
                     /locus_tag="JNY73_001137"
     CDS             complement(16676..17470)
                     /gene="thyA"
                     /locus_tag="JNY73_001137"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY8047222.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17477..18352)
                     /gene="lgt"
                     /locus_tag="JNY73_001138"
     CDS             complement(17477..18352)
                     /gene="lgt"
                     /locus_tag="JNY73_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY8047223.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18503..20749)
                     /gene="ptsP"
                     /locus_tag="JNY73_001139"
     CDS             complement(18503..20749)
                     /gene="ptsP"
                     /locus_tag="JNY73_001139"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8047224.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSI
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20762..21292)
                     /gene="rppH"
                     /locus_tag="JNY73_001140"
     CDS             complement(20762..21292)
                     /gene="rppH"
                     /locus_tag="JNY73_001140"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY8047225.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21977..22666
                     /gene="mutH"
                     /locus_tag="JNY73_001141"
     CDS             21977..22666
                     /gene="mutH"
                     /locus_tag="JNY73_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY8047226.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22735..23448
                     /locus_tag="JNY73_001142"
     CDS             22735..23448
                     /locus_tag="JNY73_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY8047227.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23586..23804
                     /gene="ygdR"
                     /locus_tag="JNY73_001143"
     CDS             23586..23804
                     /gene="ygdR"
                     /locus_tag="JNY73_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY8047228.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23912..24952
                     /locus_tag="JNY73_001144"
     CDS             23912..24952
                     /locus_tag="JNY73_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY8047229.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="JNY73_001145"
     CDS             complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="JNY73_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY8047230.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FCPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACVLAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26171..28330)
                     /gene="aas"
                     /locus_tag="JNY73_001146"
     CDS             complement(26171..28330)
                     /gene="aas"
                     /locus_tag="JNY73_001146"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY8047231.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSVITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28719..29750
                     /gene="galR"
                     /locus_tag="JNY73_001147"
     CDS             28719..29750
                     /gene="galR"
                     /locus_tag="JNY73_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY8047232.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29757..31019)
                     /gene="lysA"
                     /locus_tag="JNY73_001148"
     CDS             complement(29757..31019)
                     /gene="lysA"
                     /locus_tag="JNY73_001148"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY8047233.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31141..32076
                     /gene="lysR"
                     /locus_tag="JNY73_001149"
     CDS             31141..32076
                     /gene="lysR"
                     /locus_tag="JNY73_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY8047234.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32063..32755)
                     /gene="ygeA"
                     /locus_tag="JNY73_001150"
     CDS             complement(32063..32755)
                     /gene="ygeA"
                     /locus_tag="JNY73_001150"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY8047235.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(32884..34297)
                     /locus_tag="JNY73_001151"
                     /pseudo
     CDS             complement(32884..34297)
                     /locus_tag="JNY73_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(34612..35373)
                     /gene="kduD"
                     /locus_tag="JNY73_001152"
     CDS             complement(34612..35373)
                     /gene="kduD"
                     /locus_tag="JNY73_001152"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY8047236.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35403..36239)
                     /gene="kduI"
                     /locus_tag="JNY73_001153"
     CDS             complement(35403..36239)
                     /gene="kduI"
                     /locus_tag="JNY73_001153"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY8047237.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(36559..37707)
                     /locus_tag="JNY73_001154"
     CDS             complement(36559..37707)
                     /locus_tag="JNY73_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY8047238.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            37962..38120
                     /locus_tag="JNY73_001155"
     CDS             37962..38120
                     /locus_tag="JNY73_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter"
                     /protein_id="HAY8047239.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTY"
     gene            38319..39191
                     /locus_tag="JNY73_001156"
     CDS             38319..39191
                     /locus_tag="JNY73_001156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8047240.1"
                     /translation="MVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPLIAY
                     FLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDRREK
                     YGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAKEEGVTILSALSMLP
                     NAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNHALSI
                     MLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGNLIT
                     LIVGILCVSVMFFS"
BASE COUNT         9800 a        10721 c         9602 g         9433 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaaa gcgccattca
       61 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
      121 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
      181 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
      241 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat ttttccgagc
      301 ttattcagta ccgctttacc aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata
      361 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
      421 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag
      481 agcgcaaaca ccgaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
      541 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
      601 atgtcttcat tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc
      661 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
      721 ccggaatctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
      781 gctttacccg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
      841 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
      901 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
      961 tttacgtttt gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg
     1021 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     1081 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
     1141 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     1201 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
     1261 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     1321 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     1381 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     1441 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     1501 cgaaaaaaca gaataaaaat acaataattt caaataatca tgcaaagagg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactaga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcaatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gtatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggagcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacagcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagtgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg tcgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgctc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcacctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaagcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcagtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cgtttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcactgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcggata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cagcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta cggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatattaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gggttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagagcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    14941 actggtttct gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat
    15061 tcttcaggga agctgacata atgccccctc tttcagcgga caaaaatcgc tccagccgcg
    15121 tggcgaaaaa acaatttttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc
    15181 ataaccggca atcaataagg cttcattatc tgccagtaaa cgcaaacaaa cctgggcatc
    15241 ggttccagcg tactgcgagc actgaactgc tggctgcgtc tgccaggagt gcatttttcc
    15301 ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac
    15361 gcgagaggca aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac
    15421 cagcagcatc aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat
    15481 cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc
    15541 atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    15601 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    15661 gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct
    15721 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag
    15781 tcaccctgtc cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc
    15841 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagtgccagc
    15901 ttacgagtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc
    15961 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    16021 tttacaggca tccttctgtt tctccttgct gataaagccg gagtcttccc cacggcgaaa
    16081 ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat
    16141 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc
    16201 gtggcacaaa gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca
    16261 caaggcacca taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt
    16321 tggcatcttc acgtaaatag agcaaatagt cccgcgccta gctggcggtt tgccaaagcc
    16381 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa
    16441 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    16501 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    16561 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac
    16621 ggagggtaaa aaaaccgacg cacactggcg tcggctctgg cagtatgttt cgtaattaga
    16681 tagccaccgg cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga
    16741 aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg
    16801 gttcgcggct taattgcaga tgagtttgat ccatatggtt gctgtacagg tgcgtatcgc
    16861 caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc atatgcacca
    16921 gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag gagcgctgat
    16981 aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca tggcacggtg
    17041 ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat
    17101 ccgggtcgtt tttcagctgg ttcagtaccg tagtaatctg gtcaatatga cgaccatctg
    17161 gcgttggcca ggcgcgccac tgtttaccat acactggccc gaggtcgcca ttttcatcgg
    17221 cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta gtatcgccct
    17281 gcaggaacca cagcagttcg tggatgatgg aacgcaggtg gcaacgtttg gttgtcacca
    17341 gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg
    17401 ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat
    17461 actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca
    17521 tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact
    17581 gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa
    17641 acgcgccgta gccaatcagg aacaagcctg agacagctcc cattgggcgc ggtttacgaa
    17701 tatacaggtt gaggataata aacagcacca caccttccag cagcagttcg taaagctgtg
    17761 atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt
    17821 gcagcagcaa aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg
    17881 ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac
    17941 caaacggaat gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc
    18001 gggcgaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc
    18061 cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca
    18121 gaacataacc aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt
    18181 caacttcatt tttggtccag ccactgcccg gacgattcgc ccgacgtgtt gccagccaca
    18241 ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta
    18301 ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc
    18361 acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca
    18421 ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta
    18481 aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac
    18541 gctccataaa ggctgcaacc tgatgacgaa cttcggtcgc cagttgcgct tccagactac
    18601 gctgcgcaag attttctgct tcggcaaaat caatgcgccg cagcaggtat tttacccgcg
    18661 ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc
    18721 acatgggatc gcccgccatt tcaccgcaca aacggagatc gataccatgt atttccgctt
    18781 cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtcg taaatgttcg
    18841 ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa
    18901 cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt
    18961 ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac
    19021 gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctg gtgaccatcg
    19081 gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga
    19141 tcaagaagat ctccggctga tcgagcgtaa tgcgaatacc acgccaaccc aggcatggat
    19201 tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca
    19261 aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt
    19321 cttccgacgg gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc
    19381 catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga
    19441 cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac
    19501 ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg
    19561 gatcgaccag caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg
    19621 aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag
    19681 catgggagtt ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct
    19741 cagcaagagt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt
    19801 tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat
    19861 cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt
    19921 caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt
    19981 cgcgacgcag ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt
    20041 ctttttgtgc tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt
    20101 ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca
    20161 cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg
    20221 ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacagggca gtcaactgcg
    20281 actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt
    20341 cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt
    20401 gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttatggag gggatgtatt
    20461 tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca
    20521 ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc
    20581 cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga
    20641 cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc
    20701 gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga
    20761 cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca
    20821 gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg
    20881 acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg
    20941 tactgctggt ttgcatattg atttctgcat cgccgcttac cagctgcaag agaaaccatt
    21001 tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt
    21061 tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta
    21121 cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc
    21181 cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc
    21241 gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac
    21301 ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac
    21361 tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta
    21421 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
    21481 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat
    21541 tttaaaatat tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa
    21601 ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat
    21661 ttatccacag aatgtgccac taagttaagc actgaaccac taaaaactgg agtttcgtcg
    21721 cacgtcaagg ctgtaaatga aaacagtagt ggaggttttt cacagttatc ccagctttct
    21781 gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacctattg
    21841 cagtgttgcg caactcgatt accggcagcc taaaaagcaa tataaatcag agaattgaac
    21901 aacacatgtg gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta
    21961 accaaggcat catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga
    22021 acagttgtta gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact
    22081 tgccgggctg gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact
    22141 ggagatctgg ctaggtgcca gcgcagggag taaacctgag caagattttg ctgctctggg
    22201 cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt
    22261 ttgtgttgcc ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca
    22321 caagctcaaa cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca
    22381 gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg
    22441 cgaagactgg gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc
    22501 tcgtcacggg gaatatttac agataagacc gaaagcagcg aatgcgaaag cgcttaccga
    22561 agccattggt gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa
    22621 gaatttcacc agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc
    22681 tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta
    22741 tttgcatgga taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag
    22801 atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca
    22861 gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg
    22921 ctgctggcat caatctcttg ggttacgcgc ctgacaaatc cgctttttac aatattcagt
    22981 caggaaattt ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa
    23041 gccagcaagg aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt
    23101 tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt cagcctcgac
    23161 tcggtgatta ccgctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg
    23221 attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat
    23281 ccttcggtaa aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg
    23341 gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt
    23401 gcggttgaaa gcctcaactt gattcgcaac aaaaagaatc cgctctgata ctccgtacgc
    23461 tctcctgcgg gagggcgtac tcactccttt taccctcaca gtttaagatt ttcctgcttt
    23521 caagatatat aacgtcgatt tataaacaga ctattatcat aggtaggcca aatgaggata
    23581 accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg
    23641 ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa
    23701 aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga
    23761 tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat
    23821 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
    23881 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
    23941 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
    24001 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
    24061 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
    24121 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
    24181 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
    24241 ccgcgaagcg ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca
    24301 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
    24361 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
    24421 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
    24481 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
    24541 gttaaaccgc agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact
    24601 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
    24661 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
    24721 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggccttg atcctgctca
    24781 gatggcgctc gcgtttgttc gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
    24841 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
    24901 attagctgaa attgaagcgg tacatcaggt ttatacttat cctgcaccat aaagattaac
    24961 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    25021 tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
    25081 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    25141 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    25201 gagcggaacg acaaaaaagc cccccagcac gccaatcagc atcaacaagg cataggctgg
    25261 cagcagctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa tcccggctgg
    25321 catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac
    25381 gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag
    25441 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    25501 actggtgccc accagcgaaa aacgcgtttc accattgcac catagcgagg tgcaggcatt
    25561 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    25621 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtacgc atgcggccag
    25681 ggcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gtaaaatcgc
    25741 cgctatggca gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    25801 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    25861 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    25921 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    25981 cgccaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    26041 acagaactgc gctttcagta acgccagagt ggcaaacagt agcgcgttat cgccaaacgc
    26101 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    26161 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    26221 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
    26281 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    26341 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    26401 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    26461 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    26521 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    26581 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    26641 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    26701 ggcgtgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    26761 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    26821 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    26881 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    26941 tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac caagtccggc acaatgcggt
    27001 aatgcagcgg gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc
    27061 ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg
    27121 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
    27181 cgacgccttt tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
    27241 gctgctgttt tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt
    27301 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    27361 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    27421 tgatttcagc cgccgtaata acactggtca gcccttttac cccggcagtg tagttcatca
    27481 ttgcgggaat gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    27541 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    27601 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    27661 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt
    27721 cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    27781 ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa
    27841 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    27901 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    27961 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
    28021 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
    28081 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    28141 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    28201 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    28261 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    28321 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    28381 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    28441 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    28501 ctgggatcac cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg
    28561 tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg
    28621 gctcacattc ccacgatgaa aacacgccac cccttgaacc aacgggcgtt ttccgtaaca
    28681 ctgaaacaat gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag
    28741 cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag
    28801 ccagcgaagc ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga
    28861 acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg atcgttggtg
    28921 atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca
    28981 ccggtaattt cttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcta
    29041 ttgagcaact gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccgg
    29101 atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc
    29161 tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc tggctggcaa
    29221 cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta
    29281 tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattc
    29341 cggccaatga tcggctggtg acatttggcg aaccagacga aaacggcggc gaacaggcaa
    29401 tgaccgagct tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa
    29461 tggcggcggg cgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt
    29521 cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg
    29581 tgcgttaccc aatcgtaacg atggcgaccc aggctgccga actggctttg gcactggcgg
    29641 ataatcgccc tctcccggaa attactaatg tatttagtcc gacgctggta cgtcgtcatt
    29701 cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa
    29761 agcaattcca gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga
    29821 ccattatcaa acagaacttc tggtaacagc ggacggctat tgtagttgga tgacattgat
    29881 gcgccatatg cccctgtatc atgcagtacc agataatcac ctgccttcac ttccggcaag
    29941 gcgcgggttt caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac
    30001 ggcccggcga cgacggtttc caccgttggc gcgtgttcca gagaacgacc atcagctgcc
    30061 agggcactga tatggtggta actaccgtac attgccgggc gcatcagatc gttgaacccg
    30121 gcatcaacca gcacaaagtg gcggctaccc atttgtttga cgctccgcac ctgagtaatt
    30181 aatacgccag actgcgctac caggaagcga cccggttcaa tttccagttt cacagggtgg
    30241 cccaaatggc gagcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta
    30301 tcaaccgcct cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc
    30361 tgtaaatcct gaccgaattc gatgacctga cgcaccatag caccacacac ctgctccaga
    30421 tgggcataat caacgccaga accaatgtgc atgtgaatgc cgactagctg cagatgatga
    30481 cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt
    30541 tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa accccggatt aacgcgcagc
    30601 catacccgat gccctggcga aacctggccc agttggtcga gcatatcaac agaacccgca
    30661 ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc
    30721 gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct
    30781 atttcgccta acgagacgga atccactttc acgccctgct cacgcattaa gcgcaaaata
    30841 tgaatattgg aacaggcttt ctgtgcaaag cgcaccactt caaactgttt cagcgctgca
    30901 atctgccgac gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc
    30961 aaacgcagca gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata
    31021 acaaactcca gatgagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata
    31081 tcgatttatg tcgaatctat gcaaaaatga tatggattac cggattgcga gagagcgcta
    31141 atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc
    31201 ctgactgagg cagcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg
    31261 cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg attacatcct
    31321 accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc
    31381 attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc
    31441 ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat
    31501 cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg
    31561 gcccagcgtc atgatttagg actcactgag acactccata cgcctgcggg aacagaacgt
    31621 accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta
    31681 aaaaaggtat taacgccgga tgattttcag ggtgagaatt acatcagcct ttcccgtact
    31741 gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg
    31801 atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtgtt
    31861 tcggtggtta acccgctcac cgcactagat tatgcggcaa gcggtttagt ggtgcggcgg
    31921 ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca
    31981 gcgctggttc aggcgtttag tgggcattta caagcggggt taccgaaact ggtcacttct
    32041 cttgacgcta ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga
    32101 tcgccgcggt atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt
    32161 ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg
    32221 cataataagc gcgtgacgct tcggtaaatt tccccagaca cagttcttca aaaataatct
    32281 gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg
    32341 tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac
    32401 gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca
    32461 gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa
    32521 taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt
    32581 tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca
    32641 gcatttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat
    32701 agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc
    32761 tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg
    32821 ccaggtgccc aatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg
    32881 aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc
    32941 cagcgtgaca tttttggttt ccagaatgag ccagaaagta atgcccacaa acgcaatgtt
    33001 caacgcagtg tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa
    33061 ggccgcgccg ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg taataccgaa
    33121 atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct
    33181 catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact
    33241 ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc
    33301 catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac
    33361 cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg
    33421 ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca tgatgatgtt
    33481 cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac
    33541 gttacggttg atcttaaaca gtgcccaacc gccctgtttt aacttcaggc tttcacgaat
    33601 ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc
    33661 cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac
    33721 cagaataatc agcagaactg ctggtaaagc aagaacccca acattgcgcg ccagttaccg
    33781 ctataactga acgctgtatc ggataaaaac gccagcacga tgccgagtgt gaccatcaac
    33841 tggtacatac tgatcatctt accgcgaacg tacttgccat ttcagaaaga tacagaggag
    33901 cggtgtaaga cgcgatcccg acagcaatgc ccagcaccac acgagcggcg attaacatct
    33961 ctacgctggt cgcaaaagcg gaccctatag aaccgagtac aaacaggatg gcccccgcca
    34021 tcaggctgta tttacgcccc aggcggaacg acagccaacc attaaacagc gcaccaattg
    34081 ctgcgccgag catcatgcta ctaaccaccc attcctgcaa acgactggtc agcacaaagt
    34141 gatcggtaat gaacggcaac gctccggcga ttacgccgat atcaagacca aataacaatc
    34201 ctgcgaccgc agcagctacc gaaacaaaca tattcatacg ccgcgtatca cgcaaagaac
    34261 gtggcgttaa agcagattcc gtattgatag taaccatttt ttcctgccag cagagagtaa
    34321 gacattgtga aaaaatacgt gaacaattca cacaggtgtc aggtcggaaa cagcataaat
    34381 atggattaaa ttgctgcgac atgccgttat gtgatggata ttccaatttt aaaattaagt
    34441 tgaattattg agattattat taaccaccta attttaaagt agataaaatt cataaagttc
    34501 attaattgat aattgatatg gattatttca taaccatgat atggattatg atgatctaga
    34561 gatataaaaa accctgccat gcggcagggt cataaaagta agaggaatga attaacgcgc
    34621 cagccaaccg ccatccacag caatggtata accatttaca taatctgaag cgctggaggc
    34681 aagaaacact accggcccca tcaggtcact cggcagtccc caacggccag ctggaatgcg
    34741 atcgagaatt tccgcgctac gttgttcatc tgcccgcagt tgttgggtat tgttggtcgc
    34801 catgtaaccc ggagctatcg cattaacatt aatgttgtgt ttagcccatt cgtttgccat
    34861 caatcgcgtc acacccatca ctccgctttt tgatgcggta taagaaggca cacggatccc
    34921 gccctggaag gagagcattg acgcgatatt gataatcttg ccgccattgc cttgcgcgat
    34981 aaagtgtttc gccgctgcct gagacatgaa gaatacgctc ttgatattca ggttcatgac
    35041 atcgtcccag tctttttcgc tgaactcgag agcgtcttcg cggcgaatca atccggcgtt
    35101 attcaccagg atatcaatat gaccaaattc agctaccgcg cgatccagca gtccaggaat
    35161 gccatcaatc tttcgcagat cggcggtcag gcttaaaaaa cgacgcccca gcgccgtgac
    35221 ctgctcgatg gtttcagtcg gttcaacgat gttaatgcca acaatgtcac agcccgcttg
    35281 cgccagcccc aacgccatcc cctggcccag cccagtatca caaccagtga cgaccgcaac
    35341 tttaccttgg agagaaaatg cacttaaaat cataacaaca ccttattcgt ttatgcccac
    35401 aactagcgca aatctttaac ggccacatgg tccatatcat taaagacctg gttttcaccg
    35461 accatgcccc agataaaggt ataagctttg gttccgacgc cggaatggat cgaccagctc
    35521 ggggagatca ccgcctgctc gttatgcatc acaatgtgac gcgtttcttg cggctgcccc
    35581 atcatgtgga aaacgcaggc gtcatcatcc atattgaaat agaaataaac ttccatccgg
    35641 cgctcgtggg tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc
    35701 cccatactca attgacaggt ttccagtaca tccgggacaa aatatttgtt aatcgtgcga
    35761 cggttactgg tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt
    35821 ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc cggagtggcg
    35881 gtatcaatac tggcaaagac aacttctttt gcacctttac caacatacag ggcgtcgcgg
    35941 tgaccgattt catagcattg accatcgaca gtaatcgtac cggcaccgcc aatattgata
    36001 acacctaact cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg
    36061 ccaacggaaa ccgttttagt tatcggcata atgccgccaa caataattcg gtcaatgtgg
    36121 ctgtaaacca tggtgtactc atcggcgaca aatacctttt caaccaaaaa ttcattacgc
    36181 agcccttggg tatccagcgt ttttgcgtgc gcaccgtgga tgctctgtct tacgtccaca
    36241 tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac actgtttcgt
    36301 tttctctata aagggatgat atgcccatgg gctagggttt tcaattaaaa ataaaacaat
    36361 gttttatttt taatttaagg attaaaaaaa atgcagtgtc gatcacgaat atgtcatgag
    36421 aatggaaaaa tgagggagcc cagaaaatgt atgtcgactt gccagccatc attattcaac
    36481 agattaatga cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc
    36541 aatggtcaat gccacgccct agcccccgcc gatacaaagc gttgccagcc ctttgcgggc
    36601 attacgtttc accatttcat gaaccagaga aaccaggatt cggcaaccgg aagcgcctat
    36661 cgggtgaccg agtgcgatcg cgccaccatt gacattgacc cgacgctcat cccactcaag
    36721 catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac
    36781 atcagccaac tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat
    36841 tcccatcaat gccggatcta caccaacgct ggcaaatgcg cggatgcggg ccagcacggg
    36901 taaattcaac gctcgtgctt tggcttcgct catcatcatt accgcagctg cgccatcgtt
    36961 tatggatgat gcattccccg cggtcacaga accgagacta tcaaatgaag gatttaaacg
    37021 ggctaaacct tctgcactgg cgtcagtgcg tggctgttca tcggtatcaa caaccaacgt
    37081 ctgaccgtta ctttggatca ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat
    37141 cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat
    37201 gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc
    37261 ccacaaccca tcatgcacca gactgtcaac caactggcta ttgccaagct gtgcaccggt
    37321 gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac
    37381 aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagaccgga
    37441 accgcaaacg tcattaatcg tgattgcaga aacgctgtta ggaagaccac ctttaatagc
    37501 cgattgcctt gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc
    37561 atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttcacga ccagactacc
    37621 aagttccacg gcggaatgac ccgctaacgc accacgaaag cagccgatag gtgtccgtaa
    37681 cgccccgaca atcacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta
    37741 aatgattgtt aattacagtt atcttaattg tttaaaaaaa gtgattttta tcacaaagga
    37801 aatatgcctg agcagtagtc agagtcataa ctggcacgta aggtttgcaa ccactaaccc
    37861 accaatagat gggtagatag ggcgttaatc tcccatactt aacttggttt atggtaaatt
    37921 gccctccatt ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcta
    37981 aagaagaaac tctgtggagt ttcgcgctct acggcacagc cgttggtgca ggcacgctat
    38041 tccttcctat tcagttaggt tcggcagggg ctgtggtcct gtttattact gctctggtcg
    38101 cctggccttt aacatattag ctacataaag ccttatgcca gttcatcctc tcatcaaaaa
    38161 catcagcagg tgaagggata acgggcgcgg taacacacta ctatggcaag aagattggta
    38221 atctgattac cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg
    38281 caattaccaa ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc
    38341 gtatgttggt gagtctgggt gttgtattaa ttctgaatct catttttctg atgggacgtc
    38401 atgccactat tcgggtaatg ggatttttgg tattcccatt gattgcctat ttcttatttc
    38461 tttccattta ccttgtcggt agttggcaac ctgatctatt aacaacccag gtagagttca
    38521 atcagaatac ccttcaccag atatggatat cgattcccgt gatggttttc gcctttagcc
    38581 atacgcccat tatttctacg tttgccatag acagacgtga aaaatatggc gaacacgcta
    38641 tggataaatg caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtactgt
    38701 tctttgtctt tagctgcctg ctttctattc catcttcgta tattgaagcg gctaaagaag
    38761 aaggggtcac cattttatcg gcgctttcta tgctgccgaa cgccccagca tggttgtcaa
    38821 tttccgggat tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg
    38881 gcgttattga aggtgccaca gaggtcgtca aaacaacact acagcaggtt ggtgtaaaga
    38941 aaagtcgtgc atttaaccac gcactatcaa ttatgttggt atcgctgatt accttcattg
    39001 tttgttgcat taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg
    39061 ccatgatact tttcatcatg cctacgctgt caacgtatct catcccggcg cttaaaccct
    39121 ggcgttccat cggaaatctg attacgctga tcgtgggtat cctgtgcgta tcggtaatgt
    39181 tctttagcta atatccgttg tggatggttc aaaattgcta caattttgaa ccattccatt
    39241 tttcataata ttcatttgtt attattccgc cctatcactt ctctcgtcat atcctagaat
    39301 tatcagttag ttaaacacac aaagagatct gaacatctta aataagacta tttaagatgc
    39361 ataacttaga ttcgcaagat atcttctggt cattcaaaaa caatttgcga tatagatatt
    39421 ctcataccat gagatagtta tctggaccac gttcgaccat gcaagattaa atcagtgaaa
    39481 cataatcata tttaataacg agaaatgcat ttttaaatat cttaatcaga attatagcaa
    39541 atacagatta atcgca
//
LOCUS       DADLZM010000023        37626 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLZM010000023 DADLZM010000000
VERSION     DADLZM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.023"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..175)
                     /locus_tag="JNY73_001157"
     CDS             complement(<1..175)
                     /locus_tag="JNY73_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY8047241.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQYR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="JNY73_001158"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="JNY73_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY8047242.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="JNY73_001159"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="JNY73_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY8047243.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="JNY73_001160"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="JNY73_001160"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY8047244.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="JNY73_001161"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="JNY73_001161"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY8047245.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNAVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="JNY73_001162"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="JNY73_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY8047246.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="JNY73_001163"
     CDS             complement(5179..5421)
                     /locus_tag="JNY73_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY8047247.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="JNY73_001164"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="JNY73_001164"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY8047248.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNTIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="JNY73_001165"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="JNY73_001165"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY8047249.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="JNY73_001166"
     CDS             complement(8338..9312)
                     /locus_tag="JNY73_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8047250.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="JNY73_001167"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="JNY73_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY8047251.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVIEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /locus_tag="JNY73_001168"
     CDS             11709..12752
                     /locus_tag="JNY73_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY8047252.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="JNY73_001169"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="JNY73_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY8047253.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="JNY73_001170"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="JNY73_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY8047254.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="JNY73_001171"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="JNY73_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY8047255.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="JNY73_001172"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="JNY73_001172"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY8047256.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /locus_tag="JNY73_001173"
     CDS             16538..20338
                     /locus_tag="JNY73_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY8047257.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="JNY73_001174"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="JNY73_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY8047258.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="JNY73_001175"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="JNY73_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY8047259.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="JNY73_001176"
     CDS             23179..23382
                     /locus_tag="JNY73_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY8047260.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="JNY73_001177"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="JNY73_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY8047261.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="JNY73_001178"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="JNY73_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY8047262.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGMLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="JNY73_001179"
     CDS             26387..26659
                     /locus_tag="JNY73_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047263.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="JNY73_001180"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="JNY73_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY8047264.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="JNY73_001181"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="JNY73_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY8047265.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="JNY73_001182"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="JNY73_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY8047266.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="JNY73_001183"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="JNY73_001183"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY8047267.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31773)
                     /gene="degQ"
                     /locus_tag="JNY73_001184"
     CDS             complement(30406..31773)
                     /gene="degQ"
                     /locus_tag="JNY73_001184"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY8047268.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31927..32325)
                     /locus_tag="JNY73_001185"
     CDS             complement(31927..32325)
                     /locus_tag="JNY73_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY8047269.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32519..33646
                     /locus_tag="JNY73_001186"
     CDS             32519..33646
                     /locus_tag="JNY73_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY8047270.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33865..34293
                     /gene="rplM"
                     /locus_tag="JNY73_001187"
     CDS             33865..34293
                     /gene="rplM"
                     /locus_tag="JNY73_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY8047271.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34309..34701
                     /gene="rpsI"
                     /locus_tag="JNY73_001188"
     CDS             34309..34701
                     /gene="rpsI"
                     /locus_tag="JNY73_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY8047272.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35096..35734
                     /gene="sspA"
                     /locus_tag="JNY73_001189"
     CDS             35096..35734
                     /gene="sspA"
                     /locus_tag="JNY73_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY8047273.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35740..36237
                     /gene="sspB"
                     /locus_tag="JNY73_001190"
     CDS             35740..36237
                     /gene="sspB"
                     /locus_tag="JNY73_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY8047274.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36280..37542)
                     /gene="dcuC"
                     /locus_tag="JNY73_001191"
     CDS             complement(36280..37542)
                     /gene="dcuC"
                     /locus_tag="JNY73_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY8047275.1"
                     /translation="MMCGVWLGFGGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLS
                     IMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPYIILSATYVIGQIMAQFITSASGL
                     GMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVAGMKIATYFFH
                     YQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLI
                     LMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGT
                     QFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMG
                     SGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVS
                     PFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9109 a         9412 c         9904 g         9201 t
ORIGIN      
        1 ttctgtactg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 accaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atagatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaagggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccctatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagc gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggttaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cggcattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcggttt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gctaggtgca taccgaacat caggattgcg actacaatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcatctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagtgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taactttgta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgcgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaagataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtgcttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat acggctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgactttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatagaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgt gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggagccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgcc
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcata
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgcc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttgacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag agcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc gggcagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacatt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaagct cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgtcga gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggatgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcctaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg acgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactcta accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gatgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt aatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa agcccccgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtacaca ctaattattg
    27061 aactaattaa taccacgcta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcgaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggcgt
    30661 gatcatttca gcgctggccg acgaagaggt gctggtatca agcgtcactt ctacttccag
    30721 tggtttgccg ttacgcagca ggccaagctt cactttcgtg cccggctctg tagtcgcgat
    30781 acgagagcgc aactcagcaa agctattcag cggtttgccg ttgaggctgg taataatatc
    30841 gcccgctttg acgcccgctt tcgctgagcc agaacttggc aacacttcgc tgacaaacgc
    30901 gccacgctgc acgtcaaggt tgaaggcttt ggcgatatcg gcactcatct cggtgccttt
    30961 gatgcctaac aaaccgcgtt tgatttcacc aaagtcgata agctgctgcg ccagtgttcg
    31021 cgccatatta ctggggatgg caaatccaat cccgacgctc ccgccgccag gcgcaaggat
    31081 tgcagtgttg atgccaatta actcaccgtt aaggtttaat agtgcaccgc cggagttacc
    31141 gcggttaatg gaagcatctg tctggataaa gttttccaga ccttcaagat tcaacccgct
    31201 gcggcctaat gcggaaacaa tgccagaggt ggcggtttgc ccaaggccaa atgggttacc
    31261 gaccgctacg gcaaaatcac cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt
    31321 taatttgctc gggttttgaa tttgtaacag ggcgatatcg ctctggtcat cgctaccaat
    31381 cagttttgca tcaaactcgc gcccatcatt gagctgaata ctgattttct gtgcctgatt
    31441 aatcacatgg ttgttggtca gcacatagcc tttactggcg ttgatgatga caccggaacc
    31501 taaaccttcg aagggttgtg caggttgatc cggtaaatca tcaccaaaaa actttttgaa
    31561 ttcttccggg attttctgtc cctgactggc cgttccttcc acccgtacgc tcaccactgc
    31621 cggaagcact ttttccagca ttggagccag actggggaga ggggcctgat cggcaacctg
    31681 gcctggaatc gacgcgacgg cctgaaatga cgccgagaga gttaacccgg cacttaacgc
    31741 taatgcactc aacagctggg tttgtttttt cattattcct gctctcgtac ctgaatgata
    31801 agaaaagaga ttcaaaacag tttgatgtta ttgaattttc agacggctaa caatgagaca
    31861 ccagattaaa tgatagtcgg ggagggagag aagaggaggg cgcaatggct gcgcccgaaa
    31921 aataaattag tcgcgcttcg cgccagtacg cagcaggccg gatgcgcctt cagaatagtc
    31981 gcgaggcatc tgcaccggtg cctgatcgtt gctggcttca gactctgcca gacgattacg
    32041 gaacgggttt gcttcagcag acagttccgg cagcaggctg ctggagcttt ttgccatgtg
    32101 ctgatacagc tggcgatagt cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa
    32161 gtggctaacc agctcttcgc gatactcgtc cagttcagct ttattctttt ccagttcgta
    32221 ctgcaacgcc tgttgctggc gtagtttacg attaccaaaa cgcatggcca cagcaccaat
    32281 aatgatgccg acgactaacc caattagcgc atattcccag gtcatgaaca tctcccgttg
    32341 tcttttgttt ccgtagggtg ttggcttcag gctccatgcc tgcggctgat tatgccacta
    32401 taaccgttaa ttccacagaa gtggaatccc gactgcatat cgcgtagtgt agaacggcct
    32461 ttttttcgtc aaccgtgaac aacggcgcac cgattattca aggaataaca ataagatcat
    32521 gcaaagcgtt accccaacat cgcaatacct gaaggcgctc aatgaaggca gccatcaacc
    32581 cgacgacgtt caaaaagagg ccgtcagccg cctggaaatt atttatcaag aactcatcaa
    32641 tagcaggcca ccagccccca ggacgagtgg gctaatggcg cgggtcggta agctgtgggg
    32701 taaacgcgaa gacacaaagc atacgccagt gcgtggctta tatatgtggg gcggtgtagg
    32761 acgcgggaaa acctggctga tggacctttt ctatcaaagc ctgccgggag agcggaaaca
    32821 gcgcctgcac tttcaccgtt ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg
    32881 gcagaccgat ccgctggaaa ttattgccga tcgctttaaa gccgaaactg acgtgctctg
    32941 ttttgacgaa ttttttgttt ctgatattac cgacgccatg ctacttggcg gtctgatgaa
    33001 agccctgttc acccgcggta ttaccctggt agcgacgtca aatattccgc cggacgaact
    33061 ttatcgaaat ggcctgcaac gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca
    33121 ttgtgatgta atgaacgtgg acgctggtgt tgattatcga ctgcgtacac tcactcaggc
    33181 gcatctgtgg ctttcgccac tcaacgatga aacccgggcg cagatggata aactatggtt
    33241 ggcgctggcg ggggtgaaac gagaaaattc accgacgtta gaaatcaacc atcggccatt
    33301 ggcgacaatg ggcgtcgaga accagacgct ggcggtttct tttactacgc tgtgcgtcga
    33361 cgcccgcagt cagcatgact atattgcgct ctcacgcctg tttcacacag taatgttgtt
    33421 tgatgtgcca gtcatgacgc ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt
    33481 ggatgagttt tacgagcgcc atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga
    33541 aatttatcag ggtgagcggc tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga
    33601 gatgcaaagc gaagagtatc tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat
    33661 cacaaaaagg ggtcgatctt tgaccccgac ttctctataa tcctgcgacc ccacgttaca
    33721 agaaagtttt tttcccaaaa ctttttgtgt gctggcatag gctattcgaa ggggtaggtt
    33781 tgccggactt tgtcgtgtga acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt
    33841 aactatttat tgggtaagct tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa
    33901 cgcgactggt atgttgttga cgcgaccggt aaaactctgg gccgtctggc tactgaactg
    33961 gctcgtcgcc tgcgtggtaa gcacaaagcg gaatatactc cgcacgtaga taccggtgat
    34021 tacatcatcg ttctgaacgc tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa
    34081 gtgtactatc accacaccgg ccacatcggt ggtatcaaac aagcgacctt tgaagagatg
    34141 attgctcgcc gtcctgagcg tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc
    34201 ccgctgggtc gtgctatgtt ccgtaaactg aaagtttacg cgggtaacga gcacaaccac
    34261 gcggcacagc aaccgcaagt tcttgacatc taatcgggat tataggcaat ggctgaaaat
    34321 caatactacg gcactggtcg ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc
    34381 aacggtaaaa tcgtaatcaa ccaacgttct ctggaacagt acttcggtcg tgaaactgcc
    34441 cgcatggtag ttcgtcagcc gctggaactg gtcgacatgg ttgagaaact ggacctgtac
    34501 atcaccgtta aaggtggtgg tatctctggt caggctggtg cgatccgtca cggtatcacc
    34561 cgcgctctga tggaatacga cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt
    34621 actcgtgacg ctcgtcaggt tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt
    34681 ccgcagttct ccaaacgtta attggcttct gctccggcag aaaacaattt gcgaaaaaac
    34741 ccgcttcggc gggttttttt atggctaaat tctgaatcag cgtaaaaaat gggaagttgc
    34801 tttttgctgc cgtctgaaag acaggtaaaa caaaaaccac atcgccaata agggtctaag
    34861 tcaactattt cagactaaag cgcatctctt tttccccatt tccggcatcc actcaccaca
    34921 aaggtcgcaa aatctggtaa actatcatcc aattttctgc ccaaatgtcg ggtattgctc
    34981 attttttgtt tgattttcga acaaagagag tcgttcctta ttgggtaaca caacttctga
    35041 ctggccacct ggtggctggt agcagtaaaa attctgacta tacctggagg ttttcatggc
    35101 tgtcgctgcc aacaaacgtt cggtaatgac gctgttttcc ggtcctactg acatctatag
    35161 ccatcaggtc cgcattgtgc tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga
    35221 aaaggacaat ccgcctcagg atctgattga cctcaacccg aatcagagcg ttccgaccct
    35281 ggtggatcgt gagctgaccc tgtgggaatc tcgcatcatt atggaatatc tggatgagcg
    35341 tttcccgcat ccgccactga tgcctgttta cccggtagct cgcggtgaaa gccgtctgta
    35401 catgcatcgc atcgaaaaag actggtacac gctgataaac actatcatca acggttcagc
    35461 ttctgaagca gatgccgcac gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt
    35521 cttcggtcag aagccgtact tcctgagcga tgagttcagc ctggtcgatt gctaccttgc
    35581 tccgctgctg tggcgtctgc cgcaactggg catcgagttc agcggcccgg gtgcgaaaga
    35641 gctgaaaggc tatatgaccc gcgtctttga gcgtgactct ttccttgctt ctttaactga
    35701 agcagaacgt gaaatgcgtc tgggccggag ttaatctgta tggatttgtc acagctaaca
    35761 ccacgtcgtc cctatctgct gcgtgcattc tatgagtggt tgctggataa ccagctcacg
    35821 ccgcacctgg tggtggatgt gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt
    35881 gacgggcaaa tcgtactcaa cattgcgccg cgtgctgtcg gcaatctgga actggcgaat
    35941 gatgaggtgc gctttaacgc gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg
    36001 gctgccgtgc tggctatcta cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa
    36061 gctgcctacg atgaagatac cagcatcatg aatgatgaag aggcatcggc agacaacgaa
    36121 accgttatgt cggttattga tggcgacaag ccagatcacg atgatgacac tcatcctgac
    36181 gatgaacctc cgcagccacc acgcggtggt cgaccggcat tacgcgttgt gaagtaatac
    36241 aaaacaggcc caggcggcct gttttgtctt tttaatgact taataaaata gcgtgattgt
    36301 ggcaatcata ttcaccacga aaccgactgc catggggatc gctgtccgct tcaccaccgc
    36361 aaaaggcgaa acgcctgcaa ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac
    36421 cgcgcgcgct agcgtcgtgg caaaatgcat cggcataatc attacaaccg ctggtacatg
    36481 tagtccggct gcgatattcg gaataagact ggcaaatgac ataaacggcg cattgccaga
    36541 gcccatcaca atggcacaaa tggcaatgac tagcgccata ataatcatca cgccaatacc
    36601 gcccagacca gaatgctccg cacccctgat aaccgcatca acagtgccaa tcgtcgttaa
    36661 gcctttcgca aatatttcac ccgcgaccac cagcgttacc acgttgacaa actgcgtacc
    36721 catgccgtca aaaaacgcct gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa
    36781 gaactcaaca aacatcgtca ccgtcaaact tagtaacatc accaccacca gatgcagttc
    36841 tgacttcatc agcccgacgt gggcgaggaa cagcgagccg agcatcagga ttaacggcat
    36901 cacaggtaaa atggcgtaat agagcggcgg gacattatcg agagtttttt gctctgcctg
    36961 ttcgtgattg atatttttat cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat
    37021 cgccaccgag ataatgacgc aagaggcgac cggaagctgg tagtggaaga agtatgtggc
    37081 aattttcatt cccgcgacct gggctgcaaa aatggagttc gtttccagaa tcccccactc
    37141 aatggacatc gtggttgcga taaccgccac cgcagagagg cgacttactc ccagactcac
    37201 cagcgtcgga aataaggtga ccatcagcaa catacccaga ccggaggcgc tggtaataaa
    37261 ctgcgccatg atttggccga tgacgtaagt tgccgacaga ataatatacg gcgagcgaat
    37321 gagttttaac gggcggctta gcaagctcac catcgcgcga ctggctccta tgcgctccat
    37381 gtagcgggca taaccgccca ccgccataat cgacagcccc aatccggcaa tgcggttgga
    37441 cagcatgcgc aggatttcat tataaatgtc gacgatcaag tagccgctgc ttttggcggg
    37501 atcgagtaca ccaccgaacc ctaaccagac tccgcacatc atcaggaaga cacctgcggt
    37561 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg
    37621 ccgaac
//
LOCUS       DADLZM010000024        36607 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLZM010000024 DADLZM010000000
VERSION     DADLZM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.024"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="JNY73_001192"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="JNY73_001192"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY8047276.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /locus_tag="JNY73_001193"
     CDS             2619..3380
                     /locus_tag="JNY73_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8047277.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="JNY73_001194"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="JNY73_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY8047278.1"
                     /translation="MSSDADAHKVGLIPVILMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="JNY73_001195"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="JNY73_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY8047279.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="JNY73_001196"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="JNY73_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY8047280.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="JNY73_001197"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="JNY73_001197"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY8047281.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNY73_001198"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNY73_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY8047282.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNY73_001199"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNY73_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY8047283.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="JNY73_001200"
     CDS             complement(10310..11188)
                     /locus_tag="JNY73_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047284.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNY73_001201"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNY73_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY8047285.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="JNY73_001202"
     CDS             13815..14150
                     /locus_tag="JNY73_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY8047286.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="JNY73_001203"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="JNY73_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY8047287.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="JNY73_001204"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="JNY73_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY8047288.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="JNY73_001205"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="JNY73_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8047289.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="JNY73_001206"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="JNY73_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY8047290.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="JNY73_001207"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="JNY73_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY8047291.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVAEALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="JNY73_001208"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="JNY73_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY8047292.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="JNY73_001209"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="JNY73_001209"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY8047293.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="JNY73_001210"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="JNY73_001210"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY8047294.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLVHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="JNY73_001211"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="JNY73_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY8047295.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHVLEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="JNY73_001212"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="JNY73_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY8047296.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="JNY73_001213"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="JNY73_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY8047297.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="JNY73_001214"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="JNY73_001214"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY8047298.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNRIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="JNY73_001215"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="JNY73_001215"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY8047299.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="JNY73_001216"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="JNY73_001216"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY8047300.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="JNY73_001217"
                     /pseudo
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="JNY73_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
     gene            complement(25844..26173)
                     /locus_tag="JNY73_001218"
     CDS             complement(25844..26173)
                     /locus_tag="JNY73_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047301.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="JNY73_001219"
     CDS             complement(26242..26874)
                     /locus_tag="JNY73_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8047302.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRVAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="JNY73_001220"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="JNY73_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="JNY73_001221"
     CDS             29284..30804
                     /locus_tag="JNY73_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8047303.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="JNY73_001222"
     CDS             30801..31775
                     /locus_tag="JNY73_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY8047304.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="JNY73_001223"
     CDS             31806..32747
                     /locus_tag="JNY73_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8047305.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="JNY73_001224"
     CDS             32802..33485
                     /locus_tag="JNY73_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY8047306.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQGLMLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="JNY73_001225"
     CDS             33500..34360
                     /locus_tag="JNY73_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HAY8047307.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="JNY73_001226"
     CDS             34357..35358
                     /locus_tag="JNY73_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY8047308.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="JNY73_001227"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="JNY73_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36607
                     /locus_tag="JNY73_001228"
     CDS             36352..>36607
                     /locus_tag="JNY73_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047309.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         8526 a        10223 c         9724 g         8134 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gattacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt catcctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgccgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctgggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccgcccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgaagcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatggttc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg tgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg tgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttcggtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aatgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccgccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcgtattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tacaggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gtaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagc gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagcc gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccactcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctcg ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgctgcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaagtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacagg
    33001 ggctgatgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatc
//
LOCUS       DADLZM010000025        35744 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLZM010000025 DADLZM010000000
VERSION     DADLZM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.025"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..218)
                     /locus_tag="JNY73_001229"
     CDS             complement(<1..218)
                     /locus_tag="JNY73_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8047310.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRTLAEVY"
     gene            complement(399..1874)
                     /locus_tag="JNY73_001230"
     CDS             complement(399..1874)
                     /locus_tag="JNY73_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY8047311.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKCFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3300)
                     /locus_tag="JNY73_001231"
                     /pseudo
     CDS             complement(2021..3300)
                     /locus_tag="JNY73_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="JNY73_001232"
     CDS             complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="JNY73_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY8047312.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGITLAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5783..6529
                     /locus_tag="JNY73_001233"
     CDS             5783..6529
                     /locus_tag="JNY73_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY8047313.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6619..7380
                     /locus_tag="JNY73_001234"
     CDS             6619..7380
                     /locus_tag="JNY73_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY8047314.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7431..8315)
                     /locus_tag="JNY73_001235"
     CDS             complement(7431..8315)
                     /locus_tag="JNY73_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY8047315.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="JNY73_001236"
     CDS             complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="JNY73_001236"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY8047316.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9736..10149
                     /gene="msrB"
                     /locus_tag="JNY73_001237"
     CDS             9736..10149
                     /gene="msrB"
                     /locus_tag="JNY73_001237"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY8047317.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10191..10463
                     /locus_tag="JNY73_001238"
     CDS             10191..10463
                     /locus_tag="JNY73_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY8047318.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10833..11909
                     /locus_tag="JNY73_001239"
     CDS             10833..11909
                     /locus_tag="JNY73_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8047319.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11936..13304
                     /locus_tag="JNY73_001240"
                     /pseudo
     CDS             11936..13304
                     /locus_tag="JNY73_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13321..14366
                     /locus_tag="JNY73_001241"
                     /pseudo
     CDS             13321..14366
                     /locus_tag="JNY73_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14387..15223
                     /locus_tag="JNY73_001242"
     CDS             14387..15223
                     /locus_tag="JNY73_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY8047320.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15228..16176
                     /locus_tag="JNY73_001243"
                     /pseudo
     CDS             15228..16176
                     /locus_tag="JNY73_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16186..17166
                     /gene="ydjG"
                     /locus_tag="JNY73_001244"
     CDS             16186..17166
                     /gene="ydjG"
                     /locus_tag="JNY73_001244"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY8047321.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDPALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17303..18061
                     /locus_tag="JNY73_001245"
     CDS             17303..18061
                     /locus_tag="JNY73_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8047322.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18179..19537
                     /locus_tag="JNY73_001246"
     CDS             18179..19537
                     /locus_tag="JNY73_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8047323.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19630..20271)
                     /gene="pncA"
                     /locus_tag="JNY73_001247"
     CDS             complement(19630..20271)
                     /gene="pncA"
                     /locus_tag="JNY73_001247"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY8047324.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20282..21298)
                     /gene="ansA"
                     /locus_tag="JNY73_001248"
     CDS             complement(20282..21298)
                     /gene="ansA"
                     /locus_tag="JNY73_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY8047325.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(21465..23321)
                     /gene="sppA"
                     /locus_tag="JNY73_001249"
     CDS             complement(21465..23321)
                     /gene="sppA"
                     /locus_tag="JNY73_001249"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY8047326.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23482..24033
                     /locus_tag="JNY73_001250"
     CDS             23482..24033
                     /locus_tag="JNY73_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY8047327.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24150..25193
                     /gene="selD"
                     /locus_tag="JNY73_001251"
     CDS             24150..25193
                     /gene="selD"
                     /locus_tag="JNY73_001251"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY8047328.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSKMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25198..27159
                     /locus_tag="JNY73_001252"
     CDS             25198..27159
                     /locus_tag="JNY73_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY8047329.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIEQDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27286..28326
                     /locus_tag="JNY73_001253"
     CDS             27286..28326
                     /locus_tag="JNY73_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8047330.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMRLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28443..29786)
                     /gene="gdhA"
                     /locus_tag="JNY73_001254"
     CDS             complement(28443..29786)
                     /gene="gdhA"
                     /locus_tag="JNY73_001254"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY8047331.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARAITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30022..30294
                     /locus_tag="JNY73_001255"
     CDS             30022..30294
                     /locus_tag="JNY73_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY8047332.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30260..30667)
                     /gene="nudG"
                     /locus_tag="JNY73_001256"
     CDS             complement(30260..30667)
                     /gene="nudG"
                     /locus_tag="JNY73_001256"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY8047333.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30754..31374
                     /locus_tag="JNY73_001257"
     CDS             30754..31374
                     /locus_tag="JNY73_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY8047334.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(31383..32690)
                     /locus_tag="JNY73_001258"
                     /pseudo
     CDS             complement(31383..32690)
                     /locus_tag="JNY73_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
     gene            complement(32757..33410)
                     /locus_tag="JNY73_001259"
     CDS             complement(32757..33410)
                     /locus_tag="JNY73_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY8047335.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33410..34944)
                     /locus_tag="JNY73_001260"
                     /pseudo
     CDS             complement(33410..34944)
                     /locus_tag="JNY73_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34917..35744)
                     /locus_tag="JNY73_001261"
                     /pseudo
     CDS             complement(34917..35744)
                     /locus_tag="JNY73_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         9072 a         8462 c         9141 g         9069 t
ORIGIN      
        1 cagtacacct cagcgagagt gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 cttatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gagtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaac atttcattaa catgaaaaga
     1561 aaaatgatgg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgatttt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac aatgtctgat
     2161 gtgcacgacg ggtaatttcg atatagctgt aataacgaac aacaggtaat aattttttcg
     2221 ccaggatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg
     2281 cagcgtaaat attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt
     2341 tcagggcgct ggaaacaatg gtgccgcctg tttcctgcga gtagaaaaac tcatgttcat
     2401 cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc
     2461 tgctgaggaa cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat
     2521 ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc
     2581 gcttctcgta gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg tgttcaattt
     2641 tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt
     2701 cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg
     2761 ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg
     2821 ctggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt
     2881 gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat
     2941 ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggcct ggccctgacc
     3001 actgccggaa cctccgccgc caccctgggg acgttcaatt cggtcgttct gaacgaaatg
     3061 gtcattgccc ggatgcacgc ggtggcgcag accgccacgc ccctgatgaa acattggttc
     3121 gctaatatct tccgtgggaa tggatacgga ttcgctgctg tcgacgtcag tcaccgaacg
     3181 cttattaatg gcctcggaga tcgactgttt aatttgcgct ttataacggc gtaaaaaacg
     3241 ctggcgattc accatgcttt tgtttttgcc gttcagacgc cggtcaataa accaggtcat
     3301 atgccccccg tactacattt gccaactgta tgctacgcct gtgttgccgc ttctcaggcc
     3361 cacggttcgc tatcgttgta ggcccggtag catgtgtacc gggccagggt tgttaagacg
     3421 atttacgtac gcgcaaatac cattcgcaca gtaaacgcac ctgtttacgg gtgtagcctt
     3481 tttccatcat acggtcgaca aagtcgtcgt gtttcttctg ctcgtcggtt gacgttttgg
     3541 cgttaaacga gataaccggc aacagctcct cggtattgga gaacattttc ttctcgataa
     3601 ccgtgcgcag tttttcatag ctggtccagt tcggattgcg tccgctgtta ttcgctctgg
     3661 cgcgcagtac gaagttaaca atctcgttgc ggaaatcttt tggattactg atccccgccg
     3721 gtttctcgat tttctccagc tcggcgttaa gagactcgcg gtcaaacagc tgcccggtat
     3781 ccggatcgcg atactcctga tcctgaatcc agaaatccgc gtaggtaaca taacggtcga
     3841 aaatgttttg cccatattcg gaataggatt caaggtaggc cgtctggatc tctttgccga
     3901 taaactcggc atatttcggg atcagataac ctttcaggaa ctccagatag cgttctgcct
     3961 gctcttgcgg gaactgctcg cgctcgatct gctgttccag gacgtagaac agatggaccg
     4021 ggtttgccgc cacttctaca tgatcgaagt tgaacacgcg ggagaggatc ttaaacgcaa
     4081 aacgcgtcga cagaccgttc atcccttcat cgacaccggc gtagtcacga tattcctgat
     4141 acgacttggc tttcggatcg gtgtctttca gactttcgcc atcataaacc cgcatctttg
     4201 aataaatgct ggagttttct ggctctttca ggcgcgaaag aatggaaaaa cgtgacagtg
     4261 tttcgagcgt gccaggggcg catggggcgt gagtcaattc actgtgatta agcaatttct
     4321 cgtagatttt gatctcttcg gaaatgcgca agcaatacgg caccttcacg atgtaaacac
     4381 ggtcgaggaa ggcttcgttg tttttgttat tacggaaagt gacccattcg gactcgttcg
     4441 agtgggcgag agtaatcccg ttgaacggca gggcggagat accttccgtc ccgttgtagt
     4501 tatcttcctg agtggcggtt aacaagggat gcagcacttt tatcggtgct ttaaacatct
     4561 caacgaattc catgatcccc tgattggcgc ggcacagcgc accggaatag ccgtaggcgt
     4621 ccgggtcatt ctgcgcgtag tgttcgagtt tacgaatatc gactttccca accagcgcgg
     4681 agatgtcctg gttgttctca tcaccgggtt ccgttttggc gatagcaatt tgttgcagaa
     4741 ttgacggcca gaccttcact acccggaact tagtgatatc gccaccaaat tcatgcaggc
     4801 gttttgccgc ccatggcgac atgatggtgc cgagataacg gcgaggaatg ccatactctt
     4861 tttccagaat ctgagcatct tcctgcggat tgaaaagaca gaacggatga tcgttgaccg
     4921 ggctacgctc accgttggcg ctcaatacat aaatcggtac gagctgcatt aatgatttca
     4981 gtcgctcagc aagcgatgat ttacccccac ccacaggccc cagcagatac aggatttgtt
     5041 tcttctcttc cagcccctga gccgcgtgtt tcagataaga gacaatctgt tcaatcgcgt
     5101 cttccatgcc gtaaaactct tcaaatgccg gataacgtgc aatgacccgg ttagaaaaga
     5161 gtcgagaaag tctgggttcc tgggctgtat cgaccatgac aggctcaccg atagccatca
     5221 atagccgctc agccgcgttg gcataagcac tgcgatcttg ccgacaagtg gtaagaaact
     5281 cctgcagtgt gaactcttcg tccttggcag cttcatatcg ctggcgatag tgatcgaata
     5341 tattcatggt atgccgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata
     5401 gtagaggctc ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc
     5461 atgccatttt atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt
     5521 agatggcaaa tggaaaactt gcctgcacgg ctttcttaat ttcaatggca tatcaacaac
     5581 tcatccataa attttgcata attaatgtaa agaccaggct cgccagtaac gctaaatcca
     5641 tttggctgta agcgcggtgt catccgcggc aggaaaatta aacagttact ttaaaaaatg
     5701 aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca
     5761 tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat
     5821 cgcaacgtct gcaggcgttg cgcacgctga aggtaaattt tccctgggcg caggcgtagg
     5881 tgtcgttgag cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa
     5941 ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga
     6001 cgcaacggat aaactttcaa ttaccgctta ctggtcgccg ctttacttca aagcgaaaga
     6061 cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct
     6121 gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg gcgataccct
     6181 ggataacagc aacggcattg tctgggatat ggcttggttg tatcgttaca ccaacggtgg
     6241 cctgaccgtg actccgggta ttggtgtgca gtggaacagc gaaaaccaga acgaatacta
     6301 ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtgggtata acccgaatga
     6361 cagctggagc ccttaccttg agctgagcgc cagctacaac ttcctcggcg actggagtgt
     6421 ttacggtacc gcgcgctaca cccgtctgtc tgatgaagtt actgacagcc cgatggtgga
     6481 taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt
     6541 tccagggcat tttgtgcata aaaatagggc gtcacgccct gttttgcatt acggtggtgc
     6601 aatacctggg aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg
     6661 ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa
     6721 gttgctgcac tacgcgcagg cattgaactc ggtttaaccc tcattgatac cgccgaaatg
     6781 tatgccgatg gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgagagaag
     6841 gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca
     6901 tgcgaagcca gtttacgccg tctcaatact gattatctcg atctctactt attacactgg
     6961 tctggcagtt tcgcttttga agagactgtt gctgcgatgg aaaaattgat cgcccaggga
     7021 aaaatccgtc gctggggcgt ttctaacctt ggttatgccg atatgcagga actctggcag
     7081 ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg
     7141 attgagtacg atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt
     7201 ccattagtcc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa
     7261 attgcacatg tccaacaaaa tgcagctgcg cttgaggtcg aactttcgtc agcggaatta
     7321 gctatgctgg ataaggcata tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga
     7381 tgttgatacg tgccggatgc atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttg
     7441 gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt tggtgacttt ttgcgtttct
     7501 gaagcgtaag ccgtttctac acaaacaaag gtcttgtagc catcgtccgg catgtcgccc
     7561 atgctaactg aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg
     7621 cctacggcga taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgcggattc
     7681 agatacacgc gatcggtacg gtcagggaag gtctgaatac cgtcggtcag tacatcttct
     7741 ttcgcgtcat tcactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg
     7801 atatcaccca cgttaaagta ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat
     7861 tcaagatcga tttcacaggt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc
     7921 ggccagaatt ttttcgtctc ttcgctttgt gtcaattcaa aagtcagcgc tacgccatca
     7981 gcatcttcat gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc
     8041 agaccttgtt gtgccgccgg accaaaccac ggccagcaaa ccggtacgct accgcgaata
     8101 gcgacgccat ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca
     8161 ggtttccacg agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga
     8221 tcgaccacaa tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt
     8281 tcgatgaccg gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta
     8341 tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt
     8401 atttggagat gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt
     8461 accaagatac cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca
     8521 cggaagtgca aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca
     8581 gaacgccttt catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag
     8641 ttgcagcttt ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc
     8701 ggaacgccat accagtcagt ttgccattca gttctggcag tactttacct acagctttag
     8761 cagcaccggt agaggacggg atgatgttct gggaagcgcc gcggccgccg cgccagtctt
     8821 tgtgagacgg gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca
     8881 gaccttcgat gatgccgaag ttatcgttga taactttagc cagcggagcc aggcagttgg
     8941 tggtgcagga agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt
     9001 taacgaacat cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag
     9061 cggtgatgtg tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa
     9121 cgtcaacacc cacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga
     9181 tttttttacc gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc
     9241 cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga
     9301 ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac
     9361 ggccaaaacc gttgatacct actttgatag tcatatattc caccagctat ttgttagtga
     9421 ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa
     9481 tcattgcgac aaatcaatcc tgtgcctaag cattacgcgg ctgactcgcc tcactcttcc
     9541 tttgggcttg agaccacatg gggtcggcgc cccgaatttt aaagggcaat tacgataaaa
     9601 atgtgatttt catcacgatt taggtgcagc catttcgtcg agatcaagtt taggacaaaa
     9661 ttttgccagt tggtgttaat gttttgttag aatcagtcac gtaatgtgag cacgattaaa
     9721 gtgagatgtg agtaaatggc gaataaacct tcggcagaag aactgaaaaa aaatttgtcc
     9781 gagatgcagt tttacgtgac gcagaatcat gggacagaac cgccatttac gggtcgttta
     9841 ctgcataaca agcgtgacgg cgtatatcac tgtttgatct gcgatgcccc gctgtttcat
     9901 tccgaaacca agtatgattc cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa
     9961 gaatccattc gttatatcaa agacttgtca catggaatgc agcgcataga aattcgttgc
    10021 ggtaactgtg atgcccatct ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa
    10081 cgttattgtg ttaactctgc ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc
    10141 aacggttgaa aaaacgattc agcaacttat tccacaggag cggaaaaatt atgaatcttg
    10201 atgacattat caacagcatg acgcctgagg tataccagcg tttgtcgacc gccgttgaac
    10261 tggggaaatg gcctgatggc gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac
    10321 tggtgatgct gtggcaagcc cgccataata ccgaagcaca gcatatgaca attgacacaa
    10381 atggtcagat ggtcatgaag agcaaacagc agttaaaaga agattttggt atctcagcaa
    10441 agcctattgc catgtttaag taagtggttc atcccctttt tgccgcggct atgaagggga
    10501 tagccaacaa tgaccccacg actcgtctca attatctcga cccgacctgg ctaagtccca
    10561 gtccgatctc ttgcagtcgc gttcacgcct gaatgacttc tcctgaactt aagtctcaga
    10621 cctatttggc cggtaatccc tctcgaattt gctctttttg cgtcccgcct tccaccaacc
    10681 tgtaacagac ccagccattt tctgcgtaat tgtgatctaa gttgtaaaat gcaggtcatt
    10741 atctgtgttg tgcagattta atgttgacct caaaaatcat gtgggtaagt atgatggcca
    10801 tctaagcaaa acaaaacaca gaggatgacg caatgaaagc actggctcgg tttggcaagg
    10861 cctttggcgg ctacaagatg attcatgtcc cacaacccat ttgtggcccg gaagacgtag
    10921 tgattgaaat taaagccgcg gcaatctgcg gcgcagacat gaagcactac aatgtcgata
    10981 gcggttctga tgagtttaac tctatccgcg gccatgagtt cgcaggttgt attgcgcagg
    11041 ttggtgaaaa agtcaaagac tggaaagtgg ggcaacgcgt cgtgtcggat aacagcggtc
    11101 acgtttgcgg cgtttgtccg gcctgtgaac agggtgattt tctgtgttgt acagaaaaag
    11161 taaaccttgg tctggataac aatacctggg gcggtggttt ttccaaatat tgtctggttc
    11221 ctggtgaaat tctcaaaatt catcgtcatg cgttgtggga aatccctgat ggtgttgatt
    11281 atgaggacgc agccgtactt gaccctatct gtaatgccta taaatccatc gcgcagcaat
    11341 cgaaattcct tcctggtcag gatgttgtcg tcattggcac tggcccactc gggctgtttt
    11401 ccgtacaaat ggcgcggatt atgggggcgg taaatatcgt cgtcgttggt ctgcaagaag
    11461 atgtggcggt ccgcttcccg gttgcaaaag aactgggggc gacggcagta gtaaatggtt
    11521 ctaccgaaga tgtggtggcg cgctgccagc aaatttgtgg caaagataat ctgggactgg
    11581 tgattgaatg ctccggtgcc aatatcgcac tgaaacaagc catcgaaatg ctccgcccga
    11641 atggggaagt ggtacgcgtt ggaatgggct tcaaacctct tgatttctcg attaatgaca
    11701 ttaccgcctg gaataaaagc atcattgggc atatggccta tgactccacc tcatggcgta
    11761 acgctatccg gctattagcc agcggcgcta tcaaagtcaa accgatgatc acgcatcgta
    11821 tcggcctgtc gcaatggcgc gaagggtttg atgcgatggt cgataaaacc gcaatcaaag
    11881 tgatcatgac ttacgacttt gatgaataac tactctcctg aaggaacgtt atcgcatgga
    11941 acagataaca aaaccgcatt gtggtgtacg gctggatcgc ttaccggatt gccgctggca
    12001 ttcgtcaatg tttgccatcg tcgcgtttgg cttgcttgtc tgctggagta atgccgttgg
    12061 tggcttgatc ctcgcgcagc tgaaagcgtt gggctggaca gataattcca ccacagccac
    12121 attctcagca atcacgaccg ccggaatgtt tctcggtgcg ctggtgggcg gcatcattgg
    12181 cgacaaaacc ggtcgcagaa atgcgttcat cctctatgag gccattcata ttgcctcgat
    12241 ggttgtcggt gctttctcac cgaatatgga ttttcttatt gcctgccgtt ttgtgatgga
    12301 cgttgggctg ggagctttac tggttacact gtttgctggt ttcaccgaat atatgcccgg
    12361 tagaaatcgc ggaacgtggt ccagtcgggt ttcctttatt ggcaactggt catatccgct
    12421 ctgctcactg attgcgatgg gactcacgcc gctgattagt gcagagtgga actggcgggt
    12481 acaactgctt attcctgcaa tattgtcgct tatcgctacg gcgctggcct ggcgctactt
    12541 tcctgaatcc ccgcgctggc tggaatcgtg cggacggtat caggaagccg agaaagtcat
    12601 gcggagtata gaagaaggcg tcatacgcca gacgggtaaa cctttgccgc ctgtggttat
    12661 tgctgatgac ggtaaagcgc cacaagcggt gccgtattca gccttactga caggagtatt
    12721 actgaaacgc gtgatattag gctcttgtgt gctgattacc atgaacgttg tgcagtacac
    12781 actaattaac tggttgccaa caatattcat gacccagggg attaatttaa aagactcgat
    12841 tgttttaaat accatgagta tgtttggcgc gccatttggt atttttattg ccatgctggt
    12901 gatggataaa attccgcgta aaacaatggg tgtggggcta ttaatcctga ttgcggtgct
    12961 cggatatatc taccagtatg ttgctcataa cgctgattgg tttcttcctg attactttcg
    13021 tctatatgta cgtttgctat gcctcggcag tgtatgtccc tgaaatctgg ccgacagagg
    13081 ccaaactccg tggctccggt ctggcgaatg cggtagggcg aatcagtggt attgccgcac
    13141 cttatgccgt tgcagtgctg ctcagtagtt atggtgtaac gggagtcttt attcttctgg
    13201 gggctgtttc aattattgtc gcaattgcta tcgccaccat tggaattgaa accaaaggtg
    13261 tctccgttga aagtttaagt attgatgcag tagccaataa ataatacggg tggattcaaa
    13321 atgaaaaatt caaaagcaat attgcaggtg ccgggcacaa tgaaaattat ttcagcagaa
    13381 ataccagtgc ctaaagaaga tgaagttttg attaaagtag aatatgtcgg tatttgtggt
    13441 tcagatgtac atggttttga atcaggcccg tttattccgc ctaaagaccc aaatcaagaa
    13501 attggcctgg gtcatgaatg cgccgggacg gttgtggctg tgggaagccg cgtgcgcaaa
    13561 tttaaaccgg gggatcgggt aaatatcgaa cctggcgttc cttgcggtca ctgtcgttac
    13621 tgtctggaag gcaaatataa catctgcccg gacgttgatt ttatggcgac acaacccaac
    13681 taccgcggcg cattaacgca ctatctgtgt catccggaga gctttactta caaactgccc
    13741 gacaatatgg acacgatgga aggggcgctg gtggagcctg ccgcagtcgg gatgcatgcc
    13801 gcgatgctgg cagatgttaa accgggtaag aagataatta ttctgggagc aggttgtatt
    13861 ggtttgatga cgttgcaagc gtgcaaatgc ctgggcgcaa cggaaattgc cgtcgttgat
    13921 gtactggaaa aacgtctggc aatgggggaa cagcttggtg cgacagtggt tattaatggc
    13981 gcaaaagagg acactattgc acgctgtcag caatttaccg aagacatggg cgcagatatt
    14041 gttttcgaaa cagcgggttc tgcggtcacc gttaaacagg cacctttaat ctggtaatgc
    14101 gcggcggtaa aattatgatt gttggtactg tacccggcga ttcggcaatc aatttcctca
    14161 aaatcaatcg cgaagtcact atccagacgg tattccgcta tgccaatcgt tatccggtca
    14221 cgattgaagc tatttcttca gggcgattcg atgtgaaatc gatggtgacg catatttacg
    14281 attatcggga tgtacaacag gcatttgaag agtcagttaa caacaaacgc gacattatta
    14341 aaggcgttat taaaattagc gattaattaa tacgggagaa cagaacatgc tcgcagatat
    14401 cagatattgg gaaaacgatg ccacaaataa acattatgca attgcccatt ttaacgtatg
    14461 gaatgcagaa atgttgatgg gcgttaaaga cgcagccgaa gaagcgaaat ccccggttat
    14521 tatttctttt ggtacaggtt ttgtcggtaa cacctcattt gaagatttct ctcacatgat
    14581 ggtatcaatg gcacaaaaag caacggtgcc ggtaataact cattgggatc atggtcggag
    14641 tatggagatt attcataacg cctggactca tggtatgaat tcattaatgc gtgatgcttc
    14701 tgcatttgat ttcgaagaaa atattcgttt aaccaaagag gctgttgatt tctttcatcc
    14761 gctgggtatt ccggtagagg cggaattagg gcatgtcggt aatgaaaccg tttatgagga
    14821 agcgctggcg ggctatcact ataccgatcc tgaccaggcg gctgaatttg ttgaacgtac
    14881 gggctgtgac tcattggctg tcgccatcgg caaccagcat ggggtttata cgtcagagcc
    14941 gcaattgaac tttgaggtcg tcaaacgcgt acgcgatgct gtttctgttc cgctggtttt
    15001 gcatggcgca tcggggatca gtgatgccga cattaaaact gcaatttcgt tgggtattgc
    15061 gaaaatcaac attcatacgg agctctgtca ggccgcaatg gtcgcagtga aagaaaacca
    15121 ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa gctgtaaaag aacgggcatt
    15181 ggaaaaaatt gaactgttcg gttctgatgg caaagcggaa taatgatatg gataatctcg
    15241 acgttatttg tataggtgcc gctattgttg atattccatt acaaccggtc agtaaaaata
    15301 tctttgatgt agattcttat cctcttgaaa gaatcgcaat gagcaccggg gggcgatgca
    15361 ataaatgaag caacaattat ttctcgtctg gggcatcgta ccgcattaat gagccgcatt
    15421 ggtaaagatg cctcagggca atttattctc ggccattgtc gcaaagagaa tattgatatt
    15481 caaagcctga aacaggacgt cagcatagat acatctatta acgttggact ggtgacggag
    15541 gatggcgagc ggacgtttgt cacgaaccgt aacggcagtt tgtggaaatt aaatattgac
    15601 gatgttgatt ttgcgcggtt tcctcaggcg aaattattat cactggccag tattttcaac
    15661 agtcctctat tggatggtaa agcgctaaca gaaattttta cgcaagcgaa agcccggcag
    15721 atgatcattt gtgccgatat gatcaaaccg cggttgaatg aaacgctgga tgatatttgc
    15781 gaagcattga gttatgtcga ttatctgttt ccaaattttg ccgaggcaaa attactcaca
    15841 gggaaagaga cactggatga aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg
    15901 gtgattaaaa cgggtaaaga cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg
    15961 ccggcggtcg caggaataac cgccatcgac accattggcg cgggcgataa ctttgcttca
    16021 ggttttattg cggcactgtt agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat
    16081 gcaacggcgg ctatctcggt tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag
    16141 ctggtcgaac aattgctgga agaatacgaa ggataatgaa ggcaaatgaa aaagatacct
    16201 ttaggcacaa cggatataac gctttcgcga atggggttgg ggacatgggc cattggcggc
    16261 ggtcctgcat ggaatggcga tctcgatcgg caaatatgta ttgatacgat tcttgaagcc
    16321 catcgttgtg gcattaatct gattgatcct gcgctaggat ataactttgg caatagtgaa
    16381 gttatcgtcg gtcaggcgtt aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa
    16441 tgcggcattg tctgggaacg aaaaggaagt ttattcaaca aagttggcga tcggcagttg
    16501 tataaaaacc tttccccgga atctatccgc gaagaggtag aagccagctt gcaacgtctg
    16561 ggtattgatt acatcgatat ctatatgacg cactggcagt cggtgccgcc attttttacg
    16621 ccgatagctg aaactgtcgc agtgcttaat gagttaaagg ctgaagggaa aattcgcgct
    16681 ataggcgctg ctaacgtcga tgctgaccat atccgcgagt atctgcaata tggtgaactg
    16741 gatattattc aggcgaaata cagtattctc gaccgggcaa tggaaaacga actgctgccg
    16801 ctatgtcgtg ataatggcat tgtggttcag gtttattccc cgctagagca gggattgttg
    16861 accggcacca tcactcgtga ttacgttccg ggcggcgctc gggcaaataa agtctggttc
    16921 cagcgtgaaa acatgctgaa agtgattgat atgcttgaac agtggcagcc actttgtgct
    16981 cgttatcagt gcacaattcc cactctggca ctggcgtgga tattaaaaca gagtgattta
    17041 atctccattc ttagtggggc tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg
    17101 aatatcaact tatcggatgc agacgcaaca ttgatgaggg aaatggcaga ggccctggag
    17161 cgttaaatac tcaacacgat tcacctctgt ttctgtgaaa actgtgatga aaatctgtga
    17221 ttcaaatagg ttattttgaa agtaaacatc ggttatgcga taatcgcgct aatgtgatgt
    17281 aaaaagatac aggggtgtaa tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat
    17341 ggttgccaac gataaaaagg tgacagtctc aaatttgagt gggatttttc aggtaaccga
    17401 agaaaccatt cgccgcgatc ttgagaagct ggaagatgaa ggctttttga ccagaaccta
    17461 tggtggtgct gttttaaata cagcggtgtt gacggagaat atccattttt ataagcgcgc
    17521 ttcatcgttt tatgaagaga agcagcttat tgcacgcaag gcactaccct ttatcgacaa
    17581 taaaaccacc atggcagccg attccagtag taccgtaatg gaattgctca aattgttaca
    17641 ggaccgtagt gacctgacgt tgctaaccaa ctcggcggaa gcgattcatg ttctggctca
    17701 gtcagaaatt aaagtcgttt caacgggtgg ggaactaaac aaaaatacac tttccctgca
    17761 aggaagaatt actaaagaga tcatcagccg ctaccatgtc gatatcatgg taatgagttg
    17821 taaaggtctt gatattaaca gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa
    17881 aaagacaatg atccgtcagg cgacagaagt tgcgttattg gttgatcact ctaagtttga
    17941 tcgtaaagct tttgtccagt tagctgactt tagtcatatt aattatataa taactgataa
    18001 atcaccgggt gcagagtgga tagcattttg caaagacaat aatatccagc tggtgtggta
    18061 atttaagcac acgttgtcaa tattatttgt tcctgcgcct ggaaataaaa tgatgataaa
    18121 gatgcggcaa gtgaatttgc cgtaattgct accttttata gcaaatccga ggcacccaat
    18181 ggaacaatat gatcaaattg gcgcaagact ggaccgcttg cctttggccc ggtttcatta
    18241 tcgtatattt ggtataataa gctttagtct gttattaacg gggtttttga gttactcagg
    18301 taatgtcgtc ttagcaaagc tggtaagcaa tggatggtca aataatttcc tcaatgccgc
    18361 ctttacctcg gcattaatgt ttggttattt catcggctca cttactggtg ggtttattgg
    18421 tgactacttt gggcggcgca gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc
    18481 aacaggggcc gcttttgtcc ctgatatgta ctggctcatt ttcttgcgct tcctgatggg
    18541 aacaggaatg ggggcgctga ttatggttgg ctatgcctca tttacggagt ttatccccgc
    18601 gacggtgcgt ggaaaatggt ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct
    18661 gtctgcggcg ataggcgtgg tggttatcgc tttttttagt tggcgaataa tgtttcttct
    18721 gggtggtatt ggcatactgt tagcctggtt tctctcaggt aaatacttta tcgagtcgcc
    18781 acgatggctg gcagggaaag ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga
    18841 gcagcaaatt gaaagggaga agagtattcg tttaccccag cttactttga accagagcaa
    18901 cagcaaggtt aaggtaatca agggtacctt caggctcctg tttaaagggg aaatgttacg
    18961 acgtacatta gttgcgataa ctgttttaat tgcaatgaac atttcgcttt ataccattac
    19021 cgtatggata ccgaccatat ttgttaactc cggcattgat gtcgataaat caatattaat
    19081 gaccgctgtt attatgattg gtgctccggt aggaatattt attgcggcat taattattga
    19141 tcattttcct cgtcgattat ttggctccgc cttacttatt attattgccg tgttaggcta
    19201 tatctattca agtcagacta cagagtgggc gattttaatc tatggtctgg tgatgatctt
    19261 ctttttatac atgtatgttt gcttcgcgtc ggcggtttat atcccggagc tttggccaac
    19321 gcatttacgc ctgcgcggtt cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt
    19381 cacgccctat ggcgttgcgg cattattaac acattatggg tcgatcacgg tgtttatggt
    19441 gcttggtgtc atgttagtgc tctgtgcgct ggttctctcc atttttggca tcgaaacgcg
    19501 gaaggtgtcg ctggaagaga tttctgaggt gaattaaatc tttcgttgat ggcatcaatt
    19561 gccagatgta ccactgacgt gttttatttg gcctacaatg ccagtacaga attgtaggcc
    19621 agcgtaaaat tacccctgcg tctcttccca gtctgccagc gtatatagcg ttgccccagc
    19681 tgctgacatc tccataaacg cgtgtgcact gtcctggggc tggatattca cgccacgaca
    19741 gccatcggta atcacgttta ccttataacc taactgtaac gcgtccagca cggtaaactt
    19801 cacgcaatag tcagtagcca ggcccataac gatcaattca tcgatttcat gatcgcgcaa
    19861 ccagtcatca agcgcggttt tctgccgacg tccgttatca aaaaaggcac tgtaactgtc
    19921 aactaaagga ttttcgcctt tatggaacac cgctgcgatc tctttttgat tcagtaacgg
    19981 atgtaattgt gcgccttcac tgttctgcac acagtgttca ggccagaaag tttgccgcag
    20041 gccgtcgagt tggcctggcg tataaggctc tacgccgtgc tggctggcaa agctgccgtg
    20101 attcgccgga tgccagtcct ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc
    20161 aataaggcgg ttagcaacct ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc
    20221 agcacagaaa tcattttgta aatcgaccag taacagggcg cgatggggca ttgcagtctc
    20281 cttaatcatc cggcgtcagt tcgccgcgca ggttttggct catggccttg cgaatggttt
    20341 cagtatccag ttcctggctc agcaggtaat gcagtttggt tagcgtggct tctacagtca
    20401 tatctgcacc gccaataacg ccggcatggg cgaggacgtt accggtggca taaccactca
    20461 tattcacttt accggacata cattgtgtca ggttgaccac cacaataccg cgatcgctgg
    20521 cttcttgtaa ttcctgcagg aaggctttgt tttgtggcgc attacccacg ccataggagc
    20581 gcagaatcaa tgctttcacc ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc
    20641 ctggataaat cgtcactacg ccaattggtt gtggcgtgat tggatgaacg attaattccc
    20701 cttcaccgtg cggggcgggt ggcgtattca aacgacgaat atggatacca gcttccagta
    20761 acggaggaag gtttggagag gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc
    20821 ggttgccgcg atacaatcgg ttattgaaaa agagcgttac ttcgttgatc ggataattcg
    20881 ccgcaacgta caacgcattc agcagattaa tttgtccgtc agagcgtaac tcagccagcg
    20941 ggatttgtga ccctgtcaca atgaccggtt taccgagatt ctcgagcatg aacgacagcg
    21001 cagaggcggt atacgccatc gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt
    21061 catagtgcgc tttaatatct tcagcaatat gctgccagtc ttccggcgtc atatcagaag
    21121 aatccatcaa cggcgtatat tcatgaatgg tgaaatctgg catctccagg cgatggaatt
    21181 ccggcatcag cgccagttgg cgttgtagat gacctgacac cggaatataa ccctgctcgg
    21241 aacgctgcat cccgatggtc ccgcccgtgt aggcgacgta aattgatttc ttttgcatga
    21301 tattgatatg aatgatatcg aaagagcaaa agtatatacg tgaggcagga ggagcgcacc
    21361 tgatactatg cggataagga aatatggggc gagacagcgg gaagaggatt cgtcgaggta
    21421 aaaaataatg tggccgacga tctgtcggcc actcagtaca agacttaacg cacgttggcg
    21481 caggtcaggc aaaacgcata acggttttgt gggtcgttaa acgcggccag cttgtcgctt
    21541 tcacttttaa cggtagaggc taccgaggca agcggtgcag gtaacatggc ctggaacgca
    21601 tctggcaaca ttgcccggac agaaccagac atgttgtcca tcactttgtc gaagaaggtc
    21661 ggttcatcaa cgtagtattc cagatgccac tgtttcactt ttgcgagctc tgctgctttg
    21721 gcaaccgcat catcgaaatc cccgagacta tcgaccagcc cgttagcttt tgcatcctga
    21781 ccggtccaga cgtggccctg ggcgatttta tcaatttgct ccggcgtcga atgacgcgca
    21841 tcagcaacca gcgtgataaa gcgtttatag ccattctcaa tgcttaattg catcatctgc
    21901 tgcgcttccg gcggcagtgc cctggtgata gaaacatccg ccagcggtga agttgagaca
    21961 ccatcagtat gaacaccaat cgaatccaga ctattttcta cggtggtgat cacgccgaag
    22021 ataccgatag aaccggtcag ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa
    22081 atccagtaac caccagatgc cgccatgccg cccatcgata caaccacaga cttacccgct
    22141 gcccgggctg ctgccagttc agcgcgaatc acttcagacg cggtaacgct gccgcctggg
    22201 ctattaacac gcaggacaat cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt
    22261 gccgcagtgg tatcaccgcc aacattcccc tgagtttcct cgccatccat aattgcgcca
    22321 ttagcgaaga cgacaccgat gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg
    22381 taataactga tggcgcgata atttttatca gtcttactcc agccgaactc tttggtcaga
    22441 gctttttcga tttcggcact cgatgccagt gcatcgacca gcttgttttc cagtgcatat
    22501 ttcgcggtat cgccaccggt tttggttaaa ccctcaagca acccttgcgc gccagggaat
    22561 acctgctgag cagggatctg ccggttagcg gcaacagtat tcagatagtt ttgccacagc
    22621 tcaccaatcc agcggctgtc agcttcgcgg gctgccggtg acatatcatc acgaataaac
    22681 ggttcaacgg cagatttata cgtacccacg cggaacacat gggtggaaac tttcagctta
    22741 tccagcaacg atttgtagta cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct
    22801 tgcggtgaca gccaaatttt attggcgaaa ctggcgagat aatattgccc ctggctgtag
    22861 ttctcgccaa cggcataaac cggtttcccg ctgtcacgaa actctttcag ggctttgccg
    22921 atgtactgca tagacggctg gtcgccgcct gcgaagtttt tcagatccat cacgataccg
    22981 gtgatattgc ggtcgtcctt cgcctggcga atggtgttga cgatatcaaa cagtgagttt
    23041 tcctgcagac gatcggaact ggcaccaagc agctggcggc ttaatttact aaaccgctga
    23101 gaactgtctg gtttatcgac gatcacacca gaaatgtcca gcagcagtgc gccacgactg
    23161 gccgtttctt tcgaatcacc accactgacc tgcatccaga tccccacacc aaccagtacg
    23221 aggaaaataa agaacaggtt aagtaccatt tcacggacga aattcagcag acgccacgtc
    23281 catttaaaaa atccggcaat aaatcgccaa agggttcgca tgtattctcc caacttaagg
    23341 tcacacctgt cacaggcgca atagatgtgg ttatcgtaat gacccaaccg gcacttgtca
    23401 gcaggaattg cggactgtgc tgtaacaaaa tccactctcg tgttaatttt gtgaataaat
    23461 atcacgacag gagttaatca aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc
    23521 tcccgcttgg ctgaacccgc gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt
    23581 atgcgtgcgc cggaccataa gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa
    23641 gggtgcgagc gtttcagcgc cgtactggaa cagggggcga ttgctgccgg tagtgatgat
    23701 aaagctatcg acaaagcccg taatgcgccg ttccgcgcac cgctcatcat cacggtggtg
    23761 gcgaaatgca aagaaaatca taaagtcccg cgatgggaac aggaaatgtc tgccggatgc
    23821 gcggtcatgg cgatgcaaat ggcagccgtt gcccagggat ttggcggcat ctggcgcagt
    23881 ggcgcattaa ctgaaagtcc ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa
    23941 attgtcggtt ttctctacct cggcacgccg cagttgaaag catctacgtc gattaacgtc
    24001 ccggacccga cgccgtttgt aacttatttc tgataattgt cgcgaaactg tctggattgt
    24061 gagcaaaggc gcaggaattc agacactctc acttatcact tcacggaatg agggttacca
    24121 tacccggatt gcattgacag gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat
    24181 acagccacgg agctggttgc ggctgtaaaa tttccccaaa agtgttggaa accatcctgc
    24241 acagtgagca ggcgaagttt gttgatccga atttgcttgt gggtaatgaa acccgcgacg
    24301 atgcggcggt gtacgatctg ggcaatggca ccagcgttat cagtaccacc gacttcttta
    24361 tgccgatcgt tgataatcct ttcgattttg gccgcattgc ggcgactaac gccatcagcg
    24421 atatcttcgc gatggggggc aaaccgatta tggcgattgc gatcctcggc tggccgatta
    24481 acaaactttc cccggaaatt gcccgcgaag taaccgaagg tggacgctat gcatgccgtc
    24541 aggcgggtat tgcgctggct ggcggtcact ccatcgatgc gccggagccg atttttggtc
    24601 tggcggtaac ggggatcgta ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg
    24661 gatgcaaact gttcctgacg aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa
    24721 aatcactgtt gaaaccagaa catcagggac tggcgacgga agtgatgtgc cggatgaaca
    24781 tcgcaggcgc gtcctttgcc aacatcgaag gcgtaaaagc gatgaccgac gttacgggct
    24841 ttggtctgct gggccacttg agcaaaatgt gtcagggggc tggtgtgcag gcacgcgtcg
    24901 actatgaagc gatcccgaaa ctccccggtg ttgaagagta cattaagttg ggcgcagtac
    24961 ctggcggcac tgaacgtaac tttgccagct acggtcatct gatgggtgaa atgccgcgtg
    25021 aagtgcgcga tctgctgtgt gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc
    25081 cggaagcaga aaatgaggtt aaaactacag ccgccgagtt tggcattgaa ctgacggcga
    25141 ttggcgaact ggtgccagcg cgcggcggtc gtgccatggt tgagatccgt taattcaatg
    25201 cggttgttta ttgccgaaaa accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa
    25261 ccgcaccgga aaggcgatgg ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt
    25321 atcggtcacc tgcttgagca ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg
    25381 aatcttgcgg atttgccgat tgtcccggaa aagtggcaat tacagccacg accctccgtg
    25441 accaaacaac ttaacgtcat caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc
    25501 ggggacccgg atcgtgaagg gcaattgctg gtggatgaag tgctggacta tctgcaactg
    25561 gcaccggaaa agcgccagca ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg
    25621 gttgagcggg cgatcgaccg tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct
    25681 gcgctggcgc gagcgcgtgc cgactggctg tacggcatca atatgacccg tgcgtatacc
    25741 attctcggtc gcaatgccgg ttatcagggc gtactttccg tgggacgcgt gcagacaccc
    25801 gtgctcgggc tggtggtgcg ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc
    25861 tttgaagtca aagcacatat cgtgacacct gccgatgagc ggtttaccgc tatctggcaa
    25921 ccgagcgaag cgtgtgaacc gtaccaggat gaagaagggc gcttgttaca tcgtccactg
    25981 gcggagcatg tggttaaccg cattagtggt caaccggcta ttgtcaccag ctataacgat
    26041 aaacgggaat cagaatccgc gccgctgccg ttttcgcttt cggcgttgca gattgaagcg
    26101 gcaaaacgct tcggtctgag cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa
    26161 acgcacaagc taatcactta tccgcgttcc gattgtcgct atttgccaga agaacatttt
    26221 gccggacgcc acgcggtgat gaatgccatc agcgttcatg caccggatct gttgccgcag
    26281 ccagtggtag atccagatat acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac
    26341 cacgccatca tcccgaccgc acggagttct gcgatcaacc tgacggagaa cgaagcgaag
    26401 gtctataacc tgattgcccg tcaatacctg atgcagttct gcccggatgc ggtgttccgc
    26461 aagtgtgtta tcgaacagga cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt
    26521 gctgaagcag gctggcgcac gctgttaggc agcaaagagc gcgatgaaga aaacgacggc
    26581 acgccattgc ctgtggtggc gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta
    26641 gagcggcaaa cccagccgcc gcgccatttt accgatgcaa cactgctttc ggcgatgacc
    26701 gggatcgcgc gctttgtgca ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt
    26761 ctggggacag aagcaacccg tgccgggatt attgaactgc tgttcaagcg tggttttctg
    26821 accaaaaaag ggcgctatat ccactccacc gacgccggaa aagcgttatt ccattcgctg
    26881 ccagaaatgg cgacgcgacc ggacatgacc gcgcactggg aatcggtgct gacgcaaatc
    26941 agcgaaaagc agtgtcgcta tcaggacttt atgcagccgc tggtggggac gctatatcag
    27001 cttattgatc aagccaaacg tacgccggtg cggcagtttc gcggcattgt ggctccgggc
    27061 agtggtggca gtgctgataa gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt
    27121 ccgccagcag atgaagccgg aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta
    27181 ttcgagtcat attctgaaat atccagcgga tcaagaaaat tcgttggata tttttttgca
    27241 tggataaaat tatcgcctct aaagtatgta ataacaggga atgtgatgaa aaaagtttta
    27301 ttacaaaacc atcctgggag cgagaagtat tcttttaatg gctggggaat atttaatagt
    27361 aattttgaac gggtgattaa agaaaataag gccatgctgc tttgtaagtg ggggttttat
    27421 ttaacatgtg ttgtcgctgt aatgtttgta ttcgcagcga taacatccaa cggtttgaat
    27481 gaaagaggcc tgattaccgc gggatgctct tttctttatc tattaattat gatgaggctt
    27541 attgttcggg ccggttttaa agcaaaaaaa gaacaactac attattatca ggctaaaggt
    27601 attgagccgc tcagtatcga gaagttacag gcgctacaat tgatcgcacc ttatcgattc
    27661 tatcataagc aatggtctga aacgctagag ttctggccgc gaaagcctga acctggcaaa
    27721 gataccttcc aatatcatgt gcttcctttt gactcgatcg atatcatcag taaaagacgc
    27781 gagtctttag agaatcaatg gggtatcgaa gatagcgaaa gttattgtgc cttaatggag
    27841 cattttcttt ctggcgacca tggagccaat acctttaaag caaacatgga ggaagcccca
    27901 gagcaggtta tcgccgtgtt gaataaattt gctgtttttc cctcagacta tatctctgat
    27961 tgcgctaata atcgctccgg taaatcctcg gcgaagctaa tatgggcggc ggaattatca
    28021 tggatgatct cgatatcaag cacagctttt caaaacggga caattgaaga agaactggcc
    28081 tggcattata taatgcttgc ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat
    28141 tatcaaaaaa atagtctaat gggatttctt tactggcata tctgctgcta tcgcagaaag
    28201 ttaacggatg ctgaacttga agcgtgttat cgttacgaca agcagttttg ggagcactac
    28261 agtaagaaat gccgttggcc cataagaaat gttccctggg gagcatcatc cgttaaatac
    28321 tcataaacgc ctgaaatttt gccgggggcg ctgcgcttgc ccggcctaca ttagagcgta
    28381 actgcaatga attgtgccca tctgtaggcc tgataagcgt agcgccatca ggcatttaca
    28441 acttaaatca caccctgcgc cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc
    28501 gcgccctgca cgtagttggt ttgctcacct tcaccaccat gctcaacaca ggcatggtgg
    28561 atatccagca tgatgtgatg caaacgtgcg tcaactttct cggctttcca gcccaggcgc
    28621 gcagcgtttt gtgccatttc caggcccgac gttgctacac cgccagcatt agccgcttta
    28681 cccggtgcaa acagtacgcc tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc
    28741 atatttgccc cttcagcgac ggctttaacg ccattggcga taagctgatg cgcggtgtca
    28801 acatccagtt cgttctgggt ggcgcaaggc agggcgatat caaccggtac agaccacggc
    28861 tgttggcctt cgagatagac cagaccaaat tctttggcgt aatctgccac tcgaccatcg
    28921 cggctggctt tgatttcgat aagacgtgcc agtttctctt tcgtgaatcc gctttcatca
    28981 actacagtgc cgctggagtc tgacgcagtg atcgcacgag caccaaattc catcgctttt
    29041 tcgatagcgt actgggcgac gttgccggag ccagaaacgg aaacgcgcat cccttcaaaa
    29101 cccataccgt ggcgttttaa cattgcttct gtgaaataaa ccagaccgta gccggtagct
    29161 tccgggcgaa taagactacc gccaaatgaa aggcccttac cggtgaagac gcaggcggta
    29221 ttgttggaga gctttttcat catccccgcc ataaagccga cttcacgacc accaacgccg
    29281 atatcacctg ccggaacgtc ggtatccgcg cccaggtggc gatacagttc agtcatcagc
    29341 gcctggcaaa aacgcatcac tttaccttcg ctttttcctt tcggatcgaa atcgctgccg
    29401 cctttaccac cgcccatcgg cagggtagtc agggcatttt tgaaggtttg ttcaaagccg
    29461 aggaatttga gaatggaaag gttaactgac ggatggaagc gcataccgcc tttgtacggg
    29521 ccgatggcag agctgaactg cacacgccat gcacggttga cctgtatctg gttgcgatca
    29581 tcaacccata ccacgcgaaa ctggatcacg cgctccggtt caaccagacg ctccagtaat
    29641 gacatctggc gatattttgg attttgttca agaaaaggcc agagtgtggt cattacttca
    29701 cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct tttggacatg gttgaggaat
    29761 gactccagag aatatgtctg atccatagat ataaaaacct tatatattaa tacgattgtg
    29821 cttatgttgt ttatgtcatg tgcttttgca gttttcgact ataccatcaa gaatgtgacg
    29881 gtaagcaaga aaaaaatgac ccaggaaagc aaattaataa cgagagtaat ctcataacca
    29941 aatgcgataa ctcagacgct aaagttttgc cacccggcta ccgttatcag tagcagagac
    30001 aataacgtta aggagttccc tgtgagtcga gcattgttcg ccgtagtatt agcatttcca
    30061 ttaattgcac tggctaaccc gcattatcgt ccggacgtgg aggtaaacgt accgccagaa
    30121 gtgttcagtt caggcgggca gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat
    30181 caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa tactgctgca atgccagcgt
    30241 gatgacaaaa cccttagcac taatccgctg ctctggcggc gcgtaaagcc ataaacgcct
    30301 ctaataatgg aatgtcagca ggggccagcg gatattgcag cgcctcttca ggtgagcacc
    30361 agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg
    30421 catgaagatg gataatccgg cccgaaactt ctcgctgatg gctggcaaca tactcaccca
    30481 cagttgcttc gatgtccaat tcttcgttta actcacgcac cagcgcctgc tgctggcttt
    30541 catccagctc gactttacct ccggcaaact cccataatcc cgcctgatcg ctctgggcgg
    30601 ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat ggcggcaaca acttcaatca
    30661 ttttcataaa ttacacacgg gcagataagc gaaaaatgcc ataatatccg cactgttgct
    30721 caatcagaaa ggctttatat ttaaggagtg cctgtgctag accgccatct tcatccccgg
    30781 attaaaccgt tgttgcatca gtgcgtgcgg gttcttgata aatcgggcat tacgccagat
    30841 ggtttaacat tagtgggatt tgccatcggc gtgctggctc tgccgtttct ggcgctgggc
    30901 tggtatctgg cggcgctagt cgtcattttg ttgaacaggc tgcttgatgg tctggacggt
    30961 gcgctggcgc ggcgcagagg gcttaccgac gcgggcggct ttcttgatat ttctctcgat
    31021 tttctctttt acgcgctggt gccgtttggc tttattcttg ctgcaccgga gcaaaatgcg
    31081 ctggcaggcg gctggttgtt gtttgcgttt atcggcacgg gcagcagttt tctcgccttt
    31141 gctgcactgg cagcaaaaca tcagattgat aaccccggtt atgcacataa gtcgttttac
    31201 tatctggggg gattaaccga aggcacagaa acgatcttac tgtttgtgct gggatgttta
    31261 ttccctgcat ggtttgcgtg gtttgcgtgg atcttcggtg cgttgtgctg gatgacgacc
    31321 tttacgcggg tgtggagtgg gtatctgacg ctgaagtcgc tccagcgtca gtaattgcaa
    31381 tgttatttgc tactgcccgg gccgcgttca ccggttgcaa ccggattttt tggatcgctg
    31441 ctccattcgt accagccgcc gtcatacacg gaaacattct tccaacccat ggcgcgtgcg
    31501 tacataaagg tttcggacgc gcgccagccg gtgccgcagt agaatgaaac ttgctgatct
    31561 ggtttgatat tccatgcttt ccacatagcg gtaatatcat cggcgctacg catggtgcca
    31621 tccgggttat ggaaatcttc catatgcgtc gagtcgctac cagcgtgtcc ccaacgtgct
    31681 ccggctattt caccttttgg tttaatatag ctgtaaccgc tggtcgtacc gataaattct
    31741 ggccacgaac gaatgctcac cagcgatgca tcctggcgat gcagcagtcc acgggcctgt
    31801 tccatatcga gcatcaactg cggttgtgcc gggatcttca cgccgaaatc tggttccgct
    31861 ttcactttcg gtggcgttcc tcgctcaaca ggcagtcccg cgtcggacta ggtttgccag
    31921 ccgccatcca gcagacgcac atctttcacg cctgcgtaga gcataatttg cgccacacgc
    31981 gctccagcgt atacgtcacg cccgtacaga atgaccgtgg tgtcatggcg aatgccgtgt
    32041 tttgccagca tcgcttttag ttgttcatca gaaactttgt tccacagcgg ttcgctttcc
    32101 acttcgttgg tatcgatgta gtcagcgcca ggaatatggc tgataaggta aagcttagga
    32161 gcgccccagg ccgcttcaat gactttccag ccaccgacag gtttcgccgt aacctcttta
    32221 ccttgttgca ggtcatgcag ccattgcgga taaaccagct gctcaaagtg cggcagtttt
    32281 tgtagacggg aaggttcgct tagcgtgtca ctcagaatgg agatatgcgt aaaacctgct
    32341 ttttgcagtc gcgttttgac ggcgtcgaca tctttgccat taccgtacag cgccaccggg
    32401 gcatcggctt tcaggttatg ttgcttgatc cacgcgttga gctgttcggt gctcattttg
    32461 tcgagccagc tagcagagag gtttaaggcg gcaggttcat gaccagaagg gccatttaag
    32521 gtttgtggcc agccgttata aaacgcgctg gggcgagtat ctatcgcttt gccattttgt
    32581 tgctgaagtt ggtcaagtgt aagaggcttc gccagttcag cggcccacga agaagcgcaa
    32641 gccagcccta aagccattgc cagcgcggtc atttgagaaa cacgtttcat cgaaaatccc
    32701 gacattatag ttttgagtgc gccattctga actgacgcat tgagaaaaac tttgcgttat
    32761 cgcagtttgt tgtaattttc tgaccactgc gccatatcca gaacagaact atcagcagga
    32821 acatcctgga gatcgtgcgt tacctgaacg acggggatcg ccagggcgcg aacttcgctg
    32881 aacacccact ggcgaaaatt atcgcgcaga gccacatcaa gacggctgaa tggctcatcc
    32941 aggagtaacg cttttggctg ggcgagaagg gcgcgtagta gagcaacgcg cgctcgctga
    33001 ccgccagaca aagtggcagg atcctgatgg aaagctccgt caaggcctga acgctcaagt
    33061 gcatcattca cggcattacg tcgggcattc cccttaagtg tcgccggtag cgccagcagt
    33121 aaattttgcc cgacgctgaa ctggtcaaat aacagtgcat cctgaaaaag aatgccaatc
    33181 tgacgctgtg cggtgggtag catgtcaatc cgttgctcat tgagccatag ctcacctgta
    33241 caagaaaact gtccggccag tgcaccaatc atccatgaaa acagagtgga ttttccacag
    33301 ccagatggcc ccattaacgt gacgatgtca cctttatcaa ccgtaaagtt aacgtttgtc
    33361 agcaagcggc tttctggtaa acgtagcgaa acatttttca cgcagagcat tagcggattc
    33421 cttgtctgac ataacctacc cattttgcga ctaacgcggt cagggcaaaa ataataagcg
    33481 gtaacaacag ttgccataaa gcctgggcgg cgagaatacc gttgctgccg ccgctgctta
    33541 atgccaccgc ctcagtggtg agcgtcggaa aacgccccgc gcccagccac agcgtcggca
    33601 tatactgcgc aatactgact gaaaatccca ccgcgaaggc aatcagcaca gggcgcaaca
    33661 tgagtgggca tttcacgtaa aagaagattt tggcccgcga ccagcccagt gtttgcgcaa
    33721 tcaaaattaa ccgtgaatca atgcgctgcc aggcgggttg caggataaac agcatccacg
    33781 gcattaccca cagcagatgc ccccagacca ccgccgtcca gcttccatcc aggttcagcc
    33841 atagcgccag cgtgtactgg cccgccacca gtggcagagc gggcaataga atgggcaacc
    33901 atagccacaa ctggcgacgc tgtgcccccc attccagcca cagcagtaac aggagcaaag
    33961 cgatgaatgt tgctaccagc cccattgtca gactgttgat gagtgcttca ctattgatcg
    34021 tcgactgatc cgcgaggatc gccagcagaa ccacacacag cacaccggtt aagggtaaaa
    34081 aactcgccag cgtagtgcct ggtaataaag gtgtggcagg cttgcgaatg ccatctacgc
    34141 ggggaatagt gcgccgccag ctgcgccata gcagatagct cagcaaaacg taggcggcga
    34201 gtaacagcat cagcagcaga ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct
    34261 gagttaacca ctgccagcta attactgcca gcgtcggtgg attacctggc ccaagaataa
    34321 tcgccacatc cacgaccgaa agcgaccagg caacaatcgc cagcatcgcc attgccagcg
    34381 aaggcgcgac ggagggcaac agcagccagt tcaggcattg ccagcggctg tagcccagcg
    34441 aatccagcac aatgacctgc tgcaacagcc gtttttcgct caatactgcc actaagatcc
    34501 acagcagaaa tgcgctttct ttcactgcaa gggtgaggcc cagaccgatc caaatcggtc
    34561 cattggcgga gtgaaatacg ggaaatagtc atatagcagc cctccgtcag caaagagcag
    34621 cagggcgctg gtggcaaaag ccacatgggg aatggcgagc agccacggca gacgggcgca
    34681 catacgctgc catttcggcc caggccatag cgccacaatc accagcaggg caatcaacaa
    34741 tgccccgacc gccacgatgg ttgtcgacac cagcgttgcc agtaatgcct gcggtaactg
    34801 tggatcggca aataacgcct gccagcgtgt caacgacaac gcaggcgata tcagcgtgag
    34861 agctgccggg atcagcggtg cataaatcac cgccaccatc gcccacagca aaaagattaa
    34921 tgcgtaccgt aacggcgtaa ccattcttgt tccagtgcat tcacccatcc tgcgtgcggt
    34981 tcagccagta ccggcggcag atcctgcggc attcttgatt gcaatatttc gcgctgcccg
    35041 tcaggcagtt tttgcggatc gagaacagaa ggatcgcccc agacaacggg atctgcttta
    35101 cgcagttgcg catcgggtga gagcaggaaa ttggcaacta ccttcgccgc agcactggca
    35161 ttcgcgttgg ctggaatggt gacgaaatgc acgttgccaa tcatcccctc gcgaaagcca
    35221 aaactgtaac tgcttgccgg caaatcgccg ctggcgattt tttgctgcgc atgcgcgggg
    35281 ttaaaggtca gcgacaggcg caatgtgccg gctttcagca gagcatccat ccgcgcgggc
    35341 gaaggcggga aatcctttcc ttcgcgccac aaatacggat gcagtgcatc aagatattgc
    35401 cacaagggcg cagtgacacg ggcgaaagtc gcatcgtccg gcgcttcttt taatgcggcg
    35461 ggatctggcg tcagcataat cagcaactgt tcaagaaacg ccgtgccggt aaagtccggt
    35521 gggcgcggat aagtaaccgt gccgggattg gctttagcaa actccagtaa ggcttgcggc
    35581 gtttgtggtg gctgtggtaa acgtcagttg tgcgccgccc cagggcgatt ccgcaccttg
    35641 tgtcggcact gaaaaatctt cccgcaccgg cagctgtgtg tcgacatagc gccagttggg
    35701 cagagtctcc gcccagcccg tttgtaataa attggcctct ttta
//
LOCUS       DADLZM010000026        35272 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLZM010000026 DADLZM010000000
VERSION     DADLZM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.026"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="JNY73_001262"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="JNY73_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY8047336.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="JNY73_001263"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="JNY73_001263"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY8047337.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="JNY73_001264"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="JNY73_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY8047338.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNY73_001265"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNY73_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY8047339.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="JNY73_001266"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="JNY73_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNY73_001267"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNY73_001267"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY8047340.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNY73_001268"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNY73_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY8047341.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="JNY73_001269"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="JNY73_001269"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY8047342.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="JNY73_001270"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="JNY73_001270"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY8047343.1"
                     /translation="MRFDTVIMGSGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNKENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQITQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="JNY73_001271"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="JNY73_001271"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY8047344.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13529
                     /locus_tag="JNY73_001272"
     CDS             12630..13529
                     /locus_tag="JNY73_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY8047345.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13548..13769
                     /locus_tag="JNY73_001273"
     CDS             13548..13769
                     /locus_tag="JNY73_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047346.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQQGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13810..14280)
                     /locus_tag="JNY73_001274"
     CDS             complement(13810..14280)
                     /locus_tag="JNY73_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY8047347.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14335..15537)
                     /locus_tag="JNY73_001275"
     CDS             complement(14335..15537)
                     /locus_tag="JNY73_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY8047348.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15624..16166)
                     /locus_tag="JNY73_001276"
     CDS             complement(15624..16166)
                     /locus_tag="JNY73_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047349.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16445..16870
                     /gene="nudI"
                     /locus_tag="JNY73_001277"
     CDS             16445..16870
                     /gene="nudI"
                     /locus_tag="JNY73_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY8047350.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16909..17511)
                     /locus_tag="JNY73_001278"
     CDS             complement(16909..17511)
                     /locus_tag="JNY73_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY8047351.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17819..18958
                     /gene="arnB"
                     /locus_tag="JNY73_001279"
     CDS             17819..18958
                     /gene="arnB"
                     /locus_tag="JNY73_001279"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY8047352.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18962..19930
                     /gene="arnC"
                     /locus_tag="JNY73_001280"
     CDS             18962..19930
                     /gene="arnC"
                     /locus_tag="JNY73_001280"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY8047353.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19930..21912
                     /gene="arnA"
                     /locus_tag="JNY73_001281"
     CDS             19930..21912
                     /gene="arnA"
                     /locus_tag="JNY73_001281"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY8047354.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYDS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21909..22799
                     /locus_tag="JNY73_001282"
     CDS             21909..22799
                     /locus_tag="JNY73_001282"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY8047355.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22799..24444
                     /gene="arnT"
                     /locus_tag="JNY73_001283"
                     /pseudo
     CDS             22799..24444
                     /gene="arnT"
                     /locus_tag="JNY73_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
     gene            24441..24776
                     /gene="arnE"
                     /locus_tag="JNY73_001284"
     CDS             24441..24776
                     /gene="arnE"
                     /locus_tag="JNY73_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY8047356.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24776..25162
                     /gene="arnF"
                     /locus_tag="JNY73_001285"
     CDS             24776..25162
                     /gene="arnF"
                     /locus_tag="JNY73_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY8047357.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="JNY73_001286"
     CDS             complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="JNY73_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY8047358.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25533..26888)
                     /gene="menE"
                     /locus_tag="JNY73_001287"
     CDS             complement(25533..26888)
                     /gene="menE"
                     /locus_tag="JNY73_001287"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY8047359.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26885..27847)
                     /gene="menC"
                     /locus_tag="JNY73_001288"
     CDS             complement(26885..27847)
                     /gene="menC"
                     /locus_tag="JNY73_001288"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY8047360.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFTKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27847..28704)
                     /gene="menB"
                     /locus_tag="JNY73_001289"
     CDS             complement(27847..28704)
                     /gene="menB"
                     /locus_tag="JNY73_001289"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY8047361.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDICYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28719..29477)
                     /gene="menH"
                     /locus_tag="JNY73_001290"
     CDS             complement(28719..29477)
                     /gene="menH"
                     /locus_tag="JNY73_001290"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY8047362.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29474..31144)
                     /gene="menD"
                     /locus_tag="JNY73_001291"
     CDS             complement(29474..31144)
                     /gene="menD"
                     /locus_tag="JNY73_001291"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY8047363.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31233..32528)
                     /gene="menF"
                     /locus_tag="JNY73_001292"
     CDS             complement(31233..32528)
                     /gene="menF"
                     /locus_tag="JNY73_001292"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY8047364.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAKGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGERLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="JNY73_001293"
     CDS             complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="JNY73_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY8047365.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32967..33428)
                     /locus_tag="JNY73_001294"
     CDS             complement(32967..33428)
                     /locus_tag="JNY73_001294"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8047366.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDCIPHIGMAREVIQA"
     gene            33493..34410
                     /gene="rbn"
                     /locus_tag="JNY73_001295"
     CDS             33493..34410
                     /gene="rbn"
                     /locus_tag="JNY73_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY8047367.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34601..35272
                     /locus_tag="JNY73_001296"
                     /pseudo
     CDS             34601..35272
                     /locus_tag="JNY73_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8673 a         9421 c         8716 g         8462 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa gggatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgaggaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgtt ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgagcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgctgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcagc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa taaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgcagatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attacccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gagataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ccttgcgcat gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca
    13501 ctgatgctga tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg
    13561 aacggctacg gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc
    13621 agcaaggtaa attagaaggt ttgcagcaag gtatgcatga acaagccatt aaaattgctt
    13681 tgcgcatgct ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg
    13741 aagccgatct ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg
    13801 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
    13861 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
    13921 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
    13981 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
    14041 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc
    14101 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
    14161 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
    14221 gcgcgcagcc cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa
    14281 attttgccgg agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac
    14341 ggataaagtc atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc
    14401 gtttaaccaa cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc
    14461 caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc
    14521 tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa
    14581 atggttggcc cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga
    14641 aggaatcact tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa
    14701 accaccggtg aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact
    14761 gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt
    14821 aacgtccagc cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag
    14881 ttcgatgatc ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt
    14941 gtccaggctt tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac
    15001 cggcggctgt ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat
    15061 caccttaaat tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac
    15121 ggcaaaacca caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc
    15181 cgcttgttta cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat
    15241 ctctttgagc caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag
    15301 tgcgcttaaa tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg
    15361 ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg
    15421 gcgagataat ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc
    15481 aacgatttgc ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt
    15541 tatctcctgc ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa
    15601 actgaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca
    15661 gccacagcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    15721 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    15781 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    15841 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    15901 ttcgggttgg tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    15961 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    16021 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    16081 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    16141 agacctgcta gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc
    16201 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    16261 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    16321 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    16381 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    16441 ccctgtgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    16501 gtgcaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    16561 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    16621 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    16681 gacatatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    16741 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    16801 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    16861 tcttctttaa caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac
    16921 gaattcccca tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata
    16981 atcaggttta aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    17041 ggtgaaaata acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    17101 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    17161 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    17221 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc
    17281 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    17341 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    17401 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    17461 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    17521 acctgtagac aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa
    17581 ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc
    17641 caaatgcaaa gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag
    17701 tttcttaagg ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac
    17761 acaacactct ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat
    17821 gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa
    17881 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc
    17941 cttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
    18001 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
    18061 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
    18121 tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg
    18181 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg
    18241 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
    18301 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat
    18361 taaaaatatt acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg
    18421 ccagctacgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg
    18481 gggccgtgca ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat
    18541 taacgccgcg attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg
    18601 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
    18661 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
    18721 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
    18781 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
    18841 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
    18901 gactaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc
    18961 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
    19021 ggaaagctta ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga
    19081 gtatgagatc ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga
    19141 agcctcacaa gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca
    19201 acattcagcg attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga
    19261 tgccgatctc cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg
    19321 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc
    19381 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg
    19441 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
    19501 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt
    19561 acatcatgcc gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt
    19621 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg
    19681 cagcattatt gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac
    19741 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
    19801 tattggcgct cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac
    19861 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
    19921 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
    19981 gctctgctgg ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt
    20041 gaaaaagcct tttatgattc ggtggctcat ctggcggcgg aaagagacat tccggtttat
    20101 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag
    20161 gtgattttct ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc
    20221 gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
    20281 aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa
    20341 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
    20401 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
    20461 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
    20521 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
    20581 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
    20641 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
    20701 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
    20761 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
    20821 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
    20881 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
    20941 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
    21001 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
    21061 tatcacgtca aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa
    21121 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
    21181 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
    21241 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
    21301 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
    21361 ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga
    21421 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
    21481 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
    21541 actgatatcc gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc
    21601 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
    21661 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
    21721 gcgggctttc gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag
    21781 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
    21841 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
    21901 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
    21961 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
    22021 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
    22081 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
    22141 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
    22201 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
    22261 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
    22321 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
    22381 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc
    22441 cgttccgtcc actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
    22501 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
    22561 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag
    22621 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
    22681 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
    22741 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
    22801 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
    22861 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
    22921 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
    22981 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
    23041 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
    23101 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
    23161 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg
    23221 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    23281 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    23341 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    23401 ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct cccctgggga
    23461 ctggcgatag cgcagcggga gcctgacttg tggcactatt ttttctgggt tgagcatatt
    23521 caacgctttg cactagatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    23581 atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta cacaggctgg
    23641 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    23701 tttttctccg tcgctaaagg caaattgccc acctatattc tttcttgctt tgcacctctg
    23761 gcaatgctgt tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    23821 attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac atttgtgatc
    23881 tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    23941 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    24001 gaaaagacct ggtctttcgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    24061 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    24121 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    24181 gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    24241 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    24301 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    24361 gatatcaaca gtctcgccat tccgcccgca gatgccatcg ttcgtcagga gcgtctggtg
    24421 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    24481 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac aaacggcgca
    24541 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    24601 ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta agtctgaatt
    24661 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    24721 ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg
    24781 cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    24841 tgcggcaagt catctgccgc cgatgacgca cttatgggat tttatcgcgg ctctgttagc
    24901 ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    24961 ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    25021 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    25081 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    25141 cacgacaaaa caacgttact gagttttccc tgccgctgta cactgccgtt cccactcatc
    25201 ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac gattaataca
    25261 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    25321 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    25381 atcttgttta ttgcagcacg attttttgtg ccagccattc cattgcatta tcctgtttgc
    25441 taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa
    25501 tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt
    25561 agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa agtcagccag
    25621 cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag atcaacggtt
    25681 tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc ggcaacggaa
    25741 acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac ttcttccggc
    25801 tgaataccct caccgccact gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg
    25861 ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa
    25921 accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca
    25981 ttattaacga ttttcacttc ccgacccggc agcggcaaac caacgtctgc caggccgtcg
    26041 gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga
    26101 atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac
    26161 accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg tgttggcacc
    26221 agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgcaccgtc
    26281 atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc
    26341 ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc
    26401 gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggcaaacc ggtagaaccc
    26461 gaggtcaacg tcattgagca cagacgcgtc ggctgccacg cagcggcatg tacgccttca
    26521 accagctgaa tgtgcagcga cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc
    26581 gcaaattgca gcgtcagatt gggcagcaat tcttcaagca acggttgcgg cagctgaggg
    26641 ttcacgggca acacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc
    26701 ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccacaccctg cgccgcaaag
    26761 cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta
    26821 agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg ccagtcagag
    26881 aagatcataa caactgctcc agtacatcag cgtccactaa cggcagtggg ctccccggcc
    26941 agcgacgaac ctgctgcgcc tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg
    27001 gcgttaacca ggcagcaatc cgcgccagtt gcgttagacc taagctcgac tcaatggaag
    27061 aactgatcac cgccgtcagc cccagcgcat gcgccgcctg tacctgctcg cgtacttttt
    27121 ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa
    27181 aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg
    27241 caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtggc
    27301 gataatccgg gttaacatat ttggtaaact gctgaccttt cagcggtgtc caggcgcgat
    27361 ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagattc accaccatgc
    27421 cgtcgcgcac cgcttcgtac aatcccactt tgaccttcgc cactttctcg cccggcatat
    27481 cggcaagttt gaggatcaga tcgtccggat cgccattaca cagcggtgcc gcacggtagt
    27541 tggctgcttg cggcaacgta tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca
    27601 cggaaggcat ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca
    27661 gcacgctttg cgcctcttcc cagttttcct ggctgaagcc cggcagtggg gagatctccc
    27721 cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc
    27781 gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc
    27841 tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct ggttgaaagc
    27901 gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag
    27961 ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag
    28021 cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg acggtttctt tttccagatc
    28081 cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgttttg cgtcgtactg
    28141 acggcacagg aaccagattt ctcgcgcttt tttctgcccg acgatacgag ccatgtagga
    28201 agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt
    28261 atctgctgcg atagtcaggt cgcacatcat gtgcagaacg tgaccaccgc cgatggagta
    28321 gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag
    28381 cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt
    28441 ctggtcacca ccggagcaga acgctttatc gcctgcgcca gtcagaatga tcacgccgat
    28501 attgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg tcagaggacg
    28561 gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccgt cggtggattt
    28621 ttcataacaa atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaagg
    28681 cattgcttca tcaggataaa tcatattgtg tcctttagtc aaaaacgcaa gatctgcgcc
    28741 agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg
    28801 acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc acgttcacca
    28861 cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg
    28921 gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt gctgcgcagc
    28981 gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg ttgataccag
    29041 tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa
    29101 cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgataata
    29161 accccacaaa gccccgccaa cccctggcaa gccgccatca tcgccacccg tccgccaagc
    29221 gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag
    29281 tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg acctgggaga
    29341 tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa
    29401 tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc
    29461 tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac
    29521 catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg
    29581 caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg
    29641 cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt
    29701 gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca
    29761 gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt gcggagagat
    29821 cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa
    29881 gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct gccggaagtt
    29941 gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat tgcccctgtt
    30001 caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg
    30061 caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc
    30121 gtttttctgc cggatgcagc tccagccagt cggcaatatt gccaattaag cgacgtccgc
    30181 gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac tcttctggtt
    30241 cacagcttgc ctgccattgc aggagccgtt tccccgtcag gctgcttccc agttgcacca
    30301 caatttgtgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac
    30361 acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt
    30421 gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc
    30481 cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga cgaggcgctt
    30541 cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc
    30601 cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc
    30661 ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat
    30721 cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct
    30781 ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga
    30841 gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg
    30901 ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg
    30961 ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg
    31021 ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc
    31081 cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg
    31141 acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta
    31201 ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcagc
    31261 cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg
    31321 cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg
    31381 cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg
    31441 aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct
    31501 gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag
    31561 atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc
    31621 agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag catgttctcg
    31681 cgctggtttt tatcatccgc catcagcctc tctcctaact gctgcgcctg cttatcatca
    31741 ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc
    31801 cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa
    31861 tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga
    31921 cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc acctttggcg
    31981 atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt
    32041 tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata
    32101 aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcaaccgc
    32161 agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc
    32221 gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga
    32281 agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacggcagct
    32341 tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg actggccagc
    32401 cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg
    32461 ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt
    32521 gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa
    32581 atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc
    32641 ccgacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta gtcatcagca
    32701 cgataaacag cctgcttcgc ccgatagtaa taactgtctg aagcctggct aacccgtttt
    32761 ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcggcg
    32821 ggctcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt caggtcgtca
    32881 tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt
    32941 cactaactat agacaagagt tgttgattac gcctgaatta cctcgcgtgc catcccaatg
    33001 tgtgggatac aatcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag
    33061 aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgta
    33121 catgtttcga gtgttttact catcagctgc tgccccactt tttcaccacg caacgcttca
    33181 ctgacaatca cccgacctat aacgaccggc tcaagatcat catcactttt cagaatcctc
    33241 gcatacgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa
    33301 tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc
    33361 agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat
    33421 tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata ttttttgaaa
    33481 cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg
    33541 cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg
    33601 tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa
    33661 ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg
    33721 ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt
    33781 atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga ttatccgctg
    33841 gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct
    33901 tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca tgataaaccg
    33961 ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc actgtttcag
    34021 gaattaaaag cgggcaaaac aatcacgctg gaagatggaa ggcagattaa cggcgcagat
    34081 tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc
    34141 gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat
    34201 ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca
    34261 ctagcccgtg aggctggagt cggcaagcta atcattaccc acgtcagctc gcgctatgat
    34321 gacaaaggtt gtcaacacct gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg
    34381 gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac agggtgaaac
    34441 ttgccctgtt atagcaaccc gcgctacata tatccaccca cacaggcata ttgagcgatt
    34501 tttgatattt attcatctcc ttacgcacta tattcagagc aataagttgc ctcttttcat
    34561 cttgattcag cacaaccttt gcaactaaaa ggagcccatg atggttacag ttgtcagcaa
    34621 ttattgtcaa ttatctcaaa cccaactcag tcagacattt gcagaaaaat ttactgtgac
    34681 cgaggaatta ctgcagtctt taaaaaaaac agcgttatcc ggagatgaag aaagtattga
    34741 gttactgcat aatattgcgt taggttatga tgaatttgga aaaaaagcag aagatattct
    34801 ttaccatatt gttagaaacc caacaaatga gacctatcga ttatccgact tattaaaaat
    34861 gcctgtttaa aattatataa tttggcacat accgcaacca actcccacct taaaccaact
    34921 ggtccggata attcagatgt tctcctgttc aaaaagctat tctccccttc gaaattaatg
    34981 acaattatcg gtaatgaaat tcctcttata tctgaaaaac agtcgctttc aaaggtgctt
    35041 ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa aaatcgtcaa
    35101 ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc aaaaaatact
    35161 caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca cctggaagac
    35221 gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca gt
//
LOCUS       DADLZM010000027        35253 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLZM010000027 DADLZM010000000
VERSION     DADLZM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.027"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1152)
                     /gene="setB"
                     /locus_tag="JNY73_001297"
     CDS             complement(<1..1152)
                     /gene="setB"
                     /locus_tag="JNY73_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY8047368.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1520..2650
                     /locus_tag="JNY73_001298"
     CDS             1520..2650
                     /locus_tag="JNY73_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY8047369.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2650..3588
                     /gene="fruK"
                     /locus_tag="JNY73_001299"
     CDS             2650..3588
                     /gene="fruK"
                     /locus_tag="JNY73_001299"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY8047370.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3605..5296
                     /gene="fruA"
                     /locus_tag="JNY73_001300"
     CDS             3605..5296
                     /gene="fruA"
                     /locus_tag="JNY73_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY8047371.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5720..6661
                     /locus_tag="JNY73_001301"
     CDS             5720..6661
                     /locus_tag="JNY73_001301"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY8047372.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6649..7587
                     /locus_tag="JNY73_001302"
     CDS             6649..7587
                     /locus_tag="JNY73_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY8047373.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7681..8931
                     /locus_tag="JNY73_001303"
     CDS             7681..8931
                     /locus_tag="JNY73_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY8047374.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9003..9701)
                     /gene="yeiL"
                     /locus_tag="JNY73_001304"
     CDS             complement(9003..9701)
                     /gene="yeiL"
                     /locus_tag="JNY73_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY8047375.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLT
                     LEMDPENKFSGMMQ"
     gene            9832..10773
                     /gene="rihB"
                     /locus_tag="JNY73_001305"
     CDS             9832..10773
                     /gene="rihB"
                     /locus_tag="JNY73_001305"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY8047376.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10873..12123
                     /locus_tag="JNY73_001306"
     CDS             10873..12123
                     /locus_tag="JNY73_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY8047377.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVIGILIRILGGIFQKALNISKIESFVAVTTIFLVQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12179..13263)
                     /locus_tag="JNY73_001307"
                     /pseudo
     CDS             complement(12179..13263)
                     /locus_tag="JNY73_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(13266..14123)
                     /gene="nfo"
                     /locus_tag="JNY73_001308"
     CDS             complement(13266..14123)
                     /gene="nfo"
                     /locus_tag="JNY73_001308"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY8047378.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14197..15246)
                     /locus_tag="JNY73_001309"
     CDS             complement(14197..15246)
                     /locus_tag="JNY73_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY8047379.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15345..16226
                     /locus_tag="JNY73_001310"
     CDS             15345..16226
                     /locus_tag="JNY73_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8047380.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16432..17901
                     /gene="lysP"
                     /locus_tag="JNY73_001311"
     CDS             16432..17901
                     /gene="lysP"
                     /locus_tag="JNY73_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY8047381.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18193..20184
                     /gene="cirA"
                     /locus_tag="JNY73_001312"
     CDS             18193..20184
                     /gene="cirA"
                     /locus_tag="JNY73_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY8047382.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20216..21052)
                     /gene="yeiG"
                     /locus_tag="JNY73_001313"
     CDS             complement(20216..21052)
                     /gene="yeiG"
                     /locus_tag="JNY73_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAY8047383.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATYYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21310..21978
                     /gene="folE"
                     /locus_tag="JNY73_001314"
     CDS             21310..21978
                     /gene="folE"
                     /locus_tag="JNY73_001314"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY8047384.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21995..23152
                     /locus_tag="JNY73_001315"
     CDS             21995..23152
                     /locus_tag="JNY73_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY8047385.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23294..24262
                     /gene="galS"
                     /locus_tag="JNY73_001316"
     CDS             23294..24262
                     /gene="galS"
                     /locus_tag="JNY73_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY8047386.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNTNAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRAATVLCRR"
     gene            24613..25611
                     /gene="mglB"
                     /locus_tag="JNY73_001317"
     CDS             24613..25611
                     /gene="mglB"
                     /locus_tag="JNY73_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY8047387.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25672..27192
                     /gene="mglA"
                     /locus_tag="JNY73_001318"
     CDS             25672..27192
                     /gene="mglA"
                     /locus_tag="JNY73_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY8047388.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27208..28218
                     /gene="mglC"
                     /locus_tag="JNY73_001319"
     CDS             27208..28218
                     /gene="mglC"
                     /locus_tag="JNY73_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY8047389.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28254..29489)
                     /gene="preA"
                     /locus_tag="JNY73_001320"
     CDS             complement(28254..29489)
                     /gene="preA"
                     /locus_tag="JNY73_001320"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY8047390.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29483..30722)
                     /locus_tag="JNY73_001321"
                     /pseudo
     CDS             complement(29483..30722)
                     /locus_tag="JNY73_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(30916..31155)
                     /locus_tag="JNY73_001322"
     CDS             complement(30916..31155)
                     /locus_tag="JNY73_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY8047391.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31158..31877)
                     /gene="sanA"
                     /locus_tag="JNY73_001323"
     CDS             complement(31158..31877)
                     /gene="sanA"
                     /locus_tag="JNY73_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY8047392.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32027..32911)
                     /gene="cdd"
                     /locus_tag="JNY73_001324"
     CDS             complement(32027..32911)
                     /gene="cdd"
                     /locus_tag="JNY73_001324"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY8047393.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33041..33736)
                     /locus_tag="JNY73_001325"
     CDS             complement(33041..33736)
                     /locus_tag="JNY73_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY8047394.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEITASILPKSVTTPIAMSVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33733..34131)
                     /locus_tag="JNY73_001326"
     CDS             complement(33733..34131)
                     /locus_tag="JNY73_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY8047395.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34370..>35253
                     /gene="dusC"
                     /locus_tag="JNY73_001327"
     CDS             34370..>35253
                     /gene="dusC"
                     /locus_tag="JNY73_001327"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY8047396.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8662 a         8615 c         9306 g         8670 t
ORIGIN      
        1 cagagtggcg ataatcatca ccatcgcaaa ccagaacaca gcgtgataat tccagatctc
       61 ggcgacgatg cccgccactg atcctgcgat gatccagccc acgcgcgacg tgttggtata
      121 gagcgtggtg gctgaacctg cctgaccggg catcaaatcc tgaaaataga gcatcccgat
      181 gccgcccaga atgccaataa aaatagcatt tagcagctgc aagcccaaca gaatggcagg
      241 tgaatgcgcc atcagcattc ctgcgtaaaa acagacgcca cccacggcag caacgcgcat
      301 taagaaacgc ttacccagac gtttggcgaa atatccggca atcaacatcg tcgggatttc
      361 cagcccggcg gcggtcccca tcatcacacc ggccagtttc tcgggaagat gcagttcgtt
      421 gataataaat agcggcatgt tgatgatgta caggctgttc gagccccaca tcaatgtaca
      481 aatgacaaac agcagtagcg tatcgcgacg gttacggcgc ggcgcttcaa ccgtgccggt
      541 cgccagcgga agctcttttt gcatcgacgg taaaaacagc cacaccatca cgccgcaaac
      601 aataaacgct accgctgcgc tcagatacat taccgtaaag ctgaaaccca tcgctaacgc
      661 ataagccagc ggtgggccaa tgacccatgc cagtgaaacc tgagcgcgta aaaaagagct
      721 gaacatcacc gcctcacgtc cggttttgtc ggcatgttca cgggcaaggg caaacatttg
      781 cgggttagcg gtcgagccaa agctgctaag aaagacgcca acgaatagca aaacaaagta
      841 gttgcgattc caggcaaaaa gggtgcaggc cagcacgcct aacaggcagc aaaagacaat
      901 cagcgatttg cgatcgccgc gcttatcaga gcgcccagcg agaaactgac ttaccagaat
      961 cccaatgaca gcgctgccgg taaagaagaa tcccaccatc gccggacggg catgtacttc
     1021 atcggtaagg aaaatactga gtgtcggggt ttgcagagca cccgcaatac cggtgagaaa
     1081 ggcaacgatt aaaaacgccg tcgaggtcag gtcaaacgat ttcgcgctgg agactgcggg
     1141 ggagttatgc atgctggtcg atcactggaa gaggaagacg cggagtttac gctgcttgtc
     1201 agaaaataaa cagacagcga aatcaaaagc gccacgaata atcaaaatgc catttcactg
     1261 ttgctgattg gctgctgacg ctgctgaaat gaaattgctg ataacggatt ttcccatcag
     1321 caattaggaa aaatgacaaa aaattgtgca gcacatcaaa cttttgctca taactttacg
     1381 gctttccttg cgtgctgaaa cagaagtatt atgctttctt gaaacgtttc agcgcgatct
     1441 tgtctttaac cctaagccag gttggcgctt tttttctcat agaggctgaa tcgtttcaat
     1501 tcagcaagag aggagaacta tgttccagtt atccgtacag gacatccatc cgggcgaaaa
     1561 ggccggagac aaagaagagg cgattcgcca ggtcgctgcg gcgctggtgc aggccggtaa
     1621 tgtagcagaa ggctacgtca atggcatgct ggcgcgcgaa cagcaaacct caacgttcct
     1681 cggcaatggt attgctattc cacacggcac taccgacacc cgcgatcagg tgctgaaaac
     1741 cggcgttcag gtatttcagt tcccggaagg cgtcacctgg ggtgacggtc aggtagcgta
     1801 cgtggcaatc ggtattgctg ccagctcgga taaacatctg ggcctgctac gccagctgac
     1861 ccacgtactg agcgatgatt ccgttgctga acaactgaag tcagcaacaa cagcagaaga
     1921 acttcgcgca ttactgatgg gcgaaaagca gagtgagcag ctgaagctcg acaacgaaat
     1981 gctgacgctg gatatcgtcg ccagcgatct gctgactctt caggcgctga acgctgcgcg
     2041 tctgaaagag gcgagggcag ttgacgccac tttcgtcacc aaagccatca atgaacaacc
     2101 gctgaatctc ggacagggta tctggctgag cgatagcgcc gaaggcaatc tgcgtagtgc
     2161 gattgcggta agccgtgcgg caaatgcttt tgatgtggac ggcgaaacgg cagccatgct
     2221 ggtgagtgtg gcgatgaatg acgatcagcc catcgcggtt cttaagcgtc tcgctgattt
     2281 gttgctcgac aataaagctg accgcttgct gaaagcggat gcggcaacgt tgctggcgct
     2341 gctgaccagc gatgatgcgc cgaccgacga cgtgttaagc gcggagtttg tggtgcgcaa
     2401 tgaacacggc ctgcatgctc gtccaggtac catgctggtc aataccatta aacaatttaa
     2461 cagtgatatt accgtgacaa accttgatgg caccggcaaa ccggcaaacg gacgtagtct
     2521 gatgaaagtt gtggcacttg gcgttaagaa aggtcatcgc ctacgcttta ccgcccaggg
     2581 tgcagatgct gaacaggcgc tgaaagccat tggcgacgct atcgctgctg gtcttgggga
     2641 gggcgcataa tgagcagacg tgttgctacg atcaccctta atccggctta tgaccttgtt
     2701 ggtttctgcc cggaaattga acgcggcgaa gtgaacctgg tgaaaaccac cggtctgcat
     2761 gcggcgggta aaggcatcaa cgtggccaaa gtattaaaag acctgggaat tgatgtcacc
     2821 gttggcggct tcctgggtaa agacaatcag gatggttttc agcaactgtt cagcgagctg
     2881 ggcattgcca accgtttcca ggttgtacag gggcgcaccc gaattaacgt taagctgacg
     2941 gaaaaagacg gcgaagtgac cgacttcaac ttctcgggtt ttgaagtcac ccccgccgac
     3001 tgggaacgct ttgtgactga ttctctgagc tggctcggtc agttcgatat ggtctgtgtc
     3061 agcggaagct taccgtcagg cgtcagcccg gaagcgttca ccgactggat gactcgcctg
     3121 cgtagtcagt gtccttgcat tatctttgat agtagccgtg aagcgttagt agcaggtttg
     3181 aaagcggcac cgtggctggt gaaacctaac cgccgcgagt tggaaatctg ggcaggccgt
     3241 aaactgcctg aaatgaaaga tgtgattgaa gctgcacatg cgctacgtga acaaggtatt
     3301 gcgcatgttg ttatttcact gggtgccgaa ggcgcgcttt gggttaatgc ctccggcgaa
     3361 tggatcgcta aaccaccgtc agtcgatgtc gtaagcaccg ttggcgcagg ggattctatg
     3421 gttggtggcc tgatttatgg cttgctgatg cgtgaatcca gtgaacacac actgcgtctg
     3481 gcgacagctg ttgcagccct ggcggtaagt caaagcaatg tgggtattac cgatcgtccg
     3541 cagttggccg caatgatggc gcgcgtcgac ttacaacctt ttaactgaca gcaggagagg
     3601 cataatgaaa acgctgctga ttattgacgc taatctcggt caggcacgcg cctatatggc
     3661 gaagaccctg ctgggcgcgg cggcgcgaaa agcaaaactg gaaatcatcg acaatccgaa
     3721 cgacgcagaa atggcgattg ttctcggtga ttccatcccg aatgacagcg cgctgaacgg
     3781 taaaaatgtc tggctgggtg atatttcccg ggcagttgcg caccctgagc tgttcctgag
     3841 tgaagccaaa ggccatgcga aaccttacac tgcgccggtc actgcgacag caccggttgc
     3901 cgccagcggt ccgaaacgcg tagttgcggt gactgcttgc ccgactggcg tagcacacac
     3961 ctttatggcg gctgaagcca ttgaaaccga agcgaaaaaa cgtggctggt gggtgaaagt
     4021 tgaaacccgt ggttctgttg gcgcgggtaa tgcaatcact cccgaggaag tagccgcagc
     4081 ggatctggtg attgtggcgg cagatatcga agtggatctg gcgaaatttg ctggtaaacc
     4141 gatgtatcgt acctctaccg gtctggcgct gaagaaaacc tcgcaggaac tggataaagc
     4201 ggttgctgaa gcaacgccgt atgaaccggc gggcaaagct caaacggcga ccactgaagg
     4261 taagaaagag agtgcaggcg cttatcgtca cttgctaacg ggcgtctctt acatgctgcc
     4321 gatggtcgtt gctggtggtc tgtgtatcgc gctttctttt gcttttggta tcgaagcgtt
     4381 taaagagccg ggtacgttgg cagcggcgct gatgcagatt ggtggtggtt cagcctttgc
     4441 gctgatggtg ccggtactgg caggttatat tgccttctcc attgccgatc gtccgggcct
     4501 cacgccgggt ctgattggcg gtatgctggc ggtcagcacc ggttctggct tcattggcgg
     4561 tattattgcg ggcttcctgg ctggttacat tgcgaagtta atcagtacgc aactgaaact
     4621 gccgcagagt atggaggcgc tgaaaccgat cctgatcatt ccgctaattt ccagtctggt
     4681 ggtcggtctg gcgatgatct acctgatcgg taaaccagtt gctggcattc tcgaagggct
     4741 gactcactgg ctgcagacca tggggactgc gaatgcggtt ctgctggggg cgatcctcgg
     4801 tggcatgatg tgtactgaca tgggcggtcc ggtaaacaaa gcagcgtacg cattcggtgt
     4861 gggtctgctg agtactcaaa cctatggccc gatggcggcg attatggcgg caggtatggt
     4921 gccaccgctg gcaatgggcc tggcaacaat ggtggcgcgt cgcaaattcg acaaagcgca
     4981 gcaggaaggt ggcaaagccg ctctggtatt ggggctgtgc ttcatttcgg aaggtgcaat
     5041 tccgtttgct gctcgtgatc cgatgcgtgt gctgccgtgc tgtatcgtgg gtggggcgct
     5101 gactggcgca atctcaatgg cgattggtgc gaaactgatg gcaccgcacg gtggtctgtt
     5161 tgttctgctg atccctggcg ctattacgcc ggtattgggt tacctggtag caattattgc
     5221 cggtacgctg gtggcgggtt tggcctatgc cttcctgaaa cgtccggaag tggacgcagt
     5281 agcgaaagca gcgtaataaa aggtgtgtta cagggcagaa atttcctgcc ctgtaacatt
     5341 tgatgagcgc aaagagaatg accagcttgt gaatgtaatt ttatgaattt aaatggtttt
     5401 atctttcagt agaaacttca aacgttcatc aaacactaag gttatttcct caagggcaga
     5461 ttattttctg ccctttttta gtgttcctgt tctgtgtatc ccgtcacaaa ttcctcgctt
     5521 ttgtaattca ggcatttaat cgcattttgt gagcctgctc aaatctgccc attttgcctc
     5581 tctggcggta atttacttta cgtaaaacat gcatactatg agcacatgtt taaatatgaa
     5641 cacatgttta aaccgtcttt gcagtatcta tattcttgtg gattgacagg gttggtaatt
     5701 ttttaccaga ggacgtgcta tgcgcgaaaa ggattatgtc gtaattatag gttcggcgaa
     5761 tattgatgtc gccggatatt cacatgaatc attaaattat gcggattcaa atccaggtaa
     5821 aataaaattt acgcctggtg gagtagggcg caatattgca caaaacctgg cgttgctggg
     5881 taacaaagcc tggctactga gcgccgtagg cagtgatttt tatggtcaat cgctgctaac
     5941 gcaaaccaat caatctggtg tttatgtcga taaatgcctg attgtgccgg gagaaaatac
     6001 gtcgagttat ttatcattac tcgataatac cggtgaaatg ctggttgcta taaatgacat
     6061 gaatattagc aacgctatta cagctgaata tctcgcacag caccgtgaat ttattcagag
     6121 ggcaaaggtc attgtcgcgg actgtaatat cagtgaagag gcactggcat ggattctgga
     6181 taatgccgcc aacgtacccg tatttgtcga tccggtttca gcgtggaaat gtgtcaaagt
     6241 gcgcgaccgt ctaaatcaga tccacactct gaagccaaac cgccttgaag cggaaaccct
     6301 gagtgggatt gcgctgtcag ggcgtgacga tgtggcaaaa gttgctgcct ggttccatca
     6361 acatggcctg aaccgactgg tattgagcat gggcggcgac ggcgtttatt acagcgatat
     6421 cagcggtgaa aatggctggt ctgcgccgat caaaaccaat gttattaatg ttaccggagc
     6481 gggcgatgcc atgatggcgg gacttgcttc gtgttgggta gacggaatgc cgtttgccga
     6541 atctgttcgt ttcgcacagg gatgttcgtc aatggcgctc tcctgtgaat acaccaataa
     6601 ccccgattta tcgattgcca acgttatatc gttagtggag aacgcagaat gtctgaatta
     6661 aaaatttccc ctgaattatt acaaatttct ccggaagtgc aggacgcttt aaaaaacaaa
     6721 aaaccggttg tggcgctgga atcaaccatt atttctcacg ggatgccgtt cccacaaaat
     6781 gcccagaccg caattgaagt agaagaaact attcgtaaac agggcgcagt acctgccact
     6841 atcgccatta ttggcggcgt gatgaaagtg ggtttaagca aagaaaaaat tgaattactg
     6901 ggtcgtgaag ggcataacgt gaccaaagtt agtcgtcgcg atttaccttt tgttgttgcc
     6961 gccggaaaaa atggcgcaac cactgtggct tcaacgatga ttattgcggc gcttgccgga
     7021 attaaagtat ttgccaccgg gggaattggt ggtgtgcatc gcggggcgga acataccttc
     7081 gatatttctg ccgatttgca agaactggca aatactaatg tcaccgttgt ttgtgccggg
     7141 gcgaaatcta ttctcgattt aggattaacc actgagtatt tagaaacctt cggtgtgccg
     7201 ttaattggtt atcagactaa agcgctgcct gcgtttttct gccgtaccag ctcgtttgac
     7261 gtcagcattc gtctcgacag cgccagtgaa attgcccgtg caatggcggt gaaatggcaa
     7321 agcgggctga acggtggcct cgtggtagcg aacccgatcc cggaacagtt tgcgatgccg
     7381 gaggaatcta tcaatgcagc catagatcaa gccgtcgccg aagccgaaga gcagggcgtt
     7441 attggtaaag aaagtacacc gttcctgctg gcgcgcgttg ctgagctgac cggcggtgac
     7501 agcctgaaat ccaatatcca gctggtgttc aacaacgcca ttctggcgag tgaaattgcc
     7561 aaagaatacc agcgtctcgc gggttaattt ctgacgggca gaaagccgtc gcccgtactc
     7621 atcaagcata ccaaaacctg gcgctgacgc cgggtttact ggcatgaagg gaaaatcatt
     7681 atggatataa tgagaagtgt tgtggggatg gtggtgttac tggcaatagc atttctgttg
     7741 tcagtgaata aaaagagcat cagtttgcgc acggttggag ccgcactgct gctgcaaatc
     7801 gctattggtg gcatcatgct ctacttccca ccgggaaaat gggcagtaga acaggcggca
     7861 ttaggcgttc ataaagtgat gtcttacagt gatgccggta gcgccttcat ttttggttcg
     7921 ctggttgggc cgaaaatgga tgtcctgttt gacggtgcgg gttttatctt cgcctttcgc
     7981 gtacttccgg cgattatttt cgttactgcg ctcatcagtc tgctgtacta cattggcgtg
     8041 atggggctgc tgattcgcat ccttggcagc attttccaga aagccctcaa catcagcaaa
     8101 atcgaatctt ttgttgcggt taccactatt ttcctcgggc aaaatgagat cccggcgatc
     8161 gttaaaccgt ttatcgatcg cgtgaatcgc aacgagttgt ttaccgcaat ttgtagcggg
     8221 atggcgtcca ttgctggttc gatgatgatt ggttatgccg gaatgggcgt accaattgac
     8281 tacctgttag cggcatcgct gatggcgatc cccggcggta ttttgtttgc acgtattctt
     8341 agcccggcaa ccgagccttc gcaggtcaca tttgaaaatc tgtcgttcag cgaaacgccg
     8401 ccaaaaagct ttatcgaagc ggcggcgagc ggtgcgatga ccgggctgaa aatcgccgct
     8461 ggtgtagcga cggtggtaat ggcgtttgtc gcaattattg cgctgatcaa cggcattatc
     8521 ggcggaattg gcggctggtt tggtttcgcc aatgcctctc tggaaagtat ttttggctat
     8581 gtgctggcac cgctggcgtg gatcatgggt gtggactgga gtgatgccaa ccttgctggt
     8641 agcctgattg ggcagaaact ggcgattaac gaattcatcg cttacctgag tttctcccca
     8701 tacctgcaaa cgggcggcac gctggaagtg aaaaccgtgg cgattatatc ctttgcgctt
     8761 tgtggttttg ctaactttgg ttctatcggt gttgtcgttg gcgcattttc ggctatttcg
     8821 ccaaaacgtg ctccagaaat cgcccagctt ggtttacggg cgctggcagc agcaacgctt
     8881 tccaacctga tgagtgcgac tattgccggg ttctttattg gtctggcgta atgctacgat
     8941 ttgtagaccg gataaaacgt attaagcgtt gcatccggca atcgcgctat tgcaaataat
     9001 ttttactgca tcatcccgga gaatttattc tccgggtcca tctccagcgt cagtcctgac
     9061 aactgctttc tgtttttaat tagatatcct ttcttgcttt taattaataa accgtcgtga
     9121 atgaattgcg cgagaacata taaaagatgt cgataagaaa cgcctaaata ctctgccgct
     9181 tgcgtatgct tttcgtgata aagatcacct tcttgtgaga gtaatataaa tgctgccagg
     9241 cgattcatta acggaaatga ttgattctga gttaaagaaa caatattacg ataattttta
     9301 tgacttaagg tgacgcaaag ttttcgtaaa aatagggtgt cgtttaataa cagcggacgg
     9361 taatgtttca tagggagcgc aaggcaccaa cactcttcaa tagcctgcac cgcacgcggt
     9421 tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac agggagcagc aaagaaatcg
     9481 atcagcgaca cgcgaccatt agcaagcgtg gcgtaaagcc tggcgcggcc tcgcgtcaga
     9541 taaaacagcc atgacggctg ttgcccttcc tgcacaatgt agtcacgcgc taaaaaatga
     9601 aacagccgcg tatcggctga aacatccgtt gaaaagcaat ccttaaacgc agattcactc
     9661 atcaattgct gcttaaggtc attattgtgg atttctttca cgatgtttat ccgctatgag
     9721 atttctcata tttagcatac gtacgctgtt gttaagattc aatcatcagc ccggtcgaat
     9781 tttctttgct gtagaaaaaa ataacgcaac tggaaacaga ggaaataaac aatggaaaag
     9841 agaaaaatta ttctggattg tgagccgggt catgatgatg ctattgcaat gatgatggca
     9901 gcgaaacatc cggcaataga tttattaggc atcactattg tagcgggtaa tcagacgctt
     9961 gataaaacat taattaatgg cctgaatgtt tgccagaaac tggagattaa tgttccggtt
    10021 tatgcgggga tgccgcaacc cattatgcgt aaacaaatcg ttgccgataa tattcacggt
    10081 gaaaccggac tggatggccc ggtattcgag ccgctgaccc gccaggcaga aagtacccat
    10141 gcggtgaaat atatcatcga taccctgatg gcaagcgatg gcgatatcac tctggtgccg
    10201 gttggtccgc tttcaaatat cgcggtggca atgcgtatgc aacccgcaat cctgcccaaa
    10261 atccgtgaaa ttgtgctaat gggcggcgct tacggtacag gcaacttcac cccatctgcc
    10321 gagttcaata tctttgccga cccggaagcc gcccgcgtgg tgttcacctc cggcgttcca
    10381 ttagtgatga tgggcctcga tcttaccaac cagaccgttt gcaccccgga tgtaattgct
    10441 cggatggaaa gggtaggcgg ccctgccgga gagctgttca gcgacatcat gaacttcact
    10501 ctcaaaacgc agttcgaata ctacggcctg gctggcggcc cggtgcatga cgccacctgc
    10561 attggttatc tgatcaaccc tgatggcatt aaaacccagg atatgtacgt cgaagtggac
    10621 gtcaacagcg gcccttgcta cgggcgtacg gtctgcgacg aactgggcgt tcttggcaag
    10681 cccgctaata ccaaagtcgg catcactatt gataccgact ggttctgggg cttagtcgaa
    10741 gagtgcgtgc gcggctacat caaaacccat taagtgttga taacaagccg ggacagtatc
    10801 gcaagatgca ggcccggcta tttctgatgg gcagaaagcc gtcgcccaaa atgacatgaa
    10861 gagataatca ctatggatgt catgagaagt gttctgggaa tggtggtact gctggcaatt
    10921 gcgtttttac tgtcagtaaa caagaagaag atcagcctgc gtaccgttgg cgcggcgtta
    10981 gtgttacagg tcgtgattgg cgggattatg ctttggttac cgccagggcg ttgggtcgca
    11041 gaaaaagtcg cttttggcgt ccacaaagtg atggcgtaca gcgacgcagg tagcgcattt
    11101 atcttcggtt ctctggtcgg accgaaaatg gataccttat ttgatggcgc aggatttatc
    11161 tttggtttca gggtattacc ggcaattatc ttcgtcactg cactggtgag tattctctac
    11221 tacatcggtg tgatagggat cttaattcgc attctcggcg gtatcttcca gaaagcttta
    11281 aatatcagca agatcgagtc attcgtcgcg gtcaccacca ttttcctcgt gcaaaacgaa
    11341 attccggcga tcgtcaaacc ctttatcgat cgtctgaatc gcaatgaatt atttacagcg
    11401 atttgtagtg gcatggcctc gattgctggt tcgacaatga ttggttacgc cgcactgggc
    11461 gtacctgtgg aatatttgct ggcggcatcg ttaatggcga tccctggcgg gatcttgttt
    11521 gcccgcctgt taagcccggc tacggaatct tcgcaggttt cttttaataa cctctctttc
    11581 accgaaacac cgccaaaaag cattattgaa gccgatgcga caggggcaat gaccgggctg
    11641 aaaatcgccg ccggtgtagc gacagttgtt atggcatttg tcgccatcat tgcgttaatt
    11701 aacggtatta tcggcggcgt tggcggctgg tttggttttg aacatgcctc gctggagtcc
    11761 attttaggtt acctgctggc cccacttgcg tgggtaatgg gtgttgactg gagtgatgca
    11821 aatcttgccg ggagtttgat tggacagaaa ctggcaataa atgaatttgt cgcttatctc
    11881 aatttctcac cctatctgca aacggctggc actctggatg ctaaaaccgt ggcgattatt
    11941 tccttcgcgt tgtgcggttt cgctaacttt ggttctatcg gggtggtggt gggggcgttt
    12001 tctgcggttg cgccacaccg tgcgccggaa atcgcccagc ttggtttacg cgcgctggcg
    12061 gcggcgacac tttctaatct gatgagtgcc accattgccg ggttctttat tggtttagcg
    12121 tagtgtgcaa tttatgagcc ggattagatg cggtcaagcg tcgaatccgg caacattgtt
    12181 acaccattgg cactaatgac agcgcgttat cggcagacag ggtagggttg tttaagctgc
    12241 cgctggcgcg tgaaattgcc gcgcaggcca tcgcaaaacg ggcgctgtca cggaaattgt
    12301 ttccctccag aaagctatat accagacccg ccataaaacc atcgtcagca ccaaaactgt
    12361 ctaccgtcgt atgcgctggc acagtcagca aaaattgttc accatccttt tcgctgcaat
    12421 aaactgactc atcgggcaaa taaacagttg ctgaacacct tgctgatgca atgcattcac
    12481 tgcggcatta cggtcagcat cgctggtgat cgcctgtccc cataaaattt ccagctccgg
    12541 taaagtgggt ttcagggtgt gaatatgcgc cagccagtgt ttgattttgc ccgctttgaa
    12601 ttctgaaacg gtatcgacaa acaccgggat ctcatcagcg agggtaaaga cccattccag
    12661 cgcctcggct gtcaggttgc aatcaaccag taccacgccc gcatgacgaa gtaaatcgcg
    12721 cgacccgttc agtagttgcg gtgtcaactg ttccagcaga tgggtatcgt taatcgccag
    12781 cacggtttga tcgtctcgat tggcaattgc cagatacgtc gatgtgcttt gaccatgcaa
    12841 acgaacgcag ccggagacat tcacgccggc acggcgcgtt tcttccagga gcatttcgcc
    12901 ataaaagtca tcgccaatca ctgaaagcaa atggacgtca cggcctaaca gcgccagatt
    12961 gtgggcgata ttgcgtccaa cgccgcctgc cgaacaatga attgtaccgg gatgagaagc
    13021 cgcttgcggg taatggatat ccgccatccc gcgaatatcc atattgattg tccccaccac
    13081 tacgcagtat tcctgctcgg tgagaatgta acctttgcct ttaatccggc ctttgcgcat
    13141 taaatccata atatgcgccg caacgcgcga acgactgatt tgcagcatgt ccgcaatttc
    13201 gttctgctga atcagcgggt tacgccgtaa aattgcaagg atctcctttt cccggttatt
    13261 catcttcagg ctaccgcttt ttcagtttgt tgcgctttca gccaggcgat ctcttctgcc
    13321 cagatatccg ggttgatggt ttcaaggatc agcggaatgc cgtcgaaacg gtcgttctgc
    13381 ataatccagc ggaacgcatc atgaccgata ttgccttcac cgaggctatg atggcggtca
    13441 acgcggctgc caaaggtact tttcgcatcg ttaaggtgca tcccgcgcag atacttaaag
    13501 ccgacaatac gggcaaaatc agcgaacgtt ttttcgcatt cggctggagt acgcaaatca
    13561 tacccggcag cgaaagcatg gcaggtatca atgcagacgc cgacgcggga tttatcttcc
    13621 acgccgtcga taatcgccgc gagatgttcg aatttaaacc ctaagttact gccctgaccg
    13681 gcggtgtttt caatcaccgc tgttacacct tgagttttat ccagcgcaat gttgatggat
    13741 tcggcaatac gcgcaaggca atcctcttct gaaatctgca tcagatggct gccagggtgg
    13801 aagttgagca aagaaagccc cagttgttcg caacgctgca tttcatctat aaaggcatcg
    13861 cgcgattttt ccagagcttc agtgaccgga tgtccgaggt tgatcagata actgtcgtgg
    13921 ggaagaattt gcgccgatgt gtagtgatat ttttcacagg cggctttgaa ttcatcgatg
    13981 gtttgcgtcg tgagcggtgc ggcacgccac tgacgttggt ttttggtgaa caaggcaaac
    14041 gcggttgcgt cgatttcggc ggcgcgaatt gcggcatttg ccagaccgcc agcagcacta
    14101 acgtgcgctc caatgtattt catgcgagga ctcctgttaa acccgctgga ggaaaacggt
    14161 aatgatagcg ggttaacagg agcaagaagt agtggtttat gcgatgacgc tttgaatcac
    14221 atagttaatc gcaccaccac caacaatcag ccaggcaaac agtaccagtg ccatcagcag
    14281 aggtttcgcc ccagcttttt tcagcgcgct gacgtgagtg gtcagaccca gcgccgccat
    14341 cgccattgcc agcaggaagg tatccagcgt taccagcatg ttcaccacgc tctgcggtaa
    14401 caggtggaac gagttaaaga tggcaactac gatgaacaag atggcaaacc acggaatagt
    14461 gattttgctt ttctcgccgc tgttcgcccc agacagctgt ttaacacgcg ccgccagcag
    14521 gatgaggaac ggagccagca tcatcacgcg cagcattttg gaaataactg ctgcgttttc
    14581 tgcatccggg ctgatggcat gacctgccgc caccacctgc gccacttcgt gcacagtaga
    14641 accaatgtag ataccgaaag tttccggact aaaccattga gacatcagcg gatatatcgc
    14701 cgggtagagg aaaatcgcga cggtcccgaa gataacaacg gttgcaacag ccacggttac
    14761 tttactggct tccgctttca ctaccggctc agtcgccagt accgcggcag caccacagat
    14821 actgctaccg gcaccgatca accaactggt gtgcttatcc agaccaaaca ctttctgccc
    14881 caggaagcaa gccagcagga aggtactgga cagcgtcagc acgtcaatga tgatcccact
    14941 gataccgaca tcggcaattt gcgagaacgt cagacggaag ccataaagaa tgatacccag
    15001 acgtaataaa tattgcttgg caaacagcac accaccgtca cagcttttcc agatgtgcgg
    15061 atagatggtg ttgcctaaaa ccatccccaa caagattgcg agggtgaggg cactaaaccc
    15121 ggcacccgca accgcgggaa tggaaccacc ccacagggcg accccggtga taactgcact
    15181 cagggctaac cccggaataa aatgccacag tgtacgatgt tgtttctgta aggtgatatt
    15241 cgtcataacc ctctccttta accgggcata aggttacgac taactggttt aaaaataaaa
    15301 ttgattatat atttataatt aatctttata agtggtaagc gactatgcac atcaccctcc
    15361 ggcagttgga agtttttgca gaagtattga aaagtggatc aaccacccag gcgtcggtga
    15421 tgctggcgtt gtcgcaatca gcagtgagcg cagccttgac tgacctggaa gggcagcttg
    15481 gcgttcaact gtttgatcgc gtggggaaaa gactggttgt taatgaacac gggcggctgc
    15541 tctatccgcg agcgttggca ctgcttgaac aggcggttga aatcgaacaa ctgtttcgcg
    15601 aagacaacgg cgcgattcgt atctatgcca gtagtaccat cggtaactac attctgcctg
    15661 cagttatcgc ccgttatcgc catgattatc cgcagttgcc gattgaactt agcgttggga
    15721 acagccagga cgtgatgcaa gcggtgctgg atttccgcgt tgatattggc tttattgaag
    15781 gaccgtgcca cagcactgaa atcatttctg aaccgtggct ggaagacgag ctggtggttt
    15841 tcgccgcgcc gacttcgccg ttggctcgtg gtccggtcac cttagaacag ctggccgctg
    15901 cgccgtggat cctgcgtgaa cgcggttccg gcacgcggga gattgtcgat tatctgttgc
    15961 tgtcacattt accgaagttt gagatggcga tggaattagg taactccgag gcaatcaaac
    16021 atgcggtgcg tcatgggttg ggaattagtt gcctgtcgcg acgtgtgatt gaagatcaat
    16081 tgcaggcagg cacattaagt gaagttgcgg tccctctgcc gcgcctgatg cgtacgttgt
    16141 ggcgtataca tcatcggcaa aaacaccttt ccaacgcgct acggcgcttt ctggactatt
    16201 gcgatcccgc aaatgtgccg cgttaagttg ctgtacaaga acatgctggt gctgtgtcga
    16261 tttcgtgacg cagcgccttc agcatgcatt cgccagaaaa gagattggct gctttactta
    16321 taatccctgg gcgatcatga aggtgtctta taaccgtgta tttctgccgg aaggattgcc
    16381 aatcgtctgc tacaatcgcg ccatcatttt taagatggat agcatttttg tatgggttcc
    16441 gaaactaaaa ctacagaagc gccgggctta cgccgtgaat taaaggcgcg tcacctgacg
    16501 atgattgcca ttggcggttc catcggtaca ggtctttttg ttgcctctgg cgcaacgatt
    16561 tctcaggcag gtccgggcgg ggcattgctc tcgtatatgc tgattggcct gatggtttac
    16621 ttcctgatga ccagtctcgg tgaattggct gcatatatgc cggtttccgg ttcgtttgcc
    16681 acttacggtc agaactatgt tgaagaaggc tttggcttcg cgctgggctg gaactactgg
    16741 tacaactggg cggtgactat tgccgttgac ctggttgcag cacagctggt catgagctgg
    16801 tggttcccgg atacaccggg ctggatctgg agtgcgttgt tcctcggcgt tatcttcctg
    16861 ttgaactaca tctctgttcg cggctttggt gaagcggaat actggttctc actgatcaaa
    16921 gtcacgacag ttattgtctt tatcatcgtt ggcgtgctga tgattatcgg tatcttcaaa
    16981 ggcgcgcagc ctgcgggctg gagcaactgg acaatcggcg aagcgccgtt tgctggtggt
    17041 tttgcggcga tgatcggcgt agctatgatt gtcggcttct ctttccaggg aaccgagctg
    17101 atcggtattg ctgcaggcga gtccgaagat ccggcgaaaa acattccacg cgcggtacgt
    17161 caggtattct ggcgaatcct gttgttctat gtgttagcga tcctgattat cagcctgatt
    17221 attccgtata ccgatccgag cctgctgcgt aacgatgtta aagacatcag cgtcagtccg
    17281 ttcaccctgg tgttccagca cgcgggtctg ctctctgcgg cggcggtgat gaacgcagtt
    17341 attctgacgg cggtgctgtc agcaggtaac tccggtatgt atgcgtctac tcgtatgctg
    17401 tacaccctgg cgtgtgacgg taaagcgccg cgcattttcg ctaaattgtc gcgtggtggc
    17461 gtgccgcgta atgcgctgta tgcgacgacg gtgattgccg gtttgtgctt cctgacctcc
    17521 atgtttggca accagacggt atacctgtgg ctgctgaaca cctccgggat gacgggtttt
    17581 atcgcctggc tggggattgc cattagccac tatcgcttcc gtcgcggtta cgtattgcag
    17641 ggacacgaca ttaacgatct gccgtaccgt tcaggtttct tcccactggg gccgatcttc
    17701 gcattcattc tgtgtctgat tatcactttg ggccagaact acgaagcgtt cctgaaagat
    17761 accattgact ggggcggcgt agcggcaacg tatattggta tcccgctgtt cctgattatt
    17821 tggttcggct acaagctgat taaaggaact cacttcgtac gctacagcga aatgaagttc
    17881 ccgcagaacg ataagaaata agttttctcc cttccttgct aagccctctc aaccgagagg
    17941 gctttttcaa ttccatttcc ctgacaaatc atgcggatat aaaatttaac atttggattg
    18001 ataattgtta tcgtttgcat tatcgttacg acgcaatcaa aaaaggctga caaatcagag
    18061 gctgttccgg ctttctggga tgatcacctg catacaaata agtccaccgc gatgctgccg
    18121 tacgcaaggg gacgtaaaga agatgtgagc gatacccatt ttattttcgt agttacctca
    18181 tggagatatg gaatgtttag gttgaaccct ttcgtacggg tcgggctgtg tttgtccgct
    18241 atttcttgtg catggcctgt gttagcggtc gatgatgatg gcgaaacgat ggttgtcact
    18301 gcatcttccg tggaacaaaa tcttaaagat gcacccgcca gtatcagcgt cattacccag
    18361 gaagacctgc agcgaaaacc ggtacagaat ctgaaggatg tcctcaaaga agtgcctggc
    18421 gtacaactga cgaacgaagg ggataaccgt aagggcgtta gtattcgtgg tctggacagc
    18481 agctacaccc tgattcttgt cgacggtaaa cgcgttaact cccgcaatgc cgtcttccgc
    18541 cacaatgatt tcgatctgaa ctggatcccg gtcgattcca tcgaacgtat tgaagtggtg
    18601 cgtggcccga tgtcgtcgct atacggctcc gatgcgctcg gcggtgtagt gaatatcatc
    18661 accaaaaaaa tcggtcagaa atggtcgggt accgttaccg tcgataccac cattcaggaa
    18721 catcgcgatc gcggtgatac ctataacggt cagttcttta ccagtggacc attaattgat
    18781 ggtgtgctgg gaatgaaagc ttacggcagc ctggcaaaac gtgaaaagga tgacccgcaa
    18841 aactcaacga ccaccgatac cggagaaacg ccgcgtattg aaggattctc cagccgcgac
    18901 ggcaatgtcg aatttgcctg gacaccgaat caaaatcacg attttactgc cggatacggt
    18961 ttcgaccgtc aggatcgtga ttccgactcg ctggacaaaa accgcctgga acgccagaac
    19021 tactgcgtca gccataatgg gcgttgggat tacggcacca gcgaactgaa atcctacggt
    19081 gagaaagtcg agaacaaaaa ccctggcaac agcagcccga taacttccga aagcaatacg
    19141 gtcgacggca aatacacgtt gccgctgacg gcgattaatc agtttctcac ggttggcggt
    19201 gaatggcgtc acgacaaact tagcgatgcg gtgaacctga ccgggggaac cagctccaaa
    19261 acgtctgcca gccagtacgc gctgtttgtg gaagatgaat ggcggatctt cgagccgctg
    19321 gcgctgacga ccggcgtgcg tatggacgat cacgaaacct acggtgaaca ctggagtccg
    19381 cgtgcctacc tggtttataa cgccaccgac accgtaacgg tgaaaggggg ctgggcgacg
    19441 gcatttaaag caccttctct gttgcaactt agccctgact ggacgagcaa ttcctgccgt
    19501 ggcgcatgta agattgtggg tagcccggat ctgaaaccag aaaccagcga aagttgggag
    19561 ctggggcttt actacatggg tgaagaaggc tggctggaag ggggtgaatc cagcgttatc
    19621 gttttccgta acgatgtgaa agatcgtatc agcatcagcc gtacgtctga cgtcaacgct
    19681 gcaccgggct accaaaactt tgttggtttt gagacgggcg ctaacggacg gcgcataccg
    19741 gtatttagct actacagcgt taacaaagct cgtattcagg gcgtggaaac cgaactgaaa
    19801 attccgttca acgatgaatg gaaactgtcg atcaactaca cctacaacga tggtcgtgat
    19861 gtcagcaacg gcgaaaacaa accgctatcc gatctgccgt tccatactgc taacggtacg
    19921 ctggactgga aaccgctggc gctggaagac tggtcattct atgtttctgg tcactatacc
    19981 gggcagaaac gcgccgacag cgcgacggct aaaacaccgg gcggttatac catctggaat
    20041 accggcgcgg cctggcaggt gactaaagac gtcaaactgc gcgcaggcgt gctgaacctt
    20101 ggcgacaagg atctcagtcg tgacgactac agctataacg aagacggacg tcgttacttt
    20161 atggcagtgg attatcgctt ctgatgagaa gatgcccggc gaaccgggcg gactttcact
    20221 tcagtaaata ctgcgcatgg aagcgcaggt gatcctctat aaaagaggcg atgaagtagt
    20281 aactgtgatc atatcccggc tgaatacgca gcgtcatcgg ccacgctttc tggcgcgcgg
    20341 cttctgccaa taccgcaggt tgcaactggt cggcaagaaa ctgatcatta tcgccctgat
    20401 cgataagcgt cgggatcgca tcctgcgcgt tactggcata catcagcgcg caactatccc
    20461 attccagcca tgcatttttg tcctcaccta aataggtgct aaacgctttg attccccacg
    20521 ggacgctgca cggattcaca atgggcgcaa aggccgaaac gctggtgtat ttacccggat
    20581 ttttcagcgc cataatcagc gcaccgtgac cacccattga gtgaccgcta atggcgcagc
    20641 ggtcgctgac attaaattgc gactgaacca gcgccggtaa ttcatcgcgc agataatcat
    20701 acatccggta atacgtcgcc cacggcggtt gcgtggcatt aagataaaag cctgcgccct
    20761 ggcctaaatc gtagccatcg tcgttggcaa ccttttcgcc gcgcgggctg gtgtctggca
    20821 tcaccagtac aatccccagt tccgccgcta cccgctgggc acccgccttg gtggtgaagt
    20881 tctcgtcatt gcaggttaat ccggaaagcc agtacagcac tggtggcggg gtgtgatcac
    20941 gagggggtgg gagaaagata ctgaacgtca tcgggcagtt taaggtactg gagtcgtgtc
    21001 gccagcgttg ctgccagcct tcaaaacagc ggtgctcttc gagcatttcc atgcgtggct
    21061 ccttgttgtg ttgtttgcaa tttcttcatc ataccgttta ttcaatgtgc tgtgagtaac
    21121 tttcacttcc gtatttgcat aacgatgttt taacatctgc tgatgaaagg cagcggcaat
    21181 tacaataatt atcgctgtga atactggatt atgtgcgccg cctcacgcac aataatcaag
    21241 ctgtaaatca gcttaataac tttgccccca cgcagggcgg aggcgtcaca cctgcaggag
    21301 aaatcataaa tgccatcact cagtaaagaa gcggccctgg ttcatgaagc gttagttgcg
    21361 agaggactgg aaacaccgct gcgcccgccc gtgcatgaaa tggataacga aacgcgcaaa
    21421 agccttattg ctggtcatat gaccgaaatc atgcagctgc tgaatctcga cctagctgat
    21481 gacagtttga tggaaacgcc gcatcgcatc gctaaaatgt atgtcgatga aattttctcc
    21541 ggtctggatt acgccaattt cccgaaaatc accctcattg aaaacaaaat gaaggtcgat
    21601 gaaatggtca ccgtgcgcga tatcactctg accagtacct gtgaacacca ttttgttacc
    21661 atcgatggca aagcgacggt ggcttatatc ccgaaagatt cggtgatcgg tctgtcaaaa
    21721 attaaccgta tcgtgcagtt ctttgcccag cgtccgcagg tgcaggaacg tctgacgcag
    21781 caaattctta ttgcgctaca aacgctgctg ggcaccaata acgtggctgt ctcgatcgac
    21841 gcggtgcatt actgcgtgaa ggcgcgtggc atccgcgatg caaccagtgc cacgacaacg
    21901 acctctcttg gtggattgtt caaatccagt cagaatacgc gccacgagtt tctgcgcgct
    21961 gtgcgccatc acaactgatt aaaaggcagg aaccatggag cgcaacgtca cgctcgattt
    22021 tgttcgcggc gtcgccattc tggggatcct gctattaaac atcagcgcct ttgggctacc
    22081 aaaggcggct tatctcaatc ccgcctggta cgatgctatt acgccgcagg atgcatggac
    22141 ctgggcattt ctcgatctca tcggccaggt gaaattcctc acgctttttg cgctgctgtt
    22201 tggtgctggc ctgcaaatgt tgctgccccg tggcagacgc tggatccagt cacggttaac
    22261 gctgttagtc ttgttgggct ttattcacgg tttattgttc tgggacggcg atattctgct
    22321 ggcttacggg ctggtgggct taatctgctg gtggctggtg cgcgatgcgc catcggtaaa
    22381 aagcctgttt aataccggcg tcatgcttta tctggtgggg cttggcgttt tgctgttatt
    22441 ggggctgatt tccgacagcc agaccagccg cgcctggacg ccggatgcat cggctatttt
    22501 gtatgaaaaa tactggaagc ttcacggcgg cgttgaagcg atcagtaatc gtgccgatgg
    22561 tgttggcaac agtttactgg cactgggcgc acagtatggc tggcaactgg cagggatgat
    22621 gctcattggt gcggcgctga tgcgcagcgg ctggctgaaa gggcagttca gcttacgtca
    22681 ctatcgtcgt actggttttg tgctggtggc gattggggtg accattaacc ttcctgccat
    22741 cgccctgcaa tggcagctgg actgggcgta tcgctggtgt gccttcttac ttcaaatgcc
    22801 gcgggaactg agtgcgccgt ttcaggcgat tggctatgcg tcgctgtttt atggcttctg
    22861 gccgcaattg agccgcttta agctggtgct tgcgatcgcc tgcgtcggac ggatggcgct
    22921 gaccaactat ctattgcaaa cgctgatttg taccacgctt ttttaccacc tcggtttgtt
    22981 tatgcatttt gaccgcctgg agctgctggc gtttgttatt ccggtatggc tggcgaatat
    23041 cctcttctct gttatctggc tgcgttactt ccgccagggg ccggtggaat ggctctggcg
    23101 tcagttaact ttgcgtgctg ccggactggc aatatctaaa acatcaagat aacgatctgg
    23161 atcacattcg ttaacaaaac ggctgtaacc gtttccattg ctgtgactcg attcacgaag
    23221 tcctgtattc agtgctgaca aaatagccgc cagcaagcag tcatttactg caatctcata
    23281 acaggtagtg aatatgatca ccattcgtga tgtagcgcgt caggctggcg tctctgtggc
    23341 aacggtttcc cgggtgctca ataacagcac gctggtcagt gccgacacgc gtgaagcagt
    23401 aatgaaagcc gtgagtgagc tggattatcg gccaaacacc aatgcccagg cgctggcaac
    23461 tcaggttagc gacaccattg gcgtggtggt gatggacgtt tctgatgcgt ttttcggcgc
    23521 gctggtaaaa gcggtggatc tggtcgccca gcagcatcag aaatacgtgc taatcggcaa
    23581 tagctatcat gaagcggaaa aagagcgtca cgccattgag gtgttaattc gccagcgttg
    23641 taatgcgttg attgttcact caaaagcatt gagtgacgat gaactggcac aatttatgga
    23701 taacattccc ggtatggtgt taatcaaccg cgttgtgccg gggtacgccc atcgttgcgt
    23761 ttgcctagat aatctcagcg gtgcccgaat ggcgacgcgc atgttgctga ataacggtca
    23821 tcaacgtatt ggttatcttt cttccagcca cggcattgaa gatgacgcca tgcgtaaagc
    23881 aggctggatg agtgcgttga aagagcagga tattattccg ccggaaagct ggattggcac
    23941 tggtacgccg gacatgccgg gcggtgaggc ggcgatggtt gaactgctgg ggcgcaatct
    24001 acaacttacc gctgtatttg cttataacga caatatggcc gctggcgcac tgacagcatt
    24061 aaaagataat ggcattgcga ttccgttaca tctctcaatc atcggtttcg atgatattcc
    24121 catcgcccgt tacaccgacc cgcaattaac gaccgtgcgt tatcccattg cttcaatggc
    24181 taaattagcc accgaactgg ccttgcaggg ggcagcaggc aatattgatc ctcgtgccgc
    24241 cactgtttta tgccgacgtt agtgcgtcgc cattctgtcg caacgcgcca gaatgcggcg
    24301 gcgatcacta actcaacaaa tcaggcgatg taaccgcttt caatctgtga gtgatttcac
    24361 agtatcttaa caatgtgata gctatgattg caccgtttta atgttgtaac ccgtatgtaa
    24421 cagtgaataa tcactttcac cgaggtaata gcgtcataac aacaattaaa gccgttttct
    24481 ggagcgttac cgggcatgga agaacgaatt ttaaaaagtg agcttcggcg ttcagtaaca
    24541 cttcattaac tctactgccc cgccgagcat ttatctcaag cactaccctg cataagaaaa
    24601 accggagata ccatgaataa gaaggtgtta accctgtctg ctgtgatggc cagcatgtta
    24661 ttcggtgccg ctgcacacgc tgctgatact cgcattggtg taacaatcta taagtacgac
    24721 gataacttta tgtctgtagt gcgcaaggct attgagcaag atgcgaaagc cgcgccagat
    24781 gttcagctgc tgatgaatga ttctcagaat gaccagtcca agcagaacga tcagatcgac
    24841 gtattgctgg cgaaaggggt gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg
    24901 ggtacggtga ttgagaaagc gcgtgggcaa aacgtgccgg tggttttctt caacaaagaa
    24961 ccgtctcgta aggcgctgga tagctacgac aaagcctact acgttggcac tgactccaaa
    25021 gagtccggca ttattcaggg cgatttgatt gctaaacact gggcggcgaa tcagggttgg
    25081 gatctgaaca aagacggtca gattcagttc gtactgctga aaggtgaacc gggccatccg
    25141 gatgcagaag cacgtaccac ttacgtgatt aaagaattga acgataaagg catcaaaact
    25201 gaacagttac agttagatac cgcaatgtgg gacaccgctc aggcgaaaga taagatggac
    25261 gcctggctgt ctggcccgaa cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg
    25321 atggcaatgg gcgcggtaga agcactgaaa gcacacaaca agtccagcat tccggtgttt
    25381 ggcgtcgatg cgctgccaga agcgctggcg ctggtgaaat ccggtgcact ggcgggcaca
    25441 gtactgaacg atgctaacaa ccaggcgaaa gcgacctttg atctggcgaa aaacctggcc
    25501 gatggcaaag gtgcggctga tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta
    25561 ccttatgttg gcgtagataa agacaacttg gctgaattca gcaagaaata agactgattg
    25621 ggcgcagtcg tttactgcgc ccttattgta tggccaacaa ggtaaaatta tatggtcagc
    25681 tcaacgactc cgtcttccgg ggaatacttg ttggaaatga gcggtatcaa caagtccttt
    25741 cctggtgtta aggcacttga taacgttaat ttaaaagtcc ggccacattc tatccatgca
    25801 ttaatggggg aaaacggtgc aggaaaatcg acattattaa aatgcctgtt tggtatttat
    25861 caaaaagact ccggcaccat tttattccag ggtaaagaga tcgatttcca ttccgccaaa
    25921 gaggcactgg aaaatggtat ttcgatggta caccaggagt taaacctggt attacaacgt
    25981 tcggtgatgg ataacatgtg gctggggcga tatcccacca aaggcatgtt tgtcgatcag
    26041 gacaaaatgt accgcgaaac caaagcgatt tttgatgaac tggatattga tatcgatccg
    26101 cgtgcgcgcg tcggcacatt atccgtttcg caaatgcaga tgatcgaaat cgccaaagcg
    26161 ttttcctata acgcgaaaat tgtgattatg gatgaaccga cttcttcgtt aaccgaaaaa
    26221 gaggtcaatc atctgttcac tattattcgt aaattaaaag agcgcggctg cggtattgtt
    26281 tatatctcgc ataaaatgga agaaatcttc cagttatgtg atgaagttac cgtattgcgc
    26341 gacggtcagt ggatcgccac cgaaccgctg gcaggactga cgatggacaa gatcatcggc
    26401 atgatggttg ggcgttctct taatcagcgt tttcctgaca aagaaaacaa gccgggcgaa
    26461 gtcatcctcg aggtgcgtaa cctaacgtca ctgcgccagc cgtcgattcg cgatgtctcg
    26521 tttgatctgc ataaagggga gatcctcggt attgccgggc tggtgggggc gaaacgtacc
    26581 gatattgttg agacgttatt tggtattcgc gagaaatcgg ctggcaccat tacgttgcac
    26641 ggcaaacaga tcaataacca taatgccaac gaagccataa accacggatt tgcactggta
    26701 actgaggagc gccgctcaac gggaatttat gcctatctgg atattggttt taactcgtta
    26761 atttccaata ttcgcaacta caaaaataaa gttggtttac tggataactc gcggatgaaa
    26821 agcgataccc agtgggtgat tgattcgatg cgggtaaaaa cgccgggtca tcggacgcaa
    26881 attggttcgc tctccggtgg taatcagcaa aaggtgatta ttggtcgctg gctactaacg
    26941 caaccagaaa tattaatgct cgatgaaccg acgcgcggta ttgatgtcgg ggcgaagttt
    27001 gaaatttatc agttaattgc cgaactggcg aagaaaggca aggggattat tattatctcc
    27061 tctgaaatgc ctgagttgtt agggataaca gaccgtattc tggtcatgag caatggtctc
    27121 gtttccggaa ttgtcgatac aaaaacaaca acgcaaaacg aaattctgcg tcttgcgtct
    27181 ttgcaccttt aagattaggg gcttcccatg agtgcgttaa ataagaaaag ttttcttact
    27241 tacctgaaag agggcggtat ttacgtcgtt cttttagttt tgctggcgat tattattttc
    27301 caggacccaa catttttaag tctgttgaac ttaagtaata ttctcaccca gtcatcggtg
    27361 cgtattatta tcgcgctcgg tgtggcaggg ttaattgtca cccaggggac cgatctttct
    27421 gctggtcgtc aggtagggct ggcggcagtg gtggctgcga cattattgca gtccatggat
    27481 aacgccaaca aagtgttccc ggaaatggcg acgatgccga ttgcgctggt tattctgatt
    27541 gtctgtgcca ttggtgcggt gatcggtttg atcaacggtc tgattatcgc ttatctcaac
    27601 gtgacgccgt tcattaccac gctcggcacg atgatcatcg tctatggcat caactcgctc
    27661 tattacgact ttgtcggggc gtcgccaatt tctggttttg acagtggctt ctctaccttt
    27721 gctcagggct ttgtcgcgct ggggagtttc cgtctctctt acatcacctt ctacgcgttg
    27781 attgcggtgg cgttcgtctg ggtgttgtgg aacaaaaccc gcttcggtaa gaacattttt
    27841 gccattggcg gtaacccgga agcggcaaaa gtatctggtg tcaacgtcgg cctgaacctg
    27901 ctgatgatct acgcgttgtc tggcgtgttc tatgcctttg gcgggatgtt agaagccgga
    27961 cgtatcggct ctgccaccaa caacctcggc tttatgtatg agctggatgc tatcgcggcg
    28021 tgcgtggtag gcggtgtatc gttcagcggc ggtgtgggga cggtgattgg cgtggtgacc
    28081 ggggtaatta tttttaccgt catcaactat ggcctgacgt atatcggcgt aaacccatac
    28141 tggcagtaca tcatcaaagg ggcgattatt atcttcgccg tagcgctgga ttcactgaaa
    28201 tacgcgcgta agaagtgatg tgattgcgga tattgccgga tgcgtctctg gttttacaac
    28261 gttaccgggt gttctttctc gcctttctta aacttcactt ccccgagctc aatacaaccc
    28321 accgggcaga cgtgaccaca aagcagacaa cccacacatt tctcggtatt acaatgcggt
    28381 gtgcgggttt tctcgctcca ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa
    28441 cagcgtccac agccaacaca tttgtcaaga ttgatacggg gatagacaat ataactgcgg
    28501 tctaaatctt ccgccgggac aatattgtta ttcgccagac ctaccatctc ctgcaacgaa
    28561 tcaaatccct gatcggcgag ataatgcgat aacccgctcg ccatatcttc tacgatgcga
    28621 tagccgtact gcataatgcc ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac
    28681 tccgccgcat cttcccaggt ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt
    28741 tctggatggg tgcgcatttg ctgaatgaag cgcagggcga tcggttttac cgctttaccg
    28801 gaatatccgg aaatactcga tttaccgtta acgatcggca taccgatttt ctgattaaga
    28861 tcgatattgg tgatggattt aacggtgtta attgccgcaa tgccatctgc gccgccgcgc
    28921 ttcgccgcca gcgccacttc gcacatatca ccgatattcg gcgtcatctt cgccagcatt
    28981 ggcagcgtgg aaccccgttt cactgcccga caatattttt ttaccagctc cgggctttgc
    29041 ccgacatcgc tacccatcgc atgagaagtc atttgcggac aggagaagtt acactcgatc
    29101 atatccgcgc cagcttcttg caccaggcgc gccagctcct cccattgctg ctcattttcc
    29161 cccatgatcg aagcgatcaa tactttgtcc gggtaatctt ccttcagccg acgcagggcg
    29221 gtcagattct cttccaacgg atgttcagca atctgctcca tatttttgaa gccgataaaa
    29281 ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga cttcgttggc gataaaaaag
    29341 ccgatcgttt taaacaccac accgccccaa cctgtgtcgt aggctttggc acacatctca
    29401 tagcagttgc ctaccggcga agaagagagg cagaacgggt tgggaaagtt cacgccgcaa
    29461 aaagtaatcg aaagatcttt cgttaacatg agcaagctcc ctctaatata gtgatgaatc
    29521 gcctcggcgg cttctttccc ggttttcacg gcatagacca cggttttgtc accctcaaca
    29581 atatcgccag cagcaaagac ttgcggatcg cgggtctgat aattttgtgt tttgatggtg
    29641 ttacgctgct gctctaactc cgcaaaggca tccagtctgg catgctgacc gacggcgaga
    29701 ataattttat ctgccgccat cgtcagttcg cctgataacc gtacatgctt aaacgtgact
    29761 ttattgcctt ccacggctac tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg
    29821 gcgctggcaa actctttttc gcttgccgga aactcatcta actcttcacg tgctacgcaa
    29881 gttaccgcct gacagccgag aactttcagc gtgctggcta cgtccatcgc gacatcaccg
    29941 ccgccgataa ttaatgcgct ttgtggaatg ctgatatcgc cttgcgcctg tcgtacacgt
    30001 tgcaggaagt cgacggcaat ctcaacgtcg ctatgctcaa acagcggtag cccggaaccg
    30061 cttgataacc ccacggtgac cagtaccgcg cggttttccg ctttcagttg ctccagggtg
    30121 agtgttttac cgatttcgtt attgcactta atggtcacgc ccattttttc aatacgggcg
    30181 atctctgcat ccagcactga ttgcggtaac cggaattgcg gaataccgtt acgcaaccag
    30241 ccaccggggt gcgcttcttt ctcataaatc gtgacgtcat aaccctggtt tgtcagtgtc
    30301 acactggcct gcaatccggc aggacctgcg ccaataatcg cgactttgcc gagcgtttta
    30361 gtaccgggct gataaatttc catcccggtt tgttgttcaa aatcagtaac aaaacgctgt
    30421 aagcggccga tatcaatggg agcatcaaca ccggcacggg tacaaccgct ttggcataat
    30481 ttctccgtcg ggcacactct ggcacaaacg gcaccgaggg cattattttc gcgaattgtc
    30541 tcggcagcgc ctttaaaatt acgaaagtag attgagcgaa taaatttccc cggatcggtc
    30601 tgtgccgggc aagcctgact acagggagcg tcgtgacata ataaacagcg agaggcttct
    30661 ttaatcgcca gtaacggcgt aaaagccgga gtgagttcat ccagataatt ttgttgcggc
    30721 atgtgatctt ccttaatgct atgagtaatt atggcgaacg ctaaataaat attgcgtgct
    30781 gatattaagg ctatgcgcgt ggggaaattt aaactgtgaa aggattcaca aatagctgcg
    30841 gttcagaatg ttatatccgc agcttattga gtgaagtaat aattattctc aataattaac
    30901 agaaaagaga aaatattaac gatagaaaat aagataaaca atcatgccga tagataaaat
    30961 tccgaagcca atatatgcca ccatataagc gaagaagagt ccgggatttt ttgctcgcca
    31021 gacatacacc ggttccggtg catttttaac ccgtttatca atcgcgcctg cgcgccagcc
    31081 tttccatgct tcgcggaaac aaaatatcgg gataaggaaa aaaacggcaa cgacaaaaaa
    31141 ctgctgtaca tccataacta ctttccttgt ttcttttgta attcaagtaa ctgttcgggt
    31201 gtaacggcgg gatacccctg cgcatcttcc ggcacctggt gcatagccgg aatagggacc
    31261 agcggcccta aaaaacgcgg ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa
    31321 cgggcggcaa attcgcgaat acgtactgac agcatatctt tcggtgacgg tacggcataa
    31381 cactgagcct gaatccccat atgcagcgca ataaataatg ctcgctcaca gtggaaacgt
    31441 tgggtgataa taatgaaatc attagtatcg aaaactttgc gtgtacgcac gatggaatcc
    31501 agcgtacgaa agcctgcgta atcgagaaca atatctgatg ggtcgacacc agcagcgatt
    31561 aaatctttgc gcatggtcat cggctcgtta taactttgca atgcgttatc gccgctcagt
    31621 aatagataat ttaccttacc gctgttatag gcattaatcg ctccttgaat gcggtagcga
    31681 taatactgat taataacgcc ggtacgataa tattttgctg tgccgagcac cacgccgacc
    31741 tggcggtagg ggagatcctg caattcgtcg tagatataag gcgcggtttt ccagctcatc
    31801 cagcgatcga ggccgagcac agtcaacagc agcaagccga tcaggactaa caggctgagg
    31861 aacacgcgct ttaacatgca ggagactcga gctaaggaaa atttccatca ggctacttta
    31921 cccgctggtt ttgcgcaaga aacggcaaac tgtttgccgg gaatttcgac accaggagag
    31981 ggctggcaag ccggacaagg cgttcacgcc gcatccggca ccaggcttaa gcgagaagca
    32041 cgcggtcgat actgtgacag ccgagagctt tcagcgttgc ggaggtggca tcccactgaa
    32101 tcaacggcgc atcggctttt tctgccagaa ccgcgcgctg gatatccggg taatcataac
    32161 ccttgagatt caacagaatt aacgccccct gcaacggcgg cagagtcggg ttgaatgcgg
    32221 cgttttcagc gtagctgccg ctgaaaatac gaccgtcttt acattccagc gcgacaccgc
    32281 ttggtgactt actgtaaggc atgtgcgaac ggtttgccgc tgcaatcgcc gcctgagaaa
    32341 gcgcatcacc agtcagcgca tagccgtgat cctgctcgtc catcagcagc gttttaatct
    32401 ccagatcttt cggcccaaag gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc
    32461 ccggcaaatg aatacgcaga tccagaccgc tgttcagttc attcataaac tgacggcagt
    32521 gaccacaagg cgtatagtta acggtgatgg ctgcaagcgc tttttcacca ctcaaccagg
    32581 cgtggctgat cgcgctttgt tcggcatgaa cggtttgctg cattgttgca ccaataaatt
    32641 ccatattggc accaaaatac caggttccgc tcacaccgcg cgcaattgcg ccaacattaa
    32701 aattcgacaa tggcgtacgc gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg
    32761 cgtcttcgtc cagccccgtt gcgctcttca gcgatgagac ttgctccccg gtcaacaaag
    32821 cggggaagta cttgtctgcc agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt
    32881 gggcaaaagc ggtttgaaaa cgtggatgca tggcgttgcc tcataatcat gtaatgggat
    32941 aacagtttac gaacccgtta tggctttata tgtgatctga atctcattaa ttatgcaaca
    33001 cgtgaaactt acatcaaaaa tgcgcgacgt atcgcaaatt ttagcccatt actgccagaa
    33061 taatcgggaa aaggaacggt gcgatcagcg aagtaattat cccgcataac accagcgcta
    33121 gcgaactaaa tgcaccttcc tgataatcca gctcggcgca gcgcgccgta ccgagggcgt
    33181 gcgaggcagt ccccattgcc agaccgcgcg cagctttggt acgaatacgc atcgcattaa
    33241 gcaatgtatg gccaaatacc gcgccgagga tgccgacgaa aatcacgcaa actgcgctga
    33301 ttgccggaat accgccaata ctgccgccaa ccgacattgc aataggcgtg gtgactgatt
    33361 tcggcaggat tgacgcggtg atttccggtg aagcgcccat caacaatgcc acggaagtcc
    33421 ccgtcaccat cgcaaccacg ctgccgataa aacagatggt gatgatcgat ttccagcgcg
    33481 cgcggatctg gtgtagctgc tcatataaag gataggccag cgcgacgacc gccggttgca
    33541 gcagatcgtt tagcacttcg ctacctttaa agtaactgtc gtaagagatg ccggtcagca
    33601 ttaaaaaagg aatgatcacc accattgcta ccagcaacgg gttaagcagg ggaaacttat
    33661 accgtgccgc cagtttgcgg gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc
    33721 agatattcgc catcattctt ctgatccttt ctgacctacg actttacgtt caccgtgtac
    33781 cagttgtgaa ctccagctca ccaccagaaa aaccaccagc gtactgacag cacaggaaac
    33841 cactaccggg ccaaactgcg cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat
    33901 cggcacaaac aatagcgcca tatagcgaat cagtacgtag caccccggat tgacccattt
    33961 cgccggaaga atttgcaagg ccagcaggac aaacaggatc agcatcccga tgatgctgcc
    34021 cggaatggtt accggtagca gggaagcaat gaaaatgcct gcatacaggc aggcataaat
    34081 caggacgaaa gcgcgtaaat attgccagat aatgttcagt gtcttactca taatcaacac
    34141 cccagatgag atggattcat catacaatta attcgtaaaa cgtgctaccg atcacattaa
    34201 gaaatcagcg cttatttttt cgctacccgg tattacgcaa tgagcagcga actcaggcat
    34261 acatcaagca tagagaggga ttgcagtttg cagcaatccc tctttgcaga cctttaatcc
    34321 gctgaccata ggcgctaaca tagcgcctca tttttttgcg ggtgatgata tacgtgtgtt
    34381 actggcaccg atggagggag tgcttgactc tctggtgcgt gaattgctga ccgaagttaa
    34441 cgactacgat ctgtgcatca ccgagtttgt ccgcgtggtg gatcaactgc tgccggtaaa
    34501 agtctttcat cgcatttgcc ctgagctaca aaacgccagc cggacaccat ctggtacgct
    34561 ggtgcgcgtg cagttgttag gtcagttccc acaatggctg gcagagaacg ccgcccgtgc
    34621 ggtggagtta ggttcctggg gcgtggatct caattgcggc tgcccgtcga aaacggttaa
    34681 cggtagcggc ggcggggcga cgttactcaa agatcctgaa ctcatctacc agggggcaaa
    34741 agcgatgcgt gaagctgtac cggcgcattt gcctgtcagc gtgaaagtgc gtctgggctg
    34801 ggacagcggt gagaagaaat ttgaaatcgc cgatgcggtt caacaggctg gcgctacgga
    34861 gctggtggtg catgggcgga cgaaagagca gggttaccgc gcggagcata ttgactggca
    34921 ggcgattggc gagattcgcc agcggctgaa tattccggtg attgccaacg gtgaaatctg
    34981 ggactggcag agcgcgcaac aatgcatggc gatcagcggc tgcgacgcag tgatgattgg
    35041 tcgcggggcg ctcaatattc ccaacctgag ccgggtggta aaatataacg aaccgcgaat
    35101 gccgtggccg gaggtggttg ctttgctgca aaaatatacc cgtctggaaa agcagggcga
    35161 taccgggtta tatcacgttg cgcggattaa acagtggttg agttatttgc gtaaagaata
    35221 cgatgaagca acggaattat ttcagcatgt tcg
//
LOCUS       DADLZM010000028        35233 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLZM010000028 DADLZM010000000
VERSION     DADLZM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.028"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            116..658
                     /locus_tag="JNY73_001328"
     CDS             116..658
                     /locus_tag="JNY73_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8047397.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            739..1413
                     /locus_tag="JNY73_001329"
     CDS             739..1413
                     /locus_tag="JNY73_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8047398.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1429..3909
                     /locus_tag="JNY73_001330"
     CDS             1429..3909
                     /locus_tag="JNY73_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8047399.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPLVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3925..4958
                     /locus_tag="JNY73_001331"
                     /pseudo
     CDS             3925..4958
                     /locus_tag="JNY73_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5040..5378)
                     /gene="rcnB"
                     /locus_tag="JNY73_001332"
     CDS             complement(5040..5378)
                     /gene="rcnB"
                     /locus_tag="JNY73_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY8047400.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5597..6445)
                     /locus_tag="JNY73_001333"
     CDS             complement(5597..6445)
                     /locus_tag="JNY73_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY8047401.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            6566..6838
                     /gene="rcnR"
                     /locus_tag="JNY73_001334"
     CDS             6566..6838
                     /gene="rcnR"
                     /locus_tag="JNY73_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY8047402.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6930..7026
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7061..7849
                     /gene="thiM"
                     /locus_tag="JNY73_001335"
     CDS             7061..7849
                     /gene="thiM"
                     /locus_tag="JNY73_001335"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY8047403.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7846..8646
                     /gene="thiD"
                     /locus_tag="JNY73_001336"
     CDS             7846..8646
                     /gene="thiD"
                     /locus_tag="JNY73_001336"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY8047404.1"
                     /translation="MKRINALTIAGTDPSGGAGILADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8711..9529
                     /locus_tag="JNY73_001337"
     CDS             8711..9529
                     /locus_tag="JNY73_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY8047405.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            9581..10327
                     /locus_tag="JNY73_001338"
     CDS             9581..10327
                     /locus_tag="JNY73_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8047406.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10301..11266)
                     /locus_tag="JNY73_001339"
     CDS             complement(10301..11266)
                     /locus_tag="JNY73_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8047407.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11263..12266)
                     /locus_tag="JNY73_001340"
                     /pseudo
     CDS             complement(11263..12266)
                     /locus_tag="JNY73_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(12263..12739)
                     /locus_tag="JNY73_001341"
     CDS             complement(12263..12739)
                     /locus_tag="JNY73_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8047408.1"
                     /translation="MLARSSFHRKGIQKVLLCGIVIAYLTLPVNFTYALLFLGILLHG
                     VSYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQE
                     PVNGLTFNWSGMWTFGAVMIAIIAVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            12893..13945
                     /gene="fbaB"
                     /locus_tag="JNY73_001342"
     CDS             12893..13945
                     /gene="fbaB"
                     /locus_tag="JNY73_001342"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY8047409.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            14253..15107
                     /gene="gatY"
                     /locus_tag="JNY73_001343"
     CDS             14253..15107
                     /gene="gatY"
                     /locus_tag="JNY73_001343"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY8047410.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRLKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNLAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            15136..16398
                     /gene="gatZ"
                     /locus_tag="JNY73_001344"
     CDS             15136..16398
                     /gene="gatZ"
                     /locus_tag="JNY73_001344"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY8047411.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAVVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            16408..16860
                     /gene="gatA"
                     /locus_tag="JNY73_001345"
     CDS             16408..16860
                     /gene="gatA"
                     /locus_tag="JNY73_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY8047412.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16891..17175
                     /gene="gatB"
                     /locus_tag="JNY73_001346"
     CDS             16891..17175
                     /gene="gatB"
                     /locus_tag="JNY73_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY8047413.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQYRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            17179..18535
                     /locus_tag="JNY73_001347"
                     /pseudo
     CDS             17179..18535
                     /locus_tag="JNY73_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            18583..19623
                     /gene="gatD"
                     /locus_tag="JNY73_001348"
     CDS             18583..19623
                     /gene="gatD"
                     /locus_tag="JNY73_001348"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY8047414.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            19723..20502
                     /locus_tag="JNY73_001349"
     CDS             19723..20502
                     /locus_tag="JNY73_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8047415.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQEAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(20584..21483)
                     /gene="yegS"
                     /locus_tag="JNY73_001350"
     CDS             complement(20584..21483)
                     /gene="yegS"
                     /locus_tag="JNY73_001350"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY8047416.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            21889..22206
                     /locus_tag="JNY73_001351"
     CDS             21889..22206
                     /locus_tag="JNY73_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047417.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(22548..23909)
                     /locus_tag="JNY73_001352"
     CDS             complement(22548..23909)
                     /locus_tag="JNY73_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY8047418.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24056..24378)
                     /locus_tag="JNY73_001353"
                     /pseudo
     CDS             complement(24056..24378)
                     /locus_tag="JNY73_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(24569..25291)
                     /gene="baeR"
                     /locus_tag="JNY73_001354"
     CDS             complement(24569..25291)
                     /gene="baeR"
                     /locus_tag="JNY73_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY8047419.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHESGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(25288..26691)
                     /gene="baeS"
                     /locus_tag="JNY73_001355"
     CDS             complement(25288..26691)
                     /gene="baeS"
                     /locus_tag="JNY73_001355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY8047420.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(26688..28103)
                     /locus_tag="JNY73_001356"
     CDS             complement(26688..28103)
                     /locus_tag="JNY73_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8047421.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(28104..31169)
                     /gene="mdtC"
                     /locus_tag="JNY73_001357"
                     /pseudo
     CDS             complement(28104..31169)
                     /gene="mdtC"
                     /locus_tag="JNY73_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
     gene            complement(31170..34292)
                     /gene="mdtB"
                     /locus_tag="JNY73_001358"
     CDS             complement(31170..34292)
                     /gene="mdtB"
                     /locus_tag="JNY73_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY8047422.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPSASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            complement(34292..>35233)
                     /locus_tag="JNY73_001359"
     CDS             complement(34292..>35233)
                     /locus_tag="JNY73_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HAY8047423.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
BASE COUNT         8856 a         8628 c         9163 g         8586 t
ORIGIN      
        1 ttaaatcaat aaaacaatat gtttacattg cggcagcttt tttgatgtga gaaatttgtt
       61 tccaagagat acagactctt aacaaccaaa aataaatttt attaaggaac atattatgaa
      121 acgttcaatt attgctgccg ctgtcttttc ttcttttttt atgagcgctg gagtatttgc
      181 tgcagacgtt gataccggaa cattaaccat taaaggaaat attgcagaat ctccgtgtaa
      241 attcgaagcg ggtggtgatt cagtaagtat taatatgccg actgtaccaa ccactgtctt
      301 tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc ggtgtaacca gcagcatgtt
      361 aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc tcgttgatca caaacgacaa
      421 aataaccggt aacgataagg cgatagccag tagcaacgat accgtgggtt actatctcta
      481 tttaggtgat aacagcgatg tcctagatgt ttctgcacct tttaacattg agagttataa
      541 aacagcagaa ggtcaatatg ctattccgtt taaagcaaaa tacctgaaac tgacagataa
      601 ctcagtgcaa tcaggtgatg tgttatcttc tctggttatg cgtgtagcgc aggattaata
      661 aataaaattg caagtttctc caggtaataa acatggccgc tatccccatg gcggcttttt
      721 aatttaagag gcattaagat gaaaggatta ttatctttac tcattttttc tatggtcctt
      781 cctgcacatg ccggaattgt tatctacggg acgcgcatta tttacccggc agaaaataaa
      841 gaagttatgg tgcagttgat gaaccaggga aatcgttctt cgctgctgca ggcgtggatt
      901 gatgatggcg atacgtcgtt accaccagaa aaaattcagg ttcctttcat gttaacgcca
      961 ccagtggcaa aaataggggc aaattccggg cagcaagtaa aaatcaaaat tatgccgaat
     1021 aaactgccca ctaataaaga aagcattttt tatctgaatg ttctggatat tccaccaaat
     1081 agtccagagc aagaaggcaa gaatgcactg aagtttgcga tgcaaaacag aattaagttg
     1141 ttttaccggc cagcgggtat tgctccggta aataaagcga cctttaaaaa gttactggta
     1201 aatcgcagtg gcaatggatt ggtgataaaa aatgactcag ctaattgggt gacgatttcg
     1261 gatgtcaaag ctaataatgt taaagtcaat tatgaaacta ttatgattgc ccccttagaa
     1321 agtcagagtg ttaatatcaa aagtaataat gcaaataact ggtatctgac cattatcaat
     1381 gaccacggca actatattag tgacaaaatt taattgcagg agctgcctat gttgagaatg
     1441 accccacttg catcagcaat cgtagcgtta ttgctcggca ttgaagctta tgcagctgaa
     1501 gaaacctttg atacccattt tatgataggt ggaatgaaag accagcaggt tgcaaatatt
     1561 cgtcttgatg ataatcaacc cttaccgggg cagtatgaca tcgatattta tgtcaataag
     1621 caatggcgcg ggaaatatga gattattgtt aaagacaacc cgcaagaaac atgtttatca
     1681 agagaagtta tcaagcggtt aggcattaat agcgataact tcgccagcgg taagcaatgt
     1741 ttaacatttg agcaacttgt tcagggtggg agctatagct gggatatcgg ggtttttcgt
     1801 ctcgatttca gtgtcccgca ggcttgggtg gaagaactgg aaagtggcta tgttccaccg
     1861 gaaaactggg agcggggtat taatgcgttt tatacttctt attatgtgag tcagtattac
     1921 agcgactata aagcgtcggg taacaacaag agtacatatg tacgttttaa cagcgggtta
     1981 aatttactgg agtggcaact gcattctgat gccagtttca gtaaaacaaa taacaatcca
     2041 ggggtgtgga aaagcaatac cctgtatctg gaacgtggat ttgcccaatt tctcggcacg
     2101 cttcgcgtgg gtgatatgta cacatcaagc gatatttttg attctgttcg cttcagcggt
     2161 gtgcggttgt ttcgtgatat gcagatgttg cctaactcga aacaaaattt tacgccacgg
     2221 gtgcagggga ttgctcagag taacgcgctg gtaactattg aacagaatgg ttttgtggtt
     2281 tatcagaaag aggttcctcc tggcccgttt gcgattacag atttgcagtt ggccggtggt
     2341 ggagcagatc ttgatgttag cgtgaaagag gcggacggct cggtaaccac ctatctggtg
     2401 ccttatgcag cggtgccaaa tatgctgcaa cccggcgtgt cgaaatatga ttttgcggcg
     2461 ggccgtagcc atattgaagg ggcgagcaag caaagtgatt ttgtccaggc aggttatcag
     2521 tatggtttta ataacttatt gacgctgtat ggcggcacga tggttgctaa taattactat
     2581 gcgtttaccc tcggaacggg ttggaacacg cgcattggcg caatttccgt cgatgccacg
     2641 aagtcgcata gtaaacaaga caacggcgat gtgtttgacg ggcaaagtta tcaaattgcc
     2701 tacaacaaat ttgtgagcca aacgtcgacg cgttttggtc tggcggcctg gcgttattcg
     2761 tcgcgtgatt accggacatt taacgatcac gtttgggcaa acaataaaga taattatcgc
     2821 cgtgatgaaa acgatatcta tgacattgcc gattattacc agaacgattt tggccgtaaa
     2881 aatagctttt ctgccaatat gagccagtca ttgccagaag gctggggttc tgtgtcctta
     2941 agtacgttat ggcgagatta ctgggggcgt agcggcagca gtaaggatta tcagttgagt
     3001 tattccaata actggcggcg gataagctat accctcgcgg caagccaggc gtatggcgag
     3061 aatcatcatg aagagaaacg ttttaatatt tttatatcaa ttccctgtga ttggggtgat
     3121 gacgttacga cgcctcgtcg gcaaatatat atgtctaact caacgacgtt tgatgatcag
     3181 ggttttgcct caaataatac gggattatca ggaacagtag ggagtcggga tcagttcaat
     3241 tatggtgtca acctgagtca tcaacatcag ggaaatgaaa cgacagctgg ggcgaatttg
     3301 acctggaacg cgccggttgc gacagtgaat ggcagttata gtcagtcgag tacttatcga
     3361 cagactggag ccagtgtttc agggggcatt gtcgcctggt cgggtggcgt taatctggcg
     3421 aaccgtcttt ccgaaacgtt tgctgtgatg aatgcgccag gaattaaaga tgcttatgtc
     3481 aatgggcaaa aatatcgcac aacaaaccgt aatggagtgg tggtatacga cggaatgaca
     3541 ccttatcggg aaaatcacct gatgctggat gtgtcgcaaa gcgatagcga agcagaatta
     3601 cgtggcaacc ggaaaattgc cgccccttat cgcggcgcgg ttgtactggt taattttgat
     3661 accgatcagc gcaagccctg gtttataaaa gcgttaagag cggatggtca accattaacg
     3721 tttggttatg aagtcaatga tatccatggt cataatattg gcgttgtcgg ccagggaagc
     3781 cagttattta ttcgcaccaa tgaaataccg ccattggtta atgtagcaat tgataagcaa
     3841 caaggacttt catgcacaat caccttcggt aaagagattg atgaaagtag aaattatatt
     3901 tgccagtaat agtcaggtgg ttctatggaa attcgcataa tgctatttat attaatgatg
     3961 atggttatgc ctgtgagcta tgcggcatgt tatagtgagt tatctgttca gcacaatttg
     4021 gttgttcagg ggaattttgc acttactcaa acacaaatag cgacatatga gcataatttt
     4081 aatgattcgt catgcgtgag tacaaatact atcaccccta tgagcccatc ggatattatt
     4141 gttggacttt ataacgatac cattaaatta aatttacatt ttgaatggac caataaaaac
     4201 aacatcacgt tgtcaaataa tcagagcagt ttcaccagtg gttattcggt aacggtgaca
     4261 cctgcggcca gtaatgcaaa agtgaatatt tctgcggggg gcggtggttc agtgatgatt
     4321 aatggtgttg cgacattatc cagtgcttca tcatcgacac gcgggagtgc cgcagtacaa
     4381 tttctactgt gtttattagg tggcaagtca tgggatgcat gtgtaaatag ctacagaaat
     4441 gcattggcac aaaatgcagg tgtctattcc ttcaatctga cattgtcata caacccgata
     4501 accacaacct gcaaacagga cgatttatta attactttag acagtattcc cgtttcacaa
     4561 ttaccagcca caggtaacaa agcaacaata aatagtaaaa aaagggatat tattctgcgt
     4621 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     4681 agttctgact tgttaaccaa cagcgacaca atactgaaag gtgcggaaga taatgcgtag
     4741 gatttattct tgaaagtaat ggttcgccag tcacactttt aaatatcact aacagcagta
     4801 aaggatacac aaatttaaaa gaaattgcgg caaagtcaaa acttacagat acaacggttt
     4861 caattccgat aacagccagt tactacgtct acgatacaaa caaagttaaa tctggcgcac
     4921 tggaggcaac cgcattaatc aacgtgaaat acgactaata acaagcaaga cgagcaagtg
     4981 gctaataata aaaaataaca aggtgtctgc atttccccct catgatgagg gggctttttt
     5041 tagcgatgat aaaaaatctc accgtcgtag gctttaatga ttttaccgtc ggtgtcgctg
     5101 atcaacacgt acgcgccacc catataggtc cagtgcgtcc cggcatcagg cgcgggcagg
     5161 ttacgtaact gccactgctt aatgttgtac tcatcggtgc ggtacatctc tgggacggta
     5221 tcacctggtt tgaaatgctt aaagtcagca atgaaactac ttaactcata cttagaaatt
     5281 cccgaggtat tggtcgaacc cgcggtggct ggtgcggccc aggcggcact ggtaaccagc
     5341 aaaagcacac ccagcaacat cttattttta atggtcataa actctccact ttggggatct
     5401 gtaatatcag tcgcatatct ttcctgccat acaatataac tgacaagcga tattttgtta
     5461 gtgatcccat gatttcgtaa aaagtgtaaa tgaaaggatg ccctaaaagc atccttagcg
     5521 agcagagagt cagcggaaat agctgattta agaatcatcc tgggctgaag ataacatgca
     5581 agcctaaagg attgagttat cgcattatgc ccatgaagcc atgtacgccc atatacacac
     5641 cgactaagcc aatcaacaga ctggaaaaat agggggcgcg tttagcgaga gtgttaaatc
     5701 cgctccagcg ttttgcgacc tgctgaacgc tgattgctgc gccaacgcct acggtgacaa
     5761 gcgttaacgc caggccaatg ctgaaactga cgaccagtgt tgcgcccagt gtcagggctt
     5821 tcaactgaat gcatatcaac agcacggtaa ttgctgccgg gcaggggata aggccaccgg
     5881 ttaagccaaa taacaaaatt tgccagttga tgacctctct accatcaaag cgtcgtttaa
     5941 tgtcattggc atgggctcgt gcatgggcat cctgatactc gccatgttcg tgatggtgat
     6001 gatgtccttg gtcgtggtga tgttcgtggt cttgatgatg ttcgtgatcg tgatgatggt
     6061 gatgatgctc ataatcatgc ccgtgcatat tctccagcca gttgcgttcg ccgcgccagg
     6121 tacgccagaa catccagaac acggtgccaa taatgatcac tgcggaaatc agctggagcc
     6181 acggttctgc tgattgagca gtaaagcgct tactgatcac catcccgcca aaggcaatca
     6241 accagaccac cgcggtatgc gaaatggttg ctgccagtcc gagcatcacc gcctgtttaa
     6301 tggtgccttt gatggcgatg ataaacgccg ccatcatcgt ttttgagtgc cctggttcca
     6361 ggccatgaag cgcaccaagt aagatggcgc tggggatgaa gaaccaggcg tttccttgct
     6421 gaagaagagt tgtaaattcg gtcatgataa taattcttag tattaattcg gcaatctgat
     6481 tctactcccc cccagtacct gatactaccc cccagtagat taatagtgct atgatttttc
     6541 atattcttgt taaccaggtg ttgccatgtc tcatacaatc cgtgataaac agaaactgaa
     6601 agcgcgtgcc agtaagattc agggccaggt cgtggcgctc aagaaaatgc tcgacgagcc
     6661 gcacgaatgc gctgcagttt tacaacagat tgctgctatc cgtggagcgg taaacggtct
     6721 gatgcgggaa gtgattaaag gtcatctgac ggaacacatc gttcaccagg gggatgagct
     6781 aaaacgtgaa gaagatctgg atgtcgttct gaaggtgctg gattcatata tcaaataatt
     6841 tattaacgcg attgtaaaac tgccgttttt cctcgtttac aacgggtgcg ctggacatta
     6901 ccatcctcct ctgcgattta tcatcgcaac caaacgactc ggggtgccct tctgcgtgaa
     6961 ggctgagaaa tacccgtatc acctgatctg gataatgcca gcgtagggaa gtcacggacc
     7021 accaggtcat tgcttcttca cgttatggca ggagcaaact atgcaagtcg acctgctgag
     7081 ttcagcgcaa tctgcgcacg cgttacacct ttttcaccaa cattcccctc ttgtgcactg
     7141 catgaccaat gatgtggtgc aaacctttac cgccaatacc ttgctggcgc tcggtgcatc
     7201 gccagcgatg gttatcgaaa ccgaagaggc cagtcagttt gcggctatcg ccagtgcctt
     7261 gctgattaac gttggcacac tgacgcagcc acgcgcacag gcgatgcgtg ctgccgttga
     7321 gcaagcaaaa agctctcaaa cacgctggac gcttgatcca gtagcggttg gtgcgctcga
     7381 ttatcgccgc catttttgtc atgaactttt atcttttaaa ccggcagcga tacgtggtaa
     7441 tgcttcggaa atcatggcat tagctggcat tgctaatggc ggacggggag tggataccac
     7501 tgacgccgca gctaacgcga tacccgctgc acaaacactg gcacgggaaa ctggcgcaat
     7561 cgtcgtggtc actggcgagg tggattatgt taccgatgga catcgtatca ttggtattca
     7621 cggtggcgat ccgttaatga ccaaagtggt aggaactggc tgtgcattat cggcggttgt
     7681 cgctgcctgc tgtgcgttac caggcgatac gctggaaaat gtcgcatctg cctgtcactg
     7741 gatgaaacaa gccggagaac gcgcagtcgc cagaagcgag gggccaggca gttttgttcc
     7801 acatttcctt gatgcgctct ggcaattgac gcaggaggtg caggcatgaa acgaattaac
     7861 gctctgacga ttgccggtac tgatccgagt ggtggtgcgg ggattctggc cgatcttaaa
     7921 accttctcgg cacttggcgc ttatggttgc tcagttatta ctgcactggt ggcgcaaaat
     7981 acccgtggcg tacagtcggt gtatcgcatt gagcctgatt ttgtcgccgc ccagctcgat
     8041 tcggtgttca gcgatgtgcg aatcgatacc actaaaatcg gtatgttggc ggaaaccgat
     8101 attgttgaag cggtggcaga acggttgcaa cgttatcaga tccaaaacgt ggtactcgac
     8161 accgttatgc tggcaaaaag cggcgacccg ctgctttcac cttcggcggt tgctacgctg
     8221 cgcagtcgat tattgccaca ggtttcatta ataacgccaa acttgcccga agctgccgcc
     8281 ttgctcgacg cgccacacgc gcgcaccgaa caggaaatgc tggaacaagg gcgatcgctg
     8341 ttggcgatgg gctgtggcgc agtgctaatg aaaggtggtc atctggatga tgagcaaagc
     8401 ccggactggc tgtttacccg cgagggtgaa caacggttta ccgcaccgcg cattatgacc
     8461 aaaaacaccc acggcactgg ttgtacactc tctgcggcgt tggctgcact acgcccgcgc
     8521 catacaaact gggctgacac cgtacaggag gcaaaaagct ggctttcatc ggcgttagcc
     8581 caggccgaca cgctggaagt tggtcacggt attggtccgg ttcaccactt ccacgcctgg
     8641 tggtgagcac cgtactatag ctgctttggt tattgtcagc ctttggcact gacattcagg
     8701 gatgaaacca atgcagctaa gaattaccag tcgaaaaaaa ctcaccgctc tattatgcgc
     8761 tcttgggcta atttccatcg ttgcaattta tccgcgtcag acggtgaatt ttttctattc
     8821 gacagcagtt cagattacag actacatcca cttctacggt tatcgcccgg ttaaatcttt
     8881 cgctattcgc attcctgcca gttacaccat tcacggatta gatgtttcgc gctggcagga
     8941 gcgtatcgac tggcagcggg tggcaaaaat gcgcgacaac gatatccgct tacagtttgc
     9001 ttttattaag gctacggaag gcgaaaagct ggtggacccc tatttttcgc gtaactggca
     9061 attaagccgc gaaaatggcc tgctgcgcgg ggcgtatcat tatttctccc cgtcggtatc
     9121 cgcttcagtt caggcgcgat tatttctgca aacggtggat ttctcacaag gcgatttccc
     9181 tgccgtgctg gacgtagaag aacggggaaa attatcggca aaagaattac gcaagcgggt
     9241 aagtcagtgg ctaaaaatgg tcgaaaaacg cacgggaaaa aagccgatta tttactcagg
     9301 agccgttttt tatcacacca atctggcggg ctatttcaat gaatatccgt ggtgggtggc
     9361 tcactattat caacgtcgtc cggacaatga cggcatggcc tggcgcttct ggcagcattc
     9421 cgaccgtgga caggtagatg gcatcaatgg tccggtggat tttaatgtgt ttaatggcac
     9481 ggtggatgag ttgcaggcat tcgttgatgg gattaaagaa acgccttaaa taagaagcag
     9541 tgatatactc ccaccagact ccctggcagg aaaaagacaa atggaacaag cgcataccca
     9601 gctgattgcc caactgaacg agcgtatttt agcggcggat aacacgccgc tttatattaa
     9661 gtttgccgaa acggtaaaaa atgccgtgcg cagcggggtg ctggagcatg gcaatatttt
     9721 gcccggtgag cgtgatttaa gtcagttaac cggcgtttcg cgcattacgg tgcgcaaggc
     9781 gatgcaggcg ctggaagaag agggcgtggt gacgcgttcg cgcggttacg gcacgcagat
     9841 caacaacatc ttcgaatact cgttgaaaga agcgagaggt ttttctcagc aggtggtatt
     9901 gcgcgggaaa aagcccgata cgctatgggt taataagcgt gtcgtgaaat gtcccgaaga
     9961 agttgcgcag cagctggcgg tcgaagcggg aagtgatgtc tttttgctta agcgtattcg
    10021 ctatgtcgat gaagaagcgg tatcgattga ggaatcgtgg gttccggcgc atttaattca
    10081 tgatgttgat gccatcggaa tttctcttta tgactatttc cgcagccagc acatttaccc
    10141 acaacgtacg cgttcccgcg ttagcgcccg gatgccggat gccgagtttc agtcacatat
    10201 tcagttagat agcaaaatac cggtgctggt gatcaagcaa gtggcgcttg accaacagca
    10261 acggcctatt gagtacagca ttagccactg tcgcagcgat ctatacgttt ttgtgtgcga
    10321 ggagtagttc ctcgcgcgtt ggcgcacaat caccgccccg atgcccgaca acccacgacg
    10381 ccactgcatt gcccagtaat acggcatccg ccagtggcag accagaggcc agcccggcaa
    10441 gtacgccacc tgcatgactg tcgcccgccc caatggtgtc tacaacttgc gttggaaatg
    10501 cccgaatgca gccagaagcg tcgttgctga aataccatgc gccttcttta tcgagacgaa
    10561 cgatcaacgg cgcggcaaat ttctcctgcc attgcttgcc aagtgttgtt atctctgcag
    10621 ataaagcaaa acgttcggcg gcaatctcag cctcttgacg attgagtgac actaaaggtc
    10681 gacaggccat gatccgcgcc agtaatgcgt ccgggatatc gccaatacgt gggccaaaat
    10741 cgataaacgg cgtcacgtct tgcagctttt ccagccattc cactaacaat tcgccgcagg
    10801 gcgaggccag ttgataaccg gaaaaataga gcaggttgcc aggcgcaacg gttaatcgtg
    10861 ccaaccattg gcgattccat tgattttcca caccgctgaa tgacataaaa gtgcgttcgc
    10921 catccggctc aaccagtgcc agacaccagc cgttatcgct ttcggcgtta tcgatcaggc
    10981 tgattaaccc ctctttcgcc atccggttgc gaataatctc cgcccacacg ccctgaccga
    11041 gcggcaaggc attacccgct tcgatgccga ggcgctttaa cgccacggca atattcagtg
    11101 cgcagccgcc aacgttaacg ctctgctgtt tcagttcgat atcgcaccca cgccagggga
    11161 gggcataagc gtcggcgatc acgtcaatga ccgccgcgcc gacgaccatc accggctgac
    11221 gcgtggttaa ttcaggcagc agtgtgtgta atcgagcgcc gctcataccg cctcccgttg
    11281 ttgacgatat ttcgccagcg ctgtggcata gcggttgaaa tcaagctgat ttaccgcatc
    11341 cagctccgcc tttaacgcag gatcgacagc gttaatgcca tgcaacgcgc cgcaaatcgc
    11401 cgtcgccata gcacctatgg tgtctgtgtc gccgccaaga ttggcgcaca ggacggcgca
    11461 gcggtttggg tcggtttgtg ccagttcaac tagcgcaatg gcgcacggaa cggactcaat
    11521 agtgctggtg cctgcgccaa cgacctggta aagctgttcg ctggcggatt cggtgccgtc
    11581 ggcattacgc acaattttca gcgcaatctc cagacgtgcc gccagtgagg cgctgaacgt
    11641 ggtggtgcgt ttttgctgtg catgtcgcgc aattgaaggc aatgaatcaa caatcgctga
    11701 ccagcgttct ccgtcaatgg cacgagaaat cgcccatgcg atgactaccg cgcctgcaac
    11761 cgccagatcg gatttatggg tcgggctgga cgccagcgcc acatcatcaa taaaggaatc
    11821 aacatcacgc gctggaagca aacaacctaa tggcgagacg cgcatcgctg cgccgttggt
    11881 tacgccgttg ttttccagtt cagcaacggg tttaccgtcg cgaatggcgt taagcgcaat
    11941 cttcgaggtc ggacctagta cgtttttgtt aaaggcgtcg aaacgcaacg cccagtcgag
    12001 aatattacgc ccaatcagat tcggatcgat cttgccttca cattccagta acgcatccgc
    12061 cagacacagg ccatcgaggt atcgtcggtg aattcggcgc ggttaaaata acaggccgcg
    12121 ttattctcct ttggtccagg cagaaaacgg tcaatccagc caaagtgtgc tttaacgcgg
    12181 ctgcgtggcc aaagctcgga gggcatcccc atcgcatccc ctaacgcctg cccataaaga
    12241 gcaccgagaa tacgttctgt tttcatttaa cttccccttg tgtcaacgca atatctcgat
    12301 catcgacctt gatagccgta atttcgttgt cggattcgcg gaaaaaaatc atgaacagca
    12361 cggcgataat ggcaatcatc accgcgccga aagtccacat cccggaccag ttgaaagtca
    12421 gtccgtttac cggttcctga taagcgaaca tcttttccat catcacaccg ccaagacgat
    12481 agccgagcaa actgccgaag ccctggcagc agagcgtgat cagcccctgc gcagcggtac
    12541 gcatatgcac gggggctttt ttatcgacat agatgtaagc ggtaacgtag taaaaatcgt
    12601 aacttacgcc gtgaagcaaa ataccgagga acagtaacgc gtaggtgaaa ttgacaggta
    12661 atgtaaggta cgcgatgaca ataccacata ataatacctt ttgaatacct ttgcggtgaa
    12721 aacttgaccg cgctaacatt ttttctgatg aatcgagcca acagaaaacg ctgaaaaaac
    12781 atccaaaaga tggaaaaact cgcccgtaac gaccaggatt atgattgccc gcaaaaggcg
    12841 ggccaggaca atccatagcc gatatccaat cggaatttac gggagcatag taatgacaga
    12901 tattgcgcag ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgactat
    12961 tccttctgac cagctttatc tccccggaca tgactacgta gaccgcgtga tgattgacaa
    13021 taatcgcccg ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc
    13081 tggcacagga tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc
    13141 ttcatttgct gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga
    13201 agcgggctgt aactgtgtgg catcaactta cggcgtgttg gtgtcggtat cgcggcgcta
    13261 tgcgcatcgc attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa
    13321 tacctacgat caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc
    13381 ggttggtgcg actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc
    13441 tgcggccttt gaacgcgcgc acgagctggg catggtggca gtgctgtggg cctatttgcg
    13501 aaactccgcc tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgactggtca
    13561 ggcgaaccat ctggcggcaa ccataggtgc agatatcgtc aaacaaaaaa tggcggaaaa
    13621 taacggcggc tataaagcga ttaattacgg ttataccgac gatcgcgtgt acagcaagtt
    13681 aaccagcgaa aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg
    13741 ggctgggttg ataaactccg gcggtgctgc aggcggtgaa actgacctca gcgatgcagt
    13801 gcgtactgcg gttatcaaca aacgcgcagg cggaatgggg ctgattcttg gacgtaaggc
    13861 gttcaagaaa tcgatggctg acggcgtgaa actgattaat gccgtgcagg acgtttatct
    13921 cgatagcaaa attactattg cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg
    13981 gataaggcgt ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat
    14041 catgcctaca tagcactgcc acgtatgttt acaccgcatc cggcataaaa acacgcgcac
    14101 tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc gaacaatttt
    14161 catattgtcg tttttgtgat cgttatctcg acatttaaaa gcaaataatt tcattatatt
    14221 ttgaaatcga aagcaaacga caagatatga aaatgtacgt ggtatcgaca aagcagatgc
    14281 tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac aatctcgaaa
    14341 cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc atcgccggaa
    14401 cgcctggcac atttactcat gctggtacag aaaatctgtt ggcgctggtc agcgcgatgg
    14461 cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa tttgacgata
    14521 tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg catttgcctt
    14581 ttgcgcaaaa tatttcacgg ctcaaagagg tggtggattt ttgccatcgc tttgatgtca
    14641 gcgtcgaggc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa gtcaatgaag
    14701 ccgatgcgtt gtacaccaac ctcgcacagg cgcgtgaatt tgccgaggca accgggattg
    14761 attccctggc ggtcgccatc ggcacggctc acggtatgta tgccagcgca ccggtgcttg
    14821 atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg ctgcatggcg
    14881 cgtcaggatt atcgactaag gatattcagc aaaccatcaa actggggata tgcaaaatca
    14941 acgttgcaac ggagctgaaa aatgccttct cgcagtcgtt aaaaaattac ctgaccgagc
    15001 accccgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca atgcgcgatg
    15061 tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc acctgccatt
    15121 taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga acatatcggc
    15181 atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc atttgatcgc
    15241 aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca atttggcggt
    15301 tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc cgataaagtt
    15361 gggtttgcac gcgaacgcat tattctcggc ggcgatcatc tggggccaaa ctgctggcag
    15421 caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc atatgttcgt
    15481 gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga tcccataccg
    15541 ttagcaccag aaacggttgc ggaacgagct gttgtgcttt gctttgctgc ggaaagtgtg
    15601 gcgacagatt gccagcgtga gcaactgagc tatgtcattg gcactgaagt tccggttccg
    15661 ggcggtgagg ccagcgccat tcagtcagta cacatcaccc atgttgaaga tgccgccaat
    15721 actttacgta cgcatcaaaa ggcctttatt gcccgtgggc tgacagaggc gttaacacgt
    15781 gtgattgcca tcgtggtgca gccgggtgtg gaatttgatc acagcaatat tatccattat
    15841 cagccgcagg aagcgcaggc gctggcgcaa tggatagaaa acacccgaat ggtttatgaa
    15901 gcacattcta ccgattacca gacccggacg gcttattggg aattagtccg cgatcacttt
    15961 gcaatattga aagtcggtcc cgcattaacc tttgctttac gcgaggcgat atttgcactg
    16021 gcacaaattg agcaggaact tatcgccccc gaaaatcgca gcggttgcct ggcggtaatt
    16081 gaagaagtga tgctcgacga accgcaatac tggaaaaaat attatcgcac gggttttaac
    16141 gattcattac tggatattcg ttacagcctg tcggatcgta ttcgttatta ctggccgcat
    16201 agtcggatta aaaatagcgt tgaaacgatg atggtgaatc tgcaaggcgt ggacatccca
    16261 ctgggcatga ttagtcagta tcttcccaaa caatttgaac gcattcagtc cggggaatta
    16321 tcagcaatgc cgcatcagct tattatgaat aaaatttatg atgttttgcg cgcctatcgc
    16381 tacggctgtg cggaataagg acggtatatg actaacctgt ttgttcgtag cggaatttct
    16441 tttgtcgatc gtagcgaagt tttaacctat atcggtaatg agatgctcgc gaaaggtgtg
    16501 gttcatgata cctggccaca ggcattaatt gccagagaag cagaattccc taccgggata
    16561 atgcttgagc agcacgccat tgcaataccg cattgtgagg cgattcatgc taagtcgtca
    16621 gccatttatc tgttaaggcc aacaaataaa gttcattttc agcaagcgga tgatgataac
    16681 gacgtggcgg tatcgttggt tattgcgttg attgtggaaa atccgcagca gcaattgaaa
    16741 cttttacgct gtttatttgg caagttacaa cagcccgata tcgtcgagac actaattact
    16801 cttcctgaaa ctcagttaaa ggaatacttc acaaagtatg ttttagattc agacgaataa
    16861 atccctctgt aacaataaaa aggactattt atgaaacgca agattattgt cgcttgcgga
    16921 ggcgcggttg cgacctctac gatggcggcg gaagaaatta aagagttgtg tcagagtcat
    16981 aatattcctg ttgaattaat ccagtatcgg gttaatgaaa tagaaaccta tatggatggt
    17041 gtgcatttga tatgtaccac tgccagggtg gatcgtagtt ttggcgatat tccgttagtt
    17101 cacggcatgc cttttgtttc tggtgtcggt atcgaagcat tacaaaataa aattctgact
    17161 atcttacagg ggtgacctat gttttcagaa gtcatgcgtt atattctcga cctcggccct
    17221 acagtgatgc tgccggttgt catcattatt ttttctaaaa tattaggcat gaaggcaggc
    17281 gattgcttta aagcgggtct gcatatcggg attggctttg ttggcattgg ccttgtgatt
    17341 ggcttaatgc tggattccat tggtccggcg gcgaaagcga tggcggaaaa tttcgacctg
    17401 aatctgcatg tggtcgatgt cggctggccg ggctcttcac caatgacctg ggcgtcgcaa
    17461 attgcgctgg tggcgattcc gattgcgata ttggttaacg tggcgatgtt actgacccgt
    17521 atgacgcggg tggtaaatgt tgatatctgg aatatctggc atatgacctt caccggcagc
    17581 gttgctgcac ctggcaaccg gttcatggat gatagggatg gcgggtgtgg taattcacgc
    17641 ggcgtttgtt tataagctcg gcgactggtt tgcccgcgat acccgaaatt tctttgagct
    17701 ggaaggtatt gctattccgc acggtacgtc ggcgtatatg gggccgattg cggtgctggt
    17761 cgatgctatc atcgagaaaa tcccaggcgt taaccgaatt aaatttagcg ccgacgatat
    17821 tcagcgcaaa tttggccctt ttggcgaacc tgtcaccgtg ggttttgtga tggggctgat
    17881 tatcggcatc ctcgcgggtt acgatgtcaa aggtgtattg cagctggcgg taaaaacggc
    17941 ggcagttatg ctgctgatgc cacggatgat taaacccatc atggatggtt taacgcccat
    18001 cgctaagcag gcccgtagtc gtttacaggc gaagttcggc ggtcaggagt tcctgattgg
    18061 cctggatccc gcattactgc tggggcatac ggcggtggta tcggcaagcc tgatttttat
    18121 tccgctcacc attttaattg ctgtttgtgt tccgggtaat caggtactgc cgtttggcga
    18181 tcttgccact atcggcttct tcgtggcgat ggcggtcgcg gtgcatcgtg gaaatctgtt
    18241 ccgcacctta atctcgggtg tcatcattat gagcatcacc ctgtggatcg cgacgcaaac
    18301 tattggtttg cacacccaac tggcggctaa tgctggggcg ttaaaagccg ggggtatggt
    18361 ggcttcaatg gatcagggcg gttctcccat tacctggtta ctgattcagg ttttctcccc
    18421 gcaaaatatt cccggtttca ttattatcgg cgcaatttat ctgaccggta ttttcatgac
    18481 ctggcgtaga gcgcgtggtt ttactaaaca agagaaagcc gttctcgcag aataattttt
    18541 acctgagggg gaggttatcc cccttaacaa tcaggagttt ttatgaaatc agtagtgaat
    18601 gatactgatg gtatcgtgcg cgttgcaaaa agcgtcattc ctgaaattaa acatcaggat
    18661 gaggtgcggg taaaaattgc cagctcgggc ttatgtggtt ccgatttacc caggatattt
    18721 aaaaatggtg cacattatta tccaataacg ttaggccatg aatttagcgg ctatattgat
    18781 gcggtgggat ccggtgttga tgatttacac cctggcgatg cggttgcctg tgtgccgtta
    18841 ttaccctgtt ttacttgtcc agagtgtttg aaagggtttt attcccagtg cgcaaaatat
    18901 gattttattg gctcgcggcg tgatggtgga tttgctgaat atattgtcgt taagcgaaaa
    18961 aatgtctttg ctctacccac ggatatgcct attgaggatg gggcttttat tgagccgatt
    19021 accgttggcc tgcatgcttt tcatttagcg caaggttgtg agaataaaaa cgttattatt
    19081 attggtgccg gaaccattgg cctgctggct attcagtgcg ctgtcgcgct gggagcaaag
    19141 agtgtgacgg cgatcgacat tagctcagaa aaactggcac tggcaaaatc tttcggtgcg
    19201 atgcaaacat ttaacagtag cgaaatgagc gcgccgcaaa tgcagagcgt tttacgcgaa
    19261 ctgcgcttta atcagcttat cctcgagacg gctggcgtac cgcaaactgt cgaactggcg
    19321 gtagagattg ccggtcctca tgcccaactg gcgctggtgg gcacgttgca tcaggatctg
    19381 catttaacat cggcaacgtt tggcaaaata ttgcgtaaag agctgacggt tatcggcagt
    19441 tggatgaact attccagccc ttggccgggg caggagtggg aaacggcgag ccggttgctg
    19501 acagaacgta agttaagcct ggatccatta atcgctcacc gtggaagctt tgaaagcttc
    19561 acccaggcgg tgcgtgacat cgctcgtaat gccatgccgg gcaaagtgtt gctcattcca
    19621 tgaaaccgcg ggccagcgtg atgctggccc ggtattgtgc aaaacagatc attcaccaat
    19681 ggtccccctt cgtttacact agccacaatt gaatatgggt aaatgacgat gaattcattc
    19741 gagcgaagga ataagatcat ccaattagtg aatgaacagg gaaccgtgct tgttcaggat
    19801 ctggcgggag tatttgctgc ctcggaagcg acaatccgtg ccgatttgcg ctttctcgaa
    19861 caaaaaggcg tggttacgcg ctttcatggc ggtgcggcga aaataatgtc cggtaatagt
    19921 gaaaccgaga cccaggaagt cgggtttaaa gagcgatttc agctcgccag cgcgccaaaa
    19981 aacagaatag cgcaggaggc agtcaaaatg atccatgaag ggatgaccgt tattctcgac
    20041 agcggaagta ccacaatgct tatcgccgaa ggattaatga ccgctaaaaa tatcacggtg
    20101 attaccaaca gtctcccggc agcgtttgcc ctttccgaaa ataaagacat tactctggtc
    20161 gtctgtggtg gcacggtccg ccataaaacg cgctcgatgc atggttccat tgccgagcgt
    20221 tcattgcagg atattaatgc cgatttaatg tttgtcggtg ctgatggtat tgatgcggtt
    20281 aatggtatta cgacctttaa tgaaggttat tcgattagcg gggcgatggt caccgccgca
    20341 aataaagtga ttgccgttct cgattcatcg aaatttaatc gtcgcggttt taatcaggta
    20401 ctgccaatcg aaaaaattga cattattgtt accgatgatg ctgtgtcaga ggtggataaa
    20461 ttggcattgc agaagacacg ggtaaaatta attacggtgt agtattgttc tgtatgccgg
    20521 ataaggtgtt tacaccgcat ccggcattac ggattctccc tggttcaaga gagaatagtt
    20581 tgattaacgc aataatggac aatctggtgg taatcgacaa cgcaacgccg ccggaagtat
    20641 ttcaatatgg aagttttgcc cactcaacgg ttcgccatca agattaaaag tgatttcgtg
    20701 tggggcttgt atatcaaacc acgacgaagc gccttcgata atattcgggt tatcttcgtc
    20761 agattttaag gttgatacga gagccggaag aatttcatcg ccggtaaaaa tgcgcagttg
    20821 cagcaagcca tcgttaatta acgcgttcgg gcacaattgt tgaccgccac cggcctgacg
    20881 cccgttacca ataccaatga ccagggcgtc accttgccag tgaaagtttt caccgcggat
    20941 ttcacaacgg tccggttgca gagtatccat gcgcattaag ccatgaatga tgtaagagac
    21001 gccacccagc gcggctttta atttttccgg cgtttctgtg gtaatacgcg tcccaaatcc
    21061 gcctgtcgcc atattaataa aacaggtttg tttgttgacc tgcgccatat ctatcgcaat
    21121 ggcattaccg gcaattgcca gtttcagcgc cttatccagt gcctcaggaa tccctacact
    21181 ggtggcaaaa tcattggcgg ttcctaatgg caaaattccc agcgcgggta tgtcatcccc
    21241 ctcacactga atcaacgccg tagaaacttc attaatggtg ccatcgccac caccggcaat
    21301 caccgttgcg acgcccaact tccgggcctc ctctacaaat cgtgcggcat cacctttctc
    21361 ccaggtgacc cgcacatgga tcgtcattcc ttcctcacgc aacagcataa ttgcttcgcg
    21421 caagggtaga ttgtcagtac ttttgccatt aagaatcagt aagctggcgg gaaattctgc
    21481 catgatcgta tgtgccttta tgattggtct gtagatagtg tagagcagaa aacaaaaggt
    21541 ggagtcagaa caggatgaaa gtcggaggat aaaaagtcag ttcaagtatg gaggattcag
    21601 tggttaccac caggagcttg caatggggac agcgaatact aacggctgcc accgataaat
    21661 ttcaaaaaag agcatatacc taatattcaa ctaaacagtg gcatcttcaa tataatatat
    21721 taaagccccc atggagttac cctgaagggc ctcaatgtcc gtaattccta cttatgtagg
    21781 aaatgttgta cagaacattt attataatcc tattcaatta taataatcat gccattatta
    21841 tatttaaaca ctagagagtg tcgttggtat ttaatggggg aaggtgagat gaaaaagata
    21901 gctgctatat cattaattag tatttttctt atatctggtt gtgctgtgca taatgatgaa
    21961 acaagtatcg gtaaatttgg tcttgcatat aaaagtaata ttcagcgtaa actcgataac
    22021 caatactaca ccgaagccga agcttcttta gccaggggca gaatatctgg tgcagaaaat
    22081 atagtaaaaa atgatgcagc ccatttctgt gttactcagg gcaaaaaaat gcagatagtt
    22141 gacctgaaga cagaaggtgc aggattacat ggcgtcgctc gtctgacatt caaatgtgga
    22201 gagtgagaat attttttggt aagcgtcaaa catgcgcgtt ctggctgtgc gtagccggaa
    22261 cctgggcgag cacgataccg ttacgtgaaa ggcattgtgc tatgaagggg gattctatcg
    22321 atgtggtcaa tggaagacgg ttatcaggga tcaatccttc tttacagctt atgtacaaaa
    22381 aataagcccg tgtaagggag attacacagg ctaaggaggt ggttcctggt acagctagca
    22441 ttttatggat tatgtttttc agcgaaacgg atgataacct taataaatgc agctgtatgt
    22501 gatcggtttc taagaatttt ccatccggga aaaataatcg aaattaatca cttaccgtgg
    22561 gggttacgcg tggtttcccc ggagaaatta cgcattagca gcgcgtaatt tagctcaaga
    22621 tcctgcggga ccgggagcca cacagtataa ccatcgcctg gtgcgaccgg catagcttcg
    22681 cctttggcgt tttccatgtg ctcaagggta aagttaatgt tgccttgcgg cgtcatcagc
    22741 tcaagactgt cgccaacgga gaatttattt ttcaccgcta ccgctgcgag gtcccccttg
    22801 cgctcaccgg taaactcacc aacaaactgc tggcggtcag aaactgaata accgtattcg
    22861 tagttctgat aatcgtcgtg agtatgacga cgcaggaaac cttcggtata gccacgatgc
    22921 gccagacctt ccagagtttc cagcaggctg gtatcgaacg gttttcccgc agcggcgtca
    22981 tcgatagctt tgcggtaaac ctgtgcggtg cgtgcgcaat agtagaaaga tttggtacga
    23041 ccttcgattt tcagcgaatg cacgcccatt ttggtcaggc gttctacatg ggcgatggcg
    23101 cgcagatctt tcgagttcat gatgtaagtg ccgtgctcat cttcaaacgc ggtcatatac
    23161 tcgcccggac gctgggcttc ttcgatcata aacactttgt cggttggcgc gccgataccc
    23221 agcgtcggct caacattttg caccggaatc ggctcgtact tgtgtacgat gttgccaacg
    23281 tcatcttctt tcccttcctg gacgttgtac tcccagcggc aggcgttggt gcaggtgccc
    23341 tggttcgggt cgcgcttgtt gatatagcca gagagcaggc agcgaccgga gtaggccatg
    23401 cacagtgcgc cgtgaacgaa gatctcgatc tccatatccg gcacctgatt atggatctct
    23461 tcaatctctt ccagcgacag ctcgcgagag aggatcacgc gggtcagtcc catttgctgc
    23521 cagaatttca ccgtcgccca gttcacggcg ttagcctgca ccgagaggtg gatcggcatt
    23581 tctgggaagt gctcacgcac cagcataatc agccctggat cggacataat cagcgcatcc
    23641 ggccccattt ccaccaccgg tttcaggtca cggataaagg ttttcagctt ggcgttgtgt
    23701 ggtgcaatgt tgaccacgac ataaaacttt ttccccagcg cgtgggcttc attgatgccg
    23761 agctgaagat tttcgtggtt gaattcgttg ttgcgcacac gtagggagta acgcggctgg
    23821 cccgcataaa cagcatctgc gccataagcg aaagcgtaac gcatattttt cagcgttccc
    23881 gccggggaaa ggagttccgg tttaaacata attctctcgt tctgatgaca ggtcagatcc
    23941 gcttcacctg atgaagcggt taggggaggc ccccacttta agggcgggca ttgtagcgct
    24001 gtagggggga tctggtaaag cgccggacgc caacaaccca cgcccggcat cgtaattacg
    24061 tattgtcttt caccgtctgg cttgtgccat tggctttaac ggaggcaatt ccggtttcac
    24121 gagattgcgc ggtggcgtac atctggctgg agccgataat ttgatgatta gcggctttca
    24181 gattgaaata gaatttgccg tttttttctc atagcgctct tcttgcgggc tgttgctacg
    24241 caccgacgcg atgccctttt ccgcagaggc ttttgaggta tacagctcgc tggtgaggat
    24301 agtctcacca ttgcccgctt ttagcacaaa ccggaactga ttatcactgc tcttactgag
    24361 ttcaaaccaa ccagccataa tgactccttg attgaaattg cttatcgaaa acagcatcta
    24421 aaggtgctgt ttactgatta aagtatgagc gattttctga tgcctgccag ggcggagaga
    24481 taatgtcagt ttgctgacca tgcgtggaat aatgttgccg gatcgggtct gaacgccatc
    24541 tccggctaac aaagtaatgt cgctaaaact aaacgatgcg gcaggcgtcg gcttcccagc
    24601 ggtaaccaac gccgtaaacg gcgcggataa atgactgttc ggcgtcgaga gattccagct
    24661 tgcggcgcag atttttaatg tggctgtcaa tggtgcggtc ggttactacg cggtagtcgt
    24721 cataaagatg attgagcaat tgctcgcggg agaacacttt tcctgattcg tgagagagcg
    24781 ttttaagcag acgaaattcc gcaggcgtca ggtcaagcat tttaccgcgc catgaagcct
    24841 gaaaacgacc ttcgtcgata atcaacgggc tttcggcatc ctgttgctga agttcgcgtt
    24901 gcggtttaca acggcgcaaa atggttttga cacgcgctac cacttcccgt gggctgtacg
    24961 gcttgcagat gtagtcatcg gcgccaatct ccagccccag caggcggtcg atctcttcga
    25021 tttttgccgt caccatcacg atcggaacat cggaaaaacg acgaatttcc cggcataatg
    25081 tcaggccatc ggtgccaggg agcatcagat ccaacaggat cagatccggt ggtgtctggc
    25141 gcacatacgc cagtacctga tcgccgtggc tgataagcgt cggcgcatag ctcgcagcac
    25201 gcagataatc aatgagcaac tgccccagct tcggttcatc ttccacgatc aaaatacgcg
    25261 gtgtgttttc gtcgattggt aactcggtca tacttctctc tgtaaatccc gttccagcgg
    25321 taactctact gtaatgctta ccccgccaaa aggcgaatgg gcggcaataa tgcggccatt
    25381 atgggcttca acaatgttca ggcaaatcgc cagccccagc ccggaaccgc cgctggcgcg
    25441 gttgcgggaa ccttcggtgc gataaaaacg ttcaaacaat ttttgtagct gatcgtcact
    25501 gacacctggc gcactgtcgg caaaggtcag gcgcaccgtt ttgtcgtgct gctcggcaga
    25561 gattttcagg ctgccgccgc tgtcagtgta gcgcaggctg ttttccagta agttattgaa
    25621 taactgcatt aaacggtcgc gatcgccaaa tacggtgata ctgtctggca gggaaaattg
    25681 cagtttcagg ccacggctgg cgaatcgttc gcgaaatgcg ccgcccgcca cttccagcag
    25741 tgggatcaaa tctaccggtg ctttttgata ggcgagagcg ccttcatcag acatcgacaa
    25801 ctgatgaaga tcgtcaacca gtttggtcag tgtaccgacc tccgcctgta aagacgccac
    25861 cgtctccggc gtgaatttgc gcacgccatc ctgaatggct tccagttcac cgcgcagcac
    25921 cgctaatggc gtacgcagtt cgtgagaaat atcggccata aaatcgcgcc gcatttgctg
    25981 gtttttctcc agcgtgctgg cgagctggtt gaagtcttgc gccagtttgc ccagttcatc
    26041 ttcactggtg ggcgttacgc gggtagtgaa atcgcccgcc gccagtttgt gcgtgccatc
    26101 gataagtcgt tttaccggtg ccagcaaacc gcgcgccagc agaaaggtgg cgagcgccgc
    26161 gagtaacgtt gccagggcga cgatcagcca gctggtttgc cgctgttgtt tatcgaaatt
    26221 gatatcagta ttgcgggtca gccgctcaac aggggaggca atcaccgcgc ccacttccgc
    26281 accgttgacc agaatgggtc gtcgtgtacc gtcaggtgga atcggcgctc gcggaccaac
    26341 cagtactttg ttgttttgat cgaccaccca gaactgggta cgccagccgt gcggcggcat
    26401 acccgggccg ggtttatctt ccgaattatc gtgttcaaat gaacgcagga tctgaaagac
    26461 aaagcgatcg ttgttgcgca ggaagcgcca gttaccatgc tgcgcatact gctcgccaag
    26521 cgcatcactt aacagctgta atcgctgttc attgccgtgt ttgatgtaat caataaagcc
    26581 acgctcaaaa ctgatacgca ccgcccagtg catactgatc agcaagacaa tgcaggtggc
    26641 gaaaatcgcc agaaacagtt tgccggtaat accgggtcgc cagaacttca ttgcgcgctc
    26701 ctttttcgcc gcgaaatagc tacattttga tgcgtatcgt tcggcactct ggcaaagatg
    26761 aacgccggaa gggcgatgat aaacgccatg ctaagccagg tgtacataaa gacggtttgt
    26821 gtggtgccgc tgtcgacgct gacatgctgc gaaccaaaaa gccccagcaa cagcccggcg
    26881 atggtgacgc cgatactcat cgacaattgc ataatcatcg acagcaggct gtttccgctg
    26941 ctcgccagat tgtccgggag atctttcagc gtcagggtgt tcatggagga gaaacgggtc
    27001 gagttgacca tcccttgtaa aaacaggacg aacggcaaaa cgtagtacca gcccaacaga
    27061 gcggtagtca taaacaacag ggtgaccagc gacagaccca gcgtggtcgc taccagtacc
    27121 cgacgataac caaagcgatt caccacctgt accacaattc gcttcattcc catgctgcca
    27181 agcaccatcg ggatcatcat cagtccggca tgaaacggcg agaaaccgag gccaatttgc
    27241 aggaaaaccg gtgtcataaa gggcaacatg ccactgccaa tacgtccggc aaagctcccc
    27301 gccaggccca gcgaaaaggt acgagtgcgg aacagtttca gactgaacag ggcgcggttg
    27361 ttatttctgg cgtgcagcag ataaagcacc agtgccacca cgccaactgc gaccaggcca
    27421 gcaatcgcca gcggcgataa acctgtacct ttactgccgt ccagcgccag ggttaatacc
    27481 gccatgccaa ccgccagcaa taaaaagccg gaaagatcaa agcgccgcgt ctgcatggtg
    27541 tagtttggca ttaacatcaa tgtggcgatc gcaccgataa tccccaccgg aatgttgatc
    27601 aaaaagatcc agtgccacga cgcgtattct accagcagac cgccgagcgc cggaccgagt
    27661 agcggaccga cctgaccggg taacgtgaca aaggtcatcg ccgccatata ttgctcgcgc
    27721 ggtacgattt tcatcaccgt caacctgccg accggcacca tcatcgcacc gccaacgccc
    27781 tgtaacgcgc gtgccagcaa cagttcgttc agcgtgccgg aaagcgcgca aaacagtgaa
    27841 ccgagagtaa acagcacgat ggcggtaaag aaaatattgc gcacgccgac tttgtccgcc
    27901 agccagccgc tggcgggcag catcaccgcc acggtcagca cataagagac aatgaccatg
    27961 tgcatatgca acggactttc cccgaggctt tgcgccattg agggaagggc ggtgtttacg
    28021 atggtggtgt ccagcgactg cataaagaag ccgaaagcca caatccataa ttgccagcgg
    28081 gtgctgtcgg gaagatctgt catttactcg gttaccgttt gtttaggttt acgcgaaaaa
    28141 cgcagccgca gacggtcgaa aaagagatac accaccggcg tggtatacag cgtcaggagc
    28201 tggctcatca ccagaccgcc gacaatggtg atcctcaggg gttgccgcag ctccgagccg
    28261 tcaccgcccg acaataccag cggcagcgcg ccaaacagcg ccgccagggt ggtcatcata
    28321 atcgggcgaa aacgcagcag acaggcctgg aaaatagctt cctgcggcgt caggttaccg
    28381 tgccgttggg cttcaagcgc aaaatcgacc atcataatgg cgtttttctt cacgatgccg
    28441 attaatagca tgatccctat cagggcgatt aggctgaacg gggcattgaa cagctccagc
    28501 gccaacagcg ctccaacgcc cgccgagggc agggtggaga gaatcgtcag cggatgtacg
    28561 taactctcgt aaaggatacc cagcacgata tacaccgtgg cgatggcggc gataatcagg
    28621 atcacctgcg agttcatcgt ctcctggaaa acctgcgccg tgccggcaaa actgccgcgc
    28681 accgtcgaag gcacaccaag ctgggtcatt gcgcgatcga tcgccgcact ggcgtccgag
    28741 agcgattttc cggtcggcag gttgaacgag atggtcgagg ccgccgataa tccctgatga
    28801 ttcaccgata gtggggcatt cgccggttgc catttagcga aatacgacag cgggatcgct
    28861 ttgccttcgt tattgataac gaacattttt tccagcgcac tgatgtcctg ggtatagcgc
    28921 ggatccactt ccatcaccac tttatactgg ttcatcggct ggtaaatggt cgagatttgc
    28981 cgctgaccga aggcgttatt taacagactg ttggcggcct gtacgtcgat tcccagccgt
    29041 gccatggtgt cgcggtcgta aaccagattc atctccgcgc cgttatcctg ctgatcggag
    29101 ttcacgtccg ccagttccgg caacgtcgcc agttttttgc ggattttcgg ctcccattcg
    29161 cgcagtgccg ccaggtcgtc ggataacaac gtgtactggt agctggcgtt cgactgacgc
    29221 ccaccaacgc gaatatcttg taccgccatc aggaacagat tcgcccccgg ttctttcgcc
    29281 agcttcacgc gcagacggtc gataatttgc tgcgccgttt cgctgcgttc gtcgcgtggc
    29341 ttgagggtga taaacatcat cccactgtta actcgtgaac cgccggtaaa gccggtgaca
    29401 ttatccactg ccggatcgtc acggataatt ttcatgaaat cctgcaattt gccgcgcatc
    29461 gcctgaaacg aaatactctg atccgcctgt atcccaccca tcagtacgcc ggtgtcctgc
    29521 tccgggaaga aggttttcgg gatcgagata ttcagcgcaa tggtgccaag cagcaccacg
    29581 cccaccagac gggtatgatt gagcacccat ttcagcgact tgccgtagcc ttgttgcagg
    29641 gctaccaaca tgcggccaaa accacgcagt cgcttttgct cacgcggctt gctggctttt
    29701 agcatccagc cgcacatcat tggcgttagc gtcagggaaa ccagcaacga aataccaatg
    29761 gcgacagaaa gcgtcacggc aaactcgcgt aacagtcggc ccggcaatcc gcccatcaac
    29821 agcagcggca ggaacaccgc caccagtgac agactcatcg acagcaccgt aaagccgact
    29881 tcgcgagtac cttgcagcgc ggcctgtagt ggtttcatcc ccgcttccag atgacgggca
    29941 atgttttcca gcaccacaat ggcgtcatcc accacgaaac cagtagcaat ggtgagcgcc
    30001 atcagcgaaa gattattgag actgaatccg cacaggtaca tcgccgcaaa cgtaccaatc
    30061 agcgaaaccg gcaccgcaac ggcgggaata atagtggcgc gacccgagcg caggaataaa
    30121 aagaccacca gaatcaccag cgccaccgag ataatcagcg tttgctcgac ttcttccagc
    30181 gaggcgcgaa tggtggggga gcgatcctgg gcaatttgca gatcaatcgc cgccggaatg
    30241 gtctcctgca actctggtaa ttttgcccgg atgctgtcta ccgtctggat aatattggct
    30301 tccggcagtt tgcggatcat cagtaaaata gccggtttgg cgttggtcat cccggcgttg
    30361 cgcacatcct gcactgagtc agtcaccgtc gccacatcgc ccagacgaac cgcgccgccg
    30421 ttgttgtagt gaataatcag cggctgatat tcagcggcgg ttttcagttc atcgttggtc
    30481 tggatctgcc agcggtgagt gccatcttcc agcgccccct gcggtttacg cacgttggca
    30541 ttgctgatgg cagtgcgtac gtcgtccagc gacacgccct gattaaacag cgcctgcggg
    30601 ttcagcccga cgcgcacggc gggcagcgag ctgccaccga catcgacatc accgacaccg
    30661 tcgatttgcg aaatcgtcgg agccagctgc gtcgaggcga aatcgtacag ttcaccctgc
    30721 gaataagtat cggatgtcag cgtgaggatc ataattggcg catccgacgg gttcgctttg
    30781 cgataggtcg ggcggctggg catcccgctg ggcagcaaac tttgtgcagc gttgatcgcc
    30841 gcctgcacat cacgcgctgc gccgttgata tcccggtcaa aatcaaactg caaaataatg
    30901 cgcgtgctgc cgagcgaact gctggaggtc atttcactaa ccccggcaat gcgcccaagt
    30961 tagcgctcca gcggcgtggc aacggaagac gccattgtct ccggcgacgc accgggcagc
    31021 gaggcgctga ccataatcac cggaaaatcg acctgcggca gcggggcgac cggcagcata
    31081 cggaaaccca gtatgccgca cagggtaatg gcgaccgaca gtaaaatcgt cgccaccggg
    31141 cggtaaatga agagggcaaa aaacttcact tacgcctcct cttcatggcg agcaaagcga
    31201 cttttggtcc acaataccaa gcggtcgaac agcagataaa tcaccggcgt ggtgaacagc
    31261 gtcagcacct ggctgacaat cagaccgccg accatgccga tacctaacgg acgacgcagt
    31321 tccgcgccga ccccggtact caacatcagc ggcagcgcgc caagcagagc cgccagggtg
    31381 gtcatcagga tcggacgaaa acgcaacaga caagcctggt agattgcatc gcgcggcgac
    31441 atgccttgct cgcgctcagc agccagcgcg aagtcgatca tcatgatggc gttcttcttc
    31501 acaataccga tcagcaaaat aatgccgata atcgcaatca catccagttc gctaccggca
    31561 atcattaatg ccagcagcgc gccgacccct gcggtgggta gcgtcgagag aatggtgatc
    31621 gggtgaataa agctctcgta cagaatgccg agcacgatat acatcgccac caccgccgcg
    31681 acaatcagcc agacagtgct gcccagcgcc gactggaagg cgagggtgct gccctggaat
    31741 tgcgtggtga tatccaccgg cagattcagc gtcttttcgg tgtccataat cgcctgcacc
    31801 gcatcgccca gcgaatagtt atccggcacg ttaaaggaga tggttgttac cgggaactga
    31861 tccagatggt tgatggaaag cggcgcaaaa cgctgctcaa ttttggcaat tgagcttaac
    31921 ggcaccacgc caccgtcgct gctggtcagg cgaatggtat ccagcgccgc gaggcctggg
    31981 gtattttcgg tattgtgctc cagcaccacg cgatactggt tagcctgagt ataaatagtg
    32041 gaaatcagtc gctgaccaaa cgcgttgtac agggcgttat cgacgtccgc catgctgatc
    32101 cccagacggc tggcgctgtc gcgatcaaca ttgacatacg ccaccagccc tttgtcctgc
    32161 cagtcgctgg agacatcaga aagctgtggc agttgctgga gtttttccat caactgtggc
    32221 acccaggtac tgagcgcatc cagtgacgtg gcctgcaagg taaactggta ctgggtgcgg
    32281 ctgacctgag tatcgatggt caggtcctgc gttggttgca ggaagagatc gacgcccggc
    32341 actttatcta ccgccgtttg cagacgggcg atgacttttt gcacccgatc atcacgttca
    32401 tccaacggtt tgaggttgat ttgtaaacgt gcactattca gcgacgggtt agtgccatca
    32461 acgccaacaa atgaggtcag gctttgcact gccggatcct gcaaaatcac gtccgcgacc
    32521 tggcgttgtc gctgggccat attggcaaag gagctggatt gcggtgcctg caaagtgccc
    32581 tgaataatgc cgttgtcctg taccgggaag aaacctttcg gaatgaacac ccacagcagc
    32641 acgctaagca gcagcgtgct gagtgccacg cttaaggtca gccacggatg attcagcact
    32701 ttcgccagtc cacgaccata ggcggcgatt atcctgtcga acattttttc cgaggcacgg
    32761 gagaagcggt tctgtttacg caacgactcc tggctgagca tccgcgcgca catcatcggt
    32821 gtcagggtca gcgacaccac cgctgagatc aaaatcgcta ccgccagggt aatagcaaat
    32881 tcgcggaaca gtcgcccgac gatatcgccc ataaacagca gtgggatcaa caccgcaatc
    32941 agtgagaagg tcagcgagat aatggtaaag ccgatttcac ctgcgccctt gagcgccgcc
    33001 gccaacggtt tttcgccttt ttcgatatag cgggaaatgt tttcgatcac cacgatggcg
    33061 tcatcgacca cgaatccggt ggcgatagtt aacgccatca gtgtcaggtt attgattgaa
    33121 aaatcgagaa acaccataac cgcgaaagtg ccgattaacg acagcggtac agcaacaccg
    33181 ggaatgatgg tcgccggaat attgcgcaaa aacaggtaga taatcatgac taccagcgcg
    33241 atagccatca tcaattcaaa ctgagtatca tcgacggatg cgcggatatt ggtggtgcga
    33301 tcggaaagca ctgtcacctt caccgatttc ggcagactct cagtgagctg tggcagcatc
    33361 tgccgaatgc tgtcggcggt ggagataatg ttagcaccgg gctggcgctg aacattcatc
    33421 acaatggcct gttctttgtt cgcccacgcg ccgagccagc tgttttctgc accttgctct
    33481 acagttgcta catcgcccag acgaattggc gcgccgttct ggtaggcgat gattagctgg
    33541 cgatactctt cggcggattg catctggtcg ttcgcggaaa gcgtgaccgc acgggaaggg
    33601 ccgtcgaggc taccttttgc cgagttaacg ttagcgccgg taatggcggt gcgcacggtt
    33661 tcgctggtca ggccgagggc ggcaatcgcc tgagcgttaa gtttgacgcg aacagccgga
    33721 cgctgaccgc cggaaagcgt caccaggccg acgccggaaa tctgcgagat tttctgcgcg
    33781 acgcgggttt ccaccatatc ttccacttgc gtcatcggca tggcggttga ggtgacggcg
    33841 agcgtcatga tcggcggatc tgccgggttc actttgctgt aaaccggcgg gttaggcaga
    33901 tcgctcggca acaagttggt cgcagcgtta atcgcggcct gcacttcctg ctcggcgaca
    33961 ttgagcggta atgttagctg gaactgcaaa gtgataactg acgcaccgcc ggaactttgc
    34021 gacgacatct gtttcaggcc agacatctgc ccgaactggc gttcgagcgg cgcggtaacg
    34081 gcagaggtca tgacatccgg gctggcactt gggtagagcg tgaccacctg aatggtcgga
    34141 tagtccactt ccggcagcgc cgaaacgggc agggcgcgat aaccgataat cccggcgagt
    34201 aagatcgcca ccatcagcag cgtggtggcg acaggacgca taataaacag gcgcgacggg
    34261 ccgcctgtgc tgctcggggg taacacctgc atcaggagcg tgctcctttt ttcgcgtatt
    34321 cgcggctggt ggctttctct tccggagtgg tggcgctctg ggcttccacc acttccactt
    34381 tctgactgtc ctgaatgccc ggcgtcacca gatgtttgct gaccttgttt tcgctattca
    34441 gcacccagac aaaatggcct tcattgccca tttgcagggc ggctgttggg atcactacgg
    34501 cgttttgttc ggtgtcgact aacatgcgcg cgttaacaaa ctgattggga aacagcgcat
    34561 catcctgatt attaaagcgt gctttcactt taatcgtacc ggttgtggca tcgatttggt
    34621 tatcgaggct taacagcgtg ccttcactta atttcttcga attggtgcga tcccaggctt
    34681 ctaccatcag cggttttccg gctttttgcg cctgcactac agtagcgata tcgctttccg
    34741 gcagggtaaa gactaaatcg ataggatgcg tctgggtgat caccacaatt ccggtggtat
    34801 caccactgga gatttggtta ccaacatcaa cttgcttgag accaacgcga ccatcgactg
    34861 gtgcggtgat gcggctccag tcgagttgca actgcgcgct ggcgacgctt gcttcatcag
    34921 ccttaatggt gccttcggtt tcactgacca gcgcctgttg ggcatccagc tcctgacggg
    34981 agacgagatt ggtttttgcc agttgctgat aacgcgccag atcacggcgg gcgttagcaa
    35041 gcgtggcttt atcttttgcc agttggccct gggtttgtgc taatgcaact ttgaactggc
    35101 tggggtcaat ttctgccagt aaatcgcctg ctttgacctg ctggccttcc tggaaatgta
    35161 acgccatcag ttgaccgtcc acgcggctgc gcaccgtaac agtattagcg gcggtaatgg
    35221 tgccaagccc ggt
//
LOCUS       DADLZM010000029        34756 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLZM010000029 DADLZM010000000
VERSION     DADLZM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.029"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(18..93)
                     /locus_tag="JNY73_001360"
     tRNA            complement(18..93)
                     /locus_tag="JNY73_001360"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JNY73_001361"
     tRNA            complement(152..228)
                     /locus_tag="JNY73_001361"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JNY73_001362"
     CDS             complement(331..1716)
                     /locus_tag="JNY73_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8047424.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JNY73_001363"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JNY73_001363"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY8047425.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JNY73_001364"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JNY73_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY8047426.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIFRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JNY73_001365"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JNY73_001365"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY8047427.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTWMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JNY73_001366"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JNY73_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY8047428.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /locus_tag="JNY73_001367"
     CDS             complement(6327..7457)
                     /locus_tag="JNY73_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY8047429.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JNY73_001368"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JNY73_001368"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY8047430.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8977)
                     /gene="rfbA"
                     /locus_tag="JNY73_001369"
     CDS             complement(8114..8977)
                     /gene="rfbA"
                     /locus_tag="JNY73_001369"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY8047431.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTDTHDSLIEASTFVQTVEKRQGFKIAWRNGWLDDEGVKRAASSLAKTGYGQYL
                     LELLRARPRQY"
     gene            complement(8996..10063)
                     /gene="rffG"
                     /locus_tag="JNY73_001370"
     CDS             complement(8996..10063)
                     /gene="rffG"
                     /locus_tag="JNY73_001370"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY8047432.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGHYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10063..11325)
                     /gene="wecC"
                     /locus_tag="JNY73_001371"
     CDS             complement(10063..11325)
                     /gene="wecC"
                     /locus_tag="JNY73_001371"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY8047433.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11322..12452)
                     /gene="wecB"
                     /locus_tag="JNY73_001372"
     CDS             complement(11322..12452)
                     /gene="wecB"
                     /locus_tag="JNY73_001372"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY8047434.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12508..13554)
                     /gene="wzzE"
                     /locus_tag="JNY73_001373"
     CDS             complement(12508..13554)
                     /gene="wzzE"
                     /locus_tag="JNY73_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY8047435.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13566..14669)
                     /gene="wecA"
                     /locus_tag="JNY73_001374"
     CDS             complement(13566..14669)
                     /gene="wecA"
                     /locus_tag="JNY73_001374"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY8047436.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14909..16168)
                     /gene="rho"
                     /locus_tag="JNY73_001375"
     CDS             complement(14909..16168)
                     /gene="rho"
                     /locus_tag="JNY73_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY8047437.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16253..16354)
                     /gene="rhoL"
                     /locus_tag="JNY73_001376"
     CDS             complement(16253..16354)
                     /gene="rhoL"
                     /locus_tag="JNY73_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY8047438.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16495..16824)
                     /gene="trxA"
                     /locus_tag="JNY73_001377"
     CDS             complement(16495..16824)
                     /gene="trxA"
                     /locus_tag="JNY73_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY8047439.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16955..18220
                     /gene="rhlB"
                     /locus_tag="JNY73_001378"
     CDS             16955..18220
                     /gene="rhlB"
                     /locus_tag="JNY73_001378"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY8047440.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18354..19838
                     /gene="gppA"
                     /locus_tag="JNY73_001379"
     CDS             18354..19838
                     /gene="gppA"
                     /locus_tag="JNY73_001379"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY8047441.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19885..21906)
                     /gene="rep"
                     /locus_tag="JNY73_001380"
     CDS             complement(19885..21906)
                     /gene="rep"
                     /locus_tag="JNY73_001380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY8047442.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            21993..22274
                     /gene="ppiC"
                     /locus_tag="JNY73_001381"
     CDS             21993..22274
                     /gene="ppiC"
                     /locus_tag="JNY73_001381"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY8047443.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22320..23795)
                     /gene="ilvC"
                     /locus_tag="JNY73_001382"
     CDS             complement(22320..23795)
                     /gene="ilvC"
                     /locus_tag="JNY73_001382"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY8047444.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMVDWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23945..24835
                     /gene="ilvY"
                     /locus_tag="JNY73_001383"
     CDS             23945..24835
                     /gene="ilvY"
                     /locus_tag="JNY73_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY8047445.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24832..26376)
                     /gene="ilvA"
                     /locus_tag="JNY73_001384"
     CDS             complement(24832..26376)
                     /gene="ilvA"
                     /locus_tag="JNY73_001384"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY8047446.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26379..28229)
                     /gene="ilvD"
                     /locus_tag="JNY73_001385"
     CDS             complement(26379..28229)
                     /gene="ilvD"
                     /locus_tag="JNY73_001385"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY8047447.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28294..29223)
                     /gene="ilvE"
                     /locus_tag="JNY73_001386"
     CDS             complement(28294..29223)
                     /gene="ilvE"
                     /locus_tag="JNY73_001386"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY8047448.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29243..29506)
                     /gene="ilvM"
                     /locus_tag="JNY73_001387"
     CDS             complement(29243..29506)
                     /gene="ilvM"
                     /locus_tag="JNY73_001387"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY8047449.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29503..31149)
                     /locus_tag="JNY73_001388"
     CDS             complement(29503..31149)
                     /locus_tag="JNY73_001388"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY8047450.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31152..31202)
                     /locus_tag="JNY73_001389"
     CDS             complement(31152..31202)
                     /locus_tag="JNY73_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047451.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31288..31386)
                     /gene="ilvL"
                     /locus_tag="JNY73_001390"
     CDS             complement(31288..31386)
                     /gene="ilvL"
                     /locus_tag="JNY73_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY8047452.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31738..33258
                     /locus_tag="JNY73_001391"
     CDS             31738..33258
                     /locus_tag="JNY73_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAY8047453.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33283..33621)
                     /gene="maoP"
                     /locus_tag="JNY73_001392"
     CDS             complement(33283..33621)
                     /gene="maoP"
                     /locus_tag="JNY73_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY8047454.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33740..34579
                     /gene="hdfR"
                     /locus_tag="JNY73_001393"
     CDS             33740..34579
                     /gene="hdfR"
                     /locus_tag="JNY73_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY8047455.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLSGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGSLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34675..34750)
                     /locus_tag="JNY73_001394"
     tRNA            complement(34675..34750)
                     /locus_tag="JNY73_001394"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34715..34717),aa:Trp,seq:cca)
BASE COUNT         8477 a         9507 c         8777 g         7995 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt atgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gaaaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccacgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcacgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcataa
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctta aagccctggc gtttttctac tgtctgcaca aaagtgctgg
     8281 cctcaatcag gctgtcgtga gtgtcggtat ccagccaggc aaatccgcgc ccgagcagtt
     8341 caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc tccagttcac
     8401 cgcgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg tagaaataaa
     8461 gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttcaagc gagatagcgc
     8521 ggaaattgtc gtcaaattcc accacgccaa agcgttccgg gtccatcacc tgatagtcaa
     8581 aaaccgtcgc cccttccgtg cgcgccgcaa catgacgcag cttcggactg aagccctgac
     8641 cgaagaagat gttatcgccc agcaccagac aagaaggttc accattaagg aaggtttcac
     8701 cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg ttcggcatat tccagctgga
     8761 taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct tccggcgtag
     8821 tgatgatgag aatttcgcgg ataccggcca gcatcagcac cgacagcgga tagtaaatca
     8881 ttggcttatc gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc
     8941 gggtaccgga accgcccgcc aggataatac ccttcatgtt tgcctccgct gaaaattagc
     9001 ctttcagacc taaacgctca ccctgatagc tgccgtcctg cacctgcttc caccagcttt
     9061 cattagccag ataccactga accgttttac gcattccact ttcaaaggtt tcctgcggca
     9121 gccagccaag ttcacgggca attttcgaag catcaatggc ataacgcaga tcatgccccg
     9181 gacggtcagc gacaaaggtg atcaggtcac gatagtgcgc cacgccgtgc ggcttgttcg
     9241 aagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc ttacgctcgt
     9301 tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg caatacagcg
     9361 cgcgggcgtg atcttccaca tacagccagt cacggatttg ctgcccgttg ccatataccg
     9421 gcagcggttt acccgccagc gcgttgagga tcatcagcgg gatcagtttt tccggaaagt
     9481 ggtaatgacc gtagttattc gagcagttgg tgatcagcgt cggcagaccg taggtccgca
     9541 gccaggcgcg caccagatgg tcgctgctgg cttttgacgc ggaataaggg ctgctcggcg
     9601 catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca tatacttcgt
     9661 cggtggagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc gtattccagt
     9721 aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca ataaacgctg
     9781 ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg acacagtctg
     9841 gttgatgctc agtgaatacg cgtgccagtt ctgcccgatc gcagatatca actttctcaa
     9901 aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat caggtttccg gcgtaggtca
     9961 gcttatcgac cactaccacc gcatcgctcg tttcgttgat gatataacgc accagcgccg
    10021 agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc agactccttt
    10081 ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttga actgactatg
    10141 atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt
    10201 acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa ccagcgtttc
    10261 gccgctgtgc cactgggcga tcagttcagc gatttccatc gccgggcttt cgcgcaggtc
    10321 atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt
    10381 atcggtagca gcaaggcagt cggcaaccgc cgctttcacc tgatcgataa cccagaacgg
    10441 tttgtgatcg ttaacttcgc gcgcggtacg gataagccgc gcctgctggg ggttctgtgc
    10501 cacgataaac cacggatcaa cagcaatgca gtgaccgccc acgccagggc caggctgaag
    10561 aatattaacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt taatcccctg
    10621 atcggcacaa atcagcgaca attcattagc aaaagcgata ttcacatcgc ggaagctgtt
    10681 ttcggtgagc ttacacattt ccgccgtccg cgagttagtg acgacacact caccttcgag
    10741 gaaaattttg tacaattcgc tggcgcgggc cgaacaaacc ggcgtcatac caccaatcac
    10801 gcgatcgttt ttaatcagct cgaccataac ctggcccggt aatacacgtt ccgggcagta
    10861 agcaatgttg acgtccgcct gctcgcccac ctgctgtggg aaagtcagat ccgggcgcat
    10921 ctctgctaac cattcagcca tcttctcggt tgaccccacc ggcgaggtgg attcaaggat
    10981 caccagggcg ccttttttca gcactggcgc aatggagcga gcagccgatt caacgtaggt
    11041 catatctggc tcatgatcgc ctttaaacgg cgtaggtaca gcaatcagcc aggcatccgc
    11101 ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt ttactacgct
    11161 cgccaaatca ggctcgacga tatggatttc gccacgattg atggtatcaa ccgcgtgttg
    11221 gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag
    11281 cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga tatccgatta
    11341 ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg gttatgggcg
    11401 cggctcatag cttgatattc gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt
    11461 cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc cggacgctca
    11521 gtggtatcgc gcatcaccag caccggtttc cccagcgaag gcgcttcttc ctgaatgccg
    11581 cctgagtcag tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc
    11641 tggggatcga tcagaatgac atttttcaca tgccccagaa tgcgattgac cggctctctg
    11701 acgttcgggt tgagatgcac cggatagaca atctggatgt cctggtgcgt ggtggcgatg
    11761 tctgccagcg cgtggcagat ttcttcaaag ccacgaccga aactctcacg cctatgaccg
    11821 gtcaccagaa tcatcttttt atcgggggcg ataaacgggt aatttgccgc cagttctgaa
    11881 cgcagcgtgt cgctgctcat cacctggtca cgcacccata acagtgcatc aatgactgta
    11941 ttaccggtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt ttgccgggaa
    12001 gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg gttagcctct
    12061 tccggccacg gcgaatagag atcgcccgtg cgcagaccag cctcaacgtg accaacagga
    12121 atacgctgat aaaacgccgc caggctggtt gccagcgtcg tcgtcgtatc gccgtgaacc
    12181 agcacaacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag aatccgacag
    12241 gttatctctg tcaggccctg tcctggctgc attatgttga gatcgtagtc aggtacaatg
    12301 gaaaagagtt tcagtacctg atcaagcatc tcccgatgct gcgcagtgac gcaaacttta
    12361 gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gagccatctt gatggcttcc
    12421 gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata agcggcgagc
    12481 gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta atgcgacacc
    12541 agccccgatc agccccccga caatgcccca cataatcatc aggaaggcac gacgtgggct
    12601 atcgcgtttt accggttctt ccggcgtacg caaatagcga taggtctgaa aacgcggatc
    12661 cagggttgga ccaacattca gggtgtttaa catggctcga ttctgatcat agtcgagatc
    12721 aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg gacgcccaag
    12781 caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat
    12841 attatgctgc tcagcaattt tcagcgcctg ctcaatgctg ttcatccggc ggtcgtagat
    12901 ggctttcgcc acctcttcct gacgcttcac ctgagctttc atctggatgg tgcgtgccgc
    12961 ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg caaaagcaac
    13021 atactgacgt aacaggttat tagcgtcagg cgcggtttcg gcaataagct tcacgctgtc
    13081 attgaccgcg cgagtaaagt ctccggggat aaactggatg ttgttaatca tttcatccag
    13141 caacgccgca tcggctttgc tgttgcccac catccgctgt ttgtaatagt cggtttgcag
    13201 ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt tataggcttc
    13261 gtccatgacc gatggttgat cggcagaagc catgtttgaa cggacatcca ggttacgcaa
    13321 aaattgctgc tgcgagtaat atccccccag catattcacc gttggacgat cggtaatcgc
    13381 cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc agcgcgatta acgcaaacgc
    13441 cagccccatg ccaataatcc atagcttccc agcccacaag gtacgaaaca acccacgaat
    13501 atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg tcatcacatc
    13561 ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct gcgttttacg
    13621 cgcttaataa agcgagcaac tttccaggca cgcttaatgc aatatccata gaggaagaat
    13681 gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata ttctgccagc
    13741 acgccaatgg aagcgagcag tgctgcggca agggtaatca gcacaaacgc ctgacgggaa
    13801 gtaaacccgg cacgcatgat caaatggtga atatgctggc ggtcaggaga gaatgggctc
    13861 atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg aatggcgatt
    13921 atccacaaag cggtaaccgg gctgatggga tgggttttgc cctgggtcgt ttcgagcagg
    13981 atccagataa cggtaaaacc aatcagcgta ctgcccgcat cacccataaa gactttgtag
    14041 cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc gatcatcgca
    14101 aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc gattgctgca
    14161 aacgagacgc aggacaaccc gcccagcaag ccatcaatgc catcaaccat gttgaacgca
    14221 ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc gagcaccatc
    14281 tcccaggagc caaagatata acccaggcta ctgagataaa gtttgccgaa caccatcata
    14341 acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc aaaacgatca
    14401 tccagcgcgc caatgaaaac aagcacaccg gcacaagcga gatagagaga tgcatgcgga
    14461 atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga aatcccccca
    14521 acgagaggta tcaatccctg gtgacgtttg cggaagtttg gtttatccac taaaccgact
    14581 ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg
    14641 agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa ttattagcag
    14701 aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc caccagccct
    14761 gtgcaaaaat taaccatttg atatgcaaat tgcataacat gccagcaaat tccaggaatt
    14821 ttcctaacat cgccagcgcg acattaagat tacagatata aaagcaaaac gccacgtaaa
    14881 cacgtggcgt ttttggcata agaccaattt atgagcgttt catcatttcg aagaaatcgt
    14941 cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg atttcgccca
    15001 tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga gtcgtgagca
    15061 gctcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg cgtttttcag
    15121 cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtaaataa
    15181 cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatggtc aggctgccgc
    15241 cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgaagg gcattggcgt
    15301 ccacaccacc ggtcaacact ttacctgacg ccggaacaac agtgttgtaa gcgcgcgcca
    15361 gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc aggcgtttgg
    15421 ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag
    15481 aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt
    15541 cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc tgagcaatgt
    15601 tctgcagcag catagtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac
    15661 gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta gaaccgttac
    15721 cacgttccat acgcagacga gagtttgcgt gcagcggggt taagttctca aagaggattt
    15781 tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat
    15841 agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt
    15901 tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc
    15961 tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg ccatcaccaa
    16021 agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac
    16081 gcatgcgagc caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg
    16141 gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc
    16201 tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac
    16261 tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat
    16321 tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa
    16381 attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta agttagcatg
    16441 actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat tcccttacgc
    16501 caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca ctttggttgc
    16561 cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc catatttcgg
    16621 cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc
    16681 gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca
    16741 gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt
    16801 caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg cgtgttggtt
    16861 tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg atacgctttc
    16921 gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt taacagaaca
    16981 gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt
    17041 tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg gtcgtgacgt
    17101 agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt caacgtttca
    17161 ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc gtgccttaat
    17221 tatggcaccg acgcgtgaac ttgccgtgca gatccacgcc gacgcggaac cgctggcgga
    17281 agctaccggc ctgaagctgg gcctggctta cggtggtgat ggctatgaca aacagctgaa
    17341 agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa ttgactacgc
    17401 caagcagaac cacattaacc tcggtgccat tcaggtagtg gtactggacg aagccgatcg
    17461 catgtacgat ctaggcttta ttaaagatat ccgctggctg ttccgccgta tgccgcctgc
    17521 aaatcagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac gtgaactggc
    17581 gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga aaacgggcca
    17641 ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac
    17701 gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca aacaccgttg
    17761 tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga
    17821 tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg atctggatat
    17881 tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga cgcacgtctt
    17941 taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc gtacaggtcg
    18001 cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat tgaatttgcc
    18061 tgctattgag acctatattg gtcactcaat tccggtaagc aaatacaatc cggacgcatt
    18121 gatgaccgat ctgccaaaac cgctgcgcct cacgcgcccg cgcacaggca atggtccgcg
    18181 tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa
    18241 tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcttac tgtagtaagt
    18301 gattcgggtg aaagaatgca gccaacacag agacagattg aaggatgaag agtatgggtt
    18361 ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg
    18421 tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat taaacgcaaa gtgcgtctgg
    18481 ctgctggcct gaatagcgaa aatgacctct ctaatgaagc aatggagcgc ggttggcaat
    18541 gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg
    18601 ctacggcgac attacgactt gccgtcaatg cgggtgattt tattgccaaa gcgcaggaaa
    18661 tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcgcgtctg atttatcagg
    18721 gcgttgctca caccactggt ggtgccgatc agcgcctggt ggtggatata ggcggtgcca
    18781 gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc ctgtcgatgg
    18841 gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcaa gaaaattttg
    18901 atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc
    18961 acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag gaaatcatga
    19021 tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg aaacagcgag
    19081 ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg gaacgtgcgt
    19141 tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg aatattcagt
    19201 gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg ttgcatctgg
    19261 ccgtcgagca ggatattcgc agccgtacac tgcgtaatat tcaacgccgc tttatgatcg
    19321 atattgatca ggcacagcgc gtagccaaag ttgcggctaa cttcttcgat caggtggaaa
    19381 acgaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg
    19441 aaatcggcct gagcgttgac ttcaaacaag ctccgcaaca cgctgcttat ctggtgcgta
    19501 acctggatct tcccggtttt acccccgcac agaagaaact gctggcgacg ctactgctca
    19561 accagactaa cccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccaccgcgcg
    19621 tcgcagaaca actctgccgt ttactccgcc tggctatcat ttttgccagc cgtcgccgtg
    19681 acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc
    19741 cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag gaaaaccagt
    19801 ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg
    19861 ctgacgcatc ttctccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag
    19921 attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcactgacca ctttgcgctc
    19981 ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg gctccgggcg
    20041 caccagttcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc
    20101 gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt catcgatact
    20161 gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag gaaactccag
    20221 cccttttgac gcatggagag tcatcagttg cacctgatcc agctcttctt cactctcacc
    20281 acgctccatc atgtcgcgta aagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc
    20341 atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt
    20401 catgcgcatt tcggcagctt tcgggctggg cgatgtttcg tacagccagg attcataatc
    20461 catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca gacgctggat
    20521 ttctgccaac cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg
    20581 actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccactc
    20641 acccagcttt ttcagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa cgatacgcag
    20701 aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat
    20761 ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc ggttttgcat
    20821 caggaatttt tcaaacaccc gcgactgatg gttaccgcga taaagaatag cgtaatcttt
    20881 gtactgcgtt ttattgacga agtgatgcgc aatcagttca ccagtgacgc gctcggcttc
    20941 atgttcttcg ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca gttcggagaa
    21001 cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat
    21061 acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg
    21121 gctcagcagc accaggtttt gcggacgtgc gccgcgccag gagtagatcg actggtcatc
    21181 gtcacccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca gttcatactg
    21241 gctggtgttg gtgtcctgat attcgtccac cagcaggtag cgaattttgt tctgccagcg
    21301 ctcgcggact tcttcattgc gttgcagaag tagcgtcggc aataaaatca gatcatcgaa
    21361 gtcgagcaca ttacaggctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat
    21421 acggtcccgc tcgccaatcg cactggctgc cgcctgggac ggtgttttga gatcgttctt
    21481 ccagttagag atggtcgaaa tcaactgttg caggagaact ttgtcatctt caatcagccc
    21541 ttcggtcaac tctttgagca acgccagctg atcggtatcg tcaaacagcg aaaagttcgc
    21601 cttcatcccc agcgccgcat actcacgctt gatgatatcc agccccagcg tgtggaaggt
    21661 tgagatcatc agcccacgcg cctctttgcg acccagcgtc tgtccgacac gctctttcat
    21721 ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct gataaccgca
    21781 cccgcggatc agatgggcga ttttattggt gataacacga gttttaccgg aacccgcacc
    21841 cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag
    21901 acgcatagga aaatcgctca atgaaagtcg ggaggggaaa ataacagcgt ggtagtatag
    21961 ccagcctcat ccttaccact caaggcacga tcatggcaaa aacagcagca gcactgcata
    22021 tccttgtaaa agaagagaaa ctggctctgg atcttctcgt gcagattaag aacggtgccg
    22081 atttcggcaa gctggcgaag aaacactcca tttgcccatc aggcaaacgc ggcggtgatt
    22141 taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagttgtg ttctcttgtc
    22201 cggtactgga gccaaccggc ccgctgcaca cccagttcgg ttaccacatc attaaggtgc
    22261 tgtaccgtaa ctaatagcaa ggccttctcc gggagaaggc cttgaactct gaatcgctat
    22321 taacccgcaa cagcaatacg tttcatatcc gtcatatagc cgcgcagttt cttacctact
    22381 tgctcaatcg catggctgcg aatcgcttca tttacatcac gcagctgcgc gttatctacc
    22441 gcaccttccg gaatagcttt acccaaatcg cccggttgca gttctgccat aaacggtttc
    22501 agcagcggca cacaagcgta agagaacaga tagttaccgt actcggcagt atcagagata
    22561 accacgttca tttcatacag acgcttacgg gcgatggtgt tggcaatcag cggcagttcg
    22621 tgcagtgatt catagtaagc agattcttcg atgatgccgg aatcaaccat ggtttcgaac
    22681 gccagttcaa cgcccgcttt caccatcgcg atcatcagta cgcctttatc gaagtactcc
    22741 tgctcgccga ttttgccttc atactgcggc gcggtttcga aggcggtttt gccggtctct
    22801 tcacgccagg tcagcagttt cttatcatcg ttggcccagt ccaccatcat accggaggag
    22861 aattctccgg agatgatgtc gtccatatgt ttctggaaca gcggtgccat gatctctttc
    22921 agctgttcag acaacgcgta agcacgcagt ttcgccgggt tagagagacg gtccatcatc
    22981 agtgtgatac cgccctgctt cagcgcttcg gtgatggttt cccaaccgaa ctgaattagt
    23041 ttttctgcgt atgccggatc ggtgccctct tccaccagct tgtcgaagca cagcagcgag
    23101 ccagcctgca acataccgca caggatggtt tgctcaccca tcaggtcaga tttcacttcc
    23161 gcaacgaagg acgattccag cacgcccgca cggtgaccac cggttgctgc cgcccatgct
    23221 ttagcgatcg ccatgccttc gcctttcgga tcgttttccg ggtgaacggc aatcagcgtc
    23281 ggtacgccga atccacgttt gtactcttca cgcacttcgg tgccagggca tttcggcgca
    23341 accatgacta cggtgatgtc tttacggatc tgctcgccca cttcgacgat gttgaaaccg
    23401 tgcgagtaac ccagcgccgc gccgtctttc atcagtggct gtacggtgcg cactacatca
    23461 gagtgctgct tgtccggcgt caggttaacc accagatccg cctgtgggat cagttcttcg
    23521 taagtaccca ctttaaaacc attttcggtc gctttacgcc aggacgcgcg cttctcggca
    23581 atcgcttctt tacgcagagc gtaggagata tcgagaccag aatcacgcat gttcagaccc
    23641 tggttcagac cttgtgcgcc acagccgacg atgactactt ttttaccctg aaggtagctc
    23701 gcgccatcgg cgaattcatc gcgccccata aagcgacatt tgcccagctg tgccagctgc
    23761 tgacgcagat tcagtgtatt gaagtagtta gccatggtga ttcctcgtga tgttgtgctt
    23821 cttattgttc ggttcgcgtt tgcgagattg aattcactat atgacaggaa atttattgcg
    23881 gaaattgata tattcacaac gtcacattgc aatttctgca acgtcaacat tgagggctgc
    23941 gccattggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc gccattttgg
    24001 ccgcagcgcg cgggcgatgc acgttagccc gtccacgctc tcacggcaga ttcagcgcct
    24061 ggaagaagat ctcggccagc cgctgtttgt gcgcgataac cgcacggtga cgctgactga
    24121 agcgggcgaa gaactgcgcg ttttcgccca gcaaacgctg ttgcagtatc agcagctgcg
    24181 ccacaccatc gaccagcaag ggccgttgct ctatggcgaa ttacatatct tctgctcggt
    24241 aaccgccgct tacagccatc tgccgccgat tctggatcgc ttccgcgcgg aacacccgtc
    24301 ggtggagatc aaactcacta ccggtgatgc ggcagatgca atggaaaaag tcgtcacagg
    24361 tgaagctgat ctggcgattg ccgggaagcc agagactctg cccggcgcgg tggcgttttc
    24421 gatgctggag aatctggcag tggtgttgat tgcacccgca ttgccctgcc cggtgcgtaa
    24481 tcaggtttct gtcgaaaagc cggactggtc aacggtaccg tttatcatgg ccgatcaggg
    24541 gccggtgcgc cgccgcattg agttgtggtt ccgacgcaat aaaatcagta acccgatgat
    24601 ttacgccacg gttggcgggc atgaagcgat ggtatcgatg gtggcgctcg gctgtggcgt
    24661 ggcattgttg ccggaagtgg tgctggaaaa cagccccgaa ccggtgcgta accgcgtgat
    24721 gattttagag cgcagcgatg agaaaacgcc gtttgagctg ggcgtgtgtg cgcagaaaaa
    24781 gcggctacat gagccgctaa ttgaggcatt ctggaagatt ttgccgaacc attaacccgc
    24841 caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc ccagctcatt
    24901 cagccgggtt tcgaaatccg gttcatggtc gccaagttcg aacgccgcca gtacgcgccc
    24961 gtagtcggtg ccatggctgc gatagtggaa caaagaaatg ttccagtgcg tacccagcgt
    25021 gttgaggaag cgcagcagcg cgcccggtga ttccgggaat tcgaagctgt agaggcgttc
    25081 ctgcaacgga tgtgacggac gcccgccgac catatagcgc acatgcaact tcgccatttc
    25141 gtcgtcggag agatccacca cgctgtagcc gccgtcgttg agcatctgca aaatttcttt
    25201 gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag atgcaggcgt ttttggcatc
    25261 ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca agcagttggc agaatttgag
    25321 gaagctgcct ttttcttccg gaatggtcac cgccaacaac gcttcacgct gttcgcccag
    25381 ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc acgttggcac cggaaagaat
    25441 atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata tattttttca ttcccgccag
    25501 cgccagcgcg ccagagggtt ccgccaccgc acgcacatct tcgaataaat ccttcatcgc
    25561 cgcacagatc gcatcgctat cgacggtgat gatgtcgtcg agatactcct ggcataaacg
    25621 gaaggtttcg tcaccgatgc gttttaccgc gacaccttcg gcgaacagtc ccacgcgtgg
    25681 cagatcaacc ggatgacccg catccagcgc tgctttcagg caggcggagt cttccgcttc
    25741 tacggcgatc actttgattt gcggcatcag ttgtttgatc agcaccgcta cgcccgccgc
    25801 cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggagtcct gctggagcag
    25861 ttccagcgcc agcgtgcctt gcccggcaat caccatcgga tggtcgaacg gcggcaccca
    25921 ggtgaacccc tgctgctgtg acagttcgat cgctttagct ttcgcttcgt caaagttcgc
    25981 gccatgaagc agcacttcgc cgccgaatcc acgtaccgca tcgactttga tgtcggcggt
    26041 ggcggttggc ataacgatca gggccttcac gcctaaccgc gcagaagaaa acgcgacgcc
    26101 ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc gctttttgtt cttccgtcag
    26161 gcccgccatc atggcgtatg cgccgcgcag cttaaagctg tgcactggct ggcgatcttc
    26221 gcgcttcacc agaatgacgt tatcaagacg cgacgacagt ttttccattt tttgtagcgg
    26281 cgtaacctgc gccgcctcgt aaaccggcgc gcgcagcact gctcttaaat attcggcacc
    26341 ttccggagca ccggacaggg gttgcgagtc agccattatt aaccccccag tttcgattta
    26401 tcgcgcaccg cacctttgtc ggcgctggtt gccaggctgg cgtaggcgcg cagggcaaag
    26461 gaaacctgac gttcacggtt tttcggcgtc caggctttgt caccccgagc ttcctgcgct
    26521 tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg gttcgggatg
    26581 tcgatagcga tcaggtcacc atcttcaatc agaccaatgc tgccgccgct tgccgcttcc
    26641 ggtgagacgt ggccgatgga aagaccagag gtgccgccag agaaacgacc gtcggtgatc
    26701 agcgcacagg ctttaccgag acccattgat ttcaggaagc tggttgggta gagcatttcc
    26761 tgcatgccag ggccgccttt cgggccttca tagcggatca ccaccacatc gcccgcgaca
    26821 actttgccac cgaggatcgc ttctaccgcg tcgtcctggc tttcgtacac tttcgccggg
    26881 ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt tcacgatgca gccgttttca
    26941 gcaaaattac cgtagagcac cgccaggccg ccgtctttgc tgtaggcgtg ttccagcgag
    27001 cggatacagc cgtttgcgcg atcgtcatcg agagaatccc aacggcaatc ctgcgagaat
    27061 gcctgtgtag tacgaatgcc cgcagggcct gcgcggaaca tattttttac cgcgtcatcc
    27121 tgggtcagca taacgtcgta ttgttccaga gtttgcggca acgtcaggcc aagtacgttt
    27181 ttcacatcac ggttcagtaa ccccgcgcga tccagttcac cgagaatacc gataacacca
    27241 ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcaac tttgcataac
    27301 tgcgggactt tacgcgaaag tttgtcgata tcactcatgg tgaagtcgat ttccgcttcc
    27361 tgcgccgccg ccagcaggtg aagtaccgtg ttggtagagc cgcccatcgc gatatccagc
    27421 gtcatggcat tttcaaacgc cgctttgcta gcaatattgc gcggcagtgc actttcgtcg
    27481 ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt taccggcatt gaggaacaac
    27541 tgcttacggt ccgcgtgggt tgccagcagc gagccgttgc ccggctgcga caggcccagc
    27601 gcttcggtca ggcagttcat tgagttagcg gtaaacatcc cggagcagga gccgcaggtc
    27661 ggacacgcgg aacgttcaac ctgatcgctc tgggagtcag aaactttcgg gtctgcgccc
    27721 tggatcatcg catcaaccag atcgagtttg attatctgat cggacagttt ggttttcccg
    27781 gcctccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag ggaagccatc
    27841 agcatccccg gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc ggcgcagtgg
    27901 gcgttgacca tatactcaac ggaatcagcg atcagttcgc gagatggaag tgaataaagc
    27961 atccccccgt ggcccatggc aatcccatca tccaccgcaa tggtgttgaa ctctttggca
    28021 acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg cagatggacg
    28081 tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt gccaaaatcg
    28141 gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt acgaccatga
    28201 gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc tgtctcgtaa
    28261 atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat ctaaccagcc
    28321 ccatttatct tcggtttcgc cagtgaagag gccgaagaag gcttgctgaa tgcgtttggt
    28381 aaccgggcca caacggcctt cgccaatctg aataccgtct acgctgcgca ctggcgtgat
    28441 ttctgccgcc gtaccggaca taaagacttc atccgccaag tacagggatt cgcgcgacag
    28501 cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg catcacgggt
    28561 aatacccggc agcgcggagg aggtgaacgg tggggtgaac agcacaccat ctttcacttc
    28621 aaacaggttt tcgcctgcgc cttcagagat ataaccgttc acatccagcg cgataccttc
    28681 ctgataaccg tggcggcgcg cttcgctacc caccagcagg gaagagagat agttaccacc
    28741 ggcttttgcc gcagttggga tggtgtttgg tgctgcgcgg ttccaggagg aaaccatcgc
    28801 atcgatcccc tgctccagcg cttctgcgcc cagatacgct ccccacggga aagcggcgat
    28861 aatcacatcg gttgagtatc ccgctggcgg gtttactccc atgccaacat caccgacgaa
    28921 gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt cacgacaagc
    28981 ttccatcagc tcatcaatgc tctgtgaaac cgggaagcga tagattttgg cggagtcatg
    29041 cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgtg agtcgtagca
    29101 acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca catgcacctt
    29161 cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt tcttcgtggt
    29221 catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt gtgctctggc
    29281 agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt aagtcgaccg
    29341 accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg gcggcagcca
    29401 tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca cgttctaagg
    29461 tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata atttctccaa
    29521 catttctgaa ttactggcac ctggcggcac cagcggccag acgttctcaa gttcgtcgat
    29581 tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg ccgcttcaac
    29641 ctggtcttta cgggtaatgt gttggccagg gatgccgaag gcgctggcta acatgaggaa
    29701 atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tgaaaaaaca gttgctgcca
    29761 ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg gtaactgctt
    29821 gcgttttacg gtgcccagct cttgcacatt catcatgaaa gagccgtcac cggagataca
    29881 gacaacggta tcgttcggtc gcgcgacttg tgcgccaact gccgccggta aaccaaaacc
    29941 catggtacct aagccgctgg aggtgatgaa attttccggg cgagtgtggg cgatgtgctg
    30001 cgcagcccac atctggtgct gccccacatc tgtggtcacg acgcaatccg caggtttacg
    30061 atccgacagt tgttttaaca acaacggagc gtagatagcg tcaccgggat ggtcgtaacg
    30121 ccaggaatgt tcatcacgca gctgcgcgca gtgttgctgc cagtcattga tatttaacgg
    30181 ctgctgtaat gctggtaaca gagcatttaa atcaccttgt aatgccacat gtgcctgacg
    30241 cagcttgttc atttctgccg ggtcgatatc catatggata acactggcgt gtggtgcgaa
    30301 ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca cccacggcga tcagcaggtc
    30361 acactcctgc accgcgaagt ttgccgcttt ggttccgtgc atccccagca tgcccagata
    30421 gtacggataa tctgcttcta ctgcgcccag ccctttcagc gtacaggtgg caggcatttt
    30481 tgtggcagcg agaaattcac gcaaagccgg aactgcctgc gccatcccca cgccaccgcc
    30541 aacgtacagc atcggttttt gcgcttttgc cagcatctgg cgcgcttgct cgacttcggc
    30601 atgtgggaaa gtcacttcgt tttcaacggt ggtgaaccac ggttccagat cgccgctggc
    30661 taactggata tcttttggga tatcgaccag aaccggacca ggacgacctg agctggcaac
    30721 gtcgaatgct tcagccatga tgcgcggcaa ctcttccaga gactgcacca gaaagctgtg
    30781 cttggtacag gctaacgaca atcccaggac atccacttcc tgaaatgcgt cggtgccgat
    30841 aaacggtgcg gacacctgac cagtgatggc aacgacaggg atggaatcta acagtgcgtc
    30901 cgcaagcccg gttatcaggt tggttgcgcc cggaccagac gtggcgatac atacgccagt
    30961 tttaccggta gcacgggcat aaccgatagc cgctattgcc gcaccctgct catgtcggca
    31021 cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa accggcataa ttgcgccacc
    31081 cggataaccg aaaacggtgt tcacaccctg tgcccgcaac gcatgtacca cccactgtgc
    31141 gccattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt gctgttattc
    31201 attgtctgct cctcggttat gtttttaagg tcaaaaaaac ccccggacct ttcggtgcgg
    31261 gggtcttagt tcgttaaggc ttgatctcta agcctttcct cgtccaagtg cagccccgca
    31321 cggtgggata ataatcacca ccacgctaat cacgaccagg ctaatcactc gtagaagggc
    31381 tgtcattttg tcttttcttg catcttgttc gaaggaatgc ctaaagagtt accatagatt
    31441 ttgcgaatag cacaagatat ttttgggcct atttttatga tggctttaat aatttcaata
    31501 gaaattgttg ttttttaaaa agaaaatagt gagtgaaaaa attcaatttc agtataaatg
    31561 ttgttccctt cctcgtagtt catcctttgt ccgctgcatt tttgcgagca tcattccacc
    31621 gccagaacaa ctcaacttca cttgcacaaa taaacaggaa tcagccacta aaattcacga
    31681 attagcgggc tacagcagcg atatcttatg cacataatgg tgactcaagg agggtttatg
    31741 tcactgtcaa ttgttcatac ccgcgcagcc ctggaagtaa atgcgccccc aatcactgtt
    31801 gaggtacata tcagtaaagg tctacccggc ttaacgatgg tgggcttaac agaaacaacg
    31861 gtgaaagaag ctcgcgatcg cgtgcgcagc gccattatca atagcggata tgaatatccg
    31921 gcgaaaaaaa tcaccatcaa ccttgctcca gccgatctgc caaaagaagg gggacgatat
    31981 gatttaccta tcgccattgc gttgctggcg gcctcagaac agcttacagc caataagtta
    32041 gatgaatatg aattagtcgg agaactagcg cttacaggcg ctctgcgtgg cgttcccggt
    32101 gcaatctcca gtgcaactga agctattaag tcgggcagaa aaattatcgt cgcgaaagat
    32161 aacgaagatg aagtggggct aattaacggt gaaggatgcc tgatagccga tcatctgcag
    32221 gctgtctgtg cgtttctgga aggtaagaac actctcgaac gcccgaaacc aactgatgca
    32281 gtatcccggg cgctacaaca tgatctcagt gatgttgtcg gtcaggagca aggaaaacga
    32341 ggactggaaa ttaccgccgc tggtgggcac aaccttttac tgattgggcc gccgggaaca
    32401 ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc cagatttaag caatgaagag
    32461 gcactggaga gcgctgcgat attaagtctg gtaaatgctg aatcagtaca aaaacaatgg
    32521 cggcagcgcc cgttccgctc acctcatcac agtgcgtcgt taactgcgat ggtgggcggt
    32581 ggtgcaattc cagggccagg tgaaatttcg ttggcgcata acggcgtgct ttttcttgat
    32641 gagctacctg aatttgaacg gcgtacactg gatgccttgc gagagccgat tgaatccggg
    32701 cagatccatc tttcacgcac acgagcaaaa ataacctatc cagcccgttt ccagcttgtt
    32761 gcggcgatga atcccagccc taccggacat tatcagggaa accataaccg ctgcacgcca
    32821 gaacagacat tacgttatct caaccggctc tcggggccct ttctcgaccg cttcgatctc
    32881 tcactggaga tcccattacc accccccggc attttgagta aaacggtagt gccgggagaa
    32941 agcagcgcca ccgttaaaca acgcgtaatg gccgccagag agcgccaatt taagcggcag
    33001 aataaactga atgcctggct ggatagtccg gaaatacgcc aattctgcaa acttgagagc
    33061 gaagatgcga tgtggttgga agaaacgctg atccatctgg ggttatcgat tcgtgcctgg
    33121 cagcggttac tgaaagttgc acgaaccatt gctgatattg atcagtctga cattatcaca
    33181 cgtcagcatt tgcaggaggc agttagctat cgtgcgattg accgtttgct catccatctg
    33241 caaaaactac tgacataaaa aaagggcatt tcgccctttt tattaatcgt cagaatcggt
    33301 gtagtcttca gcaccttcaa cctgcggttt accgccggaa agggtgtgaa aacgttttgg
    33361 acgcttaata cgcgtcatat acttggacca cacgcgttct gcttctgtca ctggctcacg
    33421 ttcgccacgg catactgcta cgaagagttt ctcttcctcg gtaaccggct cgcgtttgcc
    33481 aagatccaac tcattgaagg cataaccatg acgctcaagc agttgtgcct ctttgatggt
    33541 gaaatcacca tgacgagaga atccacgtgg ataatgttta ttgtcgaaat atcgattagt
    33601 cgtcgtaaag ctttccgcca tcctgcacgc tcctaattct ttgaccgagc tagttatggc
    33661 gcggagtatt agttacgctt gacagagtgt aaaacaaaac atttaaatca taacgacaaa
    33721 taattttgcg gagagcactg tggatacgga attgttaaaa actttcctgg aagttagccg
    33781 aacgcgtcac tttggtcgag cggctgaatc gctctatctg acccagtcag cagtgagctt
    33841 tcgaatcaga caactggaaa atcaactggg tgtgaacctt ttcacccgcc acagaaacaa
    33901 tatccgttta accgctgccg gtgaaaaact actgccttat gcagaaacgc tcatgagcac
    33961 ctggcaggcc gcccgcaagg aggtggcgca tacctcacga cataacgagt tttctatcgg
    34021 tgccagcgcc tcgttgtggg aatgtatgct taatcagtgg ttgggacgct tgtatcaaaa
    34081 tcaggatgcc catacaggct tacagttcga agcgcgaatt gcccaacggc agtctctggt
    34141 aaaacagctg catgaacgcc agcttgatct tcttattacc actgaagcgc ccaaaatgga
    34201 cgaatttagt agtcagttgc tggggtattt cactttagcg ctttatacca gtgccccttc
    34261 taaactaaag ggagatctta actatctgcg actggagtgg gggccagatt ttcaacagca
    34321 tgaggcaggt ttgagcggtg ctgacgaagt tcccattctg acaaccagtt ctgctgaact
    34381 ggcacagcaa cagattgcga tgcttaatgg ttgcacctgg ctacccgtca gctgggcgcg
    34441 taaaaaaggc agcctgcata ccgttgtcga tagcacaaca ctttcacggc cgctttatgc
    34501 catatggctg caaaatagcg ataaaaatgc gttgattcgc gatcttttga aaattaacgt
    34561 gctggatgaa gtgtattaat ctgaatggct ggcaaggatg ccggtagaag gatttacttc
    34621 ggagaggatt atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggcag
    34681 gggcggagag actcgaactc ccaacacccg gttttggaga ccggtgctct accaattgaa
    34741 ctacgcccct aattag
//
LOCUS       DADLZM010000030        34281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLZM010000030 DADLZM010000000
VERSION     DADLZM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.030"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(93..2189)
                     /locus_tag="JNY73_001395"
     CDS             complement(93..2189)
                     /locus_tag="JNY73_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8047456.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYARELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2191..2442)
                     /locus_tag="JNY73_001396"
     CDS             complement(2191..2442)
                     /locus_tag="JNY73_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8047457.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2595..3158
                     /locus_tag="JNY73_001397"
     CDS             2595..3158
                     /locus_tag="JNY73_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY8047458.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3434..3856)
                     /locus_tag="JNY73_001398"
                     /pseudo
     CDS             complement(3434..3856)
                     /locus_tag="JNY73_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
     gene            complement(3856..4449)
                     /locus_tag="JNY73_001399"
     CDS             complement(3856..4449)
                     /locus_tag="JNY73_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY8047459.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4596..5003
                     /locus_tag="JNY73_001400"
     CDS             4596..5003
                     /locus_tag="JNY73_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY8047460.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5123..6115)
                     /gene="rpoS"
                     /locus_tag="JNY73_001401"
     CDS             complement(5123..6115)
                     /gene="rpoS"
                     /locus_tag="JNY73_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY8047461.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6178..7317)
                     /gene="nlpD"
                     /locus_tag="JNY73_001402"
     CDS             complement(6178..7317)
                     /gene="nlpD"
                     /locus_tag="JNY73_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY8047462.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGAQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7457..8083)
                     /gene="pcm"
                     /locus_tag="JNY73_001403"
     CDS             complement(7457..8083)
                     /gene="pcm"
                     /locus_tag="JNY73_001403"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY8047463.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8077..8838)
                     /gene="surE"
                     /locus_tag="JNY73_001404"
     CDS             complement(8077..8838)
                     /gene="surE"
                     /locus_tag="JNY73_001404"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY8047464.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8819..9868)
                     /gene="truD"
                     /locus_tag="JNY73_001405"
     CDS             complement(8819..9868)
                     /gene="truD"
                     /locus_tag="JNY73_001405"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY8047465.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARNALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9865..10344)
                     /gene="ispF"
                     /locus_tag="JNY73_001406"
     CDS             complement(9865..10344)
                     /gene="ispF"
                     /locus_tag="JNY73_001406"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY8047466.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10344..11054)
                     /gene="ispD"
                     /locus_tag="JNY73_001407"
     CDS             complement(10344..11054)
                     /gene="ispD"
                     /locus_tag="JNY73_001407"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY8047467.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11073..11384)
                     /gene="ftsB"
                     /locus_tag="JNY73_001408"
     CDS             complement(11073..11384)
                     /gene="ftsB"
                     /locus_tag="JNY73_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY8047468.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11578..11901)
                     /locus_tag="JNY73_001409"
     CDS             complement(11578..11901)
                     /locus_tag="JNY73_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY8047469.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11951..12556)
                     /gene="cysC"
                     /locus_tag="JNY73_001410"
     CDS             complement(11951..12556)
                     /gene="cysC"
                     /locus_tag="JNY73_001410"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY8047470.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12556..13983)
                     /gene="cysN"
                     /locus_tag="JNY73_001411"
     CDS             complement(12556..13983)
                     /gene="cysN"
                     /locus_tag="JNY73_001411"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY8047471.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13985..14893)
                     /gene="cysD"
                     /locus_tag="JNY73_001412"
     CDS             complement(13985..14893)
                     /gene="cysD"
                     /locus_tag="JNY73_001412"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY8047472.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15145..16182
                     /gene="iap"
                     /locus_tag="JNY73_001413"
     CDS             15145..16182
                     /gene="iap"
                     /locus_tag="JNY73_001413"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY8047473.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16268..17419)
                     /locus_tag="JNY73_001414"
     CDS             complement(16268..17419)
                     /locus_tag="JNY73_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY8047474.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(17613..17765)
                     /locus_tag="JNY73_001415"
     CDS             complement(17613..17765)
                     /locus_tag="JNY73_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8047475.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18029..18763)
                     /gene="cysH"
                     /locus_tag="JNY73_001416"
     CDS             complement(18029..18763)
                     /gene="cysH"
                     /locus_tag="JNY73_001416"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY8047476.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(18837..20549)
                     /gene="cysI"
                     /locus_tag="JNY73_001417"
     CDS             complement(18837..20549)
                     /gene="cysI"
                     /locus_tag="JNY73_001417"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY8047477.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20549..22348)
                     /gene="cysJ"
                     /locus_tag="JNY73_001418"
     CDS             complement(20549..22348)
                     /gene="cysJ"
                     /locus_tag="JNY73_001418"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY8047478.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22664..23029
                     /gene="queD"
                     /locus_tag="JNY73_001419"
     CDS             22664..23029
                     /gene="queD"
                     /locus_tag="JNY73_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY8047479.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            23107..24378
                     /locus_tag="JNY73_001420"
     CDS             23107..24378
                     /locus_tag="JNY73_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8047480.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPSWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24369..24629
                     /locus_tag="JNY73_001421"
     CDS             24369..24629
                     /locus_tag="JNY73_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY8047481.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24646..25221
                     /locus_tag="JNY73_001422"
     CDS             24646..25221
                     /locus_tag="JNY73_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY8047482.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25369..26262)
                     /locus_tag="JNY73_001423"
     CDS             complement(25369..26262)
                     /locus_tag="JNY73_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY8047483.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADID
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(26226..27005)
                     /locus_tag="JNY73_001424"
     CDS             complement(26226..27005)
                     /locus_tag="JNY73_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY8047484.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26983..28392)
                     /locus_tag="JNY73_001425"
     CDS             complement(26983..28392)
                     /locus_tag="JNY73_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8047485.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28414..29868)
                     /locus_tag="JNY73_001426"
                     /pseudo
     CDS             complement(28414..29868)
                     /locus_tag="JNY73_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29938..30724)
                     /locus_tag="JNY73_001427"
                     /pseudo
     CDS             complement(29938..30724)
                     /locus_tag="JNY73_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31042..32319
                     /locus_tag="JNY73_001428"
     CDS             31042..32319
                     /locus_tag="JNY73_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8047486.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     HGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32346..33824
                     /locus_tag="JNY73_001429"
     CDS             32346..33824
                     /locus_tag="JNY73_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8047487.1"
                     /translation="MSKKYIIGIDGGSQSTKVVIYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHILFDVQMPGTVLGHITPQGALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         8282 a         9075 c         8423 g         8501 t
ORIGIN      
        1 tcatcaatat cagtcatagt atttcctcgg ttatttcaaa tcagctttgt ttcctttatt
       61 taacagtccg ttcccgcgga ctgttttttt attcaggcca gcggatcatc ttcttcctgc
      121 atggcaaaaa gtttttgcat ccccagccgg aaatcgcgtt caaattcttc gtcatgctct
      181 tcttcatatt ccggttgctg aactgccacc acattaccaa ccgggcgata aacattactc
      241 agccagggtt cagcctgttt gtgttccaga atctgaatac cttcatcttc agcatcacga
      301 attttttcct cagcgcgttt gcgcattcct ttaatacgct gctgttgttt gtgatattca
      361 gcactgacag caaaggctgc gcgtttattg ccgttccata tcgcctcaca gataaagctg
      421 ccgtccttgc gacgtaccgt aattcgctcg gcgtcatgaa tgtcataact gataagcact
      481 ttgcgaccgt gttcgtcacg caactcccgc gcgtagtaaa tattgttcag ccactgaatt
      541 tcgcagcgtc ttaccggtcg ttccaccatt gggcggaaca tgtcgcgcag ctcaagatca
      601 ctcagccact caatttcggt gtcttcttct gccaggcgtt tttttctgaa ctctgtcggg
      661 ctgtaatgcc gtccgttcgg tttcatcggc agctcagaat gcgggcggtt gttgtaccat
      721 tccacgcctt cacgaatagc ctcaataagc tccgcccacg atggcagctc gcgcattgcc
      781 gctttctggc ggtcgttcag aggcttgtcc tgttgcaggg cgttaaacgc tgaacgcaga
      841 tcacggttaa gtacacgcaa cgattcccgg tcagcaccct tgccaaaata ggtgcgatac
      901 ctcctggcta tacgcatcgg cagtgtccgg ttcaggcgtt caataatgcc gcgtccctgt
      961 ggattaccgg caataccggt agggtgttta atccccaggc gtggcagaat acccacgacc
     1021 tctttgtcca gcatgtcagc cgtttcaccg gaaccgttat ccgagtaata caggaacggt
     1081 tttccgtggt tgcgaatgcc gtgctgtatc gcaccggcaa cggcgaacac gttttcggca
     1141 aggtcaagac tccagccaac aacaaaccgt gttccaccat cgatcacaaa cgtgacttcc
     1201 ggtgagaatg gacgcccgtg gatcggatgc gcacatttca gcttcatacc gtgaccgtca
     1261 ccaatccaga cataattcac cggcatagct gtccagtcgc ggcgggtgaa tccttcaagc
     1321 tgacggtatt cactgccggt aacgcgccct ttttgtttca ccacttccgg cagttttttc
     1381 atggcatacc ggacaacatc ataggaaggc atcacctcca gcatgtacgg ttcgtccgca
     1441 tggcgacgct gccattctgc gacaaaatcc tcgtaggctt ctgacatggg tctgccgttc
     1501 acctggcgat actgcgccag aaattcaggc agccagctga tttcttccgg ttttatttcg
     1561 tcgcgttttc ccggtgccag taacagcagc cgttcggcgg cgttctgcga ctggttgtat
     1621 ttcagaaccc actggtaaag tggatcgcgg ctgatagtac gattagcgcc acgttttgca
     1681 ttggcgttaa ctgcggcctg ttgcaggcgc tccggcagct cacctttacg ggctttttca
     1741 acaagaaagt taatggcttt cagtcgcccg aggttaccgg cgctttccag cttcagcact
     1801 tccaccacca gcgcagctct tgcttcagat acccagcgct ggttttcggt cagcgcattc
     1861 aggcgttctt ccatcagttg tggagaaccg cgatatgctt cgattgcctg gacagcgtca
     1921 ggattgcgtg tttttctggt aacagctttc acttcagaaa cattgttctc tgtagccacc
     1981 agctgttcca catagcgttc acgcagtgcc tttcgtgtta tttcaggcag acaatcaatg
     2041 tggtactcaa tggcttttgt gccttcccga cggcgactaa cgtcaccaag atcacgagaa
     2101 aaacgctgta acgcctgtcg gatgcctttt gtggtggctg gcattcctgg tacgccgagc
     2161 aggtcattta cggtaacgta cgactgcata tcacgacgcc ttcctgataa atccatcgtt
     2221 acgataccgg ctgggccaga tgatttctgg cggtacacct atagcgtcag aaattacgcg
     2281 ttcgtatttt ttgtttggtg tgcgaactac cgctttcagt gaatcctgag cataaccagc
     2341 atggagtgaa agctcccgga aggaagacca cccgttatct ttgagcgcca gctgaatctt
     2401 cagcgaaggc caatcgcggt cactatcttc tctttgtctc atctttcctt taacctacaa
     2461 aataacccgc gcggatatcc gtatggataa agattaagat accaaagatt ccctataaag
     2521 ggttctttgg tgcttttatg gtttattttt taatttcatt atttatcaat gaattagata
     2581 gaaaggaacc aagaatgaga aaagcacccc aaggttcttt tcctgccgat ggaaaagaac
     2641 cgattatcga aagaatcttt aggttagtag aacgataccc ttcaagaaat gcggctgctc
     2701 gggcatgggg gatcaatgaa ggcactttaa aaaattatta caacaggcgg gattacgcac
     2761 cgataccacg cccgcatcag ttaaaaaaga tagcagaaag cgaaaatgtt accttggaat
     2821 ggctcatgac gggagagggt gatgttgaac taaaagattc caaagaaccc aaaaaagaga
     2881 atgcggataa tgttgatgct caaattctaa tgttcttgtc atttcttaaa ccacaagaaa
     2941 aacaacaagt agctgatgtt cttgggagaa aaggagcgga gcaattactc attcttctgg
     3001 atgagcacat ccaagagttg cacgctctag ttggagttcg tagagccata gcgttaagcc
     3061 ttggtaattt gccagacgaa aaggttagag agatttacgc gttgtccgaa gctaagggcg
     3121 atcacttaaa tctacctcag aaagcagcca gcgcataacc cagtgcaagt gcctagctgt
     3181 gctaaaatta cattggtttt ggcgcttgca cccatcagcg ctataataaa cacctgaatc
     3241 gtttttgaac ggtctttaaa ggctattgta agaacctgtg agttatcttg aatgttttat
     3301 tgatatcgaa ccgtttaaca tttttggact cgctttatct tgaaaacctt gagttcctaa
     3361 cttgttgatt ccaatctatt cccgtctttt ttaatctctt cccggttatc ttgaataaat
     3421 tacctagtca tacgagatag aaaccgccat cgccatcatt caggcgaaac agcggcagaa
     3481 tatcgaacag gttgatctct tcgccatcga cggtgttctg cgcccaggct ggattggcgc
     3541 ggcgctccgg ggcaatcggg aagttatccc agcggcggat aaactcatca atctgctttt
     3601 tgaccggggt gtttggtggc aggccaaggg aaatcgcgtg gttctgccag gaaccgatgg
     3661 tattcatcgc cacgcgggca tcggtaaagc cgcgaatatt atcaaaccac agcgcgggcg
     3721 cgccgtcgcc gatacgcccg gtggcgttag ctgctgctgc cagatccggc tcagcgttca
     3781 cttcttcgct gattttcagt aactggccgt ggtcatcaag cgcctgtaaa aagctgcgta
     3841 aatcatcaaa tgccattatt cattctcctg agaaaaattc cgggcctgcg gcaatccttg
     3901 ccagcgcctg gcgtggggat gttcgaggcc aaattgatcc agcacgcggg ctaccacatg
     3961 gtggacaatg tcatctaccg tttcgggatg gttataaaag gcaggcatcg gcggcaccat
     4021 cgccacgccc atgcgtgaaa gtgcgagcat attttcgaga tggatggtgc taagcggcat
     4081 ttcacgcggc accagcacca gtttgcggcc ttctttgagc acgacgtccg ccgcgcgccc
     4141 taccaggcca tcggcgtaac cagcgcggat accggcgagc gttttcatac tgcacggaat
     4201 aacgatcatg ccgtcagtac gaaaagaacc ggatgagatg atcgccgcct gatccgccgg
     4261 gttatggctg aagtctgcga gggcagcaac atcgcgagcg ctgtaaggcg tttccagttc
     4321 aatgatggtt ttcgcccact tcgacatcac cagatgagtc tcgacattcg gcatctcacg
     4381 cagcgcttgc agtaatgcca caccaagagg cgcaccggta gcccctgtca tcccgacgat
     4441 cagtttcatt gcttattctc ttaattattc gcatacgaac gttattatta acatactcct
     4501 tcatttccac agcggcaagg attgaaaagt aacaacatct tcttcgtcac taaaaaaccg
     4561 ctatgatagc gacagagaat tagcccggag tttacatggc gttacgaaat aaagcgtttc
     4621 atcagttacg acagcttttt cagcagcaca cggctcgctg gcagcacgag ttacctgacc
     4681 tgaccaaacc acagtacgcg gtgatgcgcg ccattgctga taaacctggt attgaacagg
     4741 tggcactgat agaagcagca gtcagcacca aagcaacgct agcagaaatg ttggcaagaa
     4801 tggaaaatcg tggcttagtc agacgtgaac atgacgtcgc tgacaagcgg cgacgctttg
     4861 tctggctgac tgccgaagga gaaaagctac ttgctgcggc gatacctatt ggtgacagcg
     4921 tggatgagga atttttgggg cgtttgagtg gagaagagca ggagctgttt atgcagttgg
     4981 tgcgcaagat gatgagcaca tagaatgctg attgccgggt aggacgctga cgtgtcttat
     5041 ccaggcgaca aaaacacaag cttcccaaac aaaagaaaaa ggccagcctc gcttgagact
     5101 ggcctttctg acagatgctt acttactcgc ggaacagcgc ttcgatattc agcccctgcg
     5161 tttgcaggat ttcgcgcaaa cggcgcaggc cttcaacctg aatctggcga acacgttcac
     5221 gggtgaggcc aatttcacga cctacatctt ccagtgttgc cgcttcgtac cccagcaaac
     5281 cgaatcgacg tgccagtact tcacgctgtt tggcgttcag ctcgaacagc catttgacga
     5341 tgctctgctt catatcgtca tcttgcgtgg tatcttccgg accgttctct ttttcatcgg
     5401 ccaggatgtc cagcaacgct ttttcggaat caccacccag cggggtgtct accgaggtaa
     5461 tgcgctcgtt aagacgaagc atacggctga cgtcatcaac tggcttatcc agttgctctg
     5521 cgatctcttc cgcacttggt tcatggtcca gcttatggga caactcacgt gcggttcgca
     5581 ggtaaacgtt cagctccttt acgatgtgaa tcggcaaacg aatagtacgg gtttggttca
     5641 taatcgcccg ttcaatcgtc tggcgaatcc accaggttgc gtatgttgag aagcggaaac
     5701 cacgttccgg gtcaaacttc tctaccgcgc ggatcagccc caagttgccc tcttcgataa
     5761 ggtccagcaa cgccagacca cgattgccat aacggcgggc aatttttacc accagacgca
     5821 agttactctc gatcatccgg cggcgagagg cgacatctcc acgcagtgcg cgacgcgcaa
     5881 aataaacttc ttcttcggcc gttaacagtg gtgaataacc aatctcacca aggtaaagct
     5941 gagtcgcgtc caacacacgc tgtgtggctc cctgcgataa cagttcctct tcggccaaat
     6001 cgttatcact gggttcctct tctactaagg ccttttcgtc aaaaacctca actccgttct
     6061 catcaaattc cgcatcttca tttaaatcat gaactttcag cgtattctga ctcataaggt
     6121 ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa agcctggttc cgccgattta
     6181 tcgctgcggc aaataacgca gcgggtttac ggatttcccc ttgtaacgaa tttcaaaatg
     6241 caagcgtgtt gaactggttc cggtgctacc catggttgct attttttgcc ccgccttcac
     6301 ttcttgttgt tcccggacca gcattgtgtc gttatgggcg taggcactca ggtaatcatc
     6361 attatgtttg atgataatca gattaccgta gccgcgcagc gcgttaccag cataaacaac
     6421 gcggccatct gcggtcgcga taattgcctg tcctttgctg cctgcgatat caatcccctt
     6481 gttgcccccc tcagaagcgc caaaggtttc gatcactttg ccctcagtcg gccagcgcca
     6541 ggtggagata ggcgtactgg ttgatgtact gctgacagtc ggctcggttg tgcttgctgt
     6601 tggtaccgtt acaggcgctg tgaccgtggt cgcagttggc ttgttgttcg gcaacatttt
     6661 gttagcactc tgtgcacccg aagactcaga atacgtaatt gtcggttgcg acgcaacagc
     6721 aacggtggaa ttttgtgcag gcttgatcac aactccttgc tctgctgcgt cggcctgggt
     6781 aatagcattt ccgccagtga ttggcgtacc ggaagcatta cccacctgca aggtctgacc
     6841 aacgttcagc gcgtatggtg cctgaatatt gttgcgctga gcaaggtcac ggaaatcgtt
     6901 gccagtaatc caggcgatat agaaaagtgt gtcacctttt ttcacggtat aggtactgcc
     6961 gctataactg cctttcggaa tgttcccata ctgacggtta tagacgatgc gtccgttttc
     7021 catctgtacc ggctgctgag ctactggctg cactggctgg atttgcggtt gttgagtagc
     7081 ctgaatttgt ggctgctgta ccggctgaat ttgcggttgc tgcgctgtag acgtcgtccc
     7141 tattttcggc ggcggcgtaa tcaacatacc agaattagta tttgcaggcg cattgccatt
     7201 aacggagctg accggtgccg gtggatttga agtgtcagaa cagcctgcca gccatagcga
     7261 aaccagtgac aaagccgcaa tgcggcgaac ggtgaatttt gggcttcccg cgctcattta
     7321 tcccccagga aaaattggtt aataaccagt gacataatta ccgtgcaagg caccctactg
     7381 aacactggaa aagatgttca cgatacgctg acctacggca aaataaccag gaaaaatcca
     7441 ggtatttcct cacgttttaa gccagctcac ccttcactaa agggacaaag cgcacggcct
     7501 ccacggtatc gataataaat tcgcctcccc gacgacgcac ccgtttcaaa tactggtgct
     7561 cctcccctac gggtaagacg agaatcccgc cttcgtccag ctgcgtcatt agcgcagttg
     7621 gaatttccgg cggtgccgcc gtaacaatga tagcgtcaaa cggcgcacgt gcctgccaac
     7681 cttgccatcc atcaccatga cgggttgaaa cattatgtaa atcaagattt ttcaggcggc
     7741 gacgtgcctg ccactgcaag cctttaatcc gttcaaccga gcaaacatgc tggacaagat
     7801 gcgccaggat tgccgtttga tatcccgaac cagtgccaat ttccagcacc cgcgactgcg
     7861 gcgtcagctc gagtaattcg gtcattcgcg ccaccatata cggctgcgaa attgtctgcc
     7921 cctgacctat cggcaaggcg atattgtccc aggctttttg ttcaaacgct tcatcaacga
     7981 atttttcacg cggcacggcg gcaagtgcat tcagcacctg ctcatcctga ataccttgcg
     8041 cacgtaattg atccagaagt gcttgtacgc gtctgcttac cattgcgtgc caactcccac
     8101 gctgtttaac cagtctgaaa ccacatcttg cgcgctatgc gcagttaaat ccacatgcag
     8161 cggcgtgatg gagacatagc cctcatctac cgcagcaaaa tcggtccccg gaccggcatc
     8221 acatttaccg cccggcgggc caatccagta cagcgtattg ccgcgcggat cttgctgcgg
     8281 gatcacctga tctgccggat gtcgtgtacc gcagcgcgtc acgcgaatac ctttgatttg
     8341 atccaagggt aaatccggaa cgttaatatt aagaatacgt ccggtgcgca gcggctcttt
     8401 acacagtgcg cgcaaaattg aacaggttac cgccgcggca gtgtcgtaat gtttatgccc
     8461 gtcaagcgag acggccagcg ccggaaaacc taaatgacgg ccttccatcg cggcggctac
     8521 cgtaccggaa taaataacat catcccccag attcggcccg gcgttaattc cggacacaac
     8581 aatgtccggg cgcggacgca tcagagcatt cacgccaaga tagacacaat cggtcggagt
     8641 tcccatttgc acagcaatat caccattttc aaaggtaaac gtgcgcaggg aggattccag
     8701 tgtcagagaa tttgaagcgc tgctgcggtt acgatcgggg gcgaccacct gaacgtcagc
     8761 aaactcacgc aaggctttcg ccagcgtttg tataccgggt gcatgtaccc cgtcatcatt
     8821 actcagcaat atgcgcataa tcacctgttg tgttgataag ttcccttaca acgctggttg
     8881 caaaactacc cgccggaagc cagaaacgga tctctacggt gacgtcatcc caccagttcc
     8941 agcttaattg ttgcggatac agcagcatcg ctctgcgcgc ggcttcaact ttttcgcgca
     9001 ccagtaaagc ttgtaattca gtttctgcgg cgacagctgc ttgttcgaat gccagcgctt
     9061 cacgctgagt tccccattcg ccactgcccg gcaatgcggc ggttatcatc agctctttat
     9121 cgttgacgcg acgctgtaat tccgccagtt cttcggtggt tgctacaaac cagctaccac
     9181 gtccggctaa ttgtagcgca tcgccgtcaa caacttgatt aacgtctgct tttttgaggc
     9241 gctcagcaac aatctgatta aacaacgcat tgcgggctgc cgacaaccaa aaactccgtt
     9301 tattgcgatc gcgcaccgga gtattggttt gcgcccagcg cagcgcgccc tgcaagttgc
     9361 taccgccaat cccaaaacgt tgggcaccga agtagttcgg tacacctttt acgcaaatat
     9421 cgatcagacg ttgttcaacg tcatcgcgat tgctcacttc gcgcagaacc agggtaaagg
     9481 cgttaccttt cagcgcccct aaacgcagct tgcgcttgtg ccgcgcatac tccagcacct
     9541 ggcagccttc cagttgaaag gcgctcagat cgggcatttc cttgcccggc acgcgagcac
     9601 ataaccactg ttccgttaca gcatgtttgt ctttttgccc agcgaagctg acttcacggg
     9661 aatgaatttt caggaatttc gccagtgcat ccgccacaaa acgggtattg cagccgtttt
     9721 tgaggattct aaccagaata tgctcacctt caccatcagg ctcaaagccc aaatcttcca
     9781 ccaccacaaa gtcttccgga ttggctttca gcagcccggt gccttgcggt ttaccgtgga
     9841 ggtaagtgag attatcaaac tcaatcattt tgttgcctta atgagtagcg ccaccgcttc
     9901 acaggcaatc ccttccccac gtccggtaaa tccaagtttt tccgtagtag tggctttcac
     9961 gttaacatca tccatatggc agccgagatc ttcggcaata aacacgcgca tttgtggaat
    10021 gtgcggcaac atcttcggtg cctgagcgat gatagtgaca tcgacgttgc caagggtata
    10081 acccttcgcc tgaatacgac gccaggcttc gcgtagcagc tcgcggctgt cggcaccttt
    10141 aaatgccgga tcggtatccg ggaacagttt gccgatatcc cccagcgccg ccgcgccaag
    10201 caatgcatcg gtcaacgcat gaagcgccac gtcgccatca gaatgcgcca gcaatccttt
    10261 ttcgtaagga atgcgtacgc caccaatgat aattgggcct tcaccgccaa aggcgtgtac
    10321 gtcaaaaccg tgtccaattc gcattatgta ttctcctgat ggatggttcg ggtgaggtaa
    10381 aactcggcca gtgccaaatc ttccgggcgc gtgactttaa tgttatccgc gcggccttcg
    10441 accaactgcg gatgaaatcc gcaatattcc agcgccgagg cttcgtcggt aatggtagcg
    10501 ccttcattta gagcgcgcgt cagacagtca tgtaacagct cacgagggaa aaattgcggc
    10561 gtcagcgcgt gccataagcc gttgcgatca acggtatgag caatggcatt tttgcccggt
    10621 tcggcacgtt tcatagtatc gcgcactggt gcggcgagga tccctcccgt gcggctggtt
    10681 tcgctcaacg ccaacaatcg cgcgaggtca tcctgatgca aacaaggacg agcggcgtca
    10741 tgcaccaata cccactgcac atcgccagcg gctttcagac ctgccagcac ggaatcggca
    10801 cgctcatcac cgccatctac aacggtgatt tgcggatgat tcgccagagg aagttgtgca
    10861 aaacggctat cgccaggact tatggcaatg acgacacgtg tcacccgggg atgcgccagc
    10921 agcgcatgca ccgagtgttc aagaatggtt tgattaccga ttgagagata ttgcttagga
    10981 cattccgttt gcattcgacg gccaaatccg gccgccggaa ccacggcgca aacatccaaa
    11041 tgagtggttg ccatgttaat tcccgggctg atttatcgat tgttttgccc cgcagactgt
    11101 gcgcgcttcg acgcgtcagg caccagacga taaaaagttt cgcccggcct ggtcatgctg
    11161 agttcattac gcgcacgctc ttcgagcgcc tcctggccgc cattgagatc gtcaatttcg
    11221 gcaaaaagtt gatcgtttcg cgctttaagt ttcgcgtttg tagcttgcag tgccgccaca
    11281 tcatcattga cgcgggtata gtcatgtata ccgttcttac cgaaccacag cgaatactgt
    11341 agccagacca gaatagccag caacagcagc gttagtttac ccatcctgcc ccctgaaaaa
    11401 ctgcatcatc atcccatgca tccgaagacg actctacatc ctctgttggg gataccgcga
    11461 caacgcgggc aaatgtacca catttgtcca ttgttacgta tacccagggc gtgcagaaca
    11521 taatctcatt attagttacg gtttgaatta tgaacagagg agacaagaaa gtacaaatta
    11581 gcccagtagc cacataaaca gtgcgccaaa cataatgcct actgtcacca gggtgaaaac
    11641 aatactgtag cgtagctttc cgtccatcaa tgaatgcagc gcaatcccca ccactaccgc
    11701 gacaggcatc agtgccagaa agaaaggcca ggtgtagata aagaagaaca gcgtgttaga
    11761 gccataaatc aacatcggca tcgccagcgc aaataaccag gagataaaac cgaccacggc
    11821 accaggcagt gaccatgtag tttcttcatc ctcagtaagg ctgtcgttat ttgttagtgt
    11881 aatgttatgg ctattacgca tatttgatcc tgttactttg acgaaccggg catggaaacc
    11941 cggtggtgtc tcaggatctg ataatatcgt tctgtctcaa cagatctaat aattgctgta
    12001 ccaaatttgt tactaattgt tcaccattga gatgaatttc tgccgattca ggctcttcgt
    12061 aaacggaatc tattcccgta aagttgcgca gttcaccggc acgcgctttc ttatataagc
    12121 cttttggatc gcgggcttcg caaatcgcca gcggcgtatc gacaaacact tcaataaagc
    12181 gcccttctcc tacgcgttcg cgaaccatct ggcgttcggc gcggtgtggc gagataaatg
    12241 cggtcagcac caccagtccg gctgcaacca tcaaattcgc cacttcaccg acgcgacgga
    12301 tattctcttt acgatcggca tcgctaaaac cgagatcgct gcataatccg tggcgaacat
    12361 tgtcgccatc cagcagatac gtactgacgc cgagtttatg taacgcctcc tccagcgccc
    12421 cggcgaccgt tgatttaccg gacccggaga ggccggtaaa ccacagcact acaccacgat
    12481 gaccgtggtg tagttcgcgt tgttgcacag tgaccggatg actatgccag acgacgtttt
    12541 cgtcatgcag cgccattatt tatcccccag caaatcgcgc gcgccccagt gcggaaagtg
    12601 gcgacgaacc agggcattca attccagttc gaatgcactg aattcagatg acgcagctgt
    12661 tgcctggcta actggctcgt gcaccatacc ggcacccacg gtcacattgc tcaggcgatc
    12721 gataaaaatc agcccaccgg taaccggatt ttgctgatag cgatctaaca ccagtggctc
    12781 gtcaaaagtg agatccacca ggccgatgcc gttcagcggc aggttttcaa cttcgcgttg
    12841 ggtaaggtta ttaatatcaa cctgataacg aatgccatca acacgagcac gcgtcttctt
    12901 accggcaatt ttgatgtcat aactctggcc cggggaaagc ggctgttccg ccatccatac
    12961 cacatccacc gacgcactct gcaccgccgg taacgcttcg tctgccgcca gcagcagatc
    13021 accacggctg atatcgatct catccgtcag caccagggtg atcgcttctc cggcaaagac
    13081 ttcttcgcga tcaccatcaa aagtcacgat ccgcgcgacg tttgattcca caccagaggg
    13141 cagcactttt acacgttgcc cgacttccac gcgaccggat gccagcattc cggcgtagcc
    13201 acgaaaatcg agatttgggc ggttaacgta ctgcaccggg aagcgcattg gctgggcatc
    13261 caccactcgc tggatctcca cggtttccag cacttcgagc agtgtcagac cgctgtacca
    13321 cggcatactt tcactttgcg aagccacgtt gtcaccttcc agtgcggaga gcggcacaaa
    13381 gcggatatcc agattacccg gcagctgccc ggcaaaggtc agataatctt cacgaatacg
    13441 ggtgaacgtc tcttcactgt aatccaccag atccattttg ttgatcgcca cgaccagatg
    13501 tttgatcccc aacagtgtgg agataaaact gtgacgacgg gtttgatcga gcacgccttt
    13561 acgggcatca atcagtaaga tcgccagttc acatgtcgat gcgccagtcg ccatattgcg
    13621 agtgtactgc tcgtgccctg gggtgtcggc gataataaat ttacgcttct cggtagagaa
    13681 atagcggtag gccacatcaa tagtgatgcc ctgttcgcgc tcagcttgca ggccgtccac
    13741 cagcagagcc agatccagct tttcgccctg ggtgccgtga cgcttactgt cgttatgcag
    13801 cgatgagagc tgatcttcgt agatttggcg ggtatcgtgc agcagacgac caatcagggt
    13861 acttttgccg tcatcgacgc taccacaggt cagaaaacgc agcaggcttt tatgttgttg
    13921 cgcaatcatc caggcttcga cgccgccttc attggcgatt tgttgtgcaa gtacggtgtt
    13981 catcttaaaa atacccctga cgttttttca gctccatgga tcccgcctgg tcgcggtcaa
    14041 tcacgcgacc ctgacgttca ctggtggtgg aaaccagcat ctcttcgatg atttccggca
    14101 gtgtttgtgc atttgactcc accgcaccgg tcagcggcca gcagcccagc gtacggaaac
    14161 gcaccatccg ttttttgatc acttcgcccg gttgcaggtc gatacggttg tcatcaatca
    14221 tcatcaacat accgtcgcgt tccagaaccg gacgttccgc agcgagatac agcggcacaa
    14281 tgtcgatatt ttccagccag atgtattgcc agatatcctg ctcggtccag ttagagagcg
    14341 ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt gtagttgtgc cacagctccg
    14401 ggcgctgatt tttcggatcc cagcgatgga agcggtcacg gaaagagtag attcgctctt
    14461 tagcgcggga tttctcttcg tcacggcgcg caccaccgaa ggcggcatca aaaccgtatt
    14521 tgttcagcgc ctgtttcagg ccttcagttt tcataatatc ggtatgtttc gcgctgccgt
    14581 gcacgaatgg attaatcccc atcgccacgc cttccgggtt tttatgcacc agcagctcgc
    14641 agccgtaggc tttcgccgta cgatcgcgga actcatacat ctcgcggaat ttccagccgg
    14701 tatcgacatg cagcaacggg aaaggcagcg tacctggata aaacgccttg cgcgccagat
    14761 gcagcatgac gctggaatct ttaccgatag agtagagcat caccggattt gagaattctg
    14821 ccgccacctc gcgaataatg tggatgcttt ccgcctccag ttgccgcagg tgagtaagtc
    14881 gtttttgatc cataaccgtt cctttgcaat accgctattt tcttgccatc agatgtttcg
    14941 actataggga gcgtaagaga acgaatgaaa ttaccaatta gaatgagtag ttccttaacg
    15001 gaataacgat ttggcaaagc taatatcaaa aagtgcttaa ggcaccggat ttcgggcgtt
    15061 taggaagatt tgaaattgtt ttagcgcagc ggcagtttca tactatggcg gtaaaaaaat
    15121 ttgcatggta tttaaggact cactatgttt tccgcattgc gccaccgtac cgctgccctg
    15181 gcgctcggcg tatgctttat tctccccgta cacgcctcgt cacctaaacc tggcgatttt
    15241 gctaataccc aggcacgaca tattgctact ttctttccgg gacgcatgac cggaactcca
    15301 gcagaaatgt tatctgccga ttatattcgc caacagtttc agcaaatggg ttatcgcagt
    15361 gatattcgga catttaatag tcggtatatt tataccgccc gcgataatcg caagaactgg
    15421 cataacgtga caggaagtac ggtgattgcc gctcatgaag gcaaagcgcc gcagcagatc
    15481 atcattatgg cgcatctgga tacttacgcc ccgctgagcg atgctgacgc agatgccaat
    15541 ctcggcggac tgacgttaca aggaatagat gataacgccg caggtttagg tgtcatgctg
    15601 gaactggcag aacgcctgaa aaatacgcct accgagtatg gtattcgatt tgtggcgacc
    15661 agcggcgaag aggaaggaaa attaggcgct gagaatttac tcaagcggat gagtgacacc
    15721 gaaaagaaaa atacgctgct ggtgattaat ctcgataact taattgttgg cgataaattg
    15781 tatttcaaca gcggtgtaaa aacccctgaa gcagtaagga aattaacgcg cgacagggcg
    15841 ctggcaattg cacgcagtca cggaatagcc gcaacgacca atccgggttt gaataaaaat
    15901 tatccgaaag gcactgggtg ttgtaatgac gcagaaatat tcgacaaagc gggcattgct
    15961 gtactttcgg tggaagcgac taactggaat cttggaaata aggatggtta tcagcaacgc
    16021 gcaaaaacac ctgccttccc ggcgggaaat agctggcatg acgtaagact ggataatcaa
    16081 caacatattg ataaggcact tccaggaaga atagaacgtc gctgccgtga cgttatgcgg
    16141 ataatgctac cgttggtgaa ggagctggcg aaggcgtctt gatgggtttg aaaatgggag
    16201 ctgggagttc taccgcaggg gcggggaatt ctaagtgata cccatcatcg catccagtgc
    16261 gtccggttta cttccgggaa aaccgcaccg tattgcgccg gtttatctcc gctggagcgg
    16321 ggaacacctc tggcgaatag aaagcaagcc aggttttgac cagtcgatcg tacatggcat
    16381 tggccgttgc cattgttggc aggccaaaaa acagcccctg agcgttaccc gcagccatca
    16441 acctgtgcgc caggataagc gccgcttctg ttttacctgc gccggtcaca tcttccagaa
    16501 taaacaactg tggtcctggc tgattgatat ccagatccag caccttttgc tgtaacggtg
    16561 tcgtattctc aataaaagga aacagcgtat taattccggt gaaaggcgcg gtttctgctt
    16621 gtggcggaaa gacggttaat gcgttttgag cctgaactaa agccttttgc cagtaatctt
    16681 caatatccat tgcctgtgcg acgcgtggaa aaaatcgcgt tgacgaccct gtccagtctg
    16741 caagtacgac ggttgcagag atataccagg aaagttgttt tataagttca acgccctcgt
    16801 catcatccca gaatgtgggg atctctattc gcggaaacag tggcttgatt tcaaggagaa
    16861 aatctcgtgc tgcagctttg tcttcaggca gaaaattatc caattcatcc atacgatcag
    16921 ggggccgacc atgatgccct gtcgtcacgg gcatccacat ttctatcact cgcctaagtt
    16981 tgcgcggaga gagcgaagat gaaggaagta actcctgaca ttcacttaaa taatgattcc
    17041 acaaccagta gcccagcgtt gaatgagaga ttttttcgta attttttctg gcaccttcct
    17101 gaatcttgaa ttcagggggc aggtaaagtt gttgaaaaga gcgggcaaat tttccaatat
    17161 cgtgccagca cagcaaccag gcgaaaaatt gagctgcctg ttccttatca gaaatcccta
    17221 attgacgaaa ataatcagcc agcccgaagc aatttctttt aaccattaaa tagcccattg
    17281 cggccacatc cagcaaatgc cagcaaagaa ggtgatagtc gtcgctaccc tctttctcgc
    17341 cacgtcggtt ttttccccag aaatcaaaga aagtcacaat atttttatcc ttcagtaaac
    17401 ttaaaggata tttacgcata ttttaaaaac tatctgtgat atatatcagc caataaaaca
    17461 tttacattat gaataaatta atgattttca tttgaaattc ataataataa atagagacta
    17521 tatattcaca taaaaaatat aaattttaaa aaagggtgca ggagaccaaa ccatcacaat
    17581 tcagtacgcg gggttttacc cccgcgtggt ggctacttct tcgattcgta agcgagaact
    17641 gccttaaact ccataccacg ttctctgata ctcaactcac ataacgagtc acctaccatc
    17701 aacgtgaatc ccagtactgt acaacacagt acgatgattg ccacaagggc atattttgtc
    17761 agcatgtctt gcctccctgc aaagaagaga ctaacatccg aattgctacg ttcgaacgcg
    17821 agcctcaggt tgatagaaat atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc
    17881 ttaacgccag acagcctcaa gcacccaacg ccattctata cctgttaatt ttccctgcga
    17941 tgttcacctt taaatgccgg aatgctgctc gcaaaataaa aacgccccca tcatttctga
    18001 cagaggcgtt taatttatcc ggcaatattt acccttcgtg taatccacat tcccttttta
    18061 agccaaagaa acgcgtttct tcttccgcca tgccgggttc ccatttacgg gttgtatggg
    18121 tatcgcccac cgataaatat ccttcgtccc ataatgggtg atatttcagg ccatgttttt
    18181 gcaggtactg ataaatagtt cggttatccc agtcgataat cggcagcact ttaaatacgc
    18241 cacgctgaat tgccagcacc ggtaaattgg cacggctgcc ggattgttca cgccgcaggc
    18301 cagcaaacca cgtttgcgca ttcagttctt tcagcgcccg gttcatcggt tcgactttgt
    18361 tgatgtcatt gtacttttca atgccttcaa cgccctgttc ccacagtttg ccgtagcgtg
    18421 cttcctgcca ggcggcgctt tcggtagcgc ggtacacttt caggttgagc ttgagtttgt
    18481 ccgttaactc gtcaataaag cggtaggttt ccgggaacag atagccggtg tcggtgagga
    18541 tcaccggaat atccgggtga atctgattca ccagatgcag gctcaccgcc gcctgaatgc
    18601 caaagctgga agaaagtaca tattcaccgg gcagattatc cagcgcccag gcaacgcggc
    18661 cttcagcgtc cagtttttcc agttcggcgt tagtttctgc cagcgccaga atgcgatcta
    18721 ccttcggcag ttcgttcagg gcgtttagat cgagtttgga catagattcc tcactgtttg
    18781 ccttgcctga tgcgacgctc acgcgtctta tcaggcctac aagaccgggc tgatggttaa
    18841 tcccacaaat cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag
    18901 tcgccgaagc cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc
    18961 gacgccagga tttccggctc ggtgatgttt tctttataca tccgtgggat acgtgtccca
    19021 atgcggttgc cgccaagatg caggttgtag cgacccggtg ctttacccac caggcccact
    19081 tccgccagca tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc
    19141 tcatcgctga caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg
    19201 aaacgctccg cttccgccat cgccagcggg caagtcggga atgacacgca ggccatcgag
    19261 ttttcacgct gcggcgtgac ggcattcatt aacccgctct ctttggcaat tttctcgatc
    19321 ttcgctttct cgctttccgg cacaccggcg atgatcagat tctggttcgc cgtaatgcgg
    19381 aaatcacctt tgtggatctt cgcgatctcc agcaggccag ttttcagcgg acgccccgga
    19441 taatcaagga tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgccc
    19501 ttaacccagc caatacgatc gccgcgtcct gtaaattcat acgggcggat cggttcaaat
    19561 ttgatccccg cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagt
    19621 gtgtattttg ttttggcatt tttacgatcg gttcggttac cccagtcacg ctgagttgtc
    19681 acgacggctt ccgccaccgc tagcgtatgc tctagcggca gatagccaaa ctcactcgcc
    19741 gtgcgggcgt aggttttctt gttaccgtgt tcgatagaaa gcccaccgcc caccagcagg
    19801 ttgaagccca ccagcttgcc gttttccgcg atcgccacga agttcatgtc gttggcgtgc
    19861 agatcaatat cgttctgcgg cgggatcact accgtggttt tgaacttacg cggcaggtag
    19921 gtctgaccaa ggatcggttc ttcatcggtg gtggcgactt tttcctgatc gagccagatc
    19981 tccgcatacg cgcgggtacg cggcagcaga tgttcagaaa tcttcttcgc ccactcgtac
    20041 gcttctgcgt gcagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg
    20101 tcgttagcag tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg
    20161 acgttctttt tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg
    20221 ccatagatgg tgttttcacc ggcaaattta tcgatcgcct gccactgttt ggtggtgata
    20281 actccacccg gcagacggca gcgcaacagc atcgcgtggc gcggctccag cttctgttca
    20341 gcacgttcgg cgcggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc
    20401 aggaagttat cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg
    20461 ccgcgcaggt agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc
    20521 actaaaggcc ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta
    20581 cgcgcagctc acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa
    20641 tcacttccag aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat
    20701 aaatgtgggc accgtcattg atccagcgcc acagctccgc gccctgttcg cgcagtttat
    20761 cttgtacgta aactttttct ttttggtcgc gcgaccaggc gagatcgatc cgcgtcagca
    20821 caccctcttt gacgtagcgc tgccactcca cctgatacag gaagtcttcc gtaaaatgcg
    20881 gattgccaaa gaacagccag tttttacctg gcgcttcgtc agcggcgcgt tgctgcataa
    20941 aggcgcggaa cggcgcaata ccggtgcctg ggccaatcat aatcaccggg gtttccaggt
    21001 tagcgggcag gcggaagtta tcgttatgtt cgataaatac gcggacttcg ccctcttctt
    21061 ccacgcggtc agcgaggaag ctggaggcac caccggcacg ggcgcggcct tccacgtcgt
    21121 aacgcaccac accaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcaa
    21181 tggaatagag acgcggggtc agcgggcgca gcagattaat tagcgcttcg gcatcaagct
    21241 gtgccgggga gaaacgcacc atgtcaacaa tcagcgtcgt cgcggcgtaa tgctgtaact
    21301 tcgctttatc gcccaccagc ggcagcagtg tttcactgcg ggtaagcgtg gcgtaattct
    21361 caacaatgtt ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag
    21421 gcaacgtttt gccctcgaca gtgacaggtt catcgcctgt cagccacagc agttcgacaa
    21481 gttctttcac cagtgccgga tcgttctgat accagacgcc cagcgcgtca cccggctggt
    21541 aacgcaggcc cgagtcgcct aagtcaattt cgatatggcg aacgtctttt tcagagttac
    21601 gcccagtaat tttctggtta acagagaggc tagccaccag cggcgcgtct ttgctgtacg
    21661 ggctggtgtg gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag
    21721 gcgcacgcga tttaagcgca tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt
    21781 attcaacatc agaatcgaca cggtcgagca ggcgttcacc acccagttcc gccagcttgc
    21841 tgtcaaaatc tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca
    21901 cggcaaacgc ggtgttttcc agctttggcg cttttttgga gaacaggaac ttatgcagcg
    21961 cgacggcttc ttccggcggt tccccttccc cttgcgttga cgtcactacg atgagcagtt
    22021 tttcgctggc gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt
    22081 ttgctgctaa taaatcatcg cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg
    22141 aagcagagat aatcgttata cccggcattt ctgcggcggg cgctggcgtc actgcaagcg
    22201 caacaggctg ctggttgagc acgccccaga aatagccaga aacccaggca agctgggtag
    22261 gcgttaaatc ggtcgtggcc gcctgaaggc gtgccagttg ctccgggttc aacggaagca
    22321 acgcggaagg tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt
    22381 ctgcgccctg tcagcgccca taaaacagaa gagaaggtaa ggttaacggg gtgaacggtg
    22441 tggattaaag acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata
    22501 gtctttaaca acaaaataga ttaaccaacc taatgaaaaa caaatgaatt tagccaatca
    22561 ttaagataaa tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc
    22621 tttccggtac tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt
    22681 atttaaagat ttcaccttcg aagccgctca ccgcttacca catgtcccgg aagggcataa
    22741 atgtggtcgc ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga
    22801 tccgcatacg ggctggatta tcgatttcgc tgaactgaaa gcggcgttta agccaactta
    22861 tgagcgcctc gatcaccatt atctcaatga tattccaggt ctggaaaacc caaccagcga
    22921 agttttagca aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt
    22981 gatggtaaaa gaaacctgca ccgcagggtg tatctatcgc ggcgaatgat aagagtgtgt
    23041 cggcggtcaa tttcccttta gtaacgctat gttagggtgt tgtgttctgg atatctgggg
    23101 catgacatgg aagacgactg cgacattatt attattggtg ccggtattgc aggcaccgct
    23161 tgcgcgttac gctgcgcgcg agcggattta tccgttttgt tactcgaacg cgctgaaatc
    23221 cccggcagca aaaatctttc cggcaggcgg ttatataccc atgcactcgc ggaactcctc
    23281 ccgcaatttc atctgaccgc gcctcttgaa cgacgcatca ctcacgaaag cctttccctg
    23341 ttaacgccgg atggcgcaac gacgttttcc agcttacagc ccggcggtga atcctggagt
    23401 gtattacgtg cacggttcga tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa
    23461 tgcatccccg gagcgacggt ggatgcactg tatgaagaaa acggcagagt ctgtggcgtt
    23521 atttgtggtg acgatattct ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc
    23581 gtcctggctg aacgtcacgg gttagtgact cgtcctgctg gcgaagcgat ggcgttgggg
    23641 atcaaagaag tgctgtcgct ggaaacatcc gctattgaag aacgttttca tctggagaat
    23701 aacgaaggcg cagcgttgct gttcagcggc ggaatctgtg atgacttacc cggcggcgca
    23761 tttctttata ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt
    23821 acgcaaagtc gtgttccggc aagcgagctg ctgactcgct ttaaagcgca tccggcagtg
    23881 cgcacgctta tcaaaaacac ggaatcactg gagtatggtg cgcatctggt gccagaaggt
    23941 ggcttgcaca gtatgccggt gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg
    24001 ttgcgcagtt gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctgactggc
    24061 gcacaggcgg cggcacaaac gctaataagc gcctgccagc accgcgagcc gcaaaatctg
    24121 tttgcgcttt atcatcacaa cgtagagcgc agcctgctat gggatgttct acaacgttat
    24181 cagcatgttc cggcgctttt gcaacgccct agctggtatc gggcgtggcc tgcgttaatg
    24241 caggatattt cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag
    24301 ttattctggc gtcatttacg tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg
    24361 agtctgcgat gtctgtagcc cgtaatctct ggcgcgttgc tgatgcgccg cacattgttc
    24421 cggctgactc cgttgagcgt cagatggtac aacggttgat taacgcctgt ccggtaggtc
    24481 ttttttctct cacacaggaa ggtggcttac gtattgacta tcgcggctgc ctggagtgtg
    24541 gcacctgccg tttgctgtgc gacgaatcca cactacaaca gtggcgctat ccgccttccg
    24601 gattcggcat cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac
    24661 ctgctccgcc agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggcc
    24721 atcgattctg aatgccagtt tatttccgtg ttgtacggca atatctgcac catcagtaat
    24781 atcgttaaaa agattaagaa cgccgggaaa tatgctttta ttcatgttga tttgctggaa
    24841 ggcgcgtcaa ataaagaagt ggtgattcag tttttgaaac tggtgaccga ggcggatgga
    24901 attatcagca ccaaagcatc gatgctaaaa gcggcaagag cggaagtttt tttctgtatt
    24961 catcgcctgt ttattgttga ttcgatttcg tttcacaaca ttgataagca agttgcgcaa
    25021 tcgaatccgg attgtattga gatcctgcca ggctgcatgc ccaaagtgct gggctgggtg
    25081 acggaaaaaa tccgccaacc gctgattgct ggcgggctgg tatgcgatga agaagatgcg
    25141 cgtaatgcga ttaacgcggg tgtcgtggcg ctttccacca cgaataccgg agtctggacg
    25201 ttagcgaaaa aattgctttg acgggataat cgtaaccaat tgaatttgtt ttgatttgtt
    25261 ggccgcacgc cacatccgac attcagcgcc tgatgcgacg cttaacgcgt cttatcaggc
    25321 ctacaggtcc ccagtccaga accgtaggca ggatcatccg gcagcacgtt attggtattc
    25381 tgcgtgaatg tttgtcacca gtgcctccag caccactttc cagtcatcaa ccacgtcaat
    25441 atctgcctgt gaaaataccg ctgcgctggc gtcatgatta atcgccacca taaacttgct
    25501 attgcgtacg cccgccatta acgctgctgc gccggaagcg ccaacaacaa tgcacacctc
    25561 aggtgccagc agatgtccgg aaataccaat cactttttca gcatcaacgc caccgttcat
    25621 cactcgcgcc cgactgtagc ccacttcggt tcccagcttt tctgccagca taacgatttc
    25681 ctggttatct gcctctccgc cctgccccac caccagcacc cgtctggcct cagctaatgg
    25741 gtcgcggcta acatttttca gatcctctgt gctaaccagc cagtcgggaa gcgcacctgg
    25801 gacaatgttc agttgctgca ttccagaggg caacgtggcg tttttcgctg ctcccgcctg
    25861 acgcgccagg gaaaggcata acgggcgttt ttcagtttgc agcgtcgccg ttaacgcatt
    25921 tccccagtgg gatttgcgca tgcttacggt tggaatatct aacgaagtca cctgacagat
    25981 gcttgcacca tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa acgttcccgg
    26041 cggaaagaga acaacgtccg caggcgttcg ctgccactgt tccaccatcg catccagcac
    26101 ctgttccgcc actacgggct gaggttctat ttgccagtgc gccagcgtac aaccgctgaa
    26161 atcctgcgcc gccagccagc tcgctattgc ggcattctcc tgatttattg tcacaattgc
    26221 gatattcatg gctgcatcct ttggcgtaaa tagtcctgcc atagcttttg agctttttct
    26281 gctaccgttt gcccatcaat cagcgtcgct ccgcatcgtt gctctgctct tgccagttgc
    26341 agacactgca tcgctggcat ttcggcagca acagttttac ggataatttc tgctttcccc
    26401 gcagccattc gctggcgcat tcccggtact ggcaaggcca cttcaccgca ctgacgcacg
    26461 gcaatcaccg caggcaagcg aacccggcaa cagcgcaacc catgttcagt acgctgttcg
    26521 agggtgataa acagcgcgtc gagagtgaaa cgctccacct gggtgaagca gggccagccc
    26581 agcatttccg ccagcaaaaa tggcgtttgc ccattctgcc cttcgctgct ttggcagccg
    26641 gtaattatca gatccagcgg attctgacgc tgccattcgg caatatggcg agcgacaaat
    26701 tccggcgcaa agcgcagatc tgccgccgtc tccagcaata cagcttcctc aaacccaaga
    26761 gccatgagat agcgcagcca gtgcaacgcc cgttcatccc ccatgctcaa cgcggttaaa
    26821 gacatcggcg taccgttttt tctctgcgct aacagcagcg cggcagcggc ctgttcatca
    26881 gcaccgagta aacttcgcag tagcgaaaca tccggtccgc tattaccctg agccgccgcc
    26941 tgccactctt tttccgccag cattccggca tccggttcgg ctttaaacgc taacagaatg
    27001 ttcattcgca cctcctacat ttcccgccgc caccagcggg agggctttag tttctggtgc
    27061 ccatagccag gtcacaacaa aaccaaccaa caacactgtc gccaggagca ataaggtgac
    27121 ttgcattccc cactgcgcca gcacccacgg cagcaggcca gtacttaccg ccgcgccaag
    27181 tcgactcatg gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag gaaaactttc
    27241 cgcaggcaaa atgcccacca gattactgac tgccgaaatg gtggtgctga agagaacaaa
    27301 aagcagcagc gttaatgaac tgccggaagg caaacaggcc attactacca gcgttgccgc
    27361 cagcagcaaa aaacttccca gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa
    27421 tcccagcagc gcgcccacaa ttaacaacgc attaagcatc aggctggcag tcagcgcatc
    27481 ttccagacca atagtctggg cgatagttgg cagccaggta taaatcacaa accatgggat
    27541 tacgaggcag acaaagaaga cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa
    27601 caaggttttg atgtgtttat gggtcgccgt taccacttca tcgcccagta aaacatgggg
    27661 accaaaatag cgatgcacga tagcgtgagc ttctgcaaaa cgcccctggc acagtagcca
    27721 acgtggcgat tctggcgttc cccagcgtaa taacgtaatc aacaacgcgg gcaaagctgc
    27781 cgatgccagc aaccagcgcc aggcctccgg gttttcggaa ataaagtgat gtccggcaat
    27841 acttgccaac acatagccta cggtccacac cacgctgaat gcgcccagca aaataccgcg
    27901 atggcggcgc ggggaaaatt cagccagcaa ggtgtgacct actgagtaat cgcctcccag
    27961 accaatgccg atcaaaatgc gcagcccaat aagatgctct ggcgtggtgg caaaaaattg
    28021 caagaacgaa gcgagcgtaa tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat
    28081 atggtcggag atccacccaa gaaccaggct gccaagaaac aaaccaagga gcgccgagcc
    28141 gccgatcatt cccgccataa acggcgtcag ttgcatggcg ggcgtaagtt gaataatggc
    28201 gtaaccaatg acgccgagaa cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc
    28261 aattcgacag tgaaaacggt taagcggtaa atcatccatt cgcaccggtg aagtgttcat
    28321 ctttacccct tcattgtccg gtaataagaa cggcttctcg cctgagaagc cgaaaaggca
    28381 acgccccgtc atcgcggaac gctgcctgat acattatttt tcaatcggat agatagtacc
    28441 cgtattcata atgccgttag gatcgaactg ctttttcagc ccttccagca acgcccacgc
    28501 gctgccgtgt tccagcttgc tccagtgaac gcgatgttta ccaatgccat ggtggtgcac
    28561 catcgaaccg ccgaggcgaa tggtttcttc gcagatgatt ttgttgagcg gattgtagta
    28621 cttgtcgatt tcctcttccg gcttacagtc aacaacgttg tagtcataga cgaagtacat
    28681 gttggtgccg ttctgatagc tatgagagga gtgaccgccc agcatggtga tgtcgtcggc
    28741 gtgcgggaac tcagtacgaa tacggttaat aacgctttcg tagatttcgt ggatgcagct
    28801 ccagcagccg gacacttcgg tggtaaagcc catgttgccg gttttgagga tctgcacacg
    28861 ttcggcagcc actttatccg gtccccagtt caggttgtta aaccaggttt caatcagttt
    28921 gctgtccacg cgctggcact gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt
    28981 cgccttcgca atgcgagggt taccttcagc cataaagatc agcacccatt ttccgtcggc
    29041 aaaatgggtg aagtgttggg tgccatcttc agcgtcatac aaacgagcga tcgacggacg
    29101 atacccttcc accatgattt cacgcaggat gttgaagccg gttttcatgt cttccaggat
    29161 atagccgtag aagaggttgt tttccggggt gtatttaaag attttcactg ttacttcagt
    29221 gatatagcac aatgcacctt cgttgccgat gatgatgtga cgaatgtccg ggccagccgc
    29281 gcggcgtggc acgtttttaa tgcgtgtgac ggtgccatct gccaacactg cctccagacc
    29341 aacgaccata tcttcgattg cgccgtagag tgtggagaac tgcccgatac tgcgggttgc
    29401 taccaggccg cccatctgcg ccagcggctt tgactgcgga gaatgccccg tggtgtaacc
    29461 tttttcacgc aacgcgtttt ccagcacttc cagcggaaca ccacattgca ccgtcgcctg
    29521 catattctca atatcaatat taatgatttg attcatggcg gagccgtcga gcaccaccga
    29581 gttttctaca acagtttcca gcccaccttc ggtggcggaa gcaccggtac gcggcacacc
    29641 gttaattttg tgcgcattca taaaattcag cacacgggat acttactctg tggaaccgag
    29701 ttttacgacc gctgccggaa tcggcaaagt ataaatgcca tgaatatccg gaaatttacg
    29761 aaaacggtca atactgtttt tctttaatac tgtttcatcg gtaattacgc gatctgcacc
    29821 aacaatttcc tttagctggt cgacaatcgc tgcgcgagat aaagacatag taattccttc
    29881 ctgataataa aaatggaata atcattaata agagaaaaca gaaaccaccg ttatggatta
    29941 gcgcactaaa taaccgccat cgaccactaa taaatgcccg ttgacataat tcgatgcctg
    30001 acttgcgaga aacacggctg cgcccattaa atcctgagta tcgccccaac ggtttgccgg
    30061 aatatgatca agaacgcgct gattggtttc tggattactg cgtgtcgcca gcataatatg
    30121 ctgttgcata atagccaggg gcgataccat ttacctgaat attatattga cctagttcat
    30181 cacaataagc tttggtgaac ccggcaagag catgtttagt ggcagaatat gcaggtgacc
    30241 attgtccacc taagtaagag aacaatgaac agatattaat aattttgccg cttttctgcg
    30301 ggatcataat ttttgccgct tcatagctta actcgaatgc ggcggtcagg ttcacatcaa
    30361 tcatcggatc ccagtcggca cgaccgaagt ccagcacctt attcagctta caaataccgg
    30421 cattgttaac cagaatatcg acagtaccga agcgctcaca gcaggcagcg ataatcttct
    30481 gcggtgcgcc ttctgcggta atatccacct gcatgaagtc cacttcaaca ccctgttttt
    30541 caatcatttc ctttgtttcg ccgttatctt tgacgaagct aggaataaaa atatttgcgc
    30601 cagctttggc caacgccatt gcaaatgcct gacctaaacc gctattcccg ccggtaacaa
    30661 ttgctgtttt acctttcagg gagaaaaaat ccattgagaa cgcattgaga gattcgattg
    30721 acatagttag ctccaatttc cgtaaggcaa aaaaaagaaa ggtaacttcc cccgcgagag
    30781 ggaagttacc tttctcatta tctctggtaa ctggagataa tttttagcat aacggcataa
    30841 ctgctaatgt gatcgtaatc acagtgtgat attcgtattg tcaaatagtt ttaaaaagca
    30901 gaagcgtgat tactctcaca caacacctct gctttccatg ttatttctta ttgcaattac
    30961 tagagacctg agaaaagtaa tccacctgaa agggcggctt acttttctct tttttttgtc
    31021 tattacaaat gggaatacga tatgcaacac aactcatatc gccgttggat aaccctcgcg
    31081 ataattagtt ttagcggcgg cgttagtttt gacctggctt atttacgtta tatttatcaa
    31141 attcccatgg cgaaatttat gggattcagc aataccgaga taggtttaat aatgagtacc
    31201 tttggtattg cggctattat tctttatgcc cccagcggcg ttattgccga taaattttca
    31261 caccgcaaaa tgattacttc cgcgatgatc attaccggat tactgggtct gttaatggca
    31321 acgtatccac cgctgtgggt aatgctctgt attcaggtcg cctttgcgat aacgacgatt
    31381 ttaatgctgt ggtcggtgtc gattaaagcc gcctcgttgc ttggcgatca tagcgagcaa
    31441 gggaaaatta tgggctggat ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg
    31501 tttaccatgt gggtcttttc tcgctttgca ccggatgaca gcgccagcct gaaaacagtc
    31561 attatcatct acagtgtggt ttacatcttg ttggggattc tgtgctggtt ttttgttagc
    31621 gataacaaca acctgcgcag tgccaataac gaagaaaaac agtcattcca gcttagcgac
    31681 atccttgccg ttttgcgtat cagcaccacc tggtattgca gcatggtgat ttttggtgtc
    31741 ttcacaatct acgccattct gagttactcc accaactatc tgaccgaaat gtacggcatg
    31801 tcgctggtgg cagcgagcta catggggatt gtgatcaaca aaatcttccg cgcgctgtgc
    31861 ggcccacttg gaggcattat caccacctac agtaaagtga aatcccctac ccgcgtgatt
    31921 caaatccttt ccatcatcgg tttgctggcg ttgacagccc tgctcgtcac gaactctaac
    31981 ccgcaatcgg tcgcgatggg gattggcctg attttactgc tgggattcac ctgttacgcc
    32041 tcacacgggc tgtactgggc ctgccccggc gaagcgagaa caccgtctta cattatgggc
    32101 accacggtag gtatttgttc ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt
    32161 atcggccact ggcaagacac cctgcccgct gcagaagcct accgcaatat gtggctgatg
    32221 ggcatggcgg cgcttgccat ggtgatagtc tttacctttt tgctgttcca aaaaattcgt
    32281 actgctgata gcgcccccgc aatggctagc agcaagtaag ccaggaaaat tacaggagaa
    32341 aaatcatgtc gaaaaaatac atcataggga ttgatggcgg aagtcagagc acaaaagtgg
    32401 tgatatacga tctggaaggt aatgtggttt gcgaagggaa aggcttattg cagccgatgc
    32461 acacgccaga tgccgatact gcagaacatc ctgacgacga tttatgggca tcattatgtt
    32521 ttgccggtca cgatttgatg agtcagtttg ccgggaataa agaagatatt gtcggtattg
    32581 gtctgggatc catccgttgc tgccgtgcgt tattgaaagc cgatggcacg cccgctgcgc
    32641 cgttgattag ctggcaggat gcacgcgtaa cgcgtcccta cgaacacact aatcctgacg
    32701 tggcatatgt cacctctttt tcgggttatc tgacgcatcg cttaaccggc gagtttaaag
    32761 acaatatcgc caactatttt ggtcagtggc cggtggatta taagagctgg gcatggagcg
    32821 aagatgctgc ggtaatggag aagtttaata tcccccgtca tatactgttt gatgtgcaaa
    32881 tgccaggcac tgttcttggg catatcacac cacaaggcgc gctggcgaca catttcccag
    32941 ctgggctgcc ggttgtttgt accaccagtg ataaaccggt agaagctctg ggggccggat
    33001 tactggatga tgaaacggcg gtgatttcac tcggtactta tatcgcgctg atgatgaacg
    33061 gcaaagcctt gccgaaagat ccggtggcgt actggccgat tatgtcttct attccgcaaa
    33121 cattgctgta tgaaggttac ggtattcgca aaggtatgtg gacggtgagc tggctgcgcg
    33181 acatgttagg cgagtcgtta attcaggatg ccagggcgca ggatctttca ccggaagatt
    33241 tactcaacaa gaaagcctct agtgtgccac ctggctgtaa tgggctgatg acggtgctgg
    33301 attggctgac caatccgtgg gaaccgtaca aacgcgggat tatgatcggc tttgattcca
    33361 gcatggatta cgcatggata tatcgttcga tactggaaag cgtggcgctg acgctgaaga
    33421 acaattacga caatatgtgt aatgaaatga atcactttgc gaagcatgtg atcattactg
    33481 gcggcggttc gaacagcgat ctgtttatgc agatttttgc cgacgtgttc aaccttccgg
    33541 cacgacgtaa cgccattaac ggttgtgcaa gcctgggggc agcgattaat acagcggtag
    33601 gtctggggct atacccggat tacgcaacgg ctgtcgacaa aatggttcgc gtgaaagata
    33661 tctttatgcc tgttgagagc aatgccaaac gctacgacgc gatgaataaa ggtattttca
    33721 aagacctaac caaacatact gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg
    33781 aattggggaa tgcggattcg atccagagct ggtcgaatgc gtaagtgagg aagaccgggc
    33841 gggaaactgc ccggcctgaa tatacctgaa tggttatccc cgctgacgcg gggaactcga
    33901 ataactgacc aacgtctggc tctctaaatc cggtttatcc ccgctggcgc ggggaacact
    33961 ctaaacataa cctattatta attaatgatt ttttaagcca gtcacaatct accaacttta
    34021 caggatcaca caaacaacac atccattatg ttaaagagca cttaatccat tgattaaaaa
    34081 ggtaaatatt taaaataact ctatacaaac taaaatctac caaacttcgc cgcaataatt
    34141 ttcactccag caaaaaatta acgccgcaga atttgtagag aatgataatg attagtccgg
    34201 gatgggcaag atggatgagt tttcaggggc gacaaataat acaaatgagt ggaatttttt
    34261 acaagttcca cccatttata a
//
LOCUS       DADLZM010000031        32327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLZM010000031 DADLZM010000000
VERSION     DADLZM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.031"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNY73_001430"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNY73_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY8047488.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JNY73_001431"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JNY73_001431"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY8047489.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JNY73_001432"
     CDS             complement(3736..4092)
                     /locus_tag="JNY73_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY8047490.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JNY73_001433"
     CDS             complement(4096..4512)
                     /locus_tag="JNY73_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY8047491.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRSVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /locus_tag="JNY73_001434"
     CDS             complement(4623..5336)
                     /locus_tag="JNY73_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY8047492.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5699..6241
                     /locus_tag="JNY73_001435"
     CDS             5699..6241
                     /locus_tag="JNY73_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047493.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLIQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JNY73_001436"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JNY73_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY8047494.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JNY73_001437"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JNY73_001437"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY8047495.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /locus_tag="JNY73_001438"
     CDS             complement(9042..10457)
                     /locus_tag="JNY73_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY8047496.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="JNY73_001439"
     CDS             10540..11523
                     /locus_tag="JNY73_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY8047497.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JNY73_001440"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JNY73_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY8047498.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JNY73_001441"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JNY73_001441"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY8047499.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14172)
                     /locus_tag="JNY73_001442"
     CDS             complement(13465..14172)
                     /locus_tag="JNY73_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY8047500.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14192..14449)
                     /locus_tag="JNY73_001443"
     CDS             complement(14192..14449)
                     /locus_tag="JNY73_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047501.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKCVFN"
     gene            14767..15282
                     /gene="zur"
                     /locus_tag="JNY73_001444"
     CDS             14767..15282
                     /gene="zur"
                     /locus_tag="JNY73_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY8047502.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15324..15533)
                     /locus_tag="JNY73_001445"
     CDS             complement(15324..15533)
                     /locus_tag="JNY73_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY8047503.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15649..17028)
                     /gene="dinF"
                     /locus_tag="JNY73_001446"
     CDS             complement(15649..17028)
                     /gene="dinF"
                     /locus_tag="JNY73_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY8047504.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="JNY73_001447"
     CDS             complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="JNY73_001447"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY8047505.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17765..18133)
                     /locus_tag="JNY73_001448"
     CDS             complement(17765..18133)
                     /locus_tag="JNY73_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY8047506.1"
                     /translation="MVNNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18304..20727
                     /gene="plsB"
                     /locus_tag="JNY73_001449"
     CDS             18304..20727
                     /gene="plsB"
                     /locus_tag="JNY73_001449"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY8047507.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="JNY73_001450"
     CDS             complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="JNY73_001450"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY8047508.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVASALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="JNY73_001451"
     CDS             complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="JNY73_001451"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY8047509.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22487..24067)
                     /locus_tag="JNY73_001452"
                     /pseudo
     CDS             complement(22487..24067)
                     /locus_tag="JNY73_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24296..25216)
                     /gene="malM"
                     /locus_tag="JNY73_001453"
     CDS             complement(24296..25216)
                     /gene="malM"
                     /locus_tag="JNY73_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY8047510.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKIQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="JNY73_001454"
     CDS             complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="JNY73_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY8047511.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26780..27895)
                     /gene="malK"
                     /locus_tag="JNY73_001455"
     CDS             complement(26780..27895)
                     /gene="malK"
                     /locus_tag="JNY73_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY8047512.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28260..29450
                     /gene="malE"
                     /locus_tag="JNY73_001456"
     CDS             28260..29450
                     /gene="malE"
                     /locus_tag="JNY73_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY8047513.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29604..31147
                     /gene="malF"
                     /locus_tag="JNY73_001457"
                     /pseudo
     CDS             29604..31147
                     /gene="malF"
                     /locus_tag="JNY73_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            31162..32052
                     /gene="malG"
                     /locus_tag="JNY73_001458"
     CDS             31162..32052
                     /gene="malG"
                     /locus_tag="JNY73_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY8047514.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8229 a         8289 c         7801 g         8008 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtgaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acgctgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaagttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aataatgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt ttacgtgcat
    14221 gccataaatt atgcgtgatt cgttgaataa ataaatctat ttttgcaata attatatccc
    14281 gatgacgcag cgctgcataa tcaatagatt taccatatat attatttaat ttacctttga
    14341 ttgaacctct attatccaat ttatctttgg ttgcatcttt attttcgaat tgatttgaat
    14401 tgagtgtttt attaaagtat ccagttgcac cgggtattat cttcaacatg attcatctac
    14461 cttttttgtc gtaccgtgtc ctccttataa ttctttttcc atctggtcac tatcaaaaat
    14521 cgctcataat ttctgaacta caggtatgcg tttaaaccca gttaaataaa tagcgagtta
    14581 acttgaagca atttatttaa aaatgaatta ttaattatta tcgtgttgat aatggtacga
    14641 caaggcaaca caacacctta cctacaaaac agaaaatcaa cattacggca acaataaggg
    14701 ttctcggtgt ttttcctgga acatggtaaa gtaaggacat tcttaacccc cactttgagg
    14761 tgcccgatgg aaaagaccac aacgcaggag ttattagcgc aggctgaaaa aatctgcgcg
    14821 cagcgtaatg tgcgcctgac cccacagcgc ctggaagtgt tgcgcctgat gagtctgcag
    14881 gatggcgcta tcagcgctta tgatctgctt gatttactgc gcgaagctga accgcaagcc
    14941 aagccgccaa cggtttatcg cgcgctggat tttctgcttg agcaaggttt tgtgcataag
    15001 gtggaatcca ccaacagtta tgtgctctgt catctgttcg accagcccac gcatacgtca
    15061 gccatgttta tttgcgatcg ctgcggcgcg gtgaaagaag agtgtgcaga aggcgtggaa
    15121 gacattatgc atacgctggc ggcaaaaatg gggtttgccc tgcggcataa tgtgattgaa
    15181 gcacatgggc tctgtgcggc atgcgtggaa gtggaagcgt gtcgtcatcc tgaacagtgc
    15241 cagcatgatc actctgtgca ggtgaaaaag aaaccgcgtt aagagggcgt acatccttgt
    15301 acacgtcggg caggagggat taattaccag cgatattcat tgcgggtttc ccaatccacg
    15361 acctcttttt ctgcctgatc tttctgataa ccataacgtt cctggatttt accgaccagt
    15421 tgatcacgtt taccttcaat gatcgtcata tcatcatcgg tcagtttgcc ccattgctct
    15481 ttcactttac ctttaaactg tttccagtta ccgccggctt catctttatt cataatcaag
    15541 acctcatcgt taggttgtga atgagagtac gttcactttt cttctgaacg tgagattaag
    15601 tgtagtcagc aatttggctt aggataattt attcagaatt tttaaccgtc acgttgcggc
    15661 aaaccaggta ccgttgcgcc agtgacgccg ccagatagcc gccagagaaa gcccgcgcaa
    15721 cgccagaaag acggttaatg ccagccacaa accatgatta cccagccacg gcagcgtaag
    15781 gagcgtcagc gcaaaacctg cggcggccac cgccatactg ttacgcattt cggcggcacg
    15841 cgttgcgcct ataaacatgc cgtccagcag ataacaccag acgccaacca acggcaaaat
    15901 cacctgccag ataagatagc ggtcagccag ctgctgaatc tgggttaacg acgtcagcaa
    15961 cgcaatgatg tgttcccctg ccagcaaata aaccaccgaa aacagtaacg ctacaatccc
    16021 cgactggcgg cacgctgccc gccatacatc cagcaactgg ctaccgtcgc gcgcaccgta
    16081 tgcctgaccg gagtgcgctt caaccgcgta ggcaaaacca tcaagcgcat aggcggtaaa
    16141 ggtgagtagc gtcatcagaa ccgcgttaac agcgataatg tcactcccca gtcgcgcacc
    16201 aagtacggtg atcgcgccga aacagagttg caacaacagc gaacgcagca tgatatcgcg
    16261 gttaagcgcc agcaagcgac ggaagtttcc tcgccaggca gttttcagca tttcgccgga
    16321 aattccgcgt agtttgagga ttttacgcac cattagcaga ccaatcagca atgttgcata
    16381 ttccgcaata accgtcgcca gcgccgcgcc ctgcacgttc atatgcagcc ccatcaccag
    16441 ccagacatcc agcacaatgt tgaggatatt gccgaccact aacaaaatta ctggcgcacg
    16501 ggcatattgc acgccgagta accaaccgag taataccaga ttcgccagcg acgccggagc
    16561 gcttaaccag cggatttcaa gaaagcgccg cgcctgttcc aggaccgctt cactaccgcc
    16621 aacaatatgc agcgccagat cgataatcgg cgtacgcaac agcgcaatta acgccccggc
    16681 ccccaacgcc aacagcaacg gttgcaccag cgcacgggct aatgcctgag gatttttggc
    16741 accataagcc tgcgcagtca gcccggtggt gctcatgcgt aaaaacagca acagcataaa
    16801 gagaaagctg gtcgccgttg caccaaccgc cacgccgccc aaataaaccg gactatcaag
    16861 atgaccaatt accgccgtat cgaccagtcc cagcaacgga acggtgatat tggagaaaat
    16921 catgggtaag gcgagatgcc agagtgcttt atcagatgaa gtgaggaatg ccatgcagac
    16981 aagcctgatg aagagagatg aaaaacaaac cgcgacgcca ggcggcatcg cggtctcaga
    17041 gatatgttac agccagtcgc cgttgcgaat aaccccaacc gccagccctt caatggtgaa
    17101 gctctgctga cgaaggtcaa cgacaattgg tttaaactcg ctattttctg gcaacagttc
    17161 gactttattg ccctgttttt tcaggcgctt aacggtaact tcgtcatcaa tacgtgcgac
    17221 aacgacctga ccgttacgta catcctgagt tttatgcact gccagcaagt cgccatccat
    17281 aatgccgata tctttcatcg acatcccgct gacgcgcagc aggaaatcag cattcggctt
    17341 gaacaaggaa ggatcgacct gataatgacc ttcaatatgc tgttgcgcca gaagcggttc
    17401 accggcagcc acacgaccta ccagcggcaa cccttcttcc tcttcctgca acagacgaat
    17461 cccgcgtgat gcgccggaaa caatttcaat aacgcctttg cgtgccagcg ccttcagatg
    17521 ttcttcagcc gcgtttgggg aacggaaccc caaacgctgc gcgatttccg cacgcgtcgg
    17581 cggcatacct gtctggctga tgtgatcacg gatgagatca aacacctctt gttgcctggc
    17641 cgttaacgct ttcattccgc cccctgggtg tatatacagt tatactgtga gtatatacag
    17701 caaaaggcga ttttggaacc ataaactgca caataaacca gagatttatc gaaatctgga
    17761 agggttatcc aaaatgcgac cataacagga tgcaccaggt aatcacggcg acgataatgg
    17821 caatcagcac cgcggcggat cccatatctt ttgcgcgtcc ggaaagctca tggtattcag
    17881 agccaattcg gtcaaccact gcttcgatgg cgctattgag gatttccaca atcatcacca
    17941 gcatcacgga gctgataagc agcacgcggg taattgcgtc cacatccagc cagcaggcga
    18001 tgaccaccgc caacaatacc gctacgcctt cctgacggaa tgccgcttcg ttgatccatg
    18061 cagcgcgtaa acctttccag gaatagccag cagctttgat aattcgggtg aatccagtgg
    18121 tattattgac cattgaaaga acctttttac attatgagcg tcaatatcag tgtaccgtta
    18181 aatccgatga ttcgggtttg cgtaacgccg cgcgaaacat gagcggatac cacagaattt
    18241 cccatgactt tctgctatcc ttgccgcgca tttgcattat taaccagagg ctttacatcg
    18301 tttatgtccg gctggccacg aatttactac aaattactga atttaccatt aagcatcctg
    18361 gtaaaaagca agtctattcc ggcagatcct gccccggaac tggggctgga tacctctcgt
    18421 ccaattatgt acgttttacc gtacaactcg aaagcagatt tgctgacgtt gcgcgcccag
    18481 tgtctggcac atgatttgcc tgacccgtta gagccgctgg aaatcgacgg cacgctactg
    18541 ccgcgctatg tgttcattca cggcgggccg cgtgtattca cctattacac gccgaaagaa
    18601 gagtctatta agcttttcca cgactatctc gatttgcacc gtagcaaccc aaatctggat
    18661 gtgcagatgg tgccagtatc ggtgatgttt ggtcgcgcgc cggggcgtga aaaaggcgaa
    18721 gtgaacccgc cgctgcgtat gcttaacggc gtacagaaat ttttcgctgt actgtggctc
    18781 ggtcgcgaca gttttgtgcg tttctcgccg tcagtgtcgc tgcgccgtat ggcggatgaa
    18841 cacggcacgg ataaaactat cgctcagaaa ctggcgcgcg tggcgcgtat gcactttgcc
    18901 cgtcaacgtc tggctgccgt aggcccacgt ctgcctgctc gtcaggatct gtttaataag
    18961 ctgctcgcct cccgcgccat tgccaaagcg gtagaagatg aagcgcgcag caaaaaaatc
    19021 tcccatgaaa aagcgcagca gaacgcgatt gcgctgatgg aagagattgc ggcgaatttc
    19081 tcttacgaga tgatccgcct gactgaccgc attctgggct tcacctggaa ccgattttat
    19141 cagggcatca acgtccataa cgccgagcgc gttcgccagc tggcccacga cggtcatgag
    19201 ctggtgtatg tgccttgcca ccgcagtcac atggactacc tgctactttc ttacgtgctg
    19261 taccaccagg ggctggtgcc gccgcatatc gccgccggga tcaacctgaa cttctggccg
    19321 gccgggccga ttttccgccg tctgggggcg ttctttattc gccgtacttt taaaggcaat
    19381 aaactttatt ccaccgtttt ccgtgagtat ctcggcgaac tgttcagccg tggttattcc
    19441 gtcgagtact tcgtggaagg cggtcgttcc cgcacggggc gtttgctgga tccgaaaacc
    19501 ggtacgctgt cgatgaccat tcaggcgatg ctgcgtggcg gcacgcgtcc gattacgctg
    19561 attccgatct atatcggtta tgagcacgtc atggaagtgg gtacttacgc caaagaactg
    19621 cgcggcgcga cgaaagagaa agagagcctg ccgcagatgc tgcgcggttt aagcaagctg
    19681 cgtaatctcg gtcagggtta cgtcaacttc ggtgaaccaa tgccgttgat gacctacctt
    19741 aaccagcacg taccagactg gcgtgaatct atcgatccca tcgaagcggt gcgtccggcc
    19801 tggttaacgc cgacggtcaa taatattgct gccgatctga tggtacgcat taacaacgca
    19861 ggcgcggcaa acgccatgaa cctgtgctgt actgcgctac tggcatcacg tcagcgctca
    19921 ctcacccgcg agcagttaac cgagcaactc aactgctacc tggatctgat gcgcaacgta
    19981 ccttactcca cggactctac cgttccttca gccagcgcca gcgagcttat cgatcacgcg
    20041 ctgcaaatga acaagtttga agtcgagaaa gacactatcg gcgacatcat cattctgcca
    20101 cgcgagcaag cggtgctgat gacctactat cgcaacaaca ttgcgcatat gttggtgctg
    20161 ccttcgctga tggcggcaat tgtcacccag catcgccaca tctcccgcga cgtattgatg
    20221 gagcacgtca atgtgcttta cccaatgctg aaagcggagc tgttcctgcg ctgggatcgc
    20281 gacgagttgc cggacgttat tgatgcgctg gcaaatgaga tgcaacgtca ggggctgatt
    20341 accctgcaag atgatgagtt gcatatcaac ccggcgcatt ctcgcaccct acagctgctg
    20401 gccgcaggcg cgcgcgaaac gctgcaacgt tatgccatca ccttctggtt gttgagtgcc
    20461 aacccgtcga tcaaccgcgg tacgctggag aaagagagcc gcaccgttgc gcaacgtctc
    20521 tccgtgctgc acggcatcaa cgcgccggag ttcttcgaca aggcggtgtt cagttctctg
    20581 gtgctgacgc tgcgtgatga agggtatatc agcgatagcg gcgatgccga accagcagaa
    20641 acgatgaagg tttatcagtt gctggcggag ttgattacat cagacgtgcg tttgacgatt
    20701 gagagtgcga cgcagggcga agggtaatca gagagaattg ccggatgcgg cgaaaacgcc
    20761 ttatccggcc taccatgacc tgcaaattca ataaattgcg attcaccggg taggcctgag
    20821 aagcgcagcg catcaggcaa cccagaagaa agccggatga tcatccggct tttttacatc
    20881 atcagaaatg ccagtaactc attgccagtc ctaaaaacag taccagacca acatagttat
    20941 tattcataaa tgctttaaag caggcttcac gctcgcggtt ggcaatcagt ttttgttgat
    21001 aaacaaacag cgcgctagcc accacaattg accagtaata tccccagcct aagccattta
    21061 actcaccgat gatcgccatc agtgccagta cgccaatctg caaaatacca ataatcaatt
    21121 tatcgtattg gccgaacagg attgccgtgg atttaatgcc aatcttcaca tcatcatcgc
    21181 ggtcaaccat cgcatactgc gtgtcgtaag ccaccgccca gagaatattg gcgaggaaca
    21241 ttaaccagca actcaatggc accgactcac tcacagcggc aaaagccatt ggaatcgacc
    21301 agccaaacgc cgcgcccagc accacttgcg ggagatgggt ataccgcttc ataaacgggt
    21361 acacccacgc caatgccaga gcggcaatcg acaacagaat ggtcatcgta ttcagcgtca
    21421 gaaccagtaa aaacgaaatc agtaccagca ctacaaacag cgcgcgcgcc tctttctctg
    21481 ttaccgcgcc gctgggaagt ggtcggttcg ccgtgcgctt aacatgacca tcaaacttgc
    21541 gatcagcata atcattcacc acgcaaccag cagcgcgcat cagccagaca cccgcgacaa
    21601 acaccgccag gatccagagc tgaggaacgc ccggtgtcgc cacccacaac gcccataatg
    21661 ttggccagag cagcagtaac gcgccaattg gcttatccgt acgcattaag cgatgaaacg
    21721 ccagcagctt attctgcgtc agactccact ccatattttt ttcctcttag tacaacggtg
    21781 acgccggtaa aaacagttct gttagcaaca gcggtttacc gcttaatcgc aggcgggaac
    21841 gtcgccccca cagtccggca tcacggccta tctcgataaa atcccgggtt agtgtcgatg
    21901 atgtgaacag atagcgtcct aacggcgttt tacccaattt ttgtaacgct agttccggcc
    21961 cgcttaatgt tgatacagga acgacggtac gaccggcaag ccacggttca ccatcggcac
    22021 ataacaaaat ttcacgtaac cagtaacgag actctttcgg cagtagcggc agttcttcgg
    22081 ggatttcatt ctgctcgaca aacccctcgc ggatcatcgt cacactcacc gtttttccct
    22141 gctgttcaaa acgttttgtc atggaatcct ccagcaacag ccagtcgaac agttgcggat
    22201 ccagggcagg gatctcttta aaatagcgca gcgcacgcag ttgcgttaac gcggggtgtg
    22261 acatgccgaa ctctccgtta cataacgtaa aggcattgta tcgcagaaaa gtgtttacgg
    22321 gagatgtaaa gcaaattaag tgatctaatg cgcaacaaaa acgcaacaat cgcatgaagt
    22381 ggtaaaacgc tgttgaacct tacgctttgc cgatgcccgt gctggcgagg gcaaccatag
    22441 gggcatcacc gcccatgatg ctaggaacgg tgatgttttc ctttacttaa atacctttat
    22501 tctttatacg agtagctcgc caactcgcag taaaaattgg gaaaagcgca tatttaccat
    22561 gagatttgga gctgaaaagt gtttcctgag taagattaat aggcaacatc ttatgtctgg
    22621 atttatagat acacgtggaa ttccaattgg tatcttttgt aggattcaac atatcatgta
    22681 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
    22741 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
    22801 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
    22861 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
    22921 tatttttatc aaccagccct ttattcatat taaatctgtt aataaaatgg atcatctgtt
    22981 gggtatcgaa attcacctta ctgaatcgat gttgacgcat aaaattatcg aacttgtcat
    23041 cttccccaga atgtatataa ctacgtataa ttggatcttt tatataagga caatggctaa
    23101 atgttttctt catattctca ataaagtttt tattaacaat attgagaatt tttaattgca
    23161 tcaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
    23221 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
    23281 aatcaatagt taattccaat tgttctgtaa ttcaatctta aaaattccct gtcgacattc
    23341 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
    23401 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
    23461 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
    23521 tatcaagaat attttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
    23581 tgtgtaaacg tcgacattta aatatgacat tatgaactgt tgccttagta aaattgcaat
    23641 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
    23701 tagaaataac gtcaccttta aaatgacaat ttgttaaatc aaaatcagac aggtcgatat
    23761 acccctgcgc atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
    23821 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
    23881 gatttttttc gttccgttgt tttatattta aaaatatatc actgatataa atgaagttat
    23941 ctctgtttaa acctgtcacc ccattgaaat tacgtagtaa atcaagatct actatattat
    24001 ttccttgcac cacatcatgt ttcatcaaag agttattgcc gccagaattt gtgatatgac
    24061 ctatcataaa acctctgtaa tacagaaata aaataacctg gaaaagtagc ctgagcgtcc
    24121 agacactacc ggcgaaagga aaagaatctc tgataatgca ttgaaataat ggacattctt
    24181 agtgaagaag aagtgaagta ctataaaaaa tcgctcatat cccaggaagg aagcataaaa
    24241 aaaggtgcgc caggagacgc accagttgtt gcaaaatcag cactgtgggg cgtaattacc
    24301 ccttgccttt tacactgctg ataaaggtgg aacgggcaga tgtcgatccc aggcgttcag
    24361 cttcatcaag cagttttaac gccttatcaa catcaccttt cgcgacagcg tttttgatcg
    24421 cggtattaaa ataactttcc gtgtcgttga gcatcggctc gcttttcttc accggcaccg
    24481 gagcaggtgc agccacagct ggtgccgccg tgttgcctac cgtaaccgga gctggagcgg
    24541 aagaaccaaa tagtggtcct accaacacgc tggagctgga gttcgttttg actttcagtt
    24601 tcagtaagcc atcgctggta tgacgagcaa ccggatcggg aatatccggg atcgagttac
    24661 cgacgccttt ggcataggct ttagccgggt cgagcagttg ggtcgtctgc tggagatctt
    24721 tttccatggt aaagaccaga acataaagtt tttgctgccc caacgccggt gtcaggcgca
    24781 taacgccttc cagccgatct gcactcatca cgcctagttc ctggtaggtg aaataactgc
    24841 tggggaagaa ggctgatggg gtcatgttct gatcaataat cagcacgttc ggcgcaaaaa
    24901 cgctggtttg tttgttcact tcgctggtca gcgtcagggt cagttcgcca atgtttgccg
    24961 ggacgctgta cgcagcaacc ggaccactga tgccgggaac gttcagttgt tggccgccgg
    25021 tcgccagttg ggtggtctgg attttagatt gatcgaccgg tgtccaggtg agttgttgca
    25081 gcgcggcaga tggaatggct ggcgcgtcgc tggtgttttg cggtacgtag ttaacatcgg
    25141 caaggctaat tccaggcgcg cttgccagta accctgctga taaacagagg gcgatgagac
    25201 ttttattcat tttcattgtt ttcacctcaa aatctggagc tcagcggtag ccaggcaata
    25261 gcgcgctaaa cccgataatc agaggggctt tcgccccttt cagataatga caacctgttt
    25321 ttatgccgga tgcggcgtaa acgccttatc cggcccaggt tttgctatta ccaccagatt
    25381 tccatctggg caccgaaggt ccactcgtcg ctgtcgccac gaccgaagct gccgccgttg
    25441 aaatcagcag gaacggcttt gccgtagttc gggttaacct tgctatcgcc gttgtagtcg
    25501 taaccccatt tctcatccca cttggcgtag gttgcgaaga cacgaatagc cgggcgtgac
    25561 cagatgctgt cgccagcctg ccattgttgt gcgagggtaa ttttgtactg attgttcttg
    25621 tcgccggtgc gctgggattc gacgttgtcg tagccgattt ccatcacggt gctcatgatt
    25681 ggcgtccact tgtacatcgg gcgaatacca acggtccacc acttggtgcc gttgtcgtta
    25741 tcccagttga tatcctggta catacccacg tacatcatgt cccagttgtc gcccatggag
    25801 atcgcaccgt ggtcgaggat acgcagcatg tgaccgttgt tgttgatatt gtaggcaaat
    25861 ttttcgttat caaacgcgac gccagaaccc tgcgacagac ctttaccctg cgaggtcatc
    25921 gagtcagtag cgtactgaac aacaaacttg ttaaagccct tcaggacact ctgagtatgt
    25981 tcagcagtga ataaccagcc gtctttcgat gcgccatcaa ccagacgata gttatcacgc
    26041 aggttggcac gaccgtagtc gacacccagt tctaatgtgc cgcccgggtt gatttccatc
    26101 tgcgctaaac gcacatcgaa aacgtcgttc gcggtttcgt tggtatagtc ataaatattg
    26161 ttgctggcaa aagaggaaga accaccagct tcagaggagc gggttgctgc cagagagagt
    26221 ttaccgaagc caacatcgat gttttccaga ccggcaccag gaccagaaat atcccagtag
    26281 tagaagtcga tcatatgaac gtcatgacgt tggtagaagc gcttacctgc ccagatggtg
    26341 gaacctggca gccattcgat caggtttttt ccctgcacgt ttgcttcacg gaaggccgga
    26401 tcggtagctt cccagtcatt ctgttgcgcg acggaatagg ccacgttagt gtcgaaatag
    26461 aagctcttat cgccctcttt ccacacttcc tgacccaatt ttaattcagc ataagtttca
    26521 cattcgttgc caagacggta tttactttga gcaccggtag tctggaaaca ctgttgttca
    26581 ccgccgctac ctgtccagcc aataccggaa cgtgcatagc cgtggaaatc aacagccatt
    26641 gcctgagcag acattacgcc cgctgcgacg gcaaccgcca gaggaagttt gcgcagagta
    26701 atcatcattc tatctcctga gtcattgctt ttcttttttc acatcacctg tgacaggctt
    26761 tgtgtgtttt gtggggtgct taaacacccg gctccttatg cagtcgacga catgcagtgc
    26821 catcctcacg gaacagatgg caacgctctg gcggcaggcc gatagcgaat gtggcacctt
    26881 cttctaccaa caccacgtcg ttctggcggt acaccaggtt ttgacgaatg gaagggatct
    26941 ggatatagat ttgggtttcg ttgccgagtt gctcgacgac ctgaacttca ccctcaagga
    27001 tgacgtcagc gatatcactc ggcagtagat gttccgggcg aatacccagc gacatattgg
    27061 ctccaacctg gacatcacgg ctttcaacag gcagccagac ttgttgacga tttggcatcg
    27121 gcagctccac ctgcacttga tcgattgcgg tggcggtcac ttttaccggc aggaagttca
    27181 tctttggcga accgataaat ccggcgacaa aacggtctgc cggatagtgg tacagttcca
    27241 gcggtttccc aacctgtgcc acgcgaccgg cgtccagcac cacgattttg tcggccagcg
    27301 tcatcgcttc gacctgatcg tgggtgacgt aaatcattgt gcggcccagg cgtttatgca
    27361 gacgggagat ttcgatacgc atttgcacac gcagtgcagc atcgaggttg gagagcggtt
    27421 catcgagcaa aaatacgctt ggctcggcca ccagcgtacg gccaatcgcc acgcgctgac
    27481 gctgaccacc ggagagcgct ttcggtttgc gatccagcaa atgcgccagt tgcagcactt
    27541 ccgccacctg gttgacgcgt tggttaatca cctctttttt tgcgccagcc agtttcaggc
    27601 caaatgacat gttttctgct actgacaggt ggggatagag cgcgtaagac tgaaacacca
    27661 taccaacgcc gcgttctgct ggcggagtgt cattcatccg tttctcaccg atgaacaggt
    27721 cgccgctggt gatcgtctca agcccggcaa tcatgcgcag taaagtcgat ttaccgcagc
    27781 cagacggtcc gacaaacacc acgaattcac cttcatggat atcgagattg atatctttcg
    27841 ataccacgac ctcgccccag gctttcgtta cattttgcag ctgtacgctc gccatgccct
    27901 tctccctttg taacaacctg tcatcgacag caacattcat gatgggctga ctatgcgtca
    27961 tcaggagatg gcttaaatcc tccaccccct ggctttttta tgggggagga ggcgggagga
    28021 tgagaacgcg gcttctgtga actaaaccga ggtcatgtaa ggaatttcgt gatgttgctt
    28081 gcaaaaatcg tggcgatttt atgtgcgcat ccccacatta ccgccaattc tgtaacagag
    28141 atcacacaaa gcaacggtgg ggcgtagggg caaggaggat ggaaagaggt tgccgtataa
    28201 agaaactaga gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta
    28261 tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt
    28321 ccgcctcggc tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
    28381 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
    28441 tcaccgttga gcatccggat aaactggaag agaaattccc tcaggttgcg gcaactggcg
    28501 atggccctga cattatcttc tgggcgcacg accgctttgg tggctacgct caatctggcc
    28561 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacatggg
    28621 atgccgtacg ttacaacggc aagctgattg cttacccaat cgctgttgaa gcgttatcgc
    28681 tgatttataa caaagacctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
    28741 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
    28801 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
    28861 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
    28921 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
    28981 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
    29041 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
    29101 ctaaaccgtt cgttggcgtg ctgagcaccg gtattaacgc cgccagtccg aacaaagagc
    29161 tggcgaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
    29221 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
    29281 cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
    29341 agatgtccgc tttctggtat gccgtacgta ctgcggtgat caacgccgcc agcggtcgtc
    29401 agactgtcga tgaagccctg aaagacgcgc agactcgtat caccaagtaa tgctgtgaaa
    29461 tgccggatgc ggcgtgaacg ccttgtccgg cctacaaaac tgaaacgcat gtaggcctga
    29521 taagacgcat cagtgtcgca tcaggcagtt gttgtcggat aaggcgtgaa agccttatcc
    29581 gtcctggaat gaggaagaac cccatggatg tcattaaaaa gaaacattgg tggcaaagcg
    29641 acgcgctgaa atggtcagtg ctaggtctgc tcggcctgct ggtgggttac cttgttgttt
    29701 taatgtacgc acaaggggaa tacctgttcg ccattaccac gctgatattg agttcagcgg
    29761 ggctgtatat tttcgccaat cgtaaagcct acgcctggcg ctatgtttac ccgggaatgg
    29821 ctggaatggg attattcgtc ctcttccctc tggtctgcac catcgccatt gccttcacca
    29881 actacagcag cactaaccag ctgacttttg aacgtgcgca ggaagtgttg ttagatcgct
    29941 cctggcaagc aggcaaaacc tataactttg gtctttaccc ggcgggcgat gagtggcaac
    30001 tggcgctcag cgacggcgaa accggcaaaa attacctctc cgacgcgttt aaatttggcg
    30061 gcgagcaaaa actgcaactg aaagaaacga ccgcccagcc cgaaggcgaa cgcgcgaatc
    30121 tgcgcgtgat tacccagaat cgtcaggcgc tgagtgacat taccaccatt ctgccggatg
    30181 gcaacaaagt gatgatgagc tccctgcgcc agttttctgg cacgcagccg ctctacacac
    30241 tcgacggtga cggcacgttg acgaataatc agagtggcgt gaaatatcgt ccgaataacc
    30301 aaattggctt ttaccagtcc attaccgccg acggcaactg gggggatgaa aagctaagcc
    30361 ccggttacac cgtgaccacc ggctggaaaa actttacccg cgtctttacc gacgaaggca
    30421 ttcagaaacc gttcctcgcc attttcgtct ggaccgtggt gttctcgctg atcactgtct
    30481 ttttaacggt ggcggtcggc atggttctgg catgtctggt gcagtgggaa gcgttgcgcg
    30541 gcaaagcggt ctatcgcgtc ctgctgattc tgccctacgc ggtgccatcg ttcatttcaa
    30601 tcttgatttt caaaggattg tttaaccaga gcttcggtga aatcaacatg atgttgagcg
    30661 cgctgtttgg cgtgaagccc gcctggttca gcgatccgac caccgctcgc acgatgctga
    30721 ttatcgtcaa tacctagctg ggttatccgt acatgatgat cctctgcatg ggcttgctga
    30781 aagcgattcc ggacgatttg tatgagcctc agcaatggat ggcgcaggtc cgttccagaa
    30841 cttctttaag attacgctgc cgctgctgat taaaccgctg acgccgctga tgatcgccag
    30901 cttcgccttt aactttaaca acttcgtgct gattcagctg ttaaccaacg gcggcccgga
    30961 tcgtcttggc acgaccacgc cagccggtta taccgacctg cttgttaact acacctaccg
    31021 tatcgctttt gaaggcggcg ggggtcagga cttcggtctg gcggcagcaa ttgccacgct
    31081 gatcttcctg ctggtgggtg cgctggcgat agtgaacctg aaagccacgc gaatgaagtt
    31141 tgattaaggg agataacaaa aatggcaatg gtccaaccga aatcgcaaaa agcacgtttg
    31201 tttattactc acctgctact gctacttttt atcgcggcga ttatgttccc gctgctgatg
    31261 gtcgtcgcta tctcgctgcg tcagggaaac ttcgcgaccg gtagcctgat cccggagcaa
    31321 atctcctggg atcactggaa actggcgtta ggttttagcg ttgaacaggc tgatggtcgc
    31381 attacgccac cgccattccc ggtactgctg tggctgtgga actcggtaaa ggtcgctggg
    31441 atttccgcga ttggcattgt ggcgctctcc accacctgcg cctacgcttt cgcccgtatg
    31501 cgctttccag gcaaagcgac gctgctgaaa ggaatgctga ttttccagat gttcccggca
    31561 gtactttcac tggtcgcgtt gtatgcgttg tttgatcgtc tgggtgagta cattccattc
    31621 attggcctga atactcacgg cggcgtaatt ttcgcgtatc tgggtgggat tgcgctgcat
    31681 gtctggacca tcaaaggcta tttcgaaacc atcgacagtt cgctggaaga agcggctgcg
    31741 ctggatggtg cgacaccctg gcaggccttc cgccttgtcc tgttgccgct gtcagtaccg
    31801 attctggcgg tggtattcat cctgtcgttt atcgctgcca ttactgaagt tccggtcgcg
    31861 tcgctgttac tgcgtgacgt aaacagctac accctggccg tggggatgca gcaatacctc
    31921 aacccgcaaa actacctgtg gggtgacttt gccgccgctg ccgtgatgtc tgcattaccg
    31981 atcaccatcg tcttcttgct ggctcaacgc tggctggtca acggcctgac ggcaggtggt
    32041 gtgaaaggtt aaagatgttg ttctgccaat gttatgccgc tgcaccctca acttacgtta
    32101 tcccaacttg tgactattat tcggcgctcc acggagcgcc tttttttctt tcgtctgcaa
    32161 tctgaatcgt tcgccggtta atattcccat catagaactt attattttta cgttatttgt
    32221 tttcccactt acgataattc tctttcgtgc tctgagtcac ggcaatagta ttgttttcat
    32281 caatttttga taattatcac aattaagatc acagaaaaga cattacg
//
LOCUS       DADLZM010000032        31549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLZM010000032 DADLZM010000000
VERSION     DADLZM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.032"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(2..94)
                     /locus_tag="JNY73_001459"
     tRNA            complement(2..94)
                     /locus_tag="JNY73_001459"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JNY73_001460"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JNY73_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY8047515.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JNY73_001461"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JNY73_001461"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY8047516.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JNY73_001462"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JNY73_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY8047517.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JNY73_001463"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JNY73_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY8047518.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JNY73_001464"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JNY73_001464"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY8047519.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JNY73_001465"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JNY73_001465"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY8047520.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8018
                     /gene="srlA"
                     /locus_tag="JNY73_001466"
                     /gene_synonym="gutA"
     CDS             7455..8018
                     /gene="srlA"
                     /locus_tag="JNY73_001466"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY8047521.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8015..8974
                     /gene="srlE"
                     /locus_tag="JNY73_001467"
                     /gene_synonym="gutE"
     CDS             8015..8974
                     /gene="srlE"
                     /locus_tag="JNY73_001467"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY8047522.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8985..9356
                     /gene="srlB"
                     /locus_tag="JNY73_001468"
                     /gene_synonym="gutB"
     CDS             8985..9356
                     /gene="srlB"
                     /locus_tag="JNY73_001468"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY8047523.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9360..10139
                     /gene="srlD"
                     /locus_tag="JNY73_001469"
     CDS             9360..10139
                     /gene="srlD"
                     /locus_tag="JNY73_001469"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY8047524.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10244..10603
                     /gene="gutM"
                     /locus_tag="JNY73_001470"
     CDS             10244..10603
                     /gene="gutM"
                     /locus_tag="JNY73_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY8047525.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDILFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10670..11443
                     /gene="srlR"
                     /locus_tag="JNY73_001471"
                     /gene_synonym="gutR"
     CDS             10670..11443
                     /gene="srlR"
                     /locus_tag="JNY73_001471"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY8047526.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11436..12401
                     /gene="gutQ"
                     /locus_tag="JNY73_001472"
     CDS             11436..12401
                     /gene="gutQ"
                     /locus_tag="JNY73_001472"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY8047527.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12398..13912)
                     /gene="norR"
                     /locus_tag="JNY73_001473"
     CDS             complement(12398..13912)
                     /gene="norR"
                     /locus_tag="JNY73_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY8047528.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRVVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14099..15538
                     /gene="norV"
                     /locus_tag="JNY73_001474"
     CDS             14099..15538
                     /gene="norV"
                     /locus_tag="JNY73_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY8047529.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15535..16668
                     /gene="norW"
                     /locus_tag="JNY73_001475"
     CDS             15535..16668
                     /gene="norW"
                     /locus_tag="JNY73_001475"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY8047530.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="JNY73_001476"
     CDS             complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="JNY73_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY8047531.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="JNY73_001477"
     CDS             complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="JNY73_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY8047532.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19877..20890)
                     /locus_tag="JNY73_001478"
     CDS             complement(19877..20890)
                     /locus_tag="JNY73_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8047533.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            21147..22604
                     /gene="ascF"
                     /locus_tag="JNY73_001479"
     CDS             21147..22604
                     /gene="ascF"
                     /locus_tag="JNY73_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY8047534.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            22613..24037
                     /locus_tag="JNY73_001480"
     CDS             22613..24037
                     /locus_tag="JNY73_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8047535.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFHEKGVTIDKALGDDEILKNTVDFVSFSYYTSRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24152..24571)
                     /locus_tag="JNY73_001481"
     CDS             complement(24152..24571)
                     /locus_tag="JNY73_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8047536.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24568..24879)
                     /locus_tag="JNY73_001482"
     CDS             complement(24568..24879)
                     /locus_tag="JNY73_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY8047537.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25052..25795)
                     /locus_tag="JNY73_001483"
     CDS             complement(25052..25795)
                     /locus_tag="JNY73_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047538.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(25847..26317)
                     /gene="hycI"
                     /locus_tag="JNY73_001484"
     CDS             complement(25847..26317)
                     /gene="hycI"
                     /locus_tag="JNY73_001484"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY8047539.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26310..26720)
                     /locus_tag="JNY73_001485"
     CDS             complement(26310..26720)
                     /locus_tag="JNY73_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY8047540.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26717..27484)
                     /gene="hycG"
                     /locus_tag="JNY73_001486"
     CDS             complement(26717..27484)
                     /gene="hycG"
                     /locus_tag="JNY73_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY8047541.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPSCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27484..28026)
                     /gene="hycF"
                     /locus_tag="JNY73_001487"
     CDS             complement(27484..28026)
                     /gene="hycF"
                     /locus_tag="JNY73_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY8047542.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28036..29721)
                     /locus_tag="JNY73_001488"
     CDS             complement(28036..29721)
                     /locus_tag="JNY73_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY8047543.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIACDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29739..30635)
                     /gene="hycD"
                     /locus_tag="JNY73_001489"
     CDS             complement(29739..30635)
                     /gene="hycD"
                     /locus_tag="JNY73_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY8047544.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            complement(30638..>31549)
                     /locus_tag="JNY73_001490"
     CDS             complement(30638..>31549)
                     /locus_tag="JNY73_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047545.1"
                     /translation="GVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFR
                     TGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAF
                     VARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVAL
                     AICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKG
                     DRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQC
                     EGSALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         7420 a         8733 c         8102 g         7294 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaacga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tacatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaagttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttgcgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggaa tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgctggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctaggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tggcgaactg tttgtttatc ttggcattgc cagcggtctg acaacgctga
     7861 acctgccact tggcccactg gcggtgagtt atctgctggt tggtctggtc accaatttct
     7921 tccgcggctg ggtgaccgat ctgaccaccg ccatttttga gaaaaagatg ggcattcaac
     7981 ttgaacaaaa agttcacctg gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg
     8041 aacgggtggc tggggcggcc cgcttgagct ggaagctacg ccgggcaaaa aaatcgtcta
     8101 tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg gcacaactta ctggctggca
     8161 ggctattgac ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat
     8221 cgactgtggc ggcacattac gctgcggcat ctatccgaaa cgacgtattc ccaccattaa
     8281 tatccactcg acgggcaagt ccggtccgct ggcgcagtac attgtggaag atatttatgt
     8341 ctctggcgta aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc
     8401 cgtgggccgt gactatgaca ccagtaagaa aatcaccgaa caaagcgatg gtttactggc
     8461 gaaggtggga atgggcatgg ggtccgccgt tgccgtgttg tttcagtctg gtcgtgacac
     8521 catcgacact gtattaaaaa ccattctgcc gtttatggca ttcgtctcgg cgctcattgg
     8581 catcattatg gcttctggcc ttggtgactg gattgcccac ggtcttgctc cgctggcgag
     8641 ccatccactg ggtctggtca tgctggcgct catctgctcc ttcccactgc tttcaccttt
     8701 cctcggccca ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg
     8761 tctcggcaat attccgccgc atctggcttt accggcactg tttgccatca acgcgcaggc
     8821 ggcctgcgac ttcatcccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg
     8881 cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc ggcgcgccga ctgtactgat
     8941 cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc
     9001 agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca
     9061 tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg
     9121 gcgagttgaa aggtgcactc catcccggtt tgcaattttc actcgggcag catcgctatc
     9181 cggtgaccgc tgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc
     9241 tgcgcttcga tggtttaaac gaagcggaat ttccgggcac tgtccatgtg gcaggccctg
     9301 tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa
     9361 tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg
     9421 gtctggctgc cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa
     9481 atgtggcaca agaaattaac gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg
     9541 acgccactag cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttggtc
     9601 gcgtggattt gctggtctat agcgccggaa tagcaaaagc agcctttatc agcgacttcc
     9661 agctcggcga ttttgaccgt tcgctacaag tgaatctggt gggttatttc ctgtgtgccc
     9721 gtgaattttc gcgtttgatg atccgcgacg ggattcaggg tcgcattatt cagatcaact
     9781 cgaaatccgg caaagtgggc agcaaacaca actctggcta cagcgcagcg aaatttggtg
     9841 gcgtcgggct gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt
     9901 cactgatgct cggtaacctg ctgaaatcgc cgatgttcca gtcactgttg ccacaatacg
     9961 cgaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc
    10021 tcaaacgcgg ctgcgattat caagatgtgc tgaatatgct gctgttctac gccagtccta
    10081 aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat
    10141 caacagcgga gatccgttaa ggatctccgt gagactatag aatgcctgat gcgctacgct
    10201 catcaggcat acaggacttc cgccactaca ttaaggaaaa gttatggtat ccgcactcat
    10261 caccgtcgcc gttatcgcct ggtgtgcgca actggcctta ggcggctggc aaatttctcg
    10321 ttttaaccgt gccttcgaca cactatgcca gcaagggagg gttggcgtgg gccgttccag
    10381 cgggcacttt aaaccgcggg tcgtggtcgc catcgcgctg gacgatcagc agtgcatcgt
    10441 cgacatcttg tttatgaaag gactgactgt cttcgcccga ccgcaaaaaa ttcccgcaat
    10501 taccggcatg catgcgggtg atttacagcc cgatgtgatc tttccccatg atccactatc
    10561 acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta
    10621 cttgcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca tgaaacctcg
    10681 tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct cggttgaaga
    10741 attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg tcattctgga
    10801 acatgccgga accgtcattc gtacttatgg cggagtggtg ttgaataaag aggaatccga
    10861 tccgcctatc gatcataaaa cgctcatcaa cacccacaag aaagagctga ttgcagaagc
    10921 tgccgttagt tttatccatg atggcgattc gatcattctt gatgctggca gtaccgtttt
    10981 gcagatggtt cccctgctct cgcgctttaa taacatcacg gtgatgacca acagcctgca
    11041 tatcgtcaat gcgctatccg aactggataa cgaacaaact atcctgatgc caggcggaac
    11101 atttcgcaaa aaatcggcct catttcacgg gcaactggca gagaatgcct tcgagcattt
    11161 caccttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac
    11221 gttcaacgag gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt
    11281 gatggcggac tcatcaaagt ttggccgtaa aagccccaac gtagtttgca gtcttgaaag
    11341 cgtcgataag ctaattaccg acgcaggtat cgatccggcg tttcgtcagg cgctggaaga
    11401 gaaagggatc gatgtgatca taaccggaga gagcaatgag tgaagcacta ctgaacgcgg
    11461 gacgtcagac gttaatgctg gagttgcagg aagcaagccg tttaccggaa cgtctgggcg
    11521 atgattttgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg
    11581 gaattggcaa atcgggccac attggtaaga aaatcgccgc aacacttgcc agtaccggca
    11641 caccggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg atgattgaaa
    11701 gccgcgatgt gatgctgttt atctcttact ccggtggcgc gaaggaactg gatctgatta
    11761 ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac
    11821 cgctgggcct ggcggcgaaa gcggtgctgg atatctccgt agaacgcgaa gcctgcccga
    11881 tgcaccttgc gccgacctcc agcaccgtca ataccctgat gatgggtgac gcgctggcga
    11941 tggcggtcat gcaggcgcgc ggttttaatg aagaagattt tgcccgctcc cacccagctg
    12001 gggcactggg cgctcgcttg ctgaataaag tgcatcatct gatgcgtcgt gatgacgcta
    12061 tcccacaggt agcgttaacc gccagcgtga tggatgcgat gctggaactc agccgcaccg
    12121 gtctggggct ggtggcggta tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg
    12181 gcgatttacg tcgctggctg gtaggcggcg gcgcactcac cacgccagtt aatgaagcga
    12241 tgacgacagg cggcaccaca ttacaggcac aaagtcgcgc catcgacgcc aaagaagttc
    12301 tgatgaaacg caaaatcact gccgcaccag tggtagatga aaacggcaaa ctcaccggcg
    12361 caattaacct gcaggatttc tatcaggccg ggattattta atccttcaat cccagacgtt
    12421 tcgccagccg atgcaggttg gcgacgtcag tttccagcat tcgcgcacag gctgcccagt
    12481 tgtgatgatt ttgtgccagt gcctggcgaa tagtttcacg ctggaacgct tctgtcgctt
    12541 cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc aacgtcacct
    12601 caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgttgcgg gtggctctcg
    12661 ccagcactac cacccgatga atagcatgtt ccagttcgcg cacgtttccc ggaaaactgt
    12721 agtgttgcag taaatttcgc gccccggcac ttaataccac gcgggagagc ccctgccgca
    12781 aacgacactg ctcgcagaaa taccccgcca gcagaatgac atcatcgccc cgctcacgca
    12841 gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa caaatcggcg cggaatcgcc
    12901 ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc
    12961 gcaaactacg gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc ctcagcagct
    13021 tggcctgcaa tgccaacgac aactcgccga tctcatcgag aaacagagta ccgttatccg
    13081 ccatttcaaa cttcccgctg cggttactga tagcgccagt aaacgctcct ttcacatgcc
    13141 cgaacaactc actttccgcc acactttccg gcagtgcggc acagttgaga tagaccagcg
    13201 gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc ttaccggttc
    13261 ccgtctcacc gctgatcaga acgttgagat cggacgccgc cacaatctca atctcttttt
    13321 tcagttgcgt catgccaggg gacaagccaa tcatctgcgt ctgtttcacc gcttcaaacg
    13381 gcgtggcatc gcctggcagc atattctggc tttccagttg ttcaatcagc aacgcattgc
    13441 ttaacgctcc cgccgccagc gcggcaatca gccgtaactc ttcgtcgctg aaaacatcga
    13501 actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat caggttttgt ccgacaaaca
    13561 atggcagacc aacgcaggcg tgaaccttca gactctcctg cccaggaatc aaaccgtcat
    13621 agggatcggg caattcgctg tctgcgggaa agcgcaccac atccccggcg cgggcaatcg
    13681 cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacatcc tttgccagac
    13741 cgtcgatggc aagcggaata aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc
    13801 actccagcac ctgacgtagc gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc
    13861 cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc atctttgcct
    13921 cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag tcattttgac
    13981 tactcattaa tgagcataat tttatttata gagtaaaaac aatcagataa gaaactggca
    14041 cgcaatctgc aattagcaag acatcttttt agaacacgct gaataaattg aggttgctat
    14101 gtctattgtg gtgaaaaata acattcattg ggtgggtcaa cgtgactggg aagtgcgtga
    14161 ttttcacggc acggaatata aaacgctgcg cggcagcagc tacaacagct acctcatccg
    14221 cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca
    14281 gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta accatgcaga
    14341 agaggaccac gccggggcgc tgaccgaact gatggcgcaa attcccgata cgccgatcta
    14401 ctgtactgcc aacgctatcg actcgataaa tggtcatcac catcatccgg agtggaattt
    14461 taatgtggtg aaaactggcg acacgctgga tatcggcaac ggcaaacagc tcatttttgt
    14521 cgaaacacca atgctgcact ggccggacag catgatgact tacctgacag gcgacgcggt
    14581 gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt tcaacgatga
    14641 agtggatcag acggagcttt tcgagcagtg ccagcgttac tacgccaata tcctgacgcc
    14701 gttcagccgc ctggtaacgc cgaaaattac cgagatcctg ggctttaact taccagtcga
    14761 tatgatagcg acctcccacg gcgtggtctg gcgcgataac ccgacgcaaa ttgtcgagct
    14821 gtacctgaag tgggccgctg attatcagga agacagaatc accattttct acgacaccat
    14881 gtcgaataac acccgcatga tggctgacgc tatcgcccag ggaattgcgg aaaccgaccc
    14941 acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa
    15001 tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg atgaataacg tgatgatgcc
    15061 gaaaatcgct gggctggtgg aggagatgac cggattacgc ttccgtaaca aacgcgccag
    15121 tgctttcgga tctcacggct ggagcggcgg tgcggtggat cgcctttcca cgcgcctgca
    15181 ggatgcgggt ttcgaaatgt cgctcagcct gaaagccaaa tggcgaccag accaggacgc
    15241 tctggagtta tgccgtgaac acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct
    15301 gccgcagagc acggtgaata cggtagttaa agaagaaacc tctgccacga cgacggctga
    15361 cctcggccca cgaatgcagt gcagcgtctg ccagtggatt tacgatccgg caaaaggcga
    15421 gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gtcccggata acttcctctg
    15481 cccggaatgc tccctcggca aagacgtctt tgacgaactg gcatcggagg caaaatgagc
    15541 aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc
    15601 aaacaggacg ccactattcc attaaccctg attgccgccg acagcataga tgagtacaac
    15661 aaacccgacc tcagccatgt tatcagtcag gggcaacgtg ccgacgacct tacccgccag
    15721 acggcgggtg aatttgccgg gcagtttaat ctgcgcctgt ttccgcacac ctgggtgacg
    15781 gatatcgatg ccgaagccca tgtggtgaaa agccagaata atcagtggca atacgacaag
    15841 ttagtgctgg caaccggtgc cagcgccttt gtcccgcctg tgcctgggcg tgagttaatg
    15901 ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga
    15961 cgcgtgttga ttgtcggcgg tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt
    16021 gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc attaatgcca
    16081 ccagaagtaa gcagccgctt gcagcatcgg ttgacggaga tgggcgttca tctgctgttg
    16141 aaatctcagt tgcaggggct ggaaaaaacg gattctggca ttctggcaac gctggaccac
    16201 cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc ggaaacagcc
    16261 ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa
    16321 accagtaatg ccgatattta cgcgctgggc gattgcgcgg aaattaacgg tcaggtattg
    16381 ccgttcctgc agccgattca acttagcggg atggtgctgg caaaaaatct tctcggcaat
    16441 aacacgccgc tgaaactacc ggcgatgctg gtgaaaatca aaacgccgga attaccgctg
    16501 catctggcag gcgaaaccca gcgtcaggat ttacgctggc agattaatac cgaacgccag
    16561 ggaatggtgg cgcgcggcgt tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag
    16621 gatcggatga aagaggcatt tgtattgttg aaaacgttgc cggtgtaggt gggctactgt
    16681 gcctaaaatg tcggatgcga cgctggcgcg tcttatccga cctacgggga cgcatgtgta
    16741 ggccggataa ggcatttacg ccgcatccgg caagggtgtc caaatgcaac acgctttatc
    16801 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    16861 aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    16921 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    16981 tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat
    17041 catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac tgctgccaga
    17101 aagtggcgag atccagttga ttgtccaccc gcggcatcgt caccggatgc gtcactccgt
    17161 cgcatgaggc tgcgagcgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17221 ctggcgcaca gcccagtgcc gccgccactg catcgaacaa ccgcccacac gatgatgcca
    17281 gcggcgcgtt aattccacgc tcaatggccc gcgccagtac gctccagttt tgctgttgca
    17341 cacttgccgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca
    17401 ggtttcgcca cggctgcttc gccgccaaat cgccacccgg aagcgccact gcaggcaagc
    17461 cgcccaggtg ctcacattcg cgatagttca cccgcaggca ctcgccgccc cacaaagcgc
    17521 cattctcccc cataccgata ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg
    17581 gccactgatg ctctgccaga cacgccgctg catgggcatg atgatgcagt accgtttgcg
    17641 tcggcagatt catttcgctc gcccactggc tggagacata gcccggatgc acatcatgca
    17701 caatgtattg cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    17761 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac
    17821 cgcgcaccag gcagaaggta tttttcagat ccgcgccaag acacagcaca ggcggaacat
    17881 ttttaaagcc cggaggcaaa accagcgcat ccggcacata cccccgcgaa cggcgcagca
    17941 tttcgccgct ttcgcgcacc actgaatcat ccatccgctg cacgatgtcg cggttatgta
    18001 gcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg
    18061 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18121 gcaaatgctg gagcgggttc gcaggcaaca tcaccccgac ttcgttaagg ccaggggcga
    18181 tatcatcaca aagctcaggt acgtattttt tatccaccag cacaatcggc gcagcgggcg
    18241 tggtaagcaa ctggcgcgca gcgtctggta aactttctgc cactggcaac atgactgcca
    18301 gcggtttcgc cgggcgatgt ttgcgctccc gcagtgtcgc caccgcgtta ctgttacgtg
    18361 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta
    18421 actgtgcgat agctgcctgt aatgccgcct cttgttctgc atgttcacca tgacttaccc
    18481 attcaagatg cgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    18541 gcgggtcgcg gtactctttg tcacaggccg gacatagcgg aaacgccgcc atcacggtaa
    18601 acgggcggtc gtaaggcatc gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt
    18661 tgataaacgg ataacgataa cgccgctcgc ctggggtatt catttcggca aggcaagcag
    18721 ggcaagtggc ggcatccggg acaatttgcg tattcatggt gccgccggtg ctctggcgga
    18781 tggtgaactc ggtgggcagt tgtgaccaga taaacggctc acgctcgacg ctatcaatac
    18841 gcgccagtgg cggacagtgc tgatgcaatt gaacaagaaa cgtttccgga tcttccagca
    18901 gccggacttc tacgccatcg ccgtcattac atacatcgcc gtgaagattt aattgctgtg
    18961 ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19021 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19081 ccgccctgcc ggacacgaca gcgtcgtatc cggcagtctc aggtcggcga taccgcccgc
    19141 tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc tgtaagattt
    19201 ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc agttgttcaa gtttattacg
    19261 atcgacacaa atcagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcacg
    19321 atggttgcac aggtcgcatt tattggcttc ggctttgtca gcccgtacat tcagacccgc
    19381 gccgctgttg cgaatcacag gacgtaccac cacttccatc gcgccatacg ggcaagccac
    19441 cacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc ctttatcacg
    19501 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcaaac
    19561 tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa atgtttccgg
    19621 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    19681 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    19741 caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat ttgctgttat
    19801 ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc atcgacacta gtgacgatga
    19861 catgtgatga caatgtttat cgcgaaaggg caatgagtga gtcgcggcgg atcagttttc
    19921 cgctaaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa
    19981 taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc ggaacgatat
    20041 agggggcaat tgcgatatca tcgaatccga taactgacac ctgctctggc accgctacgc
    20101 cgcgctcgtg caacgctttg atcgcaccta tcgccatatc gtcgttactg gcaactaacg
    20161 cgctaaattt agccccacgt tcgagcaacg tttctacccc ttcggccccg ctggcgggcg
    20221 tccatttacc gttagcgata agtttttcat tgagcgcaat accatgctgc gacagcgcgt
    20281 ctttataccc ggcaagacgt tcaatgctgg tgggggaatc catcgagccg gtaaggaaag
    20341 caatctcctg atgcccggcg tttatcaact ctgccacggc gttaaagctg gtctgtttat
    20401 gatcgcacca gacgctatgg ctgctattat tgcgcaggcg gcgattaagc accattatcg
    20461 gctgactgtg cgcgtcaatg atgtcatcaa tctcatccac gcttaaaaaa cgcgggtaaa
    20521 tcataatggc gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg
    20581 cgctgtgttt accatctgcc aatagcaact gtcgcccttt ctcttccgcc attcgcgcgg
    20641 catgaaacag taattcacta aaataaatgc cgtgataaag cgtgttggtc actaccagcc
    20701 ccagcgtctg agtactcttc gccgacagat tgcgggccag caaatttggg cggtaactgc
    20761 tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg
    20821 aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg
    20881 tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat
    20941 tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat
    21001 gaacaattaa ctctcttgct tatttagtga caactattca tgattttgtg aaaccggttt
    21061 cttaattccg tttcagcatc ggtatttttc cgtcacgtcg actgataaca actacatcta
    21121 ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg
    21181 tgatagcggc actgggcggc gttgataaca tctcggcagt cacgcactgt atgacgcgtt
    21241 tgcgctttgt tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg
    21301 gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt aattatcggc aatacggttt
    21361 cgcaggcctt tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc gcacagcccg
    21421 tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg gatcctcgat gcgctgatcg
    21481 gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga
    21541 tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc attctgaatg
    21601 tgattggtga cggtgctttc ttcttcctgc cgctaatggt cgcggcatct gccgccatca
    21661 aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta catccgagtt
    21721 ttattgaatt aatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc ctgatcccgg
    21781 tcaccgcggt gaaatacacc tacacggtga tcccggcgct ggtgatgacc tggtgcctgt
    21841 catatatcga acgctgggtg gatcgcatta caccggcggt gaccaaaaac ttcctcaagc
    21901 caatgctgat tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta
    21961 tctggatcgg tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc
    22021 tttcagtcgc cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg
    22081 tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaagga atggtcatgc
    22141 catcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg ccgtggaaaa
    22201 cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg
    22261 ggatttccga accggcgtta tacggcgtgg cgatccgcct gaaacgtccg cttatcgcca
    22321 gtcttatcag cggttttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact
    22381 caatggcagc gccggggcta tttaccagcg tgcaattctt cgatccggcg aatccaatga
    22441 gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt gctgtcgttt atcctcacac
    22501 tgttgctcgg ctttgaggat attcctgttg aggaagcggc tgcccaggcg cgaaagcatc
    22561 agagcgtaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt
    22621 atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt
    22681 ccgtgaaggc ggaaaagggc tgaccaccgt cgatatgatc ccacacggcg agcatcgaat
    22741 ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag
    22801 ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat
    22861 gggattcaag gttttccgta cctcaattgc ctggagccgt ctctttccgc aggacgatga
    22921 aatcacgccc aatcagcagg gcattgcttt ttatcgtcct gtctttgaag agtgtaaaaa
    22981 gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac
    23041 cgaatatggc tcctggcgta accgcaagct ggtggagttt ttcagccgct acgccagaac
    23101 ctgctttgaa gcatttgatg gtctggtgaa atactggcta accttcaatg aaatcaacat
    23161 tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga
    23221 tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccaccaaaat
    23281 cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gtaacttcta
    23341 cccttacagc tgcaaaccgg aagatgtctg ggcggcgctg gagaaagatc gggaaaacct
    23401 gttttttatc gatgtgcagg cgcgcggcgc gtatccggct tactctgccc gcgtatttca
    23461 cgaaaaaggg gtaaccatag ataaagcgct gggcgatgat gagatcctga aaaacaccgt
    23521 cgattttgtc tctttcagct attacacctc gcgctgcgcc tcggcggaga tgaacgccaa
    23581 caacagcagt gcggcgaacg tggtgaaatc gctgcgtaac ccgtatctgc aagtgagcga
    23641 ctggggctgg ggaattgatc cactcggtct gcgtatcacc atgaatatga tgtacgatcg
    23701 ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc
    23761 caatggcgag attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat
    23821 gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg gctgtattga
    23881 tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg
    23941 tgacgacgca ggcaacggca cgctgacgcg cacacgtaag aaatcgttct ggtggtataa
    24001 aaaagtgatt gccagtaatg gggaagattt agagtaagta acagtgccgg atgcggcgtg
    24061 aacgccttat ccggcctaca tagttcgtgc gacatgtagg cccgataagc gcagcgcatc
    24121 gggcaattta gcatttggca ttagtcaaaa atcaaagaaa ccattccggc ttaacgccaa
    24181 atcgggctga aagtgcctta atgtgggata tcgtcaggga tcgttgtcca gacaaaatct
    24241 gactgaccag ggatttagag ccgatttcgt tcttcaaatc cgcataggtc agattatgtt
    24301 gatcaatcag cgtgcgaagt aacgccacgc caacaggcat tgccgcaacg gctttgtcga
    24361 actctgcaaa ctcttggttg ttgttttcat attctgcaat gcggctcgcc agaaagtcga
    24421 taagtggatt ggtgtcatct tcttcaatca aatattcgac cagttccagt gcttcacgat
    24481 aatcatcttc tgagtcacta cctcccagaa aggggactgc attgaccagt tctcttgttg
    24541 ctttaacagc gtgtgcagca ttagcagtca ttctttgttc tctctgtagt aacgtgtcag
    24601 tttgtcatac tcggcgtgat tagttatgtg cttcacaaag aaccgcttat taacaaagtt
    24661 tatgtaggca atcaccccta ggttgttccc acctacatcc agaacgtacc atttgttgcg
    24721 atatttaaaa ttgtcgaggt tgggaaacgc cgttcgcatc tcttctggcg tagaaaaatc
    24781 cgtttcctta ataacccgat acaatgcctg aagagccaga gcgtcgttag gatatttacg
    24841 cgcagactct tcaaatggcg ctttagaaat gatgtgcatt tttaagtttt aacttcctta
    24901 tagtaagtaa acagccacat ctgcgagttc tatccttatg tgttcggaaa ctatagctca
    24961 tcaaatccaa gttcacaata tgtaaacact tgtgcgcgat ataattaccc ccaaaatcct
    25021 catagccact ctgcaaaaag tcagttctgg ttcacccctc aagcacatcc ccctttatca
    25081 agcaacgctg cgtctgcgcc agatcttccg tttgcgggtc gcgcccggcg atgggtttga
    25141 ggacgagttt acggttgggg gtataagtaa aacgcgtgag cgcgggatag cttaacttgt
    25201 caaaccagcc gtgaacacgc tgccatttgg cgtcttttac ctgatacaga ctggttatcc
    25261 agtaaccaga attaaactgc atatccagca attgcgtgtg accattgcct tgcaaatcca
    25321 gcaaatcatc tacgccagtt ttgcttgcgg tgtagaatcc cctgggttca aagacacagg
    25381 gacggccttc gcgcgtgaac gtcataagaa ttaaatgtgc ataaggagcc agaccattac
    25441 cggtacttgg taaccagata accagatcct gaatgccgtt acgatcaaga tcggcgcgat
    25501 agagttgccc tttgcccgct atgtttgcca gcatacccac aggcacagtc caggatttac
    25561 cggcgcggtc tttagcggtt aattgccagt aatcttcttc atttggcatc ttctcaagcg
    25621 tcacgccagc aacgcccact ttcctaaaat gccaggtttg ctcggcatca taaggcatgg
    25681 tcaggagagg tttcagcgcc cgcttttgcg cgtattcgct gtcactcatt ggcagatatt
    25741 cggtaaagct ggtggcctgt gcaggcagca ctaagctcaa taaaaaagcg cacatccctg
    25801 ttctgatgtt caattccctg aattccctga attccctgat ggagaattat tcttcttcca
    25861 ccgctaactg cgcaaatcca ccatttcctt cccagccttc cagtcgttga taaacggttt
    25921 ctaccgcatc tttaatcggc tgggtcatcg ggtagtaaaa gccgacgata tccggctgaa
    25981 tgccgaggaa aatcacttca ccaatgtctt cttttaactg gtcgataagg taattcaacg
    26041 gcatgttatg ggtagtcatc ataaacatct cggcgatatc atccgggtcg atgatgcgga
    26101 tttcgccggg gtttagcccc atatccgtgg cgtcgacaat cagcagtcgt gtcgggcgca
    26161 gttcacggat ggcgacgatg tcgttttccg gtgcactacc gccgtcaatc accacccagt
    26221 tacctttcgg cgcggcggcg cacttttccg ccagcagcgg gccagcgcca tcatcgccca
    26281 tcatgctatt gccaacacag agtaaaacgt cagtcacgca gtctcctcac catcaaatag
    26341 atggcgtttt cctgatgaat atcatgcagc atacttaaca gcattgtact ccattcctgc
    26401 tgctgcgaag tttgcgctgc ccgggcttta tcgaatgcgt tggccagcat cggcacatgg
    26461 ttaatgtcga tgacgatctc gccatatttc ggtacgcctt ccattttgtg gcgggcttcg
    26521 ctgcccgcct ccagagtggc aatccatgcc agatattcat cccatgggca ggtgagcgcc
    26581 gcttccaggc aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc
    26641 tgctgcgcct cggcgggcgt ggcatcgttc tcatcaataa atttacggct cagttgactg
    26701 aacaccacct tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga
    26761 caatctcgtt cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc
    26821 cctgccctaa ctgtgtaagg taatcatcgg caatctgacg accgtaacga taacccgcca
    26881 ggcgacgtgc ttcgcgatcc actttcacgc gcagcggctg caccatatcg ccatgcagga
    26941 tctccgccgg ttgttcatcc agttcacccg gcccacgggc gtgaattttc tgctccagca
    27001 ggccgagcgc cattgcaaag ccgtacagcg tggcggcagg cgttggcggg cagctaggga
    27061 tataaacatc cacagggaca attttatccg taccgcccca cacgcagtag agatcgtgta
    27121 agatcccgcc gctgttaccg caggcaccgt atgagataca gattttcggg tccggcgcgg
    27181 actgccacgc acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta
    27241 aaatatccgc atgacgcggt gaaggaacga ctttaatgcc gaagcgttct gcatcgaaca
    27301 gcggagaaag cgtggcgaaa atttcgattt cgcagccgtt gcagccgccg cagtccacgc
    27361 ggtaaacata ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg
    27421 attcatccac cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc
    27481 tcatcagatg gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca
    27541 tttctggcgc ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct
    27601 gtcaccattg tgcttaagca gcgcaatggc gtagtcgatc tctttctgta cggcgaaagg
    27661 acgattgcag acgcggcagt tgcacagcgc gaagcgggac tgttgcagga agtcttcttt
    27721 cttccacacc gccagttcgt actcttgcga cagtttgatc gccgccgtcg ggcaaacttc
    27781 ttcgcagcgt ccacagaaga tgcagcgccc gagattaaac tcccacgcca gctctcctgt
    27841 ggcgaggtca gtttcaaccg ttaaggcgtt tgacgggcag gcattgacgc aagccgcgca
    27901 gccgatgcac tgctgcgggt tctgctctgg cttaccacgg aagtttttat caaccgcaat
    27961 cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt
    28021 aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag
    28081 ttctttgtac ggcaccactt tgctcttctt cttacgcaca tcgactacgg tcatgcggtc
    28141 ggtacaggag tagcaagggt ccaggctacc gataatcagc ggcgcatcgg aaacggtatt
    28201 gccacgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca
    28261 gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc
    28321 ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg
    28381 accgcccggc aggttatcca gaccgtagtc gatcatattc agcgcggtat agacctcgtt
    28441 gattcgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg
    28501 cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga gaccgctggc
    28561 gcgaaccatc gggccaacgt tactgaaatc gcaggcgatt tccgggtcca gacgaccaat
    28621 gccgacggtg cgttgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac
    28681 ttcacgacgc atttgttgcg ccagctggcg ggtctggatc atgtcatctt tcagcagatc
    28741 gtgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc
    28801 tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc
    28861 ggtaaagtga caggcgaggc cgaggttgag cagatgcgag tgcaggcgtt ccacctccag
    28921 cagaatggcg cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac
    28981 cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag
    29041 gaaggtcact tcgttataac ccatgcgggt ttccgccagt ttttccatac cgcgatggac
    29101 gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg
    29161 acccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc
    29221 gcccagttcg ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg
    29281 ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagacc
    29341 aaccggaatc aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac
    29401 gcgcggcgtc acggacggat actccggttt gttggcgtca acttcgacgc gaaccgttac
    29461 ccaacacttg gtgcccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag
    29521 tttgcgttcg tcgttaccaa acagcaccga cagccagcca ccctgcttgt agtagagaaa
    29581 ctccaccact tccggcagat agttcacctt tacggtgaca gtcagctgat ctttggtctg
    29641 ccaggcgtgg tccagcacga cgcctggaaa tgcctcattc agcgcggcga gataatgttg
    29701 acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg
    29761 aacgctaaaa atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc
    29821 agacgggcca tactgttttc gaacagcgcg ataaccagga cgccgaccac cagtttgacg
    29881 atggcaatca ccagcgccag cagcagtcca ccgacggtga aggtttccat ttgtccccac
    29941 ggaataaaca ccccgacgaa catctgcaac accaccagct gtttcaggct gataccccat
    30001 ttcatgacgc caaagccgct accgctgtat tcggagagcg gaccttcctg caactcctgc
    30061 tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaagtggc gaacgcacag
    30121 gcgcagagcg ccagcaccag cgggatgctt tggctcagcg gccagtgata aacggtgtcg
    30181 gtaatgttgc tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc
    30241 aacatcggtt cgaccagcac gccaagcatc gcttcacggc tcgcaccgat agcggtaaac
    30301 gggctaccgg tatccagacc ggaaatggca aagaagaaac gcgcgatggc gaagagatac
    30361 agtaaggtga tcaaatcgcc cagttgcggc agcggagaac cgatagtcag catgacgccc
    30421 accatcacat acggcgtcag gcggaacatc cagccagagg catccgggcc aacgctctgg
    30481 cggcccagca gtttgataat gtcgcgatac tcctgcaaca cgcccggccc gcgacggtta
    30541 tgcagacggg cgcgcgccac gcgggtaata ccggagagca gcggcgcaac ggcaaataac
    30601 accagcgcct gaattaacgg atataaaaca ctcattctca ggctcctcgt gaaacaataa
    30661 tcaccaccag taccgccagt tcaaccagcg ccatccggcg gaacagcaac gcactacctt
    30721 cgcactgcca gcccggcacc agcgacaccg gattcagcca tttgcgcagt ttcagcactg
    30781 gcgcaaacgc ctgtttaacc ggcatggcaa aaccgtgagc ggtaatcacc attgattttt
    30841 catggtcgta gccgcacacc caggccgcac cgcgggaacg tgacggcagg cggtcgcctt
    30901 tgcaaatcgc cataatgatg aatggcagca gcgggcaggc aatcagcagc aatgtgatca
    30961 ttggttgaga aacggtggtg ttagcaggct ccagaggcag aggtacagca gcagagagca
    31021 tcggcagcag ccacggcgca gcaacaccgc caattacgca gcaaatcgcc agtgccacta
    31081 cgcttacgct catcaggagc ggcgcacagg tggcgttttc ggcttctttg gtacgcggcg
    31141 cgccgaggaa cgtgacgcca tagactttcg ccatacacat caccgccagc gcaccggtaa
    31201 ttgccagacc cacagcgagc agcggcccca gcagacgggc aacaaacgcg ccgctattgc
    31261 tcagtttgaa gaaggattga tagataaccc attccccggc aaacccgttc agtggcggca
    31321 gcgcagccat tgccatcaac ccgactaaca tggcgatgga gataaccggc attttcttgc
    31381 caataccacc gagtttttcg atatcgcgat gaccggtacg gaaccagacg ctccccgccc
    31441 cgaggaacag tacgcttttg aacaggctat ggttaagcag atggtacaga ccaccgacca
    31501 ggccaagagc aatcagcgcc ggttgttcga gcgcgatacc cgttacgcc
//
LOCUS       DADLZM010000033        30707 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLZM010000033 DADLZM010000000
VERSION     DADLZM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.033"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="JNY73_001491"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="JNY73_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HAY8047546.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="JNY73_001492"
     CDS             complement(1899..2636)
                     /locus_tag="JNY73_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047547.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="JNY73_001493"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="JNY73_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HAY8047548.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3584..4093)
                     /gene="traS"
                     /locus_tag="JNY73_001494"
     CDS             complement(3584..4093)
                     /gene="traS"
                     /locus_tag="JNY73_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HAY8047549.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4090..6915)
                     /gene="traG"
                     /locus_tag="JNY73_001495"
     CDS             complement(4090..6915)
                     /gene="traG"
                     /locus_tag="JNY73_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HAY8047550.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6912..8285)
                     /locus_tag="JNY73_001496"
     CDS             complement(6912..8285)
                     /locus_tag="JNY73_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HAY8047551.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8272..8697)
                     /gene="trbF"
                     /locus_tag="JNY73_001497"
     CDS             complement(8272..8697)
                     /gene="trbF"
                     /locus_tag="JNY73_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HAY8047552.1"
                     /translation="MNHQKYQRELMMKEKINDTEPGIKQIEREIERGCDNAKKYFWLF
                     VVFFAAGLIVRNVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLA
                     AASLSPLCEIIFGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="JNY73_001498"
     CDS             complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="JNY73_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HAY8047553.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="JNY73_001499"
     CDS             complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="JNY73_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HAY8047554.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="JNY73_001500"
     CDS             complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="JNY73_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HAY8047555.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9819..10154
                     /locus_tag="JNY73_001501"
     CDS             9819..10154
                     /locus_tag="JNY73_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HAY8047556.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="JNY73_001502"
     CDS             complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="JNY73_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HAY8047557.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10490..11233)
                     /gene="traF"
                     /locus_tag="JNY73_001503"
     CDS             complement(10490..11233)
                     /gene="traF"
                     /locus_tag="JNY73_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HAY8047558.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11226..11483)
                     /gene="trbE"
                     /locus_tag="JNY73_001504"
     CDS             complement(11226..11483)
                     /gene="trbE"
                     /locus_tag="JNY73_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HAY8047559.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11510..13339)
                     /gene="traN"
                     /locus_tag="JNY73_001505"
     CDS             complement(11510..13339)
                     /gene="traN"
                     /locus_tag="JNY73_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HAY8047560.1"
                     /translation="MVFFLSFSVFADSNSDYRAGSDFAHQIKGQGSSSIQGFKPQESI
                     PGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAP
                     FIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRTASITGDW
                     KYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFM
                     NTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLV
                     SGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKP
                     WNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSC
                     ESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAAGKDVAALNGVDV
                     RAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGEFC
                     SRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGITVDELQKI
                     QFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13650..14030)
                     /locus_tag="JNY73_001506"
     CDS             complement(13650..14030)
                     /locus_tag="JNY73_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047561.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="JNY73_001507"
     CDS             complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="JNY73_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HAY8047562.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14674..14979)
                     /locus_tag="JNY73_001508"
     CDS             complement(14674..14979)
                     /locus_tag="JNY73_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047563.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15009..16001)
                     /gene="traU"
                     /locus_tag="JNY73_001509"
     CDS             complement(15009..16001)
                     /gene="traU"
                     /locus_tag="JNY73_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HAY8047564.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15998..16630)
                     /gene="traW"
                     /locus_tag="JNY73_001510"
     CDS             complement(15998..16630)
                     /gene="traW"
                     /locus_tag="JNY73_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HAY8047565.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="JNY73_001511"
     CDS             complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="JNY73_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAY8047566.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(17010..19640)
                     /gene="traC"
                     /locus_tag="JNY73_001512"
     CDS             complement(17010..19640)
                     /gene="traC"
                     /locus_tag="JNY73_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HAY8047567.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(19766..20113)
                     /locus_tag="JNY73_001513"
     CDS             complement(19766..20113)
                     /locus_tag="JNY73_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047568.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(20141..20359)
                     /locus_tag="JNY73_001514"
     CDS             complement(20141..20359)
                     /locus_tag="JNY73_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047569.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(20458..20931)
                     /locus_tag="JNY73_001515"
     CDS             complement(20458..20931)
                     /locus_tag="JNY73_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047570.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(20924..21337)
                     /locus_tag="JNY73_001516"
     CDS             complement(20924..21337)
                     /locus_tag="JNY73_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047571.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(21330..21551)
                     /gene="traR"
                     /locus_tag="JNY73_001517"
     CDS             complement(21330..21551)
                     /gene="traR"
                     /locus_tag="JNY73_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HAY8047572.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(21686..22201)
                     /gene="traV"
                     /locus_tag="JNY73_001518"
     CDS             complement(21686..22201)
                     /gene="traV"
                     /locus_tag="JNY73_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HAY8047573.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22198..22566)
                     /locus_tag="JNY73_001519"
     CDS             complement(22198..22566)
                     /locus_tag="JNY73_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HAY8047574.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINVITARVVPDKNVSDDFMHAVL
                     SNCTTKIVLPAPKVFSSESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKT
                     NLTAELISAVVGSNKKGEGQ"
     gene            complement(22505..23095)
                     /gene="traP"
                     /locus_tag="JNY73_001520"
     CDS             complement(22505..23095)
                     /gene="traP"
                     /locus_tag="JNY73_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HAY8047575.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(23085..24512)
                     /locus_tag="JNY73_001521"
     CDS             complement(23085..24512)
                     /locus_tag="JNY73_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HAY8047576.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(24512..25240)
                     /gene="traK"
                     /locus_tag="JNY73_001522"
     CDS             complement(24512..25240)
                     /gene="traK"
                     /locus_tag="JNY73_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HAY8047577.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(25227..25793)
                     /gene="traE"
                     /locus_tag="JNY73_001523"
     CDS             complement(25227..25793)
                     /gene="traE"
                     /locus_tag="JNY73_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAY8047578.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(25815..26126)
                     /gene="traL"
                     /locus_tag="JNY73_001524"
     CDS             complement(25815..26126)
                     /gene="traL"
                     /locus_tag="JNY73_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAY8047579.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(26141..26500)
                     /gene="traA"
                     /locus_tag="JNY73_001525"
     CDS             complement(26141..26500)
                     /gene="traA"
                     /locus_tag="JNY73_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAY8047580.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(26534..26773)
                     /gene="traY"
                     /locus_tag="JNY73_001526"
     CDS             complement(26534..26773)
                     /gene="traY"
                     /locus_tag="JNY73_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HAY8047581.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(26881..27528)
                     /locus_tag="JNY73_001527"
     CDS             complement(26881..27528)
                     /locus_tag="JNY73_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HAY8047582.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(27720..28103)
                     /gene="traM"
                     /locus_tag="JNY73_001528"
     CDS             complement(27720..28103)
                     /gene="traM"
                     /locus_tag="JNY73_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAY8047583.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            28436..29026
                     /locus_tag="JNY73_001529"
     CDS             28436..29026
                     /locus_tag="JNY73_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAY8047584.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(29323..30144)
                     /locus_tag="JNY73_001530"
     CDS             complement(29323..30144)
                     /locus_tag="JNY73_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY8047585.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(30255..30551)
                     /locus_tag="JNY73_001531"
     CDS             complement(30255..30551)
                     /locus_tag="JNY73_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047586.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
BASE COUNT         7782 a         8132 c         6918 g         7875 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccca caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctgcatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcatttttt ttgttttcat ccagaatatc cttttaacta acagactctt
     3601 ttttgaacag acctattact gagactattg cagagagatt ataacgattt atatctatag
     3661 tataaataaa ggaaataatt acaaacagag ttattgataa aaacccaaat aagaacgtat
     3721 gagcaaagga aactattgaa aaaacaatca caaacgacaa gatataagtt ttgttcttat
     3781 catacataaa agatataact ttcgactcat ctctgaattt tttagggatg gcaagataca
     3841 acatcattcc attagtaatg gcaacaaaaa ggataaaacc cattacgcca ctaaaacata
     3901 tatcaaatcc agcatctcgg atatcaactt tatctgccag caaacgaaga aatgtcggcc
     3961 ataataaaac ccataaaaac acatacaccc ctggttttat gacttgtaat aatggcggaa
     4021 tattaactcc ctctacagag agtacatcct ttagtttctg tgtctccgtt tttatatcat
     4081 agtgagtgat cataatggcc ccttcagtct atccttttta tcatcaacca tttttttcac
     4141 agcctcttct gatggctcag gtattatctt ttgactttct cgctcttcat tatatttatt
     4201 attctgagat aatgcctctg ttttatgatg attttgcagt tcagaatact gccctcttac
     4261 aatattttcc tcgccacgga tactgttctg agtatctccg atattgccac gatattctgt
     4321 gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct gcgttctttg
     4381 ctcaataatg gcctgatgtc cctgatggtc agcaataata tcctgactgc caccacccga
     4441 aggtacgctc tccattcctt taccaatatc gccacgggat tcccgccagg cattatcaac
     4501 accaggctgt acctgttcct gcacaaaaaa ccaggccatc gcccgacgac gttctgcaat
     4561 ctccggcgaa ctggtatttg tcagaatagc ttctgcatcc tgcggtgcgc gtttcatcac
     4621 atactgtgca aactgttgcg acaggtcttc actcatctgc ccgctcatgc tttcagtacg
     4681 ggaggccatt tccgcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
     4741 ctgtttcgct gagttcaggg ctgcagacaa ctggtccacc cgggagtcag cattattgtc
     4801 agtatggctg ccggactcac tgaccttgcg actggtaaag taatcgctgg cttctttaaa
     4861 gtccttactg gctttagcgt caatatcatg gcgagcatca tgggtagctc tgctgccact
     4921 acttgcagaa tgtgaatcat catccgttcg atcaagactt cccttaaacc cgcctctgac
     4981 accaacccca ccaccaacac caagaattcc aggtttaatc ccccattcag catgagcatc
     5041 tgcatatccg ccatacgaga acttagaacc tgttgatgcc agttcctgag tcgcctgctc
     5101 attactgata ttatgcgcct tcgcatagct ttccactgca ctgcgcatac ggctggccat
     5161 catggagtcc tgtgcactca tggtgctgtc agcgccaccg gtgacagaat cactgctccc
     5221 ccggttagaa ccaaactgac tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
     5281 atgcagggca ctttctgctc tgttcgacgc ctcccgggcc atttcctgtt gcgccgctgc
     5341 aatctgacgc gttgcattga taccgaccgg taaacgggac atcgctccgc ttgcatccat
     5401 caccatatta ccgtcacggg tctgtgtggc agttgcgcca ctgccggtct ggtacatcat
     5461 ctgaccaaac gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tttccgtctg
     5521 catgttgccg taggagtaat taccgtcaac cacactgcct gctgcactgg ccgtcggact
     5581 gatagaggaa gaggcgaagt ggctgtacac gctggaaaaa cctgcgccaa ggccttttac
     5641 catcatccat gacaacgggg ggatcatggc tgaaagatac ccggcagtag aggccaggtc
     5701 agagtatttc agctgtatct gagagagttc agacagcacg accggcgcac cattcatctt
     5761 cgcatagaat gtcatggcac tgttcagaat ggcatacaac aacggccagg tctgcagcca
     5821 catcagggca aacacatagc ctttcaggac ggacagtgtc agtttgttga agacagctgc
     5881 cagtaccaga agcgggaaaa taccaatggc aattcccgtc aggatagtct gtaccattgg
     5941 caggtttcgc attgtcacat gaccgataga gacatgcgcc agacgttgct tctccatcga
     6001 tgacgtggtg gccagattca ccaggctggc ggtgtcacca ttacgggcag cattactggt
     6061 gataccctct ttcagggcat ttattgtgac gttctggcgc ataatctggc tggcagactg
     6121 actggagccg tagaaataac tgtaactgtc actgaccagt tgtctgaata acaggtccgg
     6181 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctcccgtctt
     6241 tgtatcgaga ttcagcctgt ctttcagcgt gaccgacgca tccttacagg ttatgaaatt
     6301 attattgttg tcataaacgc cccgtagcgg gctgggtcgg gaaaaaatca gcgtgtaggg
     6361 gtcagcggag gccatcagat cttccagcgt gtatttatga ttcaggtaaa tatcacccag
     6421 cacgcagttc tggacgtagt cctggaacag attgatgatt tccggattcc gggacaggaa
     6481 atcggtgctt ttcacaatca gattcgcccc gaacagcatg cctgtcttgc tgtaggtgac
     6541 gctgtccggt tgggtgaata tcatctcgta gctgaccacc atcgcatgcc cgatacgggt
     6601 cgtaagagaa agtggcatcg ccagaccgac cggaacatta tccacccgat ggactttgac
     6661 caggtcactg ttatcaataa tctgcaccga tgtgcggaca ttaaccagca ggctgataag
     6721 cacaaacacg gccacccagc ccagcaaatc catcacgtta tgccgctgta cccacataaa
     6781 agccacagca acaacagaca gggtgagcgc gattttttca attgaatccc aggtccgggt
     6841 actcataaat gcagcaatgg cgttcaggtt attccgcaac cactcaccac cggcaatcac
     6901 ataaacttca ttcacagtga attccctccg aagtgatagt tgttctggta acggctgagc
     6961 atacgggcgg aaagctgctg acgcatgtag ttcatctgac gttcgacaac cagcagcgca
     7021 tcctgctgga cctgtacctg actctggaaa atggcaatct gttgctgtgc ctgattcaga
     7081 ttctccagaa cattgtccat cacagcctgc ggatagttcc cggtggcaat catggcccgg
     7141 gcctgctgaa gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc
     7201 agctggtaaa cgacgctgtt ggacacaccg agcatctgcg ggtcaatcag atatttaaaa
     7261 acgggaatgg tggtgctgga aataaacccc tgttcccggg cgtcaagcgg agtgtcactc
     7321 actgctttat tctgaatact ggtgagcagt tttgtaatct gtgacttcag ggctttatcc
     7381 cgggcgatgg tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag
     7441 tggtaaattt ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta
     7501 cgcgccggca ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc
     7561 atgataaatt ctttcaactc tttgttgccg tcaaacattc tgtttttttt cagcgcctcc
     7621 catatgatat tctggttttt cagcacccgc tccttgtcct tgtcgctggc tttgtcacga
     7681 acagatgagg actgccctcc gaccgtgcac ccctgacgag atgcggccca gtcggcgaag
     7741 atattgcttt cactgccaat gtcctgacaa actttctgct gagattcctg cgtctgaggg
     7801 aacagccccc ccacaagccc ctgtgccacc tgacaggaac tgaggttcat actgttaatg
     7861 tcacttgcca ttttctgcag gaagtctttt gcggttttta tttccggcac cgttgtctgc
     7921 agggcaaggt caaaaaagta accggcagca ttgctcataa tctgcttaac aaaacgctgc
     7981 aactgatcgc cattaataaa actgaacgag ccgagatagg cgtcgatgcc gccacatccg
     8041 gcattgatat ccggcagcgt catggaaatc agctggatat ttttgacctg ggtgcgggca
     8101 tacagggagc caccataggc atacccggcg gcctgcccct gccagacgcc cggctgtgtg
     8161 gtattggagg cgaagcccag cttgttaaag aactgattca tatcgctgtt cacatccgct
     8221 gacgctgcgg gtgtgaccgt cagtaaagcg gcacaaagaa ccagaagagg tttaatgcga
     8281 agcattattg ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa
     8341 tatccggaca cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa
     8401 accagctgca agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata
     8461 acgaaaatta tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc
     8521 atgcataacg ttccttacaa tcaatccggc agcaaagaaa accacaaaaa gccagaagta
     8581 tttttttgca ttatcacaac cacgttcaat ctccctttca atttgtttga ttcccggttc
     8641 tgtatcattt attttttctt tcatcataag ttccctctga tatttctgat gattcatgca
     8701 gacaggtcat gcggctgtaa tatatttcag acaccgaaat ccatcccaca attgccccca
     8761 caaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc
     8821 tggcgggata aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg
     8881 tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc acctttttcc
     8941 tctccgtata tctgcagaac ggtatccatc cgcgccataa aactggcggc atccgttgcc
     9001 ccctgcaaaa gcggtaatgc ctcaagtgtg ttgacgttga ccaggaaggt ggtcggtgtg
     9061 gccaccggga tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc
     9121 ggaaaggcag tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc
     9181 tgtgccagct gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata
     9241 aacagcacca ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag
     9301 cggggagccg gcttttctgt tctggctgat gtgtctgctg ccggttgtgc aggctgcacg
     9361 gccattccct gcggattcca gagtcgttca atttcatccc gggtggaggc ctgcacacct
     9421 gtggccatca tcaacaaaat gaccggcagt aatttagtga gagacatgtc cgccctcctg
     9481 ctgcccttcc atcatggcct gttgcctggc ttccagtgcc tcacgctctt ccaggctgac
     9541 acgtgcaatc catgcatcca gcacctggta tgcgccgaac agcacaagga tggccgcaat
     9601 caactccgcc agaaaaaaag ccatccaggg tttgctgtaa acaagacggg cgacgatatg
     9661 aaaccagaca cccagggaaa atacccacat tcccgtaata tcaagccggg gtaaagagaa
     9721 tctgcgttta cttatcatgt taaactccgg ttattattta catgagagta ttcatgtctg
     9781 taatattcgc gtttgtgtga tattaatcag aggtacaaat gaaaaagtta tcttttaaag
     9841 aaaaactgga aatagtgaga aacatcataa gagagtcatt atcaggtaat gcggcttttg
     9901 ctttactggt gtattcatta ttgcactccc tccccattaa aacctttgct gatttatttg
     9961 tgacgatact gttatttata gcgattatcg ctgttgtgat atggctattt ctaatcatct
    10021 atatttattt ttgtgagtta ttcagaagtc actggatagc agtctggttt atcctctggt
    10081 ctgtggtaat caacctcact attggatttg gcatttatga caaattagca ccagtcattt
    10141 cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca acataataca
    10201 aacaaacatc cttctgaaac tgtcaaactt cgtgataacc ggtatggtac tgactgccat
    10261 tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca agaaattatc
    10321 tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc tcagcagata
    10381 aactccttta ttatttggtc ctgtaaagaa ataaccaaca acccccacga taaacaggct
    10441 ggcacctgta aaaaaacaca aatcccctgt atgcatatat aacctttggt taaaaattcg
    10501 gtttaaaatc ttcagaaacg ttcaggaact gttttgccag gtcgtcctgt gaaataaagc
    10561 cgtatgataa cgggcgaaca ctgccctgtt tcgggtcaac cagcatcatg gccgggaaat
    10621 atttcacacc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc aacagcggat
    10681 taatcacgcc atccacggaa acgggaataa cactcagacc atacgtatcc cggaagccat
    10741 taatgacctg cgccagttgc ccgtcgatgg ggtcctgccc ccggtaaaaa aacatgatgc
    10801 cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct gccgccagct
    10861 ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt tcgggatgtg
    10921 ccagcatggc ctttttagca ctcatggtga aaagcccggc ctgctgagtc cagtaattct
    10981 gcagccggaa atatttaaca aaattatcca cgccgggata cagaatggct tcgtacagcg
    11041 cccgctttgt tgccgtctgc agcgcggcca gtttctgcat aatatccggc tcctgacggg
    11101 gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg ttataccact
    11161 gccagcctgc atctttccct gacgccggaa aaataaagca gcagagtaac agtggcagta
    11221 atgctttatt catcttcatt cctttcatca ttttttcttt catcatcttt ctgcaggcca
    11281 gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta aaggataacg
    11341 aaagcagcag caagtatccc gtctgccgta gtttctttta ccgtatccca cgaccagata
    11401 attaccggag aaatgacaat cgctgtaatc gaaagccgga taagaaaatc aacaagacga
    11461 ttagaagtga atataacttt catcatttcc cccggttatt ttccatacct tactgtcctg
    11521 cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg ccactgtccg
    11581 ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt ctgtcgaact
    11641 gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc gcactgccaa
    11701 acccgatacg caactgcccg ttacgcccct gctgctggac tatctgcgcc agcttcgaat
    11761 caaactggca gtagctgcgc tttttctgca gacagacacc cagcactttc cgggagcaga
    11821 actcgcccac actcaccgtc agtttgttgt cctttgcctt tcccagcgcc ttttcttcac
    11881 tgttgcactt cgccagcccc acatcctgac cccagccgcc atccttacag cagttactga
    11941 agccggctgc ggccttacgg cagaatttcg cctcacccgt gaaagccctg acatccacac
    12001 cattcagggc ggcaacatcc ttacctgcgg cggcaagcgc agccagctgt gatactgctt
    12061 caccaaaatc actgtttttt ccgctctgtg ccttatcgca ttcaccgtca aggcagaaga
    12121 catcaccacc acagaccatc acttttcctg atgttctgga ctcgcaggag tacgtggcgt
    12181 actcatgcag acaggttccc tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac
    12241 aggccggatt atcgacatat ttctgacagg tgccgttatc tgcggactga gtgacatatt
    12301 tatcccggta tgcccagcat gcctgtgtga tgttccaggg tttaccttcc atcactccgg
    12361 ttttcgtacc acccggctct gaacactcag tgcctgtcag cacgccatcc gctttattaa
    12421 acggacaact ctccacccac tccacccggg gaacccattc tttatcacgg accttcatcc
    12481 tgagtttcag cgtaaaggtg gaagcaccac tgacaagcga ttcatagact ttcctgtcac
    12541 cattcccgtg cgggaggcaa ttaccgttag cagtacagcc actaccggtc agaacctgcc
    12601 cctgtgtcac ctgcaaacct gtcgccccgg acagggggta agtatccgac tgcccgtttg
    12661 ccaggccgac attaaaggtt gtattcatga aggtataacg ggagttcagg aagaaaaaag
    12721 cggcgtatac agacaatgat gcactttcca ctgtaccggt taccggagcc gtgacactga
    12781 acaccagttt cagcccgttc atactgaacc ggaactgtga gtgcggtatc gtcacttcac
    12841 gatattcatc cgtgtatttc cagtccccgg tgatactggc agtccgtgtg cagtactcct
    12901 ccaccatcgt gtcccgctca caggtatagt ttgtgaattc actgcggttt atctcctgcg
    12961 cactgcactg ctgtccggta tttcccacaa tgctgtcagc cctgttcacc acatccctgc
    13021 cggtctgaat gaacggtgca tccggtgaca gaatgtcttt cggcttgttc atgaaggact
    13081 ctgtgatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    13141 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    13201 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    13261 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagca aacacagaaa
    13321 agctcagaaa aaaaacaatt gcacaaagat tgcacggctt tacggaataa ttaaaattca
    13381 gagatctgaa aaaggtattt atcttttctt tcataacctg aaccatactg gagaatgaag
    13441 tgatgtcatc tcaacttcag aactgtgttg attgtggtaa agcattaagt cgaacagcca
    13501 caacgtgcgg gaattgcagc tcaacggacc cttttggaaa aaaacggcgc agcgatcgct
    13561 tttaccagtg gtttgtaggg ctgtttatta cggctattat tattgctggc ggattatggt
    13621 acatggggct tcttgacgta ccgggattac tactcagata acgacttcag taacgatatc
    13681 agtgttctga aatcggcaca taacgagtga cgacaatacc ggttatacca gtaatgaggg
    13741 gtgtcgggct gaagcctgaa acagagctga aacttactgt taaaagcccc cttcaccgac
    13801 catccccatg ccagacgtgg tattgctgat tcagcatcct gccgcagtcg ctgctcaaga
    13861 agggcagcca tttttaccgg acaggcaaag gcaatataag gaggcagctt tgtaacaggg
    13921 aaaccatgtc cctgacagga agcatatgtg cggaagccgt gaatgttcat cgcggccacc
    13981 agggcacgga tctccggatc aatctgataa tacccggcag gtaaagtcat ttttgtttat
    14041 ctcccgggtt attcagcaac gcccgagcca cctgccggca gtcccccgtg gcggccactt
    14101 tctccagcgc ctggccgacc cgcaggttac ctcggataat gtcgtatccc tgactgcaga
    14161 acaccaccag tgccggtaca ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct
    14221 gaacaccatc agtcgcgccg tctttcacca gggacagcac ggcttcagcg gtggtcttca
    14281 ggtcattatt caccatgccc cgcagtgtgg cgggtatacc gtagtgccgg gtttcaccca
    14341 gcatccgttt cagcccctct tcgggaatgg aaaacgacac aaaatacagg gcaccctgcc
    14401 ggggtttccc gtcctgcgaa gcctgctgtt tacgaaccag ctcatccaga aaatggttat
    14461 ctgaacgctg aagggggttt tccagcacct gtttctccgc ccaggctttc agctgatggt
    14521 caggtttctc tcgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt
    14581 caggtgtgtt cacattttct gaagcatgaa cagcaagatg catccctgct gtcagagcaa
    14641 aaagtgcaaa tattttccgg ttcatgatat ttctcacttt ctgctattta caggcatgaa
    14701 ctcatcagaa aaaagataat tgagaaaaac agccataaac gcacatgtaa atgtcacctt
    14761 cagatataat ctgacaagtg tcacttgttc tgatatatct gccttcttcc attcatgaat
    14821 aatttcagcg agaaaactgt caggtgcaaa ataaaataat ataacgggca ccattacaag
    14881 accaaaagga aataatttta atgttctgct tatcagcctg agcagcacag cacttatttt
    14941 cctgaggtgc tgaatatatt tactgtccat tgttgccatt ttcatttcct cttattcatt
    15001 aaaatgattt acaggaatac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
    15061 tttgtgttgg gcggattttt tcccgtctcc cagcgcatca cactgcgccc gaacggatgg
    15121 cactgcccgc tgtccggata catattcacc atctgataac gccagcgttc tttgggcagg
    15181 attggggacg gatattcatt acagacggcg ttatttttcc cgatggtttc cataatcatg
    15241 ccctgacggt gtagtttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
    15301 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
    15361 cagaacagaa catcgagagg catattaaag gcgctggcaa tggcatctgc tgcacaggcc
    15421 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
    15481 ctgtccgtcc aggtggggtc aatttcagaa agataagcga tatccaggtc accaccttcc
    15541 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
    15601 acgtgataga acgccccgtt cacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
    15661 ttaccaaaag ccggcaggct gaagcccagg ttaaccatgc atcccggtga ccgggtgacg
    15721 tccgtcagcg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
    15781 ggcggtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttaccc
    15841 tgactgactt tgacactgcc cagcgagagc gggaaaatac agctccagca gatatccgtg
    15901 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgctgcagg gacatgaccg
    15961 gcgagaagga ataacatcag cagccacagg cttcgcttca ttttctgccc tcctctgccg
    16021 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
    16081 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
    16141 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
    16201 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
    16261 gcgtctggtt aaaaggcaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
    16321 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
    16381 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
    16441 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
    16501 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatcgcccc
    16561 aggtgccaag atcggcggcg gccacactct gcccccatat cagcagggca atcagccccc
    16621 ggcatctcat ggttccgctc tcattcgccg ggcaatatcc tgctggattt cccgggtgat
    16681 atccggagcc ccctgtacca cggcaggcga caccagaatg accgcgtggt gtttctgctg
    16741 ccacgcctgc aggctggctt ccagtgccgt gttaaaacgc gctgaaaggg cttttgactg
    16801 tgcttctgac agttgtttct gactggcgct gtcaaaaaat gcatccacag tctgtttcat
    16861 gttgaaggcg gcagtcaccg gtgcgttcag tctgacaatg ccgtaactga cggctgcgtt
    16921 cagtaacacc attgccaggc atcccggcac cgtccaccac cagtggctgc gtcggggggc
    16981 tgtaacatca gtcgtttttt gcgttgtggt catgcaacac tcctgtattt ctcatgttct
    17041 tccagccagg cttccagcga agccatctcc ggccctgact tcttccacgc cagctgccac
    17101 accgcctcat gaatactcag gccctccctg cgtttctgct gcacaaactc aaaatccggg
    17161 ccgtcagagc tgtacatggc gcggcttaac gggtccacaa acagacggtg ccaggaggaa
    17221 tggttttcca cctgcagcag gaaggaactg aaccactggt ctttggccgc accaaacttg
    17281 ccaatcatgt cgcgctgcag tggctggaac tgatccggga ataactggtt atatttcgcg
    17341 aactccttcg cactctgttt gaggataatt ttgtaggagg agttacccca tgccgcacgg
    17401 gccgcactgg aggccttgtc agagtcaaag tcgacgatgt tctgtgtgat ggtgatatag
    17461 gcaccggtat ggcggcgaca ggtgcggtaa cctttttgaa taaactcacc gaccttgcga
    17521 tttttgaagt ccagcagacg ccagccttca tcaatgacgt tcagtttctt gagagtacgc
    17581 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
    17641 agtgacggac ggtcttccag tccgcccagc tccagcacca ccattttcgc atcatcccgc
    17701 agggacggct catcggagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
    17761 agcagcacaa tcatttcgtc cagacggctg cggatggtcg gtgacccggc atactgctcg
    17821 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
    17881 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
    17941 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
    18001 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgcccccc
    18061 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
    18121 gagtccagca cgctgcggat aagtggctgt atcagtccgg ttttaccggc cccggaggtg
    18181 ccacagaccg ccatgttgta gttggtgtta ttcatcccct taaagaagat atcgataaac
    18241 gccagctggt tacggtaggt gggcgccagc agacctgccg gtgtcagggg gttatccgcc
    18301 actaacggca tcaggttggc cacattaaag ctctctgcgc gctgaaccac accggcctct
    18361 ttcagctgtt taaacagccc tttcccggcc ataaagggca ggcaggtcag gaaattgcgc
    18421 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacggaagct gttcaggatg
    18481 tcctgctcca cttccagtgc cgtttcatta ttgtccttgc agaaggctgt gatgttgagg
    18541 aagtaggaca cgacagaaga ctggccggag cccagccgct ggcgcagttc cccccactcc
    18601 ttcgcctctt tctccacgga cggaaaccat ttggcatatg aggtcttcga cttcttctcc
    18661 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
    18721 gtcagcgtca ggatgaacgg gcaggaaatg gacatttccg ggttcagcag attgctgtag
    18781 ttgtcggcca tgttccacag gaaggcgatt tccgggttac gggccagatg gaaattcagg
    18841 atgcgggccg tgctgttcct gccgttctca cgcaggccca gcgtcaggta atcagcccgg
    18901 acattcaggt caaaactgtc ctccacacac tgataattca gatcagaata cgggtccagc
    18961 tgacgtcttt tcgggtacag agagtccgga ttatggttaa tcatctcccc gacaatctcg
    19021 atgaaagcct gtgcatccac tgtctgtgtg gtgatttttg cgccgtgaaa agaggcacgg
    19081 atgattttca ccagattttc catttccaga atgtcagccc ggcttttttt cttcgaggga
    19141 gaacagtacg agataaacac ccggtaatga cgcagggtca ggggcagatt catcccctcc
    19201 ggcagcggga actgtgtcgc tgccgctttc atataatagg cacgggtaat ggcgttaaac
    19261 cgttcagcct gttcacctga ccaggagaac tcacgcagcc cgtattcaat cctgtcacca
    19321 accagctgac tggacatcag atggatacac aacggaatac cgcgcggcag tttggtgcgc
    19381 agcatgtgat cgagtgcctc cacaatggac ttattcgccc cattgatggg aatggcttcc
    19441 agcataaagc ccatcgtggt gtcattcatg aacagaccgg attcctggtt gtaatcacgg
    19501 tacggcagca gacggctgaa ctgcgggaag ctcatttcgc ccagaacttc attggccttt
    19561 gcggattcgt caggcagttt cagtgctgtg acgagggagt taaccgcctg agtgacggcc
    19621 tcaagtgggt tattactcat atgagtattc cttagtgcgt ttaaaaaagg aaaatatcag
    19681 tctgtggccg tggatatatc acgcagtgcc attcctgaaa ccacagcaca aataagagat
    19741 ggaggctttt aacaggaaac caggattaac gttcctttac tgaatgagca tcatttaatg
    19801 cctgaatttg tttttcgcga atcttattgc gctcattttc tcttacccgt ttcacctctg
    19861 ccatttcgtt tctttttctg cattctgatg cgattttatt tgccttacca agtgttaaag
    19921 gttcagactg ataattaagt gtctcctgaa atgcttctgc tgcacaggga gtttcctgta
    19981 aaatctctgt cagttgttct ggtgttgcag gttgttgcgc aaggtaaacg atgtaaaaag
    20041 gaatacatac aatcatagcc ataacgccaa caaatataaa agccagaaca gattttaaat
    20101 atatcgactt catttcattt tccttcgaag cagtaagcac tcattgccca ccaccattta
    20161 gtgtttccat cacgctgttg aaaggttcat ttgttttaac ttcaatacac tcccctgtat
    20221 ttccaaagcc cctgccgcca tgtgcttcga ttgtccgaag aacctgcacg catacgtccg
    20281 tatttggcat gggtgtaata ttccagcttc cgccaccgtt aatatttttt atcagcacaa
    20341 gaaaaataaa ggtatgcatt ctttattatc tccgcttgta tattaatcac tgaatatccc
    20401 ttcagttaac ttatgaggtc ataaaacact gcagattttc attcagattt actctcttca
    20461 gcaaacttta ctctcttcag caaacttccg gatggtatcc tgttcacgct gacgggtggc
    20521 agactcctgc attgcatcca ctttctcctg ccagagacgc cggatatcct tttcatcccg
    20581 accagtcagt tttttaccgg acagcagacg gttaagcagt tcctgtctcg catccggtac
    20641 gtcttcagtc agacgcataa gctgaagcag gctctgacgg ctgagaacca tgagtgattc
    20701 cggcaggagg gtgaagaaga aaataaaaaa gcataccgca ccacagcagg caaggaccgc
    20761 aaacaggatt ttcagatata taccatcaac gccgaacgca gcaataatac agggaatcca
    20821 tgcaatacac gcacaatacg ccaggatgtt atctattcgg ttatgtctgc gatatatctg
    20881 ttgatgttca tccagaatag aatctatttt ttcagcttct gatttatgca tagtgtttcc
    20941 tctgcgtttc ctgccagaga cgccggatat cctgttcatc ccgaccagtc agttttttac
    21001 cggacaacaa acggttaagc agttcctgtc tcacatctgg tacgtcttca gtcagacgca
    21061 taagccggag taattcctga cgactgagta ccatgcgtga ttcctccggt acgctgtaat
    21121 aattaaaaat acgactgaat accccacaac agcaggcaac ggacaggcac agggatttca
    21181 gatacaggcc agaaacacag aatgtggcaa caccacaaag aatagctata actaaaaatc
    21241 ccttacccag tgccatacac aaagaaccct gttttctgga tatccgctca tgctcatcca
    21301 gaataaaatc tattttttca ttttctgatt tatgcatagt gtttcctctg cctttcctga
    21361 tacgcctgac attcaacgca caacgtcaca ccgggaaata ttttccgccg ggcttccgga
    21421 ataggatttc cgcatgcttc acagagataa acaggaatac catgaacatt tatttcctgg
    21481 cgtatccggt ttattcctgt catggtcagt tgttctgtca ctgaatatgc ttcatcggct
    21541 tcatcactca cggaatattc ctcccgacat gttctgacac gctaccgccc atcccttttc
    21601 acgcacaggg cactcgtttt acaggcggat tttgaataca gaaagcccgc cggcattgcc
    21661 ggctgatata caacttcagg gccagttaat taatacgtgg ttttccccac gcagacggtt
    21721 taataacaaa aaacacgctg gatggctgat gataaacatc ctggttatca atataaggcg
    21781 ctatccataa tgctgccgtc tgctcacccg ttcttagcgg acgaggaggt gttaccggcg
    21841 taactgaact gaccggaaca accgttttca cttcccgggc agcggtaaac agcgggcgtg
    21901 gtgccagcag tttctgctcc ggatgtgctg tcaccgcagg tctgctgccg gacggagtgg
    21961 tcgcggtgac ggtctgcacc ggcattgtcc ggaagttccc ttcagccagg cgcggcagtg
    22021 atgccgcaac cggctttgct tctgaggact gctccagttt tttggccttc tcattggcct
    22081 gctccatcgt catacaggta tcggatgtgg tggcgttaca ctcaaattcc gtatttgttc
    22141 ctgcgcagcc ggaaagtaat aaggttccca gcagaggaat aaataaagaa atctgtttca
    22201 ttgcccttct ccttttttat tactccccac aacagcagaa attaactctg ctgtcagatt
    22261 tgttttccct gcacagggat gtccggagac cactgtattt cccggagaag aaaataagct
    22321 catcgacatt cccttcttca tcacaccgac agcagccata ttaaatttgt gcggcaatga
    22381 ttcagaactg aacaccttcg gagcaggaag aactattttt gtggtgcagt tactgagaac
    22441 agcatgcata aaatcatctg atacgttttt atccggcaca acgcgggctg taataacatt
    22501 aatattacgc atgttcattt ttatctccgt tgcttctgtt tgttcggtca tcagggaaat
    22561 aaacatggca tttcctgatt tttcccggca gggaagcgtc ttccgtataa acgtccttca
    22621 gatattcatc tgcggttacc acccggatac atttcatttt tccgggcagc accggatatt
    22681 tcccggtgat cgctgccagc gagacataca tcagtgccag tgttgcccat aatgcggtga
    22741 ccagttgcat cagtgaccgg tcagtctccc tgacataatc tgcagcatca gccgcgcggt
    22801 aatggcagat atcctgtatt tctgatctgc ctgaagaatc agaggaagaa aaccagcatt
    22861 cactgaccgg gaactgtccg gcaggtgctg tttgtcttac gaagttaatc tccttaacca
    22921 gtaatttccc cggcgtggtg ttgtccttcc ataaaacaaa cagagccatc agggccattg
    22981 tggccagtgg cagaatgacg gtgtacttca gacaccagaa cagtcctcgc agaacccggg
    23041 cgacaacata ccgggcggca tggcacgcct gacgggacga catattattt gccatcgttg
    23101 cctcctccct ggctgccgga cataaactgc tgcggattca ggttgcccag ctgcttcagc
    23161 atctggtcag tattaaagcc gttaagatga ctttcagctg atggcggaac aggaaccgga
    23221 ctttccggat tatcttcttc cgctctgttc tgcgtgcgct ccagcgccat ctcttccacg
    23281 gttttcagct ggaagccgtc ctggaacacc acggtcactt cgttgcccgc accaatcggt
    23341 atcaccgggt gatactgttc ggcacgtttg atgtagtagt cactgagcgt ctgtgcggct
    23401 ttcgatgtgc cgccaccgat acccattttc aggacatcac cagccccgta agcggctgtg
    23461 gcacccagcc cgacagccgg ctgggaggcg cgctccattc cctgaccgat accgtcaaca
    23521 aatcccgcgc cccatgccca gccgaggatt ttgccgttac gcatcaccac ttcgcccttg
    23581 ataccgtttt taccccggaa gctgacatgc cccttaatcg gcatatcaat agttttgccg
    23641 tccttcaggc aactgatatt gcgggtgcgt acaatggcac gctcactgga cacatccccc
    23701 caggcttcca gccccacaaa acatcccgtt gcgtcatacg tcttgctgtt cggcatttcc
    23761 accaggccgg tgatacgcaa ctgcatcggc accgtggatt cattaccggt gactgaggca
    23821 ttggcatccg ccccttcaat cagcatggct ttcgcaaaac ttcctgacgg aatgtacggc
    23881 agcgatggtc cctgttttcc ctcattacgg gtaaacacct tacgctgtat ccggttaggc
    23941 accggcacag actggtacgt cacctgtggt ggtggcgtga caccattccc gggataaaac
    24001 gccgtcggcg gtggaacagc aacgctgccc tgcggcggga aggatacggg ggtgtttcct
    24061 ggctgtggtt cgccttccgg cccgggcggt gaggcaggca tctgaggtac aggctcaccg
    24121 gtcgccgtga cgggattagc acccagggct tttacctgct ctgccagggc ggcattgtcc
    24181 tgtcccagct tttcaatacg acgctggtca tcaccgcgct gtttgttcag gacatccagt
    24241 tcacgacgga tttcctcata ctgcttctgc atctgcgctg ccgtcacctg catctctgtg
    24301 gtggcatgct gacgcacctt gtcatcaaag gtcgtatcca ccacacctgt catatccggc
    24361 acaggctcct gttcagccac cgcctcaccg ttaccggaca tgtccacatc agacagatac
    24421 agtaccccac caatcgcgga ggctgcaccg acaaccacaa tccccagcca caggtactgc
    24481 ttgcgtttca caatggtatt gatactggcc atcagttgcc ctccccgtca cggatgacat
    24541 acacatccat gcgcgcacct gccagtaact ggctggcagg ctggctgaac atcacggcac
    24601 gggttcccgg ctgccagaag tcactttccc ggatattcag ggcagacagc gttttatttt
    24661 ccacggcaaa gcggaccatc ttcaggtgat tccccgtcca tacggcatct gccactgaag
    24721 acagccccgc cggggcctgc agggtttcct ttgtcacagg aacctgatac cagcctgcag
    24781 gtaattttcc gccacggaca gcctggctga tggtcacaag cagggattcg taaggcgtgg
    24841 acgtttccca cgctccggct tcttctccgg ttccgcgcag gtcactgacc agctgaatgg
    24901 tacgccccgc gccttcacgg ggaacagcct gaatggaaag gttcagacca cgctctgttt
    24961 ccagaatgaa cgtaaagggc tttttgttga cggtggccac caccacaccg ccggaggcgg
    25021 tctgctcatt attggtcagg gcaccatcca gactgttcac ggcgataatc cggtcaccgg
    25081 gaacggtaaa cagattcggg ctggtattgc tgatggcaat actggcctga ccaccgttca
    25141 ccattggcac cacagtgggg gccagcgtac cgtttgcggc catcacgctg caggaaaaaa
    25201 acaggccgcc gaatattatt gccgtattat tttttctcat cgtctgtttc cccgaaatta
    25261 tccagccagg tcaccccgtt ttcccgcttc agaataagga tgtaatgttt gatatcagta
    25321 aaaggttcgg agttaccaat ccatgttttc cgaattccac ggatttccac acgaccatac
    25381 tgaggccaga cacggacact ggtctggaaa aaagcagaat tcacgttatc ttttttaatg
    25441 agtttcgctt cttcagccag aataactttc atctggttct gtgcacccgg gcggatatat
    25501 tgcagaagcg cctgatgtga agcatcgacg atttccggtg agacattcag acggagggca
    25561 ataaatgaca gcgccatctg ctgcaaataa gaggcatcgg cactgttctg tgacacggca
    25621 aagggcacat taaatcccat tggggtcacg gcagtgcgtt gctcattctg aagacggtag
    25681 ttattcaccc cctgaatgag gttaacagag aggctgagaa caataagcac tgacataaat
    25741 ataaaggcga tggccattac acgactggta cttaaacggg caccgtgttc catataacaa
    25801 tcctggtatc agttctattt aatccactgc cggaaacacg aatcgggaac attatgaaaa
    25861 ataccgcgca gcagggctgt tggcatatac cagtaaatca ggtcacgtaa ccaggaactg
    25921 ccccgccctt ttttcagttt tttaatcccg aaataaacca gaaccgctgc accaataccg
    25981 aacagatatt tcgatgttgt gataccccag ccaacacaga ttgctgcggg gatcagttca
    26041 tccagtggca ggccaaacca gcggctctgg ttggtcagtg tttccgggaa acgatatttc
    26101 ttaagtttat tctcgtctcc cgacatcgtt ttatttcctg tcagaggcca acgacgctca
    26161 tacccacagc aataaataca gagatgatgg caaagccgac caggaacttg acgtttttgg
    26221 tcatcatgta catgaccgca ccgaccagca cctcagccag aacaacccat ttaacgatac
    26281 tggagtcctt accgaaggtc gccttaaccg tatcgttacc tttagccatc aggtcgcctt
    26341 taccagcagc catagctaac tcagggaaca acatcatcag aacagcaacc gggatcactg
    26401 cacgaaccag gcgaagcata ttcagacgag tgaatttaga aaaaaacgat ttattttcaa
    26461 cgggcgcaga agcaccctga acacttaaaa cagtattcat atgattcctc tgattaatat
    26521 tgacagatga aaactaaagt tctttaaatg ttgactcgtt ctcttcgatc acctccctga
    26581 atatttcttc attataaaaa tctggataac gttgcaaatg atcctgcaac ctcaccaatg
    26641 cttcgtttgt tttcgttctt ccactacgct ctctggctcc aagtaataat ttattcgttt
    26701 cctcatcaag ggtaagtaaa acttttctac ttggcgcaga tcttaaagct cctttaatca
    26761 ccctccctcc cataaataaa agagatttag tcaaaatatt cacataagac gcacacaaaa
    26821 ataaacaaca aaatagtaaa ttacgttaac aaaaaccggc acttttagtg ccgggaatgc
    26881 ttacatttct ttacagtgaa ggctgttaat attactagcg aagtattgat atagataaaa
    26941 taacacatca gagattatag catatcgcaa ccactcatcc ctgtctgaga aaccatagtg
    27001 ttttttaaaa ttcataaata tttttttaga ttggtcaaca gagatatcca ttctctgaga
    27061 agaatactca tgactataac ccaaaagata cggtattatc ttattaaagc gatatttgtc
    27121 aggttcacaa ctaaactcct tgccctttat aaaaaagcca ctaactacgt atctttcaaa
    27181 gaatctccag acaaacaaat tcaagttatt cacttccgta ctttcaacca ccacatccca
    27241 tgatacatca ttcaatatta caccattaat aatactcact ccattcgagg acgagaaagc
    27301 atcaacttca gcagtatgaa ataaaatttg cgtatcaata tcaataccag aaaaccatat
    27361 ttcagaatca ctattctgac actttattaa gtgtaaaaaa acagagtttg agtctatgaa
    27421 ttttcctgac tctgtgcgga cacagactgg atattttgcg gataaaataa tttccggcag
    27481 gcctgattga cgtatgagtg ctctttctct agtatccatc gcacacatag gaacctcctc
    27541 aaaggattct atggacagtc gatgcaggga gggacaagct ccctgcatcg gcgattttca
    27601 agttcaaatt ttaacgtggc attaaccacg taattaatat actaattatt ctgattattg
    27661 tcatcagttt gaaccattaa aaagtctgat atttgaacaa agtcaaaatt tatttccctt
    27721 tattcatcat cattttttgg aaagaacctt tccatctcag ccgacacctt ctctctgata
    27781 tcttcaacca tattggcata ttcaaacttt ggatttcctg agacatgagg actgagagac
    27841 tcaataccca aaattttcgc tactgatgac tgtgttttta caacgcattc aagaagtagt
    27901 ttattaaact cggcctgatt aaatgcagac tctttgcgct ccatctgagc ctcatataca
    27961 cgaagaccca gttcaagaag cattgaagcc gttgctgaaa aactgacatc tttttctctc
    28021 gccccctcct gtcgacgttt ttcaataatc gcgtttattt tttcataagc atcattgctg
    28081 atatacagat tcaccttagc cattagatat tacctttttt aaacataaaa caaagttaat
    28141 ttcgaaacac tagaattcat agtgcaaaca gggacgcacc gctagcagcg cccctgacgg
    28201 tatcctataa aaaaacacac cgcgccgcta gcagcacccc taatataaaa taatgttttt
    28261 tataaaaata gtcagtacca cccctacaaa gcggtgtcgg cgcgttgctg tagctgcgtt
    28321 aacgacgctg ctttaaaaaa atcagattta aacaatataa atccacaaat acaactcaat
    28381 gatattaaag ataaatcagc aaaaacttgt ttttgcgtag tgtgtggtgc ttttggtggt
    28441 gagaaccacc aacctgttga gcctttttgt ggagtgggtt aaattattta cggataaagt
    28501 caccagaggt ggaaaaatga aaaaatggat gttagcaatc tgcctgatgt ttataaatgg
    28561 gatctgcgaa gccgccgatt gctttgatct tgcaggtcgg gattacaaaa tagacccgga
    28621 tttactgaga gcgatatcat ggaaagaatc ccgttaccgg gttaatgcca tcggtattaa
    28681 tccggtaacg ggatatggca gcggactgat gcaggtagat tcccagcatt ttaacgaact
    28741 ggcccgctat ggaattaagc cggaacatct gacaacagat ccctgtatga acatttatac
    28801 cggtgcttat tatctggcaa tagcctttaa aaaatggggc gtctcctggg aggccgttgg
    28861 tgcatacaat gccggattca ggaagaccga acgccagaac cagagacgtc ttgcctacgc
    28921 atcagaggtt taccggattt atacctggat aaagagcagt aaaggcatcc gggttccgac
    28981 cacgaagaaa tcactttccc aaattaacag tgtgcagaaa aattaatatt gtatacattg
    29041 tcaaataccg gttatgccgc gacagcggca tggaaggcca cacacaaaaa tatcatatga
    29101 gtgataatgc tgactggccg aagccccgca aagcgggatc ccatcttata tatatccggt
    29161 aactgcgcag cctcctgcca gaccattttt cgcgggcaca atatttacag actaccgaaa
    29221 agccagttga tgaaattgag gcatatcaga cgacacagca atgccgcagg atgataatat
    29281 aagtaaagcc ccgaaaaatt tttcggggct ttacttatat tatcatgact tccactccct
    29341 gaactgttcg gcaatcatcc ataatgcttt gttgattcgg atatcaccgt caatgcctgt
    29401 aatggctctt gttctggtat ttttaccgga ggcacttcgc cccgataatc caccttttat
    29461 catattctcc tgaacccgct gccaggttgt ccagagatcg ttctgtttat cctcccaacg
    29521 gcggggagta attatttgtt ctggcgtcac tggcgtttta ttttcatctt cgtacctggc
    29581 catcaatgca gctctgccaa ataaatgttg ttcgtcactg ttaagatgta tctctttcat
    29641 tgtctccata ttttcagtga ctttatcaaa gacaccgagc acttcatacg ctccctcaat
    29701 aacctgcccg acaatatcac ctttatgtgg aacgcggatt tcgccaaaat tattcccaca
    29761 caccaggcca tttgtgcaga caaaacgaaa aattcctggg atcatctgat aactggatga
    29821 accatcatgt gaattaagca ggataatttc aggaacttct ttcccgttaa tatgcccttc
    29881 cctgcgaaga cgtaacatat gtttactgta ttcgcggcgt cccaaatcac gaacccgact
    29941 ctgacaggca aagaatggct ggaaaccttc atcacgtaac ttattgatga tattgatggt
    30001 tgggatatat gtataacgct cactccggga ttcatgttta tcaccagaaa agacagatgg
    30061 aacaaactgc attaattcgt catccgttaa cgggcgctca cggcgaatag aattatatcg
    30121 accaaaacgg gaagctaatc gcataacagt aactccttta tgaaggcata aaattaataa
    30181 aagttttttc gcttaatatc atcccccctc ggttatcgtt aacaaaaaga caacaattcc
    30241 gaggggaagg accatcaaat caggtcagtt cgggactgac caacctgccg agtgaaaagc
    30301 agtaacagcg cgatcaactc atcagcgttt tttctgacaa gaggagtatg attctccagg
    30361 ctatccagta agtcatgcag ctcattattg acctggtgga gcaccttaaa gagtgcacca
    30421 gaatcccggc agctctgata tgcaagattt gcctgaacag cacgattctg catgatcccg
    30481 atttgtgcga ttgcgtcttt ctgctgatcg cctgttggct gataagtggt gttaaaagaa
    30541 aatgcgctca taattcctcc tgtatctgat gttttcagcc cccatcatct gttgagtttc
    30601 gggagccgcg gttttgacgt tttgcgggga cctgcctttt gttccctccg gtattcacca
    30661 tcacctgttt gagaaccttc gccgcaaggg gcatcactgc cacggcc
//
LOCUS       DADLZM010000034        30553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLZM010000034 DADLZM010000000
VERSION     DADLZM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.034"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..920)
                     /locus_tag="JNY73_001532"
     CDS             complement(<1..920)
                     /locus_tag="JNY73_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY8047587.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKV"
     gene            1215..1889
                     /locus_tag="JNY73_001533"
     CDS             1215..1889
                     /locus_tag="JNY73_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8047588.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(1928..3589)
                     /locus_tag="JNY73_001534"
     CDS             complement(1928..3589)
                     /locus_tag="JNY73_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY8047589.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(3785..4213)
                     /gene="ibpB"
                     /locus_tag="JNY73_001535"
     CDS             complement(3785..4213)
                     /gene="ibpB"
                     /locus_tag="JNY73_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY8047590.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(4325..4738)
                     /gene="ibpA"
                     /locus_tag="JNY73_001536"
     CDS             complement(4325..4738)
                     /gene="ibpA"
                     /locus_tag="JNY73_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY8047591.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            5043..5375
                     /locus_tag="JNY73_001537"
     CDS             5043..5375
                     /locus_tag="JNY73_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY8047592.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(5377..6550)
                     /locus_tag="JNY73_001538"
                     /pseudo
     CDS             complement(5377..6550)
                     /locus_tag="JNY73_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            6615..7633
                     /locus_tag="JNY73_001539"
                     /pseudo
     CDS             6615..7633
                     /locus_tag="JNY73_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(7594..8931)
                     /locus_tag="JNY73_001540"
     CDS             complement(7594..8931)
                     /locus_tag="JNY73_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8047593.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(9006..10154)
                     /gene="dgoD"
                     /locus_tag="JNY73_001541"
                     /pseudo
     CDS             complement(9006..10154)
                     /gene="dgoD"
                     /locus_tag="JNY73_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(10151..10768)
                     /locus_tag="JNY73_001542"
     CDS             complement(10151..10768)
                     /locus_tag="JNY73_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY8047594.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(10752..11619)
                     /locus_tag="JNY73_001543"
                     /pseudo
     CDS             complement(10752..11619)
                     /locus_tag="JNY73_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            complement(11616..12305)
                     /gene="dgoR"
                     /locus_tag="JNY73_001544"
     CDS             complement(11616..12305)
                     /gene="dgoR"
                     /locus_tag="JNY73_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY8047595.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            12583..13239
                     /locus_tag="JNY73_001545"
     CDS             12583..13239
                     /locus_tag="JNY73_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047596.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13285..14097)
                     /gene="yidA"
                     /locus_tag="JNY73_001546"
     CDS             complement(13285..14097)
                     /gene="yidA"
                     /locus_tag="JNY73_001546"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY8047597.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMGNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(14211..14618)
                     /locus_tag="JNY73_001547"
     CDS             complement(14211..14618)
                     /locus_tag="JNY73_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY8047598.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(14849..17263)
                     /gene="gyrB"
                     /locus_tag="JNY73_001548"
     CDS             complement(14849..17263)
                     /gene="gyrB"
                     /locus_tag="JNY73_001548"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY8047599.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(17292..18365)
                     /gene="recF"
                     /locus_tag="JNY73_001549"
     CDS             complement(17292..18365)
                     /gene="recF"
                     /locus_tag="JNY73_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY8047600.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(18365..19465)
                     /gene="dnaN"
                     /locus_tag="JNY73_001550"
     CDS             complement(18365..19465)
                     /gene="dnaN"
                     /locus_tag="JNY73_001550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY8047601.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(19470..20873)
                     /gene="dnaA"
                     /locus_tag="JNY73_001551"
     CDS             complement(19470..20873)
                     /gene="dnaA"
                     /locus_tag="JNY73_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY8047602.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            21170..21250
                     /locus_tag="JNY73_001552"
     CDS             21170..21250
                     /locus_tag="JNY73_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047603.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            21480..21620
                     /gene="rpmH"
                     /locus_tag="JNY73_001553"
     CDS             21480..21620
                     /gene="rpmH"
                     /locus_tag="JNY73_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY8047604.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            21637..21996
                     /gene="rnpA"
                     /locus_tag="JNY73_001554"
     CDS             21637..21996
                     /gene="rnpA"
                     /locus_tag="JNY73_001554"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY8047605.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            21960..22217
                     /gene="yidD"
                     /locus_tag="JNY73_001555"
     CDS             21960..22217
                     /gene="yidD"
                     /locus_tag="JNY73_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY8047606.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            22220..23866
                     /gene="yidC"
                     /locus_tag="JNY73_001556"
     CDS             22220..23866
                     /gene="yidC"
                     /locus_tag="JNY73_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY8047607.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            23972..25336
                     /gene="mnmE"
                     /locus_tag="JNY73_001557"
     CDS             23972..25336
                     /gene="mnmE"
                     /locus_tag="JNY73_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY8047608.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            25578..25652
                     /gene="tnaC"
                     /locus_tag="JNY73_001558"
     CDS             25578..25652
                     /gene="tnaC"
                     /locus_tag="JNY73_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY8047609.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            25873..27288
                     /gene="tnaA"
                     /locus_tag="JNY73_001559"
     CDS             25873..27288
                     /gene="tnaA"
                     /locus_tag="JNY73_001559"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY8047610.1"
                     /translation="MENFKHFPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            27379..28626
                     /gene="tnaB"
                     /locus_tag="JNY73_001560"
     CDS             27379..28626
                     /gene="tnaB"
                     /locus_tag="JNY73_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY8047611.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWSAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGARLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            28758..29933
                     /gene="mdtL"
                     /locus_tag="JNY73_001561"
     CDS             28758..29933
                     /gene="mdtL"
                     /locus_tag="JNY73_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY8047612.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            29908..>30553
                     /gene="yidZ"
                     /locus_tag="JNY73_001562"
     CDS             29908..>30553
                     /gene="yidZ"
                     /locus_tag="JNY73_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY8047613.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         7346 a         8092 c         7740 g         7375 t
ORIGIN      
        1 acttttaccc gattttccgg tgcggcagtg aagtacatcg ctaaagaaat gcccacggca
       61 ccaaagattt ttgagttacc gtgcagggca aaaccgcctt ccgggaacaa cgatttcagc
      121 ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc agtacatctg aatgccgcct
      181 tcaacagctg ccggaccaaa gataaacggt ccgtagatga agtggtgtaa accggttggg
      241 atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa gcgcacccgc cgaacgcagg
      301 aacgcttgca gagattcaat ccccatttgt acttttggcc agccgagcaa tgtcagccag
      361 gcacagggga tcatcaccag gaaggcgata atcaccacat aagacgtccc ctggaaaatg
      421 ccgagaaaaa ccggcagttt tttatcgaac agacggttat gcagcgccgt cacaatgccg
      481 gaaatgataa ttgcgccgat aatgctggta tcgagggttt taatcccggc catcattgtc
      541 agaccactac ctgccaccgc gtcctgagtg aaatcgacgc cgaagtagct tccccaggtc
      601 attcccatcg cattgatgaa atagttccag gtcaggaaac tcaccatcac cgccagacaa
      661 gcacgtccct gcgcttgctt agcaaggcca atgggtaaac cgacagcaaa aatcagcggc
      721 atattacgga ataccgtcca accgccctct tcaataatgt gtacgatttg cgcgaataaa
      781 ctgttcggat cggtcagtga ttccccgaca aacatcgggt tttgcagcaa gatggcaaga
      841 cccaccacaa tcccggcgaa gggaaacagc agcactggcg tgaacatcgc gccgccaaag
      901 cgttgaattt gactgagcat ttccacatcc tcttttctca attctgtaag taggaagcct
      961 gattggttat gcggcagacc tggagcgagg ataggaagat gcgatcggca gaacatgacg
     1021 acccaatggg attcgtgatc gctttcatgc ttttcgttgg gcggtagaga tctacgtttt
     1081 cgcgaaaaag tagacatctg tgagcacagg tacacagcat cgaacacatc tttaaaaaaa
     1141 agattttttt caatcgatta agcagaactt gtgggcgcat tacccgtgct tgcaggcaaa
     1201 aaagagagct agagatgatc tacaaaagca ttgcggagcg gttaagaatt cgacttaatt
     1261 ccgcggattt cacgctaaac ggccttcttc ccggtgaaaa aaagctggcg gaagagtttg
     1321 cggtatcgcg gatgactatc cgtaaagcca ttgacctgct ggtagcgtgg gggctggtgg
     1381 tccgccgcca cggcagcggc acttacctgg tgcgcaaaga tgtgctgcat caaaccgcca
     1441 gcctgaccgg actggtggag gtgttaaaac ggcagggaaa aacggtcacc agccaggtgc
     1501 tgatttttga aatcatgcct gcgcctccgg ccattgccag ccagttacgg attcaaatca
     1561 acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg ctaatgctgg
     1621 aagacagcta tatgccggta aaactgttcc gtaacctttc actgcaacat ctggaagggt
     1681 cgaagtttga atatattgaa caagagtgcg ggattttgat tggcggtaat tatgaaagcc
     1741 tgatgccggt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg gaacacacgc
     1801 cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg aattattcag
     1861 tgatgttcag aaatgccagc gaatactagg tggagtacca tttacggcga ccccacccgg
     1921 aaaagagtta accgatactc cagaagagca ccgccagtaa ttggggggtg ataatgcgca
     1981 ggaacatcac taacggatag acagtggcgt aagagagcgc cgccgcacca ctggttggat
     2041 gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc agcatcccgc
     2101 acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca gtaatcagcg
     2161 gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca ttgaccaggg
     2221 tattcacaaa atccccacca gatttcagac caacgaccga gaggaacagc acgatcccca
     2281 gctcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg ccgatactgc
     2341 cgatacgccc gaggatcaac gccataatca gcggaccgcc cgccagcccc agtttcaacg
     2401 cggccgggaa tcctggcaca aagacgggaa tagaacctaa caatacgcca agcccgatgc
     2461 caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat
     2521 tggcaacggc atcaattgcg gacggacgcc ccaccaggtt gaggatatcg ccgaactgca
     2581 ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc gagataacaa
     2641 cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg
     2701 tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt agacagcgag gtatcgacct
     2761 cctgaccaat caccagttgc gcattatgta aatccgctgg ctgccccacc agatgcagca
     2821 aatcgcccag ttggataatg gtatctggcg aaggtacttt tagggtttct tctcgtttca
     2881 gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaatg gctaaatcat
     2941 gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg ccattggtgc
     3001 gtgaagactc gtgctgctga gcttctgtct cgacattgac gcggaaaata acccgcaaca
     3061 tccacatggt gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg taactcatcc
     3121 ccatctgatc gaccatttcc attggtgtac ccaggtcgcg caagatctgc tgccctgccc
     3181 ccagcgctgg cgtattggtt accgcaccgg agaaaatccc cagcactacc ggcagtggaa
     3241 tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg atcagaacag
     3301 caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctacct
     3361 gaatcccgat ggtataaacg aacaggatca ggccaaattc ctgaataaca tgcagcatat
     3421 cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca
     3481 gcacgccgcc aatacctaat cctatgccgc gaaatttgac gttgccgata aacaaaccga
     3541 cgactgccac caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct
     3601 cttgagataa cattttaatt gctacggatt atggctgaat cggtgaagca gatttgcctt
     3661 atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc ttaaacagtc gaaatttaac
     3721 gcattgttga agaagaaaga caaaaaaagc cccaccagaa tggcggggca aagagaatag
     3781 ctagttagct atttaacgcg ggacgttcgc tgatagcgat acgctgcgct gcgatgggtt
     3841 caggctcatt acgaattaaa tcaatatgca gtaaaccgtt tacgaaggtt gcgccagaga
     3901 cttccatatt ttcagccagc gtaaaactca ggctaaatgg ctgattcata agcccttgat
     3961 gcagccattt tttctcttct tttggctgct ccggcgtgcc ttttacgctc aggcgcgtac
     4021 cttccagttg aatctctaaa tcttcctgac ggaaacctgc cagcgcaagg gtaatgcggt
     4081 agtggttatc gtcgcttttc tcaatgttgt acggcgggaa gctctggctt tcaccggcgt
     4141 tttgcagtgc gttggccagt ttgtcaaaac cgatccattg acgcatcagt ggggataaat
     4201 cgaagttacg catagtcatt tctccttcta agaagcgagt aagtacctgc aaatccgaag
     4261 attcgcatat gctccctgac ggcgagcatg gagatgtcag gccgcgccag gcggccttag
     4321 ggaattagtt gatttcgata cggcgcggtt ttttcgcttc cggaatcacg cgttcgagat
     4381 cgatatacag caaaccattt accaggttag ccccacgaac atgaatgttc tcagctaact
     4441 ggaatttgcg ttcaaagttg cgttcagcga tgccctggta cagataggtg cgctcttttt
     4501 gttcgtcggc gtgagcacct ttcaccacca gcagattatc ctgggcggta atttccagtt
     4561 cgctctcagc aaaaccagcc acagcgatag caatgcggta atggttttcg tctaccagtt
     4621 caacgttata cggagggtag ccgccattac tctggctctg gttgttttct aagtggttaa
     4681 acaaacggtc aaatccaata gcagaacggt aaagcgggga taaatcaaag ttacgcataa
     4741 tcaatagctc ctgaaatcag cgagaatgta agaccttcca caatggacag gtcaggtagc
     4801 cagaacaccc atcaggcgcg tcctcatcga ctacgatgta aaatgggtct ggaaatgaac
     4861 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     4921 gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg cgacgtaatg atgatgttat
     4981 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     5041 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatgaca ttactcggag
     5101 gatgctccag cgtgatgtcc cataccgggg ggaaagaagg aacgtatccc ggcacccgcg
     5161 ccagcgccac catgattggc gacgatgaaa ccaactgggg cactaagtcg ctggcgattc
     5221 tcgatatgcc gtttaccgct gtgatggaca cgcttttact gccgtgggat gtgttccgca
     5281 aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     5341 cggttattcc accggccaga atgcctgata actgatttag cgatcggttt cggtgatcca
     5401 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     5461 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     5521 ggcacaaaca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     5581 aaatgcagac tgaatatccg ttttgttatg ggttaacttg cgcggttcgc cgccctgtgg
     5641 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     5701 accctgcgga ttacagcgca cccagtggcg cgggtagggt tgtttccgtt ccacttaacg
     5761 gcgcatcacc tgccgatttc cagctagctt catcttgcgg taactcaacg ataaatagct
     5821 ccggcacttt ctcgcccttt ggcgaaagtg tgtcgccaat aaacgccagc gcgtgatttc
     5881 ctacccatcc ttcttcataa gcacgattga tttcatcact gccaggctgc ggcgtgggcg
     5941 tggttttact caccagcaca caccagtggc taccgctgta ttcacgcgga tgttgctttt
     6001 gtacgttgac cgggccaaac ggtgcagcaa ccccgacgtt tcgcaaatcc agcgccggat
     6061 cgagttgatg cattacatgg tcgttatagg taaagctccc cctttcaccg ttcgggctaa
     6121 agacatgcac atggctgcca ccgcgcagcg ctcctggcgt atacggcgcg gtaatatcca
     6181 ttgcatcgag attgctcacc ttgccgcctt cagcaatgac tccgcgtcga tgatggaagt
     6241 cgtaatgcca tgtttcatca ggattttcag ggccgtgaat aaagacatat ttctctgact
     6301 tcggatgaac ggtcaccacg ccgacgtgtg cgccctgtga cgcgcgatag ataacctcga
     6361 cctcgcctgt atggatattc acacgctcaa tggtctcgcc ggtaaacgac gccccagaag
     6421 gacgcacgtc aaataccagc cactggctgt cgggcgtcca ggtattggta ttggtgagca
     6481 ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac aaaacggttc
     6541 ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc atcgtgatga
     6601 atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc ccggcagggt
     6661 cggcgttggc acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat aaaaagcacc
     6721 agtgcggtac tgaaggtttc agcaaacctt gcagattttc agcgtcaaaa ctgtcgacgt
     6781 cgccgcatcg ctcacgcgta actaccagcg aagctatatc aatattaatc gccatgcttt
     6841 cgacttgtgg atgaaatcgc tgatccccgc cagcgttgag gtttatcacg atagcctgtg
     6901 ccgaaaaatc tggcgtgagg atgataaatg gcatgtcatt tttcgtgcag acggctggga
     6961 gcaacatatt accgcccgct atctggtcgg tgccgatggc gcaaactcga tggtgcggcg
     7021 acatctctac ccggatcatc aaatccgtaa atatgtcgct atccagcagt ggttcgcaga
     7081 gaaacatccg gtgccgttct actcctgcat ctttgataat gcgataactg actgttactc
     7141 atggagtatc agcaaagatg gttattttat ctttggcggt gcctatccaa tgaaagacgg
     7201 tcagacgcgt ttcacgacgc tgaaagagaa aatgagcgcc tttcagttcc agtttggcaa
     7261 agcggtgaaa agtgagaaat gcacggtgtt gtttccctca cgctggcagg attttgtctg
     7321 tggtaaggac aacgcctttc tgattggtga agcggcggga tttatcagcg ccagctcgct
     7381 ggaggggatt agctatgcgc tggatagcgc agagattctg cgtgcggtgt tcctgaaaca
     7441 gccggagaag agcaacgccg cttactggcg cgccacacgc aaactgcgtt taaaactctt
     7501 cggcaagata gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt ggattatgcg
     7561 cagtggtgtg gcgcatattc cacagttgaa agattatcca acgcgcttca catcacccac
     7621 cagcaggatg taagagagcg cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg
     7681 cgcgaaaccg taaccctgcg ccaggtaccc caccaccagc ggaacggtga tgccgcccag
     7741 accaccggcg aagttaaaca cgccgccggt taaaccaatc aggcgcatcg gtgccagaga
     7801 agagaccagc gaccaggtaa tcgaagcaaa accgttgccg aagaatgcca gcgccatcag
     7861 gcacataatc atcatcggat cgttggtgta gttagcgccc ataatgcagg tggagatcag
     7921 caagccgcag ataatcggcg ttttacgcgc aaagcccagt gaaaagccct tacgtaccag
     7981 cagatccgcg acccagccag agagcaggac gccgacaaac gccgcgagga atggcaccgt
     8041 ggtcataaag cctgctttca gcgccgtgat tcctttttcc tgggttaaat agttcgggaa
     8101 ccaggttaag aaaaaccaca gtgcagaagc caccgcaaat tgcccaagat aaacgccgat
     8161 cagtttacga tggaacacca gtttccagtc tttggctgtt aacggctgac gcgcctcttt
     8221 cttcaccggc gcatcaccat ccaccagacc gccgccatca cgaatgtaat ccagttcagc
     8281 tttgctgata cctttggtca ggcgcggcgg ctgataaacc ttaaaccaaa tcagcgacca
     8341 gataatgccg ataccaccag tgacaatgaa cacccagtgc cagctcaaca tctcctgaat
     8401 ccagatcagc agcggagtca aaaacgccag accgacaaac tgaccagacg tataaaaacc
     8461 aacggcggaa gcgcgttcat gttccggaaa ccagctggtc accatccggt tattggtcgg
     8521 gaaagcgggc gcttcgaaaa taccggttat cgcacgcaga ccaattaatg acattaatcc
     8581 cgtggcaaag ccctggaata aagtcgccac tgaccaacca aatatcgcaa taaaataagt
     8641 cacgcgagaa cctacgcgat ctaaaaacca accgccgggg atctggcata gcgtataaag
     8701 ccaggcgaag gccgaaaata catagcccat ttccgctttc gtaatgccga actcttcctg
     8761 aatatgggcg gaagccacgg ccaggttagc gcggtcaaca taacaaatga ccaccgtaat
     8821 aaagatcatc accagcgtca gataacgccg acgccccggc tttgctgcat taacgggaat
     8881 atccatagcg agctttctcc agattttggg catagcgaag ccgctcacca tgccctgtaa
     8941 tttacagagg gttatatttt tgtattgctg ttttagtgcc cgatgagggg cttacgtggc
     9001 aggaattacc actctgctac gctgttatct tcatgacgcc agagcggatt acgccagtcc
     9061 ggggcatttt tactgaactc aataacttta gcttcgtcga tttccacacc taagcccggt
     9121 ttcgttaacg gtttaaagaa gccgccaacc atgctgaagt cttctttgtt tttcacaaag
     9181 tcgagtaact ccgcgccttt gttgtaatga atgcccatac tttgttactg aagtaccgcg
     9241 ttataggaaa caaagtcgat atgcaggcaa gccgccagtg caatcggtcc gaacggacag
     9301 tgcggcgcaa gggtcacatc ataggcttct gccattccag caattttgta gcattcggta
     9361 ataccgcctg catgggagag atccggttgc agaatcgaaa taccgcctgc ctccagcacg
     9421 cgtttaaaat cgaaacgtga gaacatgcgc tcacccgccg ccagtggaat atgcgtttgt
     9481 gccgccaatt tcgggtagta ttcggcttgt tccgccagca ccggctcttc aataaacagc
     9541 gggcgatacg gctccagttc tttaatcagc actttcgcca ttggcgcgct gacgcgaccg
     9601 tggaaatcaa gaccaaactc aatctgattg ccaaaagctt cacgaatttg tgccacggtg
     9661 ttgactgccg catctaccgc gcgggagtta tcaattagcc ccagttcttc acaaccgttc
     9721 agtttgaagg tatcgaagcc gatttcgcgc agggttttaa tgccgtcgat aacatccgcc
     9781 ggacgatcgc cgccgaccca actgtaggct ttaattttgt cgcgaaccag gccgcccatc
     9841 agttgccaga ctggcgcatt cagcactttg cctttgatat cccataacgc ctggtcgatc
     9901 ccggcgatgg cgctcatcag gattggaccg ccacgataaa atccggcgcg atacatcact
     9961 tgccataagt cgttgatgcg cgaaggatcc tgaccaatca aatagtcacc cagctcgtga
    10021 actgccgctt ccaccgtacg ggcgcggcct tcaatcacgg gctcgcccca accgaccacg
    10081 ccttcatcgg tttcaatttt caggaacatc cagcggggag gtaaacgata cgtggtaatt
    10141 ttggtgattt tcattgcact gcctctcgat acgccttaac aaatgctgct gcctgctgcg
    10201 cggtgcgttc tacggactgc ccggcgcgat aaagatcgct gcctaagccc gccccaacac
    10261 aacctgcgtc tatccactgc gccaggtttt ctggcgtcac gccgccaacg gcaaagactg
    10321 cgatgtccgg tggtaatacc gcttttaacg ctttgatgta ttgcggacca aaagccgatg
    10381 acggaaatat tttcagcgcc tgcgcgcccg cttcgagcgc ggtaaaggct tcggtcgccg
    10441 tcgcgcagcc ggggcaaacg gtcatgccgt agcccaccgc gcggcggatc acttcactat
    10501 ggatattggg cgtaacgatg agctgacagc ccatcctggc gagcgcatcg acctgttcag
    10561 gtttcagtac cgtacctgcg ccaatcaacg ccttgtcgcc atacgcatca acgatggcgg
    10621 gaatgctctg ctcccattgt ggggaattca gcgggatttc aaccgcgtcg aacccggcgt
    10681 caatcaccgc gccaacatgc gccagcgcct cgtcgggcgt aatgccgcgc aaaatggcga
    10741 tcagcgggag tttagtttgc cactgcatga gcgatgctcc ttataccagc ctgaaatgcc
    10801 gtgtcgcccg ccaccgccgc cacgtcgcaa cccatcgcct gaaaggcttg ctggtagcgc
    10861 gcggtcagcg atgttccggc gacaagggtg atggcgtgtt gatgggtcac atagtcgcgc
    10921 atactggcaa cctctgcgcc aatcaacaaa ccagagagaa tgttcgcgga gaagtgttcc
    10981 cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg cattaaggcc
    11041 gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca aacctgcgcc
    11101 aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc
    11161 gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca taacataaag
    11221 agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc gcatcacatt
    11281 gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga
    11341 cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt
    11401 ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt cttcacgcca
    11461 gtcggtcgtg acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc
    11521 tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt
    11581 ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt
    11641 gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt
    11701 atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc
    11761 atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt
    11821 aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta
    11881 aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc
    11941 agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt
    12001 cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc
    12061 agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc cccgcgataa
    12121 cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg gatgatgttg
    12181 cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga gccaggtacg
    12241 tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt tttattgaga
    12301 gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa
    12361 cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgtgcgt gtaatgtagt
    12421 acaaacttat attgttgtgc tacaatttag atcaaaaaaa gaacaatgca taaaaaaatg
    12481 acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga
    12541 gtatgctttc aacggcttag ctactcgttt aaaagattaa tcatgaagtt gaattttaag
    12601 ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg ctgtatctcg
    12661 tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac
    12721 accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta tgtttttgta
    12781 ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa gatgctcaat
    12841 gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt tgttttaaat
    12901 gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa taacgaagaa
    12961 gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg taccaggtca
    13021 ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta ctcaaaggtg
    13081 atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc cagaggcatt
    13141 ccggatggtg tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca
    13201 ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt
    13261 ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac
    13321 gccatcttca aggttggatt ttgtgacaaa gttcgccatt tctttcaccg aaggaatagc
    13381 gttacccatc gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc
    13441 gccaatcgcc atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc
    13501 cgtacctttg ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac
    13561 ggtatatttc tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg
    13621 ttcatcaatc atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca
    13681 gaacaccagc ggaattgtgg caacgaagga ttcatgcacc gtgtagtagc tgatatcgcg
    13741 gttagcggtg tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga
    13801 gagtttttcc aggaaacgat agtcgtcata gctgagagcg gtttgcgcca cggtgctacc
    13861 atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc
    13921 catatgcagc tctttcaggt agttgtgcac acctgcatac ggacgacccg tcgttagcac
    13981 gacattcacg ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt
    14041 gtgatcgggc agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag
    14101 ttcccaaggt agattagttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca
    14161 acacccgagg gaaaagggga tgcaacgcgg gttgcatgct ccccatttgc ttagcggaag
    14221 agtttccctt tcagcagttc catgcctgcg gaaagcagat cgttattggc ttgtgctgac
    14281 acttcacctt gcggtgagag cgcatcaata atcttcggca attgctctgc cagtaaactg
    14341 gaagccgtac tggtatccac gccaagtttt tgcccgagat cggacaccgc atttgtgccg
    14401 agcgccgatt ccagttgctc gccgctaact gattgatttc tctgttgatt actcaaccaa
    14461 gttgagagaa tggcccctaa gccgccactt tgcagttttt ccagcagcac ctgaatgcca
    14521 ccctgctcct caacccaact taaaatcgcc tgatatttcc ccgcatcgcc tttcagaaag
    14581 gcaccgacaa cttcatcaaa aagccccatg ataatcacct gtaaagcgtt acgtgttgac
    14641 ccaaaaagta tagatctgcg ggatgataat tgcggattgc agaaataaaa agggcggaga
    14701 tgatctccgc ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc ttatccggcc
    14761 tacaaaatca tgcaaaatca atatattgca ggagtctcgt aggactaata agcgtagcgc
    14821 atcaggcacg ctcgcatggt taacgccatt aaatatcaat attcgccgct ttcagggcgt
    14881 tctcttcaat aaacgcacga cgcggttcta cggcgtcgcc catcagcgtg gtgaacaact
    14941 ggtcagcggc aatcgcatcc ttaacggtaa cgcgcagcat acgacggctt tccgggtcca
    15001 tggtggtttc ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg
    15061 agaggccacg acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg
    15121 gctgacgacg ctcgccacgt tcgataaacg catcttcttc cagcaagcca cgcagtttct
    15181 cacccagcgt gcagatacga cgatattcgc caccggtgat aaactcgtga tccagcggat
    15241 agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg
    15301 cattggtgtg gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc
    15361 tgaccagcgc gttcacccag cgggtaacgg tctgctcatc agaaaggtca gcttccgtca
    15421 acgtcggctg atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt
    15481 tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt ctctaacgct tcgccagcca
    15541 atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata gagatctggt
    15601 actgatccat cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt
    15661 acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat
    15721 agaagaaggt caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga
    15781 tgatgatgct gtgataacgc agtttgtccg ggttgtactc gtcacgaccg ataccacagc
    15841 caagcgcggt gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt
    15901 tctcgacgtt aaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc
    15961 cctgcttcgc agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg
    16021 gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc agctaagtcg agcgcacctt
    16081 tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa
    16141 ttttgccaac cacgattttc gcatcagttg ggttttccag caggtattcc gccagcagtt
    16201 cgttcatctg ctgttcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct
    16261 gggaggagaa tttcgggtcc ggcactttta cggaaacgac cgcaatcagg ccttcacgcg
    16321 catcgtcacc ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg
    16381 cattcagggt acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct
    16441 gcggaatgtt gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcagcg
    16501 ccacttcaac gccaataccg tctttttcgg tggagaagta gaagatattt gggtggatcg
    16561 gcgttttgtt cttgttcaga tattcaacga acgctttgat gccgccttca tagtggaagt
    16621 ggtcttcttt gccgtcacgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg
    16681 acaactcacg cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg
    16741 tttcgaggct gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg
    16801 ccagcggagc ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct
    16861 gaataaccag ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca
    16921 gaccgccgga cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca
    16981 tgatcacttc cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc
    17041 cgtcatcctg tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt
    17101 gacccgcgag cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac
    17161 cggtgccgtc atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc
    17221 ctttcaggac tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat
    17281 ttatacttgg gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg
    17341 tccgacatgt ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc
    17401 gcttttaagc ggctggcaag caacccgcga cgctcatcat caagctcaga ggcaaaatca
    17461 tctatcaggt agagacaccg ccgcccgctt tcacgagtga ggaactctcc ttgcgccaga
    17521 cgtaaggcgc acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca
    17581 ccgtcggcgc gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta ggttagctgg
    17641 cgatcgcgtt caaaattacg ttccagcacc tcagcgtact ctgtctcttt ctcccagccg
    17701 cgctggaaag agaaagtcag agaaaactca gggagaaatt gcttacaggt atcatccata
    17761 tcggccgcga taccggcgct atactccgcg cgccaggtgc tgatttgctc cgccagcggg
    17821 atcagttctt tatcccacgg gcgtagctgt tcgtaacgag tcacctggcg cagcgccgca
    17881 ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg tgaaaaatcc aggttcgttg
    17941 tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta
    18001 aacccttctg gcgttatcaa ctgcatcggc atcaggtgcg ccagttccgc gactttatgc
    18061 ccgtccgtac cgtcaatacg gactttgctg tcgccctgtt tgtctttggt taagccaatc
    18121 gccgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa caaatgcctc ctgctcatgg
    18181 cgaatgacgc gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg
    18241 gcttccagca cgctggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga
    18301 gataaagcga gatccgcggt ttcaatattg cggaaatcgc ggatcaacaa gcgggtgagg
    18361 gacattacag tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa
    18421 tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagcgcgt
    18481 tcagaacatc cagcacatag ctgacgttga agccgatttc catctccgca ccgctatagg
    18541 taacgtcgag gatctcttcc gcttcttcct gttccgggtt gttggcagta attttcagct
    18601 ggttttcgct gacatacagg cgcacaccgc ggaatttctc gttagagaga atcgccgcgc
    18661 gagcaaacgc ctgcttgagc agatcgcagc ctgcttccag atgtttgtcc gggttcttcg
    18721 gcagaacgcg acggtaatcc gggaagcgac catccaccag tttcgaggtg aaaataaagt
    18781 cgccaacgtg ggcacgaatg ttattgctgc caatctgcac acgcagcaga ttgtcgccgc
    18841 cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg tacgatcacc gaatggcttg
    18901 gcaaagattg accaattggc attgaacaga ccgccagacg gtggccgtcg gttgccacgg
    18961 tgcgcagttc ttcaccttcg gtttcaaaca gcataccgtt taagtaatag cgaacatcct
    19021 gatgcgccat cgaaaactgg gttgcttcaa tcagacgctt catcgtcgcc tgcggcaggg
    19081 taaattcgac ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag
    19141 acagcgaaaa acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgcacgg
    19201 caatttccgc gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg
    19261 tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacgcg tgccaccatt tccatctcga
    19321 gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa
    19381 tcggtagcgt aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat
    19441 gctcacgttc tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa
    19501 aaatcttctt tgatatcgtg gctctcttca cgcaactgct cgatcttacg gcaggcatga
    19561 agcaccgtcg tgtggtcacg cccaccaaac gcatcgccaa tctctggcag actgtggtta
    19621 gtcagctctt tcgctagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga
    19681 cgcttggaaa ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata
    19741 ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg
    19801 aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag caataacgcg gttcagcgcc
    19861 ccttccagct cacgtacgtt agatcgtaga cgcttggcga taaagaacgc cacttcaccc
    19921 ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga tcgccacacg ggtttccagc
    19981 tctggcggtt cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca
    20041 acgccgttga tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc
    20101 agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac
    20161 tgaatatcgt cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc
    20221 gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc
    20281 actttggcat tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt
    20341 ttacccagac ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc agggttatcc
    20401 gccacctggc gagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca
    20461 aacgtgtgtt tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta
    20521 tcccaacctg agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca
    20581 ggggccgcga cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg
    20641 acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata
    20701 ttattaaggt acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggac
    20761 agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct
    20821 ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac
    20881 tccactcgaa caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg
    20941 tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc
    21001 aagatcctgc aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct
    21061 gtttctcaca gattttccct atttatccac aggactttcc agaactcgct aagtgtaaac
    21121 gatcctgccg cgaggcgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga
    21181 caaaaattgg cttaatcgat ctaataaaga tccaggacga tccttgcgct ttacccatca
    21241 gcccgtataa tcctccaccc ggcgcgccat gctggtttcc actggtgtga ggtcgtacat
    21301 tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggtg
    21361 tgtacaatta ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca
    21421 tttttcggcg gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca
    21481 tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc
    21541 gtatggctac taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc
    21601 gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg
    21661 gagttacgct tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct
    21721 ggcacgccgc aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt
    21781 cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgtctg
    21841 acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg
    21901 gcgaaaaaag gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta
    21961 tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac
    22021 gcctgattag tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg
    22081 gaattgaggc attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg
    22141 tattaaaatg ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg
    22201 ataccagaga acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt
    22261 cgtgtctttc atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca
    22321 acagaccacg cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc
    22381 cagtggccag gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac
    22441 ccgtggtggt gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac
    22501 ccagccgttc cagctgttgg aaacttcacc gcagtttatt tatcaggcac agagcggtct
    22561 gaccggtcgt gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga
    22621 aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac
    22681 cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt
    22741 caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct cctcgtttgg
    22801 tcagttgaag caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc
    22861 actgcacacc ttccgtggcg cggcgtactc cacgcctgac gagaagtatg agaaatacaa
    22921 gttcgatacc attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc
    22981 gatgctgcaa cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt
    23041 ctataccgct aatctgggta acggcatcgt cgctatcggc tataaatctc agccggtact
    23101 ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca
    23161 ggacaaaatg gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg
    23221 gttcatctct cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg
    23281 gggcttctcc attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa
    23341 agcgcagtac acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg
    23401 tgagcgtctg ggcgatgaca aacagcgtat cagtcaggaa atgatggcgc tgtacaaagc
    23461 tgagaaggtt aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct
    23521 ggcgttgtac tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg
    23581 gatccacgac ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac
    23641 gatgttcttc attcagaaga tgtcgccgac cactgtgacc gacccgatgc agcagaagat
    23701 catgaccttt atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt
    23761 actgtactat atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg
    23821 tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc
    23881 gctaaaataa gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg
    23941 cgcatccgcc acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca
    24001 ggccacacct ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg
    24061 tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc
    24121 gtttaaagac gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc
    24181 gaactcgttc accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct
    24241 cgacctgctg ttaaaacgca ttctgaccat tcccggcctg cggattgctc gccctggtga
    24301 gttttccgaa cgcgcgtttc ttaacgataa acttgactta gcccaggccg aggcgattgc
    24361 cgatcttatc gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg
    24421 cgcattctcc gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt
    24481 cgaagcggca attgatttcc ctgatgaaga gatcgatttc ctctccgacg gcaaaattga
    24541 agcccagctt aatgacgtga tggccgatct cgatgcagtg cgtgctgaag cacgtcaggg
    24601 tagtttgttg cgcgaaggga tgaaagtagt gattgccgga cgtcctaatg ccggtaaatc
    24661 gagcctgtta aacgcgctgg cgggccgtga agcggcaatc gtaaccgata tcgccggaac
    24721 cacgcgtgac gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga
    24781 cactgccggg ctacgtgagg ccagtgacga agtggaacgt attggtatcg agcgcgcgtg
    24841 gcaggaaatt gaacaggccg accgcgtgct gtttatggtc gatggcacca cgacagacgc
    24901 cgttgatccg gcagagatct ggccggaatt tatcgcccgt ctgcccgcga aactgccgat
    24961 caccgtggta cgcaataaag ccgatatcac cggcgaaacg ctgggaatga gtgaagtgaa
    25021 cggtcacgcg ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg tgctgcgtaa
    25081 ccatctcaaa cagagcatgg gctttgacac caatatggaa ggcggcttcc tggcgcgccg
    25141 tcgccactta caggcgctgg aacaggcagc ggagcatttg cagcaaggta aagcgcaact
    25201 gttgggagcc tgggcaggtg aactgctggc ggaagagttg cgcctggcac agcagaactt
    25261 aagcgaaatc accggggaat ttacttcaga cgacctgctg gggcggattt tctccagctt
    25321 ctgtattggt aagtaaccgc gcttacgaag ccgtattctg actgtcagat gcggcttcgc
    25381 ttcattgtta gcactcctgt tatgcctcaa ccctttttta aacattaaaa ttcttacgta
    25441 atttataatc tttaaaaaaa gcatttaata ctgctccccg aacgattgtg attcgattca
    25501 catttaaaca atttcagaat agacaaaaat tctgagtgta ataatgtagc ggcgtgtctt
    25561 tcgaggataa gtgcattatg aatatcttac atgtatgtgt gacctcaaaa tggttcaata
    25621 tcgacaataa gattgtcgat caccgccctt aatttaccct tctgtagcca tcaccagagc
    25681 caaaccgatt agattcaatg tgatcgatta gtttgctata tcttaatttt gccttttgca
    25741 aaggccatct ctcgtttatt tacttatttt agtaaatgat ggtgcttgta tatatacatg
    25801 gtgaattaat ggatattgca gatgtaatat tcacagggat cactgtaatt aaaataaatg
    25861 aaggattatg taatggaaaa ctttaaacat ttccctgaac cgttccgcat tcgtgttatt
    25921 gagccagtaa aacgtactac tcgcgcttat cgtgaagaag caattattaa atccggtatg
    25981 aacccgttcc tgctggatag cgaagatgtg tttatcgatt tactgaccga cagcggcacc
    26041 ggggcggtaa cccaaagtat gcaggcagcg atgatgcgcg gcgacgaagc ctacagcggc
    26101 agccgcagct actatgcgtt agccgagtca gtgaaaaata tctttggtta tcaatatact
    26161 attccgactc accagggccg tggcgcagag caaatctata ttccggtact gattaaaaaa
    26221 cgcgagcagg aaaaaggcct ggatcgcagc aaaatggtgg cattctctaa ctatttcttt
    26281 gataccacgc agggccatag ccagattaac ggctgtaccg tgcgtaacgt ctatatcaaa
    26341 gaagccttcg atacgggcgt gcgttacgac tttaaaggca actttgacct cgaaggatta
    26401 gaacgcggta ttgaagaagt tggcccgaat aacgtgccgt atatcgttgc aaccatcacc
    26461 agtaactctg caggtggtca gccggtttca ctggcaaact taaaagcgat gtacagcatc
    26521 gcgaagaaat acgatattcc ggtggtaatg gactccgcac gctttgctga aaacgcctat
    26581 ttcatcaagc agcgtgaagc agaatacaaa gactggacca tcgagcagat cacccgcgaa
    26641 acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat ggtgccgatg
    26701 ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg tgtacaccga gtgcagaacc
    26761 ctttgcgtgg tgcaggaagg cttcccgaca tatggcggcc tggaaggcgg cgcgatggag
    26821 cgtctggcgg taggtctgta tgacggcatg aatctcgact ggctggctta tcgtatcgcg
    26881 caggtacagt atctggtcga tggtctggaa gagattggcg ttgtctgcca gcaggcgggc
    26941 ggtcacgcgg cattcgttga tgccggtaaa ctgctgccgc atatcccggc agaccagttc
    27001 ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt
    27061 ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg cccggctgaa
    27121 ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga cttcattatt
    27181 gaagccttta aacatgtaaa agagaacgcg gcgaatatta aaggattaac ctttacctac
    27241 gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt aatactacag
    27301 agtggctata aggatgttag ccactctctt accctacatc ctcaataaca aaaatagcct
    27361 tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc attttggggt
    27421 gttatggtta tagcaggtac agtaattggt ggaggtatgt ttgctttacc tgttgatctt
    27481 gccggtgcct ggtttttctg gagtgccttt atccttatca ttgcctggtt ttcaatgctt
    27541 cattccgggt tattgttatt agaagcaaat ttaaattatc ctgtcggctc cagttttaac
    27601 accatcacca aagatttaat aggtaacacc tggaacatta tcagcggtat taccgttgcc
    27661 ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
    27721 acgatatcaa tgaatttggg ctatcacgct aatccacgta ttgtcgggat ctgcacagcc
    27781 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgcat tacctcattg
    27841 ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt cttccaggtc
    27901 gattactcca tcctgcgcga tgccaccagc accactgcgg gaacgtctta cttcccgtat
    27961 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
    28021 ctgattattt gctatggcaa acgcaaagat aagttaatca aaagcgtggt atttggttcg
    28081 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tattccgcgc
    28141 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
    28201 ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc taacttagct
    28261 gttgccagtt cgttctttgg tgtcacgctg gggttgttcg attatctggc ggacctgttt
    28321 aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
    28381 gcgttgttgt atctgatctt cccgaacggc tttatttacg ggatcggcgg tgccaggctg
    28441 tgcgccacca tctgggcggt cattattccc gcagtgcttg caatcaaagc tcgcaagaag
    28501 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
    28561 tttggtataa ccgtgatttt gtgctggttc ggcaacgtct ttaacgtgtt acctaaattt
    28621 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
    28681 tgtccaaatg gcaactcgcc ttatcctcct tcaccacgta tgctttgcgt caccttacta
    28741 tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg gttttacttt
    28801 atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
    28861 ccagcgaagc gcagttgcat attgcgttct ccgtatatct ggcggggatg gcagctgcga
    28921 tgctatttgc cggtaaagtg gccgatcgtt cagggagaaa gccggtcgcc atacccggcg
    28981 cggcgctatt tattattgcc tcggtgttct gttcactggc tgaaaccagc acgttatttc
    29041 ttgcaggccg atttctacag gggttgggcg caggctgttg ttacgtggtg gcgttcgcca
    29101 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcatta ctcaacggta
    29161 ttacctgcat cattccggtg ttagcgccag tgctcggaca tctgattatg cttaaattcc
    29221 cgtggcagag tctgttctgg gcgatggcaa tgatgggcat cgcggtactg atgttgtctt
    29281 tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa
    29341 atagcgagtc gctgcttaat cgttttttcc tcagccgtgt tgttatcacc accctcagcg
    29401 tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa atcatggggt
    29461 ttgagcgcgg agaatacgcc accattatgg cgctgaccgc tggcgtcagc atgacctttt
    29521 cattctcgac gccatttgcg ctgggaattt ttaagccacg tacgttgatg atcacctcgc
    29581 aggtgttatt ccttgcggcg gggatcaccc ttgccgtttc accttcccat gcggtttctc
    29641 tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc
    29701 aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
    29761 aggtttgcgg ttcgtcactg tggatttggc tggccgcggt ggttggtatc ggcgcatgga
    29821 atatgctgat cgggattctg attgcctgta gcattgtgag cctgttgctg attatgttcg
    29881 tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
    29941 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
    30001 atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
    30061 gacccgctct ttgtgaactc accgctgggg ctgtcgccca cacagctaat ggtcagcatg
    30121 gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa accgcaccac
    30181 cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat gatgatcatg
    30241 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaactacgt
    30301 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
    30361 ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt agccattgat
    30421 tatgaagtgc tgtttagtga tgtaccctgc gtctggttac gcaaagatca tccggcactg
    30481 catgaaacgt ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
    30541 cagagcgaca cct
//
LOCUS       DADLZM010000035        30014 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLZM010000035 DADLZM010000000
VERSION     DADLZM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.035"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            250..633
                     /gene="secE"
                     /locus_tag="JNY73_001563"
     CDS             250..633
                     /gene="secE"
                     /locus_tag="JNY73_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY8047614.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            635..1180
                     /gene="nusG"
                     /locus_tag="JNY73_001564"
     CDS             635..1180
                     /gene="nusG"
                     /locus_tag="JNY73_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY8047615.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1339..1767
                     /gene="rplK"
                     /locus_tag="JNY73_001565"
     CDS             1339..1767
                     /gene="rplK"
                     /locus_tag="JNY73_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY8047616.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1771..2475
                     /gene="rplA"
                     /locus_tag="JNY73_001566"
     CDS             1771..2475
                     /gene="rplA"
                     /locus_tag="JNY73_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY8047617.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2888..3385
                     /gene="rplJ"
                     /locus_tag="JNY73_001567"
     CDS             2888..3385
                     /gene="rplJ"
                     /locus_tag="JNY73_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY8047618.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3452..3817
                     /gene="rplL"
                     /locus_tag="JNY73_001568"
     CDS             3452..3817
                     /gene="rplL"
                     /locus_tag="JNY73_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY8047619.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4137..8165
                     /gene="rpoB"
                     /locus_tag="JNY73_001569"
     CDS             4137..8165
                     /gene="rpoB"
                     /locus_tag="JNY73_001569"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY8047620.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8242..12465
                     /gene="rpoC"
                     /locus_tag="JNY73_001570"
     CDS             8242..12465
                     /gene="rpoC"
                     /locus_tag="JNY73_001570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY8047621.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12707..13840)
                     /gene="thiH"
                     /locus_tag="JNY73_001571"
     CDS             complement(12707..13840)
                     /gene="thiH"
                     /locus_tag="JNY73_001571"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY8047622.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13837..14682)
                     /gene="thiG"
                     /locus_tag="JNY73_001572"
     CDS             complement(13837..14682)
                     /gene="thiG"
                     /locus_tag="JNY73_001572"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY8047623.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIVDKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14609..14809)
                     /gene="thiS"
                     /locus_tag="JNY73_001573"
     CDS             complement(14609..14809)
                     /gene="thiS"
                     /locus_tag="JNY73_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY8047624.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14793..15548)
                     /gene="thiF"
                     /locus_tag="JNY73_001574"
     CDS             complement(14793..15548)
                     /gene="thiF"
                     /locus_tag="JNY73_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY8047625.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15541..16176)
                     /gene="thiE"
                     /locus_tag="JNY73_001575"
     CDS             complement(15541..16176)
                     /gene="thiE"
                     /locus_tag="JNY73_001575"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY8047626.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16176..18071)
                     /gene="thiC"
                     /locus_tag="JNY73_001576"
     CDS             complement(16176..18071)
                     /gene="thiC"
                     /locus_tag="JNY73_001576"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY8047627.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18152..18252)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18305..18781)
                     /gene="rsd"
                     /locus_tag="JNY73_001577"
     CDS             complement(18305..18781)
                     /gene="rsd"
                     /locus_tag="JNY73_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY8047628.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18876..19649
                     /gene="nudC"
                     /locus_tag="JNY73_001578"
     CDS             18876..19649
                     /gene="nudC"
                     /locus_tag="JNY73_001578"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY8047629.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19689..20753
                     /gene="hemE"
                     /locus_tag="JNY73_001579"
     CDS             19689..20753
                     /gene="hemE"
                     /locus_tag="JNY73_001579"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY8047630.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20910..21581
                     /gene="nfi"
                     /locus_tag="JNY73_001580"
     CDS             20910..21581
                     /gene="nfi"
                     /locus_tag="JNY73_001580"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY8047631.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21624..22214
                     /locus_tag="JNY73_001581"
     CDS             21624..22214
                     /locus_tag="JNY73_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY8047632.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22401..22673
                     /gene="hupA"
                     /locus_tag="JNY73_001582"
     CDS             22401..22673
                     /gene="hupA"
                     /locus_tag="JNY73_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY8047633.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22686..23381
                     /locus_tag="JNY73_001583"
     CDS             22686..23381
                     /locus_tag="JNY73_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY8047634.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23383..23808)
                     /gene="zraP"
                     /locus_tag="JNY73_001584"
     CDS             complement(23383..23808)
                     /gene="zraP"
                     /locus_tag="JNY73_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY8047635.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24046..25422
                     /gene="zraS"
                     /locus_tag="JNY73_001585"
     CDS             24046..25422
                     /gene="zraS"
                     /locus_tag="JNY73_001585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY8047636.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25419..26744
                     /gene="zraR"
                     /locus_tag="JNY73_001586"
     CDS             25419..26744
                     /gene="zraR"
                     /locus_tag="JNY73_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY8047637.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26741..28030)
                     /gene="purD"
                     /locus_tag="JNY73_001587"
     CDS             complement(26741..28030)
                     /gene="purD"
                     /locus_tag="JNY73_001587"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY8047638.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28042..29631)
                     /gene="purH"
                     /locus_tag="JNY73_001588"
     CDS             complement(28042..29631)
                     /gene="purH"
                     /locus_tag="JNY73_001588"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY8047639.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7029 a         7826 c         8254 g         6905 t
ORIGIN      
        1 tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact aaaagggcat
       61 catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt gattttcttt
      121 gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa gctaatacgc
      181 gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa acttctgaca
      241 ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct ggaagcgatg
      301 aagtgggtcg ttgtggtggc attgctcctg gcggcgattg tcggcaacta cctttatcgc
      361 gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc agcgggtggt
      421 gtcgcgctgt taacgacaaa aggtaaagct accgttgctt ttgcccgtga agcgcgtacc
      481 gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac gctgattgtg
      541 gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat tctggttcgc
      601 ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta aaaagcgctg
      661 gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc tgcgtgagca
      721 tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa ccgaagaagt
      781 ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc ctggctacgt
      841 cctcgttcag atggtgatga acgacgcgag ctggcacttg gtgcgcagcg taccgcgtgt
      901 gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata aagaagtcga
      961 tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa cgctgtttga
     1021 accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg gtgttgttga
     1081 agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg gtcgtgcgac
     1141 cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc aaaaaagcgg
     1201 cgatttaatc gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt ttgtttttat
     1261 gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg cgttattacc
     1321 caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct gcaggttgca
     1381 gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca gggcgtaaac
     1441 atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa aggtctgccg
     1501 attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac caagaccccg
     1561 ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg taagccgaac
     1621 aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca gaccaaagct
     1681 gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg tactgcacgt
     1741 tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg catgcgtgtt
     1801 atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat cgcactgctg
     1861 aaagagctgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa cctcggcatc
     1921 gatgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca cggtactggc
     1981 cgttccgttc gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc taaagctgca
     2041 ggcgcagaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg cgaaatgaac
     2101 tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct gggccaggtt
     2161 ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacacc gaacgttgct
     2221 gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa aaacggcatc
     2281 atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga aaacctggaa
     2341 gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt gtacatcaag
     2401 aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc tggcctgagc
     2461 gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct tacccccacg
     2521 tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg cggcaactga
     2581 gtgagtcgcc aggagcatag ctaactatgt gactggtgcg aatgaaggaa gccaacgccg
     2641 ccacaagttg aatagcgacg gatagaaaag atttgttcgt tggagcctgg cctatccagg
     2701 cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta gacggtgaca
     2761 gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa ttaagacgct
     2821 ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac atccaggagc
     2881 aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag tcagcgaagt
     2941 agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg tagataaaat
     3001 gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg ttcgtaacac
     3061 cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg cgtttgttgg
     3121 tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc tgttcaaaga
     3181 gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag gtgagctgat
     3241 cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag caattgcacg
     3301 cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc tggctgctgt
     3361 acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc gcttacgtat
     3421 aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc aaatcattga
     3481 agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa tggaagaaaa
     3541 attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg aagctgctga
     3601 agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag ttgctgttat
     3661 caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc tggtagaatc
     3721 tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac tgaaaaaagc
     3781 tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg gttgcagcct
     3841 gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg ctgtaaggcg
     3901 ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt ttctatcgac
     3961 gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa tggtttaagc
     4021 gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa tagtgttgca
     4081 caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg aaccctatgg
     4141 tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt ccacaagttc
     4201 tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt atcgagcaag
     4261 atcctgaagg gcagtatggt ctggaagctg ctttccgttc tgtattcccg attcagagct
     4321 acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg gtgtttgacg
     4381 tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt aaactgcgtc
     4441 tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa gaacaagaag
     4501 tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc aacggtactg
     4561 agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac tccgacaaag
     4621 gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct taccgtggtt
     4681 cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc gaccgtcgcc
     4741 gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag cagatcctcg
     4801 acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag atggaactgg
     4861 tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac ggtaaagtgt
     4921 acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa aaagacgacg
     4981 tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct aaagactata
     5041 ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc ctggatctgc
     5101 tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc aacgatctgg
     5161 atcacggccc atatatctct gaaaccttac gtgtcgaccc aactaacgac cgtctgagcg
     5221 cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt gaagcagctg
     5281 aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacttgtct gcggttggtc
     5341 gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt attctgagca
     5401 aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt aaaggcgaag
     5461 tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa atggcggaaa
     5521 accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt ctgtctctgg
     5581 gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt tccgcagcag
     5641 tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac aacccgctgt
     5701 ctgagattac gcacaaacgt cgtatctccg cactcggccc aggcggtctg acccgtgaac
     5761 gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta tgtccaatcg
     5821 aaacccctga aggtccgaac atcggtctga tcaactctct gtccgtgtac gcacagacta
     5881 acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt gtaactgacg
     5941 aaattcacta cctgtctgct atcgaagaag gcaactacgt tatcgcccag gcgaactcca
     6001 acctggatga aggaggccac ttcgtagaag acctggtaac ttgccgtagc aaaggcgaat
     6061 ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag caggtggtat
     6121 ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt gcattgatgg
     6181 gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgataagccg ctggttggta
     6241 ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct aaacgtggtg
     6301 gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa gacgagatgt
     6361 atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt tctaaccaga
     6421 acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa cgtggcgacg
     6481 tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag aacatgcgcg
     6541 tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta tccgagcgtg
     6601 ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt gtgtcccgtg
     6661 acaccaagct gggtccggaa gagatcaccg ctgacatccc gaacgtgggt gaagctgcgc
     6721 tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc ggtggcgaca
     6781 ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa gaaaaactgc
     6841 tgcgtgcgat cttcggtgag aaagcctctg acgttaaaga ctcttctctg cgcgtaccaa
     6901 acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc gtagaaaaag
     6961 acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa gacctgtctg
     7021 aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg ctggtagccg
     7081 gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg gagctgggcc
     7141 tgacagacga agagaaacaa aatcagctgg aacagctggc tgagcagtat gacgaactga
     7201 aacacgagtt cgagaagaaa ctcgaagcga aacgccgaaa aatcacccag ggcgacgatc
     7261 tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc cgtatccagc
     7321 ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag atcaacccga
     7381 tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg aacccgctgg
     7441 gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt atggctgcga
     7501 aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc gcgaaactgc
     7561 gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa gttgacctga
     7621 gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa ggtatgccaa
     7681 tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg ctgaaacttg
     7741 gcgacctgcc gacttccggt cagatccgcc tgtacgatgg tcgcactggt gaacagttcg
     7801 agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg gtcgacgaca
     7861 agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg ctgggtggta
     7921 aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg gaagcatacg
     7981 gcgcagcata caccctgcag gaaatgctca ctgttaagtc tgatgacgtg aacggtcgta
     8041 ccaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc atgccagaat
     8101 ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa ctggaagacg
     8161 agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc ggattgtgct
     8221 aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc gcagactaaa
     8281 accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat ccgttcatgg
     8341 tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa accagaacgt
     8401 gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg cctgtgcggt
     8461 aagtacaagc gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt tgaagtgacc
     8521 cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc gactgcgcac
     8581 atctggttcc tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat gccgctgcgc
     8641 gatatcgaac gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg tatgaccaac
     8701 ctggaacgtc agcagatcct gactgaagag cagtatctgg acgcgctgga agagttcggt
     8761 gacgaattcg acgcgaagat gggtgcggaa gcaatccagg ctctgctgaa gagcatggat
     8821 ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc cgaaactaag
     8881 cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc tggtaacaaa
     8941 ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg tccgctggtt
     9001 ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg tcgcgtcatt
     9061 aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat catcgtacgt
     9121 aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg tcgtcgcggt
     9181 cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat gatcaaaggt
     9241 aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc cggtcgttct
     9301 gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa gaaaatggcg
     9361 ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct tgctaccacc
     9421 attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga tatcctggac
     9481 gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca ccgtctgggt
     9541 atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca cccgctggtt
     9601 tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt accgctgacg
     9661 ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat cctgtccccg
     9721 gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct gtactacatg
     9781 acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc gaaagaagca
     9841 gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt gcgtatcacc
     9901 gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa agacacgact
     9961 gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc catcgtcaac
    10021 caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg cattctcggt
    10081 ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc ctatgcagcg
    10141 cgttctggtg catctgttgg catcgatgac atggtcatcc cggagaagaa acacgaaatc
    10201 atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc tggtctggta
    10261 actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa cgatcgtgta
    10321 tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga cggtcaggaa
    10381 gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc gcgtggttct
    10441 gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcaaagcc ggatggctcc
    10501 atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact ccagtacttc
    10561 atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac tgcgaactcc
    10621 ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac cgaagacgat
    10681 tgtggtaccc atgaaggtat catgatgact ccggttatcg agggtggtga cgttaaagag
    10741 ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa gccgggtact
    10801 gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga cctgctggaa
    10861 gagaactctg tcgacgcggt aaaagtacgt tctgttgtat cttgtgacac cgactttggt
    10921 gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa caaaggtgaa
    10981 gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct gaccatgcgt
    11041 acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat ccaggtgaaa
    11101 aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag cggtaaactg
    11161 gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg taccaaagaa
    11221 agctacaaag tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca ggttgctggc
    11281 ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga agtaagcggt
    11341 tttgtacgct ttactgacat gatcgacggc cagaccatta ctcgtcagac cgacgaactg
    11401 accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg tggtaaagat
    11461 ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat cccaggtacc
    11521 gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga agatggcgta
    11581 cagatcagct ctggtgacac cttggcgcgt attccgcagg aatccggcgg taccaaggac
    11641 atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag ctcgtcgtcc gaaagaaccg
    11701 gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa aggtaaacgt
    11761 cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat tccgaaatgg
    11821 cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat ttccgacggt
    11881 ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac tcgttacatc
    11941 gttaacgaag tacaggacgt ataccgtctg cagggcgtaa agattaacga taaacacatc
    12001 gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg cagctccgac
    12061 ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg cgaactggaa
    12121 gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac caaagcgtct
    12181 ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg cgtgctgacc
    12241 gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa cgttatcgtg
    12301 ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat gcgtcgccgt
    12361 gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc tgccagcctg
    12421 gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt aatccgcaaa
    12481 taacgtaaaa acccgcttcg gcgggttttt ttatgggtgt tattcagagg gcaaattctt
    12541 cacggaaaat attaagaaag gcagggcgaa acgaggaaga agcttttcgc cctggacaac
    12601 attcctgctg acggcactac cataaaaaaa cacaacgctt tcaacaacct tgcgtttttc
    12661 atcttccgta gcggctcgga ataaatttac gttcttacat tccgtctcat agtctttgcg
    12721 aggcgcgtcc cagatagctg tcccagtctt tccataccgg ctgcaaaccc tgagcggtta
    12781 acgcggcagc aaccgcttcc ggtctgcgat cgtcgtgcgg tgagaactgt tccaactcgg
    12841 ggtgattatc ggcatagcca cctggctgcg ttttcgagaa ggcgctgacg ttattgatcg
    12901 ccagcggaat aacgcgatcg cgaaaccacg gtgattcccg cgtggagagt gacagttcaa
    12961 tctccggtgc aagcagtcgg aaggcgcaga tggtttgcac taactggcgt tcatccataa
    13021 tcgacgcagg ctcaatgccg ccagtacacg ggcgcaggcg cggaaaggag acagagtaac
    13081 ggctttgcca gtaatgctgt tgcagccata gcaaatgttc ggcaaccata tagcagtcaa
    13141 cgcgccagtt gtcggaaagg ccaattagcg cgccgaggcc tatcttatca atccccgcac
    13201 gccccagccg atccggcgtt tccagccgcc agaagaagtc ctgttttttg cctttcaaat
    13261 gatggcgggt ataagtcgcc tcgtgatatg tctcctgata aaccatcacc ccatccagac
    13321 caagttgctt taactcggcg tattccgtct ccgccagcgg ttgcacttcc atttgtagtg
    13381 aagagaactg ttcacgcagg gcggggagat gacgacgaaa gtaatccatc cccactttcg
    13441 cctgatgttc accagtgact aatagcagat gttcaaagcc catctcccgt atagcggcgc
    13501 tttccctggc aatatccgct tcatccagcg ttttgcgctt gatgcgatta ctcatggaaa
    13561 atccgcagta cgtgcagttg ttagcgcaaa gattggaaag ataaagcggg acgtagaaac
    13621 taactgtgtt gccaaatcgc tgacgggtca gacgctgcgc ctgttgggcc agttgttcca
    13681 gatagccact ggcggcaggc gataacagcg ccatcatgtc gtcgcgggtg agttgcgagg
    13741 catttagcgc ccgctctacg tcagcagccg ttttgccgtt gatacgcagg cggatgtcgt
    13801 cccagtccag ttgtcgccag cgattgctga aggttttcat gccgatgcct ccagaaatcc
    13861 ggtcagcggg ctggtggcat gagcaaaata actgcggctg cccggtccgg actgacgtgc
    13921 cagcaggcct gcttctaccg ccagacgaaa tgccttcgcc atgttgacgg gatcgtccgc
    13981 gacggcaatc gccgtattca ctaacaccgc gtcggccccc atttccagcg cctgcgcggc
    14041 atggctggga acgccgatgc cagcatcgac aaccaccgga actgtggcct gctggataat
    14101 aatctccagc atggcacggg tttccagtcc ctgattcgag ccaatcggcg cgccgagcgg
    14161 catcaccgct gcacagccga cttcttccag acgtttacac aataccggat cggccccgca
    14221 gtaaggcagc acgacaaatc cctgttgtac cagcgtttcg gcggctttca gggtttcgat
    14281 gggatcgggc aacagccagc gggcgtcagg gtgaatctcc aattttagcc agtttgtgcc
    14341 taacgcttca cgtgccagat gggcggcgaa aatggcttct tccgctgttt tcgccccgga
    14401 agtattcggc agcagggcca cacccgccgc gataagcggt tcgaggatag cgtcgttgtg
    14461 ctggcgcaag tcgacacgtt tcatcgccag tgtcaccagc tggctgccgg aagcgcggat
    14521 cgcctccacc atcagttgtg aagaagcgaa tttccctgtg ccggtaaaca gatgtgaatc
    14581 aaacgttttg tccacaatac gtaacatctc aaccccctgc aataacctga aaaagcagga
    14641 tctggtcgcc atcctgcacg atatgttgcg tccactgctc acgcgggacg atttgctgat
    14701 taatcgccag agccgcgccc gcttgtcgtt ggtcgagttg ctccagtagt tcgtgaacag
    14761 tttgcccggc ggcgcactgc atcggttgat cgttaaacag gatctgcatt gcttcctccg
    14821 cataccgggc aaccactggc gcggcgcaac gccaggctgc gccactggct cgacttaccg
    14881 tcgaacagtc ggagttctcc cgcaggcgtc tctataccgc ttaataactt aatggcttcc
    14941 agtgcctgca aagtgcccat caccccgacc accgggccaa ccacgcccgc cgtgcggcag
    15001 ttgcgttctg gctgctggtt atctggccac aggcagcggt aacacccctg ctcccagggc
    15061 ggtgtcagta ccatcaactg accgccaaat ccgaccgcgc tggcggtgat aagcggcgtg
    15121 ttgagtgcca cgcaggcggc attaatctcc tggcgagtcg ccatattgtc ggtacagtcg
    15181 agcaccacat cggcttgtgc aaccgcatct ttcaacgcct cacccgttaa ccgttgttgt
    15241 aatgctgtca gttgaatgtc gggattcaac tgtgtcagtc gctgttggct gacctgcgat
    15301 ttcgggctgt tgatatcttc agtggtaaag aggatttgtc gttgcagatt gcttaaatgc
    15361 acatcgtcgt catctgccag taccagcgtc ccgacgccag cccctgccag gtatagcgca
    15421 gcaggtgtac ccagcccgcc aagaccaata atcagcacct ggctgtcgag cagtttttgc
    15481 tgcccgtcaa gagcgatatc gtcgagcaga atttggcggc tataacgcat aaagtcacgg
    15541 tcattcatcg ccaactcctg caatttccag caactgtgcc gttgccaaac gccagtctgc
    15601 ggcttgagta atggcgctga cgacggcgat actgccgaca cccgttgcta tcaccgcagg
    15661 cgcgcgtgcc agactgatac cgccaatcgc cacggtggga taatccgcca ggcgctcaac
    15721 atgccgtgcc aactgttcca gcccctgcgg tgcagaaggc atctgtttgg tttgcgtcgg
    15781 gaacacatgt cccagcgcga tataagaggg gcgtgctgcc agcgcgacgt cgatttccat
    15841 atcgtcatgt gtcgaaacgc ccagccgcag gcctgccgcg cggatggcat tgagatcggt
    15901 ggcttgcaaa tcttcctgcc ccaaatggac gccatacgcc tgatgcttga tcgcgagccg
    15961 ccagtaatcg ttgataaaca atcgcgcgtt atagcggcgg cccagcgcaa ttgccgccac
    16021 gacatcggct tccacctctt catcgcgccg atctttgatg cgtagctgga gagtacgtac
    16081 gcctgcatcc aacagacgtt cgatccactg tacgctgtcc accaccgggt acagtcctaa
    16141 acgaaaaggt acaggaggaa aatcaggctg atacatcacg cttcctcctt atgcaggtag
    16201 atttctccgc ctctggcacg gaagttctcc gacatatccg ccattcccac ttcaatggtt
    16261 tgcgcggcgg cgtaatcgcg tacttcctgg ctgattttca tcgagcagaa ttttggccca
    16321 cacatggagc aaaaatgggc gactttgccg gactcttggg gcagggtttc atcgtgataa
    16381 gcgcgggcgg taaacgggtc gagggccaga ttaaactggt cttcccagcg aaattcgaag
    16441 cgggctttcg acatggcgtt atcgcgaatt tgcgcgcccg gatgcccttt tgccaggtca
    16501 gcagcgtggg cggcaatctt ataagtgata agcccctgct taacatcttc tttattgggc
    16561 agacccagat gctcttttgg cgttacgtaa cagagcatcg cgcagccaaa ccagccaatc
    16621 atcgccgcac caatccccga cgtgaagtgg tcatagcccg gtgcaatatc ggtagttagc
    16681 ggccccagag tgtaaaacgg cgcttcgtgg cagtgctcta actcctcggt catattgcgg
    16741 cggatcatct gcatcggcac atggcctggg ccttcaatca tcacctgtac gtcatattcc
    16801 caggcgattt tggtcagttc gcccagcgta tgcaattcgg caaactgcgc ttcatcgttg
    16861 gcgtcctgaa tagaaccggg gcgcagaccg tcgcccagcg acagcgaaac gtcataagcg
    16921 gcacagattt cacaaatttc gcggaagtgt tgatagagga aattttcctg atgatgggag
    16981 aggcaccatt tcgccataat cgaaccgccg cgagagacga taccggtcag gcgtttcgcg
    17041 gtcatcggca catagcgcag cagtacgccc gcatggatag tgaagtaatc cacaccttgc
    17101 tcggcctgtt ccagcagcgt gtcgcggaac gcttcccagg taagatcttc ggcgatcccg
    17161 ttaaccttct ccagcgcctg gtagatcggc actgtaccga tcggcaccgg actgttacgc
    17221 aaaatccact cgcgggtttc gtgaatatag cgaccggtgg agagatccat caccgtatcc
    17281 gctccccagc gcgtggacca taccagcttt tccacttctt cttcgatgga agaggtgacc
    17341 gccgagttgc cgatattggc gttaactttt accaggaagt tacggccaat aatcatcggc
    17401 tccgattccg gatgattaat gttggccggg ataatcgcac gtccggcagc aacttcatca
    17461 cggacaaatt ccgcagtgat attttccggc agatgtgcgc caaagctcat tcccggatgc
    17521 tggtggcgta aaacctcgct acggatgcgc tcgcggccca tattctcgcg gatggcgatg
    17581 aattccattt ccggcgtgat gatgccctgg cgggcgtagt gcagttgggt gacacggcgt
    17641 cctgttttgg cgcgttttgg cgttaataag ccgctaaaac gtagctcgtc gagaccatca
    17701 tctgccagcc gcgttctggt gtaatcggaa ctgcgcacgg taagttcttc ggtatcggcg
    17761 cgcgcatcga tccacggctg gcgtagtttt gccagccctt gctgcacgtt aatggcgatc
    17821 tgcggatcac catacgggcc ggaggtgtcg tagaccggaa tcgcttcgtt ttcttcgtac
    17881 tgcggctgtt ctttgctacc gccaattagc gtcgggctaa gctggatctc acgcatcggc
    17941 acgcgcacgc cggggtgtgt gccagtgata taaatgcgtt ttgagttggg aaaggcggtg
    18001 ccttccaggg tgtcgataaa atgttgggcc cgggcgcgtt gttcgcggcg ggtcagtttt
    18061 gttgcagaca tagctcattc caaaaagtta aggacgtggc ttgtcagacg acggatgaag
    18121 caagagacga tcgccgcaaa ggcgatgcga tagcagatta actcttgttc ccttcgcagg
    18181 tattagcctg atcaggttcc gcggatcccg aataaacggt ctcagccagt taaggcactc
    18241 cgacaagaaa ttagccccca caaacggggc gttgaatgta aattacgcgt taacagcgca
    18301 gaactcaagc aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt
    18361 ccagcacaaa gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa
    18421 ggtaattatc atgatcgatg gcggtttcca ggctggaatc gtagtaatcc ataatctgtt
    18481 gggtattggc ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt
    18541 ccagcttatg aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca
    18601 ggctctgaca aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag
    18661 gcttaatgcc aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc
    18721 gatcaaccag tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca
    18781 tgattgactc cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt
    18841 aaacatgcca gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag
    18901 atcacggctg gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc
    18961 catatggcga agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat
    19021 ggcaggggga acctgtttgg ttaatacaac agcagcggcg tcacgatatg gggtcggtac
    19081 gtcaggtcat tgatctcgat gttgggctgt ttcaactggc cggacgtggc gtgcaactgg
    19141 cggagtttta ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca
    19201 aaaccgaatg ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc
    19261 cctgcattat tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc
    19321 atcgtaacgg tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc
    19381 aggcagtcgc gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg
    19441 tgacttctca gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg
    19501 acagcggcga catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg
    19561 acgatttgcc gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatactg
    19621 tggcgatgtg tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa
    19681 cagccaaaat gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg
    19741 ttgatgtcac tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag
    19801 ccacgcgcgc ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg
    19861 aagtgacttt gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata
    19921 tcctcaccgt gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc
    19981 gttttacctc gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg
    20041 aagatgagct gggttacgtg atgaacgcgg tgcgtaccat tcgtcacgaa ctgaaaggcg
    20101 aagtgccgct gattggtttt tccggcagcc cgtggacgct ggcaacctac atggtggaag
    20161 gcggcagcag caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc
    20221 tgcacgctct gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta
    20281 aagccggtgc tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg
    20341 attatcaaca gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa
    20401 acgacggtcg ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag
    20461 ccatggctga aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg
    20521 cgcgccgccg tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt
    20581 acgcgccgcc tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg
    20641 gcgaaggtca tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg
    20701 ctggcgtgtt cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taagagcctg
    20761 atccatcagg ctattttatt tgccattttg aggccgggca gtgctcaaaa tcctcacgta
    20821 ctacgtgtac gctccggttt ttccgcgctg tccggcctca aactgccttc aacaattacg
    20881 cctgttgaac caggctctaa ggagcgatta tggatctcgc gtcattacgc gctcaacaaa
    20941 tcgaactggc ttcttctgtg atctgcgagg atcgactcga taaagatcca ccggatctga
    21001 tcgccggagc cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc
    21061 tgctgaaata tccctcgctt gagctggtgg agtataaagt tgcccgcatc gccaccacca
    21121 tgccttacat tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg
    21181 agatgctgtc gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc
    21241 gccgtcttgg cgtcgccagc cattttggct tattggtgga tgtgccgacc attggcgtgg
    21301 cgaaaaaacg gctctgcggt aaattcgaac cgctctccag caaaccgggc gcgctggccc
    21361 cactgatgga taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc
    21421 cgttgtttat cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac
    21481 gctgcatgaa aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg
    21541 aacgtccggc gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata
    21601 atttccgtat ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc
    21661 tggaaagctg gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt
    21721 acgccatgtt ctgccagcaa accggttttg gtgatgggca aatttaccgt cgtattctcg
    21781 atctcatctg ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg
    21841 agaaatttga agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg
    21901 caatcgatgc ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc
    21961 tcgaacacgc ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga
    22021 ctcaggctgg tcgcgaaatg agcgatgaag agcttaaaga aaacccagct gtagagcaag
    22081 aatgggacat tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg
    22141 agctgataaa aggccttagg gcagacctgc gtgagtcggg tgagagcaat attggtataa
    22201 tttttcagca ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct
    22261 tccctttcgc cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg
    22321 tgtatgcagg agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa
    22381 acacattgta aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag
    22441 cagaactgtc caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg
    22501 agtctctgaa agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc
    22561 atcgcgctga gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag
    22621 ctaacgtacc ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg
    22681 tggcagtgaa cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat
    22741 cattgatgct ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct tcacttcctc
    22801 cctttaccgc cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca
    22861 gcggcgataa tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga
    22921 cgcgagagta tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca
    22981 aaccgccggt gaacattcgc gcgcgttttg acgatcgcgg cgatctgagc tttatgcaac
    23041 gtgaatccga tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc
    23101 gtgctgatca gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc
    23161 aggggcgctg gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac
    23221 ctgatttaga ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg
    23281 atgtcagcgt ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact
    23341 ctgatttctg tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc
    23401 catatgaccg ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat
    23461 acccgcttca gccatcgcga tatctcgttt cacccgtaac tcatctaacg actgacgcaa
    23521 attctccatc tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa
    23581 cagggcattg tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagc
    23641 gtaaaagtca ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc
    23701 attttgctgc cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat
    23761 tgctgaaagc gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc
    23821 agtggttgtc ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac
    23881 gggtaatctg gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt
    23941 tttactcatt gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg
    24001 gcatgatttc tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa
    24061 cgttctaaag actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg
    24121 ctggtagggt tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc
    24181 agacaaacgc tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc
    24241 gtcggcatgg ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc
    24301 gggcagcctg gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat
    24361 agcaactccg gcatggtggg aaaacagctt tattcccctc aggaaatgca gcagttacat
    24421 ccgggagatg aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa
    24481 atttatcgcc agtttcaacc gatgtttgct gctggaatgc accggatgcg ccatatgcag
    24541 cagtatgccg cgacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt
    24601 gccgaagatc gtgagcagag aaacaccctg attattctct tcgccctggc gacggtcttg
    24661 ctggcaagcg tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta
    24721 caagatgaaa tgaagcgcaa agagaagctg gtggcgctag ggcatcttgc ggcaggcgtt
    24781 gcccacgaaa tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag
    24841 cgcgcgcctg cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac
    24901 cgtttaaacc gcgtggtaag cgagttgctg gaactggtta agccaacgca tctggctttg
    24961 caggcggtgg atctcaacac gctgattaac cactcattac agctggtaag ccaggatgca
    25021 aacagccggg agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc
    25081 gatccggaca ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt
    25141 ggtcagcatg gcgtgattag cgtgacggcc agcgaaagcg gcgcgggcgt gaaaatcagc
    25201 gttaccgaca gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac
    25261 ttcaccacca aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa
    25321 caacacggtg gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc
    25381 tggcttccgg tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata
    25441 ttctggtggt ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct
    25501 ggggctataa cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggaac
    25561 aggtttttga tcttgtgctt tgcgatgtgc gaatggcgga gatggacggc atcgccacgc
    25621 tgaaagagat caaagcgtta aacccggcaa ttccggtgct gattatgact gcgtactcca
    25681 gcgtcgagac ggcggtagag gcactgaaaa ctggggcgct ggattatctc atcaagccgc
    25741 tggatttcga taacctacag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg
    25801 atgctgaaac acctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga
    25861 tgcaacacct gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc
    25921 acggcgattc cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac
    25981 gtagcgaaaa accgctggta acgctcaact gtgcggcact caacgaatcc ttgctggaat
    26041 ctgaattgtt cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc
    26101 gctttgttga ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga
    26161 tgatgcaggt gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca
    26221 accagactat ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag
    26281 aggtgaatgc cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg
    26341 aagtaccatc gctgcggcaa cggcgggaag atatccctct gctggctggc cattttcttc
    26401 agcgctttgc cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt
    26461 tgctgatcca ctacgactgg ccgggaaata ttcgtgaact ggaaaacgcg gtggaacggg
    26521 cggtggtgct gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca
    26581 gtacgccgat cccgctgggg caaagtcagg atattcagcc gctggtggaa gtggaaaaag
    26641 aggtgattct ggcggcgctg gagaaaacgg gcggcaacaa aaccgaagcc gcccgtcagt
    26701 tagggatcac gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga
    26761 tggcgcgcca accgatatct ttccggcaga agcaatcatc ccagtggata tcggtcatta
    26821 aggcgtaggc gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta
    26881 cgcgcccgcc gctggttacc acctgctcgt catccgccag ttttgtgccc gcgtggaaca
    26941 ctttgccgtc agccacttct tccagcggca ggccgtggat cacatcgccg gtgcggtaat
    27001 cgcccggata tccacccgca gccatcacca cgctgagaga agcacgttca tcccactctg
    27061 acgttttctc gtccagtttg ccttcacagg ccgccaggca gagctcaacc agatcggact
    27121 tcatgcgcag cataatcggc tgggtttccg gatcgccaaa gcggcagtta aattcaataa
    27181 ccttcggatt gccctgtttg tcgatcatca ggcccgcata gagaaaaccg gtgtaggtgt
    27241 tgccttccgc cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat
    27301 gaacttcatc ggttactacc ggcgcggggg agtaagcgcc catcccgccg gtgtttggtc
    27361 cgttatcttt atcgcctacg cgtttgtgat cctggctggt agccatcggc agcacatgct
    27421 cgccgtccac catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga
    27481 tgcgatgacc cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg
    27541 cttcttccag cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa
    27601 tgacgattgg cgcgcctttc tcacgcagat acaccagcgc aggttctacc tcggtgaagt
    27661 tctggtattc cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg
    27721 agccttccag ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga
    27781 aggtatcgac cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct
    27841 tttcgttttg tgcgaaatcc aacagcgccg ggatatcggt cacgccgata gcaacgtttt
    27901 gcagcgcggg ttccagcgca gtgcctgcat tacccggagc aacaaaaaca gtctcaacca
    27961 gcggcgactg ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaatcacta
    28021 atactttcat ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc
    28081 aataccgtgc tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg
    28141 gattacgcag gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa
    28201 cgcgtcagaa gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc
    28261 ggcgattttc gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt
    28321 gtttttggca tagacgatag cattggattt cacgaatttc gccactttcc agcagaacag
    28381 cgcatcacgc agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc
    28441 accgaccatc cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc
    28501 aagacccgga acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc
    28561 ggcggtgatt ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg
    28621 acgagaaatg atggcctgtg cggtttccgc atccagctcg cggttaaagg caatgatgcc
    28681 gccgaatgcg gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt
    28741 gccgatagct acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa
    28801 ctctttcacg cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt
    28861 accctgaacc tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa
    28921 ggcagcctgc tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag
    28981 gttcagcgtg cgtgggaagc gaccggcggc ttctttgctt tcaccgtggt acgccggaac
    29041 catgctgccg aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt
    29101 gatggcgagg tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt
    29161 aataatggcg tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc
    29221 ggcggagcgc accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga
    29281 gcagccttca cgagcaacag tctgggcgaa cggatacagg ttaacaacca ccatatcgat
    29341 aggctggatc tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc
    29401 accatgtact ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt
    29461 gtaatcggaa acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc
    29521 tgtagacagc agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc
    29581 tttgtcagaa acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc
    29641 cctgggtttg actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg
    29701 cccctaacgg ggcatcctta tttttcgccc gcattgtaac gaaaacgttt gcgcaacgct
    29761 cgcgaatttt tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat
    29821 acagaaatta cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt
    29881 ttgctgggga atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag
    29941 aactccctat aatgcgcctt catcgacacg gcggatgtga atcacttcac acaaacagcc
    30001 ggttcggttg aaga
//
LOCUS       DADLZM010000036        29733 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLZM010000036 DADLZM010000000
VERSION     DADLZM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.036"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            347..640
                     /locus_tag="JNY73_001589"
     CDS             347..640
                     /locus_tag="JNY73_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047640.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JNY73_001590"
     CDS             712..1371
                     /locus_tag="JNY73_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY8047641.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1448..1909
                     /locus_tag="JNY73_001591"
     CDS             1448..1909
                     /locus_tag="JNY73_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY8047642.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNY73_001592"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNY73_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /gene="umuD"
                     /locus_tag="JNY73_001593"
     CDS             3389..3808
                     /gene="umuD"
                     /locus_tag="JNY73_001593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY8047643.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5076
                     /locus_tag="JNY73_001594"
     CDS             3808..5076
                     /locus_tag="JNY73_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY8047644.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSTSVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JNY73_001595"
     CDS             complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JNY73_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY8047645.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JNY73_001596"
     CDS             complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JNY73_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY8047646.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7560..8279
                     /gene="fadR"
                     /locus_tag="JNY73_001597"
     CDS             7560..8279
                     /gene="fadR"
                     /locus_tag="JNY73_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY8047647.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8331..9863)
                     /locus_tag="JNY73_001598"
     CDS             complement(8331..9863)
                     /locus_tag="JNY73_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY8047648.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARHYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10193..11491
                     /locus_tag="JNY73_001599"
     CDS             10193..11491
                     /locus_tag="JNY73_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY8047649.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11501..12571
                     /gene="dadX"
                     /locus_tag="JNY73_001600"
     CDS             11501..12571
                     /gene="dadX"
                     /locus_tag="JNY73_001600"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY8047650.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12739..14165)
                     /locus_tag="JNY73_001601"
                     /pseudo
     CDS             complement(12739..14165)
                     /locus_tag="JNY73_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14775..16511)
                     /locus_tag="JNY73_001602"
     CDS             complement(14775..16511)
                     /locus_tag="JNY73_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY8047651.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="JNY73_001603"
     CDS             complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="JNY73_001603"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY8047652.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17620..18231
                     /gene="emtA"
                     /locus_tag="JNY73_001604"
     CDS             17620..18231
                     /gene="emtA"
                     /locus_tag="JNY73_001604"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY8047653.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLRYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18233..18967)
                     /locus_tag="JNY73_001605"
     CDS             complement(18233..18967)
                     /locus_tag="JNY73_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY8047654.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19168..19422
                     /locus_tag="JNY73_001606"
     CDS             19168..19422
                     /locus_tag="JNY73_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY8047655.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19472..21425)
                     /locus_tag="JNY73_001607"
                     /pseudo
     CDS             complement(19472..21425)
                     /locus_tag="JNY73_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(21451..22305)
                     /locus_tag="JNY73_001608"
     CDS             complement(21451..22305)
                     /locus_tag="JNY73_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY8047656.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22305..23114)
                     /locus_tag="JNY73_001609"
                     /pseudo
     CDS             complement(22305..23114)
                     /locus_tag="JNY73_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(23124..23882)
                     /locus_tag="JNY73_001610"
     CDS             complement(23124..23882)
                     /locus_tag="JNY73_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8047657.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APIIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23879..24859)
                     /locus_tag="JNY73_001611"
     CDS             complement(23879..24859)
                     /locus_tag="JNY73_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY8047658.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24859..25881)
                     /locus_tag="JNY73_001612"
     CDS             complement(24859..25881)
                     /locus_tag="JNY73_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY8047659.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWLPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26947..27387
                     /locus_tag="JNY73_001613"
     CDS             26947..27387
                     /locus_tag="JNY73_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047660.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(27466..29163)
                     /locus_tag="JNY73_001614"
     CDS             complement(27466..29163)
                     /locus_tag="JNY73_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY8047661.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLGTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(29483..>29733)
                     /locus_tag="JNY73_001615"
     CDS             complement(29483..>29733)
                     /locus_tag="JNY73_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY8047662.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         7456 a         7226 c         7638 g         7413 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     4561 tcgtttggtg aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct
     4621 gcccgggcgg cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt
     4681 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcac gtcggtaaaa
     4741 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     4801 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     4861 agtcagggag tcgcgcagct caatttattc gatgacaatg caccgcgccc cgggagtgag
     4921 caattgatgg cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt
     4981 gccgggcagg ggatccaaca gcaatggcag atgaagcgag ctatgctttc accacgttat
     5041 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgtt
     5101 cccgcaggag cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg
     5161 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     5221 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     5281 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     5341 acaggtggca aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     5401 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     5461 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     5521 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     5581 tgccagagca gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa
     5641 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     5701 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     5761 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     5821 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     5881 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     5941 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     6001 cgacagaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     6061 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     6121 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     6181 catgttcaga tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa
     6241 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     6301 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga
     6361 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     6421 cgccaataat cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc
     6481 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     6541 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     6601 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     6661 tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac
     6721 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat
     6781 gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc
     6841 gatgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     6901 catcgaggaa cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt
     6961 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     7021 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacccctt
     7081 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     7141 cggggagtag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     7201 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     7261 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     7321 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgtttttg
     7381 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     7441 aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt
     7501 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     7561 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     7621 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     7681 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     7741 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa
     7801 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     7861 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc
     7921 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     7981 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     8041 acggtctgat ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg
     8101 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     8161 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     8221 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     8281 cccttccgtt taaagagcaa acccctcaaa cgaggggttt tttgttgttt ttacagattt
     8341 cccattcttg gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg
     8401 agcatcacat caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga
     8461 tccagcggtg cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa
     8521 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgataac
     8581 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     8641 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga
     8701 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     8761 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     8821 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     8881 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     8941 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag
     9001 aaggtcgcac agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     9061 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     9121 tccataaaat agagcaggtt ttcttgtggt tcggacggat agtggcgcgc ttcagcaacc
     9181 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga
     9241 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     9301 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     9361 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     9421 tcgacaatcg aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag
     9481 ttatgcccat agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc
     9541 atcaagtaag cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga
     9601 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     9661 ttaattggca tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca
     9721 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     9781 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     9841 ttaatagaat cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga
     9901 gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct
     9961 ttgcgatttc ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga
    10021 taaaaagggc gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat
    10081 gtgagtcagc tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa
    10141 gatgtaatta gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt
    10201 tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg
    10261 acatgaggtc accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa
    10321 tgccgggcaa atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa
    10381 agcgattaaa tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca
    10441 gtttcagttg aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga
    10501 aaacaaaggg cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg
    10561 cgccgaaacc aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac
    10621 cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc
    10681 gtatcagctg ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc
    10741 gcacaaactg acgggcggcc tgcagttacc caatgatgaa accggagact gccagctgtt
    10801 tacccagaat ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc
    10861 cgttgaccaa ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt
    10921 gattaaggcc gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg
    10981 cattgttgat attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca
    11041 ggaagatggt gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg
    11101 tttcgataac cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct
    11161 gttgcaaccg cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg
    11221 tcatgtcgag caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc
    11281 ggttgtcggg cgtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg
    11341 ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc
    11401 agcgatccca tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg
    11461 tccgggccat ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc
    11521 cagcctcgat ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca
    11581 cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg
    11641 gagcgcgctc ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt
    11701 acgtgagcgc ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga
    11761 tctggagatt tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa
    11821 agcactgcaa aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg
    11881 gatgaatcgg ttgggcttcc agcccgatcg cgtgcttacc gtctggcagc agttgcgggc
    11941 aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga
    12001 tggaatttcc ggcgcgatgg cgcgtattga gcaggcggcg gaggggctgg agtgtcggcg
    12061 ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg
    12121 gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac
    12181 cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc
    12241 gggcgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat
    12301 tgtcgccgca ggatacgccg acggttatcc gcgccatgcg cctaccggta ctcctgtttt
    12361 agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tggcggttga
    12421 tttaacgcct tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat
    12481 caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcgct
    12541 ggcgttacgc gtcccggttg tgacagtgta acttattgta agccggatcg gaggcagcgt
    12601 cttctggttg cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac
    12661 agagccattg cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga
    12721 actcgccgca caggcgttct acataaaacg cttacgcttc attattgact cggagtcgac
    12781 ttagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta
    12841 acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc
    12901 tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga
    12961 tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa
    13021 aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc
    13081 gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg
    13141 ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg
    13201 ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga
    13261 gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt
    13321 ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta
    13381 atcactctca ggttagtcga cagctcaggc agccagataa tacagttttc atagcacttc
    13441 agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag
    13501 tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg
    13561 cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg
    13621 ctaagattga gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct
    13681 gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct
    13741 tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct
    13801 tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc
    13861 cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag gcgagtcccc
    13921 aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca
    13981 atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc
    14041 tgcaaaattt gattcttgag ttccaccgac aggaagagcc gccctggcgg gcgtcgtacg
    14101 tctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc tttaaagcta
    14161 gccataatgc tacgtttcct tgaaataaat atcctctggc agcgccggag acgtgaatgt
    14221 ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc atcgttgact
    14281 ttttccctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg gcctaggtag
    14341 ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt tattaagcaa
    14401 aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga aacgcctatt
    14461 gtgtgggcta tgtcagtgcg gcaggttgcg tggggaatat gttcaggcga tgtcaggcag
    14521 gggcccctga tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa
    14581 aaagccctta cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa
    14641 tggtttgtca tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg
    14701 aaatgacaag tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag
    14761 atgctgcatt aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac
    14821 acttcattat cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca
    14881 ccgacaaccg gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta
    14941 tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg
    15001 ctggcttcga gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg
    15061 ctgaacagtt taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg
    15121 ccttcgcgca gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct
    15181 gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta
    15241 tcagcactca attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca
    15301 acgcgtgaca ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac
    15361 gagagtgatg ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag
    15421 aacagtcgcg cattctccag ccccgccatc atcgggaaca cggccaggat gattggcacc
    15481 gcgccgcgta atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa
    15541 gggagcaatc cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa
    15601 atgagcgccg gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc
    15661 aggaacatgg cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag
    15721 cggttgcgaa tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg
    15781 ctgccttcca gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat
    15841 ggatataatc cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg
    15901 ccaagaccga tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa
    15961 ctgacactgc tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc
    16021 attggatcat tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc
    16081 ttaccaccca gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt
    16141 aataagcctt caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca
    16201 gaggtgataa gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag
    16261 gaactggcct gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt
    16321 accatgtagg cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc
    16381 agcatgccga tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa
    16441 ctaagtaata tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt
    16501 gtggcatcca acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat
    16561 tagcggcaga gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac
    16621 aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg ttattcagaa tggcatgtgc
    16681 gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg
    16741 aatcgacagg cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata
    16801 atcattgggc gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat
    16861 tcccgcatga tagagctgca acagcatacg ttcgacgcgg aaagggtgct cgttaatatc
    16921 ttccagcacc agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat
    16981 cagcatcgca agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc
    17041 ttgccattca atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa
    17101 ggcgttcagt tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac
    17161 attgccttgc gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag
    17221 taacgggtca tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg
    17281 actggcaccg taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc
    17341 aagggaattg agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg
    17401 ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg
    17461 cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat
    17521 ggcaattcct tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg
    17581 tcacctcaac ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc
    17641 tttttgattg tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg
    17701 aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt
    17761 gcagcctggg gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt
    17821 aatccgaacg cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc
    17881 tccggacgtg atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg
    17941 aaaaatccag agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc
    18001 ccgctggcag gcattgaaga tccgaaggta ctgcgatatg cgctggtggt gtcatacgct
    18061 aacggggcag gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa
    18121 atcaacgatt tggatgctga tgagttcctc gatcacgtag cgcgaaatca tcctgcgccg
    18181 caggctccgc gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc
    18241 gcactttgtc cgctttttcc cgtgcttctc gctcgagaga gaaaataatc cgctgtaatt
    18301 gccgctccac cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg
    18361 tttcattctt gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt
    18421 gaaaaacacc ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta
    18481 attcggcagg ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca
    18541 agcggaaacg taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga
    18601 gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta
    18661 cggtaataaa tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact
    18721 cgactttcgc accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga
    18781 tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta
    18841 ttttgctgat cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca
    18901 aatcgcgtaa cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat
    18961 gactcacgcg gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac
    19021 aaaacttaat acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac
    19081 gcccagcatc gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga
    19141 ccgattcatc gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg
    19201 atagccggca ttatcgccaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg
    19261 acctgtattc tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc
    19321 attggcggct ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct
    19381 atcctggtgc tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat
    19441 agcgatacag atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga
    19501 aattgcgacc ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat
    19561 tgaccgacgc attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca
    19621 ccgcaaaacc ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct
    19681 cttccgacag cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg
    19741 tctgcgttgg aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca
    19801 gagcgtagct caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca
    19861 gaccgctgta atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt
    19921 gcgagaggat ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc
    19981 gcgtgaaggc accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgtg
    20041 ggttaacaat cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg
    20101 tcggaaagcg tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt
    20161 atttccccat cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc
    20221 tgccatcttt ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg
    20281 ccacgacatc gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac
    20341 gatcttcata gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt
    20401 tccagttaac ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc
    20461 cggcaccgtc gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag
    20521 ccagcgagtt gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca
    20581 gggtaaaacg gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact
    20641 ctggccactg ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt
    20701 tttcaccatc ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac
    20761 cgagcttcac aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc
    20821 ctgcaatatc attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac
    20881 cgctgacttg caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg
    20941 cattgtcctg actacggctc catccctggc gatatcccag acttgcttct tcagggtggt
    21001 gatattaatg gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga
    21061 aacttcgact gcccccagat tgttggtcag aacccgcgcc agatcgaggt tgccgtcata
    21121 gggaacataa atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg
    21181 aacttttacc tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac
    21241 acttaatgcc tgagcgacat tctgtttatc caaggctttc atggtgggtt gatcgagaac
    21301 ggtggtcgtc gtcgatgata ccggactgga ccatacggtc agcgtgtcgc ctcctgagcc
    21361 gctacggcct gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt
    21421 ctcatagaat aaattccgtt taacaaagaa ttaaatagag gctgtgggtt gcagactgac
    21481 tttaatgtca gctggtgccg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca
    21541 gtcgaggtaa tttttgagtg tcgtcagatt aattccgccg gtcagagcca gcgtgcagtg
    21601 aggagccagc gacggcgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc
    21661 gagctgaagt acgtctggtt gtgcgcgtaa cgcggcgata gcctctttcg gcgtgtcggc
    21721 ttcaacaaca attttcttct cgggtgcgtg gcgacgtaac tgagcgattg cgcctgacca
    21781 gccctgattg tcatgaagaa aatggcggtg gttggcaaac aataatatgg tttccgcaca
    21841 tccggcacga tgaattagtc ctccggcagc cagaattgcc tgcgaagcca gtaagcgggt
    21901 gcccggaatt gcttttcggg tgcaggcgat attgccgtca ggataacgtt cacgaagcaa
    21961 tgccagcatt tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac
    22021 tgccttccat ccctgatgaa gtgctgcggc attaccctgc gcacggatta gccgttgacc
    22081 cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa
    22141 catcttacac gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa aaaacgctat
    22201 atagccaggc tggtgtccaa tatttaatgc tcgcgtggtc aggtcacctc cctggatatc
    22261 ttccagcagt aacgcatcga tttgtgcctg agaaaggaaa atcatcacag tgcctcctgc
    22321 ggtacgctat cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca
    22381 tcttgttgtt gataccagtg atataaccgc tcacgctcat cgtcgttaag cgcgccaaga
    22441 ctccagctga cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt
    22501 ccacggataa aatcaacgtg ggcataaatt cccatctgat acaggacgtt gagcgcaaag
    22561 atatagttgg gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg
    22621 aacgaggtgc tgaccagatg agttgtatag acgcgaagcc gtgcctgatt attcagtttg
    22681 ctcattgcat ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag
    22741 cgtggcaggt cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtcagcc
    22801 ttcagtgcgg cggcgcggcg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca
    22861 taaaccgtag tgagtttgtc agccagcgcc agcgatacgg tgcctggtcc gcagcccata
    22921 tcgaataagg tctgagcgcc ttgcaggtca attttagcga tcagttgttg caggtagctg
    22981 tccgttggcg aggctcaatt ttccgccatt ttctccgccc gttgatccca gtgatcgggc
    23041 gttttttctg tgcggtgagc cagtttgagt tgctgcagat aaaggtctgc aaaatcaata
    23101 tcatcaatga gcatgattgt tccttagcgg ctaattgcag agaggtgatg atgaatttgg
    23161 tcggcagaaa cccgatacag ggccgccagt ttgttcgttg ttaactgttc ggtcggtaag
    23221 ccttgtgtta ctcgtccatc tggctctacc tggataatgc tgtcggcaat ggcgttggca
    23281 tggagcggat ggtgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc
    23341 gtgtccagaa gttggatctg atgaccgaaa tcgaggctgg aggcgggctc atccagtaac
    23401 aacaagcgcg gttgttggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg
    23461 ctaagcgtat tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc
    23521 gtggctttca ggcgttcttg cttaccggga acggaaaaag cgcctatgat gggggccagg
    23581 cccatcaaca ccatatccag cacactgaaa gcaaaaatac cgtcgtgcgc ctgcggcacc
    23641 caggcaatag cctgtgcgcg ctggcgatgg ctgagtgtct gcacagggag gctatccagt
    23701 aggatctcgc cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg
    23761 ccgcaaccat tcgcgccaag cagacaacaa attgttcctt gcgacagggt gaaggacacg
    23821 tcgcgtagca ctggatggcg ggagtaaccc agagacgcat gacgaacggc aagcaaagtc
    23881 actggcgacc tccgcgtaac aacaaaagca ggaaaaacgg tgcgccaaca aaagcggtca
    23941 gaatgccaag tgggatttct gttgttccaa tgctgcgcgc cagcgtatcg gtaagcagga
    24001 gcagaatagc gccagtacac attgccatag gcaacagctg ctgatggtta tgaccggtca
    24061 acaagcgggc aatgtgcgga acgaccaggc cgatccaacc aattatgccc gctattgcta
    24121 ccgtgctggc ggtgattagt gtggcgcaga caatcaatcc aaagcgcaga cgcgtcacgt
    24181 tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga
    24241 gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta
    24301 aggtcacggt ggacagtccc cccagcaacc agaaggtaat tgacggtaac tgggtgtaag
    24361 gatcggccag cgttttaatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc
    24421 ctaccagcac cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga
    24481 cgcccgcgac caccatcagc tcgccgcaaa atgcgtacag ctggatgtaa acaatgggta
    24541 atccccacag gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat
    24601 ccggtgaaac taagggatta cgcaacattc cctgataggt ggttccggct cctgccagcg
    24661 ctgcgcccag taacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt
    24721 gttcctgcac agacacattt tcttgacgaa gaattagcgc cagcagttgc tgcatatcga
    24781 gatgataagc cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac
    24841 atccgtttac gattctcatt agctcgccac caatttttga tagtcggcat cactgagtga
    24901 acaatgccag aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc
    24961 gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc
    25021 atcgagccag ccaaagggca agccacttaa aaataagatg cgttgttccg caacggcttt
    25081 cacgccctgc cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag
    25141 aataatatcc ggcagccagc gcaggagatt ttccatggaa acctgtgtca ggccgtggcg
    25201 atcggctatt tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga
    25261 accctgtaag cccgtttcca gccctcgggg accgcgcgca gcataaaagc ggaggttagc
    25321 ggcgggtgag gtggcgaatg cctgagcttc accaacgaag tggctggcga gattggcttg
    25381 ttctgcggcg cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga
    25441 ttgcgccagt tttccgttaa gcaataacca ggggatttgt gtctgttcgc taacctgccg
    25501 tgcctgggag atcaaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa
    25561 cgccataagc ccttccagcg atagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg
    25621 taactggcga atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga
    25681 aaaaccgacc attttttctg gcgcgacagc cagcaatagc aaatcggcgg gcgcgcctgc
    25741 gctgacgatg cgtgtaatgt tttgggcttc gggaagagat tgcgaagcaa aacgattaac
    25801 tgtttgtgaa aaggacggta gagactgcag gagcagggta gatgcaagtg cctgcgcaaa
    25861 cgtccgtctg gttatgggca tggtgaacat cctgaaaagc aaaatcgata tatactttca
    25921 tatatatcga taaaaatcat gttcaatatc aaagtttagc tattgaaaac atgaagatgt
    25981 gacgacttcc gcaagcctgt tgaccctctc tatcattaaa aatgtattta tcaggtggct
    26041 aatattattt aaatcattta aggctaaatc atttaaatat gaatgacatt attgtttttt
    26101 atcgtccacg gttacgcgac gttgaagtta cttgttttac aatgagcgat ttttgatatc
    26161 tcgccgagaa aattatcagc attctctcgt ttcagtgtcg tgaatatact gttaacggag
    26221 gtgaaaagaa tgctgggggt tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt
    26281 gctgatagta agcagcgtaa ccagaagtta ccgcaacaag actttgagga ttttaagaac
    26341 gacgttatta atgtcattaa caacaatgaa gatatttatt ttcttagaga gagtaattta
    26401 ctgaatgcgt ttattcgaac gtgcaaaaaa agagatgata acagttacgg aaatatgata
    26461 tttacgcttt attttgataa cgatatctat gggaacagaa ttatcaaagc atcgctgaac
    26521 ggcggagagt tgcagttatt aagcacaggg tgtagcaatg ataacattct ccattttatt
    26581 aatgcatttc tcattcgggt gaaatttccg ctacctggac gcataaaacc ggaactctcg
    26641 ccaaaagccg ggcactccga aggaattgac gcgttgcgct gctctgtaac ggcatttacc
    26701 gcaagccatt tatttcgcca gcgacaatcc gcagagaacc aaacgcatca ggaaacgact
    26761 gaacacaata aatactgtat tgatcaacat attatactgg cacctccagc acgcagtgag
    26821 gatacactgt atcatcagcg aatcttatca ctgcggagct ggttgaacga ctttctgagc
    26881 gaaataagtc ggttcccggt aacaacaaca gaatcctttt ttaattattg tttcgttgtt
    26941 tttggtgtga ttcagaaaat aaatatttta gacagtagcc agtcaggcgt taaaaatggg
    27001 gcggatagtg aggcggtttt ggctgcgctt attgaacatt tagaattgat caatccttct
    27061 ggcagattaa gccaaaatac ccgttcggca atgcttcagt tacgcgaaga ggaatggtct
    27121 gaattctttt tttggttgct aaattctctg gagtgtctgg attatgtgat tattaattta
    27181 actccagaaa gcaaaaaaac gttgatgagt gaacaccgta acaatattca ggtagcaatt
    27241 gatgctttat atagccaacg tcggcgtaaa tcgccgggtg atgaaagtga aacacttacc
    27301 cgtagaaatg acgctatctt cggcaaccac gtatggcaaa catttgcgca atacttcccg
    27361 ccaggacttg aaaaaccgtc tgtgtagaaa aaaccataga tatggtgtta tctgccgaca
    27421 acgccatccg gagaagccga atgacgttgt cggagtaagc gctggttaag gtgtgggttg
    27481 tgcctctttg gttgagggtt gcgtcgttgc tgacttaacg gtcggacgcg tcgccggaac
    27541 attgtcacac ggttgctctt tcgggcagat caaatccagc attttcagcg tcacgccatt
    27601 ggtccagcca aagccatcct gtaatggata ttcgccaccg ccgccccccg ttccggtggt
    27661 gctgacatca tatttttcca ccagcttttt ctcccggtca taggtgtgct gaacattggt
    27721 caggaagtgc cagctaatgt ccatcgccac ctctttttgc ccgtagtttt gtaatccttc
    27781 tgtcgcgacc cactgtaacg gtgcccagcc atttggcgca tcccattgtt gcccactttt
    27841 caccgacgtg gtgttcaggc cgccgggttg cagcagatgt gttttcgtcg ccgtcgccat
    27901 tttgttggcg cgatctttcg ctgccgcatt gacgtacagc gggaacaggg cggccgcggt
    27961 taactgattg cgcactttat gacttttcag gtcgtaatcg gcataccagc cttgttgatc
    28021 gttccacagg tatttttcga tccctttttg acgggcattc gccagcgttt cgtactggtt
    28081 tgccatcgcg ttatctcctg cagctttgct ggcgcgggcg aggatttttt ccattttaaa
    28141 catcaggctg ttcagatcga ccggtacgat gctggtggtg cctaaggtat ttaactgctg
    28201 cgggttgtcc atccagcgcg agctgaaatc ccagccagac gcagcggcag agcgcaggtc
    28261 gcggtaaatt tcagtggcag gtcgattcgg attgcttttg gcggtggcaa tatcttccac
    28321 ccatgactct ggtcgtggcg tatcgcgatc gtcccagtag cggttgagaa gggtaccatc
    28381 ctgaagtttt acaacgcgtt tttcctgttg tccggcttgc aggttttcaa caccgtccat
    28441 ccagtaagca tattcttttt gcatttgcgg caggtattgc ttcaacgcgg catcgccttc
    28501 atgctgcgcc agtaactcta ccatcagggc aaagaagggc ggttgcgagc ggcttaaata
    28561 gtaactgcgg ttgccgttgg gaatatgacc gtaagtgtct atttcatgag caaaattggc
    28621 caccatatcc gcgactttat cccagtgacc gctttcggca agtcctaaca tggtgaagta
    28681 actgtcccag taatatacct cgcgaaaacg tccgcccggc acgacataag gttccggcag
    28741 cggtaacaga gaatcccatt tttcggtgtt ttcggtagaa cgcgttaata ccggccaaag
    28801 tccgtcaata tgttcgcgca gtgactgccc ctctggcgga acatatttct cgccttcttt
    28861 cggcagggtg aaattgacgt taacgaaatg gcgcagatca aatccgctct ggttttgctg
    28921 catccgataa tcagcaagga tcatcagcgg atcgctgttc ggcacggcat cggcaaaggt
    28981 tttttggtcc ggaaaaagtt tggcgttttg cacatcatta aacagcggcc ctaataaaat
    29041 atcaggcggc tgtggtgtta ccggtgtttc ttctgcctgc accgatagcg cagcgaaaca
    29101 caaaaagata caggctggaa ttaacgccat tttttgcggg cgagaaggtg cgggggattt
    29161 catcaatcat tctcctttgg cgaaaccgaa taaaacggta ttcaagtatc agaaaacctt
    29221 agttcaggat cgcactagct gcatgatcga aatctcattc tgcgaacggt caggcatttt
    29281 agaaaaggga attaaaggga taaataacgg cgaaatagag tgatcaaata acgtctgttt
    29341 attagccagc cagcgacatt tggttcgcga ttagcgaaga aaaatcatca taaattggta
    29401 aaaaatataa caggtagtta aaaaacatca gtgctgggta aattgccgga tgacatcaga
    29461 acgatgccat ccgaacggtg gcttaaccct gacggttgaa acgttgcgtt ttaacgtcca
    29521 gcgttagcgt ttcttctggt tgtatcgcat acagtttctc accctgctgg caaatccagc
    29581 caatccccag ttcacgggcg attagggcgc tgtgggatac cggacttccg gcgctaaggc
    29641 agataccttt tacaaccgcc ggatccagtt gcagtactgt ggaaggataa atgttctccg
    29701 ccagtagaat agttggcgag ttaaactgcg gga
//
LOCUS       DADLZM010000037        29418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLZM010000037 DADLZM010000000
VERSION     DADLZM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.037"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..109)
                     /locus_tag="JNY73_001616"
     CDS             complement(<1..109)
                     /locus_tag="JNY73_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAY8047663.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(179..1168)
                     /locus_tag="JNY73_001617"
     CDS             complement(179..1168)
                     /locus_tag="JNY73_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8047664.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAH
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1965..2468
                     /locus_tag="JNY73_001618"
     CDS             1965..2468
                     /locus_tag="JNY73_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY8047665.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2548..2799)
                     /locus_tag="JNY73_001619"
     CDS             complement(2548..2799)
                     /locus_tag="JNY73_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY8047666.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="JNY73_001620"
     CDS             complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="JNY73_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY8047667.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3263..3586
                     /locus_tag="JNY73_001621"
     CDS             3263..3586
                     /locus_tag="JNY73_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047668.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3757..4254
                     /gene="ftnA"
                     /locus_tag="JNY73_001622"
     CDS             3757..4254
                     /gene="ftnA"
                     /locus_tag="JNY73_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY8047669.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4291..4530)
                     /locus_tag="JNY73_001623"
     CDS             complement(4291..4530)
                     /locus_tag="JNY73_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY8047670.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4720..5931
                     /gene="tyrP"
                     /locus_tag="JNY73_001624"
     CDS             4720..5931
                     /gene="tyrP"
                     /locus_tag="JNY73_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY8047671.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="JNY73_001625"
     CDS             complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="JNY73_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY8047672.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6854..6940)
                     /locus_tag="JNY73_001626"
     tRNA            complement(6854..6940)
                     /locus_tag="JNY73_001626"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6904..6906),aa:Leu,seq:taa)
     gene            complement(6953..7026)
                     /locus_tag="JNY73_001627"
     tRNA            complement(6953..7026)
                     /locus_tag="JNY73_001627"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6992..6994),aa:Cys,seq:gca)
     gene            complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="JNY73_001628"
     CDS             complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="JNY73_001628"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY8047673.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="JNY73_001629"
     CDS             complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="JNY73_001629"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY8047674.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="JNY73_001630"
     CDS             complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="JNY73_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY8047675.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10694..10870
                     /locus_tag="JNY73_001631"
     CDS             10694..10870
                     /locus_tag="JNY73_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047676.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10857..11081
                     /locus_tag="JNY73_001632"
     CDS             10857..11081
                     /locus_tag="JNY73_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY8047677.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="JNY73_001633"
                     /pseudo
     CDS             complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="JNY73_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11964..12716)
                     /gene="yecC"
                     /locus_tag="JNY73_001634"
     CDS             complement(11964..12716)
                     /gene="yecC"
                     /locus_tag="JNY73_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY8047678.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="JNY73_001635"
     CDS             complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="JNY73_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY8047679.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="JNY73_001636"
     CDS             complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="JNY73_001636"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY8047680.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="JNY73_001637"
     CDS             complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="JNY73_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8047681.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="JNY73_001638"
     CDS             complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="JNY73_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY8047682.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15972..16691)
                     /locus_tag="JNY73_001639"
     CDS             complement(15972..16691)
                     /locus_tag="JNY73_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY8047683.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDAPQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16856..18508)
                     /locus_tag="JNY73_001640"
     CDS             complement(16856..18508)
                     /locus_tag="JNY73_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY8047684.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18774..20180
                     /gene="fliD"
                     /locus_tag="JNY73_001641"
     CDS             18774..20180
                     /gene="fliD"
                     /locus_tag="JNY73_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY8047685.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20205..20615
                     /gene="fliS"
                     /locus_tag="JNY73_001642"
     CDS             20205..20615
                     /gene="fliS"
                     /locus_tag="JNY73_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY8047686.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20615..20980
                     /gene="fliT"
                     /locus_tag="JNY73_001643"
     CDS             20615..20980
                     /gene="fliT"
                     /locus_tag="JNY73_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY8047687.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21058..22545
                     /gene="amyA"
                     /locus_tag="JNY73_001644"
     CDS             21058..22545
                     /gene="amyA"
                     /locus_tag="JNY73_001644"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY8047688.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22579..22992)
                     /locus_tag="JNY73_001645"
     CDS             complement(22579..22992)
                     /locus_tag="JNY73_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8047689.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23179..24384
                     /gene="yedE"
                     /locus_tag="JNY73_001646"
     CDS             23179..24384
                     /gene="yedE"
                     /locus_tag="JNY73_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY8047690.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24381..24614
                     /gene="yedF"
                     /locus_tag="JNY73_001647"
     CDS             24381..24614
                     /gene="yedF"
                     /locus_tag="JNY73_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY8047691.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24721..25391
                     /locus_tag="JNY73_001648"
                     /pseudo
     CDS             24721..25391
                     /locus_tag="JNY73_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25431..26639)
                     /gene="tnpB"
                     /locus_tag="JNY73_001649"
                     /pseudo
     CDS             complement(25431..26639)
                     /gene="tnpB"
                     /locus_tag="JNY73_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
     gene            26707..27322
                     /gene="tnpA"
                     /locus_tag="JNY73_001650"
                     /pseudo
     CDS             26707..27322
                     /gene="tnpA"
                     /locus_tag="JNY73_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            27650..28732
                     /locus_tag="JNY73_001651"
     CDS             27650..28732
                     /locus_tag="JNY73_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY8047692.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(29221..>29418)
                     /locus_tag="JNY73_001652"
     CDS             complement(29221..>29418)
                     /locus_tag="JNY73_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8047693.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT         7416 a         7406 c         6888 g         7708 t
ORIGIN      
        1 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
       61 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
      121 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
      181 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagta
      241 ccacgtctgt aacttcggta aattttggcg tttcaacgtc ttttgctacc cagttgtaaa
      301 gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc
      361 cggttgcctg tgctttagac agttcgctta ctgcatccac accgttaata ccaataccga
      421 tgatatcggc tgctttaaag ccctgacctt cggttgcacg tacgccgcca agtacggtgc
      481 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
      541 tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga taaatttgtt
      601 tttccgggaa tcctgcagct ttcagtgcgt ccatagaacc cgttgtacgg cggcgggcgg
      661 tatccagttc gttggcggta atcgccatca ctgcgctttc tttgacatcc cagccacgtt
      721 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttagtc gccgccatca
      781 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccactg
      841 caatgacttt catatcgtag ccatgcgctt tcgcgacgat ggcagagccg agtttcgggt
      901 ccggagtaca aataacgaaa ccttttgcgc cactggctgc caggctgtcg atcgcgttca
      961 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
     1021 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagaa
     1081 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
     1141 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta
     1201 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttatcta
     1261 aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca taggagagca
     1321 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa agacataagg
     1381 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
     1441 tggaaatcct tgttgtcttc ggtacccatg cgggatgtct tctttttaac cagtcaatag
     1501 gccgcattac ctggcgttga gtttttgaaa tggtgtaata accacaactc aaagatgtgg
     1561 aaaatgcgcg tcattcattt cgccattaat tatcactgtg ctcattaatt aacagaacac
     1621 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat cacttcatca
     1681 tgaattcaaa ttcatcgatt aatatcaaca agatacaaaa agcactatca ttaaaattca
     1741 ttgcatttac attgatttca ccaatgaaat gtaaaaatat ataaacttga tgatttaagc
     1801 attttcttat aaccgttcag acgttattct tatttcagat catcgtcaga attgactcca
     1861 cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta tggtgttttg
     1921 atttatcttg cacctctcca cttctggata taaggatatt aggtatggca accgctggaa
     1981 tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat ctctaccttc
     2041 acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc cttcgcgccc
     2101 aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg
     2161 ctacccccat cgttaaagcc attgatgttc caggtgaaaa actgaactct ctggaagaac
     2221 tgttccaaaa aacgatggaa gaatacgagc aacgttcaag cacgctggcg cagttagccg
     2281 atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag
     2341 aacagcagca tgatggcctg ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac
     2401 ttgcgggtat gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg tcacaccagc
     2461 tgcattgatc atacatcggc gctaatacat tgcgccgatg aagtttttga gaaaccgctg
     2521 cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg taaacttgag
     2581 ttcaatcagc gcgatggctt tttggattgc ccgcatggtg accgggtctg cggcagcggg
     2641 atgatgagta aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg
     2701 cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag ctaccacatc
     2761 agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct gatagcattt
     2821 tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca ccgtcatcag
     2881 gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc ctggcggtac
     2941 gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat
     3001 cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat aatatgacaa
     3061 ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata
     3121 aaaatgataa tcaaaaaaca gcccccctat ttctgacacc tacagatggc aagaaatagc
     3181 gcctgccagg catcttttcc ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt
     3241 ctcttactct tctggctgga ccatgagact tctgattctg actctttcat taataacgct
     3301 ggcggggtgt acggttactc gccaggccca tgtgagcgaa gttgacgcgg caacaggtat
     3361 tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc gttatgtcag
     3421 tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcga tcccctttgg
     3481 tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata cggaattcac
     3541 tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat tttaatcagc
     3601 gaggggatct tcgctgatta aagaaatagc ggtaatgcgt tttattccca ttcgtatttt
     3661 taataattga agtttatatt ttaccttttg caaataataa aataacaaat tatagtgacg
     3721 ccacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa
     3781 aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc
     3841 gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag
     3901 gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg
     3961 cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag
     4021 gaaacctata aacacgaaca attaatcacc cagaaaatta acgaactggc tcatgctgca
     4081 atgaccaatc aggactaccc aacatttaat ttcctgcagt ggtatgtttc tgagcagcat
     4141 gaagaagaga aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc
     4201 gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct
     4261 ccggcggcag gtttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt
     4321 cgaaaaacct tcttctgaag gtataaattt gccttttgct gccagaaacg ctaccagctc
     4381 tcccgctgtc atcccttctg ccgagcacgt gtgaaaacgt gcctgttcac caaaacgcgc
     4441 tttaatcgca gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt
     4501 taacacttca tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc ctagcgtagc
     4561 gattaccgct tatgaagact ttgcgccagc gcaggactga atgcttttat tgtacattta
     4621 tatttacaca atatgtaacg tcggtttgac gaagcagccg ttatgcctta acctgcgccg
     4681 cagatatcac tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag aaccctggga
     4741 agtgttttta tcgtggcggg aaccacaatt ggcgcaggca tgctggcaat gccgctggct
     4801 gcggccggtg ttggttttag cgttacgtta atcttgttga ttgggctttg ggcgttgatg
     4861 tgctacacgg cgctattact gctggaggtg taccagcatg ttccggcaga taccggtctg
     4921 ggcacgctgg caaaacgcta tctgggacgc tacggtcaat ggctgacggg cttcagtatg
     4981 atgttcttaa tgtatgctct gactgcagca tacatcagcg gtgccggtga attgttggcc
     5041 tccagcatca gcgactggac aggtatttca atgtcggcaa ccgctggcgt gctgttgttc
     5101 acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc tggtcgattt atttaaccgt
     5161 tttctgttca gcgccaaaat tatttttctg gtggtgatgc tggtattgct gctaccgcat
     5221 attcacaaag tgaatctttt aaccctgccg ttgcaacagg ggctggcact gtctgcaatc
     5281 ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc cgagtattgt cagctatatg
     5341 gatggcaaca ttcgtaagct acgctgggtg tttataatcg gtagtgccat ccccctggtg
     5401 gcatatattt tctggcaggt ggcgacgctt ggcagcattg attcaacaac ctttatggga
     5461 ttgctggcta atcatgctgg attaaacggg ctgttacagg cgttacgcga aatggtgccc
     5521 tctccgcatg ttgagctggc ggtgcattta tttgctgatt tagccctcgc cacgtcattt
     5581 ctcggcgttg cgttaggctt atttgattat ctggctgatt tgtttcagcg ttcaaatacc
     5641 gttggtggac gattgcaaac tggtgcaatt acctttctgc cgccgttggc gtttgcactg
     5701 ttttatccac gaggatttgt gatggcgctg ggttacgccg gtgtggcgct ggcggtactg
     5761 gcattgatta tcccttcact attgacctgg caaagcagaa agcacaatcc tcaggcgggt
     5821 taccgggtca aaggtggtcg tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg
     5881 attggcgtgc aatttttgat tgcggcaggg ttgttaccag aagtggggtg atcagatagc
     5941 ctcaaattcc ttattgggtg ccagaattaa cgctgacacc caatgtgacc tcttaatgca
     6001 ggcagcactg cttaaatttc ttaccactgc cgcacggaca aggatcgtta cgccccggtt
     6061 tctcttctgc tttgatcggt tgctgaacag ctttttcctg cggatgcgcc atccagtatg
     6121 catgtaaatc aagcgccgcc agtcgaatgg catctacgct ctcttcaaac gcttctggcg
     6181 acattttctc tacccgctcg aagttttcct cagtaccgtg cagcgcaatc gcctccagcg
     6241 ctggttttaa agaatcgggc aacgttgacc agtcagaaag tgccacgccc cgcatatagc
     6301 caaagcacca ctcctcaaca atcgtcagct cgctgccatc aacttctcgc aagccgaata
     6361 acggctcaaa ctgctccggg aattcgttca gacgctctgc ggtatcggcc atatgttgaa
     6421 aagccaggtt cataaagcgc gtcatctctt tctctgacgc ccaacgcggc acatagtcag
     6481 ccccacccca cacggcaacc agccactgtt ctggttcaat ctcttgcgga gaactcaaca
     6541 ccgccgtcaa taaaccgtcc agctccgcca catcaaggat ggcgtggtca gtgttgtatt
     6601 tggtcagaat atcgtccagc cattccaact cactttcgtt taacggtccc gttttcatac
     6661 gcttttcctt gtggatctca actcgccagc acctatctta catgccggtc cgtatcagag
     6721 atactttttg agtggctttg ccggtgatta aaaattaagg agggtgtaac gacaagttgc
     6781 aggcacaaaa aaaccacccg aaggtggttt cacgacactg cttattactt tgattttatt
     6841 cttatctttc ccatggtacc cggagcggga cttgaacccg cacagcgcga acgccgaggg
     6901 attttaaatc ccttgtgtct accgattcca ccatccgggc tcgggaagaa agtggaggcg
     6961 cgttccggag tcgaaccgga ctagacggat ttgcaatccg ctacataacc gctttgttaa
     7021 cgcgccaaat tcttcaggcc tttcagccag acatccgctt gacgccgatg tcttttaaac
     7081 tggagcggga aacgagactc gaactcgaga ccccgacctt ggcaaggtca tgctctacca
     7141 actgagctat tcccgtattc atcaagcaat cagttaatca cttgatttta ttatcgtctg
     7201 gcaatcagtg ccgccgttcg atgcgttgca ttctacttac ctggcgcgat gagtcaacga
     7261 tatttttcac cacttttgat cgtttgctga aaattacgcc gaaacgatca ctgatcaagc
     7321 aaatctgcac gcgcagcgct caaatattgc aacattgacc acagagtcag taccgcagcc
     7381 acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt tcggacgcca cagcagccag
     7441 gccaacgcca ccatctgggc agtggttttc actttcccaa tccaggagac ggccacgcta
     7501 ctgcgtttac ccaactccgc catccattcg cgtagcgcag aaataataat ttcacgggcg
     7561 atcatcgttg ccgccggtaa tgtcacccac cagctgtgat aatgctcggt taccagcacc
     7621 atggcgatag ccacgagaac tttatctgcc acagggtcaa ggaaagcacc aaaccgggta
     7681 ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt cagtcaccgc cgcgacgcag
     7741 aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca gataaaagac caatacaaag
     7801 aatgggataa ggatgacacg gaacagtgta agcaacgtag ggatattaaa ttgcataatg
     7861 acgggtaact atctgttgtc agtaagatta cccctatgtt gctacagaga catcaatgtt
     7921 tcaacgacca gaagatcttt tctgccagac cttgcgaaat acccggcact tttgcaattt
     7981 cctcgacgct ggcgttacgt aaaccttgca aaccgcccat atatttcaac aacatttgcc
     8041 gacgttttgg cccgacgcct tcaatggttt ccagggaact ggtatttttg accttcgccc
     8101 gttttttacg gtgcccgcca atcgcgtgat catgtgattc atcgcgaata tgctggataa
     8161 catgcagcgc gggtgaatct ggcggcaaac taaatccctc accttccggc tcaaagaaca
     8221 gcgtttccag tccagcctta cgatctgctc ctttggcaac gccaagtagc agcggatgat
     8281 ttttatccca tgagacatcc agttcggcga agacattttt cgcctgcgca agctggcctt
     8341 tgccgccgtc gataaggatc acatccggga tcttactgtc gtcaatggct ttaccataac
     8401 gccgacgcag cacctgattc atcgccgcat aatcatcgcc cggcgtgatg cctgtaatgt
     8461 tatagcgccg atactccgca cgcagcgggc cgttagcatc aaacaccaca caggaagcga
     8521 cggtttgttc gcccatggta tggctgatgt caaagcactc catccgcttc acttccggca
     8581 atttcaacac gctggcaagc gcggtcagtc gctggtgaac ggtagattgc tgcgaaagtt
     8641 tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt cagataacgc gccctatcgc
     8701 cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag ttctgaaagg gaatcggcga
     8761 gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc acccggtaag gtgcgcatct
     8821 ggctgccttg taaatagaac tgacctacga aggtttctac cacctcgctc agttccgtac
     8881 cgccaggcac tttcgggaaa tagctgcggc tgccgagcac tttgccctga cgaatgaaca
     8941 atacgtggac acaagccatg cccgcatcga acgccacacc aataacgtca aggtcgtcgc
     9001 cggtattgga aacaaactgt ttttcggtga cgcgtcgcac cgcctgaatt tggtcgcgaa
     9061 tacatgccgc ttcttcaaac tccagattct ggctggcagt ttccatacgg ctaatgagtt
     9121 gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg cacatactcg acctgctgag
     9181 cgtattcttc ttcactcacc agtccttcaa cgcacggtcc cagacagcgc cctatctggt
     9241 attgcagaca cggacgcgag cgattgcgat aaacactatt ttcgcactgg cgaatgggga
     9301 aaatcttttg cagtagcgcc agtgtttcac gtacggcata gccattcggg aacgggccga
     9361 aatattcacc tttggcatgc tttgcaccac gatgcatcgc cagacgcggg tgggtatcgc
     9421 cactcaggaa gataaaagga taagatttat catcgcgtag caaaacgttg taacgcggct
     9481 gatagagttt gatgtagttg tgttccagca acagcgcttc ggtttctgta tgcgtaaccg
     9541 ttacatcaat ttgctggatc tgggcgacca gcgcttcggt tttgcgcgaa gcgaggttgc
     9601 tgcggaaata gctggaaagc cgttttttca ggtctttcgc tttgccgaca tagataaccg
     9661 taccaccagc atcgtacatg cgataaacgc ctggctggct ggttacggtt tttaaaaacg
     9721 cttttgcgtc aaactgatca ctcactgact tgataatgtc tccgcattac acagaccatg
     9781 gcgaattgcc aggtgagtca gctcaacatc gccatgaatg tttagtttac tgaacatacg
     9841 atagcggtag ctgttcaccg ttttcggact gagattgagc tgttctgaga tctcattgac
     9901 cttctggccc ttagtgatca tcagcataat ctgcaattca cgttcagaca aactggcaaa
     9961 tgggctttct gttttttctg gttcgatctg gcttaacgcc atttgttgag cgatatcaga
    10021 agcaatgtaa cgctgccctg aatagacaga acgaatcgca ctcacgactt cctgcggagc
    10081 cgcgcctttg ctgaggtagc cagcagcacc tgcctgcatg actttcgctg gtaaagggtt
    10141 ttctgtatgg accgtaagca tgatgatttt aacatcagct gtggaacgcg cgattttacg
    10201 cgtcgcctca agaccgccaa tgcccggcat actcatgtcc attagcacca cgtcaacggc
    10261 atttgtccgg caccacttaa cggcgtcttc accgcacgat gcctctccga cgacttttat
    10321 accctttata tcttccagaa tgcgtcgtat ccctgcgcgc accagttcgt ggtcatcaac
    10381 aagtagaacg ttgatcaaag gaatatctcc agaaataggg ataacgctac tgatagttag
    10441 tcattcgtat attagcggtt tttattgcaa ctttgaaacg ttaaaaatgg tgggtattcg
    10501 atttttctct cgtttttgtc aattcgattg tccacagtta tggtcacgcc cgtcaccaac
    10561 ggcttaccgg atcgtttcac agtaagccct acgatgaaat gtgacaaaaa ttgactttat
    10621 tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt aattaacccg aacaatacgg
    10681 gcaaaaaaca ttcatgataa aaatatttat tggtcattat attaacgtat tttatagcac
    10741 tgccgatatc acgctcaaaa aacaaccact gctattttta gcaaagctta tggtatactc
    10801 cgccgcctta acatttttca ccgcaaattt tcattgcaac atgacgagga aaataaatga
    10861 gtacgcctga tttttctact gccgagaata atcaagaact ggcaaatgaa gtctcctgcc
    10921 tgaaagcgat gctgacgctg atgctgcagg cgatgggaca agctgacgcg ggccgcgtga
    10981 tgttaaagat ggaaaaacag cttgcgctga tcgaagacga aacccaggct gcagtatttt
    11041 ccaaaacggt taagcaaatt aaacaagcct accgtcagta atataaaacc ggctgagagc
    11101 gtgcctttca gccggttatt gacaaaatca tcgctatctc tgctgatgtc ttcccttccg
    11161 ccgtccattt cagaatttct ttttcgcgct tgctgaaatt catctctggc gtcatcacta
    11221 tttcatcatt taaacgcatc agagccatca gactttcgcg caccagtaac tgcattttta
    11281 attgcagttc atcactaaga atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa
    11341 agcccagcgc ccgatttggc agcattaaat actgagtgac accgcggcgt aaaccatgtg
    11401 cgtgcgcggc ttcccataat ggctgtgctt cgctgaataa gtcatcattc cacattaaat
    11461 ggtcctgact aaagttttca gggttcagca caggatcaat tgcgagaaaa ttttttgcct
    11521 gataataact aacccacgac tcagggtaat tggtgtagaa agccacttta ggtcgcgtga
    11581 atggtaccgg gtggcgaaca cataacgaat agtaatcgta ctccagctgc tgagcctgaa
    11641 gctctatttc atggtagacc tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg
    11701 tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg
    11761 agtatcattt ataatgataa ttcaaactat cacataaata agatttatat ataatttata
    11821 ttattcaggc aatgaattac ttttgcaagc catcgcattc tcttatgtta ttaatgagtt
    11881 atgctgattt gttaagcagt tttatcacgc ttgaaatggc gtcccgcccc gacaggtgaa
    11941 tcgtcggggc tgattttttc ttattattgc agcagaagct tctcgaggaa ctggcgggtg
    12001 cgaggctgct ggggatcggc aaataacgct tttgctgccc cctgctcgac tatccgcccc
    12061 tggtccataa agatcgcccg gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca
    12121 atcaccatcg tgcgcttttc ctgcgccagc tggcggatgg tgttcaggac ttcgcccacc
    12181 agctctggat ccagcgccga cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc
    12241 agtgcacgcg caatcgcaac acgctgctgt tgacctccag acaaacgacg tggatagctg
    12301 gtttctttac cggccagccc aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct
    12361 tctttcggtt cgcctttgac gatcaccggc ccttcaataa tgttttccag caccgtacga
    12421 tgcggaaaca aattaaagtt ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc
    12481 agcgattttt gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc
    12541 gttcccgctt ccggttgttc cagcagattt atgctacgta gcaatgtggt tttgccggaa
    12601 ccactcggac cgataattgc caccacttcg ccaggcttta cctcgaggtc gataccgtgc
    12661 agcaccgtct gaccgtggaa ttttttcacc aggttcttaa cttcaatggc actcattttg
    12721 gttctctctc ctggcgatta agttgattct caaaatggtt ctgcaacgtc gataacacag
    12781 tcgccatgat ccagtagatg agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc
    12841 gcgaggtaat caactgcgcc tgacggaaca gctccggcac ctggattgtc gcggccagcg
    12901 acgtatcttt taccagactg ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg
    12961 cctgtggcaa aatcgcgcga cgcatggtct gccacggcgt cataccaata ctggcagcgg
    13021 cttcccactg acctttatca atagaagaaa tggcggcgcg cagcgtttcg gcggcatagg
    13081 cggcagtgtt cagcgacaga ccaatcattg ctgacggaat gggatctaat tcgataccaa
    13141 actgcggcag gccgtaatag atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa
    13201 tagagatata aaaacgcgcc agccagcgca cgggccagat tggcgacagg cgcatcagcg
    13261 cgagaataaa cccgagtagt aagccaaaaa acatgccgcc gatactgagt tgcagggtat
    13321 acccagcccc tttcaacagg aacggcaagg aatcaataac cagttgtata ctttcttgca
    13381 tgagcgttcc cggagttaaa cgtggggatg ataggcgaac agcgcaggcg cgccgccggt
    13441 atgaataaac agaatcggcc cttcatcttt gaagcgtttc tgactgatac cgtcaatcag
    13501 ccccgccatc gcttttccgg tatacacagg atcaagcaga ataccttcca gacgcgccag
    13561 caatttcact gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc
    13621 atcccagagt aaaatttccg ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg
    13681 taggttaacc actttcggca attgatcggc aacggaacgc gacacggtca cgccaatcag
    13741 ttcgctttca ggcatcaggt gttccagccc aacagccagt ccggcgtgag ttccggcact
    13801 gcccgatgcg actaccaccg acgaaatatt aaccgcccct tcacactgtt gcgcgatttc
    13861 cagcgcactc tccacataac ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac
    13921 atacgggcga aagccttgtg cttcgactcg cgtcgccagc tcttccagtt gggcattggg
    13981 atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac agatccagca acaaacgatt
    14041 gccgttggtt aaatagtttt ctgcggttgt gccaatagga ttttccagca gcgccacgca
    14101 gtgcagaccg agtttcgccg cgactgcggc agtctggcgc acatggttag actggatcgc
    14161 gccggcagta atcagcgtat cggcaccttc gcgcagggca tctgccgcga gaaattccag
    14221 cttacgtaat ttattgccgc ccattgccat gggggtgacc tcatcccgtt tgatgaaaat
    14281 ttcccgtcca agataatcag aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat
    14341 aaactccaac cgtggaaaac gggttaaatt atgcagtggc ataacagcct ccgatgtgtg
    14401 ttgttgtgat tttcttatta tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa
    14461 agcgcccttt tttgtcatta tgctgattat ttggtcacat cagcaccaaa ccatttttcg
    14521 gaaagagctt gcagagtgcc gtctttttgc atttccgcaa ttgcatcatt caccgctttc
    14581 agcaggtctt catttccttt acgcagcgcc acgccagact cctgacggga gaatgcttca
    14641 ccggttactg ccagcgtatc gttggttttc ttcaccagat ccagcgccgc cagacgatca
    14701 acgaggatcg catcgatacg ccctacgcgc agatcctgat atttggtcgg gtcatcatca
    14761 taggtacgca catcgacacc ctgaacattc tgccgcagcc actcttcata gttggtgccc
    14821 agaccgacac ccactttttt gcctttcaga tcagcagccg ttttaatggt gccttcgtta
    14881 ccttttttca ccagcgcctg aataccggaa atggtgtaag gggtagagaa atcgtatttt
    14941 ttcttgcgct cgtcagaaat ggtgacctga ttaatcacca catcaatgcg tttagagtcc
    15001 agcgacgcca gcataccgtc ccatttggtc ggttttagtg acgcctcaac gccaagatgt
    15061 tttgccagct gttgggcaaa ttccacttca aaaccggtta atttgccgtc atccccctga
    15121 aaactgaacg gcggataagt gccttccagc cctaccagca gcgtgccgcg ctctttgact
    15181 ttattaagca gaccttcatc tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg
    15241 cccatcacac ccatcaatgc ctgacgtccc agatgtgcta atttcatatt caccccgaat
    15301 gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac aactccgcta
    15361 catcttattc ttatttaata tatatcagaa gcaggcaggc tggaggattt ctgcacaagt
    15421 ccggtacagg tttggcgctg atagtgttga tacttccgta atgcgatgcg gtaattgttg
    15481 gtgctggtgg tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga
    15541 tcgagggaaa tattttgctc atgtagatga ttacccaaac ggcgcagacg aacgacatat
    15601 tcacgcaccg tacctgggct catttcagtt tgctcaaaaa gaaatttctt aaagccgata
    15661 atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc
    15721 gcccatgatt gttgttccgt ttgccagcct ttttcatcaa gcagcgtgtc caggcgggaa
    15781 atctcgattt tattcactat tttgccgtcg cgaactaagg taatgcgatc gagtaattta
    15841 cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg gcttaatggc
    15901 cgtcttttca ggtgctgcac catcattaag aactcctggt agtcaaagtt aaagtgcggc
    15961 atttactgac gttataactt acccagttta gtgcgtaacc gtttaatggc ctggctgtgt
    16021 aactgactga cccgcgattc cccgacctcc agcaccgcgc caatctcttt gagattcagc
    16081 tcttcctggt aatagagggt taataccagt ttttcgcgct ccggcaacgt ttcgatggct
    16141 tccatcaccc gctggcgcag attactgtcc agcagttgtt gtagcgggtt ttctcgctga
    16201 tgatcatcag taaccagttc gatgctatcg ccgtgctctt cacgccactc atcgtaggag
    16261 aagagctggc tgttattggt gtcgagcagc atttgacgat aatcggcaat atcgatccct
    16321 aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc cagttgccct
    16381 attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg
    16441 cgaagttcat ccagcatagc gccacggata cgctgcactg cgtaagttgt aaatgccgtt
    16501 ccttgtgggg cgtcatagcg ttcgacagca ttaagtaacc caatgccgcc cgcctgtagc
    16561 agatcgtcaa gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc
    16621 accagcggga cataacgctg ccacagcgag tgtttatcca ttacaccttc agcggtatag
    16681 agtgaattca cgataaacag ccctgcgtta tatgagttat cggcatgatt atccgtttct
    16741 gcagggtttt taatcggacg attagtgggt gaaatgaggg gttatttggg ggttacgcaa
    16801 aaagccgaat gtaaaaaacc ccgtcggtgg cggggtttga gaggcattca gacgattaac
    16861 cctgcagcag agacagaacc tgctgcggta cctggttggc ttttgccagc acggagttac
    16921 cggcctgcag gataatctgc gctttcgaca tgttggacac ttcggtcgca tagtcggcgt
    16981 cctgaatacg ggactgcgct tcagacaggt tggtagtggt gttgttcagg ttggtgactg
    17041 cggaatccaa acggttttgc accgcaccca gggaggagcg gaatttgtcg atggagctga
    17101 tggcttcatc cagagctttc aggggatcgg ctgtcgtagc tgcttttgtt tcagcatctg
    17161 tcgtcaattt accatcagca tctttataaa ctgccttacc gctgccgtta gtcactgaac
    17221 catcatcctg taggtaaaaa tctacagtat cagatgtggt caacgcctcg ttgttatcaa
    17281 cataaaccga atcaacacct tttgttacag cgccagttgt gccgttgacg ctataacctg
    17341 cagtagtgaa atcaacattg tttcccgagg ttgatacagc tttatcgaaa gctgcctgat
    17401 caacttttgc acctgttact gaataagtaa tagtgtccgg cgtagtacta ttaccggtaa
    17461 aggagataga ggtaccgcca atgtcaatgg ttgtcgcttt ggtattattg gctgcaagtg
    17521 tggacagact agcctcagtc aaagaagcac cagagccgtt tttcgttaag cgcccagttg
    17581 tatcaatgta gagtttatct ccattgcttg ccgtaatttt accatcactg ctgatctgaa
    17641 catcctgtgc agaaccatcg atagtaatag tgcccttagc ggtatcacca actcccgggg
    17701 tcaaatattt ctgaacatcg gcagctgaag ccgttgtggt atcaaaagag tatgacttac
    17761 ttgcactgtc atacttgtaa ttcgttgcac tagccgccgt tccgaagccg ttattaatgg
    17821 ttgcctgaac agtatcacca tcactaactc cagccagcaa atcagacgct ttagcagcat
    17881 cgtaacccgc actcacagta tatttgttgc cgaccactgt tgcattagca gctaccaagt
    17941 cagctttaga tgcagcagta ttagccacag ccccgccacc attcacatta aacccattca
    18001 gccccagcgt atcagagtca attttcttca gatcaatagt gatagtctgg ccgtcattcg
    18061 caccaacctg aattttcatc gaaccgtctt ttgccagtac gttcacgccg ttgaactggg
    18121 tctgaccgga tacgcggtca atttcgtcga gacgggattt gatttcgtcc tgaatggagt
    18181 ccagatccga atcagagtta gtcccggtag aagcctgaac cgtcagctca cggatacgct
    18241 gtaagttgtt gttgatttcg gacagcgcgc cttcagtggt ctgtgcaaca gaaataccgt
    18301 cgttggcgtt acgtgcagcc tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa
    18361 tcgcctgacc cgcggcgtca tccttcgcgc tgttaatacg caagccagaa gacagacgct
    18421 cgatagaact cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga
    18481 ggctgttggt attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt
    18541 acgttttggg tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa
    18601 aacaaccttt agaatttttt tcacaaacag ccattttttg ttagtcgacg aaatactctt
    18661 ttctctgccc cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc
    18721 aggccgataa ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa
    18781 gtatttcatc gctgggagtc gggtcaggtc tggatttaag ttcaatcctt gatagcctca
    18841 ccgccgcgca aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta
    18901 aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac tttccagacc gccaatactg
    18961 cattgtctaa agccgatctt ttttccgcca ccagcaccac cagcagcacc accgcgttca
    19021 gtgccaccac cgcgggtaac gccatcgccg ggaaatacac catcagcgtc acccatctgg
    19081 cgcaggcgca aaccctgacc acgcgcacca ccagagatga tacgaaaacg gcgatcgcca
    19141 ccagcgacag caaactcacc attcaacaag gcggcgacaa agatccgatt accattgata
    19201 tcagcgcggc taactcatcg ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg
    19261 gcgtaagtgc gagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa
    19321 atgacaccgg ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa
    19381 gttttatggg ctatgacgcc agtgccagca gcaacggtat ggaggtctcg gtcgccgccc
    19441 agaatgcgca gctgacggtc aacaacgtcg ccatcgagaa cagcagcaac actatcagcg
    19501 acgcgctgga aaacatcacc ctgaacctga acgatgtcac cacgggcaac cagacgctaa
    19561 ccatcactca ggacacctcc aaagcgcaaa cggcgattaa agactgggtg aatgcctata
    19621 actcgctaat agataccttc agcagcctga ccaaatacac cgccgtagat gcgggagctg
    19681 atagccagag ttctagcaat ggcgcactgc tcggcgactc cacgctgcgg acgattcaga
    19741 cgcagttgaa gtcgatgctg agtaataccg tcagttcttc cagctataaa acgctggcgc
    19801 agattggtat cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca
    19861 ccgctgcact gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa
    19921 aaaccggcat cacaaccacc atcggcagca acctgaccag ttggctttcg acaacgggca
    19981 ttattaaagc cgctaccgat ggcgttagta aaaccctgaa taagttaact aaagactaca
    20041 acgccgccag cgatcgcatt gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac
    20101 tggacgtttt aatgacctcg ttaaacagca ccagcagcta cttaacgcag cagttcgaaa
    20161 acaacagtaa ttccaagtaa tcattattca tcgggagaca ggtcatgtac gcggcaaaag
    20221 gcacccaggc ctatgcgcaa attggcgtcg aaagcaccgt aatgagcgcc agccaacagc
    20281 agttggtcac catgctattt gatggagtgc tgagcgcact ggttagagcg cgactgttta
    20341 tgcaggacaa caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg
    20401 agaacggact gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttaa
    20461 ttgctcttta tagctatatg gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct
    20521 ccgcagtcga agaggtggaa gcattaatgc gtaatattgc cgatgcctgg aaagagtctt
    20581 tactctcccc ttcttcgatt caggacccag tctgatgaac aatgcaccgc atttgtattt
    20641 cgcctggcaa caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca
    20701 atgggatgaa ctcatcgcta gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca
    20761 cctgactgaa gaggttgccc cgtccactac gatgcaggag cagctccgcc cgatgctgca
    20821 cctgattctc gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact
    20881 ggcgaaactg gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggcgatca
    20941 gggcggcttt gtgctggctc cgcaggataa cctcttttga atcttaatga gccgatggct
    21001 cgcgaataat ccgattacgg ctacgcttct aatattcccc ttgaatggag tcgaagaatg
    21061 cgtaatccca cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg
    21121 cctgaactgg ccgagcgcgc agacggtttt aatgatattg gtatcaatat ggtctggttg
    21181 ccgcccgcct ataaaggcgc atcgggcggc tattcggtcg gctacgactc ctatgattta
    21241 tttgatctag gtgagtttga tcagaaaggc agcatcccta ctaaatatgg tgataaagca
    21301 caactgctgg ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg
    21361 gtagtcaacc acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat
    21421 gctgatgacc gtacgcaaat tgacgaagaa atcattgagt gtgaaggctg gacgcgttac
    21481 accttccccg ctcgtgccgg gcaatactcg cagtttatct gggatttcaa atgttttagc
    21541 ggtatcgacc atatcgaaca ccctgatgaa gatggcattt ttaaaattgt taacgactat
    21601 accggagaag gctggaacga tcaggttgat gatgaactcg gtaatttcga ttatctgatg
    21661 ggtgagaata tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc
    21721 tgggtgatgg aacaaacgca atgcgacggc tttcgtcttg atgcggtcaa acatattccg
    21781 gcatggtttt ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt
    21841 attgtggctg aatactggtc gcatgaagtc gataaactgc aaacgtatat tgatcaggtg
    21901 gaaggcaaaa ccatgctgtt tgatgcgccg ctgcagatga aattccatga agcatcgcgc
    21961 atgggtcgcg actacgacat gacgcagatc ttcactggga cgctggtgga agccgatcct
    22021 tttcacgccg taaccctcgt tgccaaccac gacacccaac cgttgcaagc cctcgaagca
    22081 ccggtcgaac cgtggtttaa accgctggcg tatgccttaa ttttgttgcg ggaaaatggc
    22141 gttccttcgg tgttctatcc ggacctctac ggtgcacatt acgaagatgt cggtggtgac
    22201 gggcaaacct atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc
    22261 cgtcagcgtt tcgcccacgg tgtacagaca ttatttttcg accatccgaa ctgcattgcc
    22321 tttagccgca gtggcaccga cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat
    22381 gatggcgaaa aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt
    22441 ctggggaacc ggcaagagag cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc
    22501 aacggcggca gcgtcagcgt gtgggttatc gaagaggtga tttaaatcca tccccggcgg
    22561 caagccgggg agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc
    22621 ggcacactct gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt
    22681 gttaccgtca cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc
    22741 agacatcaat gtcaacttac ccggcacggc aatcccccgc tgccactgac ggcaatccaa
    22801 ggtatcgccc tctttagtga caatcaggct gcctatcgct tccggactga ccagcgcacg
    22861 ttgcggccct ttggtctgcc agtagcccgc cagccagtcc ggcgcggggg ttttcacgac
    22921 attgttatag ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc
    22981 gagttttttc atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagctctg
    23041 atgtcgccag cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt
    23101 attaccccga aaataaattt gcatggtgta tgattcgcgg ctcttgtttc aaaccttctg
    23161 agttcagagg ctacattcat gtcatggcag caattcaaac acgcctggtt gattaaattc
    23221 tgggcaccca tccctgcggt catcgcggcg ggtattctct ccacttacta ttttggcatt
    23281 actggcacct tttgggctgt cacgggtgaa tttacccgtt ggggcggtca gctcctgcag
    23341 ctgtttggcg tccatgctga agagtggggt tattttaaaa ttatccatct ggaaggatcg
    23401 ccattaaccc gcatcgacgg catgatgatc ctcggtatgt ttggcggctg ctttgctgct
    23461 gcgctgtggg ccaacaatgt caaactacgc atgccacgca gccgtatccg cattatgcag
    23521 gccatcattg gcggcattat cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg
    23581 gcggcgttct ttaccggcat tcctcagttc tcgctgcatg cctggttctt tgccatcgcc
    23641 actgccattg gttcatggtt tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc
    23701 gtgaaaatgc agaaagtttc tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg
    23761 cgtcgttttc gtctcgggat gctggtcttt ttcggcatgc tgggctgggc gctgctcacg
    23821 gcgatgaacc aaccaaaact ggggctggca atgctgtttg gcgtcggctt tggtttactg
    23881 attgaacgtg cgcaaatctg ctttacttcg gcgttccgcg atatgtggat caccggacgt
    23941 acccatatgg cgaaagcaat cattatcggt atggcggtga gtgccatcgg gatcttcagt
    24001 tacgtacagt taggcgttga acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt
    24061 ggtttacttt ttggttttgg catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac
    24121 cgcgcggtag aaggccaggt gcactactgg tgggtcggtc tgggcaacgt gatcggctca
    24181 acgattctgg cgtattactg ggatgatttc gctccggcgc tggccaccga ctgggacaaa
    24241 atcaacctgc tgaaaacttt tggtccgatg ggaggcctgc tggtgacata tttgctgctg
    24301 tttgctgcgc tgatgttgat tatcggctgg gaaaaacgct tcttccgccg tgcggcaccg
    24361 cagactgcta aggagatcgc atgaaaaata tcgttcctga ttaccgcctt gatatggtgg
    24421 gtgaaccctg cccttatccg gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag
    24481 gtgagatcct ggaagtggtg agcgactgtc cgcagtcgat caataatatt ccactggatg
    24541 cgcgtaatca cggctacact gtgctggata ttcagcaaga cgggccgacc attcgttatt
    24601 taattcagaa gtaatccctc attcccgctg tggcccacag cgggaacatc gttacctctg
    24661 acacatagat tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggttgct
    24721 atgtgtggac gctgtgccca atcccaaacg cgtgaggatt accttgcgct tctcgcggaa
    24781 gatattgaac gcgatattcc ctacgatccc gaacccattg gcagatacaa tgtcgcgccg
    24841 ggaaccaaag ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc
    24901 tggggatatg ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact
    24961 gcctcaacca gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc
    25021 gatggctggt ttgagtggaa aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg
    25081 ctgacggaca acctgttttt atggccgcga taggtagcat accgtttgag cgtggtgacg
    25141 aagccgaagg atttttgatc gtcactgcgg cggcagatca aggtctggta gatattcatg
    25201 accgccgccc gctggttctg tcgccagaag ctgcacggga atggatgcgg caagacattg
    25261 gtggtaaaga agcctcagaa atcgctacca gaagctgtgt gccagcaaac cagttcacct
    25321 ggcaccctgt gtcgcgcacg gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac
    25381 ctgtttgttg acatcctcca cgccctgaat gacgaggccc cctgctacgt tcaggctgtc
    25441 gcctgaatca tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca
    25501 caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccatttac atcggcgttc
    25561 gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcgatt ttctttcgct
    25621 gtatgaccac agcacgcgca acgctggctt gtgtacgctg gcggaacagc aagcacctga
    25681 ccgccacgcc agagctgctt gtactcaagc tggcggcgca tttcatacca gccctgatcc
    25741 agtatcgaac ggtttaaacc tgattttgcc cggacattgc gccccggctg gctgaccgta
    25801 cccgctgctg actttgacat gttgctgacc ttcaaatcct caatgactat cattacgtgg
    25861 tttttgctga cggtcgttgt gactttgtga aggtagtccc tgcggatatt tgcgatacag
    25921 gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt tgttgctgaa tttgaccttg
    25981 cggcttaact gtcgctgaag tctcgccagc ttcttctggt ttttctgaaa actgtttaca
    26041 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
    26101 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
    26161 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
    26221 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgatcg
    26281 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
    26341 aacgccgacc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
    26401 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
    26461 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
    26521 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
    26581 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
    26641 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
    26701 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
    26761 gacgcgacag aaaaactacg tacttacttt tcaaatgtat gtgctgattt tgaagctgaa
    26821 ctggttgaaa tggatggcga accagagcac gtccatctgt taattaacta tccccccaaa
    26881 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
    26941 acgggatcgg ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
    27001 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
    27061 acagcaaaca cctggtcagg tgaaaaaacg agccttatat ccccgccctg aaggacgggc
    27121 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact
    27181 atgccccgta catgccccat cacatcctca acatcaaagt attagagcct tgcaaaccat
    27241 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
    27301 cgctatattg cgtattaaat aaaaccacct caattacatt aataaatcta aataaatatc
    27361 atgcaaataa tttccatggt tatttttagg ctatttaaat taaaaaaata aattatgagt
    27421 caatcataaa taacaacctt taatataatt tgactaccaa atttatattc gttcacaaaa
    27481 tagtcatgca ttgtgtgcaa tagaaacagt tcagataaaa atagggatct acttcacaat
    27541 ttaaatgaga aactaaaact tacatcttga aataatctca ttgattggat gaatatttgt
    27601 agtgcagtga catcattttt aaataatagt tcaaataaaa gggctcacga tgaaaaaatt
    27661 aacagtggca atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc
    27721 agagatttac aataaagaca gtaacaagct ggatctgtac ggaaaagtta atgctaagca
    27781 ctacttctct tctaacgatg cagatgatgg cgacacaact tatgttcgtc tgggcttcaa
    27841 aggcgaaact caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa
    27901 aggcaaccgt gctgaatctc aaggttcttc caaagataaa acccgtcttg ccttcgctgg
    27961 cctgaaattc ggtgactacg gctccatcga ttatggccgt aactacggtg tagcatacga
    28021 tatcggtgca tggaccgacg ttctgccaga attcggtggc gatacctgga cccaaacaga
    28081 tgtgttcatg actggtcgca ccacaggtgt tgcaacctat cgtaacaacg acttctttgg
    28141 tctggttgat ggtctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt
    28201 aactgaagct aacggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg
    28261 tgtaggtgca acctatgcta aatctgaccg cactaatgat caggttatct acggtaacaa
    28321 cagcctgaat gcatctggtc aaaatgctga agtatgggca gctggtctga aatatgatgc
    28381 gaacaacatc tatctggcta ccacctattc tgaaacccag aacatgactg tttttggtaa
    28441 taaccatatt gccaacaaag cacaaaactt cgaagtagtt gcacaatatc agttcgactt
    28501 cggtctgcgt ccgtccgttg cttacctgca atctaaagga aaagacttgg gtgcgtgggg
    28561 tgatcaggac ctgattgaat atattgatgt aggtgcaacc tattacttca acaaaaatat
    28621 gtccactttt gttgattaca aaatcaacct gattgataag agcgatttca cgaaagcatc
    28681 tggcgttgct accgatgata tcgttgctgt aggattggtt taccagttct aatctgatta
    28741 ctcaaagata tgttgtggga ggctttgcct ccccaacata taagtggcgc cctcaagcca
    28801 ctttccttag aagcactatc ttgcttctga ctatataaat taccctttat tttctgggac
    28861 gtttcaacgc cccattttta ataatactca gtgaacaatt aacgtatcaa ttagatttaa
    28921 taacaacgat atccgtacct aacctgatat ctaatgtcat aagcatccct ttaattatcc
    28981 cctcaacctc ttgtaaattt cccactcttg agctccacat agtccaaacg ccggatgcaa
    29041 aaaaaatcac acgtgcatct ctgaaattgt gatggaggaa gtgagtatca attatatgtg
    29101 cttataaacg ttcccgattg gcacagaagt caccggattg ttcaggctcc aataggttga
    29161 gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt tgctccttaa taaaatactt
    29221 ttactctctg gacgcttttc ttagctctct tggtgttatc ttaaaacgat tatggaacct
    29281 ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt
    29341 tactgtcgtt tgtatagcag acaacgcatt atttaacctc acgtcgtcca atatactttg
    29401 aaaacttatt ccctcgct
//
LOCUS       DADLZM010000038        29082 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLZM010000038 DADLZM010000000
VERSION     DADLZM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.038"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="JNY73_001653"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="JNY73_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY8047694.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="JNY73_001654"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="JNY73_001654"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY8047695.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JNY73_001655"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JNY73_001655"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY8047696.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JNY73_001656"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JNY73_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY8047697.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JNY73_001657"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JNY73_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY8047698.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /locus_tag="JNY73_001658"
                     /pseudo
     CDS             complement(3421..4089)
                     /locus_tag="JNY73_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="JNY73_001659"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="JNY73_001659"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY8047699.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="JNY73_001660"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="JNY73_001660"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY8047700.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="JNY73_001661"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="JNY73_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY8047701.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JNY73_001662"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JNY73_001662"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY8047702.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JNY73_001663"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JNY73_001663"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY8047703.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="JNY73_001664"
     CDS             8207..9070
                     /locus_tag="JNY73_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY8047704.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="JNY73_001665"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="JNY73_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY8047705.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="JNY73_001666"
     CDS             10406..11023
                     /locus_tag="JNY73_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY8047706.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="JNY73_001667"
     CDS             complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="JNY73_001667"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY8047707.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12960..13583
                     /gene="gmk"
                     /locus_tag="JNY73_001668"
     CDS             12960..13583
                     /gene="gmk"
                     /locus_tag="JNY73_001668"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY8047708.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13638..13913
                     /gene="rpoZ"
                     /locus_tag="JNY73_001669"
     CDS             13638..13913
                     /gene="rpoZ"
                     /locus_tag="JNY73_001669"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY8047709.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13932..16040
                     /gene="spoT"
                     /locus_tag="JNY73_001670"
     CDS             13932..16040
                     /gene="spoT"
                     /locus_tag="JNY73_001670"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY8047710.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16047..16736
                     /gene="trmH"
                     /locus_tag="JNY73_001671"
     CDS             16047..16736
                     /gene="trmH"
                     /locus_tag="JNY73_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY8047711.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16742..18823
                     /gene="recG"
                     /locus_tag="JNY73_001672"
     CDS             16742..18823
                     /gene="recG"
                     /locus_tag="JNY73_001672"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY8047712.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="JNY73_001673"
     CDS             complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="JNY73_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY8047713.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLRLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20342..21733
                     /gene="xanP"
                     /locus_tag="JNY73_001674"
     CDS             20342..21733
                     /gene="xanP"
                     /locus_tag="JNY73_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY8047714.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21854..23563
                     /locus_tag="JNY73_001675"
     CDS             21854..23563
                     /locus_tag="JNY73_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY8047715.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNREWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="JNY73_001676"
     CDS             complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="JNY73_001676"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY8047716.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25944..27327)
                     /locus_tag="JNY73_001677"
                     /pseudo
     CDS             complement(25944..27327)
                     /locus_tag="JNY73_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27620..27714
                     /locus_tag="JNY73_001678"
     tRNA            27620..27714
                     /locus_tag="JNY73_001678"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27654..27656,aa:Sec,seq:tca)
     gene            27986..29082
                     /locus_tag="JNY73_001679"
                     /pseudo
     CDS             27986..29082
                     /locus_tag="JNY73_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         7182 a         7794 c         7310 g         6796 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccggaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacgcgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gccgctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaactt gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gccccagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccggcaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 gttggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cgctacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccttccagt agtcatcgtc ccactgtttt atttgctgtt gcaggcggga
    12601 aatttcttcc tgtgctctgg ctggcgacca ggccggacag accgcccaca cagatgattg
    12661 ccagcacaag atacttatta atatcgccat ccatactttc atcttcacct ccgctgtagt
    12721 caggctggca gatatacaac gtggtgaagg caaagccgag tggcaaaaac ggagtctgcg
    12781 aggacgcttc ctgagaatcg tctttattgc agtaaatcac aggcaaatgc ggaaacagct
    12841 acgcaaaacg caacaacttt gcgcaaaaag tgtgagcaag ggctacgtca catggccgcg
    12901 ccgtgtataa taagctcgta tgtaggcttt atttcgctaa tcacatacga aagatactca
    12961 tggctcaagg cacgctttat attgtttctg cccccagtgg cgcgggtaaa tccagcctga
    13021 ttcaggcttt attaaaaacc caaccgttgt atgacaccca ggtttctgtt tcacacacca
    13081 cacgccaacc gcgtcctggt gaagtccacg gtgaacatta tttctttgtt aatcatgatg
    13141 aatttaaaga aatgattagc agagatgcgt tcctcgaaca cgcagaagtt tttggtaatt
    13201 actatggcac ttcgcgtgag gccattgagc aagtactggc gaccggtgtc gatgtttttc
    13261 tcgatatcga ctggcagggc gcgcagcaaa ttcgccagaa gatgccgcac gcgcggagta
    13321 tctttatttt accgccgtcc aaaattgaac tggaccgccg tctacgcggt cgcggtcagg
    13381 acagcgaaga ggtcattgca aagcgtatgg cgcaagctgt tgcagaaatg agccattacg
    13441 ccgaatatga ttatctgatt gtgaatgatg acttcgatac cgcgttgacc gatttgaaga
    13501 ccattattcg cgccgaacgt ctgcgcatga gccgccaaaa gcagcgtcat gacgctttaa
    13561 tcagcaaatt gttggcagac tgaacctgat ttcagtatca tgcccagtca tttcttcacc
    13621 tgtggagctt tttaagtatg gcacgcgtaa ctgttcagga cgctgtagag aaaattggta
    13681 accgttttga cctggtactg gtcgccgcgc gtcgcgctcg tcagatgcag gtaggcggaa
    13741 aggatccgct ggtaccggaa gaaaacgata aaaccactgt aatcgcgctg cgcgaaatcg
    13801 aagaaggtct gatcaacaac cagatcctcg acgttcgcga acgccaggaa cagcaagagc
    13861 aggaagccgc tgaattacaa gccgttaccg ctattgctga aggtcgtcgt taatcacaaa
    13921 gcgggtcgcc cttgtatctg tttgaaagcc tgaatcaact gattcaaacc tacctgccgg
    13981 aagaccaaat caagcgtctg cggcaggcgt atctcgttgc acgtgatgct cacgaggggc
    14041 aaacacgttc aagcggtgaa ccctatatca cgcacccggt agcggttgcc tgcattctgg
    14101 ccgagatgaa actcgactat gaaacgctga tggcggcgct gctgcatgac gtgattgaag
    14161 atactcccgc cacctaccag gatatggaac agctttttgg taaaagcgtc gccgagctgg
    14221 tagagggggt gtcgaaactt gataaactca agttccgcga taagaaagag gcgcaggccg
    14281 aaaactttcg caagatgatt atggcgatgg tgcaggatat ccgcgtcatc ctcatcaaac
    14341 ttgccgaccg tacccacaac atgcgcacgc tgggctcact tcgcccggac aaacgtcgcc
    14401 gcatcgcccg tgaaactctc gaaatttaca gcccgctggc gcaccgttta ggtatccacc
    14461 acattaaaac cgaactcgaa gagctgggtt ttgaggcgct gtatcccaac cgttatcgcg
    14521 taatcaaaga agtggtgaaa gccgcgcgcg gcaaccgtaa agagatgatc caaaaaatcc
    14581 tctcggaaat cgaagggcgt ttgcaggaag cgggaattcc ctgccgcgtc agtggtcgcg
    14641 agaagcatct ttattcgatt tactgcaaaa tggtgctcaa agagcagcgt tttcactcaa
    14701 tcatggacat ctacgctttc cgcgtgatcg tcaatgattc cgacacctgt tatcgcgtgc
    14761 tcgggcaaat gcatagcctg tacaagccgc gtccgggtcg cgtgaaagac tacatcgcca
    14821 ttccaaaagc gaacggctat cagtctttgc acacctcgat gatcggcccg cacggtgtgc
    14881 cggttgaggt ccagatccgt accgaagata tggatcagat ggcggagatg ggtgttgccg
    14941 cgcactgggc ttataaagag cacggcgaaa ccagcaccac cgcgcaaatc cgcgcccagc
    15001 gctggatgca aagcctgctg gagctgcaac agagcgccgg tagttcgttt gaatttatcg
    15061 agagcgttaa atccgatctc ttcccggatg agatttacgt tttcacaccg gaagggcgaa
    15121 ttgtcgagct gcctgccggt gcaacgcccg tcgacttcgc ttatgcagtg cataccgata
    15181 tcggtcatgc ctgcgtgggc gcacgcgttg accgccagcc ttacccgctg tcgcagccgc
    15241 ttaccagcgg tcaaaccgtt gaaatcatta ccgcaccggg cgctcgcccg aatgccgcgt
    15301 ggctgaactt tgtcgttagc tcaaaagcgc gcgccaaaat tcgtcagttg ctgaaaaacc
    15361 tcaagcgtga tgattctgta agcctgggcc gtcgtctgct caaccatgct ttgggtggta
    15421 gccgtaagct gaatgaaatc ccgcaggaaa atattcagcg cgagctggat cgcatgaagc
    15481 tggcaacgct tgacgatctg ctggcagaaa tcggacttgg taacgcaatg agcgtggtgg
    15541 tcgcgaaaaa tctgcaacat ggggacgcct ccattccacc ggcaacccaa agccacggac
    15601 atctgcccat taaaggtgcc gatggcgtgc tgatcacctt tgcgaaatgc tgccgcccta
    15661 ttcctggcga cccgattatc gcccacgtca gccccggtaa aggtctggtg atccaccatg
    15721 aatcctgccg taatatccgt ggctaccaga aagagccaga gaagtttatg gctgtggaat
    15781 gggataaaga gacggcgcag gagttcatca ccgaaatcaa ggtggagatg ttcaatcatc
    15841 agggcgcgct ggcaaacctg acggcggcaa ttaacaccac gacttcgaat attcaaagtt
    15901 tgaatacgga agagaaagat ggtcgcgtct acagcgcctt tattcgtctg accgctcgtg
    15961 accgtgtgca tctggcgaat atcatgcgca aaatccgcgt gatgccagac gtgattaaag
    16021 tcacccgaaa ccgaaattaa tgttttatga acccaacacg ttatgcacgc atctgcgaaa
    16081 tgctcgccag gcggcagcct gatctgaccg tctgcatgga gcaggtccac aaacctcata
    16141 acgtttctgc gattattcgt accgcagatg ccgttggcgt acatgaagtt cacgccgtct
    16201 ggcctggtag ccgcatgcgc accatggctt cggcagcggc gggtagtaac agctgggtac
    16261 aggtgaaaac acaccgcacc attggcgatg ccgtcgctca tctcaaaggc cagggcatgc
    16321 agattctggc aacccatctt tctgataatg ctgtcgattt ccgcgaaatt gattacactc
    16381 gcccgacctg cattttgatg ggacaggaga aaacgggcat cacgcaggaa gcattggccc
    16441 tggcggatca ggacatcatc attccgatga tcggcatggt gcagtcgctg aatgtttccg
    16501 ttgcctcagc cctcattctt tacgaagccc agcgtcagcg gcaaaatgca ggcatgtacc
    16561 tgcgtgaaaa cagcatgttg ccggaagcag agcaacaacg cctgttgttt gaaggcggct
    16621 atccggtgct ggcgaaagtc gcaaaacgca aaggcctgcc ttatccccac gtcaatcagc
    16681 aaggcgagat cgaagctgat gccgactggt gggctactat gcaggctgca gggtaagtgc
    16741 catgaaaggt cgcctgttag atgctgtccc actcagttcc ctaacgggcg ttggcgcagc
    16801 acttagtaac aaactggcga aaatcaacct gcataccgtg caggatctac tcttacacct
    16861 tcccctgcgc tacgaagatc gcacccatct ctaccccatc ggagaactac tgccgggcgt
    16921 ttatgccacg gtggaaggcg aagtgctgaa ctgcaatatc tccttcggcg gtcggcggat
    16981 gatgacctgc cagatcagcg acggttccgg catcctcacc atgcgctttt tcaatttcag
    17041 cgcggcaatg aaaaatagcc tggcgacggg ccgccgtgta ctggcttatg gcgaagcaaa
    17101 gcgcggtaaa tatggtgcgg agatgatcca cccggaatac cgcgtgcagg gcgatctcag
    17161 cacgccagaa ttacaggaaa cgctcacgcc ggtttatcca acaacggaag gcgtaaagca
    17221 ggccacgctg cgtaaattaa ccgaccaggc gctggatctg ctcgacacct gcgccattga
    17281 agaactcctg ccgccggaac tgtcacaagg aatgatgacg ctaccggaag cgttgcgcac
    17341 tttgcaccgc ccgccaccga cgctacagct tagcgatctg gaaaccgggc agcatccggc
    17401 gcaacgtcgt ctgattctgg aagaactgct ggcgcacaac ctcagcatgt tagccttacg
    17461 tgccggagca cagcgttttc atgcccagcc gctgagcgcc aatgacacgc tgaaaaataa
    17521 actcctcgcc gccttaccgt tcaagccaac gggcgcacag gcacgcgtag tggcggagat
    17581 cgagcatgat atggcgctgg atgtgccgat gatgcgcctg gtacagggcg atgtaggttc
    17641 cggtaaaacg ctggtcgccg ccctcgccgc gctgcgtgcg attgcccacg gcaaacaggt
    17701 ggcactgatg gcaccaaccg aattactcgc cgagcagcac gccaataact tccgcaactg
    17761 gtttgcaccg ctcggtatcg aagtgggctg gctcgccggt aagcagaaag gcaaagcgcg
    17821 actgtctcag caggaagcca tcgccagcgg tcaggtgcag atgattgtcg gtacacacgc
    17881 catcttccag gaacaggtgc agtttaacgg cctggcgctg gtgattatcg acgaacagca
    17941 tcgttttggc gtgcatcagc gtctggcatt gtgggagaaa ggccagcagc agggcttcca
    18001 tccgcatcag ttgatcatga ccgccacgcc gatcccccgc acgctggcaa tgacggcgta
    18061 tgccgatctc gatacctcgg tgatagatga gctgccgcca ggccgcacgc cagtgactac
    18121 ggtcgctatt cctgataccc gccgtaccga catcattgac cgcgtgcgcc acgcctgcat
    18181 aactgaaggt cgtcaggcgt actgggtttg tacgttgatt gaagagtcag agttactgga
    18241 agcgcaggcg gcggaagcta cctgggaaga gttgaaactg gcgctaccag aattgaatgt
    18301 tggcctggta cacgggcgga tgaaaccagc cgagaaacag gcggtgatgg cgtcgtttaa
    18361 acaaggtgag ctacatctgc tggttgccac taccgttatt gaagtcggtg ttgatgtgcc
    18421 taacgccagc ctgatgatta tcgaaaaccc tgagcgtctg ggtctggcgc agttacacca
    18481 gctgcgtggg cgcgtaggtc gtggcgcggt ggcttctcac tgcgtgctgc tctacaaaac
    18541 gccgctttct aaaacggcgc aaattcgcct gcaagtgctg cgcgacagta acgacggttt
    18601 tgtgattgcg caaaaagatc tggagattcg cggccctggc gaattgttag gcacgcgtca
    18661 gacgggtaat gctgaattta aagtggcgga tttactgcgc gatcaggcga tgatcccgga
    18721 agttcagcgc ctggcgcgac atattcacga acgttaccca caacaggcaa aagccttgat
    18781 agaacgctgg atgccggaga cagaacgtta ctcgaatgcg taaaaggcgg cggtgagaag
    18841 accgccgctt catcggttaa ccggcaaaaa tcggcaacat taaatacagc ttaatcacca
    18901 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    18961 gcgacgggcc aaagcgttca gtgatcgcct gcatgttggc gattgccgtt ggcgttgcac
    19021 cgaggccaaa accacagtga cccgcagcca gcactgccgc atcgtagttt ttgcccatca
    19081 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    19141 ccagcatcgg cagtgccagc gaagccagct cccacagtct cagccccatc aacgccatcg
    19201 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    19261 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    19321 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    19381 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    19441 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    19501 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    19561 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    19621 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    19681 acagccccat cagcggatca agccctaaca ggctagccat accaatacca atggcatttt
    19741 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    19801 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    19861 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    19921 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    19981 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    20041 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    20101 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    20161 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    20221 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    20281 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    20341 aatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    20401 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    20461 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    20521 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    20581 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    20641 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    20701 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    20761 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    20821 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    20881 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    20941 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    21001 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    21061 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    21121 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    21181 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    21241 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    21301 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    21361 gatccagttg actggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    21421 cgtgctgggt ctgttcccgg cagtgagcga ttttgtacaa cacattccag aaccggttct
    21481 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    21541 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    21601 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    21661 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    21721 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctctttc
    21781 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    21841 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    21901 agtggtgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    21961 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg atcaccgttt
    22021 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gtcagcccgc
    22081 gcccctggtg gcaaaaagtg tcgacattgc gctaagcagt cgccaactga ccgaaccacg
    22141 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    22201 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    22261 agaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    22321 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    22381 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    22441 gactaacctg ggtgccgaca tcgcccgcgg gacattaacc ggaaacgcgc agcgtaacgc
    22501 cgacggcagc tggcaagtgg aaaatctgcg catggcggat atccgtctac aaagcgaaaa
    22561 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgctt
    22621 ggaagtcatc gatgctcgtc tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    22681 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caggaaggca aactgtcgat
    22741 gaacgccagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    22801 attttccccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    22861 cagaacgtca gggaactggc tgcgtgacgg taaaacgttg atccttgatg atgcggcaat
    22921 tgccgggctg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    22981 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    23041 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    23101 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    23161 cgcgacattc aatcgtgttg atgttcgtcg cccgtcgctg gcgctgaccg ccaacagcag
    23221 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    23281 aagtgtttca caaacgccac aacgtcagac ccatatcagc ctgaatggac gcggtgtgcc
    23341 ggtgaatatt ttgcaacaat ggggatggcc tgaattacag ttgactggcg acggcaatat
    23401 tcagcttacc gccagtggag atattcaggc caatgccccg ctgaaaccta cggttagcgg
    23461 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    23521 cgtttccagc ggtgaagtta catcgacgga gccggtgcgg taaacatcaa tctcgccggg
    23581 tacgactcag acgtacccgg cattccgtca atagattaca acgtaattgt cagcgcattc
    23641 ccttgaggcg tcaccaccag cccctgctca ctttcagcct gcgaaccgcc ttgcagacca
    23701 tttactttca caacattgcg caagcacagc gtccagttct tcgcctcgcc cgcaccagtc
    23761 acagtaatcg tgttgccagt acgtgctgct tttaaagtaa agatcaccga tccgtcagca
    23821 gcaggcactt cacagacggc ttcatgcccg tcttgcagat taaagaggtg gaatgccgtg
    23881 ccttcgtgcc acacgtaatc gggacgttga tcgttgttgc ccagcgccag tagagtgtta
    23941 tcacgcacat aaacgggcag actcaggaag ccgtgctgct gtttatgcca gcgactaccg
    24001 tcgagttcat cgttgtgcca caggtgtgtc cagcgacctt ccggcaggta gaactgcaca
    24061 tcgcccgctt cagtgaacac cggcgcaacc atcacgttgt cgcctaacat gtattgacgg
    24121 tcaaggtaat cacaagccgg atcgtccggg aactccatca tcatggcccg catcatcggc
    24181 gtaccccgcg cgttcgcacg cgcagcttca cgatacagat acggcatcat gcggcatttc
    24241 agttgcgtga agaagcgcac cacatcacag gactcatcat cgtaggccca cggcacacga
    24301 taagatttgc taccgtgtaa acggctatgg ctggagagca aaccaaacgc gcaccagcgt
    24361 ttgtaaacgt gcgccggagc ggtattttca aagccgccga tatcgtggct ccagaagcca
    24421 aaacctgaaa ggccaataga caaaccaccg cgcaggcttt ccgccattga ttcgtagtta
    24481 gcgtaacaat cgccacccca gtgtaccggg aatttctgcg caccgacgga ggccgagcgg
    24541 gcaaacaaga cagcttcttc ctcaccaacg gtgtccttga gcacgttcca caccagttcg
    24601 ttgtagatgt acgcataatg gttatgcatt ttctgcggat cggaaccgtc aaaccactga
    24661 acatcagttg ggatacgttc gccaaagtcg gtcttaaagc aatcaacgcc catcgcgacc
    24721 agacctttca gtttgtcggc gtaccatttg caggcatccg gattggtaaa gtcataaatc
    24781 gccagacctg gctgccattt atcccactgc catagcgaac cgtccgggcg tttgagtaaa
    24841 tagcctttct cttgtaactc tttaaagacg ggggattttt gaccgatata ggggttaatc
    24901 cagacgcaga ttttcagccc tttcgctttc aggcggcgga tcatcccttc cgggtcaggg
    24961 aaagtcagcg ggtcccactc aaaatcgcac cactggaagg ctttcatcca gaaacagtca
    25021 aagtggaaaa catgcagcgg cagattgcgt tccgccatac catcgataaa gctgtttacc
    25081 gtcgcttcgt cgtagttggt ggtaaatgaa gtggttagcc acaggccgaa ggaccacggg
    25141 ggcggcagcg ccggacgacc agtaaagcgg gtataacgat caagtaccgc tttcggcgtc
    25201 gggccgtcga taacaaagta ttcgagatat tcactctcaa cgctgaactg cactttggag
    25261 actttctccg atcccacttc aaaagagaca cactggggat gattgaccag taccccataa
    25321 ccacggttag tcatgtagaa cgggatattt ttatacgcct gttcagtact tgtgccgccg
    25381 tcccggttcc aggtctctac cgtctggcca ttgcgcacca gggcagtaaa gcgctctccc
    25441 agaccgtaaa ctgtttcgcc aacgccaaga tcaagccgct caaacatata attgcgttga
    25501 ttattcgtgt cctgcacgta gccattattt ttcacctgac taccggtaat acgttcgccg
    25561 ttgcgcagaa aatccagtga ccagaactca cctttgctga cacgcgcgct taagttgcca
    25621 cttttaaact cagcataacg ttctgtgttt tcgattgtga ccttcacgtc ctgcaaaata
    25681 ttgagcggat aatgaggacc gttattcagc gccccctgaa aatactcaat ccgcacaccg
    25741 acaatacctt cctgtgggga gaaaaagcgc agcgtaaata aaggcgtatc aagctgccag
    25801 gtacgttcac gcacatcacg gggggcagca tagaccacca tttcattatc ctgctgttca
    25861 acctcgaata cctgaagcgg gtgaatcaaa ttgaggccag gttgaatcaa ccagtttcca
    25921 tcgctaattt tcattgccgt tccttagttc tgcacttctt gagaggtgaa ttgttgctgg
    25981 caacggcgtt taccctgagc cagctgttcc ataacggttt tcagattgtg cgtcgtgagt
    26041 gagtagtagc gtttagcgat aatcgcgctc agcaaataac agatcgccgg aacaatcgtg
    26101 aatagcgcaa taatgatgct aatcgtggcg ctgttctgcg ctttttctgc cgcatcatat
    26161 ccgccataag ccagcatcca gccgataaga gcgccgccga aggccaaccc cagtttgagc
    26221 acaaacagcg tgccagcaaa actgatcccg gtcaggcgtt taccattgca ccactcgccg
    26281 tagtcgacgg tatcgaacat cattacccac tggataggtg tcaccagttg atgcaacaca
    26341 ccaatcacga agatgaagac aaacatagtg atgctggcct gcatgggaac aaagaacatc
    26401 gcgaggctaa tcactgccag cagggcgttc gtccaccaga agatagtgac tttacatttc
    26461 cagtcggtca gaggttttgc cagtgcggaa ccaatcaggt tacccacgca ataagtggtg
    26521 agaaaagcga caaacacttc cggcgtgccc aaaatccatg tgacgtaata catcatcgcc
    26581 ccaccgcgta cgcacaccgc caggatattg aaaatggtta gtaaaccgac aatccgccac
    26641 tggtcgtttt gccagatatc acgtaaatct tcccgcatag acgttgttgt aggtggtgct
    26701 tcaacgcgtt ctttagtggt gaagaaacaa aatgccagca tcatgaatgc caccacggaa
    26761 aggaccacga taccgccctg gaaaccgagt ggtttattat caccgccaat taaattaacc
    26821 agtggcatca tcagaacagt agaaagcatg cctcccgcgg tcgccagcac aaaacgccag
    26881 gattgcagcg agatacgctg agtcgggtca ttggtgatta caccacccaa tgcgcagtaa
    26941 gggatattga cgacggtata aagtaaggta agttagggtg taagtaattg ctgcatagat
    27001 cattttgccg ttcatactga gatctggcgt gctataggcc agtacacaga cgatcccgaa
    27061 tggcagtgcg ccaaacagta cccacggacg aaatttaccc cagcgagagc gcgttcgatc
    27121 ggccaacaac cccatgcaag gatcggaaat cgcatccagt gcacgagcga ccaaaaacat
    27181 ggttccgaca aatccggcag gaatgccaaa aatatcggta taaaagaaca tcatatataa
    27241 cattacgtta tcgaaaataa tgtggctggc ggcgtctccc atgccataac caattttctc
    27301 tttaacggac aacacttcac tcttcatctg cttttccttg atgctatagg ggtgctgaga
    27361 ccgataacgc attttgtaaa ccatccctgt gagagcgaat attccgatat ctggttatta
    27421 atttatgatt cttgttttat gtgatcgtgg tagcgttaat tccgctcata tatcattgta
    27481 aaatatgggt tttatataaa ttataatgct ttcgtgataa tacgctgcgt ttattaggcg
    27541 gaaaaaactg atctggggga tgtagaaact caaggaagta gctataatgc gccccgcctc
    27601 catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg aggcggctgg acttcaaatc
    27661 cagttggggc cgccagcggt cccgggcagg ttcgactcct gtgatcttcc gccagtttac
    27721 ctcttctgaa ctctcccaat gtcaataaat ctcttctaat cccttgtata tcagtattta
    27781 tttgtatcct gaggtcaacc tacttcaccc cgtatcaagt ttcttttggg ggtactttag
    27841 ggggtgttat ttcctattga aatacaagca cccccataat aggaaaaatg tgatacataa
    27901 caacggcttt taacaatatc tttgttatac aaggggtgta ttgggggtat caggttagtt
    27961 actggcagaa atccccctca ggaacatgaa ttcatcaaaa gccggaggat gtcatggcat
    28021 taaccgatat caaagtgaaa accgcaaaac ccaaagacaa accctataag cttgctgatg
    28081 gcggcggtat gtatctactg attaacacta atggttctaa atactggcgc atgaagtacc
    28141 gctttgctgg taaagaaaag atgctttcta ttggcgtata ccccgatgta acgctagccg
    28201 atgctcgcga gaaacgcagt gaagccagga aactcctcgc tgctggaggc gatccagggg
    28261 aggcaaaaaa ggaagaaaaa atcgcgcagc aaatgagtct gaaaaatacg ttcgaggcta
    28321 ttgctcgcga atggcaccag ttaaaagctg atcggtggtc attacgctat cgtgatgaaa
    28381 tcattgatac ctttgaaaaa gacatttttc cctacattgg caagcgccct attgccgaaa
    28441 tcaaaccaat ggagttactg gaagcactgc gtaaaatgga aaaacgtgga gctctggaga
    28501 aaatgcgcaa ggtccgccag cgctgtggtg aggtgttccg ctatgcaatt gttactggcc
    28561 gggcagacta taacccagcc cccgatcttg ccagtgcttt agctacaccg aaaaaagtac
    28621 attttccctt tcttactgct aatgaacttc cccacttcct caacgatctg gcgggttata
    28681 ccggaagtat aatcactaag acagctactc agatcattat gttaaccggc gtacgaacac
    28741 aagaactgcg ttttgcacgc tgggaagata tcgattttga gacaaaattg tgggagatcc
    28801 cggcagaagt aatgaagatg aagcgacctc atattgtgcc gctctctgag caggtcatta
    28861 tgctatttaa gcagcttgaa ccaatctcaa aacatcatcc cctagtcttt atcggtagaa
    28921 atgatccccg caaaccaatt agtaaggaaa gcattaacca agtaattgaa ttacttggat
    28981 ataagggaag actcacaggg cacggtttca gacacaccat gagtacaatt ctgcatgaac
    29041 aaggctttaa ttccgcctgg attgaaatgc agttagctca tg
//
LOCUS       DADLZM010000039        28463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLZM010000039 DADLZM010000000
VERSION     DADLZM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.039"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="JNY73_001680"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="JNY73_001680"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY8047717.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNY73_001681"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNY73_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY8047718.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="JNY73_001682"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="JNY73_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY8047719.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /locus_tag="JNY73_001683"
     CDS             2778..4127
                     /locus_tag="JNY73_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8047720.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="JNY73_001684"
     CDS             4278..5927
                     /locus_tag="JNY73_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY8047721.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="JNY73_001685"
     CDS             complement(6081..7373)
                     /locus_tag="JNY73_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY8047722.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNY73_001686"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNY73_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY8047723.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVMLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIISFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="JNY73_001687"
     CDS             complement(9197..9511)
                     /locus_tag="JNY73_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY8047724.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNY73_001688"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNY73_001688"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY8047725.1"
                     /translation="MSQIHKHTIPANITDRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="JNY73_001689"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="JNY73_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY8047726.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAVLDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="JNY73_001690"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="JNY73_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY8047727.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="JNY73_001691"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="JNY73_001691"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY8047728.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="JNY73_001692"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="JNY73_001692"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY8047729.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAVCGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="JNY73_001693"
     CDS             15750..17468
                     /locus_tag="JNY73_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY8047730.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLAAFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="JNY73_001694"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="JNY73_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY8047731.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="JNY73_001695"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="JNY73_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY8047732.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="JNY73_001696"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="JNY73_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY8047733.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="JNY73_001697"
     CDS             complement(20170..20859)
                     /locus_tag="JNY73_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8047734.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNY73_001698"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNY73_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNY73_001699"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNY73_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY8047735.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNY73_001700"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNY73_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNY73_001701"
     CDS             complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNY73_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY8047736.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGDDSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAALEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27862..28137)
                     /locus_tag="JNY73_001702"
     CDS             complement(27862..28137)
                     /locus_tag="JNY73_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047737.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28346..>28463)
                     /locus_tag="JNY73_001703"
     CDS             complement(28346..>28463)
                     /locus_tag="JNY73_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047738.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6485 a         7207 c         7641 g         7130 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcagtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttggtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctagagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag attatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcgg ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tccttttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagctgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcatcaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgt gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg tgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggtgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaacttaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cggcgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgtgct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttcga gcgccgcacg ttgcgccacc agcgcatcga cgccgctgac
    27301 ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt ctgcggcgcg
    27361 ctgcgccgtt cttgcacggt caacgtcttc tgctgagaca aaaccctctt tcagtaatgg
    27421 ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt
    27481 ggcattaacc gagtcgtcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat
    27541 ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg
    27601 cgggtcgatg cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag
    27661 ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc
    27721 gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc
    27781 caacgcaacc actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc
    27841 catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc
    27901 aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac
    27961 tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata
    28021 ataagcgccg atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg
    28081 aattatccag gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt
    28141 aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg
    28201 tcagtatggc tattttctcg atgaataaaa atagctttat tcagccaccg cattcataac
    28261 ttatatattt tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct
    28321 tcagcgcccc caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga
    28381 aattatccag acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc
    28441 tcttcgcttt caccagatcc gcc
//
LOCUS       DADLZM010000040        27687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLZM010000040 DADLZM010000000
VERSION     DADLZM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.040"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..704)
                     /locus_tag="JNY73_001704"
     CDS             complement(<1..704)
                     /locus_tag="JNY73_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047739.1"
                     /translation="MGPLPRKGNMKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRL
                     ITQRRKKHWDESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWS
                     SDDGFSVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGF
                     EDFFIAVDPKTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLL
                     AMVDVLLCYLEKGAEYV"
     gene            complement(889..1203)
                     /gene="pspE"
                     /locus_tag="JNY73_001705"
     CDS             complement(889..1203)
                     /gene="pspE"
                     /locus_tag="JNY73_001705"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY8047740.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="JNY73_001706"
     CDS             complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="JNY73_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY8047741.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="JNY73_001707"
     CDS             complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="JNY73_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY8047742.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="JNY73_001708"
     CDS             complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="JNY73_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY8047743.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="JNY73_001709"
     CDS             complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="JNY73_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY8047744.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2980..3957
                     /gene="pspF"
                     /locus_tag="JNY73_001710"
     CDS             2980..3957
                     /gene="pspF"
                     /locus_tag="JNY73_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY8047745.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLQDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="JNY73_001711"
     CDS             complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="JNY73_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY8047746.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5380..6660)
                     /locus_tag="JNY73_001712"
     CDS             complement(5380..6660)
                     /locus_tag="JNY73_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY8047747.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="JNY73_001713"
     CDS             complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="JNY73_001713"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY8047748.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="JNY73_001714"
     CDS             complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="JNY73_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY8047749.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="JNY73_001715"
     CDS             complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="JNY73_001715"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY8047750.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9859..11276
                     /locus_tag="JNY73_001716"
                     /pseudo
     CDS             9859..11276
                     /locus_tag="JNY73_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
     gene            11425..11589
                     /locus_tag="JNY73_001717"
     CDS             11425..11589
                     /locus_tag="JNY73_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047751.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11579..12964
                     /gene="puuP"
                     /locus_tag="JNY73_001718"
     CDS             11579..12964
                     /gene="puuP"
                     /locus_tag="JNY73_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY8047752.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVHQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13098..13343
                     /locus_tag="JNY73_001719"
     CDS             13098..13343
                     /locus_tag="JNY73_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY8047753.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTHLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13656..15299
                     /gene="sapA"
                     /locus_tag="JNY73_001720"
     CDS             13656..15299
                     /gene="sapA"
                     /locus_tag="JNY73_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY8047754.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15296..16261
                     /gene="sapB"
                     /locus_tag="JNY73_001721"
     CDS             15296..16261
                     /gene="sapB"
                     /locus_tag="JNY73_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY8047755.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16248..17138
                     /gene="sapC"
                     /locus_tag="JNY73_001722"
     CDS             16248..17138
                     /gene="sapC"
                     /locus_tag="JNY73_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY8047756.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17138..18130
                     /gene="sapD"
                     /locus_tag="JNY73_001723"
     CDS             17138..18130
                     /gene="sapD"
                     /locus_tag="JNY73_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY8047757.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18132..18938
                     /gene="sapF"
                     /locus_tag="JNY73_001724"
     CDS             18132..18938
                     /gene="sapF"
                     /locus_tag="JNY73_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY8047758.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19006..19359
                     /locus_tag="JNY73_001725"
     CDS             19006..19359
                     /locus_tag="JNY73_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY8047759.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19727..20515
                     /gene="fabI"
                     /locus_tag="JNY73_001726"
     CDS             19727..20515
                     /gene="fabI"
                     /locus_tag="JNY73_001726"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY8047760.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20659..21786
                     /locus_tag="JNY73_001727"
     CDS             20659..21786
                     /locus_tag="JNY73_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY8047761.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21854..23788
                     /locus_tag="JNY73_001728"
     CDS             21854..23788
                     /locus_tag="JNY73_001728"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY8047762.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23866..23968)
                     /locus_tag="JNY73_001729"
                     /pseudo
     CDS             complement(23866..23968)
                     /locus_tag="JNY73_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24022..25996
                     /gene="pdeR"
                     /locus_tag="JNY73_001730"
                     /pseudo
     CDS             24022..25996
                     /gene="pdeR"
                     /locus_tag="JNY73_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26144..26317
                     /locus_tag="JNY73_001731"
     CDS             26144..26317
                     /locus_tag="JNY73_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047763.1"
                     /translation="MSEFDTQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26407..27156
                     /locus_tag="JNY73_001732"
     CDS             26407..27156
                     /locus_tag="JNY73_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8047764.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27425..27643
                     /gene="osmB"
                     /locus_tag="JNY73_001733"
     CDS             27425..27643
                     /gene="osmB"
                     /locus_tag="JNY73_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY8047765.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         6438 a         7149 c         7117 g         6983 t
ORIGIN      
        1 acatactcag cacctttctc aaggtaacag agtaaaacat ccaccatcgc cagcaacact
       61 tcagggctac ggtaattcag gtcaatttga tcgtcactaa aggtggtcca taaatggcgc
      121 gtggaattat catccatttg aaacggcgtt aatagcggta acgcacgcgg acgggtgacg
      181 gcactgagat cggttttcgg atcaaccgca ataaagaaat cttcaaaacc cggaagctgt
      241 tgtaaatagt ttttaaacca ttcacttttt gcagacatat ggttgcagac aaaatcaaac
      301 attaaatgac tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg
      361 acctgatgat aatcaattac cgaaaatcca tcatcagatg accatgggta aaaaggtaat
      421 aaatgaacat gtgaaaaaat gctttgcagc cattgatggt agaactgatt aaatatgggt
      481 agtggtttta aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt
      541 tcatcccagt gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt
      601 acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatcc
      661 gtaattttct gtttcatgtt gcctttccgt ggtagcggtc ccatgaggcg agagactact
      721 ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt
      781 taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatagggat gtaaataagg
      841 tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac
      901 cttcggcatt gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc
      961 gctaaggatc tcttttgcct gcccggactg gcgtccggca ttgcaataca ctttcacggt
     1021 gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt
     1081 gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg
     1141 ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa
     1201 catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa
     1261 tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc agcccgactt
     1321 aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc aactgatttt
     1381 atcgcccagc ccgccacacc cgccgggcca tagcgcagtg cggtaagaag taccagcttg
     1441 cctgctaatt tgaaaccaag cttcaccttt tgcccggcct gttgccagcg agtattcata
     1501 actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac
     1561 gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca
     1621 attcgctgct ggaaggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg
     1681 caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca
     1741 gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca atcccggcgc
     1801 agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc
     1861 ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt ccagcgcctg
     1921 aatacgttcg cgcatccgtt ttgcttcatc aaccagttgc gctaatcgct gctgctcact
     1981 ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa
     2041 aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca taagtactcc
     2101 ttagacgatg agcggcggcg cgttcggacg ccgcctggat gttattattg attgtcttgc
     2161 ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc tttcagttcg
     2221 gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc
     2281 atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca
     2341 ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatgacg taacatcaat
     2401 gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcgccaat ctctttcttc
     2461 atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagggactt aatcagatcg
     2521 gtcagtttct gtttttcaat taacgctgca cgtgccagat cctctctctc tttcagcagc
     2581 gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga
     2641 gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc aaccagtgta
     2701 tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc tttctctaac
     2761 agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg
     2821 tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca
     2881 attttttatc tttttgattt ataaagatct gattgaagaa tcaacagcaa catgccagga
     2941 tgaattagcg aattacacta acaagtggcg aatttcatca tggccgaata caaagataat
     3001 ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg
     3061 ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagtt gattgccagc
     3121 cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg
     3181 ttaaatgaaa atctgcagga ttccgaactg tttggtcacg aagcgggggc gtttaccggt
     3241 gcgcaaaaac gtcatcctgg gagatttgaa cgtgccgacg gcggtacgct atttcttgat
     3301 gaactcgcta cggcaccgat gatggtgcag gaaaaattat tgcgtgtgat tgagtacggt
     3361 gaactggagc gcgttggcgg tagccagccg ttgcaggtga atgtgcggtt ggtatgcgcg
     3421 acgaatgccg atctcccggc gatggtcaat gaaggtactt ttcgcgctga cctgctcgac
     3481 cggctggctt tcgatgtagt gcaactgccg cccctgcgcg agcgcgaaag cgacattatg
     3541 ctgatggcag aacactttgc cattcagatg tgtcgggaaa tcaagctgcc tctgttcccg
     3601 gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg aaatattcgt
     3661 gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga ttatccgctt
     3721 gatgacatca ttattgatcc ctttaaacgg cgtccacctg aagaagccat cgccgtttca
     3781 gaaaatacct cgcttccgac gctgccgctg gatttacgcg agtttcagat gcagcaggaa
     3841 aaagagttgc tgcaactcag tttgcaacag gggaaatata accagaagcg cgcggctgaa
     3901 ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca gatttagcga
     3961 agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat acataatcaa
     4021 tacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt gggctattaa
     4081 tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc cgggatggtc
     4141 agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag gagcagcccc
     4201 tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc
     4261 ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc aatggccgga
     4321 acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg attcgcgcgt
     4381 tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc caccgccagc
     4441 gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat aatattcgcg
     4501 ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat cgtcattaag
     4561 tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc aaagccgctt
     4621 tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg aatggcggca
     4681 accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc gaaaataatg
     4741 gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc gtcgagggag
     4801 tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac cgaaccaggg
     4861 aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat ggtcatatat
     4921 gtacgaccgt ggaagccacc gctaaacgca atcacgccag gtcgtccggt atgggcgcga
     4981 gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc ggttttagcc
     5041 tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac
     5101 ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc
     5161 agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat
     5221 tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa gaagttacac
     5281 atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc attgttgctc
     5341 atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca gacgatcgcg
     5401 caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg
     5461 gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac cgcgcagcag
     5521 ttcagcaatc aaacgcccgg caagatgggt acaggtgacg ccgtggccgc tgtaaccctg
     5581 catgtaatag atgttggtat caaggcggcc gaactgcggc atgcgcgaca aggtcagcag
     5641 gaagttgccc gtccagcggt aatcaatttt cacgcccttc aactgaggga aggtttttag
     5701 tagtttcggt accacaagac gctcaacgtc atccgggttg cgtgcaccat acactacacc
     5761 cccgccgtac agcaggcggt tgtcggcggt aagacggtag taatcaagca gatagttaca
     5821 gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg acagccgttc
     5881 ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gctagttccg gctctacttt
     5941 atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct gaccattggc
     6001 agtccgaacc acggctggcg tggtgtgctg gatctgcgtc acggcggaaa gctcatacac
     6061 gcgcccgccg ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta gcgggtgaat
     6121 atgcccaccg ctgtgatcca gcagcgcacc agtgtagcga tcgctggcga cctcgcggcg
     6181 aatagcgttg gcgtccagca gctccagctg tttattgccg tagcgttccc agttctcttt
     6241 ctgctcttcg agtgtggcga gttgtttatc attcatcgcc acaaacagac cgccggggcg
     6301 gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcgc caccctcgaa
     6361 catcatgctg ccgagcatac gggcggtgtc catgccgtag cttttttcga tcacgtcgat
     6421 gtcgcggctg taggagttca caagctgccc gccattgcgc ccgcttgcgc caaagccgat
     6481 gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca gcgcggagga
     6541 gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact cattcagtgt
     6601 gtcgaatggt gcatatttat tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat
     6661 ttcaggcctc caggcttatc cagatggttt tcagttcagt gaatttttca agggcatgca
     6721 gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg gtcatatcgc
     6781 cgtcgttgta gttattgacg aagacggaac cggctttcag gcgtcggctc atgcggtgcg
     6841 cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg tcgttggcaa
     6901 gctgcagcgc ctgatcttct gatgtgaaac gggtgaccac cagcaccgga ccgaaaatct
     6961 cttcgcggct taaggacgca ttcgggtcca catccacgaa gatggtcggg ccgatggccg
     7021 ctgccagccc ggcgttgcga ccatccagca gcagttgccc cttgccttcg ccttcacgaa
     7081 taaaggtgtg taccgagtca gcgtgagcgc tgtcgattaa ggtgcccatg gtggttgcgg
     7141 gatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg gctaagaatt
     7201 catcggcgat gctctcttcc agtaacaaac gcgttccggc gatgcacacc tggccctggt
     7261 tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga caatcggcga
     7321 aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac acgtttcatg ttgctatcac
     7381 ccgcgtcttt cagcagttgt ttacccgtac gcgtggagcc ggtaaaggcg atggcatcga
     7441 tatcgttatg acgcgaaagc gcctgccccg cttcatgacc aaaacccgtc accacgttca
     7501 acacaccatc cggcaagcct gcttctttcg ccagccccgc gagacgaatc gcactgagcg
     7561 gtgatttttc agacggtttt agaatcacgc tgtttcccgc cgccagcgcc gggccgagtt
     7621 tccagcaagt cagcaacagc gggaagttcc acggcacgat ggcggcaatc acgccgaccg
     7681 gttcacgcac gatcatcgcc agctcatggc tactggtggt cgccacttcg ccatacactt
     7741 tgtcgatcgc ttcggcgtac cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca
     7801 gactgtgacg aatcggtttg ccggtgtcga gagtttccag cagtgccagc tcttcggcgt
     7861 gggcttccat taaatcggcg agtttattca gtaccgcttt acgtttagcc ggagaagaga
     7921 gtgaccagtc gccgcgttca aatacgccgc gagctgcgct caccgcacgg tcgatatcga
     7981 cgctcttgcc gcgggcaatt ttcgccagcg gtgcctgggt gaccggatca acggtttcaa
     8041 aggtttcatt ttccgccgca gcagtatatt caccgttaat aaataagcgg ttttcaatgg
     8101 cgagacttaa cgctttatcc tgccagtaag ccagatgatg aaaattcatt atgactcctg
     8161 tttcacgtct atcagatata tgcagtttgt gaacgtcagg gcgcagagat ccccgggctg
     8221 catgcccgtt tatttctggc gacattcttt atggattgcc gggcgcattg cgcttacccg
     8281 gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa ttcggcaaat
     8341 acctgccgac gtattactga aactgtgcgg gatgccggta ttaatggcat aactttgccc
     8401 cgcgacgagg tggtaatcct gaccattaat cgtcagaaca atttcacctt ccagtactgt
     8461 gcctatttcc tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtacgt
     8521 ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac
     8581 accctgactg cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg gctcatcagg
     8641 tttttccggc tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg
     8701 cagcgtactg atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt
     8761 cagcccggag agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga
     8821 caagcgtttt cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt
     8881 ggtcagagtc gctgtttttc agcgatatgg tgctgccaag cggtgataaa gccctcgaac
     8941 aatatacgcg aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgcccagc
     9001 gcaaaaggat gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca
     9061 acacgcaacc gtgggctaac gaccttcgcc ccttgtccat gtagagagtt tacccaaaag
     9121 ttgctacatt caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc
     9181 gacggtgcat attgctgttc caccggcagt tcgggatctt cccgatgctc cagcaattca
     9241 ggctgttcgc acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcttgtaaa
     9301 ccccggcaga tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat tatcgccatg
     9361 ctcagaagat cacgcccggg atcggcgtca ggctcatcgc cgttttcacc atataggtgc
     9421 ggctgcacat tgctgggact actaggaaga taaatgccat cgagtttcgg caaaagttgt
     9481 tcaagtaacg acggttccgc cagcgcatgt ggtagcgcaa ttggcaagcc gcctgcgtgg
     9541 atgatggcat tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc
     9601 ctgcacatta cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca
     9661 aagtggacta aattatcgcc attactgcgc tttaaatgac ctttatgttc aatatttttt
     9721 caatctagca gtggattttc atttttgcaa actaaattta acatttgaca aacatttggt
     9781 ttgcatacag attcgaatga tggtcattat attttacgct ttgataacga gcggaaaaca
     9841 aaccaaaggc gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag
     9901 tcagaagaga ggcggggtag cgcctttacg caggaagtga aacgctacct ggagcgctac
     9961 ccgaatacgc aatatgttga tgttttactt actgatttga atggttgctt ccgtggaaaa
    10021 cgtatccctg tttcaagcct gaagaagctc gagaaagggt gttatttccc ggcctcggta
    10081 tttgcaatgg atattctggg caacgtagta gaagaagcag gtctgggtca ggaaatgggc
    10141 gagccggatc gtacctgtgt tcctgttctc ggttccttaa ctccttctgc cgccgatcca
    10201 gagtttatcg atcagatgct cctgaccatg gtcgatgaag atggcgctcc ctttgacgtt
    10261 gagccgcgga acgttcttaa ccgcctgtgg cagcagctgc gccagcgcgg attgttcccg
    10321 gttgtagcgg tagagctgga gttctattta ctggatcgcc agcgcgacgc tgaaggggat
    10381 ctgcaaccgc cctgcgcgcc tggcaccgat gaccgcaata cacaaagcca ggtttactcc
    10441 gttgataacc tcaaccactt cgccgacgtg ctcaatgata ttgatgaact ggcgcagttg
    10501 cagctgattc cggcagatgg cgcggtcgct gaggcctcgc cgggtcagtt tgaaatcaac
    10561 ctttaccata ctgataacgt gctggaagcc tgcgatgatg cgctggcact aaaacgcctg
    10621 gtgcgtctga tggcagaaaa gcataagatg cacgccactt ttatggcgaa gccgtatgaa
    10681 gagcacgcgg gcagcgggat gcatatccat atcagtatgc aaaataatcg tggcgagaat
    10741 gtgctttctg acacagaagg cgaagattcg ccgctgctga aaaagatgct cgccgggatg
    10801 attgacctga tgccgtcgtc gatggcgttg ctggcaccaa acgtgaactc gtatcgccgc
    10861 ttccagccgg gaatgtatgt gccgacgcag gcgtcgtggg gccataacaa ccgcaccgtc
    10921 gccctgcgta ttccgtgcgg tgaccgtcat aatcaccgcg tggaatatca cgtggcgggt
    10981 gccgatgcca acccatatct ggtgatggca gcgatttttg ccggtatttt gcatggcctt
    11041 gataacgagc tgccgttgca ggaagaagtc gaaggcaacg ggctggaaca ggaaggctta
    11101 cccttcccga ttcgccagag cgatgcctgg gtgagtttat cgagaatgat cacctgcgcc
    11161 gctatttagg cgaacggttt tgccatgtgt atcacgcctg taaaaatgat gagctgttgc
    11221 agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac gcgtaattcc
    11281 ccttgcgggg ttgtttcatc ggcctgagcg gagccaggcc tgacatttgc tggtaatcca
    11341 gacatttccc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg ttaacgatta
    11401 agactgtgac aaggaaatga gatgatgccg atgattaaat ccccgcatgg cgagggaggc
    11461 tgcgtatgcg cccctcctgc tactgactgg actccgcccc ctttgcttcc cttgctaaac
    11521 aggtttgatt tcaggtcgac acgaccgcaa accttattac gcaggggagg cagcaattat
    11581 ggctattaat tcaccactga atattgctgc gcaacccggc aaaacccgtc tgcgaaaatc
    11641 actgaaattg tggcaggtgg taatgatggg tctggcctat ctcacgccga tgaccgtatt
    11701 tgatactttt ggtattgtct ccggcattag cgacggtcac gttccggcgt cctatttgct
    11761 ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc aaactggttc accagtttcc
    11821 ggaggccggt tcggcctata cctacgcgca aaagtcgatt aacccgcacg tcggatttat
    11881 ggtcggctgg tcatcactgc tggattatct ctttttgcca atgatcaacg ttttgttggc
    11941 gaaaatctat ctctccgccc tcttcccgga agtgccgccg tgggtgtggg tggtaacctt
    12001 cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac ctggtcgcta acttcaatac
    12061 cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt atcttcctgg tggttcaggg
    12121 actgcataaa ggagaaggcg ttggcaccgt ctggtcactt cagccgttta tcagcgagaa
    12181 cgcgcacctg atcccgatta ttaccggtgc gacgattgtc tgtttctcgt tcctcggttt
    12241 cgatgcggtg accacgcttt cggaagagac gccggacgcc gcacgcgtga tcccgaaagc
    12301 catcttcctg acggcggtat acggcggcgt tatctttatc gcggcgtcgt tctttatgca
    12361 gctgttcttt cccgatatca gccgctttaa agacccggac gccgcactgc ctgaaattgc
    12421 gctgtacgtc ggcggcaagc tctttcagtc gattttcctc tgcaccacgt ttgtgaacac
    12481 attagcgtct ggcctggctt cacatgccag cgtgtcgcgt ctgctgtatg tgatggggcg
    12541 tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac ccaaaatggc ggactccggc
    12601 actcaacgtc attatggtcg ggattgtcgc gctgtcggcg ctgttctttg atttagtcac
    12661 cgcgacagca ctgattaact tcggtgctct ggtggcgttt accttcgtga atctgtcggt
    12721 gtttaatcat ttctggcggc gtaaaggaat gaataaaagc tggaaggatc acttccacta
    12781 tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg ctgtgggtta acctagagtc
    12841 aacgtcactg acactcggtc tggtatgggc ttcgttaggc ggcgcatatt tgtggtatct
    12901 gatccgccgc tatcgcaaag tgccgctgta cgaaggtgac agaacgccgg tgagcgaaac
    12961 gtaatagccg gatgcgcggc gcattatcct gcctgagggt aacgcgccgc gccattaact
    13021 ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc ttagcgcatt
    13081 aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat attgccgatg
    13141 agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg ctggcccact
    13201 acttccagtt actgctcacc catttgatga acaatgaaga gatcagcgaa gaagcccaac
    13261 atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc gcagagtttt
    13321 tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt atcgttgtcc
    13381 ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg tgctttaatg
    13441 ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt tatagttata
    13501 gagaaatcac gggtacgaat gttgaataga tggcgacgcc acctgttcaa cgaccaacca
    13561 cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat taaatattag
    13621 taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta tcgtctcttt
    13681 tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc cccccgcatg
    13741 ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac acctttaacc
    13801 catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt tatgatcgac
    13861 tgctggatgt cgatccctat acctatcgcc tgatgccgga acttgccgaa agctgggaag
    13921 tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg tttcaaaaaa
    13981 ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt accttccagc
    14041 gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc ccctacttcg
    14101 acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat cataccgttg
    14161 agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc cattatgctt
    14221 cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag gagcaactcg
    14281 accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc gggcaattta
    14341 ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg caggtagtgg
    14401 tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg gaatgcgacg
    14461 ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg cgcttgcgtt
    14521 taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc aaaccgccgc
    14581 taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag cgcctgatgc
    14641 aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc gcctcgtggg
    14701 cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc gaacagttga
    14761 aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt tcgcaggcgt
    14821 ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg caggttggcg
    14881 taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg atggatatga
    14941 gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac agtttcttcc
    15001 gtccgttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac tggtgcgatc
    15061 cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggca gcgcgtattg
    15121 aagcctatga cgaagcgcag agtattctgg cgcaggaatt acctattctg ccgctggcgt
    15181 cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt agcccgtttg
    15241 gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa aaaccatgat
    15301 tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc tgaccttcgt
    15361 tggcttcagc ttgagctatt tcactccaca cgcgccgtta caaggcgcgt cactgtggaa
    15421 tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt ccagcattaa
    15481 cggtcaacca attgccgaac agttaaaaga ggtcttcccg gcgacgatag aactgtgcat
    15541 cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg ccgggattac
    15601 gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct tttcaatccc
    15661 ggtgttctgg ctggcgcttc tgttgacact gttttgttca ctcacgctag gttggttgcc
    15721 cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt ttgcgttgat
    15781 tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg caatccgcca
    15841 tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga tccgcctgat
    15901 gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg cgacccgcgg
    15961 tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac cgccggttat
    16021 tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca ccgaaatggt
    16081 ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc aggattacgc
    16141 agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta acgtgatttc
    16201 tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg ccttacgata
    16261 gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc aaattttata
    16321 gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg actggctgta ctgtgtattt
    16381 ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat caattattgc
    16441 cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac gacctcggac
    16501 gtgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc gcatttgtgg
    16561 tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg gcgacacacg
    16621 gcctccgctc agcggtgctt aaccatattc tggatacatt actggcgatc ccttcgctgc
    16681 tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct atgtttgctg
    16741 tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg catgacgaac
    16801 tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg aatattctct
    16861 ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc cgcgcactgt
    16921 cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca caactcccct
    16981 cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc gccccgtgga
    17041 ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac ctccttggcg
    17101 atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg atattcgtaa
    17161 cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc gggtaagtat
    17221 gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg gcaagagttt
    17281 gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg ctgaccgtat
    17341 gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca aactggttgg
    17401 ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt cagaacgtgt
    17461 gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt ggtggcagcg
    17521 ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga ttaaagatca
    17581 caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc agaaagtgat
    17641 gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac cgacaaactc
    17701 aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca accaaaacag
    17761 caataccact attttgctta tcagccatga cttacaaatg cttagccaat gggcggataa
    17821 aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg agttggtgac
    17881 gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact tcggcagcgc
    17941 gatgccgcat aaaagtcgcc tcaatactct acccggcgct atcccattgc tggaacagtt
    18001 accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat gcattgtgac
    18061 gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc tgaacatgga
    18121 gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa acgttccgct
    18181 accggaccgg ttggtttcgt cgtcagaccg tagaagcggt aaaacccttg agctttacgc
    18241 tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa tccacactgg
    18301 caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt gacgatcatc
    18361 cgctgcattt tggcgattat tccttccgta gtcagcgtat tcgcatgatt tttcaggatc
    18421 cttcgacctc gttgaatccc cgtcaacgta tctcacaaat tctggatttt ccactgcgcc
    18481 tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg atgcgtatgg
    18541 ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc ggacaaaaac
    18601 agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt gccgatgaag
    18661 ccctcgcttc actggatatg tcgatgcgtt cgcagttgat taatctgatg ctggagttac
    18721 aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg atgaagcaca
    18781 ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc agcaccgcgg
    18841 atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg cactttggcg
    18901 aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc tccttccaga
    18961 ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat ggataaaata
    19021 aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa gctctggcga
    19081 tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc ggtggggagc
    19141 tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga tggtgggcag
    19201 catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc gcagggctgg
    19261 accgtgctgc gtttctggaa taacgagatt gattgtaatg aggaggcggt gctggagatt
    19321 attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg ggccgaccga
    19381 gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc ggcaaggtct
    19441 gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt ttgttgctgg
    19501 ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt tagggtaagg
    19561 ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta tagctactca
    19621 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
    19681 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
    19741 cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct acggtatcgc
    19801 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
    19861 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt
    19921 tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
    19981 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
    20041 taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
    20101 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
    20161 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
    20221 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
    20281 cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag acttccgcaa
    20341 aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt
    20401 gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtagt
    20461 ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga aataatcgtt
    20521 ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
    20581 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
    20641 tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat
    20701 gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga agccgcaaag
    20761 gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc ccccttcctt
    20821 tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca gcttaccgtg
    20881 acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac ggttgctcag
    20941 gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc gctccccgga
    21001 cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata tgcccgccaa
    21061 ctggctagct cgcctgctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt
    21121 cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg ctttcaggcg
    21181 cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt acccgggctg
    21241 gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc
    21301 agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc agaactttgc
    21361 caactggccg aaacacactc tctccaccct atttcacttt cccttctcga aaagttgtta
    21421 aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt atgcgcgcgt
    21481 ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga atataaaaaa
    21541 atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga ccgtcattct
    21601 gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
    21661 gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat tattaatctg
    21721 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
    21781 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
    21841 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
    21901 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct ttggcttcct
    21961 ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
    22021 tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg cagagccaga
    22081 agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
    22141 tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc gcgcagcccg
    22201 tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc
    22261 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggcgacga
    22321 ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag aagcaccaga
    22381 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga cctcgctgga
    22441 ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt tcgctaaggc
    22501 gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
    22561 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
    22621 cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa
    22681 tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
    22741 taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt atgaccaggt
    22801 ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa ttgccgaaca
    22861 agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata accacgcact
    22921 agtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
    22981 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc
    23041 taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct ataacgtgca
    23101 tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct
    23161 gcatgtcgat gccgaagaag tactcacgct ggacggtttc tgcaaactgc gtcgtgaact
    23221 ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
    23281 aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg ccacctggac
    23341 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
    23401 aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg aacgtcgccg
    23461 tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct tcctgaaaga
    23521 taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc gtggcggcat
    23581 gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt tcttacacgc
    23641 tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta aaggtgaaac
    23701 ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca tggaaacccg
    23761 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct catggatggg
    23821 ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac tgactttcgt
    23881 ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa acaatgcgtt
    23941 tatccgccgt taatttcatg taagtcatgt tgttcctttt atttaaccgc ggactccgct
    24001 gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt gtacaacttt
    24061 ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgcttt gcgcttttct
    24121 accaccaaaa ccacagaacc tgatcgtaca ttgcagttat ctgccgaaca ggctgctcgc
    24181 atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt cgatgaaagc
    24241 gatctttctg agcatctggt aggacgaaaa atcaataaac gggaatgggc tggcaacgcg
    24301 tctgcatggc atgacacacc ggcggttgct cgtgatttat cacacgggct ttcctttgct
    24361 gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg ggggaatatc
    24421 caacgcttca atcggttatg tgaagattac acagggttga aagaacacga cgtcattggg
    24481 caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg cggcatccag gcgcaataac
    24541 cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc aacacgtaaa
    24601 ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg caaaaacgag
    24661 atttttttaa tctgttccgg taccgacatt accgaagagc gccgcactca ggagcgactg
    24721 cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc aatgcaggag
    24781 ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt gtatcttgat
    24841 ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga ccagttatta
    24901 cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt ggcgcgtcca
    24961 ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct ggaagcaatg
    25021 gcatcacgaa ttttaacccg cttacgcctc ccctttcgta ttggtttaat tgaagtttat
    25081 accagctgtt cagtaggtat ttcgctctct cccgaacatg gttcggacag cgcgactatt
    25141 attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg acaattttgc
    25201 gtttttactc cagaaatgaa tcagcgggta tttgaatatc tctggctgga taccaacttg
    25261 cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc acctagcgtg gcgaagtgcg
    25321 cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc caccgttgga
    25381 cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt gggtgattct
    25441 cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc gagtggcggt
    25501 aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga aacaggttct
    25561 ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga gttgtctgat
    25621 tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag gtgcgcaagt
    25681 gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc gctttccgat
    25741 cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac ctgtctcgca
    25801 gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg tgatcgccga
    25861 aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca atgagcggca
    25921 aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta
    25981 tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg tcattatata
    26041 tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta gcggcaattt
    26101 ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg aatttgatac
    26161 ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca
    26221 ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg ttgctgaatc
    26281 tacgccagac atcccgaaga caccgtggga gatttaacaa tcgtgccttc aggttttcct
    26341 atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt tataagaatt
    26401 acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca gggtcaggtt
    26461 agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg ccaggatctc
    26521 aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt ttcgcttgat
    26581 agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga actcgccgag
    26641 tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg cagcagcaat
    26701 gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat cacggtcagc
    26761 agctacatcg cgcatttgct gaaagacgcg ccttgtgaag tcattttgct cggtggcgtg
    26821 taccagaaaa aaagcgaaag tatggttggc cctttgacac gccagtgcat ccagcaggta
    26881 catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg attcaccggt
    26941 cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg aaaaagagtg cgaagcgata
    27001 gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg tcccgttgag
    27061 cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat gcatcttgag
    27121 cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc tacggatgat
    27181 ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta agactattta
    27241 cctgttatta acactctcaa gatataaaat tattatcagc gatataacag gaagtaatta
    27301 tcacgtgcgt gatataaccc tgcgcgcgag cagatttcac ggaataattt caccaggctt
    27361 attcttagct attatagtta tagagagctt acttccgtga atcataaatt caggagagag
    27421 tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta ctttggcaat
    27481 gtctctgagt gcctgttcta actggtctaa acgggaccgc aacaccgcaa tcggcgcggg
    27541 tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta cattaggtgg
    27601 tgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa taaatgtcga
    27661 ttatcgactc gcaattataa tacgctt
//
LOCUS       DADLZM010000041        27536 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLZM010000041 DADLZM010000000
VERSION     DADLZM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.041"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JNY73_001734"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JNY73_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY8047766.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JNY73_001735"
     CDS             complement(1614..1958)
                     /locus_tag="JNY73_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY8047767.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JNY73_001736"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JNY73_001736"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY8047768.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JNY73_001737"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JNY73_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY8047769.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDIYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JNY73_001738"
     CDS             complement(6144..6842)
                     /locus_tag="JNY73_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY8047770.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGVAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JNY73_001739"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JNY73_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY8047771.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JNY73_001740"
     CDS             7537..8574
                     /locus_tag="JNY73_001740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY8047772.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JNY73_001741"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JNY73_001741"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY8047773.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JNY73_001742"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JNY73_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY8047774.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JNY73_001743"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JNY73_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY8047775.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JNY73_001744"
     CDS             10745..11626
                     /locus_tag="JNY73_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8047776.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JNY73_001745"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JNY73_001745"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY8047777.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JNY73_001746"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JNY73_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY8047778.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JNY73_001747"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JNY73_001747"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY8047779.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JNY73_001748"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JNY73_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY8047780.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JNY73_001749"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JNY73_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY8047781.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JNY73_001750"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JNY73_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY8047782.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JNY73_001751"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JNY73_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY8047783.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHACLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JNY73_001752"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JNY73_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY8047784.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JNY73_001753"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JNY73_001753"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY8047785.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JNY73_001754"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JNY73_001754"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY8047786.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JNY73_001755"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JNY73_001755"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY8047787.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JNY73_001756"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JNY73_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY8047788.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JNY73_001757"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JNY73_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY8047789.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JNY73_001758"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JNY73_001758"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY8047790.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JNY73_001759"
     CDS             24893..25273
                     /locus_tag="JNY73_001759"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY8047791.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JNY73_001760"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JNY73_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY8047792.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JNY73_001761"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JNY73_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY8047793.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27536
                     /locus_tag="JNY73_001762"
     CDS             26442..>27536
                     /locus_tag="JNY73_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY8047794.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6791 a         6856 c         7220 g         6669 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaagcgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa atatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgcca ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag gcagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaattg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgatac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcatgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagt atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc actggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga aaacatacag cccggacggg gctgta
//
LOCUS       DADLZM010000042        27233 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLZM010000042 DADLZM010000000
VERSION     DADLZM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.042"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            14..4591
                     /locus_tag="JNY73_001763"
     CDS             14..4591
                     /locus_tag="JNY73_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY8047795.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDA
                     SVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANAD
                     YDNVGDALNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSD
                     AINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALL
                     WDKTANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQII
                     NQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAI
                     GQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDD
                     GKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAM
                     GAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGY
                     TAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSI
                     TNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIA
                     AADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVR
                     YDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQR
                     MVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSM
                     VSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            4959..6614
                     /gene="lldP"
                     /locus_tag="JNY73_001764"
     CDS             4959..6614
                     /gene="lldP"
                     /locus_tag="JNY73_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY8047796.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6614..7390
                     /gene="lldR"
                     /locus_tag="JNY73_001765"
     CDS             6614..7390
                     /gene="lldR"
                     /locus_tag="JNY73_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY8047797.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7387..8577
                     /gene="lldD"
                     /locus_tag="JNY73_001766"
     CDS             7387..8577
                     /gene="lldD"
                     /locus_tag="JNY73_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY8047798.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8773..9246
                     /gene="trmL"
                     /locus_tag="JNY73_001767"
     CDS             8773..9246
                     /gene="trmL"
                     /locus_tag="JNY73_001767"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY8047799.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9299..10120)
                     /gene="cysE"
                     /locus_tag="JNY73_001768"
     CDS             complement(9299..10120)
                     /gene="cysE"
                     /locus_tag="JNY73_001768"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY8047800.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10200..11219)
                     /gene="gpsA"
                     /locus_tag="JNY73_001769"
     CDS             complement(10200..11219)
                     /gene="gpsA"
                     /locus_tag="JNY73_001769"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY8047801.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11219..11686)
                     /gene="secB"
                     /locus_tag="JNY73_001770"
     CDS             complement(11219..11686)
                     /gene="secB"
                     /locus_tag="JNY73_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY8047802.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11749..12000)
                     /gene="grxC"
                     /locus_tag="JNY73_001771"
     CDS             complement(11749..12000)
                     /gene="grxC"
                     /locus_tag="JNY73_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY8047803.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12142..12573)
                     /locus_tag="JNY73_001772"
     CDS             complement(12142..12573)
                     /locus_tag="JNY73_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY8047804.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12818..14362
                     /gene="gpmM"
                     /locus_tag="JNY73_001773"
                     /gene_synonym="pgmI"
     CDS             12818..14362
                     /gene="gpmM"
                     /locus_tag="JNY73_001773"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY8047805.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14372..15655
                     /gene="envC"
                     /locus_tag="JNY73_001774"
     CDS             14372..15655
                     /gene="envC"
                     /locus_tag="JNY73_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY8047806.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15659..16618
                     /locus_tag="JNY73_001775"
     CDS             15659..16618
                     /locus_tag="JNY73_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY8047807.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16605..17639)
                     /gene="waaH"
                     /locus_tag="JNY73_001776"
     CDS             complement(16605..17639)
                     /gene="waaH"
                     /locus_tag="JNY73_001776"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY8047808.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17878..18903)
                     /gene="tdh"
                     /locus_tag="JNY73_001777"
     CDS             complement(17878..18903)
                     /gene="tdh"
                     /locus_tag="JNY73_001777"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY8047809.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18913..20109)
                     /gene="kbl"
                     /locus_tag="JNY73_001778"
     CDS             complement(18913..20109)
                     /gene="kbl"
                     /locus_tag="JNY73_001778"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY8047810.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20384..21241)
                     /gene="yibB"
                     /locus_tag="JNY73_001779"
     CDS             complement(20384..21241)
                     /gene="yibB"
                     /locus_tag="JNY73_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY8047811.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21546..22478
                     /gene="rfaD"
                     /locus_tag="JNY73_001780"
     CDS             21546..22478
                     /gene="rfaD"
                     /locus_tag="JNY73_001780"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY8047812.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22488..23534
                     /gene="rfaF"
                     /locus_tag="JNY73_001781"
     CDS             22488..23534
                     /gene="rfaF"
                     /locus_tag="JNY73_001781"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY8047813.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23538..24530
                     /gene="rfaC"
                     /locus_tag="JNY73_001782"
     CDS             23538..24530
                     /gene="rfaC"
                     /locus_tag="JNY73_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY8047814.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLDYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24527..25735
                     /gene="rfaL"
                     /locus_tag="JNY73_001783"
     CDS             24527..25735
                     /gene="rfaL"
                     /locus_tag="JNY73_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY8047815.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25771..26913)
                     /locus_tag="JNY73_001784"
     CDS             complement(25771..26913)
                     /locus_tag="JNY73_001784"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY8047816.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26922..>27233)
                     /locus_tag="JNY73_001785"
     CDS             complement(26922..>27233)
                     /locus_tag="JNY73_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY8047817.1"
                     /translation="KYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHDWA
                     NYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNVMK
                     G"
BASE COUNT         7001 a         6836 c         6733 g         6663 t
ORIGIN      
        1 gcgagcatca ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta
       61 tactgttacc agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt
      121 aatttctgcg ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg
      181 gaatgacaac ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc
      241 tataggcaaa ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt
      301 gggttatgac gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc
      361 gattggtggt gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat
      421 agcactgggt gcctcttcgt attcattggg ccaatactca atggccctgg ggcgtagcag
      481 tgtagctagc ggtactgaca gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa
      541 cgctattgcg ataggtgctg agaccgaagc cgctgaaaat gcaactgcta ttggcaataa
      601 tgcgaaggca aaagggacta atagcatggc aatggggttc ggaagccttg ccgataaagt
      661 caatactatc gcattaggaa atggcagcca ggctctggca gataatgcaa tcgccatagg
      721 ccagggcaac aaagctgatg gcgtggatgc catcgctctg ggtaatggta gccagtcgag
      781 aggcttaaac accattgcct taggcacagc cagtaatgca actggtgata agagtcttgc
      841 gcttggtagt aatagcagtg ccaacggtat taactctgtc gcgctgggcg cagattccat
      901 tgcggattta gacaataccg tctctgtcgg caatagttca ttaaaacgca agatcgttaa
      961 tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc attaatggtt cacagcttta
     1021 tgccattagc gactcggtag caaaaaggct tggaggaggg gctgcagtag atgttgatga
     1081 cggtactgtt acagcaccaa cctacaattt aaaaaatggt agcaaaaata acgtaggggc
     1141 tgcgctcgct gtacttgatg aaaacaccct gcaatgggac caaaccaaag gcaaatacag
     1201 cgctgctcat ggtactagta gcccaactgc cagcgtaatc accgatgttg cggatggcac
     1261 gatttcagcc tccagtaagg atgcggttaa cggttcccaa ctgaaagcta ccaatgacga
     1321 tgtcgaagcc aacaccacca atatcaccaa cctgacggat tccgttggtg accttcaggc
     1381 tgatgccctg ctctggaacg aaactaaaaa ggcattcagt gcagctcacg gccaggatac
     1441 caccagcaaa ataaccaacg ttaaagatgc cgacctgacg gctgacagca ctgatgctgt
     1501 taacggctct cagctgaaaa ccaccaacga tgctgtggcg acgaatacca ccaatatcgc
     1561 caataacact tccaatattg ccactaacac caccaacatc tctaacctga ctgagacggt
     1621 gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtat tcacggcagc
     1681 tcatggcacc gagaccacca gcaaaataac caacgttaaa gatggcgacc tgacgactgg
     1741 cagcaccgat gccgttaacg gctctcagct gaaaaccacc aacgatgccg tggcgacgaa
     1801 taccaccaat atcgccacta acaccaccaa catctctaat ctgactgaga cggtgactaa
     1861 tcttggtgag gatgcgctga aatgggataa ggacaatggt gtcttcactg cagctcatgg
     1921 caacaatacc gccagcaaaa tcaccaatat cctggacggc acagtcactg caaccagttc
     1981 cgatgccatt aacggtagcc agctttatga cttaagcagc aatatcgcca cctacttcgg
     2041 cggcaatgct tctgtgaata ctgacggcgt gtttaccggt ccaacctaca aaatcggtga
     2101 aacaaattat tataacgtcg gcgatgcact ggctgcgatt aactcctcat ttagcacgtc
     2161 tctcggcgat gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca aacacggtac
     2221 taatggtgac gcaagcgtga tcactgatgt cgcagatggt gaaatttcag actccagttc
     2281 tgacgcagta aacggctcac aactccacgg cgtgagcagt tatgttgttg atgcgctggg
     2341 gggtggtgcc gaagtcaatg cagacggcac catcactgcg ccgacgtaca ccattgctaa
     2401 tgctgattac gataatgtcg gtgatgccct gaatgctatc gataccactc ctgacgacgc
     2461 tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga
     2521 taaaactgcc agtgtaatca ctaacgtcgc taacggtgca atctctgctg ccagcagcga
     2581 cgcgattaac ggctcacaac tctataccac caataagtac atcgctgatg cgctgggtgg
     2641 tgacgcagaa gtcaacgctg acggcaccat caccgcaccg acttacacca ttgcgaacgc
     2701 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
     2761 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
     2821 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
     2881 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
     2941 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
     3001 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
     3061 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
     3121 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
     3181 aggttctcgt gacactagcg taacggaaaa tggcttggtg attggttatg acaccactga
     3241 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
     3301 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
     3361 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
     3421 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
     3481 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
     3541 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
     3601 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
     3661 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
     3721 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
     3781 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
     3841 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
     3901 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
     3961 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
     4021 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
     4081 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
     4141 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
     4201 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
     4261 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
     4321 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
     4381 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
     4441 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
     4501 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
     4561 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
     4621 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
     4681 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
     4741 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
     4801 aactcacatc aatattgcta atacataaca tttagttaac cattcattgt cattatccct
     4861 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
     4921 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
     4981 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
     5041 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
     5101 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
     5161 cggtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
     5221 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
     5281 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
     5341 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
     5401 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
     5461 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
     5521 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
     5581 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
     5641 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
     5701 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
     5761 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatataa
     5821 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
     5881 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
     5941 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
     6001 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
     6061 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
     6121 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
     6181 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
     6241 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
     6301 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
     6361 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
     6421 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
     6481 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
     6541 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
     6601 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
     6661 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
     6721 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
     6781 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
     6841 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
     6901 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
     6961 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
     7021 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
     7081 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
     7141 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
     7201 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
     7261 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
     7321 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
     7381 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
     7441 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
     7501 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
     7561 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
     7621 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
     7681 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
     7741 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
     7801 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
     7861 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
     7921 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
     7981 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
     8041 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
     8101 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
     8161 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
     8221 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
     8281 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
     8341 attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca
     8401 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
     8461 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
     8521 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
     8581 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
     8641 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
     8701 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
     8761 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
     8821 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
     8881 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
     8941 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
     9001 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
     9061 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
     9121 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
     9181 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
     9241 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
     9301 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
     9361 ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg
     9421 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
     9481 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
     9541 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
     9601 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
     9661 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
     9721 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
     9781 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
     9841 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
     9901 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
     9961 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    10021 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    10081 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    10141 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    10201 tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    10261 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    10321 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    10381 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg
    10441 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    10501 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    10561 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    10621 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    10681 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    10741 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    10801 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    10861 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    10921 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    10981 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    11041 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    11101 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    11161 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    11221 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    11281 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    11341 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    11401 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    11461 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    11521 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    11581 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    11641 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    11701 taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag
    11761 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    11821 tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg
    11881 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    11941 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    12001 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    12061 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    12121 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    12181 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    12241 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    12301 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    12361 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    12421 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    12481 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    12541 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc aacgtagtga
    12601 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    12661 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    12721 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    12781 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    12841 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    12901 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    12961 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    13021 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    13081 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    13141 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    13201 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    13261 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    13321 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    13381 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    13441 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    13501 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    13561 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    13621 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    13681 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    13741 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    13801 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    13861 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    13921 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    13981 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    14041 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    14101 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    14161 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    14221 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    14281 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    14341 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    14401 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    14461 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    14521 tattcaggcc gatatcgccg caaaagagca cgcggtacgc caaaagcaac aacaacgcgc
    14581 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    14641 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    14701 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    14761 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    14821 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    14881 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    14941 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    15001 tcaggttctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    15061 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    15121 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    15181 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    15241 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    15301 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    15361 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    15421 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    15481 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    15541 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    15601 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    15661 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    15721 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    15781 ccaagtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    15841 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    15901 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    15961 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    16021 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    16081 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    16141 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    16201 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    16261 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    16321 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    16381 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    16441 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    16501 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    16561 gtggcaaggc tggggcaaac agcacgaagc ggcgaagttt aacgctagcg caaattaaag
    16621 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    16681 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    16741 tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat atccggctct
    16801 ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg aaattccgga
    16861 taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg ggtaatctta
    16921 atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg actcactgac
    16981 gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg cagcgcgtta
    17041 aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat aaatttaatg
    17101 ttatttttaa caataacatc acggcgataa acccccatcc agacaacgtg agtccaacgg
    17161 cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacaccggt tgagcgaagg
    17221 cgatcgctgg ggatggattg ccaggtttct cccgtttcac gaaaagacca gtcagcgttg
    17281 cactgcgcca cgtcgaggtc gtcctctaac gccatggtca tcagcgtttc gtacatggtg
    17341 ggatagacct catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc cacctcgatc
    17401 ccacgattac gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg aacgtgcgga
    17461 tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc atcgttaata
    17521 ataatgattt ccagagcagt ccaggtttgc gtaattaaag attccataca agtgcggaaa
    17581 tcatcgcccg cattatataa cggaataata acactaagtt tattggtgct gttcatcata
    17641 aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt aagaaactat
    17701 taagcctgtg agatattatt tttacaggtg agagagtcac ttcagggtag agagataatg
    17761 agaacagcta tataaaagat cagcgcaatt acgaactctt tattacactg acaggatttt
    17821 acataattaa cagattatcc tcagataaaa gggctggaat accagccctt gttcgtgtta
    17881 atcccagctc agaataactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
    17941 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat
    18001 cagcgccgcc atcttgtacc aagtttcaaa catctcacga ccgtaaatgc ctttaatgaa
    18061 caagccttta aagatcactt tggtccagtc gatagacata tcagacggtg gaatacccag
    18121 catcgcaata cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc
    18181 gccggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc
    18241 attaaggttt tctttcgcaa cgttaaccgc acgggtgata cccattttgc gcgccagctc
    18301 aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac
    18361 cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag
    18421 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
    18481 gtcggagata ttgtcgggga ttttgaaagc gttgaacgcc ggaatcacca gatattcggc
    18541 gaagcagccc ggacggttaa caccgacacc aatggtgttg cggcacagat gggtacgacc
    18601 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
    18661 gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
    18721 cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
    18781 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
    18841 cggtacaggg acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
    18901 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
    18961 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
    19021 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
    19081 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
    19141 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
    19201 ccacagacgg tcacgcaact cgctacccgc ttcaaccatc tccagcactt tgatggaagc
    19261 ggcgacaatc gctggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
    19321 ccactcaacc acttctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
    19381 accgagcgtg ccggtgatga tgtcgacacg ccccatcaca tcgcagtatt catgggaacc
    19441 acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc
    19501 gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa
    19561 cacgccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
    19621 agcttcaagc tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg
    19681 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc
    19741 cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
    19801 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
    19861 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
    19921 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
    19981 gtggcttcca tcagccacgg tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
    20041 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
    20101 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatag
    20161 gtcgcgacga gatgatgcgt taacacttct gcaaaattca ggataaataa cgtgtatctt
    20221 gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg agcataatga
    20281 tgagtgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct
    20341 atctcagttg gcatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga
    20401 aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat
    20461 cgaaccattt tcctctcccc agatagttta ggttgaagag atcggaacgt ttgtaatagc
    20521 acatgacaaa aatgccctgg tcatcgtcca caatgttatt gtttagtgtt attttttgac
    20581 tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc
    20641 caataatata tacatgattg ccaatcatga aatcaaaagc ctgttgttga gatgtaacag
    20701 taagcccttt ttttatagtg aaaagatgca tcttattttc atcaaatggg aaatcccaaa
    20761 ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa
    20821 gaggcgtttt cactaaaccc atgttgatag ctttatttac aaaataggcc ttaaggttac
    20881 ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc
    20941 tatttgtaaa cgactcatct ttttgaattt tttcgatacg acttctgata tatctaaatt
    21001 tcttttttat atcgataacg ataaccgttg ttggttttcc gttgcgaatc gcttcaatcc
    21061 ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct
    21121 caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag
    21181 cagtccaatc ccctctacca atgtcaaaat atgcagtgat aattgtggtg gatgatttca
    21241 ttactaataa tgaccattaa tataaaaaaa gaattatatc ataaatatta acagaatgta
    21301 tactgtatat tatccattca aatgattccg ttatttatca tacatcggaa tattgatact
    21361 aaagcactat tcacatgcaa aaccaacatc cgccatgaag gactagctaa aacccaaact
    21421 agtttgttgc aattagcatc cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg
    21481 atggtatgat tacagacatt cgtgtctgag attgtctctg actccataat tcgaaggtta
    21541 cagttatgat catcgttacc ggcggcgcgg gctttatcgg cagcaacatc gttaaggccc
    21601 tgaatgataa aggcatcacc gatattctgg tggtggacaa cctgaaagac ggcaccaagt
    21661 ttgtgaacct ggtggatctg gatatcgcgg actatatgga taaggaagac ttcctgatcc
    21721 agattatggc tggcgaagag ttcggtgatg tcgaagcgat tttccacgaa ggcgcatgct
    21781 cttccaccac cgagtgggat ggcaagtata tgatggataa caactatcaa tactccaaag
    21841 agctgctgca ctactgtctg gagcgcgaaa tcccgttcct gtatgcctct tccgcagcca
    21901 cctacggcgg acgcacctcc gactttattg aatcccgcga gtacgaaaaa ccgttgaatg
    21961 tctacggtta ctcaaaattc ctgtttgatg aatatgttcg tcaaatcctg ccagaagcga
    22021 actcgcagat tgttggcttc cgctatttca acgtttatgg accgcgtgaa ggccataaag
    22081 gcagcatggc gagcgtcgct ttccatctca acacccagct taataacggt gaatcaccga
    22141 agctgttcga aggtagcgag aacttcaaac gtgacttcgt ctatgtgggc gacgtggcag
    22201 atgtaaacct gtggttcctg gaaaatggcg tttccggtat cttcaacctc ggcaccggtc
    22261 gtgcagaatc cttccaggca gtagcagatg ctacgctggc ttatcacaag aaaggccaga
    22321 tcgaatacat tccgttcccg gataagctga aaggccgcta ccaggcgttc actcaggcag
    22381 atctgacaaa tctgcgcgcg gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg
    22441 taacggaata catggcctgg ctgaatcgcg acgcataaga gctctgcatg aaaatactgg
    22501 tgatcggccc gtcttgggtt ggcgacatga tgatgtcgca aagtctctat cgcacgctcc
    22561 aggcgcgcta tccccaggcg ataatcgatg tgatggcacc ggcatggtgc cgtccattat
    22621 tatcgcggat gccggaagtt aacgaagcta tccctatgcc tctcggtcac ggagcgctgg
    22681 aaatcggcga acgccgcaaa ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct
    22741 acgtcttacc aaactccttc aaatctgcat tagtgccttt cttcgcgggt attcctcatc
    22801 gcaccggctg gcgcggcgag atgcgctacg gtttactcaa cgatgtacgc gtgctcgata
    22861 aagaagcctg gccgctaatg gtggaacgct atgtcgcgct ggcctatgac aaaggcatta
    22921 tgcgtaccgc acaagatctg ccgcagccat tgttatggcc gcagttgcag gtgagcgaag
    22981 gtgaaaaatc atatacctgt aatcaatttt cgctttcatc agaacgtccg atgattggct
    23041 tttgcccggg tgcggagttt ggtccggcaa aacgctggcc acactaccac tatgcggaac
    23101 tggcaaagca gctgattgat gaaggttatc aggtggttct gtttggctct gcgaaagatc
    23161 atgaagcggg caatgagatt cttgccgctt tgaataccga gcagcaggca tggtgtcgga
    23221 acctggcggg ggaaacacag cttgatcaag cggttatcct gattgcagcc tgtaaagcca
    23281 ttgtcactaa cgattctggc ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg
    23341 ccctgtatgg tccaagtagc ccggacttca caccgccgct atcccataaa gcgcgcgtga
    23401 tccgtctgat taccggctat cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc
    23461 agagcttgat cgacattact ccccagcgcg tactggaaga actcaacgcg ctattgttac
    23521 aagaggaagc ctgacggatg cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc
    23581 tccatacgtt gcccgcactc actgatgccc agcaggcaat cccagggatt aagtttgact
    23641 gggtggtgga agaagggttc gcacagattc cttcctggca cgctgccgtt gagcgagtta
    23701 ttcctgtggc aatacgtcgc tggcgtaaag cctggttctc ggcccccata aaagctgaac
    23761 gcaaagcgtt tcgtgaagcg ctacaagcag agaactatga cgcagttatc gacgctcagg
    23821 ggctggtaaa aagcgcggca ctggtgacac gtctggcgca tggcgtaaag catggattgg
    23881 actggcaaac cgctcgcgaa cctttagcca gcctgtttta caattgtaag catcatattg
    23941 caaaacagca gcacgccgta gaacgcaccc gcgaactgtt tgccaaaagt ttggactata
    24001 gcaaaccgca aacccagggc gattatgcta tcgcacagca ttttctgacg aacctgccta
    24061 cagatgctgg cgaatatgcc gtatttcttc atgcgacgac ccgtgatgat aaacactggc
    24121 cggaagaaca ctggcgagaa ttgattggtt tactggctga ttcaggaata cggattaaac
    24181 ttccgtgggg cgcgccgcat gaggaagaac gggcgaaacg actggcggaa ggatttgctt
    24241 atgttgaagt attgccgaag atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta
    24301 aatttgtagt gtcggtggat acggggttaa gccatttaac ggcggcactg gatagaccca
    24361 atatcacggt ttatggacca accgatccgg gattaattgg tgggtatggg aagaatcaag
    24421 tagagtgtcg ttctacaagt atgtctcttg cagatttgcc agctcaaacc gtttttcaaa
    24481 atttaaatct tgaaataata accaataagt tgacatcgga gataagatga cctcaacatt
    24541 atttttctct ctcgagaaaa aaaactggat agcgtactgg aacagagctc tcgtattctt
    24601 attcattacc acctattttt tgggtgggat tacaaggtat aaacatctta ttgttattct
    24661 tatgacaata acgacaatcg tctatctctg caaacggcca aaacactatc tctcactatt
    24721 taaaacattt ctttttggta gtgttgccat attaactatt gctgcattgc tgtcacttct
    24781 tcaatcccct gatgcaggtg ctagcatgaa ggaaattttc aaagctatta ttgagaatac
    24841 tttactatgc acaatagcaa taccggtcat attgagagac gagaaaagag aagatgtcga
    24901 aaaaatcgtt ttcttctcat ttattagtgc gttgggctta cgctgttttt ctgaattgat
    24961 tacctattat aaggactatc aacaagggat aatgccattc gcagattata gacaccgtag
    25021 catttctgac tcgatggtct ttttattccc tgcattgtta aatctctggc ttatcaaatc
    25081 agcaaaatac cgcatttctt ttgtggttct aagcgttatt tttatttttc tgatattagg
    25141 aactttatcc agaggggcct ggctttccgt gttagtcatt ggattaatat ggattctgat
    25201 gtttaaacaa tggaagttac tattagtagg agtaatggtt tccatcattg cattgtcggt
    25261 tattttcaca cataaggaga tgaccgcaaa gctaacgtat aaacttcaac aaactaatag
    25321 ttcttatcgc tatgcaaatg gtactcaagg cagcgcactc gatctaatat tagaaaatcc
    25381 tgttattggt tatggttacg gtaacgttgc atataaagat gtctataata aacgtgtcat
    25441 tgattatcca gaatggacct ttagacaatc aatagggcca cataattttg cgctattcat
    25501 ctggtttggc actggtttat tagggctggt aagtcttatg atgctatact gtgcaatatt
    25561 gaaagagtgt ataaaaaatg gcgtcaagaa taaatatcgc tcaccatata atgcatatta
    25621 tataatctta ctatctttta taggttattt tgttatccgt ggaaacgtag aacaaattga
    25681 accaaattta ttaggcgttt acgccggctt attattagcg atgaaaaaca agtaaaaaat
    25741 aaaaaaggct gcataatgcg gcctttttat ttattcaaac caattatgaa taacctcttc
    25801 gaatctttga gttacgccat cccaactgta gtgatcaaac acaaagtcct gaccttgctt
    25861 tgcaactgct gttaattcag gattcgctaa cgtttttaaa atatcgctgc ttatggagtc
    25921 tgcagtcata ggttctttta aatggaatcc cgtagtattt tctttgacaa attccgtcat
    25981 ccccccacgc gtactcacta aaacaggctt accagcaccc atagcttcaa ttgccaccat
    26041 gcaaaatggt tcctggaact gggaaggaat gacgactaaa tctgccagtg ggtaataaca
    26101 atgcatcttt tcaggcggta tgcttcctaa cataatgcaa cgatccttca aacgttttgc
    26161 aatttccctg acattgcgtt gatatgcacc tttgtcactt ttactcattt ctgtataatc
    26221 accaatcaca actaatttta aatttttgtg agcagcggcc aatttttcga atgattgcat
    26281 caacagcaat atgcctttgt cagggacgat acgcccagca taaaaaataa ttttctcttc
    26341 aggggagata ttaagttcgc ttctagtgat cggctgaaaa ttagattgat aggtttctaa
    26401 atcaattcca tttggtacaa tctcaatatc tgcatttgct aaataagatt ggtaatactt
    26461 tttcaagtat aaactaggga ctatcatttt aacattttgc tcaagcaatt ttggttcaaa
    26521 tgcattatgc atatgtataa ccactcttgc ctgaggagct cgcttacgaa tttgagtgta
    26581 tagcttcatg ctattatgaa ctacaataac actttctttc gttataggaa aatcatgtgc
    26641 aatattcaat attctctgtg aatatggaag aggatcaagt cttgtccatt tttgaaataa
    26701 ccgcttatat atgcgactga atccaaccct atgaatacta caattatcat taacaaaggt
    26761 gtaatttgag tatcctggat ttttgataca aacaatacga tttggaaaat tggtacgctg
    26821 agcaacctga tacatccacg tttcaacagc agccgctccg cgaggaggaa ttgaaaaaat
    26881 aggagtaacc gtaaatatta ttttatcaac catctcgtat attaaccttt cataacatta
    26941 tatttaattg ctgtaaaaac accatcgaga aattttttct ggtaaagcaa gtgtttatat
    27001 ttttctttgt gctcatgttt tttaacagct tttttataag gtatatttct ccatggtgat
    27061 atattataaa tattacgaaa ataatctgca gaggcgtagt tcgcccaatc atgccatggc
    27121 ttagttatac cagtataatg tatgaagaca gtgtcatcat taataaaccg ggtgtaatat
    27181 tcactatttt tttcttcaaa ttctgacttt atagtataaa tacaattata ttt
//
LOCUS       DADLZM010000043        26934 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLZM010000043 DADLZM010000000
VERSION     DADLZM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.043"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..438
                     /locus_tag="JNY73_001786"
     CDS             <1..438
                     /locus_tag="JNY73_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY8047818.1"
                     /translation="LIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQ
                     VELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAW
                     FTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(500..1120)
                     /gene="rhtB"
                     /locus_tag="JNY73_001787"
     CDS             complement(500..1120)
                     /gene="rhtB"
                     /locus_tag="JNY73_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY8047819.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1231..2253
                     /gene="pldB"
                     /locus_tag="JNY73_001788"
     CDS             1231..2253
                     /gene="pldB"
                     /locus_tag="JNY73_001788"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY8047820.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWHQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2261..3061
                     /gene="yigL"
                     /locus_tag="JNY73_001789"
     CDS             2261..3061
                     /gene="yigL"
                     /locus_tag="JNY73_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY8047821.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3137..4036
                     /gene="bioP"
                     /locus_tag="JNY73_001790"
     CDS             3137..4036
                     /gene="bioP"
                     /locus_tag="JNY73_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY8047822.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3924..4877)
                     /gene="metR"
                     /locus_tag="JNY73_001791"
     CDS             complement(3924..4877)
                     /gene="metR"
                     /locus_tag="JNY73_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY8047823.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5113..7374
                     /gene="metE"
                     /locus_tag="JNY73_001792"
     CDS             5113..7374
                     /gene="metE"
                     /locus_tag="JNY73_001792"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY8047824.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7413..8228)
                     /locus_tag="JNY73_001793"
     CDS             complement(7413..8228)
                     /locus_tag="JNY73_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY8047825.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8490..9251
                     /gene="udp"
                     /locus_tag="JNY73_001794"
     CDS             8490..9251
                     /gene="udp"
                     /locus_tag="JNY73_001794"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY8047826.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9392..10819
                     /gene="rmuC"
                     /locus_tag="JNY73_001795"
     CDS             9392..10819
                     /gene="rmuC"
                     /locus_tag="JNY73_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY8047827.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSR"
     gene            10914..11669
                     /gene="ubiE"
                     /locus_tag="JNY73_001796"
     CDS             10914..11669
                     /gene="ubiE"
                     /locus_tag="JNY73_001796"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY8047828.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11683..12288
                     /gene="ubiJ"
                     /locus_tag="JNY73_001797"
     CDS             11683..12288
                     /gene="ubiJ"
                     /locus_tag="JNY73_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY8047829.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12285..13925
                     /gene="ubiB"
                     /locus_tag="JNY73_001798"
     CDS             12285..13925
                     /gene="ubiB"
                     /locus_tag="JNY73_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY8047830.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHRVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14004..14273
                     /gene="tatA"
                     /locus_tag="JNY73_001799"
     CDS             14004..14273
                     /gene="tatA"
                     /locus_tag="JNY73_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY8047831.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            14277..14792
                     /gene="tatB"
                     /locus_tag="JNY73_001800"
     CDS             14277..14792
                     /gene="tatB"
                     /locus_tag="JNY73_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY8047832.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14795..15571
                     /gene="tatC"
                     /locus_tag="JNY73_001801"
     CDS             14795..15571
                     /gene="tatC"
                     /locus_tag="JNY73_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY8047833.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15613..16395
                     /gene="tatD"
                     /locus_tag="JNY73_001802"
     CDS             15613..16395
                     /gene="tatD"
                     /locus_tag="JNY73_001802"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY8047834.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16392..16880)
                     /gene="rfaH"
                     /locus_tag="JNY73_001803"
     CDS             complement(16392..16880)
                     /gene="rfaH"
                     /locus_tag="JNY73_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY8047835.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17047..18540
                     /gene="ubiD"
                     /locus_tag="JNY73_001804"
     CDS             17047..18540
                     /gene="ubiD"
                     /locus_tag="JNY73_001804"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY8047836.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVVTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18586..19287
                     /gene="fre"
                     /locus_tag="JNY73_001805"
     CDS             18586..19287
                     /gene="fre"
                     /locus_tag="JNY73_001805"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY8047837.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19380..20543)
                     /gene="fadA"
                     /locus_tag="JNY73_001806"
     CDS             complement(19380..20543)
                     /gene="fadA"
                     /locus_tag="JNY73_001806"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY8047838.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20553..22742)
                     /gene="fadB"
                     /locus_tag="JNY73_001807"
     CDS             complement(20553..22742)
                     /gene="fadB"
                     /locus_tag="JNY73_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY8047839.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22932..24263
                     /gene="pepQ"
                     /locus_tag="JNY73_001808"
     CDS             22932..24263
                     /gene="pepQ"
                     /locus_tag="JNY73_001808"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY8047840.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24263..24877
                     /locus_tag="JNY73_001809"
     CDS             24263..24877
                     /locus_tag="JNY73_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY8047841.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24916..26367
                     /gene="trkH"
                     /locus_tag="JNY73_001810"
     CDS             24916..26367
                     /gene="trkH"
                     /locus_tag="JNY73_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY8047842.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26379..26924
                     /gene="hemG"
                     /locus_tag="JNY73_001811"
     CDS             26379..26924
                     /gene="hemG"
                     /locus_tag="JNY73_001811"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY8047843.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6166 a         6858 c         7431 g         6479 t
ORIGIN      
        1 ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggtgggcgg tggcctgtat
       61 ctctgctgga tgggttacca gatgctacgc ggtgcactga aaaaagaggc tgtatctgcg
      121 cctgcaccac aggttgagct ggcgaaaagc gggcgcagtt tcctgaaagg cttactgacc
      181 aatctcgcca atccgaaagc gattatctat tttggttcgg tattctcatt gtttgtcggc
      241 gataacgttg gcactaccga gcgctggggc attttcgcgt tgattattgt cgaaacactg
      301 gcgtggttta ccgtcgttgc cagtctgttt gccctgccgc aaatgcgccg tggttatcaa
      361 cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt tggcattcat
      421 ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa ggcgtttacg
      481 ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct cccaccagca
      541 taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct ttaatccata
      601 gagcaatcct ttgagcaagg gtggcgtaac cgatcatcac aataatatcg accacaatag
      661 tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg atgaattgcg
      721 gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca aaaactgcgc
      781 gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaagatca attgcgccag
      841 cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc cactttaaca
      901 cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca accagcacaa
      961 tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg cgataaccgt
     1021 ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg cttaaaatga
     1081 tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc cggtgtgtct
     1141 atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgctt aaaaaaacga
     1201 ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga ctgggaaaca
     1261 agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg gcgtcagcgt
     1321 gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg ttttcgcgca
     1381 cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta cgtaaaatat
     1441 gcggaactgg cctacgacct gttccatttg gggtttgatg tcttaatcat cgaccatcgc
     1501 gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt taatcgcttt
     1561 aatgattatg ttgatgatct ggcggcattc tggcatcagg aggttcagcc cggtccgtgg
     1621 cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt atttctgcaa
     1681 cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg gatcgtgatt
     1741 cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca tccacgtttc
     1801 cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc tatcaacgta
     1861 ctgacccaca gcagacagcg atatcgacgt aacttacgct tctatgctga tgacccaacg
     1921 attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc tggcgaacag
     1981 gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttacaagc tgaagaggaa
     2041 cgcgtggtgg ataaccgcat gcacgaccgt ttttgtgaac tccgcaccgc cgcgggccat
     2101 cctgtcgaag gaggacggcc gttggtaata aaaggtgctt accatgagat cctttttgaa
     2161 aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg attttttcaa caggcataac
     2221 tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg ttgttgcgtc
     2281 tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg ccaaagaaac
     2341 tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc gtcaccacgt
     2401 tgatgtgggg caaattcgcg ataatctgga gattaagtct tacatgatta cctccaatgg
     2461 tgcgcgcgtt cacgatctgg atggcaatct gatttttgct cataacctgg atcgcgacat
     2521 cgccagcgat ctgtttggcg tagtcaacga caatccggac atcattacta acgtttatcg
     2581 cgacgacgaa tggtttatga atcgccatcg cccggaagag atgcgctttt ttaaagaagc
     2641 ggtgttcaaa tatgcgctgt atgagccggg attactggag ccggaaggcg tcagcaaagt
     2701 gtttttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga ttaacgctcg
     2761 ctggggcgat cgcgtcaacg tcagtttctc taccttaacc tgtctggaag tgatggcggg
     2821 cggcgtgtcg aaaggccatg cgctggaagc agtggcgaag aaactgggct acagcctgaa
     2881 ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga tggcggggaa
     2941 aggctgcatt atgggcagtg cgcaccagcg tctgaaagat ctgcatccgg agctggaagt
     3001 tattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct atttatcgta
     3061 atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat actcgttaat
     3121 caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg ccttctcctt
     3181 tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg tgctggtgcg
     3241 cgttggcttg gcggcactcg tttttctgcc gtttctgcgt acccgtagca atagcctgaa
     3301 aacggtcggc ctgtatatgc tggtgggcgc gatgcagctc ggcgtgatgt atatgctgag
     3361 tttccgcgct tatctctacc tgacggtctc cgaattgttg ctgttcaccg tactgacgcc
     3421 gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct ggggctatgc
     3481 ttttagcgcc ttgctggcgg tgattggtgc cgggattatt cgctatgatc aggtcaccga
     3541 ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg ccatcggcat
     3601 ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg cctttgcgtg
     3661 gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca
     3721 gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg tggtggcttc
     3781 cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg gaacgctggg
     3841 cattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta tctggcacca
     3901 acagccgcac tggccaacgt ttattacagg cgcgctggtg atcctggcct cactgtgggt
     3961 gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca ggcgtgattg
     4021 cgcgctgagc gaataaacgc ttccgtaact ggctgacgct gctcgccatc gcgcacggcg
     4081 gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag accctggcgc
     4141 tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc ggcaaccatt
     4201 tgaatcaata ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc tgccggctga
     4261 agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaattaa tagcgtctcg
     4321 ctggcgagat cttccggtgt aatacgcgtt tttgccgcca gtggatggtc aggtgctaac
     4381 accaggcgta cttcataatc gaacatcggc gaataatgca ggccactgcg cggcaggata
     4441 tcggaggtca tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg gtcaaacgtc
     4501 acgcccgatt taaaatccat ctctacctgc ggccagttct tatggaaatt ttctaaagcg
     4561 ggtgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg cgtctgctgc
     4621 ggttcattgc aggcttgcag tgcctggctg atttgcggca gcacctggtt tgccagttgc
     4681 aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac aaatagccgg
     4741 aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg cgtctgatgc
     4801 aacgttgccg cagcggctgc gagcgagccg cagttccgca acgcttgtag cgttttcagg
     4861 tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg cttgaggaat
     4921 atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaaatc cttcgtcttt
     4981 taaatttatg gtgcgttggc tgcgtttctc caccccggtc acttacttca gtaagctccc
     5041 ggcgatgaat aaacttgccg ccttccctaa attcaaaatc cataggattt acatataatt
     5101 agaggaagaa aaatgacaat attgaatcac accctcggtt tccctcgcgt tggcctgcgt
     5161 cgcgagctga aaaaagtgca agaaagttat tgggcgggga actccacgcg tgaagaactg
     5221 ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca agcgggtatc
     5281 gacctgctgc cggtgggcga ttttgcctgg tacgatcatg tactgaccac cagtttgctg
     5341 ctgggtaatg ttccgccacg tcatcagaac aaagacggtt cggtagatat cgacaccctg
     5401 ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc
     5461 aaatggttta acaccaacta tcactacatg gtgccggagt tcgttaaagg ccaacagttc
     5521 aaactgacct ggacgcagct gctggaggaa gtggacgagg cgctggcgct gagccacaac
     5581 gttaagcctg tgctgctggg gccggttact tacctgtggc tggggaaagt gaaaggtgaa
     5641 cagtttgacc gccttagcct gctgaacgac attctgccgg tttatcagca agtgctggca
     5701 gaactggcga aacgcggcat cgagtgggta cagattgatg aacccgcgct ggtactggaa
     5761 ctaccacagg cgtggctgga cgcatacaaa cccgcttacg acgcgctcca gggacaggtg
     5821 aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa atctcgacac gattactgcg
     5881 ctgcctgttc aggggctgca tgtggacctt gtacatggta aagatgacgt tgttgaactg
     5941 cacaagcgcc tgccttctga ctggctgctg tctgcgggtc tgatcaatgg tcgtaacgtc
     6001 tggcgcgccg atcttactga gaaatatgcg caaattaagg acattgtcgg caaacgtgat
     6061 ttgtgggtgg catcttcctg ctcgttgctg cacagcccca tcgacctgag cgtggaaacg
     6121 cgtcttgatg cagaagtgaa aagctggttt gccttcgccc tacaaaaatg ccatgaactg
     6181 gcactgctgc gcgatgcgct gaacagtggt gacacggcag ctctggcaga gtggagcgcc
     6241 ccaattcagg cgcgtcgtca ctctactcgt gtacataatc cggcggtaga aaagcgtctg
     6301 gcggcgatca ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg tgctgaagcc
     6361 cagcgtgcgc gttttaaact gccagcgtgg ccgaccacca cgattggttc cttcccgcaa
     6421 accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga cgccaacaac
     6481 taccgcacgg gcattgcgga acatatcaag caggccattg ttgagcagga acgtttggga
     6541 ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca tggtggaata ctttggcgag
     6601 cacctcgacg gatttgtctt tacgcaaaac ggttgggtac agagctacgg ttcccgctgc
     6661 gtgaagccac cgattgtcat tggtgacatt agccgcccgg caccgattac cgtggagtgg
     6721 gcgaagtatg cgcaatcgct gaccgacaaa ccggtgaaag ggatgctgac ggggccggtg
     6781 accatactct gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacag
     6841 attgcgctgg cgctgcgtga tgaagtggcc gatctggaag ccgctggaat tggcatcatc
     6901 cagattgacg aaccggcgct gcgcgaaggt ttaccgctgc gtcgtagcga ctgggatgcg
     6961 tatctccagt ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa agatgacaca
     7021 caaatccaca ctcacatgtg ttattgcgag ttcaacgaca tcatggattc gattgcggcg
     7081 ctggacgcag acgtcatcac catcgaaacc tcgcgttccg acatggagtt gctggagtcg
     7141 tttgaagagt ttgattatcc aaatgaaatc ggtcctggcg tctatgacat tcactcgcca
     7201 aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga aagcggcaaa acgcattccg
     7261 gcagagcgcc tgtgggtcaa cccggactgt ggcctgaaaa cgcgcggctg gccagaaacc
     7321 cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt tgcgtcgggg gtaaatccaa
     7381 accgggtggt aataccaccc ggtcttttct cattacagcg acttcttccc accatactgc
     7441 ttaaaccatt ccagcatacg ctgccagcca tctttcgcag attcggcatg atagctcggg
     7501 cgataatcag cgttgaatgc atgcccggca tccgggtaca cgataatctc tgctttcgca
     7561 ttagcagccc gcagcgcctg gcgcatcgtt tcaacgctct cctgcggaat gctgttatcc
     7621 tgaccaccat ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc aacaggttgt
     7681 ttcggtgaat tcagcgactt gtcgcccgtc agtttgccgt accacgccac tgcggctttt
     7741 agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca gaatccggtg
     7801 atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcgac atgatcgaga
     7861 tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa cgtggggata
     7921 tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat agccagatac
     7981 ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc
     8041 tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggtctggc atggtaagca
     8101 ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc gtcgtccggg
     8161 gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt
     8221 gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac tatagtcagc
     8281 atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc agaatgaaga
     8341 gtgatttatg tgatatgaat cacttttggt gggtaaattt atgcaacgca tttgcgtcat
     8401 ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt tgcttcttct
     8461 gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt catctcggcc
     8521 tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg
     8581 tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac cgcgaattca
     8641 ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg
     8701 gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc accttcctgc
     8761 gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc ctggttacca
     8821 cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg
     8881 tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatctatt ggcgcgacaa
     8941 ctcacgttgg cgtgacagtt tcttctgata ccttctaccc aggtcaggaa cgttacgata
     9001 cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg caggcgatgg
     9061 gcgtaatgaa ctatgaaatg gaatctgcaa ctctgctgac catgtgtgca agtcagggcc
     9121 tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag atcccgaatg
     9181 ctgagacgat gaaacaaact gaaagccatg cggtgaaaat cgtggtggaa gcggcgcgtc
     9241 gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg
     9301 cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca attctatttt
     9361 gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg tttacgcagt
     9421 tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac atgcgcagca
     9481 aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca
     9541 acaaattacc caaagcgagc actggcgtgc agagtgtgaa ttgctcaata acgaagtgcg
     9601 cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa ccacgcggat
     9661 ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca gcgagcagcg
     9721 cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca atcgccgggt
     9781 tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg aacaactgga
     9841 cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac gccataccct
     9901 gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg aagcgatcaa
     9961 cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg aggtagtatt
    10021 gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag
    10081 catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc tgccgcaggg
    10141 aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct attttaacgc
    10201 cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg tgcgtaacca
    10261 tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa ctctggatta
    10321 cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc gccagccgga
    10381 gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta cgctgctggt
    10441 ggcgttgcgt actattgcca acctgtggcg ttatgagcat caaagccgta acgcccagca
    10501 aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg atgacatgtc
    10561 cgcgattggt caaagtctcg acaaagcgca ggataactac cgccaggcaa tgaaaaaact
    10621 ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt taggagtaga
    10681 aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccagg atgaagagta
    10741 tcgacttcgg tcggttccgg ggcagccgaa tgatgaagct tatcaacgcg atgatgaata
    10801 taatcagcag tcgcgctagc ccattagggg tagttaagcc gggtagaaat ctagggcatc
    10861 gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt gagatggtgg
    10921 ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg
    10981 atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg aatgatttga
    11041 tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc
    11101 gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg aaattctccc
    11161 gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa
    11221 tgggacgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat gttcaggcga
    11281 acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt tcgtttggtc
    11341 tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg
    11401 gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg agcaaagcct
    11461 atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg aacgacgccg
    11521 acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat accctgaaag
    11581 ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg gcaggggttg
    11641 tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt taaaccttta
    11701 gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc cgcgctgaaa
    11761 acagcccgct cgcgtctgct gggtaaagta ttgcgcgtgg aggtaaaagg cttttcgacg
    11821 tcattgattc tggtgttcag cgaacgccag gttgatgtac tgggcgaatg ggcaggcgat
    11881 gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga tcgccagcag
    11941 cttaccgcac tgattcgcag tggtgagctg gaagtgcagg gcgatattca ggtggtgcaa
    12001 aacttcgttg cgctggcaga tctggcagag ttcgaccctg cggaactgct ggccccttat
    12061 accggtgata tcgccgctga aggaatcagc aaagccatgc gcggaggcgc aaagttcctg
    12121 catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga agagtggcgt
    12181 atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc cgtcgagcgt
    12241 gctgttgatg ccctgaccaa acggctggaa aaactggagg ctaaatgacg cctggtgaag
    12301 tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat gaactgatcc
    12361 ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc tggatgccaa
    12421 atcggcataa agacaaactt ttaggtgagc gactacgact ggccctgcaa gaactggggc
    12481 cggtttggat caagttcggg caaatgttat caacccgccg cgatcttttt ccaccgcata
    12541 ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc aagctggcga
    12601 agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt gacgattttg
    12661 aaatcaagcc gctggcttct gcttctatcg cccaggttca taccgcgcga ttgaaatcga
    12721 atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtc attaaagcgg
    12781 atctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc
    12841 gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat gaactgaatt
    12901 tgctgcggga atctgccaac gccattcagc ttcggcgcaa ttttgaagac agcccgatgc
    12961 tctacatccc ggaagtttac cctgactatt gtagtgaagg gatgatggtg atggagcgca
    13021 tttacggcat tccggtgtct gatgttgcgg cgctggagaa aaacggcact aacatgaaat
    13081 tgctggcgga acgcggcgtg caggtgttct tcactcaggt ctttcgcgac agctttttcc
    13141 atgccgatat gcaccctggc aacatcttcg taagctatga acacccggaa aacccgaaat
    13201 atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa cgctatctgg
    13261 cagaaaactt tatcgccttc tttaatcgcg actatcgcaa agtggcagag ctacacgtcg
    13321 attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgcc attcgtacgg
    13381 tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat gtactgttaa
    13441 atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg gtgttactcc
    13501 agaaaaccct gctctatgtc gaaggggtag gacgccagct ttatccgcag ctcgatttat
    13561 ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt attcctgccc
    13621 tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg
    13681 aactggttta cgacagtttg cggcagggca agtatttaca gcaccgtgtt gataagattg
    13741 cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt ctcggaattg
    13801 gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgag tgggggctga
    13861 tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac
    13921 gctgattttt tcatcgctca aggcgggccg tgtaacgtat aatgcggctt tgtttaatca
    13981 tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta ttgattattg
    14041 ctgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt tccgatcttg
    14101 gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa
    14161 ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg gatacgaatc
    14221 aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg taatccgtgt
    14281 ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc gttctggggc
    14341 cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac
    14401 tggcgacaac ggtgcagaac gaactgaccc aggagttaaa actccaggag tttcaggaca
    14461 gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac gcccgaactg aaagcgtcga
    14521 tggatgaact acgccaggcc gcggagtcga tgaagcgttc ctacgttgca aacgatcctg
    14581 aaaaggcgag cgatgaagcg cacaccatcc ataacccggt ggtgaaagat aatgaagctg
    14641 cgcatgaggg cgtaacgcct gccgctgcac aaacgcaggc cagttcgccg gaacagaagc
    14701 cagaaaccac gccagagccg gtggtaaaac ctgctgcgga cgcggaaccg aaaaccgctg
    14761 caccttcccc ttcgtcgagt gataaaccgt aaacatgtct gtagaagata ctcaaccgct
    14821 tatcacgcat ctgattgagc tgcgtaagcg tctgctgaac tgcattatcg cggtgatcgt
    14881 gatattcctg tgtctggtct atttcgccaa tgacatctat cacctggtat ccgcgccatt
    14941 gatcaagcag ttgccgcaag gttcaacgat gatcgccacc gacgtggcct cgccgttctt
    15001 tacgccgatc aagctgacct ttatggtgtc gctgattctg tcagcgccgg tgattctcta
    15061 tcaggtgtgg gcatttatcg ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc
    15121 gctgctggtt tccagctctc tgctgtttta tatcggcatg gcattcgcct actttgtggt
    15181 ctttccgctg gcatttggct tccttgccaa taccgcgccg gaaggggtgc aggtatccac
    15241 cgacatcgcc agctatttaa gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt
    15301 tgaagtgccg gtggcaattg tgctgctgtg ctggatgggg attacctcgc cagaagactt
    15361 acgcaaaaaa cgcccgtatg tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc
    15421 gccggatgtc ttctcgcaaa cgctattggc gatcccgatg tactgtctgt ttgaaatcgg
    15481 tgtcttcttc tcacgctttt acgttggtaa agggcgaaat cgggaagagg aaaacgacgc
    15541 tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg cccgtcaggg cggttgtcat
    15601 atggagtaca ggatgtttga tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac
    15661 cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta atgggctact catcaccggc
    15721 actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg
    15781 tcaacggcgg gcgtacatcc tcacgacagc agccagtggc aagctgcgac tgaagaagcg
    15841 attattgagc tggctgcgca gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt
    15901 aaccgcaact tttcgacgcc ggaagagcag gaacgcgctt ttgttgctca gctacgcatt
    15961 gccgcagaat taaacatgcc ggtatttatg cactgtcgcg atgcccacga gcggtttatg
    16021 acattgctgg agccgtggct ggataaactg cctggtgcgg ttcttcattg ctttaccggc
    16081 acacgcgaag agatgcaggc gtgcgtggcg cgtggaattt atatcggcat taccggttgg
    16141 gtttgcgatg aacgacgcgg gctggagctg cgggaattgt tgccgttgat tccggcggag
    16201 aaattgctga tcgaaacaga tgcgccgtat ctgctcccgc gcgatctcac gccaaagcca
    16261 tcatcccggc gcaacgagcc agcccatctg ccccatattt tgcaacgtat tgcgcactgg
    16321 cgtggagaag atgccgcatg gctggctgcc accacggatg ccaatgtcaa aacactgttt
    16381 gggattgcgt tttagagttt gcggaactcg gtgttcttca cactgtgctt aatctcttta
    16441 ttaataagat taagcaatag catggagcga gcctcaccat cgggttcggt gaaaatggcc
    16501 tgaaagcctt cgaacgcgcc ttcggtaata atcaccttat cacccggata agggggggcc
    16561 ggatcgacaa tgtctttcgg tttatatacc gatagctgat gaatcaccgc cgatgggact
    16621 atcgctggcg acgcgccaaa gcgcacaaag tggctgacgc cacgcgtagc gctgatggtc
    16681 gtggtatgaa tcacttctgg atcaaattcc acaaacaggt agttagggaa taacggttca
    16741 ctgactgcgg tacgtttacc ccgtacgatt ttttccaggg tgatcatcgg agcgaggcaa
    16801 ttaaccgcct gtctctcaag gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac
    16861 agtaaatacc aggattgcat aatgactctt atccgtttaa tcggggcgca aggatagcaa
    16921 aagctttacg ctaagttaat tatattcccc ggtttgcgtt ataccgtcag agttcacgct
    16981 aatttaacaa atttacagca ttgcaaagat gaacgccgta taatgggcgc agattaagag
    17041 gctacaatgg acgccatgaa atataacgat ttacgcgact tcctgacgct gcttgaacag
    17101 cagggtgagc taaaacgtat cacgctcccg gtggatccgc atctggaaat cactgaaatt
    17161 gctgaccgca ctctgcgtgc tggtgggcct gcgctgttgt tcgaaaaccc taaaggctac
    17221 tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc gcgtggcgat gggcatgggg
    17281 caggaagatg tttcggcgct gcgtgaagtt ggtaaattat tggcgtttct gaaagagccg
    17341 gagccgccaa aaggtttccg cgacctgttt gataaactgc cgcagtttaa gcaggtgtta
    17401 aacatgccga caaagcgact gcgtggtgcg ccctgccaac aaaaaatcgt ctctggcgat
    17461 gacgtcgatc tcaatcgcat tcccattatg acctgctggc cggaagatgc cgcgccgctg
    17521 attacctggg ggctgaccgt aacgcgcggc ccgcataaag agcggcagaa tctgggcatt
    17581 tatcgccagc agctgattgg taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc
    17641 ggcgcgctgg attatcagga gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct
    17701 gtggcgctgg gtgccgatcc tgccacgatt ctcggtgcag tcacccccgt tccggatacg
    17761 ctttcagagt atgcgtttgc cggattgcta cgcggcacca aaaccgaagt agtaaagtgt
    17821 atttccaatg acctcgaagt gccagccagt gccgaaatcg tcctggaagg gtatatcgat
    17881 cctggtgaga tggcgccgga agggccgtat ggtgaccaca caggttacta taatgaagtc
    17941 gatagtttcc cggtggttac cgtgacgcat attacccagc gtgaagatgc gatttaccat
    18001 tccacctata ccgggcgtcc gccagatgag cccgcggtac tgggcgtggc gttgaacgaa
    18061 gtgtttgtac cgattctgca aaaacagttc ccggaaattg tcgattttta cctgccgccg
    18121 gaaggctgtt cttatcgtct ggcggtagtg acgatcaaaa aacagtacgc cggacacgcg
    18181 aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt ttatgtacac taaatttgtg
    18241 atcgtttgcg atgatgacgt caacgcacgc gactggaacg atgtgatttg ggcgattacc
    18301 acccgtatgg acccggcgcg ggatactgtt ctggtagaaa atacgcctat tgattatctg
    18361 gattttgcct cgcctgtctc tgggctgggt tcaaaaatgg ggctggatgc cacgaataaa
    18421 tggccggggg aaacccagcg tgaatgggga cgtcccatca aaaaagatcc agatgttgtc
    18481 acgtatattg acgccatctg ggatgaactg gctattttta acaacggtaa aagcgcctga
    18541 tgcgcgtttg ttttgcccta tttatcgatc cgacagagaa agcgcatgac aaccttaagc
    18601 tgtaaagtga cctcggtaga agctatcacg gataccgtat atcgtgtccg catcgtgcca
    18661 gacgcggcct tttcttttcg tgctggtcag tatttgatgg tagtgatgga tgagcgcgac
    18721 aaacgtccgt tctcaatggc ctcgacgccg gatgaaaaag ggtttatcga gctgcatatt
    18781 ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg accgcatcct caaagatcat
    18841 caaatcgtgg tcgacattcc ccacggagaa gcgtggctgc gcgatgatga agagcgtccg
    18901 atgattttga ttgcgggcgg caccgggttc tcttatgccc gctcgatttt gctgacagcg
    18961 ttggcgcgta acccaaaccg tgatatcacc atttactggg gcgggcgtga agagcagcat
    19021 ctgtatgatc tctgcgagct tgaggcgctt tcgttaaagc accctggtct gcaagtggtg
    19081 ccggtggttg aacaaccgga agcaggctgg cgtgggcgta ctggtaccgt gttaacggcg
    19141 gtattgcagg atcatggtac gctggcagag catgatatct atattgccgg acgttttgag
    19201 atggcgaaaa ttgcccgtga cctgttttgc agtgagcgta atgcgcggga agatcgcctg
    19261 tttggcgatg cgtttgcatt tatctgagat ataaaaaaac ccgcccctga caggcgggaa
    19321 gaacggcaac taaaacttta ttcagtggca tttagatcta tgtcgcatcc ggcaagtggt
    19381 taaacccgct caaacaccgt cgcaataccc tgacccagac cgatacacat cgtcgccaga
    19441 ccaaactgaa cgtctttgcg ttccatcaga ttcagcagcg tggtgctgat acgcgcaccg
    19501 gaacaaccca gcggatgacc cagcgcgatc gcaccaccat tgaggttgat cttctcgtca
    19561 atctgctcca ttagcccgag atctttaatg cacggcagga tctgcgcggc aaaggcttcg
    19621 ttcatctcaa acacgccgat atcgctggca gaaagccccg cttttttcag cgccagcttc
    19681 gaggccggaa ccgggccgta acccataatc gatgggtcac aaccaacgac cgccatcgaa
    19741 cgcacacgag cgcgcggctt aagacctaat tcatgggcgc ggctttcact catcaccagc
    19801 atggcagctg cgccatcgga aagtgcagaa gatgtgcccg ccgttaccgt accgtttact
    19861 ggatcaaacg ccggacgcag cgtggcgagg gcttccacgg tggtttccgg gcgaatcact
    19921 tcgtcgtaat taaactgctt caggacgccg tcggcatcgt gaccaccagt tgggatgatt
    19981 tcatttttaa atgcggccga ctgcgtggcg gcccaggcgc gggcgtgtga ccgcgcggca
    20041 aaggcatcct gcatttcacg gctgataccg tgtatacgcg ccagcatttc tgccgttaag
    20101 cccatcatgc ccgccgcttt ggcgacattg cggctcaggc cggggtgaaa atcgacgccg
    20161 tgactcatcg gcacatggcc catatgctcc acgccgccaa ccagacatgc ctgcgcatcg
    20221 ccagtcatga tcattcgtgc tgcgtcatgc agtgcctgca tggatgaacc acacaagcga
    20281 ttgacggtaa ccgccgggac agagtgcggc acttctgcca gcagcgccgc gttacgggcg
    20341 atgttaaaac cctgctccag cgtctgttgc acacaacccc agtaaatatc gtcgagggcc
    20401 gccgcttcca gcgccgggtt acgcgccagc aggctacgca ttaaatgagc ggagagatct
    20461 tccgcacgca cgttacgaaa agcaccgccc ttcgaacggc ccatcggggt gcgaattgca
    20521 tcgacaatga caacctgttc cattgtgatt ccttaagccg ttttcaggtc gccaaccgga
    20581 cgggctggct caaccggagg atagtacggt tcgttatgac gcgctttatt acgcagacct
    20641 tccggcactt catacagcgg gccgaggtgc tgatattgct gtgccatatc gaggtatttt
    20701 gcgctaccga gggtgtccag ccagcggaac gcgccgccgt ggaacggagg gaagcccagg
    20761 ccgtagacca gcgccatatc cgcttccgcc ggagtggcga taatgccctc ttccagacag
    20821 cgcaccactt cgttgaccat cgggatcatc atgcgggcga taatctcttc ttcgctgaaa
    20881 tcgcgcttcg gttggctcac ttctgccagc aggtcttcaa cggcggcgtc ttcttctttc
    20941 ttcggcttac ctttgctgtc ttctttataa cgccagaaac cgaggccgtt cttctgacca
    21001 aagcggttgg catcaaacag cgcgtcgatg gcatcgcggt aatctttctg catccgctgc
    21061 gggaagcctg ctgccatgac agcctgagcg tgatgcgcgg tatcaatgcc cacaacgtcc
    21121 agcagatatg ccgggcccat cggccagcca aactgttttt ccatcacttt gtcgatcttg
    21181 cggaaatccg cgccgtcgcg cagcagctgg ctgaaaccgg cgaaatacgg gaacagcacg
    21241 cggttaacaa agaagccggg gcagtcgtta accacaatcg gcgtcttgcc catcttgctc
    21301 gcccaggcga caactttcgc gatggtttcg tcggagcttt tctcgccgcg aataatttct
    21361 accaacggca ttcggtggac cgggttaaag aagtgcatcc cgcagaagtt ttccgggcgt
    21421 tccagcgcgt tggccagttc gctgatagga atggttgaag tgttagacgc cagcacggta
    21481 tcctggcgta ctttttgttc ggtttctgcc agtacggctt ttttcacttt cgggttttca
    21541 acaaccgctt ctaccacaat atccacgcgg tcaaatccgg cgtagtcgag cgttgggtgg
    21601 atcgtggaga tcacgccagc cagtttcaga ccatcgatct tgccgcgctc aagctgcttg
    21661 ttcagcagtt tcgcggcttc ggtcatgccg agggttaacg acttgtcgtt gatatctttc
    21721 atgacaaccg gcacgccttt ccacgcggac tggtaagcga taccgccacc catgatgcct
    21781 gcgcccagca cagcagcctg tttcggggtt tcaacgtctt tggtgagttt cttcgctttg
    21841 ccttttacat attgatcgtt aaggaaaatg ccgaccagtg cgcgagcttc gttggtatgc
    21901 gccagcggga caaaactttt gttttccaga tttaaggctt cttcacgacc aaaacgggcc
    21961 gcagcttcaa tggtttttac tgcggtgatg ggggccggat aatgtttccc cgccgtttgc
    22021 gcgaccattc ctttagcgat ggtgaagctc atggtggctt caatcttgct cagttttagc
    22081 ggttccagct tcggctgacg tttcgctttc cagtcgaggt cgccgttaat ggcctggcgt
    22141 aaaatcgcca tcgccccttc aatcagtttt tctgctttga ctacgccatc caccagaccg
    22201 attttcagcg cctgatccgc gccgacatct ttaccggcgg cagtgatttc cagtgcactg
    22261 tcagcgccca gcatacgtgg catacgtaca gaaccgccaa agcccggcat gatgcccagt
    22321 ttggtttccg gcagaccgat gcgcagatcc ggcgtcgcca gacgataatc ggtcgccagc
    22381 acgcattcgc agccaccacc cagcgcatag ccgttgacgg cagcaatggt cggcaccggc
    22441 agatcttcca ggcgattaaa cacgctattg gcaaagtgca gccactgact taactgttct
    22501 tcaggaacga ggaacaggga caaaaattcg gtgatatcag caccgacgat aaaggctgct
    22561 ttgttcgaac gcagcagcag cccttttaga tctgattgct gttccagcac gccgatggcc
    22621 tcgccgaggc tggcgacggt cgcagtgtcg agtttattaa ctgaacctgg ggcatcaaat
    22681 accagttcgg caatgccatc ttccagccag tcaaggtaca gggtgtcgcc tttgtaaagc
    22741 atgtcagtct cctgaatccg caaggtgatc tggtcgtacc agatgcatcg gagtgtgtgt
    22801 tttgtgttaa aaatatgcaa ataaaagatt aaagaaatgc cgatctgatc acgctcggca
    22861 gaaatcacgc tctggatgaa cgatgtgcta agatgcggag acttaaggtc aaaaaaacag
    22921 aagggtaaaa aatggaatca ctggcctcgc tctataaaaa tcatatagct accttacaag
    22981 aacggactcg cgatgcgctg gcgcgcttca agctggatgc attacttatt cactccggcg
    23041 agctgttcaa cgtttttctc gacgatcatc cctatccgtt taaagtgaac ccgcaattca
    23101 aagcgtgggt gccggtaact caggtgccaa actgctggtt gctggtggat ggcgtgaaca
    23161 agccgaaact gtggttctat ctgccggttg attactggca caacgtcgaa ccgctgccga
    23221 cctccttctg gactgaagat gtggaagtga tcacgctgcc gaaagccgat ggcattggta
    23281 gcctgctgcc tgctgcgcgc ggcaatatcg gttatatcgg tccggtgccg gaacgtgcgc
    23341 tgcaactggg tattgaggcc agcaacatca acccgaaagg ggtgatcgac tacctgcatt
    23401 attaccgctc cttcaaaacc gagtacgaac tggcctgtat gcgtgaagcg cagaaaatgg
    23461 cggtcaatgg tcatcgtgcg gcagaagaag cgttccgttc tggcatgagc gagttcgata
    23521 tcaatattgc ctatctgacc gcgaccggtc atcgtgatac cgacgtacct tacagcaaca
    23581 ttgtggcgct taacgaacac gctgcggtgt tgcattacac caaactggac caccaggcac
    23641 cggaagagat gcgcagcttc ctgctggatg ccggggccga atataacggc tatgcggctg
    23701 acctgacccg tacctggtcg gcaaaaagcg acaacgacta cgcacagctg gtgaaagacg
    23761 taaatgatga acaactgggg ctgatcgcca ccatgaaagc aggcgtcagc tatgtggatt
    23821 accacatcca gttccatcag cgcatcgcca aactgctgcg taaacatcaa atcatcaccg
    23881 atatgagtga agaagcgatg gtcgaaaacg atctcaccgg accgtttatg ccgcacggta
    23941 ttggccatcc gctgggcctg caggtgcatg acgtcgccgg ttttatgcag gatgatagcg
    24001 gtacacacct cgcggcaccg gcaaaatatc cgtacctgcg ctgcacccgt attctccagc
    24061 cgggcatggt gttaaccatc gaaccgggta tctacttcat tgaatcgctt ctggcaccgt
    24121 ggcgtgaagg gcagttcagc aagcacttca actggcagaa aattgaagca ctgaaaccgt
    24181 tcggcggcat tcgtatcgaa gacaacgtgg tgatccacga aaacaacgtg gaaaacatga
    24241 cccgggatct gaaactggcg tgatggaaag ctggttaatt cctgcggcac cggtcacggt
    24301 cgttgaagag atcaaaaaga gccgcttcat aacgatgttg gcgcataccg atggcgttga
    24361 ggcggcgaaa gcgtttgttg aatcggtgcg ggcagaacac cccgatgccc gccaccattg
    24421 cgtggcgtgg gtggcgggtg cgccggatga ttctcaacag ctgggtttct ctgacgacgg
    24481 ggagccggcg ggaacggcag gtaaaccgat gctcgcccag ctaatgggca gcggcgtcgg
    24541 ggaaattacc gctgtggtag tgcgctacta cggcggcata ttgcttggca ccggtgggtt
    24601 agtgaaagcg tatggcggcg gcgtgaatca ggcgctgcgc cagctaacga cccaacgcaa
    24661 gacgccatta accgaatata ctttgcaatg tgaatatagt cagttaaccg gcattgaagc
    24721 gttgctgggg cagtgtgacg gcaaaattat caacagtgat tatcaggcat tcgttctgct
    24781 gcgggtggcg cttccggcgg cgaaagtggc tgaattttcc gcaaagctgg cggattttag
    24841 ccgtggttca ttgcaattgt tagcgattga agaataatcc ccacttcgtt ttgcagaact
    24901 aaggaagcgg cagagatgca ttttcgcgcc attacccgaa tcgttggact actggtcatc
    24961 ttattttcag ggaccatgat tatccctggg ctggtagcac tcatctaccg ggatggagcg
    25021 ggccgcgctt ttacccagac cttttttgtc gccctcgcca ttggctctat gctgtggtgg
    25081 ccgaaccgca aagagaaggg cgaactgaaa tcccgtgagg ggtttctgat agtggtgctg
    25141 ttttggaccg tgctgggtag tgtcggtgcg ctccctttta tcttctcgga aagcccgaac
    25201 ctcacgatta ccgatgcgtt ttttgaatct ttctctggcc tgaccaccac cggggccact
    25261 acgctggtgg ggctggattc gctccctcat gccatcctct tttatcgcca gatgctgcaa
    25321 tggtttggcg ggatggggat catcgtgtta gcggttgcga tactgcctat cctcggcgtg
    25381 ggtgggatgc agctctatcg cgcagaaatg cccggcccgc tgaaagataa caaaatgcgc
    25441 ccgcgaattg cggaaacggc gaaaactctg tggttgattt atgtcttgct gaccgtcgcc
    25501 tgtgcgctgg cgttgtggtt tgccggaatg gatgcctttg atgccatcgg ccatagcttt
    25561 gcgactatcg ctattggcgg cttctcgaca catgatgcca gtatcggtta tttcgacagt
    25621 ccggctatta acactatcat tgctatcttc ctgctgatct ccggctgtaa ctacggtctg
    25681 cacttttcac tgttaagtgg gcgtagtctg aaggtttatt ggcgcgatcc ggaatttcgc
    25741 atgtttatcg gcgtacagtt tacgctggtg gttatttgta cactcgtact gtggtttcat
    25801 aatgtctaca gttcggcgct gatgacaatt aaccaggcgt ttttccaggt ggtatcgatg
    25861 gcgacaaccg ccgggtttac gactgacagc attgcccgct ggccgctctt tttgccggta
    25921 ctgcttttat gttcagcttt tatcggcggt tgcgccgggt caacgggcgg tggcctgaaa
    25981 gtgatccgca tcctgctgct gtttaagcag gggaaccgtg agctgaaacg actggtgcat
    26041 ccgaacgccg tctatagcat taagctgggg aatcgcgcac tgccggaacg tatcctcgaa
    26101 gccgtttggg gatttttctc cgcctatgca ttggtgttta ttgtcagtat gctggcgatt
    26161 atcgccacgg gcgtggatga tttttctgcc tttgcctcag ttgttgcgac attgaataac
    26221 ctggggccag ggcttggcgt ggttgctgat aactttacca gtatgaaccc ggtggctaaa
    26281 tggatcctga ttgccaacat gctgtttggt cgtctcgagg tctttacatt gctggtgctc
    26341 tttaccccga ctttctggcg tgaatgatgg agtaatacgt gaaaacatta attcttttct
    26401 caacaaggga cggacaaacg cgcgagattg cctcctacct ggcttcggaa ctgaaagaac
    26461 tggggatcca gacggatgtc gccaatgtgc accgcattga agaaccacaa tgggaaaact
    26521 atgaccgtgt ggtcattggt gcttctattc gctatggtca ctaccactct gcgttccagg
    26581 aatttgtcaa aaaacatgcg acgcgactga attcgatgcc gagcgccttt tattccgtaa
    26641 acctggtggc gcgcaaaccg gagaagcgta ctccacagac caacagctac gcgcggaagt
    26701 ttctgatgaa ctcgcaatgg cgtcccgatc gctgcgcggt cattgccggg gcgctgcgtt
    26761 acccacgtta tcgctggtac gaccgtttta tgctcaagct gattatgaag atgtcaggcg
    26821 gtgaaacgga tacgcgcaaa gaagttgtct ataccgattg ggagcaggtg gcgaatttcg
    26881 cccgagaaat cgcccattta accgacaaac cgacgctgaa ataagcataa agaa
//
LOCUS       DADLZM010000044        26411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLZM010000044 DADLZM010000000
VERSION     DADLZM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.044"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            118..1089
                     /locus_tag="JNY73_001812"
     CDS             118..1089
                     /locus_tag="JNY73_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY8047844.1"
                     /translation="MRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVR
                     QGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVR
                     RASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKI
                     AVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLN
                     AIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKM
                     GNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1112..2623
                     /locus_tag="JNY73_001813"
     CDS             1112..2623
                     /locus_tag="JNY73_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8047845.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2648..3646
                     /locus_tag="JNY73_001814"
     CDS             2648..3646
                     /locus_tag="JNY73_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8047846.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTGLSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3679..4773
                     /locus_tag="JNY73_001815"
     CDS             3679..4773
                     /locus_tag="JNY73_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY8047847.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4785..5657
                     /locus_tag="JNY73_001816"
     CDS             4785..5657
                     /locus_tag="JNY73_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY8047848.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5705..6127)
                     /locus_tag="JNY73_001817"
     CDS             complement(5705..6127)
                     /locus_tag="JNY73_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY8047849.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6224..7430)
                     /locus_tag="JNY73_001818"
                     /pseudo
     CDS             complement(6224..7430)
                     /locus_tag="JNY73_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="JNY73_001819"
     CDS             complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="JNY73_001819"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY8047850.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8249..8569)
                     /locus_tag="JNY73_001820"
     CDS             complement(8249..8569)
                     /locus_tag="JNY73_001820"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY8047851.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8569..9087)
                     /locus_tag="JNY73_001821"
     CDS             complement(8569..9087)
                     /locus_tag="JNY73_001821"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY8047852.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9084..10445)
                     /locus_tag="JNY73_001822"
     CDS             complement(9084..10445)
                     /locus_tag="JNY73_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY8047853.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10581..11471
                     /gene="hcaR"
                     /locus_tag="JNY73_001823"
     CDS             10581..11471
                     /gene="hcaR"
                     /locus_tag="JNY73_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY8047854.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11631..12770
                     /locus_tag="JNY73_001824"
     CDS             11631..12770
                     /locus_tag="JNY73_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY8047855.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="JNY73_001825"
     CDS             complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="JNY73_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY8047856.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKHFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAVLAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(14233..15087)
                     /locus_tag="JNY73_001826"
     CDS             complement(14233..15087)
                     /locus_tag="JNY73_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8047857.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTSPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="JNY73_001827"
     CDS             complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="JNY73_001827"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY8047858.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16154..16894
                     /gene="trmJ"
                     /locus_tag="JNY73_001828"
     CDS             16154..16894
                     /gene="trmJ"
                     /locus_tag="JNY73_001828"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY8047859.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17164..17652
                     /gene="iscR"
                     /locus_tag="JNY73_001829"
     CDS             17164..17652
                     /gene="iscR"
                     /locus_tag="JNY73_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY8047860.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17764..18978
                     /gene="iscS"
                     /locus_tag="JNY73_001830"
     CDS             17764..18978
                     /gene="iscS"
                     /locus_tag="JNY73_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY8047861.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19006..19392
                     /gene="iscU"
                     /locus_tag="JNY73_001831"
     CDS             19006..19392
                     /gene="iscU"
                     /locus_tag="JNY73_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY8047862.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19409..19732
                     /gene="iscA"
                     /locus_tag="JNY73_001832"
     CDS             19409..19732
                     /gene="iscA"
                     /locus_tag="JNY73_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY8047863.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19828..20343
                     /gene="hscB"
                     /locus_tag="JNY73_001833"
     CDS             19828..20343
                     /gene="hscB"
                     /locus_tag="JNY73_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY8047864.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20360..22210
                     /gene="hscA"
                     /locus_tag="JNY73_001834"
     CDS             20360..22210
                     /gene="hscA"
                     /locus_tag="JNY73_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY8047865.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22212..22547
                     /gene="fdx"
                     /locus_tag="JNY73_001835"
     CDS             22212..22547
                     /gene="fdx"
                     /locus_tag="JNY73_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY8047866.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22559..22759
                     /gene="iscX"
                     /locus_tag="JNY73_001836"
     CDS             22559..22759
                     /gene="iscX"
                     /locus_tag="JNY73_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY8047867.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22818..24101
                     /gene="pepB"
                     /locus_tag="JNY73_001837"
     CDS             22818..24101
                     /gene="pepB"
                     /locus_tag="JNY73_001837"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY8047868.1"
                     /translation="MTEAMKITISTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24243..25019
                     /gene="sseB"
                     /locus_tag="JNY73_001838"
     CDS             24243..25019
                     /gene="sseB"
                     /locus_tag="JNY73_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY8047869.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="JNY73_001839"
                     /pseudo
     CDS             complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="JNY73_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6256 a         6586 c         7312 g         6257 t
ORIGIN      
        1 cgttcactaa accctattaa ctgtaatatt tgagcggcac gcgctgccgc tcatcacaaa
       61 ataattagaa taatccgttg ttaataaaaa ccctacagga ggcactatgc ctaaaaaatg
      121 agaacaaccc gtaatctatt attaatggct acgctattag gtagcgcact atttgccagg
      181 gctgcggata aagaaatgac cattggtgca atataccttg atacccaggg atattacgct
      241 ggagtgcgtc agggcgttca ggatgcggca aaagattctt cagtacaggt acagttaatt
      301 gaaactaacg cccaggggga tatttcgaaa gaaagtacct ttgttgatac cctcgtggcg
      361 cgtaatgtcg atgccattat tttatcggca gtgtctgaaa atggcagtag ccgtaccgtt
      421 cgtcgcgcca gtgaagcggg tattccggtg atttgctaca acacctgtat taatcaaaag
      481 ggtgtcgata aatatgtctc ggcgtatctg gtcggcgatc cactggaatt tggtaaaaaa
      541 ctgggtaacg ctgccgccga ttattttatt gccaataaaa ttgaccagcc gaaaattgcc
      601 gtcatcaatt gcgaagcctt tgaagtttgt gtgcagcgac gtaaaggatt tgaagaagta
      661 ttaaaatccc gcgttcccgg cgcgcaaatt gtcgctaacc aggaagggac tgttttagat
      721 aaagcgattt ccgttggtga aaaactgatt atctccacgc cggatctcaa cgccattatg
      781 ggggagtcgg gcggtgcgac actcggcgcg gtaaaagcgg tacgtaatca aaatcaggcc
      841 ggaaaaattg ctgttttcgg ttcggatatg acaaccgaaa ttgctcagga gctggaaaac
      901 aatcaggtgc tgaaagcggt ggtggatatt tccggtaaga aaatgggcaa tgctgttttc
      961 gcgcaaacat tgaaggttat caataaacaa gccgacggtg aaaaagtgat tcaggtgcca
     1021 atcgatctct ataccaaaac ggaagatggt aaacagtggc tggcaacgca cgttgatggt
     1081 ctgccctaat cgctgattcg cagaggtccc tatgttcacg gcaacagagg cagtcccggt
     1141 agcaaaagtg gtggcaggaa ataagcgtta ccccggcgtc gttgcgctgg ataacgttaa
     1201 cttcacgctc aataaaggcg aagttcgtgc gctgttaggt aaaaacggcg cgggcaaatc
     1261 gactctcatt cgaatgctta ccggcagcga acgcccggat agcggtgata tctggattgg
     1321 cgagacgcga ctggaaggtg acgaagctac gttgactcgc cgtgccgctg aactgggggt
     1381 tcgtgcggtt tatcaggaat taagtctggt ggaagggctg acggtggcag aaaacctctg
     1441 ccttggtcaa tggcctcgcc gcaacggcat gattgattac ctgcaaatgg cgcaggatgc
     1501 ccaacgttgc ttacaggcgc tgggcgttga cgttagtcct gaacaacttg tttcaacgct
     1561 aagcccggcg caaaagcaac tggtggaaat tgcacgggtg atgaagggcg agccgcgcgt
     1621 ggtcattctt gatgaaccca ccagctcgct tgccagtgca gaagttgaac tggtgatcag
     1681 tgcggtgaaa aagatgtcgg cactgggcgt ggcggtgatt tatgtcagcc accggatgga
     1741 agaaatccgc cgcattgcct cctgtgccac cgttatgcgc gatggtcagg tggcgggcga
     1801 tgtgatgctc gaaaacacct ccacgcatca tattgtgtcg ctaatgctag ggcgtgatca
     1861 cgttgatatt gcgccggttg cccctcagga aattgtggat caggccgtac tggaagtccg
     1921 tgcgttacgc cataagccca agctggagga tatcagcttt acgctacgtc gtggcgaagt
     1981 gcttggcatt gctggcctgc tgggggcagg gcgcagtgaa ttgctgaagg cgattgttgg
     2041 gctggagacg tatgaacagg gcgaaattgt tatcaacggc gagaaaatca tgcgccccga
     2101 ttacggcgac atgctgaaac gcggcattgg ctatacgcca gaaaaccgca aagaagcggg
     2161 gatcattccc tggctgggcg ttgacgaaaa tacagtgctg accaatcggc aaaaaatcag
     2221 caccaacggt gtgctgcaat ggtccaccat ccgccgcctg accgaagagg tgatgcagcg
     2281 gatgacggtc aaggctgcca gtagcgaaac acccatcggc acgctttctg gtggcaatca
     2341 gcaaaaagtg gtgatcggtc gttgggtcta tgccgccagc cagattttgt tgctcgacga
     2401 gccaacgcgc ggcgtcgata tcgaagccaa acagcagatt taccgtattg tccgtgagct
     2461 ggctgccgaa ggaaaaagcg tggtgtttat ctccagtgaa gtggaggaat tgccgctggt
     2521 gtgtgaccgc atcctgttat tacagcacgg cacgttctcg caggagtttc actcgccggt
     2581 caatgtggat gagctgatgt ccgccattct gtctgtgcac tgatgaattt taccgaggaa
     2641 cctgaagatg tctgcttcgt cattaccatt gccgcagggc aagagcgtct cgctcaaaca
     2701 atttgtcagt cgccatatta atgagatcgg tttgctggtg gtgattgcca ttctttatct
     2761 ggtcttctcc ctgaacgcgc ctggctttat ctcattgaat aaccagatga acgtgctgcg
     2821 cgatgccgcc accattggga ttgccgcctg ggcgatgacg ctgattatta tctccggtga
     2881 aattgatgtc agcgttgggc cgatggtggc ttttgtctcg gtgtgcctgg catttttgct
     2941 gcaatttgaa gttccgttgg cgattgcttg tctgctggtg ttgctgttag gcgcgctgat
     3001 gggaacgctc gccggggtgc tgcgcggcgt gtttaacgta ccaagtttcg ttgccacact
     3061 ggggctgtgg agcgctctgc gcggaatggg gctgtttatg accaacgcac agccagtgcc
     3121 gattgacgaa aacgaggtgc tggactggct gggcggacaa tttctcggtg tgccagtatc
     3181 cgcgctgatc atgatggtgt tgtttgcgct gtttgtgttc attagccgaa aaaccgcctt
     3241 cgggcgctcg gtttttgccg ttggcggtaa tgccacggcg gcgcagttgt gcggtattaa
     3301 cgttcgtcga gtgcgcattc ttatctttac cctttcggga ttattagcgg cggtgaccgg
     3361 cattttgttg gcggcacgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt
     3421 tgacgtcatc gccgcggtcg tggtcggcgg tacgggactt tccggcggtc gcggttccct
     3481 gttcggtaca ttgcttggtg tgctggtgat tacgctaatc ggtaacggtc tggtgctgct
     3541 cggtattaac tcctttttcc agcaggtggt acgcggcgtc atcatcgtgg tggcggtgct
     3601 ggcgaatatc ttgctgaccc agcgaagcag taaagcgaaa cgctaaacct taacgcccct
     3661 ggcgcgggtg cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg
     3721 atgctggcag cttatttacc aggaaattca accgtcgatc tgcgggaagt tgcggtgccg
     3781 acgccgggga ttaaccaggt actgatcaaa atgaaatcct ccgggatttg cggaagcgat
     3841 gtccactata tctatcatca acaccgtgcc acggcggcgg cacccgataa accgttatac
     3901 cagggattta tcaacggtca tgaaccgtgc gggcagattg tggcgatggg gcaaggctgc
     3961 cgccatttta aagagggcga ccgcgtgctg gtgtatcaca tttctggctg tggtttttgc
     4021 ccgaactgcc gccgcggctt tcctatctct tgtactggca aaggaaaagc ggcttacggc
     4081 tggcagcgtg acggcggtca tgccgaatat ctgctggcgg aagaaaaaga tctgatcctc
     4141 ctgccggatg cgctgagcta cgaagatggt gcgtttatca gttgcggcgt tggcacggcc
     4201 tatgaaggga ttttgcgcgg cgaagtttcc ggcagcgata acgtgctggt ggtcggtctg
     4261 ggggcggtcg gcatgatggc gatgatgctg gcgaaaggtc gcggtgcaaa acggatcatc
     4321 ggcgttgata tgctgccgga acgtctggcg atggcaaaac agttaggggg gatggatcac
     4381 ggctatttag caaccaccga aggtctgccg cagataattg ctgaacttac ccacggtggc
     4441 gcggatgttg cgctcgattg ttccggtaat gccgcaggtc gcttgctggc actgcaatcc
     4501 actgctgact gggggcgggt ggtttacatt ggtgaaaccg gaaaagtgga attcgaggtc
     4561 agcgctgatc tgatgcacca tcaacggcgg attatcggct cctgggtgac cagtctgttc
     4621 catatggaaa aatgcgccca cgatttgact gactggaagc tgtggccgcg taacgccatt
     4681 acccatcgct tctcgctaga acaggcaggt gatgcctatg tgctgatggc gagcggcaaa
     4741 tgcgggaaag ttgtgattaa cttcccggat taaggagtta gcggatgacc atctatacct
     4801 tatcccacgg gtcgctaaag ctggacgttt ctgaccaggg cggcgtaatc gaaggcttct
     4861 ggcgcgatac gacgccgctc ttacgtcctg gtaaaaaaag cggtgtggca accgatgcct
     4921 catgttttcc gctggtgcca tttgcaaacc gggtgagcgg caaccgtttt gtctggcagg
     4981 ggcgtgagta tcaactgcaa ccgaatgttg agtgggatgc gcactatcta cacggcgatg
     5041 gctggctggg cgagtggcaa tgtgtctcgc acagcgatga tagtttgtgt ctggtgtatg
     5101 aacatcgcag cggtgtctat cactatcggg taagtcaggc gtttcattta acggcggata
     5161 cgctgacggt gacgctctct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg
     5221 gctggcatcc ttattttccg ttgtcaccac aaacgcggat tcaggcgcag gcgagcggtt
     5281 actggctgga acgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac
     5341 tggattttaa ccagctcgcg ccgttgccgc gtcagtgggt gaacaatggt tttgccggat
     5401 ggaatggtca ggcccgcatc gagcagccgc aggaaggtta tgccatcatc atggaaacga
     5461 cgccacctgc accgtgttat ttcatctttg tttccgaccc tgcgtttgat aaaggatatg
     5521 cgtttgattt cttctgtctg gaaccgatga gccatgcgcc ggatgatcat catcgcccgg
     5581 aagggggtga tctcattgcg ctagcaccag gggaatcaac aacctcagag atgtcgttgc
     5641 gggtagagtg gctgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc
     5701 tttcctaacg ccgatcgaga gaaattgccc ccggtccggt aattgccaat agcaagaacg
     5761 cgccagcgat actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg
     5821 tcatatccca gtaatggtga ccaatcaccg ccgtacccag cgtgtagaaa ataaacagca
     5881 ccgccagcgg acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa
     5941 ctaccgcaat aatcgctgcc agcatcggca ttggcgcgcc caacgaggcc atatattgga
     6001 ccgtaccgtc aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg
     6061 cgatacgggc aattaataac aaaacggggc gggcagctcc aaaatcaaaa taacgtagtg
     6121 tgttcataac caatcccttt gcaagataat gtgatttaaa cgtaatacac actcttgcac
     6181 ctcgccacgc cccaggcatt gagatattcg ctaattatcc tggttacagt gatttaagcg
     6241 cgatgttctc atctatcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc
     6301 gaattgggcg aatctcacgc ccctgattca gcgtgaccgc accgataagc acgccgtttt
     6361 gcagattaaa ccaaatcgcc ttctgagttt ccgggttgcc acgacaaagc cagtcatcgc
     6421 cacgcatatc gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg
     6481 gcggcagtag cggtagccct aacattgcgg cagcggcaat ttgcgcctgg ttattggtgt
     6541 tttcccagct ttcgcagcgg tgtagtgcac cattatcaag acgagtgatt gccacatcgc
     6601 caccggcaaa gatcgcggga tcgcaggtgc ggcaagcctc atcaatgaca atgccattgg
     6661 cagtatcaag gttggcctcg cgagccagtt gctcgttggc gctgatacta ataccgtaaa
     6721 tcaccacatc agcctgaagc gtttccccac tttgcagcgt cagttctact ttttcaccat
     6781 cgaccacatg ttcaatggca ttattgagca gaatgcgcac accagcctgc tgatggcgct
     6841 gtaaaagata gcgttgcacg ggcggtggtg cattacggcc catgacggtt gccgccagtt
     6901 caatcactgt caccttacat ctgcgctgcg ctgcgctgcg tggcgctggc agccagttcc
     6961 agaccaatag ttccggcacc gacaatcacg actgaccgtt cgggctgcag aacttctcgc
     7021 agtctggcgg catcaccggc atggtgtagg gtaaagcagc gttctcccag tgcatcaagc
     7081 aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa gctgatccca gtgccagctt
     7141 tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca gcgttttgat ggttacaccg
     7201 gaatgcagat gaacattgtt ttcctgccac cagttagcgg gtaacacctg ctgtaactgc
     7261 ggggaatctt ccagcaacat ggatttcgag agtggaggtc gttcataagg aagatgtcgc
     7321 tcatcggaaa acagatgcag ctcaccggtg aacccttgct ggcgtagcga ggccgcagcc
     7381 attgccgccg cttgcccgcc accgacaata atgatcgttt tttctttcat cgtgcttcct
     7441 taaagatcca gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg
     7501 ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat cacatacggc
     7561 cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta aaatggcggc aattttctcc
     7621 ggcgtcagag actgcattat cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg
     7681 tcgctggcca taccacacgg gccgacgcca ttcacccgca ctttcggtgc cagttcataa
     7741 gccagttggc gaataagtcc ggttgcggca tgtttactgg tggtgtacag cgggccaccg
     7801 ccgccaggat accaggcggc atttgacagt gtgaaaatca tgctgccttc actggcgatt
     7861 aacgccggag cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac gttaaacagc
     7921 tcgtggaagc cggtttcgag cgtctctgcg ggagtattaa ccagtgaggc attgtgatcc
     7981 cagatgcctg cattgccgat aaaacaatcc agcttgccgg aacgagtcag gatctgatcg
     8041 accgcgcgtt gataatcggc ataacaggtc acgttacctt ccaccgccag aatatgttcg
     8101 ccaaatcgct gacgcagact ggcgactttt gccgccgaca gttccagcgc ggcaacctgc
     8161 gcgccttctt cgataaatcg ctcgaccagc gccagcccta atcccgatcc gccgccggta
     8221 ataaaaatgg actcgttatg cagatcgctc atggctgcgc ctccggtaag tcgatgaaaa
     8281 tgtcaccacc ttcaacgtgt actggataag tggtgagcgg atcggtggcg ggcaggcata
     8341 acgctttccc cgttttcagg caaaaactgg cggcgtgtag cgggcactcc accgtggcgt
     8401 catcttccag atacccttct gacatcgacg cattaccatg gctgcaacga tcgttaatgg
     8461 cataaaactc gccgcccacg ttgaacaagg cgatgacggg cgaggtatct atccggagag
     8521 cctcaccctc cggcacatcc gcaacgggac acgcataaat tcgattcatc agaacagtac
     8581 actcaggtta tgggaagtga tcaccgcttg atccaggacg atatcccgtt ccagcaagcg
     8641 ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc
     8701 atcgcgctct ttttgtgccc gataaagtag ataattcacc ctcacggcaa atacgtttgg
     8761 gatgtcggtt tcgcttacct ggcagttgct gattaagtga cgagtgcgtg acggcggctc
     8821 ttctgcccag gccatgcccg tttccagacg ggcgattcgc cgctccagct ggtctttggt
     8881 gtcattgaaa atccaggtcg tcggcggctg gacgcctttg cgacggtcgc gtgtttgcgc
     8941 gttaaccgtg gtgcgcatgg tgtaacgaat ctcttcgtcg agctgtgcca accagtcacg
     9001 aaatttccag tcatccagca gactggcttc gtgaaagaga aactggctaa tgcggtgatg
     9061 taactccagt gaaacttgcg cactcatttc atcacctcct gctggtaagc ggcggtttta
     9121 tcgagcactt cctgccagct ttcgctactc agaagatcgg cccagcgttg gtacattcca
     9181 cgagcggcag tttctgaaaa gatatagtta gtaatgccag gaatgccgtc gtcgcggcgc
     9241 ttttcctgac caagccccat ttccagacac agtttgctgt tgcgggcgcg gtggccttta
     9301 agcaatttct ggatttcaca ccagttctcc gagtcatcct gctcgagaaa accagcagga
     9361 ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa cttcatcgga ggcggctttg
     9421 tcagtaatac agaacgccca cacttcaact tgatcagggc cgcgcggatg ccagacgcgg
     9481 agtgtggcag tgccgtttag ccatgaaagc gtggggaata tattgttatg acccgccagg
     9541 cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg cataggtttc gcggtaatag
     9601 cttgaaactg cgccatcgac ccagacatta gcatccggtt tttcagtaaa gaagaaaccg
     9661 ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg cggtttccca caccgggcgg
     9721 gcggtttgtc catcaccgag gcgcttatcg ctgccatcat ctttcgcccc taatacctga
     9781 acggcagaag catggctgaa cagagcatga tactggtcac tggcgaactg ctctgccggg
     9841 aatttccagt tacagttgat cacccacttt tgtacgccgc cgacaatttc ggtgccgcct
     9901 tcgcgacgat ccagcatgcc atccagatac caggcaatgt cacccaggta atcatgcagg
     9961 cccggtgcgc tggtatccca gttgccaaaa atcagtcctt tataactctc cacacaagga
    10021 acttcgttta gcccccagtg ggatttacac aacccttgtg ggtaggcgcg aggttccagc
    10081 ggtacatcga tcaactcgcc gttaatgcca taagaccagc cgtgatacgg gcaggtaaag
    10141 gcgcgagtgt tgccacaatc tgcataactc acacgcatgg cccggtggcg gcattggtta
    10201 agaaacgcct tgatgctgcc gtctttctgg cgcaccacga caactgcatc ttctcccatg
    10261 taggtgttaa agaaatcacc gggttttggg atctggcttt cgtgggcgag aaatagccag
    10321 caacgaccga aaatacgctc aagctccagt tggtaaatgt ccgggtcggt ataaatacgc
    10381 ggagtgaccc gaccgttttg ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg
    10441 gtcatatttt ttaatcctga aagataagat gtattgacct tctatttttg tgccataagg
    10501 atgttgctgg ttggttgcta atatgtgaaa tagttaattt ctaccagctg agacgttttt
    10561 tcgataacaa ggggaaggtg atggaactac ggcatttacg ctatttcgtc gcagtggcgc
    10621 aggcactgaa ctttacccgt gcggcggaaa aactgcatac ctcacagcct tcgttaagta
    10681 gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt actggtgagg gataagcgca
    10741 aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga cgcgctggca atcctcgaac
    10801 aagcggaaaa tgccaaatta cgggcgcgga aaattgttca ggaagacaga caattaacca
    10861 ttggctttgt gccatcggcg gaagtgaatt tattgccaaa agtattaccg atgtttcgtc
    10921 tcagacagcc agacaccttg attgagctgg tgagtttaat caccacgcaa caggaggaaa
    10981 aaattcgccg tggtgaactc gacgtcggct taatgcgcca tcctgtttat agcccggaga
    11041 ttgattatct ggagcttttt gacgaaccat tagtggtggt gttaccggtt gatcaccctt
    11101 tagcacatga aaaagagatc accgccgcgc aactggatgg cgtgaatttc gtcagtaccg
    11161 atccggcgta ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca
    11221 gccagccaaa tatcgtccag gtggcaacga atattctggt gaccatgaat ctggtgggca
    11281 tggggctggg cgtcactttg atacccggtt atatgaataa ttttaatacc gggcaggttg
    11341 tttttcgtcc cattgccgga aatgtgcctt ccattgcttt actgatggcg tggaagaaag
    11401 gggagatgaa gccggcatta cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg
    11461 taacggcata attacgcatt atttttgcaa catgcgccgc ggatcgcagt tttttagtgc
    11521 ttgtgaactg gcgttacaga catcgggaag caggctcgaa tattcttatt tttcaacttg
    11581 caccaacatt cgattaaccc cagactaacg cctcctgacg ggagggactc atggttttgc
    11641 aatccacgcg ctggttggcg ctcggctatt tcacatactt ttttagttac ggcatttttt
    11701 tacctttctg gagcgtctgg cttaaaggga ttggtttaac gccagaaacc atcggcctgt
    11761 tattgggggc aggtctggtt gcccgttttc tcgggagttt gctcatcgcg ccccgcgtca
    11821 gcgatccttc ccgcctgatt tccgccttgc gcgtgctggc actgctgcca cttctctttg
    11881 ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg attggcttta
    11941 acctcttttt ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc
    12001 agttcccgct tgattacggc aaagtgcgac tgtggggctc ggtggcgttt gtcattggct
    12061 cggcgctgac gggcaaactg gtcactatgt ttgattatcg ggtgatcctc gtgctgttga
    12121 cgttgggcgt ggcatccatg ctgctcggct ttctcatccg tccgacgatt cagccacaag
    12181 gggcaagccg ccagcaggag agcaccggtt ggtctgcgtg gttggcgctg gttcgccaga
    12241 actggcgctt tctggcctgc gtttgtttat tgcagggggc acatgcggcc tattacggtt
    12301 ttagcgccat ttactggcag gcagctggct actcggcctc ggcggtgggg tatttgtggt
    12361 cgctgggcgt ggtggcggaa gtcattatct ttgcgctgag taataaactt ttccgccgtt
    12421 gtagtgctcg cgatatgctg ttgatctcgg cgatttgcgg cgtagtgcgc tggggcatta
    12481 tgggagcaac tacggcgttg ccgtggttga tagtggtgca aattctgcat tgcggcacct
    12541 tcacggtctg ccacctggcc gccatgcgtt atattgctgc tcgccaggtt agcgaagtca
    12601 tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg cagtatcgct atcatgaccg
    12661 ttttcgccgg tttcctgtat caatatctgg gccacggtgt gttctgggta atggcgctgg
    12721 tggcgcttcc ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt
    12781 ttgcggatct gctcctgttg atgtgttgtc agcgtcaggt ccgtatagat cagcggtggt
    12841 gaaaagagcg gtaacggcat ggtataaggc gcaataatca gtgccgcgcc acgcggagcg
    12901 cctgtctgca aaaatgcctt taccgacata tgcttaatgt tgagcggcag taacgttagt
    12961 tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat cattcccggt cagtagaata
    13021 atctgttttt catgcagatc gttttcctgc attagccagg caccgaaaat caccgcgacc
    13081 agaccacttt cctcatcaga aagatggacg ccgtattcac tttcaaatcc ggccagcacc
    13141 gcgcgggtgg tgcgtaccag gcgtggatac agtctggcga actcttccgg cagggtatta
    13201 tcaataccga tggcaaacaa actgcgattt aacgcctggg cgaggtggat ataaagctga
    13261 tcacataacc cctgttcatc ataaaaacga acatttccca gctcacgaaa atggtttacc
    13321 agacgtttga tagactgacg caattgtctg tcccgctgat gcgcatcccg cagtggatcg
    13381 ggaacccgca acatggaaaa aagtaatgcc ataaacagtg gctcatcagg cggtacatgc
    13441 tggagcgccc gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag
    13501 gattccgccc agcgacgttg gagcggatta aactgaggcg taattccagc gtggtgctgc
    13561 agcaaacaat attgcagata cagacacagg aaatgaatat cgcgcgattc aaagtgtttt
    13621 tgcagccgtc gggagcagag attcactaac gcttgtagat tggtatcgtc gtaaaagtta
    13681 cgcgcaattc cgcgccgctt cagttcactc ttcagggcgg gggtgaattg gctggtaata
    13741 aatgacggac acagacggaa accacgtcgt agccagtgaa ataaacacaa acgttggtta
    13801 agcactgtac cttcaacccg gtagctaccg tcataaccag tcgtcagtgt gagttgatga
    13861 tagcgcagga tctcccgtcc tgtttcgctg atatcaagac tggcaatatc atcatccaca
    13921 ccattaagct cgctgaaggt tgccatggtg gcggtcaacc ccggctgaaa gagcgtcagc
    13981 aagatctggc agcggcgctg gggagccgaa aggacagatg gtggagcaag cgtaggcatc
    14041 atctgtagat ctcccataag cataattgat aagaatagct aaagtttgct cagggaaatg
    14101 gcgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt tctcatttta
    14161 aactgttctg accagtggat tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct
    14221 agcgatcatg ggttagttct gcggattcaa cgcgctaagg ataaagtcca ccatctgttc
    14281 gcgataaaca ttgccgtgac gatcggaaaa agcatcaatg tgttcgccat ccgggattag
    14341 gatcaaccgt tttggctctt ttgccaggct atacaacttt tcgctgtgct gccatgggat
    14401 aacgtgatcg gctttaccgt gaatgagtaa aagcgggatc gggctgacgc tggcgatgta
    14461 attttcgccg ctgtaactct catcaagtaa gtagccactg ccggggatca tctggttggc
    14521 gatggttgca taagaggcaa atgtggagtc gaggatcacc gcacgtatgc cttcacgatc
    14581 accctgacca ataacagcaa gaatattcgc cccgccaata ctctgcccga acagcaccag
    14641 ccgttgtggg tttacatcac tgcgatggcg caccacattg atggcgcttt gcgtatcgtc
    14701 cagcaatccg gcctgggacg gagtgccttt tgatttacca aacccgcgat aatcaaacat
    14761 aaaaacgttg aaattacgct caggtaacca actgaccagc ggccagtggg cggacatatt
    14821 tccggcattg ccgtgagcat gaatgatggt tgcgatggcg ttgtcagcag ggctcgtcga
    14881 agaaggaata aaccagcctt gcagacgagt accgtcctta gccgtaaatt cgacggattc
    14941 cgccgaccag gggtcgggac cgtaaatttt gtcgtcagga taatagaaga agttgatggc
    15001 aacgcgcagc actaaataaa cgcagaaggc aacaacaaag agaataaaca gaattttggg
    15061 aacgcgtttg ttcactggca gtgccataac ctttccttgc gacttcttta tcatccatta
    15121 atcggggaaa gatggtggga gagtgacgaa agaaagtaaa gcggtgagga agtatttcgg
    15181 aatagcgcct gaaaggcgag ggcgggtgag tgatatcacc cgcctgagtc attaacgctt
    15241 cagagcgtcg cttaactcgt cacgcatgtt cgccagtatg gctttcacaa cacgcgggtt
    15301 accagcaacg atgttaccgg tcagcatata gttatgacca ccggtgaagt cgctgacgat
    15361 gccgcccgct tcacgaacca gcagctcgcc tgccgcgaaa tcccacggac gcagaccgat
    15421 ttcaaagaaa ccgtcaacac gacccgcagc gacgtaagcc agatccagcg ccgcagaacc
    15481 ggtacgacgg aagtctgcac attcgttgaa cagcttgccg acgatgttga tgtaggtagt
    15541 ggcgtactgt tttgctttga acgggaagcc ggtcgccaga atggtaccgt cgagatcgcg
    15601 agcggtgcta ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt
    15661 gaacagttcg ttacgcatag gatcgtatac cacagcaact tcggtgcggc ctttgatacg
    15721 aacggcgata gataccgcga agtgcggcag acgtttgata aagttggtag taccatccag
    15781 tggatcgata acccattgaa catcctgatc agtaccttca agttcaccgc tttcttcggt
    15841 gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg cttcggcagc
    15901 tttatctacg ttggtcacga aatcgttact gcctttctgg ctcgcttcta cagcgtccgg
    15961 ggtttcatag tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt
    16021 cagcatcgga tgcatcggtc tctctcactg gatgttaaag aacgggaaaa cggcgcagag
    16081 tatagcagcg agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa taatctttag
    16141 atgaagaaag acaatgctgc aaaatattcg aattgtgctg gtggagacgt cacacaccgg
    16201 caatatgggt tctgttgccc gtgccatgaa aacaatggga ttaaccaatc tgtggctggt
    16261 taatccactg gtgaaacccg actcccaggc gattgccctg gctgcagggg ccagcgatgt
    16321 gattggtaat gctcacatcg tcgatacgct cgacgaagcg ttagccggtt gtagcctggt
    16381 ggtgggcacc agcgcacgct cccgcacgct gccgtggccg atgctcgacc cgcgcgaatg
    16441 cggtctgaaa agcgtcgctg aagcggcaaa taccccggtg gcgctggtgt ttggtcgtga
    16501 gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat tatcatgtcg cgattgcggc
    16561 aaacccggaa tacagttcgc tgaacctggc aatggcggtt caggttattg cctatgaagt
    16621 gcgtatggcc tggctggcaa ctcaggaaaa tggcgagcag gtcgagcatg aagagacgcc
    16681 gtatccgctg gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa cgctgctggc
    16741 aaccggtttt atccgtgaaa accatccggg gcaggtgatg aataaattgc gccgtctgtt
    16801 tacccgtgcg cgcccggaaa gccaggaatt gaatatcctg cgcgggattc tggcttctat
    16861 tgagcagcag aataaaggta acaaggccga ataacagtgg ttgcctgatg cgacgcttaa
    16921 cgcgtcttat caggcctaca atgtacagaa tcgtaggtcg gataaggtgt tcacaccgca
    16981 tccgacaaca ggtacaaacg ccacgataaa aaaatggcac tgaaggttaa atacccgact
    17041 aaatcagtca agtaaatagt tgaccaattt actcgggaat gtcagacttg accctgctat
    17101 gcaatacccc cacttttaca ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa
    17161 gacatgagac tgacatctaa agggcgctat gccgtgaccg caatgcttga cgttgcgctc
    17221 aactctgaag cgggcccggt accgttggct gatatttccg aacgtcaggg aatttccctt
    17281 tcttatctgg aacaactgtt ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt
    17341 ggaccaggcg gtggttatct gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg
    17401 attagcgccg ttgacgaatc tgtagatgcc acccgttgtc agggtaaagg cggctgccag
    17461 ggcggcgata aatgcctgac ccacgcgctg tggcgtgatt tgagcgaccg tctcaccggt
    17521 tttctcaaca acattacttt aggcgaactg gttaataacc aggaagtgct ggatgtgtct
    17581 ggtcgtcagc atactcacga cgcgccacgc acccgcacac aagacgcgat cgacgttaag
    17641 ttacgcgctt aataaaaaga attcagaatc aggccggagt gctaaatact ccgtaaacac
    17701 ggtcgtacat ccagccggta gcctgattcc ttgcattgag tgatgtacgg agtttataga
    17761 gcaatgaaat taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt
    17821 gccgagaaaa tgatgcagtt catgacgatg gacggaacct ttggtaaccc ggcctctcgt
    17881 tctcaccgtt tcggctggca ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc
    17941 gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac
    18001 aacctggcga tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc
    18061 agcaaaaccg aacacaaagc ggtactggat acttgccgtc agctggagcg cgaaggtttc
    18121 gaagtcacct acctggcacc gcagcgtaac ggtattatcg acctgaaaga actcgaagcg
    18181 gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg
    18241 gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt
    18301 gatgcaaccc agagcgtggg caaactgcca atcgacctga gccagttgaa agttgacctg
    18361 atgtctttct ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct gtatgtgcgt
    18421 cgtaaaccgc gtgtacgcat cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg
    18481 cgttccggca ctctgcctgt tcaccagatc gtcggaatgg gcgaagccta tcgcatcgca
    18541 aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac
    18601 ggcatcaaag atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac
    18661 attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac
    18721 ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg
    18781 cgcgcgctgg ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt
    18841 tttactactg aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt
    18901 ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc
    18961 gaatgggctc atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa
    19021 aaagttatcg accattacga gaatccgcgt aacgtgggtt cctttgataa caacgacgag
    19081 aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg acgtaatgaa gttgcagatt
    19141 aaagtcaacg atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttcc
    19201 gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg
    19261 caggcgatca aaaacaccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac
    19321 tgttctattc tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt
    19381 gaagcaaaat aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc
    19441 gcgagtaaat acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag
    19501 aacctccgga tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga
    19561 agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt
    19621 tctggacggt acgcagctgg acttcgtaaa agaaggcctg aatgaagggt ttaaattcac
    19681 caacccgaac gttaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata
    19741 ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc
    19801 gcggcggatc gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc
    19861 cgttatcaac tcgataccca ggcactgagc ctgcgttttc aggatctaca acgtcagtat
    19921 catcctgata aattcgccag cggaagcctg gcggaacaac tcgccgccgt acagcaatct
    19981 gcaaccatta accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg
    20041 ctttctttac acggttttga tctcgccagc gagcagcata ctgtgcgcga caccgcgttc
    20101 ctgatggaac agttggagct gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa
    20161 gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg
    20221 atggttgaac agttagacaa cgaggcgtgg gacgcggcgg cggatacggt gcgtaagctg
    20281 cgttttctcg ataaactgcg gagcagtgcc gaacaactcg aagaaaaact gctcgatttt
    20341 taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc
    20401 gccgcatcag cgtcgtctgg cggctggtat tgacctgggc acaaccaact cgctggtggc
    20461 gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc
    20521 atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc gtactaacgc
    20581 agcgcttgac accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc
    20641 tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg aaaacggcct
    20701 gccgatgatt gaaacggcgg cggggctact gaacccggtg cgcgtttctg cggacatcct
    20761 caaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat
    20821 caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct
    20881 ggcggggttg catgttctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg
    20941 gctggattcc ggtcaggaag gcgtgatcgc tgtttatgac ctcggtggcg ggacgtttga
    21001 tatttccatt ctgcgcttaa gtcgcggcgt gtttgaagtg ctggcaaccg gcggtgattc
    21061 cgcgctcggc ggcgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcgga
    21121 cattcctgat cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc
    21181 caaaatcgcg ctgagcgatg cggactccgt gaccgttaac gttgcgggct ggcagggcga
    21241 aatcagccgt gaacaattca atgaactgat cgcgccactg gtaaaacgaa ccttactggc
    21301 ttgtcgtcgc gcgctgaaag acgcgggtgt agaagctgac gaagtgctgg aagtggtgat
    21361 ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg
    21421 tccaccgctg acttccatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc
    21481 ggatattctg gtgggtaaca agccagacag cgaaatgctg ctgcttgatg tgatcccact
    21541 gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac
    21601 tattccggtg gcccgcgctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc
    21661 tatccatgta atgcagggtg agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt
    21721 tgcgctgcgt ggtattccgg cgctaccggc tggcggtgcg catattcgcg tgacgttcca
    21781 ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggag aaatccaccg gcgttgaggc
    21841 gtctattcag gtcaaaccgt cttacggtct gaccgatagc gaaatcgctt cgatgatcaa
    21901 agactcaatg agctatgccg agcaggacgt aaaagcccgt atgctggcag aacaaaaagt
    21961 agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt
    22021 aagcgccgca gagcgtcagg tcattgacaa tgctgccgct cacctgagtg aagtggcgca
    22081 gggcgatgat gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga
    22141 tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga
    22201 cgaggtttaa tatgccaaag attgttattt tgcctcatca ggacctctgc cctgatggcg
    22261 ctgttctgga agctaatagc ggtgaaacca ttctcgacgc agcgctgcgt aacggtatcg
    22321 agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg
    22381 aaggttttga ctcactgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct
    22441 ggggactgga gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac gaagatttag
    22501 tggtcgaaat cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat
    22561 gggacttaag tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga
    22621 tcttgatccg aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga
    22681 tttcgacgac gacccgcagg catccaacga gaaaatcctc gaagcgattt tgttagtctg
    22741 gctggacgaa gccgagtgaa tgaacgggct gcctgggggt agcccgtttt gctgttaaac
    22801 gaataaggat aactaaaatg acagaagcga tgaagattac catctctacc caacctgccg
    22861 acgcgcgctg gggagaaaaa gcaacttaca gcattaataa tgacggcatt accctgcatt
    22921 tgaacggggc agacgatctg gggctgatcc agcgtgcggc ccgcaagatt gacggtctgg
    22981 gcatcaagca tgttcagtta agcggtgaag gctgggatgc ggatcgctgc tgggcattct
    23041 ggcaaggtta taaaggcccg aaaggcacgc gtaaagtgga gtggccggat ctggacgatg
    23101 cccagcgcca ggaactggat aaccgcctga tgatcatcga ctgggtgcgt gacaccatca
    23161 acgcaccggc agaagaattg ggaccatcgc aactggcaca gcgtgctgtt gatctgatca
    23221 gcaacgtcgc gggcgatcgt gtgacttatc ggatcaccaa aggcgaagat ctgcgtgagc
    23281 aaggttatat ggggctgcac actgtcgggc gcggttcaga acgttctccg gtattgctgg
    23341 cgctggatta caacccaact ggcgataaag aagcaccggt gtacgcgtgc ctggtaggta
    23401 aaggtatcac ttttgactcc ggcggctaca gcatcaaaca gactgcgttt atggactcga
    23461 tgaagtcgga catgggcggc gcggcaacgg ttaccggggc gctggcattt gccattacgc
    23521 gcggactgaa caagcgcgtg aagctgttcc tctgctgtgc ggataacctg attagcggca
    23581 atgcgttcaa gctgggcgat atcatcacct atcgcaacgg taaaaaagtt gaagtgatga
    23641 acactgatgc cgaagggcgt ctggtgcttg ccgatggtct gattgatgcc agtgcgcaga
    23701 aaccggaaat gatcattgat gcggcgaccc tcaccggggc ggcgaaaact gcgctgggta
    23761 atgattatca cgcgctgttc agttttgacg atgcgcttgc cggtcgttta ctggcgagtg
    23821 cctcacaaga gaacgaacca ttctggcgtc tgccgctggc ggaattccac cgcagccagc
    23881 tgccgtctaa ctttgccgaa ctgaacaata ccggaagcgc ggcgtatccg gcaggcgcga
    23941 gcacggcggc gggcttcctg tcgcactttg ttgagaacta tcagcaaggc tggctgcata
    24001 tcgactgctc ggcgacttac cgtaaagcgc cggttgaaca gtggtctgcg ggcgctacgg
    24061 gacttggtgt gcgcacgata gctaatctgt taacggcgta aactaatatg ccggatgcgg
    24121 cgtaaacgcc ttatccggcc tacaaattct gcaaattcaa aaaattgcga atcccttgta
    24181 ggcctgataa gcgtagcgca tcaggcattt gcgccccgaa cgttgaatga attgtggttc
    24241 ttatgtccga aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg
    24301 cgcaccgccc ggcctttttc cgtactctac tggaatccac cgtctgggtg cctggtacgg
    24361 cggcgcaggg cgaggctgtg gttgaagata gtgcgcttga tttacagcac tgggaaaaag
    24421 aagacggcac cagcgtcatt ccttttttca cctcgttaga agcgcttcaa caggcggttg
    24481 aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg
    24541 aaacgctctt ccttaatgcc aaactgccga ccggtaaaga atttatgccg cgtgaaatca
    24601 gtttgttgat tggtgaagag ggaaatccgc tgagcagcca ggaaatcctg gaaggcggtg
    24661 aatcgctgat attatcggaa gtcgcagagc cgccagcaca aatgattgat tcactcacca
    24721 ccttatttaa aaccattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg
    24781 aagaggcaca gcctaattta cttattggca ttgaagccga tggtgatatc gaagaaatta
    24841 ttcaggcgac gggaagtgta gcgaccgata cattacctgg cgatgaacca atcgatattt
    24901 gtcaggtgaa aaaaggggaa aaaggaatta gccactttat taccgaacat attgcgccat
    24961 tctatgaacg tcgctggggt ggttttttgc gtgactttaa acaaaaccgg ataatctaaa
    25021 atatgccgcc ctaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccac
    25081 cagtaaattg tcgccggaga gactccggtt attatttact tgggaaaaca gcgcaccaga
    25141 aacaataagt acgatacaga agcaccgtat cttcattgtg ctgtcgtgat gcgaagaccg
    25201 catcacgaca gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc
    25261 taacggtcgc ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg
    25321 ccgcagtgga taatacacta acagaacaat aataactctc ttttgcttga caaaaagaga
    25381 gttactggcg agtattgtct ccgctgagac gcgcttgcat tactacggta atacaacctg
    25441 ctggaaaggc cgcgaaccgg accagcaaca aataaccgcc tgattttggc ggttttttgt
    25501 tttttaaagg aaagtaaatt tacatgttta agtaatatct tttaccggtt agaattttta
    25561 attttgagca ggatttgttt atatttgtct gaattcggac taataagcca ggttatttta
    25621 ttaccgaaca cggcggaaag ggccggtgtc gataatacgc taaataaaca ataattactc
    25681 tcttttgctt gacaaaaaga gagttactgg tgagtattgt tttactgtga agcacctgcg
    25741 ttgctcatgt aacacaagtc aacctgctgg aaaggccgcg aaccagacca gcaataaaaa
    25801 aacgccaaat tcggcggttt tttattgcct gtaaaatact tatttcactg gctcaaccgg
    25861 taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg tacagtttca cgtttggcac
    25921 atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt acaccagagc cgcagctgac
    25981 gataatcggt ttgtcatagc tgacgccgcg accaaaaaat atcgcatcca gttcatcggt
    26041 cgtttttagt tcgccttcgc gcaccagttc cgtccacgga acgttcaatg caccgggaat
    26101 atgtccgcga cgtaaacctg ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg
    26161 gcgggcatca ataatttgcg ccgtattttc atggcttgcc aataacacat cggttacttt
    26221 caccactgct tcaggattaa acgcggcgtt aaactctcct tccggcagct ctactgcacc
    26281 ttcttctaat agcaggtcat cgcgctgcca gcctgcaagt ccccgttact ttattcgtac
    26341 cccttataat ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtccat
    26401 gatccggggg g
//
LOCUS       DADLZM010000045        25943 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLZM010000045 DADLZM010000000
VERSION     DADLZM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.045"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..188
                     /locus_tag="JNY73_001840"
     CDS             <1..188
                     /locus_tag="JNY73_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8047870.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNY73_001841"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNY73_001841"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY8047871.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /locus_tag="JNY73_001842"
     CDS             1156..3192
                     /locus_tag="JNY73_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY8047872.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="JNY73_001843"
     CDS             3150..3545
                     /locus_tag="JNY73_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY8047873.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="JNY73_001844"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="JNY73_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY8047874.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="JNY73_001845"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="JNY73_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY8047875.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="JNY73_001846"
     CDS             complement(4854..5894)
                     /locus_tag="JNY73_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY8047876.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="JNY73_001847"
     CDS             complement(5967..6602)
                     /locus_tag="JNY73_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY8047877.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="JNY73_001848"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="JNY73_001848"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY8047878.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTSAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="JNY73_001849"
     CDS             complement(7228..7671)
                     /locus_tag="JNY73_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY8047879.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNY73_001850"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNY73_001850"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY8047880.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGVAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="JNY73_001851"
     CDS             complement(8011..8514)
                     /locus_tag="JNY73_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8047881.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9032)
                     /locus_tag="JNY73_001852"
     CDS             complement(8508..9032)
                     /locus_tag="JNY73_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY8047882.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9241..10236
                     /locus_tag="JNY73_001853"
     CDS             9241..10236
                     /locus_tag="JNY73_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY8047883.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10245..11123
                     /locus_tag="JNY73_001854"
     CDS             10245..11123
                     /locus_tag="JNY73_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY8047884.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11204..12211
                     /locus_tag="JNY73_001855"
     CDS             11204..12211
                     /locus_tag="JNY73_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY8047885.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSIQQAFVKLRRGEIRQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNY73_001856"
     CDS             complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNY73_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY8047886.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13727..15616)
                     /locus_tag="JNY73_001857"
     CDS             complement(13727..15616)
                     /locus_tag="JNY73_001857"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY8047887.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNY73_001858"
     CDS             complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNY73_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY8047888.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNY73_001859"
     CDS             complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNY73_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY8047889.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNY73_001860"
     CDS             complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNY73_001860"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY8047890.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNY73_001861"
     CDS             complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNY73_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY8047891.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNY73_001862"
     CDS             complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNY73_001862"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY8047892.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNY73_001863"
     CDS             complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNY73_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY8047893.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNY73_001864"
     CDS             complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNY73_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY8047894.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNY73_001865"
     CDS             complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNY73_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY8047895.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNY73_001866"
     CDS             complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNY73_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY8047896.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6130 a         7217 c         6541 g         6055 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aattagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aacctgtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgtcagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca ggggttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac
     8881 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
     8941 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
     9001 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
     9061 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
     9121 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
     9181 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
     9241 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
     9301 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
     9361 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
     9421 cttcacatct cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
     9481 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
     9541 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
     9601 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
     9661 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
     9721 gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg
     9781 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
     9841 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
     9901 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
     9961 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    10021 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    10081 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    10141 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    10201 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    10261 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    10321 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    10381 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    10441 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    10501 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    10561 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    10621 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    10681 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    10741 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    10801 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    10861 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    10921 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    10981 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    11041 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    11101 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    11161 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    11221 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    11281 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    11341 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    11401 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    11461 ctcaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    11521 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    11581 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    11641 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    11701 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    11761 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    11821 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    11881 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatac agcaagcctt
    11941 tgtgaagctg cgccgtggcg aaatcaggca actgccgccg ccgattcaaa atatggatca
    12001 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    12061 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    12121 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    12181 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    12241 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    12301 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    12361 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    12421 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    12481 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    12541 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    12601 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    12661 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    12721 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat
    12781 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    12841 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    12901 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    12961 acgacgccaa gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    13021 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    13081 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    13141 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    13201 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    13261 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    13321 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    13381 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    13441 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    13501 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    13561 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    13621 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    13681 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    13741 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    13801 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    13861 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat
    13921 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    13981 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    14041 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    14101 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    14161 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    14221 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    14281 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    14341 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    14401 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    14461 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    14521 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    14581 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    14641 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    14701 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    14761 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    14821 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    14881 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    14941 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    15001 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    15061 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    15121 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    15181 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    15241 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    15301 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    15361 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    15421 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    15481 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    15541 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    15601 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    15661 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    15721 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    15781 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    15841 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    15901 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    15961 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc
    16021 cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt
    16081 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg
    16141 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    16201 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    16261 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    16321 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    16381 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    16441 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    16501 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    16561 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    16621 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    16681 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    16741 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    16801 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    16861 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    16921 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    16981 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    17041 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    17101 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    17161 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    17221 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga
    17281 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    17341 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    17401 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    17461 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    17521 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    17581 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    17641 cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    17701 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    17761 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    17821 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    17881 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    17941 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    18001 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    18061 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    18121 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    18181 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    18241 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    18301 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    18361 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    18421 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    18481 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    18541 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    18601 gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt
    18661 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    18721 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    18781 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    18841 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    18901 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    18961 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    19021 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    19081 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    19141 tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    19201 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    19261 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    19321 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    19381 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    19441 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    19501 gtacgttacg caaagtgtta aataatttac gcgacgttaa gcggcagtat tctactcgta
    19561 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    19621 gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt
    19681 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    19741 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    19801 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    19861 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    19921 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    19981 caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc
    20041 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    20101 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    20161 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    20221 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    20281 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    20341 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    20401 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    20461 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    20521 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    20581 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    20641 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    20701 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    20761 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    20821 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    20881 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    20941 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    21001 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    21061 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    21121 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    21181 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    21241 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    21301 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    21361 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    21421 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    21481 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    21541 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    21601 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    21661 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    21721 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    21781 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    21841 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    21901 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    21961 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    22021 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    22081 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    22141 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    22201 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    22261 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    22321 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    22381 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcagcgtgc cccggggtgt
    22441 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa
    22501 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    22561 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    22621 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    22681 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    22741 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    22801 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    22861 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    22921 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    22981 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    23041 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    23101 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    23161 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    23221 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    23281 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    23341 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    23401 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    23461 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    23521 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    23581 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    23641 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    23701 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    23761 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    23821 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    23881 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    23941 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    24001 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    24061 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    24121 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    24181 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    24241 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    24301 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    24361 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    24421 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    24481 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    24541 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    24601 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    24661 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    24721 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    24781 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    24841 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    24901 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    24961 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    25021 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    25081 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    25141 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    25201 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    25261 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    25321 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    25381 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    25441 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    25501 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    25561 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    25621 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    25681 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    25741 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    25801 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    25861 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    25921 ttgaaaaagg actctaaggg aaa
//
LOCUS       DADLZM010000046        25444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLZM010000046 DADLZM010000000
VERSION     DADLZM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.046"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            273..614
                     /locus_tag="JNY73_001867"
                     /pseudo
     CDS             273..614
                     /locus_tag="JNY73_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="JNY73_001868"
     CDS             complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="JNY73_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY8047897.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            2626..3186
                     /locus_tag="JNY73_001869"
     CDS             2626..3186
                     /locus_tag="JNY73_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY8047898.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            3361..3570
                     /gene="cspE"
                     /locus_tag="JNY73_001870"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             3361..3570
                     /gene="cspE"
                     /locus_tag="JNY73_001870"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY8047899.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="JNY73_001871"
     CDS             complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="JNY73_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY8047900.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            4100..4888
                     /locus_tag="JNY73_001872"
     CDS             4100..4888
                     /locus_tag="JNY73_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY8047901.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTLGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            5017..5220
                     /gene="tatE"
                     /locus_tag="JNY73_001873"
     CDS             5017..5220
                     /gene="tatE"
                     /locus_tag="JNY73_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY8047902.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="JNY73_001874"
     CDS             complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="JNY73_001874"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY8047903.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(6495..7448)
                     /locus_tag="JNY73_001875"
     CDS             complement(6495..7448)
                     /locus_tag="JNY73_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY8047904.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQSVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="JNY73_001876"
     CDS             complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="JNY73_001876"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY8047905.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            complement(8449..8712)
                     /locus_tag="JNY73_001877"
     CDS             complement(8449..8712)
                     /locus_tag="JNY73_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY8047906.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="JNY73_001878"
     CDS             complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="JNY73_001878"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY8047907.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="JNY73_001879"
     CDS             complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="JNY73_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY8047908.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="JNY73_001880"
                     /gene_synonym="rodA"
     CDS             complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="JNY73_001880"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY8047909.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="JNY73_001881"
     CDS             complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="JNY73_001881"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY8047910.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="JNY73_001882"
     CDS             complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="JNY73_001882"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY8047911.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="JNY73_001883"
                     /gene_synonym="rsfA"
     CDS             complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="JNY73_001883"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY8047912.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            complement(15367..15978)
                     /locus_tag="JNY73_001884"
     CDS             complement(15367..15978)
                     /locus_tag="JNY73_001884"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY8047913.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="JNY73_001885"
     CDS             complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="JNY73_001885"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY8047914.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            complement(16645..17676)
                     /gene="holA"
                     /locus_tag="JNY73_001886"
     CDS             complement(16645..17676)
                     /gene="holA"
                     /locus_tag="JNY73_001886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY8047915.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="JNY73_001887"
     CDS             complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="JNY73_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY8047916.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="JNY73_001888"
     CDS             complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="JNY73_001888"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY8047917.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            21089..21571
                     /locus_tag="JNY73_001889"
     CDS             21089..21571
                     /locus_tag="JNY73_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY8047918.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTELDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            complement(21641..22624)
                     /locus_tag="JNY73_001890"
     CDS             complement(21641..22624)
                     /locus_tag="JNY73_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY8047919.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            22782..23489
                     /locus_tag="JNY73_001891"
     CDS             22782..23489
                     /locus_tag="JNY73_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8047920.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            23486..24913
                     /locus_tag="JNY73_001892"
     CDS             23486..24913
                     /locus_tag="JNY73_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY8047921.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(24923..25381)
                     /locus_tag="JNY73_001893"
     CDS             complement(24923..25381)
                     /locus_tag="JNY73_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8047922.1"
                     /translation="MVNYAFDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGEFYLHG
                     SDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQG
                     YAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
BASE COUNT         6357 a         6253 c         6322 g         6512 t
ORIGIN      
        1 atgagctgat ttgtgatatg agtcatttgg tgacagcctg gttgttatcg gttttgaacg
       61 ttttttgaag ctatgattga aatgatttat agtgtttttg tttttcgttt tttcaatgaa
      121 acaaaagcaa ttttttacta tctttcgcgt ttcattggtt aacgcctaaa atttgtacta
      181 caggcttgca agccccacca aatctaatcc catcagatcc cgaataatcc aatgttctcc
      241 cggttttttc gtacttcaag tgaatcaata cactggggca aacgctaacc cgtttccgcc
      301 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
      361 ttaatgccta tgctcgcggt gaacccaaag acgaactgcc gaccggcatt gaccccttga
      421 cgctaaacgc ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg
      481 tggcgcaggc actgaccatc agccgcacca ctgccaggcg ttatcttgaa tattgcgcca
      541 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagacca caacgcatat
      601 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
      661 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
      721 tagcccaaca agcaccggca ccgaggtgcg ttttacgact tcaaatggtg agattttcgc
      781 catcccggca accgcaacga ctacgccaga aacgggtgat agggtacgac ccaggtttga
      841 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
      901 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
      961 cagcatggtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     1021 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     1081 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccatcccgc gataagccac
     1141 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     1201 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc
     1261 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg cgggggcagt
     1321 ggtggtgatt tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata
     1381 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     1441 cgtcgttttg aaggcgaagt caatcagcga catttcggaa gcctgcgccg ccagcaccac
     1501 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagctgctgc
     1561 gccacgactg ataccaacgt ttaccatcac cggaaacagg gttgccatca gcaaaacacc
     1621 cagaccggtt gcggaagaga cggccagtga catcagacaa gcaacaaaat aggcggcaat
     1681 catcagcagg taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat
     1741 atcattcgcg ccgatatggg tcatgtaagc ggcaaagcca cacagcatca taatcatcat
     1801 gccgaggtcg ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc
     1861 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat
     1921 aatcagcaat aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat
     1981 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     2041 aatttttcct gtctccaggc ccaaagtaaa taataaaaac ttcctaaagc ttaaggaaaa
     2101 aatatgccca ataaattggc gatgaatgct gattaaaatc aagaaaaact gccattaaga
     2161 cattgaagtt gctgttttta tacatagata acaaaacgca tatttagcgg ttggcaatta
     2221 tcacataata atgtgtgtaa attgatggtg gattgatgca aatttgttaa ttgcaatggt
     2281 gttagctgcg gctgtgcgct caaaaaataa tctaatatga gcatgggttg acgatatata
     2341 ttatatactt cgttaagatg attgttgtat ctcgttaaaa aaaaataatt tttccttgat
     2401 tgcattttgt catcaaaaaa gacttggttt ttcttttttg actattccca tcgcagaaaa
     2461 cgacgcatca tctttaatcg atgcgcggaa atatttaact tgaacaagcg gaaataaata
     2521 gagcagctat tcagattatt ctttatgttg ggtctattaa ggttatgtta attgtagctt
     2581 tgctatgcta gtagtagatt tttgataaat gttttatggt cacaaatgaa cgtgagtaaa
     2641 tatgtcgcta tcttttcctt tgtttttatt cagttaatca gcgttggtaa agtttttgct
     2701 aacgcagatg agaggatgac aacgtttaga gaaaatattg cacaaacctg gcaacagcct
     2761 gaacattatg atttatatat tcctgccatc acctggcatg cacgtttcgc ttacgacaaa
     2821 gaaaaaaccg atcgctataa cgagcgaccg tggggtggcg gttttggcct gtcgcgttgg
     2881 gatgaaaaag gaaactggca tggcctgtat gccatggcat ttaaggactc gtggaacaaa
     2941 tgggaaccga ttgccggata cggatgggaa agtacctggc gaccgctggc ggatgaaaat
     3001 tttcatttag gtctgggatt caccgctggc gtaacggcac gcgataactg gaattacatc
     3061 cctctcccgg ttctactgcc attggcctcc gtgggttatg gcccagtgac ttttcagatg
     3121 acctacattc cgggtaccta caacaatggc aatgtgtact ttgcctggat gcgctttcag
     3181 ttttgaggca aatgaagttt tagtaacttc tttaaaatca ataactaaaa taagcaacat
     3241 caaaaataac gcgactttta tcactttttg gtaaagttgc actggacaaa gcgtaccaca
     3301 attggtgtac tgataaccga cacagcattt gtgtctattt ttcatgtaaa ggtaattttg
     3361 atgtctaaga ttaaaggtaa cgttaagtgg tttaatgagt ccaaaggatt cggtttcatt
     3421 actccggaag acggcagcaa agacgtgttc gtacacttct ctgcaatcca gactaatggt
     3481 tttaaaactc ttgctgaagg tcagcgcgta gagttcgaaa tcactaacgg tgccaaaggc
     3541 ccttctgctg caaacgtaat cgctctgtaa gatacgtcag caagaattca aaacccgctt
     3601 aatcagcggg ttttttttgg tctttagtgt gcggttgagg ccgaaaacag ccagaatgcc
     3661 agtgcggtca tggcaaaaga ccccagaagg ttgacgaaaa cgttcagtaa tgcccagcca
     3721 aagcggccct cttgtaacaa aaacaccact tctgccgaaa atgttgagaa ggttgttaga
     3781 ccgccacaaa atccggtggt gattaatact ttccacactg gatcaatgtt cgtcatcctg
     3841 ctgaaccagg cgaatcccat tcctatgatg aatgccccaa tcaggtttgc tgccagcgtc
     3901 cccaacggaa tcgcctgatg cagcgggtta aatcgcatac ttaacagcca tctcgctacg
     3961 cttcccgtac caccgccaat aaaaactgct aaaagaagtt gtaacactgc aaaatcctgc
     4021 tatttgattt gtatgagtga taagtgtaac gccgaataat cgtcgttggc gaattttacg
     4081 actctgacag gaggtggcaa tgctggttgc tgcaggacag tttgctgtta catctgtgtg
     4141 ggaaaagaac gctgagattt gtgcctcgtt gatggcgcag gcggcggaaa acgacgtatc
     4201 gctgtttgtc ctgccggaag cattgctggc gcgcgatgat catgatgcag atctatcggt
     4261 taaatcagca cagctgctgg aaggcgaatt cctcggacgt ttacggcgag aaagtaaacg
     4321 taacatgatg acgacaattc tgacgattca tcttccttca acgctggggc gcgcatggaa
     4381 tatgctggtg gcacttcagg caggaaacat cgtcgcccgt tatgccaaac tgcatctcta
     4441 tgatgcattt gccattcagg aatcacgcca tgttgatgct ggtaatgaaa tagctccgtt
     4501 actggaggtg gaagggatga aggtcggtct gatgacctgt tatgacttac gctttccaga
     4561 gctggcgctg gcacaggctt tacagggagc tgaaatcctg gtacttcctg ccgcctgggt
     4621 tcgtgggccg ctcaaagagc atcactggtc aacgttgctt gccgctcgtg cgctggatac
     4681 cacctgttat atggtggcgg cgggggagtg cgggaacaaa aatatcgggc aaagccggat
     4741 tatagatccc tttggcgtca ccattgcggc agcgtcagaa atgcctgcac tcattatggc
     4801 ggaagtgacg cccgaacgtg tgcgtcaggt gcgcgcgcaa ctgcccatct taaacaaccg
     4861 tcgctttgcg ccgccgcaat tattatgatg tttttttact cggcgcttga ttcaccttgt
     4921 tacagattgc tattgtgtgc gcgcgtcgaa tgaccgttaa tattctctgg tttttaaggc
     4981 gcgttctgtt gccggttata tgtcaagaag gtatctatgg gtgagattag tattaccaaa
     5041 ctgctggtag ttgcggcgct ggtcgttctg ctgtttggga ctaagaagtt acgtacgctg
     5101 ggcggagacc ttggagcggc cattaaaggg ttcaagaagg cgatgaatga tgacgatgct
     5161 gcggcgaaaa aaggcgcaga cgttgatctt caggctgaaa agctctctca taaagagtga
     5221 cgtggcgagc aggacgctcc ctcaatatct tgttcgatac aaaaaacccg cttcaaaaag
     5281 cgggtttttt atcagacaga tgtaagtaat tattacagga ttacttaact tccatccctt
     5341 tcgcctgcaa atcggcgtgg taagaagagc ggacaaacgg accgcatgca gcatgggtaa
     5401 agcccatcgc cagcgcttcg gctttcattt cgtcgaactc atccgggcta acgtaacgtt
     5461 gaaccggcag gtgatggcgg cttggctgca aatattgccc cagcgttaac atcgtcacac
     5521 catgacggcg caggtcgcgc attacctcaa taatttcttc attggtttca cccagaccca
     5581 ccatcagacc agacttggtc gggatttccg gatgcgcttc tttaaagcgt tccagcagct
     5641 tcagcgacca gttgtaatct gcgccaggcc gtacctgccg gtaaatacgc ggtacgtttt
     5701 ccaggttatg gttgaacaca tctgggggcg ttgcagtcag aatatccaga gcacgatcca
     5761 ttcgaccgcg gaaatccggc accagagttt caattttgat ttgcgggctt ttttcccgaa
     5821 tggcagtaat acaatccgca aagtgttggg caccgccatc gcgcaggtca tcacggtcaa
     5881 cggaggtgat aaccacataa cgcagcgcca tatcggcaat ggtctgcgcc agtttcactg
     5941 gttcattggc atcaggagct accgggcgac cgtgggcaac atcacagaac ggacaacggc
     6001 gggtacaaat agcgccgagg atcataaacg ttgctgtgcc gtggttgaag cattccgcca
     6061 ggttagggca ggaggcttcc tcgcagacag aatgcaggcc atttttgcgc attgcagctt
     6121 tgatgccctg gatacgtgta gagtccgctg gaagcttgat tttcatccat tccggcttgc
     6181 gcagcagggc ttcgcgctct gttgccacgt ttttaaccgg gataagggcc atcttatcgg
     6241 catcgcggta tttaacaccg cgttccatca caatgggttt actcatagcg tgcgtgttcc
     6301 agttgcgaat tacgaaggaa agcgtttcaa ttcaatagat tgttgtaatt atcaactatt
     6361 tttgaattaa cgactggcag tatatcattg aaacggacct gaaagcagcc aaagcggtcg
     6421 cgaaaatgta aaattgttgt tggattgtgc cattttatcg ttctgcactg tgatctggct
     6481 gtaacaatac tttttcaaaa tgcattacgg ataacatcta ttactccgtg caggatggga
     6541 tcacgcaggc tgaatttgtt gtagtggata gagaaatcta tttgctcctc atttaaggac
     6601 ggaaaaggca gtttttccag cggccagcaa cgactaaaca ggttataaaa acggctggga
     6661 ataatcgcta acatgtcact gttggcaacc agtgcagcga ttgtcaaaat gttgtagctg
     6721 gtgaaattga tttgccggtc cggaaacatc tcttgaactc tctgccgcag accgctgaaa
     6781 ttttgccctt ccggtaacag aagtacatgc gcagcgttgt cgatagtctc tctgtcatct
     6841 tctaaagaga gtagtggatt tcccttgcgg caaattaaca ccatattgtc ggtgaacaga
     6901 acatgatgtt gcaccgtacg attggtgcaa aacatgttat cgatgatgag atcggtttga
     6961 aactgactga gttggttttc cgcgtcgctg atgggcgggt tgcgcagtaa aagctgcgga
     7021 tagtgagttt taatcgcccg atagatgaca ggaaggacca gggctccgac cgagggagtg
     7081 gtcgcaatag ttatcgttcg ttgcttatca tagcttcctt cgatatccag cgcgccaaga
     7141 atggactcaa ggccctgact gatatactca tgtagatgca tcgcaaatgc ggtaggagtg
     7201 acactctggc ctttgcgaat aaacaatggg tcagggaata taacgcgcag tttctgaata
     7261 gactgactga ttgccgaggg ggtcagatta agcactttcg ctgcattaac gatcccttta
     7321 tgtacatata cagcttcaaa aatagtcaga aggttaagat caatatttcg taaggttgta
     7381 aatatttgtg gtttaccttc tgacgacttt ctgcttaaac agggttcaat ttgattatta
     7441 ctatccacgc acttactcca attttattca tggaaaaata atatttaaaa aattacaata
     7501 atcttatgtc taattggaac ggaacgcttt tgctcaccat aatcaactat ttcaataggt
     7561 taatcaatgg ggtgagttac ggagcaaggc gtggcaccgc atttctgcaa gtgataaaga
     7621 aaatatacaa acgcgaagga gatgtaaagc attagcaggt attatgagta atgacccagt
     7681 gtaaattggg ccattgatgt atggaattaa gcggtaatat attcgaagtc cggattgttt
     7741 agtagcgcta aaatattttc cagtaaacgt ggagcaatat tattagtcgt cgcttcgggt
     7801 ttccattgtg atattttagc catttccatt ccggcatacc cacaaggatt aatacgtaaa
     7861 aatggtgaaa gatccatatt gacgtttaat gccagaccgt ggaatgaaca accgcgtcga
     7921 attcgtaaac ccagtgagca aattttcttt tccccgacat agacaccagg cgcgtcagcc
     7981 cgaggatgcg cttctatacc cagttcagcc agggtattca ccactgtttg ctcaagcaag
     8041 gtcaccagtt cacgcacacc gagtttacgg cgtttcaggt taagcaacac atacatcacc
     8101 tgttgtcccg gcccgtgata agtcacctgc ccaccgcgat cgctctggat caccggaata
     8161 tcacccggca ttaaaatgtg ctccgctttt cctgcctgac cttgggtgaa taccggatag
     8221 tgctcgacca gccagatttc atcaagggta ctattatcgc gggtatcggt gaattcatgc
     8281 atagcccggg agattggctc gtaaggctga agaccgagct ggcggacaag aattttatcc
     8341 tgatacaaaa cggcatctcc gtggagaatg agtaaaagtg ggggaaaagt atatcacagc
     8401 gaggagagga gagttacccg accaggagcc gggtaacgga gaagcgagtt acagaaccat
     8461 gcggacaata tcgattttgc ccagttcttc atacagtgtt tcaacctgct cgatatgagt
     8521 ggcgttgata gtgatagata ccgagtggta gttgcctttg ctgcttggtt ttaccgttgg
     8581 ggtgtagtca cctggcgcat ggcgctgtac cacttcaacc acctgatcaa ccagctcagg
     8641 taacgcctgc cccataactt tgtaagtaaa aggagtaggg aattcaagca gttcgttaag
     8701 tttggttttc atgtcagctc cggcgtaacg taattaaata gcaactcccg ccagaaggtg
     8761 ggagtttttt tactgatgct tagtatatgg ggacggaaat tacactttca agtgttcaat
     8821 ttttaaccaa accagtgatg gaacattaat ttaatgtaat caatgatttt gccgaagaag
     8881 ttaccttccg ggatttcttg caacacaacc agcgggcgtt gctcgatcgt tttgccatca
     8941 agctggaagt tgatagttcc gacgacctga ttcttttgca gcggcgcatg caattcactg
     9001 ctgttcagca catagctggc tttcagatct ttcatgcgac cacgcggaat ggtcaggtac
     9061 acgtctttat caacccctaa cgaagcgcga tcagaatcac taaaccaaac cggttctgag
     9121 gcgaactctt tacctacttt cagtgggtta acggtttcga agaaacggaa gccccaggtt
     9181 agcagttttt tactttcggc ttcacggcct ttaaaagtac gtccacccat caccgcagaa
     9241 atcaagcgca tctggccttc agtcgcagaa gcaacaaggt tgtaacctgc tttgtcagtg
     9301 tgtccggttt tgatgccgtc gacattcagg ctgttatccc ataacaggcc gttacggttc
     9361 agctggcgaa taccgttaaa cgtaaattct ttttctttat agatcgagta ttcattcggt
     9421 acgtcacgga tcaatgcctg gccgatcagc gccatatctc gcgcggagct gtactgacca
     9481 tcagcatcca gaccatgtac cgtctggaag tgggtatttt tcaggcccag tgcgttaacg
     9541 tagctgttca tcaagccaac aaaagcgtcc tggctaccag cggcaaaatc ggccatggcg
     9601 acacaagcat cgttacccga ttgcaggtta ataccgcgga tcagctgaga aaccggaacc
     9661 tgcatgcccg gtttgaggaa catcagcgaa gaacctttaa acaccgggtt accggtggcc
     9721 catgcgtcgt tgccgatagt gactaaatca gtttctttaa atttaccggc tttcattgcc
     9781 tggccgataa cgtaactggt catcattttg gtcaggctgg caggatcgcg gcggacatct
     9841 gcgttctgtt cggcgagcac tttgccggag ttatagtcaa tcaggatgta ggactccgca
     9901 tcgatctgcg gtacacccgg gatcatagtt ttgatattca ggtcatcggc atgtgcagca
     9961 gagataaagg ctgtgcaaag agccgtggtg agcgccaggc gcttcatgat acgagcggaa
    10021 aaaatggtat tcatggtctg aactacgaca tccgtgatgg aattaaaaaa gtgccctact
    10081 atagcaaatg cactaccggc aggcatctga ctttccgcgt gactttgtta acgtcattta
    10141 cagaaattga cacatcagat gcctgcttta cgctactgcg cggtagtaat aaatgactgt
    10201 aattgggctt cggtttgcaa acgttgctgc aaggtactgg cttcggcttt gctggcgaat
    10261 gggccaagct ggatccgcca gaccgcgcca ttttgagtta cgcgaccggg gacgccgaac
    10321 ttctgtccca gttgctgttg gtactgttgc gcacgagcct gatcgcttac ggccccgact
    10381 tgcaccataa agttgccgct ggcgctttgc gaggcggctt gcggtgtcac cattgcaggc
    10441 gaggttgcag gcgttgtcga cgaagctgta acaacgggct gtggcgcagg cgtcggttcg
    10501 ctgccttcca gtacaccagg cgctaaggtc gtttgagcgc cgaggaaacc gctactggtt
    10561 accggcgcgc cggtcggatc ttcgcttttt agcgtcgaat tactgaccgg aagaatgtca
    10621 ccctgcgggc cagacactga acttgttccc gcgccaccgc ttaaatcggg aggtgcaggc
    10681 agggcataag tctgtttggc gactgtggta caagccatac caggaccaga aagcgaacca
    10741 tcctgggcga caataatcgg atcgatacga actttggtgt tgtttgacgt gttaagacgg
    10801 tcagctgccg cgcgagaaag cgaaataacg cggtcgttgc cgtaagggcc gcgatcatta
    10861 atgcgcacca cgatcattcg cccgttagcc aggttagtga ttctggcgta gctggggatc
    10921 ggaagcgttg gatgggccgc cgtcagcttt gtcggatcga aagcttcgcc agaggccgtc
    10981 aggttactgc ctggttcggc atcatagatt gccgccagtc ccgcctggct aaatcgagac
    11041 ggatcctgca cgattttgta gcttttaccg tcgcgctggt aatcctgatt tgccgtcgcg
    11101 ttcagtggtt cgaaacgcgg gtccgccccg ctaatttcaa ctatggggcc gttacatacc
    11161 gcaggctgcg gtacacttac cgtctgttgc tgaccatcat cgcttgtaca tgccgcgagc
    11221 attcctgccg cgatgcagat cccgagccac tgcttacgca ttgcgcacct cttacacgct
    11281 tttcgacaac attttcctgt gggtgtggat tgacattaca atcccgaacc cagccatcag
    11341 cacaattagc gccgatcctc cataactgac cagtgggagc ggaaccccta caaccggcag
    11401 aataccgctt accataccaa tatttacgaa gacataaacg aataatatca gcattaagcc
    11461 accagccatg acgcgaccaa aggtggtttg cgctctggcg gctatccaca gcccgcgcat
    11521 gatcagcaga atgtagagag cgagcagaat cagaatgccc actaatccca gctcttccgc
    11581 cagtaccgcg aagataaagt cagtatggcg ttcggggaga aattcaagct gtgactgagt
    11641 gccgtgcagc cagcctttgc cgcgtaatcc gccggagcca atagcaattt tagactgaat
    11701 aatgtgatag cccgcgccga gtgggtctga ttccgggtcc aggagcatca ttacgcgctg
    11761 gcgctggtaa tcatgcatca ggaagaacca cagaatcgga atgaacgccg ctaccagcac
    11821 tactgcgacg ccaatcagac gccagctaag gccagagagg aacagtacaa acagaccgga
    11881 aagcgcaacg aggattgatg ttcccaggtc aggctgtgca gccaccagca gcgtgggcat
    11941 aaatatcagc accagcgcga tgccagtgtt cttcaacgat ggcgggcaaa cgtcgcggtt
    12001 gataaagcgc gcaaccatca gtggtacggc tattttggca atttccgacg gctgaaaacg
    12061 aacaataccg aggtccagcc agcgttgagc gcctttagag atggctccga aagcatctac
    12121 cgccaccagc aaaataatac agatgatata gagatagggg gcccagcctt cataaacgcg
    12181 tggaggaatt tgcgccatca ccaccatgat gaccagaccc atcgcgattt ggccgatttt
    12241 acgctccatc atgccaatat cctgaccgct ggcgctccag ataaccaggg cgctgtaaac
    12301 cagcaatgcc agtaaaatca gcagcattgt gggatcgaga tggactttat cccagaatgt
    12361 ttttttattc ggattatccg tcatgattaa tggtcctccg ctgcggcaac cgctggattt
    12421 tccgcaggca gatcggtgtt gttatcaccc agcataatgt ggtcgaggat ctggcgcatc
    12481 agcgtaccaa ccgccggacc cgcgccgccg ttctccagaa tcatggcgac agccacttgc
    12541 ggattgttgt atggcgcaaa ggcggtcatc agtttgtggt cacgtaaacg ctcggcaatt
    12601 ttgtgcgcat tataggtttc gttcgctttc agaccgaaga cctgagcggt accagatttc
    12661 gccgcaattt tgtacggtgc gctggcaaag tatttatgcg ccgtaccatt agggcggtta
    12721 gcaacaccgt acataccgtc tttcgccagc tcccagtaac cggaatgaat atcgccgacg
    12781 ggcggttcat gcggttgtac ccatggcacc tgtttgccgt cttcggcggt gctcatcagc
    12841 aaatgcggaa ccttcacgat accgtcatta atcaggatca tcagtgcctt actcatctgg
    12901 attggggtcg ctgtccagta accctggccg ataccaaccg gaatggtgtc accctgatac
    12961 cacggttttt taaagcgttt ctgtttccat tcgcgggtag gcatgttgcc ggaacgttct
    13021 tccgccaggt cgataccggt gtaatgacca taaccgaatt tacccatcca ttcggagagg
    13081 cgatcgatcc ccatatcgta ggccacctga tagaagaagg tatccgcaga ttcttccagc
    13141 gatcttgtga cattcagacg cccgtggccc cattttttcc agtcacgata acgtttttcc
    13201 gaccctggta gttgccacca gcctgggtca aacagcgtcg tattgcgcgt gatcaccccg
    13261 gcgcttaatg ccgaaaccgc cacatagggt ttaactgtag acgcgggagg ataaaccccc
    13321 tgtgtggcgc ggttcaccag cggtgtattc gggtcgttca gcaaggcgga ataatctttg
    13381 ctggagatac cgtcaacaaa caggtttggg tcataactag gcgtggaaac cagcgccagc
    13441 accccacctg tacgcggatc ggtaacaacc acagctgcgc ggctacccgc cagcagcgtt
    13501 tcaatatatt gctggagttt gagatccagc gtcaggtaaa tatcgtgtcc ggcttgcggt
    13561 ggtacttctt ttaactggcg gataacacgc ccacggttgt taacttcaac ctcttcataa
    13621 ccggtctgac cgtgcagcac gtcttcatag taacgctcaa tgcccagctt accaatatca
    13681 tgcgttgccg catagttggc cagtttgccg tcattattca ggcgttcgac gtctttatcg
    13741 ttgattttcg acacatatcc gatgacgtgg gtcaacgccg aaccgtaagg atagtaacga
    13801 cgtttatagc ctttaacttc gacacccgga aaacggtact gattgacggc aaagcgagct
    13861 acttgtactt cggtcaggtt agttttcacc ggaatagagg tgaaacggtg tgaacgtgcg
    13921 cgctcttttc ggaatgcagc aatatcgtca tcggtcagat ctaccacgct gcgcaaagcg
    13981 tccagcgttt gctgcacgtt atcgactttc tccggcatca tttctatctg gtagatagtg
    14041 cggttgaggg ccagagggat accgttacgg tcgtagataa tgccgcggct gggcgcgata
    14101 ggcaccagct taatgcggtt ttcattagag cgggtctggt agtcggtaaa gcgaacaatt
    14161 tgcagattat acaggttggc gataagcacg ccggtcagca gcaaaatccc caaaaaggcg
    14221 accagcgccc ggcgcacaaa cagcgcggac tctgccgtat agtcgcgaaa agagttctgt
    14281 agtttcatcc gctgcgtttt ctactcaaag ctcccttatc actcacggtg ataaggatgg
    14341 ttggtggtga tgctccacgc ccggtacaga ctctctgcga ccagcacgcg aaccagcgga
    14401 tgggggaggg taagcgccga cagcgaccag ctctgctcag ccgccgcttt acaggcaggc
    14461 gacaaccctt caggcccgcc aatcagtaga cttacgtcgc gaccatccag cttccagcgt
    14521 tccagctcag cggctaactg cggcgtatcc cagggcttgc ctggaatatc gaggatgaca
    14581 atgcggtttt tgcctgcggc cgccaacatc tgctcaccct ctttgtcgag tatgcgcttg
    14641 atgtccgcat tcttgccgcg tttgccggcc ggaatttcaa tcagctcgaa gggcatatct
    14701 ttcggaaaac gacgcaggta ctcggtaaaa ccggtttgta cccagtccgg cattttcgtt
    14761 cccacggcga caagttgcag cttcacgcat taactccaga gtttttccag ttcatacagg
    14821 cgacggctct cttcctgcat gacatggaca atcacatcgc ccaaatccac gacaatccag
    14881 tcggcgctgt tttcaccttc tacgccgagc ggtaacaggc ccgctgcgcg agactcctgc
    14941 acaacgtggt cagcaatgga cataacatga cggctggacg tacccgtaca gatgatcatg
    15001 cagtcggtga tgctggattt gccttgaacg tctaaggcga tgatgtcctg acctttgagg
    15061 tcatcaattt tgtcgataac aaaatcctgg agtgctttac cctgcaagtt ttccccctgg
    15121 gtgaatcaaa tagataaaaa tggtctgtca gtatacctga accagaggcg atttcgggac
    15181 aattgtcgcc gaatcggctt tcgaaagtgg gctatcatcc caccccgcgc cgcagattgc
    15241 atcgccattt ttgtaaaaca atttctacaa agtcgtgtct ggcggaaaaa gtctggctgc
    15301 ggagaatatc agcctgcccg gggctgtcaa tggtcgttgc ggcttacccg taaaaaaaca
    15361 gaaaagtcat gcattctcga ccccgatggc acggctattg aggacgcgta gcgtcgcgaa
    15421 tttttggttg atatcaatgg cgctccaaca cccctggtca acgcgaaaat gccacataga
    15481 ctcggcaggc atgccaatta aacgagcaat taacagactc agtacgccct gatggctgac
    15541 gattaaaata ttctgatagt gctgatattc actaagcctt gcgataaagc gttccacacg
    15601 ttgcgaaaat gcctgaaatc cttcaccgtt cgtggggata gcatgctgcc agtcattgca
    15661 ccacgcgcta tagttttcgg catcttcttg catgaggtcg cgatgatgtc gcatctccca
    15721 gtcgccaaaa aacatttcgt tgagttcagg gatgatatga acggggagct ggcggtcact
    15781 gagaaccagt cgcgcggtat gctgtgcccg ttccagttca ctgcataaaa ccaggtcaaa
    15841 ggaaacatca tctagcagtg tatgcagatt ttgcgcttgc tcaataccgc gtgcggtcag
    15901 gggtgtgggc gcatgaccgc tgtacagacc atcgacattc gcttgtgttt cgccatgacg
    15961 aattaaccac agtcgcatca tgccctccgt aacgacaggt atcagcgata caagccttgt
    16021 tggttaatgt aagtcaatac cggttccggc aataaatcct cacacgattc accgttttgc
    16081 aaacgttcgc ggatgatggt cgccgagatg ttaaaccacg gcgtttcagc cagataaatt
    16141 ttaccggcag gctgaaggtg aagatcttcc gggttatgtg tcaaatgatc ttccagccat
    16201 tgctggtatt gcggttgcgc catttcaagt gggtaacctg gacgccgaca gacgatcaaa
    16261 tgtgcattgt cgagtatcgt ttcgtattcg taccaggtcg gaaaggtcag caatgaatcc
    16321 tgaccaataa taaacgccag cggcacgtcc ggtccttgtt cctgccgcca ctctttcagt
    16381 gtttgtgcag tgtaagaggg ggcattgcgc tttagctcgc gttcatcaag agtaaataat
    16441 ggcttgtcgg caatcgccag ttcaagcatg tgtttacgct gcacgctgtt cgcttccggc
    16501 tggggacgat gcggaggaac attattaggg atgattgtga cccgcgtcag accaatcaaa
    16561 ttcgccagcg tttccacggg ttttagatga ccatagtgca ccggatcaaa ggtgccgcca
    16621 aacagagcct gtaaagattt catatcaacc gtcgataaat acgtccgcca ggggtttatg
    16681 gcacaacaga agagataacc cttccagctc tgcccacact gactgaccgt aatcttgttt
    16741 gagggtgagt tccgttcgtg tcaggagttg cacggcctga cgtaactgcg gctgacttaa
    16801 gcgatttaac gcctcgccca tcatgccccg gcggttctgc catacccgat gcttatcaaa
    16861 caacgcacgc agtggcgtat gggcagactg gcgtttcagg ttaaccagta acaacagttc
    16921 acgttgtaat gtgcgcaaca aaataaccgg ctcgctgcct tccagacgca gttgctgaag
    16981 aatatgcaac gcgcgcttac tttttcccat caacaaagca tcaacccaat gaaaaggggt
    17041 gaaatgcgcg gcatcattca ccgcctgttc aacgcgcggt aatgtcagtt tgccgtctgg
    17101 ccagagcagc gataaacgct ccagtgcctg agccagcgcc agcaggttac cttcataaca
    17161 gtagcagagc acctgattcg ccgcgtcatc cagttctaag ttgagctgtt ttgcgcgcac
    17221 agcaacccag cggggaagct gagcctgctc cggtgtctga caggtcacct gcacgctgcg
    17281 attcgcaaga gcagtaaacc aggcggcatt ttcttgcgct ttgcttaatt tattaccgcg
    17341 gacgatcaac agcaggtcgt catgcagaag tccggtgagt gtgagaagtt gttcattgat
    17401 cgccgcattc ggtccgtttt ctggtaacaa cagcaatagc gtttgtcgac tggcaaacag
    17461 actcatagcc tggcataacg aaaagatcgc attccagtca gtgttgggat caatggaaaa
    17521 agtgtggtgt tcttcgaatc cttgtgccgc agctacctgg cgaacagcgt cctggctttc
    17581 ctgcaataac agaggatcgt taccaagtaa aagatacgcc gcgcgcagcc cttcattgag
    17641 ctgcgcgcgg agttgttccg ggtacaaccg aatcatcagt tacccagcat ggtggagacg
    17701 cgcgcaggcg ttgccggagt atccgttgtg gtcgacgtct gttcttcgtc ggaacgaata
    17761 tccgcagcac ggatgcttgg cagcttacga atcagctgtt cggcagcacg gtcgtacatc
    17821 tcttttacga tcatgtcttg ttcgttatct ttcgctaacg ccatttgcgg gttatcgaag
    17881 aatgaacgga agactttggc gctaattggg tagatatcac ggccggggat caacacggtc
    17941 gcattcaccg tcatgatcat ctgatactct gctgtttgac cgttacggaa taccgatgcg
    18001 gtatctttcg cgatgctcac tttacccaaa cgcagggatg gaacgtcctt acgcgtggtt
    18061 tctttatcaa gcaactcgac accattcaga cgtaactggt tacgcaccgc acggcttaat
    18121 ggcccgttcg gatcgcctga gtccaggatc atgaccttca tagtggaagg aacctgcgtg
    18181 gtgtcacgca gatgccagcc acacccggcg gtgattaaca ccgccagaga taacaacaat
    18241 gttgccagat atcgcacgct tcctcccgcg cttagccaac gaccagattg aggagtttac
    18301 ctggtacgta aatcacttta cgtacagtaa cgccatcaag atattttgct accagatgtt
    18361 cctggccagc acgttcgcga acctgttctt ccgttgcgtc caccggaacg gtgattttgg
    18421 cacggacttt accgttaacc tgcaccacga ccagcgtgga gtcttccacc atcgcttttt
    18481 cgtcagcaac cggccacggc gcgttgtcga tatcgccttc gcctttgagt tcctgccaca
    18541 gcgtgaagca gatgtgcggg gtgaacgggt tgagcatacg gacaacggcc agcagtgctt
    18601 cctgcatcag agcgcgatcc tgctcgccat cggtcggtgc tttcgccagt ttgttcatca
    18661 gctccataat cgccgcaatt gcggtgttga aggtctgacg acggccgata tcatcggtca
    18721 ctttagcgat agttttatgc acatcgcgac gcagcgcttt ctgatcttca gtcagcgcat
    18781 caacgttcag tgccgcaaca tcaccttttg ctgtgtgctc gtacaccagt ttccagacac
    18841 gtttcaggaa gcggttagcc ccttccacac cggattcctg ccattcgaga gtcatatcag
    18901 ccggagaagc aaacatcata aacagacgaa cggtgtccgc gccgtaacgt tcaaccatca
    18961 cctgcgggtc gataccgttg ttcttcgact tggacatttt gctcatgccg gtataaacca
    19021 gttcatggcc tgccgcatct ttcgctttca cgatacggcc tttctcgtca cgttcaacga
    19081 tagcatcaac cggggaaacc cagttacgtt cgccgttttc gccaacatag tagaaggcat
    19141 ctgccagcac cataccctga cacagcaact gtttcgctgg ttcgtcagag ttcaccatgc
    19201 ctgcatcacg catcagtttg tggaagaagc ggaagtagag caggtgcata atggcgtgtt
    19261 cgataccacc aatgtagata tccaccggca gccagtagtt agccgcttcg gaatccagca
    19321 taccttcttt gtactctggg caagtgtagc gcgcatagta ccaggaggac tccataaagg
    19381 tgtcgaaagt gtcggtttca cgcagcgctg gcataccgtt aacggtagtt tttgcccact
    19441 ccggatctgc tttaatcggg ctggtaatgc cgtccatcac cacatcttcc ggcaggatca
    19501 ccggcagctg gtcgtccggg gttggcatta cggtaccgtc ttccagcgtc accatcggaa
    19561 tcggcgcgcc ccagtaacgc tgacgagaaa caccccagtc gcgcaggcgg tagttcactt
    19621 tacgctcgcc aacgcccatc gcagtcagtt tatcggcgat ggcgttgaag gccgcttcat
    19681 ggtcaagacc gttgaactcg ccagagttga acagcacgcc tttttcagtc agggcttgct
    19741 gagaaagatc tggctcagag ccgtcagccg ccaggataac cggtttgatg ttcaggccgt
    19801 atttagaggc aaactcgtag tcgcgctggt cgtgacccgg aaccgccata actgcgcccg
    19861 tgccgtactc catcaatacg aagtttgctg cccaaacggg aatttcttcg cccgttaatg
    19921 ggtgaacagc tttaaagcca gtatcgacgc cttttttctc cagcgtcgcc atttcagctt
    19981 cggcaacttt ggtgttacgg cattcgtcaa taaaggccgc cagttcagga ttattttccg
    20041 ccgctttctg cgccagcgga tgacccgcag ctaccgccag gtaggtacaa cccataaagg
    20101 tgtccgggcg ggtagtgtaa acggtcagcg tgttgtcata gtcgttaacg ttgaaggtga
    20161 tctccacgcc ttcggaacga ccgatccagt tacgctgcat ggttttaacg gtgtctggcc
    20221 agtgatccag tttatccaga tcgttgagca gctcgtcagc gtaagcagtg attttgataa
    20281 accactgcgg gatctcttta cgttcaactt tggtatcgca gcgccagcag cagccgtcga
    20341 taacttgttc gttcgccagt acggtctggt cgttcgggca ccagttgacc gcagaagtct
    20401 ttttatatac caggcctttt ttatacagct cggtgaagaa tttctgttcc caacggtagt
    20461 attccggcgt acaggttgcc agctcgcggc tccagtcata accaaagccc agcattttga
    20521 gctggttttt catatacgcg atgttgtcgt acgtccacgg tgccggagcg gtgttgtttt
    20581 tcaccgccgc gccttccgca ggcagaccaa acgcgtccca gccgatcggc tgcagaacgt
    20641 ttttgcccag catgcgctgg taacgggcga tcacgtcacc gatggtgtag ttacgtacgt
    20701 ggcccatgtg tagtcgacca gaaggatagg gaagcataga caggcagtaa tacttctctt
    20761 tgctctcgtc ttcggttact tcaaatgtgc gcttctcatc ccaatgaagc tgtactttgg
    20821 attctatctc ttccgggcgg tattgctctt gcatggcagc cagtggtcct gttttcaata
    20881 cggctacaaa tgtagcgttg aggtggtttt tcagatccgc atagcatagc ccaaacgtcc
    20941 gcgtcaaaac agcctttcgc gcactcgacg ttgaaatgat gcccggatta ttcgtacatt
    21001 aatttacaga gtttgtgggc gtattagcaa agcaaggaac aaagaacgtc tattattata
    21061 gtcagttaac gacccgggag atgaaacgat gaacaaggtt gctcaatatt accgtgaact
    21121 ggtcgcgtca ctgaacgaac gcctgcgcaa tggcgaacgt gatatcgacg cactggtgga
    21181 acaggcgcgc gagcgcgtaa taaaaacagg ggagttaacg cgaaccgagc tcgatgagct
    21241 gacgcgagct gtcagacgtg acctggaaga gttcgccatg agctatgaag agagcctgaa
    21301 agaagaatct gacagcgtct ttatgcgggt gattaaagaa agcttgtggc aggagctggc
    21361 agacatcacc gataaaacgc agcttgaatg gcgcgaagtt ttccaggacc tcaatcatca
    21421 tggggtttat cacagcggag aagtggtcgg gctgggaaat ctggtctgcg agaaatgtca
    21481 cttccatctc ccgatctaca caccggaagt gctgacgcta tgcccgaaat gtggttatga
    21541 ccagttccag agacgcccgt ttgagccgta atagtctgat ttcgcatagc aataaagaga
    21601 atatgggcag aatgaaattc cccggccctt gtttttattt ctacagtaaa gcttcagtct
    21661 cacgaatttc ctgactgaca tctctggttc cggatgactg aactttacga tacaggtcga
    21721 gagcctgtcg gagatcgaca ggtcttcctt ctccgtagtg atacataatg gccaggttat
    21781 accaggcagt ggcgtcattg cattcagcgg ctttcgtaaa ccattcaaag gcagcctgat
    21841 aatctttctc aacgccttgt ccgtgtttat acatataacc gatgttgacg taagcgccga
    21901 cgctttcctg agcggcagcc ttcagatacc agtacatcgc ttgcttatag tcctggtcca
    21961 ccccttcgcc agcgttataa tcccatgcaa tttgaaactg ggcgtgccga ttaccctgct
    22021 gtgcgctttt caaataccag aatgcggcga gtgtcttgtt ttgagcaacg ccttctccgt
    22081 ctttataaag atcggccaga ttgtcttgtg cgtaactatg gccttgtaat gcagcttgtt
    22141 tgtaccagaa aaatgccagc gtatagtctt gtgcgactcc gttgccattt ctgtacatcc
    22201 atccaagatt attttgcgca taagacattc cgctctccgc ggctatctga taccaggtaa
    22261 ccgcttcttt atagttgggg tcttctccac gatccagtat ccagccaagg gcgttggaag
    22321 cgaaagtatg gccttgtagg gcagcttttt tcagccagaa gatggcttgc tcattatctt
    22381 tatgacggct tttatcctcg gtatactttt gccctaagga atactgtgct tcacaatgac
    22441 cttgctcagc ggccagcttc agccagtaaa aggccttttc gtcgtccgga gagtcaattg
    22501 cgctatcgcg attataataa aacccaacaa tatattgagc ctcgcaatcg cctttttcag
    22561 cacgttcgat aatctcatct attgataaat tatcgcagca acttgacgta aaaattatta
    22621 tcatttcctt atggttgtag ataaaatata ttatattttt atgtgttcca tttttgcgta
    22681 agccttattt tatgcgtatt atgttgcgtc agaaaagtat aaataactga gtctcgaaat
    22741 gtagtttgca ttcattaaat gaatgtttaa ggataaatta tatggacatg gggtcgcaaa
    22801 aaatattgtt tgcactttcc actccgatgg aaatacgcaa tgaatgctgt ttaccttcgc
    22861 attcatcccc caaaatgtat ttaggtacat gcttttttga cctttcttcc tcatggggaa
    22921 ttgatgaccg tgatgacctg ctgcgcacaa ttcaccgaat gatcgagaac ggtcatgccg
    22981 ctcggttggc agggttttat caccgctggt ttcgttattc tccatgtgaa tggcgtgact
    23041 atcttgctga attaaatgag caaggtcagg cttatgcgca atttgtcgcc agcacagccg
    23101 aatgctgtgg tgagggagga attaaagcat gggattatgt ccggatgggt tttttaagtc
    23161 gaatgggcgt acttaataac tggttgagtg aagaagaaag tctatggatc ctgtcgcgta
    23221 ttcatctacg ggctctacgt tattatagta actggcggca atactttgcc ggttatacct
    23281 ttggtcggca atattggcag tctcccgaag atgatcatct gcctttatta cgcgaattct
    23341 tagcgcgtaa agaatacgac gattccggca atgatatgtt ttatcaatta tttgccagtg
    23401 atgatgcgta ttaccctacc ttgtcctggc aaccactggc tgactacccc acatgcccgg
    23461 aaacgcttaa ggatatgagc gacttatgaa aaattgctgg aagatcctcg atatagagga
    23521 aacgactgac gtcgatatta tccgccgcgc ttatctggcg ctgttaccgt cctttcatcc
    23581 agaaaccgat ccgcagggtt ttaaacaact tcgtcaggcg tatgaggagg cgctacggat
    23641 tgcgcagtcg cctgctaaat ctgtttggca accagaagaa tatgaggtag cagaacatga
    23701 aattctgctc gcctttcgcg cgttacttgc ctctgatagc gaacgttttc tgccctccgc
    23761 ctggcagcga ttcattcagc aattaaatta ttgctcgatg gaagagattg atgaattacg
    23821 ctggtcgctg tgcacaatcg ccatgaacac tgcccattta tccttcgagt gcgtggtgtt
    23881 attagcagaa agattgcggt ggttgcagga ggaaaacgtc ggggaaatag acgaagaaga
    23941 actggaatcc tttttatatg ccattgcgaa ggggaatgtt tttaacttcc agaccattct
    24001 gcatctgccc gttgccgtgc aaaatgacac catcgatttt taccaaatgt tcgctcggat
    24061 ttggtcatcg catccagaat ggctgacatt gtatttagcg caacatcgcg cagtgattat
    24121 ccccgatgat gcaaaactgc acagaaattt actccgctgg tatagcgcaa gccgcctggg
    24181 aatccccgaa ctcctggatt acgcccggtc gtggcgggaa gctgaacctg ataatgaaga
    24241 tgcgcgttat tatgaatacg cacaacgcgt ctattgtgga gaaggcgaaa gcctgctggc
    24301 agaactttgt tactactggc gcgagtatcc ctccacccag gcggatgctt taatgttgca
    24361 atggtgccgt cagcatcggg tcgattatta cccattagtg gtgatgatga ttgaagcgcg
    24421 tgatctggtt aacgatcagg gaaaaccgct actttatgtc cccggcgaca gcgcccgtac
    24481 gcgttttcat ttatacgaaa tactcagcga tgaaaaactc tctgcgctgg ggcgttcact
    24541 ggtcgagatg gttttgcaca aaggacgtaa gccgcggatc tcactcacgc gtgatacaga
    24601 acatccctta tggccattat atttagttgc caaacaatta gtgcaggcca gccaaccgac
    24661 agaagaatca ttaatgccga ttgtgagccg ccttgatgca gaagatcgtt gtccactgga
    24721 agcattaatt attcgtcgat tattaattca ggcggcgaat tttaccggtc aggaaaccgt
    24781 cgagccggag ccgcaaccgc agccaatgcc cgttgacgat ggcgggctgg gctgtctggg
    24841 cgtcatcaaa attattttct acatttttat ctttgccggt ttgataggga aaatactcca
    24901 tctcttcggg tgacagtcga cgttaatgtg cgggaagtcc ggcaagtaat ctttccgcat
    24961 ttttgtaatg ttgtttggcg gccagtttta accatttttg agattcataa tggttaacat
    25021 taataccagt gccatttctg tagatcaaac ccaaagtata ttgagcatga gcataccctt
    25081 gttcagctgc ttccttatac cagagaattg ctttacgggt atcgggtttg actcccaggc
    25141 ctttcaggta gatccacccc agtttgcttt gtgcgcgcgg attttcctgt tctgctgatt
    25201 gctcatacca gtatctcgcc tgtgtataat ccagtggttt accgtcgcta ccatgaagat
    25261 aaaattcgcc aaggcgatat tgcgatgttg agtgaccatg aatcgcggaa agctccgtgg
    25321 ctgccgtggt taacggtaga ctgcctggtg atcttaaata atcaaaggca taattcacca
    25381 tggctttatc acgcaacgtc gttttcttgg gttggctaat acaggtaatt gctgcaacta
    25441 taaa
//
LOCUS       DADLZM010000047        25371 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLZM010000047 DADLZM010000000
VERSION     DADLZM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.047"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            137..952
                     /gene="ygiD"
                     /locus_tag="JNY73_001894"
     CDS             137..952
                     /gene="ygiD"
                     /locus_tag="JNY73_001894"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY8047923.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(990..2150)
                     /locus_tag="JNY73_001895"
     CDS             complement(990..2150)
                     /locus_tag="JNY73_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY8047924.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(2156..2827)
                     /locus_tag="JNY73_001896"
     CDS             complement(2156..2827)
                     /locus_tag="JNY73_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY8047925.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2975..4456)
                     /gene="tolC"
                     /locus_tag="JNY73_001897"
     CDS             complement(2975..4456)
                     /gene="tolC"
                     /locus_tag="JNY73_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY8047926.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            4661..5290
                     /gene="nudF"
                     /locus_tag="JNY73_001898"
     CDS             4661..5290
                     /gene="nudF"
                     /locus_tag="JNY73_001898"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY8047927.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5291..5713
                     /locus_tag="JNY73_001899"
     CDS             5291..5713
                     /locus_tag="JNY73_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY8047928.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            5738..6565
                     /gene="cpdA"
                     /locus_tag="JNY73_001900"
     CDS             5738..6565
                     /gene="cpdA"
                     /locus_tag="JNY73_001900"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY8047929.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            6565..7146
                     /gene="yqiA"
                     /locus_tag="JNY73_001901"
     CDS             6565..7146
                     /gene="yqiA"
                     /locus_tag="JNY73_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY8047930.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            7175..9067
                     /gene="parE"
                     /locus_tag="JNY73_001902"
     CDS             7175..9067
                     /gene="parE"
                     /locus_tag="JNY73_001902"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY8047931.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(9115..9429)
                     /locus_tag="JNY73_001903"
     CDS             complement(9115..9429)
                     /locus_tag="JNY73_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8047932.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(9460..10041)
                     /gene="mdaB"
                     /locus_tag="JNY73_001904"
     CDS             complement(9460..10041)
                     /gene="mdaB"
                     /locus_tag="JNY73_001904"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY8047933.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            10360..10692
                     /locus_tag="JNY73_001905"
     CDS             10360..10692
                     /locus_tag="JNY73_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY8047934.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(10738..12087)
                     /gene="qseC"
                     /locus_tag="JNY73_001906"
     CDS             complement(10738..12087)
                     /gene="qseC"
                     /locus_tag="JNY73_001906"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY8047935.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTPDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLIGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLLALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(12084..12743)
                     /gene="qseB"
                     /locus_tag="JNY73_001907"
     CDS             complement(12084..12743)
                     /gene="qseB"
                     /locus_tag="JNY73_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY8047936.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLSSDFIRTVHGIGYTLGE
                     K"
     gene            12895..13287
                     /gene="ygiW"
                     /locus_tag="JNY73_001908"
     CDS             12895..13287
                     /gene="ygiW"
                     /locus_tag="JNY73_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY8047937.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            13340..13822
                     /locus_tag="JNY73_001909"
     CDS             13340..13822
                     /locus_tag="JNY73_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8047938.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            14368..16626
                     /gene="parC"
                     /locus_tag="JNY73_001910"
     CDS             14368..16626
                     /gene="parC"
                     /locus_tag="JNY73_001910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY8047939.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            16860..17597
                     /gene="plsC"
                     /locus_tag="JNY73_001911"
                     /gene_synonym="parF"
     CDS             16860..17597
                     /gene="plsC"
                     /locus_tag="JNY73_001911"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY8047940.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            17672..19084
                     /gene="ftsP"
                     /locus_tag="JNY73_001912"
     CDS             17672..19084
                     /gene="ftsP"
                     /locus_tag="JNY73_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY8047941.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            19195..21414
                     /locus_tag="JNY73_001913"
     CDS             19195..21414
                     /locus_tag="JNY73_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY8047942.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSLINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21457..21714)
                     /gene="yqhH"
                     /locus_tag="JNY73_001914"
     CDS             complement(21457..21714)
                     /gene="yqhH"
                     /locus_tag="JNY73_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY8047943.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(21765..22691)
                     /locus_tag="JNY73_001915"
     CDS             complement(21765..22691)
                     /locus_tag="JNY73_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY8047944.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(22891..23718)
                     /gene="dkgA"
                     /locus_tag="JNY73_001916"
     CDS             complement(22891..23718)
                     /gene="dkgA"
                     /locus_tag="JNY73_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY8047945.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDEPGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(23823..24986)
                     /gene="yqhD"
                     /locus_tag="JNY73_001917"
     CDS             complement(23823..24986)
                     /gene="yqhD"
                     /locus_tag="JNY73_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY8047946.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKVAR"
     gene            25180..>25371
                     /locus_tag="JNY73_001918"
     CDS             25180..>25371
                     /locus_tag="JNY73_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8047947.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         5896 a         6327 c         6779 g         6369 t
ORIGIN      
        1 cagaatgaga ggtactgaca tcgacaactc cttataaatc ttcaccttca ggcagatcgt
       61 caaaataact gatgatcatc atcattgtta tccgggttct tactccgccg aacaagcgta
      121 ttgtgaggat atcaaaatga cacctttagt taaggatatc atcatgtctt caacacgtat
      181 gccagcattg tttttaggtc acggtagtcc gatgaacgtg ctggaagata atttgtatac
      241 ccgcagctgg cagaagttgg ggatgacatt gccacgcccg caagcgattg tggtggtttc
      301 ggctcactgg tttacccgtg gaacaggagt gaccgcgatg gagacgccgc ccacgattca
      361 tgactttggt ggcttcccgc aggcgctgta cgatacgcat tatcctgctc cgggttcgcc
      421 tgcgctggca cagcgtctgg ttgagctgtt agcgccgatc ccggtgacgc tggataaaga
      481 agcctggggc tttgaccacg gctcgtgggg cgtgctgatt aagatgtatc ctgacgccga
      541 tatccctatg gtgcagttga gtatcgacag tagcaaacct gccgcctggc atttcgaaat
      601 ggggcgcaaa ctcgcggcgt tgcgtgatga aggaataatg ttggtcgcca gcggtaacgt
      661 ggtgcataac ctgcgcacag tgaagtggca cggtgatagt tcaccgtatc cgtgggcgac
      721 gtcgtttaat gagtatgtga aagcgaatct gacgtggcaa gggccggtag agcagcatcc
      781 tctggtgaat tatctcgacc atgaaggtgg cgcgttatcg aacccaacgc cagagcacta
      841 tctgccgttg ttgtatgtgt taggtgtgtg ggatgggcag gagccaatta ccattccggt
      901 cgatggtata gaaatgggca gtttaagtat gctgtcggtg cagataggct aacaattacc
      961 ggatggcgag tgccatccgg tatcgtggct tattcaacaa aaatatgtgg ataaaaccga
     1021 gacatatcct gggtgatcaa cgcacggtct tcacgaatgc caattccggc aggttgatcg
     1081 ttcaccagcc agctaccaat cagcatatag ctgtcgccga atttcggtaa cgggtggaat
     1141 tgctgaacaa tcatcccttc ttcgccatac ggaccttccg ctgcttcaat ggttttgccg
     1201 ttctcaatga tcgacacgtt tgcgccttca cgggagaaga tcggtttaac cacatatttt
     1261 tccatttgcg gatgatcatc ttccgcaaaa taagcgggca gcaggttcgg gtgattcggg
     1321 aacatctccc acagtagcgg tagaagtgcc ttgttggaga taatactctt ccacgccggt
     1381 tccagccagc gtacgccagc atcttccagc ttggtggaga acatttcgcg cagcataaat
     1441 tcccacggat aaagcttgaa caggttagaa atcacctgat cctgtaaatc cgtgaactgg
     1501 cctttttcgc ctaaacctat ctcgtcgatg tagaggaact cggtggcgat ttcagcttcg
     1561 gttgcgcagt cctgcaaata ctgaatggtt ccgcgatctt ccaccgtgtc gcgacagcag
     1621 gtgagatgca gcaactggaa gccacactgt tcacgcagct caacgaagcg atcgatcagt
     1681 ttttcttgca gactgttaaa ctggtcgctg ccctccggca agttaccggc gttaagctga
     1741 tcttccagcc agatccactg aaagaacgcc gcctcgtata gtgacgttgg cgtatcggcg
     1801 ttattttcca gaagtttagg ttcgccagtg ccatcccacg ccagatcaag acgcgaataa
     1861 agcgatggct ggtgagtcag ccatgattga cgcacaaaac tccaggtgtg ttttggtatg
     1921 cggaatttgg tcatcagctc atcgctggcg atcacttttt ccaccacttt caggcacatc
     1981 tggtgcagtt cggcggtgac ttcttccagc ttttcaacct gggcgagggt caacttgtag
     2041 taagcatctt cacaccagta cggctcgccg tacatggtgt gaaaattgaa accgtattcg
     2101 tgggctttct cacgccagtc cgggcgctcg gtaatactga ctctttccat cggtatcagc
     2161 cacccattga acgagaagag gtgccggttg cgctacgctg catagtgctt tgtttggcaa
     2221 cagattcacc aaaaccgcca cgggtaacgg tagtggtggt cgccggtttt ggtgccattg
     2281 ccgtcttcgg tacggtcatg gtgcggcctg gctgggctgc gccatagttt ttacccgtcg
     2341 cgtcggtata tttaccgtaa gccggactgg ccgggttttt cgaggagaac agcggctgct
     2401 gtgcaaatcc cgcgccgccg cccatcagac gccccatcat gtaaccggcc atcagcggca
     2461 tccagaaact cccgctggat tgctgagcct gcgcctggtt ttctggtgcc atgccagcct
     2521 gggctggtgc ttgctgacac tgaccttcac caaattcagc aacacagtct tcacgggtgg
     2581 cgtatttcgg cgcagtacgt tcggcttctt tcagcgcatt gttgtacgcg gtggtacatt
     2641 cggcgctttt gcctgggttt gcagctgaac agtcgtcagc attttgatag agagacactg
     2701 tttcatcact cttttcacag ccagccagca taaaaacagt ggcaaccgcg agagcgactg
     2761 gtgtcagatg gcgtgcgctc cagtttttgc ggaacgatgc gtggcgtatg gattttgtcc
     2821 gtttcatttt tgtcttccgg gaccagtggt aaatacccat cagaatagag gatggctggt
     2881 cgaaattgaa gcgagaaggg ggaagaatgc ggcagataac ccgtatcttt acgttgcctt
     2941 acgttcagac ggggccgaag ccccgtcgtc gtcatcagtt acggaaaggg ttatgaccgt
     3001 tactggtggt agtgcgtgcg gatgtttgct gaacgacggg tgccgggcta tcaggcgcat
     3061 aaccatcagc aatagcattc tgttccggcg tttgcggggc aacgttttcc ggattagtgg
     3121 aaaccggttt gctcagcgca ttgttcagtg ccagcaaatc ctgctcgttc aacgtaccca
     3181 gggctgactt aatattcagc tgattaatca ggtagttata acgcgcattc gccagctctt
     3241 gcttggcgtt gtacagcgtg gtggtcgcat ccaacacatc aacaatggta cgcgtaccga
     3301 ccgagtagcc cgcttccatc gcgtctaatg agctttgcgc ggaaactacg gcttgtttgt
     3361 aggcgttaat actactgata gaagcattaa tgttgttgaa ggaggaacgt acggtttgca
     3421 cgacgctacg atgtgcgctt tccagttgct cactggcacc aacaaagttg tactgtgcct
     3481 gtttcacctg cgagttaacc attccgccct gataaatcgg cagcgagaag ctcaggccaa
     3541 ctttgttctg gcccatattg ctatcgtcat actgggtacc agcggcacca cgggttttcg
     3601 aaccgctata agaggtgtca gaaatcccgg tagaagccgt taaatccaga gtcggtaagt
     3661 gaccatcctg cgcctggcga atttgctcgc gcgccaggtc ctggctcaag cgtgcctgta
     3721 acagcgacag gttgcgtttt tcggcttctt tcagcagcgc gttaaccggc tgtggtttgt
     3781 cggttttaaa gttttcgaca ttcagcgccg ccagttccgg atagtagtta ccggtgatct
     3841 ggcgcagctg ctctaccgcg ttatcaaggt tattacgtgc ggtcacttcg ttcgccagca
     3901 cggtatcgta ctgcgcgcgg gcgttctgca cgtcggtgat cgctaccagg cccacgttaa
     3961 aacgttgggt ggtttgatct aattgacggt agatcgcttc tttttgtgcc tgtgtatagg
     4021 aaagaacgtc aatagcattc aacacgttga aataagcggt cgcggtgttg aggatcaagg
     4081 tttgctgatc ggtctgatat gtgacgtcct gaatccctgc tgctttttcc tgcagcgtta
     4141 acgcacgcca tttcgacata tcaaaaatgg attgagttaa ctgcagggac gcactggtcg
     4201 cgttcgagtt gatgccgttc gcgtcgcggt agccgttgct ataggtgtaa tctgcaccta
     4261 aacctagctg tggcagtaat ggactgcgcg cttcattaat tttttcaaag gcagcatcac
     4321 gatcggcggc agacttacgc aattccgggt tactaaggcg tgcttgctga taaacttgca
     4381 tcaggttctc ggcctggctc aacgaactga acccagaaag gctcaggccg ataagaatgg
     4441 ggagcaattt cttcatttgc attccttgtg gtgaagcagt atttagcgcg atcaaactgt
     4501 aaaattattg cggattctag cagaagccgc taccgcaaaa ggttggcgtt acgtgccata
     4561 gggcgttaat gtgcctcaat ttaacatatg actgctcaaa cggcagtcaa acgtcgctga
     4621 aattcacatt taattcacta ttagtgccag gacattaaca atgcttaagc cagacaacct
     4681 gcccgttaca tttggcaaaa acgatgtaga aattattgca cgagaaacac tttatcgcgg
     4741 ctttttttca ttagatcttt atagatttcg tcatcgtcta ttcaacgggc aaatgagtca
     4801 tgaggtacgg cgggaaattt ttgagcgcgg tcacgccgca gtcttgctac cctttgaccc
     4861 agtgcgtgat gaagttgtgc tgattgagca gattcggatt gccgcgtacg acaccagcga
     4921 aaccccctgg ctactggaga tggttgccgg gatgattgaa gagggtgaaa gtgtggaaga
     4981 tgttgcccgt cgcgaagcga ttgaagaggc gggactgata gtcaaacgga ccaaaccggt
     5041 gttaagtttc ctggcaagcc cggggggcac cagtgagcgt tcgtcaatta tggtgggcga
     5101 agtggacgcc acgaccgcaa gcggtattca tggtctggct gatgaaaacg aagatattcg
     5161 cgttcatgtg gtaagccggg aacaggcata ccagtgggta gaagagggga aaatcgacaa
     5221 cgcagcgtcg gtcatcgctt tgcaatggct gcagctgcat catcaagcgt taaaaaatga
     5281 gtgggcataa atgaagcgtt acacacctga ctttcctgaa atgatgcgcc tgtgcgagat
     5341 gaacttttca caattgcgcc gtttgttacc gcgcaatgac gcacccggcg aaactgtaag
     5401 ctatcaggtg gcaaacgcac aatatcggct gacgattgtg gaatcgaccc gatacactac
     5461 cctggtgaca atagaacaga ctgcgcccgc gatcagttac tggagccttc cgtcaatgac
     5521 ggtgcgtctg tatcatgacg cgatggtggc tgaagtgtgt tcaagccagc agatttttcg
     5581 cttcaaagcg cggtatgatt atcctaataa aaagttgcat caacgcgacg aaaagcatca
     5641 aattaaccag tttttagcgg actggttgcg atactgttta gcacatggag cgatggcgat
     5701 tccggtttac tagcgtcgtg aaacctaagg acaccatttg gaaagcctgt taacccttcc
     5761 tctggctggt gaggccagag gaaggatttt acaaattacc gatactcacc tgtttgcaca
     5821 aaagcacgaa gccctgttag gggtaaacac ctgggaaagc taccaggcgg tgctggaggc
     5881 gattcgtcca caccagcacg aattcgacct gattgtcgcg acaggtgatt tagcgcagga
     5941 tcaatcctct gcggcctatc agcatttcgc tgaaggcatc gcaagttttc gtgcgccctg
     6001 cgtctggctg ccgggcaacc acgatttcca gcccgcgatg tacagcgcgt tacaggatgc
     6061 gggtatctcc ccggcgaagc gggtgtttat tggtgagcaa tggcaaatcc tgttgctgga
     6121 tagccaggtg tttggcgtgc cgcacggtga gctgagcgag tttcagcttg agtggctgga
     6181 acgtaaactg gccgatgcgc cagaacgcca tacgttgctg ctgctgcatc atcatccgct
     6241 acctgcgggt tgtagttggc tcgatcaaca cagtctgcgt aacgcgggcg aactggatac
     6301 cgtgctggcg aagtttccgc acgtcaaata cttgctgtgc ggtcatattc atcaggagct
     6361 ggatctcgac tggaacggtc gccgcctgct ggcaacgccg tcgacctgtg tgcagtttaa
     6421 gccgcactgt tccaacttta cgctggatac tatcgcgccc ggctggcgta ctctcgagtt
     6481 acatgctgat ggcacgctga ccaccgaggt gcatcgcctg gcggacacac gtttccaacc
     6541 tgataccgct tcagaaggct actgatgtct acgcttcttt atttacacgg tttcaacagc
     6601 tcgccgcgct ctgcaaaagc gagcttgtta aaaaactggc tggcggaaca tcaccctgac
     6661 gttgagatga tcattccgca gttgccgccg tatccttccg acgcggcaga actgctggaa
     6721 tccattgtcc tggaacatgg cggcgattcg ctgggtattg tcggttcgtc actgggggga
     6781 tattacgcca cctggttgtc gcaatgtttt atgctgcccg cagtggtggt aaacccggcg
     6841 gtacgcccgt ttgaattgct gacggactat ctcggtcaga acgagaaccc ctacaccggg
     6901 cagcaatatg tgctagagtc acgccatatt tacgatctca aagtcatgca gattgacccg
     6961 ctggaagcgc cggatttgat ctggctgctg caacagacgg gagatgaagt gctggattac
     7021 cgccaggcgg tggcgtacta cgcttcctgc cgccagactg tcatagaagg cggcaaccac
     7081 gcattcacgg gcttcgaaga ttatttcaac ccgatcgtcg attttcttgg tctgcaccat
     7141 ctctgacgat caactcgaat tgctaactta aaccatgacg caaacttata acgctgatgc
     7201 cattgaggta ctcaccgggc ttgagccggt tcgccgccgt ccggggatgt ataccgatac
     7261 cactcgccct aaccatttgg ggcaagaagt tattgataac agtgtcgatg aagcactggc
     7321 gggtcacgca aaacgcgtgg atgtaatctt acatgctgac cagtcgttag aagtgattga
     7381 cgatgggcgc gggatgccgg tggatattca cccggaagaa ggagtaccgg cggttgaact
     7441 gattctttgc cgtctgcatg caggcggtaa attctctaac aaaaattacc agttctctgg
     7501 cggcctgcat ggcgtgggga tttcggtagt taacgccttg tcgaagcgcg tagaagttaa
     7561 cgtgcgccgc gatggtcagg tttataacat cgcctttgaa aatggcgaaa aggtgcagga
     7621 tttacaggtt gtcggcactt gcggtaaacg caataccggt accagcgtgc acttctggcc
     7681 ggatgaaacc ttctttgaca gcccgcgttt ttctgtttca cgcctgacgc atgtgctgaa
     7741 ggccaaagca gtactgtgcc ccggcgttga gattaccttt aaagatgaga tcaacaacac
     7801 cgaacagcgc tggtgctatc aggacggtct gaatgattac ctggcggaag cggtaaacgg
     7861 tttaccgacg ctgccagaaa aaccgtttat cggtaatttc gctggcgata ctgaagctgt
     7921 ggactgggcg ctactgtggc tgccggaagg cggtgaactg ctgaccgaaa gctacgtcaa
     7981 ccttatccca acgatgcagg gcggtaccca tgttaatggt ctgcgtcagg gcctgttgga
     8041 cgcgatgcgt gagttctgtg aataccgcaa cattctgccg cgcggcgtga agctgtcggc
     8101 ggaagatatc tgggatcgct gcgcctatgt tctgtcggtt aaaatgcagg atccgcagtt
     8161 tgccgggcag accaaagagc gtctctcttc gcgtcagtgt gcggcattcg tttcgggcgt
     8221 ggtgaaagat gccttcatcc tgtggctgaa ccagaacgtt caggcggcgg agttgctggc
     8281 agagatggcg atttccagcg cccagcgtcg tatgcgtgcg gctaaaaaag tggtgcgtaa
     8341 aaagctgacc agcggcccgg cgctgcctgg caaactggct gattgtaccg cgcaggacct
     8401 taaccgtacc gaactgttcc tcgtggaagg tgactccgca ggcggatctg ccaagcaggc
     8461 gcgcgatcgt gaatatcagg cgatcatgcc gctgaaaggt aagatcctta acacctggga
     8521 agtttcttcc gacgaagtgc tggcctcgca ggaagtgcat gatatttcgg tagcgatcgg
     8581 tatcgatcct gacagcgacg atctgagcca gcttcgttat ggcaaaatct gtatcctcgc
     8641 ggatgcggac tctgatggtc tgcacattgc cacgctgctc tgcgctttgt tcgtaaaaca
     8701 cttccgcgcg ttggtgaaac acggtcacgt ttacgtcgca ctgccaccgc tctaccgtat
     8761 tgatctcggg aaagaggttt attacgcgct gacggaagaa gagaaagagg gcgtacttga
     8821 gcaattaaaa cgcaagaaag gcaagccgaa cgtccagcgt tttaaaggtc tgggggaaat
     8881 gaacccgatg caattgcgtg aaaccacgct tgatccgaac actcgccgtc tggtgcagtt
     8941 gactatcgat gatgaagacg atcagcgtac tgacgcgatg atggatatgc tgctggcgaa
     9001 gaaacgctcg gaagatcgcc gcaactggtt gcaagagaaa ggcgacatgg cggagattga
     9061 ggtttaaagg aaagaacatt gcccggtcag gatggccggg caaacaaggc aggattaaat
     9121 ccctggctgc agaatacgga tattcatctc cagcacgtcg ccttttacgg ctttgctgta
     9181 cgccttcatg tgcggggttt gcagatgcgc ttcaaggtgc gcgatgcttt cccactgctc
     9241 aatcatcacg atagaatccg gtgccataga ctggaaactc acgccagcag cgcaatccac
     9301 cattggcgca tagccgtggc aaccttcttc tttcagtacg gttggaacga ttttagcaaa
     9361 ctgatccaat accgcctgac ggtgatgttg accaggacga gtacggattt ctgcgattac
     9421 ggtaagcatg gttaactcct tctaaagcct gagctctagt taaccaaaaa tttccacaag
     9481 atgcttgcga tattcttcag tatagcgggg aacatcaggc atttttatca cgtcattagc
     9541 gataaatgtc ggcagcggtt ccataccgag gaattggttt gctttatgga acggcagata
     9601 cacaccgtca acgccaacgc cgtggaagaa ctgatctttt tcggtgaagg cttccattgg
     9661 tgcgttccag gttagagaaa gcatatattt tttgccctgt accaggccgc cggaaccgta
     9721 ttttttcgac ggatctttgc gggtacgacc atcgctggca tacagagtcc cgtgaccttc
     9781 ggtgaataca tcatcaatat attttttcac tgtccacggc gcgcccatcc accagcctgg
     9841 catctgccag atcaccacat cagcccagag aaagttttgt acttccgctt tgacattgta
     9901 gtcgctgtcg gcgcgaacga tgcggacatc atgcccgagg tcgcgcagtg tgccatccgc
     9961 gacttcggtc agggtgtcgt tcagttgacc attggagtgg gcgaattttt tcgcgccgtt
    10021 gataatcagg atgttgctca ttttttaacc tcaaggtgag acgtttgtcg actatagtac
    10081 gtcacagagc ggtacggtga aattagcaaa atgtgcaaag tcttttgcga atttagcaaa
    10141 agtctgatct agctctgact caccgatgca ggataaatat tttctgaaaa tgacaatact
    10201 gatagccatt cctcataatt cttactcgta ccggaataat ttacacgttt ggttcggttt
    10261 ttttggggat tgaaaatatg gctttgaata agtaaaagat gaaaatatca acgtgtcatg
    10321 ttgataatta taaatggatt tttatgagcg tactgatata tgttaaagca gaaaattaaa
    10381 acaatatttg aagcattgct atatataatg cttacttatt ggttaataga ttcttttttt
    10441 gctttcaata agtatgactg gatgcttgag tcgggaggca atatttgcag tataccttcc
    10501 gtttcaggcg aagatcgtat attgcaagca atgattgcag cattttttct tcttacgcca
    10561 cttattattc ttatcttaag aaaactcttt atgcgggaaa tgttcgagtt ttggctatat
    10621 gtattctcgc tcgttatttg tctggtttgt ggttggtggc ttttttgggg acggtttata
    10681 ttttgctatt agtaaaatct atgagagtcg ttttaacggc tctcatagac agagaagtta
    10741 ccagcttacc ttcgcctcaa atccaccttg ttccgcattc ccaaactcaa cattcatgtc
    10801 atgcagcttg gcgattcgct ggacaatcga tagcccaagt ccactgccgg tagcggtttg
    10861 tccgggtggg cgatagaagc gttcgccaat tcgcgccagt gcctctggtg tcacaccggg
    10921 gccgttatcc ctcacgatga aattatcagc attcagcgtg acgtctatca cgctgccctg
    10981 tggactgtag cgcacggcgt tatccagcaa atttcgcacc aacaaactta gcaatagcgg
    11041 ttgtccggtg cgtttgatgc cgtgggcatt gagtgtcagt cgcacgtcaa ttttcgcctg
    11101 ctgcgccgtg tggtaaatat ccatcaccga cgattgtagg agatcttcaa gcgggatctc
    11161 cgcgacgtcc tgaaggttat ccagtgagtc cagccgcgat agcgtgagca gttgatcaac
    11221 cagacgagta gcgcgatcga tcccggaatg taattggagc agtgcttttt tccgcgcctg
    11281 cggatcatcg tcagagagct gcgcaacttc ggtttgcact ttcagcgctg ttaacgggct
    11341 acgtagttcg tgagctgcat cggaggtaaa gcgtcgttct cgaaccatca tcgcatgtgt
    11401 gcgggcgaac agttgattta gcgactcaac cagtggccgc acttcgctgg gtacgccagt
    11461 cgcgtttagt ggtttttccg agtcagggtc acgcatacgt agtgccagcg ccagtttgtt
    11521 cagcggcgcg agttcacgac ccagtagtac catcatgatg attaacataa tcggcagtgc
    11581 gagcagccac gggatcaatt gcccggcaac aatcgccagc gccatgtctt cacggtattc
    11641 ccattcctgg ccaacaacga tgcgatattt gccatcaggt gaggtcatcc agacaaaacg
    11701 ccaaggatcg tcttcaccga tcagttgccc gtcagcaaaa ccttcccgtt gatagctata
    11761 gggaatatct tctccgttat cgccatcatt aaggaccatt ctgccgtcgt gggtaaagat
    11821 ggcaaaggtc agcgcatcgt catcaacatg accgtgtttt aatttatttg gcgtctgtgc
    11881 catgcgatcc gccgcgttga tttcgttgag atcgggcgta cttaaccgtt tggcaaacag
    11941 catcagttgg gtgtcgaaca attcatcgac gttatccgtt gtttgtttcc aggcgacaaa
    12001 gctggaaagc agccaggtca ccgaggccag aattaaaaag attagcgtca gcctgacgcg
    12061 cagactaaga cgttgggtaa atttcatttc tcacctaatg tgtagccaat accatgcacg
    12121 gtacgaataa aatcactact gagtttgcgt cgcagatgat gcacatgcac ttcaacggca
    12181 ttactggtga cctcttcgtc ccaggtatac agtttctctt caatcagttt gcgcggcagt
    12241 acccgaccag cgttacgcat cagtaattcc agcagggcaa attcttttgg tttcagtgtt
    12301 aagggttcgc cagccagcgt ggcgatacgt ttgccggggt cgagcatgac gttaccgtgg
    12361 cgcagctcgt tgctggcctg gccgttggtt cggcgcatca gagcctccag cctggcggcg
    12421 acttctatca acgcaaaagg tttacacaga taatcgtcag ctcccagacg cagcccttct
    12481 acacgttccg ctaacgcatc acgcgcggtc aggttcagta ccggctcacg ctgacctttc
    12541 tctcgccatt cgcgcaaaat atcgcgacca tccatgcctg gtaaggttaa atccaggatc
    12601 accgcatcat aaggtgcgct ataaagcgcc tcttttccct gacgaccttg tgtaaaccag
    12661 tcgacactaa aacccatttt actaaggcca gttttgatgc cgtcaccaat cagcatgtca
    12721 tcttctatca gtaaaattcg cattttttca tccctgcgat aaccgtattt gctgccagta
    12781 aaccgtgttg taacgcgctc tgtacagcat taaattttat ttttttccct taagaagttg
    12841 ttaaggacta tcttgttaaa tgctcggaaa cagacattaa agggagtaat aaacatgaaa
    12901 aaattcgcag cagtaatcgc agtaatggcc ctgtgcagtg caccggtgat ggcagcagag
    12961 cagggcggtt tttctggccc atcggcaacg caaagtcagg ccggaggatt tcaggggccg
    13021 aacggcagcg taacgactgt agaaagcgta aaatccctgc gtgacgacac ctgggtaacc
    13081 ctgcgcggca atatcgttga acgcatctct gacgatctct acgtgtttaa agatgccagc
    13141 ggtactatca atgttgatat cgatcacaaa cgctggaacg gtgtgacggt gacgccgaaa
    13201 gatacggttg agattcaggg tgaagtcgat aaagactgga attctgttga aattgacgtc
    13261 aaacagatcc gcaaagtaaa tccgtaattg ttaccgctcc cgggacgcgt tcccgggaat
    13321 aatttcgcag ggaggcaaaa tgacaaacct gacactggat gtaaacatta tcgatttccc
    13381 atcaatccct gtggcgatgt tgccgcaccg ctgtagccct gagttgctca actacagtgt
    13441 ggcgaaattt atcatgtggc gtaaagagac ggggctttct cctgttaacc aaagccagac
    13501 ttttggcgtc gcctgggacg accctgccac caccgcaccg gaagcgtttc gctttgatat
    13561 ctgcggcagc gttagcgaac cgattcccga taatcgttat ggtgtgagca atggtgaact
    13621 taccggtgga cgttatgccg tggcccgcca cgttggcgag ctggacgata tttcacacac
    13681 gatatggggc attattcgcc actggctgcc tgcaagcggc gagaaaatgc gtaaagcacc
    13741 gattctgttt cactacacca atcttgccga aggggtgaca gagcagcgac tggaaacgga
    13801 tgtttatgtg ccgttggcgt gatggggaat ctttaccgga gtgtgagcca aatcactcgc
    13861 gataagtttg agcgaacaat gtcacaggcg tattgttgtt gggcttataa tttctccggt
    13921 aaggaatacc atgtatacgc gaaatttatt atccggtgcg ccgtaagccg tctcgggggt
    13981 atccatgagc aagccgcact tatggcagga ggcgcggggc actggtggag acacagctaa
    14041 actgggctga accgtatact tacagtcgac tactttgatg ctttaggctt ccctggactc
    14101 gcataacctc aacttctcga atcgcccgga gcggaccccc atgccgagtg gtgtggcatg
    14161 ggcgcaacct tgtgctgccc tctatgccga tcaagtctac agcaaagagt tgtatatcaa
    14221 ggcacattga tgcctttatc gccgtcggag cacgtccggc ggcgctattc acgctgatct
    14281 cctgtgactc gacgcggcag ataatgtagt atctccggca atattgcccc tttgaaggct
    14341 ggcgaataag ttgaggaatc agaattaatg agcgatatgg cagagcgcct tgcgctacat
    14401 gaatttacgg aaaacgccta cttaaactac tccatgtacg tgatcatgga ccgtgcgttg
    14461 ccgtttattg gtgatggtct gaaacctgtt cagcgccgca ttgtgtatgc gatgtctgaa
    14521 ctgggcctga atgccagagc caaatttaaa aaatcggccc gtactgtcgg tgacgtactg
    14581 ggtaaatacc atccgcacgg cgatagcgcc tgttatgaag cgatggtcct gatggcgcag
    14641 ccgttctctt accgttatcc gctggttgat ggtcagggga actggggcgc gccggacgat
    14701 ccgaaatcgt tcgcggcaat gcgttacacc gaatcccggt tgtcgaaata ttccgagctg
    14761 ctattgagcg agctggggca ggggacggct gactgggtgc caaacttcga cggcactttg
    14821 caggagccga aaatgctacc tgcccgtctg ccaaacattt tgcttaacgg caccaccggt
    14881 attgccgtcg gcatggcgac cgatattcca ccgcataacc tgcgtgaagt agctcaggcg
    14941 gcaatcgcat taatcgacca gccgaaaacc acgctcgatc agctgctgga tatcgtgcag
    15001 gggccggatt atccgactga agcggaaatt atcacttcgc gcgccgagat tcgtaaaatc
    15061 tacgagaacg gacgtggttc agtgcgtatg cgcgcggtgt ggaagaaaga agatggcgcg
    15121 gtggttatca gcgcattacc gcatcaggtt tcaggtgcgc gcgtactgga gcaaattgct
    15181 gcgcaaatgc gcaacaaaaa gctgccgatg gttgacgatc tgcgcgatga atctgaccac
    15241 gagaacccga cccgtctggt gattgtgccg cgttccaacc gcgtggatat ggatcaggtg
    15301 atgaaccacc tcttcgctac caccgatctg gaaaagagct atcgcattaa cctcaatatg
    15361 atcggtctgg atggtcgtcc ggcggtgaaa aacctgctgg aaatcctctc cgaatggctg
    15421 gtgttccgtc gcgataccgt gcgccgccga ctgaactatc gtctggagaa agtcctcaag
    15481 cgcctgcata tcctggaagg tttgctggtg gcgtttctca acatcgacga agtgattgag
    15541 atcattcgta acgaagatga accgaaaccg gcgctgatgt cgcggtttgg ccttacggaa
    15601 acccaggcgg aagcgatcct cgaactgaaa ctgcgtcatc ttgccaaact ggaagagatg
    15661 aagattcgcg gtgagcagag tgagctggaa aaagagcgcg accagttgca gggcattttg
    15721 gcttccgagc gtaaaatgaa taacctgctg aagaaagaac tgcaggcaga cgcgcaagcc
    15781 tacggtgacg atcgtcgttc gccgttgcag gaacgcgaag aagcgaaagc tatgagcgag
    15841 cacgacatgc tgccgtctga acctgtcacc attgtgctgt cgcagatggg ctgggtacgc
    15901 agcgctaaag gccatgatat cgacgcgccg ggcctgaatt ataaagcggg tgatagcttc
    15961 aaagcggcgg tgaaaggtaa gagtaaccaa ccggtagtgt ttgttgattc caccggtcgt
    16021 agctatgcca tcgacccgat tacgctgccg tcggcgcgtg gtcagggcga accactcacc
    16081 ggcaaattaa cgttgccgcc tggggcgacc gttgaccata tgctgatgga aagcgacgat
    16141 cagaaactgc tgatggcttc cgatgcgggt tacggtttcg tctgcacctt taacgatctg
    16201 gtggcgcgta accgtgcggg taaggctttg atcaccttac cggaaaatgc ccatgttatg
    16261 ccgccggtgg tgattgaaga tgcttccgat atgctgctgg caatcactca ggcaggccgt
    16321 atgttgatgt tcccggtaag cgatctgccg cagctgtcga agggcaaagg caacaagatt
    16381 atcaacattc catcggcaga agccgcacgt ggcgaggatg gtctggcgca actgtacgtt
    16441 ctgccgccgc aaagcacgct gaccattcat gttgggaaac gcaaaattaa actgcgtccg
    16501 gaagagctac agaaagtcac tggcgaacgt ggacgccgcg gtacgttgat gcgcggtttg
    16561 cagcgtatcg atcgtgttga gatcgactct cctcgccgtg ccagcagcgg tgatagcgaa
    16621 gagtaatatg ccggggaggg cgctgtttcc tcccgctagc aaggaacgcc ggatgaaacg
    16681 ttaagtcgtc atccggcagc gtattcaacc cccggaaacg ataaaaattc ccttgaaacc
    16741 gttgtttatt catgcgttgc gattaacaat acgcttttcc agagagcggc ttttaacaat
    16801 gccctaacct gatttcaggt gacgtacaat gccagtctct cagaccttca gagggtgtta
    16861 tgctatatat ctttcgtctt attattaccg tgatttacag catcttagtc tgtgtattcg
    16921 gctccattta ctgccttttc agcccgcgta acccgaaaca tgtggccacc tttgggcata
    16981 tgtttggccg tcttgcgcca ctgttcggcc tgaaagttga gtgccgcaaa cctgcagatg
    17041 ctgaaagcta cggcaatgct atctatatcg ctaaccacca gaacaactat gacatggtga
    17101 cagcatcgaa cattgtgcaa ccgccgacgg tgacggtagg taaaaagagc ttgctgtgga
    17161 tccccttctt cgggcagttg tactggttaa ccggcaactt attgatcgac agaaacaatc
    17221 gcactaaagc tcacggcacc attgcggaag tcgtgaatca tttcaaaaaa cgccgtattt
    17281 ccatctggat gttcccggaa ggaacccgca gccgtggtcg cggcctgcta ccgttcaaga
    17341 ctggagcatt tcacgcggca attgcggcgg gcgtcccgat tattcccgtg tgcgtctcta
    17401 caacttcgaa taagattaat cttaatcgac tgcacaacgg tctggtgatt gtcgaaatgc
    17461 tgccgccaat tgacgtcagt cagtatggca aagatcaggt tcgtgagctg gctgcccatt
    17521 gtcgttcgat aatggaacaa aaaatcgccg agctcgataa agaagtcgca gaacgcgaag
    17581 ccgccggaaa agtttaagtc ggcaatctgt atttttgcgg ggaacacttt cctgcacggt
    17641 attactttag ccagttttac atggagcaaa tatgtcactc agtcggcgtc agttcattca
    17701 ggcatcgggg attgcacttt gtgcaggcgc tgttcccctg aaggccagcg cagccgggca
    17761 acagcaaccg ctacccgttc cgccgctact tgaatctcgc cgtgggcaac cgctgtttat
    17821 gactgtacaa cgtgcgcact ggtcatttac gccagggaca cgcgcgtcgg tctggggaat
    17881 caatggtcgt tacctggggc cgactatccg cgtctggaag ggcgacgatg ttaagcttat
    17941 ttacagcaac cgcctgacag aaaatgtctc aatgacggtg gccgggctac aggtaccagg
    18001 cccgctgatg ggcggtccgg cacggatgat gtcgccaaac gctgactggg cacccgtact
    18061 gcctattcgc cagaacgcag ctactctgtg gtatcacgcc aatactccca accgcacggc
    18121 tcagcaggtc tataacggcc ttgccggaat gtggctggtg gaagatgaag tcagcaagtc
    18181 gctgcctatc cccaaccatt atggtgtgga tgattttccg gtcattatcc aggataaacg
    18241 gctggataac tttggtacgc cagaatacaa cgaaccggga agcggcggct ttgttggtga
    18301 tacgctgctg gttaacggtg tacaaagccc gtacgttgaa gtctcgcgtg gctgggtgcg
    18361 cttgcgactg ctgaacgcgt cgaactctcg tcgctatcaa ctacagatga gcgatggtcg
    18421 cccgttacat gtgatttctg gcgatcaggg attcctgcct gctcctgtat cggtgaagca
    18481 actttcgctg gcaccgggcg agcgccgcga gattctggtg gatatgagca acggcgatga
    18541 agtgtcgatc acctgtggcg aagcggcgag cattgttgat cgtattcgcg gcttctttga
    18601 gccatccagt attctggttt ctaccttggt gctaacgctg cgcccaaccg gccttctgcc
    18661 gctggtcaca gacagtcttc cgatgcgctt gctgccaact gaaatcatgg ctggttcgcc
    18721 aattcgcagt cgcgatatca gtctgggtga tgacccgggt attaatggac agctgtggga
    18781 cgtcaaccgt attgatgtca ccgcgcagca aggaacgtgg gaacgctgga cggtacgcgc
    18841 ggacgagccg caagcgttcc atattgaagg cgtaatgttc cagatccgta acgtgaatgg
    18901 cgcgatgccg ttcccggaag acagaggctg gaaagatacc gtttgggttg acggacaagt
    18961 ggagctgctt gtttatttcg gtcagccttc ctgggcgcac ttcccgttct acttcaacag
    19021 tcagacgctg gaaatggcgg accgtggctc gattgggcaa ctgttggtca atccggtacc
    19081 gtaatcttca tactcgcccg aggagggcga gtatggaata cctttcgatt tctctttcgt
    19141 tccggcttat aatccccggc ctttgattat ttttttatca tttctggaag caaaatgagc
    19201 tctatctccc tgatccaacc ggatcgcgac ctgttctcct ggccgcagta ctgggccgcc
    19261 tgttttggac cggcaccgtt tttgccgatg tctcgtgaag agatggatca acttggctgg
    19321 gatagctgcg acatcatttt ggttactggc gacgcgtatg tcgatcaccc aagcttcggg
    19381 atggcgattt gcggtcgtat gctggaagcg cagggctttc gcgtcgggat catcgcccag
    19441 ccggactgga gcagcaaaga cgactttatg cgtctgggta aaccgaatct gttttttggt
    19501 gttactgctg gcaacatgga ttcgatgatc aaccgctata ccgccgatcg ccgtttacgt
    19561 catgatgatg cctacacgcc agataatgtc gcgggtaagc gaccggatcg cgccacgctg
    19621 gtttataccc agcgttgtaa agaggcgtgg aaagatgtgc cggtgatcct cggcggtatt
    19681 gaggctagcc tgcgccgtac cgcgcattat gattactggt ccgataccgt gcgccgttcc
    19741 gttctggtag attcgaaagc cgacatgctg atgtttggta acggtgaacg tccgctggtg
    19801 gaagtagcgc atcgtctggc gatgggcgag ccgattagtg aaatccgcga tgtgcgtaat
    19861 accgcaatta tcgtaaaaga ggcgctgcct ggctggagcg gtgtggattc cacccgtctt
    19921 gataccccag ggaaaatcga cccaatcccg catccgtatg gcgaagattt gccgtgtgcg
    19981 gataacaaac ctgttgcgcc gaaaaagcag gaagcgaaag ctgtaatcgt gcagccaccg
    20041 cgccagaaac cgtgggaaaa aacctacgta ttgctgcctt ctttcgagaa agtgaagggc
    20101 gataaagtgc tgtacgccca tgcttcgcgc attctgcacc acgaaaccaa cccaggctgc
    20161 gcccgcgcat tgatgcaaaa acacggtgac cgctatgtgt ggatcaaccc gcctgctatc
    20221 ccgctttcta ccgaagagat ggactgcgtt tttgcgctgc cgtacaagcg cgtgccacat
    20281 ccggcctatg gcaatgcccg tattccggct tacgaaatga tccgtttttc ggtcaacatt
    20341 atgcgcggct gctttggcgg ctgttctttc tgttctatta ccgagcacga agggcgcatt
    20401 attcagagcc gttcggaaga ttcgctcatt aatgagatcg aagcgatccg cgacaccgtt
    20461 ccaggtttta cgggcgtgat ttccgatctc ggtgggccaa ctgccaacat gtatatgttg
    20521 cgctgcaaat cgccacgcgc tgaacaaact tgtcgccgtt tgtcgtgcgt ttatccggat
    20581 atttgtccgc acatggacac taaccacgaa ccgacgatca acctctatcg ccgtgcgcgt
    20641 gatctgaaag gcattaaaaa aatccttatc gcctcaggcg tacgttatga catcgccgtg
    20701 gaagatccgc gctatatcaa agagttggcg acccatcacg tcggcggtta tctgaagatt
    20761 gctccggaac ataccgaaga agggccgttg tcgaagatga tgaagccggg catgggcagc
    20821 tatgaccgct ttaaagagct gttcgatacc tactcgaaac aggcaggtaa agagcagtat
    20881 ctgatcccgt atttcatctc tgcgcacccc ggtacgcgtg atgaagatat ggtgaatttg
    20941 gcgctgtggc tgaaaaagca tcgtttccgt ctcgaccagg tacagaactt ctacccatcg
    21001 ccgctggcga actcaaccac catgtattac accgggaaaa aaccgctggc gaagattggt
    21061 tataagagcg aagacgtctt cgtaccgaag ggcgacaaac agcgtcgttt gcataaagcg
    21121 ttgttgcgtt accacgatcc ggcaaactgg ccgttaatcc gccaggcgct ggaagcgatg
    21181 ggcaaaaagc atctgattgg cagccgtcgc gattgcttag tgcctgcgcc aaccattgaa
    21241 gagatgcgtg aagctcgtcg ccagaaccgc aatacccgtc cggcgttgac taaacatacg
    21301 ccgatggcga ctcagcgtca gacgcctgct acggcaaaaa aagcgtcgtc tacgcaatct
    21361 cgtccggtga atgctggtgc gaagaaacgc cctaaagcgg cggttggacg ttaaaaaaaa
    21421 tccccggcaa ctgacatgct gccggggatt tttttatcat tctgcgtaca tacgcaagca
    21481 gcgcaggcaa tcaaaatagt cctgtgcatc aagacgcgta ttggctctgt tagcttcgga
    21541 tttggcagca tagatttgtg ggcgtagtgc tttcatatcc tgctcaagcc gggcgatttt
    21601 ggcatttaat gtttgtacat tgctcgccag ttgattgacc ttttgctcat tgacgcagcc
    21661 actgagcaag caggttgcca gaacaacagc tcccacggtg aaaatcgttt tcatatagtg
    21721 ttccctttta tttattgttc cggctaattg ttatttaatt cagctcattg ctttaatctg
    21781 gcggcggttt ttgcctcaat cagcttaagt aggctgccgc tgttcgggcg gataaaatca
    21841 gcgatttccc acttaccttc gcgccgctgt aaaaccagct cttgctcttc attgttgcca
    21901 ttggtcagcg taaaacgaac atgaatgcgg gcggaatcgg cggttttctc caggactcga
    21961 atttcatcaa cgcgtgaaaa tagcgactct tcgccaaaac agatcggatt gtcgtaaata
    22021 aattgccgtt ctatttgcat atcttcatcg ggattattgg ccgtcttgaa ggtggtttta
    22081 tagaaggcat taaccgcctg gcggtaagag tcggaaacca cccacgtcca cggccagcta
    22141 taatcagcaa tatgttcaaa gagttcagca acaaaggatt ctggctgttc tttctgcaac
    22201 gaagctaaag cggccagcgt tttttcattc tcgctattaa ctgcttgtaa gacgctgcca
    22261 tgattgctga caatatcatc aatgacccaa cgaccatttt ccgccaccat tttcagtgtc
    22321 tgtgtggtct tttctttatc atctttaaag atacgaaagc gcacaacggc atcggcatga
    22381 tcgacgtcag tttgggttat cgctacgctt tctaacacca gatcgccaaa atcctgacaa
    22441 tcacaaaccg gatcataatc cagccagcca atattgcccg gtagtgtaag attgtcgttc
    22501 agggtaagcg cctgttgaat acgcgctgaa gttatcgccc gctctccggt ttcaccgaaa
    22561 taaggcgtac tggcatctga tttatagttc tgataaatct gccggactgt ttgttctacg
    22621 gtctgtgaag gaggctgcgc gtgtacggta aaacttgcca gggctgctaa aaacagaagt
    22681 attattttca tctgtttttc acctctccgt tgcgtttatc ccggatatag atgagttgtt
    22741 cttttaagag cttccggctc tgcatgatga tgtccttata tttggcattc ctgaacaagg
    22801 aagagtaaca acgggcggga cgcgagggga ataaatgatt tctgaaaagt ccggtagcgg
    22861 aacattaccg ccaccgggag aatttgcacg ttagccgccg aactggtcag gatcgggacc
    22921 gagacgcttg ccctgatcga gttttgcaat tttgccgggt tcgtctttgt cgagacggaa
    22981 atcccagaca tcaaagtttt cggcaatacg tgaaggtgtg accgatttcg ggatcaccac
    23041 caggccgcta tccagatgcc agcggataac aatctgcgcc ggggttttgc cgtatttatc
    23101 tgccagatcg cgaatgactt tctgatcgaa aacgcctttt cctccttgcg ctaatgggct
    23161 ccaggattcg gtctggattt tgtgtgtcgc gttccaggcg tgtagctggc gttgttgcat
    23221 cagcggatga agttcgatct ggtttatcac aggcgtcacg ccagtttcat caatcaggcg
    23281 ttgcaggtga tggatctgga agttgcacac gccgatgctt ttgattaatc cctctttttg
    23341 caattcgatc atgcctttcc atgcttcgac ataatggtcg atagcgggaa cgggccagtg
    23401 cattaagtag aggtcgatat aatcaagctg gagttttttc aggctgtcga gcagggcttc
    23461 gcgggggcac ttgtggtcgt cgttccacag cttagtggtg atgaacagtt cttctctgtt
    23521 gactgaggca tttttcaggg ctttgccgac accttcttcg ttcttgtagg ccgcggcggt
    23581 atcaatcgag cgataaccca cttctaacgc tttttgaatg gcggtgatta cttcctcatt
    23641 acttgcttgc cagacgccca gtcccagctg gggcatgaca ttgccatcct gtagcttaat
    23701 aacggttgga ttagccatac gttcctcctt tatatgaact caccggagca ggctccggtg
    23761 aggtgttgtg aacttaagtc tggacgaaat gcccgaaaac gaaagtttga ggcgtaaaaa
    23821 gcttagcggg cgactttgta tatacggcgg ctgacatcca acgtaatgtc atgattttcg
    23881 cccagttggg tcatgccgtg ctcttccagt tttttcagca aagccgggat ggagctgccg
    23941 tccagaccgt agtcggagag gtgggtcggc acgcctaatt gctcaaagaa attgcgggtt
    24001 gcggcaatcg cggcgtcaat acgctcatcg tcggaacctt cagtgatgtt ccagacgcgt
    24061 tcagcatatt gcagcagctt agcgcgcttg gtctcgcgtt tttcattcca cagtgcaggc
    24121 aggacgatag ccagtgtttg cgcgtgatcc agaccgtgca tcgcagtcag ttcgtggccc
    24181 agcatatgcg ttgcccagtc ctgcggtacg ccagcgccaa tcaaaccgtt cagcgcctga
    24241 gtcgccgccc acatgacgtt ggcgcgcaca tcgtagtttt ctggctcttt cagggctttc
    24301 ggaccatctt cgattagcgt cagcaaaatg ccttctgcga aacggtcctg aattttggca
    24361 tcaaccggtt tggtaacata ctgttccacg gtgtgtacaa aggcgtccac tacgccgtta
    24421 gccacctgac gcggcggcag ggtgtaggta taaaccggat cgagcacggc aaataccggc
    24481 tgaacatgtg cagaatggaa cgcctgcttg tcgcctgtgg ttttacggga gatcaccgcg
    24541 cctgcgttgg attctgaacc ggttgctggc agcgtcagca cacagcccat cgggatggcg
    24601 cttttaatct ctttaccgcc cgtttgcaga atgtgccacg gatcgatatt ttccggatag
    24661 ttagccgctg cggcgataaa tttggtgccg tccagtacag aaccgccgcc aaccgccagc
    24721 aggaaagtca ctttctgttc gcgaaccagt ttcacggcgt tcatcagcgt ttcataagcc
    24781 gggtttggct caataccgcc aaattccagc acgtccatgc ctttcagggc atccagaact
    24841 tgatcgagaa cgccggtttt tttcacgctg ccgccaccgt aggtaatcaa tacgcgagca
    24901 tcgtgaggaa tttgttcgcg taaaccagcg attgcgcctt taccaaacag aatgcgggtt
    24961 ggggtgtgca gattaaagtt gttcattact tgctcccttt gctgggccaa tatgagggca
    25021 gagaacgatc tgcctgatgt ttttcattgt gatcgccagc gccctggctc tcaatgctca
    25081 tttctgccaa tgtcttgcct atttctccag agtgctggag aaatgctaca aaattgcgca
    25141 caatcaaatt gccgcattat tcctaagaaa ttacgcgata tgaaacgtga agagatttgt
    25201 cgcttgctgg cggataaagt taataaactg aaaaataaag aaaatagttt gtcagaactg
    25261 ttgcccgatg tgcgtttgtt gtatggcgag acgcctttcg cacgtacacc ggtgatgtac
    25321 gagcctggca tcataattct cttttccggg cataaaatcg gttatatcaa t
//
LOCUS       DADLZM010000048        25088 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLZM010000048 DADLZM010000000
VERSION     DADLZM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.048"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(130..1650)
                     /locus_tag="JNY73_001919"
     CDS             complement(130..1650)
                     /locus_tag="JNY73_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8047948.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKSKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            1741..2472
                     /gene="tonB"
                     /locus_tag="JNY73_001920"
     CDS             1741..2472
                     /gene="tonB"
                     /locus_tag="JNY73_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY8047949.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRAL
                     SRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWR
                     YEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(2512..2910)
                     /gene="yciA"
                     /locus_tag="JNY73_001921"
     CDS             complement(2512..2910)
                     /gene="yciA"
                     /locus_tag="JNY73_001921"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY8047950.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3015..3554)
                     /locus_tag="JNY73_001922"
     CDS             complement(3015..3554)
                     /locus_tag="JNY73_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY8047951.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(3584..4327)
                     /locus_tag="JNY73_001923"
     CDS             complement(3584..4327)
                     /locus_tag="JNY73_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY8047952.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4355..4444)
                     /locus_tag="JNY73_001924"
                     /pseudo
     CDS             complement(4355..4444)
                     /locus_tag="JNY73_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4684..5322
                     /gene="ompW"
                     /locus_tag="JNY73_001925"
     CDS             4684..5322
                     /gene="ompW"
                     /locus_tag="JNY73_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY8047953.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5382..5888)
                     /locus_tag="JNY73_001926"
     CDS             complement(5382..5888)
                     /locus_tag="JNY73_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY8047954.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(5934..6434)
                     /locus_tag="JNY73_001927"
     CDS             complement(5934..6434)
                     /locus_tag="JNY73_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY8047955.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6520..6699)
                     /locus_tag="JNY73_001928"
     CDS             complement(6520..6699)
                     /locus_tag="JNY73_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY8047956.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(7080..7886)
                     /gene="trpA"
                     /locus_tag="JNY73_001929"
     CDS             complement(7080..7886)
                     /gene="trpA"
                     /locus_tag="JNY73_001929"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY8047957.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(7886..9079)
                     /gene="trpB"
                     /locus_tag="JNY73_001930"
     CDS             complement(7886..9079)
                     /gene="trpB"
                     /locus_tag="JNY73_001930"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY8047958.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(9091..10449)
                     /gene="trpCF"
                     /locus_tag="JNY73_001931"
     CDS             complement(9091..10449)
                     /gene="trpCF"
                     /locus_tag="JNY73_001931"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY8047959.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(10453..12048)
                     /gene="trpD"
                     /locus_tag="JNY73_001932"
     CDS             complement(10453..12048)
                     /gene="trpD"
                     /locus_tag="JNY73_001932"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY8047960.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(12048..13610)
                     /gene="trpE"
                     /locus_tag="JNY73_001933"
     CDS             complement(12048..13610)
                     /gene="trpE"
                     /locus_tag="JNY73_001933"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY8047961.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            13883..14764
                     /gene="yciV"
                     /locus_tag="JNY73_001934"
     CDS             13883..14764
                     /gene="yciV"
                     /locus_tag="JNY73_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY8047962.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            14761..15381
                     /locus_tag="JNY73_001935"
     CDS             14761..15381
                     /locus_tag="JNY73_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY8047963.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKS
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15482..16354
                     /gene="rluB"
                     /locus_tag="JNY73_001936"
     CDS             15482..16354
                     /gene="rluB"
                     /locus_tag="JNY73_001936"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY8047964.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16394..16984)
                     /gene="cobO"
                     /locus_tag="JNY73_001937"
     CDS             complement(16394..16984)
                     /gene="cobO"
                     /locus_tag="JNY73_001937"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY8047965.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(16981..17739)
                     /locus_tag="JNY73_001938"
     CDS             complement(16981..17739)
                     /locus_tag="JNY73_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY8047966.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            17959..19008
                     /gene="sohB"
                     /locus_tag="JNY73_001939"
     CDS             17959..19008
                     /gene="sohB"
                     /locus_tag="JNY73_001939"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY8047967.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     AWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(19044..19295)
                     /locus_tag="JNY73_001940"
     CDS             complement(19044..19295)
                     /locus_tag="JNY73_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY8047968.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            19675..22272
                     /gene="topA"
                     /locus_tag="JNY73_001941"
     CDS             19675..22272
                     /gene="topA"
                     /locus_tag="JNY73_001941"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY8047969.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVIDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            22482..23456
                     /gene="cysB"
                     /locus_tag="JNY73_001942"
     CDS             22482..23456
                     /gene="cysB"
                     /locus_tag="JNY73_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY8047970.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            23751..23915
                     /locus_tag="JNY73_001943"
     CDS             23751..23915
                     /locus_tag="JNY73_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY8047971.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            23918..24085
                     /locus_tag="JNY73_001944"
     CDS             23918..24085
                     /locus_tag="JNY73_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047972.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            24199..24294
                     /locus_tag="JNY73_001945"
     CDS             24199..24294
                     /locus_tag="JNY73_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047973.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            24458..>25088
                     /locus_tag="JNY73_001946"
     CDS             24458..>25088
                     /locus_tag="JNY73_001946"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HAY8047974.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVG"
BASE COUNT         6334 a         6156 c         6336 g         6262 t
ORIGIN      
        1 tcacagcctc agctaatgag cgagcttcaa aagtgcttaa gtcgggtaat gctttacgga
       61 agtactctgt gaatccgaat aagaagggcc ttattgagac ataaaaaggg gcttcatagc
      121 ccctgaaaat tacagttgat aagcatcaat atccagtagc tctttatcag caatttcaaa
      181 gattggtgac ccatcattta gcggtagcgg gtctgtaggt atggtgattt cagtgtcgtt
      241 ctgtagcgtt atagttactg aacccttacc caatgtaagc ccttttagat gtttggacaa
      301 aattaattga cactcgacac gacccgcttt agttgtcagg tcaatatctt taagctgggg
      361 tagttcctta tcagtgagtg aagccttacg cttcatagta tcaatcgtgg tctgtagttc
      421 tgtgcgcttc tggttaagtt ccttaagctg aatttttagt tcatcaatat catccatctc
      481 aagaagtagg gcggtagctt tcttgattct ggctgtgaca atttcaacct catgctgtaa
      541 cgctgcaatc ttcctctctg attctccatc attccccacg tcaatcatcc ccagaaaatt
      601 atcaatcatc catcggtcaa acggtttacg cttcaacggt ggaacatcac agcgaccgtc
      661 taagtggtta ttgcacctgt agaccccagc atacttactc tttgttccgg taggatggac
      721 actagcccca cactgacacc taaacagacc tttgaagatg ttaatcgtta gtgggttgtc
      781 acttgcaggt gtacggcctt tacgattctt atcaagaatc tcttgagctt tattgaacgt
      841 tgcagcatct atagcggctt cgtagtagtt cggtatagcg gggcggtcat tgcgctgtga
      901 tgctggtagt gtacctatcg ttgtctctga ccttaagata gtacgcacag aatgaacgct
      961 aaatccatta gcatctttac gtcgtgagaa tgaactaatg ttattagcct gtaggtgacg
     1021 tgcaatgtta tgcagacttt caccatctag cagcagcttg aaaatcaagc gtacagtttc
     1081 agcatgttca ttgagtttaa aagtctcagg gtcaatccat ccgggaacat tccttgttaa
     1141 acgttgttca ccagcaaggg caagctttgc acgttgttcc catttctgac taacacggaa
     1201 agacttctgt ttactctcct gatgcgctat gtagctttta acccgctgaa tgattgagat
     1261 tcctaaatca tcggatagag catcccttgt gtagattagc ccatcctgta gagtgtgaat
     1321 ctcgatacca tgagcaataa tgcgattcat caaggcttct gtttcaagat gttcaaggcg
     1381 gcctatgcgg tcgatagctt cgactagtaa aattgtccct tctggaaact cccctgattc
     1441 aatgcgggag agcatatccc caaattgccc tgataagtgt ttacctttgt aaccggatag
     1501 accttcatca ttgaaagatg ccactacctg agcatcgcta ttcttcctta accactgttt
     1561 gaatagctca ttttgtcgtc tggttgaatc agcaccttca gcaccttgtg tggctgatga
     1621 gaatctcaag tatgttatta cggttctcac ggtgataaac tcccatgcaa ttgattatca
     1681 tttaaagatg aacttataaa cgttatggct aatgctacag tgccgaatag ctacaataca
     1741 atgccccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat
     1801 ggtgctgttg tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg
     1861 cctgcgcaac caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc
     1921 gttcagccgc caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa
     1981 ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg
     2041 aagccaaaac cgaagccggt gaaaaaggta caggagcagc caaaacgcga tgtcaaaccc
     2101 gtagagtcgc gtccggcatc accgtttgaa aatacggcac ccgcgcgccc cacatcaagt
     2161 acggcaacgg ctgcaaccag caagccggtt accagtgtgg cttcaggacc acgcgcatta
     2221 agccgtaatc agccgcagta tccggcacga gcacaggcat tgcgcattga agggcaggtt
     2281 aaagttaaat ttgacgtcac gccggatggt cgcgtggata acgtacaaat cctctcagcc
     2341 aagcctgcga acatgtttga gcgtgaggtg aaaaatgcga tgcgcagatg gcgttatgag
     2401 ccgggtaagc caggcagtgg gattgtggtg aatatcctgt ttaaaattaa cggcaccacc
     2461 gaaattcagt aagcagaaag tcaaaagcct ccgaccggag gcttttgact attactcaac
     2521 aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca tacttaaata atgcttctgt
     2581 cgctttatag cgttgcccga ttggttcaga cgctactttt ttcacccaca cttcaatatt
     2641 aatgctgacc gatgtcgtcc ctttctggac acagcgtgca tagcagcaca ccacatcgcc
     2701 gaccgcaacc ggccgtaaga aagtcattcc ttcaacccgc acagtcacta cgcgaccgtg
     2761 ggcaatttct tttgccagaa tagcgccgcc aatatccatt tgtgacatta accaaccacc
     2821 aaagatgtca ccattggcat tggtatcggc gggcatggct aaagtacgta aaacaagatc
     2881 gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg acttaaaagg aaaatcacag
     2941 gcgcgatgct actatgattt tcctgaggag aacagaggag atacagcgcc agccccgaag
     3001 gactggcagt ctggttagga tttatcttcc tgcggcatgt ggcggtagat atagataccg
     3061 cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa agactttaaa gttgacccaa
     3121 atattttgcg gcagccagaa tgcgatgtag atgtttgcca gaccgcaaag gataaagaaa
     3181 acagcccagg ccagatttag cttcgaccat accggttgcg gcagcgtgag ttctttaccc
     3241 agcatccgct gaattagcgg ctttttcatc acccattggc tgactaacag ggctaccgca
     3301 aacagagcat aaatgactgt aaccttccat ttaataaact catcattgtg gaagaacagc
     3361 gtcaagccac cgaagacgac caccagaaca aaagtgatca gggccatctt ctcaacctta
     3421 cgaaagcgaa cccagctata gataagcaca atcgccgtgg cgacgatcag cgctgcagta
     3481 gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg taaaaaatca
     3541 agaaactgct tcattttacg attccgtaat caaatgctta aggttattgg cgaatcaaca
     3601 tatacaggcg gaacagatag atgagcaata tggcagaaat caggttgctc aaggtgttcg
     3661 ccagtacggc accaatttcc ggggttaatg cggcaaaaga agaggcaaaa agcagcaaca
     3721 gtgtttttgc cagcaaccag ctcagtactg cgggtgccac cagacgcata ttcgcccaag
     3781 tcagccgcat actgctacgc atcgaggcaa aaatgcccat tttgtcctgt accagcatca
     3841 ccggtgccag ggccagtaaa atggccatga taattcccgg aacgaccacc agcatgatgc
     3901 caatctgtac taaaagggta gtcagaaaaa tcagaataaa taacttgggc aatatcggcg
     3961 cactggcacc aatagctcgc agcgcacaga ctctctgacc cgcagacacc agctggataa
     4021 ttaatattac gcctccggcg agaatggcgt taccgattaa ttctgaaaaa gtggacgccg
     4081 ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat attctgaacc aggtcgaaca
     4141 acccactact gccgctaacg ggcacgccgt cattgagctg cgcaagctgt gcatcactgg
     4201 gtgagaaaac atgccctaac accactgtga taaacgcaca tagcaacgat accaacagaa
     4261 tggtcataaa ttgattacgg aagaaatttc cggtgtcacg gtatacggac tgcgccgtga
     4321 tagacatgca ctctccttga gttttatgca ggtgttaatt agcgggcaat tgtaccctgg
     4381 ttaagccttg ggtggcagca tcaggtcttg tatggaaaag catatctttg taaagcggag
     4441 gtaattgctg gccttcaggc gtgggcaaac cgcggcaagt ggcgatcgag ttaatatttg
     4501 cgtagagaaa atatttaaaa attgatttaa atcacattaa ccaggattct caatgcaact
     4561 tctaaattaa tccagatcaa taaatggtga attatcatat gtaatgtgat ctatgtagga
     4621 tcatttgtta ctccaatgta ggtatattcg tcacgttttt ataaccataa cgacggagcg
     4681 gatatgaaaa agttaacagt ggcggctttg gcagtaacaa ctcttctttc tggcagtgcc
     4741 tttgcgcatg aagcaggcga attttttatg cgtgcaggtt ctgcaaccgt acgtccaaca
     4801 gaaggtgctg gtagtacgtt aggaagtctg ggtggattca gcgtgaccaa taacactcaa
     4861 ctgggcttga cgtttactta tatggcgacc gacaacattg gtgtggaatt actggcagcg
     4921 acgccgttcc gccataaaat cggcacccgg gcgaccggcg atattgcaac cgttcaccat
     4981 ctgccgccaa cactgatggc gcagtggtat tttggtgatg ccagcagcaa attccgtcct
     5041 tacgttgggg ccggtattaa ctacaccacc ttctttgata atggatttaa cgatcatggc
     5101 aaagaggcgg ggctttccga tctcagtctg aaagattcct ggggagctgc cgggcaggtg
     5161 ggggttgatt atctgattaa ccgtgactgg ttggttaaca tgtcagtgtg gtacatggat
     5221 atcgatacca ccgccaatta taagctaggc ggtgcacagc aacacgatag cgtacgcctc
     5281 gatccgtggg tgtttatgtt ctcagcagga tatcgttttt aattccgcac aaaaacgacc
     5341 ccgtaatata cggggtcaat aaggacatgg tataaagggt attatttctt cgcttctacg
     5401 ccatcagttt cagaacgaat taaaaatttc tcagttgttt gcggaatatg ctgaatcagc
     5461 cagtcggcca tttgcttttc ctcatttaaa atcgcttcga tgatcggaat tgagactgta
     5521 tcaccggcat tttttgctgc tgctaatagt gaggtgtaac aggcgatttc aaattgctcg
     5581 aagacatatc cgctaataga gcctttaact atttcatcag aagggaatat accaccgatt
     5641 gactgcccaa gcgcagccat tttactcatg gaatctttaa tgactgaacg tgaaatgtca
     5701 ttacgatcaa gaatagtttc cagttgaaca atctggtttt tggtttcact aagatgttgt
     5761 tcaatacgag cgcgtagttc aggataatta tctatacggc tggccatgga ttcaagcata
     5821 gattcggctt gcttttccat tgcgtgggcg tcacgtaacc agtcatgata atgttcaata
     5881 cgattcatga aaatgtcctc tttataaaat gactaaaagt taaaattcat atttcaggct
     5941 ttattttcgg ctttcttatt tacgttatta agggccagat cagtcagttt gatgtcggtg
     6001 gccttttctt cttccagggt ttctttcaga agcttcgctg ctttacggta acctaattgt
     6061 tcagccagcg tcgctaatgt cccgtaactg gcaatctcat aatgctcgac tttctgtgct
     6121 gcggcaatca gtgcggcatc acgcacttcg tttttctcgg tactttcgat gacctcatta
     6181 gcttcttcaa taagaccttc cattgccaca catttcatgc gcttaatttt cagatttgat
     6241 tctgattcca caacttgatc aattcgttca atctgtccat gagtttcctc gaggtgcgca
     6301 tgaaaagcct gacttaattt ttcatttgat gttgctcttg cgagttttgc cagtgcccgg
     6361 ggtaattgtt tttctgcgct gtaggtatct gaaagcagtt gaataaatac atcttcaatg
     6421 gtcttcatat tcatattttt ctccagtgaa atcactgcga gccatacggc ccgcagatgc
     6481 aattatcttt atcagctgat attaataatt aacggtgaat caggatttgt ctgatttatt
     6541 accaccgctt tgttgaccgc cttttttacc cgcttcagat gcgcgttgcg gatcattttt
     6601 aaaattaccg ccgctatgct gaccgccttt acggcctgcg tcggatgcct tctcacggtc
     6661 ttcggcgaaa tttcctgaac caccacgatg ttcggccatg ttatttctcc cgttgcgttg
     6721 cattgtttca ttaatatgag tgttgtgtgt cgacactcat taaaattagt cgctaatgag
     6781 aattagtcaa attaagcgca acgagaagat agagggaaaa tatattttga ggaacattct
     6841 ggatatatta acaattacct gaggaataaa tgacttagag aaaattattg attttactgg
     6901 tgttatgttg cgggattaat ttgtttgatt atattactgt tgggcggaaa aatgacgtaa
     6961 gttgacgttc gaccggggta agcgaaacgg taaaaagata aatattaaat gaatttagga
     7021 tttttccggc ttcattaaag aaagttaaaa tgccgccagc ggaactggcg gctgtgggat
     7081 taactgcgcg tcgccgcttt catcggttgt acaaaaactt tcagtgccgc cagcattttc
     7141 tctggctcat taatatgttg ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc
     7201 gcagctcctg catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaacccctgc
     7261 aatggaggtg cagcgttgta ctctttcagc ttcgcaacca gatgattgag gggtaacgcg
     7321 gcgcggtttt ctgcgccggt cacgcccgct cgtgacagca aatatgtgta accacgaccg
     7381 taagaggcta tctggcgcag caggtcgtca tcggcatttg gcgggcagat gaagataggt
     7441 gcgacattat ggcgcaacgc ggcctggcgg aagggcgcgg actcttcaac tggcacatcg
     7501 gcaaccagca ccgaatcgac gccgactttc tcgcactggg cataaaactc atcaatgcct
     7561 ttgttaaaca ccagattggc atacatcaac aggccaatgg gaatggtcgg gtgtttctgg
     7621 cgaatcagtg ccagcatttc aaaacattgt gccggagtca cacctgccgc aaaggcgcgc
     7681 agagtggcgt tttgaatggt cgggccatcc gccagtgggt cggagaaggg gatacctaac
     7741 tccagcgcgt cagcaccggc ttcaattagc gtatcgataa ttttcaatga ctgctcaatg
     7801 cccggatcac cgagcgtgac gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg
     7861 gcaaacagag attcgtagcg ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa
     7921 cggtgaagat gtctttatcg ccgcgaccgg aaaggttaac caccagtagc tgctctttat
     7981 ccgggttttc gcgcatcatt ttcagcgcat gggccagggc gtgggaggat tccagcgccg
     8041 ggatgatccc ttcgtgcagg cacagcgttt tgaaggcttc aagggcttca tcatcggtaa
     8101 tagacacgta atcagcgcgt ccagtgctgt taagatacgc gtgttgtggg ccgacagacg
     8161 ggaaatccag tccggcggag atggagtaag attcttcaat ctgcccgtct tcggtttgca
     8221 tcatcggcgc tttcataccg aaataaatgc ccacgcgacc atgttttagc ggtgcgccgt
     8281 gctcgccagt ttcgataccg tgaccaccag gctccacacc aatcaggccg acgttggttt
     8341 cattgatgaa atcagcaaac atgccgatgg cattcgaacc gccgccaaca caggcgataa
     8401 cggcatccgg caggcgacct tctctttcca gaatctgcgc tttggtttct tcgccaatca
     8461 tccgctgaaa ctcacggaca atggtcggat aaggatgcgg gccagccgcg gtgcccagca
     8521 tatagtgcgc ggtttcataa ctgccggacc agtcgcgtag cgcctcatta caggcatctt
     8581 tcagcgtggc ggaaccgcta tgtaccggga tcacttccgc acccattaag cgcatacgaa
     8641 aaacgttagg cgactggcgt tcaacgtctt tggcacccat ataaatacgg catttcaggc
     8701 cgagcagggc gctggcaagg gccgacgcca cgccatgctg accggccccg gtttcggcga
     8761 tgatttcggt tttacccatc cgcttcgcca gcaacgcctg ccccagcacc tggttggttt
     8821 tatgtgcgcc gccgtgcagc aaatcttcac gcttgagata cagcgtggtg ttcgtcccgg
     8881 ctgtaatgtt ctggcatttg gtcagtgcgg ttggacgccc ggcatagttt ttcagcaggt
     8941 cgttgaactg ggcttgaaat tcaggatctt tttgcgcgct gacaaaagct tcttccagct
     9001 ggcgcagagc aggcatcagg atttgtggca cgtacatgcc gccaaactca ccaaaatagg
     9061 ggttaagtaa tgttgtcatt gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga
     9121 ggccaaaaga cgtgcgtctt tgatgcccgg ttgcgactct acagcagaat taaaatcaag
     9181 tccggcgcag ccggtttgtg ccgcttccac gcagttatct gcgcctaagc cccccgccag
     9241 cagaacgttg ccaagcgatt gaccatttaa tagtgaccag tcaaaacgtt gcccgcttcc
     9301 accctggccg ttgtctaaaa catatttatc aacgtgctga aactcgcggg cgggcagggt
     9361 ttcaccgacg cttaatgctt tccagatggc aacatgtgct ggcagagctt cacgcagcgt
     9421 atcgatatac agctgatctt cattaccatg cagttgcact gccgccagcg ataacacctt
     9481 agctttgtcc accacatcgg caatatcgtg attgcggaac acgccaacat actgcaacgg
     9541 tgccgcagcc atcacttcct gcgcctgttc aacgttgacg caacgcggtg atgtcgcaac
     9601 aaaaatcaac ccaccgtaaa tcgcgcccgc gtcataagct gctttagcat cttgcccacg
     9661 cgtcaggcca catactttat tctcacccag caacacccgg cgcacggcgg cgtgcaaatc
     9721 gtcatgggcc atcaacgccg aaccaatcag aaaaccgtta gcgaagtggc ttaactcgcg
     9781 cacctgagcg taagtattga tgccggattc gctgattacc gttacgttgt gccccagttt
     9841 cggcgcaagc tcgcgggtac ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt
     9901 gatgccaacg acctttgccc ctaatgcaat ggcgcgctcc agttcctctt cattactgac
     9961 ttcggtcagc acccccatct ccagactgtg agcgacggct gcaagctggc gatattggtc
    10021 gtcatccagt actgaaagca ttaataagca ggcatcggcc tggtaatagc gcgccagata
    10081 gatctggtaa gggtcgataa tgaagtcttt acataaaatc ggctgcgggg cgatttggct
    10141 gacgatgagg aggaaatcaa agctcccctg aaaatatttc tcatcagtca gcaccgaaat
    10201 tgccgaagcg taatgtttat aaatggcggc aatgcgtgcc ggatcgaaat catcacggat
    10261 cacgcctttt gacggcgacg cttttttaca ctccagaata aacgccgtgc gtgcaccctg
    10321 tagcgcatca taaaaatgtc gtgtgctcgg ctgaacctca ttctgaaaac tggccagcgg
    10381 ttgctgctgt ttgcgggttt ctacccaaat cgccttgtct gcgacgattt tcgctaaaac
    10441 ggtttgcatc atttaccctc gtgcagccag cgcggtaact ctgtcgtaag cggaaccact
    10501 gcgcagtacc tcaagaacgg tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg
    10561 cattaacatg gcgacgttcg cagcgacggc tgcttcatgg gcggcgtcgc ctttaccttg
    10621 taacaagcgt gttaaaatgt cacggttttc ttcaggtgtt ccgcctgcca gttgctcctg
    10681 gtggtaggga gtcaggccaa aatcttcagc ggtcaattga tagctcttaa ttttgccgtc
    10741 atgcagctcg gcaacgattg tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg
    10801 caccaccgcc gcgcgttgat accccagcac acgcaaggtt tcggcaatcg gcagcaccag
    10861 ttccggacta taaacaccaa ttaacgccag cggcggatgc gccgggttaa tcaatggccc
    10921 cagcacattg aacagggtgc gggttttcag ttgctggcga accggcatcg cgtggcggaa
    10981 tccggtgtga tatttcggcg caaagaggaa acatacgcct aactcgtcca gcgcctggcg
    11041 cgatttattg gcgttcatat caagattaat accgaatgcc gccagcagat ccgacgagcc
    11101 ggatttactg gagacgctgc ggttaccgtg tttcgccact ttcagcccac aggccgcggc
    11161 gacaaacgca ctggcggtag aaatattgat actgttgctg ccgtcaccgc cagtaccgac
    11221 gatatcagca aacagataat ccgggcgcgg gaacggcgct gcgttttcca gtagcgcggt
    11281 tgctgccccg gcgatctcgt tcgggtgctc accgcgaatt ttcatgctca ccagcgccgc
    11341 cgccagttgt tccggcttca gctcgccacg caccaccgct gaaaacagct ggtggctttc
    11401 ttgttggcta agcgtctgcg cctgatacag tttttccaga atcggttgca gcgtgttggc
    11461 tggctctagt ttctgctgcg cccaggccag cgtttgttcc agcaggcgag cgccctgggt
    11521 ggtgagaatg gattccggat ggaactggaa tccacaaacg cgatccgcat cgtgacgtac
    11581 tgccatcacc atgccattaa aatgggcgtt gatggttaaa ccggccggaa tgttactgcc
    11641 aaccagcgag tgataacgcg ccaccggcag cgggtttgtt aatccggcaa acatcgcctg
    11701 accgtcatgt tcaatgctcg acgctttacc gtgaagaatt tcgcccgcct gaccgacata
    11761 gcccccgtaa gcttcgacaa ttgcctgatg tccgaggcaa atgccaataa tgggcagctt
    11821 gccacgcaag cgggtgagga gttccggcat acaaccggct tcgctcggca caccggggcc
    11881 aggagaaagc atcagtaccg gattgctcat cgtcgccagg cgttcaatta aggtctgcgc
    11941 cggaatatgg ttgcggtaaa tcaccacgtt atgaccattg ctgcgcaact gatctgccag
    12001 gttgtacgta aaagagtcga tattatcgag cagcagaatg tcagccatca gaaagtctcc
    12061 tgtgcatgat gcgcggtggc aatagcgcgc agtacagcgc gggctttgtt acgggtttcg
    12121 tcggcttccg actgcggaac agaatcaagg actacaccag cacccgcttg cacggtggcg
    12181 ataccgtttt ccaccagcgc cgagcggatc acaatgcagg tgtcgagatc gccatgcgcg
    12241 gtgaaataac ctaccgcgcc gccgtagctg ccgcggcgac gaccttccgc ctcggcaatt
    12301 aactgcatag cgcgtacttt cggcgcaccg cttaacgtcc ccatattcat acaggcgcga
    12361 taagcgtgca gggcgtcaag atcgtgacgc agttcgccga ctacgcgaga gacgaggtgc
    12421 atcacatagg aataacggtc aactttggtg agatcggcga cgtagcggct gccaggggtg
    12481 caaatgcgtg ccagatcatt acgggcgaga tcaaccagca tcagatgttc agacagctct
    12541 ttatggtcag tacgcatttc cagttcaata cggctgtcga gatctctgtc cagtgaacca
    12601 tcggcgcgac gaccgcgtgg gcgtgttccg gcaatcgggt agatctcaat ctggcggctg
    12661 gtggcatcat acttgagcga gctttccggc gacgcgccaa atagggtgaa atcattatcc
    12721 tgcataaaaa acatgtacgg gctgggatta ctctttttca gcacgtaata ggccgccagc
    12781 ggtgacgggc agggcagaga gaaacggcga gatggcacca cctggaaaat ttctccagcg
    12841 cgaatcgctt tttgcaacaa acgcactacg ccaccgaact cttcatcgct ctgattacat
    12901 tcacaacgca tatgcggcac ggaaaccact ggcagcggcg gcgcggcttc ggtcagttgc
    12961 tgacgtagtt cgttcaggcg agcagtaaga cgttgttttt cttcttcatt cggagcaaac
    13021 aggctggcct gaatacgggt gctttttttc tgatggtcaa tcaccatcag cgtttcagcg
    13081 agataaaaac agaaatcagg gcagttattt tccgctgaca gttgcggtaa atcttcaaat
    13141 cccgccacaa ggtcataaga gaacaggccg ccgaagaaca ttgcttctcg ttcttccttc
    13201 ggtacattca acagattctg caataaacgg aaagcgtcaa aaaccgaaag ggagcataag
    13261 cgggcgtctt catccagcag tggactgaca ggggggaagc gcagcacacg gcagtttggt
    13321 gattgttcac tttccacacc cgcaggcagg gcgttatcca gtagtgccag cagggcttcg
    13381 ccattgccgg aaagtgcctg aattgtgacg gtgtcaccta atgctgtaat gtgcagcgca
    13441 ctgtctatca gcagcaggct ttttaaatca tctttgctgt cgatatctgc ggattccagc
    13501 agcagcgttg ccggacgatt cccacacaac tggtgaaaaa gcgcagtcgg gttgtcgcga
    13561 taagcgcctt cgcaggttag cagttcgaga gtcggttttt gtgtttgcat tgttattctc
    13621 taattttgtt caaaaaaaag cccgctcatt aggcgggctg ggtatctgat tgctttacgc
    13681 atggtgaata cactgcccgt ttcaggaagt ggccaccaac ctttcagtac gaaaattgct
    13741 ttcattgtcg ataccatttt tacgtgaact tgcgtactag tcaactggtt cgatgattaa
    13801 ttgtcaacag ctcatttcag aatatttgcc agacccgtta tgatgtcggc gcaaaaaaca
    13861 ttatccagaa cgggagtgcg ccttgagcga cacgaattat gcagtgattt acgacctgca
    13921 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
    13981 cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg caatcgcgcc
    14041 agcaagagag gaaatttcac gttcgggact ggcgctgaat cttattcccg gcgtggaaat
    14101 ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca
    14161 tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagca
    14221 aattgccgaa agacttgaaa aagcgcaaat acctggggcg ctggaaggcg cacaacgact
    14281 ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt ttccttgtgg agtgcggcaa
    14341 agccagttca atggcagatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt
    14401 tccgccacag tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa
    14461 ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt
    14521 tgcgcatttt gccgaacacc acggcgacgc gatggaagtc gcgcagtgcc agcaatcgcc
    14581 caatgaacgc acccagctgg ccacacttgc gcgtcagcat catttatggg catcacaagg
    14641 atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc
    14701 aggcgttgaa ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt
    14761 atgagccagt ttttttatat tcatcctgat aacccgcagc aacgtctgat caaccaggcg
    14821 gtggagatcg tgcgtaaagg cggggtgatt gtctatccaa ctgattccgg ctatgcgctc
    14881 ggctgtaaaa gtgaagataa aaacgccatg gagcgtattt gtcgtattcg ccagctgccg
    14941 gacggtcaca actttaccct gatgtgtcgc gatctttctg aactctcgac ctattcattc
    15001 gtggataacg ttgcgtttcg tttgatgaaa aacaacacgc cgggcaacta taccttcatc
    15061 ctgaagggga cgaaggaagt gccacgccgt ctgttgcagg agaaacgcaa aaccatcggt
    15121 atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg aagcactggg cgaaccgatg
    15181 ctttccactt cgctaatgct gccaggcagc gaatttaccg aatccgatcc cgaagaaatt
    15241 aaagaccgtc tggaaaaaca ggtagatttg attattcacg gcggctatct cgggcagaaa
    15301 ccaacaacgg ttattgatct taccgatgat acgccggtcg tggtgcgtga aggcgtaggt
    15361 gatgtgaagc ctttcttata agtgcgtaac tctgtatact acgcggcctt aaaatggcgc
    15421 tatacggcgc cattgattct cctgttcgac gcctgtgaag gcgacaccaa aaggaagctc
    15481 tatgagcgaa aaattacaga aagtgctggc ccgcgctggc cacggctccc gccgtgaaat
    15541 cgaatctatt attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga
    15601 tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat ggtcatctga tttcggtacg
    15661 tgagtccgct gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg
    15721 tacccgtaac gatccggaag ggcgtccgac ggtgtttgac cgtctgccaa agctgcgtgg
    15781 cgcacgctgg attgccgtag gtcgtctgga cgttaacacc tgtggtctgc tgctgttcac
    15841 caccgatggt gaactggcaa accgtttaat gcatccaagc cgtgaagttg aacgtgaata
    15901 cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt
    15961 gcaactggaa gatggtccgg cagcttttaa aaccatcaag ttcagcggcg gcgaagggat
    16021 caaccagtgg tacaacgtga ctctgaccga aggccgtaac cgtgaagttc gtcgtctgtg
    16081 ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt cgttacggtg atatcccact
    16141 gccaaaaggt ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct
    16201 gcgcgaactg gtggagcttc cgccagaaac cagctctaaa gtcgctgtag aaaaagaccg
    16261 tcgtcgcatg aaggctaatc agattcgtcg tgcggtgaaa cgtcacagtc aggtgagcgg
    16321 cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag tatgccggac gtcatatccg
    16381 gcatttttac agattaataa tcaatcccta tttgcgcctt aacaccggca tcaaacgcat
    16441 gtctgatggg gcgcagctca cttaccgtat ccgccagttc aagaatatcc cgatgacagc
    16501 ctcgacccgt gataatcacc gtctgttgat gtggacgttc atttaacgcc tgcaccactt
    16561 cttccagtgg caaatagtca tacgccacca tatacgtcag ttcatcaagc aaaaccatat
    16621 ccagtgagga atcagcaagc atccgctttg catgttgcca gacttcgcgg caggcggcgg
    16681 tatcagactc gcggttttgt gtatcccagg taaagcccgt tgccatcacc tgaaactcaa
    16741 cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca ggtgccttta ataaactgca
    16801 cgacgcccac tttttttccg tgaccaactg cgcgtgttgc cgtaccaaat gccgcggtgg
    16861 ttttgccttt tccattgccg gtaaagacga tgataatacc gcgttcatcc tgggcctggg
    16921 ccacacgagc atctactttt tctttcactc gctgctgacg ctgttggtag cgttcatcac
    16981 tcattgggaa attcctggtt tacggcccgg ttgggcgtca aaggtcatgc cggttttacg
    17041 gcggctgtca tcgcccatca gccagaggta gagcggcatg atatcagcgg gtgttttaag
    17101 tttctgtgga tcttcggtcg ggaaggcgct tgcgcgcatt gcggtgcgcg taccgcctgg
    17161 gttaatgcag ttgacgcgca ggcgctgctg atattcatcg gccagtacct gcatcatccc
    17221 ttcggtggca aatttcgacg ctgcatatgc accccagttg gctcgtccct gacgtccaac
    17281 gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg agtaataaag gaagcagtgc
    17341 ctgggtgagc ataaaggtgg cattaacgtt gacctgcatg acgtcctgcc agacctgcgg
    17401 attttgttcg ctcattgggc aaacatcgcc gagcaatccg gcattatgca aaacaccatc
    17461 cagacgcgga taattaacga caatgcgctg tgccagttgt tggcaatttt cggacgtgca
    17521 ggtcagcaaa tcgagaataa accactgtgg ctgacgccca gtttcttcgt ttatgtggct
    17581 ggctacctga cgtaattttt cttcattacg gcccaacaga atcactgtcg caccatagcg
    17641 tgcatacgtc atcgcggctt cacgaccaat cccatcgctg gctcccgtca ccaggataat
    17701 gcgatcattg agtaaatctt gttttggctg gtaatgcatg gctactcctc aacgacgttg
    17761 tctgtcgcgc tgccagtgta ctttatgact ttggggcttt atgcctgaaa cagaagggca
    17821 tttcaatcag cctggggcga taacaccgca aaatggatac tttgtcatac tttcgctgca
    17881 ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa ctgtgagcca aagcgttgtt
    17941 taaccaaggt ggggactcgt ggaattgttg tctgaatatg gtttgttttt ggctaaaatc
    18001 gttaccgttg tgctagcgat tgcggcgatt gccgccatta ttgtcaatgt tgctcaacgt
    18061 aataaacgcc agcgtggcga gttacgggtc aacaatctca gcgaacagta taaggagatg
    18121 aaagaagaac tggccgcggc gctgatggac tcacatcagc aaaaacagtg gcacaaagcg
    18181 cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag cgaaagccaa actgggcgag
    18241 gtggcaactg acagtaaacc tcgcgcctgg gtgctggatt ttaaaggcag catggacgcc
    18301 catgaagtga actcgctacg tgaagagata acggcggtac tcgcagcatt caaaccgcag
    18361 gatcaggttg tgctccgtct ggaaagccct ggtggcatgg tgcatggtta cgggttggcg
    18421 gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt taactgttac ggtagacaaa
    18481 gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg acaaaattgt ttccgcaccg
    18541 tttgctattg tgggttccat tggggtggtg gcgcaaatgc ccaactttaa ccgcttcctg
    18601 aaaagcaaag atattgatat cgaactgcac accgccgggc agtataagcg cacgctgacc
    18661 ttgctgggtg aaaataccga agaagggcgg gagaaattcc gcgaagagct gaacgaaacg
    18721 catcagttat ttaaagattt tgtgaagcgt atgcgtccgt ctctggatat tgaacaggtg
    18781 gcaacgggtg aacactggta cggacaacag gcggtagaga aaggcctggt tgatgaaatc
    18841 aacaccagtg atgaagttat tcttagcctg atggaaggcc gtgaagtggt taatgtacgc
    18901 tatatgcagc gtaaacgact cattgaccga ttcaccggca gcgcggcaga gagcgccgat
    18961 cgattgttgc tacgctggtg gcagcggggt caaaagccat tgatgtaaaa gacaaacgcg
    19021 aggctaagac ctcgcgtttt gctttaatca accagatgat atttttctga aagcacatgg
    19081 gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg ctgttggcaa tccttgttca
    19141 tctaaaaagt agtcgccggt aaataccagc acgccattac gctgcgtgac gccggtggct
    19201 tcaatccctg cgagcgtatc ctcatgctca cgaatgattt tgttagcctc tttcaacagc
    19261 gtttcgcggt cgatgggttg tgtctctttg ttcattattt actccttaaa caaggacatt
    19321 agtctacgcc aggcatggct tgcagacaaa tataccacgc tggtggcaag agcgccttac
    19381 tggcaacttt ggattttgca tgctaataaa gttgcgtatc ggattttatc aggtacagtg
    19441 tgacgctttc gtcaatctgg caatagattt gcttgacatt cgaccaaaat tccgtcgtgc
    19501 tatagcgcct gtaggccaag acctgttaac tcagtcacct gaattttcgt gaacagagtc
    19561 acgacaaggg ggtgatatcc gcagagagcg agtccatatc ggtaactcgt tgccagtgga
    19621 aggtttatca acgtgcgacg cattcctgga agaatcaaat taggtaaggt gaatatgggt
    19681 aaagctcttg tcatcgttga gtccccggca aaagccaaaa cgatcaacaa gtatctgggt
    19741 agtgactacg tggtgaaatc cagcgtcggt cacatccgcg atttgccgac cagtggctca
    19801 gctgccaaaa agagtgccga ctctacctcc accaagacgg ctaaaaagcc taaaaaggat
    19861 gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt ggcacaattg ggaggcgcat
    19921 tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac tgaaacaact ggctgaaaaa
    19981 gccgaccaca tctatctcgc aaccgacctt gaccgcgaag gggaagccat tgcatggcac
    20041 ctgcgggaag tgattggtgg tgatgatgcg cgctatagcc gagtggtgtt taacgaaatt
    20101 actaaaaacg cgatccgcca ggcatttaac aaaccgggtg agctgaatat tgatcgtgtt
    20161 aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg ggtatatggt ttcgccgctg
    20221 ctgtggaaaa agatcgctcg tggtctgtct gccggtcgtg tgcagtcggt ggcggtccgc
    20281 ctggtggtag agcgtgagcg tgaaattaaa gcgttcgtac cggaagagtt ctgggaagtc
    20341 gatgccagca cgactacgcc atctggtgaa gcgttggcgt tgcaggtgac tcatcagaat
    20401 gacaaaccgt tccgtccggt caacaaagaa caaactcagg ctgcggtaag tctgctggaa
    20461 aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga caaccagtaa acctggcgct
    20521 ccttttatta cctctacgct gcaacaagct gccagcaccc gtcttggttt tggcgtgaaa
    20581 aaaaccatga tgatggcgca gcgtttgtat gaagcaggct atatcactta catgcgtacc
    20641 gactccacta acctgagtca ggacgcggta aatatggttc gcggttatat cagcgataat
    20701 tttggtaaga aatatctgcc ggaaagtccg aatcagtacg ccagcaaaga aaactcacag
    20761 gaagcgcacg aagcgattcg tccttctgac gtcaatgtga tggcggaatc gctgaaggat
    20821 atggaagcag atgcgcagaa actgtaccag ctaatctggc gtcagttcgt tgcctgccag
    20881 atgaccccag cgaaatatga ctccacgacg ctgaccgttg gtgcgggcga tttccgcctg
    20941 aaagcacgcg gtcgtatttt gcgctttgat ggctggacaa aagtgatgcc tgcactgcgt
    21001 aaaggcgatg aagatcgtat cttaccggca gtcgataaag gcgatgctct gacgctcgtt
    21061 gaactaacac cagcccagca ctttaccaag ccgccagccc gtttcagtga agcatcgctg
    21121 gttaaagaac tggaaaaacg tggtatcggt cgtccgtcta cctatgcgtc gatcatttcg
    21181 accattcagg atcgtggcta tgtgcgagta gaaaatcgtc gtttctatgc ggaaaaaatg
    21241 ggcgaaatcg tcatcgatcg ccttgaagaa aatttccgcg agttaatgaa ctacgacttt
    21301 accgcgcaga tggaaaacag cctcgaccag gtggcaaatc acgaagcaga gtggaaagct
    21361 gtactggatc acttcttctc ggatttcacc cagcagttag ataaagctga aaaagatccg
    21421 gaagagggtg gtatgcgccc gaaccagatg gttctgacca gcattgactg cccgacttgt
    21481 ggtcgcaaaa tggggattcg cacagcgagc accggggtat tccttggctg ttctggctat
    21541 gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc tggtgccgga aaacgaagtg
    21601 ctgaacgtgc tggaaggcga agatgctgaa accaacgcgc tgcgcgcaaa acgtcgttgc
    21661 ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc cgaaacgtaa gttgcatgtc
    21721 tgtggtaata acccaacctg cgacggttac gagatcgaag agggcgaatt ccgcattaaa
    21781 ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct ctgaaatgca cctgaaaatg
    21841 gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt gtaaaaacac acgtaagatt
    21901 ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg tgccattacc tgagctgccg
    21961 tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg ctgccggtgt gttcctggct
    22021 gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga gctttatcgc
    22081 ttccgcgacc gtctgccgga aaaactgcgt tatctggccg atgcgccaca gcaggatccg
    22141 gaaggtaata agaccatggt tcgctttagc cgtaaaacca aacagcaata tgtctcttcg
    22201 gaaaaagacg gaaaggcgac tggctggtca gcattttatg ttgatggcaa atgggttgaa
    22261 ggaaaaaaat aacctttaat tctgtcaggt ttttataaac aaagggtcgc gaaagcggcc
    22321 cttttttatt gcatattatt ttttcttcac acctatacac taaggctata aatgatatag
    22381 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
    22441 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
    22501 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
    22561 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
    22621 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
    22681 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc
    22741 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
    22801 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
    22861 accagggccc gccgacacaa attgctgatg ccgtctctaa aggcaatgcc gatttcgcta
    22921 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
    22981 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
    23041 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
    23101 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgttttcacg gcaacggatg
    23161 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
    23221 cggtggatcc ggtcgccgat cccgaccttg tgcgcgttga tgctcacgat atcttcagcc
    23281 acagtacaac caaaattggt tttcgccgta gtactttttt gcgcagttat atgtatgatt
    23341 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
    23401 gctccaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
    23461 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta
    23521 cttatttcta taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt
    23581 aaagtagtgt ctaattaccc gcctcgcaaa ttccagtttt caaatatcta tttcaggtca
    23641 aaagattgaa ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt
    23701 ctttaggatt tgtctcatcg tatgattaat tacaccaatc attcaacgtc ttgctgataa
    23761 gtgatggcga ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgtgatc
    23821 ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg
    23881 ttgtcgcact gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga
    23941 gtcgtcacca ttatgccagc atagtgacaa tgaaccagaa acgaaacggg aatgttccgt
    24001 cgttattcca gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc
    24061 tgaagatgat cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt
    24121 aaagggcttt taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa
    24181 ccgttaaggg tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg
    24241 ttttctctgt cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg
    24301 accccgctgc ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt
    24361 tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag
    24421 aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc
    24481 cagtaaggac acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc
    24541 tgctaaatca ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa
    24601 cctgctgcgc tggcaggatg gtaactcagt taccgaagag gatatccacg cactggcggg
    24661 atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat
    24721 gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa
    24781 acgcctcggt ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga
    24841 ccactcggtg accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct
    24901 ggaaatggaa cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag
    24961 tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct
    25021 cggcaaagca gtgtggagcg aattgcagga cggtgaatgg attgcttatc cggatacact
    25081 cgttggta
//
LOCUS       DADLZM010000049        24861 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLZM010000049 DADLZM010000000
VERSION     DADLZM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.049"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..352
                     /locus_tag="JNY73_001947"
     CDS             71..352
                     /locus_tag="JNY73_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY8047975.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1394
                     /locus_tag="JNY73_001948"
     CDS             318..1394
                     /locus_tag="JNY73_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8047976.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMADWIERYKTILIRRKASRNTYKTRVN
                     QLVTIKENLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGILFSENPPTFHEIRSLAGRLYKEICGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1787..2128)
                     /locus_tag="JNY73_001949"
     CDS             complement(1787..2128)
                     /locus_tag="JNY73_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY8047977.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2141..3013)
                     /gene="copD"
                     /locus_tag="JNY73_001950"
                     /pseudo
     CDS             complement(2141..3013)
                     /gene="copD"
                     /locus_tag="JNY73_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="JNY73_001951"
     CDS             complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="JNY73_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY8047978.1"
                     /translation="MASTARSLRYALTILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3530..3760
                     /locus_tag="JNY73_001952"
     CDS             3530..3760
                     /locus_tag="JNY73_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY8047979.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3862..4518
                     /locus_tag="JNY73_001953"
     CDS             3862..4518
                     /locus_tag="JNY73_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY8047980.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4542..5204
                     /gene="exoX"
                     /locus_tag="JNY73_001954"
     CDS             4542..5204
                     /gene="exoX"
                     /locus_tag="JNY73_001954"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY8047981.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="JNY73_001955"
     CDS             complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="JNY73_001955"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY8047982.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQHWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7470..8129)
                     /locus_tag="JNY73_001956"
     CDS             complement(7470..8129)
                     /locus_tag="JNY73_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY8047983.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8456..8812)
                     /gene="yebF"
                     /locus_tag="JNY73_001957"
     CDS             complement(8456..8812)
                     /gene="yebF"
                     /locus_tag="JNY73_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY8047984.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8879..9169)
                     /gene="yebG"
                     /locus_tag="JNY73_001958"
                     /pseudo
     CDS             complement(8879..9169)
                     /gene="yebG"
                     /locus_tag="JNY73_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9303..10481
                     /gene="purT"
                     /locus_tag="JNY73_001959"
     CDS             9303..10481
                     /gene="purT"
                     /locus_tag="JNY73_001959"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY8047985.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10536..11177)
                     /locus_tag="JNY73_001960"
     CDS             complement(10536..11177)
                     /locus_tag="JNY73_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY8047986.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGVGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11214..13025)
                     /gene="edd"
                     /locus_tag="JNY73_001961"
     CDS             complement(11214..13025)
                     /gene="edd"
                     /locus_tag="JNY73_001961"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY8047987.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13260..14735)
                     /gene="zwf"
                     /locus_tag="JNY73_001962"
     CDS             complement(13260..14735)
                     /gene="zwf"
                     /locus_tag="JNY73_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY8047988.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15074..15943
                     /locus_tag="JNY73_001963"
     CDS             15074..15943
                     /locus_tag="JNY73_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY8047989.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16071..17513
                     /gene="pyk"
                     /locus_tag="JNY73_001964"
     CDS             16071..17513
                     /gene="pyk"
                     /locus_tag="JNY73_001964"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY8047990.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17644..18615)
                     /gene="lpxM"
                     /locus_tag="JNY73_001965"
                     /gene_synonym="msbB"
     CDS             complement(17644..18615)
                     /gene="lpxM"
                     /locus_tag="JNY73_001965"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8047991.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18735..20057)
                     /gene="mepM"
                     /locus_tag="JNY73_001966"
     CDS             complement(18735..20057)
                     /gene="mepM"
                     /locus_tag="JNY73_001966"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY8047992.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20073..21005)
                     /gene="znuA"
                     /locus_tag="JNY73_001967"
     CDS             complement(20073..21005)
                     /gene="znuA"
                     /locus_tag="JNY73_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY8047993.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21084..21839
                     /gene="znuC"
                     /locus_tag="JNY73_001968"
     CDS             21084..21839
                     /gene="znuC"
                     /locus_tag="JNY73_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY8047994.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21836..22621
                     /gene="znuB"
                     /locus_tag="JNY73_001969"
     CDS             21836..22621
                     /gene="znuB"
                     /locus_tag="JNY73_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY8047995.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22768..23778)
                     /gene="ruvB"
                     /locus_tag="JNY73_001970"
     CDS             complement(22768..23778)
                     /gene="ruvB"
                     /locus_tag="JNY73_001970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY8047996.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23787..24398)
                     /gene="ruvA"
                     /locus_tag="JNY73_001971"
     CDS             complement(23787..24398)
                     /gene="ruvA"
                     /locus_tag="JNY73_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY8047997.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24537..24602)
                     /locus_tag="JNY73_001972"
     CDS             complement(24537..24602)
                     /locus_tag="JNY73_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047998.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24673..>24861
                     /locus_tag="JNY73_001973"
     CDS             24673..>24861
                     /locus_tag="JNY73_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8047999.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITT"
BASE COUNT         6165 a         6295 c         6108 g         6293 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcaga ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg tgacaataaa agaaaattta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtgg attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcctgt tcagtgaaaa cccaccaact tttcacgaaa
     1261 tcagaagcct ggctggacga ttgtacaaag aaatatgtgg agaagaattt gctcagcgtc
     1321 tacttggcca cacatcggag aagacaacaa aaatgtatct tgatgagaga gaaaaaacgt
     1381 acttactgct ctgattttaa cgtaaatgga ttgttaaatg tattttggtt gtgatataac
     1441 caaaaaagac cggaatacag aaattcgagt aaatttcggg gaatttcggg gagacgtttg
     1501 caactaattg attttaaata caattaaaaa aagaccgaat acgattcctg tattcggtcc
     1561 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
     1621 tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
     1681 caataaaaag cgcggtggtc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga
     1741 actgaggcgt tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt
     1801 cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa
     1861 tctgaatcac cgaaatgggt tgggcattgg tcttcccact ggcaacttcc ttttgtgcga
     1921 tatcgtttaa aggatactgc acgagggtac ttgggttgat gacatacaaa gcattacccg
     1981 gtcggcaagt cagcattacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa
     2041 aacgactcac ggttatcacc tgtggagccg ccagcgccgc tgcagaactg gtgagtaaca
     2101 gaaacgccag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg
     2161 caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc
     2221 acacaaacca gggtgccgca cgatcttcat cctgcctcat gcgcggtaca agaacataac
     2281 gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt
     2341 ggccctagta agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc
     2401 cgcttgccag tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct
     2461 gaatagcctg gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta
     2521 atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt
     2581 tcgctgttac cccttcattc agcgtcgcat gccccactcc cgccagcaga ataaattgcg
     2641 cggtagtgag cataaacaac aaacgtggca tattccgcgg ttgcataagg gcgacgatca
     2701 acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa
     2761 cagcctgcca aatgtttggc gaaaatacat ctgtccatcc cgttcccatc aacccgccct
     2821 gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac
     2881 gcagagaacg cttcgtcagc aagcgacgaa tcattaaggg ggccagccag gcaccataca
     2941 ttgcaaagcc aaagaccagc atcaacgagg tgaaatggat aaatcgcagc gcgatccagg
     3001 taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt
     3061 ccaccgaaac aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg
     3121 gaacaatcag ttgcttttgg tcctgctcat tccgcttcgc tggtaatgtt ttaatatttt
     3181 cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttcaacgcct tccgagaagt
     3241 ttaaagtgat tgcctgcggg gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg
     3301 ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgtcagcg
     3361 cgtagcgaag ggagcgtgca gttgaagcca tattatctat tcctttttgt aataactttt
     3421 ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc
     3481 caggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa
     3541 tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg
     3601 ggtggctttt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca
     3661 gccagaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt
     3721 caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact
     3781 ttcttacata acgcctgcta aattatgagt attttctaaa ccgcccccat aatttgcagt
     3841 cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct
     3901 cgtaaaacct cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc
     3961 aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg
     4021 cgacgtgccc ttcctgcccc acccgatctt tcactgttag acccgctttg ttatgccgca
     4081 acgacctggc gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt
     4141 cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggaactacca tcagagtcat
     4201 ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accgcaaggt
     4261 atggatatgg acccgacctg ttcactgttt acgacagggc aatgtctggg agaacccgac
     4321 ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat tgccgtgctg
     4381 atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc
     4441 gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat
     4501 atcattccat tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata
     4561 cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg
     4621 gaaaaatcgt caaccccatg agcctcctgg tgcgccccga tcgtcctatt agtccacaag
     4681 cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg
     4741 tgatcccaca ctattacggt agtgaatggt atgtcgcgca caacgccagc tttgaccgcc
     4801 gcgtactgcc tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt
     4861 ggccagggct caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga
     4921 caccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc
     4981 ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac
     5041 gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg
     5101 ttgccgaacg cgatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc
     5161 cggagctgcg tttaacactg aaacattatc tggaaaatac ttaatccgca ggcgtagtgg
     5221 gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg
     5281 atttaaagcg gccagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa
     5341 gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatgtt
     5401 gcacctgaga atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga
     5461 cgttgtcata cgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcggatttc
     5521 cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa
     5581 catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg
     5641 caacgcccat caacatgccc cccgcacttc cgcccatcgc ataacagagc gaaggagagc
     5701 catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct
     5761 tcttcagaaa tttaccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat
     5821 ggacaatggc gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg
     5881 catcaatact tgcgccgtaa gaaccatagc catacaccag caacgggttg tgtcctttgc
     5941 gaaaatgttt gcgatgatag accagcgaaa ccggaacttc gacgccatca cgggcgacta
     6001 tccacaggtg ttcactgcgg taattcgccg catcaaaacc gggcacttct gtttgtttta
     6061 atacacgacg ctcaccggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg
     6121 aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca
     6181 cataggccgg atcatcaaag gcaatgccaa tgacttcccg ggtcttgcgg ttaatttggc
     6241 gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa
     6301 acccttccag catgatgttt tcacgtggcg gaattaactc ttcccactgt tgctcatcac
     6361 gcatacgggt acggtataag ccaaagtttt tgccgtggcg attggaacgc agataaaaac
     6421 gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg
     6481 gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca
     6541 gatgaatgac gacatagtgc ttcgacgtcg ttttatgcag gctgacataa taggtatcgt
     6601 ctttttcttc gtagatcagt gtatcttgcg atgctggcgt accgatggcg tgacgccaga
     6661 cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat
     6721 ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttacctgttt
     6781 ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca
     6841 taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg
     6901 cgcgcttgtt ggcatcgagc aatgtttccc actcatccca ctcttcgctg aatgccgatt
     6961 gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat
     7021 tttttatgta gggcgcagaa acttctcttt gcggaatgcg gtcgatgatt tcctttaaga
     7081 tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt
     7141 gcaggtagtc caggacttct ggctgagaac gcgtatcgtc ccgcagccag tagtaattat
     7201 cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca
     7261 tgttattgtt ctttctggtt gaaacatctt ataagggtgg caaaactcac cggggatgca
     7321 agcgaaacag ggtcgtcatt gcttagatga tgacaggtaa tggcgcggat atcgaatgtt
     7381 atgcagacag agaaaatcag cctgttcaaa tggctgtgcg gttctggata gcccgaaata
     7441 gtcaacttca ggctatccag agagcggaat tattccgcca aagtgcgttt ttgctgttcg
     7501 agatcgcgtt caatgccttc acgaacatcc tgggggattt tcagcgcgtc acccagtgca
     7561 ttcaggtaac tgcgttccat aaaatggtca atatcaatag ccgcgcagct caggaaatag
     7621 atttccagcg cctcttcttc attgcggacc ccggtagcca ggcgttgtgg atccagcggt
     7681 tgttcgattg cctgctcaat gagtacacgc ccctgctctt ccacgccggc ttcacgcaat
     7741 tgctggtcga ttgccgcacg ttcattggca tcaatatgac catcactttt agcggcaaag
     7801 accagcgcaa ggatcaaacg ttccgtacgc tcatccagcg gcgtactttg cgcgccaaac
     7861 tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt atttattcca cagcaccgta
     7921 cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc catatttcgt aagaagttta
     7981 cgtgctgatt tatttgcgac cagcagccca gccagtccgc ctaatgcgcc tggcactaac
     8041 agtttgccca atccctgatc cgcagacgag gaggtagaag aactgctttg cccaagcagg
     8101 gattgcagtt gatttaacca gttagccata tttgctcctc aataaccatt tttagtgcct
     8161 gtagcatagc ggatgaggat gtcagaaaat gtatatcgag acaaaagatg cgcaaataag
     8221 cggggcggta ggccgttatt caaagaaatt cgcgccaggt aaggtattta ctgtatatca
     8281 ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct
     8341 gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg cgttctggga
     8401 cggacaaggc gctcgcgccg catccgccaa cggtcaatcg aattatcaga agccattaac
     8461 gccgctgata ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg
     8521 cacttttacc acgcacggtt agcggtactg accatttatc atctttaccc tgaatgtcct
     8581 gcaaactgac ccaggccacg ggatcggcct gaccgacaat tttttgatca tctgcccagc
     8641 gcgccacgcg attttgttga taatcacgtt ttacgctcgc ggcaattccg gtagcatcca
     8701 gaccttcaca ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg
     8761 atgcgcaggc agaaaccaac aaccgcccta aaaacgcccc tctttttttc atgtttttct
     8821 ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc
     8881 aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgaccagt ggggacggta
     8941 atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa
     9001 gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagctag gtgtcaagca
     9061 gatccgccgt atcaagcatt ttgtcataag catcggcttc ctttttgctg gtaaacgaca
     9121 ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct
     9181 cataataact gtgattttat acagtatatt tcttttcggt tgagaaatca acatcagcaa
     9241 taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg
     9301 ttatgacgtt attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct
     9361 ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg
     9421 ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta
     9481 atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg
     9541 tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga
     9601 atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc
     9661 tggcggcaga agagctgcag ctgcccactt ccacttatcg ttttgccgat agcaaaagcc
     9721 ttttccgcga ggcggttgcc gccattggct atccctgcat tgtaaaaccg gtgatgagct
     9781 cttccggcaa ggggcagacg tttattcgct ctgcagagca acttgctcag gcatgggaat
     9841 acgctcagca aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt
     9901 ttgacttcga aattaccctg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac
     9961 cagtaggtca tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga
    10021 gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg
    10081 gttatggtct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg
    10141 tctcccctcg tccacatgat accgggatgg tgacgttaat ttctcaagat ctctcagagt
    10201 ttgccctgca tgtacgtgcc ttcctcggac ttcctgttgg cgggatccgt cagtatggtc
    10261 ctgcagcttc tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg
    10321 tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg
    10381 gcagccgtcg tctgggggtg gtactggcta cttcagagag tgttgttgac gccattgaac
    10441 gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaacgctac
    10501 aaaaatgccc gatcctagat cgggcatttt gacttttaca gcttagcgcc ttctacagct
    10561 tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc
    10621 agccaggaac caccgatgca cagcacgctt ttcagtgcca ggtagtcacg gtagttagcc
    10681 ggagaaatac cacccgtcgg gcagaaacgg acctgggaga acggacccgc gatcgcctgc
    10741 agggctttca cgccgccgtt agcttcagcc gggaagaatt tgaactcttt caaaccgtag
    10801 tccataccca gcatcagttc ggaaacagtg ctgatccccg gaatcagagg aatagtccct
    10861 tcggtagcag ctttcagcag cggctcggtc agacccgggc taattgcgaa ctgtgcaccc
    10921 gcttcagtga cttctgccag ctgctgtgga ttcagcaccg taccgacacc cacaatcgct
    10981 tcaggcactt ctttggcgat agcacggata gcgtcaactg cacactcggt acgcagagtc
    11041 acttccagaa cgcgcacccc accagcaacc aacgcttttg ccatcggcac cgcgtgttcc
    11101 agttttttta ccacgataac cggtacaacc gggccggtgg tcaggattga ttctgcactt
    11161 gttttccagt ttttcatcag agttttctct cgcctgatta caaatttgtc gtcttaaaaa
    11221 gtgatacagg ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc gctgaataat
    11281 tcacgtcctg ttctcacgcg tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc
    11341 agttccgctt cgtctaccag cagtgtcagt tcgcctgtct gtccattcac acgaatgatg
    11401 tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg cttctggtgt tacgtggata
    11461 gctgacggca ctttacctga agcgccggag agtcgtccat cggtaactaa cgcaattttg
    11521 aaacaccggt ccaataatac accaagtggc ggcatgagtt tatgtaattc tggcattccg
    11581 ttcgcttttg gcccctgatg acggacaaca acgacacaat cgcggtccag caaacccgct
    11641 tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc
    11701 tggttctcaa ccggcacggc agaggttttc ataaccgcac ggcccaggtt accgcttaac
    11761 acttttgtcc caccatgatg agagaaaggt tgttcgaagg aagcgatcac attgctgtcg
    11821 agtgattttt ccgccccttc ccgccagtcc agttcaccat tattcagcca tggttcaagg
    11881 gtataacgaa acagaccaaa gcctgccacc gtattgacat cttcatgcag caggcctgct
    11941 ttgagcagtt cacgcaccag aaccggtacg ccacctgccg cctggaagtg gttaatatcg
    12001 gccggaccgt tcgggtagag acgtgccatc agcggtacaa catcagaaag gtcagagaag
    12061 tcatcccagt taatctgaat accggccgcg cgcgccatcg ccaccaggtg catggtgtgg
    12121 ttagtggaac caccggtcgc caacagtgca acgataccgt tcaccaccac tttctcatcg
    12181 atcatcttac cgatcggcat ccattcatta ccattaccgg tcatgcgtgt aacctgacgc
    12241 gcagcggcag cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa agaagagcct
    12301 ggcaattgca tccccataaa ttccaccacc atctggttgg tgttggcagt accgtagaaa
    12361 gtacatgttc ccggcgcatg gtaagacgcg gcttctgact ccagtaaggc catgcggtcc
    12421 actttacctt cggcataaag ctggcgaata cgcacttttt ctttatttgg caaaccgctt
    12481 gccatcggtc cagacggcac aaacaccgca ggcaaatgac caaacgacag ggctgccatc
    12541 gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta
    12601 tgggacagcc ccaccgccgc agacatcgct atcacttcgc ggcttagcag cgacaattcc
    12661 attccatcct gcccctgggt gacaccatca cacatcgccg gaacaccgcc cgcaacctga
    12721 ccaaccgcat tcgcttcatg cagggcttta cgaatgattt ctggatagtg ttcataaggc
    12781 tggtgcgcgg agagcatgtc gttataggag gtgatgatgg cgatattgtt acgcaacatg
    12841 cttttcaaag aggctttgtc ttctggctgg caggcagcga aaccgtgtgc caggttaccg
    12901 catgccaact gcgaacgatg aacggtcgac gttttcgctt gttctatccg ggcgagataa
    12961 gcagagcgag tctcgcgcga acgttcaatg attcgatttg ttacgcgtaa caattgtgga
    13021 ttcataaagg ctcctgaaat tgagttgtca gagcaggatg attcacaacg cgtttcattc
    13081 agaggattta tgagtgaaac gcctgtaacc ggagctcata gggcaaaaac gtttcagtca
    13141 gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt
    13201 ctacaatctg tgcaagatca tgttaccggt aaaataacca taaaggataa gcgctgatat
    13261 tactcaaact cattccagga acgaccatca cgggtaatca tcgccaccga ggcaacgggt
    13321 ccccaggttc cggcctgata cggtttcggc gcatcattgt ccatcgccca cgcctcagta
    13381 atggagtcta cccatttcca ggcttcttcc acttcgtcgc gacgtacaaa cagtgcctga
    13441 ataccacgca tggtttccag cagcaaacgt tcataggcat ccgccagatg cgtctgatta
    13501 aaggtttctg aatagctcag atccagcttg gtgatttgca ggttatgttt gtggtcaagg
    13561 ccaggaactt tattcagtac ctggatatcc acgccttcat caggttgcag acggatagtc
    13621 agtttattct gcggcagatc ctgccacgac tctttaaaca gattcagttc aggtgttttg
    13681 aaatagacca cgacttcaga acatttggtc ggcagacgtt taccagtacg caggtagaat
    13741 ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta
    13801 ttgctgcttt tgttcgcgcc ctcttcttcc agatatcccg gcactttttt gccctgggcg
    13861 aagcccgcag tatattgccc gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg
    13921 cgcagggact tcagtacttt cactttttca tcgcggatgc tgtctgcgct caggtcagat
    13981 ggcggagaca tcgcaatcat gcagagaatt tgtagcaggt ggttctgaat catgtcacgc
    14041 atctgaccgg ctttatcaaa atagccccag cgcccttcga tccccacttc ttctgccacg
    14101 gtaatctcaa catgatcaat ggtgcgattg tcccagttat tcacaaacag ggagttagca
    14161 aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa gatagtggtc gatacggtaa
    14221 acctggcact cctcgaagta ttcgccaacc tgatcattga tttcctgcga ggtcgccagc
    14281 gacgtcccca gcggtttctc catgactacg cgtgccggtt tagcattcag ttttgcctcg
    14341 ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta gttaatagtg
    14401 atacgatttt tttgatccag catcgcgccg agacggttga atgcagcagt gtcattgaca
    14461 tcgagattac aaaaatccag acgtgcactc agggtgtccc ataaaccttc atcaatggtt
    14521 tctttcatga aagtttcgag cgcctcgcgg acaactttgg tatatgccgc tttatcccag
    14581 tcagcacgcc ctacgccgat aatccgggtg tccgggttga gctgaccggc tttttccagt
    14641 tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc
    14701 aggtcacagg cctgggctgt ttgcgttacc gccatgtcat tctccttaag ttaactaacc
    14761 cggtacttaa gccagggtat acttgtaatt ttcttacggt gcactgtact gcttttccga
    14821 gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta cgggaaaagc gcgcaaagtg
    14881 cggcaaaact gataaaaaaa tcatcgtttt ccattcggta aaaatctgac actgctcatg
    14941 ttatgaaaaa aaaataacaa cttttttatc tgcttttgtc attaacacgg cacacaggcg
    15001 taatatctga caaaactgca tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga
    15061 gtctctttac atcatgaata tgctggaaaa aatccagtct cagctggaac atttgagcaa
    15121 atcagagcgc aaagttgccg aggtcattct ggcttcgccc gataacgcga tccattcgag
    15181 tattgctgct ctggcgctgg aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg
    15241 cagcatggac acgcgcggtt ttcctgattt taaacttcat ctggcacaaa gtctggcgaa
    15301 tggcactccc tatgttaatc gcaacgtcaa tgaagatgac agcgttgaat catacacggg
    15361 gaaaatattt gagtccgcaa tggcaacgct cgatcatgtc cgtcattcac tggataaatc
    15421 tgccatcaac cgcgccgtcg acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt
    15481 aggctcttct gccgccgttg cccacgatgc gatgaataag ttctttcgtt ttaatgttcc
    15541 ggtggtgtac tccgatgata tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg
    15601 agacgtggtg gtgctgattt ctcacacagg aagaacaaaa aatctggtcg agctggcgca
    15661 gctggcacgc gaaaacgacg ccatggtgat tgccctcacc tctgcgggta ccccgctcgc
    15721 ccgggaagca acgctggcaa ttaccctcga cgtaccggaa gatactgaca tttatatgcc
    15781 catggtttct cgacttgcac agctgaccgt gatagatgtg ctggcgacag gatttacttt
    15841 gcgacgcggt gcaaaattca gagataactt gaagcgggtc aaggaagcgc tgaaggaatc
    15901 gcgttttgat aagcagttac ttaatttaag tgacgatcgc taaaagcgac tgtcactgtc
    15961 ctaatcttat acgacatccg aatgagatta atttatcgcc atcgcggcgt tatttcattc
    16021 ggatttcatg ttcaagcaac acctggttgt ttcagtcaac ggagtattac atgtccaaaa
    16081 ggcttcgcag aacaaaaatc gttaccacgt taggcccagc aacagatcgc gataataatc
    16141 ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat gaacttttct cacggctcgc
    16201 ctgaagatca caaaatgcac gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc
    16261 atgtggctat tctgggtgac ctccaggggc ccaaaatccg tgtatccacc tttaaagaag
    16321 gcaaagtttt cctcaatatt ggggataaat tcctgctcga cgccaacctg ggtaaaggtg
    16381 aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg
    16441 gtgacatcct gctgctggac gatggtcgcg tccagttaaa agtactggaa gttcagggca
    16501 tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc caacaataaa ggtatcaaca
    16561 aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa agacaaagca gacattaaga
    16621 ctgcggcgtt gattggcgta gattacctgg ctgtctcctt cccacgctgt ggcgaagatc
    16681 tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg
    16741 ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga tgacatcatc ctcgcctctg
    16801 acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg
    16861 gcattcagaa agcgttgatc cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga
    16921 cccagatgat ggagtcaatg attactaacc cgatgccgac gcgtgcagaa gtcatggacg
    16981 tagcaaacgc cgttctggat ggtactgacg ctgtgatgct gtctgcagaa actgccgctg
    17041 ggcagtatcc gtcagaaact gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa
    17101 tcccgagcat caacgtttct aaacaccgtc tggacgttca gttcgacaat gtggaagaag
    17161 ctattgccat gtcggcaatg tacgcagcta accacctgaa gggcgttacg gcgatcatta
    17221 ccatgaccga atcgggtcgt accgcgctga tgacctcccg tatcagctct ggtctaccaa
    17281 ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac tgctctctat cgtggcgtta
    17341 cgccggtgca ctttgatagc gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc
    17401 tgctgcgcga taaaggttac ttgatgtctg gtgacctggt gattgtcacc cagggcgacg
    17461 tgatgagtac cgtgggttct actaatacca cgcgtatttt aacggtagag taagtacgtt
    17521 gccggatgcg gcgaaaacgc cacatccggc ctatagttca atgatagttc aacagatttc
    17581 gaatattctg aagcaaactt gaacttatca tcaggcgaag gcctctcctc gcgagaggct
    17641 tttttatttg atgggataaa gatctttgcg cttatacggc tggatttcgc ccggtttgcg
    17701 agttttcagc aattttagta tccaggtgta ttgttctggt cgcggaccaa caaaaatctc
    17761 gacttcttca ttcatccgcc gctcaatcgt atgatcatcc gcctctaaca gatcatccat
    17821 cggtgggcgc acctgaatcg tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg
    17881 tacaacgcgc gcacggcata ctttcatcaa acgaccaatc gcgggcaacg tcgctttata
    17941 ggtggcaaag aaatccacaa attcgctgtg ctctgggcca tgatcctgat cgggtaaata
    18001 atatccccag tacccctgac gtaccgactg gatgaatggt ttaataccgt catttctcgc
    18061 atgcagacga ccgccaaagc gacgacgcac cgtgttccag acataatcaa aaaccgggtt
    18121 gccctgatta tggaacatcg ctgccatttt ctgcccttgc gaggccatca gcatggcagg
    18181 aatatcgacg gcccaaccgt gcggcaccag aaagataact ttctcgttat tacgccgcat
    18241 ctcttcgatg atctccagcc cttgccagcc aacgcgcggc tgaattttct ccggcccgcg
    18301 tattgccaac tcagccatca ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac
    18361 aatcgcttcg cgttcagctt cactacgttc tggaaagcag agcgacagat tgattaacgc
    18421 acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg
    18481 atcacggaac tttggcggcg ttaaagcgat acccgccatc gctgctacac ccagccatgc
    18541 tccccagtag cgcgggtggc gaaaggattt atcaaactca ggaatgtatt cgctattatt
    18601 ttttttcgtt tccatgcttt tccagtttcg gataaggcaa aaatcaatct ggtgatagtg
    18661 tagcggcgca acttgccccg caccaaataa aaaaagccgg tactgactgc gtaccggctg
    18721 cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg
    18781 aattcgcgac gatcggagcc ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg
    18841 tttacggcct gctggtttat ccatacttca tagtgcagat gcggcccagt tgaacgtccg
    18901 gtattaccgg aaagcgcgat acggtcgcca cgtttcacct tctgtcccgg tttcaccaga
    18961 atcttgcgca agtgcatata acgcgtggtg tagctgcgac catgacgaat agccacataa
    19021 taacctgctg cgccactgcg tttggcaacc accacttcac cgtcacccac tgaaagcact
    19081 ggcgtacctt gtggcatggc gaaatcaaca cctctgtgtg gtgcaacgcg acctgtcacc
    19141 ggattagttc gacgcgggtt aaagttagac gagatacgga actgtttcgc cgtcgggaat
    19201 cgcaagaatc ctttcgccag accagtaccg ttacgatcgt agaatttgcc atcctcagcg
    19261 cggattgcgt aataatcttt accttctgaa cgcaaacgta cgcccagcag ctggctttgc
    19321 tcacgtttac catcaagcat ttctcgagac attaataccg caaattcatc gccttttttc
    19381 agtttgcgga aatccatttg ccactgcatg gctttaatca ctgcgctcac ttcggcgctg
    19441 gttaaaccgg cgtttctggc gctggcaaca aagctccctc cgacggtacc tttcagcaga
    19501 ttgttaaccc actctccttg ctgcatttcg ctggtcattt taaaaccgtt agcggcagta
    19561 cggtcatagg ttcgggtttc acgacgagac acttcccagg tgaggcgctg cagttcgccg
    19621 tccgcggtta atgtccagga gagttgttga ccgattttca ggttacgcaa ttctttgtcg
    19681 gcagcagcga gttgggtgat atcacccata tcaataccat actgattgag aatgctgctt
    19741 agcgtatcgc cagtggaaac aacatattca tgcacgcccg cttcaccggc gattttgtca
    19801 tccagttcgt cctggggaat ggcttcatct tcttgtgcag cttgatcaat cggctcactg
    19861 gcttcaggta agagcgaacg aatttcgttc tgttccagct caatggtttt gacaattggc
    19921 gtggcatcac ggtgataaac atagggccgc cagacagcga cggccagagt aagaacggtg
    19981 agcgacccca acataacgcg gtgtggtcgc ggtaaattat taaacgccag ggcgacagag
    20041 cgggctatct gttgcacgta atcacttcct cattaatctc ctttcaggca gctcgcatac
    20101 tggttggcta attgactcag gaattctgaa tagcttgttt tacccagttt gatattcgtc
    20161 cccaggggat ccaacgttcc catacgaacg gatgtccctc gtgcgacgct ctcaacgacc
    20221 gctggcctga actgtggctc agcaaaaacg caggttgctt tttgctcaac caactgtgtt
    20281 cttatttcat gtaaacgctg cgcgccaggt tgaatctcag ggttaacggt aaaatgacca
    20341 agcggtgtca gtccgaactg tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa
    20401 cctttcccct tgagcggcgc gagctcgtta ccaacctgcg tttcggttga ggccaattgt
    20461 gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac tttgcggcat aagttccact
    20521 aattttccat ggattgcaac cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc
    20581 atgttgaaat cgccgtgatg gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca
    20641 tcatcgccgt gaatactttt catcagtagc ggtttcacat cttcaagctg cgcaatcgtt
    20701 acctgcttcg cttctggtaa tttgcttacc ggtttttgca taaacgcttc catctccggg
    20761 ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta catccgatgg acgcagtgaa
    20821 taatcatgtt ctgaagcgcc gtcaggcagt aaaacctccg tttctgttac accatcagca
    20881 atggcagaag cgatgaaccc aacgggttta agcgaagcga caacggcggc atctgcggcc
    20941 tgtgttgcac ctccccagag agcggcggat aatgctgcga aaagaagcgt ttttttatgt
    21001 aacataatgc gaccaatcat cgtaatgaat atgagaagtg tgatattata acatttcatg
    21061 actactgcaa gactaaaatt aacatgacaa gtctggtttc cctggaaaat gtctcggttt
    21121 cttttggcca acgccgcgtc ctctctgatg tgtcgctgga acttaaacct ggaaaaattt
    21181 tgactttact tgggccaaat ggcgcaggta agtcgacact ggtacgggta gtgctcgggc
    21241 tggtaacacc cgatgaaggg gttatcaaac gcaacggaaa actgcgcatc ggctatgtac
    21301 cgcagaagct gtatctcgac accacgttgc cactgaccgt aaaccgtttt ttacgcttac
    21361 gccctggtac acataaagaa gatattttgc ctgcactgaa acgtgtccag gccgggcacc
    21421 tgattaacgc accgatgcaa aagctctctg gtggcgaaac gcagcgtgta ctattagcgc
    21481 gagcattgtt aaattgcccg caattattag tgctggatga acccactcaa ggcgtggatg
    21541 taaatggcca agtggcgtta tatgacctta ttgaccagtt gcgtcgtgaa ctggattgtg
    21601 gcgttttaat ggtttctcac gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt
    21661 gcctgaatca ccacatttgt tgttccggca caccggaagt tgtttccctg catccggagt
    21721 ttatttcaat gtttggtcct cgtggtgctg aacaactggg tatctatcgc catcatcata
    21781 atcatcgtca cgatttacag ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat
    21841 tgaattatta tttcccggtt ggttagccgg gatcatgctc gcctgtgccg cgggtccgct
    21901 gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc
    21961 attacttggt gtcgcgtttg gtttgttgct ggacgtgaat ccattctatg cggtgattgc
    22021 cgttacgctg ctgctggcgg gcggtctggt atggctggag aagcgtccac agctggcgat
    22081 cgacacgtta ttagggatta tggcgcacag tgccctgtcg ctgggcctag tggtcgttag
    22141 tctgatgtct aacattcgtg ttgatttgat ggcttacctg ttcggtgatt tactggcagt
    22201 gacgccagaa gatctcatct ctattgcgat tggcgtggtc atcgtggtgg ctattttgtt
    22261 ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg gatctggcgt ttgttgatgg
    22321 tgtgaaatta cagcaagtga aattgttgtt gatgctggtg acggcattga cgattggtgt
    22381 agcgatgaaa tttgtcggtg cgttgattat tacttcgttg ctgattattc ctgcagctac
    22441 tgcgcgtcgc tttgcccgta cgccggaaca gatggctggt gtcgctgttt tggtggggat
    22501 ggtggcagta actggcggtt taaccttttc cgcgttttac gatacgccgg cgggtccgtc
    22561 ggtggtgcta tgtgcggcac tgttatttat tatcagtatg atgaaaaagc aggccagcta
    22621 atctatcgct gaacacattt gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg
    22681 ctatctctgg ctggcctgat aagacgcgaa cagtgtcgca tcagacacac tgccagtgtc
    22741 ggatgcggcg cgagcgacca atccgactta cggcatttct ggcggcgtta tgccaaagtg
    22801 attccacgcc cgcgtcgtcg ccatacgccc gcgcggtgta cgctgcaaaa agccttgctg
    22861 aatcaaataa ggttccagta catcttcaat ggtttcacgt tcttcgccaa tggctgccgc
    22921 caggttatcc agacctaccg gcccaccaaa gaacttatcg attaccgcca gcaacaattt
    22981 gcggtccata taatcgaaac cttcagcatc gacattcaac atatccagcg cctgagcagc
    23041 gatatctgcc gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag
    23101 cagacggttg gcaatacgtg gcgtaccgcg agcgcgacga gctacttcct gcgcaccgtc
    23161 atcactcatc tcaagcccca taaagcgtgc actgcgactg acgatatatt gcagatccgg
    23221 cacctgataa aactccagac gttgcacaat accaaaacgg tcgcgcaacg gtgatgtcag
    23281 cgaacctgcg cgcgtggttg cgccaatcag ggtaaacggc ggcaaatcaa ttttaatgga
    23341 gcgtgccgcc ggaccttcac caatcatgat atccagttgg tagtcttcca ttgccgggta
    23401 cagcacttct tcaacaactg gcgataaacg gtggatctca tcaataaaca gcacgtcatg
    23461 cggttcaagg ttagtgagca tcgcagccaa atcgcccgcc ttttccagca ccggaccaga
    23521 agtcgtgcgt aaattaacgc ccatttcatt ggcgacaatg ttggcaagcg tagttttacc
    23581 caaccccgga ggaccaaaaa tcaacaaatg atcgagggca tcgccgcgca gtttcgctgc
    23641 tttgatgaaa atctccatct gtgaacgaac ctgcggctga ccaacatact cttccagtaa
    23701 tttagggcga atggcgcgat ctgctacatc ttccggcaaa gtggtaccgg cagaaatcag
    23761 acggtctgct tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat
    23821 gtttcactgc tggcgtcagg gcgagcgatt ttgctcacca tgcggcttgc ttcttgtggt
    23881 ttatagccta gcgccaccag cgcggcaact gcttcttgtt cagcatcgtc ggtcgccggg
    23941 ctggcaggag acgtgagtac caggtcggcg gctggcgtaa agagatcgcc atgcaaacct
    24001 ttaaatcggt ctttcatttc aacaatcaag cgttcggcgg tttttttgcc aatacccggc
    24061 agtttcacca gtgcccccac ttcttcacgc tcaacggcat taacgaactg ctgcgctgac
    24121 attccggaga ggatcgccag ggccaacttc gggccgacgc cgttggtttt gatcaactct
    24181 ttgaacaacg tgcgttcttg tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc
    24241 accacaaagt gggtgaaaac gatcgcttcc tgacccgctt cagggagttc ataaaaacag
    24301 gtcatcggca tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt
    24361 tgtttttcaa tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat
    24421 caaagataat gctgtatgat aaaaaaatgc tggatagata tccagcgtag gatgaagaaa
    24481 acttgcgagg tgtcccgatg atctgaaaaa tggcacagta taatttattc aacaggttat
    24541 attggaagca aatatttgaa tattacatat tcagtgaaga aatgcgtaat aaaaatatac
    24601 attgcgactc ctgaaaaaat aattttttta tgctattacg tatattcgta tctatttcaa
    24661 tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga tatcgtcttt
    24721 acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggagtaat
    24781 cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt
    24841 cccctttcaa ccatcaccac g
//
LOCUS       DADLZM010000050        24819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLZM010000050 DADLZM010000000
VERSION     DADLZM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.050"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..372
                     /locus_tag="JNY73_001974"
     CDS             <1..372
                     /locus_tag="JNY73_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048000.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            483..1406
                     /locus_tag="JNY73_001975"
     CDS             483..1406
                     /locus_tag="JNY73_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8048001.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVKGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1410..2228)
                     /gene="nlpA"
                     /locus_tag="JNY73_001976"
     CDS             complement(1410..2228)
                     /gene="nlpA"
                     /locus_tag="JNY73_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY8048002.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2450..2743
                     /locus_tag="JNY73_001977"
     CDS             2450..2743
                     /locus_tag="JNY73_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048003.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2784..3974)
                     /gene="nepI"
                     /locus_tag="JNY73_001978"
     CDS             complement(2784..3974)
                     /gene="nepI"
                     /locus_tag="JNY73_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY8048004.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLQV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4185..4637)
                     /locus_tag="JNY73_001979"
     CDS             complement(4185..4637)
                     /locus_tag="JNY73_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY8048005.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHPPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4690..6024)
                     /locus_tag="JNY73_001980"
     CDS             complement(4690..6024)
                     /locus_tag="JNY73_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8048006.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6199..7965
                     /gene="adeD"
                     /locus_tag="JNY73_001981"
     CDS             6199..7965
                     /gene="adeD"
                     /locus_tag="JNY73_001981"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY8048007.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8011..9402)
                     /gene="uhpT"
                     /locus_tag="JNY73_001982"
     CDS             complement(8011..9402)
                     /gene="uhpT"
                     /locus_tag="JNY73_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY8048008.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9540..10859)
                     /gene="uhpC"
                     /locus_tag="JNY73_001983"
     CDS             complement(9540..10859)
                     /gene="uhpC"
                     /locus_tag="JNY73_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY8048009.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(10869..12371)
                     /gene="uhpB"
                     /locus_tag="JNY73_001984"
     CDS             complement(10869..12371)
                     /gene="uhpB"
                     /locus_tag="JNY73_001984"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY8048010.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12371..12961)
                     /gene="uhpA"
                     /locus_tag="JNY73_001985"
     CDS             complement(12371..12961)
                     /gene="uhpA"
                     /locus_tag="JNY73_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY8048011.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13036..13326)
                     /locus_tag="JNY73_001986"
     CDS             complement(13036..13326)
                     /locus_tag="JNY73_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY8048012.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13330..15018)
                     /gene="ilvB"
                     /locus_tag="JNY73_001987"
     CDS             complement(13330..15018)
                     /gene="ilvB"
                     /locus_tag="JNY73_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY8048013.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPVAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPLGAANTEMVGE"
     gene            complement(15124..15222)
                     /gene="ivbL"
                     /locus_tag="JNY73_001988"
     CDS             complement(15124..15222)
                     /gene="ivbL"
                     /locus_tag="JNY73_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY8048014.1"
                     /translation="MTTSMFNVKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15787..15876
                     /gene="tisB"
                     /locus_tag="JNY73_001989"
     CDS             15787..15876
                     /gene="tisB"
                     /locus_tag="JNY73_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY8048015.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16156..17340
                     /gene="emrD"
                     /locus_tag="JNY73_001990"
     CDS             16156..17340
                     /gene="emrD"
                     /locus_tag="JNY73_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY8048016.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17348..17845)
                     /locus_tag="JNY73_001991"
     CDS             complement(17348..17845)
                     /locus_tag="JNY73_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY8048017.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPSRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17842..18204)
                     /locus_tag="JNY73_001992"
     CDS             complement(17842..18204)
                     /locus_tag="JNY73_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY8048018.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18194..18541)
                     /locus_tag="JNY73_001993"
     CDS             complement(18194..18541)
                     /locus_tag="JNY73_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY8048019.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18650..19063
                     /locus_tag="JNY73_001994"
     CDS             18650..19063
                     /locus_tag="JNY73_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048020.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19110..20576)
                     /locus_tag="JNY73_001995"
     CDS             complement(19110..20576)
                     /locus_tag="JNY73_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY8048021.1"
                     /translation="MTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCT
                     PARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGT
                     GECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNR
                     AVDFLQQPVRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPE
                     HHRLWAQAMLSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDH
                     GEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEK
                     PEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDEL
                     YDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPR
                     WMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20587..22302)
                     /locus_tag="JNY73_001996"
                     /pseudo
     CDS             complement(20587..22302)
                     /locus_tag="JNY73_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22469..23362
                     /locus_tag="JNY73_001997"
     CDS             22469..23362
                     /locus_tag="JNY73_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8048022.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23359..24173)
                     /locus_tag="JNY73_001998"
                     /pseudo
     CDS             complement(23359..24173)
                     /locus_tag="JNY73_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(24173..>24819)
                     /locus_tag="JNY73_001999"
     CDS             complement(24173..>24819)
                     /locus_tag="JNY73_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY8048023.1"
                     /translation="EPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLID
                     QVLPQNWIPMFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLY
                     SKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRLRIALHDMSQTL
                     DDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV"
BASE COUNT         6246 a         6646 c         6184 g         5743 t
ORIGIN      
        1 atcgcaggct attacatgaa acgtattggc aagcgactat taatgctcat tgctatcgtg
       61 agtggtatgt gtttttacgc cagcgtactc atggcgacga ctccggcggt tgagctggaa
      121 ttgcaaattc taaatgccat cttccttggt attctctgtg gtatcggcat gctttatttt
      181 caggacttga tgcctgaaaa aataggctct gcgacaacgt tatatgcaaa tacttcacgc
      241 gtcggctgga ttatcgccgg ctctgttgac ggaattatgg ttgaaatctg gagctaccat
      301 gcgttgttct ggctggcgat agggatgttg ggtattgcga tgatttgcct gctgtttatt
      361 aaagatattt agctcaccag tctgggctaa gaaaaatcgg cgtggcgcaa acagagccat
      421 cctcgccatt tttgctatca tgcctgcata cataaacgac aaaacagtat gcagagggaa
      481 aaatgggttc caccagaaag gggatgctga acgttctgat tgccgccgtg ttgtggggaa
      541 gttcaggggt ctgtgcgcaa tacatcatgg agcaaagcca gatgtcgtcg cagtttttga
      601 ctatgacgcg tttgatattc gccggtttga ttctgctgac gctgtcattt gttcatggcg
      661 ataaaatctt ttctattatt aacaatcaca aagatgccat tagtctgctg attttttccg
      721 tggttggcgc gctaaccgta cagctcactt ttttgctaac catcgaaaaa tcgaacgcag
      781 ccacggcaac ggtgctgcaa ttcctctcac cgacgattat cgtcgcctgg ttctcactgg
      841 tacgtaaatc gcgcccgggc attctggttt tctgcgctat tttgacatcg ctggtcggga
      901 cttttttatt ggtgacacac ggtaatccga cgtcattatc gatctctcct gccgcgttat
      961 tctggggcat tgcctcggca tttgctgctg cattctatac cacctatccc tcaacgctga
     1021 ttgcccgcta tggcacgttg cctgtcgtcg gctggagtat gctgatcggc ggtctgattc
     1081 tattgccttt ttatgccaga caagggacaa actttgtcgt taaaggcagt ttgattttgg
     1141 cgttttttta tttgatcgtc attggtacgt ccctgacatt tagtctgtac ctgaaaggag
     1201 cacaattaat tggcggtcca aaagccagca ttttgagctg tgcagaacca ttaagtagcg
     1261 cgctactctc tttgctgttg ctggggatca cgttcacatt accggactgg ctgggaacgc
     1321 tgctgattct gtcatcggta attttgattt caatggattc ccgtcgccgc gccagaaaaa
     1381 taaatcgtcc ggcgcgggat gagtgagaat taccagccag gcaccgcgcc accgttaaag
     1441 atggtttctg ccgctttagc gacttcggat gattgataag attgcagaaa ttccttcacg
     1501 ttttctgcat tcttattatc ttcccgtgcc accaaaatat tcacatacgg cgaattttta
     1561 tcttcaataa atacactgtc gtgcaccgga gaaagcccgg tctgctgaat gtaagtggtg
     1621 ctgataatcg ccacatcaac tttgggatca tccagtacgc gcgggagctg cgccccctcg
     1681 agttccataa tctgcaaatg acgaggatta tcggttatat ccagcgcggt aggtaataac
     1741 ccttttccct ctttcagagt aattagcttc tctttttgca acagtaaaag cgcacgacca
     1801 aggttggtcg ggtcgttggg gatcgccact gtcgcgcctt cttttatttg cgccacggtt
     1861 ttgatttttt tggaataacc cgccatgggg aagacaaacg tatttcccac cgctaccagt
     1921 ttataaccat gcgcctgatt atcttgttca agaaagggtc gatgctggaa gacgttcgca
     1981 tcaagttcgc catgatttgt cgcatcgttc ggtagcaacg aaccgctaaa tcctaccagt
     2041 tgaacatcga ggccatattt ctcttttgcc acctttttag cgacttccgc gacatcttgt
     2101 tctgcgccat ttataacgcc aactttaatg tgttttgcat cgctgctact ctggtcgcaa
     2161 cctgccagca gaattccggc cagcaataat gcggcccctg tccgtagatg atgtgttgtc
     2221 agtttcattt ttttatcctt ttattaatga ttacctgact atagcggtaa ggtcgctgcg
     2281 gtttaaaaaa cgaaacgcta tcgataagaa taaaaaggaa taaaagtgga atataaagtc
     2341 tggcacttct tactgaccac gcaagcgcgt ttcgtacagc atgatgagag cgatgaaagc
     2401 aaactacact tatgcttcat tcgatacaca tttgtaaaag gttgaatcca tgaaaccaac
     2461 gacactactg cttattttca cttttttcgc tatgccaggc atcgtatacg ccgagtcgcc
     2521 ttttagctca ctgcagtccg caaaagagaa aaccacggta ttacaagatt tacgtaaaat
     2581 ttgcacgcca caggcgtcat tatcagatga agcgtgggaa aagttaatgt tgtcagatga
     2641 aaacaataaa caacatatac gcgaggccat cgtggcgatg gaacgtaata atcagagcaa
     2701 ttattgggaa gcgttgggta aggtagaatg cccggatatg taagaacagc aacaactact
     2761 gaccaacgct tctttacctg atatcaggat ttcttcattt tcacctttgc agtaaccaac
     2821 aatgcagtca gcaacatcaa tgtgccggac aacatcaacg gcgaagtcag accaatatta
     2881 tccagcgcat aaccgccgat tgccgcgcca caggtattag caagctgaat aaccgccacc
     2941 tgaatagacc cggctttttc tgcctgatcg gccagcgagc gggtgatcca cgttgaccag
     3001 ccgacgggaa ccaatgcaaa agttagcccc cagataatcg ccacgccggt agcaacgatt
     3061 ttatcgcttc cccacaacgt cagtaccaac gcactcacag ccagtattaa cggcgcgcct
     3121 gctaaggcca gttttaccga acgtttaaga atgaacgacg aaagcgacgt accaataaag
     3181 ctggcgatac caaaactcaa cagcaccagc gttaagccat ccacgccgaa tcccgccagg
     3241 ttcatataca ccgggcgaat atacgtgaag aaagcaaact gcccggcgaa agacatgaag
     3301 atggcgatca tccctgccat cacacccgga cgttgtaata agcggaaagt attttgtttc
     3361 tgatgcgagg gttcgcctgg cagtgaaggc aatgatttga taatccagaa aatacacagc
     3421 acgcccatca ccgccgccgc attaaagaca ttgcgccaac cgataagctc gcctaaaaaa
     3481 ctgcccaacg gcgcggcaat caccagcgca atagaaaccg cgccgaagat caccgacagc
     3541 gccttcggca ccgtacgcgg cggtaccaga cgcatggtca gcgacgccga catcgcccag
     3601 aacccgccca gcgccagccc cagacaggca cgaccgatta aaagcaaact gaatgagtta
     3661 gcaaaggaaa cctgcaagca ggagagcgtc agcaaaacgg caaacaaaat aacaacgtag
     3721 cggcggtcag tggcctgaat tgtctgggta ataaacaaac tggcaaacat tgccacaaag
     3781 gcggtcacgg tcaccgattg cccggcaacc ccttccgaaa tgcctaaatc ctgggccatt
     3841 ggcgtcaaca aactgacggg caaaaactca acgataatca gacaggcgac acaaaacgcc
     3901 accgagaaaa cggctgacca gttcggtcgg gtgatggcat ccgcgccgcg gttttcggca
     3961 ataaattcac tcatggtgtt acccgtggca tgtttcaggg gaaaagccga ccactttaac
     4021 attgaaatgt gtgacgcatt taacgttttt gcaactttta cggtgttgct tgagctggag
     4081 ttaagatcga aacggaacag ggcaaagatt accggatgcg gcgtgaacgt cttatccacc
     4141 cgacaggtca ggcgcggtct gtaggctgat aagacgaaac ggtttcaatg caaccagtga
     4201 acgccctcgg caggctgaaa gaacgcgtta tagcgcatct gaaaagcatt aatatcctgc
     4261 atttcaggtt cggtttcacg cagaaaaccc agcgccagcc tgacctgagc atcggtaatc
     4321 ggcgcggaaa ggcgggcttt ttgtaaaatc cggtgccatt gcagcaggtt ctgttcgcta
     4381 tcatcgacaa tgacaatttg ccagataagc aacacctgcg cggcgttttg caggtgcgac
     4441 tcatccgggg gatgcaaata acgtaaaaat tcgtcaccgg ctgactttcc catttgatcg
     4501 accatcgact ccacgggtac gacatcgata ttcagccgat cgctgagacg gcgaatcgct
     4561 tttctggccc cggatgtcag cacccgaaaa ccaaccacaa acactaccgc cagcgtggcg
     4621 agcattatcc agatcatgag ttctcctgcg taatcagaac tcatgatatc tggaaaccct
     4681 cgccagttac taatccacca gaataatctt caatgcgaac agagctgcga ccaccaccac
     4741 acacaggttc agatcgcgcc agcgcccggt gcacactttc atgatgcagt acgacataaa
     4801 gccgagtgca atcccttcgg tgatcgagaa agtaaagggc atcatcaccg tggtaataaa
     4861 cgcaggcacc gattcggtaa aatcatccca gttaacgcgc gccaggctcg aagtcatcag
     4921 cacgccaaca aagattaacg ctccagcggt tgcgtaagga ggaaccatcg ccaccagcgg
     4981 tgagaagaac ataactaaca ggaacataac gccgaccacg acagccgtta gccccgtgcg
     5041 accaccgact gccacaccag aagtactttc aatataggca gtaacagacg aggtaccgat
     5101 aaatgcgcct gccaccgaac tgacgctatc aacatacagc gccttattca tattggggaa
     5161 tttaccgttg ctatctatta agcccgcttt atcagttaca ccaattaatg ttcctgatga
     5221 atcaaatagg ttgatcagca taaaggagaa aatgataccg gcgagttcaa gtgttaacgc
     5281 gccgctcaaa tctacttcac caatgacgcc gctaatatca ggcggaatgg aatagacgcc
     5341 gctaaaatga acatcaccga aaaataatcc acagcaagac gtcaccacaa tagaaaccag
     5401 caccgcggca tgaaaatgac gtgatgacaa cacggttata ataaaaaacc ctaaaatacc
     5461 taacaacacg ccgtgagaac ttaaatcgcc aatcatcacc agcgtgtctt tattggcgac
     5521 aataacacca gtgtttttta atcccattaa ggcaataaat aatccgattc cactggtgat
     5581 accaatacgt aaacttaatg gaatgttgga gatcatccag taccggatac gaaacagcgt
     5641 gagcaaaaat agcccaattg cgccccagaa tatcgcgccc atcccggtct gccaggagat
     5701 ccccatcgcc cccacgacca cgaacgcaaa gaaggcgttc agccccattg ccggagccag
     5761 caccacgggt aagttagcaa atatccccat cgcaatactg ccgataccgg caatcaaaca
     5821 ggtggtaaca aacaccactt tcgggtccat ttgtgccgcg ccgaggattt gcgggttcac
     5881 aaaaacaatg tacaccatgg ttaaaaaagt ggtcatcccc gcaatcaatt ctgtgcggac
     5941 ggtggtccca tgctgaggta gtttaaataa tcgcgaaagc gtccctgatt cattactcac
     6001 gtaatcggta ttgtcattat tcattttttt gtccattgga ggagatttaa tcccttgctt
     6061 ttgtaaaaca aatgacaaca acggttcagt gataattatc acatttcaat tgcacattaa
     6121 tagatattct ttaataatct cgcgacgttt ctttatgata aataataatc aaattgataa
     6181 aatcaaaatg agaaaaatat gaataattcc attaaccata aatttcatca cattagccgg
     6241 gctgaatacc aggaattgtt agccgtttcc cgtggcgacg ctgttgccga ttatattatt
     6301 gataatgtct ctattctcga cctgatcaat gccggagaaa tttccggccc aattgtgatt
     6361 aaaggacgtt acattgccgg tgttggcgca gaatacgctg atactccggc tttgcagcgg
     6421 attgatgctc gcggcgcaac ggcggtgcca gggtttattg atgctcacct gcatattgaa
     6481 tccagcatga tgacgccggt cacttttgaa accgctaccc tgccgcgcgg cctgacgacc
     6541 gttatttgcg accctcatga aatcgtcaac gtgatgggag aagccggatt cgcctggttt
     6601 gcccgctgtg ccgaacaggc aaggcaaaac cagtacttac aggtcagctc ttgcgtgccc
     6661 gctctggaag gctgcgatgt taacggtgcc agttttaccc ttgaacagat gctcgcctgg
     6721 cgggaccatc cgcaggttac cggccttgca gaaatgatgg actaccctgg cgtaattaac
     6781 gggcagaatg cgctgctcga taaactcgat gcatttcgct acctgacgct ggacggtcac
     6841 tgtccgggtt tgggtggcaa agaacttaac gcctatattg ctgcgggtat tgaaaactgc
     6901 cacgaaagtt atcagctgga agaaggacgc cggaaattac aactcggcat gtcgttgatg
     6961 atccgcgaag ggtccgctgc ccgcaatctc aacgcactgg caccgttgat caacgaattt
     7021 aacagcccgc aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa
     7081 gggcatatcg atgccttaat tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg
     7141 gcatatcgcg tcgccagctg gtcgacggcg cgccactttg gtctgaatca cctcggctta
     7201 ctggcacccg gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg
     7261 cagcaggtac tggtgaaagg cgaaccgatt gatgcgcaaa ccttacaggc ggaagagtcg
     7321 gcgagactgg cgctgtctgc cccgccatat ggcaatacca ttgcccgcca gccagtttcc
     7381 gccagcgact ttgccctgca attcacgccc ggaaaacgct atcgggtcat tgacgtcatc
     7441 cataacgaat tgattacgca ctcccgctcc agcgtctaca gcgaaaatgg ttttgatcgc
     7501 gatgatgtgt gctttattgc cgtacttgag cgttacgggc aacggctggc tccggcctgt
     7561 ggtttgctcg gcggctttgg cctgaatgaa ggcgcgctgg ctgcgacagt cagccatgac
     7621 agccataata ttgtggtgat cggtcgcagt gccgaagaga tggcgctggc ggtcaatcag
     7681 gtgattcagg atggcggcgg gctgtgcgtg gtacgtaacg gccaagtaca aagtcatctg
     7741 ccgttaccca ttgccgggct gatgagcacc gacacggcgc agtcgctggc ggaacaaatt
     7801 gacgccttga aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc gtttattcag
     7861 atggcgtttc tttctctgcc agtgatcccc gcgctaaaac taaccagtca ggggctattt
     7921 gatggcgaga agtttgcctt cactacgctg gaagtcacgg aataataaaa aaagcccggc
     7981 gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt
     8041 ctcgcggcgg attttgcgtt cttccattac cgctactatc gccatcaggc agatacaacc
     8101 aatcgcggcg atatccagcg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg
     8161 cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc
     8221 aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc
     8281 agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc
     8341 cagatagata tattcgttac tggcatgttg atacacaccg agcgtggcga taatcagcgc
     8401 cagcgcgatg caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca
     8461 cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc
     8521 tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg
     8581 taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac
     8641 atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc
     8701 gctgacctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc
     8761 gctaccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa
     8821 catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca cacctgctgc
     8881 gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg
     8941 acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa
     9001 gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact
     9061 ggcactgaag cccagcatac aaatggcaga gaggataagc atgaacggca ggaattgttt
     9121 ggtgtttttg ccgtcggcgt agtatgaaac caacgtttta cccacgccat aagtgatgga
     9181 gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg aaatcatatc
     9241 gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac
     9301 caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag
     9361 gtccagggtc ggcttgcgaa cctggtttag gaaagccagc atgggttact cctgaaatga
     9421 atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     9481 aagaaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat
     9541 cacgcttcac gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg
     9601 gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag cactttcgcc
     9661 agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     9721 gcccctgccg cctctttgtg ggaacactct gccgccgcca taccaattaa catctgcggg
     9781 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     9841 ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     9901 ggcccacggt tgccgttaaa caatttgtcc gagccccaac cggctaccag cgcaccgata
     9961 aatccgccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc
    10021 gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc
    10081 acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc
    10141 tctttacgcg tcaaccctgc cccttcttgt tgttgagcaa tttccagcgc gtcgtgccgc
    10201 cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgtagccg ccagcagaga
    10261 aaaatcccca cgactatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa
    10321 tgcagcgcgc tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg
    10381 ttccataatg cccaccaacc gccgcgctcg gtacgtgaat accaggccgt taacagacgc
    10441 gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag cacggcaaac
    10501 gcccatagcg acgtcgagaa gccaaacaga atgttcatga tgcccgtggc gataagccct
    10561 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
    10621 acgccgtaag taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg
    10681 ccgttagcaa ggatttctgg taccgcggcg ttaaaacttt tccgcgtgaa gtaaaacagc
    10741 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata acgggcatca
    10801 atttcatgtt tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
    10861 atcgaacctt agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
    10921 gaaatgtgta atgtgccgcc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa
    10981 ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac cagcatcaac
    11041 cgttcatcct gctgccagcc ttgcagcgta accgcgctgg catcggcatg tttcacaatg
    11101 ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaatgct
    11161 gattcatcga tacgccattc gagatggctg acaatgccgc gcccttccag ctccatttcc
    11221 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac
    11281 cgtcctaaca aacggcgcac cgcgtcgtaa acgcccagcg acagttgttc gatgagctgc
    11341 ccgctctgct tcacactggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta
    11401 cgaatagcag tgatggtctg accgatatcg tcgtgcagct cacgcgccac gtcgcggcgc
    11461 acgctctctt cggtttctaa caaacgttca gccagatgct gattgcgcgc cagttccttt
    11521 tgtagcgact ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc
    11581 agactttgca ccagcaacga gagtaataaa tccaccagat gatcgcgcca ggtttgactg
    11641 gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ctccttgcca gccatagtgc
    11701 caggccagcg cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtcc
    11761 ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca gaccagatgc
    11821 cgcccgcgcc agttgatggg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta
    11881 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcagacc gccagttaaa
    11941 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
    12001 aacgccgccg ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc acgctgatgg
    12061 cgatagcgcg agatcagcgc tacgggcagt aacgtcagta aactaccgat catcaataac
    12121 ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcaccc
    12181 agcaatacgg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
    12241 ggaaataaca gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccatagg
    12301 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
    12361 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc
    12421 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt
    12481 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac
    12541 gtttggtcag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
    12601 aacagccgcc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt
    12661 tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt
    12721 gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
    12781 agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg
    12841 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
    12901 gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca
    12961 tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt
    13021 tcaagccttg agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga
    13081 ctgattacgc tgcactttca cgacatcttc cagcttatcg atttggctta tcatctgctc
    13141 cagacgctgg tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg
    13201 cagacaaaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt
    13261 cattacgccc ggatggttgc gaacggtgag ctccagaatt acgttgttat gagttgtgtt
    13321 ttgcatggct tattccccca ccatttcagt attcgccgca cctagcggca ccatcgggta
    13381 aactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgattat
    13441 ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa
    13501 tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg
    13561 ctcgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat
    13621 gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag
    13681 gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat
    13741 cgccacaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg
    13801 gcgattgagc ggataagctt gcgcggtcca catctgatgc tgacccacat cggtggtgat
    13861 aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg
    13921 atcgcacgct ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta ccaactggtg
    13981 ccactctgca cgcagttgcg cttccacctg cgggatcaat tgcgccagca cgtcatcaac
    14041 atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcgc gatcgatatc
    14101 gacatgaata attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc
    14161 aaaacgcgca ccgagcacaa tcaacaaatc cgcctcctgc aaaatatagt tggtactgcg
    14221 cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag
    14281 cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc
    14341 aggcgcattg atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat
    14401 tgtagctgcg tcacgaatgc tttcttcgct aaaggagggt gcagcggctt tttctgccac
    14461 cgcgggctga gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa tgtctatcca
    14521 caccgggcta gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgtgggag
    14581 ctcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt
    14641 gtccacttcc tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca
    14701 aatcagcggg atggagtcca gccgcgcatc agcaatggcg gtcaccaggt tagtcgcacc
    14761 cggcccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc
    14821 gataaacccc gcgccctgtt catggcgagc cagaatatgg cggatttgcg tgctttggct
    14881 taaggcgtcg taaacaggca ggatagaacc gcccggaatg cccgtcacaa tcttaatgcc
    14941 ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt
    15001 tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct
    15061 taaacgaaaa accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag
    15121 tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg
    15181 cgcagttggt agtagtttta cgttgaacat ggaagtagtc attggggacc ttgttggttt
    15241 tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga aaatttttca
    15301 gcaagggaaa attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc
    15361 cgccggagcg aggtttcgtc agccgcctgc ggctggtaac cgcaaagcac actgtattat
    15421 gtcaacactg aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac
    15481 gtatttagtg catagttgag tatcgttcac agtttgcgtt ttgtccaaat attactgttt
    15541 atttatacag taaacttcta taatatcact gttcgcaatg tgttatgcgg gggccgcatc
    15601 gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc
    15661 gtgagcacac tgtgttatgt caacaagcac aacgtttctc cttgagatac cgcgtgcaca
    15721 acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag
    15781 acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg
    15841 caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg
    15901 ggagcgggta accgctcccc ttttatattt agcgtgcggg ttggagtcgg atgcgatgct
    15961 gacgcatctt atccgcccta ccatccctcc cggcaacatt tattgccgct tttgtttaca
    16021 tattctgccg ctaaacaatt ccccattcct ggcgtatatc tggctaacat tcatcaatgt
    16081 gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttattctaat tatttttata
    16141 aaagcatccg tgataatgaa aaggcaaaga aacgtcaatt tgttattgat gttggtatta
    16201 ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg
    16261 cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact
    16321 tacggtgtct cacagctgtt ttatggcccg atatccgacc gtgtcggtcg ccgaccagtg
    16381 atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt
    16441 ttgacggtat tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta
    16501 atggcgcgta ctttgccgcg tgatttatat aaacggacac agttgcgcca tgctaacagc
    16561 ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg
    16621 gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg
    16681 accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc
    16741 ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg
    16801 ctgattggcg gtctggccgg gattgctgcc tttgaagcct gctccggcgt gctgatgggc
    16861 gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggcg
    16921 gcattttttg gggcatggtt tgccgggcgt cctaataaac gcttctcaac gttgatgtgg
    16981 cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc
    17041 gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg
    17101 ctgtttccgc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct
    17161 ggtgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg
    17221 atgttgccgc aaaccggtca gggcagcctg gggttgttga tgaccttaat gggattgttg
    17281 atcgtgctgt gctggctgcc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa
    17341 gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat
    17401 cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa
    17461 gcacgctgtc tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct
    17521 ggcagtcacg gctcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa
    17581 aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcaaac agggcaatta
    17641 aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa
    17701 attcaagatg gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa
    17761 aatcggcagg ttgcagagca tccttgtact ctacaaccag cttatgcctg tcctcttcag
    17821 catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat
    17881 atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc
    17941 acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg
    18001 ggtgtagtgc cagaggatca gcgccacgat ggcgaggatg ccaatcgaaa tccagaataa
    18061 catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag
    18121 catggtacga aaccacgcca gtgatgtgcg ctccggctgc agccctgggt cggcaatgcg
    18181 tctggcttta cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacaacca
    18241 tcaatattaa gctgatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca
    18301 tcgctttttc attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca
    18361 gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt
    18421 gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac gccagaaaag
    18481 tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca
    18541 ttgttgctcc tttgcaattt cactaacgct ctatctttag cggttttttt taagaatttc
    18601 gtccgcccgt ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat
    18661 agcgcaaaat aaaatcagtt cattgggcat gctgttcggt gccatcgctt taatgatggg
    18721 gattatccac ttttcattcg gccctttttc cgcaccaccg ccaacgcttg aaagcatcgt
    18781 ggcggacaaa acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag gcgaaaaaat
    18841 taccactgtg gagaaaaaag aggacatgga tatcgataaa atcctcgatc aaagcggtat
    18901 cgcactggcc attgctgcgc tactttgcgc atttattggc ggtatgcgta aggaaaatcg
    18961 ctggggcatt cgcggcgcgc tggtttttgg tatcggcatc gtgtgcagca ttttgctgat
    19021 attcctgatt ttttccttcc tgaccggcgg ttcgctggtt taagcagcct gaataatgcc
    19081 cgccgggtga tcatcagcgg gcattatcgt cagaatttct gttttttctc ctccaccttc
    19141 actccttgcg tcggtaggcc ggtgtcatag tcgcgtacca ctggcgaata gccatcttgt
    19201 gggcgtgggc gaaacgcccc catccagcgc ggtcgtgcat ctttacgcca cggacgcaga
    19261 ctccattggt aactgcggaa cggatcgcga attttgtcca tgtaatccaa taaggcgtca
    19321 tgcattttgc tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg catttcattt
    19381 gggtcattac ggcgatcgta aagttcatca ctggtgaaga ggttgagcac cagtttaaag
    19441 tcatccgtca cccagcaacg caccggaata aaaccgccaa agctgtcatg ctcaatctcg
    19501 tagcggttaa attccaccat cacgccgcgt ggctctttca cggcaaggat attttccccc
    19561 ggcagaatct ctggtttttc aatatctgcc agcgccatca ttgtcggcag taaatcaata
    19621 tgactgactg gcgtatcgac ctgtcgccgc tccccttgcg gcgaacggat gatcagcgga
    19681 atacgggtga tgtcgtcata catcgccgct cctttactga tcagcttatg tgcgcccatc
    19741 atttcaccgt gatcggaggt ataaatgacc cacgtatttt cacgttgctc tggcgttaag
    19801 gcattgatga cacgtccgat ttggtcatca acaaagtcat tgcaggcaaa atagagcgga
    19861 tggtgataaa gcccgtcatc accgactggc gatagcatcg cctgcgccca taagcggtga
    19921 tgttccggtt tgttcgccag gtcatcctgt gatttctcgc caagttcgta gtaaaaatca
    19981 gcgtatttct ctaaatactc caccggacag gtgaacgggt gattcggctc atcatacgaa
    20041 accaccatca ggaagggttc ctcggcgcgc acgggctgtt gcagaaaatc taccgctcga
    20101 ttgctgatgc ggtgcgccca ggtgaaggtt tcgtcaatat ggttcgcctg taaatcttcg
    20161 acgctgttta ggccattgcg ccacaggctg atctcttttt ccgtcagttc gctaagatag
    20221 ttcgccccat caaaccagta atcagcatcc cactccggcg gacactcgcc agtgccgaaa
    20281 tagtcatgac cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc ggcatcctta
    20341 aagtagcgcc ccatagtgga gatgtttttg cctggcgcga cgttgttggt ccacgggccg
    20401 gactggttag cgtagatacc ggtgaatagt ccggcgcgtg caggcgtaca aaccggtgaa
    20461 caagtgtagg cggaattaaa gcgaatacct tccgccgcca gactatcaat attttgcgta
    20521 ttcagcggtt taccgctata gcaaccgacc atattggtgg cctgggtatc ggtcaattgg
    20581 ggcgtttcat aggctatcct tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg
    20641 ccgccagctg tcaaaaatca ggtagacaat cactacggca gcgatgaaat aactgatcat
    20701 cgctaaccag cgcgtaccgt agccgccgaa ttcagccaac ccgacataga cgccaatcat
    20761 cgcgaacagg ataccaattg acgcgatctt gacgtttttc cacggtttca tgtccaccgc
    20821 aaacgcatct ttgaaggtga acggcgtggc gcgcgctttg ataaaaccga tcaccagcat
    20881 cacgaccacg ttgatgcaga acgtacaggc cagcacatag aggaaatgga agtcgaactt
    20941 caccagatag ttgatggtga tgtagctgat tatgccaatc cccatcgcca cttttgccgc
    21001 cagcgccggg atgcgcggga agaaaaagcc cataatgatg atggtaacca gcggcacgtt
    21061 gtaaatgccg ttgagctgtt tcatccagct atacagcccc tgcggcgcgt tggcgatcca
    21121 cggcgctacc aaaaccgaaa cgatagcgat aaagaaacca aatttgcgcc cgacggtgac
    21181 cagctgctgc ggctcggcat tctggttaat gatgcgacgg taaataccca tactgaataa
    21241 tgtactggcg ctattcagaa agccgttgaa ggtactgatc accgcaccaa ataacaccgc
    21301 gccgaagaaa cccaccagtg gcactggcag aacgttattg accagcgtcg ggtaggccat
    21361 gtctgctttc ggtagatcct gatacagatg aaacgcaatc aaccctggca gcaccagtac
    21421 cagcgggtcg agcattttca gcaccgccgt taacagcgcc cccttttgcc cttccgccag
    21481 gctttttgac gccagcgtgc gttgcacgat gccctgattt gtacaccagt aaaaggtgtt
    21541 caccagaatt aaaccggtaa atgccgcgcc aatcggcaag ggatcggtcg ggccaccgac
    21601 tgagtttaat ttctcggcgt gaacggtggt aatttgctca atgccctgca taaagctgcc
    21661 cttgcccatc gcgatcaggc caaataccgg caccatcaac ccgccgataa ccagcccaat
    21721 accgttgatg gagtccgcca ctgccattgc gcgcagtccg ccgatcaccg catacaaaat
    21781 tcccgccaga ccaagcaaaa ttaccagtag ccagatagcc gcaccgtgag aaatctgtag
    21841 cgattccccg acgtgaaaca ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg
    21901 cagaaagcag acgccggtgg caatgaggaa gcagaagtcg atgataatac gcgtcgtttt
    21961 atcataacgt tcctccagaa aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg
    22021 taaaaagatt agcgcgagga agatcagcgt caccgctgaa gtcacttccc agcccatcac
    22081 cgacatgccg cttttgtagg cctgcccgga aagaccaacc agttgttccg tggaaaggtt
    22141 ggttaacatt aacgaagcgg caataaccgg cgcttttagt gaacgtccgg caagaaaata
    22201 gccttgttgc gatccggtat ctgttttgcg aaccttccac taggtgatca ccgccaccag
    22261 cagcgtaaaa ccgacaaaac tcaagatttg taacgaattc atctcatagc ccttatttat
    22321 gtttatgtcc cggacgcatg aatataggga gaggtaaaat gtgtcgggtc aggcagtcag
    22381 attcttaaaa tacaaacgtc gtatccctga acggattgtt ttctgttaag ttcaggttgt
    22441 gagcataatt cgccggaggg atattaaaat gaatggaaaa ttgcaaagtt cagatgtaaa
    22501 aaacgaaact ccgtacaata ttcccttatt gattaacgaa aatgtgatct ccagcggaat
    22561 ttctctgatc tcgctctggc atacctacgc cgacgagcat taccgggtga tctggccgcg
    22621 ggacaagaag aaaccgctta tcgccaactc atgggttgcg gtgtataccg tacaaggatg
    22681 tgggaaaatt cttttaaaga atggcgaaca aataactctg catggcaact gtattatatt
    22741 tttaaagcca atggatattc actcttatca ctgtgaaggt ttagtcttgg aacagtactg
    22801 gatggaattt acccccacca gtatgatgga tattcccgtt ggtcagcaaa gcgttattta
    22861 taatggcgaa atttataatc aggaactcac cgaagttgct gagttaataa cttcaccaga
    22921 agcaataaaa aataatctgg cagtcgcttt tctgacgaaa attatttatc agtggatttg
    22981 tcttatgtcc gcagatggta aaaaagatcc acaacggcgg caaattgaaa aattaattgc
    23041 cactttacat gccagtctgc aacaacgctg gagcgtagct gatatggctg ccacgatccc
    23101 ctgtagcgaa gcctggttgc gtcgtctgtt tttacgctat accggcaaga cgccgaaaga
    23161 atattacctc gatgcgcgtc tggatctggc gctatcgcta ttaaagcaac aaggaaactc
    23221 ggttggcgaa gtcgctgata cgctcaactt cttcgactcc tttcatttca gcaaagcctt
    23281 taaacataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
    23341 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
    23401 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc
    23461 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
    23521 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
    23581 aggccgtagt agcgcacgcg catctgtttg cgatcttgca gcccaacaat ttgcgccatc
    23641 cggctttcaa taccgattgg catgtcacag atgttgagga ttttcgcatt cgggcgcaga
    23701 cgacgcgtgg cttctgcgac aattgctgcc gggttggagt agttgagcat ctaggcgttt
    23761 ggtgaatatt tttccatata atccaccagt tccaggacgc cgccaatgga acgcatgccg
    23821 tacgctattc cgccgggtcc gcaagtttcc tgaccaacaa caccgtggcg cagtgggatt
    23881 ttttcatcca gttcgcgcat cgggtacttg ccgacgcgga tgtgcgccat gacaaaatca
    23941 acgtcgctga acgccacttc aggatcggtg gtgtaactaa acgcaatgtc cggcgctttt
    24001 tctttaagga tgactttaca ggcttcggca atcacttcct gccgcgcgcc atcgttatca
    24061 taaaatttca gtgcacgaag cgggaaacgg tcctgattcg ccaggagcat caacacgatg
    24121 cctggcgtaa aggtgcttcc accgcctgcg acaaccactg agaatttggt cattatactg
    24181 cctccgtaat ggcaacattt tctgctgatt gatgagaatt aattaagcta tcgagctgtt
    24241 cacgcaattg ggatacatgc agaccaatga tcacctgaat ggcatcgcca ctacggaaga
    24301 cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag cgtttgtgac atgtcatgca
    24361 gtgcaatacg tagacgcgtc gcgcaattgt taatgctgga gatattgccg accccgccca
    24421 gggcttgcag gataccggca gcctgatcca gctctttttt tggctctgca gcggtggttt
    24481 ggcctcgcga ggctttgtat tcggcttttg agtagagttt cacttccgca tcttcacgtc
    24541 ccggcgtgca catgttgaac tgcagaatca gggtgcggaa aaccacgaag tacagcaggg
    24601 taaagcacaa cccaatggcg atttgggtca gcatcatatc cgcgtggttg ctgaacatcg
    24661 ggatccagtt ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg cccaccacac
    24721 caaagagata cattacggtc gacattgagg ccgccagcac ggcgtgtacc gcaaacagca
    24781 acggtgaaat gaacaggaag gtaaattcca gcggttcgg
//
LOCUS       DADLZM010000051        24461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLZM010000051 DADLZM010000000
VERSION     DADLZM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.051"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..488)
                     /locus_tag="JNY73_002000"
     CDS             complement(<1..488)
                     /locus_tag="JNY73_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY8048024.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKMKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            761..949
                     /locus_tag="JNY73_002001"
     CDS             761..949
                     /locus_tag="JNY73_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048025.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            961..1671
                     /gene="bcsQ"
                     /locus_tag="JNY73_002002"
     CDS             961..1671
                     /gene="bcsQ"
                     /locus_tag="JNY73_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY8048026.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1668..4281
                     /gene="bcsA"
                     /locus_tag="JNY73_002003"
                     /pseudo
     CDS             1668..4281
                     /gene="bcsA"
                     /locus_tag="JNY73_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4292..6631
                     /gene="bcsB"
                     /locus_tag="JNY73_002004"
     CDS             4292..6631
                     /gene="bcsB"
                     /locus_tag="JNY73_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY8048027.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCEKPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNLPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6638..7744
                     /gene="bcsZ"
                     /locus_tag="JNY73_002005"
     CDS             6638..7744
                     /gene="bcsZ"
                     /locus_tag="JNY73_002005"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY8048028.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7803..7979
                     /locus_tag="JNY73_002006"
                     /pseudo
     CDS             7803..7979
                     /locus_tag="JNY73_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8307..10055
                     /gene="bcsC"
                     /locus_tag="JNY73_002007"
                     /pseudo
     CDS             8307..10055
                     /gene="bcsC"
                     /locus_tag="JNY73_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10137..12125
                     /gene="hmsP"
                     /locus_tag="JNY73_002008"
     CDS             10137..12125
                     /gene="hmsP"
                     /locus_tag="JNY73_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY8048029.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12309..13595
                     /locus_tag="JNY73_002009"
     CDS             12309..13595
                     /locus_tag="JNY73_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY8048030.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13816..15312
                     /locus_tag="JNY73_002010"
     CDS             13816..15312
                     /locus_tag="JNY73_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY8048031.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15408..16556)
                     /locus_tag="JNY73_002011"
     CDS             complement(15408..16556)
                     /locus_tag="JNY73_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8048032.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKDADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGAGSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSVENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16569..17336
                     /gene="pdeH"
                     /locus_tag="JNY73_002012"
     CDS             16569..17336
                     /gene="pdeH"
                     /locus_tag="JNY73_002012"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY8048033.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17406..19466
                     /locus_tag="JNY73_002013"
     CDS             17406..19466
                     /locus_tag="JNY73_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY8048034.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19648..20970)
                     /locus_tag="JNY73_002014"
     CDS             complement(19648..20970)
                     /locus_tag="JNY73_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY8048035.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21381..22394)
                     /gene="yhjD"
                     /locus_tag="JNY73_002015"
     CDS             complement(21381..22394)
                     /gene="yhjD"
                     /locus_tag="JNY73_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY8048036.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22443..23414)
                     /locus_tag="JNY73_002016"
     CDS             complement(22443..23414)
                     /locus_tag="JNY73_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8048037.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            23862..>24461
                     /locus_tag="JNY73_002017"
     CDS             23862..>24461
                     /locus_tag="JNY73_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8048038.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5791 a         6165 c         6730 g         5775 t
ORIGIN      
        1 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
       61 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
      121 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
      181 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
      241 aataatttca ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
      301 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
      361 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
      421 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
      481 tccctcatgt caccattttg tctgattatc agtttactag cgaaagctaa gaaataaacc
      541 taccattgaa attaaaactt atcagattta gcatgtgaat caatttagaa ggtcacaaga
      601 cgtgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa agtcaaaaag
      661 tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg gttagaattg
      721 tatcatgaat ggtacggtta tttcataggg atcaagcaaa atgaataaca atgaaccaga
      781 tactctgcct gatcccgcga taggctatat cttccagaat gatattgtgg cgttaaagca
      841 ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac agttggccgc
      901 ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg ggattggtga
      961 atggccgtac tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac catcaccgcc
     1021 gcattagcct ggtcattaca aatgttggga gaaaatgtcc tagtggtcga tgcctgcccg
     1081 gacaacttgt tgcgcctgtc atttaatgtt gattttaccc accgtcaggg ctgggccaga
     1141 gcgatgctgg atggccagga ctggcgtgac gctgggttgc gctacacctc gcagctcgat
     1201 ttgctgcctt ttggtcagtt atccattgaa gaacaagaaa atccacagca ctggcaaacc
     1261 cggctgagcg atatttgctc cggcttacag caactaaaag ccagcgggcg ttaccagtgg
     1321 attttaatcg acttaccgcg tgatgcctcg cagataaccc accagctgct gagtttgtgc
     1381 gagcactcgc tggcaatcgt caatgtggat gccaactgcc atatccgact gcatcagcaa
     1441 gcgctgccgg atggcgcaca tattttgatt aatgacttcc gtattggcag tcaggttcag
     1501 gacgatattt accagctttg gttgcaaagc cagcgccgat tactgccgat gctcattcat
     1561 cgtgatgaag cgatggctga atgcctggcg gctgaagaga tactgacgct ggcgaactgg
     1621 tgcctgttga actactccgg gctgaaaacg ccagtcggga gtgcatcatg agtatcctga
     1681 cccggtggtt gcttatcccg ccggtcaacg cgcggcttat cgggcgttat cgcgattatc
     1741 gtcgtcacgg tgcgtcggct ttcagcgcga cgctcggctg tttctggatg atcctggcct
     1801 ggatttttat tccgctggag cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc
     1861 tgtatacgcc aacgcctcgc gtccgcgtcc gctggacccg gtccgttatc tcattcaaac
     1921 atgctggtta ttgatcggtg catcgcgcaa agaaacgccg aaaccgcgca gcgggcattt
     1981 tcaggtctgc aaaatattcg tggacgttac catcaatgga tgaacgagct gcctgagcgc
     2041 gttagccata aaacacagca tctggatgaa aaaaaagagc tcggtcattt gagtgccggg
     2101 gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc tgattctggc gttaatctgc
     2161 gttactcagc cgtttaaccc gctggcgcag tttatcttcc tgatgctgct gtggggggta
     2221 gcgctgatcg tacggcggat gccggggcgc ttctcggcgc taatgttgat tgtgctgtcg
     2281 ctgaccgttt cttgccgtta tatctggtgg cgttacacct ctacgctgaa ctgggacgat
     2341 ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg aaacgtacgc gtggattgtg
     2401 ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc gtcagccggt gccattgccg
     2461 aaggatatgt cgctgtggcc gtcagtggat atctttgtcc cgacttacaa cgaagatctc
     2521 aatgtggtga aaaataccat ttacgcctcg ctgggtatcg actggccgaa agacaaactg
     2581 aacatctgga tcctcgatga cggcggcagg gaagagtttc gccagtttgc gcaaaacgtg
     2641 ggggtgaaat atctcgcccg caccactcat gaacatgcga aagcgggcaa catcaacaat
     2701 gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg actgcgacca cgtaccaacg
     2761 cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag agaaacagct ggcgatgatg
     2821 cagactccac accacttctt ctcgccggac ccgtttgaac gcaacctggg acgttttcgt
     2881 aaaacgccga acgaaggcac gctgttctat ggtctggtgc aggatggcaa cgatatgtgg
     2941 gacgccactt tcttctgcgg ttcctgtgcg gtgattcgtc gtaagccgct ggatgaaatt
     3001 ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata cttctctgcg tttgcaccgt
     3061 cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg cggggctggc gaccgaaagt
     3121 ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg ggatggtaca aatcttccgt
     3181 ctcgataacc cgctcaccgg taaaggactg aagtttgctc agcggctgtg ctacgtcaac
     3241 gccatgttcc acttcttgtc gggcattcca cggctgatct tcctgactgc gccgctggcg
     3301 ttcctgctgc ttcatgccta catcatctat gcgccagcgt tgatgatcgc cctattcgtg
     3361 ctgccgcata tgatccatgc cagcctgacc aactcgaaga tccagggcaa atatcgccac
     3421 tctttctgga gtgaaatcta cgaaacggtg ctggcgtggt atatcgcacc accgacgctg
     3481 gtggcgctga ttaacccgca caaaggcaaa tttaacgtca ccgccaaagg tggactggtg
     3541 gaagaagagt acgtcgactg ggtgatctcg cggccctaca tcttccttgt tctactcaac
     3601 ctggtgggcg ttgcggtagg catctggcgc tacttctatg gcccgccaac cgagatgctc
     3661 accgtggtcg tcagtatggt gtgggtgttc tacaacctga ttgttcttag cggcgcagtt
     3721 gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc gcgtggagat gacgatgccc
     3781 gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta ccgttcagga tttctccgac
     3841 ggtggtttgg ggatcaagat caacggtcag gcgcagattc tggaagggca gaaagtgaat
     3901 ctgttgctta aacgcggtca gcaggaatac gtcttcccga cccaggtggc gcgcgtgatg
     3961 ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc agcaacatat cgattttgtg
     4021 cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc aggacagcta cccggaagat
     4081 aagccgctgg aaagtctgct ggatattctg aagctcggct tccgtggcta ccgccatctg
     4141 gcggagtttg cgccttcttc ggtgaagggc atattccgtg tgctgacttc tctggtttcc
     4201 tgggttgtat cgtttattcc gcgccgcccg gagcggagcg aaacggcaca accatcggat
     4261 caggctttgg ctcaacaatg atgataacgc gatgaaaaga aaactattct ggatttgtgc
     4321 agtggctatg gggatgagtg cgttcccctc tttcatgacg caggcgacgc cagcaacgca
     4381 accactgatc aatgctgagc cagctgtagc cgcccagacg gaacaaaatc cgcaggtggg
     4441 gcaagtgatg tcaggcgagc agggagctga tgcccccatc gtggcgcaga acggtccttc
     4501 gcgtgatgtg aagctgacct ttgcgcaaat cgcgccgccg ccgggcagca tggtgctacg
     4561 tggtattaac ccgaacggca gcattgagtt tggtatgcgc agcgatgaag tggtgacgaa
     4621 ggcgatgctc aacctcgaat acacgccatc gccatcgtta ctgcctgtcc agtcgcagtt
     4681 aaaggtttat ctcaatgatg agctgatggg cgtgctgcca gtgaccaaag aacagttggg
     4741 taaaaaaacg ctggcgcaaa tgcccattaa tccactgttt attaccgact tcaaccgtgt
     4801 gcggctggag tttgtcggcc attatcagga cgtgtgcgaa aaaccggcca gcaccacgct
     4861 ttggctggat gttgggcgga gcagtggact ggatctgacc tatcagaccc tgaatgtgaa
     4921 gaatgacctg tcacacttcc cggtgccatt ctttgacccg cgcgataacc gtactaacac
     4981 cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg caacaagcat ctgccattgt
     5041 cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag aacctcccgg tgctctataa
     5101 ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac gacaaacggc cggacttcct
     5161 gcgcgatcat ccggcggtaa aagccccggt gattgagatg attaaccatc cgcagaatcc
     5221 ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa gacctgttgc aggcagcgaa
     5281 aggtatcgct cagggtaaca ttctgttccg tggtgaaagc gtggtagtga atgaagtgaa
     5341 accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg gtacgtaccg atcgtccggt
     5401 cacatttggc gaactgaaaa cctatgaaga acagttacaa tccagcggcc ttgagccagc
     5461 agcgattaac gtttcgctaa acctgccgcc ggatctctac ctgatgcgca gtaccggcat
     5521 tgatatggat attaattacc gctacaccat gccgccggtg aaagacagtt cgcggatgga
     5581 tatcagcctg aataaccagt tcctgcaatc cttcaacctg agcagcaaac aggaggcgaa
     5641 ccgtctgctg ctgcgtattc cggtattaca aggtttgctg gatggcaaaa cagatgtctc
     5701 tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc ttcgactttg agtatatgaa
     5761 cccgatgcca ggcggttcgg tagataactg tattaccttc cagccggtgc agaatcatgt
     5821 ggtgattggt gacgattcca ccatcgactt ctcgaagtat taccacttca tcccgatgcc
     5881 ggatctacgc gcctttgcta acgcgggctt cccattcagc cggatggcgg atctgtcgca
     5941 aaccatcacc gtgatgccga aagcgcctaa cgaagcacag atggaaacgt tgctgaatac
     6001 tgttggtttt atcggcgcac agacgggctt cccggcgatt aatctgacgg tgaccgatga
     6061 tggcagcacc attcagggca aagatgccga catcatgatc atcggtggta tcccggacaa
     6121 actgaaagac gataagcaga tcgacctatt ggtgcaggcg accgaaagct gggtgaaaac
     6181 accgatgcgc cagaccccgt tccccggcat tgtgccggac gagagcgatc gcgcggcaga
     6241 aacccagtca acgctgacct cttccggtgc gatggcggcg gtgattggct tccagtcgcc
     6301 gtataacgac cagcgcagcg tgattgcgct gttggcagat agcccacgcg gttatgaaat
     6361 gcttaacgat gcggtgaacg atagcggcaa acgcgccacc atgttcggtt cggtcgcggt
     6421 gatccgcgag tacggtatca acagcctacg tgttggcgac gtttattacg taggtcatct
     6481 gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat ccgattctgc tggcggtgct
     6541 ggcggctatc agtgtgatat tgctggcatg ggtactgtgg cgtctgctgc gaattattag
     6601 tcgtcgtcgt cttaacccgg ataacgagta attgaagatg aatgtgttgc gtagtggaat
     6661 cgtgacgatg ctgttgttgg ctgcctttag tgttcaggca gcctgtacct ggcctgcctg
     6721 ggagcagttt aaaaaggatt acatcagtca ggaagggcgc gtcatcgacc ccagcgacgc
     6781 gcgcaaaatc accacctccg aagggcaaag ttacggtatg ttctttgccc tggcggctaa
     6841 cgaccgtgca gctttcgata atattctcga ctggacgcag aacaatctcg ctcagggttc
     6901 tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa gagaacagta agtgggaagt
     6961 gctggacagc aattcggcct ccgatggtga tgtctggatg gcctggtcgt tgctggaggc
     7021 ggggcgtttg tggaaagagc agcgttatac cgacatcggc agcgcgttgc taaaacgtat
     7081 cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc atgttgttac cgggcaaagt
     7141 gggttttgct gaggataaca gctggcgttt taaccccagc tacatgccgc cgacgctggc
     7201 gcagtatttc acccgctttg gcgcgccgtg gaccacgctg cgcgaaacca atcaacattt
     7261 attgctggaa accgccccga aaggcttttc gccagactgg gtgcgctatg agaaagacaa
     7321 aggctggcag ctaaaagccg aaaaaacatt gatcagcagc tacgacgcta tccgcgttta
     7381 catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa gcgcggatgc tcaaccggtt
     7441 taaaccgatg gcgacattca ctgagaaaaa cggttatccg ccggaaaaag tggatgtggc
     7501 tacggggaaa gcgcagggta aaggaccggt cggtttttct gccgccatgc tgcctttttt
     7561 acaaaaccgc gatgcgcagg cagttcagcg ccagcgcgtg gccgataact ttcccggcag
     7621 cgatacctat tacaactatg tgctgaccct gtttggacaa ggctgggatc aacaccgttt
     7681 ccgcttctcg acaaaaggtg agttattacc tgactggggc caggaatgcg caaattcaca
     7741 ctaaacatat tcacgctttc cctcggtctg gccgtcatgc cgatggtcga ggcagcacca
     7801 accatcagca gaccctgcgg atggacagcg gcaacactaa tgccgtgcgc gggctggcga
     7861 atatttaccg ccagcagtcg ccagaaaaag ctgaagcgtt tatcgcctcg ctctctgcca
     7921 gccagcggcg cagcattgat gatatcgaac gcagcctgca aaacgaccgt ctggcacagc
     7981 aggcagaggt actggaaaac cagggcaaat gggcgcaggc ggcagcactt cagcggcaac
     8041 gactggcgct ggaccccggc agcgtatgga ttacttaccg actttcgcag gatctctggc
     8101 aggccggaca acgcagccag gccgatacgt taatgcgcaa tctggcgcag cagaagccga
     8161 acaacccgga gcaggtttac gcttacgggc tgtacctctc aggtcacaac caggacagag
     8221 cggcgctggc gcatatcaac agcctgccgc gcgcgcagta gaacagcaat attcaggagc
     8281 tggttaatcg actgcaaagc gatcaggtgc tggaaaccgc taatcgcctg cgagaaagcg
     8341 gcaaagaggc tgaagcggaa gcgatgctgc gtcagcaacc accttccacg cgtattgacc
     8401 tcacgctggc tgactgggcg taacaacgac gtgattacac cgccgcccgc actgcatatc
     8461 agaatgtcct gacgcgggag ccaactaacg ccgatgccat tcttggtctg acggaagtgg
     8521 atattgctgc cggtgacaaa gcggcggcac gtagccagct ggcgaaactg cctgccaccg
     8581 ataacacctc gctgaacact cagcggcgcg tggcgctggc acaggcgcag cttggagata
     8641 ccgcagcggc gcagcagacg tttaataagt tgatcccgca ggcaaaatct cagccaccgt
     8701 cgatggaaag cgcgatggtg ctgcgcgacg gtgcgaagtt tgaagcgcag gcgggcgatc
     8761 caaagcaggc gctggaaacc tacaaagacg ccatggtcgc atccggtgtg actacgacgc
     8821 gtccgcagga taacgacacc tttacccgac tgacccgtaa cgacgagaaa gatgactggc
     8881 tgaaacgcgg cgtgcgcagc gatgcggcgg acctctatcg ccagcaggat cttaacgtca
     8941 cccttgagca cgattactgg ggttcgagcg gcaccggtgg ttactccgat ctgaaagcgc
     9001 acactaccat gttgcaggtg gatgcgccgt attctgacgg gcggatgttc tttcgcagtg
     9061 atttcgtcaa tatgaacgtc ggcagtttct ccactaatgc cgatggcaaa tgggatgaca
     9121 actggggcac ctgtacatta caggactgta gcggcaaccg cagccagtcg gattccggtg
     9181 ccagcgtggc ggtcggctgg cgaaatgacg tttggagctg ggatatcggt accacgccga
     9241 tgggcttcaa cgtggtggat gtggtcggcg gcatcagtta cagcgatgat atcgggccgc
     9301 tgggttacac cgttaacgcc caccgtcggc ccatctccag ttctttgctg gcctttggtg
     9361 ggcaaaaaga ctccccgagc aataccggga aaaaatgggg tggcgtacgt gccgacggtg
     9421 tggggctaag tctgagctac gataaaggtg aagtaaacgg cgtctgggca tcgcttagtg
     9481 gcgaccagtt aaccggtaaa aatgtcgaag ataactggcg cgtgcgctgg atgacgggct
     9541 attactataa ggtcattaac cagaacaatc gccgcgtcac aatcggcctg aacaacatga
     9601 tctggcatta cgacaaagat ctgagtggct actcactcgg tcagggcggt tactacagtc
     9661 cgcaggaata cctgtcgttt gccataccgg tgatgtggcg ggagcgcacg gaaaactggt
     9721 cgtgggagct gggggcgtct ggctcgtggt cgcattcacg caccaaaacc atgccgcgtt
     9781 atccgctgat gaatctgatc ccgaccgact ggcaggaaga agctgcgcgg caatccaacg
     9841 atggcggcag cagtcagggc ttcggctaca cggcgcgggc attacttgaa cgacgtgtta
     9901 cttccaactg gtttgttggc acggcaattg atatccagca ggcgaaagat tacgcaccca
     9961 gccatttcct gctctacgta cgttattccg ccgccggatg gcagggtgac atggatttac
    10021 cgccgcagcc gctgatacct tacgccgact ggtaagtttt cagatagcgc ctctcttaat
    10081 gccgctgcga tcgggtatac tcgggcggca atctgggatt tccgggggga gacaatttgc
    10141 gcgtaagtcg ctcgttaaca atcaagcaga tggcaatggt ggcagccgtt gtcctggtgt
    10201 tcgtttttat tttttgcacc gttttgctgt tccatctggt ccagcagaat cgctataaca
    10261 cggctacgca actggaaagc attgctcgct ctgtccgcga acccttatct tcagctattt
    10321 tgaaaggcga tattcccgaa gcggaagcta ttcttgccag cattaaaccg gcaggcgtgg
    10381 tcagccgtgc cgatgtagtg ctgcctaacc agttccaggc gctgcgtaaa agttttattc
    10441 cagagcgtcc ggtgccggta atggttactc gcctgtttga gctaccggtt caaatctcgc
    10501 tgggcgtcta ctcgctcgaa cgtccggcaa atccgcagcc aatagcctat ctggtgctac
    10561 aggcggattc cttccgtatg tataagttcg tgatgagcac tctctcaacg ttagtgacca
    10621 tttacttact tttgtcgctt atcctgaccg tcgccatcag ctggtgcatt aaccgcctga
    10681 ttttgcatcc gttacgcaat attgctcgcg aacttaacgc catcccagcc caggagcttg
    10741 ttggtcacca actggcatta ccgcgtctgc atcaggacga tgaaatcggt atgttggtgc
    10801 gcagttacaa cctcaaccag caattgctgc agcgccatta tgaagaacag aacgaaaatg
    10861 cgatgcgctt cccggtgtcg gatttgccga acaaagcctt gctgatggag atgctggagc
    10921 aggttgtcgc gcgtaaacaa accaccgcgc tgatgatcat cacctgtgaa accctgcgtg
    10981 atactgcggg cgtgctgaaa gaggcgcaac gagaaattct gctgctgacg ctggtggaaa
    11041 aactcaaatc ggtactgtcg ccacgtatga tcctcgcgca gattagcggt tatgactttg
    11101 ctgtcattgc caacggtgta caggaaccgt ggcacgcaat cactttgggt cagcaagtac
    11161 tcactatcat gagcgagcgc ctgccgattg aacgtattca actccgtccg cactgtagca
    11221 ttggcgtggc gatgttctac ggcgatctca ccgccgaaca gctttacagt cgcgctattt
    11281 ctgcggcatt taccgctcgc cataaaggca agaatcagat tcagttcttt gatccgcagc
    11341 agatggaagc agcccagaag cggttgacgg aagagagcga tatccttaat gcactggaaa
    11401 atcatcagtt tgcaatttgg ttacagccac aggtcgagat gaccagcggt aaactggtca
    11461 gtgcggaagt gttactgcgt atccagcaac cggatggcag ttgggacctg ccggatggct
    11521 taatcgatcg cattgagtgc tgtgggctga tggttaccgt cggtcactgg gtgctggaag
    11581 agtcctgtcg attgcttgca gcctggcaag agcgcggcat tatgctgccc ttgtcggtaa
    11641 acctctctgc gctgcaactg atgcacccga atatggtggc ggatatgctg gaactgttaa
    11701 cccgctatcg cattcagccg ggaacactga ttctggaagt gacagaaagc cgacgtattg
    11761 acgaccctca tgctgcggtg gcaatcctcc gtccgctgcg caatgccgga gttcgggtgg
    11821 cgctggatga tttcggtatg ggctacgcag ggctgcgtca gctgcagcat atgaaatcgt
    11881 tgccaatcga cgtactgaaa atcgacaaaa tgtttgttga aggcttgccg gaagatagca
    11941 gcatgattgc tgcaattatc atgctggcgc agagcctgaa cttacaaatg attgccgaag
    12001 gcgtggagac tgaagcacaa cgagactggc tggcaaaagc gggcgttggt attgcccagg
    12061 gcttcctttt tgctcgccca ctccctattg aaatcttcga agagagttac ctggaagaaa
    12121 agtagctacc ccaaactgat tacaaaactt taaaaagtgc tggtttgtgc gagccagctc
    12181 aaacttttta acctttttgt ttcaattatg atccaggtac atttctgtga tgttgtctgg
    12241 gtgttatttt aaggccgcag gtacccccat aaccttacaa gacctgtggt tttactaaag
    12301 gacaccctat gaaaacctct ctgtttaaaa gcctttactt tcaggtcctg acagcgatag
    12361 ccattggtat tctccttggc catttctatc ctgaaatagg cgagcaaatg aaaccgcttg
    12421 gcgacggctt cgttaagctc attaagatga tcatcgctcc tgtcatcttt tgtaccgtcg
    12481 taacgggcat tgcgggcatg gaaagcatga aggcggtcgg tcgtaccggc gcagtcgcac
    12541 tgctttactt tgaaattgtc agtaccatcg cgctgattat tggtcttatc atcgttaacg
    12601 tcgtgcagcc tggtgccgga atgaacgtcg atccggcaac gcttgatgcg aaagcggtag
    12661 cggtttacgc cgatcaggcg aaagaccagg gcattgtcgc cttcattatg gatgtcatcc
    12721 cggcgagcgt cattggcgca tttgccagcg gtaacattct gcaggtgctg ctgtttgccg
    12781 tactgtttgg ttttgcgctc caccgtctgg gcagcaaagg ccaactgatt tttaacgtga
    12841 tcgaaagttt ctcgcaggtc atcttcggca tcatcaatat gatcatgcgt ctggcaccta
    12901 ttggtgcgtt cggggcaatg gcgtttacca tcggtaaata cggcgtcggc acactggtgc
    12961 aactggggca gctgattatc tgtttctaca ttacctgtat cctgtttgtg gtgctggtat
    13021 tgggttcaat cgctaaagcg actggtttca gtatcttcaa atttatccgc tacatccgtg
    13081 aagaactgct gattgtactg gggacttcat cttccgagtc ggcgctgccg cgtatgctcg
    13141 acaagatgga gaaactcggc tgccgtaaat cggtggtggg gctggtcatc ccgacaggct
    13201 actcgtttaa ccttgatggc acatcgatat acctgacaat ggcggcggtg tttatcgccc
    13261 aggccactaa cagtcagatg gatatcgtcc accaaatcac gctgttaatc gtgttgctgc
    13321 tttcttctaa aggggcggca ggggtaacgg gtagtggctt tatcgtgctg gcggcgacgc
    13381 tttctgcggt gggccatttg ccggtagcgg gtctggcgct gatcctcggt atcgaccgct
    13441 ttatgtcaga agctcgtgcg ctaactaacc tggtcggtaa cggcgtagcg accattgtcg
    13501 ttgctaagtg ggtgaaagaa ctggaccaca aaaaactgga cgatgtgctg aataatcgtg
    13561 cgccggatgg caaaacgcac gaattatcct cttaatccca ccacttatgc ccgcactccc
    13621 ttccccgagt gcgggtgttt gcgcataatt gacccctgtg cctgaactta ccacctgggt
    13681 gagttcaggt ttatttcact tcttccgtga cattttcatg ttcttgcggt ctaacacgaa
    13741 gtgtttttaa cgtcatttag actgaccgac aacggacagt ttttttatta ccaggatagt
    13801 tgatcagggg ttcacatgca gggcataaaa attcgacttt tagcgggcgg tttgctgatg
    13861 atggccactg ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag
    13921 gggacgcttt ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat
    13981 cgtgttgaaa ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc
    14041 ggttacagtc acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca
    14101 caggcgcgtt cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt
    14161 gtctcttatg acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg
    14221 aaagaagcgc tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc
    14281 atcaaccacg cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag
    14341 ggctggtggc gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg
    14401 ctgaaacaac ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg
    14461 gatgcaatga cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc
    14521 aacaaaacgt ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt
    14581 tctccgctgc gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta
    14641 tctatcatgt gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac
    14701 tggcgtgcgg acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc
    14761 agtgacagca aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag
    14821 tgtgccatca acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg
    14881 cgtgaactgg cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg
    14941 gcgcaaaaga aactggagct gcagaaactg tttgccgcct atgcacgggc tgataccgat
    15001 attctgatgg gccagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag
    15061 cagtatcaga aactgcggca ggatttcctt aacagcctga ctgtggagat gttaaatcag
    15121 gatctgcgtc agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag
    15181 ccggaattta acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatctacc
    15241 gccgctgcga ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca
    15301 cctgcacagt aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag
    15361 ctggatagag gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc
    15421 gcctcacgcg ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg
    15481 cgtttcgccg cgttttccac actgccgccc gtcaaacgta ccgccagata accggcgctg
    15541 aaagagtcgc cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga
    15601 acatcaacta acgcttcacc agcaatggac accaggcaag aacccgcccc gcgtttcacc
    15661 accacttctt tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga
    15721 ccccacagcg cgtcttcgtc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt
    15781 tgctggtaca cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg
    15841 tcgaaaatca cttttccgcc tttggcgcgg cattcgcgca gcagggaaag cagcttttcg
    15901 cggctggtcg ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc
    15961 gccagctctt cgcaaatcgc cgcagactgc tcgctcgcca gccagaattt ggcggcggct
    16021 tcgttacgcc agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat gtagtaaagg
    16081 cccggaagac ggttttccat ccgttgggtc agggaagtat caacgttctc gccgtgccag
    16141 gcgtccagca tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt
    16201 aatgccgcag gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg
    16261 aaaccgcgct taacgtccgc gtctttctcg gaaagctcaa tcatgcattc gccaatcacg
    16321 gcaatctttt tagacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct
    16381 gtggtggttt gctggtcaac catattaaaa cagtgttcca ttattttttt gagccagaac
    16441 gtattcctga aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt
    16501 ctgcccttac gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga
    16561 catctttgat gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga
    16621 gcttgcagga acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct
    16681 atcaaacatg cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga
    16741 acccttcgca acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga
    16801 tggaggttgt gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc
    16861 acggcctgct ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac
    16921 caaaaatcct gcgccagatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata
    16981 tccgtctgcc gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg
    17041 atgattttgg taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca
    17101 tcaaaatcgc gcgagaactg tttgtgatgc tgcgtcagtc gccggaaggc cgcacactct
    17161 tttctcagct tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag
    17221 aaacaccgga agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt
    17281 ttctttcacg cccggcaccg atggaaacgc tgaatacggc ggttctggcg ctataagctg
    17341 cctcattttc cgccgggctg gactatcttt aggactggca caggaagaat atgaggaagc
    17401 gaacgatgag caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga
    17461 ttgtcgtggc gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca
    17521 accagaaagt ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg
    17581 tgtgggagcg gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac
    17641 atgcagaaga catcattctt ggtaatccac cggatattcc cgaagtcacg atggtgcatt
    17701 tgccgcgagt agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc
    17761 cgtggatcaa gctcgaaaag cccgacgcgc gcctgattcg cctctctgaa aagaacaata
    17821 actggacgtt taatcttgcc aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat
    17881 ggtcgtttcg gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag
    17941 taagcaaagc ggatctggaa atttttgttg atccgttagg caagccgctg ccattcagcg
    18001 aagttactgg atcgaaaggt aaagcggata aagaaaaggt gggcgattac atttttggcc
    18061 tgaaggcgca gggacgttat aacggtgaac cgctcacggg tacgggaaaa ataggcggta
    18121 tgctggcgct gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc cgttcaggta
    18181 atacccgtgt tgcttttgat ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt
    18241 tacggcttaa attttctggc gattcactgg gtgatctcta tgaactgacg ggcgttctgc
    18301 tgcccgatac cccgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa
    18361 aatcgtcggt ctttgattat cgcggtttta atgggcgaat tggcgatagc gatatccacg
    18421 gttctctggt ctacaccacc ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc
    18481 ggcaattgcg gctggcggac ctgggaccgt tgattggcgt tgattccggg aaaggggcag
    18541 aaaagtcgaa acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag
    18601 tgctgcctta tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct
    18661 tcaaagggcg gcgcattgag catggcagta gcctgccgat tagcgatctt tctactcata
    18721 tcatcctcaa aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca
    18781 gcattgcggc gaatattcat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata
    18841 ttcaggctcg tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg atgcagaaga
    18901 cgctggggga aatgaacggt gacgcggaac tacgcggtag cggtaactcg gtggcggcgc
    18961 ttttaggcaa cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca
    19021 acctgatgga gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg
    19081 acgatgaggt gcgggtgaac tgcgcggcgg cgaatctgca tattgccaac ggcgtggcgc
    19141 gcccgcagat ttttgctttc gatactgaga acgcgttgat taatgttacc ggcacggcaa
    19201 gttttgcttc ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta
    19261 tcacactgcg ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga
    19321 aagccggacc gctgattgcc cgtggtgctg ttgctgcggc actggcaacg ctggtaacac
    19381 cggcggcggc gttactggca ctgatctcac cttccgaagg ggaggctaat cagtgtcgga
    19441 cgatattgtc gcagatgaag aagtgatgat aattgccgga tgtctgacat ccggcaatta
    19501 aacagattta gatttttggc aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct
    19561 tatccggcct acaaatcctt gtgaattcaa tatattgtat ggaaattcag tcctgataag
    19621 cgtagcgcat caggcttttc acacttatta caacgactga tgtcgcgtct catgggtcag
    19681 cagcagggcg attaacgtca agccagccat cgccgccaga tataacccca ccgcacctaa
    19741 cccgtagtta gcctgcaacc aagctgcgat atacggcgca acggaagccc cgagaatcga
    19801 cgatacgttg taagagaacg atgctccggt gtaacgcact tctgtcggaa acagctctgg
    19861 taacagcgca cccatcggcc cgaaggtcag ccccatcaga cttaacccca gcagcaggaa
    19921 ggcaaaaacc agaatcgggt tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa
    19981 caggatgatc agcgtggtga tgatcaccat gcttttacgg cgaccaaagg catcagccag
    20041 taatccggcg actggcacca tcacgccaaa accaataact gccatcatca gcatccacaa
    20101 cacttcgtta cgcggcaggc caagcccaac tggcgcggcg gcggtactga aggtcataga
    20161 gtagaccgtc atgatgtaaa acagcgtata ggttgccagc ataatgaacg tacccagtac
    20221 ggttacgcga acatgtttgg tcagcagcgt acccagcggg atcttcacct gttttttcgc
    20281 tttagcgact ttctcaaaca ccggcgactc atgcagcgac acgcgaacat acaggccgat
    20341 aatgaccagc accgccgaga agataaatgg cacacgccag ccccagctca taaactgctc
    20401 atcggtcagt agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg
    20461 tgcgcctagc tgtgggaagg agccatacag tgcacgtttg cgcggtgggg cgttttcagt
    20521 cgccagcagc gccgcgccgc cccattcacc gcctaagccc agaccctgac caaatcgagc
    20581 cagcgccagc agcagtgggg cgaaaatacc aatcgtggca tagcccggca gcagaccaat
    20641 caccacggtc gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac
    20701 gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga aggcgatggc
    20761 gaaggtggcg agcgactgta gcgttgctgc cgttggatcg ccctgcggga agaagatatg
    20821 cggaaacaca ataacggccg cagtggcgta aatgtaaaag tcgaagaact caatggccgt
    20881 gccaatgaga gaggcgacaa ggactttatt acgcgagttg atcggcgtgt attcttgttc
    20941 gtggtcgagt gttgtggctg ttgcttgcat aatcttttct tattttttgg ctaacgaata
    21001 gccatattac gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa
    21061 aaaccaggca aaaaggggat aatgtttagg ccagtgattt ttcttctatg aaatgcttca
    21121 cataatttta taaatagtgg ataaaactgg tttcaggtta aataaaagtt accaactgga
    21181 tttatatgct ggggttatga gttggagtga aattgtttgc tcccaaatag atggctgaag
    21241 aggctctggg gaagagtttc aagaggttat gtgcgcatta tagccggatg cgatgctggc
    21301 gcatcttatc cggcctacgg ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt
    21361 tacgccgcat ccggcctatt ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta
    21421 tattcggcgg tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa
    21481 aatgccatca gccccagcac ggaaccaaat gctgcgccag agggggattt catcagcgat
    21541 ggcagggtgt aggtcatcac gattttaatc acttcaaaac caatagcagc cagaaatgtt
    21601 ccgcgaatca gggctttttt gcgcggacgg tggcgcggca accgccagaa gatccagaag
    21661 aaaagcagat agttggcgaa gatggaaatc gccaggccaa tcaatcgcca cgtcggtttc
    21721 agccactcaa tgctgttcag gtgcagggcg ctaataatca tttgctgcgc cgaaccggca
    21781 accgaggtga tcgaaagcgt cacaatcagc gcaatcaaca aaccaatcag tgaaataaaa
    21841 tcacgcagat attttaccca gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg
    21901 cgcgactggg cacgaatcgc ttcacgcagg ttacccatcc agttgatgcc ggaataaagt
    21961 gccaccgcca ggccgacaag ccctacagtc gtacgctgct gaacggcggt gttgatggtg
    22021 tttttcaacg tggcggctag tgtcggatcg ctgatgtttt gcagaatttt gtcgaagata
    22081 tcctgtagca acatcggatg ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc
    22141 aaaatcggga tcatcgacaa aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc
    22201 agccgatcgt taaagcgttc tgtcgcgcga ataaggtgcg caataaccgg ttggcgttgg
    22261 actttttcgg cagtggtggt gacggtttcc agcgcctggc taactttgcc gcctttctcg
    22321 ctattatcca actgcttaat cggctcgtgc tccagatcct gggtgggacg tttgatctcg
    22381 ttttcctgcg tcatccgctc ttttgtcctt ctgtcgtggt cattcttaaa aagtatagtc
    22441 ggtcagtcca ggtgctcttt cattacgcca gccagccact gcataaacag gtttacacgc
    22501 cgggaaagct cccgacgctg cgggtaaacc agcgaaaggg agagcggctc ggcacggtag
    22561 cctggcaaca cttcaataag ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg
    22621 atctgaataa tccccagacc ggcaagaccg gcggcgagat agttttcgct actgtttacc
    22681 gtcaacatgc cgccagactt aaaccactgg acgccattaa cgctggcaac ctcaaaacct
    22741 aacggatgta cacccaggtg cggtgtataa cgcactattg cgtgtgaagt cagatcatcg
    22801 gggctttgcg gataaccaaa gcgcgtcaga tagtgcggac tggcacagtt gaccatggtt
    22861 agtttgccga ggggacgggc gataacgcca tcttccggta acgcgccagt gcgtatcacg
    22921 caatcaaaac catcatgaag aatatctacc ggacggttat ggctactcag ttccagctca
    22981 attcccggat gctgatagag aaattccgac agacgcggca gtaacaggtt tttcgcgatt
    23041 cctgacggga tgtcgatgcg taatttaccg ctgatactgg tggcatcctg ttgaaacaga
    23101 ccgtccagtt cgctgagatt actcaacaca tcttttgccc gttgataata ggtcatgcct
    23161 tctggcgtga gtttgacccg tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt
    23221 tccagtgcct gtatctggcg cgaaacactt ccctttggca aagcaaagaa atccgccgcg
    23281 cgggaaaaac tttccagctc cgcgactttg atgaacaact gcattgcgtg aattttatcc
    23341 atttctttga ccgattgttg ttcacagcga aacagtaacg cgcatatcgc attgtttatt
    23401 gttttttcta acatctaatg ccgggtttca caatgttgtc atgacgaatg tggcgatttc
    23461 tcagcaggaa aagggcggaa tagctttatg agaaaggtga atccccttaa acagagcgta
    23521 tttttcagtg attcccttta aagtgaatag aggacgtatt ggctatttgt gcttattttt
    23581 gtctcattgc catttgttat tttggcttgt agtcgcggaa tttcattttt aattgcatgc
    23641 gtactaacca ccaatataag gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt
    23701 aatttcaggc gtttagctgt aactatttat atcggtaact attttctacg cgtcatctct
    23761 gtagaatggc caccgccaaa tggcgaattc ttattttttc tcgcgggtaa tacccttcca
    23821 ctacttaagt ctttttaacg tattcaggtg gagattcagt tatgcaaata gtcatgtttg
    23881 acaggcagtc aatatttatt catggaatga aaatcagttt acagcagcgt attccaggag
    23941 tgagtattca gggggtcagt caggcagacg agttatggca aaagctggaa agttaccctg
    24001 aagccttagt tatgctcgat ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa
    24061 aaaccgtggt gcagtttcct gaggttaagg tgttaatcac ggcgacggat tgcaataaac
    24121 ggtggttaca ggaagttatc cattttaatg tgctggccat tgtgcctcgc gattcaaccg
    24181 tcgagacatt tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt ctgcctggag
    24241 actggcggac gacgccggaa aaggacatta aagatctcaa atcgttaagc gcccgccagc
    24301 gggagatttt aaccatgtta gcagcgggcg aatcaaataa ggagatcggc agagcgctga
    24361 atatcagtac cggaacggta aaagcgcatc tggaatccct gtatcgccgt ctggaagtga
    24421 aaaatcgcac ccaggcggca atgatgttaa atatctcctc c
//
LOCUS       DADLZM010000052        24189 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLZM010000052 DADLZM010000000
VERSION     DADLZM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.052"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(22..594)
                     /locus_tag="JNY73_002018"
     CDS             complement(22..594)
                     /locus_tag="JNY73_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048039.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JNY73_002019"
                     /pseudo
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JNY73_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNY73_002020"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNY73_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY8048040.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNY73_002021"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNY73_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY8048041.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JNY73_002022"
     CDS             complement(3556..5337)
                     /locus_tag="JNY73_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY8048042.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JNY73_002023"
     CDS             complement(5330..7102)
                     /locus_tag="JNY73_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY8048043.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIHEEAVDA"
     gene            complement(7132..7590)
                     /locus_tag="JNY73_002024"
                     /pseudo
     CDS             complement(7132..7590)
                     /locus_tag="JNY73_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNY73_002025"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNY73_002025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY8048044.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLSSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="JNY73_002026"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="JNY73_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="JNY73_002027"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="JNY73_002027"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY8048045.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /locus_tag="JNY73_002028"
     CDS             complement(11173..11571)
                     /locus_tag="JNY73_002028"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY8048046.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="JNY73_002029"
     CDS             complement(11665..12036)
                     /locus_tag="JNY73_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048047.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNY73_002030"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNY73_002030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8048048.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNY73_002031"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNY73_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY8048049.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNY73_002032"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNY73_002032"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY8048050.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNY73_002033"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNY73_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY8048051.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNY73_002034"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNY73_002034"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY8048052.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNY73_002035"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNY73_002035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY8048053.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNY73_002036"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNY73_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY8048054.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="JNY73_002037"
     CDS             21807..22385
                     /locus_tag="JNY73_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8048055.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="JNY73_002038"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="JNY73_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY8048056.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5670 a         6659 c         5833 g         6027 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgctag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg aagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcactc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gtgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cagcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ctgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gcgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctaatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 cgaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcacgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgactgtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgttat
//
LOCUS       DADLZM010000053        23748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLZM010000053 DADLZM010000000
VERSION     DADLZM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.053"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="JNY73_002039"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="JNY73_002039"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY8048057.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="JNY73_002040"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="JNY73_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY8048058.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="JNY73_002041"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="JNY73_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY8048059.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="JNY73_002042"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="JNY73_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY8048060.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3880..3924
                     /locus_tag="JNY73_002043"
     CDS             3880..3924
                     /locus_tag="JNY73_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY8048061.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="JNY73_002044"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="JNY73_002044"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY8048062.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="JNY73_002045"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="JNY73_002045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY8048063.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARCHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEATEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREVDLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="JNY73_002046"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="JNY73_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY8048064.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="JNY73_002047"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="JNY73_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY8048065.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="JNY73_002048"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="JNY73_002048"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY8048066.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="JNY73_002049"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="JNY73_002049"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY8048067.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="JNY73_002050"
     CDS             10418..10882
                     /locus_tag="JNY73_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8048068.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="JNY73_002051"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="JNY73_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13331
                     /locus_tag="JNY73_002052"
     CDS             11895..13331
                     /locus_tag="JNY73_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY8048069.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMPMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNY73_002053"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNY73_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY8048070.1"
                     /translation="MRYTDSRKLTLETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNY73_002054"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNY73_002054"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY8048071.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNY73_002055"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNY73_002055"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY8048072.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="JNY73_002056"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="JNY73_002056"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY8048073.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNY73_002057"
                     /pseudo
     CDS             complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNY73_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="JNY73_002058"
     CDS             complement(20164..20457)
                     /locus_tag="JNY73_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY8048074.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="JNY73_002059"
     CDS             complement(20454..21743)
                     /locus_tag="JNY73_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8048075.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21769..22736)
                     /locus_tag="JNY73_002060"
                     /pseudo
     CDS             complement(21769..22736)
                     /locus_tag="JNY73_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22756..23520)
                     /locus_tag="JNY73_002061"
     CDS             complement(22756..23520)
                     /locus_tag="JNY73_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY8048076.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5856 a         5784 c         6261 g         5847 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgttg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagcgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagttgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtct aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgccgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaagcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaatcaatg cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt ggcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 ccccagcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagggg
    22321 ctgttgatct tttcctgagc gaacgctaac ccgccataca tccggtgttc ggcgcaaatg
    22381 tgaccatcga caatatccac cgccgtggca tcgttcacca gcgccgcatt cagttgcaca
    22441 cttaaccgtg ctgccagagc tttaccacgt ttcgtcgcgg ccaacagcaa catagcgggg
    22501 tgtttatctt tcagcagggc agcaatactt tcggcgtaat tttcagtgcg ttgcagcgcg
    22561 tcgttttgct caagaacata aatacatttt ggaccataag gcataactgc ctgcgcctgg
    22621 tcggtatttt gtacaatggc atacacctgt tggccccatt gctgagcgcc gccaaacagt
    22681 tcagcataac gttcaggatt atcgctaaag acccagacgc tgtttaattg actcatcgtt
    22741 tcttctccgt aggcttcagt tcagggcttt cttcagatgt tccgccagct cggcaatggc
    22801 ctccggcgaa tcgttatcga gaatgatgtg cttacgttct gtttgcggcg gtacgcgaat
    22861 gccgaccagt tcagcaagtg gcgcgctctg gctccagtca atatcacttg cctgccactg
    22921 atttaccggt tttttacccg cgccgagaat ggctttcatc gaaggaatgc gtggcacgtt
    22981 aatatcggag gtgacgcaga gcacggctgg aacagagagt tcaataactt caacatcatc
    23041 ttcaagcgtg cgttcaatca ccagtgtatt gccctgacgc tgaatagcac tcactgcatt
    23101 aatcaccgga agttgcagaa tttctccgac cagcaagcca acctgctggg cataaaggtc
    23161 gccggaacct tcaccaaaga tcagtaaatc gaagccgatc ttttcaattg ctgccgccag
    23221 cgcttttgcg gtatcgagag gcagtgcatg ttcaagttgc gcatcctgca ccaaatacag
    23281 gctgtgcggc ccgcgggata gcacgtcttt gcgcactttc gagttctgca acaatgagcc
    23341 gccaacggtc agcgcggcta tctcatcgtc atctgttgcg agctggcttg cagcttcaat
    23401 ggcattgaga tcgaactggc tgattttggc gtcggcattg tcgaaattca gggtgtattc
    23461 tggagtgaca acaatgtcct gttcttcagg caccagctta aagcaggtta ttattttcat
    23521 gatttctcct gtgagtcgca tacggaacca tctccgatgg aaggtaaaaa tggcctgaaa
    23581 atagtgtgcg atagcggtgc ggggggttcg acggagcaag ctgttttcct tgccaacaat
    23641 cttgcaaaaa aaatgcgatt gctaaaacaa aagtgcaatt tgcggcaata tagttattcg
    23701 gtaatgctgc caacttactg atttagtgta tgatggtgat tttaaggt
//
LOCUS       DADLZM010000054        22959 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLZM010000054 DADLZM010000000
VERSION     DADLZM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.054"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..461)
                     /locus_tag="JNY73_002062"
     CDS             complement(<1..461)
                     /locus_tag="JNY73_002062"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY8048077.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMV"
     gene            1066..1479
                     /gene="hns"
                     /locus_tag="JNY73_002063"
     CDS             1066..1479
                     /gene="hns"
                     /locus_tag="JNY73_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY8048078.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1623..2531)
                     /gene="galU"
                     /locus_tag="JNY73_002064"
     CDS             complement(1623..2531)
                     /gene="galU"
                     /locus_tag="JNY73_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY8048079.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2733..3746)
                     /gene="rssB"
                     /locus_tag="JNY73_002065"
     CDS             complement(2733..3746)
                     /gene="rssB"
                     /locus_tag="JNY73_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY8048080.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFHDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(3838..4743)
                     /gene="rssA"
                     /locus_tag="JNY73_002066"
     CDS             complement(3838..4743)
                     /gene="rssA"
                     /locus_tag="JNY73_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY8048081.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            4856..5314
                     /locus_tag="JNY73_002067"
     CDS             4856..5314
                     /locus_tag="JNY73_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY8048082.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLSVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5364..6206
                     /gene="purU"
                     /locus_tag="JNY73_002068"
     CDS             5364..6206
                     /gene="purU"
                     /locus_tag="JNY73_002068"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY8048083.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6366..6450
                     /locus_tag="JNY73_002069"
     tRNA            6366..6450
                     /locus_tag="JNY73_002069"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6400..6402,aa:Tyr,seq:gta)
     gene            6497..6628
                     /locus_tag="JNY73_002070"
     ncRNA           6497..6628
                     /ncRNA_class="other"
                     /locus_tag="JNY73_002070"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6812..7489)
                     /gene="narI"
                     /locus_tag="JNY73_002071"
     CDS             complement(6812..7489)
                     /gene="narI"
                     /locus_tag="JNY73_002071"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY8048084.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7489..8199)
                     /gene="narJ"
                     /locus_tag="JNY73_002072"
     CDS             complement(7489..8199)
                     /gene="narJ"
                     /locus_tag="JNY73_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY8048085.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8196..9734)
                     /gene="narH"
                     /locus_tag="JNY73_002073"
     CDS             complement(8196..9734)
                     /gene="narH"
                     /locus_tag="JNY73_002073"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY8048086.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9731..13474)
                     /locus_tag="JNY73_002074"
     CDS             complement(9731..13474)
                     /locus_tag="JNY73_002074"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY8048087.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSCSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(13867..15258)
                     /gene="narK"
                     /locus_tag="JNY73_002075"
     CDS             complement(13867..15258)
                     /gene="narK"
                     /locus_tag="JNY73_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY8048088.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15597..17393
                     /gene="narX"
                     /locus_tag="JNY73_002076"
     CDS             15597..17393
                     /gene="narX"
                     /locus_tag="JNY73_002076"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY8048089.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17386..18036
                     /gene="narL"
                     /locus_tag="JNY73_002077"
     CDS             17386..18036
                     /gene="narL"
                     /locus_tag="JNY73_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY8048090.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18037..19431)
                     /gene="ychO"
                     /locus_tag="JNY73_002078"
     CDS             complement(18037..19431)
                     /gene="ychO"
                     /locus_tag="JNY73_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY8048091.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19616..19969
                     /gene="ychN"
                     /locus_tag="JNY73_002079"
     CDS             19616..19969
                     /gene="ychN"
                     /locus_tag="JNY73_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY8048092.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20013..20708)
                     /locus_tag="JNY73_002080"
     CDS             complement(20013..20708)
                     /locus_tag="JNY73_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY8048093.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(20866..21096)
                     /gene="chaB"
                     /locus_tag="JNY73_002081"
     CDS             complement(20866..21096)
                     /gene="chaB"
                     /locus_tag="JNY73_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY8048094.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSTWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21366..22466
                     /gene="chaA"
                     /locus_tag="JNY73_002082"
     CDS             21366..22466
                     /gene="chaA"
                     /locus_tag="JNY73_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY8048095.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            22871..>22959
                     /locus_tag="JNY73_002083"
     CDS             22871..>22959
                     /locus_tag="JNY73_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY8048096.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVS"
BASE COUNT         5746 a         5999 c         5539 g         5675 t
ORIGIN      
        1 accatgcttg ctttacggcc acaaaaacag atggttttta attcaaccag tttgtcggac
       61 catgccagta agtattggct gccaataaat aattcacctc gaaaatcggt acgtaaacca
      121 taacaaagta cgggtatatc gagttgatcg acaacctccg ataattcata tacttgttgt
      181 ctggttaaaa actggcattc atcaaccagt acgcaatgaa ttgcctgctg ttcatgttcc
      241 gcacgaatct catcaaataa tgatgaattt tggttaaata attttgcagg cgatgacaaa
      301 cctatacgcg aactgacttt cccggcacca aagcgatcat caatttctgc cgtatatacg
      361 acagtgcgca tgccgcgttc ctggtaattg tatgaagatt gcaacaatgc tgtagactta
      421 cccgcattca ttgcggaata gtagaaatat agctgtgcca tcagccacag gccctcaatg
      481 atatgaaata aagttggctg gagtttatca taattcgtca gtttttcagc tcaacagtat
      541 gcacagaagt gggtatatgc gttctccctt acgaagcctt gcataatcct tctgagctat
      601 cattacaact gcctcgcttg ttataagcgg gtaaatgact gctggtaact attcacaatc
      661 tttaacctgt tgcgcaagta atagccctct gttgacctcc aggagatagt gcaatactaa
      721 gtccatgctc ttattgcgac tgttctactt ttcatcattc gcttaatagg gaattctcgt
      781 aaacacaact aatacagaag actgaaaggt cgtcagccta cgataatctc cccataaaat
      841 gtgacatgaa tcaggaagtt ttaacctcac gtgctgcgaa atcatcggtg taaatagggc
      901 tatatgccgc gtcttttctg gctaatttta tgaaaagata tttattggcg gcacaaaata
      961 aagaacaatt ttgaattcct tacattcctg gctattgcac aactgaattt aaggctctat
     1021 tattacctca acaaaccacc ccaatataag tttgagatta ctacaatgag cgaagcactt
     1081 aaaattctga acaacatccg tactcttcgt gcgcaggcaa gagaatgtac acttgaaacg
     1141 ctggaagaaa tgctggaaaa attagaagtt gttgttaacg aacgtcgcga agaagaaagc
     1201 gcggctgctg ctgaagttga agagcgcact cgtaagctgc agcaatatcg cgaaatgctg
     1261 atcgctgacg gtattgaccc gaacgaactg ctgaatagcc ttgctgccgt taaatctggc
     1321 accaaagcta aacgtgctca gcgtccggca aaatatagct acgttgacga aaacggcgaa
     1381 actaaaacct ggactggcca aggccgtact ccagctgtaa tcaaaaaagc aatggatgag
     1441 caaggtaaat ccctcgacga tttcctgatc aagcaataat cttttgtaga ttgcacttgc
     1501 ttaaaatccc gccagcggcg ggatttttta ttgtccggtt taagacaatt taataagaat
     1561 aagctataaa aaaacggcgt cgattgctca acgccgtttc gtggataaca ccgatacgga
     1621 tgttacttct taatgcccat ctcttcttca agccaggctt taaattccgt gccaagggtg
     1681 ttatgacgaa taccgtattc aacgaaggcc tgcatgtaac ctaatttatt accgcagtca
     1741 tggctcttcc ctttcatatg ataggcttcc accgtttctt tttcgatcag catatcaatt
     1801 gcgtcggtga gctgaatttc atcaccagct cccggagggg tttttgccag caacggccaa
     1861 atatccgcgc taagtacgta acgacccaca atagcgagat tagacggcgc aacatccgct
     1921 ttcggttttt ctaccacacc aaccatcggt acgctttcac ccggcgctaa ttcaacgcct
     1981 ttgcaatcca caacgccata tgcggtcaca tcagcaaccg gttcaaccat gatctggcta
     2041 tgacccgttt catcaaagcg gcggatcatc tctgccaggt tatcctgtga caaatcggat
     2101 tcatattcat ccagaataac atcaggcaaa ataacagcta ccggttcatc acccactacc
     2161 gggtgagcac acaataccgc gtgtcccagg cctttcgcca gaccctgacg aacttgcata
     2221 atagtcacgt gcggtggaca aatagactgc acttcatcaa gcagttgacg ttttacacgt
     2281 ttttccagca ttgcctccag ttcaaaactg gtatcaaagt ggttttcaat agagttttta
     2341 gatgagtgtg taaccagcac aatttcagta atgccagccg caatacattc attcacgacg
     2401 tattgaatta atggcttatc gacaagtggc agcatctctt tcgggatggc tttcgtcgcc
     2461 ggcaacatcc tggttcctaa tcccgcaacg gggataacgg cttttttgac tttcgtatta
     2521 atggcagcca tttaaattct cctggactgt tcgtgttttg aacgtgttca tatttctgta
     2581 tcgcatccca gtatatcagc accccgaagg ggctaagttc tgaaaaggag gcgagatagc
     2641 ataattaaga caattactta tacgaatggc gacgatagta ccaccagccg acattagcag
     2701 gtaatgcaaa tttagcccgc gttatcgttt gctcattctg cagacaacat caagcgcagt
     2761 cgaccaccgg ttccccatat ttggcattgc caggcatcgc atcgctggct caattgattc
     2821 aaataagcgt tacctaaagt gcctaacgga acaccattac tgatttgcac ctggtgttcg
     2881 ccggtattta acgtcgcatt cagacccgca gaaaccagaa tgagattttt cagttcgcga
     2941 tgataatagc caactaatag cggaaactgc cccggcagat tggcctgacg aagtaaatgg
     3001 tttacctgct tcaataacgc gcccaactct ggcaaccgtt gattttggtg tgcaagctgt
     3061 tcctgtaata atccgttaaa caatgcgcgt aataacaagg cagcaagtac gccattatgt
     3121 ccagctcggg tgacatcaag gcaataaaat gccagatcgt tttccgaaag tgcggcaata
     3181 tcaagcacca ggccgggttt gtccgcggca accaattgac ggtaattaac ccggcaatgg
     3241 gaaatcacct gctgaaccgg cggttgtagt tcctgtaata atttcgccgc tgcggcaggg
     3301 ttatcaacca ttgcatccca gtcgtgaaaa agcctttcct cttcctcaac gcgcgaatta
     3361 aacatgctgg gatagagaca ggcaaaaacc atctcgcgca agcgattcag atctttaact
     3421 ggtttcagca aaacatcttc aacgcccaga cgtaacgctt tggcaatatc tgccatattt
     3481 tcagtggcag atatcaccag aactggggtc tggtcgcctc tgttacgtat atgctccagc
     3541 agtttaagcc cgttcattcg tggcatcgcg atatcacata tcatcaggtc tggagtgaaa
     3601 cctcccagca actcaagggc atccacccca tcagccgcca gtaccgttgt cgctcccaat
     3661 gaggaaaacc atgaatccag aagcgagcga aatacctgct catcttcaac aatgagaatc
     3721 tgttttccga ccaatggctg cgtcatgttc tctcccctga ccggctttac tcaatagtgg
     3781 catgctattg ccacctgcgc ctgtcagaat taacttaagt gtagataaaa attctggtca
     3841 aatgttggtg cgtaccaacg gcaaaagttc gtccattttc ctttccactg ccagctgtcc
     3901 ggccgcaatg gcagcgtgcg cgcgatggaa atcaagcgta gatatttgcg ggcaaacagg
     3961 ttgaatcaga atatcgggcg gatcacctgc catgcggttc cttttaaggc ggttctccaa
     4021 cacctggata gacgtggtca taatctctgt tgccgttggc gctgtcacag cccgacgtgt
     4081 cgttatgctg cctaaccttt ctttcagacg cgcatgccac ggtagagaat caccattctc
     4141 gctattttct tcactgacat taaaggagag cagatcttgt tgcatcaaat gagcatcgtg
     4201 ctgcaggtcg accgctatca caatatcagc ccccaatgca cgcgtaagag aaataggaat
     4261 tgggttaact actgccccat caaccagcca gtagccgtta tgtgcaacgg gtgccatgag
     4321 tcctggaata ctgcatgatg cgcgaatagc aagatggaga tcgccttcag taaaccataa
     4381 ttcacgcccc gtacttaaat tggtggcaac agccgcaaag cgacgggaac aattttcgat
     4441 ctctgtttcc ggcattattt cgcgatattg attgaagaca cgctcgccgc gcagtaaccc
     4501 accgcgctgc caggagaggt ccatcaggcg taaaacgtcc caataactga aagaggttac
     4561 ccaatcttcc agcgcagata atcgatcgca tgcataggcc gcgcccacca gcgaaccaat
     4621 tgaacatcct gcaacgatat caatttcaat acccactttt tttagcgcat taataacgcc
     4681 aatatgcgac caacctctcg ccgcgccaga tcccagcgcc agccctattt ttatctttct
     4741 cattattccc gcaagattcc cctggaatgc aatcgtagcc acatcgctac tgctcggtta
     4801 acatagtgct actcaggcgg ttaatacgcc gttattgttt ccagggagat tattggtgtc
     4861 tcagctttgt ccctgtggta gtgctgtcga gtatagccta tgttgccacc cttatgtgtc
     4921 tggtgaaaag gttgcacctg atccagaaca tctcatgcgt tcgcgttact gcgcttttgt
     4981 gatgcaagac gctgattatt taataaagac ctggcatccc tcttgtggag cagcggcgtt
     5041 acgtgccgaa ttgatgaccg gatttgcgca tactgaatgg ttgggattga gcgtatttga
     5101 acattgttgg caggacgccg acaatatcgg atttgtcagt tttgttgctc gatttaccga
     5161 aggtggcaaa acaggtgcaa taattgaacg ttcgcgattt ttgaaagaaa acggtcaatg
     5221 gtactatatt gacggtacac gtccgcagtt tggtcgcaac gatccctgcc cttgtggttc
     5281 aggtaaaaaa tttaaaaagt gctgcggcca ataatggttg acggtacggt ttagcaaaca
     5341 ctctcaacaa ggtttttcca gcaatgcatt cactccaacg taaagttctg cgtactattt
     5401 gtccggacca aaaaggtctg atcgcacgta ttaccaatat ttgctacaag cacgagttaa
     5461 atatcgtaca gaacaatgaa tttgttgatc accgtaccgg gcgctttttt atgcgcacgg
     5521 aactggaagg gatttttaat gattccaccc tgctggcgga tctcgatagc gcattgccag
     5581 aaggctccgt gcgtgagctg aatcctgccg gtcgtcgccg gatagtgatt ctggtcacta
     5641 aagaagcgca ttgccttggc gatttgttga tgaaagccaa ttacggcggt ctggatgtcg
     5701 aaatcgcggc agttattggt aaccacgata ctttacgttc tctggttgag cgttttgata
     5761 ttccgtttga gctggtaagc catgaagggt taagccgcaa cgagcacgat caaaagatgg
     5821 cggacgccat tgatgcttat caacctgact atgtggtgct ggcgaagtat atgcgggtat
     5881 taacgccgga atttgtggca cgcttcccga ataaaatcat caatattcac cattcattcc
     5941 tgcctgcgtt tatcggcgca cgtccttatc accaggccta tgaacgtggt gtgaagatta
     6001 ttggcgcaac cgctcactat gtgaatgaca atctggacga aggcccaatc atcatgcagg
     6061 acgttattca tgtcgatcat acctacacag ctgaagatat gatgcgcgca ggtcgtgacg
     6121 tcgagaaaaa cgtcttaagt cgtgcgctat acaaagtact ggcgcaacgc gtctttgttt
     6181 acggtaatcg gacgattatt ctttaatcgc cagcaaaaat aactggttac ctttaatccg
     6241 ttacggatga aaattacgca accagttcat ttttctcaac gtaacacttt acagcggcgc
     6301 gtcatttgat atgatgcgcc ccgcttcccg ataagggagc tggctagtaa aaagcattac
     6361 cccgtggtgg ggttcccgag cggccaaagg gagcagactg taaatctgcc gtcatcgact
     6421 tcgaaggttc gaatccttcc cccaccacca tcactttcaa aagccccgga attctcaaac
     6481 gaatctgcaa tcaaatattc tgcccaagcg gggaaggatg agaagcttcg accaaggttc
     6541 gacttgagcg ccagcgagag agcgttgccg caggcaacga cccgaagggc gaagcgcgca
     6601 gcgctgagta atccttcccc caccaccatc gcttccttga ataatcaaaa cctgttactc
     6661 cgagctaatt ttgcccgatg gcaccacgtt taccgggcct acaatatcct cccctgtagg
     6721 tcggataagc gaagcgcatc cgacaactca ccccgcaccg tgcccataaa aaaaccccgc
     6781 cgaagcaggg tctatgtgaa ctaaaattcg tttagtgacg agcgcgcacc agctggtact
     6841 tacgtgtcag atactccacc ggtacgctcc agatgtgtac cagacgcgag aacgggaaca
     6901 gcaggaataa cgtcatcccc agcaccaggt gcagacggaa gataaacgcc acaccatcga
     6961 ggtgttgaga agcgccaccg tggaaggtca ccaccgactg cgcccagcca accagtttca
     7021 tcatctcgct accgtccata tgctgagcgg agaacggaat ggtcagcagg cccagcgcgc
     7081 actggataac gagcagcgac aggatcagga tatccgctcc ggtagtggtt gcacgcacgc
     7141 gtgggctgaa catacgacgt ttcagcaaaa gcacgccgcc aatcagacac agcacgccgc
     7201 tggcaccacc agcaaacatc gccattttct gtttcacttc aatcggcagc caggcttcat
     7261 acatccagtg cggcgtcaac ataccgaaga agtgaccgac aaaaatcccc agaataccga
     7321 tatggaacag gttcgacgcc aggttcatcc ctttgcgatc cagcatctgg ctggacgccg
     7381 cgcgccaggt gtactgcccg tagtcataac gcagccagct accaatcagg aagaccgccc
     7441 cggctatgta cgggtagata tcaaagaaga acatattcag gaattgcatt agtgctgtcc
     7501 tccggtggtg atattcagat attgcggcgc gacggcaccg gcaaagcgac gctgatgagc
     7561 agtgattgct gaatcaccgc agcctttgtc agcaaagaat ttaacctgct cttcttccca
     7621 gacagcatcc agcgcctgcg gcgtatcatc gcgcgcttcg tcggcaattt tttccgccac
     7681 tttgtcgctg tcgatagcgg tattggccaa tttcagcagc agatcaaaca tcacggcata
     7741 acggctttca cgctgttgca gacgcgcgct cagcaatgcc agaatcggcg cgatatcttt
     7801 caaaccttcc acggcttcgc tttgcggcag ctgcgacagg tactccagat acagcggcag
     7861 atggtccggt aattcgcggc tgtttaactg caagccgtgc tgctcgtact gcgccagcag
     7921 gtccaccatc gcctggccgc ggtcacggga ttcaccgtga acatgttcaa atagcaacag
     7981 tgacgtggcg cggccacggt cgaacagttc gctgtactgg acctgggcat cgagcggatc
     8041 catcgccgtt aaatcgcgca ggaaaatgcc cagcgcatgg gcatcttctt tcgacagatt
     8101 tttcgacgca gcaatcgcct caaacatctc ttgttgatgc tgccataagg cagcatccgg
     8161 atattcaagg agacgcgata caatcacgag ttcgatcatg gatgcggctc cgttttgctg
     8221 gtcacatcga tggcatcgat acgacggctg ttgaacagat tgaatttggt atctgagccg
     8281 tggcagccat caccaaaggt aaagccgcag ccatttttct ccgggaaggc ttcccgtgcc
     8341 agttcacgat gactactcgg caccacaaag cgatcttcgt agttagcaat cgccagataa
     8401 cggtacatct cctgtgcctg ggcttcggtc agaccgacct cttccagcgc acgggtatca
     8461 actttaccgt caacagtttc agcacgtttg taatgacgca tcgccagcat acgtttcagc
     8521 gcacgcagta ccggtttggt atcaccggcg gtcagcagat tcgccagata ctgtaccgga
     8581 atacgcaagc tttcgacgtc tggcagaatt ccgttgctgc ccagctcgcc cgcgtctgct
     8641 gcagactgaa tcggagacag aggcggcacg taccagacca tcggcagtgt gcgatattct
     8701 ggatgcagcg gcagcgccag tttccattcc atcgccattt tataaaccgg cgactgctgt
     8761 gcagcttcaa taacgctcag cggaataccg tctttaatcg cctgctcgat gactttcgga
     8821 tcgttcggat cgaggaacac gtccagctga cgctggtaga gatctttctc gttctcggtg
     8881 ctggctgcac gttcaatcgc gtcggcatcg tacagcagca cgccaagata acggatacga
     8941 ccgacacagg tttctgagca cacggtcggc tgacccgctt caatacgcgg atagcagaag
     9001 atgcacttct cagacttacc gctcttccag ttgaagtaga tttttttgta cggacatccg
     9061 gtgatgcaca tacgccagcc acggcattta tcctggtcga tcaggacgat gccatcttct
     9121 tcacgcttgt aaatcgcacc gctcgggcag gtcgccacac atgccgggtt caggcagtgt
     9181 tcgcacaggc gcggcaaata catcataaag gtgttttcga actggctata catcgccttc
     9241 tggatgttgt cgaagttctt gtctttcgcc agtttatcaa actcaccgcc caggtcatct
     9301 tcccagttcg gccctttttc gattttcgcc atccgttccc cggtaatcag cgaacgcgga
     9361 cgggcaatcg gctgcgattt gctgccttcc ggcgcggtat gcaggttctg ataatcaaaa
     9421 tcgaacggct cgtaataatc gtcgatcccc ggcagatgcg ggttagcaaa gattttaccc
     9481 agcagcatgg ctcggttacc catgcgcggt tgcagtttgc cattgatttt acggatccag
     9541 ccgcctttgt atttttcctg gttttcccag tcagtcggga agccctggcc cggcttggtt
     9601 tccacgttgt tgaaccacgc gtattccacg ccttcacggc tggtccagac gtttttacag
     9661 gtaactgaac aggtgtggca gccgatgcac ttatcgagat tcaacaccat gccgacttgt
     9721 gaacgaattt tcattttacg ctctcctgta cctggtcatt gccttcgcca tctaaccagt
     9781 caatgttctt catcttacgc actacaacaa actcatcgcg gttagaacct acggtgccgt
     9841 agtagttaaa gccgtatgcc agatgggcat agccgccgat catatgcgtc ggtttcggcg
     9901 tgatacgggt gaccgagtta tggataccac cacgctgttg ggtaatttcc gaaccaggca
     9961 ggttaacgat acgttcctgc gcgtggtaca tcatggtcat ccctgccgga acacgctggc
    10021 tgacaaccgc acgggcagtc agtgcaccgt tgctgttgaa gacttcaatc cagtcgttat
    10081 cggcgatacc cagatctttg gcatcggctt cactcaacca gaccaccgga ccaccgcgac
    10141 ctaaagtcag catcagcagg ttgtcgctgt aggtggagtg gataccccac ttctggtgcg
    10201 gcgtcaggaa gttgagcgct ttttccgggt tgccgttgga tttctggcct atcacttctt
    10261 tcaccgaacg ggtgtcgatc ggcggacgat aaaccagcag gctttcaccg aaatcacgca
    10321 tccactggtg atcctgatac agttgctgac gaccagagag cgtacgccat gggatcagct
    10381 cgtgaacgtt ggtgtaaccg gcgttgtaag aaacgtgttc atcttccaga ccagaccagg
    10441 tcgggctgga gataattttg cgcggctgtg cctgaatatc gcggaagcgg atcttctcgt
    10501 cttctttatt cagcgccaga tgcgtatggt cacgaccggt aaattcgctc agggcagccc
    10561 aggctttcac ggctacctga ccgttggttt ccggtgccag cgtcaggatc atctctgccg
    10621 catcaattgc ggtgttcagc atcggctggc cttttgccgg accttccgct ttggtgtagt
    10681 tgagcttacg cagcagatcc atctcgctct gggtgttcca ggcaatccct ttaccgccgt
    10741 taccgatttt ctccatcagc gggccgatag aggtaaagcg ttcgtaagtt gccggataat
    10801 cgcgctctac gaccataatg tgcggtgcag ttttgcctgg gatcaggtcg cattcgcctt
    10861 ttttccagtc tttcacatcc agcggctgcg ccagttcagc ggcagagtcg tgctggatag
    10921 gcagcgtgac gatgtcggtt tctttaccca gatggccgac gcacacttcg gagaatttct
    10981 tcgcgatggc tttgtagatt tcccagtcgc ttttagcttc ccaggccgga tcgaccgccg
    11041 cagacagcgg gtgaataaac ggatgcatat ccgaagtatt catgtcgtct ttttcgtacc
    11101 aggtcgccgt cggcaaaatg atgtcggaat agagacaggt gctcgacaga cggaagtcca
    11161 gcgtaaccac cagatccagc ttgccttcca gaccattgtc ctgccagtcc acttcttccg
    11221 gcttcacgcc gccctgttgc cccagatctt taccctggat accgtgctcc gtccccagca
    11281 ggtacttgag cataaactca tgacctttac cggaagaacc gagcaggtta gaacgccaga
    11341 tgaacaggtt gcgcgggtgg tttttaccgt tttctggttg ttctgccgca aaacggatgg
    11401 aaccctcttt cagggatttc accgtatagt ccaccggatt catcccggct ttttccgctt
    11461 cgccagcgat agtcagcggg ttagtgccta actgcggtgc agacggcagc cagcccatgc
    11521 gttccgcacg gacgttaaag tcgatcaagt gtccggtata gcgggatttg tccgccatcg
    11581 gtgacagcaa ctcttccgcc gtgacggttt cataacgcca ctggctggag tggttataga
    11641 aataagaagt gctgttcatg tgacgcgccg gacgctgcca gtcaagggca aacgccagcg
    11701 gctgccagcc ggtttgcgga cgcagttttt cctgacctac atagtgcgcc cagccgcccc
    11761 cgctctgacc gacacagccg cagaaaatca gcatgttgat cagaccacga tagttcatat
    11821 cgaggtgata ccagtggttc agccccgcac cgacgataat catcgaacga ccgtgcgttt
    11881 tatcagcgtt atcggcaaat tcacgggcga tgcgaataat ctggctgcaa gaaacgccgg
    11941 taatctgctc ggcccaggcc ggggtataag ctttcacatc gtcatagctg gttgcacagt
    12001 taacgtcgtt caggccacgt tccagaccgt agtttgccag cgtcagatca taaacggtgg
    12061 tcaccagggc ggtgctgcca tcagccagtt gcaggcgttt caccggcagt ttgtgcagca
    12121 gcacgttttc cagttccact ttgttgaagt gttccgtgcc gtcgccacca aagtacggga
    12181 agcctacctc agcgatctca tcctggctac ccagcaggct cagttgcagc tcggtttctt
    12241 cgccagtttt gccgtcgcgc tgctcaagat tccatttgcc cttctcgccc cagcggaagc
    12301 caatagaacc gttcggcgca accatttcgc cattggtatt aaaggcgaca gttttccatt
    12361 ccggattgtt ttcctggccc agcgcatcaa ccagatcagc agcgcgcagc atacgacctg
    12421 cagcgtagta accgtcgcgt tcttccagca tcaccagcat cggcatgtcg gtgtagcgac
    12481 gcacatagtc ggtgaaatac tggcttgggt tgtcgaggtg gaattcacgc agcattacgt
    12541 ggcccatcgc cagcgccatt gccgcatcgg tgccctgttt cggtgccagc cacagatcgc
    12601 acagtttggc gatttcagcg tagtctggtg tgacggcaac agttttggtg cctttgtaac
    12661 gcacttcagt aaagaagtga gcatccgggg tacgcgtctg cggcacgttt gaaccccagg
    12721 cgatgatgta gctggagtta taccagtcag cagattccgg tacgtcagtt tgctcgcccc
    12781 aggtttgcgg agacgcagga ggcaggtcgc agtaccagtc gtagaagctt aagcaagtac
    12841 cgccaatcag cgagagatag cgtgcacccg atgcgtaaga aaccatcgac attgccggaa
    12901 ttggcgagaa accagcaaca cggtccgggc cgtagttttt gatggtgtaa acgttagatg
    12961 cggcgatcag ttcgttcacc tcctgccaag aagaacgaac aaatccaccg cgtccacgcg
    13021 cctgcttaaa gcttttcgct ttatcggcgt cttcaatgat agaagcccat gcctcaaccg
    13081 gatcgctatg cagcgccttc gcttcacgcc acattttcat caggcgtttg cgcatcatcg
    13141 ggtatttcag gcggttggca ctgtaaagat accaggagta gctggcaccg cgcgggcagc
    13201 cgcgaggttc atggtttggc agatccggac gggtacgcgg atagtcagtc tgctgggttt
    13261 cccaggtgac cagaccgttt ttgacgtaga ttttccagct gcaggagccg gtgcagttta
    13321 ccccgtgggt agagcggacg attttgtcat gctgccaacg ctggcgatat ccatcctccc
    13381 agtcacggtt ggtattgaga agctggccat gcccatcggc aaaggtttca cccttctgtt
    13441 tgaagtagcg aaaccggtcc aggaatttac tcatcggttt tctcctgtgg gagcctgtcg
    13501 gccctctgat aaatcgacat tgctaatgtt taagggcaag gtaacgctct gaaacagcgt
    13561 gggaattgat aacgatcaag agtgatgggg atgaaaaata aagtaaattc ctaagatacc
    13621 cctttaggat aattaacagt tacagcataa catgctgtaa attaagacgt tttatacctg
    13681 tagttattta aactattaag atttttatgc aaatgggtat taaggagtat tccccatggg
    13741 taacatatta atggctctta atgaaatccg gcgaaataag ccatataaca ctaatatgtt
    13801 gtaactaaat gacgcataaa aaaagcgcgg catcatgccg cgcaaaggat aatcaaatat
    13861 taagatttat tttttagaat gccgaccata taccgcccaa gtaatcacta cgcaggcgat
    13921 atagaaaatc aaaaatactt tcattgcacc tactggcgaa cccgttaatg ccagcgagct
    13981 accaaacgct ttcgggataa agaagccacc aatcgcgcca atcgcagaga tgaaacccag
    14041 cgccgctgcc gtgccggttg ccgcttcacg catcgcacgt tcgtcagaac ccccttctgc
    14101 tttcacgcga tccattgtca gtttacggaa gatcactgaa atcatctgga aagtggaacc
    14161 actacccagc ccagctgtca ggaacagcgc caggaagacc gcgaagaacg ccatgaagct
    14221 tccgccctgc ccgtcagtcg gtaaggtcag gaacagcagg ccgctgaaaa tcgccatcag
    14281 aataaagttc accagcgtga cacgagttcc gcccagacgg tcagataatg caccacctgc
    14341 agaacgcgcc agcgcaccaa taaacggccc gaagaaagcg tattgcagaa tctgaacatc
    14401 cgggaactgc gtttttgaca gcatagcaaa gcccgcggag aagccgatga aggagccgaa
    14461 ggttgccaga tacagcaggc tcataatcca cagatgaccc cgtttgagta ccggcaactg
    14521 ctccttgatg gaggctttcg aggtagcaag atcgttcatg ccaaaccacg ccgcaatggt
    14581 gaagatggca aggaacggca cccatatcca ggacgcattc gccagataca gctcagtccc
    14641 atccggctgt ttgacgccct ggctaccaaa tactgcgaaa atcgacagtg ataccaccag
    14701 cggagcaacc aactgcatga cgctgacgcc catgtttccc agaccaccat tcagacccag
    14761 cgcgccaccc tgcttctgtt tcggaaagaa gaagctgatg tttgccatac tggatgcgaa
    14821 gttcgcgcca gcaaaaccgc atagcagaga gatgatgatg aagacgctat aaggcgtgga
    14881 ggtatcctgc acggcaaaac ccagccagac gcaaggaata atcagaatac cggtgctgaa
    14941 cgccgtccag cgacgaccac cgaagatagg aaccataaag gagtatggaa cacgtaataa
    15001 cgcgccagaa accgaaggca gcgcagtcaa cataaatagc tgatcggtcg taaaattaaa
    15061 accgactttc ggtaagttca ccgcaacagc gctgaacaac atccatacgc aaaacgccag
    15121 cagcagacag ggaacggaaa tccacaggtt gcggctggca atacgttgac cgcgttgttg
    15181 ccagaacgca ggatcttccg gtcgccaatc tgttatgaca gctccagtag ccctttcgga
    15241 ggcagatgag tgactcatag acacctctga tactcgtttc gatgtctgcc accttagtgt
    15301 ctggagctaa aggcaatttg atgtaaatca aacgataaga taactttatc tatcattgat
    15361 atttatcatt acccatagtg agtacagtga tttcataaaa attatgagat ttttcacggt
    15421 gctgtagaat ccctaccctt accgatgtaa agcgactaac cacacggcaa ataaggagta
    15481 actctttccg gctatgggta tacttcagcc aatagccgag aatactgcca ttccagaata
    15541 tatcgtcaca ttcattaagg ttattgctca tttaaagcct gaaggaaggg gtttacatgc
    15601 ttaaacgttg tctctctccg ctcaccctgg ttaatcaggt tgcgcttatt gtgttgcttt
    15661 ctactgctat tggactggca gggatggcgg tttctggctg gctggtgcaa ggcgttcagg
    15721 gcagcgccca tgcgatcaac aaagcgggat cgctgcgcat gcaaagttac cgtctgttgg
    15781 cggcagtgcc attaagcgag aaagacaagc ccttaattaa agagatggaa caaacggcat
    15841 ttagcgccga gttgactcga gcagcagaac gagacggaca actggcgcaa ttacagggtt
    15901 tacaagatta ctggcgtaat gaactgatcc ctgcgctgat gcgtgcacaa aaccgagaaa
    15961 cggtgtcagc ggatgtcagc cagtttgttg ccgggcttga tcagctggta tctggttttg
    16021 accgcaccac ggaaatgcgt attgagacag tggtactggt ccatcgggta atggcggtat
    16081 ttatggcact tttactggtg ttcactatta tctggttgcg ggcgcgactg ctacaaccgt
    16141 ggcggcaact gctggcaatg gcgagtgccg tcagccatcg cgattttacc caacgcgcaa
    16201 acatcagcgg gcgcaacgaa atggcgatgc ttggaactgc gttgaacaat atgtctgcag
    16261 aactggccga aagttatgcc gtacttgagc agcgggttca ggagaaaacc gccgggctgg
    16321 agcataaaaa tcagatcctc tcttttttat ggcaggctaa ccgccgtttg cattcccgcg
    16381 ccccgctgtg tgaacgcctg tcaccggtac tcaacggctt acagaattta accctgctac
    16441 gtgatatcga actgcgggtg tatgacactg atgatgaaga gaatcatcag gagtttacct
    16501 gccagccaga tatgacttgt gatgataaag gctgccagct ctgcccgcgc ggcgtattac
    16561 ccgttggtga tcgcggcacg accctgaagt ggcggctggc tgactctcat acgcagtacg
    16621 gtattttgct ggcgaccctg ccgcaggggc gtcatcttag ccatgatcaa caacaactgg
    16681 tggataccct ggttgaacaa cttaccgcca cgctggcgct ggatcgccat caggaacgtc
    16741 agcaacagtt gatcgtgatg gaagagcgtg ccaccattgc gcgcgaactg catgattcta
    16801 ttgcccaatc tctctcttgc atgaagatgc aggtgagttg tttacagatg cagggcgatg
    16861 cgctgccaga aagcagccgc gaactgttaa gtcagatccg taacgaactg aatgcatcct
    16921 gggcgcagtt gcgtgaattg ctcaccacat tccgcttgca gctcaccgag cctggattac
    16981 gtccggcgct ggaagcgagt tgcgaagagt acagcgccaa atttggcttc ccggtgaagc
    17041 tggattatca attgccgcct cgtctggtgc cttcacatca ggcaatccac ttgttgcaaa
    17101 ttgcccgtga ggcattaagt aacgccctca aacattcgca agcgagtgag gtcgtggtga
    17161 cggtggcgca aaacgataat caggtcaaac tgaccgtcca ggataacggc tgcggcgtgc
    17221 ctgaaaatgc catccgcagc aatcactacg gcatgataat aatgcgcgac cgtgcgcaaa
    17281 gtttacgagg cgattgccgc gtccgccgtc gtgaatcagg tggcaccgaa gtggtggtca
    17341 cctttattcc cgaaaaaact ttcacagacg tccaaggaga tacccatgag taatcaggaa
    17401 ccggctacta tcctgctgat tgacgatcac ccgatgctgc gaactggcgt aaaacagctt
    17461 atcagtatgg caccagatat caccgtggtt ggcgaagcga gtaatggcga acagggtatt
    17521 gaactggcgg aatctcttga tcccgatctg atcctgttag atctcaatat gcccggcatg
    17581 aacggtctgg aaacgctgga taaactgcgc gaaaagtccc tctcagggcg cattgtggta
    17641 ttcagcgtct ctaaccatga agaagatgtg gtcaccgcac tgaaacgcgg cgcggatggc
    17701 tatctgttaa aagatatgga accggaagat ctgctgaaag cattgcatca ggcagctgct
    17761 ggcgaaatgg tattaagcga agcattaacg cctgttctgg ccgccagctt gcgcgctaac
    17821 cgtgccacta ctgagcgcga tgttaaccag ttaaccccac gcgagcgcga tattctcaag
    17881 ctgattgccc agggtttgcc gaacaagatg attgcccgcc gcctggatat caccgaaagc
    17941 acagtaaaag tgcacgtcaa gcacatgctg aagaaaatga agctcaagtc tcgcgtggaa
    18001 gcagcggtat gggtgcatca ggagcgcatt ttctgattac ggttcccagc gcagttcgtc
    18061 gtttgacaat gcatcgaacg gttcgacaag cgttagcgtg atctcattgg aggagacacg
    18121 ctgcccctgg ttatcttcca ccaccacaga caatcgccag tgattgcttg ccccttcccc
    18181 gttctgccag tcaggcatga tcagcgtcca gccctccgcg ctgttggctt gtgcgcccgg
    18241 cgtcaaactt aatatctgcg tatcgccctg ccaaatcagt tgccgaatac cgtaacgact
    18301 gcgcatttgt aatttcagcg gcactgtttc gccaggtttt agatcccacg gcggtgtcgc
    18361 cagaaacacc gttaacgttt ttcgctgtcg gtactcaaga gtcggtagat tatttcgctg
    18421 cggattgtca tagcgactac cacgtaacga ctgactttcg gcaacctcgc ccgcagaaag
    18481 ttgttttttg agcggtacac caaagcggta attaagattc agcccgaggt tattttgatt
    18541 ttcgccactt tccccctgtt tatgctgggc cgtcacagtg actaatggca caggggtgta
    18601 atttaatccc agactcaacg cgacgggatt gtgataaccc gtaccaaagt taaacaaatc
    18661 aacacgatca ccaaaatact gttctaggct gacactggta ttgaggtgtt gatagaacgg
    18721 catgcgcatc cgagctgtca ggtcgtaccc gcgcgccatc cgttgttcct gcgtggctgt
    18781 ctgttcatgc catgcagcaa acggctgata aaagtttgcc gataatcgca aatattcgcc
    18841 ccacgcttcg gcaccaaagc ccgctcgctg aagattttcg tcctgcaagt tgtcataaaa
    18901 agtgttataa cccaccagcc agttgccgcg cgcccagcgt tgcccaacgc ccacattgct
    18961 caccaaccca ttatcctgct gagtaagacc aagctggctc caggtgagat aacgatcatt
    19021 atcttgtaac ggcacaaacc agcttccacg actgccggtg aaatgtcctt cgttatccac
    19081 tttgacgtca acactggcat ttccccacgg tgatagccag gactctacgt gctgattaac
    19141 ctgttgacta agcgcgtcgc ggacttttcc caatgcgaaa gcttttgcct gctcgccagt
    19201 atccagcccg ttatcattca tactggtttc gccgaaatct ttcacaattt cagcaaagtg
    19261 tttttcccca tcatgatttt cgggagccat gcctaaatcc ggcagaccat cattgttatt
    19321 atcaaaggga tttgccgctt tttgctcgaa ggtagattgt gcgttagctg taccgcctgc
    19381 tacaagcaag agtaaataaa acgggataat gcgaggaacg aaacggctca aaacgtcggt
    19441 aacttccggt aataatgaaa aaccatagcc ttaaagaata acgattactg gcaacttttg
    19501 tcgtattttc tcgcgctttc aggaaaatcc ttaaaaatgt gaccgattgg tcagacgctg
    19561 cagaagcagt aagatagaag atgagtgtct aattccctga atacaggagt aaataatgca
    19621 aaaaatcgtg atcgttgcca atggcgcacc ttacgggagc gaatccttgt ttaacagctt
    19681 gcggctggcc attgcgttac gagagcagga gagcaatctg gatctgcgtc tgttcctgat
    19741 gtctgatgcg gtcacagccg ggttgcgcgg gcaaaaacca ggggaaggct acaacattca
    19801 gcaaatgctg gagatcctta ccgctcagaa tgtaccggtg aaattgtgca aaacctgtac
    19861 cgacgggcgc gggattagca cacttcctct gattgatggg gtggaaatcg gtactcttgt
    19921 ggaactggcg caatggacgc tgtcagccga taaagtgctc acattttaat gtttaagggg
    19981 gcgtctgacg ccccactatg agggaagttt actcaggcga atcccggcct taacacacca
    20041 tccggataat tctccgccag tagttttttt accgatacca gcaaatcatt cagcccatcg
    20101 tcctgcattc ccagtttgat aagctcctgt tccagtgaaa acaggtattg tgcattggtt
    20161 cccagcggac cgctcgccgc cgcaatcaac ggcgcgatga cctgagcgcg agtgtcagat
    20221 tcatattctg gatgtcgcgg gtccataata aacacaatgg cgtttactgt gcgtccatca
    20281 tcaagatcaa gctgacacca ggttggcaga taacagccgg taatcatctc gcgcttccac
    20341 aacagggtta gttcctgctc cagcgtctct tctggtagtc gataggcgac gcctgtggtg
    20401 cgtccgccct ctttcagtgc aagcatccgt cccggctggt gcgcagtccc gcgcccggcg
    20461 gtcaggcgca ggcaaaacgc gcgatgccat ccaaccagtg taccggtgca tgattcggta
    20521 aactccagtg ccggattcca catcaacgat ccataaccga agatccacac cggtccctca
    20581 tcaggtcgac aagccagcgt cgccgccagc gaagccgccc gttgttctgc tgaccataag
    20641 agtgattctt caatggcacc aaatgccgtt ttacaatcgg cattcatcaa gaaatcacgc
    20701 gttatcacct tacacctccg ccactgcata cttccctgcg acaacttttt ggtgtcttcc
    20761 agacattttt accgctcatt ttgaaaccac taacgggaca atatgcaatc cacggaccac
    20821 ttgcaaggca acaccgcacg aatagcttta actaagctac cggttttacg attttttatg
    20881 ccatttatca tcatcccctt tggcatattc atgcttcaca gcagcccagg ccactttatg
    20941 cgcggtttct tcgcgactgg cgtcatcacg ccgatcttct ttatctttat attgatccca
    21001 tgtgctgttg aacgcttctt tatagatatc ctgggcatga gacggtagaa cgtgctttac
    21061 gctttccggc agatcgcttt tcgttttata cggcatcgtt gactcctttt acaaagttga
    21121 acattaagta tggtcaacaa tatctactct tgccaaaaca taagaatttt catgtcagac
    21181 tgttcttaag aaacgctaaa acaaacattg tctaaacgta atacttattt ttatgcccca
    21241 atgagtaaaa aatcgacagc ggggtaaaaa ttcgtaaaat gttaccgata tccagacact
    21301 ttctgttatt tttcctacct attttttaca tttccgataa attcatctgc aaagggataa
    21361 ccataatgtc aaatgctcaa gaggcggtaa aaacccgcca caaggagact tcgcttattt
    21421 tcccggttct ggcgctggta gtgctgttcc tgtggggaag tagccagaca ctaccagtgg
    21481 tcattgccat caatcttctt gcgcttattg gttttttaag tagcgccttt agtgttgtcc
    21541 gtcatgcgga cgtattagcc catcgcctgg gagaacctta cggttcgctt attcttagcc
    21601 tttcagtggt tattcttgaa gtcagtttga tttcagcttt aatggcaacc ggcgacgccg
    21661 cgccaacgct aatgcgtgat acgctctatt caatcattat gattgttacc ggtgggctgg
    21721 ttggcttttc attattgttg ggcggtcgta agtttgccac ccaatatatg aatctgtttg
    21781 gtatcaagca gtatttaatt gcgctgttcc ccctggcgat aatcgtactg gtatttccaa
    21841 tggctctgcc tgcggcgaat ttttcaaccg gtcaggcgtt actggtagca ttaatttctg
    21901 cggcaatgta tggcgtattt ttgctgatcc aggccaaaac gcatcaaagt ttgtttgtct
    21961 acgagcacga agatgacagt gatgacgacg acccgcacca cggtaaaccg tctgcccata
    22021 gcagcctgtg gcatgctatc tggttgatta tccatctgat tgccgttatt gcagtcacca
    22081 aaatgaacgc cagctcgctg gagacattgc tcgacagcat gaatgcccct gttgccttta
    22141 ctggcttcct ggtggcactg ttgattctgt cgccggaagg tttaggtgcg ttaaaagcag
    22201 tgttgaacaa ccaggttcag cgcgcgatga atctgttctt tggttcagtg ttagcaacaa
    22261 tttcgcttac cgtacctgtc gtcacgctaa ttgcctttat gacgggtaac gaattgcagt
    22321 ttgcacttgg tgcgccagaa atggtggtga tggtggcctc tttagtgctg tgccatatct
    22381 ccttctccac cggacgtact aacgtactca atggcgcagc gcatctggca ctgtttgccg
    22441 cctatttgat gacgatattt gcctgacagt tatatctcct ccgatgacgg aaatcgggag
    22501 agataagata aattcctgct tattgtattt cttcatacta cctggacagc tcgttaatca
    22561 gaattgcgag cctgtaccac aacctgcacg aaaccactgc aacagctgtt gaaaagccgg
    22621 aatgtactct ttattgcaaa catttcaggg atgaagtgaa gatgaattga gacgtagtat
    22681 ccacaccaag ccggaattat ccggtgaggc gcaatgttgc gggggcttta tccctggtgg
    22741 cattggttgc tggaaagaga aaacccccgc acgttgagag gtaaaacctg acaacaaaac
    22801 gggggctaat cttgaaacca gacacttcaa gaatagccgc aaaatgttgt cattacaaca
    22861 aggcggctat atgacgctcg cgcagtttgc catgactttc tggcacgacc tggcagcacc
    22921 gatcctggcg gggattatta ccgcagcgat tgtcagctg
//
LOCUS       DADLZM010000055        22319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLZM010000055 DADLZM010000000
VERSION     DADLZM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.055"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="JNY73_002084"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="JNY73_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY8048097.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="JNY73_002085"
     CDS             831..971
                     /locus_tag="JNY73_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048098.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="JNY73_002086"
     CDS             985..1857
                     /locus_tag="JNY73_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY8048099.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNY73_002087"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNY73_002087"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY8048100.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNY73_002088"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNY73_002088"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY8048101.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNY73_002089"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNY73_002089"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY8048102.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNY73_002090"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNY73_002090"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAY8048103.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNY73_002091"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNY73_002091"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY8048104.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="JNY73_002092"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="JNY73_002092"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY8048105.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVITLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNY73_002093"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNY73_002093"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY8048106.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="JNY73_002094"
     CDS             complement(14044..15384)
                     /locus_tag="JNY73_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY8048107.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /locus_tag="JNY73_002095"
     CDS             complement(15386..16738)
                     /locus_tag="JNY73_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048108.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="JNY73_002096"
     CDS             complement(17172..17621)
                     /locus_tag="JNY73_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY8048109.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNY73_002097"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNY73_002097"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY8048110.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="JNY73_002098"
     CDS             complement(18421..18750)
                     /locus_tag="JNY73_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY8048111.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNY73_002099"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNY73_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY8048112.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="JNY73_002100"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="JNY73_002100"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY8048113.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="JNY73_002101"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="JNY73_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY8048114.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5511 a         5916 c         5294 g         5598 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccaag
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac ggtttttcca cccggcccca gaaccacggt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtca ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgattacc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gagcagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttag attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DADLZM010000056        22068 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLZM010000056 DADLZM010000000
VERSION     DADLZM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.056"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1980)
                     /locus_tag="JNY73_002102"
     CDS             complement(<1..1980)
                     /locus_tag="JNY73_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY8048115.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLHGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQEATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEEN"
     gene            complement(2073..2720)
                     /gene="rnt"
                     /locus_tag="JNY73_002103"
     CDS             complement(2073..2720)
                     /gene="rnt"
                     /locus_tag="JNY73_002103"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY8048116.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(2823..3230)
                     /gene="gloA"
                     /locus_tag="JNY73_002104"
     CDS             complement(2823..3230)
                     /gene="gloA"
                     /locus_tag="JNY73_002104"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY8048117.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(3311..4408)
                     /gene="nemA"
                     /locus_tag="JNY73_002105"
     CDS             complement(3311..4408)
                     /gene="nemA"
                     /locus_tag="JNY73_002105"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY8048118.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(4445..5044)
                     /locus_tag="JNY73_002106"
     CDS             complement(4445..5044)
                     /locus_tag="JNY73_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8048119.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLIFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5147..5386
                     /locus_tag="JNY73_002107"
     CDS             5147..5386
                     /locus_tag="JNY73_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY8048120.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5435..6331
                     /locus_tag="JNY73_002108"
     CDS             5435..6331
                     /locus_tag="JNY73_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY8048121.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGMLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            6412..6933
                     /gene="sodC"
                     /locus_tag="JNY73_002109"
     CDS             6412..6933
                     /gene="sodC"
                     /locus_tag="JNY73_002109"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY8048122.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6934..8916)
                     /locus_tag="JNY73_002110"
                     /pseudo
     CDS             complement(6934..8916)
                     /locus_tag="JNY73_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(8813..9700)
                     /locus_tag="JNY73_002111"
     CDS             complement(8813..9700)
                     /locus_tag="JNY73_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8048123.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIHAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALVQGHAGAMALCV
                     TQYHCHVSGADGCLLFESG"
     gene            complement(9703..9939)
                     /locus_tag="JNY73_002112"
     CDS             complement(9703..9939)
                     /locus_tag="JNY73_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY8048124.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            10140..10574
                     /gene="slyA"
                     /locus_tag="JNY73_002113"
     CDS             10140..10574
                     /gene="slyA"
                     /locus_tag="JNY73_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY8048125.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(10621..11088)
                     /gene="slyB"
                     /locus_tag="JNY73_002114"
     CDS             complement(10621..11088)
                     /gene="slyB"
                     /locus_tag="JNY73_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY8048126.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            11362..12471
                     /gene="anmK"
                     /locus_tag="JNY73_002115"
     CDS             11362..12471
                     /gene="anmK"
                     /locus_tag="JNY73_002115"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY8048127.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            12569..12898
                     /gene="mliC"
                     /locus_tag="JNY73_002116"
     CDS             12569..12898
                     /gene="mliC"
                     /locus_tag="JNY73_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY8048128.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGFSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            12957..13613
                     /gene="pdxH"
                     /locus_tag="JNY73_002117"
     CDS             12957..13613
                     /gene="pdxH"
                     /locus_tag="JNY73_002117"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY8048129.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            13742..15016
                     /gene="tyrS"
                     /locus_tag="JNY73_002118"
     CDS             13742..15016
                     /gene="tyrS"
                     /locus_tag="JNY73_002118"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY8048130.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            15078..15944
                     /gene="pdxY"
                     /locus_tag="JNY73_002119"
     CDS             15078..15944
                     /gene="pdxY"
                     /locus_tag="JNY73_002119"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HAY8048131.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHVLGIVRQVK
                     AANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVN
                     NVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMR
                     QPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRI
                     AKPEHYFSATKL"
     gene            complement(15988..16593)
                     /gene="gstA"
                     /locus_tag="JNY73_002120"
     CDS             complement(15988..16593)
                     /gene="gstA"
                     /locus_tag="JNY73_002120"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY8048132.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(16699..18201)
                     /gene="dtpA"
                     /locus_tag="JNY73_002121"
     CDS             complement(16699..18201)
                     /gene="dtpA"
                     /locus_tag="JNY73_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY8048133.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(18812..19447)
                     /gene="nth"
                     /locus_tag="JNY73_002122"
     CDS             complement(18812..19447)
                     /gene="nth"
                     /locus_tag="JNY73_002122"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY8048134.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKERVDI"
     gene            complement(19447..20142)
                     /locus_tag="JNY73_002123"
     CDS             complement(19447..20142)
                     /locus_tag="JNY73_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY8048135.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(20146..20766)
                     /gene="rsxG"
                     /locus_tag="JNY73_002124"
     CDS             complement(20146..20766)
                     /gene="rsxG"
                     /locus_tag="JNY73_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY8048136.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(20770..21828)
                     /gene="rsxD"
                     /locus_tag="JNY73_002125"
     CDS             complement(20770..21828)
                     /gene="rsxD"
                     /locus_tag="JNY73_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY8048137.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(21829..>22068)
                     /locus_tag="JNY73_002126"
     CDS             complement(21829..>22068)
                     /locus_tag="JNY73_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY8048138.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQAN
                     AEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         5523 a         5623 c         5721 g         5201 t
ORIGIN      
        1 attttcttct gccagccacg ggggaatagt cccggaaaag aacgcgccat ccgccagtaa
       61 atgaagaaaa tcgccgatca tctcgcctaa ttcagccgga cgtccgttac cttcaccacg
      121 ccagaagggg agccgggcag aacgacccgg agcaggagtc acaatcacct gatcgcgggt
      181 gatttgctgg atccgccatg aggtagcgcc gagagtgata atgtcgttca cccgcgactc
      241 atataccatc tcctcatcca gttcacccac ccgccgcgaa ccggcctttt cttcaccttc
      301 gggtaataac acgctataca tgccacgatc cggaatggtg ccgccgctgg taaccgccaa
      361 caattgagcg ccaggtcggg cggtcaatat cccggtctcc ctgttccaga ccagtttggg
      421 gcgaaaagca gaaaaatcgc cagagggata gcgcccggaa agcatatcca gcgtggcgtc
      481 aaaaacacgt cttggcagat ctttccacgg tgcggcacgg cgtacgcggg agtaccattc
      541 gtctacctgt aatgcatcca tcgccgcggc ggcaacggtt tgctgcgcaa ggacgtcgag
      601 aggattatgc ggtggtgtca ggttttccag cctgcctgcg aacatacact ctacaatgac
      661 tgcggaatcg actaaatcac gccgggtacg ggggaaaaac agccctttag atacaccgcc
      721 aacctgatgt ccggcgcgac caatgcgttg taacccactg gcaacagaaa gcggcgttgc
      781 ctcctgaatc accagatcca ccgcgcccat atcaatcccc agttcaagac tggaggttgc
      841 gaccacgcag cgtaattcac ccgatttcag cgcctgttcg gtgattgctc gttgttcttt
      901 agagacggag ccgtggtgtg agcgggcaat aaaaacgtcg ctactttgta cacggttaga
      961 ggttgcgccg gaggtcgact cgaaatgggc cgcatcaacg gcgatagacg gggaacgctg
     1021 taagcgtgcg gcgtaaagct catttaatcg tgccgtcagt ttttccgcca gcccgcgtga
     1081 attagtaaag acaatggtcg agcgatggcg caacacttca tcaaggatac ccgtttcaat
     1141 atatggccag atggagcctt cccggccggc atggctgtct tcgccggtgc cgctggcgac
     1201 cgatgagaca tcatccatat tggcgaccgg tacgacaatt cgtatctgcg gatggcgcat
     1261 tgcgggcggg ttgactaccg taaccgggcg atcgccacca agaaatgctg ccacatcgct
     1321 ggctgagcgc acagtggcag aaaggccaat tcgctgtgct gaggtgtgga gcagcgcatc
     1381 gagccgctcc agacttaacg ccagatgcgc accacgttta ctgcccgcta ccgcgtggac
     1441 ttcatcaata attaccgttt cgacgccgtg tagcgtttcg cgcgcgcggg aggtcagcat
     1501 cagatagagt gattcgggtg tggtgatcag aatatccggc ggattacggg tgagtttgct
     1561 gcgttcctgt gcaggcgtat cgccagtacg gatccctacg cgaagattga cttccgtttc
     1621 gccgcgccgc cgccgttcat cggcaatacc cttcaacggg atctgcaagt tgcgctgaac
     1681 gtcggtgccc agggctttta tcggtgaaat atagaggatg cgtgaggttt tacgcttatg
     1741 cgcctcgcgg gtatcttcgc cgccttcgcg gaagagccga tcgagggcgt agagaaatgc
     1801 tgccagcgtt ttcccggagc cagtcggtgc aatcaccagc gcatgttcgc ctcgcgccgc
     1861 cacatgccag gtttgcgact ggacagcggt cggctgttta aaggcgcgaa gaaaccagtc
     1921 gcgggtcgcc ggtgaaaaca cgtccggcag gagcgatgaa gggtctggat tatctgccat
     1981 taacgctatt ccccgtgaat atcatgcttg atgcgtggaa tagcctcact atagatgcct
     2041 gaatcattgc atgtcatcag gcatcgactc gattacacct cttcggcggc aggtagcggc
     2101 cagcctccca gacgtttcca gcggttgacg atttcacaaa acagcacagc agtgcgttcg
     2161 gtgtcgtaca gcgcggagtg cgcctgggtg ctgtcgaagt ccatgccagc ggtctggcaa
     2221 gcctttgaca atacggtttg tccgagtgcc agcccggcca gtgcagcagt gtcaaaagtg
     2281 gcgaaagggt ggaacgggtt acgtttcagc gaggcgcgtt ctgcggcggc catcataaag
     2341 ctgtgatcaa aattggcgtt gtgcgccacc ataatggcgc ggttacagcc gctcgcttta
     2401 atacctttac gtacaacttt aaaaatttcg tgcagcgcct cgtattcgct gaccgcgccg
     2461 cgatcgggat cgttcgggtc aatgccgttg aaggcgaggg cttctggttg caaatttgcg
     2521 ccgacaaatg gttcgacgtg gaaatgtaat gtggtgtccg gcatcagcca gccttgttca
     2581 tccattttca gggtgatggc ggcaatctca agcagcgcat cggttttggc gttaaatccg
     2641 gctgtttcaa catcgatcac aacaggataa aaaccacgaa aacggtcgca cagaccggta
     2701 agttgagcgt tatcggacat ctgattctct taatacggat aaattgcagc gcgcattatg
     2761 acaaatatta cttgtcgatg cagtaaagat gcgggcgcga tgagttcacg cccggcagga
     2821 gattagttgc ccagaccgcg accggcgtct ttctcttcga ttaactcaat tttgtaaccg
     2881 tccggatctt ccacaaacgc gataaccgta gtaccgcctt ttaccggacc cgcctcacgg
     2941 gtcacgttac ccccgttttg acggattttt tcgcacgctt cagcggcgtt atctacgcta
     3001 agcgcgatgt gaccataagc agtgccgagt tcgtatttat ccacgcccca gttgtaggtc
     3061 agttcaatca ccgcttcttc ggtttccggg ccgtagccaa caaacgccag tgagtatttg
     3121 tattccgggt tttcgctggt acgcagcagt ttcatgccca gcactttggt ataaaaatcg
     3181 atggagcgtt gcaaatcgcc aacgcgcagc atggtatgaa gaagacgcat ctttttatcc
     3241 tcaaaatggc taacagattc aaaatgttgt tttagtatag cggcggcttt acgccgctcg
     3301 caatgttgga ttacaacgtc gggtaatcgg tatagccttc cgcgccgcca ccgtagaaac
     3361 tttcggcacg ctgtgggtta agctcagctt tgagctgcaa gcgggcgacc agatccgggt
     3421 tcgcaatcca gtcacgacca aatgccaccg catcaattaa ccctttgccg atcagcgttt
     3481 cagctttttc tactgtgtat gcacctgcgc cgataatcgg accgtggaaa cgggcgcgta
     3541 ctttttcgcg gaacgcatca gtatacggtt caccccccgc ccaatctggt tctgacatat
     3601 gcagataagc aatgccgcgt ttacccagtt gttcaatcag atacagtgca tcggcttctt
     3661 cattcgggcc gttatctgtg ttctggaaag taccgattgg cgaaacgcga atgccaatgc
     3721 gatcggcacc ccattcttca atcccggcat cgaccacttc cagtaccaaa cgtgcgcgat
     3781 tttccacgct gccgccgtac tgatcggtac gatggtttga agaaggggaa aggaactgat
     3841 gcagcaaata accgtgagca gagtggagct ctaccagatc aaaaccggct tcacgcgcgt
     3901 tagcaatggc ctgacggaaa tcattgacga tacccggaat ctcttccagt tcaagcgcac
     3961 gcggcatgga tgtttcaacg cggatcgccc gaccattttc atcgcgcaga gaagtacgtg
     4021 ttcccgcgct aagtgctgaa ggcgctaccg gtgcctgacc gccaggttgc aggctggcgt
     4081 gagaaatgcg tccggtgtgc cacagctgca aggccatatg accattttca gcatgaacgc
     4141 cagcggtgat ttttttccat gcggcaattt gctccggact atggatgcca ggcgcacctg
     4201 catatccttt tgcctgggca gaaatttgcg tggcttcact aataatcaaa ccggcactgg
     4261 cacgttggcg atagtattcc gccatcaacg gggtaggaat gtcacccggt tcaatactgc
     4321 gcagacgcgt cagcggtgcc ataaaaatac ggtttgccgc cgtgatcgcg cccactttca
     4381 gtggggaata cagtttttca gatgacataa aggctcccgg tcgtctggtg ataaaaaatg
     4441 cctgctaaac ggcaggcgtc gcaataatgt tttttacatg ggccagcgcg ttttccagtg
     4501 gctcgaaact gcgcgaaatt ttggcctgca gattcgcgcc aagccatagt gcgtaaagca
     4561 cttgtgcctg ttgtagcggt tcgccacaaa agattaaaca atggttctca cggccatttt
     4621 ccagagcctg cgagagcagg gcgatcacgc cgcgagcacc tttatccatc gcgctgcgca
     4681 tatcttctga cagatcgcac acttcggcag agagttttac tgtcaggcaa ccactgatgg
     4741 ttccatgttg gcaaaactgg ttcagtgttt gctggtaata agccagtatg cggtcgcggt
     4801 agttaccttc gccggattgc agcaactcag ccagtcgctg gtgatatgcg gcgtaatgac
     4861 gctcaagcat ggcaacgcca aacgcttctt tagagcgaaa gtagtgatag aaggaccctt
     4921 tcggcacttc agcggttttt agtaattcgc ttagccccat cccggtgaat ccacgttgca
     4981 ggcaaagttg ctcgcccgtc gccaagagat gttcgcgagt atcatgttcg gtgtgtttgt
     5041 tcatgcgttg gagtgtagta gaccagtcgg tctagtgcaa gcaaagttgc tactttggac
     5101 attaacagct tcaataatgg taataatctt atctgaggag ggcgaggtgg cggagcaatt
     5161 agagttcttt cccgtccaga gcccgtgccg gggaatttgc cagtctgacg aacgcgggtt
     5221 ttgtcgcggc tgttttcgta gtcgggatga gcgttttaac tggaataaaa tgagcgatgg
     5281 cgaaaaacaa gaggtgctgc gcctttgtcg gcagcgatta atgcgcaaat tacgcgcaaa
     5341 taagccagcg tcatcagacg aaccggagca accatcactc ttttgaacct aatcttgcgt
     5401 atactcctct catatctgat aagaggaagc gattatggtt cagcgtatta ctattgcgcc
     5461 ggaaggcccg gagttttccc gttttgtgat gggctactgg cgattgatgg actggaatat
     5521 gtccgcccgc cagctggtca gttttattga agagcatctg gatctcggcg tgaccaccgt
     5581 ggaccatgct gatatttatg gtggctatca gtgcgaagcg gcgtttggcg aggcactgaa
     5641 actggcacct cacctgcgtg aacggatgga aatcgtcagt aaatgcggta tcgcgacgac
     5701 cgcgcgtgaa gaaaacgtca ttggtcatta catcactgac cgcaatcaca tcattaagag
     5761 cgccgaacag tcgctaatta atctcgcgac cgatcatctg gatctgctgt taatccaccg
     5821 accagacccg ttaatggatg ccgatgaagt ggcggacgcg ttcaaacatc tgcatcagag
     5881 cggcaaagtg cgtcattttg gcgtatcgaa ctttacgcct gcgcaatttg ccctgttgca
     5941 atcacgtctg ccgtttaccc ttgccactaa tcaggtggaa atatccccgg tgcatcagcc
     6001 gttactgctg gatggcatgc tcgaccaact acaacaactg cgtgttcgtc cgatggcgtg
     6061 gtcctgcctt ggtggtggtc gtctgtttaa tgatgattat ttccagccgc tgcgtgatga
     6121 actggctgtg gtggcggagg agttaaacgc gggctcgatt gaacaggtgg tttacgcctg
     6181 ggtattacgt ttaccgtcgc agccgctgcc aattatcggc tcaggtaaaa ttgagcgcgt
     6241 acgggcagct gtcgaagcag aaacactgaa aatgacccgt caacaatggt ttcgtatccg
     6301 taaagctgca ctggggtacg acgtaccgta agcactttta ggaatagccg ccgttcaaaa
     6361 atgtgtcact ggtttacact tattcaggaa tgcacaatga acggaggtcc tatgaaacgt
     6421 tttagtctgg ccattctggc gctggttgtt gcgaccggcg cacaagctgc cagtgaaaaa
     6481 gtcgagatga acctcgtcac gtcgcaaggg gttgggcagt caattggtag cgtcaccatt
     6541 actgaaaccg ataaaggtct ggagttttca cccgatctga aagcattacc ccccggtgaa
     6601 catggcttcc atattcatgc caaaggaagc tgccagccag ccaccaaaga tggcaaagcc
     6661 agcgccgcgg aatccgcagg cgggcatctt gatccacaaa ataccggtaa acatgaaggg
     6721 ccagaaggta ccgggcattt aggcgatctg cctgcactgg tcgtcaataa tgacggcaaa
     6781 gctaccgatg ccgtcatcgc gcctcgtctg aaatcactgg atgaaatcaa agacaaggcg
     6841 ctgatggtcc acgttggcgg cgataatatg tccgatcaac ctaaaccgct gggcggtggc
     6901 ggtgaacgct atgcctgtgg tgtaattaag taattacgag gccagcgtac cttgcggtgg
     6961 tgcttgctca agttgcgaaa gcgagcagta cagccgccag acaattgccg ccagctcacg
     7021 ggcggcaggt tgatgatgac gggcaaggct gtcgcaaatc cgctgtaatt cttcaagtgt
     7081 ggccgccagt gatttttgct gaacgccacg ctcactcatc actccccgca acagtgaaat
     7141 acagttatcc cgtactcgcg ataacggatc ggaacgcgat tcccagtcgc gcaattgcca
     7201 gacaacatga gaacagttca gcagcactac accccagcgt aataaccaac ggcgagccag
     7261 cgcatcctgg ctgttactca actgactgac gtgatgataa gtgagcgatt caaattcgct
     7321 ttcactcagt gttggatggc ggcttagctg atcgacaaaa tcccggcgca gcgcgcgaat
     7381 atggcggcgg cttttacgag catccgatcc tggacgcaga atggcgaacg ctagccaagc
     7441 cagcgcaacg ccaacgattt ttgccagatt atcgttaaga aaatcagcaa aatcatacac
     7501 cgggggatta gtgacagcga taaacgaacc cataaaaaca atcagttgcc cccacagtgc
     7561 ggcaaatttg ggcatctgca atttaagaag ctgcattgtc gccagcagtg gaaagagaaa
     7621 cagtaaaaat tgccacagat cgctaatctg gaccatcagg ccaaatttga ccacaaagct
     7681 gaatagcgaa agtaacacca gcgtgcgcat cagcagtgac aacgacttaa acggtgctgc
     7741 gacggcggag tagagtacgc agctaattgc tgccagcgtt aatgcattgg caccggcatc
     7801 ccattgcgat gcaatactcc atgcgccaat catcatcaac gtacaaaatg tacgcagccc
     7861 gctccacata gcttcggcgt tgtcggtatg acgagccaga gcggatgtcc gtgcgagtct
     7921 ggcatgatca tctacaccgc tttgcagacg atgtaattcc tgactacttt gcagataaag
     7981 gcggcaaaaa taacgtagtc gctgccagaa ggcgacgtgc cggtagtcgg cgacgttggt
     8041 cgggcgtagc ggggcgataa tacgtgcgac ggtgtaagca tctgtttgcg aactggcgag
     8101 cgccgtcagt aattgttcga gaatttctcg cgtggcacct ggcggtgagg gccagttgag
     8161 caacatacgt cgcaggctgg agatgacact ggtcatacgt aattgctggt ggagtagcgc
     8221 attaaggcgc gcgttttgct ggcgaaaacg atagtggctc cagaaagcct ggatacgcag
     8281 caaattcatg gtcagtatct gcccaatcac cccctcatgt gctgcacgaa tggcatcgtt
     8341 tgtttcaggc tgccagagta aactggcatg ttccagtaat cgggcgtgca tgtttttcaa
     8401 tgcagttaaa agggcagtag catcggaact gctaggcagg atcatcatca tcatgccgcc
     8461 gcacaaaata ccgactatca cctcgcaaac gcgcgcctga gcgatatccc acagctggct
     8521 ggcctcagta atattaacca tcggaaaggc gatgatggca gccgtgtagc ccgccagttg
     8581 aaatgcatac gcgacgttat tcgtgaagtg cgcacaggcc taggtacaaa agccaagcca
     8641 cgccgacatg ctcaatagaa aaaaccacgg ctcattgagc gtatgtccgg caagaagcag
     8701 tgccgcaatg gctcccagta aactgccagc gatgcgtccg aggcttttgc tgatgacacc
     8761 gccaacggtg ggaaagctaa ccactgcagc cgaggtcatc gcccaatagg gttcatccag
     8821 attcaaataa taggcaaccg tcagcgccag acacatggca atggtattgc gtaacgcata
     8881 acgccattgc gccagcgtgg ccctgaacca gcgcaaagcg gacgggaacg cgttgggcga
     8941 gacgaaccca ggggacgtta ggtttcacat ccggaataag gcttgagtca ctttcaacgc
     9001 tctgatcata aatcgcccga ccgatactgg aaacgtgacc ctgtaacgtt ttgttatcac
     9061 tatagagggt aatttgtgct ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa
     9121 aataaccaat gacgtaaaac gagtggctgt cgacaagggc aaacagcggt ttcccggtgt
     9181 cagcgtaatc gccgatgcgg gtagtgaggt tagtcaccca tccactcacc ggggcgcgaa
     9241 tttccgtttg cgccagttgc cattgtgtct gtttcagcgt cgcctgtgcg gcattaacac
     9301 tggcctgcat cgctttaaca ttgaggttag cggtatcgag ttcttcggca aaaataaaat
     9361 tttgtgagag atgacgacga cgattcgctt cgttattggc ttttgcgagg tcagattgcg
     9421 ctttcgccag ctgagcctgt gcgttcagct cggcgatctg aaagggcgtt ttatcgatcg
     9481 tgagcagaag ttcccccgcg ttaaccagtt ggttgtcttt tatattcagc tcaacaatac
     9541 gtcccgacac ctgtggggtg atagaaacct gttctgcgtg tattttcccg tcgcgtgtcc
     9601 acggagattg catgtaataa ttccacagcc accatccggc aagaaccgca actatcgcta
     9661 caatgattgt tgagaaatac ttaattgttt taattgacat agttaccacg caatcagtat
     9721 tgccagagca aggcaaacgc aaatcgcaaa cagcgataaa tccattaaca agggatgcca
     9781 gatgtcaccg gcgtagatcc agccacgaag cagacggtgt acgacaagcc agatgacgaa
     9841 tccaaacgcg aatgctttga aaaacggagg aaaatagacg gacgcaccga acaccagatc
     9901 ttgtaagggc aatcctgttg cattgagcat aaacttcaca aagtagattc ctttacgacc
     9961 gaatatattg cgttagatta ataaatattc tttaagtgcg aaaaatttac gcgcaatttc
    10021 tgaaaaatat gtctgacggt aaccaaatgc agcaatacat ttgttttagc aatacaattg
    10081 ctgcacacta ttctaaaagc cgcataatat cttagcaagc taattataag gagatgaaat
    10141 tggaatcgcc actaggttct gatctggcac ggttggtgcg catatggcgt gctctgatag
    10201 accatcgcct gaaaccgctg gagttaacac aaacccattg ggttacgtta cacaatatcc
    10261 atcagttacc tccagaccag tcgcaaattc aactggcaaa agcgattggc atcgagcagc
    10321 catcactggt ccgtactctg gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt
    10381 gtgccagcga tcgtcgggct aaacgtatta aactgacgga aaaggcagag ccactgatca
    10441 gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat attacatggc atctccgcag
    10501 aggaactgga gcaactgatt acgctcatcg caaaacttga gcataatatc attgagttac
    10561 aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat
    10621 ttagcgcgga gaaacggtca cctgactgcc attgctggcc agtacgacac gttggcccgg
    10681 agagaaacga gtgttgcctt gtttctgtac caccatgatg gtattaccat cgtctttacg
    10741 aatttccagc tcgacaccct gcgttttgtt cattgcactc tgtacgccct gaccagctac
    10801 gccacctgca acagcgcctg ctgcagtagc cagagaacgc ccggttccgc caccaacagt
    10861 attccccagg aaaccaccaa gaacagcacc gccaattgca ccgataacgt tggaatcatc
    10921 accgccctga atctgtaccg gacgtacgtt aacgatggtg ccatagctga cattctgtac
    10981 ttgtttcgct tcagaagcgg tataaacatc ccctgacagg gtgtcgttat taacacaacc
    11041 gacaagagac agacctacca ttgaaacaac caatacgcgt ttaatcactg aaaaatctcc
    11101 tgttcaccat gaaacgccac gcaagcatcc ctcatggtca aagtatatgg catatgtgaa
    11161 tccatggtta catgttctac caaatatgag caaatcataa tgaaagtagg atcaaccaat
    11221 tataaacaaa acattcatga tgattattga tttgaagata atgttttact accaacggta
    11281 aaaatcggtc attccgcatg gtattcacga aaagtttatg tttgagtggt gcgcaaacgt
    11341 cagattgaaa aaggatgaac tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc
    11401 agccttgatg gtgttgatgt tgtgttggcg acaattgatg aacaccgggt cgcacagctg
    11461 gcaagcttga gctggccgat cccggtgtct ctgaaacagg ctgtactgga tatttgccag
    11521 ggccagcagc ttacactttc gcagtttgga cagcttgata ctcaactcgg gcgacttttt
    11581 gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc aggcgagaga tatagttgcg
    11641 atcggctgtc acggtcaaac cgtctggcat gaaccgacgg gcgtggcacc acacacttta
    11701 cagattggcg ataacaatca aattgtggca cgcaccggaa ttacggttgt cggtgatttt
    11761 cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc tggtacctgc gttccatcat
    11821 gccttgctgg ctcacccaac cgagcgacga atggtgctca atattggcgg catcgccaat
    11881 ctgtcactgc tcattcctgg gcagccggtt ggcggctacg ataccggtcc tggtaacatg
    11941 ctgatggatg cctggatctg gcgtcaggca ggtaaacctt acgataaaga tgccgagtgg
    12001 gcacgggcgg gtaaagttat tctcccactg ctgcaaaata tgctcagcga cccgtatttc
    12061 tcgcaacctg caccgaaaag caccggacgc gaatacttta actacggctg gctggagcgc
    12121 catttacgcc attttccggg tgttgatccc cgtgatgttc aggccacact ggcagaactc
    12181 accgccgtga ccatttctga acaagttttg ttgagcggtg gctgcgaacg attgatggta
    12241 tgtggtggag gtagtcgtaa tccgctactc atggcgcgtc tggcggtatt actgctaggc
    12301 acagaagtca ccaccaccga tgccgttggc attagtggcg atgacatgga agcattggct
    12361 ttcgcctggc ttgcctggcg gacgctggcg ggattaccag gaaatctgcc ttccgtcact
    12421 ggcgcaagcc aggagacggt actgggggct attttccccg ccaactcgtg atatcagaat
    12481 taactgaatt ttttcttatc gtgtctgccg ttacacttgg aagattaagg agggtatttg
    12541 ccctccggac caggaaagtc ttcgggatat gactatgaaa aaactgttaa ttatcatttt
    12601 gccggtcctt ctctccggtt gtagcgcctt taatcaactg gttgagcgaa tgcaaaccga
    12661 tacgctggaa taccagtgtg atgaaaaacc gttgacggtc aaactgaata atccgcgcca
    12721 ggaagtcagt tttgtttacg ataatcaact actgcatctc aaacagggct tttcagcctc
    12781 tggcgcgcgt tactccgacg ggatctatgt tttctggtcg aaaggtgagg aagcgactgt
    12841 ctataaacgc gaccgcatcg tcttgaataa ctgtcagtta caaaatccac agcgttgaga
    12901 tttttccagg ggcggcgcac aatagcgtca cccactgaca atccgtaaag aaaaccatgt
    12961 ctgataacga cgaattgcag caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt
    13021 tacgccgccg cgatcttccc gccgatccat taaccctttt tgaacgttgg ctctctcagg
    13081 cttgtgaagc caaactggcg gaccctaccg cgatggtggt cgctaccgtg gatgaacatg
    13141 gtcagcctta tcagcgcatc gttttactca aacattacga cgaaaaaggc atggtgtttt
    13201 acaccaacct cggtagccgt aaagcacatc aaattgaaaa taatccgcgc gttagcctgc
    13261 tgttcccgtg gcataccctt gaacgccagg ttatggtgat cggtaaagca gaacgacttt
    13321 cgactctcga agtgatgaaa tattttcata gccgcccgcg tgatagccag attggtgcat
    13381 gggtttcgaa gcagtccagt cgcatttctg cccgcggtat ccttgaaagt aaattcctgg
    13441 agctgaagca gaagtttcaa cagggcgaag tgccattgcc gagcttttgg ggcggttttc
    13501 gcgtcagcct tgaacagatt gagttctggc agggcggtga gcatcgcctg catgaccgct
    13561 ttttgtacca gcgtgaaaat gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg
    13621 caaaaatctt gctttaatcg ctggtactcc tgattctggc actttattct atgtctcttt
    13681 cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca tggagatttt
    13741 gatggcaagc agtaacttga ttaaacaatt gcaagagcgg gggctggtag cccaggtgac
    13801 ggacgaggaa gcgttagcag agcgactggc gcaaggcccg atcgcgctct attgcggctt
    13861 cgatcctacc gctgacagct tgcatttggg gcatcttgtt ccattgttat gcctgaaacg
    13921 cttccagcag gcgggccaca agccggttgc gctggtaggc ggcgcgacgg gtctgattgg
    13981 cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg
    14041 ggtggacaaa atccgtaagc aggttgcccc gttcctcgat ttcgactgtg gagaaaactc
    14101 tgctatcgcg gcgaacaact atgactggtt cggcaatatg aatgtgctga ccttcctgcg
    14161 cgatattggc aaacacttct ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg
    14221 tctcaaccgt gaagatcagg ggatttcgtt cactgagttt tcctacaacc tgttgcaggg
    14281 ttatgacttc gcctgtctga acaaacagta cgatgtggtg ctgcaaattg gtggttctga
    14341 ccagtggggt aacatcactt ctggtatcga cctgacccgt cgtctgcatc agaatcaggt
    14401 gtttggcctg accgttccgc tgatcaccaa agcagacggc accaaatttg gtaaaactga
    14461 aggcggcgca gtctggttgg atccgaagaa aaccagcccg tacaaattct accagttctg
    14521 gatcaacact gcggatgccg acgtttaccg cttcctgaag ttcttcacct ttatgagcat
    14581 tgaagagatc aacgccctag aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca
    14641 gtatgtactg gcggagcagg tgactcgtct ggttcacggt gaagaaggtt tacaggcggc
    14701 aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt gcgctgagtg aagcggactt
    14761 cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg gaaaagggcg cagacctgat
    14821 gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt caggcgcgta aaactatcgc
    14881 ctccaatgcc atcaccatta acggtgaaaa acagtccgat cctgaatact tctttaaaga
    14941 agaagatcgt ctgtttggtc gttttacctt actgcgtcgc ggcaaaaaga attactgtct
    15001 gatttgctgg aaataatgca ttaagtggaa agggggagtg agaaatcact ccccctggtt
    15061 tttatacagg gaacatgatg aaaaatattc tcgctatcca gtctcacgtt gtttatggtc
    15121 atgcgggtaa cagtgcggca gagtttccga tgcgccgcct gggcgcgaac gtctggccgc
    15181 tgaacaccgt tcaattttct aatcacaccc aatacggcaa atggactggc tgcgtgatgc
    15241 cgcccagcca tttaaccgaa attgtgcaag gcattgccat tgccgccatt gataaattac
    15301 acacctgtga tgccgtatta agtggctatc tgggatcggc ggagcagggt gaacatgtcc
    15361 tcggtatcgt ccgtcaggtg aaagcagcga atccgcaggc gaaatatttt tgcgatccgg
    15421 taatgggtca tccggaaaaa ggctgtatcg ttgcaccggg tgtcgcagag tttcatgtac
    15481 ggcacggttt gcctgccagc gatatcattg cgccgaatct ggttgagctg gaaatactct
    15541 gtgagcatgc ggtaaataac gtcgaagaag cggttctggc agcgcgcgaa ctcattgagc
    15601 aagggccaca aattgtgttg gttaaacacc tggcgcgagc tggctacagc cgtgaccgtt
    15661 ttgaaatgct gctggtcacc gccgatgaag cctggcatat cagccgtcca ctggtggatt
    15721 ttggtatgcg ccagccggta ggtgttggtg atgtgacgag cggtttactg ctggtgaaac
    15781 tgcttcaggg ggcaacgctg caggaggccc tggaacatgt gaccgctgca gtctacgaaa
    15841 tcatggtgac caccaaagca atgcaggaat atgagctgca agtggtggct gctcaggatc
    15901 gtattgccaa accagaacat tacttcagcg caacaaagct ctgaaatatc tcatttcggc
    15961 ccacaacgac ctgtgggccg atggctatta ctttaagcct tccgctgaca gcgcgtcttg
    16021 tacttccgga cgttcagcca tacgttgcat aaatgccgca atgtgctcta acccttccag
    16081 attcagtttc accgcgtatg cccagcgcag aaccgtaaac agataggcat cagcaattgt
    16141 aaatctttgc ccgcagatcc agtgctcatc cttcagtgcc tcgttcacat attgcagctt
    16201 cttatccagc tgcgcgcgaa ctgtcggttt gtactcttcc ggtgtatctg ggcgaaacag
    16261 aggtgtgaaa cctttatgca gctcggtggc gatgtaattc agccattcga tggttttata
    16321 gcgggaaata ctgtttaccg gtgccagcaa ctggcggtcg gggacgctgt cggcaagata
    16381 ctgcataatc gcaacgcctt ccgtcagcaa agtaccatcg tccagcagca atgcaggcac
    16441 ctgcccctta gggttaacgg aaaagtaatc gtcaccgttt tcgagacgtt ttttcattaa
    16501 atccacactg acgagcgtaa aatcctttcc gctctcacgc agggtgatat gggaagcgag
    16561 agagcaggcg cccggtttgt agaacaattt catcggtaac tccttttgtc tgtggtttaa
    16621 agtatggtag tgcgttgctg aacaaaaaaa aagccgctaa cgaaaagtta gcggcttgta
    16681 aaagagtttc cctgaatttt acgctacagt agctttcgcc gctttgtctg cagcgtcatc
    16741 ctgcgtcatg cggtgcagtt taggcgcggt cagcagcatc agtactgcaa taacggcagt
    16801 agcgacacca atctgcaaga atacgcgacc atagacttcc agtgacatca gcgggtcggt
    16861 aacgttatcc ggcacagcca tcatacccgc aacataacca ccaatcaggt ttgcaccggc
    16921 agtggtcagg aaccagctac ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc
    16981 aaccattgcc agacccagac cagagatcat cagttccccg atgctctgca ggccatagct
    17041 tgcgaccagc cagctgacag acacgatacc tgcgtcagac gcgaatttcg cacccagcgg
    17101 cagaatcagg aacgcaccag aacacatcac catgccgatt gcaaacttgg ttggcatcgg
    17161 cagggtatca cccatcttgt tatagatagc ggccagaatc ggactaccga tgatgatcca
    17221 gaacgggttc agtgcctgat actgctcagg ttctacggcc agacccagaa tggagtgctc
    17281 aacgttacga atcgcaaaga agttcagtga cgttggcatc tggctgtaca gcacgaagaa
    17341 gatgattgct tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt
    17401 catcgcgaaa gcttctttac cgaagataac cacaataccg aaggcaacaa cgcccagcgc
    17461 catacgcgca acttcctgat tgtgcagtag ccaggtggca acagcgatca gcgccacaac
    17521 accaataatg gtcagcaaca ggttacggta gttgactggt tcaaagtctg gttttgaacc
    17581 gtactgttta acccagcgtt ggcagaaggc gaagttaacg atagtgatca gcaggcctac
    17641 aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg gccagccacg gggtggctat
    17701 catagagaag aaagagccga tgttgacgga catgtagtac atggtgaatg caccgtccag
    17761 acgtgggtcg tttttctcat agcatgtaga aagcagagaa gacgggttag ctttaaacag
    17821 gccgttaccg accgcaatag ccgccatacc catataaaca ataccggcgt cgtgaccaga
    17881 ccaggcaacc agagcataac caatcgccag cacaatagcg ccgagcataa ttacgcgttt
    17941 agtacccagt accttgtcac ctaaccagcc gccgatagcg accagaccat aaaccagggc
    18001 actaaaggaa gagaaaaggg tgattgaatc cgcttcagac atacccagtt gtttaaccag
    18061 gtaaacagcc ataattcctt gtaggccgta ataaccaaaa cgttcccata actcaatcga
    18121 gaagatgaga tagaacgcct tcggttgttt gaaagcgttc aaactgacgc tttcagttgg
    18181 tttttggttt gcagtggaca cttttacctc tttttttaca tcccatatta acgggggggc
    18241 tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta tagtgggagg ggaaacggca
    18301 ggtaatgttc actatcttgc actttcagac aagtcctttg taataatctg ttacatataa
    18361 ctgaggcggg gaattcaacc attccgggaa atgttatcca gcgttaaatt tttcaaaaaa
    18421 cgtaacggca gattttttca ctgcattgag agggggatga tggctttacg gagatatgtt
    18481 ctgctattta gcgtttttcc tagtgagata gtccagtttc tgaaaaatag ccagtgtaat
    18541 gttttgcagg tcaatatttg ttgcgtgtgt aaatttaaat cattttaacc tgattcttgc
    18601 aatgttttta cattgccaca gcatcgtgat cttgatcacg aatgaataga aaatttcgct
    18661 attaaaaaaa caattaacct gcatttttgg ttatccgcaa acggattacc tgatgataac
    18721 gctaagcaat ggcatcattg acgttaaaaa gatgttttaa aagaggataa agaaaggtta
    18781 tcaatggggt aatcggtgtt accccttttc ttcagatgtc aactctctct ttgtattcac
    18841 aaagatcttc aataatacaa gagccacagc ggggcttgcg ggcaatgcag gtataacgcc
    18901 cgtgcaggat caaccaatgg tggcagtcga ctttaaactc tgccggaacc actttcagta
    18961 gcttttcttc tacctgttcg acgtttttcc ccggcgcaaa ttgagtacga ttacaaacgc
    19021 ggaaaatgtg cgtgtcgaca gcaatagtcg gccagccgaa tgcagtgttt aatacgacgt
    19081 tggctgtttt acgacctacg ccgggcaggg cttcaagcgc agcacgatct tccggaacct
    19141 cgccattatg ttgttccagt aatatacggc aggttttgat gatattttct gctttgctgt
    19201 tataaaggcc aatcgttttg atataggttt tcaccccttc aacgcccagt tcaagcatcg
    19261 ctgcaggcgt attcgccacc gggtagagtt tcgccgtcgc cttattaaca ctgatatcgg
    19321 tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa aggcgaactg aaattaagct
    19381 cggtggtggg atgaggattg ttctcacgca ggcgagtgag gatctccaga cgttttgctt
    19441 tgttcatcag acattccctg tttcaccgtt tggcaatgca cgttcggcag ctgcttcagc
    19501 acggcgcttt ttcattcttt catcaatcag gtattttcct gccagcatca gtcccaagcc
    19561 aataaatgca cctggtggca gcatcgccag caggaaaggg gagtcggtgt ggaaaatctc
    19621 cacgcgtaat acttttgccc agctacctaa cagcgcatct gcaccgtcaa acaacgtgcc
    19681 attgccgata atttcgcgta gtgaacccag cacgaacatg gcgcaggttg cgcccgtacc
    19741 aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt ttggcggcga aggcttcagc
    19801 gcggcccaca acgatacagt tagtgacaat cagcggaata aaaatcccta atgattgata
    19861 caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg ctgaccaccg aggcgatgat
    19921 catcacgtaa atgggaatgc ggatctcggc tggcgtccag tgacgcagcg tcgaaatggt
    19981 caggttggtc agcgtcagta ccagcgtagt cgcaagtcct aaacccagag cgttagtggc
    20041 agtggacgtg accgccaata gcggacaaag gccgagcagt tggaccagtg cagagttgtt
    20101 tttccacaac ccctgaacaa taacgtcttt aatttcgctc acgctttatt ctccacaggc
    20161 aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac aatccggcgc gttttaccgc
    20221 attaaccacc gcgcggggag taatcgtcgc gccggtgaac tggtcgaaat caccaccatc
    20281 tttcttcacc gcccagtgcg catcatccgc accactgatt ttttttcccg caaaatgggt
    20341 gagccagtca gaaaggcgca attcgatttt atcgccaagc cctggcgttt cgtggtgctc
    20401 tgtcacgcgc gtgccaagta ccgtgccatt aaaatcggct cccaccagca gctgaatcgc
    20461 accggaatag ccatctggcg cggttgcttc cagaacggcg gctaccggtt tgtcatcctg
    20521 tttggcgatg taaacccgat gctcaccttt acctaactct ggcgcagtta ccagatagca
    20581 actctgtgcg agcgcattgt tatagcgttc ggctggcagc acctgaccaa ataacgcctt
    20641 ttgttgcaga ctggcctgtt cagcaattgt cgttttggtc atctggttga tggccgcagt
    20701 taaccctgta gaacccgctg caaatagtgc cagtgtaatg ccgtgttttc ggatagtttt
    20761 cagcatggtt taccctttgc gatggccgta gacgcgcgga cgcgtgtagt aatcgatcag
    20821 aggaaccgtg atgttcgcca gcaggacggc aaaagccacg ccgtcaggat agccgccgaa
    20881 actgcggatc aaccagacta ataagcccgc cagcgcgccg aacatcagcc gaccacgatt
    20941 ggtcgtagaa gcagtaactg ggtcagtcaa aataaagaat gcgccgagca tagtcgcacc
    21001 agacagcaga tgaatttgcg gtgctgccag tgtttctggt gagaacaacc agcccaacgt
    21061 tgcgcataac gccagcgtta ctaagaagct gaggggaata tgccagcgaa tcgctttctg
    21121 ccatagcaac cacacgccac cagccagcca ggcgagattt acccattgcc aaccagcgcc
    21181 cgccagaata ccgctgtaga tcggatattg cataatctgt tcaaccgaat gacctgcgcg
    21241 gacagaggtt ttgaatgtat ccagcggtgt cgcctgacta atgccatcaa tacctaagcg
    21301 tagcgtattc atatcaccac cactggcggt atgaccgctg aaaataacct gtatggcgtc
    21361 gataaaacca gggatgttga ccgcaatttc atgtggcggt aaccagctgg tcatctgcac
    21421 cgggaaggag atcagtaaga ccacataacc aatcattgcc ggattaaacg ggttttgccc
    21481 cagaccgcca tacaactgtt tagcgatgat caccgcaaac accgtaccca gtacgaccat
    21541 ccaccatggc gctagggggg gaatacttac cgccagcaat aagcctgtca gcaatgctga
    21601 gttatctttc aacgttgcgg ctaccgactg cttgcgtagt ttgagtacga gagcttcggc
    21661 taacagagca ctgaccgacg ccaacaggat ctgaacgaga gtaccccaac caaaaaacca
    21721 cagttgcgct gcgattcctg gcacggctgc gagcaacacc aacagcataa tgcgcgatgt
    21781 ctggcgctgg ttatgggtat aaggggagct agctattctg aataccattt agtcctcgtt
    21841 tacaaccttt tgctgggcgg cttttttggc ctgaacgcgg gcaatagccg cggcaactgc
    21901 ggctttgcgc ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt
    21961 gcgtgctttg gcgcgggcaa tagcggcttc gacggcagct ttgcgcggat caacctgttc
    22021 ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggc
//
LOCUS       DADLZM010000057        22050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLZM010000057 DADLZM010000000
VERSION     DADLZM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.057"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..530
                     /locus_tag="JNY73_002127"
     CDS             <1..530
                     /locus_tag="JNY73_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY8048139.1"
                     /translation="RSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFHECSMHGVK
                     IKPWLPVKWTKELISDYLYGCLLSLYSIGARDIYNMNAGNNVKVAADAFLEIIFSLKN
                     KYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYRPQSQDGE
                     DFRSHLQYKLPLSAH"
     gene            741..1064
                     /locus_tag="JNY73_002128"
     CDS             741..1064
                     /locus_tag="JNY73_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY8048140.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            1061..1891
                     /locus_tag="JNY73_002129"
     CDS             1061..1891
                     /locus_tag="JNY73_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY8048141.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1888..2901)
                     /locus_tag="JNY73_002130"
     CDS             complement(1888..2901)
                     /locus_tag="JNY73_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY8048142.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(3000..4430)
                     /locus_tag="JNY73_002131"
     CDS             complement(3000..4430)
                     /locus_tag="JNY73_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8048143.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4441..5442)
                     /gene="ltaE"
                     /locus_tag="JNY73_002132"
     CDS             complement(4441..5442)
                     /gene="ltaE"
                     /locus_tag="JNY73_002132"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY8048144.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5479..7197)
                     /gene="poxB"
                     /locus_tag="JNY73_002133"
     CDS             complement(5479..7197)
                     /gene="poxB"
                     /locus_tag="JNY73_002133"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY8048145.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7330..8298)
                     /gene="hcr"
                     /locus_tag="JNY73_002134"
     CDS             complement(7330..8298)
                     /gene="hcr"
                     /locus_tag="JNY73_002134"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY8048146.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPNLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8310..9962)
                     /gene="hcp"
                     /locus_tag="JNY73_002135"
                     /gene_synonym="priS"
     CDS             complement(8310..9962)
                     /gene="hcp"
                     /locus_tag="JNY73_002135"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY8048147.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10106..11005)
                     /gene="lysO"
                     /locus_tag="JNY73_002136"
     CDS             complement(10106..11005)
                     /gene="lysO"
                     /locus_tag="JNY73_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY8048148.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11462..12157)
                     /gene="aqpZ"
                     /locus_tag="JNY73_002137"
     CDS             complement(11462..12157)
                     /gene="aqpZ"
                     /locus_tag="JNY73_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY8048149.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYAEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            12582..14240
                     /locus_tag="JNY73_002138"
     CDS             12582..14240
                     /locus_tag="JNY73_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY8048150.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(14237..15193)
                     /locus_tag="JNY73_002139"
     CDS             complement(14237..15193)
                     /locus_tag="JNY73_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY8048151.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            15344..16459
                     /gene="macA"
                     /locus_tag="JNY73_002140"
     CDS             15344..16459
                     /gene="macA"
                     /locus_tag="JNY73_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY8048152.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            16456..18402
                     /gene="macB"
                     /locus_tag="JNY73_002141"
     CDS             16456..18402
                     /gene="macB"
                     /locus_tag="JNY73_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY8048153.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(18475..18699)
                     /gene="cspD"
                     /locus_tag="JNY73_002142"
     CDS             complement(18475..18699)
                     /gene="cspD"
                     /locus_tag="JNY73_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY8048154.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            19022..19342
                     /gene="clpS"
                     /locus_tag="JNY73_002143"
     CDS             19022..19342
                     /gene="clpS"
                     /locus_tag="JNY73_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY8048155.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            19373..21649
                     /gene="clpA"
                     /locus_tag="JNY73_002144"
     CDS             19373..21649
                     /gene="clpA"
                     /locus_tag="JNY73_002144"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY8048156.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         5445 a         5523 c         5912 g         5170 t
ORIGIN      
        1 ttagatctgt taaaattaat aacgtaaata ctcattcttt aaaatttatt aactgcaatt
       61 ttttcaatgt tgatatgata agagttaatc tcagcaaatg tctttttcat gaatgtagta
      121 tgcatggtgt caagataaaa ccctggttgc ctgtaaaatg gacaaaagaa ttaataagtg
      181 attatttgta tggctgttta ctatctctgt actctatcgg tgcaagggat atatataata
      241 tgaacgccgg gaataacgtg aaggttgctg cggatgcttt ccttgaaatt attttctctc
      301 ttaaaaataa atattgtatt aaactattaa gcgctcagga tcgggcgttt atatacgagt
      361 ttgcaagaat gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt
      421 gttttgcggc ggctgaccaa aaagcaatcc agcggtatag acctcagtca caagatgggg
      481 aagactttag gagtcattta caatataagt tgcctctttc cgctcattaa tgttgttggt
      541 gcaacataat aagacaggta agcggtaccc tataatgaaa ccaatatcgg gttatgcaac
      601 ggataatcgt aaagacttaa gtgggtgttt ttgacacaat gagatgattt cctaaactga
      661 tcaaaacctt cttcccaaca tggcatatca gtccgataat gctttagaga tcgaaggctt
      721 aacaaacaag gagatcgatc atgcaatttt ctacaacccc aactctggaa ggccagacca
      781 tcgttgaata ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg
      841 atttctttgc cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac
      901 tgcgtaaagc acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg
      961 ccgatgccgt agttggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc
     1021 tgatggttag cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct
     1081 ggtcgctgcc gctctgttac tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga
     1141 gggatatcag cttgataccc gacaccaggc gcaggcggcg tatccgcgca ttaaagtgct
     1201 ggtgatccac tacaccgcag atgattttga tagctcgctg gcgacactga ctgataagca
     1261 ggtcagctcg cattatctga tccctgcggt gccaccgcga tacaacggta aaccgcgcat
     1321 ctggcaactg gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg
     1381 ggcaacgcgc cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa
     1441 atcatccgga gtgaaatatt ttgccccgtt tgaacaggca cagattcagg cgcttattcc
     1501 gctggcgaaa gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc
     1561 ggatatcgca ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc
     1621 gcagcagggg attggtgcct ggccggatgc gcagcgggtg aacttttacc ttgccgggcg
     1681 cgcgccgcac acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta
     1741 tgacgtaaaa cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat
     1801 gcatttccgc ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga
     1861 agcattgctg gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca
     1921 ggcggcagtt ttctcgatgc cttcatccag ggtgatgacc ggctgataac ctaactcttc
     1981 ctgcgcacgc gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt
     2041 cagcggcggc tcttttgctg acttgcggcc taaacgctcc atgctgtggg cgatcatatc
     2101 cagcatcggg taagggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt
     2161 ctgcacgatg ctgcgcagtg tgcgatgctc gccgttggtg atgttgtaca cacgcccgga
     2221 aggtagctta tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg cattttcata
     2281 gtaggtcata tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg
     2341 catcatatgc gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg
     2401 gcgcagaata gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc
     2461 gctggctgct ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt
     2521 aatatcgcga tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt
     2581 acgcacaccc caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag
     2641 atcgaaagcc tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc
     2701 aatgcccgcg agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgtcggaac
     2761 aaactctgcg cccatttttt ccagcaattt acccattgct tcgttgcgac cgctcgcacg
     2821 cacgctgatg cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc
     2881 gccggtaacc agtaccttca tatcaatcca ctgttgttga gaaaatgacg tgcgcattct
     2941 tccgtgattt cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat
     3001 taatctgtgc tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac
     3061 agatgcgcgg ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag
     3121 aaagcgcgga catcgatagt acgatagtca cctttatctt ccagggtaaa acacagtcgt
     3181 cccagccccg gcgctttcat gccaaataac aacgtaagtt gctttttcgg ttcaacgaca
     3241 atcactttcc agctatccac cgcatcgccg gtctgtaaat attcgcgctc cgggcggcct
     3301 ttcgccagat tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg
     3361 ccaaagaaat aacgctcttt accgccgatt tggttcacca ccagccataa agccgcaagg
     3421 ctggcggacg ttttaacggt aaaccccgcc tgtttggcga aataaccgta ctccggtcgc
     3481 cagcgggcaa aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcttcc
     3541 tctttcaacg tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca
     3601 cgtagcgcgg tatcatctgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg
     3661 gtggtgggcg gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg
     3721 agggggatgg ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt
     3781 tgctgataac tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gttggctgga
     3841 tggtctaaca gcgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt
     3901 gaacgtaccc agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact
     3961 tcgaacgccg ctgaacctgc gccaacgata attccggccc gaagttcggt cactggtaca
     4021 ttcgcttcac gaagaatgtc cgccgtagcc tgacgagctc gcagatgatc cgactgctca
     4081 tgtggcgggg cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc
     4141 gcatcgcgga cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc
     4201 atgctgtgca ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc
     4261 ggccagctga gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg
     4321 tcgacatgac gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga
     4381 tgctgaccaa tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc
     4441 ttaacgcgcc aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac
     4501 gtcaagatgc gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt
     4561 catgtattcg cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt
     4621 gtcctgacgc atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt
     4681 gtcgtgatct tcctgcaacc gtgcgacgtt atttttcagg gcatacatcc cggcggcagc
     4741 cagaatcccg gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt
     4801 aatgtaatca cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag
     4861 gcaaatggtg aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac
     4921 cacggcatta aagatgtgcg caccgtcaac atgcagcgcc agattgcgct cgcgggtaaa
     4981 ttcccatgct tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttccag
     5041 actgagtaat ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt
     5101 atccagcggt agcgtgccgt cggcagccgc gtcgatgggt tgcggctgaa tactgcccag
     5161 caccgctgcg ccaccggctt caaacagata gttatgcgcg gcctgaccga caatatactc
     5221 ttcgccacgt tcgcagtgac tgagcagagc gaccaggttg gcctgagtgc cggtaggcag
     5281 aaaaatggcg gcttctttac cggaaagctc tgctgcatag tcctgcagag cattaacggt
     5341 agggtcgtct ccgtaaacgt cgtccccaac cggggcggcc atcatcgctt cgagcatggc
     5401 gcggctcggt cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga
     5461 cgggaaatgc cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc
     5521 gccgcgtccg ctgataattg cgcgcaacat atacaggcta aaacctttgg cctgttcgag
     5581 tttgatctgc ggtggaatgg ctaattcttc tttggcgacc accacatcca ccaacaccgg
     5641 accgtcgata gagaaggcgc gttgcagggc ttcatcaact tcagaggctt tttctacacg
     5701 gatacccgta atgccgcacg cttcggcaat gcgggcaaag ttggtgtcgt gcagctcagt
     5761 accgtctgtc aggtaacctc cggccttcat ctccatcgcc acaaagccca gcacgctgtt
     5821 attaaagacg ataattttca ccggcagttt catctgcact actgagagga aatcgcccat
     5881 caacatgcta aaaccgccat cgccgcacat ggcgaccacc tgacgttccg gctcggttgc
     5941 ctgcgcaccc aacgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa
     6001 caggcgacgc ttgccgttca ttttcagata acgtgccgcc cataccgttg gcgtgccgac
     6061 gtcacaggta aagatggcgt catcggcggc aaaatgacta atttgctgcg ccagatattg
     6121 cgggtgaatg gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcatcgcg
     6181 gtaatcttcc agcgctttat ccagaaactt gcgatcggtt ttttcttcca ccagtggcag
     6241 caatgcgcgc agggttgatt tgatatcgcc gaccagcgcc atatctacct tgctatgcgc
     6301 gccgatgctg gctggattaa tatcaatctg aataattttg gcatcggtcg ggtagaacgc
     6361 acggtagggg aactgagtac cgagtagcac taacgtgtcg gcgttcatca tggtatggaa
     6421 acctgacgag aatccgatta acccggtcat tccaacatca tacggattat cgtattcgac
     6481 atgctcttta ccgcgtaggg catgaacaat aggtgcttta attttcccgg caaactcaac
     6541 taactcttta tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata
     6601 acgcagcagt tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggctgtgg
     6661 cgcatgatac cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcccgg
     6721 taacacaaca acggaaacgc cacggttaag caccgctttg cgcatagcaa ttgccagcac
     6781 ttgtgggatc tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag
     6841 ctcttgtggg tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc
     6901 ggcaatcgcc agtaccggaa cgttattgcg gtggcaatcg aataggccgt tgatcaagtg
     6961 caggttgcct gggccgcacg atccggcaca gaccgccagt tctcctgaaa gttgtgcttc
     7021 agcgccagcg gcaaacgccg ccacttcttc atggcgggtg gacatccact cgatggtgcc
     7081 catgcgatta agactgtcgc taagaccgtt cagagagtcg cccgtgactc cccagatgcg
     7141 tttcacgcct gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt
     7201 tctccatctc ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg
     7261 gatgggagag aaaggaggca ctaacggtta aatagcccga tgaaaggaat atcatcgggc
     7321 ataaggcgat tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta
     7381 accttcagcg atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga
     7441 aaccactttt gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac
     7501 gttattgctt tccagcgcct ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc
     7561 cggttgcagt ttggtgaatt tcagaccgct ggtcgccgct tctgctactg gggtgaagaa
     7621 tttctcttta aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat
     7681 atacggagcc gggccgcagg tcatcacggt acgtgaagct aagttaggta catctgccag
     7741 cagttcgcga gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag
     7801 cgttaccgga tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt
     7861 gtagatcacc cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat
     7921 aatcggcgtg acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc
     7981 gcaggtaaat tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg
     8041 cgtcagccac tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc
     8101 actcacgcct ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac
     8161 gctgaccagt gcatattgcc cggcgcgata tgggtagtga tcgtggcaaa tcagggaaat
     8221 cgtccacaca tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg
     8281 attcgttggc atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct
     8341 tcaacagtgg tgatagaacg caggccgaat ttctcgttca gcaccgccag caggtctggt
     8401 gtcaggaaac taggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc
     8461 gtcagcagaa tgacaatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga
     8521 tcgttcacac cacagcccag tttctctgcc agagtgacag ccagaataat tgctgagtaa
     8581 gcatcgttac attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca
     8641 agtttgttaa agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg
     8701 ctggtggcga aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc
     8761 aggaagatat gacgcagttt ttcacggcta accagatcaa tcagcgtatc ggctgcacca
     8821 agcagcgtct ggcgaccaaa gcccacggtg atcaggtgag gaatttcgct gtacgggaag
     8881 cccgccattt gttgcgcctg ggcgatgacc gcagagaaat cttcgccatc cagatgacgc
     8941 acgccaggcc agccaacgat gctgcgggtc cagatacgat cgtcataagc gcctacggtt
     9001 gggtcgatga tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact
     9061 tgctgattct gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc
     9121 gggtagccat gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg
     9181 gtctgttcca gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta
     9241 cccgccgtcg ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg
     9301 cctgcatcca gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca
     9361 agaagcgcgt tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg
     9421 gcataaatat cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca
     9481 cctttcaggc catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct
     9541 ttatctttgt taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcatcgctc
     9601 accagttgca ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat
     9661 tgtgctttca gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga
     9721 gaatcgaagt taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca
     9781 tcgtggttga tgatgccgta ttcacgcgct tttaccgccc aggcagaaag cccttgcagc
     9841 gccgcgatga gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc
     9901 gcgtatgagc agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac
     9961 atgatcacac cttttaaagt tatatttaat atacatgttt aaggttaaga tgcttaacgc
    10021 ggggataaaa gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg
    10081 gcagggatct accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta
    10141 acaggctaag aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac
    10201 gttgaagaac gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag
    10261 agcggctgcg gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat
    10321 cattaaaaaa cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg
    10381 aaagagaata ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa
    10441 gaataaaggc gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa
    10501 tcattccccg gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa
    10561 taccaacgag gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta
    10621 agaaagccag tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta
    10681 gcgactccag cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc
    10741 acggcaggcc tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa
    10801 tactgacggc agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg
    10861 ccagactgat acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa
    10921 taacttttaa cgcagcttgt tggcgaagcg gaatgaggta acccacaatc aggggaacca
    10981 gaatgattaa cagcccagaa aacatgaaaa tccagtcctt gcaaagaaga agtcgaaatg
    11041 cgcgatgaca cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt
    11101 atgcatcaga gggggatttt taatcctatc tgagtgttta gcattgttgc gctcctttca
    11161 tgtgcaatgc catataaagc cactggcgga gggagcgcgc agggggcagc cagtcgccgc
    11221 cgccccctgc acccccgggc tctggcgaac aaaatcgccg ctgcgcggta ccttcagctt
    11281 atccccttcg gcaaccaggt cgggcgcgag gtaacgtcct gtaagacgcg ccctcagccc
    11341 acatccatgt gggctgcccc ggccttcggg gaacgcttca gcgattttga cgccaccaat
    11401 acccgtgcgg tttgtaatgt cgaagataaa acaaccaggg cagataaaat gccaatgcta
    11461 attaatcacg cttttccagc agagtccggt aaatcagacc accgataatg ccgccgacaa
    11521 ttggcaccac ccagaagaac cacagttgtt ctaatgccca gccgccctgg aagatagcaa
    11581 ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac cggaatactg attaagtgaa
    11641 tcagggttaa ggccagacca atagcgatcg gcgcaaaacc tgccggagcg aatttgtcgg
    11701 ttgcaccgtg gatcactaac aggaagcccg cactcaatac cagttcaact accagcgcgg
    11761 aaagcatgga ataaccgcct ggtgaatgct cggcataacc gttagaagca aaaccgctgg
    11821 ctgccgcgtc aaaacccgtt ttaccactgg caattaaata cagcagcgcc gctgcaacaa
    11881 taccgccgac aacctgggca atcacgtagc caacgacttc ttttgccgga aaacgtccgc
    11941 cagcccataa accaatagtg accgccgggt taaaatgacc accagaaata tgaccaacag
    12001 cgaaggccat cgtcagaacg gtcaaaccaa atgccagcgc cacgccggca aaaccaatgc
    12061 ctaattccgg gaagcctgcg gccagtacag cactaccaca gccaccaaaa acaagccaga
    12121 aagtaccaaa acattcagct gctaattttc tgaacatatc cacctcaatt aaaaattgac
    12181 cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa aatgacgacg gaaataatgc
    12241 gcggctattt taaaaacgaa ggcgagtcat tcaccagata aataaatcca gtaaatttga
    12301 tttagggcaa cagcgggttg ccccatatag tcatttgtct gattgacagt gtagtgcaag
    12361 caaaagattt aatcctttag gcgtaataaa aaataattta tcatgctaat tatttgattt
    12421 tgttgttttt gcgggcttat cagcaagagg gagtataacg cgattattcg ctcatttttc
    12481 agacaattgc catgcttaaa tgtgatgtca tcacgtatta gcaaggcctt tcccgttata
    12541 ctgccagcgt aaaggataag tcacatattt ctggagggga tatgattctt gagcgcgttg
    12601 aaattgtggg ttttcgcggt atcaaccgtt tgtcgttgat gctcgaacaa aacaacgtcc
    12661 tgattgggga gaacgcgtgg ggtaaatcca gcttgctgga cgccttaacc ctgctgctat
    12721 cgccagaatc agatctctac cattttgagc gcgacgattt ctggttcccg ccgggagata
    12781 tcaacgggcg agaacatcat ctgcatatta ttttgacctt ccgcgaatcg ctgccaggcc
    12841 gacatcgggt tcgccgttat cggccgctgg aagcgtgctg gacgccatgc accgatggct
    12901 atcaccgtat tttttatcgt ctggaagggg agagtgcgga agacggcagc gtgatgacac
    12961 tgcgcagttt tctcgataaa gacggacatc cgattgatgt tgaggatatt aacgatcagg
    13021 cacgccatct ggtgcgttta atgccggtgc tgcgcttgcg tgatgcccgt tttatgcgcc
    13081 gtattcgtaa cggcacggtg ccaaatgtcc ccaatgtgga agtcaccgcg cgccagctcg
    13141 atttcctcgc ccgtgagttg tcctcacatc cgcaaaatct ctctgatgga cagattcgtc
    13201 agggactttc cgcaatggtg cagttgcttg agcattattt ctctgagcag ggggctggac
    13261 aggcgcgata tcgtttaatg cggcggcggg ccagcaatga gcaacgaagc tggcgctatc
    13321 tggacatcat caaccggatg attgaccgac ctggagggcg ctcgtatcgg gttattttgc
    13381 tcggcctgtt tgctacttta ttgcaggcaa aaggcacatt gcgactggat aaagacgcgc
    13441 gtccgttatt gctaattgaa gatccagaaa cccgcctgca tccgattatg ctttccgttg
    13501 cctggcatct gttgaatctt ttgcctttac agcgtattgc taccaccaac tctggcgagc
    13561 tgctttcgtt aactccagtg gaacatgttt gccgcctggt gcgtgaatct tctcgtgttg
    13621 ccgcctggcg tctggggcca agtggcttga gcaccgaaga cagccgccgc atctcttttc
    13681 acattcgttt taatcgtccg tcatcgctgt ttgctcgctg ctggttgctg gtggaagggg
    13741 aaacggaaac ctgggttatc aatgaactgg cgcgtcagtg cggacatcat tttgatgccg
    13801 aagggatcaa ggtcattgag tttgcccagt ccgggctaaa gccactggtt aaatttgccc
    13861 gccgaatggg gattgaatgg catgtactgg tcgatggcga tgaagcaggg aagaaatatg
    13921 ccgctacggt acgcagcctg ttgaataacg atcgggaagc cgaacgagaa catttaacgg
    13981 cgttaccggc gctggatatg gaacatttta tgtatcgcca gggattttcc gatgtgttcc
    14041 accgcgtggc gcaaatcccg gaaaatgtac cgatgaatct acgcaaaatt atctcgaaag
    14101 cgatccatcg ctcttccaaa cccgatcttg ccattgaagt ggcaatggag gcaggacgtc
    14161 gtggtgtgga ctccgtacca acgctgctga aaaaaatgtt ctcacgcgtg ctgtggctgg
    14221 cgcgcggtcg cgcggattaa ccgcgaaaca tcgtggccat ttgtggctga atagcgtcga
    14281 gcatctcata gcgccgacgg tattcagccc gttttttact ggcgatttcg gcaatctctt
    14341 ttcgtgctat ctgtgctgga aggcggtaat ggcgttcagc atcacatacg ccgccaaccg
    14401 actcccagaa cgcgttgtaa tcagcatgga tcttgccttc tttatcgcga taacgcaggc
    14461 tgcggtaaat atgcgtttca ttgctgacgg caataatctg ctctacctgc aaacgttggg
    14521 caaacagaca ggccgcttcc atcacgaggc gtttgggaaa tagcccgtgg caggctttcg
    14581 tcgcattctg gatttcctga tgtggaattt cccattttgc gccttgcagt ccgccaataa
    14641 acatcgttct tttcccctga tattcacaca gggtaaacgt gatctctgcc agaggaatac
    14701 cttcgctgtt gcggaacagg attgtgctgt caccttcttt atccattgag atcatcatgg
    14761 tcagctcaag cgtgaactgc tcaccgtttt tgccttccag tttagccagt tgcagcccgg
    14821 gggtattcaa atataagctg aattcttccg ccgacataca cccgcggagt aacgcataat
    14881 ggtaacgtaa cgcctccagc aattgcttac ggctaagatt cgccgcaagg taagggcgat
    14941 gcagacgcac aggcagtcgc ggctggcgcg ttaacaacac attgagatta ggccagtggg
    15001 aaagttcgtt catccactca acgcttaaac gcggcataat caacgagcgc agcaaaaatt
    15061 tctggcgaaa actacagcga tgccagaatt tacccggccg acactgtcca cgtgccagac
    15121 taagaaaaag tgacaggctg ctgagagatt cagatggcgt aaaggtccgt tcagttagct
    15181 gcgacatatt catgaaatca atggttatac atgacgtcga tttcaccatt gcgtatctta
    15241 accaaacatc aatagtgtga ttactaacgt aaattttagg attttgttga tatttcgttg
    15301 aagttaatga cccggattgg catgtggagt attcagaata tttatgaaaa agcggaaaac
    15361 cgtgaagaag cgttacgtta ttgcgctggt gatagtcatc gccggactga ttacgttatg
    15421 gagaattctt aacgcacccg tgccgactta tcagacactg attgtgcgcc ccggtgattt
    15481 acagcaaagc gtgctggcga ccggaaagct ggacgcgctg cgtaaggttg acgtaggcgc
    15541 gcaggtcagc ggtcagttga aaacgctgtc ggtggcgatt ggcgataaag taaaaaaaga
    15601 ccagctttta ggggtcattg atcctgaaca ggctgaaaac cagatcaagg aggtcgaagc
    15661 aacgctgatg gagctacgcg cgcagcggca gcaggcggaa gcggagctga aactggcgcg
    15721 ggtgacgtat tcccgtcagc aacgtctggc acaaacgcaa gcggtttcac tgcaggatct
    15781 cgataccgcc gcgacggaga tggctgtgaa acaggcgcaa attggcacca ttgacgcgca
    15841 aatcaagcgc aatcaggcct ctctcgatac ggctaaaacc aatctcgatt acacccgtat
    15901 cgttgccccg atgggcgggg aagtgacgca aatcaccact ctgcaaggcc agacggtgat
    15961 tgccgcacag caagcaccga acattctgac gctggcagat atgagcacta tgctggtaaa
    16021 agcgcaggtt tctgaagcgg atgtaatcca cctgaagccg gggcaaaaag cctggtttac
    16081 ggtgcttggc gatccactga cgcgctacga ggggcaaatc aaggatgtgc taccgacgcc
    16141 ggaaaaggta aacgacgcta ttttctatta cgcccgtttt gaagtaccca accccaatgg
    16201 tttgctgcgg ctggatatga ccgcgcaagt gcatattcag ctcaccgatg tgaaaaatgt
    16261 gttgacgatc cctctgtcgg cgttaggcga tccggttggc gataatcgtt ataaagtcaa
    16321 attgttgcgt aatggtgaaa cacgcgagcg tgaagtgacg attggcgcac gtaacgatac
    16381 cgatgttgag attgtcaaag ggctggaagc gggcgatgaa gtggtgattg gtgaggccaa
    16441 accaggagct gcacaatgac gcctttgctc gaattaaagg atattcgtcg cagctatcct
    16501 gccggtgatg agcaggttga ggtgctgaag ggcatcagcc tcaatattta tgcgggtgag
    16561 atggtcgcga ttgttggcgc ttcgggttcc ggtaaatcga ccctgatgaa tattctcggc
    16621 tgcctggata aggccaccag cggcacctat cgcgtagccg gtcaggatat tgccacgctg
    16681 gacgccgatg cgctggcgca actgcgccgc gagcatttcg gctttatttt ccagcgttac
    16741 catttgcttt cgcatttaac cgccgagcag aacgttgaag tacccgccgt ctatgccggt
    16801 cttgagcgga aacagcgact gcttcgtgcc caggagttgc tgcaacggct ggggctggaa
    16861 gaccgcacag agtattatcc ggcacagctt tcgggtggtc agcaacagcg cgtcagcatc
    16921 gcgcgggcat tgatgaacgg cggtcaggta attcttgccg atgaaccgac cggcgcactg
    16981 gacagccatt ctggcgaaga ggtgatggcg atcctgcatc agctgcgcga tcgcgggcat
    17041 acggtgatta tcgtcaccca cgatccgcag gtcgctgctc aggccgagcg ggtgatcgaa
    17101 attcgcgacg gcgaaattgt gcgcaatcct cccgccattg aaaaagtgaa tgttgctggc
    17161 gggacggagc ctgttgtcaa tacagtgtct ggctggcggc agtttgtcag cggttttaac
    17221 gaggcgctga cgatggcatg gcgggcgctg gcagcgaata aaatgcgtac tttactgacc
    17281 atgctgggga ttattatcgg tattgcgtcg gtggtttcca ttgtcgtggt gggtgacgcc
    17341 gccaaacaaa tggtgctggc ggatattcgt tctattggta cgaatactat tgatgtctat
    17401 cccgggaaag attttggcga tgacgatccg caatatcagc aggcgctgaa gtacgacgac
    17461 ttaatcgcca tccaaaaaca accgtgggtc gcctcagcca cacctgccgt ctcgcaaaac
    17521 ctgcgcctgc gttataacaa tgttgatgtt gctgccagtg ccaatggcgt gagcggcgat
    17581 tattttaatg tctatggcat gaccttcagt gaaggaaaca cctttaatca ggagcagctg
    17641 aacggtcgtg cgcaggtcgt ggttctcgac agtaatactc gccgccagct tttcccccat
    17701 aaagcagatg tggttggcga ggtgattctg gtcggcaata tgcccgccag agtcattggt
    17761 gtggcggaag aaaaacagtc gatgtttggt agcagtaaag tgctgcgtgt ctggctacct
    17821 tacagcacga tgtccgggcg agttatgggc cagtcgtggc ttaactccat tactgtcagg
    17881 gtgaaagaag gatttgacag cgccgaggcg gaacagcaac tcacgcgttt actttcactg
    17941 cgccacggta agaaggattt ctttacctgg aacatggacg gcgtcttgaa aactgttgaa
    18001 aagaccacac gtactttaca actgtttctg acgctggtgg cggtgatttc gctggtggtg
    18061 ggcggtattg gtgtaatgaa tattatgctg gtgtcagtga ccgagcggac gcgggaaatt
    18121 ggcattcgca tggctgtagg tgcgcgagca agcgatgttt tgcaacagtt cctgatcgaa
    18181 gccgtactgg tttgcctggt cggtggcgcg ttgggaataa cactgtcact gttaattgct
    18241 ttcaccttgc agcttttctt acccggctgg gagattggtt tttcaccgtt ggcgctgctg
    18301 ctggcgtttc tctgctcgac ggccaccggg attttatttg gctggttacc cgcacgaaat
    18361 gcggcacgac tggatccagt agatgctctg gcacgggagt aatttttgag ataaaaatgc
    18421 cagccgatcg ggctggcatt ttgcctttag gatgtacaca atgagacaga agagctatgc
    18481 gactgccgct tctacttcga cgggcacaat aacactggcg tgattgcctt ttggcccctg
    18541 gtggacatca aactgaacgg attgtccagc ttttagcgtt ctgtaaccat ccatctgaat
    18601 ggtggaataa tgagcgaaaa tatcttcgcc gccgccttca gggcagatga aaccaaaacc
    18661 tttggcattg ttgaaccact taacagtacc cttttccatg cttcgacatc cttcgcaaat
    18721 cttatacaag taagatggaa taaaccgggg tcagagaggg ggctgttcaa aacctcgcca
    18781 actctagaaa tacaatttag agaattaggg cgagccgtca agcatttgac aggggccagg
    18841 gggcaggtat gaatcaaaaa tttgaagcag ttaacgctat tgacaggaat gtgacagatg
    18901 tcgctgatgc caacgataga tgatagttat ctatcatgtg gagtagattg gtcaggcaaa
    18961 taagctcttg tcagcggcag ggcgttctgc cgataaccgt aaccgaagat gataactgac
    19021 aatgggtaaa acgaacgact ggctggactt tgatcaactg gcggaagaaa aagttcgcga
    19081 cgcgctaaaa ccgccatcta tgtataaagt gatattagtc aatgatgatt acactccgat
    19141 ggagtttgtt attgacgtgt tacaaaaatt cttttcttat gatgtagaac gtgcaacgca
    19201 attgatgctc gctgttcact accaggggaa ggccatttgc ggagtcttta ccgccgaggt
    19261 tgcagaaacc aaagtggcga tggtgaacaa gtacgcgagg gagaatgagc atccattgct
    19321 gtgtacgcta gaaaaagcct gaatgcaggc ataaaaattg ggggaggtgc ctatgctcaa
    19381 tcaagaactg gaactcagtt taaatatggc tttcgccaga gcgcgcgagc accgtcatga
    19441 gtttatgacc gtcgagcact tgttactggc gctgctcagt aacccatctg cccgggaggc
    19501 gctggaagcg tgttctgtgg atttggttgc gctccgtcag gaactggaag cctttattga
    19561 acaaaccaca cccgttctgc ctgccagtga agaggagcgc gacacacagc cgacgctgag
    19621 ttttcagcgt gtactgcaac gtgcggtctt ccatgtccag tcctccggtc gcaatgaggt
    19681 taccggtgca aacgttctgg tcgctatctt cagcgaacag gagtcgcagg cggcatatct
    19741 gttgcgtaaa catgaagtca gccgtctcga tgtggtgaac tttatctctc atggcacgcg
    19801 taaagacgag ccgacacagt cttctgatcc tggcagccag ccaaacagcg aagaacaagc
    19861 tggtggggag gaacgtatgg agaatttcac gacgaacctg aaccagcttg cgcgtgtggg
    19921 cggaatcgac ccactaattg gtcgtgagaa agagctggag cgtgctattc aggttctctg
    19981 ccgtcgccgt aaaaacaacc cgctgctggt gggggaatct ggtgtcggta aaaccgcgat
    20041 tgcagaaggt cttgcctggc gaattgttca gggtgatgtg ccggaagtga tggctgactg
    20101 tacgatttac tctctcgata tcggttctct gttagcgggc acaaaatatc gcggcgactt
    20161 tgaaaaacgt tttaaagcgt tgctcaagca gctggagcag gacactaaca gcatcctgtt
    20221 tattgatgag atccacacca ttatcggtgc gggtgcagcg tctggtggtc aggtcgatgc
    20281 ggctaaccta atcaaaccgt tgctctccag cggtaaaatt cgcgtaattg gttcgacaac
    20341 ctatcaggag ttcagcaaca ttttcgagaa agaccgtgct ctggcgcgtc gcttccagaa
    20401 aattgatatt actgaaccgt cgatcgaaga aactgttcaa atcatcaatg gcctgaaacc
    20461 gaagtatgaa gcgcaccacg acgtgcgtta taccgcaaaa gcggtgcgtg cggcggtaga
    20521 gctggcggtg aaatacatta acgatcgtca tctgccggat aaagccattg atgttatcga
    20581 cgaagcgggc gctcgcgcac gtctgatgcc ggtaagcaaa cgcaagaaaa ccgttaatgt
    20641 ggcggatatt gagtccgtgg tggcccgtat tgcacgcatt ccagagaaga gtgtttctca
    20701 gagtgaccgc gataccctga aaaacctcgg cgatcgcctg aaaatgctgg tcttcggcca
    20761 ggataaagcc attgaggcgc tgactgaagc cattaagatg gcgcgtgcag gtttaggtca
    20821 cgaacataaa ccggtcggtt cgttcctgtt tgccggtcct accggggtcg ggaaaacaga
    20881 ggtgacggta cagctttcga aagcgttggg cattgagctt ctgcgctttg atatgtccga
    20941 gtatatggaa cgccataccg tcagccgtct gattggtgcg cctccgggat acgttggttt
    21001 tgatcagggc ggtttgctga ccgatgcggt aatcaagcat ccacatgcgg tgctgctgct
    21061 ggacgaaatc gagaaagcgc atccggacgt gttcaatatt ctgttgcagg tgatggacaa
    21121 cggtacgctg accgataaca acggacgcaa agcggacttc cgtaacgtgg tgctggtgat
    21181 gaccactaac gccggggtac gggaaactga gcgcaaatcc attggtctta tccaccagga
    21241 taacagcacc gatgcgatgg aagagatcaa gaagatcttt acaccggaat tccgtaaccg
    21301 tctcgacaac attatctggt tcgatcatct gtcaactgac gtgatccatc aggtggtgga
    21361 taaattcatc gtcgagttgc aggttcagct ggatcagaaa ggtgtttctc tggaagtgag
    21421 ccaggaagcg cgtaactggc tggccgagaa aggttacgac cgggcaatgg gcgcacgtcc
    21481 gatggcgcgt gtcatccagg acaacctgaa aaaaccgctc gccaacgaac tgctgtttgg
    21541 ttcgctggtg gacggcggtc aggtgacggt tgcgctggat aaagagaaaa atgagctgac
    21601 ttacggattc cagagtgcac aaaagcacaa ggcggaagca gcgcattaat ctgattgtca
    21661 ggtaggttgg tgaagtccgt aatctcgtca ggggttacgg actttttatt tatggggtgg
    21721 aacggtgaaa accctatttt tggaggtgaa ggtaagttgt tgataattag tgctgctgga
    21781 aggtaaggat aaaaaagggt gctgcaggag aatgggatgg ttttgcttta ttaacaacgg
    21841 gctaaacgtg tagtatttga gttcactgcc gtacaggcag cttagaaatc gacggggtgc
    21901 ggtaaaacct ttgcgaacgc gttcactgcc gtacaggcag cttagaaatt cacaggtaac
    21961 atactccacc cgcccaccat gttcactgcc gtacagacag ataaaatgcg aaaaaaaagc
    22021 tcgcactttc gtacgagctc ttctttaaat
//
LOCUS       DADLZM010000058        21644 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLZM010000058 DADLZM010000000
VERSION     DADLZM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.058"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..157
                     /locus_tag="JNY73_002145"
     CDS             <1..157
                     /locus_tag="JNY73_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY8048157.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="JNY73_002146"
     CDS             227..1033
                     /locus_tag="JNY73_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8048158.1"
                     /translation="MQTWLNLQDKIIIVTGSASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="JNY73_002147"
     CDS             1043..1450
                     /locus_tag="JNY73_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY8048159.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="JNY73_002148"
     CDS             1450..1944
                     /locus_tag="JNY73_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY8048160.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="JNY73_002149"
     CDS             2010..2807
                     /locus_tag="JNY73_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY8048161.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="JNY73_002150"
     CDS             2818..3606
                     /locus_tag="JNY73_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY8048162.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /locus_tag="JNY73_002151"
     CDS             3658..4887
                     /locus_tag="JNY73_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8048163.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="JNY73_002152"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="JNY73_002152"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY8048164.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="JNY73_002153"
     CDS             complement(6049..7680)
                     /locus_tag="JNY73_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY8048165.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNY73_002154"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNY73_002154"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY8048166.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="JNY73_002155"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="JNY73_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY8048167.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /locus_tag="JNY73_002156"
                     /pseudo
     CDS             12924..15108
                     /locus_tag="JNY73_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNY73_002157"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNY73_002157"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY8048168.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNY73_002158"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNY73_002158"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY8048169.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNY73_002159"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNY73_002159"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY8048170.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNY73_002160"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNY73_002160"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY8048171.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21577
                     /locus_tag="JNY73_002161"
     CDS             21287..21577
                     /locus_tag="JNY73_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048172.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5339 a         5549 c         5469 g         5287 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcagcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccag atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttactg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcgtc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaatggaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ccttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac ttgctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagg cggttgggtt
    21481 ctataagaag gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc
    21541 gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc gcccctgccg tgcctgccca
    21601 agctggagaa gacgcgccag cgtcgcaccc ggcatttttt tcac
//
LOCUS       DADLZM010000059        21633 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLZM010000059 DADLZM010000000
VERSION     DADLZM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.059"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(102..992)
                     /locus_tag="JNY73_002162"
     CDS             complement(102..992)
                     /locus_tag="JNY73_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY8048173.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1022..2278)
                     /gene="wzx"
                     /locus_tag="JNY73_002163"
     CDS             complement(1022..2278)
                     /gene="wzx"
                     /locus_tag="JNY73_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY8048174.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(2294..2839)
                     /gene="rfbC"
                     /locus_tag="JNY73_002164"
     CDS             complement(2294..2839)
                     /gene="rfbC"
                     /locus_tag="JNY73_002164"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY8048175.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(2844..3722)
                     /gene="rfbA"
                     /locus_tag="JNY73_002165"
     CDS             complement(2844..3722)
                     /gene="rfbA"
                     /locus_tag="JNY73_002165"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY8048176.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(3781..4680)
                     /gene="rfbD"
                     /locus_tag="JNY73_002166"
     CDS             complement(3781..4680)
                     /gene="rfbD"
                     /locus_tag="JNY73_002166"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY8048177.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(4680..5765)
                     /gene="rfbB"
                     /locus_tag="JNY73_002167"
     CDS             complement(4680..5765)
                     /gene="rfbB"
                     /locus_tag="JNY73_002167"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY8048178.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(6138..7031)
                     /gene="galF"
                     /locus_tag="JNY73_002168"
     CDS             complement(6138..7031)
                     /gene="galF"
                     /locus_tag="JNY73_002168"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY8048179.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(7206..8600)
                     /gene="wcaM"
                     /locus_tag="JNY73_002169"
     CDS             complement(7206..8600)
                     /gene="wcaM"
                     /locus_tag="JNY73_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY8048180.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(8611..9831)
                     /gene="wcaL"
                     /locus_tag="JNY73_002170"
     CDS             complement(8611..9831)
                     /gene="wcaL"
                     /locus_tag="JNY73_002170"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY8048181.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(9828..11108)
                     /gene="wcaK"
                     /locus_tag="JNY73_002171"
     CDS             complement(9828..11108)
                     /gene="wcaK"
                     /locus_tag="JNY73_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY8048182.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(11288..12766)
                     /gene="wzxC"
                     /locus_tag="JNY73_002172"
     CDS             complement(11288..12766)
                     /gene="wzxC"
                     /locus_tag="JNY73_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY8048183.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(12768..14162)
                     /gene="wcaJ"
                     /locus_tag="JNY73_002173"
     CDS             complement(12768..14162)
                     /gene="wcaJ"
                     /locus_tag="JNY73_002173"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY8048184.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(14217..15587)
                     /gene="cpsG"
                     /locus_tag="JNY73_002174"
     CDS             complement(14217..15587)
                     /gene="cpsG"
                     /locus_tag="JNY73_002174"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY8048185.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(15692..17128)
                     /gene="cpsB"
                     /locus_tag="JNY73_002175"
     CDS             complement(15692..17128)
                     /gene="cpsB"
                     /locus_tag="JNY73_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY8048186.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17131..18354)
                     /gene="wcaI"
                     /locus_tag="JNY73_002176"
                     /pseudo
     CDS             complement(17131..18354)
                     /gene="wcaI"
                     /locus_tag="JNY73_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(18351..18830)
                     /locus_tag="JNY73_002177"
     CDS             complement(18351..18830)
                     /locus_tag="JNY73_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY8048187.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(18830..19798)
                     /gene="fcl"
                     /locus_tag="JNY73_002178"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(18830..19798)
                     /gene="fcl"
                     /locus_tag="JNY73_002178"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY8048188.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENSQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(19801..20922)
                     /gene="gmd"
                     /locus_tag="JNY73_002179"
     CDS             complement(19801..20922)
                     /gene="gmd"
                     /locus_tag="JNY73_002179"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY8048189.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(20948..21496)
                     /gene="wcaF"
                     /locus_tag="JNY73_002180"
     CDS             complement(20948..21496)
                     /gene="wcaF"
                     /locus_tag="JNY73_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY8048190.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(21512..>21633)
                     /locus_tag="JNY73_002181"
     CDS             complement(21512..>21633)
                     /locus_tag="JNY73_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY8048191.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         5381 a         5709 c         4997 g         5546 t
ORIGIN      
        1 tgtctacatc tttttgttca ataatacttt ttaattgttc ctcaatccag cattcgccat
       61 tatacgtagc cataagtaca gctactcttt tttgatcaga actatgcatt ataacgaccg
      121 cccccagtaa ttcctcttat tccatccagc gtagccaaaa tatgtttttt catatattca
      181 attttatttt tttgagttgc gataattaaa gtcgtatgaa ttaaagaaaa aacaacttcc
      241 ctaatagaga gtaaaagagg cacatgattt tttctaagca aaagaaatgc atttcttact
      301 cgataataac gacgtactgg cgagtgaatt ggtatgttaa tcccacagat ttttactgac
      361 ttatccccaa tagcatgaac catttctata tcaggaatga tatgaactaa tatgccatag
      421 tttaagcatc taagacacca ttctgtatcg acataatcaa taaaaagttc ctcatccatc
      481 attccaacaa tctcaaaaac ttcttttgaa accatggttc cggatgagat tgttactgaa
      541 cttttaaacg gtgtctgtcc ttcagtgaca tgaattttct ctcgaagacc attttttttg
      601 atattacaaa taggataata atgtgactta tctctatcaa taaaaactgg gccagctatt
      661 tttgcatttt cattatccat atagcaggaa agttttttga taaactcatt gcgaatacgg
      721 ctatcttgat caaaaaaaat tatcttctct gccccttttt caagagaata tcgaatccca
      781 atattttgtg cttttgctat accttcattc ctgccaagac aaattaaatg aactttttca
      841 atgtctgcta atttatcttt caaggtgaaa ttagtcctat tatcgactac tacaacgtaa
      901 caattttgct cctttagttc agtgatcagt gcattcagat ttttaagttc gggattatat
      961 gtcacaatga cagcgtaaat attactattc attatccaag tgactcagta attggttaat
     1021 tttagttttc atatacagaa cacattctag tataaagcca ttgtaataac agagcatcta
     1081 aaattacacg tatgctccag gatattgcgg cacccagtaa cccatatttt tcgaccataa
     1141 aatacaaaag ggctaaataa ggaaccaatt cagcacaatg aatcaaagca gtaattttag
     1201 attttcctaa tgcctggatt gcagagaatg gtatcattgc cagcgcattg aagaaaaaac
     1261 cgatcaataa cacatttaat atattaaagg aattaatttg gtaattctca ccaaaccata
     1321 ttttcaatac aagccctgaa tataacaagc ctatgattat caccggcaaa cagattagaa
     1381 acattaaaag caatgattta tttacattac gtttaaattc tttaaagtct ttgatgttac
     1441 ttaaccttgg aaacactgcc ctcccgattg ctgcaggtat tattcctaat tttagaataa
     1501 cctccgctgg tgcagaataa aatgcaactt tatctgcccc cataatattt gacactataa
     1561 atcgatcaaa atacaccatg accggactta tgatattact tactgtcatc caaccgccaa
     1621 agaaaaagag tcgtttaaag gttttataac aaaaatgaac ccccgaaatt ttaatatcat
     1681 tcctgacata ataggcagag actaaaatcg aaataactct tgcaaatatt aaaccagcaa
     1741 ccgctgccga caatgtagca ctataaaaaa caaatatggc cggaattccc gcgatgcaag
     1801 agcttgatat agatttttga atatttacaa tgccaaattt ttcatccccc tcaagaatgg
     1861 ctgaccataa ttgatttaga ataaataatg gaatgcaaat agccaacagt ttaaatgcta
     1921 gttgtatatc actatgttca acaccagaaa tttttagata attaacaatt ccatcagaga
     1981 aaatcaataa taaaaaagca ccaaagcatg aaaatagcac caaaaaagat gtacttgttg
     2041 aaattacctt ggttctttca tgatgattat cacgatgaat tgcaatttct ctaataacag
     2101 agcgagtcag gcctacatca aaaattccag catacccaac aagtgcaatt gcaattgtat
     2161 aaacaccgaa accttcaggc cccaaacctc gagcgagaaa tcctagcgag gggatagcaa
     2221 ttaaagttgg tattgcatag ccaaaaaggt tccagacact attttttatt atgctcatta
     2281 ttacatttct ttatcatata aaataatcag catctacaaa tttttgacca ttaatatctt
     2341 tatcagaaag caatggattt tgaaccggcc attctatatt tatacttttg tcgctccata
     2401 aaatcccacg ctcttgcatt tttgaataga aattatttgt tttgtagaca aattctgctt
     2461 catcactaag aacgcaaaac ccatgagcga atccttccgg tatccataat tgacgcttat
     2521 tctccgccga caaatttaca ccaacccact tgccaaatgt tggtgatgat ttccgaatat
     2581 caacagctac gtcaaaaact tcaccgacaa cacagcgtac cagttttcct tgagcataag
     2641 gttccagctg atagtgcagt ccgcgtaaca cacccttagt tgatttagaa tggttatcct
     2701 gaacaaactc aacctttcgt cctacagcct cttcgaaaac tttctgattg aagctttcca
     2761 taaaaaagcc ccgctcatca ccaaatactt ttggttcaaa aattagcaca tcaggaattt
     2821 cagttttaat tacgttcatt ttattaataa cctttaatca ttttcagcag atactgacca
     2881 taagcatttt tcttcagcgg ctcggctaat accttcactt gctcagcatc aataaagcct
     2941 ttacggtaag caatttcttc cgggcaggaa actttcagcc cctgacgctc ttcaattgtt
     3001 gcaatgaagt tgcttgcttc aataagactt tgatgtgtcc ccgtgtccag ccatgcataa
     3061 ccacgcccca tcatagcgac agacaaacgc ccttggtcca tataaatacg gttaatatcg
     3121 gtaatttcca gttcgccacg tgcagatggt ttaaggtttt tcgccatttc tacaacgtca
     3181 ttgtcataga aataaagccc agtaaccgca tagttacttt ttggttccag cggtttttct
     3241 tccagactaa ttgccgtacc gttatcatca aattccacca caccgtagcg ctcaggatca
     3301 tttacgtgat aggcaaaaac cgttgcgcca ctctctctgt taacagcggt atccattaat
     3361 ttcggcaggt cgtgaccata gaagatatta tcaccaagta ccagcgcaca atcatcacca
     3421 cctataaatt cttcaccaat gataaatgct tgcgccagac catccggact cggttgtact
     3481 ttatattgca gattaagccc ccactggctt ccatcaccaa gcaattgttg aaaacgtggt
     3541 gtatcctgcg gcgtactgat aatcagaata tctcgaatac ccgccaacat aagcgttgaa
     3601 agcggataat aaatcatcgg tttatcataa atcggtagca gttgtttgct cacagccatt
     3661 gtcacaggat aaagacgagt gccggaacca ccagccagaa taataccttt acgcgttttc
     3721 atttcactat tccttttaat tcatctcgct ctggcatcat gagcgagatg caataatttg
     3781 ttaaattgcc gtggtcgtaa ataattcgtt gagcatacgt ttcacgccca cctgccagtc
     3841 aggcaagaca agcgcaaagt tctgctgaaa tttttcggta ttgaggcgag aattatgggg
     3901 acgacgtgct ggtgtgggat aggccgttgt cggcacggca ttaagtttgt taagagcaag
     3961 gttgatcccc gctctgcgcg cttcttcaaa taccagagcg gcataatcgt gccaggttgt
     4021 tgtaccacca gcaaccagat ggtacaaacc agcaacttct ggtttatttg cggccacgcg
     4081 aatggcatga gcggtgcaat cagccagtaa ttcagccccc gttggtgcgc cgaattgatc
     4141 gtttattaca gccaactctt cgcgttcttt tgccaggcgc aacatcgttt tggcaaagtt
     4201 atttccttta ccagcgtata cccagctggt acggaaaata agatgctttg cgcaatgttc
     4261 ttgtaacgct ttttccccag ctagcttggt ttcaccgtag acatttagcg gtgcggttgc
     4321 atccgtttcc agccatggcg tgtcaccgtt gcctgggaaa acataatcag tggagtagtg
     4381 tataacccag gctccgactt cattagctgc ttttgcaatc gcttcgacgc atgtcgcgtt
     4441 aagtaattgc gcgaaattcg gttctgattc cgctttgtct actgcagtgt gagcagcagc
     4501 attaacaata acatcggggc gaattttttt gacggtttca gccacacctt cggggttgct
     4561 gaaatctcca caataatcag tggagtgaac atcgagcgca atcaagttac ccagaggagc
     4621 cagagcacgc tgcagttccc aacccacctg ccctgttttg ccgaaaagca ggatattcat
     4681 tactgacggc cctcatagtt ttgttcaatc cacgactgat aagcaccgct tttcacattc
     4741 tcaacccaat ttgtattagc cagataccat tccaccgttt tacgaatccc gctctcaaac
     4801 gtttcttgcg gtttccagcc caattcgcgg ctaattttat cggcatcaat ggcgtaacgg
     4861 cggtcatgcc ccgggcgatc ggcaacataa gtaatttgct cacgataaga tttctctttc
     4921 ggtacaatct catccaacaa atcacaaata gtgagcacta cgtcgatgtt tttcttttcg
     4981 ttatgtccac caatgttata agtttcaccc gctttacctt cggttacgac ggtatataac
     5041 gcacgagcat gatcctctac atacaaccag tcgcgaattt gatccccttt accataaata
     5101 ggtaatgcct taccttccag agcattcaga ataaccaatg gaataagttt ttccgggaag
     5161 tgataaggac cataattgtt cgagcaatta gtcacaatgg ttggtaaacc ataagtccgc
     5221 ttccaggcac gtaccaaatg atcgctggaa gctttagacg cagaatacgg gctacttggc
     5281 gcgtaagctg tcgtttccgt aaatagcggt aacgcttcgt tattatttgc ttcatccgga
     5341 tggggtaagt caccataaac ttcatcagta gaaatatgat gaaagcggaa actttttttc
     5401 ttttcatcat tcagagcaga ccagtaattg cgcgccgctt ctaaaagaac ataagtcccc
     5461 acaatattgg tttcaataaa tgccgcaggg cccgttattg aacggtcaac gtggctctct
     5521 gccgccaggt gcatcaccgc gtctggctgg tgctgtgcga aaatacgcga catggctaca
     5581 gcatcgcaga tatctgcatg ctcaaaagca taacgttcag aatcagaaac atcagcgagt
     5641 gattctaggt tcccggcgta tgttaattta tcgacattaa caacactatc ttgcgtatta
     5701 tttattatgt gacgaacaac agcagaacca ataaaccctg cgccaccagt aacaagtatc
     5761 ttcacttttt tattccatat agctatagag catgctgtga aatagactgc tccagatttg
     5821 attaatagag gtattaatac acgctaccgc ccctggctta acagctacca gtgcactgca
     5881 tgcatctcaa cgatgtggcg agcgttagcc actcgcgcca aatccgaaaa attcaaacgt
     5941 taattgtctt accaaacagc cgtagaaaca aggaaaatcc tggaaaaatt tgaataatat
     6001 gagctatgta acttcttgtt tttataggct ttataaaaac ggcaacggca ggaaaattca
     6061 atgagcaaaa cttatgccac gaatcttcac tgccgttata attttcttat caaccgttac
     6121 atccggtcag attttcatta ttcgcttaac agcttctcaa tacctttacg gaacttcgcc
     6181 ccttctttca ggttgcgtag gccatacttc acaaacgcct gcatatagcc catttttttg
     6241 ccgcagtcgt aactgtcgcc ggtcatcagc attgcatcaa cggattgttt tttcgccagc
     6301 tcggcaatag catcagtcag ctgaatacgt ccccatgcac caggctgagt acgttccagt
     6361 tccggccaaa tatcggcaga aagcacatag cgacctacgg ccatgatgtc tgagtccagc
     6421 gtctgcggct gatccggttt ttcgataaat tcaacaatac ggctgacttt accctcacgg
     6481 tccagcggct ctttagtctg gatgacggag tattcagaga ggtcacccgg catacgtttt
     6541 gccagcacct ggctgcggcc cgtttcgttg aaacgtgcaa tcatggcagc aaggttgtaa
     6601 cgtagcgggt cggcgctggc atcgtcgatc acaacgtctg gcagtaccac gacaaatggg
     6661 ttgtcaccaa tggcaggtcg cgcacacaaa atggagtggc ctaaacctaa aggttcgccc
     6721 tgacgcacgt tcataatggt cacgcccggc ggacagatgg actgtacttc cgccagcagt
     6781 tgacgcttca cgcgctgctc aaggagtgac tctaactcat aagaggtgtc gaagtggttt
     6841 tcgaccgcgt tcttggacgc gtgagttacc aggaggattt ctttgatccc tgcagccaca
     6901 atctcgtcaa caatgtactg aatcattggc ttgtcgacga ttggtagcat ctctttgggt
     6961 atcgccttag tggcaggcaa catatgcatc ccgagacccg ctacaggaat aactgctttt
     7021 aaattcgtca ttatttcatc cacctgtaaa atggttgctg aattatagct tgttcgattt
     7081 ttttcgccag catcaattac cctgaattga ttactgaatt acttgtgatg ttacgccgct
     7141 tcgttgtgga ttgcagtagc attgttccta agtatggctc catttttcca ggaatggtcg
     7201 caaatctact ccctccgttc cggcaatcta aagttaatct tttccacatt aacaatatgg
     7261 tgattaatcc tgtcgatatc gacggagctt tgccctcttt cattcaccgc atgaacattt
     7321 gcaagagaca gcagtgtttc ttttttcgcc ataaagacgc cgcgaacgtc tttgcgcatg
     7381 tcgaagttca tgctcaatgc tggtccaact gaggattcct gcatcacatt gatatttcgt
     7441 ataaaaagat gttgcggctt gttgtgtaac tccagcgagg cacgcttcat ctcaatgtta
     7501 gttagcgaca caaaggagac ggcattcccg gcggagattt ggatgccgcg caatttataa
     7561 gcaagatggg tattatccag ttgaatatta ttcactcgga aattttgcgg tatcgagaga
     7621 tatttgcctt taattacccc atagccgatt aacatcccgg cgctattaat catttcaata
     7681 ttatcaatca cgaaattgtc acaaccgtaa atagcgactg tcgcgttatc aatgcccgct
     7741 ttcttactga aatccggcgt gatattgcgg gcattgatat tacgaataac aaaatgttta
     7801 ccattttcaa catggttcaa ctgccgacaa tccgatcccg tgatattcgc cacgacaaag
     7861 tttttcactg cctggtcttc cgggtagttg ttatcataag tgcttcccgc aaggcctatg
     7921 ccgatgcccc agttgatttt gccgttggta cagttgatac gctcgatgac atggtcagat
     7981 atcaaaatat cactgtcgtt aattgccacg ttccattcaa tggcgtcgcc ctgtaagtcg
     8041 ctgaacttac agttggtaat gttggcaccg ataatctggt tatgaaatcc ctggcgtaag
     8101 atggcgtaat tagcgtggct gacagtgagg ttatcgatgg tcaggttgcg catgacccgt
     8161 ttgtttttac cgccaatata aatctgcatt accgggccaa agccgctcat cgccagccct
     8221 ttgatggtgc agtcagaacc acgcacatcc agggtaatgt tatgcatacc gccgccctct
     8281 ccccctgtca cctggctgcc gtcctgtaag acaaatcgcc ctctgccgtt gccgcgcagg
     8341 cttccaagga tgtgtaacgt tttgccggga gggatgaaga tgccggtgtt gatattgtca
     8401 caaacgaatc cggcaggcac gacgaccgtt tgcccttcgc tgaaggcttg tttaaatgag
     8461 gcgatccagt catgtgggtt gtagtcgtta atgttaacgc tttgtcgggc gggaagtgcg
     8521 cgagcgaaag gggtatggag gaaggcaagc gccgagcttg ccgtcaggaa ggtgcgtcgg
     8581 gagagtgttt taaatggcat acgttctcct ctataaagcc tgcagcaagc tggcgagttc
     8641 tcgattaatc acctgctggt taaaatcgtg ttcgactttt tcgcgcgcac gtttgacgac
     8701 cggagccagt tcgtcggtat ccagttggct aaacgccgcc agacgttgtg ccagtgcgcg
     8761 agcatcgttc tcaggcacca gccagccgga tttatcggcc tccaccagtt caggaattcc
     8821 gctatgcaga gtagaaacca ccggaatacc gaccgccatc gcttccatta gcgccaccgg
     8881 aatgccttcc atatcgccat ccgcgcccgt taccgatggc aacaggaaga catccgcgtc
     8941 gtcgagcatc tccttcactt catggctcgg tttaaagccc ggcatctcta ccacatcttc
     9001 cagttgatat tgttcgatga gggtgcgcag gcgtcgttcc cacaggccaa tgccgaggat
     9061 gcgatagcga aatgccacgc cctgctcttt caactgacgg caggcttcaa tcgccacatg
     9121 caggcctttt ttctcggtta agcgtgcgac agatatgatt tccagcggcg ttgcgggcgc
     9181 tttcaccgga cgcgggctaa agcgcgtcat gtccacgccc atacgcgata cggcgatttt
     9241 ttctctgggg cagcccattt tttgcagcct tccggcccac agatcgctta tcggtaacat
     9301 caggtcgcca cggcgaaaca gttgttgata ttcgggagtg tagtggttga gcacttcccg
     9361 actggagata tcaataccgt ggaagatggt ggcaattttg ccgcgaatga tacccagttc
     9421 gcgtagtttt gctgcggtta cccccgccgg gccaaaatga gcgatgaaca catcggcgcg
     9481 aaacggtgtt gcgacctgac cacaaatggc agacaaaatc aggttccgcg actcggcacc
     9541 atagcgtttg aggttgagcg catgccaggt atttttacga tgaatgccac gtaaggtctg
     9601 gctggcgcgg tggcgcagtt tcgctacttt gcccgcaggt tcgtcctgta accagcgggt
     9661 tctggcgaca aggttgtatt tcgtccatgc cgcgtgggtg ttctctgtat cgcctttttg
     9721 caaagcgaca atctccactt caaagcccat atcaataaac gcggtaattt gattgaggac
     9781 gaaggtttcc gacgacagcg ggaatttcaa taagaagaag ccgaccttca tttcacctcc
     9841 ccgatgcgct caagcacaga ctgcaccatc tgcattcccg tctgacgttc gcgactgacg
     9901 gcttcgttaa atcgagcgtt cagcaccgga agctggccta aggtatccgc aaccatcgct
     9961 tgcaggctgc cgtccagtaa atgacggata tcaatcgcca tctctggcag tcccagctgc
    10021 tgcataatcc cggcggattt atgttcatag ttgatggcaa tcgccggagt agcaaagttc
    10081 attgagataa tggcggagtg caggcgcgta ccgacggtga gttcacaggc tccgagaatt
    10141 ttgcccattt ccagatcgtt gagttcatcc atcactacgt ggtaacgggc aggatcgctg
    10201 atgtgctggc gcagattgag cgccaccatg cggtcgtctt tgttatagct gtcgataccc
    10261 gtacaggtgg agagcgcaat cacctgatag ccttcatcga gaatgcgatt gaccaccccg
    10321 gcaaaggctt tttcatacgc ttgttgagtg gtgccgagac gtttgtcgaa cggtgccagt
    10381 tcgcgcaggg taattgccac tgttttctgt tgtgcggcaa cgtccagcca gtgttgaacg
    10441 gcatagctgg cggtgaagtc ttcggtgtgg tgatcgacca gccacgcggt atcgacgcca
    10501 tgttccactt ttgcggtggt gatattgctg cgtttcatca aatcaaggct gaccgattcg
    10561 cgcaggatca gcgcgtcgca gtgaccaaag acatagttcg ccagttggtt aaattgctca
    10621 tcctggaagg ggccgacgct gtgaccaatc ataaacagcg gctttttcgc cataaatgtg
    10681 caaagcgcat gttcaaactg cggcacgccg tagagatcga caaaaaacga tccgccgacc
    10741 tgaataatgg cgtcgtaacc tgacagcagg cgcacgaagt cggtgaatcc ctgggcgatg
    10801 gcgatattgc gcagcttgcc agtgtcggtg acgcgtgaga gcaatacctg atgctggtag
    10861 cggcgacgga ggactttttt aacgcgcccg acaacgcccg ccgcgctatt gtgttgtgtc
    10921 atctgcagga acagcggatc gcccattacc gggcggttga gcagccagga agaactgacc
    10981 ggatagcggc tcatcacgtc cacttcggta tgtggattga ggatgttgat ggcatcaagt
    11041 aagccgcgca agatggcgct gtcgccacga ttgccgcaag tgtggttgcc cagaataagt
    11101 aatttcattt tttcctcata aatttgatgc caggtgaggc tgtgtttgtt gtgttcgttg
    11161 ccgttttttg cctgatgcga cgctggcgcg tcttatcagg cctacaggtc ccgagcacag
    11221 aaccgtagga cggataaggc gttttacgcc gcatccggca accgttgtcg gaaccgaaaa
    11281 cagcaattca ccccgcccgt aaaagcattt tcattttttc gctgcgacaa aactgacgct
    11341 tcacttccac caccagcgga tggcgtgaca gcacaatcat caccacaaac gccagcacgc
    11401 ccgcggctat ttgcaccgcc agcagcatcc ccagcgccag ttgccctttc agcacaatgc
    11461 ccagcgcata actgaccacc agcgtcggca acgagagata aaacggcagc cacaaactca
    11521 ggatgtactg gcgataactg gaaccaagaa ccggtttaat catcacgaag taactcagaa
    11581 tggtgttgat aatctgcacc agcaggaaac cgagcgtgac gccgattgcg cccgccatct
    11641 gcccaccaat aacaatcgcc ggaataaaca gaaatgtttt gaatacattg aatttaaagc
    11701 tgatatcgac ccgcgctttc gccatcagca gcgaaccaat cgggttaccc acggagcgca
    11761 gcagacccac aatacacagc aattgcagca ccggaataat gctgttccac ttttcgccga
    11821 ataccagcgg tacgaagtta ttcgacacca ccatcagccc cagcagcgcc ggaaagttga
    11881 taatccccac tatcgacagc agcttgtaga agttaacacg cagcttttcg gtgtcgtcct
    11941 gaattttggc aaacgccgga aacaacacgc gggtgatgat tgggttcagc ttcatcggcg
    12001 gtacgacggc cacgttgtac gccaggttgt atccccctgc cacgcccgcg ccaagaatac
    12061 gcgccagcac cagcgttgaa aggttggtgt tgagatagtt gatgatgctg tccgccgtca
    12121 gccaggcacc aaaacgtaag ttcggtgcca cagacgccag cgagaagtgc agaccggggc
    12181 aataaatttt gcgtccaaag tagccaaaca gcagtgttct caccgcacta ttgaccagat
    12241 aaccgaggat cgcggtcatc gccagcggcc agaaatgggc gctaactacc gtaaaagtga
    12301 agcccgccag caccgcgctg gtttcgatca tgccgatttt gttgaactcc agctccttct
    12361 gcatcaacgc gcggaactgt tgcccgtggg ggatcaccac aaacgccagc gacaatgttt
    12421 taatcaacgg tgccaggtcc gggttattca gcacgtcgcc gatgacatca ctcaacaaaa
    12481 acaccgccac acataccaca agccccagcc cgacgttcag ccagtacagc gtggtgagtt
    12541 caaggtgact gatttctttt cgctgaataa tcgagttagc gataccgaag tcagaaagcg
    12601 tatctgccag cgcgataatc accagcgaca cagtcagcag gccgaactgg tggttgtcga
    12661 taatccgcgc cagcacggtc atctgcacca gcccgaggcc gatgatgatc accgtggcaa
    12721 tagccgacca cttcgcgccg ctgatggttt tttcacgtaa gctcatatca atatgccgct
    12781 ttgttaacga agcccttgaa taccgtcagg aaaacgattt tgatatcgaa ccagacgctc
    12841 cattcgcgga tgtactcaag gtcgaactcg acgcgttttt ccattttctc cagcgtgtcg
    12901 gtttcgccgc gccagccatt aatctgcgcc cagccggtaa tgcccggttt cactttatgg
    12961 cgcagcatgt agccttcaat gagctggcga tactgttcgt tatgcgctac tgcgtgcgga
    13021 cgtggaccga caatcgacat ccccccggtc agcacattga taaactgcgg caattcgtcc
    13081 agcgaggtgc ggcgcagaaa gttccccact ttagtgacgc gcggatcgtt ctgcgtcgcc
    13141 tgggtcacca ctttgtcgtt ctccatcact ttcatggaac ggaacttcca cactttgatc
    13201 ggcttgccat ccataccata gcgagtctgg cggaaaataa ccggccctgg tgaactgagt
    13261 ttcaccgcca gcgcaataca gcacagcacc ggggagatca gcagcagaat aagcgtcgcc
    13321 agcacaatgt cttccgcacg ttttagcagg cggttacccc ccgaaagcgg cgtgtcatac
    13381 agcggcacca ccggaacgcc gttcatctct tcgagacgtg aatggagaat gttgaaggta
    13441 aagacgtcgg ggatcagcag caccgaacag gtggtatccg ccagttgatg ggccagtttt
    13501 ttcactcgtg cgccgtcgca catttgcatc gcgatataga cgttatgaat cttgcccgct
    13561 tttgcatcct cgaccagctg ttgcaggtta cccgcccagt cgttagaaac gccgcccggt
    13621 ttcgggtcgt ggtaaacgcc caccacttca aaccctaacc acggctgatt acggaaactc
    13681 tccatcagca tttgtccggc ggctaaatcc cccgctacgg cgaccatccg cttgttgtag
    13741 ccatgattac gcagccagcc cgccccaatg cgaatacatg aacggcaaac caccagtccg
    13801 atgctggtca gcccatacca cgccagccag attttcagtt gcgtgtcgaa atcattgttg
    13861 aacgccacca gtccggcgct gaaaatcacg cttaaggtcc agttttgtag caacagggca
    13921 aattctgtcg ctgcccgaac accgcgccat gagcgataaa aatcggtgat gccgcccagc
    13981 atctggaaca ccaccagcgt aatcagcgcc accaacaggt gcatgtagag gaatgacagt
    14041 ccgctgactt cgcaaaccag ccatagcccg gcaaacatga tggtgatatc tgaaaagcgt
    14101 tgcaccatag agattaacga tgcattggtt ttcgctcgct cgcgcttttt tagatttgtc
    14161 atcgttgttc ctgttattag ccccttaccc gcagtggggt aagggaagat ccgacattac
    14221 tcgttcagca acgtcagcag agttcgcgtt cgcgcttcca tcagcggcac atctccgcgc
    14281 gattccacat tcaggcgcac caccggttcg gtattggagg tgcgcaggtt aaagcgccag
    14341 tcggcaaagg tcatgctgat gccatcgctg cgatccaccg ccagcgcctc acggctaaaa
    14401 tgctgttcca cgcggttaat cgcctcaacg ggttgcgcca gtttgctgtt gatctcaccg
    14461 cttgccggaa acgccgccat ccggtcgcgc accagttcgc ccagcgtttt tcctttcagg
    14521 cacaccagtt cggcgaccag cagccacggg atcatgccgc tgtcgcagta agcgaaatca
    14581 cggaagtaat ggtgggcgct catttcgcca ccgtagatgg cgtcttcctt gcgcatccgt
    14641 tctttaataa aggcgtgtcc ggttttcgac attaccggcg tgccgcctgc agcagtcacc
    14701 acatcaatag tgttccagga gagacgcgga tcatggatga tcttcgcgcc gggatttttt
    14761 tcgaggaacg cttctgccag caggccgaca atgtagtagc cctcgataaa ctgccctttt
    14821 tcgtcaaaca gaaaacagcg gtcaaaatcg ccatcaaagg caatgcccat atccgcgccg
    14881 tgtttgatga ccgcattgcg ggtgtcgtcg cgacattccg gcagcagcgg gttaggaata
    14941 ccgttgggga aattgccgtc cggtgtgttg tgtactttga ttaattccac gggcgcgccg
    15001 agggctttaa agcgggcttc aatggcgtcc accaccggac ccgctgcgcc attcccggag
    15061 ttgatcacca gcttgagcgg cgtgagattc ttgacattga tataaccgaa caggtgatca
    15121 acgtaagcgt cacgcaggtt gatttgctga tagcgaccgc gtttggtttc atcgacggga
    15181 ggaaagtcgt tggcttcagc cagacgctgg acgtcgcgca gtccggtatc accgctgatc
    15241 gggcgagccc cctcgcgcac cagcttcatg ccgttgtaat ccatcggatt atggctggca
    15301 gtaacttcaa tgccgccatc cacgccgaga tggaacgtgg cgaaatagat ctcttcggtg
    15361 ccggacatac cgatatccag cacatcgacg cccgcatcct gtaaaccttt tgccagcgcc
    15421 agttttaagg tttcgctggt gagacggaca tcaccgccta acacaatggt tttcggtttg
    15481 agaaattcgc cataggcgcg accaatgcgc caggcgatat cttcattcag ttcttcgcct
    15541 aatttcccgc gaatatcata ggctttaaag caggttaatt ttttcatgtc gttacccttt
    15601 ttcaggcaat agttggccct gaccgaaacg gccaatttta tttttgtcgt tatttacacc
    15661 gcggtttcgc attcattgcc tgatgcgacg tttacacccg tccgtagcga tccgcgaagc
    15721 gcaccacatc atcctcttcg agataagagc cggagcgcac ttcaattaaa tcgagcggaa
    15781 ttttccccgg gttttccagg cagtgcgtcg cccccagcgg aatataaatg gactcgtttt
    15841 caccaagcag tttgatatca ccatcaatgg tgacttttgc cgttcccgcg accactaccc
    15901 agtgttccgc gcggtgatgg tgcatctgta ccgacaaacc ctcgcccggt ttcacggtga
    15961 tgcgtttcac ctggtagcgg tcgcccgcgt cgatagagtc atatttgccc cacggacgat
    16021 acacttcgcg atgcacccga tgctcatggc gaccatcggc tttgatctgc tcgaccactt
    16081 ttttcacatc ctgtaccgcg ttacggtcgg caatcagcac cgcatctttg gtctgcacca
    16141 ctaccagatc tttcaccccg acggtggtga ctaggcctga ttcggcatac acatagctgt
    16201 tttcagtttt gtgattaatc acatcgccgt ggcaaacgtt gccctcggcg gtgtgggcgc
    16261 tgatctccca taatgaagac caggaaccaa catcgctcca gcccgcatcc atcggcacca
    16321 caacagcatc tgccgtacgt tccatgaccg cgtaatccac cgactcttcc gggcaggcga
    16381 gaaacgcttc ttcatctaca cgaataaaat cgagatccgg atcgacggcg ctcatcgctt
    16441 tttcgcaggc atcgaggata tccgggcgat attttttcag ttcttcgaga tagcgtccgg
    16501 cgcggaacag gaacatgccg ctgttccagt aatattcgcc gcttgctaca taggcctggg
    16561 cggtttccag attcggtttt tcgacaaact gcgccacttc aaaggccacc gtatcctgct
    16621 cacccgcaga cacttcaccg cgacgaatat agccataacc ggtttctggt agatccggca
    16681 caatgccaaa ggtcaccagc ttgcccgctt cggcataagg catggcatta cgcacggcgg
    16741 cacggaacgc gtctttatcg gcaatcacat gatccgccgc caataccagc attaacgggt
    16801 cgctctccgg gctatgacgt tttgccgcca gcgccgccag tgcaatggca ggtgcagtgt
    16861 tacgccctgc cggttcgaga ataatgttct cggtgagttt gttcagttga cgcagctgtt
    16921 ccgcgacaat aaagcggtgc tgctcattgc aaatcaccac cgggctttcg cactccacgc
    16981 cgttcaggcg gcagatggtg gtttgcagca tggtgagatc gcctttcagg cataaaaact
    17041 gcttggggta aagtacgcgg gaaagcggcc ataaacggct accggagcca cctgccatca
    17101 caactggata gagtttcgac tgcgccataa ttatccccga atatcattta taaattgacg
    17161 taacacgttc tctttatcga gcgtgcgttc ggcatattca cgtgccaccg tgttatgttt
    17221 gggcagcagg agcgcctgac gaatccccgc caccagcgcc tcgaccgatt ccggttctac
    17281 gcaaaccgca atacccggaa aggtttcgca aagctgcccc agttctgtgt gggcctctgc
    17341 ggtaatcacc gcgttaccgc ctaccgccag aatattggtc agtttcgacg gcaatacggc
    17401 atctgccgcg ccgcgttttt acaccaccag atggcaatcg cccatcttca gcagtgcggg
    17461 taaagcgtca tacgattgca gcgggaaaaa ttgcatgttg cgcagtccac gctgctgcgc
    17521 cattttttcc agccgcgctt tgccgccgcc ctgcccgaca atggcaaaaa gcagcggttc
    17581 atcgcgcagg agatcggcag cttcaataac gttttccagc ccctgctttt caccaatatt
    17641 gccggagtaa agaatgattt ttttgttatc cggcaggcca agctggttac gaagggcatc
    17701 aacatcggcg tctgcaacat gctgaaaacg ggcaatttcc gaccagttgg ggaagaagat
    17761 gacgttatcc gccgccacgc ctttttcgat ggctttattc atcatcgaac gcgaaatcgt
    17821 ggaaacgtta tcgacgttat gcagtccgct acgttcgaac gccgttgcca gctgtgccac
    17881 tttgccgcct ttgccttttc cggcaaggcc cagccccagc atggcgtcca cttcgtaatc
    17941 ctgaatatgc agcacggtac gcgcaccaga gagcttcgcc agcaggcgca ttcccggcgt
    18001 gcaaaacagc gttggcacta cgccaataat gcgatccggc ttccagcgac gttgcgccat
    18061 cagtggaaag aaactgctga cggcaaaact gccgagatgc aacaagcgtt tcagggtgct
    18121 cggctgtttt ggcacgtaca gcgggcagcg ccacaccgtg gcggcccctt cttctcgttt
    18181 gtagcgccag gcggaatagt tctcacccac ctgccattgc gggtagtaag gcggtgcggt
    18241 aatgacccgc acctcatgac cttgtgccgc caaccattcc accatctcgc cggtgtattt
    18301 gccgatgccg gttaactccg gcgagtagtt aatgccgtag accagtattt tcataatccg
    18361 ggtactccgg cacgcttctc agcgagaaaa taggcgcggc tgttagcgtg aacatcgtta
    18421 ctggcgagca gcgcgtccgg cgtcagccag cggtaatcgt catgctgctc atccggcagt
    18481 aacagctctt cttccgctac tctgaagcga aaaccgagca ccacatagtg agtggtgaaa
    18541 tccgtgccgg agaagttatc gtcataaaaa tgctgccaga caccgtaaaa ctggcctgct
    18601 gttatcggca gacgcagccc cagttccgcc atcgtcagcc gctcaaatgc ggcttccagc
    18661 gtttcgtctt tctgcacgcg ccctcccggc acaaaccagt aaccctgcgc cgggcggttg
    18721 gttcttttgc caagcagaaa ctcgccgcga ctgttctcga caataaagtc gagagagaca
    18781 agcggtgtgg agcgcactac agtggcaaag tcttcctgac gtaaaaacat cattcccccc
    18841 gaaagcggtc ttgattctca aggaaccact ggtaagtgct ggcaagcccc gcttccagtg
    18901 agatttcgtg ataccagcca agctgatgca ggcgcgtcac atccagcagt ttgcgcggcg
    18961 taccatccgg tttgctggca tcaaaaacca cccgaccttt gtaacccacc actttggcga
    19021 tggtttgcgc cagctcgcgg atagtgcagt caacgcccgt gccgacgtta atgtgcgaca
    19081 gcatcggttg gctattctcc agccagactt catgcgccag ctccatgaca tgaatgctcg
    19141 ccgccgccat atcatcgacg tgcaggaatt cacgcatcgg tgtaccgctg ccccatacca
    19201 ccacgtccgg cgcattctgt gccgtcgcct cgtggaagcg acgcagcaat gctggtatca
    19261 catgggagtt actcgggtgg aagttgtcat gcggcccgta caggttggtc ggcatgacag
    19321 agcggtaatc gcgtccatac tggcggttgt aagattcgca cagtttgatc ccggcgattt
    19381 tggcaatggc gtaaggctcg ttggtcggct ccagcgtgcc ctgcaataac tcgctttctg
    19441 ccatcggctg ttttgccagt ttcgggtaga tgcaggacga tccgagaaac atcagtttgt
    19501 tcacgtcgtt ctgatgcgcg gcgtgaatga tgttgctctc aatcatcatg ttctggtaga
    19561 tgaaatccgc cggataggtg ttgttggcaa caatgccgcc cactttcgcc gccgccagat
    19621 agacctggtc aatgcgttcg ctggcaaaga aatcatgcac ggcgcggctg tccagcaggt
    19681 tcagttcatc gcgggtacgt aataccagct ccacatcacc gcgctgttcg agctgccgct
    19741 tgatggcaga accgaccatc ccacaatggc cagcgataaa aatgcgttgt ttacttatgc
    19801 ttatgactcc agcgcgatcg ccacgtcgta gccgtgagat ttcagcagag agtgtttttt
    19861 cgccgcttcg aggtcattag ccaccatttc agacaccatc tctctgaggg tgatttccgg
    19921 tttccagccc agtttttcgt gcgctttggt cgggtcgccg agcagcgttt caacttctgc
    19981 cggacggaag taacgcggat caacggcgat aatcacatca cccggtttaa cgcccggcgc
    20041 gtcatgcccg gtgacggaaa ccacaatgcc cttctcttca acacccgtgc cttcaaagcg
    20101 cagtttgatg cccagctgtg ctgccgccat ttccacaaac tgacgtacgg agtactgaac
    20161 gccggtcgcg ataacgaaat cttccggctg ttcctgttgc agcatcatcc actgcatttt
    20221 tacgtagtct ttggcgtggc cccagtcacg cagggaatcc atatttccga ggtacaggca
    20281 cgactccagc ccctgggcga tgttggcgat tgcgcgggtg attttgcggg taacgaaggt
    20341 ttcgccgcgg cgcggggatt catggttgaa gagaattccg ttacaagcgt acatgccgta
    20401 ggattcgcgg tagttaacgg tgatccagta ggcgtacagt ttggcgaccg catacggaga
    20461 tcgcgggtag aacggcgtgg tttctttctg cggaatttcc tgcaccagac catacagttc
    20521 agaggtggaa gcctgataga aacgagtttt cttttccaga ccgaggaagc ggatcgcctc
    20581 cagcaggcgc agcgtaccca tcgcgtcgac gtcagcggta tattctggtg actcaaaaga
    20641 gaccgcaacg tggctcattg cgcccaggtt gtacacttca tccggctgta cttcacgcaa
    20701 aatgcgcgtc aggttagagg tatcactcag gtcgccataa tgcagatgga atttcgggtt
    20761 gcaggtgtgc ggatcctgat aaatgtgatc cacgcgctcg gtgttgaatg acgatgcgcg
    20821 acgcttaata ccatgcacct cgtaaccttt ttccagcaga aactctgcca ggtaagaacc
    20881 gtcttgtccg gttacaccgg tgatgagagc gacttttgac atgtcttatt cctctgtatt
    20941 tttgaattta ttcagtttca acgcgttcgc gtatcaccac tgcgggattc ccccggcaaa
    21001 ccacatttgc cggaagcgat ttaaaaacac tgcttcgtgc acccacgacg gtgccgtcgc
    21061 cgattgtgac gcctggggca acaaagacat cggttgccag ccagcatttc tcgccaatca
    21121 caataggcgt ggcgttaatg gtgaaatgtt gacttgcatg gtcgtggcta ccggtgcata
    21181 aataactttt ttgcgatatc accgaatgtg cgccaatggt tatttcaccg agggtatata
    21241 aattgacgtc atcgccaacc cacgcgtaat cacctaaggt taatttccac ggataggtaa
    21301 tttttactga cggacgaata actacgtttt ttcctatttt tgctccgaat aaacgtaata
    21361 aaaaagcccg ccagcgatac aatacttgtg gcgaccaggc aaatattgtt gcctgtactg
    21421 cccaccataa ttgcacttta atagcgttgc cgccccggaa ccctttcggc accgagaaac
    21481 cgcttaaatc ttgcattggt tttccttata ttcagacttt gttatataag gctttagtct
    21541 tacctgtcgt acgttggcgt aaatgccagg ataattctgc ccagaagcca ggtacatgta
    21601 atatctgccg ttggactttt ttggcgtcag cac
//
LOCUS       DADLZM010000060        21504 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLZM010000060 DADLZM010000000
VERSION     DADLZM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.060"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNY73_002182"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNY73_002182"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY8048192.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNY73_002183"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNY73_002183"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY8048193.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEMEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JNY73_002184"
     CDS             complement(3121..3420)
                     /locus_tag="JNY73_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048194.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JNY73_002185"
     CDS             3595..4590
                     /locus_tag="JNY73_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8048195.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JNY73_002186"
     CDS             complement(4632..5069)
                     /locus_tag="JNY73_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY8048196.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JNY73_002187"
     CDS             complement(5115..5456)
                     /locus_tag="JNY73_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048197.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNY73_002188"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNY73_002188"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY8048198.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMLALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNY73_002189"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNY73_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="JNY73_002190"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="JNY73_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8048199.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     TKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEKEL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="JNY73_002191"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="JNY73_002191"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY8048200.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="JNY73_002192"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="JNY73_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY8048201.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13864
                     /locus_tag="JNY73_002193"
                     /pseudo
     CDS             12686..13864
                     /locus_tag="JNY73_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(13972..14796)
                     /locus_tag="JNY73_002194"
     CDS             complement(13972..14796)
                     /locus_tag="JNY73_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY8048202.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="JNY73_002195"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="JNY73_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="JNY73_002196"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="JNY73_002196"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY8048203.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRSLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="JNY73_002197"
     CDS             complement(18728..19201)
                     /locus_tag="JNY73_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8048204.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNY73_002198"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNY73_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY8048205.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20351..21349
                     /gene="yiaK"
                     /locus_tag="JNY73_002199"
     CDS             20351..21349
                     /gene="yiaK"
                     /locus_tag="JNY73_002199"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY8048206.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21361..>21504
                     /locus_tag="JNY73_002200"
     CDS             21361..>21504
                     /locus_tag="JNY73_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY8048207.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5406 a         5488 c         5277 g         5333 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccatttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcga
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacacc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagaa agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctcgacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc agcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag
    20341 gaagcctagg atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat
    20401 ttcacgcggc gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac
    20461 cgaatccggc gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga
    20521 aaacggcgat atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga
    20581 acagtgggat gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc
    20641 cattgaactg gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg
    20701 gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg
    20761 gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa
    20821 cccgctgatc gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgtcgat
    20881 gttctcttac ggcatgttag aagttaaccg cctggcaggc cgtcagctcc cggtcgatgg
    20941 tggctttgat gatgagggca atttgaccaa agaacctggc gttatcgaga agaatcgccg
    21001 cattttgccg atgggctact ggaaaggttc tggcatgtcg attgtgctgg atatgatcgc
    21061 tactctcctt tccgacggcg catcggttgc cgaagtcacc caggacaaca gcgacgaata
    21121 cggcatttca caaattttta ttgccattga agtggacaag cttatcgacg gtcccacccg
    21181 cgatgccaag ctgcaacgta tcatggatta cgttactacc gctgaacgcg ctgacgaaaa
    21241 ccaggccatc cgcttacccg gccacgaatt tactaccctg ctggccgaaa accgccgtaa
    21301 cggcatcact gttgatgaca gcgtgtgggc caaaatccag gcgttatgag gagataaatc
    21361 atgatttttg gacatatcgc gcagcccaat ccgtgccgtt tgcccgccgc cattgaaaag
    21421 gcgctcgatt ttctgcgcgc taccaacttc aacgtcctgg agccgggcgt tgtcgaaatc
    21481 gacggcaaga atatttatgc gcaa
//
LOCUS       DADLZM010000061        21400 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLZM010000061 DADLZM010000000
VERSION     DADLZM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.061"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="JNY73_002201"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="JNY73_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY8048208.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="JNY73_002202"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="JNY73_002202"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY8048209.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="JNY73_002203"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="JNY73_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY8048210.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="JNY73_002204"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="JNY73_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY8048211.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="JNY73_002205"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="JNY73_002205"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY8048212.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="JNY73_002206"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="JNY73_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY8048213.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11351
                     /gene="ftsK"
                     /locus_tag="JNY73_002207"
     CDS             7245..11351
                     /gene="ftsK"
                     /locus_tag="JNY73_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY8048214.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAVDPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11506..12117
                     /gene="lolA"
                     /locus_tag="JNY73_002208"
     CDS             11506..12117
                     /gene="lolA"
                     /locus_tag="JNY73_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY8048215.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDEQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12128..13471
                     /locus_tag="JNY73_002209"
     CDS             12128..13471
                     /locus_tag="JNY73_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY8048216.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13562..14854
                     /gene="serS"
                     /locus_tag="JNY73_002210"
     CDS             13562..14854
                     /gene="serS"
                     /locus_tag="JNY73_002210"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY8048217.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15094..17538
                     /gene="dmsA"
                     /locus_tag="JNY73_002211"
                     /pseudo
     CDS             15094..17538
                     /gene="dmsA"
                     /locus_tag="JNY73_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            17549..18166
                     /gene="dmsB"
                     /locus_tag="JNY73_002212"
     CDS             17549..18166
                     /gene="dmsB"
                     /locus_tag="JNY73_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY8048218.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18168..19031
                     /locus_tag="JNY73_002213"
     CDS             18168..19031
                     /locus_tag="JNY73_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY8048219.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19067..19693)
                     /locus_tag="JNY73_002214"
     CDS             complement(19067..19693)
                     /locus_tag="JNY73_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8048220.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20008..21156
                     /locus_tag="JNY73_002215"
     CDS             20008..21156
                     /locus_tag="JNY73_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048221.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGSVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         5281 a         5376 c         5660 g         5083 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaact tgtgcagtat
      121 atctacatcg agacaagtta tggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag caccgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tttgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcac aaaagacaaa cagggcaatt aatgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata acgctgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat tttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cacgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcggccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacctgca gccagcgtca ccccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gtcgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgccg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta gcggtgctgt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca atacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg cgccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgta tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtct agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgtcgaccc ggacgacgtc ctatttttgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgcaa ctgcgccgga acagcctgcg caacagccgt
     8581 attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc
     8641 aatccatttt tgctccacag tctacatacc agactgagca aacttatcag cagccagccg
     8701 ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg
     8761 aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg
     8821 aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac
     8881 cggttaaaga accagaaccg atcaaatctt cgctgaaagc accttctgtt gcagcagtac
     8941 ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac
     9001 tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg
     9061 gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc
     9121 gcgtgccaac tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca cagcgtgcgg
     9181 cggaagaaaa agcccgtgaa gcccagcgca atcagtatga ttctggcgat cagtataacg
     9241 atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc
     9301 agcaacgcta tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg
     9361 ctgcggcaga ggctgaactg gctcgtcagt ttacgcaaac tcaacaacaa cgttattccg
     9421 gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga
     9481 aagcgttgct ggatgatggt ccacacgaac cgctgtttac gccaattgtt gaacctgtac
     9541 agcagccgca acaaccggtt gcaccacagc agcagtatca gcagccacaa cagccggttg
     9601 cgccacagcc gcagtatcag cagccacaac agcaggttgc gccgcagcca caatatcagc
     9661 agccgcaaca accggttgcg ccacagcagc aatatcagca gccgcaacaa ccagttgcgc
     9721 cgcagccgca gtatcaacag ccgcaacaac cggttgcacc gcagccgcag tatcagcagc
     9781 cacaacagcc agttgcgcca cagccgcagg ataccctgct tcatccgctg ttgatgcgta
     9841 atggcgacag ccgtccgttg cacaaaccga cgacgccgct gccttctctg gatttgctga
     9901 caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggcgcgtc
     9961 tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgttaat tactctccgg
    10021 ggccagttat cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt
    10081 ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag
    10141 ttattcctgg caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt
    10201 atctgcgcga agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg
    10261 tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact
    10321 tgttggttgc ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga
    10381 gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc
    10441 tggagctttc ggtttatgaa ggcattccgc acctgttaac ggaagtcgtt actgatatga
    10501 aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgg tataaactga
    10561 tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc
    10621 gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc
    10681 atccggtgct gaaaaaagaa ccatacattg tcgtgttggt tgacgaattt gccgacctga
    10741 tgatgacggt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg
    10801 ccgcgggtat ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc
    10861 tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac
    10921 gtaccattct tgatcaggct ggcgcggaat cactgctggg tatgggggat atgctctact
    10981 ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag
    11041 ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca
    11101 cctccgacag cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc
    11161 cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg
    11221 tacagcgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag
    11281 cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc
    11341 cgtttgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat
    11401 caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag
    11461 tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgct
    11521 atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc
    11581 gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg
    11641 actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca
    11701 aacttattca actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa
    11761 acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc
    11821 accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac
    11881 aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg
    11941 aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag
    12001 caggacgagc agcgcagcag ttatcagctg aaatcccagc aaaatggggc tgtggatgca
    12061 gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg
    12121 cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc
    12181 gcgtatgcgg ccagaaaatt tagcacagta tatcggtcag caacatttgc tggctgcggg
    12241 gaagccgttg ccgcgcgcta tcgaagccgg gcatttgcat tctatgatcc tctgggggcc
    12301 accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt
    12361 ggaacgtatt tctgccgtca cctctggcgt gaaagagatt cgcgaggcga tcgagcgcgc
    12421 ccggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt
    12481 caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat
    12541 tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg
    12601 tgtttatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat
    12661 ggaagacaaa acccgtggct atggtggtca ggatattgtt ctgccagatg atacacgacg
    12721 tgccattgca gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat
    12781 ggcggatatg gccgaagtct atgatagcgg taagcgggtc ctgaagcctg aattactgac
    12841 cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct
    12901 aatttccgca ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta
    12961 tgcgcgaatt attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat
    13021 tgcgtctgaa gacgtcggta atgccgatcc acgggcgatg caggtggcaa ttgcggcctg
    13081 ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt
    13141 ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc
    13201 cgatgctcgc gaacgcccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa
    13261 attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc
    13321 ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc
    13381 gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg ctgaacagga
    13441 tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt
    13501 atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag
    13561 catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg
    13621 ccggggcttt aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt
    13681 gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca
    13741 ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga
    13801 agagctggat gcagcaaaag ccgagctaga tgctttacag gctgaaattc gcgatatcgc
    13861 gctgaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa
    13921 cgttgaagtc agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt
    13981 gacgctgggt gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc
    14041 ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat
    14101 gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa
    14161 ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac
    14221 tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt
    14281 tccgctgact aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat
    14341 gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg
    14401 tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccagaaga
    14461 ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg
    14521 cctgccgtac cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac
    14581 ttacgacctg gaagtatgga tcccggcaca gaacacctac cgtgagatct cttcctgctc
    14641 caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgccgcagca agtcggacaa
    14701 gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt
    14761 tgcagtaatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg
    14821 tccgtatatg aacggactgg aatatattgg ctaataccca atttttctga atctaaaaag
    14881 cgcctttagg gcgctttttt tgtctccctt tgataccgaa caataattac tcctcactta
    14941 cacgtaatac tactttcgag tgaaaatcta cctatctctt tgattttcaa attattcgat
    15001 gtatacaagc ctatatagcg agctgctata gaaataatta cacaatacgg tttgttactg
    15061 gaatcaatcg tgatcaagct tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta
    15121 ttggctgctg aggagagtcg ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca
    15181 atggccagca gcgcattaac attacctttt agtcggattg cgcacgctgt cgatagcgcc
    15241 attccaacaa aatcagacga aaaggttatc tggagcgcct gtacagttaa ctgtggtagt
    15301 cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca aatatgtcga aacggacaat
    15361 accggcgatg acaattacga cggcctgcac taggttcgcg cctgcctgcg tgggcgttcc
    15421 atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc cgatgaaacg agtcggggcg
    15481 cgcggtgaag gcaaattcga gcgcattagc tgggaagaag cctacgacat catcgcgacc
    15541 aatatgcagc gcctgatcaa agagtacggc aacgagtcta tctatctgaa ctatggcacc
    15601 ggtacgctgg gcggcaccat gacccgctcc tggccgccgg gaaataccct ggtcgcgcgc
    15661 ctgatgaact gctgcggcgg ttatcttaac cattacggtg actactcttc cgcgcaaatt
    15721 gcggaaggtt tgaaccatac ctacggcggc tgggcagatg gtaacagccc gtcggatatc
    15781 gaaaacagta aattggtagt gctgtttggt aataacccag gcgaaacgcg aatgagtggc
    15841 ggtggggtga cttactatct tgaacaggca cgccagaaat ctaatgctcg cataatcatc
    15901 atcgatccgc gctataccga caccggtgcc gggcgcgaag atgagtggat ccctattcgt
    15961 ccaggaacag atgccgcact ggttaacggt ctggcgtacg tcatgatcac tgaaaacctg
    16021 gtggatcagg cattcctcga taaatattgc gttggctatg acgagaaaac cctgcccgcc
    16081 agtgcgccga aaaatggtca ttataaagct tatattctgg gcgaagggcc agatggcgtg
    16141 gctaaaacac cggaataggc ctcgcaaatc accggtgttc cggcagacaa aatcatcaaa
    16201 ctggctcgtg aaatcggcag taccaaaccg gcgtttatca gccagggatg gggcccgcag
    16261 cgtcacgcta acggtgaaat cgcaacccgt gctatctcga tgctggcgat tctgaccggt
    16321 aacgttggta ttaatggagg gaacagcggc gcgcgtgaag gttcatacag cttaccgttt
    16381 gtccgtatgc cgaccttgga aaacccgatc cagaccagca tttcgatgtt tatgtggacc
    16441 gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg atggtgtgcg cgggaaagat
    16501 aagctggatg tgccgatcaa aatgatctgg aactatgccg gtaactgcct gattaaccag
    16561 cattctgaaa tcaaccgtac ccatgaaatc ctccaggatg ataagaagtg cgagctgatt
    16621 gtggttatcg actgccacat gacctcatcg gcgaaatatg ctgacatcct gctgcctgac
    16681 tgcaccgctt ccgaacagat ggactttgca ctggatgcat cctgcgggaa tatgtcttac
    16741 gtgattttca acgatcaggt gattaaaccg cgctttgaat gtaagaccat ctatgaaatg
    16801 accagcgaac tggcaaaacg tcttggcgtt gagcaacagt ttactgaagg ccgtacccag
    16861 gaagagtgga tgcggcatct gtatgcccag tcgcgggaag cgattcctga actgccaacg
    16921 tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg acccacaagg gcatcacgtt
    16981 gcttataaag ccttccgtga agatccgcag gcaaacccac tgactacgcc atcgggcaaa
    17041 attgagattt attcgcaggc gctggctgac attgccgcta cctgggaatt gccagaaggc
    17101 gatgtgatcg atccactgcc gatctacacg ccgggctttg aaagttatca ggatccgctg
    17161 aacaaacagt atccgctgca gcttacaggt ttccactata aatctcgcgt tcactcaact
    17221 tacggcaacg ttgatgtgct gaaagcggct tgccgtcagg aaatgtggat caacccgctt
    17281 gatgcccaaa aacgcggtat ccacaacggc gataaagtca ggatctttaa cgatcgtggt
    17341 gaggttcata ttgaggcgaa agtgacgcca cgaatgatgc cgggcgtggt cgcactgggt
    17401 gaaggcgcct ggtatgaccc ggatgcaaaa cgtgtcgata agggcggttg tatcaacgta
    17461 ctgaccactc aacgtccgtc tcctctcgct aaggggaatc cgtcacatac aaaccttgtt
    17521 caggttgaaa aggtgtaagg agtaaccgat gacaacccag tatggatttt ttattgattc
    17581 cagccgttgc accggttgca aaacctgcga gctggcttgt aaagactaca aagatttgac
    17641 gccagaagtc agtttccgcc gcatttatga atatgctggc ggcgactggc aggaagataa
    17701 cggtgtctgg caccagaacg tgtttgccta ctatctgtcg atttcgtgca accactgcga
    17761 agatccggct tgtactaaag tctgcccgag cggtgcgatg cataaacgcg aagatggttt
    17821 tgttgtggtc gatgaagatg tatgcattgg ctgccgttac tgtcatatgg cttgcccgta
    17881 tggcgcaccg caatataacg aaacgaaagg ccatatgacc aaatgcgatg gttgttatga
    17941 ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct tgcccgctgc gggcgctgga
    18001 tttcggccct atcgacgagc tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt
    18061 gccgcgagct cactttacca aaccgaatat tgtgatcaaa cccaatgcca atagccgccc
    18121 gacaggggat accactggct atctggcaaa cccgaaggag gtgtgagatg ggaagtggat
    18181 ggcatgaatg gccgctgatg atcttcacgg tcttcgggca atgtgtggcg ggtggtttta
    18241 tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta
    18301 tcgcctgcat gtttggctta tgggtactga tgggcattgg ctttatcgcc tctatgctcc
    18361 atcttggttc accgatgcgc gcctttaact cgctcaaccg ggtaggggct tcagcactca
    18421 gtaacgaaat cgccagcggt tcgatctttt ttgctgtagg cggcatcggc tggttgctgg
    18481 caatgctgaa aaagctgtca ccggcattgc gtacgctatg gctggtggtg acgatggttc
    18541 ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa cagcattgat accgttccga
    18601 cctggtacag catctggacg ccgatgggct tcttcctgac gatgttcatg ggcggcccgt
    18661 tactgggtta cctgttgctg agtctggctg gcgtcgatgg ctgggcgatg cgtctgctgc
    18721 cagccatttc tgtactggca ctggtggtga gtggcgtgat gtcagtgatg cagggcacag
    18781 agctggcaac tattcatagt tctgtgcagc aggccgcagc gctggtgccg gactatggtg
    18841 cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct gtgcttgtgg attgcaccac
    18901 agctaaaagg ttatcagcct gcggttccgc tactatccgt ctcattcatt ctgcttctgg
    18961 caggggaatt aatcggtcgc ggcgtattct atggtttgca tatgaccgtg gggatggccg
    19021 tcgcaagcta atcagtaaca accggggttt cggccccggt tctcttttat ttctgcttcg
    19081 ttaacgtgtc ataactggtc atcaaattac gataatcagg aatatggtta gagaacaacg
    19141 tcgccagtcc ttcaatatca ttacgccagt cacggtgcag ctcgcaagcc acgccgaacc
    19201 atgtcatcaa ttgcgcacca gcttgcgaca tgcgatccca cgccgaatgc cgggtaattt
    19261 cattaaaggt ccctgatgca tcagtcacga caaagacatc aaacccttct tcaatggctg
    19321 aaagcgccgg gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt
    19381 tacctgtcgc tttgactgct tttacaaaat cttcgttatc ccaggcgtta atattgcccg
    19441 ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag ttctagaact aatgggccgt
    19501 taggaccggt ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagccaggt
    19561 cacccagcgc cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa
    19621 gtaaaccagc ctggtgatca acaagcaaaa cggcagcatc atttttatca agacgaacat
    19681 acggtttggt catcctcttc tcctttcgag agtcagtggt acgtctgaaa tcggtcagac
    19741 gcgatgcatt gctctgaaag catagacggg aaatatgagt ttgctgtgac catgaaattt
    19801 ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat aaaaacaatc agatgagatt
    19861 aatcatctct tcagcattag cgttatgaat gagtaatata aatcgataat accgccgctg
    19921 cgatatgcgc gtattttcac ccattgacaa tgtttttggc ggtggcatga tgcgcatgaa
    19981 atttgaactt cctcacggtt ttaattcatg tccacgtata cccggcctgt catgcttttg
    20041 ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt taaatacact cgtgccgctt
    20101 tggctcgccc aggaacacat gtccacatgg caggtaggcg ttgtcagctc atcctatttt
    20161 accggcaacc ttgtcggtac attgctgaca gggtatgtca ttaaacgcat tggctttaac
    20221 cgcagctatt atctgtcctc cttcattttt gctgctggct gtgccggcct tggcctgatg
    20281 attggtttct ggagctggtt ggcgtggcgt tttgtcgcgg gcgtcggctg tgccatgatt
    20341 tgggtggttg ttgagagcgc gctgatgtgc agtgggacgt cacgtaaccg tgggcgtttg
    20401 cttgctgcgt atatgatggt ttattacgtg ggaacgtttt taggccagtt actggtcagc
    20461 aaagtctcaa ccgagctgat gagcgtattg ccgtgggtta caggtttgac attggcaggg
    20521 atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg ctgaaaacca tgattcgacg
    20581 tcaattacgg caatgctaaa actccgtcag gcgcggcttg gcgtgaatgg ctgcattatc
    20641 tcaggaagcg ttctgggatc tctatatggc ctgatgcctc tgtacctcaa tcacaaaggg
    20701 gtgagcaatg ccagtattgg tttctggatg gcggtactgg tcagtgcggg tatcctcgga
    20761 caatggccga ttggacgtct ggcggataag tttggtcgac tgctggtttt gcgtgttcag
    20821 gtctttgtcg tcattctcgg cagtattgcg atgcttagcc aggcggcgat ggccccagcg
    20881 ttattcatcc tcggtgccgc tggctttacg ctatatccgg tggcgatggc ctgggcttgt
    20941 gagaaagttg aacatcatca actggtggcg atgaaccaag ccttactgtt gagctatacc
    21001 gtgggaagtc tgcttggccc gtcatttacc gctatgctaa tgcagaattt ctccgataat
    21061 ttattgttta tcatgatcgc cagcgtatcg tttatctatt tgctgatgct gcttcgcaac
    21121 gccggtcata cgccgaaacc cgttgctcac gtgtaaatga attcaagcaa agtgtgaact
    21181 tactgtttca cactctgctt ttttgtttct tctatctgac ttgctttttt ccaaatttta
    21241 ttcgtttaaa aataaaatgt gcagcaggtt ataattttgc atttcgctat ttccgcactt
    21301 cttatttgcc gcgcataatc cctcgtttta cagatgcccc tttaattttg gcgaaggatt
    21361 tgtctatggc tgggaatgtt caggaagtaa gcgccccatc
//
LOCUS       DADLZM010000062        21264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLZM010000062 DADLZM010000000
VERSION     DADLZM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.062"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            15..923
                     /locus_tag="JNY73_002216"
     CDS             15..923
                     /locus_tag="JNY73_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048222.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTSQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLATLTSPLTSTTPVAENVE"
     gene            1162..1221
                     /locus_tag="JNY73_002217"
     CDS             1162..1221
                     /locus_tag="JNY73_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY8048223.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1538..1597
                     /gene="ibsE"
                     /locus_tag="JNY73_002218"
     CDS             1538..1597
                     /gene="ibsE"
                     /locus_tag="JNY73_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY8048224.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="JNY73_002219"
     CDS             complement(1717..3150)
                     /gene="hldE"
                     /locus_tag="JNY73_002219"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY8048225.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="JNY73_002220"
     CDS             complement(3198..6038)
                     /gene="glnE"
                     /locus_tag="JNY73_002220"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY8048226.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6061..7362)
                     /locus_tag="JNY73_002221"
     CDS             complement(6061..7362)
                     /locus_tag="JNY73_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY8048227.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWLYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLIQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7604..8224
                     /locus_tag="JNY73_002222"
     CDS             7604..8224
                     /locus_tag="JNY73_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY8048228.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPNRKRKDRWMN"
     gene            8288..9526
                     /gene="cca"
                     /locus_tag="JNY73_002223"
     CDS             8288..9526
                     /gene="cca"
                     /locus_tag="JNY73_002223"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY8048229.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="JNY73_002224"
     CDS             complement(9689..10510)
                     /gene="bacA"
                     /locus_tag="JNY73_002224"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY8048230.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10601..10969)
                     /gene="folB"
                     /locus_tag="JNY73_002225"
     CDS             complement(10601..10969)
                     /gene="folB"
                     /locus_tag="JNY73_002225"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY8048231.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11074..11691
                     /gene="plsY"
                     /locus_tag="JNY73_002226"
     CDS             11074..11691
                     /gene="plsY"
                     /locus_tag="JNY73_002226"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY8048232.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11704..12635)
                     /gene="ttdR"
                     /locus_tag="JNY73_002227"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11704..12635)
                     /gene="ttdR"
                     /locus_tag="JNY73_002227"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12842..13685
                     /gene="ttdA"
                     /locus_tag="JNY73_002228"
                     /pseudo
     CDS             12842..13685
                     /gene="ttdA"
                     /locus_tag="JNY73_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13682..14287
                     /gene="ttdB"
                     /locus_tag="JNY73_002229"
     CDS             13682..14287
                     /gene="ttdB"
                     /locus_tag="JNY73_002229"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY8048233.1"
                     /translation="MKKILTTSIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14335..15798
                     /locus_tag="JNY73_002230"
     CDS             14335..15798
                     /locus_tag="JNY73_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY8048234.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYPPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JNY73_002231"
     CDS             complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JNY73_002231"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY8048235.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17092..17307
                     /gene="rpsU"
                     /locus_tag="JNY73_002232"
     CDS             17092..17307
                     /gene="rpsU"
                     /locus_tag="JNY73_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY8048236.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17418..19163
                     /gene="dnaG"
                     /locus_tag="JNY73_002233"
     CDS             17418..19163
                     /gene="dnaG"
                     /locus_tag="JNY73_002233"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY8048237.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19358..21199
                     /gene="rpoD"
                     /locus_tag="JNY73_002234"
     CDS             19358..21199
                     /gene="rpoD"
                     /locus_tag="JNY73_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY8048238.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5127 a         5878 c         5462 g         4797 t
ORIGIN      
        1 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
       61 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      121 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      181 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg
      241 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
      301 cagaagcgcg tgctaacctt gcactcgcag aagcagtaag tgcgcaacaa aacgtagaaa
      361 ccactagcca gaccgcagaa gccgatcgtg ctaaacaagt tgccctaatc gctgccgcgc
      421 aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg
      481 cagagatgca ggcggcggct atcgttgagt tagccgaagc tacacgtaaa aagggtctgg
      541 cggaagcaga agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa
      601 ccagccttaa attcaaactg gccttgttgc aggcgctacc tgcggtaata gaaaaatccg
      661 ttgagccgat gaaatctatt gacggtatca agattattca ggtcgatggt ctgaatcgtg
      721 gcagcgctgc gggtgatgca aacacgggta atgtgggggg cggaaaccta gcggaacaag
      781 cattatcagc cgctctctct taccgcacac aggctccgct gattgactcc ttgctcaatg
      841 aaattggcgt ttcaggcggt tcactggcga cattgacttc acccttaacc tcaacaactc
      901 ccgtcgccga aaacgtagaa taaattttca gctatgaacg tccctggcac aaaaagccgg
      961 ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt gcaacgtttt
     1021 gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag cgttgcgcga ggtccggcgc
     1081 tcatcaacaa tcggggagtc agcaaagggc tgaaacggga aagcccctcc cgaggaaggg
     1141 gccttacaga aggaaagggt tatgatgaag ctcgtcatca tactgattgt gttgttactc
     1201 gtaagtttcg cagcttatta acagccaatc agagggggga gaaatcctcc ctcacccttg
     1261 ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa cacatatccc
     1321 tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgctcag
     1381 gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt
     1441 gcgcggggtc cggcgctcat caacaatcgg ggagtcagca aggggctgaa acgggaaagc
     1501 ccctcccgag gaaggggcct tatagaagga aagggttatg atgaagttcg tcatcatact
     1561 ggttgtgttg ttactgttaa gtttcccgac ttactaacaa ctgatcagag gggggagaaa
     1621 tcctccctta cccttgttcc tttactttag gttgaaaaaa caacagcgtc aataggcctg
     1681 cctgctacga agcgagatct gtgaaccgct ttccggttag ccttttttat cctgttggat
     1741 cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca gcacttcgcc
     1801 gccgttggcc cagacttctt tactcccggc gatctcttct ggtttatagt cgccgccctt
     1861 caccagtaaa tctggcagga tcccggcgat caagcgctgc ggcgtgtctt cttcaaacga
     1921 caccacccag tcgaccgctt ccagtgcgcc cagcacaatc atacgctgtt cgagcgggtt
     1981 aacggggcgg gaatcccctt tcagccgttt ggtggaggca tcgctgttca cagcgacaat
     2041 caagcggtca cccagtttgc gggcatttgc cagatagaag acgtgtccgg cgtgcaggat
     2101 gtcaaagacg ccattggtca tcaccacttt ttcaccgcgt ttacgcgctg ccgctacggc
     2161 cagcttcagt tcctcttcgg tcatcacgcc aaagccggta tctgcgcgcc cacgcactgc
     2221 attttccagc tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca ccacgccagc
     2281 cgccgcattg gcaaagaagc aggcttcttc cagcgaatta cccgctgcca gcgttgccgc
     2341 caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca tacacttcct gcgcctgggt
     2401 tggcatatgc agcggcgctt tacccggttg cagcagcgac ataccctgtt cggaacgggt
     2461 cactaacaga gccgagagtt cgtaatcggc aatcagtttc atgccgcgct caacaatctc
     2521 ttcttcggtc ttacatttac cgacaacagc ttcaaattcc gagagattcg gtgtcagcag
     2581 cgtagcgccg cggtagcgct caaaatcggt accttttgga tcaatcagca ccggaacgcc
     2641 cgctttacgc gccagttgga tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc
     2701 agaaagcacc agcgcgccaa tcgaactcag cgcctgatta atccgctcgt gcagcggctg
     2761 cggatcaaca ccttcgaaac cttcttcaaa atccagacgg atcagctgtt ggttgcggga
     2821 aagtacccgt aatttggtga tggtcggatg cgtcggtaca gaaacgaagt cgcatttgac
     2881 gttgacgtcg gccagagatt tactcagcgc gcgcgctgca tcgtcaatgc ccgtcaaccc
     2941 gaccaggcgt gcattagcac cgagagaagc gatattcatc gccacgttag ccgcgccgcc
     3001 cggacgttct tcgatggtat tcactttaac cacgggcacc ggcgcttccg gcgagatacg
     3061 actggtgggg ccgtaccagt aacgatccag catcacatca ccaaccacca tcactcctgc
     3121 acgttcaaac tctggcagcg ttactttcat tcctgtctcc tgagagattc aaaatttgcg
     3181 cgcgataata ccatacttca ttcttccacc agccacttct gccagcttgc ccgcaccaga
     3241 tcacgctctg cggtgaagca atcctccgac acatggcccg gcaattcctg taatgccaga
     3301 tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca gcgccatcgc ttcctgctct
     3361 tccataatgt cgttttgcgc cagcagctcc agaatacgca cgttgtctga ccagcgcgtt
     3421 aacttcggtt tttcatgagc gtagcgcaac accagatatt gggtaataaa ttcgatatcg
     3481 gtaattccgc cttcatcggc tttgatatca aagcgattac gatgtttatt gccgagatga
     3541 gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca gagttttacc ttctcgcggc
     3601 agtgtcataa tctcacgacg caccgcgtca aagtgcgcag tgagctgcgg atcgccgtac
     3661 actacacgcg cacgcaccag cgcctgatgt tcccacgtcc aggcctcgtt tttctgataa
     3721 tcggcaaatg ctgctgcgga tgtcaccagc attcccgccg ccccggacgg acgcagtcga
     3781 gcatccactt catacaaaat gccggaagag gtacgcgtac tgaacagatg cataatgcgt
     3841 tgcgccagac gcagataaaa ctgccgcccg tcgatttccc gctcgccgtc agtcatcgca
     3901 tccattgggc aatcatggag gaagataagg tcaagatcgg aactgtagcc taactcccag
     3961 ccgcccagct tgccgtagcc gaccaccgca aaaccgcgcc cttcacgttc gttgaggtga
     4021 tttggcttac cgtagcgggc caccatttga ccccacgcct gctgaacgac ggcatctatc
     4081 atagcttctg ccagccaggt taagtgatcg ctcactttca ttaccggtag cgtaccggcg
     4141 atatccgctg ccgcaatgcg taacagctgc gcctgtttga actgacgcag cgcctcaagc
     4201 tgttgttctt cgtcatcttc cggcacgcgc agcaaatact ggcgcagttc atcacggtag
     4261 gcatcggtcg ccgtcggctg gtaaagggta tttggatcga gcaattcatc cagcaataat
     4321 ggataacgcg ctagctggct ggcaatcatc ggcgatgcgg cacacagaga aatcaaatgt
     4381 ttaagcgccg cagggaattc actgagcaat tctaaatagg ttgtgcgggt aacaatcccc
     4441 accagcaagg cggtaatgcg cgacagcgtg acagcagcgt cttcacgcgc acagacatca
     4501 cttagcagat gtggcatcag atggtcgagc acctgacgtc ctcgcggccc gatagtacgc
     4561 ttatccagct ctttgcggaa atcagcaatc agcgttagga cctgtttgcg atcatcctcg
     4621 ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca acgcatcctg ccacagctca
     4681 cgccactgtt ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca
     4741 ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcag ttgcggccag
     4801 tcagcaaagt ccatcgccca cgccagccgc gcacggttaa gctcatcaga aggaagcgtt
     4861 tgggtttgtt cgtcgttaat gctttgcagc aggttttcca gacgccgcag gaacagatat
     4921 gccactcgca attgttcagc atcgttttca gaaagcagat gcagcgcggc aatggcgctg
     4981 agcgttggca gtaaagagcg cgactgcagc aacggttcgc gcccaccgcg aatgagctgg
     5041 aacacctgaa cgataaattc aatttcgcga atgccgcctg cgccgagttt gatattgtcg
     5101 gtcaaaccac gtcgacgcac ttcacgggca atcatccctt tcatgttgcg cagcgactga
     5161 atcacgctga aatcgatgta acgacggaaa acaaacgggc gcagcatcgc acgcaactcg
     5221 ttagcataga cgccttcgct atcgcccata atccgcgctt tgaccatcgc gtaacgctcc
     5281 cagtcgcgtc cctgctcctg gtaataatcc tccaacgcgg caaagctcag caccagcggg
     5341 ccactttcgc caaacggacg cagtcgcata tccacgcgat agacgaagcc atcctgcgtt
     5401 ggttgatcca gcactttaat cagccgctgc cccatgcggg taaaaaactg cgcgttatcc
     5461 agttcccggc gtccgccctg cgtacaacca tgttccggcc aggcaaaaat cagatcgata
     5521 tcagaggaga aatttagttc cccgccgccc agcttaccca tgcctaagat cagcagcggt
     5581 tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc ggcagcaggc gtcatacagc
     5641 cagtcacgcg ccgcaacaat cagcgtctcc gccagataac tgagctgctg caatatgctc
     5701 tcttcagtaa ccagtgccag cgtttgcgcc catgcgatgc gcaccataat gcgccgccgg
     5761 aatagccgca gctcgcgcat taacccggct tcgtcgctca cattactgag cgcctcctgc
     5821 aaccacacgc tgtaatgttg ccattcgtca gcctgtggcg gttggctttc cagttccgtc
     5881 agccactctg gatgcgcaat cacgctgtcc tgcacaaaat cactgaaagt aagtactgac
     5941 ttcgcctgtg cactaagtga ttcctcggct aaaagctctg gcagccgctc aacaacggtc
     6001 tgccagtact gctgtaacgg tgaagagagc ggcttcataa gcgattttat ccttgcctga
     6061 ttaacgtttt ccgctgtgca accagaacgg ttcctgattg tttgcctcat tacggaaatg
     6121 ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc agcccctgcc agttctccag
     6181 ccaggcttgc gcgacgacag gatcatagta acccgccagc aacagtattg agtcaatatc
     6241 acgcatcagg cgtggcaact ggtcacggta gcgatcgcct aacggctggc agaaaacgct
     6301 tttcagttca gcggcatggc gggaaagatg gatatcggca aagcgtttga aggagtcgct
     6361 aattttgccc tgcgctttgg catctaaaaa cggctgccat gctttgctta ccagccattc
     6421 ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag accgccgtca cggcagaaac
     6481 cgcagaagcg atggtcgctt cgcattgaat cagcaaatca cgtaagtgag tgctcgcttt
     6541 acgcggcaca atgccaccga acagcatcag tgtatgacgg actaggctaa tggctgccag
     6601 cacctgctct ttcgccgcat cgttgccgcg tacccacagt tcttcatgat acagccattg
     6661 cgctaacacc agctcgagcg ccgcttccag cccctgttcc acatcggctt ttgccgcaac
     6721 atgcaaaatg gtggtcggtt tgatttcacg cgccggattg ccctgcgcca gatgatagcc
     6781 acgcgccgct ttgctcaggc tgccctggcg taatccggtt tgcgatacca gttggttcgc
     6841 cagtttcagc accgcgcgcg tgtcgccgct aagcagctcc agttccagct cgcagatagg
     6901 ctcagcaaat tcacccgctt tcacttcccc ctggtcgaga gcgatttcaa ttcggctgcc
     6961 atcgatctct actagccatt tttcgcgata aaaatcggtg ctaaacagcg gctgcacgcg
     7021 ggaagcgaga tcggcaggca attcgccgtt cggccagatt tccgtcggta actgcgccag
     7081 gtcgagcgtc ggttcgctca acgccacgtt atattccggg cgctggtgta agccgcctgt
     7141 cactcttcct gcaactttca tggtcatctc atagcgaccg ttttcgccac gaatacgtaa
     7201 gcccatatcg tgcctacgca gccagttatc cggcgtttcg tagtaaatat tcagcaactg
     7261 cacggggtca tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg cctcaacggc
     7321 actgtgatta acaataaact ttaattcgat ttcctgagcc atggccttta cttatcggtt
     7381 atgtcacata agtgacgatg aacggcgaac ttaatgcgat cttttttgtc agtagatagt
     7441 attttgcgcc aaattgccat gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg
     7501 gcaagacctg acacagagga ttccgattga tgtcgaatgc tttgcgtagc gacactgatg
     7561 ccactactat cgttccactt tccatgacaa taacgacagc ctgatgccaa aattacgcct
     7621 gatcggatta actttactcg cacttagcgc gactgccgtc tcacacgctg aagaaacgcg
     7681 ctatgtttct gacgaactga atacctgggt ccgtagcggt ccgggagatc attatcgcct
     7741 cgtgggcacg gttaacgccg gcgaggaagt gaccttatta caaactgacg ccaacaccaa
     7801 ttatgcccag gtgaaagaca gctctggccg taccgcctgg atcccgttga aacaacttag
     7861 cactgagcca agcctgcgct cccgtgtgcc agatctggaa aatcaggtca aaaccctgac
     7921 cgataaactc accaatatcg ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa
     7981 agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa
     8041 cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc gtacagctgg atgacaaaca
     8101 gcgcaccatc atcatgcaat ggtttatgta tggcggtggc gtgctggggc ttggcttgct
     8161 gctcggtctg gtactgccgc acctgatccc aaaccgcaaa cgcaaagatc gctggatgaa
     8221 ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa ttgtgtattt gacgaaggga
     8281 tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg gatgcattgt tagggctatc
     8341 ggtcaaagac agagattggg tggtggtcgg cagtacgcca caggagatgc tcgacgcggg
     8401 ctaccagcag gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc atgaagagta
     8461 tgcgctggca cgtaccgaac ggaaatccgg ttccggttac accggtttta cttgctatgc
     8521 cgcaccggat gtcacgctgg aagatgatct taagcgtcgc gatctgacca ttaatgcgct
     8581 ggcccaggac gataacggcg agattatcga cccgtacaac ggtctgggcg atctgcaaaa
     8641 tcgtctgttg cgccatgttt cccccgcttt tggcgaagat ccgttacgcg tactgcgcgt
     8701 ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt attgccgatg aaactctgac
     8761 gctgatgcgc gagatgaccc atgcgggtga actggaacac ctgacgcctg aacgggtatg
     8821 gaaagagacg gaaagcgccc tcaccacccg caatccccag gtgttcttcc aggtactgcg
     8881 cgattgcggc gcacttcgcg ttttattccc ggaaattgac gcactgtttg gcgttccggc
     8941 acctgccaag tggcatccgg aaatcgacac gggtattcat accttaatga cgctctcaat
     9001 ggcggcgatg ctgagtccgc aggttgatgt ccgtttcgca actttatgcc acgatctcgg
     9061 taaaggactg acgccgccag aactttggcc gcgtcatcat gggcatggcc cggcgggtgt
     9121 taagttagtg gaacaactat gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc
     9181 cagactggtg gctgagtttc acgatctcat ccacaccttc ccaatgctga acccaaaaac
     9241 catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact
     9301 ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt tttgaatcag cggactaccc
     9361 gcaaggccgt tggttgcgcg aaggctggga agtggcgcag tcagtgccga caaaagcggt
     9421 cgttgaagcg ggatttaaag gcgtggagat tcgcgaggag ctgacccgac ggcggattgc
     9481 ggcggtagcc agctggaagg aacaacgttg cccaaagcct gactgagtat aaaactaaac
     9541 gttagcacga accgtcccct cgccccttcg gggagagggt tagggtgagg ggaacagacc
     9601 agcaccggtg cgaacaatca cccccacccc ggccctctcc ctacagagcg agggagaaaa
     9661 cccggcctgc tcccgaacct tttctcaatc aaaagaacac gacatacacc gcagccgcca
     9721 caataaagcg ataaatggcg aacgggataa acgaaatgcg cttaatcaat tgcaggaagg
     9781 ttttaatcgc tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga
     9841 tatcgccggt tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca
     9901 tcatcggtac cgccagcagg aacgagaact cggaagcagc gtaacggctc acgcccatca
     9961 gcatcccacc tgaaatggtc gccccggaac gggagaaacc cggccacagc gccagacact
    10021 ggaaacagcc aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg
    10081 gctctttcgg cttcaggcat tcggcggcaa tcagcaacaa accgccaacg accagcgcat
    10141 acatcacatt tatcgggtta aacaatgact taattgtgtc gtggaataat agccccagta
    10201 ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt taaacgacct ttgctttcac
    10261 cttcgtgctg caacgggcgg ccaaagtgga tgccaatcag gccaaacaga cgccgccaga
    10321 acatcactac taccgccaga attgatccta actggatcac aacttcaaag gttttcgccg
    10381 tgtcgccctc aaatcccaac aagtgaccga caataatcat atggcccgtg ctggataccg
    10441 gcagaaattc tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca
    10501 tatcgctcat caataaaccc ctaaattatt aaaatgtacc gcttgtccga actactgcag
    10561 tatgaccagg ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat
    10621 tattgccacg ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc
    10681 tgagtttgat acgcacccac ggcgagttga agcgtgctaa cagcagctcc gccacctctt
    10741 cagccacgcg ttccaccagc gcaaaacgcg ccccctcgac gtggctgacc accgtttctg
    10801 caatgtcagc gtaactgagg caatccgcca catcatcact tttcgccgct ttacggttat
    10861 cccatgccat ttcgatatcg aacactaact tctgttcgat ggtctgttcc cagtcgtaaa
    10921 caccaatagt ggtgattacc gaaagttgct ctataaatac aatatccatc acgtcctgcc
    10981 tgctttttgg ctaaccggat accacttccg gcgaaatgtg cgtattatcc acagattcat
    11041 cgttgaacac gaattttcaa aacggaacag cttatgagtg caatcgcgcc tggaatgatc
    11101 ctcatcgcgt acctctgcgg ctccatttcc agtgccattc tggtttgccg cttgtgtggg
    11161 ctgcccgatc cgcgaaccag cggctccggc aatccaggcg caaccaatgt gttacgtatc
    11221 ggtggcaagg gagcagccgt agcagtactg attttcgacg ttctgaaagg aatgttgcct
    11281 gtctggggcg cgtatgaatt aggtgtcagc cccttctggc taggcttaat tgccatcgcc
    11341 gcctgtcttg gacacatctg gcccgttttc ttcggtttta aaggaggaaa aggcgttgct
    11401 accgcttttg gagcaatcgc accaattggc tgggatctca ccggagtaat ggcgggaacc
    11461 tggttactga ccgtgctatt gagcggatac tcgtcgctgg gagcgattgt cagtgcactg
    11521 attgctccgt tttatgtctg gtggtttaag ccacaattca ccttcccggt ttcgatgctc
    11581 tcttgcctga tcctgctgcg tcatcatgac aacatccagc gtctgtggcg tcgtcaggag
    11641 acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg atttctggtg
    11701 gatctacatg acctgatagc cttcatcggg cttgcccagc cgttgctggc accatgccgc
    11761 cagaaattcc acgcagacac gaatttcatg ctgcgataga gcggctcccg gtaaacagcc
    11821 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    11881 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    11941 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    12001 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    12061 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    12121 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    12181 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    12241 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    12301 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    12361 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    12421 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    12481 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt
    12541 tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    12601 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    12661 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    12721 ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat ataaaaccac
    12781 atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    12841 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    12901 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    12961 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    13021 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    13081 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    13141 cgtggaagag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    13201 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    13261 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    13321 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    13381 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    13441 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attctgcgcc cgattggcag
    13501 ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag gactcaaccg
    13561 tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg catcgtcgcg
    13621 gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt
    13681 tatgaaaaag atcctgacaa cctcgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    13741 cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc accgccgttt
    13801 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    13861 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc cgacaaccag
    13921 tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa aactggtggt
    13981 tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca aggcgttaca
    14041 tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtagaag agattgaaga
    14101 agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt
    14161 cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa
    14221 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    14281 caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa cacgatgaaa
    14341 ccttccactg aatggtggcg atatcttgca ccgctggcgg tcatcgccat tattgctcta
    14401 cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt ttttactggc
    14461 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc
    14521 tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct cgcccagcca
    14581 ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc taattcggtt
    14641 atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggcttgga
    14701 cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt tctcggctat
    14761 gcggtgatgt tctccgagct gatcctagca cctgtaacac cgtccaactc ggcgcgtggt
    14821 gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    14881 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc
    14941 gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat tggactgatg
    15001 aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat gttgccgctc
    15061 agcattttac tgcttctgct ggttccctgg ctggcttacg tgctataccc gccggtactg
    15121 aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    15181 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc
    15241 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    15301 ctgcgcatta tcagttggga cgacattgtc agtaataaag cggcatggaa cgttttcttc
    15361 tggctggcct cgcttatcac cctcgcgacc ggactcaaca ataccggttt tattagctgg
    15421 tttggcaaac tgttagcagg cagcttaagt ggttatccgc cgacgatggt gatggtgacg
    15481 ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc gtatacctcc
    15541 gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    15601 ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    15661 ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta ctggcgactg
    15721 ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt actgtggatg
    15781 cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca cccaatagtt
    15841 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc cgagatccgc
    15901 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt
    15961 acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca tttcagccag
    16021 cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc gcttaaagcc
    16081 cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa
    16141 ggcgcgggcg atatcagcac gcgtctggtc gtcggtgctg ttgtcacgaa tcgtgttcgc
    16201 cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    16261 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta acggtccgcc
    16321 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc
    16381 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    16441 accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt ccagcatcgg
    16501 cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc aggcaaacgc
    16561 cagagagcgc cccacggttg cgccaaccag cagtgcgccg actaatccag ggcctgcggt
    16621 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat
    16681 caacggtacg gttttacgca catgatcgcg ggaggccagt tcaggcacga cgccgccgta
    16741 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    16801 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    16861 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    16921 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    16981 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    17041 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    17101 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    17161 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    17221 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    17281 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    17341 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    17401 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    17461 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    17521 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    17581 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    17641 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    17701 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa
    17761 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    17821 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc
    17881 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    17941 atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac
    18001 gcagttacga tcgtttccgc gaacgggtga tattccccat tcgcgataaa cgcggtcggg
    18061 tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg
    18121 aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    18181 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    18241 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    18301 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    18361 gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc
    18421 tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta
    18481 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    18541 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    18601 cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    18661 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    18721 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    18781 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    18841 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    18901 agccaggtct gacctccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    18961 cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct
    19021 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    19081 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    19141 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    19201 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    19261 ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc
    19321 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    19381 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    19441 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    19501 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    19561 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    19621 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    19681 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    19741 ccaagttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    19801 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    19861 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    19921 agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga
    19981 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta
    20041 cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga
    20101 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    20161 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    20221 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    20281 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    20341 aaaactgcac gatgtctctg aagaagtgca tcgcgccctg caaaaactgc agcagattga
    20401 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    20461 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat
    20521 ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    20581 taacatcggc ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    20641 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    20701 caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg
    20761 ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct
    20821 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    20881 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    20941 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    21001 ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg cgtttcggta tcgatatgaa
    21061 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    21121 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg
    21181 tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag
    21241 tctctctgca caaacgccac cttt
//
LOCUS       DADLZM010000063        21254 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLZM010000063 DADLZM010000000
VERSION     DADLZM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.063"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(32..1012)
                     /locus_tag="JNY73_002235"
     CDS             complement(32..1012)
                     /locus_tag="JNY73_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY8048239.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSALDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="JNY73_002236"
     CDS             complement(1020..1730)
                     /locus_tag="JNY73_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY8048240.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNY73_002237"
     CDS             complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNY73_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY8048241.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4143..5552
                     /gene="glnA"
                     /locus_tag="JNY73_002238"
     CDS             4143..5552
                     /gene="glnA"
                     /locus_tag="JNY73_002238"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY8048242.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5725..6774
                     /gene="glnL"
                     /locus_tag="JNY73_002239"
     CDS             5725..6774
                     /gene="glnL"
                     /locus_tag="JNY73_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY8048243.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6786..8195
                     /gene="glnG"
                     /locus_tag="JNY73_002240"
     CDS             6786..8195
                     /gene="glnG"
                     /locus_tag="JNY73_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY8048244.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8307..8417
                     /gene="yshB"
                     /locus_tag="JNY73_002241"
     CDS             8307..8417
                     /gene="yshB"
                     /locus_tag="JNY73_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY8048245.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNY73_002242"
     CDS             complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNY73_002242"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY8048246.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNY73_002243"
     CDS             complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNY73_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY8048247.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11299..11931
                     /locus_tag="JNY73_002244"
     CDS             11299..11931
                     /locus_tag="JNY73_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY8048248.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNY73_002245"
     CDS             complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNY73_002245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY8048249.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15101..15343
                     /locus_tag="JNY73_002246"
     CDS             15101..15343
                     /locus_tag="JNY73_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048250.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFIFHAVKNKSIPNN"
     gene            15463..16395
                     /locus_tag="JNY73_002247"
     CDS             15463..16395
                     /locus_tag="JNY73_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8048251.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16436..17866)
                     /locus_tag="JNY73_002248"
     CDS             complement(16436..17866)
                     /locus_tag="JNY73_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY8048252.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRIQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNVVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNY73_002249"
     CDS             complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNY73_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY8048253.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNY73_002250"
     CDS             complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNY73_002250"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY8048254.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQESPLQLTPMY"
     gene            complement(19727..19996)
                     /locus_tag="JNY73_002251"
     CDS             complement(19727..19996)
                     /locus_tag="JNY73_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY8048255.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20066..20650
                     /gene="mobA"
                     /locus_tag="JNY73_002252"
     CDS             20066..20650
                     /gene="mobA"
                     /locus_tag="JNY73_002252"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY8048256.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20632..21159
                     /gene="mobB"
                     /locus_tag="JNY73_002253"
     CDS             20632..21159
                     /gene="mobB"
                     /locus_tag="JNY73_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY8048257.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5181 a         5351 c         5122 g         5600 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctaaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc catcgctggc
     1921 aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga
     1981 cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt cacttctacc
     2041 agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc
     2101 agaacaacag cttcgtcagt accggaagca cgcatgttgg tcagtttctt accggtcagg
     2161 cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc ttcgtaaact
     2221 tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg
     2281 acagctttac cctgaccgtt agagatcagt acaccgttct gacgctgacc cacttcgccc
     2341 ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg
     2401 gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt
     2461 acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc
     2521 tgcatcacag aaccctgatg ctgttcttca acgtccagcg tcacgttttc atacggctct
     2581 tgtttacgac cgtcgatttc acggaagatt actttcggac gggataccgc cagttcgaaa
     2641 ccttcacgac gcatgttttc gataagaaca gagaggtgca gttcaccacg accagaaacg
     2701 cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct
     2761 ttgttcagac gatcgaggat ctgacgagac gttacgaact taccttcttt accgcagaac
     2821 ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc
     2881 agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc
     2941 gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc cagaccgagg
     3001 tggcccagca ctttaccgac tttcgcgtta cgggttttgc cttcgctatc gatgatagta
     3061 acctgctggt tcggcttcac tttaccacgc ttgatgcggc caatgccgat aacgccaaca
     3121 tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc
     3181 ggcgcaggaa cgtggtcaac aatcgcctga tacagcgggg tcatgtcttc cgccatatct
     3241 tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc
     3301 tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca
     3361 ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca
     3421 aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc
     3481 agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc
     3541 cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg
     3601 aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt
     3661 tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccaa ggtggtttta
     3721 ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct
     3781 caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac
     3841 aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa
     3901 aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat tgaagcacta
     3961 tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag catgataacg
     4021 ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac
     4081 ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag gagagttaaa
     4141 gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt
     4201 tgcgcttcac cgataccaaa ggtaaagaac aacacgtcac tatccctgct catcaggtga
     4261 atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag
     4321 gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt
     4381 tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag
     4441 gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg cgttccactg
     4501 gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc
     4561 gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga
     4621 actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt
     4681 acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg tgtctggtga
     4741 tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga
     4801 acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca
     4861 aatatgtcgt gcataacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac
     4921 cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg
     4981 ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg
     5041 gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt
     5101 ataagcgtct ggtcccgggc tacgaagcac cggtaatgct ggcttactct gcgcgtaacc
     5161 gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac
     5221 gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc
     5281 ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc
     5341 tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga
     5401 acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg gatgaagcga
     5461 tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc
     5521 cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaca
     5581 acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc ttttttctcc
     5641 gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca ctaaaatggt
     5701 gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc tgggcagatc
     5761 ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat ccattacgcc
     5821 aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg tacgccgtta
     5881 ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag tctggaggcg
     5941 gggcaaggct ttaccgataa cgaagtgacg ctggtcatcg atggtcgctc gcatatcctt
     6001 tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc accgatggat
     6061 aaccagcgcc gcttaagcca ggaacaacta caacatgctc aacaagtagc tgcccgtgat
     6121 ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gcggtttacg tggcgcggcg
     6181 cagttactca gcaaagcgtt acctgaccca tcactactcg aatataccaa agtgattatc
     6241 gaacaggcgg accggctgcg aaatttggtc gaccgtctgt tggggccgca gctgcccggt
     6301 acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg
     6361 gaactgccgg acaacgtgcg gttgattcgt gattacgatc ccagcctgcc ggaactggcg
     6421 cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg
     6481 ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca actgacctta
     6541 cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg gccgggcatt
     6601 ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga aggtggcacc
     6661 gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa aattgaattt
     6721 accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa ataaaggtga
     6781 cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc cgttgggtgc
     6841 ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac ggcgcggaag
     6901 tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg
     6961 gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg cttccggtca
     7021 tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa caaggggcgt
     7081 ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta
     7141 tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc ccaacgaccg
     7201 atatcatcgg cgaagcgcaa gccatgcagg acgtgttccg tattatcggt cggctttcgc
     7261 gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa ctggtcgctc
     7321 atgccctgca tcgccacagt ccgcgcacca aagcgccgtt tatcgcgctg aatatggcag
     7381 ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc gcgtttactg
     7441 gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggcacg ttatttctcg
     7501 acgaaattgg agatatgccg ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg
     7561 gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg
     7621 ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc
     7681 accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc
     7741 cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt
     7801 tgctgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc
     7861 agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa gtgttgattc
     7921 aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg caaatgcaac
     7981 cggacagttg ggcgacactt ttagcgcagt gggcagacag agcgctgcgt tccggtcatc
     8041 aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg accgcgttgc
     8101 gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc cgcaacactc
     8161 tgacgcgtaa gttaaaagag ctggggatgg agtgattcac ggcttgtgtg taaagattga
     8221 ttattgagcg caaattgctg gtattttgcg ctttactgtt ccgataagtt cagtatgatc
     8281 ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg gtatcgagtg
     8341 gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt gccgcgatga
     8401 ttgggctgtt tagctgaagc tcgttgttta tgccggatgc ggagaaagtc gtaggcctga
     8461 taagacgcga ccagcgtcgc atcaggcatc atgctcagat gcctgatgcg gcgtaaacgc
     8521 cttatccggc ctacaaaagc aataatttca acaggttttg ttgcccagcg catcaggcaa
     8581 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
     8641 actctttaaa tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca
     8701 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
     8761 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
     8821 aagtaatcag cgaagtgcaa atcccactgt ttctcaagag gggcgtaatc cagacggaag
     8881 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
     8941 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
     9001 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
     9061 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
     9121 gccaccgcca gctcgtcatc aggacgggca aagtgatcca taccgataaa ctgatagccc
     9181 gattgcgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
     9241 aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta
     9301 aaaacgctca gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc
     9361 tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc
     9421 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
     9481 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg
     9541 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc
     9601 gcatcggtat tgaaatggaa gttttcgcgc agcagcttca tcagacggct gatttgcgct
     9661 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
     9721 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
     9781 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
     9841 gggatatgta cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac
     9901 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
     9961 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    10021 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg
    10081 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    10141 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    10201 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    10261 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    10321 gtttagcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    10381 ccagcagcgc atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa
    10441 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc actcccaatg
    10501 gaataggggt tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg
    10561 acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    10621 gcttacggtc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    10681 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    10741 tattccgtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    10801 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    10861 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccgt
    10921 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    10981 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    11041 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    11101 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    11161 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    11221 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    11281 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    11341 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    11401 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    11461 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    11521 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    11581 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    11641 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    11701 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    11761 cgtaaagcgc tattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    11821 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtagaca agctgcggca gaaactggat
    11881 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    11941 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    12001 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    12061 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    12121 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    12181 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    12241 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    12301 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    12361 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    12421 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    12481 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    12541 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    12601 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    12661 gcccagctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    12721 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    12781 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    12841 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    12901 gatatctttt ccttccgcga atgcggtcag caagccttta tcacgcgaga gatgcgccat
    12961 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    13021 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    13081 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    13141 cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgact tcagcttcgc
    13201 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    13261 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg
    13321 tttttcaaac agaatggttt gcaactgctt ggtggaagaa aggttaaatt cttcacctgc
    13381 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagaatgatt
    13441 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    13501 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    13561 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    13621 ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg caatctcttc
    13681 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    13741 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc
    13801 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    13861 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgtgag agatttgatc
    13921 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    13981 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    14041 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    14101 gatagtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    14161 aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    14221 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    14281 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacatcggt
    14341 tttaatcgtc gccaactggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    14401 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    14461 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    14521 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaagtcga tgatcaattc
    14581 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    14641 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctaat
    14701 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    14761 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg
    14821 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    14881 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    14941 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    15001 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    15061 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    15121 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    15181 gaaaatcttt tgtagatctg ctggatcgct cgcaaatcgt catgtggata actttgtgca
    15241 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    15301 tatttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg ggattttttc
    15361 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcattt
    15421 tctgctaaaa tctgcgcctt gcgcttcttt agtgcactgt ctatggctaa ccttttgaat
    15481 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    15541 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    15601 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    15661 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    15721 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    15781 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    15841 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    15901 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtcaa
    15961 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    16021 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
    16081 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    16141 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
    16201 ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt acagcctatt
    16261 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    16321 tggctgaact cattgtggca ggaaaaagac cggcttctga cgtctctcat atcatcacag
    16381 cgtcagaata agtaagtttt actcgcctct gaatgagcag aggcgagtga gtattttaat
    16441 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    16501 caactttacc cggtgtataa cgcagctgca gcgaggcggt attgttgtca atcgtgatta
    16561 actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg
    16621 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    16681 taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg gatgatgaag
    16741 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    16801 tccatcttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
    16861 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
    16921 cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc actgctgagg
    16981 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    17041 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    17101 ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    17161 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    17221 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    17281 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
    17341 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    17401 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    17461 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactacgttc acttttgccg
    17521 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    17581 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    17641 tatcgaatgt catttgcatt tgaatacggg aattaaaaaa gcctttgtca aagtttttat
    17701 aacttaacgt catatcgtag gcatgttcac ctttcttttt cgcatcgtta aaatctttaa
    17761 taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    17821 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    17881 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc ggcaggatgc
    17941 attatcagaa tgtatttata tttattaaat aataaaaaaa gcccgtgaat attcacgggc
    18001 tttatgtaat ttacattgaa ttattttttc tcggacagat atttcaccgt atcagcatac
    18061 tgctgaacaa aaacatccat attgctggta tccataccct gcggattcag ctgatattta
    18121 ccgttaacaa acatcgccgg aacaccacgc aattgcacat cagctgcggc tttttcctgc
    18181 tgagcgacca gagatttcac cacgaagctg ttccacgccg cgtcgtactc ttcaccttta
    18241 atacctgcat tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc
    18301 tgtacgcctt caaacagcgg aacagtcact ttgtcttcca cgcccagcgc catcgccaca
    18361 gcccatgcct gagtcagatc tttgcccagg tcaccaccca tgaagttgac gtggtattta
    18421 gtcatcttca cgccttccgg cagttttttc ttcacattat cagaaatatg cagaacttct
    18481 tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca
    18541 gctaccggtt tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgca
    18601 ctaaacgcta aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc
    18661 cgattaatac ataggtgtta attgcaaagg ggattcttgt agaacttttg cctgttctat
    18721 aaaagtcgcc gtctgtcgta gccagtaatc ttccccggtt aaccacggga aatttttcgg
    18781 gaacgcggga tcagcccaat gccgcattag ccaggcaaga taataaacca aacgcatggc
    18841 gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc
    18901 ttcaataata gtttccagtt gcatccgctg ctgggcttta tcgccattga gcaacatcca
    18961 caaatcctga atagctggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc
    19021 gcgccagaga atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc
    19081 ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc gctttcagaa atgccgcttt
    19141 caacccggaa ggtatcagtg tagcgtcctc aaacagcttg cgaggctcaa tgagatattc
    19201 attcagaccg atggtcgggc gatgaataaa aagctgtttg cgccccgtct ggtgcatacg
    19261 ccctaaataa cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc
    19321 accgacgctt ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt
    19381 aaaggccaca ggtgctgcga ccggaacttc atcatttacc agctgcaacg caaattgatg
    19441 ttcttcgagg atttgatcgg ctgtccaacg ttcagggcga taaaatttga cgacaaaacg
    19501 tcgacgctct tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag
    19561 accggaatcc acccggatcc cttgctcaaa cagagcgtcc atgatggtat ccgggtgtag
    19621 tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg
    19681 aaggatatca tttcacgatg attttggtgg cgccgcttac aagcttttac tctttaatta
    19741 cgccgcgagc gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg
    19801 ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg
    19861 tcaaatccgc cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca
    19921 acaagttaaa gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca
    19981 gacgtttaca tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa
    20041 tcccacaata aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct
    20101 ggcaggcggt aaagccagac gaatgggcgg cgtagataaa ggattgctgg acttaaatgg
    20161 caaaccgctg tggcaacatg tcgctgacgc gcttaagacg cagctctctc acgtcgtggt
    20221 taatgctaat cgtcatcagg aaatctatca ggcaggcggt ctgaaagtga ttgaagattc
    20281 actggcggat tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagctgg
    20341 tgagtggttt ttgttttgtc cgtgcgatac gccttacatt ccccatgatt tagccgcccg
    20401 gcttaatcat cagcgcaaag atgcgcctgt cgtgtgggtc catgacggtg aacgcgatca
    20461 cccgactatt gctctggtaa accgcgctat tgagccttta ttactggaat atctgcaagc
    20521 aggagaacgc cgggtaatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag
    20581 cgatcataaa gatgcatttg ttaacgtgaa tacgccagag gagcttgccc gatggcagga
    20641 aaaacgatga taccgttact cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt
    20701 ttgaaaaaac tgatcccggc attatgcgcc agagggatcc gtccggggct gattaagcat
    20761 acgcaccatg atatggatgt tgataagcca ggcaaagata gctatgagct gcgcaaggct
    20821 ggcgcggcac aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca
    20881 gacgaagaag agctggatct acattttctc gcaagtcgaa tggatacctc aaagctggat
    20941 ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat
    21001 gcagccggac atcgacctga agaattagtg atagacaggc atgttattgc tgtagccagt
    21061 gatgtgccgc ttaatcttga tgtcgcatta ctggatatta atgatgttga ggggctggct
    21121 gattttgtgg tggagtggat gcaaaagcag aacggataag caagagtagg ctggatcaga
    21181 cgcagccagt gtcgcatccg acctttttct gcaccagcgc aaaaaggccc gactgggcct
    21241 tctgcttaat ttga
//
LOCUS       DADLZM010000064        21001 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLZM010000064 DADLZM010000000
VERSION     DADLZM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.064"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(703..3273)
                     /locus_tag="JNY73_002254"
                     /pseudo
     CDS             complement(703..3273)
                     /locus_tag="JNY73_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189288.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3391..4584
                     /locus_tag="JNY73_002255"
     CDS             3391..4584
                     /locus_tag="JNY73_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY8048258.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKTTQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4782..6035)
                     /locus_tag="JNY73_002256"
     CDS             complement(4782..6035)
                     /locus_tag="JNY73_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY8048259.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6362..7552
                     /gene="hmpA"
                     /locus_tag="JNY73_002257"
     CDS             6362..7552
                     /gene="hmpA"
                     /locus_tag="JNY73_002257"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY8048260.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7597..7935)
                     /gene="glnB"
                     /locus_tag="JNY73_002258"
     CDS             complement(7597..7935)
                     /gene="glnB"
                     /locus_tag="JNY73_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY8048261.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7996..9330)
                     /gene="glrR"
                     /locus_tag="JNY73_002259"
     CDS             complement(7996..9330)
                     /gene="glrR"
                     /locus_tag="JNY73_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY8048262.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9320..10033)
                     /gene="qseG"
                     /locus_tag="JNY73_002260"
     CDS             complement(9320..10033)
                     /gene="qseG"
                     /locus_tag="JNY73_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY8048263.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10198..11625)
                     /gene="qseE"
                     /locus_tag="JNY73_002261"
     CDS             complement(10198..11625)
                     /gene="qseE"
                     /locus_tag="JNY73_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY8048264.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12183..16070)
                     /gene="purL"
                     /locus_tag="JNY73_002262"
                     /gene_synonym="purI"
     CDS             complement(12183..16070)
                     /gene="purL"
                     /locus_tag="JNY73_002262"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY8048265.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16328..17884
                     /gene="mltF"
                     /locus_tag="JNY73_002263"
     CDS             16328..17884
                     /gene="mltF"
                     /locus_tag="JNY73_002263"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY8048266.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17881..18417)
                     /gene="tadA"
                     /locus_tag="JNY73_002264"
     CDS             complement(17881..18417)
                     /gene="tadA"
                     /locus_tag="JNY73_002264"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY8048267.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18442..19077)
                     /gene="yfhb"
                     /locus_tag="JNY73_002265"
     CDS             complement(18442..19077)
                     /gene="yfhb"
                     /locus_tag="JNY73_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY8048268.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19286..20134
                     /locus_tag="JNY73_002266"
     CDS             19286..20134
                     /locus_tag="JNY73_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY8048269.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVVQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20473..20964)
                     /locus_tag="JNY73_002267"
                     /pseudo
     CDS             complement(20473..20964)
                     /locus_tag="JNY73_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436854.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         5041 a         5674 c         5347 g         4939 t
ORIGIN      
        1 gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc aggccggatt
       61 aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga gtcccgcgga
      121 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
      181 cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
      241 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
      301 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
      361 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
      421 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
      481 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
      541 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
      601 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
      661 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
      721 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
      781 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
      841 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttt
      901 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
      961 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
     1021 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
     1081 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
     1141 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
     1201 gtccagtgcg ttcagcatct ctgccgccaa ggtgggcatt ccccagttaa tcagttgccc
     1261 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
     1321 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
     1381 acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
     1441 cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
     1501 aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca gaccataata
     1561 gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
     1621 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
     1681 gtttttgttc agcgcatgtg cgcgtttcag cgcctgactg gcatacgcca ccgcttgcgg
     1741 gaaatctgcg cggttatatt ccagcatcgc cagcgccagg ttacagcgat aatccagcgg
     1801 gtccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt gatgatattg
     1861 ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt gtgctgccag
     1921 tggcgctttg gcgacgtccg gcaacggcaa ctcctgcggc tgatgttcct gataactcag
     1981 tacaatattg ccgtcggcat cggagagttc aatggtcagt ctgtcaggat tgataccgtg
     2041 caacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga gcaacgcgtt
     2101 gcatttgccg atttcgcgga tcgccagacg gtatccgttc aacggagaga tggcatacag
     2161 cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc gggaggcatt
     2221 ttgcaccatg cacagagaat gataaggcag gaaatactgc tcgaaacgct tctcttcgta
     2281 agcatcaagc caggtaaaat caggctggtt atcggcaaaa ataccggtca tcagttcgat
     2341 atacgggccg ttattgtcgg tcagactctt atcccacgcc tggccaaatt cactgtgtcc
     2401 ccaactccac tgttttttac ctggcgcaat atggtggttg gcaacgtgca gcaaaccgcc
     2461 atcttcatcg tgacaccacg cgccgacaaa atcgtactgc gatttttcgg ccatatatga
     2521 ggttggaaca ggcacatttt tatagcgaga aatgtccact ccggcggagt agtccacttt
     2581 gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt gatcaaacac
     2641 cgcagtcaca tccggtggga aaacgctctg atgcccttca ccccctttca ctgccgggtt
     2701 ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc tggcgatttc
     2761 cagtgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac catgcatcgg
     2821 ctccgtttcg ccgacccaca ccgtctgtgc accgtcttca tgggcttcga gggtgaaatc
     2881 aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa tcccgccgga
     2941 gatccacggt cccagcagcc ccaccagcgc aggtttaatg acttcattgt gatagacaaa
     3001 atcgcgctgt ttcactttat cccatgcgcg atgcacccga ccgcccagtt ccggcaggat
     3061 catcactttg atgtagtcgt tttccagcca caccgcctgc taggatttca gggttttctg
     3121 ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc cctgataaac
     3181 gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg tcgggatctc
     3241 aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa caagacatac
     3301 tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg cggaataatc
     3361 acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa tcaacagatt
     3421 cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg cgccaataaa
     3481 tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat tttgcaggaa
     3541 ctggaaagcg aaaaacgggt ggtgaatatt gacgatgaaa gccagacgcg cgggcatagt
     3601 agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgcctgaa cgtgacgccc
     3661 accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggcga atttgagtat
     3721 ttacagattg atgtaccgac gccgcaggcg ctgctgtccg aaatcgaaaa atgctggcat
     3781 cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat ccacgggcag
     3841 gttgatccgg tgaccggcgt atcgcaaacc atgccgcaag cgccgtggac aacgccagtt
     3901 gaggtgaagt atctgctgga agagaagctg ggcattcggg tgctggtcga taatgactgc
     3961 gtgatgctgg cgcaggcgga gaaatggcaa aataattcgc aggaacggga tttctgcgtg
     4021 atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat ttatcgcggc
     4081 agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg cgtcgtctgc
     4141 gactgtggac gttacggctg cctggaaacc gtcgcctcgt taagtgcatt aaaaaaacag
     4201 gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga aaaactgaca
     4261 accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggatcaccag ctgggttgat
     4321 cgctctgcca atgccattgg tttgagtctg tataacttcc tcaacatcct caatattaat
     4381 cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa tactattatt
     4441 cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa aacgacgcaa
     4501 attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta tttgtatgtt
     4561 gaggcgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc cgaaggaagc
     4621 cttgatgatc ataaacgaaa aattgcctga tgcgctacgc ttatcaggcc tacacggaga
     4681 ttgcaatata ttgaatttac aagattttgt aggcctgaga aggtgttcat gccgcatcca
     4741 acatgaacaa cgagcacatt gtcagcaaat caccgtttcg cttatgcgta aaccgggtaa
     4801 cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga taacggcttc atcattgatg
     4861 ctgtccagca cgtcacacat ccagccagcc agttctttcg cttcagcttc tttaaagccg
     4921 cgacgggtaa tcgccggagt acctacacga ataccggagg tcacaaacgg gctcttcgga
     4981 tcgttcggta cgctgttttt gttgacggtg atgttagcac ggcccagagc ggcgtctgct
     5041 tctttaccgg tcaggttttt atcaaccaga tcaaccagga acaggtggtt atcagtgccg
     5101 ccggaaacca ctttgtagcc gcgctcgagg aacacttcta ccatcgcttt agcgtttttc
     5161 gcgacctgct gctggtaagt tttgaactca ggctccatcg cttctttcag agcaaccgct
     5221 ttaccggcga ttacgtgcat caacggaccg ccctgaccac cagggaaaac ggcagagttc
     5281 agttttttgt acagctcttc gctaccacct ttcgccagga tcaggccgcc gcgcggaccc
     5341 gccagggttt tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag
     5401 acgccagcag caaccaggcc cgcaacgtgc gccatatcaa cgaacagata agcaccgatg
     5461 ctgtcagcga tttcacgcat tttcgcccag tcaaccacgc cggaatatgc agagaaacca
     5521 ccgataatca ttttcggctt gtgttctttg gcttgttttt ccagatcggc gtaatcgata
     5581 tgaccggtag catcgatacc gtaaggaacg atgttgtaca gtttaccgga gaagttaacc
     5641 ggagaaccgt gagtcaggtg accgccatgc gccaggttca tacccagaac ggtatcacct
     5701 ggttccagca gcgcggtgta gaccgcaaag ttagcctggg agccggagtg cggctggacg
     5761 ttagcgtagt cagcgccgaa cagttctttc gcacgatcga tcgccagttg ttcaacgata
     5821 tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc atatttgttg
     5881 gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc
     5941 agttcgatgt gctcttcctg acgtactttt tcctgctcca tagcctgcca cagttcggca
     6001 tcataatcgg caatgttcat ttcacgcttt aacatccgca tctcctgact cagctaacaa
     6061 taaaattttt ggcctttata ggcggtcctg ttggacaacg gcgaacagta taaccgaatc
     6121 attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa cgattacctt aaggctacgc
     6181 aaggctttgg agaataaaga gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg
     6241 caaatttttc acttcatcac attctttctg aaaaacacca aagaaccatt tacattgcag
     6301 ggctattttt tataagatgc atttgagata catcaattaa gatgcaaaaa aggaagacca
     6361 tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac
     6421 ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa
     6481 agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc
     6541 tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa
     6601 aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca tcgtcggtga
     6661 acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg
     6721 gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg aaatctataa
     6781 cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca ttgtggctaa
     6841 aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt
     6901 ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca
     6961 tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtatcgc
     7021 ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg ccaatgttgg
     7081 cgatgtcgtg aaactggtcg ctccagcagg tgatttcttt atggctgtcg cagatgacac
     7141 accagtgacg ttaatctcta ccggtgttgg tcaaacgcca atgctggcaa tgctcgacac
     7201 gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga
     7261 agttcacgcc tttgctgatg aagttaagga actggggcag tcactgccgc gctttaccgc
     7321 gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt ttgatagcga
     7381 aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta
     7441 tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg atctgggcgt
     7501 gaagcaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt aatttgatgt
     7561 tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt cgtcctcttc
     7621 accggtacgg atgcgaatga cccgagccac gtcaaagacg aagattttac cgtcaccgat
     7681 tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa tgtcgtccgg
     7741 tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg cgccgcggta
     7801 cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg tcatgccggt
     7861 aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt ttataatcgc
     7921 atcaatcttt ttcatgctat tccttgaaaa ggttgcctgt cttttgatct gctaaacgta
     7981 acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc gggaaagcag
     8041 tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga cgttgccttt
     8101 ggtgatttgc agcagcttac gcaaatagtt gagttcaaaa tgattacgtg cctcaacaaa
     8161 ggttggcagc gccgtatttt caccctccag cgcctgctcc accagcgcat cactaatcac
     8221 cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc gcacattacc
     8281 cggccagctc gcggtcatca ggcgtttcat cgcatcggta gagaacgcgc ggacaaacgg
     8341 tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa tgtcttctgt
     8401 gcgctccgcc agtgccggaa ttttcaggct gacaacgttg aggcggtaat agagatcttc
     8461 gcggaattcc ccgcgcgcca tcgcttttga cagatcacgg tgagtggcag aaataatccg
     8521 cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct cctgcaacac
     8581 gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga gaaatagcgt
     8641 tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag taaacgcgcc
     8701 acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc cacagttaat
     8761 agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg cgaaaatctc
     8821 tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg ccaccagccg
     8881 cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct cgcgccagcg
     8941 ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat atagcgcatc
     9001 tttatcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag caacggcatc
     9061 ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca ctttctggat
     9121 ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga taactaaatc
     9181 tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg tgaccacact
     9241 gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat cgtcatcgac
     9301 caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg gatggcgcgg
     9361 gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg cgggtcgaga
     9421 gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc agttgctgct
     9481 gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac tgctggagtt
     9541 tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg tcgcgccaga
     9601 gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca atacgcgcga
     9661 ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc ccttgtttaa
     9721 aggtattttg ccagctgccg tcgtcatatt ggcgagcttg ctgacgcgac tgcgcaggca
     9781 ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt tcggttgatt
     9841 taccttgcag cgcccagatg tcactacatt cagtagaaag atagtcagcc agttgataaa
     9901 ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc tgcacgcaac
     9961 ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat aatcgttgaa
    10021 aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat tgttgcagac
    10081 agtctggaca tggacagcgc ggagaaaccg gggcgtacat atcgtacgtg aagatttcga
    10141 gcactacccg gggccgaact gacaaataaa atagcctgat aggataggct gttagcatta
    10201 tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc cgctctcgtc
    10261 gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc ttaatcccag
    10321 accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg gttcgaagat
    10381 catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa tataaacccg
    10441 cgcaccatgt aaactgctgc gaaggcaaat gttaccggat tcagccccgt agtgcaccgc
    10501 attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca ggcaagctgt
    10561 tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc tatgagcaga
    10621 aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg cactgtccgc
    10681 ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc agctgctgct
    10741 atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa cctgatcagc
    10801 cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat gagataaatg
    10861 tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta accaaagaat
    10921 acgttgccca accgagcgta actcgctcgg tccacagaac gaaacgctat tgcccagaga
    10981 acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc cgataatcat
    11041 ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg attgccaacc
    11101 aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag agaacaccac
    11161 tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa gacgcgcggc
    11221 ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt tcaaatcctg
    11281 acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat cgagcatttc
    11341 gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt ccagcacgca
    11401 atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt cactacgccg
    11461 ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg cattcaggct
    11521 ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa atgccagcat
    11581 taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt tactctcgtc
    11641 agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag ccgggaatta
    11701 cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga tggttgatat
    11761 agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat tcgaatttta
    11821 tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct tactattatg
    11881 cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg ttgctttgag
    11941 agatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct aaaaagagac
    12001 aacttaagat aaacttatta gattacaata aaatcaatca tttaagtgtc gccagaaagc
    12061 gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc ggagttggga
    12121 gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa tgaactgatg
    12181 acttacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc atcctcgccc
    12241 cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg gtgcggcatc
    12301 ataatggtga ctcgaccact ttcagtcgta accgccgtaa taccgttcgg tgaaccgttc
    12361 gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg cagtgccacc
    12421 agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac gcgcccttca
    12481 ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacag cagagacggg
    12541 ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt gttacgcaca
    12601 aaacgtggcc acaactcgct ccccgggatc agttcacgca ggttagacat catctggcaa
    12661 ccgttacata cccccagggc cagcgtttgc ggacgatgga agaaggttgc aaactcatcg
    12721 cgtacacggt cattgaacag gatagacttc gcccaacctt caccggcacc cagcacatca
    12781 ccgtaggaga aacctccgca cgcgaccagg gcgtgaaaat cctccagtcc cgtacgtcca
    12841 gtcagcaggt cgctcatatg cacgtcgata gcatcaaagc ccccacgttg gaaagctgcc
    12901 gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt cggacgtgcg
    12961 ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag ttttacattc
    13021 aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca ctccgggttg
    13081 tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa cgtagtgcgg
    13141 ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc ggaaaccgcc
    13201 tgcccaacat aatggacgca atccgcaagc ccatgctgtg ccagtacgga ctcgaccgct
    13261 tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa caacgccgcc
    13321 aggcgatcgt cacccagaga ggcgatatcc gcgtcaatgc cacaatgacc tgcaaaggcc
    13381 atttccgcca gcgttaccag caggccgcca tcagagcggt cgtgatacgc cagcagctta
    13441 cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttggac aacatcgcgt
    13501 acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt tgcgcccagc
    13561 gcgttattgc ctttgcccaa atcaatcagc agcagtgcgt tatcttcggt acaaagctgc
    13621 ggcgtgatgg tgtgacgtac atcttccacg cgggcaaatg cagaaatcac cagcgacagc
    13681 ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat cgacatagag
    13741 tctttaccca ccgggatcgt caggcccagc gccggacaaa gttcttcacc cacggcttta
    13801 acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat ccagttggcg
    13861 gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt taacgcttca
    13921 ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc acgctcgcca
    13981 atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc gcagttagcg
    14041 accggcacct gccacggccc caccatctga tcgcgcgcca ccatgccggt tacgctgcgg
    14101 tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag tacgcgtttc
    14161 accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc tttcagcgtt
    14221 tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg cagatcgatc
    14281 ggctgattat caaaatgacg atcgtgcaga gaaagatgca gttcttcggt cgcttcacca
    14341 atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg taattgatcg
    14401 gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc cagcgggctc
    14461 atgcccggtt cgtcgcttag aatatcgcgc agttcaaatt taccgccgcg tccgccgtcg
    14521 ctcaccagct ccggcatcgc gttagaaaga ccgcccgcac cgacgtcgtg gataaacaga
    14581 attgggttag catcaccaag ctgccagcaa cggtcgatca cttcctggca acgacgctcc
    14641 atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga ctgaccagac
    14701 gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc gccgagaacg
    14761 accagcttcg caccgacgtt gatctcgcct ttttgtacgt gatcggcgcg aatgttgccg
    14821 atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc attgtggctg
    14881 ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc aaattcgttg
    14941 ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt aacaatgcgc
    15001 tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caggaattcg caggttggat
    15061 acggagaaac caaccaaacc agctttcggc ttcgcgccgc gcccggtggc accttcatcg
    15121 cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc cgtcgggtgg
    15181 ttgtgagttt cgactttcat caggatgtgc gccggttcct gatggaaatt gtagcggccc
    15241 gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc ggcgttatct
    15301 ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat catcctgaac
    15361 agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat tttgtggcgg
    15421 cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg gttacgacca
    15481 agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc caggccaaga
    15541 cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac gcttgtgacc
    15601 ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa aaagaccgtt
    15661 tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt ggtcagcgta
    15721 ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg tagcccgcag
    15781 ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg acgcggggtc
    15841 accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata tttcagcagg
    15901 cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc aaaatggaca
    15961 tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc cagcagtttg
    16021 ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat aagtctctcg
    16081 tcttctaagc tttcggtgta cccaaggggg ggaaacgggc gtcattataa agaatctgat
    16141 gcgctgacga aaccgtttgc gtggaaataa aatcaccatc gtgaattagc aacgcgtgcc
    16201 gccaatggct gtaataagtt gccatctggc gcaggtttac gcaaaatgcc gctcatttat
    16261 gagtaaacct ttcactatta ttacgttttt tcaagctggg acgcgcacga cacagagaat
    16321 taactaattg aaaaaattaa agattaatta tctgttcatc ggcattctgg cactgctgct
    16381 cgcggtcgct ctctggccat ccattccctg gtttggtaaa gccgacaacc gtatcgccgc
    16441 cattcaagcg cggggagagt tgcgtgtgag caccattcat actcccctga cttataacga
    16501 aatcaacggg aaaccttttg gcctggatta cgaactggcg aaacagtttg ccgattacct
    16561 cggcgtaaaa ctgaaagtga ccgtgcggca gaatatcagc cagctgtttg acgaccttga
    16621 taatggtaac gccgacctgc tggcggcagg actggtctat aacagtgagc gggtaaaaaa
    16681 ttatcagcct ggccctacct attattccgt gtcacaacaa ctggtttata aagtgggtca
    16741 gtatcgccca cgtacgctgg gcaacctgac ggcggagcaa ctcaccgttg caccgggtca
    16801 tgtggtggtt aacgatctcc agaccctgaa agaaacaaaa ttcccggaat taagctggaa
    16861 ggtagacgac aaaaaaggct ctgcggaatt aatggaagat gtcatcgaag gaaaactcga
    16921 ttacaccatt gctgattctg tcgccatcag cctgtttcag cgcgttcacc cggagctcgc
    16981 cgtagcgctc gatatcaccg atgaacaacc ggtgacttgg tttagcccgt tagatggcga
    17041 taataccctt tccgccgccc tgctcgactt cttcaacgaa atgaatgaag acggtacgct
    17101 ggcacgcatt gaagagaaat acctggggca tggcgatgat tttgattacg tcgatacgcg
    17161 cacattttta cgcgccgtcg atgcggtact gccgcagtta aagcccctgt ttgagaaata
    17221 cgccgaagaa attgactggc gtttgctggc cgctattgct tatcaggaat cgcactggga
    17281 tgcacaggcc acttcaccga cgggtgtgcg cggcatgatg atgttaacca aaaataccgc
    17341 gcaaagcctc ggcattacgg atcgtaccga tgccgaacag agcatcagcg gtggcgtgcg
    17401 ttatttgcag gatatgatga gtaaagtgcc ggaaagtgtg ccggagaacg aacggatctg
    17461 gtttgccctc gctgcgtaca atatgggcta tgcgcatatg ctggatgccc gcgccctgac
    17521 ggcaaaaacc aaagggaatc ctgacagttg ggctgacgta aaacagcgtc tgcctttact
    17581 tagccagaaa ccctattaca gcaagctgac ttacggctac gctcgtgggc atgaagccta
    17641 cgcttatgtc gaaaatattc gtaagtatca gattagcctg gtgggttatc tgcaagagaa
    17701 agagaagcag gctacagaag cggcgatgca actggcgcag gattatccgg cggtatcgcc
    17761 tacggagttg ggcaaagaga aatttccttt tctctcgttt ctttcccagt cgtcatcaaa
    17821 ctatttgacc cattctccct ctctgctgtt ttccaggaaa gggagtgaag agaaacaaaa
    17881 ttaatccgtc gaggattgcg cttttttctg cgctttaatt tcctggcggc gcatgcgaaa
    17941 gaagtcactg agcaacgccg cgcactcatc cgccagtatt ccttccgtaa tttccactcg
    18001 gtgattcata cccggatgat gcagcacatc cattaaagat cccgcagcgc cagttttcgc
    18061 gtcacgcgca ccaaagacca cgcgaccaat gcgactgtgg atcatcgctc cggcacacat
    18121 tacacatggt tcaagcgtga catacaacgt ggcgtcgatc agacgataat tttgcatcac
    18181 cagaccaccc tgccgcaggg ccatgatttc tgcatgtgcg gtgggatcat ggcgaccaat
    18241 cgggcggttc cagccttcgc cgattacccg attgttatgc actaataccg cgccgaccgg
    18301 cacttcccgc tcatcccagg cacgtttcgc cagcgtcagc gcgtgacgca tccagtattc
    18361 gtggctaaat tcgacttcag acaaaaagaa aactccggtt ataaaagcgc ggcgcattat
    18421 acacggacgc tatgctttac tctattccag ttgctggagt tcaccgcgcg gggttactcg
    18481 ccaacgatgc tgacagaaat aaagcagcgg attgtcctga ttactgtcgc tatagccact
    18541 gtacagccgc agcggcgtgc cgattttgcg ctccagttgt gcgacctttt catgtcccag
    18601 acaacgcatc gtcaataccc aaccaccata gccacgctga atttggctgg cgataagatt
    18661 aacccgcggc agccagggcg tatcgaaata aaccgcttca accagcggct gcggagagcc
    18721 ggtaatcaac cagatatcag catcggaact taacaggtag gtggttaatc gctcctgaac
    18781 cagcggaaag gcggtaacat tgtcgcgaaa ccagcgcacg aaatcggcct gcaacgtctg
    18841 taaacgtgct tcgctgtgac caaaagtgca cccccacaga agcagactca tcggccagcg
    18901 tgccgcacga ccttttatca ataacgcaat ggctataatc ggtaacaacg gcaggacaag
    18961 taacgcattc agcggttggc gacgcagtaa atagcgcaga aaactgccga acatatcctg
    19021 ctgatgcaat gttccatcta agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc
    19081 atactcctct ggactgaaac atcctgatta attttcgcta ctacatagcc taacagatag
    19141 atcatcactt ttccggcaac aatcgaccgc atcatggcta actggaattt ttaattcatg
    19201 gcaattagcg gcaatggaat ataaaattca ctcgcgtgtg tctcatattg cctgttgtcg
    19261 ccactatttc atcgcgagga aagatatgaa cggcttactt cgtatccgtc agcgttacca
    19321 ggggcttgcc cagagtgata aaaaactggc ggattatctg ctgctacaac ctgatacggc
    19381 gcgccattta agctctcagc aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt
    19441 gaagttcgcg caaaaactcg gctataaagg ttttccggcg cttaagttgg cgttgagtga
    19501 agcgctggca agccagccgg aatcgccctc cgtgcccatt cataaccaaa tccgcggtga
    19561 tgatccgtta cggctggtcg gcgaaaaact gattaaagaa aatactgccg ctatgtacgc
    19621 aacgctaaac gttaatagtg aagagaaact gcatgaatgc gtaacaatgt tgcgctctgc
    19681 gcggcggata attctgaccg gtattggcgc ttcgggtctg gtggtgcaaa actttgcctg
    19741 gaagctgatg aagattggct tcaatgctgc cgcactgcgc gatatgcatg cgctactcgc
    19801 aacagtacag gcgtcgtccc ctgacgatct gttattagcc atttcctaca ccggtgtacg
    19861 acgcgagtta aacctggcgg cagatgagat gctgcgagtg ggcggaaaag tgctggcgat
    19921 taccggcttt actccgaatg ccctgcaaca gcgtgcttct cattgcctgt ataccattgc
    19981 cgaagaacaa gcgacaaaca gtgcttcaat ctctgcttgt cacgctcagg gaatgttaac
    20041 ggatttgctg ttcattgcgc tgattcagca ggatctggaa ctggcaccag aacgtattcg
    20101 tcatagtgaa gcgctggtga aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc
    20161 ccggtttgtg ttgttttgag agtttcctta tggcgctgtt aatcactaaa aaatgcatca
    20221 attgtgatat gtgtgaaatc aaataaaaac acatttattt tcatagtgat acagcaatgt
    20281 gttattgaat gtgttgttgg atttatggtg caaaaaaatt ttgcataagt tcagcaacac
    20341 aaagaacacc actttcttta acgtcctccg cgccctaaag gacggggctt tacggcgcaa
    20401 aaggcctgtg acatacaaca ttaacttttc agtcaaccgt agcacgtcct tgtatacgcc
    20461 gtgtctgcgg ttttatataa ccctggctgg aaacctctta tacaaagtcg attcgccaac
    20521 atcatagatg atcgccacct tctggcgaga aactcctgat gcaattagtc gcccggcctg
    20581 cgcccattat tctggtgaaa gtttgggacg gcgaccgcca attcgtccct gtgcgcgagc
    20641 agcttccagt cctgcttttg ttcgttcaac aatcagttca cgttccattt cagccagggc
    20701 acccatcaca tggaagaaaa agcgtcccat tggtgtgctg gtatcaattg aatcagtcag
    20761 actacgaaag ttgatgcctc gttcgcgcaa ctcctccacc agcacgacaa gatgccgcat
    20821 actgcgcccc agccgatcca gcttccagac aaccagtgtg tcgcctgccg ataatgtcct
    20881 gagcagtttt ttcagccccg gtctttcgga ctttgtacca cttatcttgt cttcaaaaat
    20941 cagctcgcat cctgcacagt tcaacgcatt acgttgtaga tcggtattct ggtcatttgt
    21001 t
//
LOCUS       DADLZM010000065        20717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLZM010000065 DADLZM010000000
VERSION     DADLZM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.065"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..361)
                     /locus_tag="JNY73_002268"
     CDS             complement(<1..361)
                     /locus_tag="JNY73_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY8048270.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPDGDVPQECGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHR"
     gene            complement(636..1728)
                     /gene="gldA"
                     /locus_tag="JNY73_002269"
                     /pseudo
     CDS             complement(636..1728)
                     /gene="gldA"
                     /locus_tag="JNY73_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(1739..2401)
                     /gene="fsa"
                     /locus_tag="JNY73_002270"
                     /pseudo
     CDS             complement(1739..2401)
                     /gene="fsa"
                     /locus_tag="JNY73_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(2413..4914)
                     /gene="ptsP"
                     /locus_tag="JNY73_002271"
     CDS             complement(2413..4914)
                     /gene="ptsP"
                     /locus_tag="JNY73_002271"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8048271.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            5223..6302
                     /locus_tag="JNY73_002272"
     CDS             5223..6302
                     /locus_tag="JNY73_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY8048272.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            6317..6637
                     /locus_tag="JNY73_002273"
     CDS             6317..6637
                     /locus_tag="JNY73_002273"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY8048273.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6688..8985
                     /locus_tag="JNY73_002274"
     CDS             6688..8985
                     /locus_tag="JNY73_002274"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY8048274.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQGRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGHDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8951..9829
                     /locus_tag="JNY73_002275"
     CDS             8951..9829
                     /locus_tag="JNY73_002275"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY8048275.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCSWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9831..10172
                     /locus_tag="JNY73_002276"
     CDS             9831..10172
                     /locus_tag="JNY73_002276"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY8048276.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10159..11010)
                     /locus_tag="JNY73_002277"
     CDS             complement(10159..11010)
                     /locus_tag="JNY73_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8048277.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(11159..12892)
                     /gene="cptA"
                     /locus_tag="JNY73_002278"
     CDS             complement(11159..12892)
                     /gene="cptA"
                     /locus_tag="JNY73_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY8048278.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(13075..15726)
                     /gene="ppc"
                     /locus_tag="JNY73_002279"
     CDS             complement(13075..15726)
                     /gene="ppc"
                     /locus_tag="JNY73_002279"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY8048279.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(16078..17229)
                     /gene="argE"
                     /locus_tag="JNY73_002280"
     CDS             complement(16078..17229)
                     /gene="argE"
                     /locus_tag="JNY73_002280"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY8048280.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPTRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            17383..18387
                     /gene="argC"
                     /locus_tag="JNY73_002281"
     CDS             17383..18387
                     /gene="argC"
                     /locus_tag="JNY73_002281"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY8048281.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            18395..19171
                     /gene="argB"
                     /locus_tag="JNY73_002282"
     CDS             18395..19171
                     /gene="argB"
                     /locus_tag="JNY73_002282"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY8048282.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            19232..20605
                     /gene="argH"
                     /locus_tag="JNY73_002283"
     CDS             19232..20605
                     /gene="argH"
                     /locus_tag="JNY73_002283"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY8048283.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         4711 a         5233 c         5782 g         4991 t
ORIGIN      
        1 gacggtgatc gatgttgctg tccgggcgct ttaacttcca tttttgcggg tactcagcaa
       61 aattcttcgc catatcttcg tgaaccagtt tctgcaaatc gaccttctgg ctgcgcaatg
      121 cgcggatcac cacatcggaa catacaccgc attcttgcgg aacatcaccg tcaggatagg
      181 taagctgcat atacgccgga tcgtaaaata gcgtgctgcc aatttgctgt ctggcaccgt
      241 ctgcgatggc aaggttggta ttggcctgga tttgtaccac ggttggtgga acggcgggag
      301 attttaagga gtggctggta aatgccgtta gcaggctgag cagcgccagt gaagctttca
      361 tctggttcgt ccgtgaaaat aagagtcaca gattcgacct tccgggctaa cgattgttct
      421 gtggagaaaa tgtccagaag cggataaaaa actcctgaac aacgtgggag tttcagatta
      481 ctggcacaga gtgccggatg cggagtgaac gccttatccg gcctacaaaa gcacgcaaat
      541 tcaatatatt gcagagattg cgtaggcctg ataagcgtag cgcatcaggc aattttgcgt
      601 tcaaactccc ggacaagccg ggagtttgga gtaggttatt cccactcttg caggaaacgc
      661 tgaccgtact ggtcagctac cagcagtgcg gcgtaaacct gatctggcgt cgcgccgcca
      721 ggcatgttgt ggatggtttc accttctgca caagatgctt ttgcaacaat gcgcattttc
      781 gccgggacat cttctttaat atccagttga gcgagagtta ttggcaaacc taccgcatgg
      841 ctaagtgcag ctacggtttc gatttcctcc accggcgcgt tttccagtac cagctgcgtc
      901 agcgtaccaa atgccacttt ttcaccgtga taatagtgat gcgcgtccgg gatagcggtc
      961 aggccgttat gcaccgcgtg agccgccgct ttcaaaacca acgccgctca aataggtgtt
     1021 cgcttcaatc acgcgctcca gcgccggggt tactacatgc tgttcggcgg caagcatcgc
     1081 tttttcgcct tcttccagca gggtgttgta gcacagttca gccagtgcca gcgccgcctg
     1141 ggtgcacttg ccgcccgcca tggtggtcgc gccgctacga gagcaagcac gcgcttcaaa
     1201 ccaggttgcc agcgcatcgc cgatacccgc tgctaacaga cgtgcaggtg cgcccgcgac
     1261 gattttggtg tcgacaatga ccatattcgg gttatttggc aacagcagat agcggtcaaa
     1321 ctcaccctca tcggtgtaga taacagacaa tgcgctgcac ggtgcatcgg tagaggcgat
     1381 agtcggtgcg atcgctaccg gaacacccat gaaatgtgcc agtgctttgg cagtatcgag
     1441 ggtttttccg ccaccgatac cgagaattgc gccacactgc gcagtctccg cgatgccacg
     1501 cagacggtcg atctcatttt gcgaacattc accgccaaac ggcgtaattt ctactaccag
     1561 tccagcatct ttaaagcttt tctcgacagt ggattgagca aaacctaaaa caaatttgtc
     1621 acccaccact aaccagcgtt ctgccagcgg cttcaggtat tcgcccagac gattaatcac
     1681 atcagcgccc tggatgtatt tacccggtga ttgaataatg cggtccataa ttgctccttt
     1741 agagatgagt agtgccaaat gcggcattcc agtcgtgttc gaacttctct atagctgact
     1801 ctactgcagg ggtgttgagc atttgttgcg ctacatctaa gggcagggtg atggattcac
     1861 atcctgccag taaacagtcc agcgcctgac acggcgtttt aaagctggct gccagcacca
     1921 tgctttctgg cgcgtgcatt tctaacagcg tttgcagctc ctgaaccgta cgaatgccgt
     1981 ccccgcccta ggcatctacg cggttaacat acggagcaac gtattttgcc cctgccagtg
     2041 cggctaataa cccttctgcg gcgctatata cagcggtgcc aagtgtagta ataccctctt
     2101 ttttcagtat tttaattgct gccagacctt cggaagtcac cgggattttc accacaatac
     2161 ccggaatagc gtcgcgcagg cgcttcgctt cttccaccat cccctgcgcg tcgcggctca
     2221 tggtctgagc aaacagaatg ccctcatcac caatcgcttt ttgcagacgc ggcagcactt
     2281 cccagatgga ctccttgctg gcagcgataa tgctcgggtt agttgtcacc ccggcaatcg
     2341 ggaatatgcg tgccagacgt tcgacttctg cgacgttagc ggtgtccaga tacagttcca
     2401 tgatgtttcc ttttacagtt ccagttcatg ttgcagcagg ctggcgatag cgtcggcaga
     2461 ggcggcgtta accagcgcgt tacggaattc ttcgtgcatg atgcggcgag cgaggcgcga
     2521 gaaaatgcgc atgtgctgat cgcccgcagc gtgtttgttc agggttaaca taatgatgaa
     2581 ttgcgcttca tcatcgcccc agcgcaccgg cgcttgcaga cgcgccacgc tgatggtgga
     2641 ttgctcaatg tgttctgatt tgctgtgtgg aatggcaaaa ctaaagccca gaccggtaga
     2701 gaaaacggcc tcgcgcgccc ataagtcggc ttccagttta cgcggatagc ggcagcggcc
     2761 cgccagcagc aggttatcgg tcatgccttt gagcacttct tctttgctgc gccagtcact
     2821 ttccagcgtg atgcactcgg cggtgaccag cggtgcgtcc tgttgggtca tgcggaattg
     2881 cgccagcagg tgttccactt ccagcgaagt acggcaggcc attgcctggt tgagcaactt
     2941 gcggcactca cggctatcaa gttgcgccat ccgcgctttc gccgccggaa ttgatggtgc
     3001 gctcatgctg agttcatcca gccctaagcc gaccagcaac ggcagcacgg aacctttcgc
     3061 tcccagctca ccgcacagac caatccattt accctggcga tgcaccgcct gcacggcgta
     3121 atcgagcgcc cgcaagaatg ccggattcag gctgttgtag tgacgagtaa ccttagcgtt
     3181 atcgcgatcc actgccagca gatattgcgt caggtcatta ctaccaatac taaagaaatc
     3241 aatctcttcg cagcattgat cgatgatgaa catcaccgac ggcacttcca gcataatgcc
     3301 gagctggatc ttctcatcaa acggaatgtg ttcgttacgt agttgctgtt tggcttctgc
     3361 cagtttttct ttcacccata agatctcttc cattgaggag atcatcggga tcatgatttt
     3421 caggctgccg tgagcggagg cgcgcaggat cgagcgtagt tgtgtggtaa ataacgacgc
     3481 gtattcttca taaatacgca cggcgcgata accgaggaac gggtttgcct ctgcgggaat
     3541 gtttagataa tcaacgggtt tgtcgccgcc aatgtccata gtgcgcacaa taatgctgcg
     3601 tccgttggca gactccagcg cctgacaaaa aatgttgtac agctcgcttt cgcccggtgc
     3661 actggtgcga tccatataga gcatttcagt gcggaacaaa ccaacgcctt ccgcaccatt
     3721 gccgaatgcg gcctgcgctt ccacggagtg agcgatgtta gcggcaattt caatgcggat
     3781 accgtcagcg gtacgggctt gttgggtcag ccagacacgc tgttgctcac gcagggcgtc
     3841 ctgtacgcgg gcttcttgct gataataacg agctacggct tctcctggct caaccacaat
     3901 cgccccggcg ttgccgtcga tataaatcgt ttgatgctgc cacggagtga gggcatcaat
     3961 atccacacca accagcgtcg gaatgttgaa cgaacgggca aggatcaccg tatgtgaggt
     4021 ggtgccgccg cttttcagta acaatccttt gaggtgattt ttatccagtt cgaggaactg
     4081 gctgggggtc agttcatcag ccatacaaat ggcggactgc gtcagtttgc ccggtgccgg
     4141 gaagcgttgc tcaccgtaga tttgctggag taactggaag cagacgtcgc gtacgtccag
     4201 ggcacgttct tgcaggtatc tgctgctgga acgggcgaac tcttcgcaaa agtgattcgc
     4261 actggcgaca atcgcttcgg cgcagcttaa tccggcgctg actcctgcca gtaaatgttc
     4321 gtgcagggaa gtatcgccag ccagggatcg gtgcgcttcc agaatcgcgc tggtagcacc
     4381 gtcgctatcc agcagacgaa actcaatgtt tttcagtacc agcgtcaggc cgttttccag
     4441 tgcggattgc tcggcgtcaa cgtctttggc tgcgggaaga ttactcagcg cattgagatc
     4501 taaagaagag atcggcgtca gaatgccgcc tgcactaccg ctgcacacgg tgcgggcgcg
     4561 gataatttgc ggattcagat tggtcagtga aaccggcagt ggttccagtt cgtcagattt
     4621 aacttccgcc agcggcgcat cgcagtgggg gaattcatcg cgcagccatt ggcttaaacg
     4681 ctggtgccct tcctgttcgt cggccccgga aatcagtagc tggcagttat cacccaccag
     4741 cgtatcggtg ccaatcagcg ccagagcgct tttggcgttg cctttacggt cagtacgcag
     4801 gttatgccac tcaatttgag atgaaaaagt attacacagc gtttcaacgt ggcttgccgg
     4861 acgcgcatgt acgccgttag gtagctcaca aataaattcc acaatcaggg ccattgcctt
     4921 tctcccggta cggttttctg tttacaggat aagagcgcac ggtaacggcc tgccatgtga
     4981 caaatctgcc aaaagctgga caaatgtaat gtaaccgtca atttgcgacg cgtctcacaa
     5041 gacgccgttt tgcggcatgc ttccggatta tcgcaagtta tgaggcggat cgcatttttg
     5101 tactgatatt acaaaagtcc agtaaatggc ctttttatcc actgtttgct ccgcctgcga
     5161 ttgcctattg tttcctccat caaaaatatg gacatgggct tttgcaagcc cgggagcagc
     5221 ttatgaatga gttggtgcag atcctgaaaa atacccgtca gcatttaatg acgggcgttt
     5281 cacacatgat tcccttcgtg gtatcgggcg gtattttgct ggcggtttcc gtcatgttgt
     5341 atggcaaagg cgcagtgccg gatgccgtag ccgatccgaa tttgaaaaaa ctgtttgata
     5401 ttggcgttgc aggtttgacg ctgatggtgc ctttcctcgc cgcttacatt ggttactcca
     5461 ttgcagagcg ttctgcgctg gctccgtgcg ctatcggtgc ctgggttggt aacagctttg
     5521 gtgcgggctt ctttggtgcg ctgatcgccg ggattatcgg cggcatcgtg gtgcattacc
     5581 tgaagaaaat tccggtgcat aaagttctgc gctcggtgat gcccatcttc attattccta
     5641 tcatcggcac actgattacc gcaggcatca tgatgtgggg cttgggcgag cctgtagggg
     5701 cgttgaccaa cagcctgact cagtggcttc aggggatgca gcagggcagc attgttatgc
     5761 tggcggtgat catggggctg atgctggcgt tcgatatggg cggtccggtt aacaaagtgg
     5821 cctatgcctt catgctgatt tgcgttgctc agggtgttta taccgtggtg gccattgctg
     5881 ccgtgggtat ttgtatcccg ccgctgggga tggggctggc gacgctgatt ggtcgtaaaa
     5941 atttctccgc agaagagcgc gaaaccggca aagcggcact ggtgatgggc tgtgttggcg
     6001 ttactgaagg ggcgattcct ttcgccgctg ccgatccgct gcgtgtcatt ccttccatta
     6061 tggtcggttc agtttgtggt gcagtaactg cggcgctggt cggtgcgcag tgctatgcag
     6121 gctggggtgg tctgattgtg ctgccggtgg ttgaaggtaa gttgggttat atcgcagcag
     6181 tggctgtcgg agcagtggtg acggctgttt gtgtgaacgt gctgaaaagt ctggcgcgta
     6241 aaaatgggtc ttcgactgat gaaaaagaag acgacctgga tttggatttt gaaattaatt
     6301 aattgaggaa cgagccatga cgaaaattat tgcagtaacc gcatgtcctt caggtgttgc
     6361 ccatacctat atggcagcag aggcgctgga aagtgccgcg aaagcgaaag gctgggaagt
     6421 gaaagtagaa acccaggggt caattggtct ggaaaatgaa ctgactgcgg aagacgtggc
     6481 gagcgctgat atggtgattc tgaccaaaga tatcggcatc aagtttgaag agcgttttgc
     6541 tggcaagacc atcgtgcgcg tcaacatcag cgatgcagta aaacgtgctg acgccattat
     6601 gagcaaaatt gaagctcacc tggcgcaaac cgcttaagtt cttctcccgc tcgcaagggc
     6661 gggttcgctt tcccacagga gttcctcatg acgaatcgta tctctcgcct caaaactgca
     6721 ctgtttgcca atacccgtga aatctcgctg gagcgggcgc tgctttatac cgccagccat
     6781 cggcaaaccg aaggcgaacc ggtgatattg cgccgggcga aagcaacagc gtatatcctt
     6841 gaacatgttg aaatttcgat tcgtgatgaa gaactgattg ccggtaaccg caccgtaaaa
     6901 ccgcgcgccg ggattatgtc gccggaaatg gacccttact ggctgctgaa agagctggat
     6961 caattcccga cgcgtccgca gggccgcttt gctatcagcg aagaagataa acgtatctac
     7021 cgtgaagagt tgttcccgta ctgggaaaaa cgttcgatga aagatttcat caacgggcag
     7081 atgacagatg aagtaaaagc cgcgaccaac acgcagattt tcagcatcaa ccagacggat
     7141 aaaggccagg ggcacattat tattgattac ccacgcctgt tgaatcacgg tctgggtgag
     7201 ctggtggcac agatgcagca acattgtcag caacagccgg agaatcactt ttatcaggcc
     7261 gcgttgttac tgctggaagc ctcgcagaaa cacattttgc gttacgccga actggcggaa
     7321 acgatggcgg caaactgcac agatgcccag cgtcgcgaag agctgctgac tattgcggag
     7381 atctcccgcc ataacgcgca acataagccg cagacgttct ggcaggcgtg ccagttattc
     7441 tggtacatga acatcattct gcaatacgaa tccaacgcca gttcgctgtc tttagggcgc
     7501 ttcgaccagt atatgttgcc gttctatcag gcatctttaa cccagggcga agatccggcg
     7561 ttcctgaaag aactgctcga atctttatgg gtgaaatgca acgacatcgt gctgttgcgc
     7621 tccaccagta gcgcgcgtta tttcgccggt ttcccgaccg gctataccgc actgctcggc
     7681 gggttaaccg agaacggacg tagcgcggtg aacgtgcttt cgttcctttg ccttgacgcc
     7741 tatcaaagcg tgcaattacc gcaaccgaac ctcggcgtgc gcactaacgc cttgatcgac
     7801 acgccgttcc tgatgaaaac cgccgaaacc attcgcctcg gcaccggtat tccgcaaatc
     7861 tttaacgatg aagtggtggt gccagcgttc ctcaaccgtg gcgtttcgct ggaagatgcg
     7921 cgcgactatt ccgtagtggg ctgtgtggaa ttatctattc ccggcagaac ctacggcttg
     7981 catgacatcg cgatgtttaa tctgctgaaa gtgatggaaa tctgcctgca tgaaaatgaa
     8041 ggcaatgctg cgctgactta tgaaggttta ctggagcaga tccgcgccaa gatcagccac
     8101 tacatcaccc tgatggttga gggcagcaat atttgtgata tcggccatcg cgactgggca
     8161 cctgtaccgc tgctctcgtc ttttatcagc gattgtctgg aaaaaggtcg cgatattacc
     8221 gatggcggcg cgcgttataa cttctccggc gtacagggaa ttggtatcgc caacctgagc
     8281 gattctctcc atgcgttgaa agggatggtt tttgagcaac agcgtttaag ttttgacgaa
     8341 ttgctgtcgg tattaaaagc caactttgca acgccagaag gcgaaaaagt ccgcgctcgc
     8401 ttaattaacc gctttgagaa atacggtaac gatatcgacg aggtggataa cattagcgcc
     8461 gaactgttgc gccactactg caaagaagtg gaaaaatacc agaacccgcg cggcggttac
     8521 ttcacgccgg gatcgtatac cgtttctgct cacgttccgt tgggatcggt ggttggcgcg
     8581 acgccagacg gtcgttttgc cggggaacaa ctggcagacg gcggcttgtc acctatgctg
     8641 ggtcacgacg cacaagggcc aacggcggta ctgaagtcag tcagtaagct cgataatacg
     8701 ctgctgtcta acggtacgtt gctgaacgtg aaattcactc cggcgaccct ggaaggtgaa
     8761 gcgggattac gcaaactggc cgacttctta cgggtgttta cccagcttaa gttgcagcat
     8821 attcagttta acgtggtgaa cgccgacacg ttgcgggaag cgcaacagcg cccacaagat
     8881 tatgccgggc tggtggtgcg cgttgctgga tacagcgcct tctttgtcga actgtcgaag
     8941 gagatccagg atgacatcat ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg
     9001 ccgcgatgat gtggcgcgca ttttcaacat tcagcgttat tcactgaatg acggcgaggg
     9061 cattcgtacg gtggtctttt ttaaaggctg tccgcatctt tgctcgtggt gtgctaatcc
     9121 ggagtcgatc tccggcaaaa tccagacggt acgcagagag gcaaaatgtc tgcactgtgc
     9181 gaaatgtttg cgtgatgcgg atgaatgccc ctccggggcg tttgaacgga ttggtcgcga
     9241 tatcagcctt gacgctctgg aacgggaagt gatgaaagat gacattttct ttcgcacgtc
     9301 cggcggcggc gtcacgcttt ctggcggcga agtgttaatg caggcggagt ttgctacccg
     9361 ttttttacag cgactgcggc tgtggggtgt ctcatgcgcc attgaaactg ccggagacgc
     9421 gccagcaagc aagctgttac cgctggcgaa attgtgcgat gaagtgctgt tcgatttaaa
     9481 aattatggac gcgactcagg cgcgggatgt ggtgaagatg aacctgccac gcgtgctgga
     9541 gaatctgcgt ttgctggtga gtgagggtgt caacgtgatc ccgcgtttac cgttgatccc
     9601 tggtttcacg ctcagccggg agaatatgca gcaggcgctg gatgtactga tcccgctgaa
     9661 aatcaggcag atccatctgt taccgtttca tcagtacggc gaaccgaaat accgcctgct
     9721 ggggaaaaca tggtcgatga aagaggtgcc tgcgccgtcg tcagccgatg tgacaacgat
     9781 gcgcgaaatg gcagaacggg ccggatttca ggttaccgtg ggaggttaaa atggcatacc
     9841 tggtggcagt aaccgcctgc gtaagcggcg tggcgcatac ttatatggcg gcggaacggc
     9901 tggaaaagtt gtgccagtta gagaagtggg gcgtcagcat tgaaactcag ggcgcgctgg
     9961 gaacggagaa tcgtttagcg gacgaggata tccgtcgggc ggatgttgct ctgttgatta
    10021 cggatatcga gcttgccggt gccgagcgat ttgaacattg ccgctatgtg caatgcagca
    10081 tctacgcatt cctgcgtgag ccgcagcggg taatgagcgc ggtgcgcaaa gtgctttctg
    10141 cgccgcagca aacccatctt attctggagt agtcggtttt tctgtcagtt ggctgtggta
    10201 ctgccggcga tattccgacg gcgagtgttc ggtgttttta cgaaacagac ggcagaagta
    10261 gttgctgtcg acaaagccgc aggcgtgcgc cacttctttt accttcaggt catagccttt
    10321 caataacgtc ttagcgtgct ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc
    10381 ccccgttttt tgaaacaggt gcgagagata gtttggtgaa atatagaatg cctgtgcgac
    10441 agattcgcgg gtaagcgcgg aggcatagcg ttcgtcgata taatcgcgaa tagcttcaaa
    10501 tagtgcctgg ctgcgtgagg cggtctggat ttggctgccg cgcaaatcgc ggcagtggct
    10561 aagcaggctg gctacgataa gccttgccgt ttgctgctcc tgcggctgca tttgcatttc
    10621 attgagcgtt tgtagcagaa aagaacctat gcgtgggccg cgtcgggcga cgtgttgctt
    10681 cgccagattt tgatattgtt tgccatccca ttgcacgacg ctgaagccga gttgctgttt
    10741 gccaaacagc acgctaaagg tggtagcggg tgttttccat tgtgggaaat tccagccacc
    10801 agcggcaacg tacagcacat cgccgggaac taacgttgct ccagcgccgg tatcaacaaa
    10861 ctcaccttcc agcacaattt ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac
    10921 cgggccgtta ctgctggcaa agtaaatctg gcgcagggac agcgggccgt tgatcaggcg
    10981 ggagagcaga tagctgacgt cgtgatacat gttattccct taggtatgta gacgggaaaa
    11041 cagtaactgg cattattaaa aaaggccacc tgaaaagtgt ggcctgaggg gagttcgatg
    11101 taaacattgc ggcagtatcg catccggcaa atagcgccag acgctataca aaggtcatct
    11161 actgattacc cacctgatcg ccatacggca gagtgtcgta atcgatcagc gcgtttttct
    11221 tgtacgggtt accaatccag cgggtagttt ctttgaactg cggattcacc actgaacggg
    11281 ttggatcgta accgtcgtaa gataagcccg ccaaatctga ccaggtgtgg atcagttccg
    11341 ccaggctgta tttacgatcg acatcctgtg agaaatcacg gggatgagtc gcttgccatt
    11401 tttccgacgt ccacagcagg aacggaatgg tgtacatgtg gcgcgtcggg ttgtcttcgt
    11461 tacgcccctg agttttgtgc ggcggcgtgt cgtaaacctc ttcaccgtgg tcagagaagt
    11521 aaaccaggaa tccgttcggg tctgccgctt taaagtcttt aatcaggctg gcaaccacat
    11581 gatcgttata caggttagcg ttgtcataat cgttatatga ttccagctct tctgcgctta
    11641 atcctggagg aacatgatcg gtattgccat caaacttgcc ctgattttcc gggtagcggt
    11701 atttgtattt gatatgcgta cccagcagat gaacgatgat cagtttcttc ggcgcagggt
    11761 cattcagcac atcctggaac ggcttcagca cgttggtgtc gtattcacgc gcactctgcg
    11821 tacgttgctg gttcatgtag tactgcttgt cggtctggcg cgaaaatacc gtcagcatgg
    11881 tattgcgggc ggtcatcgtc tgctggttgg tgatccagaa ggttttataa cccgcctgtt
    11941 tcatcatgtt catcagcgac ggctgcgtca gatacagatc cgggttcttt tcattggcaa
    12001 aggtcagcgc ctgttgcagg atttcaatgg tgtacggacg agacgtaact acgttattaa
    12061 acacggtcag attcggatcg gttttatgca gcgcatccag ctccggcgtg gtttcacgcg
    12121 gataaccgta cagactcatg cgtccgcgct gggtcgactc gccaatcacc agcactaaag
    12181 tgcgcggttc gttacccgat tcatctttga aattagccag tggcggcaag gcattatttt
    12241 cattcagtaa ctttgttagc gagtttagtt gctgacgata ctgataatag ccggtcagga
    12301 attgccacgg tgcggcaggc tccatacgcg aggctaggtt atccaacgtt ttctcaaacg
    12361 gcttgttttt gataaacgta ttcatggcga tcggatgcag aatcaagcca taaagcaggg
    12421 caaaagagac aacataacgc cacggctttg gaatatagac cgggcgcagg cgtgtccaca
    12481 gcagaactgc caccgccgta taggccagcg cgataagcac aattttcagg ctgaaatact
    12541 ggcttaaata ctcgctggct tcgttggtgt tggtttcgaa catcacaaac agaacgctct
    12601 gcgagaactc ctgcccgtag atgacgtagt agcacagcgc cgccagagag gccgtccata
    12661 gcaccacgcc gatcactgcg gcaataattt taatccgctt cggaaagagg aataccggga
    12721 tcaaccacag cgagctgaat aacagcgagt cgcgaatgcc gttggtgcca ctataaccac
    12781 tgatgtaaat aatggcctgt agcagagtag agaaaaacca aaagtagagc agtgcccaac
    12841 ccagggcttt ccagctagaa aggggtttag cctggacttc tgtggaatgc atagtaagaa
    12901 cctgtcttga aaaaaatatc gccgaatgta acgacaattc cttaaggata tctgaaggta
    12961 tattcagaat ttgaataaaa tgcagacaga aatatattga aaacgagggt gttagaacag
    13021 aagtatttca gaaaaccctc gcgcaaaagc acgagggttt gcagaagagg aagattagcc
    13081 ggtattacgc atacctgccg caatcccggc aatagtgacc attaacgctt gttcgacgcg
    13141 aggatccggt tcctggcctt ctttttctgc ctggcgggag cggtgcagca actcggcctg
    13201 caatacgttc agcgggtcgg tgtaaatatt ccgtagctga atagactctg caatccacgg
    13261 cagatcggcc atcagatggg aatcgttggc aatcgccagc accactttga tgtcttcttc
    13321 ttgcaggttg cgtaactctt tacctaacgg ccacagtgct ttgtctacca gacgttgatc
    13381 atagtattcc gccaaccaca ggtctgcttt ggcgaagacc atctccagca tgccgagacg
    13441 cgtcgagaag aatggccaat cgcggcacat ggcttccagc tcgctctgtt tgccatcttc
    13501 gaccactttt tgcagcgccg tacctgcacc tagccaggcg gggagcatca ggcggttttg
    13561 cgtccaggca aaaatccacg gtatggcgcg cagcgactcg acgccgccgg ttgggcgacg
    13621 tttcgccgga cgtgaaccca acggcagttt gcccaattct tgttccggcg tagcggagcg
    13681 gaagtaaggc acaaaatctt tgttttcacg tacgtagcca cggtagagat cgcaggagat
    13741 gactgacagt tcatccataa tgcgacgcca gctctctttc ggctcaggtg gtggcagcag
    13801 gttggcttcc agaatcgccc cggtgtaaag cgacaggctg ctgacggtga tttctggcag
    13861 accatattta aagcggatca tctcgccctg ttcagtcacg cgcaggccac ctttcaggct
    13921 tcctggcggt tgtgacagca gcgccgcatg ggcaggtgcg ccgccgcgac caatggaacc
    13981 gccgcgaccg tggaacaacg tcagctcaat acccgctttt tcgcaggttt tgattaatgc
    14041 atcctgtgcc tgatattgcg cccaggaagc tgccatcacg cccgcatctt ttgctgagtc
    14101 ggaatagcca atcatcacca tctgtttgcc ctgaatcagg ccgcgatacc agtcgatatt
    14161 gagcagctgg gtcatgacat cgttggcgtt gttcaggtca tcgagggttt caaacagcgg
    14221 agcaaccggc atcgcaaacc cgatacccgc ttctttcagc agcaggtgga cagccagtac
    14281 gtcggacggc gttttcgcca tcgagatcac gtaggcggca atggagcctt gcggcgcttc
    14341 ggcaatcacc tggcaggtat cgagcacttc gcgcgtttcg gcgcttggtt gccagttgcg
    14401 cggcagaagc ggacgtttgg agttcagttc gcggatcagg aacgcctgtt tgtcggcctc
    14461 tgaccagctt tcgtagtcgc cgataccgag gtagcgggtc agctcgccca gcgcttcggt
    14521 atggcgggtg ctctcctgac ggatatcaat acggaccagc ggtacgccga aacatttcac
    14581 gcggcgcagg gtgtcgagca gatcgccgtt ggcgataata cccatgccac acgcctgaag
    14641 tgactggtag caagcgtaga gcggttccca cagttcttcg ttttgtgtca gcagcccttc
    14701 tggttttggc agttcttcgc ctttcaggcg cgcttccagc catgcctgtg tcgccatcag
    14761 gcgagaacgc aggtttttca tcagatagcg atacggttct gcggcacctt cttcgccaac
    14821 cagcgccagc agttcagggg tcgcttcaac catcgacagt tcagaaacca gaacctgaat
    14881 atctttcagg aacagatcgg tggctttcca gcggctgagc agcaggacgt ggcgggtgat
    14941 atcggcagtg acgttcgggt tgccgtcgcg gtcaccgccc atccacgagg taaaacgtac
    15001 cggaacaaat tcgacgggca gtttgtagcc gagattctct tccagttgtt cgttcagttc
    15061 gcgcaggtaa ttcggtacgc cttgccacag gctgttttct actacggcaa agccccattt
    15121 ggcttcatct accgggcttg gacgcagctt acggatttca tcggtatgcc atgactgggc
    15181 gatcaactgg tgcaggcgac gcatcagctg gttgtgttcg tagtcagcga tatctttgtt
    15241 atcgagctgt tttaaacagg cgttcacttc caccattttg tggatcagtg tacgacgggt
    15301 aatttcggtt gggtgagccg tgaggaccag ttccagcgac agcgattcca ctgctttttt
    15361 gatggtgtct tcgctcagtt ccggctggtt tttcagttta cgcagggtgc gggcgatcac
    15421 ttccgggttg ctggcagctt cgcctttcgg cgaaatgctg tggtattgct cggcggtgtt
    15481 ggccaggttc aggaactgac taaacgcacg cgcaacgggc agcagctcgt cgttcgacaa
    15541 attttgtaag gtggtgagca actcctggcg gttagcatca ttgccagcgc gtgaagattt
    15601 cgacaactta cggatagttt ctacgcgttc aagaatgtgt tctcccaacg catccttgat
    15661 ggtttctccc agcactttgc cgagcatact gacattacta cgcaatgcgg aatattgttc
    15721 gttcatatta ccccagacac cccatcttat cgtttgatag ccctgtatcc ttcacgtcgc
    15781 attggcgcga atatgctcgg gctttgcttt tcgtcgtctt ttataaagcc acgtaaaagc
    15841 ggtgacgtca aatgctgcga aatcgcttca gcaaacgaat aaatagcagg aatttacgcc
    15901 attaaattca cgacgcttta aataagcgta acttatggaa atgttaaaaa atcgccatac
    15961 ataacaccaa aggtgtaggt cggataagat gcgcaagcat cgcatccgac attattgcgg
    16021 caccggagtt tggcaacggt gccggatgcg gcgcgagcgc cttatccggc ctacgtttta
    16081 atgccagcaa aaatggtgaa ttacctggat aatcaactca cgggtgggtt taataaaccg
    16141 cgtctccaga tattcatcag gctgatgagc ttgattgatg gaacccggac cgagcaccag
    16201 cgtcgggcat aacgtttgaa taaacggcgc ttcggtacag taattcacca cttcggtttt
    16261 tgctccgagc aatttctcaa ccacttcaac cagttgatga ttcggtgggc attcatagcc
    16321 agggatcggc ggatgcagct cgtcgaccgt cagacgaccc ggccagcgtt cgctcaccgg
    16381 agccaatgca tcgttgagca aaccattaag ttcattgagt gtcatgccag gctgcggacg
    16441 aatatccata tgcaactcac agcaagcgca aatacggtta gaagcgtcgc caccgtgaat
    16501 atgcccgagg ttgagcgtag ggtatggcac ggtaaatgct tcgtagtgat aacgttcttt
    16561 caggttatcg cgcaattgca aaatatgccc gatggcgtcg tgcattagtt cgatagcgtt
    16621 aactccgcgt gttggatcgc tggagtgccc cgactggccc tgaatacgga tggcgttaga
    16681 gatatgacct ttatgtgcgc gtaccggttg tagtgacgtc ggttcgccaa tgatggcgca
    16741 atccgggcgc agggcggtag tttcggcaaa ataacgcgct ccggccatac tggtttcttc
    16801 atcagcagtc gccagaatgt agagcggttt tttcagtttc gtgacgtcga catcgcgtag
    16861 cgcatcaagg ataaacgcaa aaaagccttt catgtcggcg gtgcctaagc cgtaaagctt
    16921 gccgtcatgc tccgtcagtg taaacggatc gcgcgtccag cgaccgtcat caaatggcac
    16981 cgtatcggta tgccccgcca gcaacaagcc gccagccccc tgtccgtaac tggccagcat
    17041 attaaatttg ttgcgagttc ctggaacagg ctgcacttcc acattgaagc ccaaatcttt
    17101 aaaccagtcc gccagcagag tgattaaatc tgcattgctt tgatcgagtg cctcttccgt
    17161 agcgcttatt gaaggtgtgg caatcagagc gcggtaaatc tcgataaatg gcggtaattt
    17221 gtttttcatt gttgacacac ctctggtcat gatagtatca atattcatgc agtatttatg
    17281 aataaaaata cactaacgtt gagcgtaata aaacccacca gccgtaaggt gaatgtttta
    17341 cgtttaacct ggcaaccaga cataagaagg tgaatagccc cgatgttgaa tacgctgatt
    17401 gtgggtgcca gcggctacgc tggcgcagag ctagtgacct atgtaaatcg ccatccgcat
    17461 atgaacataa ccgctttgac tgtttcagcg caaagcaatg atgcgggaaa gttaatctcc
    17521 gatttgcatc cgcagctaaa aggcatcgtt gagctgccgt tgcagccgat gtcggatatc
    17581 agcgagttta gcccaggggt ggacgtagtg tttctcgcca ccgcccatga agttagccac
    17641 gatttagcgc cgcagtttct tgaagcgggc tgcgtggtgt tcgacctttc cggcgcgttt
    17701 cgtgttaacg acgccacctt ctatgaaaaa tattacggct ttacccatca atacccggaa
    17761 ctgctggaac aggcagccta cggtctggcg gagtggtgcg gtaataaatt aaaagaagcg
    17821 aatttgattg cggtgccagg ctgttatccg acggcggcac agctggcgct gaaaccgttg
    17881 attgatgccg atcttcttga cctcaatcag tggccggtga tcaacgccac cagcggcgtg
    17941 agcggtgcag gacgtaaggc agcgatttca aatagttttt gtgaagttag cctgcaaccg
    18001 tatggcgtct ttactcatcg ccatcaacca gagatcgcca cacacctcgg tgctgacgtt
    18061 atcttcaccc cacatctggg caatttcccg cgcggcattc tcgaaaccat tacctgccgc
    18121 ctgaaatcgg gtgtgaccca ggcgcaagtc gcgcaagcgt tacagcaggc gtatgcccat
    18181 aaaccgctgg tgcggctgta tgacaaaggc gttccggcgc tgaaaaatgt cgttgggctg
    18241 ccattttgcg atatcgggtt tgccgttcag ggcgagcatc tgattattgt ggcgaccgaa
    18301 gacaacttac tgaaaggcgc ggcggcacaa gcggtacagt gcgccaatat tcgtttcggc
    18361 tatgcggaaa cgcagtctct tatttaaggg tgcaatgatg aatccattaa ttatcaaact
    18421 gggcggcgta ctgctggata gtgaagaggc gctggaacgt ctgtttagcg cattggtgaa
    18481 ttatcgtgag tcacatcagc gtccgctggt gattgtgcac ggcggcggtt gcgtggtgga
    18541 tgagctgatg aaagggctga atctgccggt gaaaaagaaa aacggcctgc gggtgacgcc
    18601 tgctgatcag atagacatta tcaccggagc actggcggga acggcaaata aaaccctgtt
    18661 ggcatgggcg aagaaacatc agattgcggc cgtaggtttg tttctcggtg acggcgacag
    18721 cgtcaaagtg acccagcttg atgaagagtt aggtcatgtt ggactggcgc agccaggttc
    18781 gcctaagctt atcaactcct tgctggagaa cggttatctg ccggtggtca gctccattgg
    18841 cgtaacagac gaagggcaac tgatgaacgt caatgccgac caggcggcaa cggcgctggc
    18901 ggcaacgctg ggcgcggatc tgattttgct ctccgacgtc agcggcattc tcgacggcaa
    18961 agggcaacgc attgccgaaa tgaccgccgc gaaagcagaa caactgattg agcagggcat
    19021 tattactgac ggcatgatag tgaaagtgaa cgcggcgctg gatgcggccc gcacgctggg
    19081 ccgtccggta gatatcgctt cttggcgtta tgcggagcag cttccggcac tgtttaacgg
    19141 tatgccgatg ggtacgcgga ttttggctta agttttgtta gccggaggcg cagctttccg
    19201 gcattgaatt tcaaaataag gaaacagagt tatggcactt tggggcgggc gttttaccca
    19261 ggcagcagat caacggttca aacaattcaa cgactcactg cgctttgatt accgtctggc
    19321 ggagcaggat attgttggct ctgtggcctg gtccaaagcc ttggtaacgg tcggcgtgtt
    19381 aaccgcagaa gagcaggcgc aactggaaga ggcgctgaac gtgttgctgg aagatgttcg
    19441 cgccaggcca caacaaatcc ttgaaagcga cgccgaagat atccatagct gggtggaagg
    19501 caaactgatc gacaaagtgg gccagttagg caaaaagctg cataccgggc gtagccgtaa
    19561 tgatcaggta gcgactgacc tgaaactgtg gtgcaaagat accgttagcg agttactgac
    19621 ggctaaccgg cagctgcaat cggcgctggt ggaaaccgca caaaacaatc aggacgcggt
    19681 aatgccaggt tacactcacc tgcaacgcgc ccagccggtg acgttcgcgc actggtgcct
    19741 ggcctatgtt gagatgctgg cgcgtgatga aagccgtttg caggatgcgc ttaagcgtct
    19801 ggatgtcagc ccattaggct gtggcgcgct ggcgggaacg gcctatgaaa tcgaccgtga
    19861 acagttagca ggctggctgg gctttgcttc ggcgacccgt aacagtctgg acagcgtttc
    19921 tgaccgtgac catgtgttgg aactgctttc tgctgccgct atcggcatgg tgcatctgtc
    19981 gcgttttgct gaagatctga ttttctttaa caccggcgaa gcggggtttg tggagctttc
    20041 ggaccgcgtg acttccggtt catcattaat gccgcagaag aaaaacccgg atgcgctgga
    20101 gctgattcgc ggtaaatgcg gccgggtgca gggtgcgtta accggcatga tgatgacgct
    20161 gaaaggtttg ccgctggctt acaacaaaga tatgcaggaa gacaaagaag gtctgttcga
    20221 cgcgctcgat acctggctgg actgcctgca tatggcggcg ctggtactgg acggcattca
    20281 ggtgaaacgt ccgcgttgtc aggaagcggc gcagcagggg tatgcgaacg ccactgaact
    20341 ggcggattac ctggtggcga aaggcgtacc tttccgcgag gcgcaccata ttgtgggtga
    20401 agcggtggtg gaagccattc gtcagggcaa accgctggaa gatctgccac tcagtgagtt
    20461 gcagaaattc agtcaggtga ttggcgaaga tgtctatccg attctgtcgc tacaatcgtg
    20521 cctcgaaaag cgtgcggcaa aaggcggcgt ctcaccgcag caggtggcgc aggcgattgc
    20581 ttttgcgcag gctcggttag agtaatttgt ttatattgct cccctgaaaa tggggagttt
    20641 accacgccta caaagtgcga cacttcgtag gcggataagg cgtttacgcc gcatcaggca
    20701 gaaatttgtc agcattc
//
LOCUS       DADLZM010000066        20687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLZM010000066 DADLZM010000000
VERSION     DADLZM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.066"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNY73_002284"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNY73_002284"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY8048284.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JNY73_002285"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JNY73_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY8048285.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JNY73_002286"
     CDS             complement(2649..3200)
                     /locus_tag="JNY73_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY8048286.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JNY73_002287"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JNY73_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY8048287.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JNY73_002288"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JNY73_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY8048288.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNY73_002289"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNY73_002289"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY8048289.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNY73_002290"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNY73_002290"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY8048290.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNY73_002291"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNY73_002291"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY8048291.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLTKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKFIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNY73_002292"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNY73_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNY73_002293"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNY73_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY8048292.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JNY73_002294"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JNY73_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY8048293.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JNY73_002295"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JNY73_002295"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY8048294.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNY73_002296"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNY73_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY8048295.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNY73_002297"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNY73_002297"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY8048296.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNY73_002298"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNY73_002298"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY8048297.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSQTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNY73_002299"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNY73_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY8048298.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="JNY73_002300"
     CDS             complement(18174..18569)
                     /locus_tag="JNY73_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY8048299.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTEVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNY73_002301"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNY73_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8048300.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="JNY73_002302"
     CDS             19485..20078
                     /locus_tag="JNY73_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8048301.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /locus_tag="JNY73_002303"
     CDS             20223..20675
                     /locus_tag="JNY73_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY8048302.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4916 a         5209 c         5489 g         5073 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc ggatggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcactgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaaa catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gatgaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agtcagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt caggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggtggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtctgg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggaaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 acctccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaagcattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgcc
//
LOCUS       DADLZM010000067        20672 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLZM010000067 DADLZM010000000
VERSION     DADLZM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.067"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNY73_002304"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNY73_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY8048303.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNY73_002305"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNY73_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY8048304.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNY73_002306"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNY73_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY8048305.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIHEQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNY73_002307"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNY73_002307"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY8048306.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JNY73_002308"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JNY73_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY8048307.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JNY73_002309"
     CDS             complement(8217..9110)
                     /locus_tag="JNY73_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY8048308.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNY73_002310"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNY73_002310"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY8048309.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JNY73_002311"
     CDS             complement(10007..10189)
                     /locus_tag="JNY73_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY8048310.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JNY73_002312"
     CDS             complement(10241..11473)
                     /locus_tag="JNY73_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY8048311.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNY73_002313"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNY73_002313"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY8048312.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVVMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNY73_002314"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNY73_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY8048313.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16497)
                     /locus_tag="JNY73_002315"
                     /pseudo
     CDS             complement(14278..16497)
                     /locus_tag="JNY73_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNY73_002316"
     CDS             complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNY73_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY8048314.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNY73_002317"
     CDS             complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNY73_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY8048315.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNY73_002318"
     CDS             complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNY73_002318"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY8048316.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19788..20552)
                     /locus_tag="JNY73_002319"
     CDS             complement(19788..20552)
                     /locus_tag="JNY73_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8048317.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4968 a         5713 c         5019 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcaaacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgt ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc atattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaaacct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcaccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagaccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcaatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggctatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa
    15121 cgttcgccag gcgtttaatc accataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag
    15241 gattaacggc ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa
    15301 tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa
    15361 tatgacgatt tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga
    15421 aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca
    15481 aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac
    15541 aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg
    15601 tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg
    15661 aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg
    15721 tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc
    15781 agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc
    15841 agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa
    15901 accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata
    15961 ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag
    16021 cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc
    16081 atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg
    16141 aagttattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata
    16201 agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc
    16261 ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc
    16321 ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc
    16381 cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa
    16441 aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca
    16501 cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg
    16561 cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg
    16621 cgaggcagct tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct
    16681 cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta
    16741 ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga
    16801 ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca
    16861 atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct
    16921 tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac
    16981 ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga
    17041 ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca
    17101 agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta
    17161 gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta
    17221 acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt
    17281 gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga
    17341 accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca
    17401 acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt
    17461 acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt
    17521 ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt
    17581 tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca
    17641 gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg
    17701 atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc
    17761 cacgggttag ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata
    17821 tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt
    17881 ttgttggtcc aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca
    17941 acgaagcagc cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga
    18001 taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc cagggataca
    18061 cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg
    18121 atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca
    18181 acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact
    18241 tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg
    18301 ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta
    18361 aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga
    18421 gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg
    18481 ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag
    18541 gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact
    18601 gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt
    18661 ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg
    18721 tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct
    18781 tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat
    18841 atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc
    18901 attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt
    18961 attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag
    19021 catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt
    19081 tgatctcggc caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca
    19141 tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag
    19201 ggaataccac cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg
    19261 cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg
    19321 cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt
    19381 ccagattgcc attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg
    19441 catcttccga cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa
    19501 ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc
    19561 ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta
    19621 tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca
    19681 aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg
    19741 caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag
    19801 gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg
    19861 gactgggtgc gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg
    19921 gtgctgatgc cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta
    19981 tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa
    20041 agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa
    20101 gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga
    20161 acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag
    20221 ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc
    20281 gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg
    20341 ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc
    20401 gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata
    20461 gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc
    20521 gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat
    20581 aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc
    20641 acaaggtccg aaaaaaaacg ccgacaatgt gc
//
LOCUS       DADLZM010000068        20549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLZM010000068 DADLZM010000000
VERSION     DADLZM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.068"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JNY73_002320"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JNY73_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY8048318.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JNY73_002321"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JNY73_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY8048319.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JNY73_002322"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JNY73_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY8048320.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNY73_002323"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNY73_002323"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY8048321.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNY73_002324"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNY73_002324"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY8048322.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JNY73_002325"
     CDS             4449..4814
                     /locus_tag="JNY73_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY8048323.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JNY73_002326"
     CDS             4964..5482
                     /locus_tag="JNY73_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY8048324.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JNY73_002327"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JNY73_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JNY73_002328"
     CDS             6713..7195
                     /locus_tag="JNY73_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY8048325.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JNY73_002329"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JNY73_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY8048326.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JNY73_002330"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JNY73_002330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY8048327.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JNY73_002331"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JNY73_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY8048328.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JNY73_002332"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JNY73_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY8048329.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JNY73_002333"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JNY73_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY8048330.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JNY73_002334"
     CDS             10864..11730
                     /locus_tag="JNY73_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY8048331.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JNY73_002335"
     CDS             11751..13037
                     /locus_tag="JNY73_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY8048332.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JNY73_002336"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JNY73_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY8048333.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JNY73_002337"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JNY73_002337"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY8048334.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JNY73_002338"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JNY73_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY8048335.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JNY73_002339"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JNY73_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY8048336.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JNY73_002340"
     CDS             complement(16985..17275)
                     /locus_tag="JNY73_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY8048337.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JNY73_002341"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JNY73_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY8048338.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JNY73_002342"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JNY73_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY8048339.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JNY73_002343"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JNY73_002343"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JNY73_002344"
     CDS             19137..20378
                     /locus_tag="JNY73_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8048340.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAAARGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5364 c         4827 g         5056 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgt gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcggac agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgttgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaattagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcta
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttagacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgcat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatctc tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgccagtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggctcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg caggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ctgatttag
//
LOCUS       DADLZM010000069        20315 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLZM010000069 DADLZM010000000
VERSION     DADLZM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.069"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            140..292
                     /gene="hokA"
                     /locus_tag="JNY73_002345"
     CDS             140..292
                     /gene="hokA"
                     /locus_tag="JNY73_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY8048341.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(479..691)
                     /gene="cspA"
                     /locus_tag="JNY73_002346"
     CDS             complement(479..691)
                     /gene="cspA"
                     /locus_tag="JNY73_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY8048342.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(972..1262)
                     /locus_tag="JNY73_002347"
     CDS             complement(972..1262)
                     /locus_tag="JNY73_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY8048343.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1696..2406
                     /locus_tag="JNY73_002348"
     CDS             1696..2406
                     /locus_tag="JNY73_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY8048344.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2456..3430)
                     /gene="ghrB"
                     /locus_tag="JNY73_002349"
     CDS             complement(2456..3430)
                     /gene="ghrB"
                     /locus_tag="JNY73_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY8048345.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3534..4193)
                     /locus_tag="JNY73_002350"
     CDS             complement(3534..4193)
                     /locus_tag="JNY73_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY8048346.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4346..6679
                     /locus_tag="JNY73_002351"
     CDS             4346..6679
                     /locus_tag="JNY73_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY8048347.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6648..7088)
                     /locus_tag="JNY73_002352"
     CDS             complement(6648..7088)
                     /locus_tag="JNY73_002352"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8048348.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7085..7648)
                     /gene="tag"
                     /locus_tag="JNY73_002353"
     CDS             complement(7085..7648)
                     /gene="tag"
                     /locus_tag="JNY73_002353"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY8048349.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7806..8504
                     /locus_tag="JNY73_002354"
     CDS             7806..8504
                     /locus_tag="JNY73_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8048350.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDSTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8733..9941
                     /locus_tag="JNY73_002355"
     CDS             8733..9941
                     /locus_tag="JNY73_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048351.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10265..11956
                     /gene="eptB"
                     /locus_tag="JNY73_002356"
     CDS             10265..11956
                     /gene="eptB"
                     /locus_tag="JNY73_002356"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY8048352.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12048..12124
                     /locus_tag="JNY73_002357"
     tRNA            12048..12124
                     /locus_tag="JNY73_002357"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12082..12084,aa:Pro,seq:cgg)
     gene            12868..14475
                     /gene="dppA"
                     /locus_tag="JNY73_002358"
     CDS             12868..14475
                     /gene="dppA"
                     /locus_tag="JNY73_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY8048353.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14783..15802
                     /gene="dppB"
                     /locus_tag="JNY73_002359"
     CDS             14783..15802
                     /gene="dppB"
                     /locus_tag="JNY73_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY8048354.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15812..16714
                     /gene="dppC"
                     /locus_tag="JNY73_002360"
     CDS             15812..16714
                     /gene="dppC"
                     /locus_tag="JNY73_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY8048355.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16725..17708
                     /gene="dppD"
                     /locus_tag="JNY73_002361"
     CDS             16725..17708
                     /gene="dppD"
                     /locus_tag="JNY73_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY8048356.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17705..18709
                     /gene="dppF"
                     /locus_tag="JNY73_002362"
     CDS             17705..18709
                     /gene="dppF"
                     /locus_tag="JNY73_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY8048357.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18739..20010)
                     /locus_tag="JNY73_002363"
     CDS             complement(18739..20010)
                     /locus_tag="JNY73_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8048358.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4860 a         5029 c         5337 g         5089 t
ORIGIN      
        1 ttgaatatcg gaatcagacc tatatgcctc gtgtttaact gtccaacttt ttggggtcag
       61 tacaaagtga ggctacccta tgcctgaaca ccataaggtg tgcctcttac tcgttggaaa
      121 tcaacacagg gggctgggaa tgccgcagaa atatagatta ctttctttaa tagtgatttg
      181 tttcacgctt ttatttttca cctggatgat aagagattca ctgtgtgaat tgcatattaa
      241 acaggagagt tatgagctgg cggcgttttt agcctacaaa ttgaaagagt aagagtcttc
      301 ggcgggaaat tattcccgcc ttacttacgg cgttgcgcat tctcattgca cccacattta
      361 ttcttcacaa aaataataat agattttatt acgcgatcga ttatttattt cctgaaaaaa
      421 aataaaaaaa tccccgcaaa atggcaggga tcttagattc tgtgctttta agcagagatt
      481 acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca
      541 ctttctgacc ttcgtccaga gatttgtaac catagttctg gatagcagag aagtgtacga
      601 acacatcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc
      661 attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa ttaagtgtgc
      721 ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag
      781 gggtatcaac gataactctt gaagggactt gccttactac actgctttaa tggtctgtac
      841 gtcaaaccgt tgatgtgcat taagccactc attggcgggt gatgcaacaa ttatttttca
      901 tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag
      961 tggataaaag tctatttcat caactgctta cttaatgccg ggttggcttg aatcaaacgc
     1021 atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg
     1081 gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaatcctgt cccttttcgt
     1141 aaccgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca
     1201 tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc
     1261 attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat
     1321 agtcgggaaa agcagacaca gcggggttaa gtcagggcat gctctggcta tgattcgatt
     1381 cagcctgtca tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct
     1441 cttatcactc ctgttttgta aagatttcgc aaatctaacc ttctgcgaag gcgtaaatct
     1501 ctggctacaa aaaaggcaac ggcactatca ttttttaaca atgaaacaac aaattaacct
     1561 tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat cctgcaagcc
     1621 tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt
     1681 gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc gcctcttgcg
     1741 gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa
     1801 gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct accaaccctg
     1861 actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt
     1921 tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggtcgt agacagcgtt
     1981 aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaat
     2041 ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga agttgcaggc cgatgctgcg
     2101 cactctgcgc taaaacagag cgatgacctg aaaccggtat ttgatcaggc attcaccaaa
     2161 gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggcggcaca aactttcacg
     2221 caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt gagctttgtc
     2281 gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa actgattgcg
     2341 ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc
     2401 caataaagag taaaagcccg agcgattatc gggctttact tcacgcagtc gcggcttagt
     2461 ccgcgacgtg aggattcaca cagttcttct caacctttcc ttgtaacgca tcaatcaaat
     2521 tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat ccaatatgcg
     2581 gtactgcgac gacgttggcc attgagagca acggcgaatc tacggacagt ggctcttgtt
     2641 cgaagacatc cagcccggcg gcgtgaattt cgcctttctg caatgctgcg atcagtgcat
     2701 tttcgtcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg
     2761 cgaattgttc tgcgccaaac agatgatgcg tctcatcagt taacggcagg atcaggcaaa
     2821 cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg aagcgttctt
     2881 ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag ccaaagtgcg
     2941 cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt gttttatggt
     3001 gaacgtcagt gccgtaccag tccgggccta tgctcgcggt ccattcgcct gcttttaccc
     3061 gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc agcgtatcgg
     3121 cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg
     3181 catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc agtttcggca
     3241 ttttttccag caatgcggca tctacattct cgtttgaacc cagtaaacct tcagcttctg
     3301 caaaaattgc tgcattttga acgacggttt gtgggctgag gtttgccacc tggtgaacgg
     3361 tgaaatgcgc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg
     3421 acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg aaatatgatg
     3481 aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag ggattacagc
     3541 gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt gctgttgctg
     3601 gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg
     3661 gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat gttcaggtcg
     3721 tgaccccccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc
     3781 agcaccattg ccacgccggt cagggtgtta gcgcctgccg gtttcagggg cgcgctgctg
     3841 ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg
     3901 ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa
     3961 cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa tcagcgcgcc tttaccgcga
     4021 tctttcttcg aagaagagag cgcaccaata cccgcgccca cgagagagcc cagacctgcg
     4081 ccgatagcag atttgcctgc ttcgcgttcg ccggtgtaag ggttagttgt gcagccagat
     4141 accgccagag cgccactcac tacggcggca ataagataaa cacgtttctt cattgttaat
     4201 ccttaataac ctttttattc tttgccacgg gttccgtggc gggagattat gccgcgtgaa
     4261 catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac
     4321 atctaacctt tcaggagtct tcggtttggc caactcatcc tcacgatatt ccgttctgac
     4381 tgccgcccac tgggggccca tgctggttga aaccgacggc gaaaccgtgt ttagctcgcg
     4441 tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca
     4501 cagcaatacg cgggtacgat ttccaatggt gcgcaaaggc tttctcgcgt caccggaaaa
     4561 cccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga
     4621 tcttattcac caacaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc
     4681 tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg
     4741 ctatatggcg ctggcaggcg gttataccgg gcatctgggg gattattcga ccggtgcggc
     4801 acaggcgatc atgccgtatg tcgtaggtgg cagcgaagtt tatcaacagc aaaccagttg
     4861 gccgctggtg ctggaacata gcgatgtcgt ggtgttgtgg agtgctaacc cactcaatac
     4921 gctgaaaatt gcgtggaatg catccgatga gcaggggctt tcttactttt ccgcactgcg
     4981 tgacagtggg aaaaagctga tctgcattga tccaatgcga tcggaaaccg tcgatttctt
     5041 tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgc tgatgctggg
     5101 gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg cgcattgcac
     5161 cacaggttat gccgtcttcg cctcttattt gctgggcgag agtgacggaa tagcgaaaaa
     5221 cgccgaatgg gcggcagaga tttgtggtgt tggcgcagcg aaaatccgcg agctggcggc
     5281 tattttccac caaaatacca ccatgctgat ggcaggctgg ggaatgcagc gccaacagtt
     5341 tggtgagcaa aaacactgga tgatcgtcac gctggcagca atgttggggc aaatcggcac
     5401 acccggcggc ggttttggtc tttcttacca ttttgccaat ggtggtaacc ccacgcggcg
     5461 ttctgcggtg ctctcttcca tgcagggcag cttgccgggt ggctgcgatg cggtggataa
     5521 aatccctgtt gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat atcaacacaa
     5581 cggtatgaac cgacatttcc cggatattcg ttttatctgg tgggcgggcg gtgccaactt
     5641 tactcatcat caggatacca atcgtctgat ccgtgcctgg caaaaaccgg agctggtggt
     5701 gatctctgaa tgcttctgga cggcggcggc gaaacacgcg gatatcgttc tgcctgcgac
     5761 tacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc agcatctggt
     5821 gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt aatgattttg atgtttttgc
     5881 cgagttgagt gaacgttggg agaagggcgg ctatgcgcgg tttacggaag gaaaaagtca
     5941 gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa gccagcaggt
     6001 tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga tgccggaaaa
     6061 cccggacagc gagcggttta ttcgcttcgc tgatttttgc cgcgatccgc tggcgcatcc
     6121 gttaaaaacc gccagcggca agattgaaat cttctcacag cgtattgccg attacggtta
     6181 cccggattgc cctgggcatc caatgtggct ggagccggac gaatggcagg gcaatgccga
     6241 accagaacag ttgcaggtac tttctgccca tccggcgcac cgcctgcaca gccagctgaa
     6301 ttacagttct ctgcgcgaat tgtacgcggt agcaaaccgt gagcctgtca ccattcatcc
     6361 tgacgatgct caggcgcgcg gcataaccga aggggatatg gtgcgggtgt ggaactcccg
     6421 tgggcagatc cttgctggag cggtcattag cgagggaatt aaacctggcg tgatttgcat
     6481 tcacgaaggg gcatggccgg atctggattt aaccgctgac ggtatttgta aaaacggcgc
     6541 agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa
     6601 tacggcgctg gcatggctgg aaaaatacaa cggtccggaa ctgacactta cagcgtttga
     6661 accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcaatcg
     6721 acaatgtgaa aaccctgcgc acggtaaaaa tctatcgccg gttgattttt ttgataaacc
     6781 tccagcatca gatggggata gcgctgctgc acatactgca tcagcgcctt accaataccg
     6841 cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc cataatgctg
     6901 acaaaaccga gaagcttacc gtcctcttcc cagacccagt tttgcgcgtt ggcaagatag
     6961 gcatcccgca ccagcggaat gcagtcacgc cagtaattcg cttttataaa gggatgcccc
     7021 caggttgtac tttccagcca cagttcgagg atcgcgggga gttctgaacg ttgcgcttcc
     7081 cgaatcatgg tttatttccc ggatagcaac agcagccaac cacatgatca ttcaccagcc
     7141 cacatgcctg cataaaggag taacagattg tggtgccgac aaacttaaaa ccacattttt
     7201 tcagtgcctt agatagggcg tcggaggcgg acgtagatgt gggaatttcg ctcaacgttg
     7261 tggcttgtgt cacctgtggc tgatgattta caaacgacca gacaaagtcg ggaaacggtt
     7321 cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc
     7381 ctcgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg
     7441 ctgcgacctt caccggatcg aactgatgaa agcaggcgcg atagttttcg cgttttttga
     7501 ggacggtgat ccacgataac ccagcctgct gcccttcaag gcagatcatt tcgaacagtt
     7561 ttttactgtc agtttcaggc acgccccact cattatcatg gtaggcaata taaagcgggc
     7621 cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa
     7681 tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat
     7741 gcccgatatc cttcatacaa tgacaataat attgccgaat tcggctcttc cctggagtcg
     7801 tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctg gcgagcgtgg
     7861 taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata
     7921 cgcaacccgg acattccaca gagcgttaca cctgggatag tgatcatcaa cctgattaca
     7981 acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc
     8041 tggcggaaga aactcctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt
     8101 tatatgaaca ggttacaacc ggcccggtcg cggcattaca ttacgatagc acaaccacct
     8161 cgatgtataa cgagtttggc gacagtacta ccacgctgac cgatccgtta tggcatgcca
     8221 gcgtgagtag cttaggctgg cgtgttgact cccggcttgg cgatctccga ccctgggcgc
     8281 aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc
     8341 ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt
     8401 tgctcaatca aaatattgcc gcctatgctg cgctaactca ggcagaaaat accactaata
     8461 atagcgacta tctgtatacg atgggggtta gcgccagatt ttaacgtaac agtcacaatt
     8521 gaaaccattg aataacaaca gttgtggcga tagtgggtgc taacttacca aataataaat
     8581 ttggtgaata attgtcgcgt cattcattcc tgaactaagg catttcattc cgttctgatg
     8641 gcatttcatg ccgtttttcc ccaagcataa aatgcacttc gttatggttg tcggcagaga
     8701 tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat cagcgtaccc
     8761 gctggctgac actcatcggt actatcatta cccagtttgc gctggggtcg gtttatacct
     8821 ggagcctgtt taatggcgcg ctttctgcca agctggatgc gccggtaagc caggtcgctt
     8881 tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg ggcaaattgc
     8941 aggaacgttt tggcgttaaa cgcgtcaccg tggcttccgg cattttgctg ggattaggct
     9001 tcttcctgac cgcgcattcc aacaacctga tgatgctgtg gttaagcgcc ggtgtgctgg
     9061 tcggtctggc ggatggcgcg ggttacctgc tgacgctctc taactgcgtg aagtggttcc
     9121 cggagcgtaa aggtctgatc tccgcgttcg ctatcggttc ttatggtctg ggtagcctgg
     9181 gtttcaaatt tatcgacacg cagctgctcg aaacggtggg tctggaaaaa acctttgtga
     9241 tttggggagc gattgcgctg gtgatgattg ttttcggcgc aacgttaatg aaagacgcac
     9301 caaaacagga agtgaaaacc agcaatggtg tggtggagaa agattacacg ctggcagagt
     9361 cgatgcgtaa accgcagtac tggatgttag cggtaatgtt cctgactgcc tgcatgagcg
     9421 gcctgtacgt gattggggta gcgaaagata tcgcccaaag tctggcacac cttgatgtgg
     9481 tttccgcagc caatgcagta acggttattt ccatcgctaa cctttcaggt cgtctggtgc
     9541 tgggcattct gtctgacaaa attgcccgta tccgtgttat caccattggt caggtgattt
     9601 cgctggtggg tatggcggcc ctgctgtttg caccattaaa tgcagtgacg ttctttgcag
     9661 cgattgcctg cgtggcattt aactttggcg gcactattac cgtctttcca tcactggtca
     9721 gtgagttctt cggcctcaat aacctggcga aaaactacgg tgtgatttac ctcggtttcg
     9781 gtatcggtag catttttggt tcgattatcg cctcactgtt tggcggtttc tatgtgacct
     9841 tctacgtgat tttcgccctg ctgattctgt cattggcgct ttctacgacg attcgtcagc
     9901 cagagcagaa aatgttgcgt gaggcgcatg gctcccttta atgctcagcc gcgagctact
     9961 gggcttcagc ttccgtgtgc atcaaacgca tcaacgaaat gtataaacaa tgactcgtaa
    10021 aatttgggcc agttaactgg ccctttttac tgctttttgt aacgctaaag tctcttttca
    10081 aacttgcatt tttgtaaatt tgtgcttcat gcacactctt tccccacact ttttcgcttt
    10141 gctgtggtct acttattcgc gcgtgtagat tttacttatc tgactacctc cgcacttttc
    10201 ccctgccggg cctgaaaagc cgctaagcag ggtgttatca cctgtttgtc cagggtttgt
    10261 ttgcatgaga tacatcaaat cgattacaca gcagaagctg agctttttgc ttgcaatcta
    10321 tattggcctt tttatgaatg gcgcggtttt ttatcgccgc ttcggcagct atgcgcacga
    10381 ttttaccgtc tggaaaggcg tttctgctgt tgttgaactg gccgccaccg tactggtgac
    10441 cttcttttta ctacgtcttc tatcgctgtt tggccgccgc agctggcgta ttctggcgtc
    10501 gctggtggtg ctcttttccg caggtgccag ctattacatg accttcctta atgtggtcat
    10561 tggttatggc atcatcgctt ccgtcatgac caccgatatc gacctgtcaa aagaagttgt
    10621 tggtctgaac tttattctct ggttaatcgc cgttagtgca ttgcctctta tccttatctg
    10681 gaataaccgc tgtcgctaca ccttgctccg acaactgcga accccagggc agcgtattcg
    10741 cagcctggtg gtcgtcgtac tggcgggtat tatggtttgg gcaccgattc gtttgctgga
    10801 tatccagcag aagaaagtgg agagggcgac cggcgttgat ttgccgagtt atggcggtgt
    10861 cgtagcgaac tcttatctgc catcaaactg gctttctgcg ttggggctgt atgcctgggc
    10921 gcgggtcgat gaatcttccg ataataattc attgcttaat ccggcgaaga agtttacata
    10981 ccaggctcca caaaacgttg atgatactta tgtcgtgttt atcatcggtg aaaccacgcg
    11041 ttgggaccat atgggtattt tcggctatga gcgtaatacc acgccgaaac tggcccagga
    11101 gaaaaatctg gcggcgttcc gtggttactc ctgtgatacc gcaaccaaac tctcactgcg
    11161 ttgcatgttt gtacgtcagg ggggcgcgga agataatccg cagcgcacat taaaagaaca
    11221 gaacattttc gcggttctga agcagttagg attcagttct gacctctacg ctatgcagag
    11281 cgaaatgtgg ttctacagca acacgatggc agacaacatt gcttatcgtg agcagattgg
    11341 tgcggagcca cgtaatcgtg gcaagccggt agatgatatg ttgctggtag acgaaatgca
    11401 gcaatcgcta gggcgcaacc cggatggtaa gcatctgatc attctgcata ccaaaggttc
    11461 gcattttaac tacacccagc gttatccgcg tagcttcgcg cagtggaagc cggaatgtat
    11521 tggtgttgat agcggctgta ccaaagcgca gatgatcaac tcctatgaca actcggtgac
    11581 ctatgtggat cactttatct ccagcgtgat tgatcaggtt cgcgataaga aagcgattgt
    11641 gttctacgca gctgaccacg gtgagtcaat taatgaacgc gagcacctgc acggcacgcc
    11701 gcgtgaactg gcaccgccgg agcagttccg cgtaccgatg atggtctgga tgtcagataa
    11761 atatctggaa aatccggcca atgcgcaggc gtttgcgcag ctgaaaaaag aagccgacat
    11821 gaaagtgcca cgccgtcacg tagagctgta cgacaccatc atgggttgtc ttggctatac
    11881 ttcaccggat ggtggaatta acgaaaacaa caactggtgt cacatcccgc agacaaaagt
    11941 ggcagcggct aactaagcgg cctgctgact ttctcgccga tcaaaaggca ttttgctatt
    12001 aagggattga cgagggcgta tctgcgcagt aagatgcgct ccgcattcgg tgattggcgc
    12061 agcctggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc tctatcaccg
    12121 accaaattcg aaaagcctgc tcagcgagca ggtttttttg catttgtagc tcatgaggat
    12181 gagaacctcc gggggcaggg aggctcgact cgacgtaggc ctgataagac gcgccagcgt
    12241 cgcatcaggc aacggcaccg cacttattgt cggatgcggc gcgaccgcct tattcgacca
    12301 acacaccaca cattttcctg ccaactttgc gccccatatc tcactatcca ttaacggatt
    12361 tgtgacagaa accattgtct ttttttatat ttgcttaaat cttttttgcg ttacttatgg
    12421 ctggcgctgg cattttccgc tgtgcgtttt agcgttcatg gtattaatcg ctcagatatc
    12481 caccttttcc tcagattttt ttcattaaat gcataaaact taatcaccaa attttgctaa
    12541 atattaagct gattgttctg gttatgttgg agtagcacaa atttctctgc tgcaaatagc
    12601 tgtttgaagt ttttttaatc tttgtttgtg gattctcacc gttggtgtaa atcccggttg
    12661 gctgtattga cgttttcaca ttctgttgac agattgtagg tcacgagggg cattttatgg
    12721 aggatccgca ctgttacact gatgttaatt agtacggcat ccccacctca taacgttgac
    12781 ccgaccgggc aaaaaacaaa aaaggtcagg cagcgacaac ccactgcaaa gggttaaaac
    12841 aacaaacatc acaattggag cagaataatg cgtatttcct tgaaaaagtc agggatgctg
    12901 aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa gtgttcaggc taaaactctg
    12961 gtttattgct cagaaggatc tccggaaggg tttaacccgc agctgtttac ctccggcacc
    13021 acctatgacg cctcttccgt cccgctttat aaccgcttgg ttgaatttaa aatcggcacc
    13081 accgaagtga tcccgggcct cgctgaaaag tgggaagtca gcgaagacgg taaaacctat
    13141 accttccatc tgcgtaaagg tgtgaagtgg cacgacaata aagaattcaa accgacgcgc
    13201 gaaatgaacg ccgatgacgt ggtgttctcg ttcgatcgtc agaaaaacgc gcaaaacccg
    13261 taccataaag tttctggcgg cagctacgaa tacttcgaag gtatgggctt gccggagctg
    13321 atcagcgaag tgaaaaaggt ggacgataac accgttcagt ttgtgctgac tcgaccggaa
    13381 gcgccgttcc tcgctgacct ggcaatggac ttcgcctcta ttctgtcaaa agaatatgct
    13441 gacgcgatga tgaaagccgg tacaccggaa aaactggacc tcaacccaat cggaaccggt
    13501 ccgttccagt tacagcagta ccaaaaagat tcccgtatcc gctacaaagc gtttgatggc
    13561 tactggggca ccaaaccgca gatcgatacg ctggtcttct ctattacccc tgatgcttcc
    13621 gtgcgttacg cgaaattgca gaagaatgaa tgccaggtga tgccgtaccc gaacccggca
    13681 gacatcgctc gcatgaagca ggataaatcc atcaacctga tggaaatgcc ggggctgaac
    13741 gtcggctacc tctcgtataa cgtgcagaaa aaaccgctgg atgacgtgaa agttcgccag
    13801 gcactgacct acgcggtgaa caaagacgcg atcatcaaag cggtttatca gggcgcgggt
    13861 gtatcagcga aaaacctgat cccgccaacc atgtggggct ataacgacga cgttcaggat
    13921 tacacctacg atcctgaaaa agcgaaagcc ttgctgaaag aagcgggtct ggaaaaaggt
    13981 ttctccatcg acctgtgggc gatgccggta caacgtccgt ataacccgaa cgctcgccgc
    14041 atggcggaga tgattcaggc agactgggca aaagtgggcg tgcaggccaa aatcgtcacc
    14101 tacgaatggg gtgagtacct caagcgtgcg aaagatggcg aacatcagac ggtaatgatg
    14161 ggctggactg gcgataacgg ggacccggat aacttcttcg ccaccctgtt cagctgcgcc
    14221 gcctctgaac aaggttctaa ctactcaaaa tggtgctaca aaccgtttga agatctgatt
    14281 caaccggcgc gtgctaccga cgaccataac aaacgcgttg aactgtacaa gcaagcgcag
    14341 gtggtgatgc acgatcaggc tccggcactg atcatcgctc actccaccgt gtttgaaccg
    14401 gtacgcaaag aagtcaaagg ctatgtggtt gatccattag gcaaacatca cttcgaaaac
    14461 gtctctatcg aataattaaa agccatacaa gactgatggc aaaggcaaaa ctgcctgatg
    14521 tgctccgctt atcaggccta cgaaaattct gcaatgtatt gaatttgcac gattttgtag
    14581 gccggataag gcgttcacgc cgcatccggc ataaacaaag cgcactttgt caacaatctg
    14641 tatacccggt ggcgctgtgc ctctgcgtgc agcgccatcc ggcagcaata cttcattctc
    14701 tgtccgacaa gggcgggaat tgatttgtga gcaatacaga cacgcagttc caggctgcgg
    14761 gtcactatag agaatccggg ttatgttgca gtttattctc cgacgtttgg gactcgtcat
    14821 ccccacgttt atcggtatta cccttctcac atttgccttt gtccacatga tcccgggcga
    14881 tccggtgatg atcatggcag gcgaacgtgg gatctcccct gagcgtcacg tgcagctgct
    14941 ggctgaactc ggcttagata aaccgatgtg gcagcagtat ctccattaca tttggggcgt
    15001 aatgcacggc gatctaggca tttcaatgaa aagccgaatt ccagtatggg aagagttcgt
    15061 gccgcgcttt caggccacgc tggaacttgg cgtctgcgcg atgatttttg ctacggcagt
    15121 cggtattccg gttggtgtgc tggctgcggt taaacgcggt tccattttcg atcacactgc
    15181 agttggcctg gcgctgaccg gctactcgat gccgatcttc tggtggggca tgatgctgat
    15241 catgctggtt tcggtgcact ggaacctgac gcccgtctcc ggtcgcgtga gcgatatggt
    15301 gttcctcgat gactccaatc cgttaaccgg ttttatgcta atcgacaccg ccatctgggg
    15361 tgaagacggc aactttatcg atgccgtcgc ccatatgatc ttgcccgcca ttgtgctggg
    15421 tactattccg ctggcggtca ttgtgcgtat gacacgctcc tcgatgctgg aagtgctggg
    15481 cgaggattac atccgcaccg cgcgcgccaa agggctaacc cgcatgcggg tgattatcgt
    15541 ccatgcgctg cgtaacgcga tgctgccggt agtgaccgtt atcggcctcc aggtgggaac
    15601 attgctggcg ggggcgattc tgaccgaaac catcttctcg tggcccggtc tggggcgctg
    15661 gttgattgac gcactgcaac gccgcgacta tccggtagtg cagggcggcg tattgctggt
    15721 ggcgacgatg attatcctcg tcaacttgct ggtcgatctg ctgtacggcg tggtgaaccc
    15781 gcgtattcgt cataagaagt aaggggacat catgtcacag gttactgaaa ataaagtgat
    15841 tagcgcaccg gtgccgatga ccccgttaca ggagttctgg cactatttta aacgcaacaa
    15901 aggcgcggtc gtcgggctgg tttacgtcgc catcgtgctg ttcatcgcga tctttgccaa
    15961 ctggattgcg ccttataacc cggcggatca gttccgcgat gcgctgctcg ccccgccagc
    16021 ctggcaggaa ggcggcagta tggcacactt gctgggcacc gatgacgtcg gtcgtgatgt
    16081 gctgtcgcgg ctgatgtacg gcgcgcgttt atcgctgctg gttggctgtc tggtggtggt
    16141 gttgtcgctg attatgggcg ttattctcgg cctgatcgcc ggttacttcg gcggcctggt
    16201 tgataacatt atcatgcgcg tggtcgatat catgctggcg ctgccaagtc tgctgttggc
    16261 actggtgctg gtggcgattt tcggcccgtc gattggtaac gccgcgctgg cgctgacctt
    16321 cgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc gtgctggtgg aggtgaatcg
    16381 cgattacgtc accgcctctc gcgtggcagg tgccggggcg atgcgccaga tgtttattaa
    16441 catcttcccg aactgccttg cgccgctgat tgttcaggcg tcgctcggtt tctctaacgc
    16501 cattctcgat atggctgctc ttggtttcct cggcatgggg gcacagccgc caacgcctga
    16561 gtggggcacc atgctctccg acgtgttgca gttcgcgcaa agcgcctggt gggtcgtgac
    16621 cttccctggt ctggcgatcc tgttgacggt gctggcattt aacctgatgg gtgacggtct
    16681 gcgtgacgcg ctcgatccca aactgaagca gtaagaggtt cgagatggcg ttattaaatg
    16741 tagataaatt atcggtgcat ttcggcgacg aaagcgcacc gttccgcgcc gtagaccgca
    16801 tcagctacag cgtaaaacag ggcgaagtgg tcgggattgt gggtgagtcc ggctccggta
    16861 agtcggtcag ttcactggcg attatggggc tgattgatta tccgggccgc gtaatggcag
    16921 aaaaactgga gtttaacggc caggatttgc agcgtatctc agaaaaagag cgccgcaacc
    16981 tggtgggtgc cgaagtggcg atgatcttcc aggacccgat gaccagcctt aacccatgct
    17041 acaccgtggg tttccagatt atggaagcga ttaaagtgca tcagggcggc aacaaaagta
    17101 cccgccgtca gcgggcgatt gacctgctga atcaggtcgg tattcccgat ccggcttcgc
    17161 gtctggatgt ttacccgcat cagctttccg gcggcatgag ccagcgcgtg atgatcgcca
    17221 tggcgattgc ctgtcggcca aaactgctga ttgccgatga accgaccacc gcgctggacg
    17281 tgaccattca ggcgcaaatc atcgaactac tgctggagct acagcagaaa gagaacatgg
    17341 cgctggtgtt aattacccat gacctggcgc tggtggcgga agctgcgcat aaaatcattg
    17401 tgatgtatgc aggtcaggta gtagaaacgg gcgatgcaca cgccatcttc catgcgccgc
    17461 gtcacccgta tactcaggca ttgctgcgtg cgctgccaga atttgctcag gacaaagaac
    17521 gtctggcgtc gttgccaggt gttgttcccg gcaagtatga ccgcccgaac ggctgcctgc
    17581 ttaacccgcg ctgcccctat gccactgaca gatgtcgcgc tgaagaaccg gcgctgaata
    17641 tgctcgctga tgggcgtcag tccaaatgcc attacccact tgatgatgcc gggaggccca
    17701 cactatgagt acgcaagagg ccacctcgca acaaccgctg ttgcaggcta tcgacctgaa
    17761 aaaacattat ccggtgaaga aaggcatgtt cgtgccggaa cgtctggtga aggcgctgga
    17821 cggcgtttcg tttaaccttg aacgtggcaa aacgctggca gtagtgggcg aatctggctg
    17881 cggtaaatcg accctcggtc ggttgctgac gatgatcgaa acgcccaccg gcggcgagct
    17941 gtattatcag gggcaggatc tgctcaagca cgatccgcag gcgcagaagc tgcgtcggca
    18001 gaaaatccag atcgtcttcc agaatcccta cggttcgctg aatccgcgta agaaagttgg
    18061 gcaaattctc gaagagccgc tgcttattaa caccagctta agcaaagaac agcgtcggga
    18121 aaaagccctg tcgatgatgg caaaagtcgg cctgaaaacc gagcactatg accgctatcc
    18181 gcacatgttt tccggcggac aacgtcagcg tatcgctatt gcccgtggtc tgatgctcga
    18241 cccggatgtg gtgattgccg atgagccggt ttctgcgctg gacgtctcag tgcgtgcgca
    18301 ggtgctgaat ctgatgatgg atttgcagca ggagttgggg ctttcttatg tctttatctc
    18361 ccacgacctg tcggttgtgg agcacattgc tgatgaagtg atggtgatgt acctaggccg
    18421 ctgcgtggag aaaggaacga aggaccaaat cttcaataac ccgcgccatc cgtacaccca
    18481 ggcgctgctc tccgcgacgc cacgcctgaa cccggacgat cgtcgtgaac gcatcaaact
    18541 caccggtgaa ctgccaagcc cgctgaatcc tccgccgggt tgcgccttca acgcccgctg
    18601 tcgtcggcgc ttcggcccct gcacccagtt gcagccgcag ctaaaagact acggaggtca
    18661 actggtagct tgttttgctg ttgatcagga tgaaaatccg cagcgttaac tcacaaaacc
    18721 ggggccatgc gctccggttt actcaatcag cttcaacagt ggcgacaagc atagcaaaat
    18781 gccgtaaagc agaatcagcc aggctttaaa tccgcgaagt ttttccagtt gtgcgacttt
    18841 atagatcagg aaaaacggaa tgaggcacga cacaatgcca tataacgggc ttcccaactg
    18901 aaagaacacc agcactgata cacgaaacga aacccaaatc gtcaacgtaa tgacgataaa
    18961 agcgcagatc gccagagtca gcatgcgtga gttaattttc ttggtatcaa taattcggct
    19021 taacagattg agaataatgc ctttaatagc ctcgtggaaa ccgagataaa tgccaaagaa
    19081 tgcggtcagt acggcaaaga tattcagcac cgtagaggtg atatgaatga tatgtccagg
    19141 gatcacctgc gcggccagtg ccagtgccga gatattttgt tcaaaggcag aaacggcttc
    19201 ttcgtggcta attgagaagg taaacgaaaa ggcaaaaaac aggatcaccg cgatgagcgt
    19261 gatataacta attcggtggg tacgcagcgc gagccgggtt gccagtactt tatctgcttc
    19321 ccgtttacga taggcaatat tcattgggtt aagtacctga ataaataccg cagaaaagaa
    19381 gcaaaatgga atggtaagca ggacatcgcg gaaaaagacg gaggcttgcg ggaaggcggt
    19441 tatattggcg aaattccagt gcgggatcat cgcaaaaccg aacacgacaa taatccctac
    19501 tttgaccacc accattggcc cagaaatctt aaacagtaat cgttcaccac cagacgcaat
    19561 cgctatcagt acggcgaaaa tagcgacttt ataaagtaga gattgtgaaa gatcggcatc
    19621 ggttaaaccg aaggttttca ggtacgaggc gctgtcgaaa accacggaga gagagtagat
    19681 aaaaatcccg tggataatca tcaaaaagta gataaccccg aggaaaattc cccagttctt
    19741 ccccaggtaa tgactgataa tatcggtgta gtcattacag gaatcgcttt cagaaagggt
    19801 ttttaaataa atgtcctgca ctacccaggt ggcaggataa gcaatgatcg ctgcggtaat
    19861 aaatacccaa attcccttca agccaatttg tactggcatc agcacggttc cggcaccaat
    19921 cgccatgcct atgcataata aaacccagcc gaagtcgtag cgtgtaaacg gcaatttctg
    19981 attgtgtttc gatagtgtgt tgtgctgcat aaagtcctgt ccagaaagaa gtcggcctcg
    20041 caaaaatgaa taaacccagt ttctcttgcg caacaggtgg agaagtcgaa tacgcagtat
    20101 gtaaaaaatg aggcaacaga ttcactgggg aggtcgcgag gaggcgcaat tctacaaaat
    20161 tgcgcatttc actaccagtc tggagtcagc ccggatatga actgcaacta ttgcaaataa
    20221 agaaattaag ctggaaggca tcgcgcaatg aggaagattg ccgggtgctg acagtaaaaa
    20281 gcaatcgtaa aatcagcggc aagccggaag ggttc
//
LOCUS       DADLZM010000070        19916 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLZM010000070 DADLZM010000000
VERSION     DADLZM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.070"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..744)
                     /locus_tag="JNY73_002364"
     CDS             complement(<1..744)
                     /locus_tag="JNY73_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8048359.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            838..1035
                     /locus_tag="JNY73_002365"
     CDS             838..1035
                     /locus_tag="JNY73_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8048360.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGTHIENDDRIN"
     gene            1129..1317
                     /locus_tag="JNY73_002366"
     CDS             1129..1317
                     /locus_tag="JNY73_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8048361.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1238..1963
                     /locus_tag="JNY73_002367"
     CDS             1238..1963
                     /locus_tag="JNY73_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HAY8048362.1"
                     /translation="MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVET
                     ISCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETD
                     NRGYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQ
                     ASNFDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYI
                     RFLNKRARKRLNTKLFKVQPYPK"
     gene            complement(2075..2422)
                     /locus_tag="JNY73_002368"
                     /pseudo
     CDS             complement(2075..2422)
                     /locus_tag="JNY73_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="JNY73_002369"
                     /pseudo
     CDS             complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="JNY73_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4429..5487
                     /gene="citC"
                     /locus_tag="JNY73_002370"
     CDS             4429..5487
                     /gene="citC"
                     /locus_tag="JNY73_002370"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY8048363.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5502..5798
                     /gene="citD"
                     /locus_tag="JNY73_002371"
     CDS             5502..5798
                     /gene="citD"
                     /locus_tag="JNY73_002371"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY8048364.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5795..6703
                     /gene="citE"
                     /locus_tag="JNY73_002372"
     CDS             5795..6703
                     /gene="citE"
                     /locus_tag="JNY73_002372"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY8048365.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6714..8246
                     /gene="citF"
                     /locus_tag="JNY73_002373"
     CDS             6714..8246
                     /gene="citF"
                     /locus_tag="JNY73_002373"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY8048366.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8250..8801
                     /gene="citX"
                     /locus_tag="JNY73_002374"
     CDS             8250..8801
                     /gene="citX"
                     /locus_tag="JNY73_002374"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY8048367.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8776..9654
                     /gene="citG"
                     /locus_tag="JNY73_002375"
     CDS             8776..9654
                     /gene="citG"
                     /locus_tag="JNY73_002375"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY8048368.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9705..11168
                     /gene="citT"
                     /locus_tag="JNY73_002376"
     CDS             9705..11168
                     /gene="citT"
                     /locus_tag="JNY73_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY8048369.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11282..12088
                     /gene="rna"
                     /locus_tag="JNY73_002377"
     CDS             11282..12088
                     /gene="rna"
                     /locus_tag="JNY73_002377"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY8048370.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12317..12727
                     /gene="rnk"
                     /locus_tag="JNY73_002378"
     CDS             12317..12727
                     /gene="rnk"
                     /locus_tag="JNY73_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY8048371.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12958..14195)
                     /locus_tag="JNY73_002379"
                     /pseudo
     CDS             complement(12958..14195)
                     /locus_tag="JNY73_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14416..14844
                     /gene="uspG"
                     /locus_tag="JNY73_002380"
     CDS             14416..14844
                     /gene="uspG"
                     /locus_tag="JNY73_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY8048372.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="JNY73_002381"
     CDS             complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="JNY73_002381"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY8048373.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="JNY73_002382"
     CDS             complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="JNY73_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY8048374.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17710..18456
                     /gene="dsbG"
                     /locus_tag="JNY73_002383"
     CDS             17710..18456
                     /gene="dsbG"
                     /locus_tag="JNY73_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY8048375.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18666..19568
                     /locus_tag="JNY73_002384"
     CDS             18666..19568
                     /locus_tag="JNY73_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8048376.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19715..19916
                     /locus_tag="JNY73_002385"
                     /pseudo
     CDS             19715..19916
                     /locus_tag="JNY73_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5018 a         5128 c         4758 g         5012 t
ORIGIN      
        1 aacttgcgaa ccgcaaagcc agttcccgtt attatccatg tacgcctgac catcggtgcc
       61 attggctgtc cttgagttat taatcatgta gatgccaaat tgcctattcc ccagcccacc
      121 aattacgaat ttacggtcgg tatggtcctg gcgaagcaat gcctgcgcac catcgttgga
      181 taccgcatta cggcccataa taacgttctg gtcacgcata tgaatccaca tgcctgtact
      241 gctgttaatt aaaaaacggt ttgtaaatat atctcctgta acaaccagac catgccccat
      301 gcttatccgg ccagttctga gattaagcgt aaaggggcgt agtggcccta tattaccatt
      361 ttccccttca ttctctcgtg tagggatgat atgcaggcat tcttcagaac gacgaaaaat
      421 ggcaccaaaa gatgaattaa atattctcag tgcattgacc gtcgatattt ttacttcact
      481 gctgaaaagg gctttaacaa gaacatacag agcatcccat ttaagattca tcaggtcttt
      541 aacccggccg ccagtgaggt ggtgtctcca tttgaaatac tcattgccgt tgtcgcctgt
      601 ttcaaaccac atgtatgaat cagtatcgct gtcggcatca tttttaaatc caatctttgc
      661 ccagtcagta ttccgaatcc aggcaaggat tgagtcgttt tcaaaagtaa gtccaccgga
      721 caacgtatcg ccattttttt gcacggcgtt accagccttg tttaccgttt cctgtaaacc
      781 gaggggccgt ttctggcctg catggcatga tttccgcttt taaacgggga attcagcgtg
      841 ctgattggct atgtaagggt atcaacaaat gaccagaata cagacctgca acgaaacgct
      901 cttgtttgtg caggatgtga acaaatattt gaagataaat taagcggaac aaggacagac
      961 cgaccgggat taaaacgtgc tttaaagcgc cttcaaaaag gaacgcatat agaaaacgac
     1021 gatcgaatca attaaatcga tctatattac cgatcgatta tgtcccgata accacacccg
     1081 actgatgaga ttttttaaga taacggcgaa ttgatgcaag gaggtgaaat gaaatcaatt
     1141 cgctgtaaga actgcaacaa actgttattt aaagcggatt cctttgatca cattgaaatc
     1201 aggtgtccgc gttgcaaacg tcacatcata atgctgaatg cctgcgagca tcccacggag
     1261 aaacattgtg ggaaaagaga agaaatcacg cattctgacg aaaccgtgcg ttattgagta
     1321 tgaaggccag attgttggct atggttcaaa ggagctgcgc gttgaaacca tatcctgctg
     1381 gctggcccgc acaatcattc agacaaagca ctattcccgc cgttttgtga ataactctta
     1441 ccttcacctg ggagtattca gcgggcgtga tctggttggc gttctccagt ggggatatgc
     1501 ccttaacccc aactcaggtc gtcgtgtcgt gcttgaaacg gataaccggg gctatatgga
     1561 gttgaatcgc atgtggctac acgacgacat gccccgcaac tctgaatcac gggccatcag
     1621 ctacgcgctg aaagttatca gattactgta tccgtcagtg gagtgggttc agtcctttgc
     1681 tgacgaacgc tgcggactcg caggcgttgt gtatcaggcg tcgaattttg attttattgg
     1741 cagccatgaa agcacgttct acgagctgga tggtgaatgg tatcacgaga taacgatgaa
     1801 cgcgattaag cgcggagggc aacgaggcga gtatttgcga gctaataagg agcgtgccgt
     1861 ggtgcacaaa tttaatcagt atcgctacat cagattcctg aacaagcgag caaggaagcg
     1921 gctaaatacc aaactattca aggttcagcc ataccctaag tgatccccat gtaatgaata
     1981 aaaagcggta attaatactg ctgtttcatt tgaagcgcga aagctaaagt tttcgcattt
     2041 atcgtgaaac gctttcgcgt ttttcgtgcg ccgcttcacc cagccgttca taggcaattg
     2101 gcttaatgag ataatcaaat acaccacaac gtacggcttc agacaccgtt tccatatcac
     2161 tggctgcagt ggtaaacacc acgtcgccgg gataatgcgc ctgcaccagt tcatgcagta
     2221 aattaatccc tctaccgtca ggaagatagt tatcgagcaa gattagcccc ggcttaaaac
     2281 gctcgatcat cattcgggcc tgcgccaggt ttcccgccag taatatctga ctgaatccgg
     2341 gaatgtgacg aatatattcc gcatgcatct ctgccagcgg cgtttcgtcc tcaacgatca
     2401 atagggttaa tggagctgtc attaggtttc actttcggaa tatagattga aaataaggta
     2461 ccgcagggat cattatcttc gagagtgata acaccaccgc agcgcgttac gtagctggca
     2521 atcaagtaca acccaatgcc atgttcaccg ggctcgtcag cacgcgtact gacccctgct
     2581 caaatatttt gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg acttcaatca
     2641 ccacatcatc gccttcatcg ctgaggaata attcaacgct cttgtttcct tcatcgctac
     2701 gcaggctggc ttcgaaggcg ttatcaagta aattgccaac aatggcggca aactcggtgc
     2761 tatccagtcc tggcggcagt tgcgaaagct ggctaccggg gacgatgatc atttttagcc
     2821 ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc cgccacctgg cgatcggcaa
     2881 acgcctcgcg caggctgtca ataagctgtt gctgggcctg agactccccc tgcaccatcg
     2941 ccagcacgcg atcatactct ttcatctgca acagaccatt gagagtcgac atccaattga
     3001 gatgctcgtg tcgcaatgta cgaaggctct caacgtattg ttttatttgc gtcagttgcg
     3061 cattgagggt ggaaatttcg tctttactgc gaaagctgat aatggctccc agcaaatcat
     3121 cacctgaacg aatagcttcc cgattggcaa taacgctcag accgttaaag ttagccacca
     3181 catcctgacg tttttcatca atctgttcgg taaagaaatc ggcagacctg accacttcag
     3241 caatgggttt acccaaccat tgccgtccag gggaactcag ccccagcatc tttcttgcgt
     3301 tacgattgat ggcggtaatg taaccgtgcg gatccaccgc aatcagccct tcataaaccg
     3361 aactaaacag cgcctcttgc tgacggacca cccgtgcgat ttgctttggc tccatgccca
     3421 tcatctgccg acggatatgc gcggccagga accacgacag caacatcaga atccctaaca
     3481 gcacgacaaa cacacctgcc atcggtaata aaaactcagc ccgccagcta tcgattttac
     3541 tcaccaggta gccaatcgac accacgccga tgacttttcc atcgtcatca aagattggcg
     3601 ttttggcgcg catcgccata cccattgacc ctttcccggt aatgaagtag ctctcccctt
     3661 tctccagcgc gccctgtttg gtgaactgca taggataacc aattttctcc ggattaggat
     3721 ggtaaaggcg gatcgagtgc cggtcgccaa tcaccacata atcaaaatcg gtatctcttt
     3781 gtaatttgtt agcgatggtc gccagccgtt tgtagtcacg cgttttcacc gcagagatga
     3841 cactgtcatt ggaggcaata attttcgcct gattcattgc catgtcgcgt acatgaagcg
     3901 ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag ggctgcaatg acaaatattg
     3961 agaacaccag aatcagcaaa aagatacgca gcggaaatgt cagtctttgg aaaaatgcaa
     4021 actgtttatt ctcgttaagc tgcaacattg ttttccttgc gggttgtccc gagcctcttt
     4081 gacggatgta attaaaatgt tgaatcaaaa tacatagata ttaattatga tcgtggcaaa
     4141 ttttattcat taaatcaatt aaatcactta aacctgcgag tcatttcgta attacattaa
     4201 ttaaaaaact taaaaccatt aaacaaataa aaaaaccact aaccctatgt gaaataaatc
     4261 aaaatttcac gccgaaatac tccttaggat gtatagcgaa aggagaaaaa gatatacctc
     4321 gatcacaccc ctttctccca agtgaaaata aaaggttatc agtttgcaac attgaacaac
     4381 attcgttgca aatcgataac aacatgcacc ttcaggatac tatttattat gttcgtcaat
     4441 gatattttca cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc
     4501 ctgcatgaaa atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc
     4561 gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc
     4621 agtgaatccg ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc
     4681 gcctatgagc ggcacagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg
     4741 ttccgccagt gcggtttttc cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa
     4801 aacagcgcca cgcgactgaa acgctatgcc gaatcgctga aaaaattgcg tcatccaggg
     4861 aacaagattg gctgcattgt gatgaacgcc aatcccttta cgaatggtca ccgttatctg
     4921 attcaacaag ctgcggcaca gtgcgactgg tttcatctgt ttttagtcaa agaagattct
     4981 tcacgcttcc cctatgaaga ccggctggat ctggtgttaa aaggcaccgc cgatattcca
     5041 cgcctgactg tgcatcgcgg ctcagaatac atcatctccc gcgctacgtt cccttgctac
     5101 ttcattaaag aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc
     5161 cgtcagtacc tcgctcccgc gcttggtgta actcaccgct ttgtcggtac tgaacccttt
     5221 tgtcgcgtta ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc
     5281 tccgcagcgc ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata
     5341 tccgcttccc gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg
     5401 gtccctgcag tcacgctgca ttatttgcag aacctgcttg aacactcccg ccaggacgcg
     5461 gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg
     5521 ccgttgcagg cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc
     5581 aggatatcga cctgcaaatc aatagcagcg ttgagaaaca gtttggcgat gcaattcgca
     5641 ccaccattct ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg
     5701 acaaaggcgc actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca
     5761 gcggtatccc ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg
     5821 taaaactcgc acccgccgca gcatgttgtt tgtgcctggt gccaatgccg cgatggtcag
     5881 caactccttc atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgtggcatt
     5941 acgtgaaaaa gacaccgccc gccgcatggt ttatcacgcg ctgcaacatc cgctgtatcg
     6001 cgatattgaa accattgtgc gtgtcaacgc gctggattcc gaatggggtg ttaacgacct
     6061 ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca
     6121 ggatgttctg gatattgaaa aagagatcct gcgtatagaa aaagcctgtg gtcgtgaacc
     6181 cggcagcacc ggcctgctgg cggcgattga atctccgctg ggcattaccc gcgcagtgga
     6241 aatcgctcac gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg
     6301 caacctgcgt acagaacgct ccccggaagg aactgaactg ctgttcgcac gctgttccat
     6361 tttgcaggcc gcgcgctctg cgggtattca ggcgttcgat accgtctatt ccgacgctaa
     6421 caacgaagcc ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa
     6481 atcgctgatc aacccgcgtc agattgatct gctgcacaac ctctacgcac cgacccagaa
     6541 agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct
     6601 cggcgtggtt tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct
     6661 ggtgctctcc cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc
     6721 agaaaattga acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcactgaat
     6781 gcgatcttgc cgctttccag aactcaccaa agcaaaccta ccaggctgaa aaagcgcgcg
     6841 atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg ctcaggttta caggacggca
     6901 tgacggtttc cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg
     6961 acgtcatcgc gaagatgggc tttaaaaacc tgaccctggc gtccagctcc ctgagtgatt
     7021 gccatgcgcc gctggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctctg
     7081 gcctacgcgg tccactggcg gaagagatct cccgtggtct gctggcggaa ccggtgcaga
     7141 tccactctca cggcggtcgt gtgcatctgg tacagagcgg cgaactgaat atcgacgtgg
     7201 ctttcctcgg cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac accggtaaag
     7261 cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga
     7321 tgcttaccga agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg
     7381 ttgatttgat cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtacta
     7441 cccgtatgac cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg
     7501 tcaactctgg ctacttcaaa gaaggtttct ccatgcaaac cggcaccggc ggcgcttcgc
     7561 tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg
     7621 ccctcggcgg tattaccgcg acgatggttg atctgcacga aaaaggtctg atccgcaaac
     7681 tgctggatgt gcagagcttt gacagccatg ctgcgcaatc gctggcccgt aaccccaatc
     7741 acatcgaaat cagcgccaac cagtacgcta actggggttc gaaaggcgca tcggttgatc
     7801 gtctcgacgt ggtggtactg agcgcgctgg aaattgacag gcagttcaac gttaacgtgc
     7861 tgaccggttc tgacggcgta ctgcgtggcg cttccggtgg tcactgcgat accgcgattg
     7921 cctctgcgct ttccatcatc gtcgcgccac tggtacgcgg tcgtattccg actctggtgg
     7981 ataacgtgct gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg
     8041 gtatcgcagt taacccggca cgtccggaac tagcagaacg tctgcaggaa gcgggcatta
     8101 aagtggtttc cattgagtgg ctgcgcgaac gtgcgcgtct gctgaccggt gaaccacagc
     8161 cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg
     8221 atgttgtgca tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc
     8281 atgcggtgtc aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg
     8341 cctggctcaa gcaccatcct gttccactgg tctcctttac cgtggttgcg cctgggccga
     8401 ttaaagacag cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct
     8461 tagccacaaa gcagggctgg caaattcagg agcaggctgc actggtttcc gccagcgggc
     8521 cggagggcat gttgagcatt gccgccccgg ctcgcgatct caagctcgcc accattgagc
     8581 ttgaacatag tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg
     8641 aaattctctc ccgccgcgac tattcactgc cgcctcgccg ctgcctgttg tgcgagcaaa
     8701 gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg
     8761 aggcactgct gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg
     8821 tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaatctg
     8881 tcaccgaaac caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg
     8941 gaagatttcc accgcagcgc gctggcgatt cagggctggt taccccgttt cattgaattt
     9001 ggtgcctgta gtgcagaaat ggcaccggaa gcggtactcc acggattacg cccaattggt
     9061 atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggcagc
     9121 attttttctt tagggctgct atgtgcggca attggccgtt tgcttcagct caaccagccg
     9181 gttacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggcct gaccgatcgc
     9241 gaactgcgta ccaataattc acgactgacg gcaggtcaac ggttgtacca gcagcttggc
     9301 cttaccggcg cacgcggtga agccgaagcg ggttatccac tggtgatcaa ccacgccttg
     9361 ccgcattacc tcactctgct ggatcagggg ttagatcctg aactggcatt gctcgatacc
     9421 ttgctcctac tgatggctac caacggcgat accaacgttg catcgcgcgg tggcgagggg
     9481 ggcctgcgct ggctacagcg cgaggcgcaa acattattgc aaaaaggggg cattcgaacc
     9541 cccgccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt
     9601 ccaggcggca gtgccgacct gctgatcctt acctggtttt tagcacagat ttaattattt
     9661 aagcacttga taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag
     9721 ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt cttatccctg
     9781 tccccgacgg tatgccgccg caggcatggc attacttcgc tgtgtttgtg gcaatgattg
     9841 tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg gttactattt
     9901 gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta
     9961 atgcgcaaaa acaggcgctg aaatggggcc tggctggttt ttccagcacc acggtatggc
    10021 tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca
    10081 ttgccctttt cctggtgaaa ttcatggaca aacgcacgct gacgttgggt tatgcgattg
    10141 tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta
    10201 cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa atcattcccg aacgatccgt
    10261 ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt
    10321 catccatgtt tgtcaccggt gcggcaccaa acatgctggg tctggagttc gtcagcaaaa
    10381 ttgccggtat ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggggtta
    10441 tcttgcttat cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca
    10501 gtgaagaagt ggcaacctgg gcgggtgatg aactaaaaac catgggtgcg ctgacacgca
    10561 gagagtggac gctgattggc cttgtattgc tcagcttagg tttgtgggta tttggcagtg
    10621 aagtcattaa tgctactgcg gttggtctgc tggcagtttc gctaatgctg gctctgcacg
    10681 ttgtgccgtg gaaagacatt acccgctata acagcgcatg gaacacgctg gtcaacctgg
    10741 caactctggt tgtgatggct aacggcctga ctcgttctgg ttttattgac tggttcgccg
    10801 gtaccatgag tacgcacctg gaaggattct caccaaacgc aacggtgatt gtactggttc
    10861 tggtgttcta ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc
    10921 tgccggttat tctggccgtc ggtaaaggta ttccgggcgt accaatggaa caactgtgta
    10981 tcctgctggt gctgtctatc ggtatcatgg gctgtctgac gccgtatgca accggtcctg
    11041 gggtgattat ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa
    11101 tcttcggggt gatttacatc tctatgttgc tgttggttgg ctggccgatt ctcgccatgt
    11161 ggaactaata cgttcgccaa acgctgaata aatcatcacg cccaccaggt aagcccacct
    11221 ggcgggcttt tttatgattt aatagatagt attcagtgta tgtatgagtt ccacacccat
    11281 tatgaaagca ttctgccgta acgccgcgtt gctcgcggtt tctctgcttc ccttatcttc
    11341 tgccaacgcc gtagcgttgc aggcaaaaca gtatggcgat tttgatcgct atgtcctggc
    11401 cctctcctgg caaaccggat tttgccagag tcaacacgat cgaaatcgta acgaacgaga
    11461 tgaatgtcgc ctgcaaaccg aaacgaccaa caaagctgat tttctgaccg tacatggtct
    11521 gtggccagga ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg
    11581 cttcggttgc gctactcgcc caatcccgaa tctaccagaa gcacgcgcca gccgaatgtg
    11641 ttcatcgccg gaaaccgggt tatcactgga aacggccgct aaactaagtg aagtcatgcc
    11701 aggagctggc ggacgttcct gcctggaacg ctacgaatat gccaaacacg gtgcctgctt
    11761 tggttttgat ccggacgcat acttcggtac gatggtacgc ctgaatcaag aaattaaaga
    11821 gagcgaagcc ggaaaattcc ttgcggataa ttacggtaaa acagtgagcc gccgtgactt
    11881 tgacgccgcc tttgccaaaa gctggggaaa agagaacgtg aaagcagtta agctaacgtg
    11941 ccagggtaac cctgcgtatt tgactgaaat tcagatctcg atcaaagctg acgccatcaa
    12001 cgctccgctt tctgcaaact cattcttgcc acaacctcac ccaggtaact gtggcaaaac
    12061 ctttgtgatt gataaagcgg gttattaatc ctctgattta caaaacacgc taattcgtct
    12121 tccataacca tgccgattaa gaattataca taatgcgctg cattgaggaa ttccctgaat
    12181 gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat catcatgtga gttaaaccct
    12241 cgccgcctga cggtgagggt tttcttttgg gtatgtttat ctgcgacact cgcagtaccg
    12301 acgacatgga gtaaaaatgt ccagaccaac tatcatcatt aacgacctgg atgccgaacg
    12361 catcgatatt ctgctggagc aacccgccta tgctggtttg ccaatcgccg acgcgttaaa
    12421 cgcagagttg gatcgcgccc aaatgtgttc gccagaagag atgccacacg acgtggtgac
    12481 aatgaacagc cgggttaaat tccgcaatct tagagatggc gaagtgcgtg tgcgcacgct
    12541 ggtgtatccg gcaaaaatga ccgatagcaa tactcagctt tccgttatgg ctccggtagg
    12601 tgccgcactg ctggggctgc gcgttggcga ttccattcac tgggaacttc cgggcggcgt
    12661 tgcaacccac cttgaagtgc tggaactcga ataccagcca gaagctgctg gcgactacct
    12721 gctttaatcc catctgcaca agcgataact cgctttgccc agaggatgca ctcgcatcct
    12781 ctttacgcaa aatcagcaat ttttcctggt tctcactgtt cacttttgct gcaaaattat
    12841 tccgtttcgt ttgtgggaat aaccatggca gaagcatttt acatcctgat aggatttctg
    12901 attatggcgg cgattatcgt catggctgtc ctttacctcg aaaaccactc ctgacgatca
    12961 tattgttccc cccggcatcg cattcaccag acctgaaacc gccttctgcg ccgcctctgc
    13021 gctttgtgca ccgggtacca gaatcacctt acggcactcc tcttcacgtt tttcgaaaat
    13081 ctcatatccc cgggcggcct cttcaaacgg catatagtgg gtaacaattt cttctggttt
    13141 cagtaatcct ttctcaatta acggtaataa ttctcccagc catgcgtgaa cgtgggtctg
    13201 tcccatttta aacgtcaacc ctttatcaaa ggcgtcgcca aacaggaaac cgtgaataaa
    13261 tccagcgtag acgcccggta cgctaacaat gccgccacgc ctgaccgccg caatacactg
    13321 gcgcaacgct ttaccgctgc tgccctccag tttcaggtta gtcagcaccg tttccgtggt
    13381 gctgcctttc gcttcaaaac cgacggcgtc tattactgca tccacgcccc ggtgacctgc
    13441 cgtttgttca ataattgact gtgccggatc gctatcttca tcgaaattaa tcgggatcgc
    13501 gccgtagcgg tcagcggcga aacgcaagcg gtagggatga tgatcaacaa caaaaatctg
    13561 ttccgcaccg agcaaccgtg cacaggcgat tgtcaacaat cccacaggac cagcaccata
    13621 gactgcaacg cttgaacctt gttggatctg cacatttttt gctgcctgcc atgccgttgg
    13681 cagaatatca gaaaggaaaa gggctttatc atctgaaagc aaaggcggta ctttaaacgg
    13741 ccccacattc cctttaggga cgcggacata ttccgcctgc ccgccaggaa cgccaccata
    13801 caggtgacta taaccaaaca atgccgcggg agcaggtatc tgttttttat tgagcgcagc
    13861 gcctttaccc gcattggtat tttcgcaggc ggcatattgc tgtaatcgac agaaaaaaca
    13921 gtcgccacaa gcaatgacga acggaattac cacccggtcg cctttttgca aattttttac
    13981 gtccttcccg gtttcaacga cttccccata aattcatgac caaaaatatc gccatgttta
    14041 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttaccgta
    14101 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
    14161 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa
    14221 agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg
    14281 gagaattcct ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag
    14341 aaccagacaa atatcaggga taaatattat aatcatggcg aatgatttgt tcatgattaa
    14401 cagggagaaa gggttatgta taaggcaatc attatgccag ttgatgtatt tgaaatggaa
    14461 ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat
    14521 ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg
    14581 cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc
    14641 cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat
    14701 gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttatcgg ttctcgcaac
    14761 ccatcgattt cgacccatct gttaggttct aacgcctcga gcgtgatccg ccacgccaat
    14821 ctgcctgtac tggtcgttcg ctaacagata aaagtattta tgccgccctg cctggaacat
    14881 cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca
    14941 attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc
    15001 actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt
    15061 cgtacagtca cccgctgcga acacgccttt gacgttggtt tcacatttcg catcaatgat
    15121 aatctcgccc atgcggttac gttcgactgc gccttccagc cagttggtgt tcgggagcag
    15181 accaatctgg acaaaaatac cggccagttc gatgttgtga atattgccgc tgacacgatc
    15241 tcgatattcc agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt
    15301 cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt
    15361 catttctggc gcaaattcca gcagcgttac gtgctcaacg atacccgcca ggtcgattgc
    15421 cgcttccacg ccggagttac cgccgccgat aaccgctacg cgtttgcctt taaacagcgg
    15481 gccgtcgcag tgcgggcagt aggtcacgcc tttagtgcga tactgatctt cgcccggaac
    15541 gttcatgttg cgccattttg cacccgtcgc cacgataatg ctgcgtgctt tcagtaccgc
    15601 gccagaagct gtttcaatct ggtgtagacc accttcaacc gctgccggga tcagtttgct
    15661 ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc
    15721 tgccaacttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc
    15781 gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga
    15841 gtaaattgct gccgctgcac ccgccgggcc ggaaccaacg attaatacgt cataagcatc
    15901 acgcttgttc agctcttctg ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat
    15961 ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac
    16021 gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg
    16081 cttgatgcgc gggttcaata cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt
    16141 atggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg
    16201 ctccagcaga gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac
    16261 cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggtccct ggttggaacc
    16321 tgggttggtg atcaggaaag acggcttacg cactggcaag ctgttatctt ctttaaaggt
    16381 gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc
    16441 gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt
    16501 gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat
    16561 gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg
    16621 ggtgccgcag cacccgaaga attaggtgaa tttccgaagt atttcgcgtc agagcaaggc
    16681 gactagcctg tgaatccccg ggagcttaca caagtaagtg accggggtga gcgggcgacg
    16741 ccaacgccgc tatggcgtga aagacgaagg aaatttagat tttaccaacc aggtccagag
    16801 acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag
    16861 aagctacgta ctgagctgct ttgattttac gcagcagatc agacgcgtca cggccaatgc
    16921 cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac
    16981 ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg
    17041 ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt
    17101 tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt
    17161 cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg
    17221 ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt
    17281 cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct
    17341 atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat
    17401 gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga
    17461 actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa
    17521 tctgaactac cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt gcccttacaa
    17581 atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag
    17641 ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag
    17701 aacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca
    17761 gaggaacttc cttccccagt aaaagcgatt gaaaaacagg gcattacaat catcaaaacg
    17821 ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc
    17881 atctacctga ctcccgatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt
    17941 gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg
    18001 tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt
    18061 gtctacgtct tcgccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc
    18121 ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca
    18181 gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa
    18241 caatatgaag cctctggttg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag
    18301 caaatgaaag tgttaagtga caatgagaaa ctgatggacg atctgggggc aaatgtcacg
    18361 ccggctatct attacatgag taaggaaaat acgctgcaac aggccgtggg gttgcccgat
    18421 cagaaaacgc ttaatatcat tatgggaaat aaataaaagg taattcatgg atggttgaac
    18481 tatatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg
    18541 ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat
    18601 acatacgcaa aaaacattta ttaagtgaat ataccatgga agaaaaatat aaaccggagt
    18661 agtgtatggc caatctctac gacttgaaaa agttcgactt aaatcttctt gtcatatttg
    18721 agtgtattta ccagcattta agcattagca aagctgctga atcactatat atcaccccat
    18781 cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc
    18841 gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga
    18901 aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa
    18961 actttattat ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct
    19021 gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga
    19081 atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga
    19141 tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta
    19201 ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca
    19261 cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt
    19321 ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg
    19381 cagttacaga tttactggga atagtcccct atgaacttta taactaccac cgtgactttc
    19441 ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct
    19501 ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga
    19561 gtttttgata tataactaca gacagctact ataactttca tctatttatt cacagctcga
    19621 atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac
    19681 tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc
    19741 actcaatatt ctggaagctg cacgagagcg tattacctag acactcaaca ccctaccccg
    19801 cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga
    19861 acttgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aggcgc
//
LOCUS       DADLZM010000071        19906 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLZM010000071 DADLZM010000000
VERSION     DADLZM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.071"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            7..375
                     /locus_tag="JNY73_002386"
     CDS             7..375
                     /locus_tag="JNY73_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048377.1"
                     /translation="MFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSER
                     PIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFF
                     GILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="JNY73_002387"
     CDS             complement(411..1367)
                     /locus_tag="JNY73_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY8048378.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IITALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNY73_002388"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNY73_002388"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY8048379.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQCEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="JNY73_002389"
     CDS             complement(2605..3366)
                     /locus_tag="JNY73_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY8048380.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="JNY73_002390"
     CDS             3499..3909
                     /locus_tag="JNY73_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048381.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="JNY73_002391"
     CDS             complement(3871..4977)
                     /locus_tag="JNY73_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8048382.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="JNY73_002392"
     CDS             complement(4988..6121)
                     /locus_tag="JNY73_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8048383.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="JNY73_002393"
     CDS             complement(6114..7850)
                     /locus_tag="JNY73_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8048384.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNY73_002394"
     CDS             complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNY73_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY8048385.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNY73_002395"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNY73_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY8048386.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEETERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="JNY73_002396"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="JNY73_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNY73_002397"
                     /pseudo
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNY73_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="JNY73_002398"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="JNY73_002398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY8048387.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14883
                     /gene="ybiB"
                     /locus_tag="JNY73_002399"
     CDS             13915..14883
                     /gene="ybiB"
                     /locus_tag="JNY73_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY8048388.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPL
                     LAILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPV
                     GAFCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFS
                     DIGGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETT
                     AQWTERCLAGSEAIPESLKIQMACCLVATGEVATISDGLARVNQAF"
     gene            15024..16109
                     /locus_tag="JNY73_002400"
     CDS             15024..16109
                     /locus_tag="JNY73_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY8048389.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16338..16598)
                     /locus_tag="JNY73_002401"
     CDS             complement(16338..16598)
                     /locus_tag="JNY73_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8048390.1"
                     /translation="MKTINTVVAAMALSTLSFAVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16890..17129)
                     /locus_tag="JNY73_002402"
     CDS             complement(16890..17129)
                     /locus_tag="JNY73_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HAY8048391.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNR"
     gene            complement(17203..17916)
                     /gene="ybiX"
                     /locus_tag="JNY73_002403"
     CDS             complement(17203..17916)
                     /gene="ybiX"
                     /locus_tag="JNY73_002403"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY8048392.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            complement(17922..19906)
                     /locus_tag="JNY73_002404"
                     /pseudo
     CDS             complement(17922..19906)
                     /locus_tag="JNY73_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4618 a         5226 c         5378 g         4684 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggtgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gcactgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gtttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggctagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aaggggaagc agagatgctc
    14101 ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg
    14161 atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta
    14221 ttggcgattc tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat
    14281 ccaactcgcg tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac
    14341 ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg
    14401 ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt
    14461 gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc
    14521 agcgtttcgc atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg
    14581 tgggcgttac taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg
    14641 cagatcaatc tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct
    14701 ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt
    14761 tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg
    14821 gtggctacgg gtgaagtggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt
    14881 taatttttat ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa
    14941 attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca
    15001 cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc
    15061 acagttttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag
    15121 ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga
    15181 tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa
    15241 tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg
    15301 cggcacacga agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg
    15361 tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag
    15421 cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt
    15481 tccacggtcg cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag
    15541 ataatttccc gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg
    15601 tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc
    15661 cgacgaccaa tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg
    15721 aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg
    15781 gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga
    15841 ccactatcat catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag
    15901 cctttgccga gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg
    15961 gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg
    16021 gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc
    16081 aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa
    16141 caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg
    16201 gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta
    16261 ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc
    16321 cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg
    16381 gtggcggaag tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc
    16441 gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg
    16501 ttctgtgcct gggatgccgt taccggttcc gccgcgaaca cggcaaatga cagagttgaa
    16561 agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt
    16621 tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata
    16681 aaatcgaaga gaattgaccg ccttgttcaa ataaattgat tgatatctaa catacagata
    16741 aaccatttat ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt
    16801 taaaggcacg gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt
    16861 agttaacgta actggctgtc tttcgaacct cagcgattat atcctgtata cgctggtttt
    16921 tgtaaatctt tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga
    16981 cgggcttgcg ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga
    17041 atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg
    17101 gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt
    17161 taagtgtagc taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc
    17221 ccgcagcaga ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg
    17281 gcttttcagc gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc
    17341 gcggatcatc gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt
    17401 cacgcaatgc aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg
    17461 atgttgtcca aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct
    17521 taaaaacagc gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac
    17581 tgcgccatcc acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt
    17641 ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag
    17701 cacctcattt tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt
    17761 aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag
    17821 ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat
    17881 acaagttctc caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct
    17941 gtgagcaaga aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag
    18001 gcgacgtaat cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta
    18061 acccgatacc ccagtttggc atctgcgacc cagtaacctt ctgtaaatgc tggcgttccc
    18121 accgcgccgt ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag
    18181 atatcatcgg ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc
    18241 agcgatgagg aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg
    18301 gtatagccgc caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag
    18361 ccttcgacgc gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca
    18421 ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc
    18481 catttggtgc caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca
    18541 ctgttaccac tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa
    18601 acggcatagt taatatagac attgccgttt tccgtcaggt gatacagcgc cccggctttc
    18661 cagttcacca gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc
    18721 acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggtggt ggcactgtca
    18781 tattcagtat gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt
    18841 tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg
    18901 ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt
    18961 aacgtcaccg gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta
    19021 ctgacatcat gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg
    19081 agaattttat tactcacatc tttgatattc gccgtgcgtg actaggtcca gctattcaca
    19141 tcgctggtgg gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc
    19201 tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc
    19261 tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa
    19321 aagttatgag tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca
    19381 gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca
    19441 tgcagataat taagatacaa acgattcgct gtaccaaggc caaaagcgac agaaggggcg
    19501 acgccgtaac gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc
    19561 acattcaggc gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg
    19621 cggaaccagg cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc
    19681 ttgctgatca tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc
    19741 cctttaatca cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg
    19801 cgaataccat caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg
    19861 ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac ccacgc
//
LOCUS       DADLZM010000072        19779 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLZM010000072 DADLZM010000000
VERSION     DADLZM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.072"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..441
                     /gene="feaR"
                     /locus_tag="JNY73_002405"
     CDS             <1..441
                     /gene="feaR"
                     /locus_tag="JNY73_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HAY8048393.1"
                     /translation="QESMNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQ
                     KVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAI
                     RHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(613..942)
                     /locus_tag="JNY73_002406"
     CDS             complement(613..942)
                     /locus_tag="JNY73_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY8048394.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(947..1132)
                     /locus_tag="JNY73_002407"
     CDS             complement(947..1132)
                     /locus_tag="JNY73_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY8048395.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1129..3768)
                     /locus_tag="JNY73_002408"
     CDS             complement(1129..3768)
                     /locus_tag="JNY73_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY8048396.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLDVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3976..4965
                     /gene="ldhA"
                     /locus_tag="JNY73_002409"
     CDS             3976..4965
                     /gene="ldhA"
                     /locus_tag="JNY73_002409"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY8048397.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAVLELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5076..5412
                     /locus_tag="JNY73_002410"
                     /pseudo
     CDS             5076..5412
                     /locus_tag="JNY73_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
     gene            complement(5409..5675)
                     /locus_tag="JNY73_002411"
     CDS             complement(5409..5675)
                     /locus_tag="JNY73_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY8048398.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5949..9473
                     /gene="nifJ"
                     /locus_tag="JNY73_002412"
     CDS             5949..9473
                     /gene="nifJ"
                     /locus_tag="JNY73_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY8048399.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9841..10970
                     /gene="ompN"
                     /locus_tag="JNY73_002413"
                     /pseudo
     CDS             9841..10970
                     /gene="ompN"
                     /locus_tag="JNY73_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11111..11545
                     /gene="uspF"
                     /locus_tag="JNY73_002414"
     CDS             11111..11545
                     /gene="uspF"
                     /locus_tag="JNY73_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY8048400.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11721..12656
                     /gene="ttcA"
                     /locus_tag="JNY73_002415"
     CDS             11721..12656
                     /gene="ttcA"
                     /locus_tag="JNY73_002415"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY8048401.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12788..14155)
                     /gene="dbpA"
                     /locus_tag="JNY73_002416"
     CDS             complement(12788..14155)
                     /gene="dbpA"
                     /locus_tag="JNY73_002416"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY8048402.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14633..15616)
                     /gene="zntB"
                     /locus_tag="JNY73_002417"
     CDS             complement(14633..15616)
                     /gene="zntB"
                     /locus_tag="JNY73_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY8048403.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15871..17103
                     /gene="dgcM"
                     /locus_tag="JNY73_002418"
                     /pseudo
     CDS             15871..17103
                     /gene="dgcM"
                     /locus_tag="JNY73_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17124..17687)
                     /gene="smrA"
                     /locus_tag="JNY73_002419"
     CDS             complement(17124..17687)
                     /gene="smrA"
                     /locus_tag="JNY73_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY8048404.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17941..19395)
                     /locus_tag="JNY73_002420"
     CDS             complement(17941..19395)
                     /locus_tag="JNY73_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY8048405.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19392..>19779)
                     /locus_tag="JNY73_002421"
     CDS             complement(19392..>19779)
                     /locus_tag="JNY73_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8048406.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5198 a         5009 c         4667 g         4905 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt ttgtgcagat
      301 gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca ttggggattt
      361 tctgaccaga gtcatttctc aacggtattt aagcaacgct ttgggatgac gccaggcgag
      421 tatcgccgta aattccgcta actaattggc gttgcatgta ctgcaacgcc aattagttaa
      481 aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga gtttttgaat
      541 attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta tcccgatatt
      601 gcggttttgc tattaaaact ttcgcaccca tttaccgttt atcccttgta catattcccc
      661 cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat ctacagacac
      721 attattttgc tttgcccgtt gttggtaact cgctttacgt tcggcattaa tttcggttac
      781 taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa atgtttcacc
      841 tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc tgcttaccag
      901 cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag aaaagatcgc
      961 tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc tcatgctcaa
     1021 ttttaacgtt catattgata gtgatcggtt ccttaggtgc agcgacttca atgcgaggcg
     1081 tacagccaac cagcataaaa gatgacgtca acgcagccag taaaattttc attgtttttc
     1141 ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct gctccaacca
     1201 tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat tctcttcatg
     1261 atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac ctgtaatgtt
     1321 ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg accggctaat
     1381 ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt tcaccaccgc
     1441 atctgcggta tctttatcca gccgcaaggt cattggcccg ctatttgcca gccaaccatc
     1501 tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa atgccccgga
     1561 catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga gattattcag
     1621 ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagca cgttaccgcc
     1681 taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc acggataacg
     1741 cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt cggcaattcg
     1801 caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac ggaaaggcag
     1861 gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc ctttcaacac
     1921 gccgtgccct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa cctgagcata
     1981 cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct ggaatacagt
     2041 cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc agcgaccatt
     2101 tacccgaacc gggccaatct caccaacatg taaatcgcct ttaaagagga aataggtcgg
     2161 atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc cgccagtgaa
     2221 cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct gtgcgtcacg
     2281 tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca tcgtaccgta
     2341 ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt catgccactc
     2401 accttttcct gcgacatccc aggtggcatt catcggtgta aaactccctt ttccccagta
     2461 gcgccactgc cagcggccag cgtcagggag aaaatcattc gccagcccat ccatatgcag
     2521 cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac gtccgtcaac
     2581 accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga tgtccagcga
     2641 atctatgact cttccctttg aacgaagtaa cgcgcctggc tcaaaggtca gcgttgggtc
     2701 agacagactt ccacttagct gcgcaggtaa acgggcatat aaaatgaggt ccgcctgttt
     2761 cgcttcaccg gtcagctgca gaggcagttg actgttatcc atgcttaatt ttcctgggcc
     2821 aaaattaagc accgcgttgc ccttacccgc ttgcccctgg gtcagcacac tcagtcgtcc
     2881 gctgaccaga gcgttctcaa gccctgcctg ccagttgtca actttgacat ccagtcggcc
     2941 actcaaagga aaacctgcat acagccagct ccagcgacca tcgcttacgg tcaattgttg
     3001 acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg ccagcacgat
     3061 caattgcccg ctattttcct gccagtccag ctcggcatcc accagtgacg gttcctgcgg
     3121 caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca cgagcggcat
     3181 agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga catccagttc
     3241 gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt cgccctgata
     3301 acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc attcctgcca
     3361 gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg actgccactg
     3421 cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg ccagacaagc
     3481 agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg aaaggctggc
     3541 gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg gaatgattaa
     3601 acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga gcggtagcca
     3661 gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc agcagcgtca acggcacaag
     3721 aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg gtagttaata
     3781 tcctgatttc gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt taaccgttca
     3841 gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata tcgccatggc
     3901 tttcaattat atttgaaatt ttgtaaaata tttttagcag cttaaatgtg attcaacatc
     3961 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
     4021 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
     4081 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
     4141 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
     4201 tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag aactggggct gaaagtagtc
     4261 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
     4321 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
     4381 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
     4441 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
     4501 tatccaagtg cagcggtgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
     4561 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
     4621 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
     4681 gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
     4741 ggtatggacg tctatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
     4801 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
     4861 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
     4921 agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     4981 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     5041 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     5101 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaaa
     5161 tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt agcggtgaag
     5221 gcaggtgaac tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag
     5281 cttaatgaac tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg
     5341 accgcgaacc agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat
     5401 ttaatgaatt aatagctgcc acagctcccg gcggcaagtg actgttcgct acagcgtttg
     5461 ccgttgggta atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc
     5521 gaaccgccga tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaactt
     5581 ggcgctacgt gcgccgcagt cgcctgctga acaggctcac tactacacgc cgacaacaat
     5641 aaagcggcac accctaccca aaacgctgct cgcatctctt cttcctctga tcttcaagcc
     5701 aaacgacacc gccataaata ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc
     5761 gttgccccgc atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat
     5821 tcattactct gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa
     5881 ttgaaggatt actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag
     5941 ggtgtcatat gattactatt gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca
     6001 gtgaagttat cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg
     6061 cctgggccgg aaacggcttg aagaacgttt ggggagacac accacgcgtg gttgaaatgc
     6121 agtcggaagc gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa
     6181 catcgtttac gtcatcgcag ggtttgctgc tgatgatccc gacgctgtac aaactggcag
     6241 gcgaactgac gccatttgtc ctgcatgtag cggcacgtac cgttgccaca catgcacttt
     6301 ctatttttgg cgatcattcc gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt
     6361 gtgcggctaa cgtccaggaa gcgcaagact ttgctctcat ttcgcacatc gcaacgctga
     6421 agagccgcgt gccatttatt catttctttg atggttttcg cacgtcccac gaaatcaata
     6481 aaattgtccc gctggccgat gacactattc ttgatctcat gccgcaggcc gaaattgatg
     6541 ctcatcgcgc ccgggcgctc aacccggaac atccggtgat ccgcggtacg tccgccaatc
     6601 ctgacactta tttccagtct cgcgaagcca ccaacccctg gtacaacgcg gtctatgacc
     6661 atgttgaaca ggcgatgaat gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg
     6721 agtattacgg gcatccgcaa gcggaacggg tgattatcct gatgggctct gccattggca
     6781 cctgtgaaga agtggttgat gaattgctaa cccgtggcga aaaagttggc gtgctgaaag
     6841 ttcgtctgta ccgtcccttc tccgccaaac acttgctgca agctctgccg ggatccgtac
     6901 gcagcgtggc ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc
     6961 tggatgtgat gaccgcactg gcaggagcct ttaataatgg tgagcgcgaa accctgcccc
     7021 gtgtcattgg tgggcgctat ggtctttcat ccaaagaatt tggcccggac tgtgtactgg
     7081 cggtatttgc cgagctcaac gcggctaaac ctaaagcacg ctttacggtt ggtatttacg
     7141 atgatgtgac caatctgtca ctgccgttgc cggaaaacac tctgccaaac tcggcgaaac
     7201 ttgaagcctt gttttatggc cttggtagtg atggcagcgt ttccgcaacc aaaaacaata
     7261 tcaagattat cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca
     7321 aaaaagcggg cggcctgacg gtttctcacc ttcgcgtgag cgaacagccg attcgttccg
     7381 cttatctcat ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat
     7441 atcagatggc tgagcgttta aaacctagcg gcattttcct gcttaatacg ccgtacagcg
     7501 cagatgaagt gtggtcgcgc ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag
     7561 cgcgcttcta tgtgattaac gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta
     7621 ttaataccgt catgcagatg gcttttttcc atctgacgca aattctgcct ggcgatagcg
     7681 ccctcgcaga attgcagggt gcgattgcca aaagttacag tagcaaaggt caggatctgg
     7741 tggagcgcaa ctggcaggct ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc
     7801 aaccggtaaa tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgatt
     7861 tcgtgaaaac cgtaaccgcg gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg
     7921 cgctgccgcc agacggcacc tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg
     7981 ccgaagagat ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg
     8041 cttgcccaca ctcagctatt cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc
     8101 ctgccagcct gcattcgcta gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct
     8161 tgcaggtggc accggaagat tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga
     8221 aagaccgtca gaatccagag attaaagcca tcaatatgat gtctcgccag gaacatgtcg
     8281 aagaagagaa aatcaattac gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac
     8341 tggaacgtat tgatattcgt acatcgcagc tgattacacc gctgtttgaa tattcaggtg
     8401 cttgccccgg ttgtggcgag acgccgtata ttaaattgct gactcagctc tatggtgacc
     8461 ggatgttgat cgctaacgcc accggctgtt cttcaattta tggcggtaac ctgccctcta
     8521 caccgtatac caccgatgcc aacggtcgtg ggccggcatg ggcgaactct ctatttgaag
     8581 ataatgccga atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc
     8641 tgcgtctgct ggatcaattt gccgataaaa tcccggcgga attactgacg gcgttgaaat
     8701 cagacgctac gccagaggtt cgtcgagaac aggtcgcagc tttacgccag caactcaacg
     8761 atgttgccga agcacatgaa ctgctgcgtg acgcagatgc actggtggaa aaatcaatct
     8821 ggctgattgg tggcgacggc tgggcttacg atatcggctt tggcggtctg gatcatgtat
     8881 tgagtttgac ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaacaccg
     8941 gtggtcaggc gtcgaaagcg acaccgctgg gtgcagtaac taagttcggc gagcacggca
     9001 aacgtaaagc gcgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg
     9061 cgcagatttc tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag
     9121 cgtatccggg accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc
     9181 tggctctcag ccacgaccag atgcgccaac tcactgctac cggcttctgg ccgctatatc
     9241 gctttgatcc gcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc
     9301 cgtcagaagc actggaagaa acgttacttc acgagcagcg tttccgtcgt ctgaattcgc
     9361 agcagccaga agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct
     9421 atgacttcct agcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct
     9481 ggataaggat tatccaattc taaaaaaaaa agcccggacg actgttcggg cttgtctttt
     9541 tatatgttgg aaaatcagtg gcaatgcaat ggcccaacag aaaataaaat ctgtgacaat
     9601 aaaggcatat aacccgcgca gaataacgta tagaaaatta aaagtataat ttttattttg
     9661 tatgtattat cttcgataca ttcaccagac ttattatcat ttcagaggaa ttatttgatt
     9721 aaggttttac ttaaggcgta acaaatgata tttatcggca aactgaactt ctcctttagg
     9781 atgttttcac tccccgaaat ggggataaaa ggcaaataaa ataactaaag gatttattca
     9841 atgaaaagaa aagcactggc acttttaatc cctgccctgc tcgccgcagg tgctgcacat
     9901 gcagccgaag tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc
     9961 ctgcattatt tttctgataa ttcagcgaaa gatggcgacc agagctatgc gcgtctgggt
    10021 tttaaaggcg aaacccaaat taacgatcaa cttactggct acggtcaatg ggaatacaat
    10081 attcaggcaa acaacactga atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc
    10141 ggactgaaat ttgcagatta cggttctttc gattacggac gtaattatgg cgtaatgtac
    10201 gacatcgaag gctggaccga tatgctgcct aaatttggcg gtgactctta taccaatgca
    10261 gacaacttta tgactggtcg agccaatggc gtcgcgactt atcgtaatac tgatttcttc
    10321 ggtctggtaa atggtctgaa cttcgcggtg cagtatcaag gtaacaacga aggagccagt
    10381 aatggtcagg aaggcaccaa taacgaacgc gatgttcgtc atgaaaacgg tgacggctgg
    10441 ggtctttcca caacatatga tttaggcatg ggctttagcg ctggtgcggc atacacctct
    10501 tctgaccgca ccaatgacca ggttaaccat actgcggcgg gtggtgataa agcagacgcg
    10561 tggactgctg ggctaaaata cgatgctaac aatatttacc tagcaaccat gtattcagaa
    10621 acgcgtaata tgaccccgtt tggcgacagc gattatgcag ttgcgaacaa aacccagaac
    10681 tttgaagtta ctagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
    10741 ctaaaggccg tgaccttcac gctgctggtg gtgcagacaa cccggcaggt gttgatgata
    10801 aagatctggt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
    10861 cctatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg
    10921 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
    10981 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
    11041 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
    11101 aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttcagatt cagaattaac
    11161 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gatgatgcag aggttcattt
    11221 cctgacggta ataccttcac tgccctacta tgcctctctg ggtttagcgt attccgcaga
    11281 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
    11341 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
    11401 ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
    11461 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
    11521 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
    11581 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt
    11641 tatatcagat ttcttatgct gagcgttccg gcatgacgca tactcttctg atgccatata
    11701 acgaattgag tcgcttttaa atgtcgcaaa atcaagaaat tagtaagaaa gaacaataca
    11761 acctgaacaa attacaaaaa cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca
    11821 atatgattga agaaggcgat cgcatcatgg tttgcctctc cgggggtaaa gacagctata
    11881 ccatgctgga gattctgcgc aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg
    11941 ctgttaacct cgatcaaaag caaccgggct tcccggaaca cgttctgccc gagtatcttg
    12001 aaaagctggg cgttgagtac aagattgttg aagagaacac ttacggtatc gtgaaagaga
    12061 agattccaga gggcaaaacc acttgctcac tgtgttctcg gctacgtcgc ggtatccttt
    12121 atcgcaccgc aacggaactg gggacgacga agatcgcgtt gggtcaccat cgtgacgata
    12181 tcctgcaaac gttgttctta aatatgttct acggcggcaa gatgaaaggt atgcctccga
    12241 aactgatgag cgatgatggc aaacatatcg ttattcgtcc actggcctac tgccgcgaga
    12301 aagatattca gcgatttgcc gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg
    12361 gttcacagcc taacctgcaa cgtcaggtga ttgctgacat gttgcgtgac tgggataaac
    12421 gttatccagg gcgtatcgag acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc
    12481 tgtgcgatac caacctgttc gatttcaaag gcattaccca cggttctgaa gtggttaacg
    12541 ggggtgatct ggcgtttgat cgcgaagaga tcccactaca accggcgggc tggcagccag
    12601 aagaagatga aaatcagttg gatgagttac ggctgaatgt ggttgaagtg aaataaccag
    12661 gatagcgccc gatgcgcaag cgtatcgggc tactcttatg gaggccggat aagacgcggc
    12721 cagcagcgtc gcatccggca atcccgaata agatgtttac tcttgcaccc ggcaattcaa
    12781 catttcatta ttttaataac cgcacccggc acgtttttcc tttaatcttc ccgccctgta
    12841 actgtttcca tgctttatga gcaacagcct gacggaccgc gacatagaca tgcgccggat
    12901 gcacggcgat tttgccaata tctgcgccat caagcccgat atctcctgtc agtgcaccta
    12961 atacatcacc cgggcgcatt ttggcttttt tcccgccatc gatacacaac gttgccattt
    13021 ctgcttccag cgtcacaatg gaactattag ctggcggcgt ttgccagtta agttttatct
    13081 gcaacatgtc agaaatgata ttggcccgct gtgcttcttc cggagcacag aaactgatcg
    13141 ccagaccgct atttcctgca cgagctgtac gaccgatgcg atgtacatga acttcagggt
    13201 cccacgccag ctcaaagttc accaccagct caagcgattt aatatccaga ccacgcgcag
    13261 caacatcagt cgcaaccagt acacgggcgc taccgttagc aaaacgtacc agggtctgat
    13321 cgcgttgctc cagatcgccg tgtaacgaca atgcactttg ctctacttca ttcagcgcgt
    13381 cgcagacagc ctggcaatct tttttggtat tgcaaaacac cacacaagag gatggctgat
    13441 gcaagcttaa taaccgttgc aacagaggaa ttttgccttt actggatgtc tcataaaatt
    13501 gttgttcaat gggtggcaaa gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt
    13561 gcactcgtcc gctgattgcg gcgatggctt ccggccaggt tgccgaaaac agaagcgtct
    13621 gtcgagatgc aggcgcaaaa cggatgacat catcaatggc atcgctaaat cccatatcca
    13681 gcatgcggtc ggcctcatcc atcaccaaag tattcagcgc atccagtgat accgtgcctt
    13741 tttgcaagtg atccagcaaa cgacccggcg ttgccacgat aatatgcggc gcatgttgca
    13801 acgaatcacg ctgcataccg aacggttgac caccgcacaa cgtcaaaatt ttggtatttg
    13861 gcagaaaacg cgccagccga cgcaattcac ctgccacctg atccgccagt tcacgcgtag
    13921 gacacagcac taaagcctgg gtttgaaata gcgacgcatc aatttgctgt aacaagccga
    13981 ggccaaaagc cgccgttttg ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg
    14041 caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat ggttaaataa cccaactcat
    14101 taaggttcgt gagttgggcg ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct
    14161 attctcgtgg tcatcgacgc aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg
    14221 atgcgacaat ttaatcggtt cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg
    14281 gatcggatcg ggcactggaa caggatcgcc aggaatcggt tcagggacag gaatttgcaa
    14341 ataaataagt gtcgtcatat ttccctccgg tcattgggtg gactcttaaa gggtagacgc
    14401 tgataaataa caggcaaaaa aaagccgact catcaaagtc ggcgtcgtac gaatcaattg
    14461 tgctatgcag taattcaaaa aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg
    14521 acgttgcatt cacctgcaag ggagatattg ccctgaatgg gtagagagtt tattgacttc
    14581 gctcaaactt tgcggcgttt ttgtatacag acagccggaa aaattgcttt tgttacaacc
    14641 atttactacg gtgcaaccat aaagcaacac caccaataag aacaactaac agaatacaaa
    14701 aaattgaaaa tccgaattgc cacccgccgc cagggatccc accaaggttg acgccaaata
    14761 acccggtcag aaaggtactg ggtaaaaaga ccattgccat caacgacatt gtataggtac
    14821 gacgagctaa attttcctgc atcacctgag cgatttcatc cgccatcacg ccagtccgtg
    14881 ctatacaggc gtcgatttcg tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc
    14941 gacggcgttg gtcatcgctc atccacggca aacgttcact ggcaagacga gcataaacat
    15001 cacgttgcgg tgccatatag cgacgcatca caattaattg tttgcgcagc agagccagga
    15061 atccacgcgg tggaatttgc tgatcaagga gattatcttc aaggtcgata attttatcgt
    15121 gcagctgctc gataaattca ctggaatgat cggtcaacgc atcgcacaca tccaccagcc
    15181 atcccccgca atcggtcgga cccgtgccct cttccagatc gctcaccaca tcgtccagcg
    15241 ccagcacttt gcgttgtcgg gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg
    15301 cgaccagttg atcggggcgt tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg
    15361 tgccttcacc gaaacggctg actcggggcc gtgtgctctc gcccgccagc gcatcacgta
    15421 cgttattggg aagcagcggt gttgtcgcca gccattgggc gctatcatgg tgtacataat
    15481 taaggtggag ccagcaggga tgcgcttcat caatcacatc tgtattttcc agcggtttaa
    15541 cgccgcctct accatccagc atccaggcaa atactgcatc cgggacatta acgtccgatc
    15601 ccttaatcgc ttccacagtg cctccatcat caacgcatta ttttgtagtc tagccttctg
    15661 gccctgttac gcaacatctc atcaccccat taccctgaaa tgattaataa aattctgtct
    15721 aaattgaata caaaaagcaa aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag
    15781 cgtctatcat tatcagaatt atctgatcat atgacgtggc ttttttgcga tcggatagca
    15841 acaaaaattg ataaaaataa cgggatctca atgattacgc acaacttcaa taccctggac
    15901 ttactcacca gtcctgtctg gattgtttcg ccctttgagg aacagttaat ttatgccaat
    15961 agcgcggcgc gactgttgat gcaagacctc acgtttagtc agctacgaac cggaccctat
    16021 tccgtctcct cacaaaaaga actgccgaaa tacctctccg atctgcaaaa ccaacacgat
    16081 attatcgaaa tcctcactgt tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt
    16141 gttttgcgag agctgacaga aacagaaccg gtgattattt tcgaaggtat cgaagcgccg
    16201 gcaacgctgg gtttaaaagc cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt
    16261 tatgcgcgct tttttctgac taactctgca ccaatgttgt tgattgaccc gtcacgagat
    16321 ggacaaatcg tcgatgctaa cctcgccgcg ctcaatttct atggttataa ccatgaaacg
    16381 atgtgccaga aacataccta ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg
    16441 catgaaattt cgcatttacc cggcggtcat aaccctttga attttgttca taaactggcg
    16501 gatggttcta ctcgtcatgt gcagacctat gccggaccga ttgaaattta tggcgacaag
    16561 ctcatgttat gtattgtgca tgatattact gagcaaaaac ggctggagga gcagctggaa
    16621 catgctgctc accatgacgc gatgaccgga ttactgaatc ggcgacagtt ttatcacatt
    16681 acggaaccag gccaaatgca gcatctcgcc atcgctcagg attacagctt gttgctcatc
    16741 gacaccgatc gttttaaaca cattaacgat ctctatgggc attctaaagg tgatgaggtg
    16801 ttatgcgccc tcgcccgccc cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt
    16861 tggggaggcg aagagtttgt cttattgcta ccaagaaccc cactggatac cgcgctttcg
    16921 ctggctgaaa ctatccgcgt aagcgtggca aaagtgagta tttcgggctt accacgcttt
    16981 accgtcagca ttggtgtggc gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa
    17041 cgcgttgatg atgctttgta tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca
    17101 taagccgcgg atgcgtctcg agatcaacga ctgcgcttag cgtggcgctc ccagttttct
    17161 tgcttcgcct gcgccgtttt acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc
    17221 ggtagcgcgg tgcaatatgc ctgaacatca tcaaattcgg tcagccagcg cgccacatag
    17281 ctgcggacaa tattggcatg tgatttatct tcccgccctt taccatgaat aatcagcaca
    17341 ttacgcaaac catccgccag ggcttgttga atgaaactga acaccatttt gcggcactct
    17401 tccaccggct ggcgcaaaag attcaggctc gcctgttgcg gatatttacc actgcgcagc
    17461 ttatccagca ccccatgctg caacccttcc tgccgaaact ccagcggctg acttagcggg
    17521 atgatgtcga gaaatccggt agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc
    17581 ggcgcacgtt ggttacgcgt tggatgccag tggacatctg tagcacgttt cagcggctgg
    17641 acatcttcca tggcgtcaag aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg
    17701 caccttcaca cgtcgtgatt cagacgggtt aattgttcat acttactgca tcgttccctt
    17761 cacagattga aacaatactc tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt
    17821 atactagcct gttagcagta tcgaacagtt tagcccgagt ttaattcagc acaggctccg
    17881 atcaggatga tttattcata gcaggaacca acaaaaatgc gataaataac agaccactga
    17941 ttagcagaaa ggtaccgaaa atgccatagt ttgcgcctcc ctcagcatca attgactcaa
    18001 taatttggtt gaagccagta tttccgatgt ctggtcactt ctactgcgat taaaaaataa
    18061 agcacttctg gaaatgaagt cattcaccgt atggggaaca gaaaccattt tttcacccat
    18121 tctcgaaaaa tgaaaattag ttcgattatt gagaatagag ccacacaggg caataccaat
    18181 cgcgccccca agattacgtg ttaaattaaa tacaccagag gctaacttca gccgttcttt
    18241 tggaattccc cccagcgtta atgttacgat gggggccatc gcaaactgct gcaaaatccc
    18301 tctaattgcc tgtggaaaca gtaattcctg ccatccccat tcgtgggtta taggggtaaa
    18361 aagatacatt gaaaacacaa atccgcccag ccccgccatc aacagccatc gcagatttat
    18421 ttttttactt aaccagaagt aaaagggaac agaaaataac tggaaaatac ctgttgtgca
    18481 aacggcaaaa cctatctctt cggcatttaa tccgcgaatc tgccccaaaa acacaggtat
    18541 taaatataca gtagagaata tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt
    18601 gaaattttta tccttaaaag catgaagatc cattatcgga ttggatattg taagtgtacg
    18661 cgcagcaaat aaaataaaac tcaccaatgc cagaactgat gtcagcaaaa tagtattatc
    18721 atccagccat ccccagcgcg ctccttcttc aagggtatat tccagacatc ccagagtcat
    18781 ggccaacagg ataatactcg gataatcagc aactttcagt aaagagagat caggtttatc
    18841 aaaattaacc agaaaaggaa tactcaaaac taaatatatg ccaggtaata tattgatata
    18901 aaatagccat cgccagtcga ggttatcagt gagccagccc cccagagttg gccctagcgt
    18961 cggtgacagt gaggccagcg cactgactac cgcagcagcc aaccccaact ctttaccctg
    19021 gtaataaata aacgccgtcg tgaaaactaa agggatcatg gatgcgccag cagctccctg
    19081 taaagcacga aacaaaatca tgatctgaat attccatgca agtccacacg cgatactcat
    19141 taaagtaaat attcccgcgg atagagtaaa aagccaacga gtggaaaata cccgtgacag
    19201 ccacccagag agcggaataa caataatctc agcaattaaa tagctggtct gaagccaggc
    19261 catttcgtct ttacctgcgg acagaccacc acctatttca tttagggatg acgatacaat
    19321 ttgaatatca agctgagcaa taaataatcc catacacata ctggcgaaag caatatgttt
    19381 acgcatagac atcatctctc atcaacactg acggtgacgg acagtcccgg gcgaagaagt
    19441 tgctttaatt cctttgcgtc gtcaaatgca atccgtaccg ggacacgctg aacaattttg
    19501 gtgaaattgc cagtggcatt ttctatcgga atcaagctga aactggctcc tgtcgctggg
    19561 gaaaggcttt caatgtgacc gtgaaacact tcccctttga gaatatcagc acggatctca
    19621 gccttcattc ctggcttcat tcccgctatt tggttttctt tataatttgc atcaacccac
    19681 aactcactga ctggtactag tgaaactaac gatgtgccgc cttccaccca actgcctgta
    19741 tgggcgctgc ggtttgcgac aatgccatca atcggcgca
//
LOCUS       DADLZM010000073        19696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLZM010000073 DADLZM010000000
VERSION     DADLZM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.073"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            205..1302
                     /gene="mltA"
                     /locus_tag="JNY73_002422"
     CDS             205..1302
                     /gene="mltA"
                     /locus_tag="JNY73_002422"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY8048407.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1541..2347
                     /gene="tcdA"
                     /locus_tag="JNY73_002423"
     CDS             1541..2347
                     /gene="tcdA"
                     /locus_tag="JNY73_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY8048408.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="JNY73_002424"
     CDS             complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="JNY73_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY8048409.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELELRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="JNY73_002425"
     CDS             complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="JNY73_002425"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY8048410.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4238..4465
                     /locus_tag="JNY73_002426"
     CDS             4238..4465
                     /locus_tag="JNY73_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY8048411.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4816..5733
                     /locus_tag="JNY73_002427"
     CDS             4816..5733
                     /locus_tag="JNY73_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY8048412.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKIPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5752..6147
                     /locus_tag="JNY73_002428"
     CDS             5752..6147
                     /locus_tag="JNY73_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY8048413.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6140..7240
                     /gene="rlmM"
                     /locus_tag="JNY73_002429"
     CDS             6140..7240
                     /gene="rlmM"
                     /locus_tag="JNY73_002429"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY8048414.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7284..8015)
                     /gene="fucR"
                     /locus_tag="JNY73_002430"
     CDS             complement(7284..8015)
                     /gene="fucR"
                     /locus_tag="JNY73_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY8048415.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGAPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(8073..8495)
                     /gene="fucU"
                     /locus_tag="JNY73_002431"
     CDS             complement(8073..8495)
                     /gene="fucU"
                     /locus_tag="JNY73_002431"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY8048416.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8497..9916)
                     /gene="fucK"
                     /locus_tag="JNY73_002432"
                     /pseudo
     CDS             complement(8497..9916)
                     /gene="fucK"
                     /locus_tag="JNY73_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JNY73_002433"
     CDS             complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JNY73_002433"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY8048417.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JNY73_002434"
     CDS             complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JNY73_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY8048418.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13696..14343
                     /gene="fucA"
                     /locus_tag="JNY73_002435"
     CDS             13696..14343
                     /gene="fucA"
                     /locus_tag="JNY73_002435"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY8048419.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14371..15519
                     /gene="fucO"
                     /locus_tag="JNY73_002436"
     CDS             14371..15519
                     /gene="fucO"
                     /locus_tag="JNY73_002436"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY8048420.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JNY73_002437"
     CDS             complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JNY73_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY8048421.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTLAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JNY73_002438"
     CDS             complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JNY73_002438"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY8048422.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17863..19152)
                     /locus_tag="JNY73_002439"
     CDS             complement(17863..19152)
                     /locus_tag="JNY73_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY8048423.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLSMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4864 a         4823 c         5196 g         4813 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaagctt attttttgtg caaaatttgc
      121 gtcagggaga ggctgctcat ttcattaaac gcggcggttt gttatcttcg ttgcgcctta
      181 ttttttaacc tgaagaagag aacaatgaaa ggacgttggg taaagtacct tcttatgggc
      241 acggttgtgg caatgcttgc cgcctgctct tccaaaccaa ccgatcgcgg acagcaatat
      301 aaagacggga aatttaccca gcctttctct ctggtgaacc agccagatgc cgttggcgcg
      361 ccgattaacg ccggtgattt tgccgagcaa attaaccata tccgtaattc gtcaccgcgt
      421 ctgtatggca accagagtaa tgtttataac gcggtgcaag agtggctgcg cgcaggcggt
      481 gatacccgca atatgcgcca gttcggcatt gatgcctggc agatggaagg tgccgacaac
      541 tatggtaacg tgcagtttac aggctattac acgccggtaa ttcaggcgcg ccatacccgc
      601 cagggcgagt tccagtatcc tatttaccgt atgccgccaa aacgtggtcg tctgccgtct
      661 cgtgcggaga tctacgccgg ggcattgggc gataaataca tcctcgctta cagcaactct
      721 ctgatggata acttcattat ggatgtgcag ggtagtgggt atatcgactt tggtgatggc
      781 agtccgctta actttttcag ctatgcaggg aaaaacggtc atgcctatcg cagcattggt
      841 aaggtgctga tcgaccgtgg cgaagtgaaa aaagaagata tgtcgatgca ggcgattcgt
      901 cattggggca aaacacacag tgaagccgag gttcgcgagc tgctggaaca gaacccgtct
      961 ttcgtcttct ttaaaccgca atcttttgca ccggtgaaag gggcaagtgc ggtgccgctg
     1021 gttggccgtg cttctgttgc ctccgatcgt tcaattattc cgccaggtac taccttgctg
     1081 gcagaagtgc cgctgctgga taataacggc aaatttaacg gtcagtacga attgcgtctg
     1141 atggtggcgt tggatgtcgg gggcgcaatc aaaggccaac acttcgatat ctatcaaggg
     1201 atcgggccgg aagccggaca ccgcgcaggt tggtacaacc actatggacg ggtttgggtg
     1261 ctgaaaacgg ctccgggcgc aggtaacgtc tttagcggct gatgtgatat tctgtatgcc
     1321 gtttttacgg atatgacagg gtgaggggta acctcacatc ctgattattg actaagtgcg
     1381 cgttgttcat accggatgcg gcgtgaacgc cttatccggc ctacaaatca tgcaaatcca
     1441 aaatattgca ggagttatgt aggcctgata agcgtagcgc atcaggcatt tatgcgttta
     1501 gggtgaggga taacctcgcc ctttttaatt ctgaggtgct atgtctgtgg taattagtga
     1561 tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact
     1621 gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga
     1681 agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt
     1741 caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga
     1801 agtcatggcg gagcgcattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga
     1861 tttcgtgacg ccggataacg tagcgcaata tatgagtgcc ggttattcgt acgtgattga
     1921 tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat
     1981 cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac
     2041 cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag
     2101 cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtggattgcg tgttttctac
     2161 tgaagcgctg gtgtacccgc agtcagacgg tacggtgtgt gcgatgaaag ccacggcaga
     2221 agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac
     2281 ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca
     2341 gggttaaaca atcaggtcgg atgagacgcg aacgtcttat ccgacccggt tctgtgctta
     2401 aacctgcttc gtcgcagcga taatcgcctc gcttaacgca tttaacccct ggctgcgtga
     2461 ggcgctaagc tgcgcacgta attccagctc atcaaacaat gccagcggcg actgtgcctg
     2521 taattcggcg gcggttttcc cctcaacggc agtcaacaac accgccaaca ggccgcgcac
     2581 aatgcgccct tcgctgtcgc caaagaagtg cattttgccg ttttcagcca cggtatatcc
     2641 cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg
     2701 caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcga
     2761 caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc
     2821 ggcgaattgc gggtttgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc
     2881 accagcgcat ccacatcact ctttgtatta tatggcgcaa atgaggcgcg cagtgtgccg
     2941 gttacgccta attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata
     3001 ccgtactccg ccagtagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc
     3061 agcaggctgg aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc cagcgcttct
     3121 tctgcaagcg ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgcc
     3181 agccattcca gcgccgcgct taatcctatg acgccagcga catttggcgt tcctgcttcc
     3241 agtttccacg gcgcagattg agtggtgaaa ccgtcaaaac tcacttcgtg aatcattttg
     3301 ccgccaccca gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg
     3361 atacccgtcg ggccatacag tttgtgacca gagaaagcat agaaatcgat atccagttgc
     3421 tgaacatccg cggggaaatg caccgccccc tgagcaccat caaccatcac caccatcccg
     3481 gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt cacgttcgac
     3541 atctgaccca acgccagaat ccgactacgg ggagtaatca gttctggcaa caaatcgaca
     3601 tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctctagt ttgttgggcg
     3661 accatcagcc aggggacgag gttagcgtgg tgttctgcca cgctgacaat aatctcatcg
     3721 cccggttgca gacgcggacg cgcatagcat tgtgccacca tgttgatgga ttcagtggtg
     3781 ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct
     3841 cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg
     3901 tttccggcgc tcaggctgta aaactgtcga gtggcttcaa ccacggcttc aggtttaagc
     3961 gcggtcgcgg cgctgtcgag atagacgccc gcatcctgta gtgcgggaaa ctgggcgcga
     4021 aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc
     4081 aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga
     4141 agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat
     4201 actattaaga tttattaatc gctttaagga gaagatgatg aaaaagactg ccgcaattat
     4261 ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca
     4321 caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata acgataccgg
     4381 tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa
     4441 agagatggtc gaactggaac agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt
     4501 aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag
     4561 gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca
     4621 caaccgtaag ccaaaagttc accagaatac gcattccgat aaaacttttc gttccggctc
     4681 aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc
     4741 tcagattaaa aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat
     4801 caaagtttaa aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat
     4861 gccgcagcac gccatttaag ttttactcgc gcagcagaag agctttttgt gacccaggcc
     4921 gcagtaagtc atcaaatcaa gtctcttgag gattttttgg ggctaaaact gttccgccgc
     4981 cgtaatcgtt cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag
     5041 atattttcgc aattaaccga agcgacgcgt aaactccagg cccgtagcgc caagggggcg
     5101 ttgacggtca gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc
     5161 tttaattcag cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat
     5221 aagctggcgg atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta
     5281 cgggtggaaa aactgtacgc cgaatattta ttgccggtgt gttcgccgct actgctgaca
     5341 ggcgaaaaac ccttgaagat cccggaagat ctggctaaac atacgttatt acatgatgcg
     5401 tcacgccgtg actggcagac atatacccga cagttggggt taaatcatat caacgttcag
     5461 caagggccaa tttttagtca tagcgccatg gtgctgcaag cggctattca cgggcaggga
     5521 gtggcgctgg caaataacgt gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc
     5581 ccgtttaatg atgttctggt cagtaaaaat gctttttatc tggtttgtca tgacagccag
     5641 gcagaactgg gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa
     5701 caagaaaaat tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc
     5761 cgttttatgc tgattttcgc cgccattagc ggcttcattt ttgtggcact gggcgctttt
     5821 ggtgcgcatg tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccaaaccggt
     5881 ctcgaatacc aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt
     5941 cgcatcagta tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc
     6001 agcggcagcc tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt tgtcacaccg
     6061 gttggcggcg tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta
     6121 aagcgcaagg gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa
     6181 agagtgcgcc gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg
     6241 cgtgaaagag aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa
     6301 gttaatccgt gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg
     6361 ggaactcctg cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttaca
     6421 gggtgtagta gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa
     6481 agagttgctg aaattctgcc gtaagttcac tgttccgcta cgcgctgcct tgcgcgatgc
     6541 tggggtgctg gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc
     6601 accaggatgc tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg
     6661 cattccgcgc ctgaaatttc cggcagaggc gccgagtcgt tccacgctca aactggaaga
     6721 ggcatttcat gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg
     6781 ggcggtggat ttaggcgctt gccctggcgg ctggacctac caactggtga agcgcaacat
     6841 gtgggtttat tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccgggcaggt
     6901 gacgtggctg cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata tctcctggat
     6961 ggtatgcgat atggttgaaa aaccggctaa agttgcggca ttgatggcgc agtggctggt
     7021 taatggctgg tgccgtgaaa ccattttcaa tctcaaactg ccgatgaaaa aacgctacga
     7081 agaagtatca cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc
     7141 tcagattcag gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg
     7201 catctgggct gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg
     7261 tctccggcct gctacccttt ccgtcaggct gttaccaaag aagttgcaac ctggcgctca
     7321 tcagaaataa tgtgcgttac ctcatccaga tacccgatgc gggcttcccc tgaacgatta
     7381 aatttacttt tatcaatcaa taacaacgat tgcgcggcac gttttaatag catcgatttg
     7441 taatcagcgt tgatcgcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa
     7501 gaaaaaataa acagatcgat ttccagcgat tttagttggg aaatcagcga gggattgacg
     7561 taacagccat atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt
     7621 ttaccgagtt catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc
     7681 aactggcgtg ccagatacca gcaagttgaa ctggcgtcta aggctatcac catcccttct
     7741 tcaatccacg cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc
     7801 ctgatgtgaa agggagcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca
     7861 tgattgcgca gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct
     7921 ttactgacct ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc
     7981 aggtcgacta tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac
     8041 cgcccgcgtc atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga
     8101 atattcccgt acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt
     8161 tcataaaacg caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc
     8221 gcattacggt aacgtcgttc tacttcagga tcgagagtgt caccctctac cgccgccatc
     8281 atcaccagcg gcggggcata actgtccagc tcaaataacg ggataatcgc ctggagcaag
     8341 tcgctcacca acaggccatc agcgcggatc acctgcggtc ccatcgaatg ggcgggaaag
     8401 tgagcatcgg aaaaaataat ttcatctcca tgtcccatct ctgccagcac tttcaatagt
     8461 tcgggagaaa ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg
     8521 ttcagtttgc gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg
     8581 gctgttaaat tcccctacgc cataccaacc gaacagcgca gctcctgcga cggtcgtttc
     8641 ggcatcgtcg agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa
     8701 tgtgttgcga cttcctccac cgactaacaa taattcatag gctttaaagt gcccgatttt
     8761 ttccagcatc tgtagattgc gctgtaattg cgcagttaac ccttccagcg ccgcgcgata
     8821 gaaatgcccc cgcgtggtat taagcgtcac tccttgccag ccagcgttct gacacgacaa
     8881 taaatcacac tgcattttca caccatccgc gccaggtgcg atcagacgag cttcttcaat
     8941 caacatttgc cagggtgttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc
     9001 ggatgccagc cattgcatac ctgggttata caaccatgcc tggctatcta gttcgcaggt
     9061 ggaaccggcg tactgactta acagcgaagt atcaacctag gcgctgcgaa ccattaaaat
     9121 ttcccatgta ccggaggaga gcacgggttc attttgttcg gcaccagcgc caaaaagggc
     9181 gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc
     9241 tgcggcgctg ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag
     9301 tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc
     9361 cagcatctgg ctggttccgg ctatcgtgat atcagtagtg aattcgccgg ttaaacggtg
     9421 gttaatcagc gacgaaatga agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt
     9481 ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga cgccagaaat
     9541 agcctgcaac cgctgtgcgg agattaaccg ttcaatattg tccataaccg ctgctgttcg
     9601 cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc
     9661 atccacacca aaggtggtga cggcgatacc gcggatgtgg cattcagtca gttcactatt
     9721 gatttgccga cagcaatcag caaagcgttg caaaatggca gtctaaagac cactggtgcc
     9781 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
     9841 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
     9901 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta
     9961 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggtgtaa
    10021 tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa
    10081 tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    10141 gcatacatac cgggatacgc agcatggagg caagggtgat aaagtctgcg ccaacgtggc
    10201 cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa
    10261 acggcccttt accagtgagg cgcggtgcaa accaggtggt tggccaggtt gagttggtgc
    10321 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    10381 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    10441 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    10501 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    10561 tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac
    10621 ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta
    10681 cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc
    10741 cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca
    10801 cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg
    10861 tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg
    10921 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    10981 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    11041 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    11101 cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    11161 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    11221 gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    11281 ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    11341 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    11401 ggatgccttt ctggctgtga gctgccagag ccgctgccag gtaaacagcg ccggggcgtt
    11461 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac
    11521 tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt
    11581 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    11641 cgacggcagc tccgcaggta tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg
    11701 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    11761 ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt
    11821 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    11881 cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac
    11941 aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    12001 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    12061 aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa
    12121 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg
    12181 tgcgaagcga ctgatgagcc aggtaccggt gatacgacca ataaagaagc acaccatggt
    12241 tccggttaaa tagttagcgg caaagcctgc agtcatacct ggaatttctt ctacagcgta
    12301 gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac
    12361 cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg
    12421 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    12481 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccaa
    12541 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    12601 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    12661 cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac
    12721 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    12781 gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcgcc
    12841 caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    12901 tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    12961 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag
    13021 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    13081 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    13141 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    13201 aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa
    13261 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    13321 gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgcccgttt
    13381 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    13441 cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc
    13501 ggtcattttt aacggggtta taaaaagaga acttgcagtt atttactgtg attagttgag
    13561 ctaggtcaca aaatataagc ttgtgaattt ctttcttgtt aatcaggaag aagatggtgg
    13621 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    13681 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    13741 gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag
    13801 gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt
    13861 gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt
    13921 ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    13981 gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt
    14041 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    14101 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    14161 gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa
    14221 gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    14281 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag
    14341 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    14401 gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat
    14461 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    14521 accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac
    14581 ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac
    14641 ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc
    14701 aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    14761 agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac
    14821 tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    14881 ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc
    14941 gcgacgggtg tcgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    15001 gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    15061 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    15121 atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    15181 ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat
    15241 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    15301 gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac
    15361 cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg
    15421 gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    15481 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    15541 ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    15601 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    15661 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    15721 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    15781 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    15841 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    15901 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    15961 tcgttgcctg atgcccggcc agtgtcactt tgaccgccag tgtggcggct aagtcatcgg
    16021 cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
    16081 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta
    16141 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    16201 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    16261 aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa
    16321 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    16381 ctatggctgg ttgagaaaac aggaagaggc ttcgcaaaac gaggcctctg ggagagatta
    16441 atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt
    16501 catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc
    16561 gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt
    16621 acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt
    16681 gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc
    16741 tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc
    16801 ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag
    16861 gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac
    16921 agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc
    16981 gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc
    17041 agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac
    17101 gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat
    17161 gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagctc
    17221 gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc
    17281 ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg
    17341 ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt
    17401 atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg
    17461 gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag
    17521 gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc
    17581 cggcaggtta cccgccaggc ccataataat ggagatatct gtgtggtggc ctttaccagt
    17641 cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg
    17701 ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact
    17761 ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg
    17821 ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta
    17881 gaagattgcg gagattgcaa tcagccccat cacgacaacg aatacgttgc tgatgtgacc
    17941 gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat
    18001 catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt
    18061 ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat
    18121 ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg
    18181 tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc
    18241 catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt
    18301 ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa
    18361 gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg
    18421 ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga
    18481 gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt
    18541 tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg
    18601 gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat
    18661 cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat
    18721 cagcgacaga atcgcacgcg gcggtggcgt catactcagc tggtgagaca tgaagctttc
    18781 aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta
    18841 gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat
    18901 gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa
    18961 cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc
    19021 gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca
    19081 catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg
    19141 agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag
    19201 cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt
    19261 accgctgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct
    19321 tgatcccaaa tacaataatc tgcttggaag atagattatc ccaacgcttt actctgtaaa
    19381 tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc
    19441 cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc
    19501 gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgcgtt
    19561 gcaacataaa ttgtatggca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga
    19621 aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg
    19681 gcaacaaacg tcaccg
//
LOCUS       DADLZM010000074        19418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLZM010000074 DADLZM010000000
VERSION     DADLZM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.074"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..352
                     /locus_tag="JNY73_002440"
     CDS             <1..352
                     /locus_tag="JNY73_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8048424.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            509..1258
                     /locus_tag="JNY73_002441"
     CDS             509..1258
                     /locus_tag="JNY73_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048425.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1280..1846
                     /gene="yieF"
                     /locus_tag="JNY73_002442"
     CDS             1280..1846
                     /gene="yieF"
                     /locus_tag="JNY73_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY8048426.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1900..3237)
                     /locus_tag="JNY73_002443"
     CDS             complement(1900..3237)
                     /locus_tag="JNY73_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8048427.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLANEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3401..4066
                     /gene="yieH"
                     /locus_tag="JNY73_002444"
     CDS             3401..4066
                     /gene="yieH"
                     /locus_tag="JNY73_002444"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY8048428.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4133..4606
                     /locus_tag="JNY73_002445"
     CDS             4133..4606
                     /locus_tag="JNY73_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY8048429.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4655..5242
                     /locus_tag="JNY73_002446"
     CDS             4655..5242
                     /locus_tag="JNY73_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048430.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGMKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5304..6026)
                     /locus_tag="JNY73_002447"
     CDS             complement(5304..6026)
                     /locus_tag="JNY73_002447"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY8048431.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6028..7203)
                     /locus_tag="JNY73_002448"
     CDS             complement(6028..7203)
                     /locus_tag="JNY73_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY8048432.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7230..8846)
                     /locus_tag="JNY73_002449"
     CDS             complement(7230..8846)
                     /locus_tag="JNY73_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY8048433.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVALENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8932..10329)
                     /locus_tag="JNY73_002450"
     CDS             complement(8932..10329)
                     /locus_tag="JNY73_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY8048434.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10345..12222)
                     /gene="bglF"
                     /locus_tag="JNY73_002451"
                     /pseudo
     CDS             complement(10345..12222)
                     /gene="bglF"
                     /locus_tag="JNY73_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
     gene            complement(12356..13192)
                     /gene="bglG"
                     /locus_tag="JNY73_002452"
     CDS             complement(12356..13192)
                     /gene="bglG"
                     /locus_tag="JNY73_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY8048435.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMCLAINIERVRK
                     EH"
     gene            complement(13478..14203)
                     /gene="phoU"
                     /locus_tag="JNY73_002453"
     CDS             complement(13478..14203)
                     /gene="phoU"
                     /locus_tag="JNY73_002453"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY8048436.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
     gene            complement(14218..14991)
                     /gene="pstB"
                     /locus_tag="JNY73_002454"
     CDS             complement(14218..14991)
                     /gene="pstB"
                     /locus_tag="JNY73_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY8048437.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(15083..15973)
                     /gene="pstA"
                     /locus_tag="JNY73_002455"
     CDS             complement(15083..15973)
                     /gene="pstA"
                     /locus_tag="JNY73_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY8048438.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(15973..16932)
                     /gene="pstC"
                     /locus_tag="JNY73_002456"
     CDS             complement(15973..16932)
                     /gene="pstC"
                     /locus_tag="JNY73_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY8048439.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(17019..18059)
                     /gene="pstS"
                     /locus_tag="JNY73_002457"
     CDS             complement(17019..18059)
                     /gene="pstS"
                     /locus_tag="JNY73_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY8048440.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(18307..19380)
                     /locus_tag="JNY73_002458"
     CDS             complement(18307..19380)
                     /locus_tag="JNY73_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8048441.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
BASE COUNT         5061 a         4926 c         4507 g         4924 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcagg gcgctggaca atgtgttaca
       61 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
      121 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
      181 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
      241 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
      301 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
      361 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
      421 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
      481 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
      541 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
      601 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
      661 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
      721 tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
      781 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
      841 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
      901 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt
      961 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
     1021 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     1081 cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt
     1141 agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
     1201 cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata
     1261 gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     1321 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     1381 catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     1441 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
     1501 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     1561 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
     1621 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     1681 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
     1741 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
     1801 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     1861 agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac
     1921 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     1981 cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
     2041 cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
     2101 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc
     2161 agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa
     2221 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
     2281 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
     2341 actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat tcgccagacc
     2401 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa
     2461 agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
     2521 aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat
     2581 ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga
     2641 ggccagaata gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag
     2701 attaccgatg ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttcagccc
     2761 catcatgccg ataaacaggc cgataccgct ggtaataccc acacgcagac ttaccggaat
     2821 gttagcaatc atccagtaac gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc
     2881 ccagaagatt gcgcccatac cgatctgcca cggcagcccc attgcctgga caaccacaaa
     2941 agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa
     3001 tcccatcata atgctaccga aagcagcaat cagacaggtt gttacgaaga cggcactggt
     3061 gtccatgcca gcaacgccca gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag
     3121 gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt
     3181 aaaaacgcgt tccagcatcc tctgaccaga agtctgggtg gtatgttgat gactcattat
     3241 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatgc gacaataggc
     3301 gcgtttgtga gagacttttt attggattta cttatacggc aacgattgcg ttgcgcaaat
     3361 cggctctacg aaaacgttaa actgattaaa aaggaaaggc atgtcccgga tagaagcggt
     3421 atttttcgac tgcgacggta cgctggtcga cagtgaagtc atttgctccc gcgcatatgt
     3481 aacgatgttt caggaatttg gtattacgct cgatcctgaa gaggtattca aacgtttcaa
     3541 aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt gaacatggtg ttacgttagc
     3601 gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct cggctgttcg attcagaact
     3661 ggaagccatc gaaggggctg gagcgctcct gtcagcgatc actgcgccaa tgtgtgtggt
     3721 atctaacggc ccaaataaca aaatgcagca ttctatgggc aagctgaata tgttgcacta
     3781 cttcccggat aaactgttca gcggctacga tattcagcgc tggaagccag acccggcgtt
     3841 aatgttccat gcggcaaaag cgatgaatgt aaatgtagaa aactgcattc tggttgatga
     3901 ctcagttgcc ggtgcgcaat ctggtatcga cgcaggtatg gaagtgttct acttctgcgc
     3961 cgacccgcac aataagccga tcgttcaccc gaaagtcacc acctttaccc atctttcgca
     4021 gttaccggaa ctgtggaaag cgcgtggttg ggatattacg gcatagttct tcacactccc
     4081 ttcacttacc ccgcttaaat tggcgctcaa aggtaagtaa agggagtttg atatgtctgt
     4141 ttcacgtcgg gtaatacatc acggacttta ttttgcggtt ttaggaccgt taattggtgt
     4201 tctgtttctt gtcctctaca tattcttcgc aaaagaaccg ctggttcttt tggtaataat
     4261 acaggtactt ccattgtttc tcttattgtc gattactacg ggagctattc ctgcattgtt
     4321 aaccggcgtg atggtcgcct gtctgccgga aaagatcggg tcacagaaaa attatcgttg
     4381 tctggctggt ggcataggtg gcgtcgttat caccgagatc tattgtgcag ttattgtaca
     4441 tattaagggc atggcttcct cggagttgtt tgaaaacatt ctttctggtg acagtctcgt
     4501 tgtccgcatc attcctgcat tgctggcagg tgtggtgatg agcagaatca ttacccgtct
     4561 gcccggattg gatatttcat gtcctgaaac agactcttta agttaagcgg gatactttat
     4621 ctttgggcta ctcaaaagca gacaggatgt tactatgact caaaatatca ggccgttacc
     4681 ccaattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg ataaaaccgt
     4741 agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct tttattgcgt
     4801 tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg ctgaaaagtt
     4861 tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt atgatgaaga
     4921 ggacgaattt gccggtatga agaacacata tcctgatgaa atgctgaaag agttggttga
     4981 acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt gtggcgattt
     5041 ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg atgaatttgc
     5101 caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag ctaaatgcct
     5161 gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg gcaagctgag
     5221 actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag cgtaattcat
     5281 acatccggcc ttatttcttc agcttaaccg aacgctaatt ctgcggcagc ggctttatca
     5341 gcgattacca tcagcgaagg atgcagctgc aacacggaag caggaacatc ttcggtaacc
     5401 ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac aattatcagc
     5461 aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc aggcactaac
     5521 gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc ctggatcggg
     5581 aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca aaaatgacca
     5641 tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag tttctgatca
     5701 tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat ccccgcaggg
     5761 gtgaaaaaca gattacgcag attggtaatc gttacgcctt ctccctcttt gccgcgaaag
     5821 ggaatttcat cgaaattata gaaatagcag ttatcgtacc aaggcttacc tttaaccagg
     5881 gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc caggttaaca
     5941 cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct catttcctgg
     6001 taatcttcgg taatgattaa tttcatctta atgccttatt ttaaatttct gtacgaaggc
     6061 tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt cctggtaatc
     6121 aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct tgccggttac
     6181 gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa tagctgccgg
     6241 atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa caccactgaa
     6301 tgtggccagc cagccaaagc tttccagatg attcattccg gaaaccagcg cctggtaccc
     6361 gccttgtgaa agccctgcca gcgcgcgacc atcggcatct ttacggacac taaaacgctt
     6421 gctaatcagc gggataatat cgttcatcag ttcgcgatct gccgctttag cattcagcgg
     6481 ataaaagact ttacgtcttt cctgaggcac gaaatcttcg ggaataatgc ccttcgcatc
     6541 ggtttcggta tccgggatca ccaccagcat cggtttaatt ttcccttcag ccagcaggtt
     6601 atccatgatt tgcgggatac gcccctgatc gatagcggaa cgtccagtat caccaaaacc
     6661 gtgatagaaa tagagcactg gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca
     6721 gacatacatc tgacgttcag attgcaatgc gttggagtgg taagttatgg cgatcaaatc
     6781 accatgcgca acagaacgcg tatccagata actgcctagc accagaatca tactggagtt
     6841 aacctggcgc tgaggcttag tcattgcggt gcctgtatca atgctgcgta caccatcaac
     6901 attgaaaaaa tactcgtaca ggttgccttt caggataggt gtgcgccacg accagactcc
     6961 tgcttcgtct ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca ccactacaga
     7021 gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg cattgacctg
     7081 agtcacatac tgtttcaccg ggatagtggg tgccgggctg gctggcatat ccgctgctat
     7141 ggcccactga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa tttttatatt
     7201 catcgtaata ttcctttcat taagttccat taccaccaga tttcagcctg ggcaccgaca
     7261 gcaaactggt cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt ttccagggct
     7321 ttgatatacg tggcgtagaa acgaatttcc agacgcgagg tcaacatact ggtattgact
     7381 ttaaaggtat ggaagagcgt ggttttataa ccagactcat taaatttatt actattcgca
     7441 tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata ttggtcccaa
     7501 atataggccg ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg
     7561 tagctataaa tatcgttacc gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg
     7621 ccgatatagg cttcgccctg cgaggtcaga cgaactgctg ttccgccggt gtgatcacca
     7681 taataacgac cattaaaggt ggtaaatgga cttgcgccag cataacggcc aaagttcctg
     7741 gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccttt atcaaatttc
     7801 tgcgttaaag atgtgccgaa catccaggta tctttccagt cataataccc gttattatcc
     7861 tgattatctt tttccgatgc gctttcgttt gccgtgacat aacgaccact caccattaag
     7921 gtcgctttat cccataacgg gatatcttta tagcgtaaat caatagtatg ggtattaatc
     7981 tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata
     8041 tcaattttac ccggaccgac tttccagttt tccagagcta cacccgctgc ggcatcagta
     8101 cgctgcgttt tccagtccag catctggatt tcaattttcg gcgcaccgtg tttacccacc
     8161 cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacgta catatcggag
     8221 aactgcataa agttctcgcc accggcgtta tcgccaaacc agccggtaga gtactgctga
     8281 ccaacgttac catccatcat cacaacggca tcaacccgtt tgccgttttc gttgtagaca
     8341 cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg gcccagagaa
     8401 ccaatcgccc atgatttagg tgaaccatga gaggcggtcc cccaaccaga acggtaatag
     8461 ccgttgtaac taaagccaat ttcatctttc acaaacttac tgaaatcttt cagagtcatg
     8521 gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttgac cagtacagca
     8581 tcaggttttg cggcactact ggtcgggaac ggattggcgg catacccttg atcattcgta
     8641 cttacgctac gattcaccgt cgctggcgta tattttttct tctcttcatc tttatacttt
     8701 ttgagttcgc tttgcgtttc tcttaacgct ttttctaata actcaaggcg ttgttccacc
     8761 gttaatgatt cagccaatga ttgtgcagaa aaggctaacg gtgccattaa taagatggca
     8821 gaggtaataa gatttcgtct aaacataata tccctttatg gtgcaaagaa agaattaacg
     8881 tatcgcatcc agactgttct gaatgcgacg ataattaagg tgctttattg gttatttttt
     8941 taatgacagc ccccgcgttt tgatcacttc tgcataccat ccgaagcttt tcttacgcgt
     9001 tcttgtgagg cttccttcgc cattatcatc acgatccaca taaataaagc cgtagcgctt
     9061 agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg tgtaccccat
     9121 aatatccaca ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc
     9181 aattcgataa tcatcctgta tcgaaccatc cgcttcaacg ctgtctttta cgcctaatcc
     9241 attctcgaca ataaataacg gtttttgata acgatcccaa agcgtattta acagaacccg
     9301 taatccaacc ggatcaattt gccaccccct ctctgaactt ttcagatgcg gattggggat
     9361 catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg aaacacaacc
     9421 agtcatgtaa taactaaaag agatgaaatc tacagtatgt tttaaatctt ctgcgtcact
     9481 ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat agccgggata
     9541 ctggccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt ccatggcctg
     9601 caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc
     9661 gattttcgct tcggggatca ggctatgata ggctttaact gcccgcgcac tggcaaccag
     9721 ttgatgatgg atcgcctgat aaacttccgc ctcgccactc tcttctgcca gccccacgcc
     9781 cgtgaatggc gcgtgtaacg acatattgat ttcattaaac gtcagccata acgctacttt
     9841 atgttggtag cgggtaaaga ccgtgcgggc gtaatgctcg aagtgatcga tgaccgcccg
     9901 attagcccaa ccgccgtagt ttttcaccag cccatatagc atttcgtaat gggataacgt
     9961 taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa
    10021 ccccgcttca ttcggttcgg cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga
    10081 aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg ggtaacggtg
    10141 ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg gttccatttt
    10201 tcccattacg ccatgaggct gtaaatctga ggtcgtgatc cctttgccat cttccagcca
    10261 ggcaccttcc acctgattgg cagctgttgc gccaccccaa agaaatgttt ctggaaatgc
    10321 tttcgtaatt aactcctttt atcgttagcg aatgatggat aacagcggtt cacctgcgct
    10381 tatctgcgcc gtgccgtggg gtaatacgtc tgtaaaatca tcgctattac tgattaatac
    10441 cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag aaatcagccg
    10501 atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag
    10561 ttttacggtg tcgataccga catgaatcag gatctccaca ccatcatctg actcaatgcc
    10621 aatggcgtgt aatgtggcga acaacgaagc aattcgaccc gcaaccggag aacgcacttc
    10681 accaaccgag ggcagaatgg cgataccttt acccagcagg ccactggcaa acgtggtatc
    10741 agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac cgccctgctc
    10801 aggtggtgta ataacctctg gtgttttctc ttgcggggca ccctgcgctg gctaacgttt
    10861 actggtgatg aaatgaagca tcaacgtacc aacaaatgcg caacctatgg caatgacacc
    10921 gccaataacg ctggcccaga cggtgaaatc aatccccgtt gacgggatgg tctgcatgaa
    10981 ggtgaaaata cttggcaaac caaaggagta aactttcgtt tgcgcgtagc caatgatggt
    11041 ggcccccaat gctccactga tacaggcgat aacaaagggg tacttacgcg gcaggttgac
    11101 gccatatacc gctggttcgg tgataccaaa cagacccgtc aacgccgctg atcccgccac
    11161 cacttttttc tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc cgacctgcgc
    11221 cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt
    11281 gatacacagc ggcaccaggc cccagtgcag tccgaacatg acgaagattt gccagaagcc
    11341 gcccattacc gcgcccgcaa atgcaggaac cgcctgataa agccagagat aaccggcggc
    11401 aatcatttcg cttatccagg ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat
    11461 aaccatcaga catagcaatg gtgtgaagaa atttttgatt gccgacggta accacgcatt
    11521 aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa taaccgatga
    11581 cgagtaattc aacaatgtga ccggaatacc caggaaatcc agccccggcg catccgcttt
    11641 ttgcccgttc tcgaaggcag tcagaattaa tggatgcact aacgctccac caatcaccat
    11701 ggcagtaaat ggattaccgc cgaagcgttt gcccgcggtg tatcccagga ttatcgggaa
    11761 gaacccaaac aaggcatcac tggcgctgaa taaaattaaa taagtaccac tttgttcggt
    11821 cgtccactga aaagtgagcg ccagagccag catacctttc aggatcccgg ttgccgccat
    11881 cagaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt ttagcagatt
    11941 acctttctca tcattttccg gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt
    12001 aaccgccagg aagacatcgg ccacatggtt acctatgacc acctgaaact gaccaccact
    12061 ttccaccacc ataataatac cgggggtctt tttcagtacc tcagcttgcg ctttgctttc
    12121 atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc agactcacaa tgttatctgc
    12181 gcccccgact cctgcgacta tttttctggc taactccgtc ataacttgcc ctctaccgct
    12241 ttgcggcaaa actccaaaaa aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt
    12301 ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ctcagtaata atatttcagt
    12361 gttctttgcg cacgcgctct atatttatgg ctaaacacat aatctctgcg ggtgaaattt
    12421 tacgttgata ctgtaaacca ataaaaatgg cgatccgttc cgcacattgc catgcttgcg
    12481 ggtaattttg ttttactgct tgttgtaatg attcatcact atcgttaatt gaagcatgtt
    12541 caagaatacg ccaggataaa aacttcagat gtgtaaccag tcgctgataa ctcaagcttt
    12601 cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttcg cgcattaact
    12661 gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc agatgcatgg
    12721 caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca ataatggtta
    12781 acgcttcttc ccctagctgg aactctttcg ggtaaagccg ctggatatcc cacagcaacg
    12841 ggttgggcag caacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
    12901 gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataagac
    12961 gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt
    13021 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
    13081 cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
    13141 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
    13201 ccttttttat aaacaaaaaa acccgacttc accagtattc tctggttatg tcaggttttg
    13261 cctgcgaatg cagtaacaat ccagtcattt attaatggtt tttataacga acatccaggt
    13321 tcggaaatta atttaattgc gtgcagagaa aatagcaatg cgctattgat aaaaatatga
    13381 ccatgctcgc agttattaac tttgtgtaat tttaggaatt tataaagtta tatataacaa
    13441 atcccaataa ttaagttatt gggatttgtc tggtgaatta tttgtcgcta tcttttcccg
    13501 ccagcagttt atccagctca tcactgccta catgacggaa atcctgaccc ttcacgtagt
    13561 agaagataaa ctcacaaata ttctggcagc ggtcgccgat acgttcgatt gaacgcgcac
    13621 agaacagcgc ggtaagtacg ctcggaatag tgcgcgaatc ttccatcatg taggtcatca
    13681 gttgacgaac aataccttcg tattcctgat cgactttttt atcttcacga taaatacgta
    13741 ccgcttcgtc aatgtccatc cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg
    13801 tatgacggcc cagcgactcc agacttacca gcaacggctg atgctgctgg gagaatttct
    13861 ccagcgcagt acggcagatt ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg
    13921 ttttactgat caccataacc agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa
    13981 tgcgcacgca ggcttcatcg atcgccactt ccatcatgtt gacgttcttg tcgccttcga
    14041 tcacgcgctt cgccagatcg ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct
    14101 gctgctccac catgccgccc atggtcatca cctgcgtgcg gatactttcc agttcggcgt
    14161 tgaactggcc ggaaatatgt ttattaagat tgagactgtc cataacgcac tcctgaatca
    14221 accgtaacga ccggtgatgt agtcttctgt ttgtttcttc gctggcttgg tgaacagatc
    14281 gtccgtgttg ctgaactcaa tcaattcgcc caggtacata aatgccgtgt ggtcggaaca
    14341 acgcgcagcc tgctgcatgt tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc
    14401 ggtgatcagc tcttcaatac gcccggtaga gatggggtcg agcgccgaac acggttcgtc
    14461 gagcagcagc acttccgggc gaatggcgat accacgcgca atacacagac gctgttgctg
    14521 accaccagag agagagtaac cgctctggtg caatttatct ttggtttcgt tccacaatgc
    14581 ggctttggtc aatgcccact gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa
    14641 cagacgaacg ccaaaggcga tgttgtcgta gatggacatc gggaacggcg tcggtttctg
    14701 gaacaccatg cccactttcg cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt
    14761 gtcgccatca agcagaattt caccttccgc acgctgctcc gggtacagtt caaacatttt
    14821 gttgaaggta cgcagcagcg tcgatttacc gcagccggac ggcccgataa acgccgtaac
    14881 ctggtttttg gcgatatcca ggttgatgtt tttcagggca tggaatttgc cgtagtagaa
    14941 gttcaaatta cgaacctgaa ttttactcgg ggcagtttca accatactca ttgcaatctt
    15001 ttcctcatcg tacctgatgc ttttcgctaa tcaggcttac cgtagggccg ggtaaggcgt
    15061 tcacgccgca tccggcaaag tgttaaccgt gtttattctt cgcaaaaaca acgcgcgcca
    15121 gaatgttcag cagcaggacg cacagggtaa tgatcaatac cccggcccag gccagttgtt
    15181 gccattccgc aaacgggctc atcgcaaact taaagatcgt caccggcagg ttggcgatcg
    15241 gctgcatcat gtccgtgctc cagaactggt tggagagcgc ggtaaacagc agcggcgcgg
    15301 tttcaccggc aatacgggca atcgccagca ggataccggt cataatcccg gacaccgacg
    15361 ctttcagcgt aatcgcggag atcatcttcc acttcggtgt acccagcgca taagccgctt
    15421 cacgcaggct gtccggcacc agtttcagca tgttctcggt ggtgcggata acgatcggca
    15481 cctgcaacag cgccagcgca attacgcccg cccagccgga gaagtgctcc atctgtgcca
    15541 ccacgatggt gtagacaaac agaccaacca caatcgacgg cgcggagagc agaatgtcgt
    15601 taatgaagcg aatcacttct gccagccagg atttacgacc atattccgcc agataaatcc
    15661 ccgccataat gcccagcggc gtaccgaata ccgtggccca caaaattaac agcccgctac
    15721 ccgccagggc gttcgccaga ccaccacctt ccgtattggg cggcggcgtc atttcagtga
    15781 acagcgccag cgacataccg tcgataccgc gagtgatggt ggacattaaa atccagatca
    15841 gccagaacag gccgaaggcc atcgtcgcca tcgagagcgt cagcgcaata cggtttttga
    15901 ggcggcgacg cgcctgcatt ttgcggcgag attcagccag cgccgcagtg gtttgcattt
    15961 caaccatagc cattagcgtg ccccctcatt cttagccagg cgcataatca taaacttcga
    16021 tgcggcgagg acgatgaagg taatcacaaa caggatcagg cccagttcca tcagtgcggc
    16081 aacgtgcaga ccggattccg cttccgcaaa ttcgttcgcc agcgcagagg tgatactatt
    16141 gcccggcatg tacagcgagg cgctgtcgag ctggtaggtg ttaccgatga taaaggtcac
    16201 cgccatggtt tcaccgagcg cgcggcccag ccccagcatg atgccgccga taacaccatt
    16261 tttggtgaac ggaagaacga tacgccagat aacttcccag gtggtgcagc caataccgta
    16321 ggccgactct ttcatcatca ccggggtttg ttcgaacaca tcacgcatta ccgccgcaat
    16381 gtacggaata atcatgatgg cgaggatcac gcctgccgcg aggataccga taccaaatgc
    16441 ggggccagag aacagcgcgc caacaatcgg gatattcgac atgatattgc cgaccggctc
    16501 ctgaaagtaa acggcgaaca gcggcgcaaa gataaacagg ccccacatgc cgtaaacgat
    16561 acttggaatg gctgccagca gctcaatggc gatacccagc gggcgtttca gccagccagg
    16621 cgcaagctca gtcaggaaca gggcgatacc gaaactcacc gggacggcga tcagcagcgc
    16681 gataaacgaa gtcaccaacg taccgtagat cggcaccagc gccccgtaga tatcgttcgg
    16741 tgcatcccac tctttggtcc ataggaaagc cagaccaaat ttctgaatgc tcggccagga
    16801 ggagatgatc agagagacaa taatgccacc caacatcaat agcacaatca gcgccgccag
    16861 ttttaccagc acgctgaaaa ttatgtcgcc ctttttaccc ggtgggttaa aagcgggctt
    16921 ggttgcagcc ataagttact cttcagttaa acgcgtttac aaagtcattt tgtaatgccg
    16981 gatgcggcgt aaaacaccgt acccggcctg gagttttatt agtacagcgg cttaccgcta
    17041 ctgtctttaa tattggtctt ccacgcagcg cgaacctgtt caactacact atccggcagg
    17101 ctagcgtaat ccaggtcgtt cgcctgttta gccccggttt tgtacgccca gtcgaagaat
    17161 ttcagcacct ctgtgccttg ttctggtttc ttctgatctt tgtggatcag aatgaacgtg
    17221 gtagaggtaa taggccatgc atcttcgcct ttctggttgg tcagatcctg agcgaaggtt
    17281 ttgctccagt ctgcaccttt tgctgcatta gcgaagtttt cttcggtcgg actaaccggt
    17341 ttaccatcag cggagatcag tttggtgtac gccaggttgt tctgcttcgc gtaagcatat
    17401 tcaacataac caattgcacc cggcagacgc tgaacgaacg cggcgatacc gtcgttacct
    17461 ttaccgccca gaccgatcgg ccattttacg gtagagccag taccaacgtt gtttttccac
    17521 tcttcgttca ctttcgccag gtagctggtg aagacgaagg aagtcccgga gccatctgcg
    17581 cggcgtacta cagcaatgtt ttgtgaaggc agtttcagac ccggattcag tttggcgatg
    17641 gcttcatcat cccacttctt gattttgcct aggtagatgt cgccgagggt tttaccatcc
    17701 agcaccagtt cgccagactt cagccctgga atgttaaccg ccagcaccac gccgccaatc
    17761 acggtcggga actggaacag accttcctga gccagttttt cgtcagacag cggcgcgtca
    17821 gaggcaccaa aatcaacggt attagcgata atctgtttta cgccaccgga agaaccgata
    17881 ccctggtagt taactttatt accggtttct ttctggtaag tatcagccca tttggcatac
    17941 accggcgcag ggaaggttgc acctgcacct gtcaggcttg cttctgcaaa cacagagaaa
    18001 gcactcatcg ataaggtcgc ggcgacaaca gttgcgacgg tggtacgcat aactttcata
    18061 atgtctcctg ggaggagtca taaagcattg tttgttggct acgagaagca aaataggaca
    18121 aacaggtgac agttatatat aaggaatatg acatttttat gacagaaagg aattaccaaa
    18181 taacttactg attttaaatg tgaagatgaa aatcccacat gccatctttt tgtcatgatg
    18241 catttaaaag tgataaagaa atgggtgaga tgacaacgat gtgatgaaca atcgttgtca
    18301 gacctattag cgcacaatct caacagttat cgtcgctgtt gcagtaaatt gccccggggc
    18361 tgggcgagct ccagtaaggc tcgcaggagc agctgaaaaa gttacactac ctgatttttc
    18421 accatacact gttgatttat ccatgtctgt tggcaactca ccaccgttca ctttgatttc
    18481 cttattattt ttgtcgtaaa tttttatccc cacgttagag tttgaagtta cgaccattga
    18541 attgtccgta ttgggttcgg tagcataaaa agacgtactg atatcatcgg tattcagtat
    18601 ccctaggttt tcacactcaa tagtgactgt tttagtaatt ttatgtgttg ttaccgcact
    18661 tccaacaaca gatgaaaaat ctgcagctgg tatttcttta aaatcaaaac aaactggctt
    18721 taaattattt atttcgcaac tttgcggagc tgtaatatcg ccccgaatat aaacatcagc
    18781 catcggctgt gagtaatcca ccggggtgtc acggctaatt gttccataca atgtcactat
    18841 ctttgtttca ggaataatca attttcctgc aaatgtcttc ttgatataaa aatatactat
    18901 cgctttagcc ccactggata tacttaaagg ttcaatgcgg ttgcaattgt actttgtatt
    18961 accagacggg ttgttagcat gatattcaaa aggtgcattc acatacccaa ccccaagaat
    19021 atctatagca atcccaacat caaggttcgg attaagatca taataattaa tattagagtg
    19081 aaccccagca taagttaatg cctggtttat ttttgacgta taatagatat aggggaattc
    19141 tcctacgtta ccagtatcac aatgacagcg tccaagatat gaggatccat tagttacagt
    19201 ggtgttaaat gtcgttccaa ccttattttc gttcgcggta ataagatatc ccgagaaatc
    19261 aagcgtactg tgaaatacgc cattatcagg ggtacagata cctttaggag ctgcaagtgc
    19321 tacactactt aacgagagca taaacacaga aaagcaccac tgtttgatat acttgttcat
    19381 aaaaatcctt ttaatgacat gtcacaccct gttcaatt
//
LOCUS       DADLZM010000075        19376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLZM010000075 DADLZM010000000
VERSION     DADLZM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.075"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(30..1859)
                     /gene="glmS"
                     /locus_tag="JNY73_002459"
     CDS             complement(30..1859)
                     /gene="glmS"
                     /locus_tag="JNY73_002459"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY8048442.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2021..3391)
                     /gene="glmU"
                     /locus_tag="JNY73_002460"
     CDS             complement(2021..3391)
                     /gene="glmU"
                     /locus_tag="JNY73_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY8048443.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGPLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JNY73_002461"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JNY73_002461"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY8048444.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JNY73_002462"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JNY73_002462"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY8048445.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JNY73_002463"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JNY73_002463"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY8048446.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JNY73_002464"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JNY73_002464"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY8048447.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JNY73_002465"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JNY73_002465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY8048448.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JNY73_002466"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JNY73_002466"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY8048449.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JNY73_002467"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JNY73_002467"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY8048450.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JNY73_002468"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JNY73_002468"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY8048451.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JNY73_002469"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JNY73_002469"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY8048452.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JNY73_002470"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JNY73_002470"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY8048453.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JNY73_002471"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JNY73_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY8048454.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JNY73_002472"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JNY73_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY8048455.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JNY73_002473"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JNY73_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY8048456.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="JNY73_002474"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="JNY73_002474"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY8048457.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JNY73_002475"
                     /pseudo
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JNY73_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JNY73_002476"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JNY73_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY8048458.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLKASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4628 a         5119 c         4860 g         4769 t
ORIGIN      
        1 cggcagccct tttttatcta tgatagtgat tactcaaccg taaccgattt tgccaggtta
       61 cgcggctggt caacgtcggt gcctttgatc agcgcgacat ggtaagccag cagctgcagc
      121 ggaacggtgt agaagatcgg tgcaatcacc tcttccacat gcggcatctc gatgatgtgc
      181 atgttatcgc tacttacaaa acccgcatcc tgatcggcga agacatacaa ttgaccgcca
      241 cgcgcgcgaa cttcttcaat gttggatttc agtttttcca acagttcgtt gttcggtgca
      301 acgacgataa ccggcatatc cgcgtcaatc agcgccagcg ggccgtgttt cagctcgcct
      361 gcagcgtagg cttcagcgtg aatgtaagag atctctttca gcttcaatgc gccttccagc
      421 gcgattgggt actgatcgcc acggcccagg aacagcgcgt gatgtttgtc agagaaatct
      481 tctgccagag cttcaatgcg tttatcctga gacagcatct gctcaatacg gctcggcagc
      541 gcctgcagac catgcacaat gtcatgttca atggaggcat ccagaccttt caggcgagac
      601 agcttcgcca ccagcatcaa cagcacagtt aactgagtgg tgaatgcttt agtggacgcc
      661 acgccgattt ccgtacccgc gttggtcatc agcgccagat cggattcgcg caccagagaa
      721 gaacccggaa cgttacagat tgccagcgaa ccaaggtaac ccagctcttt cgacagacgc
      781 aggcccgcca gggtatccgc ggtttcgcca gactgtgaca aggtgatcat caggctgtta
      841 cgacgcacgg cagatttacg atagcggaat tcagaggcga tttcgacgtc gcacggaata
      901 cctgccagcg attcaaacca gtagcgggaa accataccgg agttataaga agtaccacag
      961 gcgaggatct gaatatgctc aaccttcgac agcagttcgt cggcgttcgg tcccagctcg
     1021 cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg tgttttttat cgcgttcggc
     1081 tgttcgtaga tctctttctg catgtagtga cggtaaatac ctttatcgcc cgcgtcatat
     1141 tgcagattgg attcgatatc ctgacgtttt acttccgcgc cagttttatc gaagatgttt
     1201 accgaacggc gagtgatttc cgcaatatcg ccctcttcaa ggaagataaa gcgacgggtc
     1261 accggcaaca gcgccagctg gtcagaagcg ataaagtttt cgcccatacc caggccaatc
     1321 accagcggac taccagaacg tgccgccagc agggtatccg ggtgacggga gtccatgatc
     1381 actgtaccgt acgcaccgcg cagctgcggg atagcacgca gaacagcctc acgcagagtc
     1441 ccgccttgtt tcagctccca gttcaccaga tgggcaatca cttcggtgtc ggtttcagaa
     1501 acgaaggtat agccacgcgc ttttaactct tcacgcagcg gttcatggtt ttcgatgatg
     1561 ccattatgca ccaccacaat gtgttcagaa acatgcggat gcgcgttagc ttctgaaggt
     1621 tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc cgccatgcag aggatgttct
     1681 tccgctgcct gagccagcat ctggacttta ccgaggcgac gcaggcgagt catatgacct
     1741 tccgcatcaa caacggccag accggcagag tcatatccgc ggtattccag acgacgtaaa
     1801 ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg cgccaacaat tccacacata
     1861 gtttttgatt ccgatttata tcgttgtcgg tcaacctgta tgcccgtttt ttcgggcgcc
     1921 ccgagccttg tagagagtgg ggttattttt atagttactg cttgtgggag ggagattatg
     1981 ttatctcctc atcccagatg accggattac ccggccagaa ttactttttc tttaccggac
     2041 gacgccagcc ttctttctga gtctgcggca cacggctgat agctaatgca ttttcgccga
     2101 cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc tttgcctact gttaccgggg
     2161 ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc gataatggtc ttaaatttat
     2221 tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat gttaacgtta tcgccaattt
     2281 ccgcatcgcc taggtaagtc agatgcccgg ctttcgtgcc tttacccaga cgcgcttttt
     2341 tcatctcaac gaagttacca acgtgtgcac cttccagcaa ctcagcacca ggacgcagac
     2401 gggcaaacgg gccaatagta caggccgctg ccagattcgc gtcttccaca acggtatacg
     2461 gactgatttc gcaatcatcg ccaatcacgc tgtttttaat cacgcaaccg gtgccgattt
     2521 tcacgcgatg accgagagtc acgttgccct cgatgataac gttagtatca atttcaacat
     2581 cgcgcccgtg agtaagcggg ccgcgcagat caaaacgcgc cggatcgcgc agcataacgc
     2641 ctgctaacag cagtttttca gcctgttcgg actggtaaac gcgctccaga cgggagagtt
     2701 gcaggcggtt attcacacct tctacttcgc ttaaacgttg cggatgaacg gcgacgattt
     2761 cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat gtagtattcg ccctgagcat
     2821 tattgttggt cagcttcgcc agccagcgtt tcatatctgc gccgttggca atcagaatgc
     2881 cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc atctttgtgc tcaacaatgc
     2941 cggtaacttt gccattttca cgggtgatac gtccataacc ggtcggatca tccagtttca
     3001 ccgtcagcag accaatgcca ccctggggtt tagcatcacg caggcgctgg agtgtttcga
     3061 cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc ttcatcatcg gcaaagaaag
     3121 gtgcggcctg ctgcattgca tgacccgtac ccagctgctc tgcctgaagc acccagttaa
     3181 ggttgtcgtc tttcaacgcc tgtttaagca gatcgccgcc gtgaccgtac accaggtgaa
     3241 cgtgcgctgc gcctaattca ttcgcagcat caatgacatg ctgaaccatc gctttcccgg
     3301 caagggtatg cagcactttc ggaagatcgg aatacatgcg cgtgcctttg cctgcggcaa
     3361 ggatcactac gctcatagca ttattcaaca tacgcgtcct gactgtaatt tgagaacgaa
     3421 tttaaaccgc ttcaccttga aaaaactaca tttttttcat cgtgaaatgg acagaggata
     3481 aattgttcaa taacggatta tccccgctga cgaaagtgcc attttcgacc atcgtcacgc
     3541 cttttgtctg cagaaaatta aggcagtacc ttcatttaag cgtcaggtgg atgtttttgc
     3601 tcttatttcg atcaatgaat aaacgggaaa acaggggtgt tttatatcag caggatctat
     3661 gtgaacgctc ttcaggacgg gtcaacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacttg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagggcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggagtttat tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgttg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcaag tcgataggca gtaaatccat cagattcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caagaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaagccac ccggcctttc gctggtgtat
    10441 gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct acccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatca
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtct
    12961 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgca gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacgctaaa gtcgatggtt
    13201 cgagcatcaa tacgcggcgg agtaccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagagagcgg aatggatggc ggatcgccag cacggccacc gctgtaatta
    13321 tccagaccga tatgaatttt gccgtcgagg aacgtcccaa cggtgagcac cacagctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagcgcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cagccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactgccc tgtggataac aaggatccga cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aacggttgtt ctttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctgccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata accagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatggta cgcatgattg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat aacttttata aagatgctgt
    14881 agtggccggt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggtttt ttattgaatg attattgcat gtgtgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttaac gaagagtttg gcctggcacc gttcctgccg gatcagattc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat tgcgaaagat cttggcgcgg tattcctcgt cgggattggc ggcaagttga
    15961 gtgatggtca tcgtcacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggcca tgcgggcctg aacggcgata ttctggtgtg gaacccggta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgctgaag catcagctgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gagtatggcc agttgctgtt cgcgagagcg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gctacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatataa cagcgccggt tttcagcgag
    16741 agcaatgcgc atcaaagcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga gcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctacgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaata aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtctagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag tcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgctta cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgtac aacaagatgt tgggtgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gcagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt aacgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgcccaggc gggtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcaa ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc cttcaataag cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ttgcacttta tctaaccata gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagtaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacgatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gagtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg ggtaat
//
LOCUS       DADLZM010000076        18261 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLZM010000076 DADLZM010000000
VERSION     DADLZM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.076"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="JNY73_002477"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="JNY73_002477"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY8048459.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="JNY73_002478"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="JNY73_002478"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY8048460.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..2943)
                     /locus_tag="JNY73_002479"
     CDS             complement(1729..2943)
                     /locus_tag="JNY73_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048461.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLAEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVRFGERK
                     FSTRLNTIKHRG"
     gene            complement(3183..4328)
                     /locus_tag="JNY73_002480"
     CDS             complement(3183..4328)
                     /locus_tag="JNY73_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048462.1"
                     /translation="MGVILMSLNMASLETLCQTNAAGVSIVISSLGIGRLSVLLFAGL
                     LSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFGFLAGMANSFLDAGTYPSLME
                     AFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAAGIMFINALFLYR
                     CTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMATFYLVSQWLAQYGQFV
                     AGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLMLYTFISFIALFTVCLHP
                     TFYVVIIFAFVIGFTSAGGVVQIGLTLIAERFPYAKGKATGIYYSAGSIATFTIPLIT
                     AHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHSLKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="JNY73_002481"
                     /pseudo
     CDS             complement(4487..4843)
                     /locus_tag="JNY73_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="JNY73_002482"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JNY73_002482"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JNY73_002483"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JNY73_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY8048463.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="JNY73_002484"
     CDS             6773..9829
                     /locus_tag="JNY73_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY8048464.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="JNY73_002485"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="JNY73_002485"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY8048465.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="JNY73_002486"
     CDS             10336..10524
                     /locus_tag="JNY73_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048466.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="JNY73_002487"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="JNY73_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY8048467.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="JNY73_002488"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="JNY73_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY8048468.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="JNY73_002489"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="JNY73_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY8048469.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="JNY73_002490"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="JNY73_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY8048470.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTVHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGFKQQVLARIAQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="JNY73_002491"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="JNY73_002491"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY8048471.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="JNY73_002492"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="JNY73_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY8048472.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="JNY73_002493"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="JNY73_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY8048473.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /locus_tag="JNY73_002494"
     CDS             complement(17801..18037)
                     /locus_tag="JNY73_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY8048474.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4804 a         4405 c         4690 g         4362 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccagcca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcttgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgaacg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagctatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc
     4141 atatagcagc acattccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgaca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaataaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttactgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcagcg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg gacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgtccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gctagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggcctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gcatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaaatcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac tttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagtt atcgcggcca ggaaacgcac gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gtccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gtttaaacag caggtgctgg cccgaatcgc tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg gttgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca gcgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcggcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt g
//
LOCUS       DADLZM010000077        18180 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLZM010000077 DADLZM010000000
VERSION     DADLZM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.077"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..454
                     /locus_tag="JNY73_002495"
                     /pseudo
     CDS             1..454
                     /locus_tag="JNY73_002495"
                     /inference="COORDINATES: protein motif:HMM:NF013106.1"
                     /note="penicillin-insensitive
                     transglycosylase/transpeptidase; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            941..2090
                     /locus_tag="JNY73_002496"
                     /pseudo
     CDS             941..2090
                     /locus_tag="JNY73_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            2240..2671
                     /gene="ndk"
                     /locus_tag="JNY73_002497"
     CDS             2240..2671
                     /gene="ndk"
                     /locus_tag="JNY73_002497"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY8048475.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2821..3975
                     /gene="trmG/rlmN"
                     /locus_tag="JNY73_002498"
     CDS             2821..3975
                     /gene="trmG/rlmN"
                     /locus_tag="JNY73_002498"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY8048476.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4260..5273
                     /gene="rodZ"
                     /locus_tag="JNY73_002499"
     CDS             4260..5273
                     /gene="rodZ"
                     /locus_tag="JNY73_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY8048477.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5300..6418
                     /gene="ispG"
                     /locus_tag="JNY73_002500"
                     /gene_synonym="gcpE"
     CDS             5300..6418
                     /gene="ispG"
                     /locus_tag="JNY73_002500"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY8048478.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6529..7803
                     /gene="hisS"
                     /locus_tag="JNY73_002501"
     CDS             6529..7803
                     /gene="hisS"
                     /locus_tag="JNY73_002501"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY8048479.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7821..8441
                     /locus_tag="JNY73_002502"
     CDS             7821..8441
                     /locus_tag="JNY73_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY8048480.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8452..9630
                     /gene="bamB"
                     /locus_tag="JNY73_002503"
     CDS             8452..9630
                     /gene="bamB"
                     /locus_tag="JNY73_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY8048481.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9748..11220
                     /gene="der"
                     /locus_tag="JNY73_002504"
     CDS             9748..11220
                     /gene="der"
                     /locus_tag="JNY73_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY8048482.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11290..11505
                     /locus_tag="JNY73_002505"
     CDS             11290..11505
                     /locus_tag="JNY73_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048483.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11502..12872)
                     /gene="xseA"
                     /locus_tag="JNY73_002506"
     CDS             complement(11502..12872)
                     /gene="xseA"
                     /locus_tag="JNY73_002506"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY8048484.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13034..14500
                     /gene="guaB"
                     /locus_tag="JNY73_002507"
     CDS             13034..14500
                     /gene="guaB"
                     /locus_tag="JNY73_002507"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY8048485.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14569..16146
                     /gene="guaA"
                     /locus_tag="JNY73_002508"
     CDS             14569..16146
                     /gene="guaA"
                     /locus_tag="JNY73_002508"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY8048486.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKTDLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16241..16780)
                     /locus_tag="JNY73_002509"
     CDS             complement(16241..16780)
                     /locus_tag="JNY73_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048487.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16796..17314)
                     /locus_tag="JNY73_002510"
     CDS             complement(16796..17314)
                     /locus_tag="JNY73_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY8048488.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17625..17816)
                     /locus_tag="JNY73_002511"
     CDS             complement(17625..17816)
                     /locus_tag="JNY73_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY8048489.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17834..17986
                     /locus_tag="JNY73_002512"
     CDS             17834..17986
                     /locus_tag="JNY73_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048490.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4300 a         4453 c         4994 g         4433 t
ORIGIN      
        1 ttaccttgaa gcgctgatca attatgaaga tcgctggttc tggaagcatc cgggggttaa
       61 tccattctcg gtggcgcgcg cagcatggca agatctcact tcgggacggg ttatttccgg
      121 tggcagcacg ctcactatgc aggttgctcg tctgcttgat cctcacccca aaacatttgg
      181 tggcaaaatt ctccagctct ggcgcgcgtt gcaactggag tggcatttgt ctaagcgtga
      241 aattctgatc ttgtatctta accgcgctcc gtttggcggt acgttgcagg ggatcggtac
      301 ggcaagttgg gcttatctcg gaaaatcgcc tgcgaattta agctattccg aggcggcaat
      361 gctggcggtg ttaccgcaag tgctcagccg actgcgcccg gatcgttggc cggagcgtgc
      421 cgaagccgcg cgtaataaag tgctcgaacg gatggctgcg caaggtgtgt ggtcccgtga
      481 gcaggtaaaa gagtcaaggg aagaacccat ctggctggca ccccgacaaa tgccgcaact
      541 ggcaccgctg ttttcgcgca tgatgctcgg taaaagcaaa agcgacaaaa tcgttactac
      601 gctggatgcc ggtcttcaac gacgtctgga agaactggcg caaaactgga aagggcggtt
      661 gccaccgcgc agctcactgg cgatgatcgt ggttaatcat accgatatgc gtgttcgcgg
      721 ctgggtggga tcggttgatc tcaacgatga ttcacgcttt ggtcatgttg atatggtcaa
      781 tgcgatccga tcgccaggat cggtgctcaa accgtttgtt tatggtctgg cgctggatga
      841 aggcttgatc cacccggcat cactgctgca agacgtcccc cggcgcaccg gtgattatcg
      901 accaggtaac tttgatagcg gttttcatgg cccgatcaac atgagcgagg cgctggtgcg
      961 ctcgctgaac ttaccagctg tgcaggtgct ggaagcctat ggaccgaaac ggtttgcggc
     1021 aaagttacgc aatgttggat tgccgttata tttgcccaac ggcgctgcgc cgaatctttc
     1081 actcattctc ggcggcgctg gtgcaaaact ggaagatatg gcggcagcgt ataccgcgtt
     1141 tgctcgccac ggcaaggcag gcaaattgcg cttacagcct gacgatccgc tgcttgaacg
     1201 gcctttaatg tcgtcggggg cggcgtggat cattcgccgg attatggctg atgaagcgca
     1261 acccttgccg gatagtgcct tgccgcgcgt cgccccactg gcatggaaaa cgggcaccag
     1321 ctatggctat cgtgacgcct gggcgattgg ggttaacgct cgctatgtca ttgggatctg
     1381 gactggcaga cctgacggca cgcccgttgt tggtcagttt ggctttgcca gtgccgtacc
     1441 attagttgaa tcaggtcaat aatatcttac tgtcgcgcag tgcgaatctg ccggaagacc
     1501 cgcgcccgaa ctcagtcact cgtggcgtta tctgctggcc gggcggacag tctttgccgg
     1561 aaggtgacgg taactgtcgc cgccgcctgg caacctggct gctggatggc agccagccgc
     1621 caacgctgtt actgtcagag caggaaggga ttaatggcat tcgcttcccc atctggctgg
     1681 atgacaaagg gcagcgcgtc gccgctgact tcccgcaggc gaagcaagaa atggtaaatg
     1741 tctggccgct accgctggaa ccctggctgc ccgcatcaga acgtcgcgct gtgcgcttac
     1801 caccggcctc gacaatttgc ccgccatacg gtcacgatgc ccagctccct ctgcagctga
     1861 caggcgttcg cgatggcgcg attattaaac gtttaccggg cgcagcggaa gcgactttgc
     1921 cgttgcaatc aagtggaggg gcaggtgaac gctggtggtt tctgaatggc gaaccgttaa
     1981 ctgaacgcgg gcgcaacgtg accctgcatt tgacggataa aggtgattat caattgctgg
     2041 tgatggatga tgtggggcaa atcgcgacag tgaaatttgt catgcaatag tctgttttgt
     2101 ccctgattgt tgctaaaact cattttattt taaaaaaaat gttaccttca tcaatagtca
     2161 acggccctgt tgctcattat aatccgcgcc atctcgtacg ctggtacaga caacaacaga
     2221 acaatttaca gaggtaaaaa tggctattga acgtactttt tccatcatca aaccgaacgc
     2281 ggtagcaaaa aacgtcattg gtaatatctt tgcgcgcttt gaagctgcag ggttcaaaat
     2341 tgtcggcacc aaaatgctgc acctgaccgt tgaacaggca cgtggctttt atgctgaaca
     2401 cgatggaaaa ccgttctttg atggtctggt tgaattcatg acctctggcc cgatcgtggt
     2461 ttccgtgctg gaaggtgaaa acgccgttca gcgtcaccgc gatctgctgg gcgcgaccaa
     2521 tccggcaaac gcactggctg gtactctgcg cgctgattac gctgacagcc tgaccgaaaa
     2581 cggtacccac ggttctgatt ccgtcgaatc tgccgctcgc gaaatcgctt atttcttcgg
     2641 cgaaggcgaa gtgtgcccgc gcacccgtta ataatttcgt aaatgccgcg tgcaaacgtg
     2701 gcatccgtgc gccagaattt gtacaatgta gcgcccccgg acgagcagcc gctcaccggg
     2761 gcgtttcttt tttcaaccct ccaggggcca taacgtgtaa taacgaggcc ggaatagcat
     2821 atgtctgaac aattagtcac acctgaaaac gtcaccacga aagatggaaa aatcaacctg
     2881 ctggatctca accgtcagca gatgcgggag ttttttaaag atttaggtga aaaaaccttc
     2941 cgcgccgatc aggtgatgaa gtggatgtat cactattgct gcgacaactt tgatgagatg
     3001 accgacatca acaaagtgtt gcgcggcaaa ctgaaagagg tggcggaaat ccgcgcaccg
     3061 gaagtggttg aagaacagcg ttcatccgac ggcaccatta aatgggcgat cgccgttggc
     3121 gatcagcgcg tcgaaacggt gtatatcccg gaagacgacc gtgccacgct ctgcgtctct
     3181 tcgcaggtgg ggtgtgcgct ggagtgtaaa ttctgttcca ccgcccagca gggctttaac
     3241 cgcaacctgc gggtatcgga aattattggc caggtgtggc gtgcggcgaa aatcgtcggc
     3301 gcagcaaaag tcaccggtca gcgcccaatc actaacgtcg tgatgatggg catgggcgag
     3361 ccgctgctca acctgaacaa cgtcgttccg gcgatggaaa tcatgcttga tgatttcggt
     3421 tttggcctgt ctaaacgtcg cgtgacgctt tccacttccg gtgtagttcc ggcgctggat
     3481 aaactgggcg atatgatcga cgttgcactg gcaatttccc tgcacgcgcc gaacgacgaa
     3541 attcgtgacg aaattgtgcc gatcaacaaa aagtacaata tcgaaacgtt ccttgctgcg
     3601 gtgcgccgtt atctggagaa atccaacgcc aatcagggcc gagtcactat tgaatacgtg
     3661 atgcttgacc acgttaacga cggcactgaa cacgcgcacc aactggcgga actgctgaaa
     3721 gatacgccgt gtaagatcaa cctgatcccg tggaacccgt tcccgggcgc gccgtatgga
     3781 cgcagttcga acagccgtat cgaccgcttc tcaaaagtgc tgatgagcta cggtttcacc
     3841 actattgtgc gtaaaacgcg tggtgatgat atcgatgctg cctgtggtca gttggcgggc
     3901 gatgttatcg accgtacgaa acgtactctg cgtaaacgga tgcagggtga agccatcgat
     3961 attaaagcgg tctgatagcc attacgccac ggttacttta tgtaccgtgg cgtaatcttt
     4021 tatgaatgaa gtattaagtt gcaattgtct ggtcaatatt aacggtgcgt ttttgctgac
     4081 tttaaggcag tatgtaacga tgcaacagta agttagcctc agtgaatgtg ggctttgtca
     4141 cgagcacaca gacggtctta tactgtatga taacggttaa cttaacggat gtttcgcggt
     4201 gtgggtgagc atgatggttc accggcatct caattctcat ttaaacgtac ctgcagcgaa
     4261 tgaatactga agccacgcac gaccaaaatg aagcacttac taccggcgct cgcctgcgta
     4321 atgctcgcga acaactagga cttagtcagc aggccgttgc cgagcgactt tgcctgaagg
     4381 tttccacggt acgcgacatt gaagaagata aggcacccgc cgatcttgct tcaacattcc
     4441 tgcgcggata tatccgctct tatgcgcgtc tggtacatat tccagaagaa gaactgctgc
     4501 cagggctgga aaagcaggct ccacttcggg ctgcaaaagt tgcgccaatg cagagttttt
     4561 ccctcggtaa acgccgcaaa aaacgcgacg gctggctgat gaccttcacc tggctggtgt
     4621 tgtttgtggt tatcggcctg agcggtgcct ggtggtggca agaccacaaa gctcagcagg
     4681 aagagatcac cactatggcc gatcaatctt cggcggaact gagcagtaat agcgagcagg
     4741 ggcagagtgt tccgttaaat acgtcgacaa ctacagaccc ggctacaacg tccacgccgc
     4801 cagcgtctgt ggatactacc gcaaccaaca cgcaaacacc tgccgtaact gcgccagcac
     4861 cagctgttga tccgcaacag aatgcggttg tttcgccttc gcaggcaaat gttgataccg
     4921 ccgcgacccc ggtaccaacg gcagcaacaa cgccagatgg tgctgcgccg ttgccaaccg
     4981 atcaggctgg cgtgaccacg ccggcggctg atccgaatgc gctggtgatg aactttactg
     5041 ccgattgctg gctggaggtc actgatgcta ccggtaaaaa attgtttagc gggatgcagc
     5101 gtaaagacgg taatttgaat ttaaccggcc aggcaccgta taaactgaaa attggtgcgc
     5161 cagccgcagt acagatccag tatcaaggga aacctgtcga tctgagtcgt tttatcagaa
     5221 ctaaccaggt tgcgcgtctg accctcaatg ccgaacaatc accggcgcag taacagacgg
     5281 gtaacgcggg agatttttca tgcataacca ggctccaatt caacgtagaa aatcaacacg
     5341 tatttacgtt gggaatgtgc cgattggcga tggtgctccc atcgccgtac agtccatgac
     5401 caatacgcgt acgacagatg tcgaagcaac ggtcaatcaa atcaaggcgc tggaacgcgt
     5461 tggcgctgat atcgtccgtg tctccgtacc gacgatggac gcggcagaag cgttcaaact
     5521 catcaaacag cgggttaacg tgccgctggt ggctgacatc cacttcgact atcgcattgc
     5581 gctgaaagta gcggaatacg gcgtcgattg tctgcgtatt aaccctggta atatcggtaa
     5641 tgaagagcgt attcgcatgg tggttgactg tgcgcgcgat aaaaacattc cgatccgtat
     5701 tggcgttaac gccggatcgc tggaaaaaga tctgcaagaa aagtatggcg aaccgacgcc
     5761 gcaggcgttg ctggaatccg ccatgcgtca tgttgatcat ctcgatcgcc tgaacttcga
     5821 tcagttcaaa gtcagcgtga aagcgtctga cgtcttcctc gctgttgagt cttatcgttt
     5881 gctggcaaaa cagatcgatc agccgctgca tctggggatc accgaagccg gtggtgcacg
     5941 cagcggggca gtaaaatccg ccattggttt aggtctgctg ctgtcggaag gcatcggcga
     6001 cacgctgcgc gtatcgctgg cggccgatcc ggtcgaagag atcaaagtcg gtttcgatat
     6061 tttgaaatcg ctgcgtatcc gttcgcgagg gattaacttc atcgcctgcc cgacctgttc
     6121 gcgtcaggaa tttgatgtta tcggtacggt taacgcgctg gagcaacgcc tggaagatat
     6181 catcactccg atggacgttt cgattatcgg ctgcgtggtg aatggcccag gtgaggcgct
     6241 ggtttctaca ctcggcgtca ccggcggcaa caagaaaagc ggcctctatg aagatggcgt
     6301 gcgcaaagac cgtctggaca acaacgatat gatcgaccag ctggaagcac gcattcgtgc
     6361 gaaagccagt cagctggacg aagcgcgtcg aattgacgtt cagcaggttg aaaaataata
     6421 acgtgatggg aagcgcctcg cttcccgtgt atgattgaac ccgcatggct cccgaaacat
     6481 tgagggaagc gttgagggtt catttttata ttcagaaaga gaataaacgt ggcaaaaaac
     6541 attcaagcca ttcgcggcat gaacgattac ctgcctggcg aaacggccat ctggcagcgc
     6601 attgaaggca cactgaaaaa cgtgctcggc agctacggtt acagtgaaat ccgcttgccg
     6661 attgtagagc agaccccgct attcaaacgt gcgattggtg aagtcaccga cgtggttgaa
     6721 aaagagatgt acacctttga ggatcgcaat ggcgacagcc tgactctgcg ccctgaaggg
     6781 acggcgggct gtgtacgcgc cggcatcgag catggtcttc tgtacaatca ggaacagcgt
     6841 ctgtggtata tcgggccgat gttccgtcac gagcgtccgc agaaagggcg ttatcgtcag
     6901 ttccatcagt tgggctgcga agttttcggt ctgcaaggtc cggatatcga cgctgaactg
     6961 attatgctca ctgcccgctg gtggcgtgcg ctgggtatct tcgaacacgt aactcttgag
     7021 ctgaattcta tcggttcgct ggaagcacgc gccaattacc gcgatgcgct ggtggcattc
     7081 cttgagcagc ataaagaaaa gctggacgaa gactgcaaac gccgcatgta cactaacccg
     7141 ctgcgcgtgc tggattcaaa aaatccggaa gtgcaggcgc ttctcaacga cgctccggca
     7201 ttaggtgatt atctggacga ggaatctcgt gagcattttg ccggtctgtg caaactgctg
     7261 gagagcgcgg ggatcgctta caccgtaaac cagcgtctgg tgcgtggtct ggattactat
     7321 aaccgtaccg ttttcgagtg ggtgactaac agtctcggct cccagggcac cgtgtgtgca
     7381 ggcggtcgtt atgacggtct tgtggaacaa ctgggcggtc gtgcaacacc ggctgtcggt
     7441 tttgcgatgg gcctcgaacg tcttgtattg ttagtacagg ccgttaatcc ggaatttaaa
     7501 gccgatcctg ttgtcgatat atacctggtg gcttcaggtg ctgatacaca atctgcggct
     7561 atggcattag ctgagcgtct gcgtgatgaa ttactgggcg tgaaattgat gaccaaccac
     7621 ggcggcggca actttaagaa acagtttgcc cgtgctgata aatggggtgc ccgcgttgct
     7681 gtggtgctgg gtgagtctga agtggctaac ggcacagcag tagtgaagga tttgcgctct
     7741 ggtgagcaaa cggcagttgc gcaggatagc gtagccgcgc atttgcgcac gttactgggt
     7801 taaggaagga gaaggacagc gtggaaattt acgagaacga aaacgaccag gtagaagcgg
     7861 ttaaacgctt ttttgctgaa aatggcaaag cactggctgt tggggtgatt ttgggcgttg
     7921 gcgcactgat tggctggcgc tactggaaca gccatcaggt tgattctgca cgctccgctt
     7981 ctcttgccta tcaaaatgcg gttaccgcag tgagcgaagg caaaccggat agcatcccgg
     8041 cggcggaaaa atttgctgct gaaaataaaa atacttatgg tgcgctggct tctttggaac
     8101 ttgcgcagca atttgttgac aaaaatgaac tagagaaagc tgccgcccag ttacaacagg
     8161 ggctggcaga cacgagcgat gaaaatctca aagccgtgat aaatctgcgt cttgctcgcg
     8221 ttcaggtaca gctcaagcag gctgatgccg cgctgaaaac ccttgatacc atcaaaggtg
     8281 aagggtgggc tgccattgtt gccgacctgc gtggtgaagc attgctgagc aaaggtgata
     8341 agcaaggtgc gcgtagtgca tgggaagcag gcgtgaaaag cgatgttact ccggcactga
     8401 gcgaaatgat gcagatgaaa attaataatt tgtccatctg agagggaccc gatgcaattg
     8461 cgtaaattac tgctgccagg actgctttcc gttacccttt taagcggctg ttcgctgttt
     8521 aacagcgaag aagatgtggt aaagatgtcc ccattgccaa ccgttgaaaa ccagtttacg
     8581 ccgaccacgg cgtggagcac ttccgttggt agcggcattg gcaacttcta ttccaatctt
     8641 catccggcac tggcggacaa cgttgtctat gcagcggacc gcgctggttt ggtaaaagcg
     8701 ctgaatgcgg atgatggcaa agaaatctgg tctgtcagcc tggccgagaa agatggctgg
     8761 ttctctaaag agcctgcatt actttctggc ggtgtgaccg tgtctggtgg gcatgtctac
     8821 attggcagcg aaaaggcgca ggtttacgcg ctgaatacca gcgatggtac tgtggcatgg
     8881 caaactacag tcgcgggtga agcactttcg cgcccggtgg tcagcgacgg tctggtgtta
     8941 atccacacca gtaacggtca gttacaagcg ctgaacgaag ctgacggcgc tgtcaaatgg
     9001 acagttaacc tcgatatgcc ttcgctctct ttgcgtggcg agtctgcgcc ggcaacggct
     9061 tttggtgcgg ccgtcgtggg tggcgataat ggtcgcgtca gcgcagtgct gatggaacag
     9121 ggccagatga tttggcagca gcgtatttcc caggcgaccg gttctaccga aattgaccgt
     9181 ctgagcgatg ttgacacgac tcccgtcgtt gttaacggcg ttgttttcgc gctggcctat
     9241 aatggtaacc tgacggcgct tgatctgcgc agtggtcaga ttatgtggaa acgcgaactg
     9301 ggttcggtga atgatttcat cgtcgatggc aatcgcatct atctggtcga tcaaaatgac
     9361 cgggtgatgg cgttgaccat tgatggcggc gttacgctgt gggcacaaag cgatctgctg
     9421 catcgcctgc tgacttctcc ggtgctgtat aatggcaacc tggtggtcgg tgacagtgaa
     9481 ggttatctgc actggattaa cgtcgaagat ggtcgtttcg ttgcccagca aaaagttgat
     9541 agttccggtt tccagactga accggttgcc gctgacggca aactgctgat ccaggcaaaa
     9601 gacggaaccg tgtactctat tacacgttaa tcgtctctgt cgttcacttt gaaaacggct
     9661 cctggacagg ggccgttttc ctgtttttaa caacgacgcg aatatagcgt gcgttgtctg
     9721 atgatttata aaaatgaggc tttaaacatg gtacctgtgg tcgcgcttgt cgggcgccct
     9781 aacgtaggaa aatccacgtt atttaaccgt ctaactcgca cccgagatgc gctggttgcg
     9841 gatttcccgg gtctgactcg tgaccgtaag tacggtcgtg cggaaattga aggccgtgag
     9901 tttatctgta ttgataccgg cgggattgat ggcacagaag acggtgtaga aacccgcatg
     9961 gcggaacagt cgctgctggc gattgaagaa gcggacgtcg tactgtttat ggtggatgcg
    10021 cgcgcgggcc tgatgccggc agatgaagcg attgccaaac atctgcgctc ccgtgaaaaa
    10081 ccgaccttcc tggtggcaaa caaaactgac ggtctggatc ccgatcaggc agtggttgat
    10141 ttctactcgc ttggtttagg tgaaatctac ccgatcgccg cgtctcacgg tcgtggcgta
    10201 ttaagtctgc tggagcatgt gctgctgccg tggatggaag atctcgcacc gcaagaggaa
    10261 gtcgacgaag acgctgaata ctgggcgcaa tttgaagcgg aagagaacgg cgaagaagaa
    10321 gaggaagacg acttcgaccc gcaaagtctg ccgatcaaac tggcgattgt gggtcgtccg
    10381 aacgtaggta agtctacact cactaaccgt attcttggag aagagcgcgt tgttgtttac
    10441 gacatgcctg gcacgacgcg tgacagcatc tacatcccaa tggaacgcga tggacgtgag
    10501 tatgtgctca ttgacaccgc tggcgtacgt aaacgcggca aaatcaccga tgctgtagag
    10561 aaattctccg taatcaaaac gttgcaggcc attgaagacg ccaacgtggt gatgttagtg
    10621 attgatgcgc gcgaaggtat ttccgatcag gatctctcgc tgctgggctt tattctcaat
    10681 agtgggcgct cacttgtcat tgtggtgaat aagtgggatg gcctgagcca ggaagtgaaa
    10741 gagcaggtga aagaaacgct ggacttccgt ctgggcttta tcgattttgc tcgtgtgcac
    10801 tttatctctg ccttgcacgg cagtggtgtt ggtaacttgt ttgaatcagt acgtgaagcg
    10861 tatgacagct ccacccgtcg tgtggggacc tctatgctga cgcgcatcat gacgatggct
    10921 gttgaagatc accaaccgcc gctggtacgc ggtcgtcgtg tgaagctgaa atatgcccac
    10981 gctggtggtt ataacccgcc gattgtggtg attcacggta atcaggtgaa agacctgcct
    11041 gattcctaca agcgctactt gatgaactac ttccgcaaat cgctggacgt aatgggatcg
    11101 ccgattcgta ttcagttcaa agaaggggaa aacccgtatg cgaacaagcg taacaccctg
    11161 acgccaaccc agatgcgtaa acgtaagcgt ctgatgaagc acatcaagaa aaataaataa
    11221 ttatccgtaa gtattttatc tgccattccc tctatgaatg tagagggaat ggctaccgga
    11281 ggaattcata tggaactgca ttgtccaaaa tgtcagcatg tgcttgatca ggataacggc
    11341 catgcgcgtt gcccatcctg cggggaattt attgaaatga aagctctttg tccagactgc
    11401 catcaaccgt tgcaggtgtt aaaagcctgc ggtgcagtag attacttctg tcagcacggg
    11461 catggcttga tatcgaaaaa acgcgttgaa ttcgtgctgg cttaatgaac ctttttacgc
    11521 gattttttta ccggctggat gttttttact tcactttcta tccagccgtc ttccagacgt
    11581 gtggttagca tttcacccgc tttaacttgc ttcacttttt tcagtacatt gccgtcagta
    11641 gcagtagtaa cgctgtatcc acgcgccagc gttgacagtg ggcttacggc ttcgaggtgc
    11701 gttactgcat taccgaaacg ttcacgcgtg gcgctaagct gtgcgcgcag gatttctgct
    11761 aaacgatatt ccagttgctg aatgcgcgtt tgcgtgcgat gaatcttcgg ctgtggattt
    11821 tgctgattca gccgctgtgt taatcgctgc tgctgttgcc cggcacgctt aagctgattt
    11881 tccagcgcaa agctcatccg tttttgcagg cgttcaagca tggtttgctg gcgtgccagc
    11941 cggagctgcg gatgctgctg ctgcaagcga tgatgaatct gcgtaaagcg acgcgtacgg
    12001 ttggcgagat aataatccat cgccatctcc agccgttgac gggccgattg cacctggcgc
    12061 agtaactctt gctgattacg gctcactact tcagcggcgg cagacggcgt tggcgcacgc
    12121 agatcggcaa caaaatcggc aatggtcaca tccgtctcat gcccgacggc gctgacgacc
    12181 ggaatgcggc tggcaaaaat cgcccgcgct acgcgttcgt cgttaaaact ccataaatct
    12241 tccagcgaac cgccgccgcg cccaacgatc aacacgtcgc actcattgcg ctgattcgcc
    12301 agttcaatgg cgcgaacaat ttgccccggc gcgtcatcgc cctgaacgga ggtggggtag
    12361 atgatcaccg gtagagaagg atcgcgacgt tttaacacat gcaaaatatc atgtagcgca
    12421 gcaccggttt ttgaggtgat cacaccaacg caatgcgcag gggagggaag tggttttttg
    12481 tattgctgat cgaacaaacc ttcagcctgc aacttcgctt tgagctgttc gtacttctgt
    12541 tgcagcagcc cttcaccggc cggctgcata ctctcaacga ttatctggta gtcgccgcgc
    12601 ggctcgtaga gcgtaatatt ggcgcgaact aaaacttgtt gcccatgctg cggacggaag
    12661 gtgacccggc ggttgctgtt gcggaacatc gcgcagcgta cctgggcggt gtcgtctttg
    12721 agtgtaaagt accagtgacc ggaagccggt tgtgtgaaat tagagatttc gccgctgatc
    12781 caaacctgtc ccatctcatg ctcaagcagc agacgaaccg tttgattcag gcgactaacg
    12841 gtaaaaattg caggggattg agaaggtaac atgtgagcga gatcaaattc taaatcagca
    12901 ggttattcag tcgatagtaa cccgcccttc ggggatagca agcatttttt gcaaaaaggg
    12961 gtagatgcaa tcggttacgc tctgtataat gccgcggcaa tatttattaa ccactctggt
    13021 cgagatattg cccatgctac gtatcgctaa agaagctctg acgtttgacg acgttctcct
    13081 cgttcctgct cattctaccg ttctgccgaa tactgctgac ctcagcaccc agctgacgaa
    13141 aactattcgt ctgaatatcc ctatgctttc cgcagcaatg gataccgtaa cggaagcgcg
    13201 cctggctatt gctctggctc aggaaggcgg tatcggcttt atccacaaaa acatgtccat
    13261 tgaacgccag gcagaagaag ttcgccgtgt gaaaaaacac gaatctggtg tggtgactga
    13321 tccgcagact gttctgccaa ccacgacgct gcgcgaagtg aaagaactga ccgagcgtaa
    13381 cggttttgcg ggctatccgg tcgttaccga agaaaacgaa ctggtgggta ttatcaccgg
    13441 tcgtgacgtg cgttttgtta ccgacctgaa ccagccggtt agcgtttaca tgacgccgaa
    13501 agagcgtctg gtcaccgtgc gtgaaggtga agcccgtgaa gtggtgctgg caaaaatgca
    13561 cgaaaaacgc gttgaaaaag cgctggtggt tgatgacgaa ttccacctga tcggcatgat
    13621 caccgtgaaa gacttccaga aagcggaacg taaaccgaac gcctgtaaag acgagcaagg
    13681 ccgtctgcgt gttggtgctg cagttggcgc aggtgcgggt aacgaagagc gtgttgacgc
    13741 gctggttgcc gctggcgttg acgttctgct gatcgactcc tcccacggtc actcagaagg
    13801 cgttttgcag cgtatccgtg aaacccgtgc taaatatccg gatctgcaaa tcatcggcgg
    13861 caacgtggca acagctgcag gtgcacgcgc tttggcagaa gctggttgca gcgcggttaa
    13921 agtcggtatc ggccctggtt ctatctgtac gactcgtatc gttactggcg taggtgttcc
    13981 gcagatcact gccgttgctg acgcagttga agccctggaa ggcaccggta ttccggttat
    14041 cgcagatggt ggtattcgct tctccggcga catcgccaaa gctatcgccg ctggcgcaag
    14101 cgcggtgatg gtaggttcca tgctggcggg tactgaagaa tctccgggtg aaatcgaact
    14161 ctaccagggc cgttcttata aatcttaccg tggtatgggt tccctgggcg cgatgtccaa
    14221 aggttcctct gaccgttatt tccagagcga taacgctgcc gacaaactgg tgccggaagg
    14281 tatcgaaggt cgcgtagctt ataaaggtcg cctgaaagag atcattcacc agcagatggg
    14341 cggcctgcgc tcctgtatgg gtctgaccgg ctgtggtact atcgacgaac tgcgtactaa
    14401 agcggagttt gtacgtatca gcggtgcggg cattcaggaa agccacgttc acgacgtgac
    14461 cattactaaa gagtccccga actaccgtct gggctcctga ttctcttcgc ccgacttcat
    14521 gtcgggcgat ttatattatc tgtttcactt gcctcggaat aagcgtcaat gacggaaaac
    14581 attcataagc atcgcatcct cattctggac ttcggttctc agtacactca actggttgcg
    14641 cgccgcgtgc gtgagctggg tgtttactgc gaactgtggg cgtgggatgt gacagaagca
    14701 caaattcgtg acttcaatcc aagcggcatt attctttccg gcggcccgga aagtactact
    14761 gaagaaaaca gtccgcgtgc gccgcagtat gtctttgaag caggcgtacc gatattcggc
    14821 gtttgctatg gcatgcagac catggcaatg cagttgggcg gtcacgttga agcctctaac
    14881 gaacgtgaat ttggctacgc gcaggtagaa gtcgtaaacg acagcgcact ggttcgcggt
    14941 atcgaagatg cgctgaccgc agacggtaaa ccgctgctcg atgtctggat gagccacggc
    15001 gataaagtta ccgctatccc gtccgacttc gtcaccgtag ccagcaccga aagctgcccg
    15061 tttgccatta tggctaacga agaaaaacgc ttctatggcg tacagttcca cccggaagtg
    15121 actcacaccc gccagggtat gcgcatgctg gagcgttttg tgcgtgatat ctgccagtgt
    15181 gaagccctgt ggacgccagc gaaaattatc gacgatgctg tagctcgcat ccgcgagcag
    15241 gtaggcgacg ataaagtcat cctcggcctc tctggtggtg tggattcctc cgtaaccgca
    15301 atgctgctgc accgtgctat cggtaaaaac ctgacttgcg tattcgtcga caacggcctg
    15361 ctgcgtctca acgaagcaga gcaggttctg gatatgtttg gcgatcactt tggtcttaac
    15421 attgttcacg taccggcaga agatcgcttc ctgtcagcgc tggctggcga aaacgatccg
    15481 gaagcaaaac gtaaaatcat cgggcgcgtt ttcgttgaag tattcgatga agaagcgcag
    15541 aaactggaag acgtgaagtg gctggcgcag ggcaccatct accctgacgt tatcgaatct
    15601 gcggcttctg caaccggtaa agcacacgtc atcaaatctc accacaacgt gggcggcctg
    15661 ccgaaagaga tgaagatggg cctggttgaa ccgctgaaag agctgttcaa agacgaagtg
    15721 cgtaagattg gtctggagct gggcctgccg tacgacatgc tgtaccgtca tccgttcccg
    15781 ggaccaggcc ttggcgttcg tgttctgggt gaagtgaaga aagagtactg tgacctgctg
    15841 cgccgtgctg acgccatctt cattgaagaa ctgcgtaaaa cggacctgta cgacaaagtc
    15901 agccaggcgt tcaccgtctt cctgccagta cgttccgttg gcgtaatggg cgatggtcgt
    15961 aagtatgact gggttgtctc tctgcgtgct gtcgaaacca tcgactttat gaccgcacac
    16021 tgggcgcatc tgccgtacga tttcctcggt cgcgtttcca accgcattat caatgaagtg
    16081 aacggtattt cccgcgtggt gtatgacatc agcggcaagc cgccagctac cattgagtgg
    16141 gaatgatttt gatccctgca ctatgaatga acaaaaccct ccgttactac agagggtttt
    16201 ttatcttcaa gaatcatagg cttgaagtta ctaacatcga ttaattaaac cagctgtccg
    16261 atttgttctc ttctgctttg cccacgcttt tcatcagatc gcgaccgcct tcagtcatat
    16321 ttctgtttgc gtcagcttca gattgcacca catcggtttg cgcagctttg tgcttcagtt
    16381 cctgatcgat aaattcgttt tcgcgcttaa cgcgggcttc ttctttcgcc agcgccagtt
    16441 tttgtttctg aatctctaag ctgcgtagct catcttcata actttgatcg cgttttttgt
    16501 ccgcagtggc ttcggcgtcc agtttatcct gacgagcttt cttatttgcc gctgccgttg
    16561 ccgctctttt gttcgcggcg gcctgggcat ttgcgcgacg ttgcttctct tgctggattt
    16621 ccctgttgcg ctccgcgacc cattcgtcat gctgcctttg ctcttcattt ttaccttgct
    16681 gttccgcttc tgcgacagca gagagttgat cctgcaatga tgaggctata gccggatagc
    16741 ttaaggaggc taagatggcg caaagaaaaa ctttcttcat gactcctcct gattattagc
    16801 tcttttcagg acatttggta tttggctgaa tacgcgtttc gttatacgtt gtggtaataa
    16861 caacggctaa acctgtcgta aactggcact ctttaccctc ctgggtggag gtatacactt
    16921 tggtgccttc cttatatgtt aaagaaacac cttccactaa ggttttatca ttcaccatag
    16981 aacccgctgc cgcgcctaca gctccgccgc caactgcccc tgccgtcgtt ccggaattgc
    17041 tgccagaacc gacgttgtgg ccgataacac cgccagcgac tgcgccaata agcgcgccga
    17101 aggcttgtgc gttccgttta ttttgggagt tgtctacggc aacttttgcg ggaagaatgg
    17161 aaataatatt aacggtttta gtttcttgtt tggtattcag ttgatcggtt tgataaacat
    17221 cggcggcatg atcgtcagca tttgactggc atcctgccag agtgaatgac gctaacattg
    17281 ccacaggcag aagacatttt ttaaatttca tcactattcc ttgttataca cattacgaaa
    17341 aataagacat ttaagatcat caaaagagga aagtaaatgt attgcgtata aagggataga
    17401 ttaaatttat taaaattcac gcagggaata atttataaaa atatatcatt acaacatata
    17461 tattaatttt atatttattt cgcagccaaa caggataaac atcagtcagg acaaagacgt
    17521 aaaatttaag atgaaagaat atctgccgct ttgataatcc caaagagcaa aagcatccga
    17581 cagcgaagtg ccgttatgtt ctgtgaaagt ggagaaggta accgctaacg ttgtaccggt
    17641 gattcgacgg agagtgtgga ttgctgagct tcttttttgc tctgatggtg cagccaggca
    17701 ccgacggacg agtagataaa acggccaaag aagaagataa agctgatgag cagtacgata
    17761 cgggtcatgc gactgttaaa tcggtgtcgt tttcgcatac tagttgcctg actcacaaaa
    17821 ggttccttga agtatgtccc acgctgtgga cggtacttac attaaggcac aacaggacaa
    17881 aatggtcaat tcttcgttat gtaaaaaagc gtcagtggat acatatttta atgttatgga
    17941 agttaattta attatttaca atgatgatgt aataatgatg aattaacatg ataaacgtaa
    18001 taattcatta atcagggtta ttttgtttga tatatatcaa ttggaacttt catatgacac
    18061 ttagaatcaa tgctctctat agtgatgaat aatcatcatt cgaagtcagg tgggatgcct
    18121 gtctgaatac acctccttca ggatgtgggg gattctgctg agcatctatg aaactgaatg
//
LOCUS       DADLZM010000078        17944 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLZM010000078 DADLZM010000000
VERSION     DADLZM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.078"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..2264
                     /locus_tag="JNY73_002513"
                     /pseudo
     CDS             1..2264
                     /locus_tag="JNY73_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="JNY73_002514"
     CDS             2265..3914
                     /locus_tag="JNY73_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY8048491.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLAFLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="JNY73_002515"
     CDS             3919..4695
                     /locus_tag="JNY73_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY8048492.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="JNY73_002516"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="JNY73_002516"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY8048493.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="JNY73_002517"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="JNY73_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY8048494.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="JNY73_002518"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="JNY73_002518"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY8048495.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            12096..13217
                     /gene="ompC"
                     /locus_tag="JNY73_002519"
     CDS             12096..13217
                     /gene="ompC"
                     /locus_tag="JNY73_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY8048496.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13329..14384
                     /gene="apbE"
                     /locus_tag="JNY73_002520"
     CDS             13329..14384
                     /gene="apbE"
                     /locus_tag="JNY73_002520"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY8048497.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14458..15522
                     /gene="ada"
                     /locus_tag="JNY73_002521"
     CDS             14458..15522
                     /gene="ada"
                     /locus_tag="JNY73_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY8048498.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15522..16116
                     /gene="alkB"
                     /locus_tag="JNY73_002522"
                     /pseudo
     CDS             15522..16116
                     /gene="alkB"
                     /locus_tag="JNY73_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            16192..17835
                     /gene="yojI"
                     /locus_tag="JNY73_002523"
     CDS             16192..17835
                     /gene="yojI"
                     /locus_tag="JNY73_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY8048499.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPHAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         4337 a         4377 c         4823 g         4407 t
ORIGIN      
        1 ttaacctcgt cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat
       61 gaaatgatct acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg
      121 ctggggcgta acaatgtgcg taccagctcc agtctgtcgt ttatcagcta tgaccaggcg
      181 ctctccagcg agccggttgc gcctggcgcg actaaccgca gcgagcggcg agtaaaaatg
      241 cttgaacgtc cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc
      301 gataaacaag gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt
      361 atcaccgcgc gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt
      421 tcggaaaaaa atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa
      481 ccggcggcag gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact
      541 aaatttgcag gcgctgagat gcgccagacg ctgacgctgc acaaaggggc gaattatatt
      601 tcgctgacgc agaatattca gcaatctggc ttgttaagtg cagaactgca acaaaatggg
      661 caagtgcagg acagcattag cacaaaactg tcttttgtgg acaacagctg gcccgttgaa
      721 cagcagaaaa atgtcatgct cggcggtggc gagaacgcgc tgatgttgcc cgagcaggcg
      781 agtaatatcc ggctacaaag tagtgaaacg ccgcaggaga ttttccgcaa caatcttgat
      841 gcgttagtcg atgaaccgtg gggtggggtg atcaacaccg gtagccgtct gatcccgctc
      901 agtctcgcct ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag
      961 atgattcagg ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc
     1021 tggtggggtg aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgat
     1081 tggcaggcca gccaggcgct cggcgtaacg caacaaccgg aatactggca gcatatgctc
     1141 gacagttatg ccgagcaggc ggataacatg ccgttattgc atcgggcgct ggtgctggcg
     1201 tgggcacagg agatgaatct gccgtgcaaa acgttgttga aaggggggga tgaagctatc
     1261 gcccggcgcg gaactaaaga tgaagatttc tctgaggaag acatccgcga tatcaatgac
     1321 agcctgatcc tcgatacacc ggaatctcca ctggcagatg cggtggcaaa cgtcttaacc
     1381 atgacgttgc tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat
     1441 gcgtgggata aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgctcaat
     1501 agcggtggtg acgctaccca gacggccgct attttaatgg tttgaccgct gagcaatcca
     1561 ctattgagcg cgcgctggcc atgaactggc tggcgaaata tatggcgaca atgcctccag
     1621 ttgttttgcc tgcgcctgcg ggcgcatggg ctaaacataa gttaactgga gggggcgaag
     1681 actggcgttg ggttggtcag ggtgtgccgg acattctctc ttttggtgac gaattatcgc
     1741 cgcaaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttacctg cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagccga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtttcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtctcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccgg cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg tgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatca gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttcgcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gccctggcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaggccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattctt ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgcgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt aattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgcaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaggcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgtaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    11881 cgccattccg caataatctt aaaaagttcc cttgtattta cattttgaaa catctatagc
    11941 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    12001 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    12061 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    12121 tggtcccagc tctgctggta gcaggagcag caaacgctgc tgaagtttac aacaaagacg
    12181 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
    12241 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    12301 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    12361 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    12421 tcgactacgg tcgtaactac ggcgtagttt acgacgtaac ttcctggacc gacgtactgc
    12481 cagaattcgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaacggct
    12541 tcgcaaccta ccgtagcacc gacttctttg gtctggttga cggtctgaac ttcgctgttc
    12601 agtatcaggg caaaaacggt agcccggaag gtgaaggcat gaccaacaac ggtcgtgaag
    12661 ctctgcgtca gaacggtgac ggcgttggcg gttctattac ttatgattac gaaggcttcg
    12721 gtatcggtgc tgcagtttcc agctccaaac gtactgatga ccagaacttc ggtctgaacc
    12781 gttatgatga gcgttacatc ggtaatggcg accgcgctga aacctacacc ggtggtctga
    12841 aatacgacgc gaacaacatc tacctagctg ctcagtacac ccagacctac aacgcaactc
    12901 gcgtaggcaa cttgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
    12961 agttcgactt cggtctgcgt ccgtccctag cttacctgca gtctaaaggt aaaaacctgg
    13021 gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
    13081 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
    13141 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
    13201 tggtttacca gttctaatct cgatggatat cgaacaaagg gcctgcgggc ccttttttca
    13261 ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat
    13321 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggttgccgcg ctcttctttg
    13381 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
    13441 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
    13501 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
    13561 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
    13621 cggtaagtga agcgatggcc gatatcgtca ccacctcgct gcgcattggg gcaaggaccg
    13681 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
    13741 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
    13801 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
    13861 atttatatgt cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
    13921 tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc
    13981 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
    14041 aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct
    14101 accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg
    14161 ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag
    14221 ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc
    14281 gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga
    14341 tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg
    14401 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
    14461 aaaaacgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
    14521 aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg ccgtccgtct
    14581 tgccgcgcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca
    14641 ctcgccgccg gatttcgccc ctgcaaacgt tgtcagccag acaaagccaa tccccggcaa
    14701 catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg
    14761 ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa
    14821 gctactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
    14881 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
    14941 agcagcagtt actaccgcaa agccgatgaa acgctgggca tgacggctaa acaattccgt
    15001 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
    15061 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
    15121 ttaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg
    15181 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
    15241 ttaccgctgg atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
    15301 ataccttgtg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
    15361 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt
    15421 catcgagtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
    15481 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
    15541 atgctgaacc gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt
    15601 ttaatgctgc ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc
    15661 gccagatggt cgctccaggc ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
    15721 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
    15781 aaccgtggcc cgccatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
    15841 cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
    15901 aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccaatt gtttctgttt
    15961 ctctgggctt acccgcgatt tttcaatttg gtggcctggg gcggtgaatc gcggctgttt
    16021 tatcacggta ttcaaccgtt gaaagcgggg tttcatccac tcaccaccga ctgccgctac
    16081 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
    16141 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
    16201 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
    16261 gccagtgcgg cattaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
    16321 gcggatacca gtctgctggt gttaccggag tttctgggat tattgctgct gttgatggca
    16381 gtcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
    16441 cgtagtgaat ttatcaagcg gattctggat acccacgtcg agcgcattga acaactcggt
    16501 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
    16561 cgtctgccgg aactggggca ggggatcatt ctcactatcg gttcatcggc gtatctgtgg
    16621 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcgatcac catctggggc
    16681 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctacgtga aaccgaagac
    16741 aagctgtaca cggattttca aactgtactt gaagggcgca aagagctgac tctgaaccgg
    16801 gaacgcgccg agtatgtgtt taacaacctc tacatccccg atgcgcaaga gtatcgccat
    16861 catattattc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
    16921 ctgggcgcta tcggcctggt gttctggatg gcgaacagtc tcggttgggc tgataccaac
    16981 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
    17041 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
    17101 ctcgcgcctt tcaaagctga gtttccgcgc ccgcatgcgt ttcccaactg gcaaacgctg
    17161 gagctgcgta acgtgacgtt tgcttatcag gataacgcgt tttccgttgg tccgattaat
    17221 ctcaccatca aacgtggcga gctgctgttt ctgattggcg gcaacggtag cggaaaatcg
    17281 acgctggcga tgttgttgac gggcttgtat cagccacaaa gcggcgaaat cttgctggat
    17341 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
    17401 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
    17461 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
    17521 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtgccgct gttgctggcg
    17581 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
    17641 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
    17701 ttcgctatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
    17761 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
    17821 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgtca ggtcaatatg
    17881 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
    17941 aaat
//
LOCUS       DADLZM010000079        17649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLZM010000079 DADLZM010000000
VERSION     DADLZM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.079"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            144..473
                     /locus_tag="JNY73_002524"
     CDS             144..473
                     /locus_tag="JNY73_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY8048500.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            721..966
                     /gene="nrdH"
                     /locus_tag="JNY73_002525"
     CDS             721..966
                     /gene="nrdH"
                     /locus_tag="JNY73_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY8048501.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            963..1373
                     /gene="nrdI"
                     /locus_tag="JNY73_002526"
     CDS             963..1373
                     /gene="nrdI"
                     /locus_tag="JNY73_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY8048502.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1346..3490
                     /gene="nrdE"
                     /locus_tag="JNY73_002527"
     CDS             1346..3490
                     /gene="nrdE"
                     /locus_tag="JNY73_002527"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY8048503.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLQEAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3500..4459
                     /gene="nrdF"
                     /locus_tag="JNY73_002528"
     CDS             3500..4459
                     /gene="nrdF"
                     /locus_tag="JNY73_002528"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY8048504.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            4814..6016
                     /locus_tag="JNY73_002529"
     CDS             4814..6016
                     /locus_tag="JNY73_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY8048505.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6009..7073
                     /gene="proW"
                     /locus_tag="JNY73_002530"
     CDS             6009..7073
                     /gene="proW"
                     /locus_tag="JNY73_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY8048506.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7131..8123
                     /gene="proX"
                     /locus_tag="JNY73_002531"
     CDS             7131..8123
                     /gene="proX"
                     /locus_tag="JNY73_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY8048507.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8315..9494
                     /locus_tag="JNY73_002532"
                     /pseudo
     CDS             8315..9494
                     /locus_tag="JNY73_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9618..10355
                     /locus_tag="JNY73_002533"
     CDS             9618..10355
                     /locus_tag="JNY73_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY8048508.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10345..10680
                     /gene="ygaH"
                     /locus_tag="JNY73_002534"
     CDS             10345..10680
                     /gene="ygaH"
                     /locus_tag="JNY73_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY8048509.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            10771..11301
                     /gene="emrR"
                     /locus_tag="JNY73_002535"
     CDS             10771..11301
                     /gene="emrR"
                     /locus_tag="JNY73_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY8048510.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMN"
     gene            11428..12600
                     /gene="emrA"
                     /locus_tag="JNY73_002536"
                     /pseudo
     CDS             11428..12600
                     /gene="emrA"
                     /locus_tag="JNY73_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            12617..14155
                     /gene="emrB"
                     /locus_tag="JNY73_002537"
     CDS             12617..14155
                     /gene="emrB"
                     /locus_tag="JNY73_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY8048511.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="JNY73_002538"
     CDS             complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="JNY73_002538"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY8048512.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="JNY73_002539"
     CDS             complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="JNY73_002539"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY8048513.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(16513..16941)
                     /locus_tag="JNY73_002540"
     CDS             complement(16513..16941)
                     /locus_tag="JNY73_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8048514.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="JNY73_002541"
     CDS             complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="JNY73_002541"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY8048515.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVHPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4183 a         4592 c         4495 g         4379 t
ORIGIN      
        1 ctcgttattt tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
       61 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
      121 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
      181 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
      241 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcgt
      301 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
      361 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
      421 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa
      481 atgataataa atacgcgtct ttgaccccga agcctgtctt cggggtttct ttttgcctgg
      541 tgaatcacaa aaatccccct cccccgtcac gctcatatcc agggtaattt cgatcactat
      601 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
      661 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacaga aatacgaatc
      721 atgcgcataa ctatttatac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
      781 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg
      841 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
      901 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
      961 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
     1021 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     1081 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     1141 gacaggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
     1201 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     1261 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
     1321 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
     1381 aacgatggat taccacgcgc tgaatgcgat gcttaatctt tacgatagcg caggtcgcat
     1441 tcagttcgat aaagaccgcc aggccgttga cgcctttatt gcgacgcatg tgcgtccgaa
     1501 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
     1561 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
     1621 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
     1681 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat
     1741 ggtggcgctg acgctggcac aaggcgatga gacgctggcg ttgcaactga ccgatgaaat
     1801 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
     1861 cggcgaactg gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg
     1921 ggcggtaaat tccgcactgc agctgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
     1981 gaatctgcag gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
     2041 tccggtgatg aaaatgttgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     2101 gggggcaggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac
     2161 gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     2221 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     2281 tgacgttgag cgagtttatg gcaagccgtt tgccgatatc gccattagcg aacactatga
     2341 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     2401 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacagt
     2461 aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt
     2521 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     2581 tatttcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgatttttc
     2641 ccgcacggta gagactgccg tgcgcggttt aacggcggtg tcagatatga gtcatatccg
     2701 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
     2761 gaatttacat ggttatctgg cgagggaagg catcgcttat ggttcgccgg aagccctgga
     2821 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     2881 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
     2941 atatttcagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     3001 gtttgcccgc agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga
     3061 cgtgatgcgc tacggcatat acaaccagaa tctccaggcg gtgccgccaa ccgggtctat
     3121 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     3181 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     3241 ggcgctgtat caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga
     3301 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     3361 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta
     3421 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     3481 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     3541 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     3601 aaaggtgccg ctgtcgaacg atattcccgc ctggcagaca ttaactgtcg tagaacaaca
     3661 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     3721 cgcgccttcg ctgatgcccg atgcgctcac gcctcatgaa gaggcggtat tatcgaatat
     3781 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     3841 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     3901 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
     3961 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     4021 ccgcggaaag ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt
     4081 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
     4141 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt
     4201 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     4261 ctgttacaac accaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga
     4321 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     4381 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
     4441 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc agcagggaaa
     4501 taattcccgc caaatagctt tttatcacgc aaatattctg tagtgatcta cactgatact
     4561 ctgttgcatt attcgcctga aactacaata ttcaggcgtt ttttcgctat ctatgacaaa
     4621 aaatatcaac ttttctcgat ttgctctcag cccttatatc acggaaaatt ccggcgattt
     4681 gctcgcatca atattcatgc cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt
     4741 tagggtagaa aaaagtgact attcccattg ggtaatatat cgacataaac aaataaagga
     4801 atcattctat tgcatggcca ttaaattaga aattaaaaat ctttataaaa tatttggtga
     4861 gcatccacag cgagcgttca aatatatcga acaaggactt tcaaaagaac aaattctgga
     4921 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     4981 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     5041 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     5101 cgacgccgaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     5161 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     5221 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     5281 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     5341 ccgcgcgtta gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga
     5401 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     5461 caccattgtc tttatttccc acgatcttga tgaagccatg cgtattggcg accgaattgc
     5521 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     5581 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tgttcagtgc
     5641 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaactcctg gcttcggccc
     5701 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     5761 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     5821 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     5881 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     5941 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     6001 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     6061 cgcaatccgc agacgcctgg gggacaccga cgactgcacc ggctgacggc ggtggtgctg
     6121 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     6181 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     6241 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc
     6301 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     6361 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ctcgctgatt gccatcggcg
     6421 caatcggtgc ctgttcgcag gcaatggtga ctctggcgct ggtgttaact gccctgctgt
     6481 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     6541 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc
     6601 caatcgtcat gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg
     6661 cgctgccgcc gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga
     6721 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     6781 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta
     6841 tggtggtcat tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     6901 tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     6961 ttatcctcga tcgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     7021 gctggtacac cactggccct gttggtctgc tgacccgccc attcattaag taactctgca
     7081 cttgcccggt gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     7141 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc
     7201 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     7261 agacgctgct ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg
     7321 aagtggatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     7381 tgaactggac gccactccat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     7441 atcgtgaagg ggtatttgtt aacggcgcag cacagggtta cctgatcgat aagaaaaccg
     7501 ccgaccaata caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     7561 tcgataccaa cggcgacggt aaagcggatt taaccggttg taaccctggc tggggctgcg
     7621 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     7681 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     7741 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     7801 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaatgcc gataccaaac
     7861 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     7921 cctggaccga gaaaaatccg gcggcagcca aactgtttgc cattatgcag ttgccagtgg
     7981 ccgatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     8041 agggccacgt tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     8101 aggcgctggc agcgcagaag taatttttat tcgggcggat aaggcgtttt cgcctcatcc
     8161 gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc ctatcatgcc tacaaaccac
     8221 atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc tattattgat
     8281 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
     8341 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca gtaactatta
     8401 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     8461 ctttattgtt accgccgcgc agttgggcta tgccgcaggt ctactgtttc ttgttcccct
     8521 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     8581 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     8641 cggtttattc tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     8701 ggataaacgc ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct
     8761 ggcacgaacc gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt
     8821 tgcttcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
     8881 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagca tgttcatcag
     8941 cgataagatc ctgcgcaccc gcgcgttgct gggctgcatg acctttgcca acttcagcat
     9001 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtga
     9061 cattggtctg tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
     9121 ttttgccgac aagggcaaat cgcaccacaa ctttcggtct gcttctgctg ttactttcat
     9181 ggctggcaat ctggtttggt cacacttccg tactggcgtt gattatcggc attctggtgc
     9241 tggacctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacatc
     9301 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
     9361 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
     9421 ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt catcgtcagg
     9481 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
     9541 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
     9601 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
     9661 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg gtggcctttg
     9721 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
     9781 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gccgggagta
     9841 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt ttgtatggcc
     9901 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctatgggcgt
     9961 ttggcctgac ggatgaggtt tttgccgccg ccaccgcaaa actggtacgc aataatcgcc
    10021 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    10081 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    10141 ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tctttccagc
    10201 gcaaacaatc tctttgcgtt accgcagctt tagttggtgc ccttgcaggc gtaacgctat
    10261 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    10321 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    10381 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    10441 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    10501 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    10561 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    10621 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    10681 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    10741 atcgactgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    10801 atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat ccttctgact
    10861 cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    10921 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    10981 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    11041 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    11101 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    11161 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagatcag
    11221 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    11281 gttctcgaag cgatgaacta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    11341 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    11401 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    11461 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    11521 ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca tttcgaagaa
    11581 accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt gtctggcagc
    11641 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgatgt tctggtcact
    11701 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    11761 gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa tattgaggtg
    11821 caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    11881 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac cagtgcccag
    11941 gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat tctggggact
    12001 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctagctg
    12061 gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtttcccg tcgtgccgtg
    12121 caaccgggtg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    12181 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgcat cggtcagccg
    12241 gtcaccatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    12301 ctggatatgg gcacaggtag cgcgttctca ctgcttccgg cgcaaaatgc gaccggtaac
    12361 tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa acagctggag
    12421 caatacccgc tgcgcattgg tttgtccacg ctggtaagcg tcaataccac taaccgtgac
    12481 ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac cgcgcgtgaa
    12541 atcagcctag cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    12601 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    12661 gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca ttgctaacgt
    12721 ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccaaggaa cgtgggtaat
    12781 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    12841 cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg cgtcgtgggc
    12901 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    12961 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc cgccagccaa
    13021 acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    13081 gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    13141 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    13201 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    13261 cctgcagatt atgctcgacc gcggtaaaga gctggactgt ttttcatcac aggaaattat
    13321 cattcttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg agctgaccga
    13381 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgctt
    13441 gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc cgcagttgtt
    13501 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    13561 tattccggtg attctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcggcg
    13621 gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    13681 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    13741 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    13801 ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta tcggcacgtc
    13861 gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    13921 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    13981 gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    14041 ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc tggggctggt
    14101 gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggcggtgcgc actaagtaca
    14161 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    14221 agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg ctgttgatgc
    14281 gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    14341 ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc tgcactttca
    14401 gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct ggcgtaccaa
    14461 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    14521 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggatcc
    14581 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    14641 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    14701 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    14761 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    14821 atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc tttttctttt
    14881 ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg tttccatttc
    14941 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    15001 cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc ctgttccgcc
    15061 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    15121 gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt taatgctatc
    15181 cagcgtttgc gcgacgcgtt tcagatcgtg gaacagatct ttacccacct gcagtaacgg
    15241 gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac cttcaaggat
    15301 cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    15361 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    15421 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    15481 taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    15541 atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct taccgtcttt
    15601 ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt taaggcccgc
    15661 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    15721 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    15781 tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa tcgccggaga
    15841 tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga taacgcggaa
    15901 atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc acttcgcttg
    15961 ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt gcatcagcgc
    16021 gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg tgttagaagt
    16081 gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    16141 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    16201 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    16261 atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    16321 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    16381 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    16441 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    16501 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    16561 atagcgtaac gctttaccaa ggcacaaaaa aaagattact ggcccccagg agatgcgcat
    16621 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    16681 gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg atgttttacg
    16741 caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    16801 tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg ccaccagcac
    16861 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    16921 aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc tgctgcacgg
    16981 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg gtgcacgccc
    17041 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatca
    17101 gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa tgcctctgcg
    17161 atggcgcttt cactccccgt tcctacagcc attggacgac gaccatgcca acttttcacc
    17221 acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac tgcttctgtt
    17281 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    17341 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaatatc gtactgaaga
    17401 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    17461 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    17521 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    17581 gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg aaggaaattt
    17641 tttggagaa
//
LOCUS       DADLZM010000080        17458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLZM010000080 DADLZM010000000
VERSION     DADLZM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.080"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            564..1334
                     /locus_tag="JNY73_002542"
     CDS             564..1334
                     /locus_tag="JNY73_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY8048516.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JNY73_002543"
     CDS             1325..1798
                     /locus_tag="JNY73_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY8048517.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGTLSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JNY73_002544"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JNY73_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY8048518.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JNY73_002545"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JNY73_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY8048519.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JNY73_002546"
     CDS             3233..3727
                     /locus_tag="JNY73_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY8048520.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JNY73_002547"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JNY73_002547"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY8048521.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /locus_tag="JNY73_002548"
     CDS             complement(6410..7432)
                     /locus_tag="JNY73_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY8048522.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8484
                     /locus_tag="JNY73_002549"
                     /pseudo
     CDS             7571..8484
                     /locus_tag="JNY73_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8699..10060
                     /locus_tag="JNY73_002550"
     CDS             8699..10060
                     /locus_tag="JNY73_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048523.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNY73_002551"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNY73_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY8048524.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNY73_002552"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNY73_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY8048525.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="JNY73_002553"
     CDS             12610..13899
                     /locus_tag="JNY73_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY8048526.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="JNY73_002554"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="JNY73_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY8048527.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="JNY73_002555"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="JNY73_002555"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY8048528.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="JNY73_002556"
     CDS             16225..16605
                     /locus_tag="JNY73_002556"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY8048529.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17458
                     /gene="hpaH"
                     /locus_tag="JNY73_002557"
     CDS             16673..>17458
                     /gene="hpaH"
                     /locus_tag="JNY73_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY8048530.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4293 a         4626 c         4377 g         4162 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgttt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtacgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacgattt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg cttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccacaagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgaact gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccatt
//
LOCUS       DADLZM010000081        17383 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLZM010000081 DADLZM010000000
VERSION     DADLZM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.081"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(40..771)
                     /gene="rlmB"
                     /locus_tag="JNY73_002558"
     CDS             complement(40..771)
                     /gene="rlmB"
                     /locus_tag="JNY73_002558"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY8048531.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(951..3392)
                     /gene="rnr"
                     /locus_tag="JNY73_002559"
     CDS             complement(951..3392)
                     /gene="rnr"
                     /locus_tag="JNY73_002559"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY8048532.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3431..3856)
                     /gene="nsrR"
                     /locus_tag="JNY73_002560"
     CDS             complement(3431..3856)
                     /gene="nsrR"
                     /locus_tag="JNY73_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY8048533.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4061..5359)
                     /gene="purA"
                     /locus_tag="JNY73_002561"
     CDS             complement(4061..5359)
                     /gene="purA"
                     /locus_tag="JNY73_002561"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY8048534.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5463..5660)
                     /locus_tag="JNY73_002562"
     CDS             complement(5463..5660)
                     /locus_tag="JNY73_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY8048535.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5742..6746)
                     /gene="hflC"
                     /locus_tag="JNY73_002563"
     CDS             complement(5742..6746)
                     /gene="hflC"
                     /locus_tag="JNY73_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY8048536.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6749..8008)
                     /gene="hflK"
                     /locus_tag="JNY73_002564"
     CDS             complement(6749..8008)
                     /gene="hflK"
                     /locus_tag="JNY73_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY8048537.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8094..9374)
                     /gene="hflX"
                     /locus_tag="JNY73_002565"
     CDS             complement(8094..9374)
                     /gene="hflX"
                     /locus_tag="JNY73_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY8048538.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9450..9758)
                     /gene="hfq"
                     /locus_tag="JNY73_002566"
     CDS             complement(9450..9758)
                     /gene="hfq"
                     /locus_tag="JNY73_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY8048539.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9844..10794)
                     /gene="miaA"
                     /locus_tag="JNY73_002567"
     CDS             complement(9844..10794)
                     /gene="miaA"
                     /locus_tag="JNY73_002567"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY8048540.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQVVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10787..12634)
                     /gene="mutL"
                     /locus_tag="JNY73_002568"
     CDS             complement(10787..12634)
                     /gene="mutL"
                     /locus_tag="JNY73_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY8048541.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12644..13981)
                     /gene="amiB"
                     /locus_tag="JNY73_002569"
     CDS             complement(12644..13981)
                     /gene="amiB"
                     /locus_tag="JNY73_002569"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY8048542.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14000..14461)
                     /gene="tsaE"
                     /locus_tag="JNY73_002570"
     CDS             complement(14000..14461)
                     /gene="tsaE"
                     /locus_tag="JNY73_002570"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY8048543.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14433..15980)
                     /gene="nnr"
                     /locus_tag="JNY73_002571"
     CDS             complement(14433..15980)
                     /gene="nnr"
                     /locus_tag="JNY73_002571"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY8048544.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15979..17118
                     /gene="queG"
                     /locus_tag="JNY73_002572"
     CDS             15979..17118
                     /gene="queG"
                     /locus_tag="JNY73_002572"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY8048545.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17101..17154)
                     /locus_tag="JNY73_002573"
     CDS             complement(17101..17154)
                     /locus_tag="JNY73_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048546.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3938 a         4955 c         4371 g         4119 t
ORIGIN      
        1 accatccaaa tctggatggc ttttcataat tcagaggaac tagctgcgct gacgaaccgc
       61 ttcaaataag caaattccgg ttgcaaccga aacgttcagg gaagaaacac ttcctgccat
      121 tgggatgctg atcaactcat cgcaatgttc acgggtcagg cgacgcatac cttcaccttc
      181 cgcgcccatc accagcgcca ggcgtccggt cattttgctc tgatagagtg tatgatccgc
      241 ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc aacatacgca tggtgcgcgc
      301 aaggttagtc acccgaatca gcggaacgct ttctgccgcg ccgcaggcta cttttttcgc
      361 cgtagcgttg agctgtgcgg agcgatcttt cggcacaatc accgcatgaa cgcctgcggc
      421 gtccgcacta cgcaggcacg cgccgaggtt gtgcggatcg gttacgccgt cgaggatcag
      481 caggaacggt tgatcgagcg aagcgatcag atccggcaga tcgttttcct gatactgacg
      541 tcctggcttc acgcgggcga taatgccctg atgcacggca ccgtcgcttt tctcgtcgag
      601 atattggcgg tttgccaact ggataaccac gccctgagac tcaagggcgt gaatcagcgg
      661 taacagacgt ttatcttcac ggccttttaa aataaagact tcctgaaaac gttcaggggc
      721 gcgctccagc agggcctgca ctgcgtggat gccgtaaatc atttcgctca ttaatgtact
      781 cgttgtttac gttttgcctg atgcactacg tttatccggc ctacatgatc tctgcaatat
      841 attgaatttg cgtgattatg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
      901 aagcgcattt tgtcagcaat ctaaccctct tcttttaaag agggtattga tcactctgcc
      961 acttttttct tcgccgcacg cttcgctttg gtcgctgcgg ctattttctg cgttttcgcc
     1021 gatggctttt tcgcttttct cgcgtctttc ttcgccgctt tcggcttcgt ttttttctca
     1081 ccgcggaagg cgctgtctgg ctcaaagttt acctttttac cgacctgacg acgcttgccg
     1141 ccttttttgc ctgcatcgcc ttttttcgct ttctcgcgcg ccgttttacc gacgttgcgc
     1201 ggtgcgcgtt cactggagat cagactaaag tcgattttgc gctcgtccat attaaccgct
     1261 tcgacgcgaa cttccacgcg atcgcccagg cgataagtct ggccgctgga ttcccccatc
     1321 aggcgttgcc ctacctggtc aaagcgatag tagtcattgt ccagcgaaga gacatggacc
     1381 agaccatcaa tgaacaagtc gtccagacgg acgaagaagc caaagccagt gacgctggaa
     1441 attacgcctt taaagacgtt acctacctgg tcgagcatga agtcacactt cagccagtca
     1501 gcgacatcgc gcgttgcttc gtcggcacga cgttccgcca tcgaacagtg ctgacccagt
     1561 tgcaacatct cttccatcga ataatggtag ccgccggttt cagtggtgtt gccctgatgc
     1621 ccctgctctt tcgccagcag atatttaatg gcgcggtgca gcgtcaggtc aggataacga
     1681 cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca gaccgaagtg accacggttt
     1741 tctggatcgt aaatcgcctg tttcatcgag cgtagcagca tggtttgcag catttctgca
     1801 tcaggacggt cggcaaccga ctccagcagt tccgcgtagt cacgcggttc cggcttgtta
     1861 ccacccggca gctccagccc cagctccgcc agcactgaac ggaaagaggt aatcgcttcg
     1921 gtgctcggct tgtcgtgaat acggaacagt gccggttctt tcgctttctc aacgaaacgc
     1981 gccgccgaga tattcgccag aatcatgcac tcttcaatta atttgtgcgc gtcgttacgc
     2041 tgggtctgtt cgatacgttc aatacggcgt tcagcgttga aaatgaactt cgcttcttcg
     2101 ctctcaaatg agatcccacc gcgttcttca cgggctttat ccagcacttt atagaggtta
     2161 tgcaactctt cgagatgctt aaccagcggg gcgtactgct cgcgcagatc ctgatcgccc
     2221 tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg
     2281 tagaatttgt agcccgtcag gcggcctttc gacgaaaccg tcatctcgca caccatacac
     2341 aggcggtcta cctgcgggtt gagcgaacac aggccgttag agagcacttc cggcagcatc
     2401 gggataacct gcgaagggaa gtacaccgac gtgccacggt tacgcgcttc tctgtccagc
     2461 ggcgttggcg gacgcacata gtagctgacg tcggcaatcg cgacccataa acgccagccg
     2521 ccgccgcgtt ttttctcgca gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca
     2581 atggtgacca gcggtaaatc gcgcaaatcg acacggcccg cttttgcttc ttccggcact
     2641 tcttctttca gccccgcaac ctgttgctca acagcctgcg gccagatata cggaatttca
     2701 tgggtacgca gagcgatatc aaccgccatg ccggtgccca tattgtcgcc cagcacttcg
     2761 acgattttac ccaccgcttt ggtgcggcga gtcggacgct gagtcagttc gactacgacc
     2821 acaaagccca tccgcgcgcc catgatctga tcgggcggga ttaagatatc gaagctcaga
     2881 cggctatcgt caggaaccac aaagccgacg cccgcttcgg taaagtagcg accaacaatc
     2941 tggctggttt ttggcaccag tacgcggaca atacgcgctt cacgacgacc tttacggtca
     3001 gcacccagcg gctgcgccag cacctgatcg ccatgaatgc aggttttcat ctgctcgctg
     3061 gagagataca aatcatcttt acgcccttca acccgcagaa agccgtagcc atcacggtgg
     3121 ccaataacgg tacctttcac caggtcgagg cgttccggca gcgcatagca ctgacgacga
     3181 gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc
     3241 tgctcttcgc cttcaatgtg cagttctacc gccagctcat cacggctggc cggtttttca
     3301 cgtttggtta aatgttcgag gataaattcc cgactaggga tgggattcgc gtatttttca
     3361 gcttcgcgtt cctggaaagg atcttgtgac atctcggttc ctccgttgtc atctctgatg
     3421 aagattttcg tcactccaac agcaataatt tataaagcgg ttgattctct tcaaccaaat
     3481 cggcaagcgt gtagttatcc agttccgtaa gaaaactttg cacggcctta gaaagcgcct
     3541 gtttcaaccg acaggcaggt gtaatgtggc aaaactcact gctgcaattc accagcgata
     3601 agggctccag ctcgcgcacc acatcaccaa tacgtatcgc actcgccggt ttacccaggc
     3661 gaatgccgcc attttttcca cgaacagcag tcacgtagcc ggcacgacta agttgattga
     3721 ttattttgac catatgatta cgggagacgc cgtagacgtc agtcacttca gaaatactgg
     3781 tcatccgccc ttctggcaat gacgccatgt agatcagcgc acgtaatccg taatcagtga
     3841 aactcgttaa ctgcacatca acctcaaaag ggaaatcggg aaaaaataca tttatattga
     3901 tgataaacca gccacaagct gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg
     3961 aggatttaac actgtgtcgg atagcgtaat ggcttaaccg acacagcaat gtatcacact
     4021 gttagcccgg caggcaaaat atctgccagg cgtaccagaa ttacgcgtcg aacgggtcgc
     4081 gcagaatcat ggtttcagta cgatccggac cggtagagat gatatcgatc ggcacaccgg
     4141 tcagctcttc aatacgcttg atgtagttca gcgccgcctg cggcaggccg ctacgatctt
     4201 tcacgccgaa agtggattca gaccagcccg gcatggtttc gtaaatcggc tctacacctt
     4261 tccagtcgtc agctgccagc ggagtggtag tcacttcgcg accatccggc atacggtaag
     4321 ccacgcagag tttaacctct ttcaggccat ccagaacgtc cagtttagtc aggcagaagc
     4381 cagacaggga gttcagctgt accgcacgac gaacggcaac ggtgtccagc cagccggtac
     4441 gacgacgacg acccgtagtt gcaccgaatt cgttaccctg cttgcagagg aactcgccag
     4501 tttcatcaaa cagttcggcc gggaaaggac ctgcacctac acgagttgag taagctttga
     4561 ggatacccag aacgtaatca acataacgcg ggcccaggcc ggaaccggtc gccacgccac
     4621 cagcagtggt gttggaagaa gttacgtacg gataagtacc gtggtcgata tccagcagcg
     4681 taccctgcgc accttcgaac atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt
     4741 cagaaacgtc aaccaccata gaagtcagga tgtcggcaac agccatcgta tcatccagaa
     4801 ctttctggta atcaaccgct tcagctttgt agtagttaac caactggaag ttgtgatatt
     4861 ccatcacttc tttcagtttt tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca
     4921 gaccgcgacg tgccacttta tcttcataag caggcccgat accacgaccg gtggtgccga
     4981 tcgctttcgc gccacgcgct ttctcacgcg cgttatccag cgcaacgtga taatcaagga
     5041 tcagcggaca tgcttcagac agcagcagac gctcacgaac ggggatgcca cggtcttcca
     5101 gttctttcat ctctttcatc agcgcggccg gagacagcac aacaccgtta ccgatgatgc
     5161 tggttacatt ctcgcggaga atacctgatg gaataagatg gagaacggtt ttttcaccgt
     5221 tgattacgag agtatggcct gcgttgtgac cgccctggta gcgtacaaca tatttagccc
     5281 gttcagtcag aagatcgacg atcttacctt taccttcgtc accccattgg gtgcccagta
     5341 cgacgacgtt gttacccatt tttcaaaatc accgtttgct taaaaatgga ttctaccatc
     5401 gctttttcag agttacagca ctttttgcac tcaaaaatga cctcgaacag tctgtttttt
     5461 gttcagccaa tcgttttcct caacatgtag tagaccacaa cgcccgcaac cacaagtcca
     5521 ccgccaaaac gacgtaaaat attatcgggc aaattggtca tcgcagagat catcttcttc
     5581 catgccttcg ggtaaagcat cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc
     5641 agccagattg tcgaattcat tcattatcct tatagaaaaa gaaaaccacc gacatccctg
     5701 aggatgcggt ggctttattg acctgtaccg cagtcgttat attaacgcgt tgcggaagtc
     5761 ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca tgaccatcac gtcctgattg
     5821 ccagagaagc tgttctcata agcacgcagg ctacggatga atgcgtagaa gtccggatct
     5881 ttactgaatg catcagcaaa cagtttggct gcttcggcat cgccttcacc acgcataatg
     5941 cggccctgac gctcagcttc tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc
     6001 agtttttccg cttcttcctg accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg
     6061 gcgcgcatac ggttgtagat cgcttcagac acttcggtcg gcaggttgat ctgcttgata
     6121 cgcacatcga caacttcaat acccagcgcc gccatactgt tcgggttgat gaccggaact
     6181 ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg caatggcgtt atctgctgcc
     6241 ggggtagtaa cttcatcttc tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg
     6301 agggtcagac gaccacggga atcggtgacg atatctttca cgtccaggcg accaatttca
     6361 gaacgcagac ggtcagagaa cttacgtttc aacagcactt ccgcttgcga aatgtcgcca
     6421 ccacccgttg ccaggtagta acggctgaaa tcgctgatgc gccatttgat gtaagagtcg
     6481 acgatcaggt ctttcttctc tttggtcaca aagcggtcgg cctggttgtc catggtctga
     6541 atacgtgcgt cgagcatttt caccgtttca atgaacggta tcttgaaatg cagaccaggc
     6601 tcataaacca aaggtttgtt gtcatcgtca cgcagtacct taccaaaacg cagcgtaata
     6661 ccgcgctcac cttctttgac gacaaagaca gacatgtaaa gcactaccag cacgatgatg
     6721 ataatcgcga taactgactt acgcatcgtt attccccctg acgctggtag tcgttacgct
     6781 gcgcgttggc gcggcgttgg tccataatat cgccctgact ggtggacgac gtgttgcttg
     6841 ctccactggt tgtggaggaa gaggctggcg gcagacgcag cagattgctg gcaccgttat
     6901 cgctcttcgc cgcaggggcg ttaccacctt tcaacatctg gtctaacggc agaaccatca
     6961 ggttgccacc tttatcgtta accagcactt tgcgggtgtt acccaacact ttttccatcg
     7021 tctcgatata cagacgctcg cgagtaattt ccggcgcggc tttatattcc ggcagaagtt
     7081 tagcaaagcg cgccacttca ccctgagctt ccaggatggt ctgggccttg tacgcacgcg
     7141 cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg
     7201 cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg
     7261 ccgcttttac ttcttccggc ggacgagcag cctggaagtt gacgtccagc agcgtgatac
     7321 ccatgtcata cggacgaatc gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg
     7381 tacgaccttc cgtcagaatg cggtccatgg tgtatttacc gataactcca cgcagggcgc
     7441 tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac gctatacaga tatttttccg
     7501 gattggtgac gcggtactgc acgttcatct caacgcgcac tacgttctcg tccgacgtca
     7561 gcatcacacc agaagcggcc agttcacgca cggcttccac gttcaccggt ttgacttcgt
     7621 cgataaacgt cggtttccag ttcagacccg gctcaaccag atggctgaat ttaccaaagc
     7681 gtgttaccac gccgcgttcg gcttctttaa tggtatagaa accactggcc gcccagataa
     7741 tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc aagctgcggg cgcgggcctt
     7801 gcgatgaact gccaccgcca gatccggtgc ctttaccgcc gcccagacca ccgagctttt
     7861 tgctcagttt gcggaagata tcatctaaat caggtggccc ttgatcgcga ccgcctttgt
     7921 ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc
     7981 cgttattacc gggctgattc cacgccatgt ttgtgctcca tatttgttat gcggtgatcc
     8041 ctgttgtctt tcgacgccag aggatatgac tccacgcttc agacgctacg ccgttagatc
     8101 aggtaatcga tcaacgccgg ttcttgttta cagaggcgac gccagtcaac gatcggcata
     8161 cgaacttgca gacttacgct gccgtcctcc tccatccact ctttttctat tgcctgaagc
     8221 tgataaaaac gactcctcag acgcccttcc tgcggtggca gacgcaatgt atgctgcgcc
     8281 acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc
     8341 tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt cccgatcaat acgcggttcg
     8401 aaatcttcca gcatatcgat cttgttcatc accagcaggg ttgggatctc gtgagcgtcg
     8461 atctcttcaa gaaccgtatt caccgcttcg atgttttctt gtacacgcac atccgccgca
     8521 tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgct
     8581 gccaccagat cgtgcggcag gtggcgaata aaccctacgg tatctgcaag tacggtttca
     8641 ccgacatctg caacgtcaat acgccgcaac gtcgggtcga gggtggcaaa caactggtct
     8701 gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta
     8761 tatcccacca gcgaaacagt aggaacgtcg gctttgatac gcgattgccg cccctgctca
     8821 cgctgctttt caactctttc caggcgcgac tgtatctgca cgatgcgatt acgcaacaaa
     8881 cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca aacctatccc gcctttctgt
     8941 ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc
     9001 tcaacctgca acttaccctc atgggtacgc gcacgttggg cgaaaatatc taaaataagg
     9061 ccggtgcggt cgataacacg acactcgcac aaacgctcca ggttacgctc ttgcgccggg
     9121 ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg ctttgacagc ttccgcaatt
     9181 tcaactgctt taccttcacc tacaaaatac tttgggtgcg gcgctttacg gctaccggta
     9241 atcacctgca atgcttcgac accggcggaa gagaccagag attcaaactc ctggaggtct
     9301 tccatatctt tgtcttgcgt aaaatagatg tgtaccagta ccgcctgctc accagcatca
     9361 taacggtcaa acaagcgtat aaccctctaa atagatcagc ggggaacgca ggatcgctgg
     9421 ctccccgtgt aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg
     9481 gaagtattct gtgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg
     9541 ttactgtgat gagaaaccgg gcgagatggg acaacagtag aaatcgcgtg cttgtaaacc
     9601 atctggctga ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct
     9661 tgcagcttaa taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg
     9721 ttcaggaacg gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt
     9781 atttgtactt tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca
     9841 cattcagcct gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt
     9901 ttcactgtca agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc
     9961 caactgtctc gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgccttc
    10021 aaggtaagac cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa
    10081 atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg
    10141 aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg
    10201 atgcacctga tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc
    10261 cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct
    10321 tgctgccgca accggatcta ccacctgaag ttgacgatgc aatgactccc aaccttgctc
    10381 tgccgcctgt tgctcaattc tggcccgcac ttccgggtct gccgacggta gcggcgacaa
    10441 cccttccagc aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc
    10501 cgccgcggtg atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta
    10561 agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc
    10621 gttcggcttc gccgtcccga tatccatccc tttgtaaata agggcagaat caacgcttat
    10681 caactctact ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt
    10741 cggccccatc aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca
    10801 gggcttttat cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca
    10861 caagttgcgg acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc
    10921 actgcgcatg ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga
    10981 atacggactg cttcgccagg tagcctatca gttcaggaat caagatttgt aaattttgtt
    11041 ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa
    11101 tacccaattc cgctagggca gactgcgctt tttctaatgc cgatttttct tcgccagaaa
    11161 cttttagccg caacggaatc agcagcggct gggcgcaaac gggcgcttca cccggcgtca
    11221 attgtgcctg acgcagccaa cgttctgcca ctggcaagga taaaagtgaa atgttgccat
    11281 cgcgctccag caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac
    11341 tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg
    11401 gcgtttgcaa aagctggcga tacacttcac cttgctgttt ctggtagcct ggctgcgcat
    11461 tcggccaggg ggcagccgga cgactgcctg aggcgggcgc aggagagtag cgcggagcta
    11521 ccggctcacg agctgccggt tctgcaaagt gattgcgccc cgccgccacg cggttttccg
    11581 ggatggcacg cggtgcgggt tgtggttcat cgtccagcgg tagcggcgtt tccagttgct
    11641 gttgtagcac gctcagcacg ccctgataga taaaatcatg caccagacgc gactgatgga
    11701 aacgcacttc gtgtttggcg gggtgtacgt tgacgtccac ctgatgcggg tcgatctcca
    11761 gatacaacac aaatgccggt tgctgatcgg cccccagttt gtcttcgcag gcctggcgga
    11821 tcgcatgatt gatcaggcga tcgcgcatca tgcgaccatt cacgtagcaa tactgaattt
    11881 ccgccagtgc gggcgtagtg tgatttggat cggccaccca gccgcgcagg gtgagatcgc
    11941 cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc ggtgccgcaa atcgcgccta
    12001 agcgccgttc tttttgcccg ccttccggca ctgcgcggta ctgacgcaca attttaccgt
    12061 tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc aatgcggcgg atgatttcat
    12121 caatatggct aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt
    12181 agaacagatc cagcacctcc agcgtcgttc ccaccggatg cgccgccggt ttaaccgtca
    12241 cgtccatgtc gcgtccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg
    12301 aagtgagcgt caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca
    12361 ggctgataat ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgagcca
    12421 gcgccagcgc cagctcatct tttttgatac cgcagccgtt atcacgaatg cggataagtt
    12481 tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt
    12541 ccactagttc tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt
    12601 tcgccagttg tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc
    12661 gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc accctgcggc gcagattgca
    12721 tcggatgcgc aaggaagtaa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt
    12781 ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga
    12841 ccagtactga tgggatatcc ggcgagcgca gaacgccaag gctggcgtgt tctggtcgac
    12901 gtttatgtat ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc
    12961 ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag
    13021 actgactgtt cgccagcaca tcacccgccc cacccagcag ctccgactgt ttctcgtgct
    13081 gctccagcca gctggccatt tcactgttgg cgcgacggtt agagagcacc aatacggaag
    13141 cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt
    13201 tttgcttatg tgccacatca ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg
    13261 ttaaaacgcc tttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg
    13321 cgatggtgac atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc
    13381 cgtgtccggc atcaatagcg atgataattt tatcgccagt gttagccgtc gcgcgcgctg
    13441 ccggacgcgt taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa
    13501 acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt
    13561 tcgcaaccac gggcggcgga ggaggcggtg gcggcacatc ggcgttaatc gtaaagacga
    13621 cagtgtaatt gctgccattc tgccgcttca ccgcttcggt tttaccgttt tcggtaagat
    13681 cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc agagcgaatc gccttcacca
    13741 gattattgcc gctgaacaac aacggcagtc cctgaatcac gcccgtttgt ttgatatcga
    13801 gcgccacggt gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca
    13861 acgttatccg tgcctgttga ttaccgttag aaacctgaat atcagagagc gtcgcggcac
    13921 ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca atttctgatg cgatacatca
    13981 tcccgccacc tttcaaaggt taaccggcta aacgcgccag caacaattca cccgcagagg
    14041 aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat
    14101 ccgggtcagg aagaacacct gtaccttgtt gtggccactc cacaaggcag atggcatcgt
    14161 tggcaaaata atcgcggatc cccataaact ccagctcctc gggatcggca aggcggtaca
    14221 aatcaaagtg atagaccatt aagttgtcga gcgtataggg ttcgaccagc gtataagtgg
    14281 ggcttttgac attaccctga tgacccagag cctgtaaaaa gccccggcta aaggtggttt
    14341 tacctgcgcc taaatcgcca tacagataga ttacggttgc tccatcgcag gctttcgcta
    14401 cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact cgattcatca
    14461 tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt
    14521 gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg
    14581 acacagcctg cacaggctgc atcatacggc gacagttttt gcccaagcaa tgcgccaata
    14641 ataccagaga gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc ggcatcaata
    14701 atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg
    14761 ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg
    14821 acggaacagc ctaacaaccg tgcggcctcg ctaggatgcg gcgtgatcac gcgattgtga
    14881 cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg
    14941 cgaaaattct caactttttg cagggctttt ttcccccact cttgctggcc cagaccggga
    15001 ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat agtcagttca
    15061 tgcaccatca attctggtcg tgcagtcagc agcggcgcaa tgttctcact gcgggtcagt
    15121 actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc
    15181 gccgtgccgt gatcgccacc gataatcacc agccgcccgt gatcgccttt atgcgaagtc
    15241 gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc
    15301 tcctgacctg ccagccagct atccagcccc agtgagtcaa aatgcagttg tcctgtaaca
    15361 tcccgcgctt ttccagtgag caagcctagt ttcagcgcaa taaaagtgat ggtgtgatcg
    15421 gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca
    15481 accgccacaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg
    15541 cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc taccgattcg
    15601 ggccagacaa tattcgaagc atggatctcg ccacccgcgt ttaaccatgc ttcgcgtgcc
    15661 agcgcggcct cttccggcaa cggtttgtcg ctctcctggg ccaacaacgt gacctcaatg
    15721 ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc cgccgttatt accatgaccg
    15781 cacagcacca gccagtggcg ggcgtcagga tacgccgaac gacacacctg gaatgcggcc
    15841 tcgccagcgc gaagcatcag ctcatagagt gtgagcccca gcacatctgc cgcctcgcgt
    15901 tctccgcggc ggatatcgtc ggcgtaccag acggtgtgtg gtatacttac ggggtttttc
    15961 ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt
    16021 aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc
    16081 gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg
    16141 atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattacccgg tacgctgcgt
    16201 gtaatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa
    16261 aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg
    16321 cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt
    16381 agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc
    16441 ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcgtt cttcttttta
    16501 ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggc
    16561 aaatgcgtgg cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc
    16621 gatgctcgcc gctgtatctc ttatctcacc atcgaacttg aaggggcgat cccggaagag
    16681 ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg
    16741 tggaatcgct attcgcaact cactacagaa gacgatttca gcccgcgtaa gccgctacac
    16801 gcaccggaac tcattgagtt atttgcctgg agcgaagaga agtttttaaa agtcacggaa
    16861 ggatcggcga ttcgccgtat aggtcacctg cgctggctgc gtaatatcgc cgtggcatta
    16921 ggcaatgccc cttgggatga aacgattttg acagcgctgg aaagtcgtaa aggtgagcac
    16981 ccacttctcg atgagcacat agcgtgggcg attgcgcagc aaatagagag acgaaatgcg
    17041 tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg
    17101 ttaccgcgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat
    17161 tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cggaatcttg atttttaact
    17221 tatttatcaa taggttaaaa aataccattc accgtgcgaa gtttctttga aattaagatt
    17281 acagcctgag gctgtggata actctgttta caagagtttg tcagagacaa caaaaaccag
    17341 caataacgca tcgcgtcgct gtggatattt tattgagaga aga
//
LOCUS       DADLZM010000082        17372 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.082, whole genome shotgun sequence.
ACCESSION   DADLZM010000082 DADLZM010000000
VERSION     DADLZM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.082"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..600)
                     /locus_tag="JNY73_002574"
     CDS             complement(<1..600)
                     /locus_tag="JNY73_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8048547.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHKVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
     gene            complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNY73_002575"
     CDS             complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNY73_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY8048548.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNY73_002576"
     CDS             complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNY73_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY8048549.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNY73_002577"
     CDS             complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNY73_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY8048550.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2535..4193
                     /gene="fliF"
                     /locus_tag="JNY73_002578"
                     /pseudo
     CDS             2535..4193
                     /gene="fliF"
                     /locus_tag="JNY73_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            4186..5181
                     /gene="fliG"
                     /locus_tag="JNY73_002579"
     CDS             4186..5181
                     /gene="fliG"
                     /locus_tag="JNY73_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY8048551.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVNDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5174..5860
                     /gene="fliH"
                     /locus_tag="JNY73_002580"
     CDS             5174..5860
                     /gene="fliH"
                     /locus_tag="JNY73_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY8048552.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQVHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            5860..7233
                     /gene="fliI"
                     /locus_tag="JNY73_002581"
     CDS             5860..7233
                     /gene="fliI"
                     /locus_tag="JNY73_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY8048553.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7252..7695
                     /gene="fliJ"
                     /locus_tag="JNY73_002582"
     CDS             7252..7695
                     /gene="fliJ"
                     /locus_tag="JNY73_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY8048554.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7692..8819
                     /gene="fliK"
                     /locus_tag="JNY73_002583"
     CDS             7692..8819
                     /gene="fliK"
                     /locus_tag="JNY73_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY8048555.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8924..9388
                     /gene="fliL"
                     /locus_tag="JNY73_002584"
     CDS             8924..9388
                     /gene="fliL"
                     /locus_tag="JNY73_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY8048556.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9393..10397
                     /gene="fliM"
                     /locus_tag="JNY73_002585"
     CDS             9393..10397
                     /gene="fliM"
                     /locus_tag="JNY73_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY8048557.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10394..10807
                     /gene="fliN"
                     /locus_tag="JNY73_002586"
     CDS             10394..10807
                     /gene="fliN"
                     /locus_tag="JNY73_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY8048558.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYDVRITDIITPSERMRRLSR"
     gene            10810..11175
                     /gene="fliO"
                     /locus_tag="JNY73_002587"
     CDS             10810..11175
                     /gene="fliO"
                     /locus_tag="JNY73_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY8048559.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11175..11912
                     /gene="fliP"
                     /locus_tag="JNY73_002588"
     CDS             11175..11912
                     /gene="fliP"
                     /locus_tag="JNY73_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY8048560.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11922..12191
                     /gene="fliQ"
                     /locus_tag="JNY73_002589"
     CDS             11922..12191
                     /gene="fliQ"
                     /locus_tag="JNY73_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY8048561.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12200..12984
                     /gene="fliR"
                     /locus_tag="JNY73_002590"
                     /pseudo
     CDS             12200..12984
                     /gene="fliR"
                     /locus_tag="JNY73_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
     gene            13269..13892
                     /gene="rcsA"
                     /locus_tag="JNY73_002591"
     CDS             13269..13892
                     /gene="rcsA"
                     /locus_tag="JNY73_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY8048562.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13936..14178)
                     /gene="dsrB"
                     /locus_tag="JNY73_002592"
     CDS             complement(13936..14178)
                     /gene="dsrB"
                     /locus_tag="JNY73_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY8048563.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            14287..14514
                     /gene="yodD"
                     /locus_tag="JNY73_002593"
     CDS             14287..14514
                     /gene="yodD"
                     /locus_tag="JNY73_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY8048564.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14812..15627
                     /locus_tag="JNY73_002594"
                     /pseudo
     CDS             14812..15627
                     /locus_tag="JNY73_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15624..17318)
                     /gene="yedQ"
                     /locus_tag="JNY73_002595"
     CDS             complement(15624..17318)
                     /gene="yedQ"
                     /locus_tag="JNY73_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY8048565.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGCIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
BASE COUNT         4372 a         4413 c         4470 g         4117 t
ORIGIN      
        1 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
       61 tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg
      121 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      181 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
      241 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
      301 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag
      361 catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca
      421 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
      481 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaacct tgtgaatctc
      541 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctt ttgccagcat
      601 actcttaccc tactttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
      661 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
      721 ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc
      781 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
      841 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
      901 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
      961 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc
     1021 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     1081 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1141 tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     1201 tgtgccttga tctcattatg cataaaggca caatgatcgc gcaaatgcaa tcataattat
     1261 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag
     1321 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     1381 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga
     1441 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     1501 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     1561 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     1621 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     1681 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctagtgtat
     1741 gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     1801 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     1861 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     1921 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     1981 caagcatagt caacaggtta cacaacctac acctgcatag acatcacttc ctgatacgcc
     2041 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     2101 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     2161 gtgcgggcga ctgtttgtgt atcgcttatg cgatcgatcg cggcgtgcag ctgcccggca
     2221 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     2281 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     2341 tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     2401 acgggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     2461 agtttgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     2521 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     2581 gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca
     2641 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     2701 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     2761 tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgc
     2821 gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg
     2881 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg
     2941 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
     3001 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
     3061 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt
     3121 ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc
     3181 tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg aaatatgcca
     3241 gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct attgttggta
     3301 acggtaatat tcacgcctag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg
     3361 aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta
     3421 atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
     3481 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc
     3541 agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca
     3601 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
     3661 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc
     3721 tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg ggcttttctg
     3781 aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg
     3841 gcggagcact gccgttttgg caacagcaag tgtttatcga tcagttgctg gctgccggtc
     3901 gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc
     3961 taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag
     4021 tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta
     4081 accaacgact gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     4141 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     4201 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     4261 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
     4321 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     4381 tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     4441 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc
     4501 ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag
     4561 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
     4621 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     4681 ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt
     4741 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     4801 atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg
     4861 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt caatgatcgc
     4921 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg
     4981 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     5041 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag
     5101 aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc
     5161 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
     5221 gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc
     5281 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggtccacg agcagggtta
     5341 tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact
     5401 ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc
     5461 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
     5521 agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc
     5581 aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     5641 attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat
     5701 gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc
     5761 tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     5821 gcaagaactc tgccgtctgg cagcactagg agtggtgtaa tgaccacgcg cctgactcga
     5881 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     5941 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     6001 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     6061 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     6121 gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc
     6181 aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg
     6241 ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
     6301 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     6361 gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt
     6421 aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt
     6481 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgaa
     6541 ggacgtgcac gctcagtggt gattgccgct ccggcggatg tttctccgct cctgcgaatg
     6601 caaggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
     6661 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
     6721 attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca
     6781 ctggtcgagc gtgccggtaa tggcattagc ggcggcggct cgattaccgc gttttatacc
     6841 gtgctcaccg aaggcgatga ccagcaggac cccattgctg actccgcgcg ggcaatcctc
     6901 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
     6961 attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
     7021 gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
     7081 ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag
     7141 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
     7201 gggctggagc gtattttccc gacagtgtca taacccagga gataacggca gatggcggaa
     7261 catggtgcgc tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgctcgc
     7321 ctgctgggtg aaatgcgtcg cggatgtcag caggcggaag aacagctcaa aatgctgatc
     7381 gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc
     7441 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
     7501 cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa
     7561 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
     7621 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
     7681 aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat
     7741 tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat
     7801 tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtggca acagataagc
     7861 ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt
     7921 tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac
     7981 cgttaaccac cgctcagaca atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag
     8041 acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga
     8101 tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg
     8161 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg
     8221 atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta
     8281 aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc
     8341 cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt
     8401 ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac
     8461 aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag
     8521 tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc
     8581 tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc
     8641 aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
     8701 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
     8761 ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac
     8821 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
     8881 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
     8941 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
     9001 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
     9061 gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt
     9121 gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac
     9181 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc
     9241 acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
     9301 taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
     9361 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
     9421 aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta
     9481 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
     9541 gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt
     9601 tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccatacc
     9661 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc
     9721 tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc
     9781 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg
     9841 aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga
     9901 aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca
     9961 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
    10021 acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat
    10081 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
    10141 tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc
    10201 tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg
    10261 atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggcactctca
    10321 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
    10381 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
    10441 cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga
    10501 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
    10561 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
    10621 agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc
    10681 gctggatatt ctgaacaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga
    10741 taaatatgac gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag
    10801 ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca
    10861 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
    10921 ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc
    10981 gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg
    11041 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca
    11101 ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt
    11161 ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta
    11221 tgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac
    11281 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    11341 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    11401 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    11461 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    11521 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    11581 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    11641 ccggcccgtt gcaggggcct gaagctgtac cgatgcgcat tttgctcccg gcctacgtga
    11701 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    11761 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    11821 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    11881 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    11941 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    12001 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    12061 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    12121 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    12181 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    12241 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    12301 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    12361 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    12421 cgtgcagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    12481 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttat
    12541 cgatcctggc gccatctcaa tatgcccgtt ttagcgcgca tcatggatat gctggcatta
    12601 ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    12661 cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc acccaccaaa
    12721 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgttg
    12781 ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt
    12841 gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc attaatgccg
    12901 ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct ggctgatatt
    12961 attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct aaggattatc
    13021 cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata tttgttttaa
    13081 gctcactcac atatcgcaac atttacttta agacaattcc aggcaaatta tacaacactt
    13141 tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg
    13201 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
    13261 ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg
    13321 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
    13381 accttgccat agcttgtgat tcacagcgtc cttcagtggt gtttattaat gaggactgtt
    13441 tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata
    13501 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
    13561 gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg
    13621 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
    13681 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
    13741 tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
    13801 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
    13861 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttcccaccag
    13921 tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc
    13981 attaaagaac cagatgccga gcgggtagtc ttccagcgca accaggtaca ttgtgccttc
    14041 actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac
    14101 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
    14161 atttcgtttt tctggcagcg ccaagcgcgc gcgagtgctg attttctcga ctgtctatac
    14221 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
    14281 aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat
    14341 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
    14401 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
    14461 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
    14521 aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt
    14581 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg
    14641 ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg
    14701 cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga
    14761 ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca
    14821 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
    14881 tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc
    14941 attctctgta gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta
    15001 caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag
    15061 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt
    15121 ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat
    15181 gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat
    15241 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct
    15301 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat
    15361 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
    15421 ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag
    15481 gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat
    15541 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
    15601 ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg
    15661 gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa
    15721 tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc
    15781 ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca
    15841 gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg
    15901 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
    15961 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga
    16021 atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg
    16081 aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac
    16141 gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggaaaggagt
    16201 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
    16261 ccgcgcacgc cttcgcttag cgtatggacg cgcgccagca caccgtcgaa atggtccaga
    16321 cgttcccagc taacatagcg actgtccata cgaatgcagc cccgcgtgtc atgttccatt
    16381 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
    16441 ttggaagagg tcaaaaatat cagcttactg tcatagagct gatactcacc atcgaggttt
    16501 ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc
    16561 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
    16621 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
    16681 tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
    16741 cgcataaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc
    16801 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
    16861 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact
    16921 aatgcatccg atacgccatt gactggcaga gtgcgccgtc ggttgagttc gatttgccag
    16981 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
    17041 agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
    17101 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag
    17161 atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat
    17221 accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc
    17281 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
    17341 ttttatgatt ctggcataac ccggcaatag cg
//
LOCUS       DADLZM010000083        17341 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLZM010000083 DADLZM010000000
VERSION     DADLZM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.083"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNY73_002596"
     CDS             complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNY73_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY8048566.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1337..1903
                     /gene="efp"
                     /locus_tag="JNY73_002597"
     CDS             1337..1903
                     /gene="efp"
                     /locus_tag="JNY73_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY8048567.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1955..2080
                     /gene="ecnA"
                     /locus_tag="JNY73_002598"
     CDS             1955..2080
                     /gene="ecnA"
                     /locus_tag="JNY73_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY8048568.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2191..2337
                     /gene="ecnB"
                     /locus_tag="JNY73_002599"
     CDS             2191..2337
                     /gene="ecnB"
                     /locus_tag="JNY73_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY8048569.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2513..2742
                     /locus_tag="JNY73_002600"
                     /pseudo
     CDS             2513..2742
                     /locus_tag="JNY73_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2739..3272)
                     /locus_tag="JNY73_002601"
     CDS             complement(2739..3272)
                     /locus_tag="JNY73_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8048570.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3361..4494)
                     /gene="blaEC"
                     /locus_tag="JNY73_002602"
     CDS             complement(3361..4494)
                     /gene="blaEC"
                     /locus_tag="JNY73_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY8048571.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="JNY73_002603"
     CDS             complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="JNY73_002603"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8048572.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="JNY73_002604"
     CDS             complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="JNY73_002604"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8048573.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5333..6067)
                     /locus_tag="JNY73_002605"
     CDS             complement(5333..6067)
                     /locus_tag="JNY73_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY8048574.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="JNY73_002606"
     CDS             complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="JNY73_002606"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY8048575.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8193..9170
                     /gene="epmA"
                     /locus_tag="JNY73_002607"
     CDS             8193..9170
                     /gene="epmA"
                     /locus_tag="JNY73_002607"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY8048576.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9389..10891
                     /gene="yjeM"
                     /locus_tag="JNY73_002608"
     CDS             9389..10891
                     /gene="yjeM"
                     /locus_tag="JNY73_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY8048577.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="JNY73_002609"
     CDS             complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="JNY73_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY8048578.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14375..15343)
                     /gene="psd"
                     /locus_tag="JNY73_002610"
     CDS             complement(14375..15343)
                     /gene="psd"
                     /locus_tag="JNY73_002610"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY8048579.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="JNY73_002611"
     CDS             complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="JNY73_002611"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY8048580.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16587..17132
                     /gene="orn"
                     /locus_tag="JNY73_002612"
     CDS             16587..17132
                     /gene="orn"
                     /locus_tag="JNY73_002612"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY8048581.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4002 a         4470 c         4547 g         4322 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc aggcaatttt
      241 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
      301 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      361 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      421 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      481 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      541 ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct
      601 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      661 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      721 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      781 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      841 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      901 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      961 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagtggg
     1021 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
     1081 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
     1141 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
     1201 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1261 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1321 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1381 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1441 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1501 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1561 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1621 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1681 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1741 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1801 cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg aagtcatcaa
     1861 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1921 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1981 tttgcttgcc agcacgctgc tcacgggctg taacaccgct tgcggtttcg gcgaagacat
     2041 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     2101 atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     2161 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     2221 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2281 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca
     2341 ataacggtac gacagcagtg tcgtgccgtt tgttttttct gcgatcgtca taaaggtaat
     2401 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2461 cctgcaaacg cctcttttca ccggggacgg ccccaaattt ccggagcctg atatgtcctg
     2521 gattatctta gttattgctg gtctgccgga agtggtatgg gccgttggcc tgaaatatac
     2581 ccacggcttt agccgtttga tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2641 accggcattg tgctgctcgg tgagtccgct aacccgatgc gtctggcgag tctggcgtta
     2701 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2761 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2821 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     2881 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     2941 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3001 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3061 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3121 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3181 actacggtca cgccacgcgg cggcgtagga gaactacagg caacgaccag aaatgcagct
     3241 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3301 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3361 ttactgtaga gcgttgagaa tctgacaggc ggcggcgact ctctctggat tgggatagtt
     3421 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3481 aaatccgccg gtcgcccccg ttttatgtac ccatgatgcg cgtactgcag gagttggggg
     3541 cttaatcgct tttaccgggc gtgctgccag tgcaatttta ttgtcactgc cgttaatgat
     3601 gctgtcagga ttgaccggcc agtccagcat ttcccagccc aggccctgat acatatcgcc
     3661 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagcgttt tctcattgat
     3721 atcaaggggg ggtaaattgc tttgcaccca gcgggccata tcttcaatgg tcgacttcac
     3781 accataagct tcagcatcta acgcccctgg cgaaacatgc actgccttac cttcgcgata
     3841 tccccaggcg taattctttt cttctggggg aggtacatta atccacgtat ggttgagttt
     3901 gagtggctgg aagacacgag tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     3961 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggagc
     4021 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacttcatc
     4081 cggcacctgc aatggcaggc cgccagcggt gtaggtcgca agatgtaata gtgtgatccc
     4141 attccactgt ttagcggtaa gttcaggcca gtattttgtt gcgggatcgc ttaacttgat
     4201 ttcccctcga gcaatagcgt cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4261 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg catagcccca
     4321 ggtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4381 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4441 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acataaggtc
     4501 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatgcgttag
     4561 attgtaacga caccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4621 catttgcccg caggcacgtg gattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4681 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4741 gcgaacgcca gaacgcgctc gtagctcagc gcatcacccg gaaacagccc cagtggcagc
     4801 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4861 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     4921 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     4981 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     5041 cgctttcggt gccagttcaa accaggtttt ggtgtgcagc agagctgccg tcagagtgat
     5101 caggttaatg atcacgataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5161 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5221 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5281 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5341 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgc tgaatggccg
     5401 cagccggatc gacgtgtttc gggcagactt cggagcaata gcccacgaaa gtacagctcc
     5461 atacaccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5521 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5581 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5641 actttgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5701 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5761 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt catactgtcg gtgtaatcac
     5821 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     5881 aaatcgccat acggcaggac cagcggtaac tcaggtccgg tgccaggttg tctttgatgt
     5941 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     6001 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6061 cagccattcg ccttctcctt attattggct gcttccgcct tatcggctgc atccgcttcg
     6121 ccaccgtaaa cgcgtttagc tggcggcagc gtagtaatct tcacgtcgct gtactccagg
     6181 cgagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgagaaagtt gacgtcgtca
     6241 cgctcggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6301 gcggagtgcg ccatacattc agcaacgttc agaccgtggc tcagttcaat ggtgtagagc
     6361 aggtcggtgt tgaacacgct ggaggtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6421 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6481 ccttcttcca ttgccaggcc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6541 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6601 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6661 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6721 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6781 tcgataccgc ccatggtgta gtgtgcagtc ggacgtaccg gaatcggttc tttaaccgga
     6841 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     6901 ttctcgccga ggtgacgcag gtcgagataa accacatcac cacgcggcgt ggagatagtg
     6961 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     7021 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7081 taacggtagc catttttgtt gaccagaata ccgccttcac cacggcagcc ttcggtcatc
     7141 aggataccgg aacctggcag accggttggg tgatactgaa cgaattccat gtcacgcagc
     7201 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7261 ttgtaacgat aaacgcgacc cgcaccgccg gtagccataa cgaccgcgtt agcacggatc
     7321 tgcaccaacg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7381 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7441 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtgcgc
     7501 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7561 gggcatcccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7621 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7681 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7741 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7801 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7861 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     7921 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     7981 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     8041 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8101 tgcccaccgc tgtttgattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8161 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8221 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8281 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8341 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8401 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gctggttgtg
     8461 ggccggtatt ccagctgtgc cgcagcttcc gtaatgaaga gatggggcgt tatcacaacc
     8521 ctgagttcac tatgctggag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8581 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8641 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8701 gggaagtcgc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8761 cgttgctaca attgctgttt acctttggcg tagaggcaaa tattggtaaa gaaaaaccga
     8821 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     8881 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     8941 atgaattaac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     9001 cggcgcgcgg tttgccgcag caccccattg accagaatct gattgaagcc ttgaaagtcg
     9061 gtatgcctga ctgctccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9121 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9181 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9241 ttttccgtta aaggccacaa tttaagactg gatatttgat atcatcaagg tatcaattct
     9301 gtattggttt ttacgctgcc tgctcaatgt tgcgcagtgc agttcagtgg gcacgtgtta
     9361 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9421 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9481 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9541 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat
     9601 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9661 gttttcctct tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc
     9721 aacattcctc tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac
     9781 gcaggtggtt ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9841 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
     9901 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
     9961 ggatattaat ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc
    10021 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10081 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10141 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10201 attaagtaat ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg
    10261 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10321 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10381 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10441 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt
    10501 ggttactgtc ttcattctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10561 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10621 tttcccgttt tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg
    10681 tatgtcggca atgattgcga cggtggttgt cgttctggtg gtgacatttg cgaacgtctt
    10741 caccattatt cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg
    10801 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10861 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    10921 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    10981 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    11041 attacaaact tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt
    11101 taccgttgag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11161 cgtggaagcc tgccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11221 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga
    11281 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11341 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac actacgcgcg
    11401 tgaccgagtc agagagcgac cagttgataa actgcgcggt aataaacgcc ttgttcggca
    11461 cgattatctc tttacggtcc cagtcgctga tggtggtggc gcgggtgtta atttttgtca
    11521 cgctaccagt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
    11581 ggatgatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
    11641 caccgagcgc ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc
    11701 cgaccagccc gccaatcagc atcagcagat attttgtgat ggtggtgatg gcgtaacccg
    11761 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc
    11821 gcaccagctg cgtggtgatg ataaatacca gaatggcaat cagcaccgca ccgagggtaa
    11881 ttggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    11941 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa cgcagggcga
    12001 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
    12061 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
    12121 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12181 gcctgcgacg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12241 accagatggc aaccgaggtt tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac
    12301 ccaccgccga cgccagaatg gcaaccagtg gtgcgccaat catcatgttc cacagcatat
    12361 ggttggtaat gttgtcgccg ctgccttctt tgttgagata caacggaata ccggcttttt
    12421 tcaggcttag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12481 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc
    12541 cgatgctcat caggtagtag cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    12601 gagcgataaa caagccgttc gggcgggcaa aggtggcgca aatcatcact acccacagca
    12661 gcggcacggt ggccgttaca ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct
    12721 cgcgcaagcc gtaacccagc gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg
    12781 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    12841 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    12901 gaatcaacgc gccaaacagc ggcaaaattg tctctttgct ggtcagcatc atcacactgg
    12961 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13021 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg
    13081 ttgcttcgtt cacctctttc agcgcatcct ccagttgccc gttggagact ttcagcttgg
    13141 tcagttccaa tagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13201 tgcgcagttg tgcttccaga atacggtttt gctcggcggt cagcggctga ccgtcggcct
    13261 gatggatttg ccgtagcagc ggctgtttat taagtaaatc ctcataacgt aaccgttgca
    13321 cacgcaactg cgccatttcg gtatcaagct gttgtggttt tggcatttct ggtagccgtg
    13381 ccacctgcgc ccgcagcgct tcgccgagca ggttggacga tcccagccat tgcgactgtt
    13441 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    13501 gcgaggcaac gagctccatc cgctgcgcct gttgattcag cgctgccgat agttcgcggt
    13561 taattttgaa ttgcgcgacg atatctttcg gcaaatcggc actgttttct gccagtaatt
    13621 cggtactttc cagcgcccgc tccgcctcaa gctgacgttg gctattcaac tgattacgta
    13681 agccctgcaa atacgcatcc agttgctggc tctctttttc cgccagctct gagcgtaagc
    13741 gcgctaattc ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg
    13801 ccttaagacg tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat
    13861 tgccggtaag cgttcccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    13921 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    13981 gggcctgacg gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg
    14041 cgtcggtaga cattcccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    14101 cgcgcagagt agcggagagt ttcggataat tatcgataac ttgctgatat tgtttgatgc
    14161 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14221 cttccggctg tgcgggtttc gccgcttttg cctgctccag ttcctgagtg atttgtttgc
    14281 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    14341 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    14401 ccaatggcct ggcgtcgtgt tctgcttcga tctcttcagc aggaagtggg gcgggttcag
    14461 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    14521 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    14581 tggaaccgag tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    14641 tttccccggc aggccaggtc cagcgcttga tgataccttc gcgcggcggc gtaatggtgc
    14701 ccgcccagac cgtctcaatg ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg
    14761 ggccaaattc ggtatcgaaa aggcaaatca cgcgttcgtt acgggcaaac agattcggca
    14821 cgttctgagc ggtgagatgg ttaacggaaa agagatcgcc cggcacgtag atcatctcac
    14881 gcagaatacc gttgcacggc atgtgtacgc gatggtagtc acgcggggag aggtaagtgg
    14941 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    15001 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15061 ggctgataac gccatcggca ggcatgacca gtacattcgg atcggtatcg attgggcgta
    15121 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15181 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15241 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    15301 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    15361 tgttttgtcg ttcctgatcc ggccaacatg ccggatcctg aaaaaaaggg gacgattcta
    15421 acgacggtta gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct
    15481 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    15541 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    15601 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    15661 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    15721 agtgatacag ccgagcggcg gtcgtggtgt gctgaccgag ccccgagttg tcagagacat
    15781 cgttggtcag gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttaccga
    15841 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttca
    15901 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatgttg cggtagatat
    15961 ccatctgctc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16021 cgataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16081 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggctatgg
    16141 gtttcacgcc gtcgtagaaa tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt
    16201 ccacgatccc tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16261 ggtcgccggt taccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    16321 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    16381 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    16441 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtactg
    16501 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    16561 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    16621 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    16681 tgaccgatgc caatctgaat attctggcag aagggccgac cattgcagta catcagtccg
    16741 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    16801 tagagcgcgt gaaagcgagc acgatgggcg atcgggaagc tgaactggca acgctcgaat
    16861 tcttaaaaca gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggacagg
    16921 accgtcgttt cctgtttaaa tacatgccgg agctggaagt ctacttccac taccgttatc
    16981 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt
    17041 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    17101 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17161 atccacttgc ggcggcgtga ttttgacgct aaatcgaacg tcttgctgat aatttcagca
    17221 cttgagataa aaacgcaaaa aaaacttttt tggggggttg cagagggaaa gatttctcgt
    17281 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    17341 a
//
LOCUS       DADLZM010000084        17239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLZM010000084 DADLZM010000000
VERSION     DADLZM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.084"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..170
                     /locus_tag="JNY73_002613"
     CDS             <1..170
                     /locus_tag="JNY73_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY8048582.1"
                     /translation="SRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAV
                     FGGELTLVQLQ"
     gene            complement(171..446)
                     /locus_tag="JNY73_002614"
     CDS             complement(171..446)
                     /locus_tag="JNY73_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY8048583.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(467..703)
                     /locus_tag="JNY73_002615"
     CDS             complement(467..703)
                     /locus_tag="JNY73_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY8048584.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNY73_002616"
     CDS             complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNY73_002616"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY8048585.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPVPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNY73_002617"
                     /pseudo
     CDS             complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNY73_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNY73_002618"
                     /pseudo
     CDS             complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNY73_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6356..6853
                     /locus_tag="JNY73_002619"
     CDS             6356..6853
                     /locus_tag="JNY73_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY8048586.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6950..7648
                     /gene="proQ"
                     /locus_tag="JNY73_002620"
     CDS             6950..7648
                     /gene="proQ"
                     /locus_tag="JNY73_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY8048587.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7668..9716
                     /gene="prc"
                     /locus_tag="JNY73_002621"
     CDS             7668..9716
                     /gene="prc"
                     /locus_tag="JNY73_002621"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY8048588.1"
                     /translation="MSMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLERSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9908..10789
                     /gene="htpX"
                     /locus_tag="JNY73_002622"
     CDS             9908..10789
                     /gene="htpX"
                     /locus_tag="JNY73_002622"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY8048589.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10835..12208)
                     /locus_tag="JNY73_002623"
     CDS             complement(10835..12208)
                     /locus_tag="JNY73_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048590.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12385..13176
                     /gene="kdgR"
                     /locus_tag="JNY73_002624"
     CDS             12385..13176
                     /gene="kdgR"
                     /locus_tag="JNY73_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY8048591.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13319..13558)
                     /locus_tag="JNY73_002625"
     CDS             complement(13319..13558)
                     /locus_tag="JNY73_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048592.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13717..13860
                     /gene="mgrB"
                     /locus_tag="JNY73_002626"
     CDS             13717..13860
                     /gene="mgrB"
                     /locus_tag="JNY73_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY8048593.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13935..14222
                     /locus_tag="JNY73_002627"
     CDS             13935..14222
                     /locus_tag="JNY73_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048594.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14892..15035
                     /locus_tag="JNY73_002628"
     CDS             14892..15035
                     /locus_tag="JNY73_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY8048595.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15048..15257
                     /gene="cspE"
                     /locus_tag="JNY73_002629"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15048..15257
                     /gene="cspE"
                     /locus_tag="JNY73_002629"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY8048596.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15423..16232
                     /gene="rlmA"
                     /locus_tag="JNY73_002630"
     CDS             15423..16232
                     /gene="rlmA"
                     /locus_tag="JNY73_002630"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY8048597.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNY73_002631"
     CDS             complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNY73_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY8048598.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4554 a         4171 c         4346 g         4168 t
ORIGIN      
        1 gtagccgctc gcgattaggt gaacgcccaa aagggcaggg aattacaggt gctgatcatt
       61 tctggtttgg tcatacaccg ttgcgacatc gcgtggatat tggcaacctg cattatattg
      121 ataccggtgc tgtctttggg ggcgaactga ctcttgtgca actgcaataa ttaaaaatca
      181 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
      241 ccatggcgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
      301 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
      361 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
      421 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
      481 taatccaggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
      541 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
      601 ggtcagattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
      661 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
      721 agctgctgat tgtggtacga ccagtccgat cgcacaattt ttcagcctag ttgaggccgc
      781 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
      841 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
      901 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
      961 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa
     1021 aagcgtttac attgtcgggg gaggcatggg caataaccgc ttcatgctgc cagcgataac
     1081 ctttgttatg cgtttcggca agtttaatcc ctaagcgaga aaatcggact ttaccgatca
     1141 gggcttcaat gcctaccggg aacaaccaca gttctttgtc gcgttgccag agtcgcaggt
     1201 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtccg gcttcgcgat
     1261 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tgggacgggt aaggcgggga
     1321 tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
     1381 aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc accagggaag agatcgccaa
     1441 gtggtaaaaa ctctacttcg tcggggtaag tctctttcag ccaccggcaa acggcttcgt
     1501 tttcttcccg gtttaaggta caggtcgagt aaaccagcgt accgccagga cgtaatgcat
     1561 gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat ttcctgattg ctttcgggtg
     1621 accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
     1681 ccagcaaaat ggcatcaaac atttctggca ctgacgcacc aaacacgcgg ccaccaaaat
     1741 gtgtgagcgc aacattactg atgccacagc ggctgatatt ggcatgtaac acttttaccc
     1801 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
     1861 gcgtcgtttt tgagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
     1921 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
     1981 ttaaatgctc ggtggtacta cccaatggca atgcatcttc attgtcgcgt tcaatccaga
     2041 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaattgca
     2101 ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
     2161 gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg
     2221 tcagaaacgc gtccgggaaa taaacggcgt gttgggccac gagcatgtac cactggcaaa
     2281 aaaacaggcg cgcagtttag cataaacgct ccggcgcagg cacgccggag cagtgggcat
     2341 tatttgggaa gcgcagttcc ccattcacgc cactctttcg gttcactttc ctgcaacagg
     2401 aagtgtttgc cttcctgggc tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg
     2461 atgccgccac ggataaattg gttaaaggtg ccggttttca ctacgccgcc cgtcagacca
     2521 aagtccagac tgtaacccga tgccaaccag aagacggaat tgttacgcac caggtgttga
     2581 tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt
     2641 ccggtaaccg tacctacttc cagaccacgg aacagcacag gcgtaccgat gcctaacgaa
     2701 ccggcttccg gcgcttcaac aataatgctt aaaccatcta agtaacgcga atcagtaatg
     2761 gtggcctctt ctaattcaaa gtcgcgacga ggattgccac ggcctggttc gacgttgata
     2821 tacggctgga ggatagtatc tagatgctca acgccagctg ccgagatttg cggtgtgacc
     2881 actgagaagc gcgtaccacc gcgagcaaag gtctggacat attccggata gagcaccgcc
     2941 tttgcctgta cttcattgcg tgcggtaatc agatccagcg tctggatttg cccgatatca
     3001 ataccgagat agcgaattgg catcccgacc gccagttttc cggcatcgaa agcgtgaagc
     3061 gtaatctgcc tgccaaccgc acgggccgct gtttcggaag catacagaat tcgcttgtcg
     3121 cctttacgct gactggcgct ggcaccactg aggttatcga agctaatggc cccctttaat
     3181 gctctggaga gcggggatgc ctgtacggtc aggccactac cattcagctg aactttcgcc
     3241 ccgccttctg cctagaacac gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg
     3301 tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg taataacttc accaacttca
     3361 aatttacggt acagcactac cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa
     3421 ctcacggttg tggtgggtaa atcgctaagg ctgttctcca gcgctttttc cagattggca
     3481 tacagtggat agctggcttt catctcgcct ttatcgcccg gcagaatacg tatcccgccg
     3541 ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc catccagccc caccttcacg
     3601 tcgacacggc tgttgacgac aaatttgcta tcgcctttta ccagttctcg atgctgaggc
     3661 tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt tacgatcgat aacctggcct
     3721 acctgcacgc cgtgaagaat gagcggctga cccgcatcaa taccgtaact ttccggtgct
     3781 gtcagggtca gtgtcagaac gtccggctct tgcagcaggg ctttttcgcc cggcacaaca
     3841 acgaactctt tgcgtggctc gccatcgccg ggcaccaact caaaggtttt tccggtcagc
     3901 agggcgctga gattagcatc gctaagggat aatttcgggt tgcgtaattc tatgcgggta
     3961 ttttcacgaa gcagggtaac gacgctggga tcaacggtca tttccccagt gactttacca
     4021 ccaggattta aatccagttt agtcagctgt ccgacttcca gcccctgata cattaacggc
     4081 gtcgagtcgg cggttaatcc tgctccactc ggcagttcca gttttattat tactccacgc
     4141 tggctgtggg ccagatcttc atacagacca aaggtatctt ccgcctcggc aggtttcgac
     4201 tcttcaggtg aatcgaaggc aatcgcaccg ttaaccagtg ccgccagact ttccagtttc
     4261 accttcgcgc cactgatact gacgttggca tcaacgccgg aaacgttcca gaaacggcta
     4321 ccttttttca ccagatcggt aaaacgccgc tcgatcagga catcaatcac cacgccttgc
     4381 ttgttgggat tgatggcata gtcgtagact tttcccaccg ggatcttgcg gaaatagacc
     4441 aatgaaccgc tgttcagcga accgagatcg ggggcttgca ggtggatcat cagatcgcca
     4501 ttgtccagcc gatatttcgg ttgggtatcg agcgcgacaa agtgatcctg ctctttacct
     4561 ttacccggca tcatgccgat atagttccca ccgacgaggg cgtccagccc ggagacacct
     4621 gccaacgatg cttttggcgt catcagccag aactgagtct cttcgcgcag cgcatctttc
     4681 atatcggact tgatgctgac cttgacttca atcttacgaa gatcgtcgct gaggctgata
     4741 tcctgcactg ttccgacttc aacgccctga taacgaacag gcgtacggcc cggaacaata
     4801 ccatccgccg acataaagtc gatggtgacg gtattacccc ggtcctgata actgtcccaa
     4861 atcagccaac cggcaatcat tagcgcgatg aaaggcagca gccagaaagg tgagatacgg
     4921 cgtttatttt taatctgcgc ttcagtcgtc gaagcgggcg tttcctgact catgtgcatc
     4981 ccaaagtaag cggctgtcca gccattccac agcaagaata gtcaaaatta cggcagcgcc
     5041 aaaataaaac gcagccggtc ccatagtaaa agcgaggatc tgatcgcgat taatcagcga
     5101 catggttaaa gatatgacaa acaggtcgag catcgaccag cgtccaatcc aggtcaccat
     5161 ccgcagtaac agaatgcgtg tgcgtaaacc ttgctggcat ttaaaatgaa tgctcaacag
     5221 taaagtgaac atgacgatca ctttagtaaa cggtaccaga atactggcga taaacacgat
     5281 tcccgcaacc gcaatgttgc tacttgccag cgacataatt ccggaaagaa tcgtatcttc
     5341 ctgccggcca ccattcagat aaatgataga aataggcaat aggttggctg gtaacaacaa
     5401 aacgattgac gctaacagcg ccgcccagca tttttgcaaa ctatgacgac gacgcaggcg
     5461 tagcgggata tggcaacgcg ggcagagacc acgctgatca ggatagccgg taaaatggca
     5521 cccaagacag acacgaagtt tctcgtccct acgcgtagcg gggcgctgcg gataatatcg
     5581 ctcccacagc tcctcaacat taagatgtga caacgtcacc gtcgttaaaa tcaccaacgc
     5641 cacaaaagag aacaagccga cacccgcctg gatatgggca taatcctgta ccttatatag
     5701 aagcaacgcc aatgccgacc aggtagatat ccagcattac ccactcttta agtcgctcaa
     5761 gcatcagcag taccggacgt aaattcattc ccagtcggtt accaaaccat aaatatgcta
     5821 tggaggacac cagaatgagg ggggcaccaa taacgcagaa aaagaccatt gatcccgtta
     5881 tcgcatcgcc ctgtttggtc atttgccaga tgccttgcat gacgttggcg tcaatacgaa
     5941 tgcctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcata agcaacagca
     6001 tggtgaaggc cattgccgcc aggcgcgtta gcgacaagtc gcgcccgtcg cgaatttttg
     6061 cctgacagcg cggacaatag gcactttgat gagaatttat ctcgggcagg ctaaacagca
     6121 tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg attgccctca
     6181 ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg tgccaaatgt
     6241 aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat taacgcattt
     6301 aatgcttatt ttaataggct ggcagttcca ggtaagacaa ccaagtgatt aaataatgaa
     6361 caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg cgggagaaac
     6421 cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tacgagcgtc tcactgacgt
     6481 taactgggca ggtttttatt tgcttgagga cgatacactg gtactcggac catttcaggg
     6541 caaaattgcc tgtgtccgga tacccgtcgg gcgcggggtg tgcggcacag cggttgcccg
     6601 caatcaagtg cagcgtatcg aggatgttca cgcgtttgac gggcatattg cctgtgatgc
     6661 ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta ttggtgttct
     6721 cgacatcgat agtaccgtct tcgctcgctt tacagacgag gacgagcaag gcttacgtca
     6781 gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat tctttgcgag
     6841 cgtcgcagga taatcaacgg ataacgtagc atttactggt agcgtcatta taatgacgcc
     6901 tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca tggaaaatca
     6961 acctaagttg aatagcagta aagaagtaat cgcgtttctc gccgaacgtt ttccccactg
     7021 tttcagtgcg gaaggtgaag cgcgtccgct gaaaattggt atttttcagg atttggtcga
     7081 tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt tacgtctcta
     7141 cacttcgagc tggcgttatc tttacggtgt taaacccggc gcaacgcgtg tcgatcttga
     7201 cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca agcagcttga
     7261 agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa aacgcgaagc
     7321 tgccgcagct gctggtgaga aagaagacgc accgcgccgc gaacgcaagc cacgtccgac
     7381 cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc cggtagagaa
     7441 agcgccaaaa acagtaaaag cacctcgcga agaacagcac accccggttt ctgacatttc
     7501 agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga tggatgccac
     7561 cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta tgtctttgat
     7621 tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg agcatgtttt
     7681 ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc gctgtagaag
     7741 atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat gcgacggtaa
     7801 gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac ctcgatcagg
     7861 cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc cacaatgtgc
     7921 tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc gatgaactgc
     7981 gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag cgccgttttg
     8041 aacgttacca gtacgctttg tcggtactgg aaaagccgat ggatttcacc ggcaacgaca
     8101 cttataacct tgagcgcagc aaagcgccct ggccgaaaaa cgaggctgag ttgaacgcgc
     8161 agtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctgacagga aaaacggata
     8221 aagaaattcg tgaaaccctg acacgccgct acaaatttgc cattcgtcgt ctggcgcaaa
     8281 ccaacagcga agatgttttc tcgctggcaa tgatggcgtt tgcgcgtgaa atcgatccgc
     8341 ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg agtttgtcac
     8401 tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc aattcgatgg
     8461 tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa attgtcggtg
     8521 ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat gatgtggttg
     8581 ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct gctggtaaag
     8641 ggaccaagac ccgtactgta acgttgaccc gtgaacgtat tcgtctcgaa gaccgcgcgg
     8701 ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat attccgggct
     8761 tctatgtggg tttgacagac gatgtcaaag ttcaactgca gaaactggaa aaacagaatg
     8821 tcagcagtgt gatcatcgac ctgcgtagca atggcggtgg ggcgttgacc gaagcggtat
     8881 cgctctcagg tctgtttatt cctgcgggtc ccattgttca ggtccgcgat aacaacggca
     8941 aggttcgtga agacagcgat accgacgggc aggtgttcta taaaggccca ctggtggtac
     9001 tggttgaccg tttcagtgct tcggcttcag aaatctttgc cgcggcaatg caggattacg
     9061 gtcgtgcgct ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag caataccgtt
     9121 cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg ggttctgtgc
     9181 agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt aaaggcgtaa
     9241 caccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag aaattcgaag
     9301 ataacgcgct gccgtgggat agcatcgatg ccgcgactta tgtgaaatca ggagatttaa
     9361 ctgcctttga accggagctg ctgaaggaac ataatgcgcg tatcgcgaaa gatcctgagt
     9421 tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag cgcaatatcg
     9481 tttctctgaa ctacgctgtg cgtgagaaag agaataatga agatgatgcg acgcgtctgg
     9541 cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaag ctggatgatc
     9601 taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat atcgcactcg
     9661 atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgctcccgtc aagtaatatc
     9721 aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg taaatcagat
     9781 gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga cttgaaaata
     9841 gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact gaggtaaaaa
     9901 gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa tggtcgtttt
     9961 cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc tgatgatcat
    10021 ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca aatggatggc
    10081 attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg aacgttggct
    10141 ggtcaatact gtagcaaccc aggctcgtca ggcggggatc gctatgccgc aagtggctat
    10201 ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg cctctctggt
    10261 tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg cggtaattgc
    10321 tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga ttcagggcgt
    10381 ggtgaacacc ttcgttatct ttatttcccg tattctggcg cagcttgccg cgggttttat
    10441 gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct actttgcggt
    10501 tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca tgtggttctc
    10561 gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc gcgagaaaat
    10621 gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa ccagcatgat
    10681 ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgttcatga ctcacccgcc
    10741 gctggataaa cgtattgaag ctctgcgtac gggtgaatac ctgaagtaat atgaagtaaa
    10801 tactaaaaag cgcgtcctga tcgacgcgct ttttttatgc cctggctcgt ggctgagtga
    10861 tacgtaaacc actgacacag gcagcaatca ctgccagaat agccgcagcc atcagcgaga
    10921 cgtgcgtacc attatcacca aactgattta gcatcagcgc caccagcgcc gcgccactac
    10981 tctgacccag tagacgagcc gttcctaaca tgccactggc cccaccgcta cgttcgcgcg
    11041 gagcggaggt aataatggtg tggttattgg gtgactggaa taagccaaat ccagcgccac
    11101 ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc agaaccaggg
    11161 aaaaaagccc cgcagccatg atgaacaacc ctaaagcacc cagcaatcct gcatgtacgc
    11221 gttcaatcaa atagcctgcc agcggagcca tcaccatcgt tgctaacggc cacggtgtca
    11281 gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa aagggcaggg
    11341 aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata gaaagtgaaa
    11401 acagcgggat acgcagtaaa tccaccggta gcagcggtac gggaagagaa agctggcggc
    11461 gaataaagaa aataccaaca acaaccatta ccaccagttc cgcagcaatt aacgtcagcg
    11521 attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt aacgcgttca
    11581 tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccattg ggtggcagaa
    11641 aacgcatcgc cagaagcagg gcgataatac ctaacggtac gttgattaaa aataaccatt
    11701 tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca gaagagacgg
    11761 caacaataaa cgagtttatg cccatccctc tccccagaaa acgttgtgga tagatcaggc
    11821 ggataagtgc ggtattaacg ctcatcaacg ctgcaccgcc gaaaccttgt atgacacgcg
    11881 caagggtgag catttgcagc gaatcagaaa gggcgcagaa cagtgaagac aacagaaaaa
    11941 cgaccagacc gcatttataa atacgtcgat agccaaacat atcgcccaga aacgaaaacg
    12001 agagcaggga gatgacaatg gcgatttgat aggcgttcac tacccagatg gaactggctg
    12061 gtgtggcatg aaggtccgtg gcgattgttg gcagggcgac gttggcgatt gcgccgtcaa
    12121 ggacagccat cgaaatacca atcacaatgg ttaatatcgc accgtatcgc tggggcaatg
    12181 gcaggccgtc ggcctgaact tttggcatag gaattttata tctttggtga ataatcaaca
    12241 ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa ctaaccggca
    12301 aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc tgatttttat
    12361 aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca gcctgattct
    12421 gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga gcgcgaaata
    12481 gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta tcgcttttta
    12541 cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa atattccctg
    12601 accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt aattcgtagc
    12661 gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca cctcggcgca
    12721 ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa tttgcgcatg
    12781 tattcacgga ttgggcgtcg taatccgctg tacagcaccg cgattggtaa ggtactgctg
    12841 gcatggcgcg atcgcgatga agtgaagcaa attcttgagg gcgtggagta taaacgcagt
    12901 accgagcgga ccatcaccag tacagaagcg ttattacccg ttctggacca ggtgcgcgag
    12961 caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat tgcggtaccg
    13021 gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc gacgttgcgt
    13081 ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc gcgcaaaatt
    13141 tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc tgtcggaata
    13201 tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg cgttatatta
    13261 aacgcgcttg ctataagaat attttactca tgagtgagaa tctggttatt tattaccctt
    13321 aaccattatc gaccacgata ttgcttttac gtaacagtgg gcaatctgtt atccccaaga
    13381 aaccactttt agtgtgcaag tattgtaccg tgctggtgcc tctggcagtc agataggtac
    13441 attgcaaacc taatcctgcg gcattctctt tgcttccaat caaaacgcca tatccgctga
    13501 gtaataatcc tatccatacc agtgctatca gcataactgt gcgaatgatg aatcgcatta
    13561 caatctcttc tctttttatg ttcgcttaat cgtagcggca atatgcgctg aagcaagcta
    13621 ctcattccga aaaagcacga atatcgacat agttaggcgc tgtttaacta acgcatgcta
    13681 gtttaatggc ataaggtagg tgaagcggag attggagtga aaaagtttcg atgggtcgtt
    13741 ctggttgtcg tggtgttggc ttgcttgctg ctttgggcgc aggtattcaa catgatgtgc
    13801 gatcaggatg tacaattttt cagcggaatt tgtgccatta accagtttat cccgtggtga
    13861 tatcattttt tcagcactag attttcttcc tctccagcag tggtagaata gcgcctctac
    13921 tcagaggtgg tgaaatgaac gaagttgtaa attcaggcgt gatgaacatt gcgtctttgg
    13981 ttgtatcggt ggtggttctt cttatcgggc tcatcttgtg gttttttatc aatcgtgcca
    14041 gttcacggac taacgaacag attgaactgc ttgaggcgtt gctggatcag caaaaacgtc
    14101 aaaatgcact gttacgtcgt ttgtgcgaag caaacgaacc agagaaagca gataaaaaga
    14161 ccattgagag tcaaaaatcg gttgatgacg aagatattat tcgcctggtc gccgaacgat
    14221 aatccttcct caatcttgag taaccccgtc gttgaagggt tactcaattc atcctgatta
    14281 tacgcactgt catggtgcaa ctgcttcaaa ttaaaactct ggtctgactt caaataatta
    14341 tcatattatt tatttgcgta acgggatatc agaaaaagat attttcctca ttaatttatt
    14401 aggatgttta catcagattt gtgattaagc gtggtattat ttattagcga aacgtttctc
    14461 tcttgatttt tttgctcact catcaatttt tcttatttta aatttacaat ccctttgggg
    14521 attgacttct ctttagggta attgatagcc gttaactgac tgttttatga gaaaaagtga
    14581 tataactttt tattcattgc atagcagaaa atgtgatatt gcacgcacta tgtaataact
    14641 tctccctctg gcctggaaca actgaactta ttgaactatg ttagaaacta cgccagttta
    14701 agtatctgcc tgaactggca aggttaagca caatgatata tcggcgcgta ttccgttgca
    14761 taagtgtgca aaaaaagtgg aagacgtatc gagatttgtg cgtctgatcg agacatgttt
    14821 aaaaatggct tgccataatt aacgttgtat gtgataaaag atttcgggtt aaacgaggta
    14881 cagttctgtt tatgtgtggc attttcagta aagaagtcct gggtaaacac gttgacgttg
    14941 aataccgctt ctctgccgag ccttatattg gtgcctcatg cagtaatgtg tcagttttat
    15001 ctatgttatg cctgcgggcg aagaaaacaa tctaaggaat ttttcaaatg gcaaagatta
    15061 aaggtcaggt taagtggttc aacgagtcta aaggttttgg cttcattact ccggctgatg
    15121 gcagcaaaga tgtgttcgta cacttctccg ctatccaggg taatggcttc aaaactctgg
    15181 ctgaaggtca gaacgttgag ttcgaaattc aggacggcca gaaaggtccg gcagctgtta
    15241 acgtaacagc tatctgatcg aatccactga tctgaagtgt gaatacgctt caatctcgct
    15301 ataaagcctc gtcgaatgcg aggcttttta ctatgcttta tcttcgctcc tggcgttcgg
    15361 atatttgcca gccacgtgat tcgcgttaca cttgcggcct ttagtatcct gccggagttg
    15421 tcatgtcgtt ttcctgtcca ctttgccatc agcctctttc gcgtgaaaaa aacagctata
    15481 tctgtcccca acgacatcag tttgatatgg cgaaagaagg gtatgtcaat ctgctgcccg
    15541 ttcagcataa acggtctcgt gatccgggcg acagcgcgga aatgatgcaa gcacgccgcg
    15601 cattcttaga tgccggacat tatcagccgc tgcgtgatgc aattgtcgcc caactgcggg
    15661 aacggcttga tgaaaaggcc acggcggtgc tggatattgg ctgtggtgaa gggtattaca
    15721 cacacgcatt tgccgatgcg ttgcccgaaa tcaccacgtt tggcctggat gtctcgaagg
    15781 tagcgataaa agcggcggcg aaacgctatc cgcaggtcac tctttgtgtc gcttccagcc
    15841 accgtttgcc gttttccgat accagtatgg acgccataat acgtatttac gcgccgtgta
    15901 aagcagaaga attagcacga gtagtgaagc ccggcggctg ggtcattact gccacgccgg
    15961 gaccgcgaca tttgatggag ctgaaggggc tgatttacaa tgaagtacat cttcatgcac
    16021 ctcatgcaga acaactggaa ggttttacat tacagcagag tgcggagttg tgttatccga
    16081 tgcgtcttcg cggtgatgaa gccgtcgcat tattgcagat gacgccgttt gcctggcgtg
    16141 cgaagccaga agtctggcaa acactggcag caaaagaagt gttcgactgc cagacggact
    16201 ttaatattca cctctggcag cgttcttatt aaccgtggaa gtgcgtccag aggatctgga
    16261 cgccgatgcc gatcagcacc agcccgccga gaatttccgc ttttttccca ataattgagc
    16321 cgataaagcg accaaccatc atccctaatg tcgacataat caaggttgca caaccaatgg
    16381 ccaatgcggt cgcgataatg ttgacctgaa ggaaagcaag accaacaccc acagccatgg
    16441 catccaggct ggtggcaatc gcggtggtta ccagtagcca gaaaccgtgc cgacggcgcg
    16501 gctcttcatc ttcatcatct gagccacgaa aaccctcaat aatcattcgc ccgccgagga
    16561 atatcagcag cacaaacgca atccagtgat tccattcaag gacaaaccgg ctggctaaca
    16621 tgcccattcc ccagccgatc agcggcgtca gggtttcgac ggcaccaaaa ataaggccgg
    16681 ttcgcaatgc ttcagaaaat ttcggtttat ggaggggggc acctttaccg attgatgcag
    16741 caaatgcatc catcgacata ccaaacgcaa gaagaacagt agcagtgata ttcatgacaa
    16801 cgtcctgacc ggggtatcca taaaacacat catcgccccc agcaaacatg catacatgaa
    16861 caacaagccg cgatgggttt tcgcgttcat gcaggcatag cgattgatgt gcatatggtc
    16921 tcgcctgact gaaagtcttc agtccgtacg tgccacgttt tgcaacgagt atgttgacac
    16981 gtacatttcc ggaatctgga aatcggctac tccccaaatg agggcgcaac cttaacatat
    17041 tttgagaata taaaacaact gttggaatat attatttcat tgctttattg ataacgattt
    17101 tcatttatgt ttatatatca atgaaacgtt aaaataaata acaaataagg gaagagataa
    17161 tatagcctaa gctatgtctg attattaaaa atacactaaa catagctttg gctaaattca
    17221 taagttattg agttgatac
//
LOCUS       DADLZM010000085        17183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLZM010000085 DADLZM010000000
VERSION     DADLZM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.085"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(329..673)
                     /locus_tag="JNY73_002632"
     CDS             complement(329..673)
                     /locus_tag="JNY73_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY8048599.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="JNY73_002633"
     CDS             805..2715
                     /locus_tag="JNY73_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY8048600.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="JNY73_002634"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="JNY73_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY8048601.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="JNY73_002635"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="JNY73_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY8048602.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="JNY73_002636"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="JNY73_002636"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY8048603.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="JNY73_002637"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="JNY73_002637"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY8048604.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNY73_002638"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNY73_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY8048605.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8853)
                     /locus_tag="JNY73_002639"
     CDS             complement(8251..8853)
                     /locus_tag="JNY73_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY8048606.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKQSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTNEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9210..9761)
                     /locus_tag="JNY73_002640"
     CDS             complement(9210..9761)
                     /locus_tag="JNY73_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8048607.1"
                     /translation="MRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDR
                     LAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKEL
                     HSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSK
                     YGIYEQEGLVAPSQEPQDVKKAV"
     gene            9987..10214
                     /locus_tag="JNY73_002641"
     CDS             9987..10214
                     /locus_tag="JNY73_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8048608.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
     gene            10208..10639
                     /locus_tag="JNY73_002642"
     CDS             10208..10639
                     /locus_tag="JNY73_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048609.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKVLEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNY73_002643"
     CDS             complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNY73_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY8048610.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(10768..10866)
                     /gene="yoaK"
                     /locus_tag="JNY73_002644"
     CDS             complement(10768..10866)
                     /gene="yoaK"
                     /locus_tag="JNY73_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY8048611.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNY73_002645"
     CDS             complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNY73_002645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY8048612.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            12107..12361
                     /gene="yoaF"
                     /locus_tag="JNY73_002646"
     CDS             12107..12361
                     /gene="yoaF"
                     /locus_tag="JNY73_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY8048613.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(12383..12730)
                     /locus_tag="JNY73_002647"
     CDS             complement(12383..12730)
                     /locus_tag="JNY73_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY8048614.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNY73_002648"
                     /pseudo
     CDS             complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNY73_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            14063..14884
                     /locus_tag="JNY73_002649"
     CDS             14063..14884
                     /locus_tag="JNY73_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8048615.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(14841..15287)
                     /locus_tag="JNY73_002650"
     CDS             complement(14841..15287)
                     /locus_tag="JNY73_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY8048616.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            15457..15561
                     /locus_tag="JNY73_002651"
     CDS             15457..15561
                     /locus_tag="JNY73_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048617.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNY73_002652"
     CDS             complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNY73_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY8048618.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNTGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(16108..17166)
                     /locus_tag="JNY73_002653"
     CDS             complement(16108..17166)
                     /locus_tag="JNY73_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8048619.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQHVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
BASE COUNT         4406 a         4175 c         4358 g         4244 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtaacagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg ggcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgattagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgcctggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 ctgtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat
     9721 tctccattca ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg ggcataaaaa
     9781 tgcgctctgc atttttgctt tctttttcac ctgtcatgct caatctctct gttatataaa
     9841 gaattttaca gaattctttg atttgcattg gttaaaataa aaatgtgcat agctcttgaa
     9901 atgcgcattt ttctgcgata tcgtttttcc cgatagcggt agccggtatc agttaccgaa
     9961 aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc
    10021 agataaaggc tgctgttcgt atgaagggga agacactatc tcaattatcc agagaggcag
    10081 gcttaaagcc tgacactatg cgtaacgtat ttcgctgcca tataccacga tacgaagcaa
    10141 tcattgctag atatttggat atggaaccag cccagatttg gccttctcgg tatatggaga
    10201 aaacatcatg ttaacccatt gggtaacagc gcaagaatgc gtaggaatgt catgctttcc
    10261 taaacatgag gtgagtattc gccgcaatct tgaaaagctt gcagccaatc atccagaact
    10321 acgtcgcaag cgtacgggtt caaaggtttt ggaatttcat atatcagttc taccagcggc
    10381 agcacgtgtt gagttgttat taaaatctga gcaaatagaa acaagccagg ggtattttga
    10441 aatcgcccgc cccacgctgg aagcgcacga ttacgatcgt gaggcactgt ggagcaaatg
    10501 ggataaagcc agcgattccc agcgcagact tgctgaaaaa tggttgcctg cggttcagac
    10561 tgcagacgaa atgcttcagc cgttattggt gcgattgtcg tgctatttat ctacaggaag
    10621 attaaaagtt aacgcttaaa ttgcacaaag gctgcacaca ggcagccttt gctatttttt
    10681 agaggtgacg ttaccaccat cccagctcag tgccgagtac gacaataatc gccacaccca
    10741 tcataataat cgttgttttt ttcatcttta tgctcccggg gcagcatagc cgccaataaa
    10801 aaaccatgct aaaaatacga ccgctgtaat gaagattaca accggaaaaa taataccgat
    10861 tcgcatgtta tcaccaatca aaaggatgtg aatacgcctc aggaatgtag cgctggatgc
    10921 gcgacaaaag gtgttttctg tttgtgtttt ttctcctgat acatcgcaat atcggcagca
    10981 tgcagggcac tgtctgcatc ggttgtttca ggatcgactt ctactatgcc aagactggca
    11041 ccgggataat acaaatcaac atcacctaag tgatattctc cacgtatttg ctgttgaata
    11101 cgttctcgca gcgacgaaat atccgcattc tcgttgttta gtgaaacaac caaaaactca
    11161 tcaccaccga gacgaccaat aacttcgcca ttttgctgga gcgtattaag gcgctcgcca
    11221 acctgaatga gaaacagatc gccactatta tgaccaaaac gatcattgat taatttgaag
    11281 ttatccagat cgataaacgc gatcattatc ttatggttaa gatgccgggc gagggaaaac
    11341 agcgtcgtca gattttcaaa aatcgcccgc cgattcggta gcccggttaa cgagtcggta
    11401 tacgattgcg caatcagcgc agcattggct tcgtgcagct gttcaaccag tgcctctttt
    11461 tgaatatatt gtgcaatcag tccggcgaat aactgtaaaa cctgttccgc gcgttcactc
    11521 cactggcgct tctcactgct ggcggcgcaa agcgtgccat agaatgatcc atccggtaag
    11581 tgaattggcg tgctcagaaa tgtggtgatg ccaagattgc gtgccgcaat acagtcgccc
    11641 caacggtcag ggacttcatc gctgaaaaag cagttttcat caatggcgcg tttgcataac
    11701 gagtaatccc acgagacggt aaaattctcc gggatgtgca ttttctggct gttacgggca
    11761 aacataatat gctgcaggcg cgcttcgaca tccactttgg tcaggtaggt tgattccata
    11821 tcagtgacca tttccagcat ctccagaagc tgtcggacca gactttccag tgactgttct
    11881 ttggcaaggg attgcgagac gcgggcgata atctgatctg acacggaaca actccaggat
    11941 acggcgggta agtgctgatt atttaatctt tgcaggcatg ataaaacatc atcaacaaag
    12001 atgaaacaag acgtaatatg ttttgagtga atgtaataaa aatagtgctg caaaacaaaa
    12061 cgataccgcg atactctaag cttggataaa tatgtgagat caataaatga aaattatcag
    12121 ttttgttctg ccttgcttgc tggtcctggc tggttgttca accccttctc agccagaagc
    12181 acctaaaccg ccgcagattg gtatggcaaa tccggcgtcg gtctattgcc agcagaaggg
    12241 cgggacgctc attccagtgc agacagcgca aggggtcagc aacaattgca aattaccggg
    12301 cggtgaaacg attgatgaat gggcattgtg gcgacgggac catccggctg gtgaaaaata
    12361 accgccggat gccgtactaa aatcacaagc tacgtagcca gtcggccagc accagcgcat
    12421 ggttctgcgt ggtgtttttt gctgcgtaga gcagggtcag cggctgtttt ttggcgatgt
    12481 ccgccagccg ctttccttct tgctcgtgtt gcgccagttc tgcgcgatat tgctcgcgaa
    12541 aggttgcaaa atcgacgact tcgccgtgaa aggctttgcg cagttccgtt gacggcgtga
    12601 tttctttatc ccactcatca agggctaaat cggttttttt gataccgcgc ggccagaggc
    12661 ggtcgaccag tacgcgataa ccatcgctct gttcagccgg atcataaacg cgtttgcact
    12721 gaatattcat cgtgtttgtt ctccctttca cgcaagattt gtcacgtcac tatagcaccg
    12781 gatattagcg aatttctttg tctctcccgg cgcaaagtcc gaaaatcgcc atcagtagtg
    12841 aaagtattgc aacacccatc agtggtacgg accagttacc gttagcatcg tgtattttac
    12901 ccattagcgg cggcccacag gctgccagca aatacccgac ggattgtgcc atccccgaga
    12961 gtgccgccgc ctgatgcgca gaacttgccc gaagaccaat gaacgtcaac cccagtatca
    13021 ttgttgcgcc ggaaccaaaa ccgaaaagca gagtccaggt gatcgcgtgc gctggcataa
    13081 agcagagccc aaccgcgccc actgcgcaca tcaaggcaac gaacgctgca ataccacgct
    13141 ggtctttcac atgatgtaag aaaagtggga tcagcaaacc gggagctgct gtggctagtt
    13201 gcagcaaacc atgcagtgaa cccgcctgtg cttcgctata gccgtgactg atgaggatcg
    13261 ccggaagcca gccaataatc acgtaataga ccagtgagtt gatcccaaga aacaatgtta
    13321 cctgctaggc aagtggcgaa cgccagatac cccgagtatg taaggcgcgt gaggtactca
    13381 aatttgcatg ttgctgactt cgccactgtg gcagccataa aaaaagagcc agcagaggaa
    13441 aacacatcag catgaacaac gcgccttgcc agccaaaacc gttcaaagcc agcggcacga
    13501 ccatagccga tcccagtgcc gctgcagctc ccattgtcag ggaatatgcg ccggtaagtc
    13561 tggcgacgga atgagggaaa tcgcgtttaa ttaatcctgg cagtaagaca ttgcctaatg
    13621 caatcccacc gccaatgacc gctgtaccgc caaataataa gtaaggcgaa gggagagaac
    13681 gaattgcgat acctgcacag atcaaaagta acgcggcaaa caggctacgt tccataccaa
    13741 aacgtcgcgc caccggggca gccagaggtg agattagcgc aaaggccaat aatggcaggg
    13801 tggttaataa gccggtttgc gctgtcgtca gcgagtaagc ggaacgaatc gtatccagta
    13861 acggcgctgc gccggtaaag gtgacgcgta atgttgtggc aatcatcaga atgccagcga
    13921 taaggacaat cctgtttttg ccgcttaatg aagttgaaca ggtcatatgc ttctcattgt
    13981 tacaaacgag gctgtacctt accgttttta ttgttatgtt gaatcaagct aaaatgacaa
    14041 attatcgcta agttcggaca aaatgatgca caggttgaat ctcaatggct atgaacccga
    14101 ccgacaccat gaagccgcgg tcgcattttg tattcacgcc ggaacggatg agttaaccag
    14161 tcctgtacat cagcaccgca aaggacagct gatactagca ttacatggtg caattacctg
    14221 tacggtggaa aatgctttgt ggatggtgcc gccacaatat gcggtttgga taccgggcgg
    14281 ggtgaagcat agcaatcaag tgacagctaa cgctgaactc tgttttctgt ttatcgaacc
    14341 cagcgcagtg acaatgccga caacatgttg cacattgaaa atatcgccct tatgccggga
    14401 attaatttta accctggcaa acaggacgac gactcagcgt gctgagccga tgacccgcag
    14461 attaattcag gtcctgtttg atgaactccc tcagcagcca caacaacagc tgcacctgcc
    14521 cgtttcttct catcctaaaa tccgcacgat ggtggagatg atggcgaaag ggcccgtcga
    14581 gtgggggtca ttggggcaat gggctggctt ttttgcgatg agtgaacgca atcttgcccg
    14641 actgatagtg aaagagacgg ggttgagttt tcgccagtgg cgtcagcagt tacagctgat
    14701 tatggcgctt cagggattag tgaaaggcga tactgtacag aaagttgctc atacgctggg
    14761 gtatgattca acaacggcct ttattactat gtttaagaaa ggcctagggc aaacgccagg
    14821 ccgatatatt gcgggattaa ctactgtttc cccacaatca gcgaaaccag acccgcggca
    14881 ataagcgggc caaccggtac gccgcgaaac agcgctacgc ctaaaacagt gcccaccagc
    14941 agcccagcaa ccagttgcgg ctggctgccc attaacgtca cgccgcgccc acccagccag
    15001 gagacaataa cgcctactgc aattgccacc agcgatttcc agttcagaaa cgagtgaatc
    15061 aaggttgagg gcggtagcgt cccgctggcg atgggggcca taacgccaat cgtcagaatg
    15121 attatcccga tacttagccc ctgtttttca atccacggga aaaaggtgct taacggtgtg
    15181 acgcggacaa tgatcaacac cagaattgaa acggcgacag tggtgttatg actgataaag
    15241 cccagcgcag ctaacccgag caggatcagc aaagtgacat caaacatcgt ggtttccttg
    15301 ccaaaaaata ataagcctgc ttactttacg cttgctgcag gaaggaagta gaaaatcttt
    15361 acgttttttg cataaaaaat caggcgaact gtcatcaaac tgccatttgt gcacgctaac
    15421 aataacgtga aaacagctat ctcccggggg catgctatga acgatcaaat gttcgtcgag
    15481 acactgatta tcacatcatc gttttttgct attgcggttg tgctggtttt gtccgttctg
    15541 cttattgaac ggataggcta gctacgcagt gcggcggaaa ttgaccagtt ctggttgcgc
    15601 aatgcgcaaa taatccgcgg ttttcaaaat aacggattta tccagcatcc ctgtattgaa
    15661 ggcgatttca tcaaatcgtt cgaagagtag agggtcggca accagtttga gttttggatg
    15721 gaagctgaaa ggggggatcg cgccgaagac acagcctgtg agttcatcaa cttccgccgg
    15781 gctggccaac gaagcgcgta atccgcctat atggctggcg agttggctca gatcggcctg
    15841 ttgatcggct gcgaggatcg ccagaacatg ttgatttacg ccattgcctt tgactttaca
    15901 taccagtgcc ttagcaccct ggcctagagc ggtgccacga atttctgaca ccgcttcaca
    15961 tttacctacc gcttcatggg taacgacgcg gaagttagca ccttcttgtg ataataaagc
    16021 gattaactgc tgatgcgtca cgcttccttt agccatttca gtcatttcac gctcctgaga
    16081 ttacaagcaa acaaccacag aaggttatca tgaacggctg tttttgttct gcttattgac
    16141 atataaacgc tcatcggaag ctttatacgc atcatgtaac gtaatcgcta aatccccctc
    16201 ctgatgaccg aggttgtcat ttatttgctt taacttgtcc atgtcaattg caataaacat
    16261 caccgaagaa ccggattgca ccagtttctg caaccgctgc tccagttcag gggttaaaat
    16321 tttgcgatta tacagtccag tcatcgcatc actaatattt tctcgcgaca cattttggta
    16381 taaacggaag tgcatccgga ccatattcag caaaattaac gccgtcaaaa tccagaacag
    16441 aacgcttttc cacgaagacg tgataaagta aagaatatca atggataacg agacacgaat
    16501 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
    16561 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
    16621 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
    16681 cacaatccct tttagctgcc ctgcgacata taccggcgtc agcaaactca tcacgtgctg
    16741 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
    16801 aatatctttg cgatcgatgc caatacttcc tgactgcagg aaggaccagt tattcatcgc
    16861 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
    16921 atagtgaaat ttctcttcgg ctatcccaag acctgaaccg taatcatcct ggtggcggtt
    16981 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
    17041 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatggg cagtcaggtt
    17101 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
    17161 cgacacttcc gactgctcag cag
//
LOCUS       DADLZM010000086        17121 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLZM010000086 DADLZM010000000
VERSION     DADLZM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.086"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(467..1612)
                     /locus_tag="JNY73_002654"
     CDS             complement(467..1612)
                     /locus_tag="JNY73_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048620.1"
                     /translation="MCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSA
                     KDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPK
                     NNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAH
                     SHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQS
                     DISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGC
                     KSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLF
                     DIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(1770..2108)
                     /locus_tag="JNY73_002655"
     CDS             complement(1770..2108)
                     /locus_tag="JNY73_002655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048621.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(2698..3639)
                     /gene="fabY"
                     /locus_tag="JNY73_002656"
                     /gene_synonym="yiiD"
     CDS             complement(2698..3639)
                     /gene="fabY"
                     /locus_tag="JNY73_002656"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY8048622.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3684..4121)
                     /gene="dtd"
                     /locus_tag="JNY73_002657"
     CDS             complement(3684..4121)
                     /gene="dtd"
                     /locus_tag="JNY73_002657"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY8048623.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4118..4990)
                     /locus_tag="JNY73_002658"
     CDS             complement(4118..4990)
                     /locus_tag="JNY73_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY8048624.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4984..5583)
                     /gene="yihX"
                     /locus_tag="JNY73_002659"
     CDS             complement(4984..5583)
                     /gene="yihX"
                     /locus_tag="JNY73_002659"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY8048625.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5682..6467)
                     /locus_tag="JNY73_002660"
     CDS             complement(5682..6467)
                     /locus_tag="JNY73_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8048626.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6501..7397)
                     /gene="yihV"
                     /locus_tag="JNY73_002661"
     CDS             complement(6501..7397)
                     /gene="yihV"
                     /locus_tag="JNY73_002661"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY8048627.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7565..8461
                     /gene="yihU"
                     /locus_tag="JNY73_002662"
     CDS             7565..8461
                     /gene="yihU"
                     /locus_tag="JNY73_002662"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY8048628.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8485..9363
                     /gene="yihT"
                     /locus_tag="JNY73_002663"
     CDS             8485..9363
                     /gene="yihT"
                     /locus_tag="JNY73_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY8048629.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQPGDIVDEMMAKRR"
     gene            9380..10621
                     /gene="yihS"
                     /locus_tag="JNY73_002664"
     CDS             9380..10621
                     /gene="yihS"
                     /locus_tag="JNY73_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY8048630.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLFDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10735..11661
                     /locus_tag="JNY73_002665"
     CDS             10735..11661
                     /locus_tag="JNY73_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAY8048631.1"
                     /translation="MSLIKVPCMQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTF
                     QGCHLVIPHKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGL
                     LAWRDWQISELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTV
                     AAPYGVGIHPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKK
                     IAATKIDHTFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMS
                     CPPNAFNSGIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11860..13896
                     /gene="yihQ"
                     /locus_tag="JNY73_002666"
                     /pseudo
     CDS             11860..13896
                     /gene="yihQ"
                     /locus_tag="JNY73_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            13942..15327
                     /locus_tag="JNY73_002667"
     CDS             13942..15327
                     /locus_tag="JNY73_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048632.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTLYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15370..16773
                     /locus_tag="JNY73_002668"
     CDS             15370..16773
                     /locus_tag="JNY73_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048633.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            16841..>17121
                     /locus_tag="JNY73_002669"
     CDS             16841..>17121
                     /locus_tag="JNY73_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048634.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         4267 a         4338 c         4116 g         4400 t
ORIGIN      
        1 gggatattta tttgcataat ctgaatggtg tagccgaaaa acgtcagcat cctaacagca
       61 ccaggattta aggtgaaatt aatctttcat cacagaccgg tcagtactgc caccaaccgg
      121 tctttacttg ctcatcgtta taattttgtt ggtaacagtt cgggaaacaa ggaattcctt
      181 ccggaacacc cccggcatca tccgatagca ttgacgtcaa tcatcagcgc gacgatgagt
      241 ttttttaatc gcatcatagg gtggcgacgt tcagcacgat tacgggtcat agcaaaactc
      301 ctgagcaggc ggccactatg ccgctttcac tggagttctg aatactggta ctttctgtat
      361 ggcttcgcca tgcccctcgc ggctaagtac ctgcacacat ctctctttgt atgtcatttt
      421 tgcaatgcat tgtccgaatt ctgataatcc taatgagttt tactcgtcag actcccatcc
      481 ctacggtata cgagttatat tcatgaatga aatcgacatt tttaaaaaac gaatcttgta
      541 aaacatcagt gttgatatta ccttgcaatc caacattgaa tttgttagag acatgaagaa
      601 ctcgaatatc aaaaaggttt tttaacatca ttaattcttc gggtgttaat gaatcgcaac
      661 taaaatatcc ctgttgatca aaaagatttt ctatttctcc actatcggcg aggcgttttt
      721 caaggagtgt ttgcaacatc gagttgccaa ggacaatgga tatcgtgcga tctttcgcag
      781 atttacaccc catcacaacg gcaacattcg tatccccctc aagagtttca tttatcgccg
      841 tcgctaaaat ggtggtcaaa cacgcaaacg gaactgtatt tctttcgtga tttaatttat
      901 ctaccagttc attccattta tcaaaagccg ggctatattt gttgcgcaca gaaatatccg
      961 actggttaaa caattcagcc atgcgatttt ttatcatctc gatatccggc gaaccagaaa
     1021 gactttgcgc ataatacggt cctgatgatg ttgcatacat taatgccaag cccacacctg
     1081 tcgccatgat gggcgcagcc acggttttgg caagggcgac gctgcgatgg ctatgcgctt
     1141 cacaggctaa aggaacatat tccactgact gcggtaattc cttacatgcc tccctcatca
     1201 ttgcggtatg ctttacaaac aacggttttt cactgcccaa aaaaactttc tgcttaattg
     1261 cactaagaaa acgcaaatca aaaataattg tttttggttt attatttttt ggcacatttc
     1321 ctattatccc gctaagaacc ttccttgctg catggagatt tttttgcaca gcacataaca
     1381 caattgatga agccttctcc actgcattat tgattttaat tttaaaatcc gcatgatata
     1441 cgttgacggg caatgcaagt atatctggac ctacatcttt tgctgagtat ttattggtga
     1501 tcgacggacg tgaacccacg agaaaactta gctgcaaatt atgctctata ttatcagctg
     1561 tttttgtggt gtggttaaac atagagatgc cacctttcat tctttcacac atatcctcaa
     1621 ctttttcact tcgttcttgt ggcaatttgg ctttaacatt aatttgatga tttttacccg
     1681 tgcattcctt aactacaagg ggaatgttat ctaaagtgcc atacaggcta ataacgtcac
     1741 caggattttt atcatgtaag taagtttgat taatacttga agcattcaca aagagggtca
     1801 atattataac tgtttataac ttcacctaca tcaagtgtgt agtgcttact ctggagactg
     1861 ccatccttat ttaattgtat atcaatcaat atatccccgg actttgtcac catatctttg
     1921 ccataaacca tttgcatagc catttcgagg agatttttat ctaactgatt gcgggcgttg
     1981 agtaagcgat taccaaataa tttatatact gttcccgtca ttgccagcgc tgcgctttct
     2041 ggtactttgc gtttccacag tgaggtttct ctatttttaa acaggtcata ttcattatta
     2101 ctcgacaaac catgcttcag ttcgttaaca caatcgcaca gttgctcctc accttccatt
     2161 ttaaggcaaa gtgaaaattg atttaatggc gtttacttcg gtgatttatt actcattagt
     2221 tgtttttcta aattaacaac agaataactt tcttgtttaa tgagattaat attggcaacc
     2281 attatagcct ccttcccacg gtttaagagc aaacatacaa cataatgatt cataacatac
     2341 agagcagtaa atgaatgata ctgacaatgt atgccagccc attatctttg cataatgttt
     2401 gtgagctata gattttaaaa atatttatga aaaaagtatg gcaccagagg gggttttgac
     2461 tatcaaaaat cgctcaaacc tgaactaaga tgatgagtta agcattatct gcaataattg
     2521 ccactcagct ctacaaataa tatttaaatg ctcctgaaca gtgatgtagc aagacacgta
     2581 tggtgccaac gaagaataaa aatttataaa gttcttattc aatataaccg gaatagtcag
     2641 ggactgatgt aaatgaccgg aatgaatgag agaggcagca ccgcccctct ccataagcta
     2701 ctcttcttcg ttcccgccct cttcatacgg gccaaatggc ttcgcgggca gaacgatata
     2761 cgtgccttca aacactgcac ccggcgtctc gtcgccaaag atttcgacct gcatctgcac
     2821 ccgtgctttt cgtccgcgcg ccagacggtc gagatcgccg cttaaggcac caaggtcggc
     2881 taccgcatgg ggtttaccgc taatcggttt gctgtagcgg atatgcgcat ccgccagaat
     2941 aatcgttccg ccgaggtggc gttcacgcag catcagccag ataagccccc aaccggtgag
     3001 cgtcgccagc gagaataaac tcccggcaaa cagcgtatgg tgcggattct gattgccggt
     3061 ttccggcatg gtggtgataa atttttgccc ggtatattgt tgaatacgca cgcccatttt
     3121 ttcactaagc gggatatgtt cgtaccacgc ctgttgcagc tgcgcgcacc agtcgccgcg
     3181 atgcaaaata tcatccagag tggcgacggg cttaatcatc aaaaaatggc gaatcggcgt
     3241 ggtggttggc gttgtgattt ctccctgatt aacaaacccc agcttggcga aaaactccac
     3301 cgcgtcttca cgggcgctac aggtcacgcg cttaacgcct tcctgacgcg ccaccgactc
     3361 cagggtcatc gccatcagcg ttcctaaccc tttgtcctgc acgtccgggt gaacggccat
     3421 aaagcgaatg gacgcttcat tgtcggcatt aatatacaat cggcctaccg ccaccagatt
     3481 accctgctcg tcgacgacca tctggtgatg cgccatcgca tcccacgcgt cgcgttccga
     3541 accttttggt tgatgcaggg gcttacgcaa catttcccag cgaaactgat agtaacgctc
     3601 taactcttct tctgtttgtg gaacctgaag gtgatacata gcgatactct ctcttgtttc
     3661 ccgtgacaac cctggaagct ggctcatacc tgcaaccaga atgtcacggg gccatcattg
     3721 accagcgata cctgcatatc ggcagcgaag cgtcctgttt gcgtgttcat ctcttgctgg
     3781 cggcagcgtt cgacgaaata gtcatataac gcctctgcgc gatccggtga tgcacctttg
     3841 gagaaacttg ggcgcatccc ccgttcggta tctgcggcga gggtaaactg ggaaaccacc
     3901 agtacactgc cgcccgcctg ttgcacgttg agattcatct tgccttcggc atcgctaaag
     3961 atgcggtagc cgagcacacg ctcgcacaga cggtttgctt tctgttcgtc gtcatccttt
     4021 tcgacaccca ataacaccaa aagtcccgcg ccaatttcgc ccgtcacttc tccctccacg
     4081 gtgacgctgg cacgggttac gcgttgaatt aatgcaatca tggttcgtcg tcttcttctt
     4141 gttcagctgc ttgttttagt ttgcggtatt ccccgagagt gacagtaatt tccgcgccaa
     4201 gcaagacgat acaccatgtc cagtagaccc aaacaaagag aatggggatc accgccagca
     4261 caccgtaaat gagctgatat gacgggaaca tggtgatata aagcgcgaaa cctttctttc
     4321 ctgcttcgaa cagaagtgcg gcgacaaacg cgccgacaat cgcgtcgcgg ttaggtacgc
     4381 ggatggtagg aacaatgctg tacagcaacc agaaggagat ccacgacaac agcaacggaa
     4441 aaatacgcag cacgttgtcg atgacagtat tgagatcgct cgcccagcgc agggagagca
     4501 aataggaact gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt aaaatcatcc
     4561 agtacacggc gaacgagtaa atttttggtc gcgctcgttt actgcgccag atggtattca
     4621 acgcgctatc gatggagtac atcaacaata acgcggtgac gatcagcccg cacgccccaa
     4681 cggcggtcat cttgttggaa ttagcaacaa attgttcgat ataccgctga ataacatcgc
     4741 cagtagcagg cagaaagttg gcaaaaataa agtgacgtaa ctgaatgctg acgtcggaaa
     4801 acatgggaaa agcggcgaaa agcgcaaaaa caacggcaac cagcggcact aatgagagca
     4861 acgacacata ggcaaggtta cctgccaggg ttgtcatgtt gtcctcatca atacgttgcc
     4921 agagtagttt tagccaggcc catagtggac gggtacggtg cctggctttg tcctgaatgg
     4981 tttttagcat aacaccttcg cgaaatagtc cgggatggtg gttttatctt tcaccagaat
     5041 actggtaatg cctagctgat tagctccttc tatattatcg gcgttatcgt cgaaaaagac
     5101 cgtatcgctg ggtgaaaaac cttccgcctg caaaacatgc tggtaaattc gtgcttcagg
     5161 tttgcgcatc cccagatctt gcgacagata gatatggtca gcagcatcac gaatttccgg
     5221 gtattcttcc ggccagaagg tggtatgcag gcggttggta ttggaaagca ccaccacgcg
     5281 atgcccctgc tcacgcagtt tatgcatgat ggcgatcact tccgggcgca gcgcaacaaa
     5341 caccgcctgc cagccgtgag agaactgctc gtagcttagc ggtagagcca tctcatgaca
     5401 cagcgcctct gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat gaaacgcctc
     5461 ccccatatga aaactcttct taagcgatgc cagcggaata cgcgttaaat cgctccaggc
     5521 tcccagcaca cggttaaagt cgatatcgac aatcacatta cctaaatcaa agatatagag
     5581 catttttgcc tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga tgggtttggc
     5641 tatgtggcaa ggtatgaagc caggttaacg gtcacaaaaa tctacgcgtc ttcctgggga
     5701 agaatgattt caatttggct gtcttgtaag cgtgatttta gcgcagcggg cggcagctcg
     5761 tcggtaaaga gcgcagtgac ctgtgccacg ttaccaattt caaccgccgc cgaagcatga
     5821 tacttagtgt gatcggcgac cagcagaata tttctcgcgt gcgccatcat cgttttcacc
     5881 acgttagctt cgtttacatc aaactccatc aacgcgccat cgctctcaat cgccccaacg
     5941 cttgttacca gataatcagc acgaaaatca gccacaaagg acgccgctga agggccaatg
     6001 atcccgctat tatgagagcg caacgtaccg ccgggcacca tcacttcaaa gcgcgggttg
     6061 tggtaaagaa tatgcgccac acgcaggctg ttggtgatta tccgcaaatg attatggtta
     6121 agtaacgccc gggcaacatg ctcaacagtc gtaccaatgg tgataaatat tgttgaacca
     6181 tcaggaatat agtctgccac cgcttcggca atcgcttttt ttttctcggt ttgcgaaacc
     6241 tcacgctgct cgaacgccgt attaacgacg ctggaagccc gacccgcgcc accgtgatgg
     6301 cgcgtaatca ggccttgctc gcttaattta cgaatatccc ggcggaccgt ctgcgtggag
     6361 acatccagca gatttgccag ctcatcaata ttcatatacc cacgctcggc gatcagcatg
     6421 aggagttggt cgtgccgcgg gttaccggtt agttcggtaa ggctcatgaa aattcctcga
     6481 aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa aagatcgggt ttgatcacag
     6541 tcagggatcc cggctcgtcc accgggacgt gtgcatttta acgccgctac accgctggcg
     6601 aagcggactg actccgctaa atccccactt gttgccagcg ccaccgccaa agcgccgtga
     6661 aaaacatcac ccgcacctgt ggtatctacc acatcaactt tgaaggccgg ttgatgctga
     6721 cgcccaccat tttccagcca gtcgcagcct gcgctaccct gggtcacata gacatgacca
     6781 tttgtgagcg tttgtgcctg ttttagcgca ctggccatct ctttcacgcc cgttaagcgc
     6841 gccagacccg gttctgaaaa ggccgcgtga tcgcttaatg ccaccagctc actgatatcc
     6901 tgcggcgtaa tgtccccgtc cagaacggtc atcacacccg cctgacgggc cagggtgaag
     6961 gcttttttag cgccgtcgtg ccagcgtaca tctgccagca caacatccca ctgagagaaa
     7021 tcaatttcct ccaaccactc tgcgtcaggc agcagatccg ggctggggta gttaatgatt
     7081 atccgctcgc ctttggtatc caccatgatg gcggattgcg aagatttcgc ctggttatac
     7141 cgtttggtgt aacgggtgtt aacgccccag gattccagct ctgctagcag gctgttgccc
     7201 gcgtcgtcat cacctacgcg accaataaaa tcgacctgcg cccccagcct tgccgccgca
     7261 accgctgccg tcgccgctgg cccgccacca acttccgtat aatttctcgc cacgtattta
     7321 ccgctctccg tcggtaaccc ttccacgtaa tagatgcgat ccatcacggt tatacctaca
     7381 caagcaacac gaatcatggt cattccttaa acattttggg atgcgttcat tttcatttca
     7441 ccaattgttt aaaaagtgac ggctgtcaat tttttgacga taaattacaa atgcgatcaa
     7501 aaacagacaa gcaaagcatg tcataaatga caaaaagtga catcactgta ttcaggagag
     7561 ggttatggca gcaatcgcgt ttatcggttt aggacaaatg ggttcgccaa tggcgagcaa
     7621 tttattgcag caagggcatc aacttcgcgt cttcgatgtg aacgccgaag ctgtgcggca
     7681 tctggtagac aaaggtgcga ctcccgccgc caacccggtg caggccgcta aagatgccga
     7741 atttatcatt accatgttgc cgaatggcga tctggtgcgc aacgtgttgt tcggtgaaaa
     7801 cggcgtttgc gaaggcttat ctaccgatgc gctggtcatt gatatgtcca ccatccatcc
     7861 gctgcaaacc gataaattga ttgccgatat gcaagccaaa ggcttcagca tgatggatgt
     7921 tccggtaggc cgtacttctg caaatgccat taccggtact ctgttactgc tggctggcgg
     7981 caccgctgaa caagttgaac gtgccacacc gatcctgatg gcgatgggca gtgagttgat
     8041 caacgctggc ggtccgggca tggggatccg cgttaagctc atcaacaact acatgagcat
     8101 cgcgctcaat gcgctttcgg cagaagccgc cgttttgtgc gaagccctga atcttccctt
     8161 cgatgttgcc gtcaaagtga tgagcggtac cgccgccggt aaaggtcact tcaccacttc
     8221 ctggccaaac aaagtcctca gcggagatct ttctcccgcc ttcatgatcg atcttgccca
     8281 taaggatctt ggcatcgccc ttgatgtcgc caaccagctg catgtgccaa tgccgctggg
     8341 ggccgcctca cgggaggttt atagccaggc gcgcgcagcg ggtcgcggtc gccaggactg
     8401 gtccgccatt ctggaacagg tccgtgtcag tgccgggatg actgccaaag taaaaatgta
     8461 acgactggat aaaggaataa atgaatgaat aagtacacca tcaacgacat tacgcgcgca
     8521 tcgggcggtt ttgccatgct ggcggtcgat cagcgcgaag ccatgcgcat gatgtttgcc
     8581 gcggctgggg cacccgctcc ggtagccgat agcgttttaa ctgatttcaa agttaacgct
     8641 gcaaaggccc tctcgcctta tgcctcggcg attctggtag atcaacaatt ctgctaccgc
     8701 caggtggttg agcaaaacgc gattgccaaa agttgcgcca tgattgtcgc cgccgatgag
     8761 ttcattcctg gcaacggtat tccggtcgat agcgtggtta ttgaccgcaa aatcaatccg
     8821 ctacagatca aacaagacgg tggcaaagcc ttaaaactgc tggtgctgtg gcgtagtgat
     8881 gaagatgcgc agcaacgtct ggatatggtg aaagagttca acggactgtg ccactcacac
     8941 ggtctggtaa gcatcattga gccagtcgtc cgcccaccgc gtcgtggcga taaattcgat
     9001 cgcgaacaag cggtcatcga tgccgccaaa gagctgggcg acagtggcgc tgacctctac
     9061 aaagttgaaa tgccgcttta tggcaaaggt ccgcaacaag agcttctctg tgcttcacaa
     9121 cgtctgaatg accatatcaa tatgccatgg gtgatcctct cttccagtgt cgacgaaaaa
     9181 ctgttcccgc gtgccgtacg cgtggcaatg acggcgggcg catcgggatt cctggcaggc
     9241 cgtgcagtct gggcatcggt cgtcggttta ccagacaacg agctgatgct ccgtgacgtt
     9301 tgcgcaccga aattacaaca acctggcgat atcgtcgacg aaatgatggc taaacgccgc
     9361 taagatgagg ataaaaggaa tgaaatggtt taacacccta agccacaacc gttggctgga
     9421 acaggaaacc gaccgcatct ttgattttgg taaaaattcc gtagtgccga ctggttttgg
     9481 ctggttaggc aataaagggc aaatcaaaga agagatgggc acccatctgt ggatcacagc
     9541 tcgtatgttg cacgtttatt ccgttgctgc ggcgatgggt cgacctggcg cttacgcgtt
     9601 ggttgatcac ggtattaaag ccatgaacgg cgcactgcgt gataaaaaat atggcggctg
     9661 gtatgcctgc gtgaatgacg agggcgtggt ggatgcctcc aaacagggct atcaacactt
     9721 ctttgctctg ctgggtgccg ccagcgccgt cacaacgggt cacccggaag cgcgcaagct
     9781 gttcgattac accattgaga ttatcgagaa atatttctgg agcgaagaag agcagatgtg
     9841 cctggaatcc tgggacgaag ccttcagcaa aaccgaagag taccgcggcg gcaatgccaa
     9901 tatgcacgcg gtggaagctt tcttgattgt ttatgacgtc actcacgaca aaaaatggct
     9961 ggatcgcgcg attcgcgtgg cttccgtgat tatccacgac gtcgccagaa ataatcatta
    10021 tcgcgttaac gaacacttcg atacccagtg ggatccgctg ccggattaca acaaagataa
    10081 cccggcgcat cgcttccgcg cgttcggagg tacaccaggc cactggatcg aatggggccg
    10141 tttaatgctg cacatccatg ctgccctgga agcccgttgc gaacaaccac cagcatggct
    10201 gctagaagat gccaaaggtc tgtttaacgc caccgtgcgc gatgcctggg cacccgatgg
    10261 cgcggacggg attgtttata ccgttgactg ggaaggaaaa ccggtggtcc gcgaacgtgt
    10321 acgttggcct atcgtcgaag caatgggtac ggcctacgcg ctctacaccg tcactggcga
    10381 tcgccagtat gaaacctggt atcaaacatg gtgggagtac tgcattaagt acctgatgga
    10441 ctatgaaaat ggttcctggt ggcaggagct ggatgcggac aataaggtca taaccaaagt
    10501 ctgggacggc aaacaggata tttatcacct gctgcattgc ctggtgatcc cgcgtatccc
    10561 gttagcgccc ggcatggctc cagcggttgc agcgggtctg ctggatatta atgcgaaata
    10621 actgatctga aaatagggtg cgggttttcc cctcacccta accctctccc caaaggggcg
    10681 aggggaccgt ccggtgcagt ttttgggttt cacatcagcc tcacgggagg ctgtatgtcg
    10741 ttaattaagg tgccttgtat gcaaatcacc aatatgcatt gcagtggaca gaccgtttca
    10801 cttgccgcgg gggattatca cgcgaccatt gtgaccgtcg gtgctggtct ggctgaatta
    10861 acctttcagg gttgtcattt agtcatcccg cacaaacccg aagagatgcc gctggcgcat
    10921 ctgggtaaag tgcttattcc gtggccgaac cggatcgcca acggctgtta ccgttatcag
    10981 gggcaggaat atcagttacc cattaatgaa catagttcaa aagccgctat tcacgggctg
    11041 cttgcctggc gagactggca aattagtgaa ttaaccacca ccagcgttac cctgacagct
    11101 tttctgccgc ccagttacgg ctatccgttt atgctggctt cacaagttgt ttattcgctc
    11161 aacgcacaca cgggtctttc cgttgagatc gcaagccaga atatcggcac tgtcgcagcg
    11221 ccttatggcg tgggtattca tccttattta acctgcaact taacatctgt tgatgaatat
    11281 ctgttccagc ttccggcaaa ccaggtttat gccgtcgatg aacacgtaaa tccgacaacg
    11341 cttcaccacg ttgatgagct ggatttaaat ttcacgcagg cgaaaaaaat agccgcgaca
    11401 aaaatcgacc acacctttaa aaccgctaat gatttgtggg aaatgaccat tacccatcca
    11461 cagcaggcgc tttcggtaag cctttgttcc gatcaaccct gggtgcaggt ttatagcggt
    11521 gaaaaattac agcgtcaggg actggcagtt gaacctatga gttgcccgcc taacgccttt
    11581 aattcaggta tcgacttatt attactggaa ccaggaaaac cgcaccgcct gttttttaat
    11641 atttacggac agagaaaata acttgtattg attagcagtc aaaatcattg aagaacattt
    11701 cactcgctaa aggcaacatt atgtatcttc aatttattaa gttaaaaaca gctcagcttc
    11761 tgtaaagttt ttatatatca gttttatgag aatcatttta cataagcctg taataacagg
    11821 aaggcaacaa tctcgttctc aactctatgg agattagtta tggatacgcc acgtccacag
    11881 ttattagatt ttcaatttca tcagaataac gacagtttta ccctacattt tcaacaacgt
    11941 cttattttaa cccatagcaa agataatcct tgtttatgga ttggctcagg tatagcggat
    12001 atcgatatgt tccgcggtaa tttcagcatt aaagataaac tacaggagaa aattgcgctt
    12061 accgacgcca tcgtcagcca gtcaccggat ggttggttaa ttcatttcag ccgtggttct
    12121 gacattagcg ccacgctgaa tatctctgcc gacgatcagg gccgtttatt gctggaacta
    12181 caaaacgaca accttaacca caaccgtatc tggctgcgcc ttgccgctca accagaggac
    12241 catatctacg gctgcggcga acagttttcc tacttcgatc tgcgcggcaa accgttcccg
    12301 ctatggacca gtgaacaagg cgttggtcgc aacaaacaaa cctatgtcac ctggcaggcc
    12361 gactgcaaag aaaatgcggg cggcgactat tactggactt tcttcccaca gcctacgttt
    12421 gtcagcacgc agaagtatta ctgccatgtt gataacagtt gctacatgaa cttcgacttt
    12481 agcgccccgg aataccatga actggcgctg tgggaagaca aagctacgct acgttttgaa
    12541 tgtgctgaca catacatctc cctgctggaa aaattaactg ccctgctggg acgccagcca
    12601 gaactgcccg actggattta tgacggggta acgctcggca ttcagggcgg gacggaagtg
    12661 tgccagaaga aactggacac catgcgtaac gcgggcgtga aggttaacgg catctgggcg
    12721 caggactggt ctgggatccg tataacctct tttggcaagc gcgtgatgtg gaactggaag
    12781 tggaacagcg aaaactaccc gcaactggat tcacgcatta agcagtggaa tcaggagggc
    12841 gtgcagttcc tggcctatat caacccgtat gttgccagcg ataaagatct ctgcgaagaa
    12901 gcggcacaac acggctatct agcaaaagat gcctctggcg gtgactatct ggtggagttt
    12961 ggcgagtttt acggcggcgt tgtcgatctc actaatccag aagcctacgc ctggttcaag
    13021 gaagtgatca aaaagaacat gattgaactc ggctgcggcg gctggatggc tgacttcggc
    13081 gagtatctgc ccaccgacac gtacctgcat aacggcgtca gtgccgaaat tatgcataac
    13141 gcctagcctg cgctgtgggc gaagtgtaac tacgaagccc ttgaagaaac aggcaagctc
    13201 ggcgagatcc tcttctttat gcgcgccggt tctaccggta gccagaaata ctccaccatg
    13261 atgtgggcgg gcgaccagaa cgtggactgg agtctcgacg atggcctggc gtccgtagtc
    13321 ccgacggcgc tgtcgctggc aatgaccgga catggtctgc atcacagcga cattggcggt
    13381 tacaccaccc tgtttgagat gaagcgcagc aaagagctgc tgctgcgctg gtgcgatttc
    13441 agcgccttca cgccgatgat gcgcacccac gaaggtaacc gtcctggcga caactggcag
    13501 tttgacggcg atgcggaaac tatcgcccat tttgcccgta tgactaccgt tttcaccacc
    13561 ctgaaacctt atctgaaaga ggccgtcgcg ctgaatgcga agtccggtct gccggtcatg
    13621 cgcccgctgt tcctgcatta cgaagacgat gcacatacct attccctgaa atatcagtac
    13681 ctgttaggcc gcgacattct ggtcgctccg gtacacgaag aaggtcgcag cgactggacg
    13741 ctctatctgt cggaggataa ctgggtacat gcctggacgg gtgaagcgtt ccggggcggg
    13801 gaagttaccg ttaatgcgcc catcggcaag ccgccggtct tttatcgcgc cgatagcgaa
    13861 tgggcggcac tgttcgcgtc gttaaacagc atctaatcac atccgcccgt ggggaaactc
    13921 atgggcaaag ggagaacatc gatgagtcac atcacaacgg aagatccagc aactttacgc
    13981 ctgcccttta aagagaaact ctcttacggt attggcgacc tggcctctaa catcctgctg
    14041 gatatcggta cgctttatct tttgaagttt tataccgacg ttctggggct gccaggcacc
    14101 tatggcggca ttatcttttt gatttcaaaa ttctttactg cgtttaccga tatgggtacc
    14161 ggcattatgt tggattcccg acgcaagatc ggtccaaaag gtaagttccg tccttttatt
    14221 ctgtatgcgt cattcccggt caccttactg gcgatcgcca actttgtcgg cacaccgttt
    14281 gatgtcaccg gtaaaacggt gatggccact attctgttta tgctttacgg actgtttttc
    14341 agcatgatga actgctccta cggcgcaatg gtcccggcaa tcaccaaaaa ccccaacgaa
    14401 cgcgcctcac tggcggcatg gcgtcagggc ggcgctacgc tgggcctgct gctgtgcacg
    14461 gtgggattcg tgccagttat gaatcttatc gaaggtaatc agaaacttgg ctatatcttc
    14521 gccgccacgc tgttttcact gttcggcctg ctgtttatgt ggatctgcta ctcaggcgtg
    14581 aaagagcgtt atgtcgaaac ccaaccaacc aatccggcgc aaaagcctgg cctgttgcaa
    14641 tctttccgcg caattgccgg taaccgccca ctgttcattc tgtgtattgc caacctctgc
    14701 accttagggg cgtttaacgt caagctcgcc attcaggtct attacaccca gtacgtactt
    14761 aacgatccca tcctgttgtc gtatatggga tttttcagca tgggctgtat tttcatcggc
    14821 gtgttcctga tgcctggcgc agtcagacgt tttggtaaga agaaggtcta tatcggcggc
    14881 ctgctgattt gggtgctggg cgatctgctc aactatttct tcggcggcgg ctcggtcagc
    14941 tttgtggcgt tctcctgcct ggcgttcttc ggctcagcgt ttgttaacag cctgaactgg
    15001 gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg ttcggaaggc
    15061 acgctctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc tggtttcttc
    15121 cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc tgaccacacc
    15181 attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt agtcaccatt
    15241 gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat tgtggaagag
    15301 atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg ggcgttataa
    15361 ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg ggaaaaaatc
    15421 gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac tctgtatctc
    15481 ctcaaacttt ataccgatga attagggatg cctgcttact atggcgggat tatctttctg
    15541 gtcgcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact cgactcgcgg
    15601 aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc agttccggcg
    15661 gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt taaaacgacg
    15721 attgccaccg cgctgtttat gatgtttgga ctttcataca gtctgatgaa ctgctcgtat
    15781 ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct cgcggcttac
    15841 cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat tccgttgcag
    15901 tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt ctccattggc
    15961 ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt ggacacagcg
    16021 ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg taatccacca
    16081 ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat caaactggcg
    16141 attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc gtggatggga
    16201 ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac tgtaaaatgt
    16261 tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg tgatatactg
    16321 aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt cgcctttttt
    16381 ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt agattacggt
    16441 gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac cttctttcgt
    16501 aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca aattggttat
    16561 gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct catttttatc
    16621 tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta cacactcaat
    16681 gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa agagatggcg
    16741 acagaaaaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat ttccatcctg
    16801 gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga ttaatgcaat
    16861 aattttatta tcttctctga cctctgcctc ggtatttgct ggcgcatacg tcgaaaacag
    16921 ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg gttataactt
    16981 cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag aagatgaact
    17041 aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa ccgataaatt
    17101 aaccatccag cccggtggct t
//
LOCUS       DADLZM010000087        17085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLZM010000087 DADLZM010000000
VERSION     DADLZM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.087"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(464..1660)
                     /gene="hemY"
                     /locus_tag="JNY73_002670"
     CDS             complement(464..1660)
                     /gene="hemY"
                     /locus_tag="JNY73_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY8048635.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1663..2862)
                     /gene="hemX"
                     /locus_tag="JNY73_002671"
     CDS             complement(1663..2862)
                     /gene="hemX"
                     /locus_tag="JNY73_002671"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY8048636.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(2884..3624)
                     /gene="hemD"
                     /locus_tag="JNY73_002672"
     CDS             complement(2884..3624)
                     /gene="hemD"
                     /locus_tag="JNY73_002672"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY8048637.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3621..4562)
                     /gene="hemC"
                     /locus_tag="JNY73_002673"
     CDS             complement(3621..4562)
                     /gene="hemC"
                     /locus_tag="JNY73_002673"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY8048638.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4949..7495
                     /gene="cyaA"
                     /locus_tag="JNY73_002674"
     CDS             4949..7495
                     /gene="cyaA"
                     /locus_tag="JNY73_002674"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY8048639.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENSFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7535..7855)
                     /gene="cyaY"
                     /locus_tag="JNY73_002675"
     CDS             complement(7535..7855)
                     /gene="cyaY"
                     /locus_tag="JNY73_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY8048640.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7903..9216)
                     /locus_tag="JNY73_002676"
     CDS             complement(7903..9216)
                     /locus_tag="JNY73_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY8048641.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9213..9518)
                     /locus_tag="JNY73_002677"
     CDS             complement(9213..9518)
                     /locus_tag="JNY73_002677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048642.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWRYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEVTK"
     gene            complement(9540..10691)
                     /locus_tag="JNY73_002678"
                     /pseudo
     CDS             complement(9540..10691)
                     /locus_tag="JNY73_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10880..11083
                     /locus_tag="JNY73_002679"
     CDS             10880..11083
                     /locus_tag="JNY73_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8048643.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11120..11944
                     /gene="dapF"
                     /locus_tag="JNY73_002680"
     CDS             11120..11944
                     /gene="dapF"
                     /locus_tag="JNY73_002680"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY8048644.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11941..12648
                     /locus_tag="JNY73_002681"
     CDS             11941..12648
                     /locus_tag="JNY73_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY8048645.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12645..13541
                     /gene="xerC"
                     /locus_tag="JNY73_002682"
     CDS             12645..13541
                     /gene="xerC"
                     /locus_tag="JNY73_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY8048646.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13541..14257
                     /gene="yigB"
                     /locus_tag="JNY73_002683"
     CDS             13541..14257
                     /gene="yigB"
                     /locus_tag="JNY73_002683"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY8048647.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14341..16503
                     /gene="uvrD"
                     /locus_tag="JNY73_002684"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14341..16503
                     /gene="uvrD"
                     /locus_tag="JNY73_002684"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY8048648.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16650..17042)
                     /locus_tag="JNY73_002685"
     CDS             complement(16650..17042)
                     /locus_tag="JNY73_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048649.1"
                     /translation="MRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGPMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3956 a         4428 c         4534 g         4167 t
ORIGIN      
        1 ttgatgctgt ttttagtttt aacggcaatt aatatatgtg ttattaattg aatgaatttt
       61 atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact caggaagtta
      121 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      181 ttcccgattc tctgtggcat aataaacgag tagatgctca ttccatctct tatgttcgcc
      241 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      301 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
      361 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgc
      421 atcagaaagg tcccggaggt gctttcctcc gggtgagaag gaactactgt ggcgggttat
      481 tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca
      541 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
      601 ccgcgcggaa ggcgagcgat gcttcctgcc attcaccgtg cttcatcagt gactgaccca
      661 atgtgctcca caacagcggg cgatcaccga cgtttttgat ttgctggcgt agcacttttt
      721 ccagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg cgatcgtcat
      781 actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg tcacattcaa
      841 taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc cggctttggt
      901 ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga tccatcaggc
      961 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
     1021 ccattgatgg gatgatatcc agcagagaac tccatgcccc tgtgcggata tacgcctgtt
     1081 ccgccagacg taatacttca ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     1141 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     1201 gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt
     1261 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttcag
     1321 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcacct tccgccagtt
     1381 tcagcagcgc ctgttcggtc tgcttacgcg cacggcgacg cttacgtccg acaaaccatc
     1441 cacgggtgtg cgcgccagtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     1501 ctaccatcgc cagaatcaat atgatcgcca ggcccgtgac gctggtttcg atattgtagt
     1561 tgtcggtctg gatcagcaca taaccctgat gaccggcaat catcgggcca accactatcc
     1621 ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc cttgcggcgc
     1681 ggctgccgga gtatcacctt gcggtgcagg tgcaggtgca ggtgcaggtg caggtttagc
     1741 ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct gcatcagttt
     1801 ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat tttgctggct
     1861 taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat cgtagtaagc
     1921 acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct gatgacgcgg
     1981 tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat agatatcctg
     2041 atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa tgaagttgtc
     2101 cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg aactggaaag
     2161 ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca gacgcaggtt
     2221 atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat ccacctgcga
     2281 tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa tcagactcgg
     2341 gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag aggtcgtgac
     2401 gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg cctgagccag
     2461 cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga cttcatccaa
     2521 ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag cttgttgctt
     2581 aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg ccgtcagttg
     2641 gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac cccagccata
     2701 caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa tcaatgcagt
     2761 attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca cggcctccct
     2821 ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt cctgttatga
     2881 gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga ccttaatgtc
     2941 ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca atagtcgaca
     3001 gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt gctgcaacat
     3061 ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca tcgcttcttc
     3121 tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga cctcagcacc
     3181 gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta atatcagcgc
     3241 acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc tgatttcccg
     3301 atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg cggtggtgcg
     3361 tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat gcagctgtga
     3421 ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct cccccagcgc
     3481 tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa tcagcggaaa
     3541 atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc cagcgggaga
     3601 cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc agcgaggatc
     3661 tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg ttcggcatct
     3721 tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc gccgaccagc
     3781 gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg cacctgacat
     3841 ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc agtttcgtgg
     3901 tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca ttcaataccg
     3961 actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc gcgaatgcgg
     4021 gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc gtattcgccg
     4081 ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat aatcagatcc
     4141 gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac gatactgcct
     4201 gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg aggatcttca
     4261 cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac atctttcatt
     4321 gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc ttttacaaat
     4381 aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc gcgcgtcacc
     4441 atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc tttgacatag
     4501 tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac attgtctaac
     4561 atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca gagtgatgtc
     4621 agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg gcagtggatc
     4681 ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg gtgctacact
     4741 tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga tcacgtttta
     4801 gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa cggttacctt
     4861 tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga atcacagtca
     4921 tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc tgaaacagag
     4981 actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg ggcctgcatt
     5041 ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc taatgccggg
     5101 ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg aaactcaacg
     5161 ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc caccgaaagg
     5221 tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag ggcaaagttg
     5281 ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg aagagcgcca
     5341 gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg gtgtggaagt
     5401 cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca gcctgggggg
     5461 cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc gtactgccgt
     5521 gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag aagagcatta
     5581 cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg aatggctgga
     5641 tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc tttggcagct
     5701 ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc tggaagccta
     5761 ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc gtttgcacga
     5821 cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc gtgttactga
     5881 atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct gcttctattt
     5941 aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc gcgcagtgtt
     6001 gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc tcgataaccg
     6061 tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg acgcgatgat
     6121 gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg tctccgccag
     6181 tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag cattgccagg
     6241 taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga atctgacctt
     6301 tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata accgcgcgcc
     6361 aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc tgaataaact
     6421 ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt atattaaagg
     6481 taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt cgcaccattt
     6541 cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg agattcgcca
     6601 tctggcgatt atcgttaacc tggaatatga ccctacagct gcgttccgca atcaggtggt
     6661 gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa attgcctggt
     6721 aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc tgcacttcaa
     6781 cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc atcaggacgc
     6841 cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg gcttaatccg
     6901 tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca gcacccgcca
     6961 ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct tgttcttcga
     7021 acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg gcgctatttc
     7081 gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca aattaccggc
     7141 ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag aaacgcaaga
     7201 cgagaatagc tttaatatct acattctcga cgaaagcaac cgggttgagg tgtatcacca
     7261 ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact cgtcatcgca
     7321 cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt tctatcagat
     7381 tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga ttggcaacct
     7441 gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt cgtgatgagc
     7501 gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact gtttcacccg
     7561 cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg cgatcgcaaa
     7621 tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc agccacacct
     7681 ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag gtaatggtca
     7741 gtacgccgcc gttgatctcg cagtcgatat cgctgtcgcc atccctgtcg tccagacgct
     7801 cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg ttcattgttg
     7861 taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct tcaaagtttg
     7921 cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga tgtctctgtt
     7981 ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg gggagggcgt
     8041 aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag cgaagcgatc
     8101 gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc gtaattggcg
     8161 gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca agtctggcgt
     8221 atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg ttacatacag
     8281 ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag gttcaatacc
     8341 tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac gccagcgaac
     8401 gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt tgcctgataa
     8461 ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca gtgaatgagg
     8521 tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc catttaaata
     8581 aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt ttacactcat
     8641 aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac tgctgctccc
     8701 ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag agttaccttc
     8761 gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt cggtgcgctg
     8821 tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct gaaagctggc
     8881 aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc agagaataac
     8941 gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat cggaaggtat
     9001 ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc tcatctccgg
     9061 aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca taacaccgtg
     9121 atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg tgtgtcccaa
     9181 caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt gcgcggggca
     9241 ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt gtaatttacg
     9301 ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt cggatcagcg
     9361 gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt atcgcgggca
     9421 taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc cggagaacgg
     9481 ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga atggtatggc
     9541 tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa acgtttgttg
     9601 ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc agactctgtt
     9661 tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat gggttgaaac
     9721 tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt acgccagaac
     9781 actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc atcccacgcc
     9841 agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca ggtcaagagt
     9901 gagccgggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag ctgttcgccg
     9961 ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa tggtacggca
    10021 atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt ctgcgccagc
    10081 acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa attagcgtca
    10141 cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac ctgatgcagc
    10201 gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc ttcaaaatag
    10261 agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac atcgacggga
    10321 ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg accatcgcta
    10381 tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc ggttatcaaa
    10441 tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat taataaattg
    10501 ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt ttctttataa
    10561 gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc aggtgcggaa
    10621 agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca aaccacattg
    10681 cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc agcagaacat
    10741 aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga cttcataaag
    10801 agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag aaagcgatga
    10861 actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat tacttactct
    10921 cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg cagataaaaa
    10981 cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc cggataaaaa
    11041 cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag tttctgtacc
    11101 cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca acgattttat
    11161 ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc gtcgcctggc
    11221 ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc cgtatgatcc
    11281 tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg
    11341 taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca ataagcgtga
    11401 tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg atgatctggt
    11461 ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc gcgctaacaa
    11521 agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg gcgtggtgtc
    11581 gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg cggtagaaac
    11641 gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata tcggttttat
    11701 gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg caggagaaac
    11761 ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc aaggtttgct
    11821 ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct ggaaaggtcc
    11881 gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat ttattcatct
    11941 atgaagcaac caggggaaga actgcaggaa acactcacgg agcttgatga ccgggcggtt
    12001 gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc agtagaagcg
    12061 atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca catggcccgc
    12121 gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga gcaggctatc
    12181 gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac ggccgccagc
    12241 agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg cctggctggt
    12301 gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa ccacactcat
    12361 ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg gcaggaacag
    12421 cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga agcgaaagcg
    12481 gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt tgtgctgttt
    12541 accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact tcatgaaatt
    12601 gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc gatttacaca
    12661 ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc ccgataaccc
    12721 tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa aacggcctgc
    12781 aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc agtcgccgta
    12841 aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc ttttttgact
    12901 ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg gcaccgaaag
    12961 cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg ctggatattg
    13021 atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg tacggcgcgg
    13081 gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg gagtccggcg
    13141 aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt cgcaacgctg
    13201 tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa gacgacgcgc
    13261 tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa cgctttgccg
    13321 aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta cgtcactcgt
    13381 tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag ctactgggtc
    13441 atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac cttgcctcgg
    13501 tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt accggccttt
    13561 ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata accgtccggt
    13621 gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg
    13681 cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag cggaacccga
    13741 gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga tgctcgacgc
    13801 cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta actttgccaa
    13861 atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac agctggcgaa
    13921 gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt ttggtttggg
    13981 ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga
    14041 tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct tacatgttgg
    14101 ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg cttgttggat
    14161 cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc cacatctgga
    14221 aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt atatataccc
    14281 agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc ggcggtgcca
    14341 atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc ggtggccgcg
    14401 ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg
    14461 catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat tatggctgtg
    14521 acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact gatgggcacc
    14581 agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg
    14641 caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga agaccagcta
    14701 cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg gccgccgcgc
    14761 caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca tcatattcaa
    14821 agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg
    14881 tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca cgaactgtgg
    14941 cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat cctggtggac
    15001 gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc gggcgacacc
    15061 ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg cggggcgcag
    15121 gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat tcgtctggag
    15181 caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat tgaaaacaat
    15241 aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc tatttccctc
    15301 tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat caaaacctgg
    15361 caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa cgcccagtcg
    15421 cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta cggcgggatg
    15481 cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct gattgccaac
    15541 cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg tattggtgac
    15601 cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact ctggcaggca
    15661 tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc cttacagcgg
    15721 tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct gcatgtacag
    15781 actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga gaagggcgaa
    15841 aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg gcagttcagc
    15901 tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca tgcggcgctg
    15961 gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat gaccatgcac
    16021 tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga gggcatgttc
    16081 ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg tctggcctac
    16141 gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac tcgccgtctg
    16201 tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc ggaagagtgt
    16261 gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca gcggatgggt
    16321 acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg ccacgctaag
    16381 tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg tttgcaggtg
    16441 gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct ggagtcggtg
    16501 taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat tcagattcaa
    16561 taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc atcagcagca
    16621 tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga aatcatggtg
    16681 acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg tgaaatcgtg
    16741 ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca ggcaaaatca
    16801 taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt attaatccca
    16861 acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt aattacaccg
    16921 ttttccatca acattggtcc tgactgcacc gcaaactgaa tatctttact ggctttaaaa
    16981 gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc gccaggacgg
    17041 ataaagaaat tcccttcacc tgaagcgaga tttaacgcta ccttc
//
LOCUS       DADLZM010000088        16902 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLZM010000088 DADLZM010000000
VERSION     DADLZM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.089"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..882)
                     /locus_tag="JNY73_002686"
     CDS             complement(<1..882)
                     /locus_tag="JNY73_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY8048650.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="JNY73_002687"
     CDS             989..1285
                     /locus_tag="JNY73_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8048651.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNY73_002688"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNY73_002688"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY8048652.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNY73_002689"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNY73_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY8048653.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNY73_002690"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNY73_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY8048654.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4297..4380
                     /locus_tag="JNY73_002691"
     CDS             4297..4380
                     /locus_tag="JNY73_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY8048655.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNY73_002692"
     CDS             complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNY73_002692"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY8048656.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5858..6700)
                     /locus_tag="JNY73_002693"
     CDS             complement(5858..6700)
                     /locus_tag="JNY73_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY8048657.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6903..8945
                     /gene="prlC"
                     /locus_tag="JNY73_002694"
     CDS             6903..8945
                     /gene="prlC"
                     /locus_tag="JNY73_002694"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY8048658.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADILAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNY73_002695"
     CDS             8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNY73_002695"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY8048659.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNY73_002696"
                     /pseudo
     CDS             complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNY73_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNY73_002697"
     CDS             complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNY73_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY8048660.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12354..12689
                     /gene="uspB"
                     /locus_tag="JNY73_002698"
     CDS             12354..12689
                     /gene="uspB"
                     /locus_tag="JNY73_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY8048661.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNY73_002699"
     CDS             complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNY73_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY8048662.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14491..15690
                     /locus_tag="JNY73_002700"
     CDS             14491..15690
                     /locus_tag="JNY73_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY8048663.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4301 a         4219 c         4363 g         4019 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattggata
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
     3721 gcgtcggttt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     3781 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     3841 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     3901 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     3961 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     4021 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     4081 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     4141 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4201 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     4261 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4321 cttggggcgt taattgtgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4381 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4441 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4501 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     4561 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     4621 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     4681 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     4741 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     4801 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     4861 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4921 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     4981 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5041 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5101 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     5161 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5221 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5281 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     5341 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     5401 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5461 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     5521 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     5581 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     5641 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     5701 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     5761 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     5821 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     5881 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     5941 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6001 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     6061 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     6121 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6181 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     6241 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     6301 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6361 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     6421 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6481 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     6541 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     6601 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     6661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     6721 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     6781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     6841 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6961 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     7021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7081 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7141 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7201 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     7261 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     7321 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     7381 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7441 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     7501 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     7561 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     7621 gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     7681 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     7741 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     7801 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     7861 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     7921 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     7981 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8041 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8101 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8161 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     8221 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8281 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8341 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8401 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     8461 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     8521 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     8581 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     8641 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     8701 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacatac
     8761 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     8821 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     8881 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8941 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9001 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9061 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     9121 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9181 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9241 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     9301 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     9361 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     9421 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     9481 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     9541 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     9601 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     9661 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     9721 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     9781 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     9841 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9901 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9961 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10021 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
    10081 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagtcgt
    10141 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10201 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10261 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10321 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10381 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10441 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10501 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    10561 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    10621 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    10681 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    10741 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    10801 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    10861 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10921 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
    10981 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11041 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11101 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11161 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    11221 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11281 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11341 attacaacag aaaataacca gctgatcaca ctaatgatgg tgttaaattt tgtattggca
    11401 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    11461 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    11521 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    11581 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    11641 accaccagat ccatatcgta tttcttgatt gcatcgacca gaacctgacc caggtcgccg
    11701 ctgccgctca gggtttcagt gattggatag cctgcattag tggaaagctc ggtcagcgca
    11761 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    11821 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    11881 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    11941 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    12001 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    12061 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    12121 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    12181 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    12241 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    12301 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    12361 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    12421 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    12481 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    12541 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    12601 gtgagcgggt gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca
    12661 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    12721 cgggccagta aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagaa
    12781 ccagtacagc ccgccggaaa gtagtaccgc agccggaagg gtaaacaccc aagccatcag
    12841 aatactggtt acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    12901 agaagaggag agtacgtgag tggtggaaac cggcatcccg gtataactcg ccaggccgat
    12961 tgacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc
    13021 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    13081 cgccagcgcg accgccatga tgatccacac cggcgcatac tcgatggtgc taagcatgtc
    13141 ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acaccaggca tcttcaccac
    13201 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctgactac gttgatcaag
    13261 cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc
    13321 gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg
    13381 agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa
    13441 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    13501 catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat
    13561 gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag
    13621 cgcgatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    13681 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    13741 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    13801 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    13861 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    13921 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    13981 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    14041 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    14101 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    14161 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    14221 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    14281 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    14341 ttttttgatt tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag
    14401 gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt
    14461 cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc
    14521 gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg
    14581 ctgatcgata atggtaaaat actagggcgc aaaatcctta tgtctggcgg tgggcgctgc
    14641 aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt
    14701 aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa taaacacggt
    14761 atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt
    14821 gtcgacatgc tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa
    14881 gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg catgactgtc
    14941 ggttgcgaaa agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcatcg
    15001 ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt
    15061 ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg
    15121 gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc
    15181 acccaccgcg gcttatctgg accggcggtg ttgcagattt caagctactg gcaaccgggt
    15241 gaatttgtca gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag
    15301 cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct accgaagcgg
    15361 ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac
    15421 gtgtgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc
    15481 actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactttct
    15541 tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac
    15601 gttaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt
    15661 gcgcaggatt tggcgtcaat tgtgaaataa ccatgacgtt ttcccgtcag ccgtttgtat
    15721 atgtcgttat taagtcattt tggtacggtt ctgcatccga cattctggtg ttaactcgct
    15781 gttttgttag cgaattaacg ctattgccgt tgtggttatt ttatagtgta tttattctca
    15841 gagaatatat attactgaaa taaaaaacac aaagaataac caaaggaaat tatttgaaac
    15901 gtaaaaggta tatattttta atctgtaaaa tttaaaatca ccccctctgg attgaatcaa
    15961 cttgctctat ttaagacgat tgtttttaca ttttcaccac ctgtatcagg tggtgttttt
    16021 ttattggcgg caatgccttt atcttatttt tattacgttg taaatatggc tattcctaaa
    16081 tgaaatttga tttgctcagt ttatttgtga tgtcagggga gtgcttgcca tgagagtcgt
    16141 gatattatta ttcatcattt ttataccgag tgtggcggat gctttttcat tcgatagttc
    16201 aataattaat ctcaaatgtc ctaggcgtgg aactatcgaa ataatattac acagatacga
    16261 acacacccag gaaagctggg gaggggagaa ttttgaaacc gggagtgggt atagtcatga
    16321 gggcgcgtta tttatttttg actttgccaa tcaggataaa atgatttaca atcgcgcgga
    16381 gaattcgttt gctttttggt atgaatcttc ccggaaatta gttaactgta acttaataac
    16441 gcttaaaagc acatatcctg tcaaccttcc ttatgttcat ggctgatgtg atgtgatgtg
    16501 atgtgtgtcg ggaactaaca tcttcatctt tgtatatgac attgcgataa tgttaagctg
    16561 gtctttgtca taaatttacc cttagagata ctactccctt ctgtcaatat tattcaagcc
    16621 gaacgttata ctggcttttg tcggtcacgg atatcttttt ttcagtgaga attcaaagtg
    16681 agggtatata agaagtcgta tgagtaagta tctttgttgt ggtgggttat tattttatgg
    16741 gcaaaatatt aagcagcgaa atcttttttt tgcccagacg ttttttgtag aaaaaagaat
    16801 ttattagtcc ggctgaaaag caagaactgc tataactaat taagcgtaac ggaacggtac
    16861 aaaacatgcc cttacaacaa ttttcccgat tagcgctcac tc
//
LOCUS       DADLZM010000089        16758 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLZM010000089 DADLZM010000000
VERSION     DADLZM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.090"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            191..2045
                     /gene="ldtD"
                     /locus_tag="JNY73_002701"
                     /pseudo
     CDS             191..2045
                     /gene="ldtD"
                     /locus_tag="JNY73_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            2226..2774
                     /locus_tag="JNY73_002702"
     CDS             2226..2774
                     /locus_tag="JNY73_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY8048664.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2801..3448
                     /gene="gloC"
                     /locus_tag="JNY73_002703"
     CDS             2801..3448
                     /gene="gloC"
                     /locus_tag="JNY73_002703"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY8048665.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3671..4861)
                     /locus_tag="JNY73_002704"
     CDS             complement(3671..4861)
                     /locus_tag="JNY73_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY8048666.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5046..6134)
                     /gene="ompF"
                     /locus_tag="JNY73_002705"
     CDS             complement(5046..6134)
                     /gene="ompF"
                     /locus_tag="JNY73_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY8048667.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6736..8136)
                     /gene="asnS"
                     /locus_tag="JNY73_002706"
     CDS             complement(6736..8136)
                     /gene="asnS"
                     /locus_tag="JNY73_002706"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY8048668.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8305..9507)
                     /gene="pncB"
                     /locus_tag="JNY73_002707"
     CDS             complement(8305..9507)
                     /gene="pncB"
                     /locus_tag="JNY73_002707"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY8048669.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9773..12385
                     /gene="pepN"
                     /locus_tag="JNY73_002708"
     CDS             9773..12385
                     /gene="pepN"
                     /locus_tag="JNY73_002708"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY8048670.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12592..13359)
                     /gene="ssuB"
                     /locus_tag="JNY73_002709"
                     /pseudo
     CDS             complement(12592..13359)
                     /gene="ssuB"
                     /locus_tag="JNY73_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13356..14003)
                     /locus_tag="JNY73_002710"
                     /pseudo
     CDS             complement(13356..14003)
                     /locus_tag="JNY73_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(14015..15160)
                     /gene="ssuD"
                     /locus_tag="JNY73_002711"
     CDS             complement(14015..15160)
                     /gene="ssuD"
                     /locus_tag="JNY73_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY8048671.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15157..16116)
                     /locus_tag="JNY73_002712"
     CDS             complement(15157..16116)
                     /locus_tag="JNY73_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8048672.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16109..16684)
                     /gene="ssuE"
                     /locus_tag="JNY73_002713"
     CDS             complement(16109..16684)
                     /gene="ssuE"
                     /locus_tag="JNY73_002713"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY8048673.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4186 a         4319 c         4289 g         3964 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga tgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cacaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg caggatggaa tgacaaacgt atttctgatg
     1861 ctctgaagca gggtgataca cgttacgtca atattcggca gtcgattccg gtgaatctct
     1921 actacctgac ggcctttgtt ggtgcagatg atcgtaccca gtatcgtaca gatatttaca
     1981 attatgatct gcctgcgcga tccagctcgc aaatcgtatc gaaagcggaa caattaatca
     2041 ggtaaatgaa gtagttcggg gaaatcaatt gtcgtaataa ttagcatgat tgggggcgat
     2101 tctctgcagc ccccgtcact gctggggttg agtcatcttg acgtctgctt tacgggcggt
     2161 taaggtgcct cttgtgcgcc agaagtgcat ataaacgata acattgacct gtagacttga
     2221 ttatcatgga caaattcgac gctaatcgcc gcaaattgct ggcgcttggt ggcgttgcac
     2281 tcggtgccgc catcctgccg acccctgcgt ttgcaacact ctctacccca cgcccgcgca
     2341 ttttgacact caataatctt cataccggag agtcaatcaa agcggagttt ttcgatggca
     2401 gaggctatat tcaggaagaa ttggcaaaac ttaaccattt tttccgcgat taccgcgcga
     2461 acaaaataaa gtccatcgac ccaggattat tcgaccagtt gtatcgcctg caagggttgt
     2521 taggcacgcg caaaccggtg caactcattt ccggttatcg ttccattgat accaacaatg
     2581 aactacgcgc ccgcagccgt ggagtagcga agaaaagcta tcacactaaa ggccaggcga
     2641 tggatttcca tattgaaggt atcgcgttaa gcaatattcg caaagccgcg ttatctatgc
     2701 gcgcaggtgg tgtaggatat tacccacgta gtaactttgt gcatattgat accgggccag
     2761 cacggcactg gtagtaatcg cttaacgaaa caggggcagt atgaactatc gtattattcc
     2821 ggtcaccgca ttctcccaga actgttcatt gatctggtgt gaacaaaccc gcctggccgc
     2881 actggtcgat cctggcggcg atgcggaaaa aatcaaacag gaagttgatg acagcggcct
     2941 gacactgatg cagatcctgc tgacgcatgg tcatctggac cacgttggcg cagcggcgga
     3001 actggcgcaa cattacggcg tgccggtttt tggcccggaa aaagaagatg agttctggct
     3061 gcaaggcttg cctgcgcaaa gtcgtatgtt tggtctggaa gagtgccagt cgctgacgcc
     3121 agatcgttgg ctgaacgaag gcgataccat cagcataggg aatgtgactt tacaggtgtt
     3181 acattgccct gggcatacgc cgggtcatgt cgtgtttttt gatgatcggg caaagctgct
     3241 gatttctggc gatgttattt tcaaaggcgg agtagggcgc agtgacttcc cgcgtggcga
     3301 tcataatcaa ctgatttctt caatcaaaga taaattgctg cctctggggg atgacgtgac
     3361 atttattccg ggtcacggac cattatctac acttggttat gagcgcctgc acaatccctt
     3421 cctgcaagac gaaatgcccg tctggtaaag gcgcataaaa aagtccactt ttaatgcggg
     3481 cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg
     3541 gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag
     3601 cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg
     3661 tttttaacgc ttacagcact gccacaatcg attcgcacag cggagccatg ttatctggtg
     3721 tcatcccggc cacatttacg cgaccagaag caacagcata tacgccaaac tcttcgcgca
     3781 gacgcagcac ttgttctttt gtcagaccac tgaaggagaa catgccgttc tgtttgatga
     3841 taaagctgaa gtcgcggttt gcaccttttt cctgcagcgt attgacgaac aactgacgca
     3901 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaatgcat
     3961 cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggagggtta gagtagttag
     4021 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac gatttcactg tcggcagcaa
     4081 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
     4141 caacaatcag ctctttatgc atagccgaga aagcgcgcag tccttcagca tcttcttcca
     4201 gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg
     4261 agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
     4321 agcagccatg gaacagcact acgtcgccag cctgagcttc gttcagactg ttaatcagtg
     4381 catcgaagtc aagggtgtga ttttccgcat cataataagc gtattcacga acttccagac
     4441 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacacacgct
     4501 taacgctggt attttttgcc aggaagtcgg cagccacgcg tagtgcgcca gtgccacccg
     4561 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
     4621 gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg
     4681 tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
     4741 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
     4801 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
     4861 tgacgaggtt ccattatggt tatagaaggg aagtccgcta tcagggtaac gggagattta
     4921 caaaattcca attattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
     4981 tggaaggcat aaaaaaaaca ggaccaacgt cctgtttttt cggcatttaa caaagaggtg
     5041 tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt
     5101 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
     5161 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
     5221 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
     5281 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat
     5341 ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
     5401 ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt
     5461 tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct
     5521 tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca
     5581 cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag
     5641 ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat
     5701 gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca
     5761 ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc
     5821 agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta
     5881 tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca
     5941 agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa
     6001 aaataatgca gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag
     6061 atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata
     6121 ttgcgcttca tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt
     6181 caataagttc tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt
     6241 gtctgcaggc atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat
     6301 aaagatttag tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggagctttgt
     6361 gaacaccgtc atatttccat agaaacgtga tgatatttac agcaattttt atctatttat
     6421 atgatttact tatatttaaa ttaactaaac ggaaattttg tttctgatgg aaactttatc
     6481 gacctggcac aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt
     6541 gctatcagct aagaaaaaac gaatccgcaa taagaatgcg ggttattttt tgtgcgtaaa
     6601 tgttgcaatg taatattccg taacaggatg atcgttatgg cgggggtgca aatagattgc
     6661 ttgacttaac aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct
     6721 ctttgaggaa gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc
     6781 acgcacgttt tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc
     6841 tgaatgcgga acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt
     6901 caggcccatt tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc
     6961 accaatgatc tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc
     7021 ttcgttgagg cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg
     7081 tgctttaaag tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca
     7141 gtaaaccggg ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta
     7201 atccacctgt gcgaaatcgg cttcaatgaa gcgttccaga cgtgaaacgg catctttatc
     7261 tacgcgttca gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac
     7321 atatttcagc atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc
     7381 cggctccagc atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa
     7441 agtcgggccg aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg
     7501 gccagatacg gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc
     7561 ctgatcgtta cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc
     7621 accttcggta tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt
     7681 aaagaagcga tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag
     7741 gtttgtgcgc ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc
     7801 cgccatcggg taagtgtctg gatcttcaac ccaaccagca acttcaacct tgctggcctg
     7861 aatttcaaat tgttgcccct ggcccggcga tgccacgact ttacccgtca caatgaccga
     7921 gcagccggtg gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac
     7981 agcctgtaca ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt
     8041 tgaatctcgg cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg
     8101 gccctggagt acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg
     8161 aaaaaataaa cacttgtcca cccgtaaatg ggggggatac ctatgttacc tggcatctgc
     8221 aatcagacaa gcagaattcg caaatgcagc gaaagatttc ggaattaaag gtaaaaaagg
     8281 gagccgatta gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc
     8341 tttgcgcagc gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga
     8401 aagtttcgcc accggtttac cgttacactc taccaactta atcacaatat tcaggggttt
     8461 tacctggggg atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga
     8521 agagaagtgg cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag
     8581 cgttttactc tgtggatcaa ttcccagctt ttcataatgt gcaatggctt tttcacccca
     8641 ttcaaccggg tcgccagagt catgacgcag gccctgatac cgactagcga actcgacacc
     8701 gaaatcacgc aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc
     8761 gggatactct tccagccagg cagcaagtgc agctcgctgg ctgttggcta gatccgggct
     8821 gatttgctga tgtgcctgga accattcgtg tgcctgtgtt cccatcggcg tgagggaaag
     8881 ccgacgcgcc agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt
     8941 aacgatggtt tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg
     9001 gaagcgcgac atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag
     9061 cgtgtcgagg gcttgtgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact
     9121 gataaccgcc agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg
     9181 aatatccagc ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaactc
     9241 gcgtaaccag ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc
     9301 atcatcctgc aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata
     9361 aatacccagc agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata
     9421 gtgatgaaac acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg
     9481 cagaacagga gaagcgaatt gtgtcatagg tgcgcagtag cgtcctcgta caggagcgtt
     9541 tagtacaata aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac
     9601 cccgatcaca ccatatgcca cctttctgga cgatggcatt acgcggtgta tgttataaaa
     9661 atgtagcaat aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg
     9721 caacaggaat agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca
     9781 acagccacaa gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat
     9841 tgacttgacc tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt
     9901 ccgtcatggt gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc
     9961 tgttcatatt aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg cactggtcat
    10021 cagtaatttg ccggagcgtt ttacgcttaa gatcattaat gaaataagcc cggcggcgaa
    10081 tacggcgctg gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga
    10141 aggtttccgc catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac
    10201 caaaattatt gccgataaaa tcaaatatcc cttcctgctt tccaatggta accgcgttgc
    10261 gcaaggcgaa ctcgaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc
    10321 gtgctacctg tttgcgctgg tggcaggcga ctttgatgta ctgcgcgata cctttaccac
    10381 gcgttctggt cgcgaagtag cactggagct gtacgtcgat cgcggcaacc ttgatcgcgc
    10441 gccgtgggcg atgacctcgc tgaaaaactc catgaaatgg gatgaagaac gctttggcct
    10501 ggagtatgac ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat
    10561 ggagaataag ggtctgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc
    10621 caccgacaaa gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg
    10681 gaccggtaac cgagtgacct gccgcgactg gttccagctc agcctgaaag aaggtttaac
    10741 cgtcttccgc gatcaggaat tcagctctga ccttggttcc cgcgcagtta accgcatcaa
    10801 taatgtgcgc accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc
    10861 aatccgcccg gacatggtca ttgagatgaa caacttctac accctgaccg tttacgagaa
    10921 gggcgcggaa gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg
    10981 gatgcaactc tatttcgagc gtcatgatgg tagcgcagcg acctgtgacg actttgtgca
    11041 ggcgatggaa gatgcgtcga atgttgatct ctcccatttc cgccgttggt acagccagtc
    11101 cggtacgccg gttgtgaccg tcaaagacga ctacaatccg gaaactgagc agtacaccct
    11161 gaccatcagc cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat
    11221 tccgtttgcc atcgaattgt atgacaacga aggcaaagtg atcccgttgc agaaaggcgg
    11281 tcatccggtg aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa
    11341 tgtctacttc cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga
    11401 atataagtgg agcgatcagc aactgacctt cctgatgcgt catgcgcgta atgatttctc
    11461 ccgttgggat gcggcgcaaa gtttgctggc aacctacatc aagctgaacg tcgcccgtca
    11521 tcagcaaggg cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcggtgct
    11581 gctcgatgag aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa
    11641 tgaaatggct gaactgttcg atatcataga cccgattgct attgccgaag tacgcgaagc
    11701 actcactcgt actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa
    11761 ttaccagagc gagtaccgtg ttgagcatga agatattgca aaacgcactc tgcgtaatgc
    11821 ctgcctgcgc ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca
    11881 ataccatgaa gcaaacaata tgaccgatgc gctggcagcg ctttctgcgg cggttgctgc
    11941 acagctgcct tgccgtgacg cgctgatgca ggagtacgac gataagtggc atcaggacgg
    12001 tctggtgatg gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga
    12061 gacggtgcgt ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg
    12121 ttcgttgatt ggtgcgtttg cgggcagcaa cccggcagcg ttccatgccg aagatggcag
    12181 cggttaccag ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtagc
    12241 ttcacgtctg attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa
    12301 aatgcgcgcg gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctttacga
    12361 gaagataact aaagcactgg cttgatactt ttgagccaaa atgcctgatg cgacgcttga
    12421 cgcgtcttat caggcctaca actccgttga aattgctggt tttgtaggct ggataaggcg
    12481 ttcacgccgc atccggcagc cgtgcccaac tgcctgatgc gatgcttgac gtgtcttatc
    12541 aggcctacaa gtccgttgaa aatgctggtt ttgtgcgcca catccggcat attaaccatg
    12601 acgccgaatc agttgttcag aatgcccacg ccgcattacc cgttgcaata cttccgcttc
    12661 cagctccgct aatcgcaccg accctaatcg acgcggacgg gggatatcca ctgtcaaatc
    12721 cagactaatt tttccctctt caattaacaa cacccggtca gccattgcta ccgcttcgct
    12781 cacatcatgc gtcaccaaca gtacggtaaa gccgtgctgc tgccaaagtg acacaatcaa
    12841 atcctgcatc tcgagtcgcg ttaaggcgtc cagtgccccg agcggttcat caaacaacaa
    12901 taaacccggt cgatgaatca acgctcttgc cagcgccaca cgctgtttct gcccgccaga
    12961 aagtgcggca ggccattccc cggcgcgatt ctccagtcct accgcagcca acgcccgacg
    13021 cgcggcatcg cgccactgac ctttaaggcc taacccaacg ttatcaatca ccgatttcta
    13081 cggcagcaga cgcgcatcct gaaacatcat tcgcgtatca tcctgaattt cagccagcgg
    13141 tgtggtgccc gctaacacat cgcctacggt tggcgtttcc agaccggcca gcaggcgcag
    13201 cagggtactt ttaccaccac cgctgcggcc caccaccgcc acaaactgac ctgccggaat
    13261 atgtaaatcc agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag
    13321 caacaatggt gtgccctggt tcagacgagc agtattcata tcgtggcctc cttcaaatga
    13381 taagccgggt tccagctcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt
    13441 ttgccgagca gggcgtaaag aataatagcg actaccacca cgtctgtttg caaaaactct
    13501 cgcgcattca tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc
    13561 agcgtcagcc acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca
    13621 tcgggcagga tcacatggat aaacagcggt atgccggata atccatagct acgcgccatc
    13681 tccaccagcc cgcgatcgat attacggatg ccatgccagg tgttgatata aatggggaac
    13741 agcgtaccga gcgccaccag aaagattttc gcggattcat caatgccaaa ccacaaaatc
    13801 accagcggga tcagcgccag atgctgccac agttcgccgc tggcggagag cgtccagaac
    13861 gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg acagccagcc aaccgaggag
    13921 gccagttgcc acaccgccac gatgcccacc ggtaaaaacc agggggcaac gcgcaataac
    13981 tacttcttca ctggcgttgc catcatcgtg ttccttagct ttgcgcgact ttacggggga
    14041 taaaatcatt cgccaccgct tcgccttgcg gattcagcgg ctgcggctgg ggaatttccg
    14101 gaatggcgac atccagatgt gggaacagca actcgccaac ccgatacgct tcttccagat
    14161 gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc ggcgtattcg ttgattcgcg
    14221 cggcgaccgt aggaccatcg cccaccagcg ccgttccggc accgccgcgc actaagccaa
    14281 cgcccgccca taaattgggg ctgatctcca gattgtcgcg cttgccgtta tgtagcgccg
    14341 ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc ggcttgtgct ttggcgatag
    14401 tctcatcatc aagatgcgag attaaccgct cggcggcctg ccacgcttcg tcgttagttt
    14461 cacgaacaat cacatgcaga cgaataccga aacgaatttt gcgcccatac gcggcagctt
    14521 tcgcccgcac ttgttcgatt ttctctttaa caagttccgg cggttcgccc caggtgaggt
    14581 agagatcaac ttgttctgcc gccagctcct gggcgacatc tgacgatccg ccaaagtaaa
    14641 gcggaggata aggctgttga atcggcggga agagcagttt tgctccgcgc acatgaatat
    14701 gtttaccgtt gaaattgacg gtttccccca gcaataagcg ccgccagacc tgggtaaatt
    14761 ccgccgaggc ttcgtagtgc tcgctatgat caaggaacac gccgtcgcct gccagctctt
    14821 gtggatcgct gcctgtgacc agattaaaca acgcacgacc atttgagaga cggtcaagcg
    14881 tggcggcctg gcgggcggca acagtaggtg aggttacgct gggacgcagg gcgacaagaa
    14941 acttcagccg ctgcgtcacc gggatcatcg acgctgcaac cagccacgca tcttcacacg
    15001 atcgccccgt tgggatcagc acaccggtat agcccagacg atccgccgct tgcgcaattt
    15061 gttgcagata accgtgatca accgggcgtg aaccttcttc cgttcccaga tagtgcccgt
    15121 caccgtgggt cggtaaaaac cagaacatat tcagactcat aattgttttc cttccagttg
    15181 agtgggctgc cagatgcgct ggcgaatatc gacttttttc ggcaccagac ggttttcata
    15241 aaacagatct gccgtttgct gctgtaaggc ggcaacctcg gcgttaaccg gtttgatggt
    15301 ggtaggaggg cgatgatcta agtaagaggc aatcaccggt gccggtaagc ccatcgtttt
    15361 tgccagtaaa gcgatgcttt gttcgcgctg gctgcgggtt aacgcatcgg cctcactaaa
    15421 ggttgccagt acgccctgaa taaaagcgcc gtttttttct gcatagggac gagctgccag
    15481 ataaaacgat ccagtttgat tgagatcggt gccgtctttc agcacccgca cgccgccctg
    15541 taataacgca gcggagtagt agggatccca gatagcccag gcgtcaacgt taccttgctg
    15601 gaacgcggcg cgggcaccgg caggcgtcag gtaagtgggc tggatatcgg tgaatttaag
    15661 tccggcctga cgcagtgcac gcagtaaaag gttgtgtgaa ctggaacctt tctgaaaggc
    15721 aactttgtga cctttaagat cggctacggt tttgatcggg ctgttttctg ccaccagaat
    15781 cacttcggct ttgggcttcg gtggctcgac gcccacatat accaaatcag ccccggcagc
    15841 ctgggcaaag attggcggaa tatccccggt actgccgaga tcaatactgc caacgtttaa
    15901 cgcttccaac atttgcggac ccgcggggaa ctccacccag gagatttttg attgcggata
    15961 gcgtttttcc agtaactggt ggctttttgc cagcaccata ccaatactgc ctttctgata
    16021 gcctatacgt aacgcttcag gcgaggattc tgcagcaacc gcaaaagtag agacgctaag
    16081 caatcccgcc agcgccagtt taatgatgtt acgcatgggc attacctcgc agagacagaa
    16141 ggtcaggaac ctgaacatcg cggcggtgca atgcctgcca gaaagtttct agcgcggtat
    16201 caagatgggg ttgcagattt ggcgtgaact ggggtctgtg atggtaatca attaattgtg
    16261 agtcatcggc aaacacgccg tgcaggatct cctgagcttt cagtgcgctt aaaactggtt
    16321 taagggcata atcgaccgcc agcagatggg ccacggtacc gcccgtcgcc agcggtagca
    16381 ccactttgcc ttgcaaagcg cgctctggca gcaggtcgag cagggttttc agcgcgccgg
    16441 aataggcggc tttatacaca ggcgtggcga caatcagccc atcagcctgt tgcagctgtt
    16501 cggtgaaggt cttgagtgcc ggactatcga aacgagcata aagcagatct tccggggcga
    16561 agttttgcag attccagtga taaacctcta catctaggcc atttagtttt tcccgcgcat
    16621 attccagcaa ggagctggag cgagaaggaa agcgaggact acccgccagg gtgatgacac
    16681 gcatactctc tccttataac caattgttct tttttttgtt aacattgata acaattcggt
    16741 cagtctgtcg gagagaca
//
LOCUS       DADLZM010000090        16507 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLZM010000090 DADLZM010000000
VERSION     DADLZM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.091"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(283..987)
                     /locus_tag="JNY73_002714"
     CDS             complement(283..987)
                     /locus_tag="JNY73_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY8048674.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNY73_002715"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNY73_002715"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY8048675.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNY73_002716"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNY73_002716"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY8048676.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNY73_002717"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNY73_002717"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY8048677.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNY73_002718"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNY73_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY8048678.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNY73_002719"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNY73_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY8048679.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="JNY73_002720"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="JNY73_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY8048680.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNY73_002721"
     CDS             complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNY73_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY8048681.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNY73_002722"
     CDS             complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNY73_002722"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY8048682.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPSSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNY73_002723"
     CDS             complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNY73_002723"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY8048683.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNY73_002724"
     CDS             complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNY73_002724"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY8048684.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIACFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNY73_002725"
     CDS             complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNY73_002725"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY8048685.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11614..12903
                     /gene="bioA"
                     /locus_tag="JNY73_002726"
     CDS             11614..12903
                     /gene="bioA"
                     /locus_tag="JNY73_002726"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY8048686.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12881..13351
                     /locus_tag="JNY73_002727"
     CDS             12881..13351
                     /locus_tag="JNY73_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY8048687.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13666..13749
                     /locus_tag="JNY73_002728"
                     /pseudo
     CDS             13666..13749
                     /locus_tag="JNY73_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            13905..14906
                     /locus_tag="JNY73_002729"
     CDS             13905..14906
                     /locus_tag="JNY73_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8048688.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14940..16496)
                     /locus_tag="JNY73_002730"
     CDS             complement(14940..16496)
                     /locus_tag="JNY73_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HAY8048689.1"
                     /translation="MLKYDPDQFGPDLIEQLAQSGKYSQDNTKGDAMIGVKQPLPKAV
                     LRTQHDKNKEAISILDFGVIDDGVTDNYQAIQNAIDAVASLPSGGELFIPASNQAVGY
                     IVGSTLLIPGGVNIRGVGKASQLRAKSGLTGSVLRLSYDSDTIGRYLRNIRVTGNNTC
                     NGIDTNITAEDSVIRQVYGWVFDNVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHF
                     LGQCVSVSVSSCHFSRGNYSADESFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFK
                     NAVYVHDCLDLHMEQPDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFR
                     TPTSTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDI
                     NECVSFDKCIFNKPLCYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGI
                     INVPVAVGATSGSAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVA
                     LPSMVEYLAI"
BASE COUNT         3938 a         4341 c         4083 g         4145 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttccgtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atctaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
     6241 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
     6301 cgccaatttg tggaatggtg acgtgagatt catcgacgac cagcagccca tcggcaggca
     6361 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
     6421 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
     6481 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg
     6541 cggccagttc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
     6601 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
     6661 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
     6721 catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct
     6781 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
     6841 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
     6901 ccagaccata aatcgcggaa acagacgcca ctacaatcac atcacgccgc tccagcatcg
     6961 ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
     7021 tgaaggtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga
     7081 aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
     7141 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
     7201 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
     7261 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
     7321 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
     7381 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
     7441 atcaccataa tatttctgat acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
     7501 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
     7561 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgtacag tgactgccat
     7621 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
     7681 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
     7741 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
     7801 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
     7861 ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aaatacagaa
     7921 tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
     7981 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     8041 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
     8101 tccggcgtgt tgtattacct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     8161 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
     8221 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     8281 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgggc
     8341 gctgatgatg tgcggcgagg agggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8401 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
     8461 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
     8521 cgcggcttgt aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt
     8581 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8641 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8701 ttcgcgggtc gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg
     8761 cactgagcgc atcatcaaac catagtgtga tgagctgaat atgatgttga taacgcacgc
     8821 cgttcagcga ctcttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
     8881 ccaccgcctg ccgcgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8941 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
     9001 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     9061 gggattcgat atctcccgcc agataatgat ctgcggcatc tttatggcgg gcttgcgcaa
     9121 gcattggcgg cgagagatct aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc
     9181 tcatccagcc aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtggaagca
     9241 ttgccagtaa ggcgtcagca ctctggcgct gtagatctgc atgttgctca tagtgtgcgg
     9301 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9361 agcagacgat cgatatcctg catttcatgc gcagcggtta gcgttaagcg cagtcgcgca
     9421 gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt
     9481 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
     9541 catgaatcag ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaaca cgcaatgagt
     9601 gacaccagtt tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagcgacgca
     9661 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
     9721 tgcagcagat aatccgccac cgtacttgag caaagcaccg cagccccact gacgccaaat
     9781 cctttgccaa aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg
     9841 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
     9901 tgctgttgcg ttacctgctg gatttccgcc agtggcgcgc tatcgccgtc catgctgaac
     9961 acgccttctg tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
    10021 tgagcgacat cgttatgagt aaaacggcga agctgcgacg ggcttaaact ggcagcctcc
    10081 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
    10141 gcaatgactg cctgattcgc ggcaaaacca gagataaaca gcagcgcccg cgaatagcca
    10201 agccactcgg ccagttcttc ttccagcgcc tgatgcacca cgctataacc gctgacgtga
    10261 ccggagccgc cgctaccgat gccaaattgc tccgccccct gcttccaggc acggataatt
    10321 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    10381 tccgccacca gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg
    10441 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    10501 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg tccaagacgt tgctgttgtt
    10561 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10621 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    10681 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10741 cagaaaggcg cacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtgcgaataa
    10801 aatcaaaggc atcaacgtca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca
    10861 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    10921 cgcgatcttt taccgtttcg cctaagccca caatgccacc tgagcagact ttgatcccgg
    10981 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgtgtg gtgatgatat
    11041 tgccgtaaaa ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg
    11101 cgaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    11161 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    11221 tccatgccgc gcccatacag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact
    11281 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11341 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaacgtgc
    11401 tgacctgcac ctgacgagga tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca
    11461 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11521 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag gcttgtaaac
    11581 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    11641 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg
    11701 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11761 tcgtcctggt gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag
    11821 acgcaaattg atgccatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc
    11881 gagctatgcc gcaaactggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg
    11941 gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc
    12001 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    12061 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    12121 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgc
    12181 gatatggttg gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc
    12241 attgagccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12301 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12361 ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt
    12421 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac acttaccacg
    12481 cgcgaggttg cagaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12541 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc
    12601 ggcgaatggc agcagcaggt ggcggctatt gaagtgcagc tgcgcgagca actggcacca
    12661 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa
    12721 accactcgtc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggggtatgg
    12781 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12841 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    12901 taatggcgat aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa
    12961 tatttcaccg catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac
    13021 ctgctacgac ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt
    13081 acccgctgat acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga
    13141 cggcgttttg cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc
    13201 gaaaggcgaa acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga
    13261 tgtcgatgaa ggaggcagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc
    13321 aagcgcatcg attaccgcga tgtttagtta atcactctgc cagatggcgc aatgccatct
    13381 ggtatcactt aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa
    13441 gttagtataa aaaagctgaa cgagaaacat aaaacgctgt aaatatcaat atgttatgaa
    13501 agatttagtc taaataatag actgcataat actacaaaac acaacatatc cagtcactat
    13561 gaatcaacta cttagatagt attagtgacc tgaggcagag cattagcgca aggtgatttt
    13621 ttgtcctctt gcgctaattt tttgtcaacg gactgggtta gttgaattta caaccatgct
    13681 aagtatctcg ataagcgcag agaaatgatg cagtggtggg cggattggct tgatgagaag
    13741 gttaggtagg caaatccatg cctgagcaga gccttgccct ctacctgagc ccatgctcat
    13801 tctttggcct caacgcgagc atgacctcac tggctggacc caaaatggac tatgacagaa
    13861 agatctgata tttttcctcg caaaaactta gaggctttat tttaatgcat aagagcaact
    13921 gctttgacac ggcaaggctt gtggcagcta tgatggttct ggtcagtcac cactacgcgc
    13981 tctctgggca gccggagccg tacctgttcg gctttgaaag tgctggcggc attgcagtta
    14041 tcatattctt ttcaatatca ggatatctga tttcaaaatc agctatcagg agtgattcct
    14101 ttattgattt catggctaaa agagcgagaa gaatattccc ggcgctggtc ccttgctcta
    14161 tactgacata ttttttattt ggatggatac ttaacgactt cagcgcagaa tattttagcc
    14221 atgacattgt caggaagacc ataagctcta tttttatgag tcaggctcca gatgctgaca
    14281 ttacatcaca tttaattcat gccggaatta acggcagttt gtggacccta ccgcttgagt
    14341 tcctgtgtta tataataacg ggagtagcag ttgccctact aaagaacgga aaagcattca
    14401 tagttatact gttggttttt gtttctcttt cattaattgg aagtgtgtca gaaaacaggg
    14461 atgtaatgtt ctctattccg ttatggctgt atcctttgcg tggactggcg ttcttttttg
    14521 gtgccactat ggcaatgtat gagaagtcat ggaatgtaag taatgttaaa attactgtag
    14581 tttctttgct tgctatgtac gcctacgcta gttatggcaa aggcattgat tatactatga
    14641 cttgctatat cctggtttca ttctcgacaa tagccatatg cacatccgtt ggcgacccac
    14701 tggttaaggg caggtttgat tactcgtatg gtgtgtacat ttatgcattc ccggttcagc
    14761 aggttgttat aaacacattg cacatgggtt tttacccgtc aatgttgtta agtgccgtga
    14821 ctgttctttt cttgtcccat ctttcatgga atctggttga aaaaagattc ctgacacgaa
    14881 gttcgcctaa attatccctg gattgaacaa taaaaggggc catatggccc ctaaaataat
    14941 cagatggcca gatactcaac cattgaaggc agcgcgactc ctactgggct tgggcgagta
    15001 acattaattg tcaccacgga aacactaacc ttgtcaccac ttgatgcagg gtcaccaaaa
    15061 aggcttctta ctctgtatgt caggttcccc gggttcggta tagctgcaga tccgcttgtg
    15121 gcacctaccg caactgggac attaatgatc ccatttacag acggtacgcc tgaacagcca
    15181 cgatacgtat tgtatctgcc ttccggtttc tgcacctcgg taatgccagc caaataacag
    15241 tcagtaactg acacgccacc gctacgaaga tagcatagag gcttattgaa tatgcactta
    15301 tcgaaagaaa cacattcatt gatgtcgaca atatttacag cccattcatc accagttaac
    15361 gtacaaccac ttacaaagac gaagatcgcc gactgttcta tcgctatact ttgattgttt
    15421 gcagccgtgt ttttgtttgc agtgttgata tgaacaccac tgacaatttt atgtgactgc
    15481 gttgaggtcg gcgttctaaa atatatcccc gtaaattgtt cagtgccatc ggcatctgct
    15541 gctatccatg agtttgagaa agaaaatcct ccgtttacat tctctataac cacgcctgtt
    15601 gaaccgcaat aatctaaatc cggttgttcc atatgcaaat caaggcaatc atgaacatag
    15661 acggcatttt taaaaccaat gcacatggtc tcactgtcta aaattattgc ttctgaccta
    15721 actgcctctg acgaccacgc atacgtttga ggctgaatcc tgatgccaaa gctttcatca
    15781 gcagaataat ttcctctgct gaaatggcag gagctgacac taacacttac gcactggcct
    15841 aaaaagtgaa gaccgactct acaggttcca gcctgacatg ctataaattt tgagtgccag
    15901 agcccttgca ttaaataagc ggtttcaact tcattcacca ttacattatc aaatacccag
    15961 ccataaacct gtctgatgac agagtcttct gctgtaatgt ttgtgtcaat accattgcag
    16021 gtgttattac cagttactcg tatatttctc agataacggc cgatagtgtc tgaatcataa
    16081 gacagcctta acacagatcc tgtaagtccg ctttttgctc ggagttgcga tgccttacca
    16141 acccctctga tgttaacacc gccaggaata agcaaagtgg atccaacaat ataccccacc
    16201 gcttggttgc tcgcagggat aaacagctcc ccgccggagg gtagtgaagc aacggcatct
    16261 attgcatttt gtattgcctg gtaattatct gtcacaccat catcaataac accaaaatcc
    16321 aggatactta ttgcttcttt atttttgtca tgctgagttc ttaaaactgc ttttggtaaa
    16381 ggctgcttga cgccaatcat ggcatcgcct ttggtgttat cctgcgaata cttaccggat
    16441 tgagctagtt gctcaattaa atccggaccg aactgatcag ggtcatactt cagcaaattc
    16501 ggaaaat
//
LOCUS       DADLZM010000091        16396 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLZM010000091 DADLZM010000000
VERSION     DADLZM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.092"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(71..418)
                     /gene="grxD"
                     /locus_tag="JNY73_002731"
     CDS             complement(71..418)
                     /gene="grxD"
                     /locus_tag="JNY73_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY8048690.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            753..1580
                     /locus_tag="JNY73_002732"
     CDS             753..1580
                     /locus_tag="JNY73_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY8048691.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1708..2289
                     /gene="sodB"
                     /locus_tag="JNY73_002733"
     CDS             1708..2289
                     /gene="sodB"
                     /locus_tag="JNY73_002733"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY8048692.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2435..3604)
                     /locus_tag="JNY73_002734"
                     /pseudo
     CDS             complement(2435..3604)
                     /locus_tag="JNY73_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3770..3859)
                     /gene="ynhF"
                     /locus_tag="JNY73_002735"
     CDS             complement(3770..3859)
                     /gene="ynhF"
                     /locus_tag="JNY73_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY8048693.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4158..5183
                     /gene="purR"
                     /locus_tag="JNY73_002736"
     CDS             4158..5183
                     /gene="purR"
                     /locus_tag="JNY73_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY8048694.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5180..6112)
                     /locus_tag="JNY73_002737"
     CDS             complement(5180..6112)
                     /locus_tag="JNY73_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8048695.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6225..7436
                     /locus_tag="JNY73_002738"
     CDS             6225..7436
                     /locus_tag="JNY73_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY8048696.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7727..8875
                     /gene="cfa"
                     /locus_tag="JNY73_002739"
     CDS             7727..8875
                     /gene="cfa"
                     /locus_tag="JNY73_002739"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY8048697.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8915..9556)
                     /gene="ribE"
                     /locus_tag="JNY73_002740"
     CDS             complement(8915..9556)
                     /gene="ribE"
                     /locus_tag="JNY73_002740"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY8048698.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9771..11144
                     /gene="mdtK"
                     /locus_tag="JNY73_002741"
     CDS             9771..11144
                     /gene="mdtK"
                     /locus_tag="JNY73_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY8048699.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11185..12441)
                     /locus_tag="JNY73_002742"
     CDS             complement(11185..12441)
                     /locus_tag="JNY73_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048700.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYCLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12749..12825
                     /locus_tag="JNY73_002743"
     tRNA            12749..12825
                     /locus_tag="JNY73_002743"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12783..12785,aa:Val,seq:gac)
     gene            12830..12906
                     /locus_tag="JNY73_002744"
     tRNA            12830..12906
                     /locus_tag="JNY73_002744"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12864..12866,aa:Val,seq:gac)
     gene            13014..13319
                     /locus_tag="JNY73_002745"
     CDS             13014..13319
                     /locus_tag="JNY73_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY8048701.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13445..15049
                     /locus_tag="JNY73_002746"
     CDS             13445..15049
                     /locus_tag="JNY73_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY8048702.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(15061..15873)
                     /locus_tag="JNY73_002747"
     CDS             complement(15061..15873)
                     /locus_tag="JNY73_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048703.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15877..16396)
                     /locus_tag="JNY73_002748"
                     /pseudo
     CDS             complement(15877..16396)
                     /locus_tag="JNY73_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
BASE COUNT         4054 a         4240 c         3897 g         4205 t
ORIGIN      
        1 gaggttttac ggcgcaccgg ataaaggact gacggatgcc cgttcgcatc cgtcagtatt
       61 gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca gtttctttga
      121 tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa ccgccgacca
      181 gctcgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc ggcagttccg
      241 cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca catgcggcaa
      301 gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa cctttcatgt
      361 acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg gtgctcattg
      421 tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt cagtgcggca
      481 tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct atttgttgca
      541 tttcaaatat tcgtttcttc tctgatgcgc ggcaaactgg cgtatttata accattttta
      601 gctttcactg ctgcattttt ttgcgctcgc caacgaaacg tattttttaa caataaaagc
      661 tattaacttt ctcttcttct atgcattaga atcatcaagt tttgtaaatc agacgcaggc
      721 atgatagacc tgccttaaca gagggacgct cagtggcgcg gataaaccgt atttcgatca
      781 cgctctgtgc tttacttttt accaccctgc ctttaacgcc tatggcccat gcttcaaagc
      841 aagccaggga gagttctgct accgctcata tcaccaaaaa agcagataaa aagaaaagca
      901 cggcaaccac caaaaagacc cagaaagcca gccagaaaac agcgaaaaaa gccgccagta
      961 aaagtacgac caaaagcaaa accgcttctt ccgttaaaaa atcttccatt accgcttcta
     1021 aaaacgccaa aactcgcagc aaacacgccg tcaataaaac ggcctcagcc agctttaccg
     1081 aaaagtgtac caaacgtaag ggctataaat cgcattgtgt gaaagtcaaa aatgccgcgt
     1141 caggaaccct tgccgacgcg cacaaagcga aggtgcaaaa agccacgaaa gtggcaatga
     1201 ataaactgat gcagcaaatt ggtaagccat atcgttgggg cggcagctca ccgcgtaccg
     1261 gttttgattg cagcggcctg gtttattacg cttataaaga tttggtgaaa attcgtattc
     1321 cgcgtacggc gaatgaaatg tatcacctgc gtgatgcagg acctatcgaa cgtagtgagc
     1381 tgaaaaacgg cgacctggtc tttttccgta ctcagggacg cggtacagcc gatcatgtcg
     1441 gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg cacaggtcag gaaattcaaa
     1501 tcacttctct cagtgaagac tactggcagc gccactatgt tggcgctcgt cgggtaatga
     1561 ccccaaaaac acttcgctaa aactttaccc tgttgttacg gcaacagggt aagttcatct
     1621 tttgtctcac cttttaattt gctaccctat ccatacgcac aataaggcta ttgtacgtat
     1681 gcaaattaat aataaaggag agtagcaatg tcattcgaat tacctgcact accatatgct
     1741 aaagatgctc tggcaccgca catttctgcg gaaactatcg agtatcacta cggcaagcat
     1801 catcagactt atgtcactaa cctgaacaac ctgattaaag gtaccgcgtt tgaaggtaaa
     1861 tcactggaag agattattcg cagctctgaa ggtggcgtat tcaacaacgc agctcaggtc
     1921 tggaaccata ctttctactg gaactgcctg gcaccgaacg ccggtggcga accgactgga
     1981 aaagtcgctg aagctatcgc cgcatctttt ggcagctttg ccgatttcaa agcgcagttt
     2041 actgatgcag cgatcaaaaa ctttggttct ggctggacct ggctggtgaa aaacagcgat
     2101 ggcaaactgg ctatcgtttc aacctctaac gcgggtactc cgctgaccac cgatgcgact
     2161 ccgctgctga ccgttgatgt ctgggaacac gcttattaca tcgactatcg caatgcacgt
     2221 cctggctatc tggagcactt ctgggcgctg gtgaactggg aattcgtagc gaaaaatctc
     2281 gctgcataat aactgatggc aaatgcagca ttgcctgatg cgcttcgctt accatgccaa
     2341 cgcggatgag gcgttaacag cgcattcggc aagataaatc gcacgttgtc agcaactgta
     2401 acgcagaagg ttatccttct gcgtttttgt ttaattagct gttagcaacg caaactgttt
     2461 caggttgttt tctggctgac ataaacacca gcaataatgc cagtcccgcg acaatcgccc
     2521 ccgtcaccgg cacaaagctg tatcccagcc cagcggaaat taccgcacca ccagcagctg
     2581 ctcccagcgc atttccaaga ttaaaggcac caatattgac tgatgaagac agacctggcg
     2641 cttcactggc gacatgcatc acgcgcatct gtaacggagg tacaaccgca aaggttgctg
     2701 cgccccacac caccatgcta atagctgcgc cgaactcatt gcgggccagg aacgggattg
     2761 ccagcataat caccatcagc agcaacaaaa agcctttcaa cgtgccgtta actgaacgat
     2821 ctgccagttt gccgccgaga tagttaccga tagagaatcc gacaccaatc agcaccagca
     2881 ttgccgtgac gaacaccggt gttgcgtggg taatactttg cagtaccgga gagatatagg
     2941 tgtagagggt aaacattgca ccagctccca gtaccgtcgt cagcaatgca gacagcacct
     3001 gcggacgcat taataccgcc agctcttttt tcacttcagg tcgtgcccct gtaccacctt
     3061 taggtaatga gaagaacaga cttaccattg aaatcactcc cagccccgcc gttgccagaa
     3121 atgacatccg ccagccgatg gtttcaccca acctggtcgc cgccggcacg ccaccgatat
     3181 ttgccagggt taaccccata aacatagtgg caactgcgct ggcctgttta tgttttggca
     3241 ccacgcttgc ggccacgact gaacccaaac caaaaaatgc tcagtgattc aggctggtca
     3301 aaatacgtga aagcatcagg gtcatataat ccggcgcgat ggcggaaaga acgttgccta
     3361 gcgtgaaaat tgccatcagg aaaatcaacg cactgcggcg ggcacgatga gaaagtagaa
     3421 gcgtcatcag cggcgcgccc accattacgc caactgcata ggcactgatt aacattccgg
     3481 cagcgggaat cgagacatcc acaccgcgcg caatgacggg caacaagccc attggcgaga
     3541 actccgttgt cccgatacca aacgcgccaa tcgccagcgc cagcaacgga tagttaattt
     3601 tcatgcctta tctccacctc ttcgcgtcat tacgcgatat tcattaaagt ggcgaaagca
     3661 tgacagcaat cacaaaaaaa tgaaaataac aaaaagagaa aacacttttg ccattttgct
     3721 aacaaacagg aaggagatgc gagggagaac gcgctccctc gagaggaaat cagtgcagcg
     3781 cggcagtcaa acccacggct acgatcaaac cgaggacgat aatcgttgtt accagtgaaa
     3841 atttaaggtc ggtgctcatc aagttttctc cttttttatt accacacaaa aaatgatatt
     3901 acgcattttt acacactgtg atgaaaaaat ctcccgtcat ttataatgat aagtgttttt
     3961 accacttccc cttttcgtca agatcggcca aaattccacg cttacactat ttgcgtactg
     4021 gccattgacc ccttcctgac gctccgtgtc gtttttccgg cgtaccgcaa cacttttgtt
     4081 gtgcgtaagg tgtgtaaagg caaacgttta ccttgcgatt ttgcaggagc tgaagttagg
     4141 gtctggagtg aaatggaatg gcaacaataa aagatgtagc gaaacgagca aacgtttcca
     4201 ctacaactgt gtcacacgtg atcaacaaaa cacgtttcgt cgctgaagaa acgcgcaacg
     4261 ccgtgtgggc agcgattaaa gaattacact actcccctag cgcggtggcg cgtagcctga
     4321 aggttaacca caccaagtct atcggtttgc tggcgaccag cagcgaagcg gcctattttg
     4381 ccgagatcat tgaagcagtt gaaaaaaatt gcttccagaa aggttacacc ctgattctgg
     4441 gtaatgcgtg gaacaatctt gagaaacagc gggcttatct gtcgatgatg gcgcaaaaac
     4501 gcgtcgatgg tctgctggtg atgtgttctg agtacccaga gccgttgctg gcgatgctgg
     4561 aagagtatcg ccatatccca atggtggtga tggactgggg tgaagcaaaa gctgacttca
     4621 ccgatgcggt cattgataac gcgttcgaag gcggctacat ggccgggcgt tatctgattg
     4681 aacgcggtca ccgcgaaatc ggcgtcatcc ccggcccgct ggaacgtaac accggcgcag
     4741 gccgccttgc cggttttatg aaggcgatgg aagaagcgat gatcaaggtg ccggaaagct
     4801 ggattgtgca gggtgacttt gaacctgaat ccggttatcg cgccatgcag caaatcctgt
     4861 cgcagccgca tcgccctact gccgtcttct gtggtggcga tatcatggca atgggcgcac
     4921 tttgtgctgc tgatgaaatg ggcctgcgcg tcccgcagga tgtttcgctg atcggttatg
     4981 ataacgtgcg caacgcgcgc tattttacgc cggcgctgac cacgatccat cagccaaaag
     5041 attcgctggg tgaaacagcg ttcaacatgc tgttggatcg tatcgtcaac aaacgtgaag
     5101 aaccgcagtc cattgaagtg catccgcgtt tgattgaacg ccgctccgtg gctgacggcc
     5161 cgttccgcga ctatcgtcgt taatcacccg ttgcgggagt ctcttccggc tcccgcagcc
     5221 actccttatt cagcgtctca ctatcgccga gatactcaag cagccaggtt aacgcaggcg
     5281 acatatcatt ttgctgccat gtcagacaac atgccgaatc cgggaagggg ttttccagtt
     5341 caagtgctac ccacttcccc tcattaagcc acggtttggc gaaatgtgtt ggcaccatcc
     5401 ctatgcataa tcctgccgag atacaggttg ccgatgattc ccagtcaggt acgacaagtc
     5461 ttttttggtt atccagcaac caggtaatac gtttaggtag cgttcgcgag gtgtcttcgc
     5521 gcaccaacga cggccagttg cgcaacgtat catcgctgaa cgggccatcc atcaacgcca
     5581 gcgggtggtg actggcaaca acgcaactcc agcttagcat ccccatatcc cggaaggcat
     5641 aacgaccgcc taccggaatc gcgcgtgttg cgccaattgc cagttccacg cgcccgtcgg
     5701 aaagcgcatc ccagacaccg ttgaacactt cctgaaagac aagaagttca acatcatcaa
     5761 aatggcgata aaaatcaacg atcatctgcc gtgtacgttc tggcctgaca atattatcca
     5821 ctgcgatagc taactgaccg cgccagccgt tcgctatctg ctgacattgc tggcgggtga
     5881 tctgcatttt tttgacaaca gagcgccctt ctttgagaaa ccacgctcca gcagcggtca
     5941 gctccacatc acggtgccgt cgttcaaaga gcggtaccgc cagccactct tccagctgac
     6001 gcacggtata gctgaccgca gaaggaacgc gatgcagctc ctgtgccgca gcgctaaaac
     6061 taccattacg cgctaccgca tcaacaactt cgagtgaata ttctgaccac atagtcagcc
     6121 tgcaaaattt ttgaaaccag tcatcaaata ttaccgtttc acaacactaa tttcactccc
     6181 tacactttgc ggcggtgttt aattgagaga tttagagaat atacatgcaa cctgggaaaa
     6241 gatttttagt ctggctggcg ggtttgagcg tactcggttt tctggcaacc gatatgtatc
     6301 tgcctgcttt cgccgccata caggccgacc tgcaaacgcc tgcgtctgct gtcagtgcca
     6361 gccttagtct gttccttgcc ggttttgccg cagcccagct tctgtggggg ccactctccg
     6421 accgttatgg tcgtaaaccg gtgttattaa tcggcctgac aatttttgcg ttaggtagtc
     6481 tggggatgct gtgggtagaa aacgccgcca cgctgctggt attgcgtttt gtacaggctg
     6541 tgggggtctg cgccgcggcg gttatctggc aagcattagt gacagattat tatccttcac
     6601 agaaagtcaa ccgtattttt gcgaccatca tgccgctggt gggtctgtct ccggcactgg
     6661 ctcccctgtt aggaagctgg ctgctggtcc atttttcctg gcaggcgatt ttcgccaccc
     6721 tgtttgccat taccgtggtg ctgattctgc ctattttctg gctcaaaccc acgacgaagg
     6781 cccgtaacaa tagtcaggat ggtctgacct ttaccgacct gctacgttct aaaacctatc
     6841 gcggcaacgt gctgatatac gcagcctgtt cagccagttt ttttgcatgg ctgaccggtt
     6901 caccgttcat ccttagtgaa atgggctaca gcccggcagt tattggttta agttatgtcc
     6961 cgcaaactat cgcgtttctg attggtggtt atggctgtcg caccgcactg cagaaatggc
     7021 aaggcaagca gttattaccg tggttgctgg tgctgtttgc tgtcagcgtc attgcgacct
     7081 gggctgcggg cttcattagc catgtgtcgc tggtcgaaat cctgatccca ttctgtgtga
     7141 tggcgattgc caatggcgcg atctacccta ttgttgtcgc ccaggcgctg cgtcccttcc
     7201 cacatgcaac cggtcgcgcc gcagcgttgc agaacactct gcaactgggt ctgtgcttcc
     7261 tcgcaagtct ggtagtttcc tggcttatta gtatcagcac gccattgctc accaccacca
     7321 gcgtgatgtt atcaacagta gtgctggtcg cgctgggtta catgatgcaa cgttgtgaag
     7381 aagttgactg cccgaatcat ggcaatgccg aagtcgctca tagcgaatca cactgatcta
     7441 tatcgatata cttatactta ggctgctaac aaaattttgt tgtatgattg aaattagcgg
     7501 cctatactaa tttcgagttg ttaaagctac gataaatatt atgtttttac ggggacagga
     7561 tcgttcccga ctcactatgg atggtcattt cggcaagggt tcctcctttc cctctgttct
     7621 acgtcggatt atagactcgc ggttttttct gcgagatttc tcacaaagcc caaaaagcgt
     7681 ctacgctgtt ttaaggttct gatcaccgac cagtgatgga gaaactatga gttcatcgtg
     7741 tatagaagaa gtcagtgtac cggatgacaa ctggtaccgt atcgccaacg aattacttag
     7801 ccgtgccggt atagccatta acggttctgc cccggcggat attcgtgtga aaaaccccga
     7861 tttttttaaa cgcgttctgc aggaaggctc tttggggtta ggcgaaagtt atatggatgg
     7921 ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa gtcttacgcg caggtctcga
     7981 aaaccaactc ccccatcatt tcaaagacac gctgcgtatt gccggcgctc gtctcttcaa
     8041 tctgcagagt aaaaaacgtg cctggatagt cggcaaggag cattacgatt tgggtaatga
     8101 cttgttcagc cgcatgcttg atcccttcat gcaatattcc tgcgcttact ggaaagatgc
     8161 cgataatctg gaatctgccc agcaggcgaa gctcaaaatg atttgtgaaa aattgcagtt
     8221 aaaaccaggg atgcgcgtac tggatattgg ctgcggctgg ggcggtctgg cgcactacat
     8281 ggcatctaac tatgacgtaa gcgtggtcgg cgtcaccatt tctgccgaac agcaaaaaat
     8341 ggctcaggaa cgctgtaaag gcctggatgt caccattttg ctgcaagatt atcgtgacct
     8401 gaacgaccag tttgatcgta ttgtttctgt ggggatgttc gagcacgtcg gaccgaaaaa
     8461 ttacgatacc tattttgcgg tggtggatcg taatttgaaa ccggaaggta tattcctgct
     8521 ccatactatc ggttcgaaaa aaaccgatct gaatgttgat ccctggatta ataaatatat
     8581 ttttccgaac ggttgcctgc cctctgtacg ccagattgct caatccagcg aaccccactt
     8641 tgtgatggaa gactggcata acttcggtgc tgattacgat actacgttga tggcgtggta
     8701 tgaacgattc ctcgccgcat ggccagaaat tgcggataac tacagtgaac gctttaaacg
     8761 gatgtttacc tattatctga atgcctgcgc aggtgctttc cgcgcccgtg atattcagct
     8821 ctggcaggtg gtgttctcac gcggtgttga aaacggcctt cgagtcgctc gctaaaggct
     8881 attctatcgc cccctcttcg ggggcgagtt cagatcaggc ttctgtacct ggttgattca
     8941 tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc taccactgcc tgagtttgtg
     9001 gatcgatttc aatgttgacg cgtgcgccaa gttttttctt cccaagagtc gtgcgttcca
     9061 gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt gacttcgccg acggtcaggc
     9121 taataccgtc gatgccaata aatcctttgt acaggatata tttcatcaac tgactatcct
     9181 ggactttaaa ccagatctgg cgattatttt ccgaggttaa tattttcgcc acttcagcgg
     9241 tggtcataat atgacctgac attaagtgtc cgccaatttc atcactgaat ttcgccgcac
     9301 gctcaacgtt tacccaatcc cccactttta aatcgccaag attggtaatg cgtaacgttt
     9361 ctttcatcag gtcaaaactg acatggttgc cgttaatttc cgtcacggtc aggcagcaac
     9421 cgttatgcgc cacggaagca ccggtttcca ggccgtccag catgtggtcg ggtaactcca
     9481 ccacatgcgt acgaaaattt ggtttctcgt caatcgacac cagttttgcg gtgccctgta
     9541 caatccccgt aaacatactt acaactcctg aaatcagtta agacattctg ttcagcacaa
     9601 tagcaggtgg aaaacgccct taccagtgaa ggggtaagaa tggctatttt ttcactggag
     9661 aattaataaa tcctcgctac aatagactga atttcccctg cttcttcttt ttgctgccca
     9721 ttcaggcggc tttttagtct ctcatataac tacaaataaa aggtgttcac gtgcagaagt
     9781 atatcagtga agcgcgtctg ttattagcat tagcaatccc ggtgattctc gcgcaaatcg
     9841 cccaaactgc gatgggtttt gtcgataccg tgatggcggg cggctatagt gccaccgaca
     9901 tggcggcggt cgctatcggt acttctatct ggcttccggc gatcctcttt ggtcacggac
     9961 tgctgctggc attaacgccg gttatcgcgc aattaaatgg ttccggtcga cgtgagcgca
    10021 ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt tgtttccgtt ctcattatgc
    10081 tggtgctgtg gaatgcaggt tacattatcc gctccatgga aaacatcgat ccggctctgg
    10141 cggacaaagc cgtgggttat ctgcgtgcgt tgttgtgggg cgcgccggga tatctgttct
    10201 tccaggttgc ccgtaaccaa tgtgaaggtc tggcaaaaac caagccgggt atggtaatgg
    10261 gctttatcgg cctgctggtg aacatcccgg tgaactatat ctttatttat ggtcatttcg
    10321 gtatgcctga gctcggtggc gttggttgtg gcgtggctac tgcggcggtg tattgggtca
    10381 tgttccttgc catggtttct tacattaaac gcgcccgctc catgcgcgat attcgtaacg
    10441 aaaaaggcac cgctaagccc gatcctgcgg ttatgaaacg actgattcaa ctcggtttgc
    10501 cgattgcgct ggcactgttc tttgaagtga cactgtttgc cgtcgtggct ctgttagtgt
    10561 ctccgctcgg tattgttgat gtcgcaggac accagattgc cctgaacttt agttcactga
    10621 tgtttgtgct tccaatgtcg ctggcggcag cggtaactat ccgcgtaggt tatcgtctgg
    10681 gtcagggctc aacgctggat gcgcaaaccg ctgcgcggac cgggcttatg gtgggtgtct
    10741 gtatggcaac cctgacggcc attttcacgg tttcactgcg ggagcaaatc gccctgttgt
    10801 acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt gatgttgctg gcggcggtat
    10861 atcagatttc tgactcaatc caggtgattg gcagtgggat tttgcgtggt tataaagata
    10921 cgcgttccat tttctatatc acctttacag cttactgggt gctgggcttg ccaagcggct
    10981 atattctggc actgaccgat ctggtcgttg aacctatggg gccagcaggc ttctggatag
    11041 gctttattat cggcctgaca tcggcagcca ttatgatgat gctgcgtatg cggttcctgc
    11101 aacgtatgcc gtcagccatc attctgcaac gagcgtcccg ctaataaaga caaggcgcaa
    11161 ccttcacggg ttgcgcctgt gtttttacgc aggctggagc gttgcgccaa tcccgtcttc
    11221 gtctggctgt aatttcagag cgttacacag agtttgccga ctggcatctg ttatctcaac
    11281 gcgtatatcc ccgttaactt cccctttcac attgatgaag tcaggtgaaa tttctttcgc
    11341 actggtttgc aggcaaatcg ctccctctcc gcgcaggcgt ttcactttca gttgcacatg
    11401 cgttgccgca ccagtgatca gccacagttt tccgccattc agattgatgt taccaacaat
    11461 gaaagtgacg ctgtcacctt ttaccgtact gccgtcgaga atcaacacac cgccctgctg
    11521 tacagtaatc gttgattgtt gcagctctgc gccataacca ataatcattg aagcgccgtc
    11581 cagtaatgca acactcccgg tcagttcaac cggtgcgcga tcttcctcta cgacaaaatt
    11641 ttcgtccgcg ctggtgggtg acaacaggat cactgttcca ccttgtatta ccgcattttc
    11701 cagcgtaccg gcatgcactt cagcccgatc ccccacattc accgacaggt tttgcgtctt
    11761 accggaactg tagagctgaa ggccatccgt ccccagacaa taaatggatc cattctcaac
    11821 aatagtgtca gagtcttcac caccgagatt attcatggtg ccaccagcaa gcaacgtatc
    11881 actggctttc ccggccagca taaaaacgcc gccctgatta atctcacaat tgctggcttc
    11941 tccacttgga tacaccaaca attcaccacc ttcatctacc acgaccgctg aggttgtccc
    12001 attaaccaac aggccgccct cttgatcgag aatgattttt tccgcgcgat gtccttccag
    12061 tacacgcagg ttaccgccat tttccagcaa caaaccgtag gcataaccct gatcaacgct
    12121 gaattcacct tcgggatggc ggccattaac cgtagcgagc gtagagagac taattgcgcc
    12181 tccctcggac tgaacaatat ctgtggcaat ggcaccgtcg ctgaccgata gcgtgccatt
    12241 gttaccgtac tgtgtgcatt tggcgatccc accggttgcc actttcaacc agccagcccc
    12301 cgtgaccacg gtattttcgg ctcttccgcc agcttcaaca atcaactcgc cttcagtaag
    12361 ttgcgtcgcg ccaatgacct cgccggtctt aacaatttgc acattcccgt cgctcatcaa
    12421 gtttttcgca tcagatccca tgatttattc ctttgctgca tctgtgtgcc tttattgcta
    12481 cctaagtgta aaggctacgg aggatttatc cacgacagat ttgagatggt ggcaaacaac
    12541 tctgtttaaa ctctgataca cgaattattg ggttgtatca gatgtaaatg cgatcctgaa
    12601 taaaaatcgc ccttgcaaat caacaaaata tagccaattt gcttaaaagc tctgcaaacg
    12661 gtcgaaatag cgcagaaaat tacgttttgc ctcttgccac ctttccactc tgccgctaat
    12721 attcgtcccc gttgtcacct acaacgttgc gttcatagct cagttggtta gagcaccacc
    12781 ttgacatggt gggggtcgtt ggttcgagtc caattgaacg caccatcctg cgtccgtagc
    12841 tcagttggtt agagcaccac cttgacatgg tgggggtcgg tggttcgagt ccactcggac
    12901 gcaccagatt ttcttaatct ggtcttttcc tttttccctc tgtttcttct ctgtatccaa
    12961 tacgttaaaa gatttacact gtcttcgtct gcgttatcag aaggagaatc gctatggcaa
    13021 ctttgttaca atttcatttt gcttttaatg gcccatttgg cgacgcaatg gctgagcagc
    13081 ttaaaccact tgctgagtcg attaatcagg aacctggttt tctgtggaag gtatggacag
    13141 aaagtgaaaa gaaccacgaa gccggtggga tctacctttt cactgatgaa aaaagcgccc
    13201 ttgcctatct ggaaaaacat actgcccgac tgaaaaatct cggcgttgag gaagttgtcg
    13261 ccaaagtttt cgatgtcaat gagccactta gtcaaatcaa tcaggcaaaa ctcgcctgac
    13321 agaatttaat caagggcggt tagcgccctt ttcatccctg tctgaaattt ctcaaattct
    13381 aaaaatctca accaaactta tctgataaca ctaaattcga aagaatgcgt acaggtaagt
    13441 aacaatgaaa aaaattgcta ttgtgggtgc cgggcctacg gggatctaca ccttattctc
    13501 gcttctacag caacaaactc cactttctat ttctatcttc gagcaggctg acgaggccgg
    13561 tgtcgggatg ccatacagtg atgaggaaaa ctcaaaaatg atgctggcaa atattgccag
    13621 tattgaaata ccgccgatta attgtacgta tctcgaatgg ctacaaaagc aagaagccag
    13681 ccatctccag cgttatggcg ttaaaaaaga aaccttgcac gatcgtcagt ttttaccgcg
    13741 aattctgctg ggcgaatatt tccgcgatca atttttacga ttagtagacc aggcacgaca
    13801 gcaaaaattt gcagtggctg tttatgaatc atgccaggtt accgatctgc aaattacaaa
    13861 tgctggcgtc atgctcgcta caaatcagga tttacccagc gagacgtttg atttagcggt
    13921 gattgccacg ggtcacgtct ggcctgatga agaagaagca accagaacgt attttcccag
    13981 cccgtggtca ggcctgatgg aagcaaaggt cgatgcgtgt aacgtgggta ttatgggaac
    14041 atccttgagc ggactggatg cggcaatggc agtggctatt cagcatggtt cgttcattga
    14101 agatgataaa caacacgtcg tttttcaccg cgataacgca agtgaaaagc taaatattac
    14161 gttgatgtcg cgcacgggta ttttacccga agccgatttc tattgcccta ttccctgcga
    14221 gcccttacac atcgtcaccg atcaggcatt aaatgctgag attcaaaaag gtgaagaggg
    14281 ccttttggat cgggtattta gattgatagt agaggaaatc aagtttgctg atccagactg
    14341 gagccaacgc atagccttag agagcctgaa tgtcgattcc tttgctcaag cctggtttgc
    14401 cgagcgcaaa caacgcgacc catttgactg ggcagaaaaa aatctccagg aagtcgaacg
    14461 caataaacga gaaaaacata ctgttccctg gcgttatgtc attctgcgcc tgcatgaagc
    14521 cgtacaggaa attgttcctc atctgaatga acacgaccat aaacggttca gtaaaggcct
    14581 tgccagggtt ttcatcgata attatgcggc aatcccttca gagtctattc gtcgactact
    14641 ggccttacgt gaagcgggga tcattcatat tctcgccctc ggtgaagact acgaaatgga
    14701 aattaatgag tcgcgcaccg tcctgaaaac ggaagacaac agctactcgt ttgacgtttt
    14761 tattgatgcc cgcggacagc gtccgcttaa agtgaaagat attcctttcc ctgggctacg
    14821 cgagcaatta cagaaaacag gggatgaaat ccctgatgtt ggcgaagatt atacgttaca
    14881 gcaacccgaa gatattcgtg gacgcgtagc gttcggcgcg ttgccctggc tgatgcacga
    14941 ccagcctttc gttcagggac ttacggcatg tgcagaaatt ggtgaggcga tggctcgggc
    15001 ggtcgtaaag cctgcatccc gtgctcgtcg gcgtctttcg tttgattaaa ggtaaagcta
    15061 tcagcactca acaaactgat gctgttcatt aagacgatag cggactccgg ttcgaatatt
    15121 gttttcacct tcaatggcga cggcgaagcg aactctttcg ccgtcatgat atgccagtgc
    15181 ggcgcttccc cctagcccga gaataattga gtcgaccacg cccgtgctca ctaccgttga
    15241 attttcaccg ctggcaatga tgtgtacatt gtcgccgctg ttggcaattc tggcgtttgc
    15301 gccaaagctg gcgatttgca ccaggtcacc attactggcg acatgacacc gttcacccaa
    15361 tgtacaaacg cgtactcgca ttccggtatt cgcaatccga ctactatctc cagcgctgct
    15421 tatccgcgaa gaattaccgg ttacagcaac ccgtgcgcgt tcaccggagc tgccaatgtg
    15481 actgttaaag ccgacacttc caatccgcac gctataaccc atactggcaa tttgcgcggc
    15541 atacccagcg gttgcgattc gggcgttatc ctcacaacag ccgatgcgag catgatcgtc
    15601 gctgcgggca aactgactgc aaaatggtat ctctccgggg tctgtggcca gtttttgcat
    15661 agaggaaact tcctgatgcg cgaagttttc atccgccagc cattttttcc acgcatactc
    15721 aaccagactc tccgcccagt ccgtatatcc ttcctcaatc agtgcatgac gaatatcggc
    15781 ataactaccg ccgcgtggga aatggcgcag gaaccagcga tacatcaccg ctccgatacg
    15841 ccattctcgc aggtctgctc gtgtaatgat cattcgttag tgccggtgat agccatcgac
    15901 catgctttta aaggtttcgt gtggcgtacg ccccgtggtg caaagataaa gatgaccgaa
    15961 gataaaaaag aggcttataa atgccagagc aaaatgcgcc tgcaataacc agtatcttac
    16021 gccaggaaac acatctccca cggcttgcgg atagagacac aacagccccg tcaatagtaa
    16081 caacggcagc aatccataca tgacaccaac ataggcgacc tgctgtaagg gattaaattt
    16141 agactgtgtt gttgccggga aaggatgttc ttccccctgc ataatgccaa acaaataaaa
    16201 tcgcgtttgt tttgccgctc gttccagcca cccctgacga cgaatgcgat agtggtgacc
    16261 attatcccca acggcattga tcagcacaaa gccgagccag catgccagta acaaaaatca
    16321 gcaaacttca tgcaccgcaa ccagactttt aaccgcagtt gcgcccacca tcgcaaagtg
    16381 atttatcagc ccactt
//
LOCUS       DADLZM010000092        16264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLZM010000092 DADLZM010000000
VERSION     DADLZM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.093"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            9..746
                     /locus_tag="JNY73_002749"
     CDS             9..746
                     /locus_tag="JNY73_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY8048704.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JNY73_002750"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JNY73_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY8048705.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="JNY73_002751"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="JNY73_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY8048706.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="JNY73_002752"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="JNY73_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY8048707.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..6724
                     /locus_tag="JNY73_002753"
                     /pseudo
     CDS             5041..6724
                     /locus_tag="JNY73_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            6776..7931
                     /locus_tag="JNY73_002754"
                     /pseudo
     CDS             6776..7931
                     /locus_tag="JNY73_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(7897..8019)
                     /locus_tag="JNY73_002755"
     CDS             complement(7897..8019)
                     /locus_tag="JNY73_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048708.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8222..9283
                     /locus_tag="JNY73_002756"
     CDS             8222..9283
                     /locus_tag="JNY73_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY8048709.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLINKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTE"
     gene            9377..9907
                     /locus_tag="JNY73_002757"
                     /pseudo
     CDS             9377..9907
                     /locus_tag="JNY73_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            9945..12317
                     /locus_tag="JNY73_002758"
     CDS             9945..12317
                     /locus_tag="JNY73_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY8048710.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12362..15157
                     /locus_tag="JNY73_002759"
     CDS             12362..15157
                     /locus_tag="JNY73_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY8048711.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETF
                     ESMGLRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVISEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            15519..>16264
                     /locus_tag="JNY73_002760"
     CDS             15519..>16264
                     /locus_tag="JNY73_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8048712.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGG"
BASE COUNT         4735 a         3574 c         3580 g         4375 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
     5221 atcgtactaa ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
     5281 gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa
     5341 gccattgaag ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta
     5401 cgttttacta acggctatgt ggcacacggt gtttccggcc cctcccgtgc cgcaataatg
     5461 accggtcgag ctcccgcccg cgttggtgtc tattccaata ccgatgctca ggatggtatt
     5521 ccgctaacag aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca
     5581 gtaggtaaat ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg
     5641 cgtgactatc atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt
     5701 ggctttgatt actttatggg attccacgct gcaggaacgg catattacaa ctccccttca
     5761 ctgttcaaaa atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat
     5821 gaggcaattg gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg
     5881 gcttataatg ctccgcacct accaaatgat aatcctgcac cggatcaata tcagaagcaa
     5941 tttaataccg gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag
     6001 ggtgtaaaac gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt
     6061 ctctttacct ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa
     6121 aaaggctata agagtcagac ctatcctggc ggtactcacc accccaatgt ttatgtggtg
     6181 gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg atttctaccc
     6241 aacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg atggtgtttc
     6301 cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc tgacctggat
     6361 aacctcttat tctcactggt ttgatgagga aaatattcca ttctgggata attaccacaa
     6421 atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg acttaagcca
     6481 attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag aaaacaatca
     6541 gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg ccgccaatcc
     6601 gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca gccagccacc
     6661 gcttagcgag gtaaatcagg agaagtttaa caatatcaag aaagcactaa gcgaagcgaa
     6721 ataactaaac cttcatgcgg cggatttttc cgccgcctta ttgagcgaga tagcaatgca
     6781 cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact gtttttacct
     6841 tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc tgaaagagtt
     6901 catcaaacaa tatatcgaag cgtctggcaa tcaggtctat tttacctggc aaggcggtga
     6961 acccactctg gctggcctgg attttttccg taaagttatt cactatcaac aacgctatgc
     7021 aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga ataatgaatg
     7081 gtgtgccttt ctcaaagaac atgattttct ggttggtatc tcgatcgatg gcccccagga
     7141 gttacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa aagtgatagc
     7201 agccatcgag cgtctgaaat catatcaagt agagtttaat acgttaaccg tcattaataa
     7261 cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg gcagtaaaca
     7321 tatgcaattt atcgaattgc tagaaaccgg gacgccgaat attgatttca gtggtcatag
     7381 tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg gcaagtttat
     7441 gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt
     7501 tgaaagcttt gtcagccgtt ttttggggaa tgggcataca agttgtattt tccaggagtc
     7561 ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg accattttgt
     7621 ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt
     7681 acaactgaca gcgcaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     7741 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     7801 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     7861 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     7921 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     7981 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     8041 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     8101 ccataaccac tgaaaattaa aaatttctat atatttcata ttgttaaatg atacttaaat
     8161 aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct
     8221 aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa
     8281 gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc
     8341 aatgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt
     8401 caatgcgcta agccagaaag aaaccgataa gctttggcag cttgttcttt ggttccctgc
     8461 cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac
     8521 tattcgctgg cgcgaatggt taacagatta ctaccttaat cgctggtttg cagataaaaa
     8581 ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat
     8641 cgctgaagac attctcttac tgatcaacaa aacgctcagt ctttcatttg gcttcatcca
     8701 gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc
     8761 attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata ccgtcgtgct
     8821 cattgtgatc ggtggaactt tatttaccca taaggtgggt aaacgtattc gtccgcttaa
     8881 tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat cttgtgcagc ataataagca
     8941 ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa
     9001 ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta
     9061 ctggcaaaat atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca
     9121 gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct
     9181 agtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc
     9241 ggttatcgat cgcttgtatg agttccatca actcactgaa tagcgcccta cgaataagcc
     9301 taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc ctgataataa
     9361 gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat tactgaaagg
     9421 ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct ggccgtgatt
     9481 taaaggtgat atttcttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa
     9541 aagcggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc
     9601 gttgagcgaa gtactgcatc aggttggtct ggggaaattg gctgcgcgta ttcatgacca
     9661 cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt
     9721 aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca
     9781 agaggctata cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat
     9841 ggttacacat caacccggtg tctggaacct ggctgatgat atttgtgaca ttagcacggt
     9901 tttataacaa caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta
     9961 ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt
    10021 acatgcattt ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca
    10081 acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg
    10141 atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc
    10201 agggagatat tcgccctgag aaaatctcta ttcacggtgc gtcgccctac cagaatgcct
    10261 atttgattga cggtattagt gcaactaata acctgaaccc agcgaatgag tccgatgcca
    10321 gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg
    10381 acaatgtgac gctttatgac agttttgtgc cggttgaatt tggtcgcttc aatggcgggg
    10441 taattgatgc aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc
    10501 gcactacgcg tttggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta
    10561 atcaaggttc ttcaggaagt acctatttct ctccagattt taaaaagaac ttttatacct
    10621 tgtcgtttaa tcaggagctg gctgataact ttggcgttac cgccggttta tcgcgccgcc
    10681 agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac
    10741 agtataaaaa cgttatcgat actgcattga gcaaatttac ctggtttgcc agcgaccgct
    10801 ttacccacga tttaacctta aaatataccg gctccagccg tgattataat accagcacct
    10861 tcccgcaatc tgatcgcgaa atgggtaata aatcctatgg tctggcatgg gatatggata
    10921 cgcaactcgc atgggccaaa ctacgtacca ccgttggttg ggatcatatt agtgattata
    10981 cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag
    11041 ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca
    11101 aaacacgcct ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctacttcg
    11161 gcgcggaata catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg
    11221 tgattaatgc tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc
    11281 tgggaattga caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt
    11341 taatgcccgg tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc
    11401 gctttatgac ggaatgggat atttttgctg atcaaacctc aatgattacc gccggttata
    11461 accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga
    11521 cggaatcggt atcaggtaat aaaaccctga cgcgttatca gaatttgaaa acgccttata
    11581 acgatgaact ggcaatggga ttgcagcaaa aaatcgataa gaacgttatt gcacgcgcaa
    11641 actatgttta ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
    11701 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
    11761 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
    11821 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
    11881 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
    11941 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
    12001 gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc
    12061 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
    12121 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
    12181 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
    12241 ttgatacaac gaacaccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
    12301 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
    12361 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
    12421 ccccgggcgg ctgattgccg ccgccttaca gcaggatgaa aagttaatta ccgggcaact
    12481 ggacaatggc ttgcgatata tgatttatcc gcatgctcat ccaaaggatc aggtaaattt
    12541 atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca
    12601 ttttgtagaa catatgatgt ttaacggcgc aaaaacatgg ccgggtaata aagtcatcga
    12661 aacatttgag tcaatgggcc tgcgttttgg tctcgatgtt aatgcctata ccagctatga
    12721 cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat
    12781 ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga
    12841 acgtggcgta atttctgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca
    12901 ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct
    12961 gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta
    13021 tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaag aagcgctggc
    13081 gctgataaag gataatttaa gtaagtttcc ggctaacaaa gcagctgaaa atcgcgtctg
    13141 gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt
    13201 gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt
    13261 tgtcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg
    13321 catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc
    13381 acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgcaggatgc
    13441 tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga
    13501 actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc
    13561 tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc
    13621 gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt
    13681 gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca
    13741 aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct
    13801 ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc
    13861 gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa
    13921 tctgacatca ttaacacttt ccaatggtgc caaggttatt ctggcaaaat ccgcgggtga
    13981 agagcaaaag ctacaaatta ttgccgtatc gaataaaggc gatttaagtt tccctgcgca
    14041 gcaaaaatca cttatcgcgc tggcaaataa agcagttagc ggaagcggcg ttggcgaact
    14101 ctcctcttcc agcctgaaac gctggagtgc ggaaaactcg gtaaccatga gcagtaaagt
    14161 cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt
    14221 ccagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct
    14281 acaaaatgct caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc
    14341 ccagctgatg tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca
    14401 gattgcacag tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc
    14461 agcggatatc acgtttgtca ttgtcggtaa tgtcgcagaa gacaaactcg tggcgttaat
    14521 tacgcgttac ttaggctcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt
    14581 aactcgcgcg acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca
    14641 ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc
    14701 gctcgatgct tttaacgtcg cactggcaaa agatctacgc gttaatattc gtgaacaggc
    14761 atctggggca tacagcgttt cttctcgcct ctcggttgat cctcaggcta aagatatcag
    14821 tcatttgctg gcttttactt gtcaaccaga acgacatgat gaacagttaa cgttagcgaa
    14881 tgaagtgatg gttaagcgtt tgactaaggg gatcagtgag caagaactga acgaatacca
    14941 gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac
    15001 tattataaat agtcttattc aatatgacga tcctgcagca tggactgagc aggagcaatt
    15061 gttgaaacaa atgacgatag agaatgttaa cactgccgtt aaacaatatc tttctcatcc
    15121 ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt
    15181 tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa
    15241 ataataacca agtactaata gtgatatttt aatgtctgat ttttacgtga taattcagga
    15301 ggcacagaaa gcgcataaaa ataacaacat aaaacacctt gccaccaccc aagaattctc
    15361 tattgcattg tttttaatga aaaaaatatc attccaacaa tatctcacga taaataacat
    15421 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
    15481 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
    15541 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
    15601 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
    15661 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
    15721 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
    15781 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaacatggtt gccgatctgt
    15841 ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg
    15901 aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg
    15961 caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtgcaaatc tgctggcata
    16021 aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt
    16081 ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc gtggtgccga
    16141 ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac gatgcactgg
    16201 ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc gccagcggtg
    16261 gctt
//
LOCUS       DADLZM010000093        16030 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLZM010000093 DADLZM010000000
VERSION     DADLZM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.094"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(186..872)
                     /gene="kptA"
                     /locus_tag="JNY73_002761"
     CDS             complement(186..872)
                     /gene="kptA"
                     /locus_tag="JNY73_002761"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY8048713.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JNY73_002762"
     CDS             1020..1703
                     /locus_tag="JNY73_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY8048714.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JNY73_002763"
     CDS             1700..2161
                     /locus_tag="JNY73_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048715.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JNY73_002764"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JNY73_002764"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY8048716.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JNY73_002765"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JNY73_002765"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY8048717.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSRAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNY73_002766"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNY73_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY8048718.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /locus_tag="JNY73_002767"
     CDS             complement(4704..4787)
                     /locus_tag="JNY73_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY8048719.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="JNY73_002768"
     CDS             5380..6210
                     /locus_tag="JNY73_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8048720.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYFGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNY73_002769"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNY73_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY8048721.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="JNY73_002770"
     CDS             complement(7339..8799)
                     /locus_tag="JNY73_002770"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY8048722.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGSAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNY73_002771"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNY73_002771"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY8048723.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="JNY73_002772"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="JNY73_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY8048724.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNY73_002773"
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNY73_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY8048725.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNY73_002774"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNY73_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY8048726.1"
                     /translation="MKWCKRGYVLAAMLALASATIQPADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNY73_002775"
     CDS             complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNY73_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY8048727.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="JNY73_002776"
     CDS             complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="JNY73_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY8048728.1"
                     /translation="SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSG
                     SKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRG
                     FAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGS
                     TQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYG
                     GTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNES
                     GTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGK
                     LQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAW
                     QKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED
                     NNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVL
                     AHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVAL
                     DTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSE
                     SSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAE
                     CR"
BASE COUNT         3861 a         4136 c         3923 g         4110 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tgactgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccgg gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattattt tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcactac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcggcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcataacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctggctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
    13741 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
    13801 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
    13861 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
    13921 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
    13981 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
    14041 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
    14101 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
    14161 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
    14221 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
    14281 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
    14341 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
    14401 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
    14461 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
    14521 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
    14581 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
    14641 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
    14701 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
    14761 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
    14821 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
    14881 tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
    14941 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
    15001 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
    15061 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
    15121 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    15181 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    15241 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    15301 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    15361 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    15421 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    15481 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    15541 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    15601 cggcatagat atcattgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    15661 atccattttg tttaatagtg acctgtgcag taccatgagc aataccgtgg atcaccgggg
    15721 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    15781 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    15841 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    15901 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    15961 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    16021 tctgtacact
//
LOCUS       DADLZM010000094        15976 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLZM010000094 DADLZM010000000
VERSION     DADLZM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.095"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            133..840
                     /locus_tag="JNY73_002777"
     CDS             133..840
                     /locus_tag="JNY73_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8048729.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            837..2288
                     /gene="djlC"
                     /locus_tag="JNY73_002778"
     CDS             837..2288
                     /gene="djlC"
                     /locus_tag="JNY73_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY8048730.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIINRMLLAKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2348..4018)
                     /locus_tag="JNY73_002779"
     CDS             complement(2348..4018)
                     /locus_tag="JNY73_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY8048731.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASASDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(4102..5037)
                     /gene="rihA"
                     /locus_tag="JNY73_002780"
     CDS             complement(4102..5037)
                     /gene="rihA"
                     /locus_tag="JNY73_002780"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY8048732.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(5155..5880)
                     /gene="gltL"
                     /locus_tag="JNY73_002781"
     CDS             complement(5155..5880)
                     /gene="gltL"
                     /locus_tag="JNY73_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY8048733.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5880..6554)
                     /gene="gltK"
                     /locus_tag="JNY73_002782"
     CDS             complement(5880..6554)
                     /gene="gltK"
                     /locus_tag="JNY73_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY8048734.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6554..7294)
                     /gene="gltJ"
                     /locus_tag="JNY73_002783"
     CDS             complement(6554..7294)
                     /gene="gltJ"
                     /locus_tag="JNY73_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY8048735.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTASRVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7464..8371)
                     /gene="gltI"
                     /locus_tag="JNY73_002784"
                     /pseudo
     CDS             complement(7464..8371)
                     /gene="gltI"
                     /locus_tag="JNY73_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            complement(8768..10306)
                     /gene="lnt"
                     /locus_tag="JNY73_002785"
     CDS             complement(8768..10306)
                     /gene="lnt"
                     /locus_tag="JNY73_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY8048736.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10331..11209)
                     /gene="corC"
                     /locus_tag="JNY73_002786"
     CDS             complement(10331..11209)
                     /gene="corC"
                     /locus_tag="JNY73_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY8048737.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11299..11766)
                     /gene="ybeY"
                     /locus_tag="JNY73_002787"
     CDS             complement(11299..11766)
                     /gene="ybeY"
                     /locus_tag="JNY73_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY8048738.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     MVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11763..12842)
                     /locus_tag="JNY73_002788"
     CDS             complement(11763..12842)
                     /locus_tag="JNY73_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY8048739.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AREEAEQKRKAALAAERKREEQEQK"
     gene            complement(12956..14380)
                     /gene="miaB"
                     /locus_tag="JNY73_002789"
     CDS             complement(12956..14380)
                     /gene="miaB"
                     /locus_tag="JNY73_002789"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY8048740.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14526..15701
                     /gene="ubiF"
                     /locus_tag="JNY73_002790"
     CDS             14526..15701
                     /gene="ubiF"
                     /locus_tag="JNY73_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY8048741.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(15855..15929)
                     /locus_tag="JNY73_002791"
     tRNA            complement(15855..15929)
                     /locus_tag="JNY73_002791"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15895..15897),aa:Gln,seq:ctg)
BASE COUNT         3894 a         4060 c         4134 g         3888 t
ORIGIN      
        1 ttgcgaatat atacattaat tttttattca tgtgtgaatg cttcgatttt ctttttattt
       61 tgaaagcgtc aagtttatac tcatcaataa taaaaaagta gtcagttaat gcacttaagg
      121 gattattaaa aaatgaataa ggaagaacaa tatcttcttt ttgcgctttc tgcgcccatg
      181 gaaatactta atcagagttg caaacccgcg catgactcgc caaaaatgta taccgggata
      241 aaagaattcg atctctcatc atcctgggga ataaataatc gtgatgattt aattcagact
      301 atttatcaga tgactgatga tggtcatgcc aatgatttag ctggcttata tctgacatgg
      361 caacgctctt cccctgaaga gtggaaagca ttaattgctg gtggctcgga gagagggttg
      421 atttataccc aattcgttgc ccaaaccgct atgtgctgtg gagaaggggg aatcaaagcc
      481 tgggattatg ttcgaatggg gtttctgtca cgggccggtg tgctcaacaa ctggctgacg
      541 gaagaagaga gtttatggct gcaatcgcgc gtctatgcac gagctcacca ttactatcac
      601 agctggatgc actatttttc tgcctattca ctagggcgtc tctactggca atcttctcag
      661 tgcgaagaca acgcttcgct acgcgaggcg ctaaccctgt acaaatacga tagcgccggg
      721 agtcggatgt tcgaagagct ggcagcggga agcgaccgtt tctatgccac gcttccctgg
      781 cagccattaa ctgtccagcc tgaatgcccg gtaacgctta aggatgtgag cgacctatga
      841 aaacatgttg gcaaatcctc gaaattgaaa gcacgacgca aatagacatt atccgccagg
      901 cttatcttgc tcgcttaccg ttgtgtcatc ccgaaaccga tccgcagggg tttaaagcat
      961 tacgccaggc ctatgaagag gcgctgcgac tggcggtaaa tcctgtcgag gaagcagatg
     1021 atgaagaaaa agatgccacc gctgaacatg aaatactacg tgcattcagg acattactgg
     1081 attcagaaag tgatcgtttt cagccctccg cttggcagaa atttattcag caattaaata
     1141 cctggaacat ggaggatgtc gatcaattac gctggccgct gtgtgcaatc gctatagaag
     1201 cgcgatatct gtcattaaat tgtgcttctt tgttggcaga gcgtttgaac tggcattcgt
     1261 ttaatgacag cgaaggaatg gatgaggaag aaagggaggc ttttcttgag gccattcagg
     1321 ctggtgattg tttcgatttc cttagccttc tggaatatcc cgtcgcgtta cagaaccaga
     1381 ctgttgaata ttacttcgcg ctggaacgtt gctgccgtta ccatcctgac tatgtcactg
     1441 cgtttttggc gatggaagga ccgtggttca ttcctgatga tgcaaagtta catcgcaaac
     1501 tgttgcgctg gtacagctcg gtgcaaacag gtatggtgga actcattcct gtagccaaac
     1561 agtggcaagc ggaagaacca gaaagcgaag atgcccggta ttaccagtgt gcacaacgtt
     1621 tgtactgcgg cgagggggaa agcctgcttg ctgatctcgg cgcgtactgg gaaagttacc
     1681 catctacaca agctgataat ttgttgttgc agtggagcaa gcgtcattgc ccggattatt
     1741 tcgcgttatt agtgatggtt atcgaagcgc ggagcatggt agatgcgcaa ggtcaaccgc
     1801 tgaaatatgt tcctggtgag agcgcccgga cgcggctgtt atgggcggaa attttacata
     1861 gcggaaaatt atcgccgtta ggtcaatcgt ttattgagtc gttattcttc aaacgcaaag
     1921 catgggcgtg gtggaaatcg agagtcggta gcgagacaga agaagattca ccgttactgg
     1981 atttgtatcg ggtagcggaa caggtagtac ttgaagcgtt tccgaaacaa gagatgctgg
     2041 cccgtcttaa tacaaggctg gaaggcggag atgctcatcc attagaggcc attatcaacc
     2101 ggatgctgtt ggcgaaagta aaactcgagc cggaggatga agatgtcgat gagccaacac
     2161 ctgaaaacca tgaagaaaaa aatgatgagg gtgaaaaacc acagagcatt accagcatta
     2221 tcaaaatcag tttaacggtg ctggttatag gttatgctct cggcaaaatc gcgatgttgt
     2281 ttagttgacg atgtgttttt tgcgccgatg ccggatgcag catttacgcc gcatccggca
     2341 gccgtgatta tgaggtctca atggctaaat aaacgctcgc ccgccgcctg acctcatcga
     2401 cgcgggccat atcgtttgac tgcagcgccg catcaaaatc ggtgatcacc cggccaatct
     2461 cttctcgctc gtcacccagc gcccgcgccc acaactcttc cagtttggct ttaaaggtgc
     2521 gattgatgag catatcgcgc ggataaattt tcaatgcgga taaccgcgtc cgactctctt
     2581 caatctgctg cgcgctcaat gttaccgggc tgtggttaat cactctggac ttaacgctgc
     2641 cgtcttccag aagcacgtca acttcaagca acccgttgat atcataacta aagcgaatat
     2701 caatcgactg ataagccccg gttttcttca acggcacatc gaaggattcc accagaatgt
     2761 tgtttttaac tttgtggttt tctccctgat agacgttaac cgtaattgaa tcctgttccg
     2821 ggtgcatggt tgaataagtt tctacacgcg acacgggcac agtggtgttt cgttcaataa
     2881 tcggcgagaa aatgccggaa acgccctggc ggttaacctc aacgcccaac gagtaagggc
     2941 aaatatcagt gaggatcact tcttcaatat cttcactgcg taagcggcag gcggcctgga
     3001 ttgctgcgcc cagcgcgaca atggtgctcg gatcgtaact ttgatacggt aatttgccaa
     3061 acagacgcac ggcgattcgc tgcaccagcg gcatttgtga cgcgccgcca accagcacca
     3121 gactgtcgat ttgactcggc ttcagacgcg catcgcgcaa cgcctgttca atcggcactc
     3181 gcaagcgatt gagcagcggc aaccacaaat cttccagttc gttctcgtaa aattcgcatt
     3241 cccgcgtttc atcctggtac tgccagcgaa tgtgcattgg cgattgattg ctacattttg
     3301 ccgcttccac acaggcgtac aaggctgcca gttcactctc gttaagcgtg gtccttgtga
     3361 catccgcgcg ctttaaaacg tcatcgacca acatatgggt aaaatcttcg ccaccgagga
     3421 agttgtcgct agcggaggcg tgaacttcaa tcaccggcgt ggcgtactca agcaccgtaa
     3481 cgtcaaacgt gccgccaccg agatcaaaca ccagcgaacg ggtattttgt tgggtatgca
     3541 ggccatacgc catcgcagct gctgtgggtt cattaattaa gcgtaccgca tttaacccgg
     3601 ctaagtccgc tgctaaacgg gtatgcttgc gttgtttatc gctgaaataa gccggaacgg
     3661 agatcaccac atctttaatc ggacgttgca gaaattcttc ggcatcttct tttaatgagc
     3721 gtaataccaa agaggacagt tctggcgcgt taaatgtgtc gctgcctaac cgccagttgg
     3781 tattactgcc cattgcacgt ttaaataacg ctgccgtttt atccggatgc gaagtacgcc
     3841 gtgatacagc cggttttccg actaaaatat gattattttc atccatgcta attatggatg
     3901 gtgttaaata ttcaccgaac ttatttggaa ttaattgcgc ggcaccgtct ttccagacgg
     3961 caattaaact attggtagtg ccgagatcga tacctatggc gagttctgca ttatccattg
     4021 catttatatc ttgaaagaaa aagagataaa tagattaaga ccacccaggt aacgcgtggt
     4081 gatcttaatc aatgacgagt gttaagcgta aaatttcaga cgatcagcca gtaaatcaac
     4141 aaagccctga cgatcaacat cgacaattac agtggcattc ggtttattgc cagtcagata
     4201 ataataatca acaaccgtca tacccagggt atatttcccc tgtgtttcca cgccaaccca
     4261 gcgctcaaca gtggtaaaca actcgggttt caacagccag gcgatggtgc atgggtcatg
     4321 cagcggtgcg ccgacaaagc cccatttttc gtctttatga tattcgagga agaaatccag
     4381 cagttcggca acaatggttg aaacagggtt gccaatcgcg cggaaacgct cggtgtcttc
     4441 aacgtggatt tgcgctttat gagtaacatc cagaccggcc atcaccaccg ggatccctga
     4501 ctggaagaca atttctgccg cttccgggtc aacgtaaata ttaaattcag ccgcaggcgt
     4561 ccagttacca agccccattg cgccgcccat aatcacgata cgggcaattt tgctatgcag
     4621 ttccggatgg ctattgagca gcaaggcaac gttagtttgc ggtccggtag aaacaatcgt
     4681 gacaggttcc gcactttcgc gcagcgtttt cgccatcagc tccaccgccg tacagttttg
     4741 cggtgcgaac gtcggttccg gtaatgccgg gccgtcgaga ccgctttcgc cgtgcacatt
     4801 gtccgcgata atcaactcac gcattaacgg ttttaccgcg ccgcctgcca ccggaatatc
     4861 ggtgcgatta agcaaggtca gcatacgcag aacattgcgt aaggtttttt ctggtgtctg
     4921 gtttccggcg gaagacgtaa ttgctttgac atcaagctct ggtgaggcga gggcgagaac
     4981 tattgcgata gcgtcatcat gacctgggtc gcaatctaac agaattggca gtgccattgt
     5041 tgctccttgt tgtgtgcttc tttgcgacaa gggtaacgcc aggatgtaac agatacgagg
     5101 ggcgaaacga taaagcgtga gatggcgcgc aattgggtat gcgcgccaga gtgattaatg
     5161 caggattttc gcgaggaagt cttttgcgcg gtccgatttc ggatcatcga agaaagcgtc
     5221 tttcggcgag tcttcgacaa ttttaccctc gtccataaag atcacccgat tcgccacttt
     5281 gcgggcaaag cccatttcgt gggtcaccac catcatggtc attccttcgt tcgccagttc
     5341 caccatcacg tccagcactt cgttgatcat ctccggatcc agcgccgatg tcggttcgtc
     5401 gaacagcatc gcaataggag ccatacacaa tgcgcgagcg attgccacac gctgctgctg
     5461 accgccggaa agctgcgccg gaaatttatt ggcatgtgcg gaaagtccca cgcgctccag
     5521 caatttcaat gctttttcac gagcgggcgc tttatcgcgt ttaagcactt tcacctgcgc
     5581 cagggtcagg ttttcgataa tcgacagatg agggaacagc tcgaaatgct ggaataccat
     5641 cccgacgcgg gagcgcagct ttgccagatc ggttttcttg tcgttaacca cgataccatc
     5701 gacggtgatt tcaccttgct gcactggttc gaggccgttg acggttttaa tcagcgttga
     5761 tttgccggaa ccagacgggc cgcaaaccac caccacttcg ccttttttca cttcggttga
     5821 gcagtcggtc agcacctgaa agtgaccata ccattttgaa acatttttca gggtaatcat
     5881 tatgctgtcc ttcttttcaa gtagctgacc aacaacgacg cgctaaggct aataacgaaa
     5941 taaacaaatc cggcaaacag gatcatctcg acctgcgtac catcacgctc accaatggtt
     6001 gaggcggtac ggaagaaatc ggccagagat aacacataca ccagcgaggt atcctggaac
     6061 agtacgatgc cctgagtgag cagcagcggc accatcgcgc ggaaagcctg cggcagaata
     6121 atcagtttca tcgactgcca gtgagtcatc cccaacgcca gcgcggcgct cgactgacca
     6181 cgggaaatac tttgaatacc ggcacggata atctctgaat agtaggccgc ttcaaacatc
     6241 gaaaacgcca ccatcgccga aattaaacga atatcatttt ttggcgataa tcccagcaca
     6301 ttttgcagaa atcccggcac aatcaagtaa aaccacagca aaaccatgac taaaggaatc
     6361 gagcggaata cgttaacgta ggctttggca aaccacgaaa caggcgcgaa gctggacaag
     6421 cgcatcaccg ccagcatggt accccacaaa ataccaatca cgactgccgt gacggtgatt
     6481 ttcagggtga tcaccagccc gtcgagcaga tatggcaggg aaggaacaat ggaactccag
     6541 tcaaactcgt acattatttg ccccccatgt tgccgggcag gcgaacttta cgttcaacca
     6601 gcgtcatcac cagcatgata aaagcgttaa tcaacacata cgtaagcgtg atggcggtaa
     6661 acgactccca ggcgtgagcc gagtagtcga gcaatttacc cgcctgcgcc gccatatcca
     6721 ccagaccgat ggtcgaggcg atggcggagt ttttcaccag gttcatcatc tcggaagtca
     6781 tcggcgggac gataacgcga taagcattag gcagcagtac gtatcgataa gtctgcggta
     6841 gcgtcaggcc catcgccagc gcggcattct tctgcccacg cggtagcgac tgaatagcgg
     6901 cgcgtacctg ttcgcaaaca cgggaggcgg taaacagccc caggcagagc atggatgaaa
     6961 ggaaaaactg aatattggga tccagctcgg ccttaaacca catgccgatt ttctccggca
     7021 gcagctccgg gatcaccaga taccaggtaa agaattgcac aatcaacggc acgttgcgga
     7081 acagttcgac atacaacgta cccagaccgg agagaaaacg gtttggaacg gtacgtaaaa
     7141 tgccgaaaaa tgaaccgacg aggaaagcga taatccaggc gcagatcgac aaagcgatcg
     7201 tcacctgaaa accactccag acccaaccaa gataggtggt attgccgaac ggggcttgtt
     7261 gtaaaaaaat accccagttc cagtctatag acataaatct actccagaaa aaagagggta
     7321 gcagcgttaa ctgctaccct cgaagattgt tacccagcgt attgcggttt tcaggcccga
     7381 tggggaacga ccatcaggcg tatagtctgt ccgtgctacg taacaatcga gagggctgga
     7441 atttccgccc ctggttcttg taattagttc agtgccttgt cattcggttc tttgaacagt
     7501 gctttcattt cgtctgacag ttcgaaattc atgttcaggt ttttcggcgg aattggattt
     7561 ttgaaccatt tatcaaacca tttttccgct tcaccggagg tctgcacctg agcgatagtg
     7621 tcatccatca gctttttgaa ctgcggatcg tctttacgca acatgcaacc gtaggcttcc
     7681 tgagactgcg gcttgccgac gatatcccag ttgtctggtt tcttcgcttt cgcacgttca
     7741 ccggccagca gagcgtcatc catcataaag gcaacggcac gaccgctttc cagggtgcgg
     7801 aaagagtcac cgtgatcttt ggcgctgatg atgcgcatat tcattttttg ctcttcattc
     7861 agtttgttga gcaaaacttc agaggtagtg ccggaagtga cgactacggc tttgcctttc
     7921 aggtcggcaa aatctttgat atcgccgccc tttttggtca acaggcgcgt accgaccacg
     7981 aaaatagtgt cagagaaagc cgcctgtttt tggcgttcga cgttgttggt ggtagaacca
     8041 cattcaaaat cgaaagtgcc gttttgcagc agtggaatac ggtttgtgag gtaatcggaa
     8101 tcagttttac ctgcaagtcc ggtttgttga gtttcttttt cactgcttca acaatggcgt
     8161 tggagtaatc ctgcgagtaa cccaccactt tttgctgatt gtcgtaataa gagaaaggta
     8221 ctgaagattc acggtgaccg acgacaatca caccgttttt ggcgattttg tccagcgtac
     8281 tgcctgctgc cggggcggcg tcatctgcct gtgccagtcc tgcggaaagc gccagggcga
     8341 ggattgctgt ggcaggttta cgtaattgca tatccaactc ctttatcttc tgcgttaaga
     8401 acgcatggat acccgttgtg agtgtttgtg ttgttatcgt ctgcaacttt attgtgcagt
     8461 gttgtgcctg ttaggtaaca tttagtttgg ctaaatgtaa agatattgat gttttattgt
     8521 ttgtttttgc gaggtgcacc gcaccattct gaagcaaaat tcttaaaatg cactcttttg
     8581 gtgctaccgg tggattgctg tggtgcaacc gggttgtact gatgctggtt caagctttgc
     8641 cttgtataac aaagcaatag atgtgccaaa gttggatagg agaatattgt tatccggata
     8701 atgcactgat gccgcatccg gtgagcgtgc cgaaatatgg gatgtattcc ggcacgataa
     8761 gaagggatta tttacgtcgc tgacgcagac tcatcaacac agcagcaaaa ccaaacagtg
     8821 ccgtcagcac ccacagcggc cagttgccgg tacgtgcgta tggtgtgagt ccggtggtcg
     8881 gcgtcacgtt agtggttaac acctcgcggg tgaactgcgg gatcatcgcc tgaatctcac
     8941 cctgcgggcc aatcaccgcc gtaatgccgt tgttggtgct gcgcaacagt gggcgcgcca
     9001 gctccagcgc acgcattcgc gccatctgga agtgttgcca tggaccaata gatttaccaa
     9061 accacgcatc gttggagata gtcagcaggt agtcggtatc cgggcggaag ttatcgcgca
     9121 cttgctcgcc gagaatgatc tcgtagcaaa tagccgcagt aagctcaata ccatttgccg
     9181 acagcggcgg ctggatatat ggcccacggc tgaacgacga catcggcaga tcaaagaacg
     9241 gtgctaacgg acgcagaatc gactccagcg ggacaaactc gccaaacggc accagatggt
     9301 ttttgttata gcgatcggct gattcgtagc tgtacggtgc acctttaccc agcgtgatga
     9361 tggtgttgta ggtatcgtag cggttctgct tattgagacg cgcgtcgaca atcccggtta
     9421 ccagcgagct acctttatca cgcaactcac cgtccagtgc tttgaggaac ggttgctgat
     9481 taatttccag atcggttatc gccgactccg gccagataat caacgatgat ttgcccatca
     9541 gcggtgccgt tgcgttgtag taaatcttta gcgtattaag aagctggcct tcgtcccatt
     9601 tcagcgattg cggaatatcg ccctgaacca tcgaaacctg aatggttttc tccggttgcg
     9661 gggtaaacca ttggatgtaa cgcagcggga agggtagggc aaacagcacg acggccacca
     9721 ccagcggacg ccagttgcgt ttgaccaacg ccagtgccag caggccacta accatcatca
     9781 gcaggaagtt aatggcttcc acgcccatta gcggtgccag cccttttaac gggccatcaa
     9841 tctggctata gccgaactgt aaccacggga agccagtcag cacccaaccg cgcagaaact
     9901 cggtcacttg ccagagagca ggggcggcaa tcgccacgcg cagccaggtg gttttcggcc
     9961 acagacgcga cagcacgcca gcaaacagtc cggtatacag cgacaaatac gccgccagca
    10021 gcaccaccag gaagatgtta accgggccag gcatcccgcc aaaggtcgcg atgctgacat
    10081 agacccagtt aataccgctg ccaaagaggc caaatcccca gcaaaagcca atagcggcag
    10141 actggagtgg gcgacggtta aaggtcaacg cctgaagccc catcagcgaa ataatcgccg
    10201 caggccagac gtcgtaagga gagaaggcca gcgttccgca ggcaccgaat aataacgcca
    10261 gcagcaggcg aatgcgctgg cgttcaatta atgaggcaaa agccatgtag ttatctatcc
    10321 agtttcggtt ttattcatcc agctttggct ggggtgagtc atccgggatt ttgacatgaa
    10381 cctgaataat acgccgacta tcggccatcg ccactttgaa ctggtaaccg tcgatgtcga
    10441 tagtttcgcc acgcgccgga agatgcccaa acgcctgcat caccagacca ccgatagtgt
    10501 cgacttcttc atcgctaaag tgggtgccga acgcttcgtt gaagtcttca atggaagcca
    10561 gtgcgcgcac ggtccaggta tgacgactca gctgacggaa gtcgatatca tcttcttcgt
    10621 catactcgtc ttcaatctca ccaacaatca gttccaggat gtcttcaatg gtcaccagac
    10681 cggaaacccc accgaattcg tcaataacga tcgccatgtg gtaacgctga gagcgaaact
    10741 ctttcagcat ccggtctacg cgcttacttt caggaacgac aaccgcctga cgtaacactt
    10801 tgtccatgcc gaaggcttca gcatcgctgc gcataaacgg cagcaagtct ttcgccatca
    10861 gaatcccttc aatgtgatct ttgtcttcgc taatcaccgg gaaacgtgag tgggcggact
    10921 cgatgatgac atcaaggcat tcgtccagcg tctggttgcg tttcagggta atcatctggg
    10981 agcgggggat catgatgtcg cgaacgcgtt ggtctgcgat gtccatcacc ccttcgagca
    11041 tatcgcgcgt atcttcgtcg ataaggtcgt tctgcccgga atcacggatc agcgccagca
    11101 gttcgtcacg gtttttcggt tcaccgtgga aaagttggct gagtaacagg gagaaaaatc
    11161 ccttcttgtt gcttatcgtg tcactactgt gtgaattgtc gtcgctcatg gcgtcgtatg
    11221 ggttctcatg ttagttaatc aaaacgccgt cgttaatcac caacggcggg gacgtctgcc
    11281 agtcaaatgc ctggcaaatt attctttctc ggcaatgtac ggatcctcat agcccagagc
    11341 aagcataatc tctgtttcga gggcttccat ttcttctgct tcgtcatctt caatgtgatc
    11401 gtaacctaac aaatgcagac tgccgtgcac caccatatgc gcccagtgcg cctccagtgg
    11461 tttgccttgc tcctgagctt ccttctcaac cacctgacgg cagataacca gatcgcccag
    11521 tagcgacatc tccatgccag gcggcacttc aaacgggaag gagagcacgt tggtcggctt
    11581 atccttaccg cggtaggtca gattcagact gtggctttcg gcggtatcga ccatgcgaat
    11641 agtcacttcc gactcttcct gaaactgcgg gatcaccgca ttcagccatg tttgaaactg
    11701 gctctcttcc ggtaacccgg aattatcttc acatgccagt tgtaaatcga ggatcacctg
    11761 actcattttt gttcctgttc ttcgcgcttg cgttctgctg ccagcgccgc tttacgtttt
    11821 tgttctgctt cttccctggc ttcataagcg ttaacgatac gcgccaccac cgggtgacga
    11881 accacgtctt cgctgtggaa gaagttaaag ctgatctctt cgacatcggc cagcacttca
    11941 atggcatgac gtaagcctga tttagtatta cgcggcaggt cgatctgtgt gacgtcgccg
    12001 gtgataaccg cttttgagtt aaaaccgata cgggtcagga acatcttcat ctgttcgatg
    12061 gtagtgttct ggctctcatc gagaatgata aacgcgtcgt tcagcgtacg accacgcata
    12121 taggccagcg gcgcgacttc aataacgttg cgctcaatca gtttctcgac tttctcaaag
    12181 cccagcattt caaacagcgc gtcgtacagt gggcgcagat acgggtctac tttctggctt
    12241 aaatcgccag gcaggaagcc cagtttctca ccggcttcga ccgccggacg agtcagcaga
    12301 atacggcgaa tttcctgacg ctccagggca tcaactgccg cagccactgc caggtaggtt
    12361 ttacccgtac ccgccgggcc aacgccgaag gtaatgtcat ggtcgagaat attggcgatg
    12421 tactgcgcct ggtttggcgt gcgcggctta attacgccgc gtttagtttt gatattgacc
    12481 gctttgccgt actccggcac gctctccgcg ctttgctcca gtacccgcgc ttctttaatc
    12541 gcaaggtgga tctgttccgg ttcgatatcc tgaatctgac cgcgcatcgg ggcagtatcg
    12601 acatacaggc tacgcagaat gtctgccgca gcggtgacgc aaatcggacg gccggtcagt
    12661 ttaaagtggt tatcgcggcg attgatctcg atgccgagac ggcgttcgag ctgcttgatg
    12721 ttgtcatcaa acgggccgca caggctcaac agacgcgcat tgtctgctgg ctccagggtg
    12781 atttcgcgag tgtctatgtt caaaccgttc ctcttatctg tatgccgccg gaagctgaac
    12841 attcaccggc ctataaggaa attattcacg ccacaggaaa aaggcgcaag cattgcgata
    12901 aagatgggga taaagagaga aaaaacaagg cccaccggaa cggcaggcct gagaattacg
    12961 gctgataata acccacgcca aggtcgtttt ctttgcgggt gcgggcaatc accgactccg
    13021 gtgtttctgc catgcgcaga cccatttcat cttcagttcg caccacttta ccgcgcagag
    13081 agttcgggta gacgtcggta atttctacat cgacgaattt accgatcata tccggtgtgc
    13141 cctcgaagtt gaccacgcgg ttattttcgg tacgcccgga aagctccatg atgctcttac
    13201 gcgaagtccc ttctaccaga atacgctggg tggtgccgag catccgacgg ctccatgcca
    13261 tcgcttgctg attgatacgt tcttgcagaa tatacagacg ctgcttcttc tcttcttccg
    13321 gaacatcatc aaccatatcg gcggcaggcg ttcctggacg tgcagagaag ataaagctgt
    13381 agctcatgtc gaaattgacg tcggcaatca gcttcatcgt tttctcgaag tcttcggtgg
    13441 tttcgccagg gaagccaaca atgaagtcag aactgatctg aatatccgga cgcgccgcac
    13501 gcagtttacg gatgatcgct ttgtactcca gcgccgtatg ggtacgtccc atcaggttca
    13561 gaatgcgatc ggaaccgctc tgtaccggca gatgcaggaa gctcaccagc tcaggcgtgt
    13621 cgcggtacac ttcgatgata tcgtcggtga attcgatcgg atggctggtg gtaaagcgaa
    13681 tacgatcgat cccgtcgatc gcagcaacca gacgcaacag atcggcaaac gatccggtgg
    13741 tgccgtcgta gttctcacca cgccaggcat tcacattctg accgagcagg ttgacttcac
    13801 gcacgccctg agccgcaagc tgggcaatct caaacagaat atcgtcagac ggacggctta
    13861 cctcttcacc acgggtgtaa ggcaccacgc agtaggtgca atatttattg cagccttcca
    13921 tgatggagac aaacgcggtc ggcccttcgg cgcgcggttc cggcagacgg tcaaacttct
    13981 cgatttccgg gaagctgata tctacaaccg ggctgcggtc gccgcgcacg gagttgatca
    14041 tctccggcag acggtgcagc gtttgcggcc caaaaataat atcgacatag tgggcgcgct
    14101 ggcgaatgtg ctcgccttct tgcgatgcca cgcagccacc gacgccgata atcaggtctg
    14161 gattcttctc ttttaacagt ttccagcgac ccaactgatg gaagactttt tcctgagcct
    14221 tctcgcggat tgagcaggtg ttcagcagta gcacatccgc ttcttccgcc acgtcggtca
    14281 gttgatagcc gtgggtggca tccagcagat cggccatctt cgatgaatcg tactcgttca
    14341 tctgacagcc ccaggtttta atatggagtt ttttggtcat cgacttgctc ttgcgaaata
    14401 gtagccagga atgcagggcg catagtgtaa tgctttgctg ccgttgtgac cagtatgagc
    14461 gttatcagcc cttgggggta aaaatcctgt aaacttaaag catattgcta acaggatgat
    14521 tgaccatgac aaatcaacca acggaaattg ccattgtcgg cggaggaatg gtcggcggcg
    14581 cactggcgct ggggctggca cagcacggat tttcggtaac ggtgatcgag cacgcagaac
    14641 ctgcaccgtt tgtcgctgac agccaaccgg acgtgcggat ctcggcgatc agcgcggctt
    14701 cggtatcatt gcttaaaggg gtaggggtct gggatgcagt acaggctatg cgttgccatc
    14761 cttaccgcag actggaaacc tgggagtggg aaacggcaca tgtggtattt gacgcagccg
    14821 aattaaagtt gccgttgctt ggctacatgg tggaaaacac agtcctgcaa caggcgttgt
    14881 ggcaggcgct ggaagcgcat ccgaaagtaa cgttacgtgt gccaacctcg ctgattgcat
    14941 tacatcgaga taatgatctt caggagctgg aactgaaggg cggtgaaacg atccgcgcga
    15001 aactggtgat tggtgccgac ggcgcaaatt ctcaggtgcg gcagatggcg ggaattggcg
    15061 ttcatgcctg gcagtatgcg cagtcgtgca tgttgattag cgtacagtgt gagaacgatc
    15121 ccggcgacag cacctggcag caatttaccc cggacggacc gcgtgcgttt ctgccgttgt
    15181 ttgataactg ggcatcgctg gtgtggtatg actctccggc gcgtattcgc cagttgcaga
    15241 atatgaatat ggcgcaactc caggcggaaa tcgcgaagca tttcccgtcg cgtctgggtt
    15301 acgttacacc gcttgccgct ggtgcgtttc cgctgacgcg ccgacatgcg ttgcagtatg
    15361 tgcagccggg gcttgcgctg gtgggcgatg ccgcgcatac catccatccg ctggcggggc
    15421 agggggtgaa tcttggttat cgtgatgtcg atgccctgat tgatgttctg gtgaatgccc
    15481 gcagctacgg tgaagcgtgg gccagttatc ctgtcctcaa gcgttaccag atgcggcgca
    15541 tggcggataa cttcattatg caaagtggta tggatctgtt ttatgccgga ttcagtaata
    15601 atctgccgcc gctgcgtttt gtgcgtaatc tcgggttaat ggcggcagag cgtgctggcg
    15661 tgttgaaacg tcaggcgctg aaatatgcgt tagggttgta gccttacaac attgccggat
    15721 gcgtgccaac cgtaggtcgg ataagacgcg gcagcgtcgc atccgacatt gaaggataag
    15781 acgtgtcaac atcgcattcg acattgaatg aacgcagaaa agcaaaaagc tcgccgaagc
    15841 gagctttttt aatgtggctg gggtacgagg attcgaacct cggaatgccg gaatcagaat
    15901 ccggtgcctt accgcttggc gataccccaa caaattggtt tttgaatttg ccgaacgtat
    15961 tcgacacatt cagaat
//
LOCUS       DADLZM010000095        15952 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLZM010000095 DADLZM010000000
VERSION     DADLZM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.096"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNY73_002792"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNY73_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY8048742.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JNY73_002793"
     CDS             complement(998..2020)
                     /locus_tag="JNY73_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8048743.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JNY73_002794"
     CDS             complement(2034..3536)
                     /locus_tag="JNY73_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8048744.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNY73_002795"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNY73_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="JNY73_002796"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="JNY73_002796"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY8048745.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="JNY73_002797"
     CDS             complement(5853..6194)
                     /locus_tag="JNY73_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY8048746.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNY73_002798"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNY73_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY8048747.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNY73_002799"
     CDS             complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNY73_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY8048748.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11912..12550
                     /gene="msrA"
                     /locus_tag="JNY73_002800"
     CDS             11912..12550
                     /gene="msrA"
                     /locus_tag="JNY73_002800"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY8048749.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12873..14216
                     /locus_tag="JNY73_002801"
     CDS             12873..14216
                     /locus_tag="JNY73_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY8048750.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14278..14484)
                     /locus_tag="JNY73_002802"
     CDS             complement(14278..14484)
                     /locus_tag="JNY73_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY8048751.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKMHLSVMSEVNRQVMRLQTEMA"
     gene            14809..15363
                     /locus_tag="JNY73_002803"
     CDS             14809..15363
                     /locus_tag="JNY73_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY8048752.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15426..>15952)
                     /locus_tag="JNY73_002804"
     CDS             complement(15426..>15952)
                     /locus_tag="JNY73_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048753.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3796 a         4371 c         3921 g         3864 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agcagacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtcctt tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgtg ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agctaccacc accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa
    11041 atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    11101 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    11161 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    11221 ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc
    11281 ataatcgccc tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata
    11341 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    11401 cacacctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    11461 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc
    11521 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    11581 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    11641 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    11701 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    11761 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    11821 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    11881 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    11941 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    12001 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    12061 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc
    12121 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    12181 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    12241 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    12301 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    12361 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat
    12421 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    12481 tctgcataaa aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc
    12541 ggaagcatag cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc
    12601 tgatgcggct taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag
    12661 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    12721 attttaaccc tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg
    12781 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt
    12841 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    12901 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca
    12961 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    13021 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    13081 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    13141 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    13201 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    13261 aagcggtggc tttgcgtatc atcaatccga tgcgcttctg cctgtacgtt tgcaccccgc
    13321 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    13381 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    13441 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    13501 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    13561 atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    13621 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    13681 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    13741 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    13801 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga
    13861 ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    13921 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    13981 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    14041 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    14101 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    14161 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    14221 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    14281 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    14341 catcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    14401 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    14461 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    14521 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    14581 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    14641 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    14701 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    14761 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    14821 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    14881 atcggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    14941 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg
    15001 caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    15061 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    15121 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    15181 ccctggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    15241 gaagaaagtt ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac
    15301 ggggcgctca caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa
    15361 taatggcaat aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag
    15421 gggaattaag tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt
    15481 ttaaacgcct caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta
    15541 tctgcggcca gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg
    15601 caacctgcat taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca
    15661 gaggaaccat tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat
    15721 tctatgacta tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcgcat
    15781 tctttaattc caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact
    15841 acatcctcca ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct
    15901 ttaatccttt ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc at
//
LOCUS       DADLZM010000096        15881 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLZM010000096 DADLZM010000000
VERSION     DADLZM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.097"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..614)
                     /locus_tag="JNY73_002805"
     CDS             complement(<1..614)
                     /locus_tag="JNY73_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY8048754.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
     gene            complement(611..1873)
                     /locus_tag="JNY73_002806"
                     /pseudo
     CDS             complement(611..1873)
                     /locus_tag="JNY73_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1870..2778)
                     /locus_tag="JNY73_002807"
     CDS             complement(1870..2778)
                     /locus_tag="JNY73_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY8048755.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2944..3741
                     /locus_tag="JNY73_002808"
     CDS             2944..3741
                     /locus_tag="JNY73_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8048756.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="JNY73_002809"
                     /pseudo
     CDS             complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="JNY73_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="JNY73_002810"
     CDS             complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="JNY73_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY8048757.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7187..7366
                     /locus_tag="JNY73_002811"
     CDS             7187..7366
                     /locus_tag="JNY73_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048758.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMFMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7398..7751
                     /locus_tag="JNY73_002812"
     CDS             7398..7751
                     /locus_tag="JNY73_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY8048759.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="JNY73_002813"
     CDS             complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="JNY73_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY8048760.1"
                     /translation="MSYTPMSGLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="JNY73_002814"
     CDS             complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="JNY73_002814"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY8048761.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="JNY73_002815"
     CDS             complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="JNY73_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY8048762.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="JNY73_002816"
     CDS             complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="JNY73_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY8048763.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVSFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="JNY73_002817"
     CDS             complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="JNY73_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY8048764.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="JNY73_002818"
     CDS             complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="JNY73_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY8048765.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13769..14230
                     /gene="hycA"
                     /locus_tag="JNY73_002819"
     CDS             13769..14230
                     /gene="hycA"
                     /locus_tag="JNY73_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY8048766.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14355..14966
                     /locus_tag="JNY73_002820"
     CDS             14355..14966
                     /locus_tag="JNY73_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8048767.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPLLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14963..>15881
                     /locus_tag="JNY73_002821"
     CDS             14963..>15881
                     /locus_tag="JNY73_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY8048768.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGAMSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGII"
BASE COUNT         3622 a         4255 c         4204 g         3800 t
ORIGIN      
        1 cctgcggtca gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc
       61 tccagctctt ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca
      121 tgatttgcca gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg
      181 cgtttatcgc ccggtcggta atagggcacc agcgggacat ttcccatccg catcaccacg
      241 tatggtgtga acggacgaat aacgttgctg ctgtccagcc cttgcaggca ggaaagcgcc
      301 gtcgaccatg tgctgtgcaa atgcaccacc gctttacagc gcggattgtt gcgatacagc
      361 gccagatgaa agagcacctc tttcgagggt ttgtcaccac ttaaccattc gccatccgcg
      421 gcgactttgg aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc
      481 agtaaattcc cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatagccg
      541 cgctgaaaga atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt
      601 gcgaaatcgc tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga
      661 agttgccaga tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc
      721 cgggggaaat ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc
      781 cggaggtctc accacctgcg acaataaagc gtgtcatgcc ttccgctgct aaccgcgccg
      841 ctagttgaga aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt
      901 gaattgctgc caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt
      961 cctggcccag aacccactct gccagttcgt gtgcataagc ggccagagtt tcggttgaga
     1021 ggcagcgtgc cacatcaact tcacgggctg gtgcaatttg acggtaatgt gccacctagc
     1081 ggttggtcat ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc
     1141 ctgcttcgcg agcctggtta ccgttttctt gtgcccactg ccgggccagg ccaatcgcca
     1201 gaccagaacc gcccgttacc agtggggcat cgcgcaaggc ttctccctga atttccagat
     1261 ggtgttcggt cagcgcatca agcaccgcgt agcggtagtc ctcttgctgt aagcgagcca
     1321 gctcttgacg aacggcatcc acaccttgtt cgaaaacatg tgccgaaacg acgccgcagc
     1381 gccctgtgga ttgcgcttca accagacggg gaagatagct gttggtcatg ggatttaccg
     1441 ggtgatggcg catcccggat tcggccagca gttgattcat tacgaaaaaa tacccctgat
     1501 aaaccgtacg tccgttgacc ggcagggccg gagagaagac cgtaaacggc gtgtcgagag
     1561 catccattaa tgcatcggta accgggccaa tattaccttt cgccgtactg tcgaaagtag
     1621 agctgtattt gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca
     1681 gcgattgctg tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg
     1741 cgtcgattgc ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac
     1801 cgttttccac cagaaaactg gcgatatccg tcgcgccggt aaaatcatcg gcgataacgc
     1861 caatcttgat catgatttcg ctcccggtag agtgatgcca gagaaaatct tgataactgc
     1921 gctatcgtct tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt
     1981 tgaggccaat ggcagcggga agtgcagggc tttggctgta tcggcaacca gaccaagatc
     2041 cttaacaaaa atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat
     2101 ccggttttcg aacatccagg aatttccggc ggcattggtc acgacgtcat acatcacatc
     2161 cagcgggatc cccgcacggg ctgcaagtgc catcgcttcg gctccggcag caatatgtac
     2221 gcccgctaac aactggtgaa taatttttac ggtcgaacct agtcccggtt ctgcacctat
     2281 gcgataaact tttccggcaa cggcttccag cacgggtgcc agtcgttcaa aggcaatatc
     2341 gctaccggag gccatgacag tcatttcacc gttagcggct tttactgcac caccagaaac
     2401 tggcgcatcc agcatttcca gatcgaatcc agccagagcg gtagcaattt cttgcgcatc
     2461 agcactagcg atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac
     2521 gcctgtttca ccaaacagca cctgtttaac ctgggccgca ttgaccacca gcaccagcag
     2581 agcgtccagt ttttcggcaa acgtcgcggc gttatcagaa accccgcaag cacctgcctc
     2641 tttcaacgta gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa aacctgcgcg
     2701 gacacatgac agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa
     2761 ctcagatccc gttttcatct gctctccttg ttaatttaag tgatattttg tttgacattg
     2821 tgaatataag cgctgggaga taacgacatg gtgagctgat tcacataaat taacattttg
     2881 tgttatttta tgtgaactaa gcgttagttg cgccgcgcac gtttcgcagg caaatagcgt
     2941 agaatgtcag caggacaaag gaaggagcaa aagttgatac ccgtagagcg tcgccaaatc
     3001 atccttgaga tggtagctga aaaaggcatt gtcagtattg ctgaactaac ggacagaatt
     3061 aatgtgtcac atatgaccat tcgtcgggat ttacaaaaac tggagcagca gggagccgtt
     3121 gtgctggtgt ccggaggcgt ccagtctccg ggacgcgtgg cgcatgaacc ttctcatcag
     3181 gtaaaaactg cgctggcaat gacgcaaaaa gcggctattg gcaagctggc ggcaagtctt
     3241 gttcagccgg gaagttgtat ctatctggat gcgggaacga ccacgttagc gatagcacag
     3301 catctgattc acatggagtc actgactgtg gtcacaaacg atttcgttat tgcgaactac
     3361 ttgctcgaca acagtaattg cacaattatt cacactggcg gtgcagtgtg tcgggaaaac
     3421 cgttcctgtg tcggggaagc cgctgcgacc atgctgcgca gcctgatgat tgatcaggct
     3481 tttatttctg catcgtcatg gagtgtgcgg gggatttcta cgccagcgga agataaagtc
     3541 acggtgaaac gggcgattgc cagtgctagc cgccagcgag ttttggtctg tgatgcgacg
     3601 aaatatggtc aggtggcgac atggctggcg ttaccattaa gcgagtttga tcagattatt
     3661 acagacgacg gtttgccgga gagtgccagt cgcgcgctgg cgaagcagga tctctctttg
     3721 ctggtagcga aaaatgaata atggcctgca ataacatctg gttactcatg cttcacagaa
     3781 gaagcatgag actaccttat tttataaaat gacagccgcc cacttttcgg cgatccggta
     3841 tcaatataaa tctggttagc gaacgtctga atgttatcaa acatcatatg tccaaatata
     3901 aaataatcag cgccgtttat ttgttgtaac tcgccattaa gcgatttctg cacacgataa
     3961 acaggccaga gtaattcgct ctccgctatt tctttgccaa agagatattc atcacccgga
     4021 taatctgcat gtgcgatgac atattttatt atgtcgttag tgatttcaat aatatgtgga
     4081 aggtgatgga atttcagcaa cagatctatt gcctcttgtt gcaatcattt aaatcgaaaa
     4141 actagtcacc accgctggca agccacatat tgccatcgcc ggtcgcgaac gcatccagcg
     4201 ccattgcttc gtggttgcct ttaacggaga taaaccaggg ctggtttagc aggcgcagga
     4261 cgtcaagact ctccggccca cgatcaatat tatcgccgac agaaataagt aagtcggttt
     4321 cggggaaaaa agagagttga tgtaagcggg attgtaataa ctgatattca ccatgaatat
     4381 caccaacgac ccatatatgg cgatagtgat gggcattgat tttttgatag cgtgtagatg
     4441 gcatggtttt accctgtaaa ataagctttc ctattataca gggtattttt atttgattcg
     4501 tcagttgtcg ttaatattcc cgatagcaaa agactatcgg gaattgttat tacaccagac
     4561 tcttcaagcg ataaatccac tccagcgcct gacgcggagt cagtgaatcc gggtcgagat
     4621 tttccagtgc ttcgaccgca ggcgaagttt cttctggcac tgacagcaaa gacatctgcg
     4681 taccatccac ttgcgtcgcg gcggcgttcg gcgaaatgct ttccagctca cgcagttttt
     4741 gccgtgcgcg cttaataacc tctttcggta cgcccgccag agcagcaacc gccagaccgt
     4801 agcttttgct cgccgcgccg tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct
     4861 ccagcgcatc gagatgtacg ttggcgacgc cttccatttt ctccggtagc tgggtcagct
     4921 cgaaatagtg ggtggcaaac agcgtcaatg ccttaatctt attcgccaga ttttctgcgc
     4981 acgcccacgc cagcgacaga ccatcgtagg tggacgttcc acgcccgatc tcatccatta
     5041 acaccagact gtattcggtg gcgttatgta aaatattggc ggtttcagtc atctccacca
     5101 taaaggttga gcgcccggaa gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat
     5161 cgataggtcc aatctcgact ttttgtgccg gtacatagct gccgatgtag gccatcagcg
     5221 caatcagtgc ggtctggcgc atataggtac ttttaccgcc catgttcggg ccggtaataa
     5281 tcaacatacg gcgctgcggc gacagattca gcgggttagc gataaatggc tcattcagta
     5341 cttgttcaac taccggatgg cgaccttcgg taatgcgaat gcccggttta tcaatgaagg
     5401 tcgggcaggt gtagttcagg gtataggccc gttccgccag gttaaccagc acgtcgagtt
     5461 ccgccagcgc gctcgcgctc tgttgcaacg cttccagatg cggcaacagc aggtcgaaca
     5521 gctcttcata aagctgtttt tccagtgcca gtgctttgcc ttttgaggtg agaactttat
     5581 cttcgtactc ttttagctct ggaatgatgt agcgctcggc gtttttcagc gtctggcgac
     5641 gcatgtagtt gatgggtgcc agatggcttt gcccacggct gatttgaatg tagtagccgt
     5701 gcaccgcatt aaagccaact tttagcgtgt ccaggccggt acgttcacgc tcgcggactt
     5761 ccagacgctc cagataatcg gtcgcgccgt cagccagcgc gcgccactca tccagctctt
     5821 cgttatagcc cgatgcgata acaccaccgt cgcgtaccag caccggcggt gtgtcgatga
     5881 ttgctcgctc cagcagatcg cgcagctcgg caaactctcc catcttctca cgtagcgcct
     5941 gtaccggtgc actatcgaca gtttctaact gcgcacgcag ctccggcagt tgctggaaag
     6001 cgtggcgcat acgggccaga tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa
     6061 tacgttccag gtcgccgacc tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca
     6121 atgcgccaat agtttgctgg cgctcaagca acacgcgagt atcgcgcact ggcatatgca
     6181 gccagcgttt cagcatacga ctacccatcg gcgtgacggt gcagtcgagc acagaagcca
     6241 gcgtattttc cgcaccgccc gccaggttct gagtaatttc caggttacga cgtgtcgcgg
     6301 catccataat gatgctgtcc tgctcacgtt ccatggtgat agaacgaata tgcggcaggg
     6361 tcgtgcgttg ggtatctttc gcatactgca acagacaacc ggcagcacaa agtccgcgcg
     6421 gcgcgttctc gacgccaaaa ccgaccagat cgcgggtgcc aaattgcaga ttcaactgct
     6481 ggcgcgcggt gtcgatttca aactcccaca gcgggcgacg gcgcaggccg cgacggcctt
     6541 caatcagcga catctcggcg aaatcttctg catacagcag ttccgccgga ttagtgcgtt
     6601 gcagctctgc cgccatcgtt tcgcggtcgg ccggttcgct caggcgaaaa cgcccggagc
     6661 tgatatccag cgtcgcgtag ccgaaacctt tgctgtcctg ccagatagcc gccagcaggt
     6721 tgtcctgacg ctcctgtaac agggcttcat cgctgatggt gcctggcgta acgatacgca
     6781 caactttgcg ctcaaccgga cctttgctgg tcgccggatc gccaatttgt tcgcagatgg
     6841 caacggactc tccctgattc accagtttgg cgagatagtt ttccaccgca tggtagggaa
     6901 tccccgccat cgggatcggc tctcccgccg aagcaccgcg tttggtcagt gaaatatcca
     6961 gcagttgcga cgcgcgtttt gcgtcgtcat aaaacagttc ataaaaatca cccatccggt
     7021 aaaacagcag gatctcggga tgctgggctt tcagcttgag atactgctgc atcatgggcg
     7081 tatgggcgtc gaaattttct attgcactca tggggttatg tccggttccc tgatattaaa
     7141 tggggtgtga tggtttttat tttcgttgta atcacataag gcgcacatga taccggagtt
     7201 aatcatttca ggggaagaag cgaacggcga gttcttgatg ctggttccgg gaaagatgct
     7261 gttttgcagc ttttgcgtga tgtttatgaa gcaagtacgc aaaattgtat cgattggcga
     7321 acccttcttc tttagagtca tagtgccctt taagattaag atgtaatata aatgcatctt
     7381 aatgaggtgg aggcagcatg tccggcaagc gtatctctcg tgaaaaactg acgattaaaa
     7441 aaatgatcga tctttatcaa gcgaaatgcc cgcaggcgtc agcggagccg gagcattacg
     7501 aggcgttgtt tgtttacgcg caaaagcggc tggataaatg tgtgttcggc gaagagaaac
     7561 cagcctgtaa gcagtgtccg gtgcactgtt accagcctgc aaagcgtgaa gagatgaaac
     7621 agattatgcg ttgggccggg ccgagaatgc tgtggcgtca tccgatctta accgtgcgtc
     7681 atctcatcga tgacaagcgt ccggtgccag aactgccgga aaaatatcgt ccgaaaaaac
     7741 ctcgtgaata gtatgccgga caggcagatc tgtccggcaa tttgcagtta aatcaatgcc
     7801 gatttatcaa ttcccagccg cttcatccgt gacagcaggg tcgtgcgttt cagccccaga
     7861 cgttgcgcag cgcctttagg cccggcaacc acgctgttgg tttctttcag cacgcgcaca
     7921 atcaactgat attcatcttc gccctcctgg gcgacaaccg ttgcggcagg cggcgtttca
     7981 ggctccggta aagcaatatc tggcaatgac agctgcagca cgttgccgcg tgttagcaat
     8041 actgcgcgct caatgacgtt ttccagttcg cgtacgttac ccggccactc catgttgctc
     8101 aaggtgcgca gcgtctcggc aggaatgctg tcgatattgc gccccagacg acgggcaatt
     8161 ttgaaggtaa aggctttcgc cagcagcgga atatcttccg gacgctcgcg tagtggcggc
     8221 aggtgaatcg ggaatacgtt caggcggtaa tagagatcgc tacggaactc tcggtcggcg
     8281 accatttttt tcagatcgcg gttagtcgcg gcaattaaac gtacgtccgt ctgaatgatt
     8341 ttgttgctgc cgagacgttc aaactcctgt tcctgcaata cacgcagcaa cttcggctgt
     8401 aactccagtg gcatatcgcc cacttcgtcg aggaacagag agcttttatc cgccagttca
     8461 aaacgaccaa tgcgctgggc gctggcaccg gtaaaagccc cacgctcatg accaaacaga
     8521 tcgctttcca gcaatccggc aggcatcgcc gcgcagttca ttttgaccat gcggcgatta
     8581 ttacgcccac tgagattatg gatcgcacgg gcaatcagct ctttacccgt gccagtttca
     8641 ccgaggatca gcacggtact gtcactttgc gccaccattt caacttgttt aagcacgctg
     8701 tacatggctt cgctgcggcc aataatctcg ccaaattcac tatcaacatt gttgagctgc
     8761 tcggtcaggg cgaggttttc atcaaccagc cgttctttca gacgatggat ttcctgatag
     8821 gcgagggcgt tatcgacagc gattgccaca cgttcggcaa tctggcgcag caaattcaga
     8881 ttggtagtgg taaacacttt ctcttcgcat tgcgccagtt tcagcacgcc cagcatggtg
     8941 tcgccagaca tcagcggtaa caggcacaag gtttgaatct ggttgcccca ggtgtcgaac
     9001 aacatgcgtt catagggggc taaatcgtcc cgctcgtaga gattgatcag cagcatctct
     9061 ttacttttga acacgcgttc ggtgagggtt ccggcttcat cgacttcgct ctgttcgtgg
     9121 gcgggatgtt gtttatcaag atagtgagtg gagtagatgt tgagtttgtt tttacggtgg
     9181 ctgcgtaaga caatgctgat atcgtcaatg tcgaaatagt aatggatttc tttggcgact
     9241 tcgctgacca gttcgtccat atccaggcgg gaaagcaccg cgttggtaat ggcaaccagg
     9301 atgcggaagt tatcgcgctc ccggcataac aactcatagt cgacattgtt gacgacgcgg
     9361 ctctggattt gttcggtgac gacagaaacg atctgcgtaa atgtttgcag acgattgaac
     9421 tctttttcgc tccagggacg atcgtcataa cgaataaatt cacagccacc aaaaatatgc
     9481 cctcccgccg ccagtggcat caggcaatag tgaccaaatt tggggtatag cccacctgcg
     9541 gccagctgcg gccaggtttc acaaaattct tcgtaactgc aatgcagcgt atcagggcgc
     9601 gacaaaatgc tgcgtaccgg accgtgtgcc agaacagttt cgtcttcata tttaatgggg
     9661 gtgtcttttt cacgcgacgc gtaataagac gcacgttgag tctgcgcttg ccacaacaca
     9721 atagccgcgt tgtcggcgag cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc
     9781 gaggccagat cgggctgctg caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg
     9841 agaccactca tcggtgtata tgacattttg ttcttccagg aaagcaccgc aaagcggtgc
     9901 aaaaggtcat tgactaacta taggagattt agaattttat tagcatatac gcggaagcgg
     9961 ttcggcgtgt ggtaaatcga gggttcgttt cacgccatac agaccggcaa gacgaacacc
    10021 tttacgttcc accacttcac caatcagcgc cgcgtctttc cccagtggat gggaatgtaa
    10081 cgctgccagc acttgctctg ccgcgttgcg ttcaacggcg atcaccagtt taccttcgtt
    10141 ggcaaagttc agggcgtcca gtcccagcaa ttcgcaaacg ccgcgcacgg caggtttaac
    10201 cggcagcgct gattcagaaa tttcaatacc acaaccgcag gctgccgcga actcatgaac
    10261 caccgcgttt acaccaccac gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg
    10321 cagcgtctga ataagcggcg tcagcaccgc gcagtcgctg accagttcgc catccagccc
    10381 cagctgctca cgcaggttaa ggatagtcgc cccgtggtcg ccgagtgtac cgctaaccag
    10441 caatacatcg cctgcggtta gcgtctgtgc gccccagtga atattcgccg gaattgcgcc
    10501 catgccagcg gtgttgataa acagtttatc tgccgcgccg cgctgcacca ctttagtatc
    10561 gccagtaacg atggcaatgc ctgccgtgcg ggcggtttct gccatgctgg tcactgcggc
    10621 tttcagtgtc tccatcggca atccttcttc gaggataaag ccacaggaga gatagcgcgg
    10681 aatagcgcca ctgaccgcaa cgtcattcgc ggtgccgcaa atcgccagct tgccgatatt
    10741 accgccagga aagaacagcg ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc
    10801 gccttccgct accagctgcg ccagatcaag acgtgcctga tcttcctgct ctgccagcca
    10861 cgggttggca aaggcttcca taaacaggct gttgattaat tgctgcatcg cctggccgcc
    10921 gctgccgtgg gcgagttgga tattattcac gcttcactct cctgctgacg atactgatac
    10981 cacgcggcgc acgctccttc ggaggagacc atcagtgcac caaatgcggt ttgaggatta
    11041 caggtgttac caaacagcgg gcattgatgc ggcttacatt tgcccgttaa tacctcacca
    11101 caacgcgcgc gcgggtcatc gcagacctgc tgcggtgccg ggcggaaatg tgcttcggca
    11161 tcgaatcgtt gataatccgg cgtcaggtgc acgccagaag attcaatcac gcctaagccg
    11221 cgccattcgc tgtcgccgtt gacacagaac acatcggcaa tcgcctgttg cgccagcagg
    11281 ttaccggcat ccggtaccac ccgacgatac tgattctcta ccttgctgtg ggccgctatt
    11341 ttctgctcca ccagcatgac cacgccttgc agtagatcaa ggggttcgaa accagcgacc
    11401 accagcggac gatgaaaatc gctggcgata aaattatagg catcagtgcc gataaccata
    11461 ctaacgtggc ccggcgcgag gaacgcgtcg ataccgttat ccggctgttc cagcaaactg
    11521 cgcagcgtcg ggataagcgt aatgtgctgg cagaagaagt aaaaattctg cacatcacgc
    11581 gctttcgcct gttgcagagt gatggcggtg gtcggcatgg tggtttcaaa acctaagccg
    11641 aagaacacca ctttgcgggt tggattctcc tgcgccagtt tcaacgcatc catcggcgag
    11701 taaacgatgc gcacatcggc accgcgtgct tttgcctgca acagcgatcc ctgtttccct
    11761 ggtacgcgca tggcgtcgcc aaaggtacag aagatgactt ccggatggct ggcaatctcc
    11821 acgcaggtgt cgattctacc catcggcagt acgcacaccg ggcaacccgg accgtggata
    11881 aactcaacgt tttcaggcag taactggtcg aggccgaatt taaaaatggc gtgggtatga
    11941 ccgccacaca cttccataat ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca
    12001 cgttcgcgca gatgctcaat taactgcatc acctgttccg gcgcgcgata ttcatcaaca
    12061 aaacgcatta tttttcctcg ccatacaaca gcgcaccgac atccggctca acgtcaaaca
    12121 tgttttgtaa ggcgtcgaga gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa
    12181 agctaacgtg aaccagtacc cactggccca cgcgcggctg accgttttca tcgcagctgc
    12241 cgactaacgt taaatcgaca tcgcgctgaa tgccgcagac gtcgactttc gcctggttgc
    12301 cgtcaatggt gcggatctgg ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt
    12361 cagccactgg tccatccctt cgccgctggt ggcggaaata aggatgattt caatttctgg
    12421 attgacttcg cgggcgcagg cgatgcactt ctcaacgtca aagttgagat acggcaacag
    12481 gtcaactttg ttgagcagca tcagcgaggc ggcggcaaac atatgcggat atttcagcgg
    12541 tttgtcttca ccttcggtaa cggaaagcac cgctactttg tgtttttcac cgagatcgaa
    12601 gctggccggg catacgaggt tgccaacgtt ttcgataaac agaataccgt tatcgtccag
    12661 tggcagacgc ggtgcggcgt cggcaatcat ctgtgcgtca agatggcagc ctttaccggt
    12721 gttcacctga atcgctggtg taccggtagc gcgaatgcgt gcggcatcgt tcacggtttg
    12781 ctggtcgcct tcaataaccg cgcacggaac gctgtctttc aggcgcatta aggtttccgt
    12841 cagcagggtg gttttaccgg aaccagggct ggaaaccagg ttgagcacca gttgcttgcg
    12901 ggcagcaaag cgcgcgcggt tgcgttcagc cagacggttg tttttgtcca gcacgtcaat
    12961 ttcgacttcc agcatccgac gctggctcat gcccggtgtg tgagtgcccg cttcgccatg
    13021 accgtaatgc aggtcgcctt cttcagtctg gctgggggta aattcaggcg ctttaatgcc
    13081 ggtgattttc atctgcgggc gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg
    13141 agggttatgt tcatcaccct cgatatacag gttgccttca ccgcaaccgc atgttgtaca
    13201 catcgctcac tcctggtcta tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat
    13261 ctgtagcatg tcaccatgac actgtggaca gcggcggacg cgctgggtca gtagcgtcac
    13321 atactgttgg catgtttcac accagcattc ggcctcttgt tcttcgaggt gcagtttaca
    13381 accttccgcc acgctgccgc ggcaaaccag atcaaaacaa aaggcaagag agctggtttc
    13441 gacacaagaa aatgcgccaa ttttgagcca gaccccagtt acgcgttttg cgccgtgttt
    13501 tgcggcctgc tgttcgatca attccagtgc ccgttggcag agggttattt cgtgcatatc
    13561 gcctcccatt aactattgcc agctacaagc aataattgcg ccagtgttga ttatccctgc
    13621 ggtgaataat gtcgatgatg tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta
    13681 gcttaaaaat ctctttaata acaataaatt aaaagttggt acaaaaaatg cttaaagctg
    13741 gcatctctgt taaacgagta acctgacaat gactatttgg gaaataagcg agaaagccga
    13801 ttacatcgca cagcggcatc gtcgcctaca ggaccagtgg cacatctact gcaattcgct
    13861 ggttcagggg atcacgttat cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga
    13921 caaagaactc tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg
    13981 ctttaacagc cacaccatcg agtattacgt cgaaacaaaa gatggcgaag acaaacagcg
    14041 gattgcgcag gcgcaactga gcattgacgg catgattgat ggcaaggtca acatccgcga
    14101 tcgcgaacag gttctggaac actatctcga aaaaatcgct ggcgtttacg acagcttata
    14161 caccgccatt gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc
    14221 ggcagcatga gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg
    14281 tgtcgatgag tgtcgaaaat gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac
    14341 ctgaggaatg cctggtgaat cgttttgtaa ttgccgactc cacgctctgt atcggctgcc
    14401 acacctgtga ggccgcctgt tcagagacgc atcgccagca cggcctgcaa tcaatgccgc
    14461 gcctgagagt gatgctcaat gaaaaagaat ctgcgccgct gctctgtcac cactgtgaag
    14521 atgcaccctg cgcggtggtc tgcccggtta acgccatcac ccgcgttgat ggggccgtgc
    14581 agttgaatga aagtctgtgc gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg
    14641 caattgaatt ttccggcagc cgtccgctgg atattccggc aaacgccaat accccgaaag
    14701 cgccaccggc accgcctgct ccggcgcgtg tcagcacatt gcttgactgg gtgccaggta
    14761 ttcgcgcgat cgccgtcaaa tgtgaccttt gtagctttga tgaacaaggt ccggcctgtg
    14821 tgcggatgtg cccgactaaa gccctgcatc tggtggataa caccgatatc gcccgcgtca
    14881 gcaaacgtaa gcgtgagctg acttttaaca cggactttgg cgatctcacc ttgtttcagc
    14941 aggctcaaag tggagaggct aaatgagcgc aatttccctg atcaatagcg gcgtggcatg
    15001 gtttgtcgcc gccgctgttc tggcatttct cttttctttt caaaaagcgc taagtggctg
    15061 gatagccgga attggcggcg cggttggtag tctgtatacg gcagccgcgg gcttcactgt
    15121 actgactggc tcggttggcg tgagcggtgc gatgtcgctg gtaagctacg atgtgcaaat
    15181 ctccccgctt aacgcgattt ggctgattac cctcggcctt tgcggtctgt ttgtcagcct
    15241 ctataacatt gactggcatc gccacgcgca ggtgaagtgc aacggcttgc agatcaatat
    15301 gttgatggct gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat
    15361 ggccgaagtc atggccttgt gtgcggtgtt cctcaccagc aacagcaaag agggcaaact
    15421 gtggtttgcg ctggggcgtc ttggcactct gctgctggtg attgcttgct ggctgctgtg
    15481 gcagcgttat ggcacgctgg atctgcgcct gctggatatg cgtatgcaac agctgccgct
    15541 cggttccgat atctggctgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
    15601 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
    15661 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
    15721 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
    15781 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
    15841 cctgctggct taccacaccc tggaaaatat cggcattatc c
//
LOCUS       DADLZM010000097        15534 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLZM010000097 DADLZM010000000
VERSION     DADLZM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.098"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            208..402
                     /gene="bfd"
                     /locus_tag="JNY73_002822"
     CDS             208..402
                     /gene="bfd"
                     /locus_tag="JNY73_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY8048769.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            475..951
                     /gene="bfr"
                     /locus_tag="JNY73_002823"
     CDS             475..951
                     /gene="bfr"
                     /locus_tag="JNY73_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY8048770.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(948..1415)
                     /locus_tag="JNY73_002824"
     CDS             complement(948..1415)
                     /locus_tag="JNY73_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY8048771.1"
                     /translation="MDTTLPFLILYACLSDLLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1794..2105
                     /gene="rpsJ"
                     /locus_tag="JNY73_002825"
     CDS             1794..2105
                     /gene="rpsJ"
                     /locus_tag="JNY73_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY8048772.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2138..2767
                     /gene="rplC"
                     /locus_tag="JNY73_002826"
     CDS             2138..2767
                     /gene="rplC"
                     /locus_tag="JNY73_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY8048773.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2778..3383
                     /gene="rplD"
                     /locus_tag="JNY73_002827"
     CDS             2778..3383
                     /gene="rplD"
                     /locus_tag="JNY73_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY8048774.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3380..3682
                     /gene="rplW"
                     /locus_tag="JNY73_002828"
     CDS             3380..3682
                     /gene="rplW"
                     /locus_tag="JNY73_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY8048775.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3700..4521
                     /gene="rplB"
                     /locus_tag="JNY73_002829"
     CDS             3700..4521
                     /gene="rplB"
                     /locus_tag="JNY73_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY8048776.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4538..4816
                     /gene="rpsS"
                     /locus_tag="JNY73_002830"
     CDS             4538..4816
                     /gene="rpsS"
                     /locus_tag="JNY73_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY8048777.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4831..5163
                     /gene="rplV"
                     /locus_tag="JNY73_002831"
     CDS             4831..5163
                     /gene="rplV"
                     /locus_tag="JNY73_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY8048778.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5181..5882
                     /gene="rpsC"
                     /locus_tag="JNY73_002832"
     CDS             5181..5882
                     /gene="rpsC"
                     /locus_tag="JNY73_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY8048779.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5895..6305
                     /gene="rplP"
                     /locus_tag="JNY73_002833"
     CDS             5895..6305
                     /gene="rplP"
                     /locus_tag="JNY73_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY8048780.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6305..6496
                     /gene="rpmC"
                     /locus_tag="JNY73_002834"
     CDS             6305..6496
                     /gene="rpmC"
                     /locus_tag="JNY73_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY8048781.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6496..6750
                     /gene="rpsQ"
                     /locus_tag="JNY73_002835"
     CDS             6496..6750
                     /gene="rpsQ"
                     /locus_tag="JNY73_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY8048782.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6915..7286
                     /gene="rplN"
                     /locus_tag="JNY73_002836"
     CDS             6915..7286
                     /gene="rplN"
                     /locus_tag="JNY73_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY8048783.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7297..7611
                     /gene="rplX"
                     /locus_tag="JNY73_002837"
     CDS             7297..7611
                     /gene="rplX"
                     /locus_tag="JNY73_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY8048784.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7626..8165
                     /gene="rplE"
                     /locus_tag="JNY73_002838"
     CDS             7626..8165
                     /gene="rplE"
                     /locus_tag="JNY73_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY8048785.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8180..8485
                     /gene="rpsN"
                     /locus_tag="JNY73_002839"
     CDS             8180..8485
                     /gene="rpsN"
                     /locus_tag="JNY73_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY8048786.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8519..8911
                     /gene="rpsH"
                     /locus_tag="JNY73_002840"
     CDS             8519..8911
                     /gene="rpsH"
                     /locus_tag="JNY73_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY8048787.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8925..9458
                     /gene="rplF"
                     /locus_tag="JNY73_002841"
     CDS             8925..9458
                     /gene="rplF"
                     /locus_tag="JNY73_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY8048788.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9468..9821
                     /gene="rplR"
                     /locus_tag="JNY73_002842"
     CDS             9468..9821
                     /gene="rplR"
                     /locus_tag="JNY73_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY8048789.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9836..10339
                     /gene="rpsE"
                     /locus_tag="JNY73_002843"
     CDS             9836..10339
                     /gene="rpsE"
                     /locus_tag="JNY73_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY8048790.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10343..10522
                     /gene="rpmD"
                     /locus_tag="JNY73_002844"
     CDS             10343..10522
                     /gene="rpmD"
                     /locus_tag="JNY73_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY8048791.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10526..10960
                     /gene="rplO"
                     /locus_tag="JNY73_002845"
     CDS             10526..10960
                     /gene="rplO"
                     /locus_tag="JNY73_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY8048792.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10968..12299
                     /gene="secY"
                     /locus_tag="JNY73_002846"
     CDS             10968..12299
                     /gene="secY"
                     /locus_tag="JNY73_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY8048793.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12331..12447
                     /gene="rpmJ"
                     /locus_tag="JNY73_002847"
     CDS             12331..12447
                     /gene="rpmJ"
                     /locus_tag="JNY73_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY8048794.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12594..12950
                     /gene="rpsM"
                     /locus_tag="JNY73_002848"
     CDS             12594..12950
                     /gene="rpsM"
                     /locus_tag="JNY73_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY8048795.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12967..13356
                     /gene="rpsK"
                     /locus_tag="JNY73_002849"
     CDS             12967..13356
                     /gene="rpsK"
                     /locus_tag="JNY73_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY8048796.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13390..14010
                     /gene="rpsD"
                     /locus_tag="JNY73_002850"
     CDS             13390..14010
                     /gene="rpsD"
                     /locus_tag="JNY73_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY8048797.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14036..15025
                     /gene="rpoA"
                     /locus_tag="JNY73_002851"
     CDS             14036..15025
                     /gene="rpoA"
                     /locus_tag="JNY73_002851"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY8048798.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15066..15449
                     /gene="rplQ"
                     /locus_tag="JNY73_002852"
     CDS             15066..15449
                     /gene="rplQ"
                     /locus_tag="JNY73_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY8048799.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4005 a         3648 c         4132 g         3749 t
ORIGIN      
        1 cgttgttgct aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc
       61 ttcggcgccc tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc
      121 gctgaactat ggcgcaaata aaactgtgct attgtaatca taaccattct catttacact
      181 ttgtgcagaa tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac
      241 aaaaaaattc gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag
      301 tttattcctg tgggaaatca atgcggtaag tgcattcgcg ccgcgcgcga agtgatgcag
      361 gacgagttga tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt
      421 gacatcccgc tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa
      481 ggtgatgtta aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc
      541 aatcagtatt ttctccatgc ccggatgttt aaaaactggg gcctgatgcg tctcaatgac
      601 gtcgaatacc atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt
      661 ttgtttttag aaggaatccc caacttacag gatttaggca aactggggat tggtgaagat
      721 gtcgaggaga tgctgcaatc tgacctgcga ttagaactgg aaggcgcgaa ggatctgcgt
      781 gaggctatcg catatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag
      841 atccttgctg atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt
      901 aaaatcggcc tacaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc
      961 agcaaactat cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc
     1021 gggtttttta atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg
     1081 aatgaggcag caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg
     1141 gcggcgagaa acttcacatc accatagcct aatccttctt tatgtcgcag tatgcgatac
     1201 ccccagtaaa tgacagcgaa tgtgccgtaa ccaacaattg ccccccataa cgcatcggct
     1261 aaaccatcag gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta
     1321 aatctgtcag gtaacaagcc atgctttgca tcccagaaaa aaagtaggtc tgataaacaa
     1381 gcgtacaaaa ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat
     1441 caccatcgaa taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt
     1501 ccggtgaaaa acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg
     1561 cgcgttgcga ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc
     1621 gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc
     1681 taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa
     1741 tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga
     1801 accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg
     1861 cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc
     1921 cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg
     1981 atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa
     2041 ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg
     2101 gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa
     2161 agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga
     2221 agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat
     2281 tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt
     2341 cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga
     2401 agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga
     2461 cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg
     2521 tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca
     2581 gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga
     2641 acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct
     2701 ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa
     2761 ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt
     2821 ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt
     2881 atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt
     2941 ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga
     3001 gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa
     3061 aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc
     3121 aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg
     3181 cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg
     3241 aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta
     3301 tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc
     3361 aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc
     3421 gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt
     3481 tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga
     3541 agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat
     3601 cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga
     3661 cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa
     3721 ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc
     3781 aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc
     3841 cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc
     3901 aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt
     3961 tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct
     4021 aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt
     4081 aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa
     4141 ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt
     4201 gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac
     4261 tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa
     4321 gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtactgc gatgaacccg
     4381 gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg
     4441 tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc
     4501 gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg
     4561 tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa
     4621 gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac
     4681 catcgctgtc cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg
     4741 tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa
     4801 agcgaagaag aaataaggta ggaggaagag atggaaacta ttgctaaaca tcgccatgct
     4861 cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg
     4921 caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtacaggt caagaaagtt
     4981 ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa
     5041 gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa
     5101 ggtcgtgcag atcgtatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc
     5161 tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta
     5221 ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg
     5281 acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc
     5341 gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg
     5401 gtatcgttat cggtaagaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca
     5461 tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa
     5521 aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta
     5581 tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta
     5641 gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac
     5701 cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg
     5761 gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg
     5821 ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat
     5881 aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc
     5941 gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg
     6001 ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg
     6061 cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa
     6121 agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga
     6181 ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag
     6241 cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga
     6301 tgtaatgaaa gcaaaagagc tgcgtgagaa gagcattgaa gagctgaaca ccgagctgct
     6361 gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca
     6421 gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga
     6481 gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac
     6541 aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt
     6601 aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc
     6661 ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg
     6721 gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg
     6781 gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg
     6841 cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt
     6901 agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg
     6961 cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag
     7021 gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg
     7081 atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg
     7141 tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg
     7201 gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta
     7261 tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat
     7321 gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc
     7381 ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg
     7441 gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc
     7501 aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa
     7561 gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta
     7621 gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg
     7681 agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg
     7741 gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag
     7801 caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa
     7861 tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt
     7921 tctttgagcg cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg
     7981 ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc
     8041 cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta
     8101 ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca
     8161 agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt
     8221 agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt
     8281 gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga
     8341 ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct
     8401 gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc
     8461 gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat
     8521 gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa
     8581 caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa
     8641 ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact
     8701 tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc
     8761 aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat
     8821 cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct
     8881 tggtggcgaa attatctgct acgtagccta aatcggagga aaaaatgtct cgtgttgcta
     8941 aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag gttattacga
     9001 tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa gttaaacatg
     9061 cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg gcacaggctg
     9121 gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc ttcactaaga
     9181 agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg attaacctgt
     9241 ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact gctgaatgtc
     9301 cgactcagac tgaaatcgtg ctgaaaggcg ctgataagca ggtgatcggc caggttgcag
     9361 cggatctgcg cgcctaccgt cgtcctgagc cttacaaagg caagggtgtt cgttacgccg
     9421 acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg gataagaaat
     9481 ctgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg ggcgcaactc
     9541 gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca ccgaacggtt
     9601 ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa ctgaagtaca
     9661 ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc gctctggaaa
     9721 aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt cgtgtccagg
     9781 cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt gtaagatggc
     9841 tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa accgcgtatc
     9901 taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag ttggcgatgg
     9961 taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag cgatccagaa
    10021 agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg gcactctaca
    10081 acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg cttccgaagg
    10141 taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg gggttcataa
    10201 cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg caactattga
    10261 tggcctagaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta aatccgttga
    10321 agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa cccgcagtgc
    10381 aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ttgggtctgc gtcgtattgg
    10441 tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg cggtttcctt
    10501 catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg ccgaaggctc
    10561 caaaaaggcg ggtaaacgcc tggggcgtgg tatcggttct ggcctcggta aaaccggtgg
    10621 tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg gtttcgaggg
    10681 tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc gtaaagcagc
    10741 gattacagcc gaagttcgtc tatctgacct ggctaaagta gaaggcggtg ttgtagacct
    10801 gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga aagtgatcct
    10861 ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta aaggcgctcg
    10921 tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg gctaaacaac
    10981 cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc agactgctgt
    11041 ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc cctggtattg
    11101 atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt gagatgttta
    11161 acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg atcatgccgt
    11221 atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg ttggcagaaa
    11281 ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgt tacggtactc
    11341 tggtgctggc aatattccag tcgatcggta ttgctaccgg tctgccgaat atgcctggta
    11401 tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt gtaagtctgg
    11461 tcacaggaac gatgttcctg atgtggttgg gcgaacagat cactgaacga ggtatcggca
    11521 acggtatttc aatcattatc ttcgccggta ttgtcgcggg actcccgcca gccattgccc
    11581 atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg ttggttgcag
    11641 tattagtatt tgcagtgacg ttctttgttg tgtttgttga gcgtggtcaa cgccgcattg
    11701 tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgtcta tgctgcacag agcacacatt
    11761 taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc agtattattc
    11821 tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac tggctgacaa
    11881 caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat gcgtctgcaa
    11941 tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa acagcagata
    12001 acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa acggcgaagt
    12061 atatcgataa agtaatgacc cgcctgacct tggttggtgc gctgtatatt acctttatct
    12121 gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc ggtgggacct
    12181 cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa actctgatga
    12241 tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac ggccgataat
    12301 tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt caagaaatta
    12361 tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg cagtgccgag
    12421 ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca aagttgggtt
    12481 gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat ttgagtatcc
    12541 tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc atagtggccc
    12601 gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta acttcgattt
    12661 atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc gctgaagatg
    12721 ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa gttgccaaat
    12781 ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc ctgatggatc
    12841 ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt cagcgtacca
    12901 agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa tcggggtgat
    12961 tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca agtctctgac
    13021 ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac tgatcgtcag
    13081 ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc tcgcaaatcc
    13141 actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa agaatacggc
    13201 atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc tactattcgt
    13261 gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc gatccctcat
    13321 aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca ggtttgttgg
    13381 agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg tgagggcacc
    13441 gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat tgaacaagct
    13501 cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca gttgcgtgaa
    13561 aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa ctactacaaa
    13621 gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct ggaaggtcgt
    13681 ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc acgtcagctg
    13741 gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc ttatcaggtt
    13801 agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg cgtgaaagcc
    13861 gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga tgctggtaag
    13921 atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga cattaacgaa
    13981 cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg acacaatgca
    14041 gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag tgagttcgac
    14101 gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc tgggtaacgc
    14161 actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg ttgagattga
    14221 tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc tggaaatcct
    14281 gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta ttcttacctt
    14341 gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg gtgatgtcga
    14401 aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt ctattagcat
    14461 gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa ttcattcgga
    14521 agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc ctgtggagcg
    14581 tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg acaagctggt
    14641 catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc gtgcggcaac
    14701 cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc agcctgaagt
    14761 gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg atctggaatt
    14821 gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg gtgatctggt
    14881 acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat ctcttactga
    14941 gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcttgg aaaactggcc
    15001 accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact gagaaggata
    15061 aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc catcgccagg
    15121 ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag acgactctgc
    15181 ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc aagactgata
    15241 gcgttgctaa tcgtcgtctg gcattcgccc gtactcgtga taacgagatc gtggcaaaac
    15301 tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact cgtattctga
    15361 agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg gttgatcgtt
    15421 cagagaaagc agaagctgct gcagagtaat ctgaagcaac gtaaaaaaac ccgccccggc
    15481 gggttttttt atacccgtag tatccccact tatctacaat agctgtactc tttt
//
LOCUS       DADLZM010000098        15249 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLZM010000098 DADLZM010000000
VERSION     DADLZM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.099"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNY73_002853"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNY73_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY8048800.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="JNY73_002854"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="JNY73_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY8048801.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="JNY73_002855"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="JNY73_002855"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY8048802.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="JNY73_002856"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="JNY73_002856"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY8048803.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="JNY73_002857"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="JNY73_002857"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY8048804.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="JNY73_002858"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="JNY73_002858"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY8048805.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="JNY73_002859"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="JNY73_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY8048806.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="JNY73_002860"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="JNY73_002860"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY8048807.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="JNY73_002861"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="JNY73_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY8048808.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /locus_tag="JNY73_002862"
     CDS             7148..7759
                     /locus_tag="JNY73_002862"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY8048809.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNY73_002863"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNY73_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY8048810.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNY73_002864"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNY73_002864"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY8048811.1"
                     /translation="MKITISGTSYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNY73_002865"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNY73_002865"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY8048812.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12358)
                     /locus_tag="JNY73_002866"
     CDS             complement(11999..12358)
                     /locus_tag="JNY73_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048813.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12355..12907)
                     /locus_tag="JNY73_002867"
                     /pseudo
     CDS             complement(12355..12907)
                     /locus_tag="JNY73_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13008..13607)
                     /locus_tag="JNY73_002868"
     CDS             complement(13008..13607)
                     /locus_tag="JNY73_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048814.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13779..14717)
                     /locus_tag="JNY73_002869"
                     /pseudo
     CDS             complement(13779..14717)
                     /locus_tag="JNY73_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14780..15154)
                     /locus_tag="JNY73_002870"
     CDS             complement(14780..15154)
                     /locus_tag="JNY73_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY8048815.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
BASE COUNT         4234 a         3686 c         3497 g         3832 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac
     3661 ccgctctcga cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcagg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgtgctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagct agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg ggccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtga tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcagaata aaaatatttg aattcaaaat gcaacgatcg ccccgaaaaa aaaataatac
    12421 acacaaggat aaaatatcgt cccaacttgt aatttgtaat gttgatatct tttattaaac
    12481 ttgcaattga gatcgcaacc agcaatactg acaatgggta aatgggtgat aaatacctgt
    12541 caccttgcgt aacagaaaac caaaataaga catatgttaa aacaatcgag aaaaataccc
    12601 aaagtatttt attattccta agtgtaaaga cagacagtat agcataaaaa acaacatcac
    12661 tgccaacttt attaattgct tgttgcaaat ctaatgtggc aggacttata ccatgtcgag
    12721 cttgttctgc tgtttgaact tgtagatcaa tttcattcca taggtagtga ccaaatatat
    12781 ttcctccagc aggactaaaa ggatcaccaa agatccagaa actacggaca taccaataaa
    12841 tgccaccaag aattgttgcc aagctaaaaa taagaaatgt ctgtatgttt ctctttttcg
    12901 ctatcaatat aacaaatatg atggctccat atatggcacc aaatagtttt acgccacaag
    12961 caattccaat aaaaaaacca cataaaaaaa ttaattcatt ttgtgtttta ttgttttctg
    13021 agcgaatata tataagaata ctggctgcag caaaacaaag atagcgttaa atcaatatat
    13081 gcataaccaa gatgatcttt aagtggattg atagtaaata gtaggattgt agcaataatg
    13141 ctaggataat aagttttcgt aatttcccat gaacagccaa caagtccaca ggctattaca
    13201 aaaaaacaga tatttgctaa aaactgagct aaccttacat ctcctaactg taaacctaaa
    13261 gccattaaaa gatctgcatt ttgcgggaac agaggaaaac gcaaatattg ttcaacaaca
    13321 attgcatggt gctcaactaa tgaacgagcc aaaggtaagt gatacatagt atcatcccaa
    13381 tcaccgggag ctcgcaggga cgataacaat gtaaccacaa aaatcaaaac aagaaataaa
    13441 ataacaaaca gcgtactgaa ctcaatatta tttattgcca gccatctaaa ttgctgacgt
    13501 ttaataatgc ttaaaaccca acacaccgta aaaattaaaa agatagcaat cttattaaat
    13561 tgcccgacca tgcccagaaa gtaaattatc aaaatattta aagacatccc tatgcatagg
    13621 caataaaaaa ataaagaaaa tttgttaagt cctgccaccc cctccttaaa aaaagatgac
    13681 aatacaatat taccaatgac aaaaaatgtc attccaaaag caaaacaata cacaacatac
    13741 aaatacaacg gcaattcaat catagtaaaa tactctattt acattatcag tttatagttt
    13801 ttttcagaaa gataaaataa tgccttcatt tcacgtctag ttgttgtatt tgaatgcaaa
    13861 ataatgccac atacaaatgc cattattgac gataacatga tggaggcagt taaaaaggca
    13921 gtcggaaatc ttctcaccaa acctgtatca atataatcaa caatgatggg caatcctaaa
    13981 attatagaca aagcagcaag taaaacgccg ataatactaa aaaagtatag tggccgttca
    14041 agcgagtaaa gtttaattat tgttttcaga atcttgaacc catcactcca agttgatagt
    14101 ttgctcacac tcccttcaga tcgctcgcca tattttgtat taacctcacc atacttcata
    14161 cgtaattcta aagcatgaat tgtaagctca gtttcgattt caaatcccct tgatagacat
    14221 ggaaaagatt taacatagcg tctggaaaat acacgatacc ccgacaacat atcagtaaaa
    14281 ttccctttaa agatagtctg aacagtacca gtcaacagtt tatttcctca gcgatgccct
    14341 tttctgtatg tattttgatc tccattctct tgacgacatc ctacaaccat atccaattga
    14401 ttttttatga gatggttaat tatttctgga gcagaacttg catcataagt gccatctcca
    14461 tcaaccatta gataaatatc cgcatctaca tctgagaaca tacgtcttac gacattcccc
    14521 ttgcctttca gaggaacaga gtgaactgtt gctccagcat cctctgccac ttgtgctgta
    14581 ctgtcagtcg aattattatc aaatacatat atagaagcag ttggaatatt cgttttgaaa
    14641 tcatttatga cttttgctat tgccttctgc tcattataac aaggaataat tacagcgata
    14701 ttaaaagagt gatacataag cccctcatat aaactattta gctaatgtgc aagtaattgt
    14761 gtttttaaca aatcgtttac taacgaaaaa tgaaatattt tgatgcgcaa tatcctaaaa
    14821 ccaaactaaa aagagaaaag gtgaacaatg taaatattgg agataaagct aagagatcaa
    14881 ataaaacgcc gaataaataa ctcatcgccc ccataaaaaa tataaatata aaatatctag
    14941 ccttggttgc agatacttta aatgagcatc ttgcatttgc aaaaaaagaa aaggttgctg
    15001 cacatataaa accaacaatg ttacttcggc cttgaccaaa agctaaatta ttataacacg
    15061 cataaaatac cccccaatgt aaagcagtat tgacaattcc aattaaaaaa taactaaaaa
    15121 aagaactggt caataacttc cctattttta acatccttta ttttgctcca gaagtgaggt
    15181 tattactaat ttggatattt tctatagaaa atacccataa aactacaacg cctataataa
    15241 aaattccca
//
LOCUS       DADLZM010000099        15082 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLZM010000099 DADLZM010000000
VERSION     DADLZM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.100"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            29..292
                     /locus_tag="JNY73_002871"
     CDS             29..292
                     /locus_tag="JNY73_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY8048816.1"
                     /translation="MSKLESLPTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLV
                     FAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            302..940
                     /locus_tag="JNY73_002872"
     CDS             302..940
                     /locus_tag="JNY73_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY8048817.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            943..2106
                     /locus_tag="JNY73_002873"
     CDS             943..2106
                     /locus_tag="JNY73_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY8048818.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2190..3814
                     /locus_tag="JNY73_002874"
                     /pseudo
     CDS             2190..3814
                     /locus_tag="JNY73_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="JNY73_002875"
     CDS             complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="JNY73_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY8048819.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="JNY73_002876"
     CDS             complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="JNY73_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY8048820.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4866..5615
                     /gene="yjfP"
                     /locus_tag="JNY73_002877"
     CDS             4866..5615
                     /gene="yjfP"
                     /locus_tag="JNY73_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8048821.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="JNY73_002878"
     CDS             complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="JNY73_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY8048822.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="JNY73_002879"
     CDS             complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="JNY73_002879"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY8048823.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCANDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7894..9291
                     /gene="ulaA"
                     /locus_tag="JNY73_002880"
     CDS             7894..9291
                     /gene="ulaA"
                     /locus_tag="JNY73_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY8048824.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9307..9612
                     /gene="ulaB"
                     /locus_tag="JNY73_002881"
     CDS             9307..9612
                     /gene="ulaB"
                     /locus_tag="JNY73_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY8048825.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9622..10086
                     /gene="ulaC"
                     /locus_tag="JNY73_002882"
     CDS             9622..10086
                     /gene="ulaC"
                     /locus_tag="JNY73_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY8048826.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAVVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10100..10750
                     /gene="ulaD"
                     /locus_tag="JNY73_002883"
     CDS             10100..10750
                     /gene="ulaD"
                     /locus_tag="JNY73_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY8048827.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10760..11595
                     /locus_tag="JNY73_002884"
                     /pseudo
     CDS             10760..11595
                     /locus_tag="JNY73_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
     gene            11595..12281
                     /locus_tag="JNY73_002885"
     CDS             11595..12281
                     /locus_tag="JNY73_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY8048828.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTL
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12410..12685)
                     /locus_tag="JNY73_002886"
     CDS             complement(12410..12685)
                     /locus_tag="JNY73_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8048829.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13012..13407
                     /gene="rpsF"
                     /locus_tag="JNY73_002887"
     CDS             13012..13407
                     /gene="rpsF"
                     /locus_tag="JNY73_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY8048830.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13414..13728
                     /gene="priB"
                     /locus_tag="JNY73_002888"
     CDS             13414..13728
                     /gene="priB"
                     /locus_tag="JNY73_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY8048831.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13733..13960
                     /gene="rpsR"
                     /locus_tag="JNY73_002889"
     CDS             13733..13960
                     /gene="rpsR"
                     /locus_tag="JNY73_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY8048832.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14002..14451
                     /gene="rplI"
                     /locus_tag="JNY73_002890"
     CDS             14002..14451
                     /gene="rplI"
                     /locus_tag="JNY73_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY8048833.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14522..15079)
                     /locus_tag="JNY73_002891"
     CDS             complement(14522..15079)
                     /locus_tag="JNY73_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8048834.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3529 a         3671 c         4058 g         3824 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac ctacattgtt
       61 aggttacgtt atcccattat ccagtgcggc aatcaatgcg gtgagtattc cagactattt
      121 cgcctggggc gggattgcac tggtgattca gttactcgtt tttgctggtg tcagacttta
      181 tatgcctgca ttaagcgaaa aaattattaa tcacaatacc gcagcaggaa tgtttatggg
      241 aaccgccgcg ctggctggcg gtatttttaa cgcagcttgt atgacatggt aatggacgat
      301 catggccaga aaacgcaaat caagaaataa cagtaaaatt ggtcacggag cgatcagtcg
      361 cattggtaga ccgaataatc cttttgaacc gcgtcgcaat cgctacgcac aaaaatactt
      421 aacgttggcg ctaatgggcg gtgccgcttt tttcgtattg aaaggttgta gcgatagtag
      481 cgatgtcgat aacgacggcg acggaacgtt ttacgcgaca gtgcaggatt gtattgatga
      541 cggtaataat gccgacattt gcgcgcgtgg ctggaacaac gccaaagcgg cattttatgc
      601 cgatgttcca aagaatatga ctcagcagaa ctgtcagtct aagtacgaaa attgctacta
      661 cgacaatgtt gaacagagtt ggatcccggt cgtttctgga tttttgttaa gccgggttat
      721 tcgtaaagat cgcgatgagc cgttcgtcta taacagcggc ggatcctcat ttgcttcccg
      781 ccccgtctgg cgcaacactt ccggtgatta ctcctggcgc ttcggttctg gcaaaaaaga
      841 gtcttactct tcaggcggct ttaccaccag aaaagcgtcg accgtttcgc gcggcggcta
      901 tggtcgttct tccagcgccc gtgggcattg gggaggctaa tcatgctgag acacaacgtt
      961 cctgtgcggc gggatctgga ccagatcgcc gctaacaacg gtttcgactt tcatatcatc
     1021 gacaatgaaa tctattggga cgagagtcgg gcttaccgtt ttactctgcg ccagattgaa
     1081 gagcagatcg aaaaaccgac tgcggaactg catcagatgt gccttgaggt ggtggatcgc
     1141 gcggttaaag acgaagagat cctgacgcaa ctggcgatcc cgccgttgta ctgggatgtg
     1201 atcgccgaaa gctggcgcgc acgcgatcct tcgctgtatg gccggatgga ttttgcctgg
     1261 tgtggcaatg cgccggtgaa gctgctggag tacaacgccg atacgccgac ctcgttgtat
     1321 gagtcggctt atttccagtg gttgtggctg gaggatgccc ggcgcagcgg cattattccg
     1381 cgtgatgccg atcagtacaa cgccattcag gaacgcctga tttcgcgctt tagtgagctt
     1441 tacagtcggg aaccgtttta tttttgctgc tgtcaggaca ccgatgaaga caggagtacc
     1501 gtgctgtact tgcaggactg cgcccagcag gcagggcagg agtcgcggtt tatctacatt
     1561 gaagatctcg gtttgggcgt cggcggcgta ctgaccgatc ttgatgataa tgtcatccag
     1621 cgtgcattta agctgtatcc gctggagtgg atgatgcgtg acgataacgg tccgctgctg
     1681 cgcaagcgtc gcgagcaatg ggtggagccg ttatggaaaa gtatcttgag taataaaggg
     1741 ctaatgccgc tgctttggcg cttcttccct ggtcatccta accttcttgc atcctggttt
     1801 gagggtgaaa aatcgcagat tgccgctggc gaaagctatg tgcgtaaacc gatctactcg
     1861 cgcgaaggcg gaaacgtcac cattttcgac ggtcagaata acgtcgttga ccacgctgat
     1921 ggtgattacg ccgatgaacc gatgatctac caggcgtttc aacctctgcc gcgatttggc
     1981 gatagctaca cactcatcgg tagctggatt gtcgatgatg aagcgtgcgg aatggggatc
     2041 cgtgaagata acacactgat caccaaagac acctcacgtt tcgttccgca ttacattgct
     2101 ggataagaat gttttagcaa tctctttctg tcatgaatcc atggcagtga ccatactaat
     2161 ggtgactgcc attgatggag ggagacacag tgcactggca aactcacacc atttttaatc
     2221 aacctatacc attaaataac agcaacttat acctgtctga tggcgcgctt tgcgaagcgg
     2281 taacgcgtga aggtgctggc tgggatagcg attttctagc cagtattggt cagcagttag
     2341 gaacggctga atcccttgaa ctggggcggc tggcgaatgt aaacccgcct gaactgttgc
     2401 gctacgatgc ccaggggcgg cgtctggacg atgttcgttt ccatcccgca tggcacctgc
     2461 tgatgcaggc gttatgtacc aatcgggtgc acaatcttgc ctgggaagaa gacgctcgct
     2521 ccggcgcatt tgtggcgcgc gcggcgcgtt ttatgttaca tggcaggttg aggctgggtc
     2581 gttatgtcca ataacgatga cctttgccgc cacgccattg ttgttacaga tgttacccac
     2641 gccgtttcag gactggacca cgccgctgct gagcgatcgc tacgattctc acttattgcc
     2701 aggtgggcaa aaacgcggtt tgttgattgg catgggaatg acggaaaagc agggcggttc
     2761 cgatgtcatg agcaacacca tccgtgcaga gcgtttggaa gatggctctt atcggctggt
     2821 ggggcataaa tggtttttct cggtgccgca aagcgatgcg catctggtgc tggcgcagac
     2881 cgcgggcggt ctgtcctgct tttttgtgcc gcgctttttg cctgacgggc aacgcaacgc
     2941 gattcgcctc gagcggctga aagataagct gggtaatcgc tctaacgcca gttgcgaagt
     3001 ggagtttcag gatgccattg gctggttatt ggggcaggaa ggggaaggaa ttcgtctgat
     3061 cctgaaaatg ggggggatga cgcgttttga ttgcgccctg ggtagccatg ccatgatgcg
     3121 ccgtgcattt tcgctggcga tttatcatgc acatcaacgc catgtttttg gtaatccatt
     3181 gatccaacag ccccttatgc gtcatgtctt aagtcgcatg gcacttcagc ttgaagggca
     3241 aacggcgttg ctgtttcgtc ttgcgcgagc gtgggaccgg cgtgccgatg ccaaagaagc
     3301 cctgtgggcg cgtttattta cgcctgcggc aaaatttgtg atctgcaaac gaggtatgcc
     3361 gtttgtggcc gaagcgatgg aggtgctggg cggcattggt tattgcgagg agagcgagct
     3421 gccgcggctt taccgggaga tgccggtaaa cagtatttgg gaaggttccg gcaatattat
     3481 gtgcctggat gtgttgcgcg ttctcaataa gcaagcgggc gtatacgact tattgtcgga
     3541 agcatttgtg gaagtgaaag ggcaggatcg ctattttgat cgcgcggttc gtcgtttaca
     3601 gcagcagctg cgtaagccag ctgaagaact ggggcgagag attactcatc agctattcct
     3661 gctgggctgc ggtgcgcaaa tgttgaaata tgcttctccg ccaatggcgc aggcgtggtg
     3721 tcaggtgatg ttagatacgc gcggcggcgt acggttgtca gagcagatcc agaatgattt
     3781 attgctgcgg gcgacggggg gagtgtgtgt gtaagcgtat acgactgatg cgacgctggt
     3841 ttcgattaac taaatgaaat atgtgaaaat tgtaggccgg acaaggcgct cgcgccgcat
     3901 ccggcattgt tcatttgctg tagatgacac tcatgcatac agtatcgcct gtacgcgcca
     3961 gttatcaggc tgctggtctt cgtacatggt aataatgcgg taccatgacg caccttgttc
     4021 atcggccttt aacgccacga tacgttccac gtcctgcgga tcccctgaaa tattgctaac
     4081 tgagatctga cctatttcat tcaggcccgt cacgcagtca gcactggcga attctgcaga
     4141 ctgtgccgtg gcgctcgcag gataagtcac taattgcagc gagggtgagg caagtaattg
     4201 tttcatcgtc agctccattt ttcgtttccc cacatgttgc agggcattcc atcgcggtaa
     4261 taaacgccca agcttccagc cggtaattta ttgtgcacag gtaaacgcat agggacgcaa
     4321 agcaatgtaa agctgactat gtaagttgtc tgatttattt acggtacaaa atggcttgtg
     4381 aataccactg tcctgtcacg atggtttcgt ccaccatgac gacaacgtaa taatcagctt
     4441 ttgcagcgac agcttttgct ctgatttctg ctaatgcgtc atccggagaa ccccgaacca
     4501 tcgtgcttac gctacctatt cgctgtaacc cttgcgtctg gtcgcggcga atctcttgcg
     4561 gatggtccgt tactggcggt gctggctgtg gcgtaccttg cagtgcgcta caggcactta
     4621 acatcaacac caataataaa ctggcaaacc ggtaaataac gctattacgt ttcctgctaa
     4681 ccatagtgta gtgccttatt aatttaactt tgggggaatg ttcccgaatt gttacctggt
     4741 acgcgatttt cgaatgaaaa cgtgacgaaa gcgtaaccca catctcaaca ttatgaacta
     4801 ataccgccag cagaaacttg tgctaaggat ttcttcatgt cataccagac aaaaaggaga
     4861 gacagatgat tgaaatagaa tcacgcgagc tggcagatat tcccgttctt catgcttatc
     4921 ctgtcgggca aaaagatacc ccgttaccgt gcgtaatttt ttatcacggc tttacttcat
     4981 ccagcctggt gtatagctat tttgccgttg cgctggcgca ggctggtttg cgggtgatca
     5041 tgccggatgc gcccgatcac ggtagccgtt ttagtggcga cgaagcgcgg cggttaaatc
     5101 aattctggca aatcttgcta caaagtatgc aggaattcac tactttacgt gcggcaatag
     5161 ccgaagaaaa ctggctgctt gatgaccgtc tggcagtcgg tggcgcgtcg atgggcgcga
     5221 tgacggcact gggaattacc gctcgccacc ccacggtgaa atgtaccgcc agcatgatgg
     5281 gatcgggcta ttttacatcg ctcgcccgtt cactgtttcc accgctgata cctgaaacgg
     5341 cagcacagca gaatgaattc aataaaattg tcgcgccgct ggcagagtgg gaagcgacaa
     5401 accacctgga acaacttggg gacagacctc tactgctgtg gcatggcctc gacgatgacg
     5461 ttgtgcctgc cgacgaatca ctacgtttgc agcaggcctt aagcgagacg ggacgggata
     5521 aactgctaac ctgttcatgg cagccaggcg tgcgtcaccg cattacgcct gaggcgttag
     5581 atgctgccgt gacatttttc cgccagcatc tttaaacacg cagaatgctg accccttgcg
     5641 cttccagttg ttgcaggatt tccgggttag catttttgcc ggtgatcagc atatcgattt
     5701 gatcggcacg gctaaaaagc attcccgcgc gttcgccaat cttactgcta tcaaccagta
     5761 ccaccagttt ccctaccacg ctcagcatct tctgctctgc cattgctgtc agcatatcgg
     5821 ttttatacaa cccttctgcg gtcagccctt ttccgctggt aaacatccag tgcccggcat
     5881 agagactgtt ttcgctgccc tgcgggctta aagtgatgga ctgacttttg ttgtactgtc
     5941 cacccataat gatcacgctg tcatgttctt gatcgatcag gtaatttgcc agcggtagat
     6001 aattagtgat gatttgcact ggcttgccac acatttcccg cccaagcaga aacgcggtgg
     6061 agccgcagtt gatgactacg ctttcgcccg gattaaccag ctgcgaggcc gctttagcaa
     6121 tacgtacttt ttcatcgtga ttctgcgcct gatgcagatt catcggcgtc cagcgcgggc
     6181 gctgttgggt aatagcttct gcgccattgc gcaccttttt cagtttgccg ctttcgccaa
     6241 gtttattgat atcgcgtcgc gcagtggcag gcgaaattcc cagacgctca acgactttct
     6301 caacggtcac aaagcccaat tgtgcgagca tttccaggag gatttgatgt ctttgtgctt
     6361 cagtcatgag ctattccgat aaaatttgat ttttttagat gatatttgaa atagccagga
     6421 aatactacgc cggatagcgc gaacttctcc acgctatccg gtataaacga cagattacag
     6481 gaatgacttg aacggcagat ccggttcaat agtgaagcaa tcatcgaaac cgcgcggata
     6541 gtggtactcg aagttgtctt tatccagcgg ccaggtaaat ttgccgccaa cctgccagat
     6601 aaacggcttg aagccatact tcagacgatc ttttttcatc tcccacagca cgcggatctc
     6661 ttgcggatcg gcctggaagt ttgaccagat gtcgtggtgg aacgggataa ccactttcgc
     6721 attcagggct tcacccatac gcagcatatc ggcgctggtc attttgtcgg taataccgcg
     6781 cgggttctca ccatacgatc ctaacgccac atcgatctga tgttcgttac cgtgcttcgc
     6841 ataatagtta gagtagtggg aatcgccact gtgatatagg gaaccgccag gggttttgaa
     6901 caggtagttt actgcgcgat cgtccatgcc atctggcagt acgccagccg ctttttgatc
     6961 ggcaggcagg gtgatcagtg cagtacggtc gaaagcgtca agcgcatgaa tttcaatgtc
     7021 tttcactttt acaacatcgc ccggtttgac cacgatgcaa cgctctttcg gtacgcccca
     7081 gccaatccac aaatcaacgc aggttttcgg tccaatgaat ggtacgtcat tagcacagtt
     7141 ctgcatcacg gcagcagcga cgttaacgtc gatatgatcg ttgtgatcgt gagtcgccag
     7201 taccgcgtcg atctggcgaa tcgcaaacgg atcaagaaca aacggggtgg tgcgtaggtt
     7261 tggctgcagt tttttcacgc cagccatgcg ctgcatctgg tgaccctgtt tcattaacgg
     7321 gttaccgtga ctttgtttgc cagtgccgca ccagaaatca acgcaaacgt tggtgccacc
     7381 ttctgatttc aaccagatcc cggtgcagcc tagccaccac atcgcaaatg tgccaggggc
     7441 gacctgctct tgttcaattt cttcattcag ccagctaccc cactccggga aagtgctcag
     7501 gatccaggat tctcgggtga tacttttcac tttactcatc gccatttacc ttcatgatag
     7561 ttcaattcga atcaatatgt gattggtttt gattaatcct gacactattt tttcaggaag
     7621 gcaatgacca ttttttgact ttttgccagg gaagttgttg ttgattttga gtgtagaaag
     7681 atttaatgga atgtatagat aaattaacta aatgaattta aataaataat tttttcgttg
     7741 tgtgaatccc actctatccg tgtggaatta tttgcgggtc gcgccacatt taatcataaa
     7801 taatcttgtt gtgattactt ttgaaaatta gagtgagtgc acaacattcc gggtgtgcgg
     7861 aatacccggt tacctcttct tcaggagatc gttatggaga tcctctacaa catctttacc
     7921 gtgtttttta accaggtcat gaccaatgcc ccgttgttgc tgggtattgt gacctgtctg
     7981 ggctacatcc tactgcgcaa aagtgtcagc gttattatta aaggcacgat taaaaccata
     8041 attggtttca tgttgttgca ggcagggtcc ggcatcctca ccagcacctt caaaccggtg
     8101 gtggcgaaaa tgtccgaagt ctacggcatt aacggcgcaa tttccgatac ttacgcgtcg
     8161 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     8221 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     8281 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     8341 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8401 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8461 ttctccatcg gtcaccagca acagtttgca tcattaattg cctataaagt tgcgccgttc
     8521 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8581 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8641 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8701 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8761 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8821 gttctggcga ttgactgtgc ggctatttat agcttcgctc cgaacgccgt ggtctggggc
     8881 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8941 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     9001 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     9061 atcgaaatct ttggttgcgt ctgggtggtg aaacttaccg gtatgagtgc ctggatgggg
     9121 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     9181 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     9241 ctgcgcgcag aagaagatgc agaaaaacaa ttggcagaac agtctgctta ataaggagtt
     9301 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9361 atgaagatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9421 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9481 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9541 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9601 gatgtgaagt aaggacgcgc catgaaatta catgattcgc tggcggagaa taaatccatc
     9661 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9721 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9781 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9841 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9901 gaagataacg acccggtgga tatcctcatc accatggcgg tggtcgatgc caacacgcac
     9961 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
    10021 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
    10081 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
    10141 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
    10201 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
    10261 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
    10321 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10381 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10441 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10501 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10561 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10621 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10681 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10741 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10801 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10861 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10921 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10981 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    11041 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    11101 ccagctgcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    11161 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cacaggtgac gctggcgatg
    11221 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    11281 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    11341 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11401 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11461 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11521 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gcatggccaa agcaggcatg
    11581 gtggaggcgg cataatgcaa aagctaaaac agcaggtgtt tgaagccaac atggatctgc
    11641 cgcgctatgg gctggtgacc tttacttggg gcaacgtcag cgctatcgac cgcgaacgcg
    11701 ggctggtggt gatcaagccc agcggcgttg cctacgaaac catgaaagca gacgatatgg
    11761 tggtggtcga tatgagcggc aaggtggtgg aaggggagta tcgcccatct tccgacactg
    11821 cgacgcatct cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc catacccact
    11881 ccactcatgc caccgcatgg gcgcaggcgg ggctggcgat cccggcatta ggcaccacgc
    11941 acgctgacta cttctttggt gatatcccgt gtacgcgtgg attaagcgaa gaagaggtgc
    12001 agggtgagta tgaactgaac actggcaaag tgattatcga aacgctgggc aacgccgagc
    12061 cgctgcatac gctgggaatt gtggtgtatc agcacggacc tttcgcctgg gggaaagatg
    12121 cccacgacgc ggtgcataac gcggtagtga tggaagaagt ggcgaaaatg gcgtggattg
    12181 cgcgcagtat taatccacaa ctcaatcata tcgacagcta cctgatgaat aaacacttca
    12241 tgcgtaagca cggtcctaac gcttattacg ggcagaagta aaacacgcgc tgcggaaatt
    12301 tccttcctcg ggagataact ggtctaattc cgcagccgtt tttcaaaaaa agccccctgc
    12361 gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc tagcgataaa
    12421 tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga attacgtggt
    12481 agtacgttgc gccttgcgca tctgtttctt catttaatgc ctgacgtgct tcgctttcag
    12541 tggcgaaatt atgattgata taaatcacgc ctaagctttg cacatcgtcc atatttctgg
    12601 cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa agcacagcca
    12661 gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt gtgtgctctc
    12721 ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat ggtgaccatt
    12781 tctgatgcag ttgttcaaaa aaacactgtg acggaatgtg atgaacttca aatcagcggt
    12841 ttagaggtta tttgcgaaag gggagattta tttctgctcc gcccttgagt ttagcggggt
    12901 atgtcagtac tataacgcgt catttttcag ccgaccttta acacgttcct tgcctccccg
    12961 ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat
    13021 tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg catgatcgag
    13081 cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga agactggggc
    13141 cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt tctgatgaac
    13201 attgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt caacgatgcc
    13261 gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt
    13321 aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat
    13381 gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt gttgtccggc
    13441 accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca ctgccagttc
    13501 gtgcttgagc atcgttctgt gcaggaggaa gccggctttc accggcaggc atggtgtcaa
    13561 atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat aacggtcggc
    13621 agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg actgagcaaa
    13681 atggttttgc acgccgagca gattgaattg atagattctg gagactagcc atatggcacg
    13741 ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag agatcgacta
    13801 taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg tcccaagccg
    13861 tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca aacgcgctcg
    13921 ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg tccattaata
    13981 cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc aaacctgggt
    14041 agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag
    14101 ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg tcgcgctgaa
    14161 ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga gaaaatcaat
    14221 gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc
    14281 atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt ggctaagagc
    14341 gaagttcgtc tgccgaacgg cgttctgcgt accactggtg aacacgaagt gagcttccag
    14401 gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata attcgttatt
    14461 caacgagacg taaaaacgcc gaccattggt cggcgttttg ctttctattt ttcgtcaggc
    14521 attaatttcg caagtagatc cattcaccat tctcttgtgt ccataagtca ggatggtttg
    14581 ctggcgttag atgcatacga tcactcctta tgtctcggtc ctgcggcact cgctcgaaac
    14641 ctaaacccga gtagattgcg gctgaatttg ataaggaacg tagaccaatt ttcaaatcat
    14701 ggggtaaatc agaatacgcg tttagcaatg cagcaatgat gaatccgagg gcattagaaa
    14761 cgcctgtaac agtgcgaagt tggtcgatat aaagatcgat tgttggtagt tcagatgacc
    14821 agcaattagg catttgggca ggaagggtta caacgtatcc tatcgtttgg ccggtatagt
    14881 ctggagtact ataaccaggt cccaaaacaa cctcgtgtat tgtgtattta ctgttgggtt
    14941 cgaaggattc gacatcttgc aataaatatt tcagttctgc ggttaacctc gcacccgcgt
    15001 aagctttatt gtatttctgg gcgtgaatat tatctaatgt agcttcatgg ttccatccaa
    15061 aagaattatc accattcatt gt
//
LOCUS       DADLZM010000100        14935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLZM010000100 DADLZM010000000
VERSION     DADLZM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.101"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            596..793
                     /locus_tag="JNY73_002892"
     CDS             596..793
                     /locus_tag="JNY73_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048835.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(743..1936)
                     /locus_tag="JNY73_002893"
     CDS             complement(743..1936)
                     /locus_tag="JNY73_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8048836.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2072..3796
                     /gene="iucA"
                     /locus_tag="JNY73_002894"
     CDS             2072..3796
                     /gene="iucA"
                     /locus_tag="JNY73_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY8048837.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3797..4744
                     /gene="iucB"
                     /locus_tag="JNY73_002895"
     CDS             3797..4744
                     /gene="iucB"
                     /locus_tag="JNY73_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY8048838.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4744..6486
                     /gene="iucC"
                     /locus_tag="JNY73_002896"
     CDS             4744..6486
                     /gene="iucC"
                     /locus_tag="JNY73_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY8048839.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6483..7799
                     /gene="iucD"
                     /locus_tag="JNY73_002897"
     CDS             6483..7799
                     /gene="iucD"
                     /locus_tag="JNY73_002897"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY8048840.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7805..10000
                     /gene="iutA"
                     /locus_tag="JNY73_002898"
     CDS             7805..10000
                     /gene="iutA"
                     /locus_tag="JNY73_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY8048841.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10955..14842
                     /gene="sat"
                     /locus_tag="JNY73_002899"
     CDS             10955..14842
                     /gene="sat"
                     /locus_tag="JNY73_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY8048842.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSRTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4025 a         3554 c         3708 g         3648 t
ORIGIN      
        1 gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg ctaataaaat
       61 catggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa gagttttagt
      121 tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag gagttggtcc
      181 caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca tcagtgagtc
      241 aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat taaaaaccag
      301 taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg tcagtgaaaa
      361 agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc gccaatgccc
      421 tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc tgtttgaacg
      481 cgcagcgcag actcccggaa taaaagctga ctggcttgtc agaatgaaat ttaataacag
      541 agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa gaatcatgga
      601 gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc agcaagccag
      661 aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc tgatttactc
      721 cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata aatacagaca
      781 caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt gcgacacctg
      841 ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg tgactggtag
      901 cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt tgataagcgg
      961 gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac aaccatagct
     1021 gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc aggagcaggg
     1081 ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc gcagctactg
     1141 cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc gtagcgaact
     1201 ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca gccagtaata
     1261 ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg cactccggtg
     1321 tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc actggggctg
     1381 ccatcagcag ccccagtggc gccagaggat gaatggccag cattgctgcc gcgcacagcg
     1441 gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg gccatgcgat
     1501 tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt gccgacacgg
     1561 tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct gttgttatgg
     1621 cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg ccatatcctc
     1681 ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt ccccaccacg
     1741 gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat acagacagac
     1801 tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc tgtccgatac
     1861 cgagaagccc ggcactgaac gcaagtggcc agcaggacag tggtttttgg ggcgtatctt
     1921 cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt atttgtaaaa
     1981 ttaatttatc tgacaataac atttattgtt gataatgaga atcattattg acataattgt
     2041 tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa aaccagccac
     2101 agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag actggaaact
     2161 gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat cgctccattt
     2221 cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg cacgtctggt
     2281 cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta ttattgataa
     2341 gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc agcgcgtgct
     2401 ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg ccgctctgcg
     2461 tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac acgctttcca
     2521 ccctgcgcct aagtctcatg aaccgtttaa ccggcgggag gctgaacgct acctgcctga
     2581 tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa tcgccggtga
     2641 aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga atgcgcctca
     2701 gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt ggcaggggga
     2761 atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga ttaaagactt
     2821 aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct actgtgccac
     2881 cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca tccgtactct
     2941 gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa ccgacggctg
     3001 gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg gctgggccgg
     3061 gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc gtgaaaatct
     3121 gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc aggccgcacc
     3181 tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc gcctcggtat
     3241 cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag tgctaaagcc
     3301 gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc aaaatattct
     3361 tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc agggcagcgc
     3421 ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg cggaaaatat
     3481 tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta actccacttt
     3541 tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc tgatggctcg
     3601 tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct gtctcaacta
     3661 cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc tgaacgatca
     3721 taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta acccgctgca
     3781 ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg gactgcgctg
     3841 tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg cggtgttgca
     3901 ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga tatttatcgc
     3961 cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg agccacaggc
     4021 gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct tctggcagtt
     4081 accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga
     4141 gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt accgccgtta
     4201 cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga
     4261 acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc aaagtggctc
     4321 actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg cattcccgct
     4381 gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg cggcggaaga
     4441 ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc tgctggttgg
     4501 tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc tgacacatta
     4561 cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca ccgataacca
     4621 gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt tcgacttccc
     4681 acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg aggtcggtct
     4741 gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa aatgttgtct
     4801 gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca ctactgcatt
     4861 aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg ctggctgtgg
     4921 attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac gctgctgatg
     4981 cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat gcaggatttg
     5041 tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact gagtgccagt
     5101 gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca tcctaaattc
     5161 gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata tgcgccagag
     5221 tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat gatctggcgc
     5281 tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc gcaggagttt
     5341 acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct gccgctgccg
     5401 gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga ttttgccgaa
     5461 ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca gtcgctgcgt
     5521 accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct gaccatctac
     5581 aatacctcat gctaccgggg gatccctggc agatatatcg ctgccggacc actggcttca
     5641 cgctggctgc aacaggtttt tgcgaccgat gccaccctgg tgcaaagcgg cgcagtgatc
     5701 cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct tgcccgggct
     5761 ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg ccgctggctg
     5821 aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga aaacaatcag
     5881 ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg gcttacgcaa
     5941 ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg tgtcgcgctt
     6001 attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca gcgtgttctg
     6061 ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga aatggactct
     6121 ttgcctcagg aggtgcgtga tgtcacatcc cgtctgagtg cggactactt aatccatgat
     6181 ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat ggttcgtctt
     6241 ggcgtacctg aaaggcgatt ttatcaactg ctggcagaag tgttgagtga ttacatgaac
     6301 aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc acaaatcatt
     6361 cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg cagtcgcatg
     6421 ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa
     6481 tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat ctcagcattg
     6541 ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa catcctcatt
     6601 tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc tttctgaaag
     6661 atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat ctggtgaagc
     6721 acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt gaagagtttt
     6781 ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt cataccgttg
     6841 aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt cggggagaat
     6901 attttgccca caatatctgc cttggtacag gaaaacaacc ttatttacca ccctgtgtga
     6961 agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctga
     7021 gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg ttccttaatg
     7081 cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg cgtaataatt
     7141 ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa tatatttcag
     7201 gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag aaaatgacat
     7261 cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac caccgttttg
     7321 aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta acaactctgg
     7381 aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag ggcagagaga
     7441 gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg ccacaaatac
     7501 ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa gtgcgtgatg
     7561 acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc aacgccagca
     7621 tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga tctgcacgta
     7681 ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc gcactgattc
     7741 agtggcgcag cggcagccct gcttctttaa ctcactatac agcaaatatt caggaataac
     7801 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgctgtc
     7861 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     7921 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     7981 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     8041 tgtcagcagc caaagtcgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     8101 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     8161 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     8221 cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa ggtcagccgg aaaccatgat
     8281 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     8341 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     8401 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     8461 cggcctgcag cactctaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8521 atcccggcag cttcaactga taacgcagta ctataaaagt cagggagacg acaattacgg
     8581 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     8641 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8701 tgacagtgat ttcctgggac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     8761 gcggtactac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     8821 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     8881 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     8941 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     9001 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     9061 cattaatgat atttttaccg ttagcggtgg cgtacgctat cagtatactg agaacagggt
     9121 agatgatttc atcgactaca cgcagcaaca gaagattgct gccgggaagg cgatatctgc
     9181 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     9241 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     9301 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     9361 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     9421 tgaactgggc tggcgcttta tcggtgacaa cctgcggact caaatcgcgg catattactc
     9481 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     9541 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     9601 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     9661 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattaact gggcgccgga
     9721 accgtggagt ctgcgtgtac agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     9781 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggcagcttc cggtgggaac
     9841 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     9901 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     9961 gggccgaacc tttggtctga actactcagt gctgttctga cggggatgct tttgcaacaa
    10021 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
    10081 cagccttaac aagatattcc gtctccggaa tgggctgttc accactgaat aacattatgt
    10141 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
    10201 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctgag
    10261 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
    10321 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
    10381 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
    10441 attgttaata cgatggtgca cgatgcatcg tcagtgcccg gcccggagga tgccccgtag
    10501 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
    10561 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga
    10621 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
    10681 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
    10741 ccacattggg caaaaaaaaa caaaaaacga tcaattaacg atcaattaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa
    11701 tcttgaaata ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa
    11761 atgggtatta gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg
    11821 gataacaaaa tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat
    11881 aaatctgaat ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa
    11941 tgcagatacc actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa
    12001 tgggggaaat gttctgttta aggataattt ggattttggt agcggtggta ttatctttga
    12061 cgaaggccat gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga
    12121 tatcggaaaa gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca
    12181 caaaataggc cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg
    12241 tgaaggaaat gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg
    12301 aaatggtaag gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat
    12361 attttttact aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag
    12421 aattgccgcc actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt
    12481 tctggcaatc aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa
    12541 actaacgcac aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg
    12601 tagtgtcaac acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca
    12661 tgcaacagag catgcaatat tcagaagcag aacgagtcat tgctccctgg tatttctttg
    12721 tggaacggac tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa
    12781 ttctgattac aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg
    12841 ggtgtttaaa tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc
    12901 caatgttgaa ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc
    12961 ttacattgat aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac
    13021 tatcagtact aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa
    13081 cagccaaata gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa
    13141 taatactcct ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac
    13201 aacaaaaggt attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag
    13261 taaaactgta acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa
    13321 tgcaaatttc attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc
    13381 agcaaccatt actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca
    13441 ggcatgggca gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc
    13501 ccccaaagct acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat
    13561 taatcgtcta gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac
    13621 aacccttaca gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct
    13681 ggcccaagca gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca acctcctgtt
    13741 agtcgacttc attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc
    13801 accaaaagga actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga
    13861 tgtaacaccg gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg
    13921 ctataaatct gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg
    13981 ctataaagcc ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga
    14041 cattaacggt gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg
    14101 tggattcagt gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga
    14161 tggccttgac ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga
    14221 tgccttcagt ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga
    14281 gtccggagca tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc
    14341 aactttcgcc ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga
    14401 agtcggttac cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt
    14461 ttacggtgct gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat
    14521 gaaggataag gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt
    14581 ctccggtaag gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt
    14641 tgccaacggt gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa
    14701 agacggtcgt atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt
    14761 cggtcttgag tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc
    14821 caacttccgt tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg
    14881 gagagaatat gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DADLZM010000101        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLZM010000101 DADLZM010000000
VERSION     DADLZM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.102"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="JNY73_002900"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="JNY73_002900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY8048843.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="JNY73_002901"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="JNY73_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY8048844.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHEPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNY73_002902"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNY73_002902"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY8048845.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /locus_tag="JNY73_002903"
     CDS             complement(3965..5611)
                     /locus_tag="JNY73_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY8048846.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="JNY73_002904"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="JNY73_002904"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY8048847.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIKSDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="JNY73_002905"
     CDS             7086..8594
                     /locus_tag="JNY73_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY8048848.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JNY73_002906"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JNY73_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY8048849.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JNY73_002907"
                     /pseudo
     CDS             complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JNY73_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JNY73_002908"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JNY73_002908"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY8048850.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..14283)
                     /locus_tag="JNY73_002909"
     CDS             complement(13693..14283)
                     /locus_tag="JNY73_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8048851.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14512..>14685)
                     /locus_tag="JNY73_002910"
     CDS             complement(14512..>14685)
                     /locus_tag="JNY73_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8048852.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3685 a         3819 c         3496 g         3685 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaacccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattaagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagtgata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctatctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatcg aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcgg gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gaacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
    13861 ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    13921 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    13981 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    14041 aatcccttca atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    14101 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccag gactaatggc
    14161 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    14221 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    14281 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    14341 tcattgctgt tgaatagtgc gaaggtacac tctattcgta tacttttcac ccagcaattg
    14401 gttaaccaat tgccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac
    14461 acgctatccc ggtaaacaac acatacgacc gtttaccggt tcgttagtgt attaattgag
    14521 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
    14581 cgagcaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    14641 cgtgtgctgt aacatttttt gttcaatttc tgggtaatga ctcca
//
LOCUS       DADLZM010000102        14652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLZM010000102 DADLZM010000000
VERSION     DADLZM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.103"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(88..2064)
                     /gene="speA"
                     /locus_tag="JNY73_002911"
     CDS             complement(88..2064)
                     /gene="speA"
                     /locus_tag="JNY73_002911"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY8048853.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENALPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2073..2204)
                     /gene="yqgB"
                     /locus_tag="JNY73_002912"
     CDS             complement(2073..2204)
                     /gene="yqgB"
                     /locus_tag="JNY73_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY8048854.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2340..2555
                     /locus_tag="JNY73_002913"
     CDS             2340..2555
                     /locus_tag="JNY73_002913"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048855.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2859..4013
                     /gene="metK"
                     /locus_tag="JNY73_002914"
     CDS             2859..4013
                     /gene="metK"
                     /locus_tag="JNY73_002914"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY8048856.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4450..5844
                     /locus_tag="JNY73_002915"
     CDS             4450..5844
                     /locus_tag="JNY73_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY8048857.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5921..6418
                     /locus_tag="JNY73_002916"
     CDS             5921..6418
                     /locus_tag="JNY73_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY8048858.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6513..7220
                     /gene="endA"
                     /locus_tag="JNY73_002917"
     CDS             6513..7220
                     /gene="endA"
                     /locus_tag="JNY73_002917"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY8048859.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7300..8031
                     /gene="rsmE"
                     /locus_tag="JNY73_002918"
     CDS             7300..8031
                     /gene="rsmE"
                     /locus_tag="JNY73_002918"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY8048860.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8044..8994
                     /gene="gshB"
                     /locus_tag="JNY73_002919"
     CDS             8044..8994
                     /gene="gshB"
                     /locus_tag="JNY73_002919"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY8048861.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9031..9666
                     /locus_tag="JNY73_002920"
     CDS             9031..9666
                     /locus_tag="JNY73_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY8048862.1"
                     /translation="MTAPFFPRILAAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9666..10082
                     /gene="ruvX"
                     /locus_tag="JNY73_002921"
     CDS             9666..10082
                     /gene="ruvX"
                     /locus_tag="JNY73_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY8048863.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10258..11238)
                     /locus_tag="JNY73_002922"
     CDS             complement(10258..11238)
                     /locus_tag="JNY73_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY8048864.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11256..11960
                     /gene="yggS"
                     /locus_tag="JNY73_002923"
     CDS             11256..11960
                     /gene="yggS"
                     /locus_tag="JNY73_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY8048865.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11978..12544
                     /gene="yggT"
                     /locus_tag="JNY73_002924"
     CDS             11978..12544
                     /gene="yggT"
                     /locus_tag="JNY73_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY8048866.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12541..12831
                     /locus_tag="JNY73_002925"
     CDS             12541..12831
                     /locus_tag="JNY73_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY8048867.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12839..13432
                     /locus_tag="JNY73_002926"
     CDS             12839..13432
                     /locus_tag="JNY73_002926"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY8048868.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAEMTREEKSAISHRGQALKLLLDALRNG"
     gene            13425..14561
                     /gene="hemW"
                     /locus_tag="JNY73_002927"
     CDS             13425..14561
                     /gene="hemW"
                     /locus_tag="JNY73_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY8048869.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGMQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3542 a         3871 c         3754 g         3485 t
ORIGIN      
        1 aagcagctag attattttta agaatatata aaaagaaagc gggccgattt acgtaaataa
       61 atcgacccgc cagcggaata atcttaatta ttcatcttca agataagtat aaccgtacaa
      121 acctgcctcg aactcttcaa ggaactgttg ttgcagttca gcatcaagat cggttttctt
      181 cacttgatcg cggaactggg ttaacagcgt tttcggatcg agctgtacat attgcagcat
      241 gtccgccacg gtatcgcctt cgtcagacag ttctacttct acgctaccgt cagggaacac
      301 gaacacgtca actgcttcgg tatcaccgaa caggttgtgc atattgccaa ggatctcctg
      361 atatgcaccg accataaaga aaccgagcat cggcggattc tctggatcgt actccggcat
      421 tggcattgtc gtggcaatac catcaccatc aatatagtgg tcgatagcac cgtcagagtc
      481 acaggtaata tccagcagca cagcgcgacg ttccggcact tgatccagcc cttccagcgg
      541 tagaaccggg aacaactggt cgatccccca tgcgtccggc attgactgga acagcgagaa
      601 attgacgtac attttgtccg ccatacgttc ctgcagttcg tcgattatcg gacgatgcgc
      661 acggttttgc ggatccagtt gcttctgcac ttcatggcac atgctcagat agagttgttc
      721 tgcccatgca cgttcttgca ggctaaaggt gccggaagag tagccgatat gaatgtcgtg
      781 cagatccatc tggctgtcgt gtaaccattc acgcaaagaa cggcgagttc ccggttcgtg
      841 catttcctgc caggtttccc acatgctttg cagcgcacgc ggcgcatctt ctgcgggcgc
      901 ggtcggcacc gtatattcgt tacgttccac gccgatgata ttcgacacca gcacggtatg
      961 gtgcgcagtc accgcacggc ccgattcggt gattaccgtc ggatgcggca gagcattttc
     1021 ttcacacgca tcgccaatcg cccagatgat gttgttggcg tattcattga ggccatagtt
     1081 tacggagcag tcagactgcg aacgggtgcc ttcataatcc acgcccagac cgccgccgac
     1141 gtcgaagcac tgaatattga cgcccagctt gtgcagttct acatagaaac gcgcggattc
     1201 acgaacgcct gtcgcgatat cgcgaatatt cgccatctgc gaaccgaggt ggaagtgcag
     1261 tagttgcagg ctgtcgagac gcccggcttc acgcagggtt tcaaccagtt gcagtacctg
     1321 agtcgcagcc aggccgaact tcgatttttc cccgccggag gactgccatt tacccgagcc
     1381 ctgcgaagcc agacgtgcac gcacgcccag acgaggaacg acattcagac gttctgcttc
     1441 atccagcaca atggcgattt ctgacatctt ctcaatgacc agatagacct tgtgccccat
     1501 cttctcgcca attaatgcca ggcggatata ttcgcggtct ttataaccgt tgcagacgat
     1561 gacactacga gtcatgccag catgtgccag cactgccatc aactcggctt tggaaccggc
     1621 ttccagaccc agcggttcac ccgaatgaat cagggactcg atcacgcggc ggtgctggtt
     1681 aactttgatc ggataaacaa ggaagtaatc gccgttatag ccgtaggatt ccctcgcacg
     1741 tttgaacgcg gcgttaatgg aacgcaaacg gtgctgcagg atctgtggga aacagaacag
     1801 tgcaggcaga cgctgaccct gtgcttcacg agttttcact aactgcgcga gatcgacgcg
     1861 agcttccggg acgtccgggt ccgggcacac gctgatgtgg cccagctcgt taacgtcata
     1921 gtagttattg ccccaccagg caatattgta agtacgcagc atcttgctgg cttcctggga
     1981 gctcattgca acctcctgca tggagcgtag tacaccgtgt tcgcccgctg acgaaggcaa
     2041 acccatagac atgtcgtcag acatagcgaa cctcaaatta ttttattaag tgtaaaacag
     2101 ttaacgactg tcgcagcctg gaactgcgat aacaacccat aagcacatgg attttccagc
     2161 agtgtatgct gacgctccga ctgcgcaacc ggtttctttt tcataacatt attaagcaca
     2221 taaccgaacg taagtgtgac agttcggcga aaccacgaga aaactcttgt ttttacaaga
     2281 gcgcccttgt tcagtcctca gtaactgtaa ccagctcttg aatcctgaga agcgccgaga
     2341 tgggtataac atcggcaggt atgcaaagca gagatgcaga gtgcggggaa cgaatcttca
     2401 ccagaacggt gagacaggtt aagcagcaga caacggttca ttatttcgta tcacctccac
     2461 ggccgcctgt taagacgaac ccacaagcca aaactctgat ttcaacccgg ctggaagtgg
     2521 caacacgaaa aaaacgtcgt gtgcttttta tttaagccgc gcgccgcgtt ttatacccca
     2581 caatggcaga aaattgcaaa agataaatac gcagaatgcc ggcattgtca ggaaaaattt
     2641 ccagccacgt ttttaacaga atgagacacg attcaaaaaa aagtggaaat cgggtgaaga
     2701 attgacctaa aatagccatc cagatgttaa tccatccata ccgattaaca ctcagactgc
     2761 cagtgttttt aacctgcaga gtcgtggtag gatccgctac cacagaaaat ccacacaaca
     2821 gtttgagcta accaaattct ctttaggtga tattaaatat ggcaaaacac ctttttacgt
     2881 ccgagtccgt ctctgaaggg catcctgaca aaattgctga ccaaatctct gatgccgttt
     2941 tagacgcgat cctcgaacag gatccgaaag cacgcgttgc ttgcgaaacc tacgtaaaaa
     3001 ccggcatggt tttagttggc ggcgaaatca ccaccagcgc ctgggtagac atcgaagaga
     3061 tcacccgtaa caccgttcgc gaaattggct atgtgcattc cgacatgggc tttgacgcta
     3121 actcctgtgc ggttctgagc gctatcggca aacagtctcc tgacatcaac cagggcgttg
     3181 accgtgccga tccgctggaa cagggcgcgg gtgaccaggg tctgatgttt ggctacgcaa
     3241 ctaatgaaac cgacgtgctg atgccagcac ctatcaccta tgcacaccgt ctggtacagc
     3301 gtcaggctga agtgcgtaaa aacggcactc tgccgtggct gcgcccggac gcgaaaagcc
     3361 aggtgacttt ccagtatgac gacggcaaaa tcgttggtat tgatgctgtc gtgctttcca
     3421 ctcagcactc tgaagagatc gaccagaaat cgctgcaaga agcggtaatg gaagagatca
     3481 tcaagccaat tctgcccgct gaatggctga cttctgccac caaattcttc atcaacccga
     3541 ccggtcgttt cgttatcggt ggcccaatgg gtgactgcgg tctgactggt cgtaaaatta
     3601 tcgttgatac ctacggcggc atggcgcgtc acggtggcgg tgcattctct ggtaaagatc
     3661 catcaaaagt ggaccgttcc gcagcctacg cagcacgtta tgtcgcgaaa aacatcgttg
     3721 ctgctggcct ggccgatcgt tgtgaaattc aggtttccta cgcaatcggc gtggctgaac
     3781 cgacctccat catggtagaa actttcggta ctgagaaagt gccttctgaa caactgactc
     3841 tgctggtacg tgagttcttc gacctgcgcc catacggtct gattcagatg ctggatctgc
     3901 tgcacccgat ctacaaagaa accgcagcat acggtcactt tggtcgtgaa catttcccgt
     3961 gggaaaaaac cgacaaagcg cagctgctgc gcgatgctgc cggtctgaag taatctttct
     4021 tcacctgcgt tcaaaggcca gcctcgcgct ggcctttttc ttttacctca ccgcggtgga
     4081 taggcgttca cgccgcatcc agcaaaaaaa ctgcccgcac aataacatca ttcttcctga
     4141 tcacgtttca cggcaaatta ccatcacaat tgaaaccgat tacaccaacc acaccagcaa
     4201 aatatttgta atattttcat attattattc agctttcaca gttgttacat ttcttttcag
     4261 taaagtctta attgcagatg acagcgttta atctatgatg atataactca attattttca
     4321 tgcacttaaa tcataactaa gataaatgtt agtgtaagcg attacactga tgtgatttgc
     4381 ttcacatctt tttacgtcgt actcacctat cttaattcac aataaaaaat aaccatattg
     4441 gagggcatca tgcctgacgc taaaaaacag gggcggtcaa acaaggcaat gacgtttttc
     4501 gtctgcttcc ttgccgctct ggcgggatta ctctttggcc tggatatcgg tgtaattgct
     4561 ggcgcactgc cgtttattgc agatgaattc cagataactt cgcacacgca agaatgggtc
     4621 gtaagctcca tgatgttcgg tgcggcagtc ggtgcggtgg gcatcggctg gctctccttt
     4681 aaactcgggc gcaaaaagag cctgatgatc ggcgcaatcc tgtttgttgc cggttcactg
     4741 ttctctgcgg ctgcgccaaa cgttgaagta ctgattcttt cgcgcgttct gctggggctg
     4801 gcggtgggtg tggcctctta taccgcacca ctgtacctgt cagaaattgc gccggaaaaa
     4861 attcgcggca gtatgatctc gatgtatcag ttgatgatca ctatcgggat cctcggtgct
     4921 tatctttctg ataccgcctt cagctacacc ggtgcatggc gctggatgct gggtgtgatt
     4981 atcatcccgg caattttgct gctgattggt gtcttcttcc tgccagacag cccacgttgg
     5041 tttgccgcca aacgccgttt tgttgatgcc gaacgcgtgc tgctacgcct gcgtgacacc
     5101 agcgcggaag cgaaacgcga actggatgaa atccgtgaaa gtttgcaggt taaacagagt
     5161 ggctgggcgc tgtttaaaga gaatagcaac ttccgccgcg cggtgttcct tggcgtactg
     5221 ttacaggtaa tgcagcaatt caccgggatg aacgtcatca tgtattacgc gccgaaaatc
     5281 ttcgaactgg cgggttatac caacaccacc gagcaaatgt gggggacagt gattgtcggc
     5341 ctgaccaacg tacttgccac ctttatcgca atcggccttg ttgaccgctg gggacgtaaa
     5401 ccaacgctaa cgctgggctt cctggtgatg gctgctggca tgggcgtact cggtacaatg
     5461 atgcatatcg gtatccactc tccgtcggcg cagtatttcg ccatcgccat gctgctgatg
     5521 tttattgtcg gttttgccat gagtgccggt ccgctgattt gggtactgtg ctccgaaatt
     5581 cagccgctga aaggccgcga ttttggcatc acctgctcca ccgccaccaa ctggattgcc
     5641 aacatgatcg ttggcgcaac gttcctgacc atgctcaaca cgctgggtaa cgccaacacc
     5701 ttctgggtgt atgcggctct gaacgtactg tttatcctgc tgacattgtg gctggtaccg
     5761 gaaaccaaac acgtttcgct ggaacatatt gaacgtaatc tgatgaaagg tcgtaaactg
     5821 cgcgaaatcg gtgctcacga ttaatctcct caggcttcct cccgtcgcgg aggaagccgc
     5881 ctcttgcagt catcttttct tcgctctatc ctctgccgct atgaaaacat cccgtctccc
     5941 tatagccatc caacaggccg ttatgcgccg cctgcgggaa aaactcgccc aggccaacct
     6001 gaagctaggg cgtaactacc cggagccaaa actctcttac acccagcgcg gaacctccgc
     6061 cggaacagcc tggctggaaa gctatgaaat ccgcctcaat cccgttttgc tgttggaaaa
     6121 cagtgaagct tttattgaag aagtggtacc gcacgaactg gcacatttgc tggtatggaa
     6181 acatttcggc cgcgtagcgc cacatggcaa agagtggaag tggatgatgg aaagcgtgct
     6241 gggcgttccc gcccgtcgta cgcatcagtt cgaactgcaa tccgtgcgtc gcaacacctt
     6301 cccctaccgc tgtaagtgcc aggaacatca gcttaccgta cgccgccata atcgcgtagt
     6361 tcgtggcgag gccatctatc gctgtgttca ctgcggtgaa cagctggttg cgaaataacc
     6421 acctgaacta tcaggaactt tcctgatctg gctgattgca taccaaaaca actttcgcta
     6481 cgttgctggc tcgttttaac acggagtaag tgatgtaccg ttatttgtct attgctgcgg
     6541 tggtactgag cgcagcattt tccggcccga cgttggccga aggtatcaat agtttttctc
     6601 aggcgaaagc cgcggcggta aaagtccacg ctgatgcgcc cggtacgttt tattgcggat
     6661 gtaaaattaa ctggcagggc aaaaaaggcg ttgttgatct gcaatcgtgc ggctatcagg
     6721 tgcgcaaaaa tgaaaaccgc gccagccgcg tagagtggga acacgtcgtt cccgcctggc
     6781 aattcggtca ccagcgccag tgctggcagg acggtggacg taaaaactgc gctaaagatc
     6841 cggtctatcg caagatggaa agcgatatgc ataacctgca gccgtcagtc ggcgaggtga
     6901 atggcgatcg cggcaatttt atgtacagcc agtggaatgg cggcgaaggc cagtacggtc
     6961 aatgcgccat gaaggtcgat ttcaaagaaa aagctgccga accaccagcg cgtgcacgcg
     7021 gtgccattgc gcgcacctac ttctatatgc gcgaccaata caacctgaca ctctctcgcc
     7081 agcaaacgca gctgttcaac gcatggaaca agatgtatcc ggttaccgac tgggagtgcg
     7141 agcgcgatga acgcatcgcg aaggtgcagg gcaatcataa cccgtatgtg caacgcgctt
     7201 gccaggcgcg aaagagctaa cctacactag cgggattctt tttgttaacc cctaccccac
     7261 gcgtacaacc gcgtggggag acgacgcgga tttttaacta tgcgtatccc ccgcatttat
     7321 catcctgaac cactgaccag ccattctcac atcgcgcttt gcgaagatgc cgccaaccat
     7381 atcgggcgcg tactgcgcat ggggccgggg caggcgttgc aattgtttga cggtagcaac
     7441 caggtctttg acgccgaaat taccagcgcc agcaaaaaaa gcgtggaagt gaaggtgctg
     7501 gaaggccaga tcgacgatcg cgaatctccg ctgcatattc acctcggtca ggtgatgtcg
     7561 cgtggtgaaa aaatggaatt tactatccag aaatcgatcg aactcggtgt aagcctcatt
     7621 acgccacttt tttctgagcg ctgcggcgtt aaactggata gtgaacgtct gaacaagaag
     7681 cttcagcagt ggcagaagat tgcaattgct gcctgtgagc agtgtggtcg taaccgggtg
     7741 ccggaaatcc gtccagcgat ggatctggaa gcctggtgtg cagagcagga tgaaggactg
     7801 aaactgaatc ttcacccgcg cgccagtaac agcatcaata cgttgccttt accggttgaa
     7861 cgcgtccgcc tgctgattgg cccggaaggc ggtttatcgg cagatgaaat tgccatgact
     7921 gcccgctatc aatttactga tatcctgttg ggacctcgcg ttttgcgtac agagacaact
     7981 gcgctcaccg ccattaccgc gctacaagta cgatttggcg atttgggcta acggagaaga
     8041 ataatgatca agctcggcat cgtgatggac cccatcgcaa acatcaacat caagaaagat
     8101 tccagtttcg ctatgttgct ggaagcacag cgtcgtggtt acgaacttca ctatatggag
     8161 atgggcgatc tgtatctgat caatggcgaa gcccgcgccc atacccgcac gctgaacgtg
     8221 aagcagaact acgaagagtg gttttcgttc gtcggtgaac aggatctgcc gctggccgat
     8281 ctcgatgtga tcctgatgcg taaagacccg ccgtttgata ccgagtttat ctacgcgacc
     8341 tatattctgg aacgtgccga agagaaaggg acgctgatcg ttaacaagcc gcagagcctg
     8401 cgcgactgta acgagaaact gtttaccgcc tggttctctg acttaacgcc agaaacgctg
     8461 gttacgcgca ataaagcgca gctaaaagcg ttctgggaga aacacagcga catcattctt
     8521 aagccgctgg acggtatggg cggcgcgtcg attttccgcg tgaaagaagg cgatccaaac
     8581 ctcggcgtga ttgccgaaac cctgactgag catggcactc gctactgcat ggcgcaaaat
     8641 tacctgccag ccattaaaga tggcgacaaa cgcgtgctgg tggtggatgg cgagccggta
     8701 ccgtactgcc tggcgcgtat tccgcagggg ggcgaaaccc gtggcaatct ggctgccggt
     8761 ggtcgcggtg aacctcgtcc gctgacggaa agtgactgga aaatcgcccg tcagatcggg
     8821 ccgacgctga aagaaaaagg gctgattttt gttggtctgg atatcatcgg cgaccgtctg
     8881 actgaaatta acgtcaccag cccaacctgt attcgtgaga ttgaagcaga gtttccggtg
     8941 tcgatcaccg gaatgttaat ggatgccatc gaagcacgtt tacagcagca gtaacccacc
     9001 ttagcgagaa ggatctcgtt gagactctga gtgacagcgc ccttctttcc acgcatactg
     9061 gccgctgttg cttttttgaa ccaggaaaca gaacctctga caatgaattt acagcatcac
     9121 tttcttattg ccatgcctgc tctccaggat ccgattttcc gtcgttccgt ggtctacatt
     9181 tgcgaacata ataccaatgg tgcaatgggg atcatcgtca acaagccgct ggaaaatctc
     9241 aaaattgaag ggattctgga aaagctgaag atcacgccgg agccgcgtga tgaatcaatc
     9301 cgtctggata aaccggttat gctcggcggc ccactggctg aagatcgcgg gtttattttg
     9361 catactccgc cctccaattt tgcttccagc attcgcattt cagacaacac ggtgatgacc
     9421 acttcccgcg atgtgctgga aacgctcggc accgataaac aaccgtctga cgtattggtg
     9481 gctctgggct atgcctcctg ggaaaaaggt caactggaac aagaaattct cgataacgcg
     9541 tggctaacgg ccccggcgga tctgaatatt ctgttcaaaa cgccgattgc cgaccgctgg
     9601 cgcgaggcgg caaaactgat tggtgtggat attctcacca tgcctggtgt ggcaggacac
     9661 gcctgatgag tggaacctta ctcgccttcg acttcggcac caaaagcatt ggcgtagcgg
     9721 tcggccaacg cattaccggc accgctcgcc ctttgcctgc aattaaagcg caggacggta
     9781 cgccggactg gaacctcatc gaacgtttac tgaaagagtg gcagcctgac gaaatcatcg
     9841 tcggtttgcc gctgaatatg gacggcaccg aacagccgtt aaccgccaga gcgcgcaaat
     9901 ttgccaaccg tattcatggt cgtttcggtg ttgaagtaaa gctccatgac gagcgtctta
     9961 gcactgtgga agcccgttcc ggtctttttg aacagggcgg ctatcgggcg ctcaacaaag
    10021 gcaaagttga ctctgcctct gcggttatta ttctcgaaag ctatttcgag cagggatatt
    10081 aaggcgatct aaaagcctgg cggatagtcg ttaccgccat cttgccggat gcggcgtgaa
    10141 cgccttatcc agcacacatc tggcagcggc tgtaggtctg ataagacgcg ttagcgtcgc
    10201 atcagacatt tattgccttt ataggcccta taaacttgcg catcgggcat ggcaacgtca
    10261 caaacgccct tcccccaccc gctgctgata actctgctga aacgttaaca tccccacctg
    10321 ctgcccggtt tgaataacat gcggtaactg gtgggttttc ccttcgcgaa tcaaattccc
    10381 caccgcggga gtgttaatca gtaattcaaa cagcgccacg cgtccttcct gtttatccac
    10441 ttccagcttt tgtgacagca ctgcccgtaa actacccgca agttgattac gtacgggatc
    10501 tttttcctgc gccggaaatg aatccaccag tcgctcaact gcctgcgcgg caccacgtgt
    10561 atgtaaggtt gccagcacca gatgcccggt ttctgccgcc gtcagcgcca gacggattgt
    10621 ctcactgtca cgtagctctc cgagcaaaat cacatcagga tcttcccgca atgcagcccg
    10681 caaccccgat gcgaacgtca tacagtgcaa accaatttcc cgctgctgga tcaaacatcg
    10741 ctggctggca tagagatatt ccacaggatc ttccagcgtc agaatatgcg catcggcatg
    10801 ttgattaaga tagccaacca tcgccgccag cgtggtagat ttgccactcc ccgtagcccc
    10861 cgtcaccaga atcaggccat tctcgctctt gagtaattcc ggcaataccg gtggcgcacc
    10921 aagctgttcg agctgtggac agtgcgaagg taacaaccgt aatgccagag aaataccttg
    10981 ccgttgcgcg aacgcactgc cacgcaaccg ctggttttcc gccagcgaca cggcaaaatc
    11041 cagctggcca ttttccagca atattgcccg ctgatcgtca tccagccact cccgcagtag
    11101 ctcttcgacg tccggcgcat caaacggcgc agcttccatt ctgccacgaa tgcgccatcg
    11161 tgcgggccag gcgctgcaca ggtgtagatc cgagacgtta tgctttacac taagggccac
    11221 aatttcttcc atattcatac taagatcctc ggaaaatgaa cgatattgcg cataacctgg
    11281 cacaggtccg ggacaaaatc tcagcggctg caacgcgttg cggccgttct ccagaagaaa
    11341 ttacgctgct tgcagtcagt aaaacaaaac ctgcgagcgc catcgcagaa gccattgatg
    11401 ccgggcagcg tcaatttggt gaaaactacg ttcaggaagg ggtagataaa attcgccact
    11461 ttcaggaact gggcgtaaca ggattagaat ggcattttat tggcccgttg cagtctaata
    11521 aaagccgcct agtggcagag catttcgact ggtgtcatac catcgaccgt ttgcgcattg
    11581 ctacccgcct caacgatcag cgcccggcag aacttccccc tcttaacgta ctgattcaaa
    11641 ttaatattag tgatgaaaac agtaagtccg ggattcaact ggcagaactg gacgagctgg
    11701 cagcggcggt cgctgaacta ccgcgtttac gtctgcgcgg gctgatggca atccctgcgc
    11761 ctgagtcaga atatgtaagg cagtttgaag ttgcacgcca aatggctgta gcatttgccg
    11821 gactgaaaac gcgctacccg catatcgaca cgctctctct gggaatgtcg gacgatatgg
    11881 aagccgccat tgcggcaggt agcacgatgg ttcgtatcgg cactgcaatt tttggtgcgc
    11941 gtgattactc taaaaaataa ggaattaaag gaacgccatg aatacgttga ctttcctgct
    12001 ttcaacggtc attgagctgt ataccatggt gctgttatta cgcatctgga tgcagtgggc
    12061 tcattgtgat ttttacaacc ccttctcaca gtttgtagtg aaggtagcgc agccaattat
    12121 cgggccactg cgccgcgtta ttccggcaat gggtcccatt gacagcgcct cgctgctggt
    12181 tgcctatatt ctcagtttta tcaaagccat tgtgctgttt gaagtggtga ccttcctgcc
    12241 aatcatctgg attgccggtt tactgattct gctgaaaacc atcggcttgc tgattttctg
    12301 ggtcctgctg gtgatggcga ttatgagctg ggtaagccag ggtcgtagcc cgattgaata
    12361 cgtgctgatt cagctggccg atccgctgct gcgcccgatt cgccgcttgc taccggcaat
    12421 gggtgggatt gatttctcgc cgatgatcct cgttctgctg ctgtatgtca tcaatatggg
    12481 tgtcgcagaa gtattacagg caaccggaaa catgctgctg ccggggctgt ggatggcgtt
    12541 atgagtgccg taacagttaa tgatgacggt ctggttttac ggctctatat tcagccgaaa
    12601 gccagccgtg attctattgt cggtttacat ggcgacgaag ttaaagtcgc cattaccgcg
    12661 ccgccggttg acggccaggc caacagtcat ctggtgaagt ttctcggtaa gcaattccgg
    12721 gttgccaaaa gccaggtggt gattgaaaaa ggcgaacttg gccgccacaa acaaattaaa
    12781 atcattaatc cgcaacaaat cccgccagaa atcgcggcgt taattaatta ggtatcctat
    12841 gcaaaaagtt gtcctcgcaa ccggcaatgc cggtaaagtg cgtgagctgg cgtcgctgct
    12901 tagcgacttt ggtcttgata tcgtggccca aacagacctc ggcgttgatt ccgctgaaga
    12961 aaccggccta acctttatcg aaaacgcgat tctgaaagcg cgccatgcgg caaaagtgac
    13021 cggtttaccg gcaattgccg acgactctgg tctggcggta gatgcgctgg gtggcgcgcc
    13081 gggtatttac tccgcgcgtt attccggtga agacgcgacc gatcaaaaga atctgcaaaa
    13141 actgttggaa acactgaaag acgtaccgga cgaccaacgt caggcgcgtt tccactgcgt
    13201 gctggtatat ctgcgtcacg cggaagatcc gactccgctg gtatgccacg gcagctggcc
    13261 gggcgtgatt actcgcgaac cagcgggcac tggtggcttt ggttatgatc caatcttctt
    13321 cgtaccttcc gaagggaaaa ccgccgccga aatgacccgc gaagaaaaga gcgccatttc
    13381 ccaccgtggt caggcgttga aactgctgct ggacgcttta cgtaatggtt aaattaccgc
    13441 cgctgagtct ctacattcac atcccgtggt gcgtgcagaa atgcccgtac tgcgatttca
    13501 actctcacgc gttgaaagga gaagtgccgc acgacgatta tgttcagcat ctgctttgcg
    13561 atctggacaa tgatgtggct tacgctcagg gccgtgaagt aaagacaatc tttattggcg
    13621 gtggtacgcc gagcctgctt tccggcccgg cgatgcaaac gctgctagac ggcgtgcgtt
    13681 cgcgtttgcc gctggcagcg gatgcagaaa ttactatgga agcgaaccca ggcacggtag
    13741 aagcggatcg ctttgtcgat tatcagcgtg ctggtgtaaa ccgcatctct attggtatgc
    13801 agagttttag cgaagaaaag ctgaaacgac ttgggcgtat tcatggcccg caagaagcga
    13861 aacgagcggc aaagctggcg agcggtttag ggttacgtag ctttaacctt gatttgatgc
    13921 atggactacc ggatcaatca ctggaagagg cgcttggcga tctacgccag gccattgaac
    13981 tgaatccgcc gcatctttcc tggtatcaac tgaccatcga acctaatacg ctgtttggct
    14041 cgcgaccgcc ggtgctgccg gacgatgacg cgctgtggga tattttcgaa caggggcatc
    14101 agttattaac cgcagcgggt tatcagcaat atgaaacttc cgcttacgcc aaacccggtt
    14161 atcagtgcca gcacaatctc aactactggc gctttggtga ctacatcggt attggctgcg
    14221 gcgcgcacgg caaagtgacc ttcccggatg ggcgcattct gcgtaccact aaaacgcgtc
    14281 atcctcgtgg ttttatgcaa ggaaggtatc tggaaagcca gcgtgatgtc gaagccgcag
    14341 ataagccgtt tgagttcttt atgaatcgct tccgtctgct ggaggccgcg ccgcgcgtgg
    14401 agtttattgc gtataccggg ctttgcgaag atgtgattcg cccacagtta gacgaggcga
    14461 ttgcccaggg ttatctcacc gaatgtgcgg attactggca gataacggaa catggtaaat
    14521 tattcttaaa ctcacttctt gaattattcc tcgctgagta atcattaatg cgcctgcggg
    14581 cgcattaatc acttctttta tccccccgtt aattaacctc ggcagaaata tatttcttac
    14641 tgaataaata tc
//
LOCUS       DADLZM010000103        14473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLZM010000103 DADLZM010000000
VERSION     DADLZM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.104"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..975)
                     /gene="mxiG"
                     /locus_tag="JNY73_002928"
     CDS             complement(<1..975)
                     /gene="mxiG"
                     /locus_tag="JNY73_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY8048870.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQSNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHK"
     gene            complement(984..1442)
                     /gene="ipgF"
                     /locus_tag="JNY73_002929"
     CDS             complement(984..1442)
                     /gene="ipgF"
                     /locus_tag="JNY73_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY8048871.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(1442..1804)
                     /gene="ipgE"
                     /locus_tag="JNY73_002930"
     CDS             complement(1442..1804)
                     /gene="ipgE"
                     /locus_tag="JNY73_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY8048872.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(1814..3430)
                     /locus_tag="JNY73_002931"
     CDS             complement(1814..3430)
                     /locus_tag="JNY73_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY8048873.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVYYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            3744..5228
                     /gene="icsB"
                     /locus_tag="JNY73_002932"
     CDS             3744..5228
                     /gene="icsB"
                     /locus_tag="JNY73_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY8048874.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            5241..5630
                     /gene="ipgA"
                     /locus_tag="JNY73_002933"
     CDS             5241..5630
                     /gene="ipgA"
                     /locus_tag="JNY73_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY8048875.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            5646..6272
                     /gene="ipgB"
                     /locus_tag="JNY73_002934"
     CDS             5646..6272
                     /gene="ipgB"
                     /locus_tag="JNY73_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY8048876.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            6329..6796
                     /gene="ipgC"
                     /locus_tag="JNY73_002935"
     CDS             6329..6796
                     /gene="ipgC"
                     /locus_tag="JNY73_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY8048877.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            6802..8544
                     /gene="ipaB"
                     /locus_tag="JNY73_002936"
     CDS             6802..8544
                     /gene="ipaB"
                     /locus_tag="JNY73_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY8048878.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            8564..9655
                     /gene="ipaC"
                     /locus_tag="JNY73_002937"
     CDS             8564..9655
                     /gene="ipaC"
                     /locus_tag="JNY73_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY8048879.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSI
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSL
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            9706..10704
                     /gene="ipaD"
                     /locus_tag="JNY73_002938"
     CDS             9706..10704
                     /gene="ipaD"
                     /locus_tag="JNY73_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY8048880.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRK
                     GYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            10713..12614
                     /gene="ipaA"
                     /locus_tag="JNY73_002939"
     CDS             10713..12614
                     /gene="ipaA"
                     /locus_tag="JNY73_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY8048881.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYNAAKDVTTSLS
                     KVLKNINKD"
     gene            12618..12854
                     /locus_tag="JNY73_002940"
     CDS             12618..12854
                     /locus_tag="JNY73_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8048882.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            13071..14000
                     /locus_tag="JNY73_002941"
     CDS             13071..14000
                     /locus_tag="JNY73_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY8048883.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
BASE COUNT         5130 a         2861 c         2204 g         4278 t
ORIGIN      
        1 tttatgttca gaaatcagtc tattaatgag ctcaagcttt tcatctgtca gttcttctct
       61 gacagaatat gtaactttat tattttcaca aatctcttta tactggacat ttacctttgt
      121 gaaaatacct ttagcatccc cccttgcatt atgatcgcta agatagttga atttaatatt
      181 tttgatatac ggaaattcaa caatcagagc atttttcaat ttatcaagtt cggtatcttt
      241 tgaactattt ctttctttac ttaaaaacac cagtaattcc gctttatcac tcactaaatt
      301 caacacatac aaattgataa atgggaatcg ttgattgata tattgttgaa ttctatttat
      361 ttctttgttt gacaccagaa tatatctgca tttggtaaat ccggttttat ttaaggctac
      421 agacgcccat accgcatcat tttgtgtttc ggcaagaata taattgcact gtccagtgtt
      481 gactattcca tatctcttat catctataat ttcagcaatt tcttttactt catctttctt
      541 taaagagagg gaaaaaatta taattaacac aacaataaca gcaaagaaaa aaaacaccga
      601 gtggttctta tacattccgt ttagtatatg gcctctcgat ttatcctccc tcatattttt
      661 aaggtgaaaa gaaatcccgt catattcaaa tgaattagat tgtgaattga tatcaatttg
      721 ctcacctttt tcatcactaa attgacagat attatctttg cttatggtca aataaattat
      781 accatccgat ttactatctg ttacaggaat aatattttct gggaaattgt cgtttccaaa
      841 ctcaagtgtt tctatcgtcc gtccgagaac aattaagtta ttcccataat ataacggaaa
      901 ctcatccccg acaccgtttg tgagtttcac taataacctg aatggtgcaa gatttgagtt
      961 ctttgcctca gacataaatc ctcttatatc cttcgattat tctgcttgct ctctgctgct
     1021 atccgcaaat agcgtctata gatatcctct gcgtacttta atctttcttt tttctttttc
     1081 ggagacgttc ctgcattata ggctccaaca gcctcccacc ctcttccata catcatcata
     1141 aactcattta ataactttgc tgcataatgt acagaaagac atggatgact aattagcatt
     1201 tcctcagaat atcccatttc tctaagtctt ttagaatgaa agtcatttat ttgcattata
     1261 ccataatctt tacttccatt gttattaaca ttgactgcag atttattaaa tccggattct
     1321 ttttccgcaa tcgcttttaa caaacttgat ggaatattgt acctttcacc agccttatcc
     1381 caacaatcag ctctccctaa atatgggata aaacacagca aaataaatac gaaacgggac
     1441 attaataccc cttcattctt cgcgcaaatt catccttcag ccaacgcaca cgcgaaatat
     1501 aatattctgt attaacatat acatcctcga attcatttat cccggtaaga tctaatcgcg
     1561 caattagatt tccgccttca tcatcagtag ctaagcatat cttttctgag taatttaggc
     1621 tcaacgcata tataagatca tttatatttt caggcaatgc acaaaaaggg cataataaat
     1681 ttattgaatc tccttctttt tcaatatata ttagcacatc atcatcaagc atgattgctt
     1741 gatcatcgat atcaattaaa gggataccca aggctcggca aataacatct gctaaatctt
     1801 ccatatattc ctcttataca aatgacgaat accctttcac catattccat atttttgggt
     1861 cccctattct ttcagaataa gatagctcta acgaagatag cggcaatttt ttcatgactt
     1921 tatttccggg aacaccagta ttcatctctt ggatttccat attaccacta ttcattagaa
     1981 tagtagaaaa taatcttttc tcttctgagg ataatttact atttaattga gaaaactgac
     2041 ctgtttcatg ttttcttatt atttctcttt taatttcagc atcctgcata cctgttctgt
     2101 ccttcccact cttacaattc cagcaaggta cagcacctat agtataagcc aatagagtca
     2161 ttctttgtga caatttatac ggctctccat tatcattttt ttgtaacttc ttgtttataa
     2221 tctcttttat ctggttagcc agatatatca catcattctt acatggtgga tttttttcta
     2281 ttgcttctgc agcccaacct ccaatcacac catttttgag gaaattatcc cccaacaaag
     2341 aacatattga ttcatcatta agcttgtcaa cattcctcca gcctaagccc atttttagcg
     2401 ctaattcatt tacaccaaag ttaaatgtca ccacttttag attaacgctt acttctttca
     2461 gaagaccatc actattccga attaataatt tcgttggctc ccccctttta gagtttaacc
     2521 cttttaatgc gttcacctga tcttttaaca tactctcctc tcccccggtt aaactggtcg
     2581 gcgtcagaag agaagtcgaa acaatcttta aatctactgt tttaccagac aaagcctgtg
     2641 ataataactc tggtctgcta tataatgctg cgcttactaa ctcctcagct ttattacgag
     2701 cagcaacagc tctttcagat gagttttttt tcaatccata ggctgatata actccatgtc
     2761 tgatacccga aaaaatttcc ttcccctcgt catccaccac ttttgaaagc cacatattcg
     2821 cgatatgctc actctctctc gtagaagcac aacaaattcc ttttccatta tactctttga
     2881 caaatatatt tttattccct atcttcatat gagaggcagg tgttaattga aaaccataat
     2941 ttttcccatg atgactaata ttcttgtcga ccggtcccca attcttatta tttaaataat
     3001 cagtaaatat ttgttttatt ttcgtttgca attccttttt atttttaatg cctagctgtg
     3061 ctcttatctc ttcacttatt actttattaa gctcattaat atgtctttta ttaacttcac
     3121 caagtttata ggtatctaat cgttccatca tggctttaat ttttgacgta tcaccacctg
     3181 ccaactttat tgcctcgtaa taaacttcag ctctgtcacg cgcaaacaaa aggctttttc
     3241 ctgttaagct tgtttggttc tgtaaatata atcgattaag cgcttcgatc cccttatttc
     3301 tttctccatt tttaactctt tgatatgaaa tcacgcttac acctttgtgc tttccggttt
     3361 cttctgcgcc tttataggaa tctccgcttt gaaatgaaac ctgatgcaat cccaaattag
     3421 ttatgtgcat ttaattatcc tcagtcataa atggggcatt gattgcagtt ttgtacctcg
     3481 ttttacaaca aaaaagatac ttattcaaca gggttctctc tttttctata acactccgag
     3541 cctgatacat caggcacttt atcttgtggg atttcatgat gaaacgagca ctacatatgc
     3601 tcacgaggta catattatgt acagcaaaag aaaatttgat tatgttaaca ggctcaagtt
     3661 tctaaattta ttaattgaat atattatatg ccaaatacat tttacatccc cacaatcacc
     3721 aagtaatgga gagttaataa agtatgagcc tcaaaattag caatttcatt gacgcaagca
     3781 atacaaaagg gcctatacgc gttgaagata cagagcatgg acctatattg atagcacaga
     3841 aattcaacct aaaggatctc tttttcagaa cattaagcac tattaatgca aagataaatt
     3901 cacagatatt aaatgaacaa ctaaaaaatt atcgactaga gaatcaaaaa tccttattgc
     3961 ttttcctcaa tacattagct agcgaaaaat ctgcagaaag tgcgtttgcc gcctatgaag
     4021 cagctaaaaa ctcaattcaa cactctttca caggtagaga catcaaatta atgttgaata
     4081 ccgcagagcg ttttcatggc atcggtacag ccaaaaatct tgaaagacat ttagtttttc
     4141 gttgctgggg aaatagaggc ataacccatt tagggcatac tagtatcagt ataaaaaaca
     4201 atttactaca ggaaccaact catacctatc tcagctggta tcccggcggg gatgttacaa
     4261 aagatacaga aataaactac ctttttgaaa aacgcttagg ttacagtgtc gatacctata
     4321 agcaagacaa actaaatatg atttcagacc aaacagccga aagacttgat gcaggccaag
     4381 aggttagaaa cttattaaat tcaaaacaag atcaaaataa caacaaaaaa atattttttc
     4441 ccagagctaa tcaaaaaaaa gacccctatg gttattgggg ggtcagtgct gataaagtat
     4501 acattccatt atcaggtgat aataaaacaa aggatggtaa aatcagccat aacctttttg
     4561 gccttgatga aacaaacatg tcaaaattta tatgcaaaaa gaaagcagat gctttcagac
     4621 aattggcaaa ttataaatta atcagtaaat ctgaaaactg tgctggtatg gcacttaatg
     4681 ttcttaaagc aggaaattct gaaatatact ttccactccc tgacgttaaa ttggttgcta
     4741 cacctaacga tgtatacgcg tatgcaaata aagttaggca acgcattgaa agtctcaatc
     4801 agtcttataa cgaaataatg aagtatattg aatctgattt tgatctttcc agattgactc
     4861 aattacgacg cagctatctt aaaagtttca ataaaattaa ccttatccac acaccaaaaa
     4921 cattcaagcc tttatcaatt tcactttaca aacatcctac tgaaaatgta tcttcagaag
     4981 actttgatgt ggtcatcaac gcttgccact catacttagt aaaatcagca cccagcaata
     5041 tgactcgtgt attaaacgaa ttaaaaaccg aagcaacaga taaaaaagaa gaaattatcg
     5101 aaaaatcaat aaaaattatt gattattaca attctcttaa atcgccagat cttggaacca
     5161 aactttacat tcacgactta ttacagatta ataagctttt attgaataac tctcattcta
     5221 atatatagaa ggccatagaa atgtgtcgca aactatatga taaactttat gaaataacag
     5281 gtgcaaagct tgattttaac gataaaaatc aagcctttat attgctcgaa gaacaaatac
     5341 ctgtttgtat aacagataat gatgaataca tctttttgac cggacttctt aatgaacatg
     5401 aactttttac agaaaatatt attaatccag aacacatact catccttaac tactctttga
     5461 gtagagatta tggttcttct atctgtcttc tccctgatac tcatcaatgt gttttgacca
     5521 agaaacacta caaaaaatat ctttcacctg atgaactcat tgaatctttg tatgaatttt
     5581 tattttgtat taaactaacc attgcaaaca tcacttcaga agtgaactaa catatagggg
     5641 gtatcatgca aattctaaac aaaatacttc cacaggttga attcgcgatt cctcgcccat
     5701 catttgattc gttaagtcac aataagcttg taaagaaaat attatctgtt tttaacttaa
     5761 aacaacgctt tcctcagaag aattttggat gcccggtgaa tataaataaa atcagggaca
     5821 gcgtcataga caaaataaaa gatagtaatt cggggaatca actattttgt tggatgagcc
     5881 aagagcgaac ctcttatgtc tcttcaatga taaacagaag tatagatgaa atggccatac
     5941 acaatggagt tgttttgacc tcagataaca aaaaaaacat atttgcagct attgaaaaaa
     6001 aattcccgga tataaaactt gatgaaaaat cagcacaaac atctatcagt catacagcac
     6061 taaacgagat tgcctcatct ggcctcagag caaagatttt aaaacgctat agcagtgaca
     6121 tggatttgtt taacacacaa atgaaggatc ttacaaatct agtatccagt tccgtctacg
     6181 ataaaatatt taatgaatca acaaaagttt tacaaataga aataagcgca gaggttctaa
     6241 aggctgtata ccgtcaaagc aatacaaatt aaatctttta tattaaatct tatacttatt
     6301 aaagtaaagc agacaaaaag gagaccttat gtctttaaat atcactgaaa atgaaagcat
     6361 ctctactgca gtaattgatg caattaactc tggcgctaca ctgaaagata ttaatgcaat
     6421 tcctgatgat atgatggatg acatttattc atatgcttat gacttttaca acaaaggaag
     6481 aatagaggaa gctgaagttt tcttcagatt tttatgtata tacgactttt acaatgtaga
     6541 ctacattatg ggactcgcag ctatttatca gataaaagaa cagttccaac aagcagcaga
     6601 cctttatgct gtcgcttttg cattaggaaa aaatgactat acaccagtat tccatactgg
     6661 acaatgccag cttcggttga aagccccctt aaaagctaaa gagtgcttcg aactcgtaat
     6721 tcaacacagc aatgatgaaa aattaaaaat aaaagcacaa tcatacttgg acgcaattca
     6781 ggatatcaag gagtaattat tatgcataat gtaagcacca caaccactgg ttttcctctt
     6841 gccaaaatat tggcttccac tgagcttgga gacaatacta tccaagctgc aaatgatgca
     6901 gctaacaaat tattttctct tacaattgct gatcttactg ctaaccaaaa tattaataca
     6961 actaatgcac actcaacttc aaatatatta atccctgaac ttaaagcacc aaagtcatta
     7021 aatgcaagtt cccaactaac gcttttaatt ggaaacctta ttcaaatact cggtgaaaaa
     7081 tctttaactg cattaacaaa taaaattact gcttggaagt cccagcaaca ggcaagacag
     7141 caaaaaaacc tagaattctc cgataaaatt aacactcttc tatctgaaac tgaaggactt
     7201 accagagact atgaaaaaca aattaataaa ctaaaaaacg cagattctaa aataaaagac
     7261 ctagaaaata aaattaacca aattcaaaca agattatccg aactcgaccc agagtcacca
     7321 gaaaagaaaa aattaagccg ggaagaaata caactcacta tcaaaaaaga cgcagcagtt
     7381 aaagacagga cattgattga gcagaaaacc ctgtcaattc atagcaaact tacagataaa
     7441 tcaatgcaac tcgaaaaaga aatagactct ttttctgcat tttcaaacac agcatctgct
     7501 gaacagctat caacccagca gaaatcatta accggacttg ccagtgttac tcaattgatg
     7561 gcaaccttta ttcaactagt tggaaaaaat aatgaagaat ctttaaaaaa tgatctggct
     7621 ctattccagt ctctccaaga atcaagaaaa actgaaatgg agagaaaatc tgatgagtat
     7681 gctgctgaag tacgtaaagc agaagaactc aacagagtaa tgggttgtgt tgggaaaata
     7741 cttggggcac ttttaactat cgttagtgtt gttgcagcag ctttttctgg aggagcctct
     7801 ctagcactgg cagctgttgg tttagctctt atggttacgg atgctatagt acaagcagcg
     7861 accggcaatt ccttcatgga acaagccctg aatccgatca tgaaagcagt cattgaaccc
     7921 ttaatcaaac tcctttcaga tgcatttaca aaaatgctcg aaggcttggg cgtcgactcg
     7981 aaaaaagcca aaatgattgg ctctattctg ggggcaatcg caggcgctct tgtcctagtt
     8041 gcagcagtcg ttctcgtagc cactgttggt aaacaggcag cagcaaaact tgcagagaat
     8101 attggcaaaa taataggtaa aaccctcaca gaccttatac caaagtttct caagaatttt
     8161 tcttctcaac tggacgattt aatcactaat gctgttgcca gattaaataa atttcttggt
     8221 gcagcgggtg atgaagtaat atccaaacaa attatttcca cccatttaaa ccaagcagtt
     8281 ttattaggag aaagtgttaa ctctgccaca caagcgggag gaagtgtcgc ttctgctgtt
     8341 ttccagaaca gcgcgtcgac aaatctagca gacctgacat tatcgaaata tcaagttgaa
     8401 caactgtcaa aatatatcag tgaagcaata gaaaaattcg gccaattgca ggaagtaatt
     8461 gcagatctat tagcctcaat gtccaactct caggctaata gaactgatgt tgcaaaagca
     8521 attttgcaac aaactactgc ttgatacaaa taaggagaat gttatggaaa ttcaaaacac
     8581 aaaaccaacc cagattttat atacagatat atccacaaaa caaactcaaa gttcttccga
     8641 aacacaaaaa tcacaaaatt atcagcagat tgcagcgcat attccactta atgtcggtaa
     8701 aaatcccgta ttaacaacca cattaaatga tgatcaactt ttaaagttat cagagcaggt
     8761 tcagcatgat tcagaaatca ttgctcgcct tactgacaaa aagatgaaag atctttcaga
     8821 gatgagtcac acccttactc cagagaacac tctggatatt tccagtattt cttctaatgc
     8881 tgtttcttta attattagtg tagccgttct actttctgct ctccgcactg cagaaaataa
     8941 attgggctct caattgtcat tgattgcgtt cgatgctaca aaatcagctg cagagaacat
     9001 tgttcggcaa ggcctggcag ccctatcatc aagcattact ggagcagtca cacaagtagg
     9061 tataacgggt atcggtgcca aaaaaacgca ttcagggatt agcgaccaaa aaggagcctt
     9121 aagaaagaac cttgccactg ctcaatctct tgaaaaagag cttgcaggtt ctaaattagg
     9181 gttaaataaa caaatagata caaatatcac ctcactacaa actaactcta gcacaaaatt
     9241 tttaggtaaa aataaactgg cgccagataa tatatccctg tcaactgaac ataaaacttc
     9301 tcttagttct cccgatattt ctttgcagga taaaattgac acccagagaa gaacttacga
     9361 gctcaatacc ctttctgcgc agcaaaaaca aaacattggc cgtgcaacaa tggaaacatc
     9421 agccgttgct ggtaatatat ccacatcagg agggcgttat gcatctgctc ttgaagaaga
     9481 agaacaacta atcagtcagg ccagcagtaa acaagcagag gaagcatccc aagtatctaa
     9541 agaagcatcc caagcgacaa atcaattaat acaaaaatta ttgaatataa ttgacagcat
     9601 cacccaatca aggaattcgg cagccagtca gattgctggt aacattcgag cttaatatat
     9661 ccaagagcca taataatata tggctcttcc tgtaaggaaa taaccatgaa tataacaact
     9721 ctgactaata gtatttccac ctcatcattc agtccaaaca ataccaacgg ttcatcaacc
     9781 gaaacagtta attctgatat aaaaacaacg accagttctc atcctgtaag ttcccttact
     9841 atgcttaacg acacccttca taatatcaga acaacaaata aggcattaaa gaaagagctt
     9901 tcacaaaaaa cgttgactaa aacatcgcta gaagaaatag cattacattc atctcagatt
     9961 agcatggatg taaataaatc cgctcaacta ttggatattc tttccaggaa aggatatcca
    10021 attaataaag acgcaagaga attattacat tcagccccga aagaagccga gcttgatgga
    10081 gatcaaatga tatctcatag agaactgtgg gctaaaattg caaactccat caatgatatt
    10141 aatgaacagt atctgaaagt atatgaacat gccgttagtt catatactca aatgtatcaa
    10201 gattttagcg ctgttctttc cagtcttgcc ggctggatct ctcccggagg taacgacgga
    10261 aactccgtga aattacaagt caactcgctt aaaaaggcat tggaagaact caaggaaaaa
    10321 tataaagata aaccgctata tccagcaaat aatactgtta gtcaggaaca agcaaataaa
    10381 tggcttacag aattaggtgg aacaatcggc aaggtatctc aaaaaaacgg gggatatgtt
    10441 gtcagtataa acatgacccc aatagacaat atgttaaaaa gcttagataa tctaggtgga
    10501 aatggcgagg ttgtgctaga taatgcaaaa tatcaggcat ggaatgccgg attctctgcc
    10561 gaagatgaaa caatgaaaaa taatcttcaa actttagttc aaaaatacag taatgccaat
    10621 agtatttttg ataatttagt aaaggttttg agtagtacaa taagctcatg tacagataca
    10681 gataaacttt ttctccattt ctgaggtgcg ccatgcataa tgtaaataat actcaagcgc
    10741 caacattctt atataaggca acttcaccat catcaacaga atacagcgag ttaaaaagca
    10801 aaatatccga tatccatagt tcgcaaactt ctctaaaaac accagcatca gtgtctgaaa
    10861 aagaaaactt tgcaacgtct tttaatcaga aatgtcttga ttttttattt tcttcctcag
    10921 ggaaagaaga tgtgttaaga agcatttatt ccaactcaat gaatgcgtat gccaaaagcg
    10981 agattctcga attttcaaat gttttgtact ccttagtaca tcaaaatggt cttaattttg
    11041 aaaacgaaaa gggacttcaa aaaattgtcg cacagtattc ggaactaatt ataaaagata
    11101 aattatccca agattctgcc tttggaccat ggtcggcaaa gaataagaaa ctccatcaat
    11161 tacgacaaaa cattgagcac agacttgcac tattagcaca acaacacaca tctggtgaag
    11221 ctttatcatt gggacaaaaa ctcctcaata ctgaagtatc atcatttatc aagaataata
    11281 ttcttgctga attaaagtta agtaatgaaa ctgtttcatc tctcaaacta gatgatttaa
    11341 ttgacgcaca ggcaaaactt gcctttgata gtttgcgcaa tcaacgtaaa aatactattg
    11401 atagtaaagg atttggtata ggtaaactgt caagagactt aaatacagta gccgtgtttc
    11461 ctgagctgtt gagaaaagtc cttaatgata ttttagaaga tataaaagat tcgcatccta
    11521 tccaagatgg cctccctaca cctcccgaag atatgccaga tggcggacca acccccggag
    11581 ccaatgagaa aacatcccaa cctgtaattc actatcatat aaataatgat aatagaactt
    11641 acgataatag agtttttgac aacagagtat atgacaatag ctatcacgag aacccagaaa
    11701 atgatgcaca gtctcctact tctcagacaa acgatctatt atcccgtaac ggaaactcat
    11761 tactaaatcc acaaagagca ctagttcaaa aagtaacttc cgttctacca cactctatat
    11821 cagatactgt ccagacattt gcaaataatt cagctttaga aaaggttttc aaccatactc
    11881 cagataattc ggatggaata ggttcagacc tgttaactac gagtagtcaa gaaagatctg
    11941 caaataactc tctttctcgg ggacacaggc ctctgaacat acagaactct tcaaccaccc
    12001 cccctctcca cccggaagga gtgacaagca gtaatgataa ctcatcagat acaactaaaa
    12061 gtagcgcttc tctttctcat agagtagctt cgcaaataaa taaattcaac tcaaacactg
    12121 attcaaaagt acttcagact gattttttat caagaaatgg agacacatat ttaacacggg
    12181 aaacgatatt tgaagcttca aaaaaagtaa caaactccct aagtaatctt atatctctca
    12241 ttggaactaa atcaggaaca caagaacgag agttacagga aaaatcaaag gacattacaa
    12301 aatccacaac agaacacaga ataaacaaca aattaaaagt tacagatgca aataaaataa
    12361 actacgtaac agaaaccaac gcagatacaa ttgataaaaa tcatgcgatc tatgaaaagg
    12421 caaaagaagt atctagcgcc ctcagcaagg tattgtcaca aattgacgat acctctgcag
    12481 aattacttac agatgatata tctgatttaa aaaataacaa tgatattaca gctgaaaaca
    12541 ataatatata taacgcagca aaagatgtaa ccacttccct atcaaaagta ttaaagaata
    12601 tcaataagga ttaaaatatg ataaaagaaa aaatattatc aatagtggcc ttttgctatg
    12661 gtatagcgta cagcaaacta tctgaagaaa ctaaatttat tgaagatctt tctgccgatt
    12721 ctctttctct gattgaaatg ctggatatga ttagttttga gttcaatcta cgtatagatg
    12781 aatctgcatt agaacacatt attactattg gagatcttat ttctgtagtc aaaaatagta
    12841 caaaatcaat atagtaattg ataagcattt tttcatctat ggagctctca catcagagct
    12901 ccacaagaat attattcttt tatccaataa agataaattg catcaatcca gctattaaaa
    12961 tagtaatttt taagactacc gttgactatc atcaacagtt acatacaatc ttgctcacac
    13021 tgcatttaac ttttgtcaat ataaatcaat atgaataaac agggtgtgat atggtggatt
    13081 tgtgcaacga cttgttaagt ataaaggaag gccaaaagaa agagtttaca ctccattctg
    13141 gtaataaagt ttcctttatc aaagccaaga ttcctcataa aaggatccaa gatttaacct
    13201 tcgtcaacca aaaaacgaat gtacgcgatc aagaatccct aacagaagaa tcattagccg
    13261 atatcataaa aactataaag ctacaacaat tcttccctgt aataggaagg gagattgatg
    13321 gtagaattga aattctggat ggcactcgta gaagagcatc tgcaatatat gcaggagcag
    13381 atcttgaagt tctatattca aaagaatata tatctactct tgatgccaga aaactagcaa
    13441 acgatataca aacagcaaaa gagcatagca tccgagaact tggtattggt cttaattttc
    13501 tgaaagtatc agggatgtcc tataaagaca tagccaaaaa agagaatctg tctcgcgcga
    13561 aagtcactcg tgcctttcag gcagcaagcg ttccacagga aataatatct ctatttccaa
    13621 tcgcgtcaga acttaacttt aatgactaca agatattatt caattattat aaaggacttg
    13681 aaaaggctaa tgaatctctt agttctacac taccaatatt aaaggaagaa ataaaagacc
    13741 ttgatacaaa tttgcccccg gacatatata aaaaagaaat tttaaacatc ataaagaaaa
    13801 gcaaaaacag aaagcaaaac ccttcgctaa aagttgactc gttatttatt tctaaagaca
    13861 aacggactta cataaaaaga aaagagaata aaacaaacag aactctaata tttacattat
    13921 ctaaaataaa caaaacagtt cagagagaaa tagatgaagc tattcgggat ataatttctc
    13981 gccatctatc gtcttcataa acatcgaaca ctgatgtaaa ctgcccaatt cagataataa
    14041 aaatcttcgt tgaacttgac tttcagccgg tggggtaatt ccgtcggcta tctgttattc
    14101 aatccccacg cactgtgttt ccttaaaatt acggtgaaaa ctcataccct gctgaccacg
    14161 agaaaattat ctggtgaaat tagtaatatt catatcattg aaaatgaatt gtgcaactaa
    14221 gggatgcggc atgatggtca aaattttatg tgaagatgag taactggccg aaaatattca
    14281 gcgtattttt aatcgtttca ataaaaacag cgaggattag cagtcaacgc ttttactact
    14341 caattttttt acatggtgat aaacaaaaaa tccaccatta acagacttcg ttcacagtat
    14401 caaaaaagct ttaagaatat attagcagat ttctcaaata taattactgt tttattctcc
    14461 taatcttccc ata
//
LOCUS       DADLZM010000104        13995 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLZM010000104 DADLZM010000000
VERSION     DADLZM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.105"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..423
                     /locus_tag="JNY73_002942"
     CDS             <1..423
                     /locus_tag="JNY73_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048884.1"
                     /translation="HDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLL
                     KGATAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSA
                     VHIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
     gene            595..702
                     /locus_tag="JNY73_002943"
     CDS             595..702
                     /locus_tag="JNY73_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048885.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(755..1509)
                     /locus_tag="JNY73_002944"
                     /pseudo
     CDS             complement(755..1509)
                     /locus_tag="JNY73_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
     gene            1795..2724
                     /gene="pfkB"
                     /locus_tag="JNY73_002945"
     CDS             1795..2724
                     /gene="pfkB"
                     /locus_tag="JNY73_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY8048886.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2825..3115
                     /gene="ghoS"
                     /locus_tag="JNY73_002946"
     CDS             2825..3115
                     /gene="ghoS"
                     /locus_tag="JNY73_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY8048887.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3221..4081
                     /locus_tag="JNY73_002947"
     CDS             3221..4081
                     /locus_tag="JNY73_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY8048888.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4122..4658)
                     /locus_tag="JNY73_002948"
     CDS             complement(4122..4658)
                     /locus_tag="JNY73_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048889.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4805..5472
                     /gene="hxpB"
                     /locus_tag="JNY73_002949"
                     /pseudo
     CDS             4805..5472
                     /gene="hxpB"
                     /locus_tag="JNY73_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            5635..6225
                     /locus_tag="JNY73_002950"
     CDS             5635..6225
                     /locus_tag="JNY73_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8048890.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6358..7748
                     /locus_tag="JNY73_002951"
                     /pseudo
     CDS             6358..7748
                     /locus_tag="JNY73_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(7752..8567)
                     /locus_tag="JNY73_002952"
     CDS             complement(7752..8567)
                     /locus_tag="JNY73_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048891.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINEDFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFA
                     AIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(8844..9107)
                     /gene="cedA"
                     /locus_tag="JNY73_002953"
     CDS             complement(8844..9107)
                     /gene="cedA"
                     /locus_tag="JNY73_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY8048892.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9290..11551
                     /gene="katE"
                     /locus_tag="JNY73_002954"
     CDS             9290..11551
                     /gene="katE"
                     /locus_tag="JNY73_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY8048893.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPRSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGHVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(11598..12356)
                     /gene="chbG"
                     /locus_tag="JNY73_002955"
     CDS             complement(11598..12356)
                     /gene="chbG"
                     /locus_tag="JNY73_002955"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY8048894.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVPDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12369..13721)
                     /locus_tag="JNY73_002956"
     CDS             complement(12369..13721)
                     /locus_tag="JNY73_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8048895.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RYTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(13826..>13995)
                     /locus_tag="JNY73_002957"
     CDS             complement(13826..>13995)
                     /locus_tag="JNY73_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8048896.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
BASE COUNT         3714 a         3453 c         3218 g         3610 t
ORIGIN      
        1 catgatggtg agccaggatt atacgccgca atggaaaata atcacccttt gtgtgtcaca
       61 cggttcctct ctaaaattaa tggcatcgcc tttaaataca agttgagcaa agctaacatc
      121 atggatttat taaaaggagc tacagcacag ggaacccctg cattatacat cgccatgagc
      181 aagggtaatg aagacgtcgt gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa
      241 cattctttta gtcaacatca gttatttaca ctattggccg ctaaaaatca tgacaacatg
      301 tcagctgttc atatagccat tcatcataag cattataaaa ctgtagaaac atattatgct
      361 gctataaatg tcatcagcca aagcctgagt tttagtgctg atgaaataaa gacgtattta
      421 taaccagcta tttacgatat atatcgatac ataattttat ttttttcata aaacgcagaa
      481 acagtgacca tgtgcacaac gccacccgac ggctgtaata acaaaaaatc ccttgatgcc
      541 tgtccctttt gttacactcc gttatcacgc acaagagata tgcaggacac tggtatgccg
      601 actaaacgct ttgataaaaa acactggaag atggtggtgg tgctactggc aatctgtggc
      661 gctatgttgt tgctacgttg ggcagcaatg atttggggct gagcagtgac aaacgaagac
      721 agccagaccg cataacacgg tccggcagat aattttacat gctgtagccc agcgataagg
      781 ttgtccgacg gtcggtatgt tccggtgcag actctggagg ttcagagttc catgtgacgt
      841 tgtaagccac cttcagtcca aaatgttcat taatagcaac atttaatgcg ctttcggagt
      901 tcagcgttgt gtcttccgcg ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag
      961 tcaactgcca tgcataagcc ccggaggcat agcccagcgg ctgggtttca ctggcattgt
     1021 cggtatattt gtcgtaacgc acacctggac cgaattcaaa gcggaaactg tgtaccgggc
     1081 cattcaaaaa ctgacgacca taacccgcgg tcaacacatc gcgctctcga tagccgttat
     1141 aacggtctgt cagccagctt gcctgaccaa ataaatagtc ataatcagtt aaattaaagc
     1201 ggctacgccc gccgcatatt tttctgaaga acgctcatca ttagaagaag tattactggc
     1261 gttcccccac agcgaccagg cagtggtttg tccataccag gtcatggtgg tgtcagccgt
     1321 aagtgaggag cttttcgtat tgcctgattg tgcaagatat cctgcgttca gattaccttc
     1381 gaaaggtttt ttagcgctgg caggatcgtc catgacagta aaaacggaat catcggcagc
     1441 tgcattcagt gacgcaaaca tgccccccgc caacataacg atggcaggaa ctgtcttcaa
     1501 aagcttcatt tatcaagagt ccgtacaaca aaaaagagac catcacggtc ccggaaactt
     1561 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     1621 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     1681 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     1741 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     1801 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     1861 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     1921 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     1981 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2041 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2101 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2161 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2221 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2281 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2341 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga gtcgcgaact cacccagcca
     2401 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     2461 gtttcccttg gtccacaagg agcgctgggt gttgatagtg aaaactgcat tcaggtggtg
     2521 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     2581 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     2641 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     2701 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     2761 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     2821 aactatggcc agtggcgatc ttgtccgtta tgtcataacc gtcatgttgc atgaggatac
     2881 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     2941 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3001 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3061 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3121 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3181 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3241 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3301 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3361 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3421 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     3481 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     3541 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     3601 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     3661 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     3721 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     3781 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     3841 cattttcgac cctgcctgct actggggtga ccgagagtgc gacctggcga tgttaccgct
     3901 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     3961 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4021 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4081 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4141 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4201 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4261 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4321 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4381 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4441 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     4501 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     4561 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     4621 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     4681 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     4741 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     4801 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     4861 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     4921 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     4981 tctttgtacg cccggcaacc gtggaatggg ccaagccgtc aggaagtagt agaacgggtt
     5041 attgcccgtg ccatttcact ggttgaagag acacgtccat tattaccagg cgtgcgcgaa
     5101 gctgttgcgt tatgcaaaga acaaggttta ttggtgggac tggcctccgc gtcaccacta
     5161 catatgctgg aaaaagtgtt gaccatgttt gacttacgcg acagtttcga tgccctcgcc
     5221 tcggttgaaa aactgcctta cagcaagccg catccgcaag tatatctcga ctgcgcagca
     5281 aaactgggcg ttgacccact gacctgcgtg gcgctggaag actcggtaaa tggcatgatc
     5341 gcttctaaag cagcctgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat
     5401 ccacgttttg tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac
     5461 cttctcggtt aatgaccagg ggcagtgatc acgctgcccc tggttcttta tctgaatttg
     5521 cattcaactg acggattaat cgtcaattta agagaaagag ttacaccgtc accacttccg
     5581 tgcactgtat aaaaatccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg
     5641 gcggaaggtc accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg
     5701 acgcccgtgc tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt
     5761 ttgttaccgg acatcgatca cccaaagtcg tttcttgggc agcgattaaa atggatatca
     5821 aaaccgatcg cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt
     5881 gcgctgctgg caacctttta ccttaaggtt ccggaaggct ggttcattcc ggctgatgcg
     5941 ctacaaggaa tggtgctggg ttatttgagc cacatacttg ccgatatgct gacacccgcc
     6001 ggtgttcccc tgctctggcc atgccgctgg cgtttccgct tgcctatcct ggttccccaa
     6061 aagggcaacc aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg
     6121 ccccattcat tacccgagaa cagcgctgtt cgttggtcat cgcaaatgat caataccttg
     6181 cagatccagt ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac
     6241 ctttctggaa taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt
     6301 gcgccaacac tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg
     6361 aactttccat taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag
     6421 acccgccata aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg
     6481 gtttttggcc ttgccctgca taccatttat ggttctgaca gccaggtact taaagattct
     6541 gtacagtggt ttaacatcgt tggtaacggc aatgttcaac tgctgcaaat gatcgttatg
     6601 ccgttagtct tcgcctctat tctgagcgcg gttgcccgtc tgcataacgc atctcagtta
     6661 ggcaaaatca gttttctgac catcggtacg cttttgttta ccacgctgat tgcggcgctg
     6721 gtcggtgtgc tggtcaccac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     6781 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatctga
     6841 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     6901 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     6961 tgaaactgct gaaggatgat gcgccgaaag gtgaacgcgt cttaaccgct atcgataccc
     7021 tacaaagctg agtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7081 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7141 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7201 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7261 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7321 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7381 gtcagaacgg ctgcgccggt ttgtatccgg caatgctggc ggtgatggtt gcgcctacgg
     7441 ttggcattaa cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta
     7501 gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc
     7561 ctgcgatggg cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg
     7621 acatgggccg tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc
     7681 agtggctgaa gcaaaccgat aaagccattc tggatagcga agacgacgcc gaactggcac
     7741 accgttaatt cttatgctgg caacggtccg ttttgtatag gggccgttgc tttacttttt
     7801 aaatgttcca tcgcggcgat gcatactgac tcatatttaa acagatcgtc agcgaaaaaa
     7861 atgtccaact tatcaataca ttcctggctc aatgaatgtg cctgtagtga gaagcttaaa
     7921 acctcatacc cgagacgttt tctaagtgaa ctatctggaa tactcataat atcttcaagc
     7981 acaccacttc ccattcccgg aagatttagt ggattaaaaa gaagattgat attaagcatt
     8041 gatattatcg aaggaaactt taggagactc tcattttttt gaatcgcagc aaaaatgctc
     8101 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8161 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8221 atcccaacaa aatcctcatt aatatctctt atccgaaact cactgccata aagattatta
     8281 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8341 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8401 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     8461 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     8521 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     8581 ttattatctt atttaaatct aagccattta caatataagt ttaagagcga cgccacagga
     8641 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     8701 caattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     8761 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     8821 caaaaccagg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     8881 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     8941 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9001 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9061 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9121 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9181 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9241 ttgaccaaaa aagcggccct tccagtaata aattaaggag acgagttcaa tgtcgcaaca
     9301 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9361 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9421 gccaggtgca caacctaccg cccctaggag cctgaaagcc cctgatacac gtaacgaaaa
     9481 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
     9541 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
     9601 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
     9661 acgtattgtt catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt
     9721 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
     9781 taggttctct accgttcagg gtggcgctgg ctctgccgat accgtgcgtg atatccgtgg
     9841 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
     9901 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
     9961 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10021 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10081 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10141 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10201 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10261 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10321 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10381 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10441 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    10501 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    10561 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    10621 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    10681 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    10741 ataccaggag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    10801 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    10861 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    10921 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    10981 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11041 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc acgtggtagc
    11101 gattttactt aatgatgaag tgagatcggc agaccttctt gccattctca aggccctgaa
    11161 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11221 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11281 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11341 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11401 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    11461 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    11521 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    11581 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    11641 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    11701 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    11761 ggatcacccc gatggcttgc gtcatccggc acttgcagga acagtgcctc gctaatctct
    11821 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattagccgg taaatcacca
    11881 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    11941 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12001 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12061 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12121 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12181 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12241 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12301 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12361 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12421 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    12481 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    12541 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    12601 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcttacaggt catttctacc
    12661 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    12721 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    12781 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
    12841 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    12901 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    12961 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13021 ccagatgctt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13081 ccatttacca gcacattctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13141 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13201 atattacaca cgccgataaa gcgtttaaat ccggtataac gataaacggc ttcagtaacc
    13261 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13321 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13381 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13441 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    13501 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    13561 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    13621 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    13681 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    13741 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    13801 tatgtgaatt gtcaggtata acgacttact gattaaattc cgtcaatttc ttacgatatc
    13861 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    13921 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgttttttgg
    13981 caaaattaat acgga
//
LOCUS       DADLZM010000105        13988 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLZM010000105 DADLZM010000000
VERSION     DADLZM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.106"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..454
                     /locus_tag="JNY73_002958"
                     /pseudo
     CDS             1..454
                     /locus_tag="JNY73_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            514..621
                     /locus_tag="JNY73_002959"
     CDS             514..621
                     /locus_tag="JNY73_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY8048897.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNY73_002960"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNY73_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY8048898.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNY73_002961"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNY73_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNY73_002962"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNY73_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNY73_002963"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNY73_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY8048899.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNY73_002964"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNY73_002964"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY8048900.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="JNY73_002965"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="JNY73_002965"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY8048901.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNY73_002966"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNY73_002966"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY8048902.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="JNY73_002967"
     CDS             complement(9943..10530)
                     /locus_tag="JNY73_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY8048903.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="JNY73_002968"
     CDS             10700..11278
                     /locus_tag="JNY73_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8048904.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="JNY73_002969"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="JNY73_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JNY73_002970"
     CDS             complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JNY73_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY8048905.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGMETDINASKTRQQ
                     LNDVVGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIRLTGALGGGYKR
                     "
     gene            13869..13952
                     /gene="yohP"
                     /locus_tag="JNY73_002971"
     CDS             13869..13952
                     /gene="yohP"
                     /locus_tag="JNY73_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY8048906.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3333 a         3905 c         3527 g         3223 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgcc cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attcgccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccaaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    13921 tggtgactgg cgtatttaag atgatatttt aaaattaatt aatgtcatca gatccgaaaa
    13981 taacgaga
//
LOCUS       DADLZM010000106        13890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLZM010000106 DADLZM010000000
VERSION     DADLZM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.107"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNY73_002972"
     CDS             complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNY73_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY8048907.1"
                     /translation="MSTSIRICSYLLLLLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSL"
     gene            complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNY73_002973"
     CDS             complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNY73_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY8048908.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKHQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNY73_002974"
     CDS             complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNY73_002974"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY8048909.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNY73_002975"
                     /pseudo
     CDS             complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNY73_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNY73_002976"
     CDS             complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNY73_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY8048910.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPSVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNY73_002977"
     CDS             complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNY73_002977"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY8048911.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6140..7279)
                     /locus_tag="JNY73_002978"
     CDS             complement(6140..7279)
                     /locus_tag="JNY73_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY8048912.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLIPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7938..9521
                     /locus_tag="JNY73_002979"
     CDS             7938..9521
                     /locus_tag="JNY73_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY8048913.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNY73_002980"
     CDS             complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNY73_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY8048914.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNY73_002981"
     CDS             complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNY73_002981"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY8048915.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNY73_002982"
     CDS             complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNY73_002982"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY8048916.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13480..>13890
                     /locus_tag="JNY73_002983"
     CDS             13480..>13890
                     /locus_tag="JNY73_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048917.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPL"
BASE COUNT         3226 a         3666 c         3509 g         3489 t
ORIGIN      
        1 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
       61 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
      121 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
      181 taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc actccagcaa
      241 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
      301 aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
      361 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
      421 gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
      481 cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
      541 caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt gtggcgaaat
      601 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
      661 agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt taaacgccgt
      721 gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc gttctaaaaa
      781 cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg
      841 ggcgattttg acgttaacca gcaggtagat cagtagcagc agcaggtagc tacagattct
      901 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cgactgcgtt
      961 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
     1021 gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt tgcagcactt
     1081 cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
     1141 aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc tgattgagcg
     1201 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
     1261 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
     1321 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
     1381 tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct tcggttgcca
     1441 tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt
     1501 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcgcggaata
     1561 actggtgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat
     1621 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
     1681 agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca tccggtttat
     1741 ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag tagctgtgca
     1801 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
     1861 tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg
     1921 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgaca ctctctttgc
     1981 cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     2041 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     2101 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
     2161 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     2221 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     2281 atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
     2341 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
     2401 cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
     2461 cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
     2521 agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt tggcgcgcag
     2581 atcttccagc atctttagcg ccccgaatac catcagccag acgtttgccg ttacgcaccg
     2641 acagcagcgt caacaaggtg cgccaggtca tacgtttatt gcggatctgg tagggggtaa
     2701 acagctgata ttttttactg gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga
     2761 agtatccgga gaattttttc ggtgacgagg tgatctgcat ttcgccgtga ttgatatgca
     2821 ggatctgcgt cgcctcttcc actttccacg gttcgccgta ctccaccacc atccgcagga
     2881 atatgtcgta atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc
     2941 atgcccaggt aaagacctga ttgccaatga tattacgttt atagaacagg cggcgtgagt
     3001 acggcgattt gggatacagt ggtaagctcg ccggttggga ataaacttct ccctggcaaa
     3061 cgtagtcgtt agcgtacaaa aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga
     3121 cgctcaggcg gttgggcgtc cattcatcat catcatcgat cccggtgatg tattcccctt
     3181 gcgccagcat aatcgcctgg ttacgcaccg cgcacgcccc gctgttaatg tcgttgtgga
     3241 tgtaagtgat acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg
     3301 tggagcaatc atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt
     3361 ttatcgcccg aatcgccagt tgttggcggt tccaggttgg catatagatt gagatcagcg
     3421 gattgtcttt cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg
     3481 tgagcgcctt atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga
     3541 cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga
     3601 gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc
     3661 cacttctttc aatgtgttga ccgcataacg cgccaccatt aacgtggttc cgacatgacg
     3721 accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc
     3781 atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatta acagttcaga
     3841 aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt
     3901 agcagcggta gtaatatcgc cctgaccaat cagaatctcc gacagcccat tgacattatt
     3961 ggtgcccaac agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacgcg
     4021 tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt taccgattga
     4081 cgggctaacc ccggtcatca tcaacacatt gttctgcgcc tgcatcatcg cgaagtgcag
     4141 actggtacgc agactacgga tggcttcaat cgccagatcg gttggattcc ccactgccag
     4201 tagctggctc tgtttatagc gtttaacccc tttgatggtt ttgacgctat cgcgcgcttt
     4261 ctgccactcc gacagcggga tgctggcata gacgctgatt ccgtgttctt ccagcacttg
     4321 cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat
     4381 caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta gcacgctagg
     4441 ctgggtgatt gccgggtcaa taatgcgcac atcgccgacg gtgctggctt cggtgatttt
     4501 cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg
     4561 ggtcagacgg acaatttcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt
     4621 ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac
     4681 tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc
     4741 gatgttcacc atcgaatcga gcaccgcttt cgcttcaagc ggcagatcaa cagaatcttt
     4801 atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg
     4861 taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt
     4921 ttgttcctga tagttacgag cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc
     4981 accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct
     5041 gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc
     5101 cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc
     5161 cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt tgtcatccag
     5221 cacattaagc gtaaacacct gatccgccat ctctttcggg cggttaaagg tagtcacttt
     5281 caccgtctcg ttctgacgcc ccatcaggcg atcccagccc gccccgaaaa tcgggaacgt
     5341 gtttttgctc accgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg
     5401 cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgagcc
     5461 gatatcctgc accagcgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact
     5521 ataaatcggc gtggcgaaga aagtgtaaac cacggcacag agggcaaata cgacggtgat
     5581 gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc
     5641 atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc
     5701 tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct
     5761 gcaaacgttt cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga
     5821 ccaaacagca tcactttgcc gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg
     5881 cgcttatcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt
     5941 gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca
     6001 gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa
     6061 cgttgcagta agcgttccgc catcggggaa cggcaaatat tgcctacaca gacaactaag
     6121 atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac
     6181 ggacaccgct aatagttggc agcaactgat tgatcagacg gttccagcgg gaaaccgggg
     6241 cggtggtgac atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg
     6301 acgtcgcatc ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgccct
     6361 cgcctttcag cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac
     6421 cttcggcatt gcccagagct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc
     6481 tctgtttctt cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga
     6541 gaatatcgcc gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct
     6601 gtaaagaaat gcgttcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg
     6661 tgtcggtcag gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggt acgttggtga
     6721 tcgcctgttg accggattta ttcacctgac ctgagatata ggccttttgt gagcggaagg
     6781 cggcgatatt aacgtccacc tgcgggtcag cgatgtatgt cgctaagcgc ccggtaatat
     6841 cactgcggat ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa
     6901 acatagtgcc gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccag
     6961 ctggcgtggt gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga
     7021 cgcgatactg gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg
     7081 ggcgtgggcg taattggtca atcaggcgcg ggatcagcgg ataaacattc accattttgt
     7141 cgagatcgaa atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc
     7201 tgcccggaag tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt
     7261 tcattttgga tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact
     7321 attaatagca atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg
     7381 gcatcagtcc taaaaaaatt gatttcatca gcaggctatt gacagaataa ttcagactgg
     7441 tctttcaggc atccagacac gctaccgccc ctggcttttt agctaccaat acactgattg
     7501 agcttaattt ttcaaaccct ctctgcatac aatcgttgat gagaaagggt tattgcggaa
     7561 attaacttcc gaatataagg tgacattatg gtaattgaat attggctcgc caataatgca
     7621 ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt
     7681 tttaagaatt atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa
     7741 tatttaatat tgcgtctcca catgcgatat ttcttaaata atgttttatt attaccgctt
     7801 ttaattcagg gataatgtga ggttattatc ctcaaataat atgagataaa tagtgctgca
     7861 acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa
     7921 taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca
     7981 cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg
     8041 ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg ctgctggcga
     8101 tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg actcaaccgc
     8161 tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt
     8221 tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca
     8281 ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac
     8341 tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac
     8401 tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt
     8461 taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc ttcctgttga
     8521 tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt
     8581 acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct atctttaacc
     8641 ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa gcggtaatgc
     8701 gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg
     8761 tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac
     8821 ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata
     8881 tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc
     8941 ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg
     9001 acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc
     9061 cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc cgtaatgccc
     9121 gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa
     9181 gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag atcgacgccc
     9241 gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg
     9301 aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg
     9361 ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg
     9421 gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta
     9481 taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg ccattttacc
     9541 caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt aaacggacat
     9601 cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag gctggggcag
     9661 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgtacccg
     9721 atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg
     9781 ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat
     9841 agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat
     9901 agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggacagg
     9961 tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc
    10021 tttattccgc tcggcccagt cattcaagcg acgtttcgct tcgtcctgta gatgtttgcg
    10081 cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag
    10141 cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac
    10201 gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc
    10261 tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt
    10321 caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt tttcagcggc
    10381 cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc
    10441 ttccatacgt gtgtcggtca tctcaacatg cgcctgccct tgccagttgt ggcggaatgc
    10501 gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga
    10561 aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca gccgtggctg
    10621 gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctttcc
    10681 accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg
    10741 cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat
    10801 atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac
    10861 cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc
    10921 ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg
    10981 ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc
    11041 ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact gggactggaa
    11101 actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat
    11161 ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag
    11221 ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg
    11281 atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc
    11341 ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat caaacgacca
    11401 tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc
    11461 actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg
    11521 cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc
    11581 ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat
    11641 actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct
    11701 acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac
    11761 taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg
    11821 tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc
    11881 ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga
    11941 aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt
    12001 accggagttg tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc
    12061 ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac
    12121 cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg
    12181 aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc
    12241 ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa
    12301 ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg
    12361 cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca
    12421 cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg
    12481 cataccagct tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta
    12541 ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt
    12601 ccgcgtattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat
    12661 attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc
    12721 gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca
    12781 aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaacct
    12841 tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg
    12901 agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc gcgctgggat
    12961 ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc
    13021 agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa
    13081 gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc
    13141 actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg
    13201 ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat
    13261 agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt
    13321 gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac
    13381 gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga
    13441 aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca atcacagcat
    13501 gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt
    13561 atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg
    13621 ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga
    13681 attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc
    13741 tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg
    13801 ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg
    13861 gcattcggca gcgccattgt tccgcctctg
//
LOCUS       DADLZM010000107        13829 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLZM010000107 DADLZM010000000
VERSION     DADLZM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.108"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            166..1419
                     /gene="kch"
                     /locus_tag="JNY73_002984"
     CDS             166..1419
                     /gene="kch"
                     /locus_tag="JNY73_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY8048918.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1474..1647)
                     /locus_tag="JNY73_002985"
     CDS             complement(1474..1647)
                     /locus_tag="JNY73_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY8048919.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1790..3250
                     /gene="cls"
                     /locus_tag="JNY73_002986"
     CDS             1790..3250
                     /gene="cls"
                     /locus_tag="JNY73_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY8048920.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3285..3614
                     /locus_tag="JNY73_002987"
     CDS             3285..3614
                     /locus_tag="JNY73_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY8048921.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="JNY73_002988"
     CDS             complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="JNY73_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY8048922.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="JNY73_002989"
     CDS             complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="JNY73_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY8048923.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLRACFKPVE
                     ELL"
     gene            complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="JNY73_002990"
     CDS             complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="JNY73_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY8048924.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="JNY73_002991"
     CDS             complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="JNY73_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY8048925.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="JNY73_002992"
     CDS             complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="JNY73_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY8048926.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="JNY73_002993"
     CDS             complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="JNY73_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY8048927.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11115..13790
                     /gene="adhE"
                     /locus_tag="JNY73_002994"
                     /gene_synonym="adhC"
     CDS             11115..13790
                     /gene="adhE"
                     /locus_tag="JNY73_002994"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY8048928.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3627 a         3426 c         3223 g         3553 t
ORIGIN      
        1 gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt tgattactgc
       61 ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt tcagaaaatg
      121 tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag tcactgggct
      181 acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat tctcgcgctg
      241 gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc ggtcaatttg
      301 cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct ggcaaacgcc
      361 ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact gttccgggca
      421 aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta caccctgcat
      481 ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt gtttttgctg
      541 atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt tgcatttatt
      601 agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag cgaaggtttt
      661 aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac catgtcaacc
      721 gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac tatttcggtc
      781 attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg tccgcttatc
      841 cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg taaagatcat
      901 tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact gaatcaacgc
      961 ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca acttgagcaa
     1021 cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc agtattaaag
     1081 aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga tgcagataac
     1141 gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc
     1201 agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat tctctcaccg
     1261 caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat taataatgat
     1321 atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa cgatgaacaa
     1381 gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac ggaagagtaa
     1441 acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg caaacaataa
     1501 cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg
     1561 cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg
     1621 ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat
     1681 tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat
     1741 ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt
     1801 ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac
     1861 tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat
     1921 ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg
     1981 caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga
     2041 ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt
     2101 caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat
     2161 gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa
     2221 tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc
     2281 attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact ccgccgggag
     2341 tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt
     2401 cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca
     2461 tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga
     2521 tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat
     2581 ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac
     2641 cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg aacaggccag
     2701 tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca
     2761 ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca cgccctactt
     2821 tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt
     2881 cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt
     2941 ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca
     3001 taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta accttgatat
     3061 gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag gttttggtgc
     3121 agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt
     3181 atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagccc
     3241 gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa
     3301 acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt ctcgaactgg
     3361 ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg
     3421 gcggcgcgga gttattcgat ccggtggaag actggcagga acatgttgat ttcgatttga
     3481 acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat ggtgaaatca
     3541 acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca
     3601 tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg tcagccctta
     3661 ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccctccag
     3721 aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca
     3781 accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt
     3841 cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg
     3901 tagtggattg tggtagacct catcataggt ccccagttct actgcatggc cgagatacat
     3961 caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa taaaaattaa
     4021 tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg cctgaattga
     4081 cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa gaataagagc
     4141 acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat
     4201 caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg
     4261 gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat
     4321 acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac tgcgaacggc
     4381 acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc
     4441 ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc atcccgattc
     4501 ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt
     4561 gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt
     4621 aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc
     4681 tccaccggtt taaagcaagc acgcagacgg ccaagcgtaa actcttccag cggcggtgcg
     4741 ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat
     4801 cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga
     4861 cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg
     4921 cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc
     4981 cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa ttcccgcttc
     5041 agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc tgtagtgggt
     5101 tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat gactcgctga
     5161 cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc ttccggcatt
     5221 tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta
     5281 tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt caacgaagtc
     5341 attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcacgctct
     5401 ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg tccgttggca
     5461 gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca
     5521 atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg
     5581 ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt
     5641 tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact ccttaacgat
     5701 ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagtgtca
     5761 ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc gagttcgcgc
     5821 catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac cccaggaagc
     5881 taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag accaccacca
     5941 caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat acaccgccaa
     6001 cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac
     6061 gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg atgttttggc
     6121 cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca
     6181 gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa
     6241 gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc
     6301 cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac
     6361 cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact
     6421 gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg
     6481 caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt
     6541 cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca
     6601 tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac
     6661 aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta acactaagga
     6721 atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat
     6781 ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca tataggagag
     6841 cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc
     6901 tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag agccacgggt
     6961 aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa
     7021 tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat
     7081 catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc
     7141 catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac
     7201 acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa cgggaaaact
     7261 ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcgcc
     7321 atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata
     7381 tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg taaaagggct
     7441 tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat
     7501 cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct
     7561 tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc
     7621 attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg
     7681 tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa
     7741 tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct
     7801 cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg
     7861 cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt
     7921 ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg tcgaggaacg
     7981 ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag
     8041 aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg ttgatggtca
     8101 acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt
     8161 tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca gtatacggtg
     8221 gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc
     8281 ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt
     8341 tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct
     8401 cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg tcgatttcac
     8461 cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt
     8521 taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt
     8581 ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc
     8641 atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa
     8701 gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa
     8761 tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg
     8821 cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac
     8881 gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat
     8941 ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc
     9001 caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga
     9061 tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat
     9121 tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca
     9181 gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg
     9241 tgatgttggt cattgttttt tggactccct cattataatt actggtatta accagcatgt
     9301 gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat
     9361 cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat caccttatgt
     9421 cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg
     9481 ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc
     9541 ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt attgaccgta
     9601 tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag
     9661 attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg cacaataatt
     9721 taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt actaatatca
     9781 tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag
     9841 gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta
     9901 ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga
     9961 gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt
    10021 aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt
    10081 aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa
    10141 tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat
    10201 gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa
    10261 cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg atttttcttg
    10321 cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac
    10381 caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt gtagaatcgt
    10441 gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt
    10501 agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat
    10561 ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc
    10621 aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat
    10681 aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac
    10741 ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac
    10801 caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat gctgaaaggt
    10861 gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt gagtaatctt
    10921 gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta aactctttta
    10981 gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcattggtt aagcaaatca
    11041 tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa gtttaacatt
    11101 atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc gtagagcgtg
    11161 taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac aaaatcttcc
    11221 gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg gccgttgccg
    11281 aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct tctgaatata
    11341 tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac gacacttttg
    11401 gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg accactaacc
    11461 cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac gccattatct
    11521 tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc gttctgcagg
    11581 ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg
    11641 aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg actggtggtc
    11701 cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta ggcgcgggta
    11761 acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca tctgtactga
    11821 tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt gttgttgttg
    11881 actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat ctgttgcagg
    11941 gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg aacgcggcta
    12001 tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta ccagaaaaca
    12061 ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa
    12121 aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag
    12181 cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac actgaccagg
    12241 ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct cgtatcctga
    12301 ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt
    12361 ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac gttggtccga
    12421 aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg tggcacaaac
    12481 ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat gaagtgatta
    12541 ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac aatggttatg
    12601 ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc ttcttcgaag
    12661 tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca aactccttca
    12721 aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg aagatcatgt
    12781 gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc tttatggata
    12841 tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg atcgctgtca
    12901 ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact gacgacgcta
    12961 ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg attgtcgacg
    13021 ccaacctggt tatggacatg ccgaagtccc tttgtgcttt cggtggtctg gacgcagtaa
    13081 ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat ggtcaggctc
    13141 tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa
    13201 atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc gcatttgcga
    13261 acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag ttccatattc
    13321 cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat gcgaacgaca
    13381 acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc cgtcgttatg
    13441 ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct gctaagatcg
    13501 agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg aaatctatcc
    13561 gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg tctgaagatg
    13621 cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac
    13681 agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa
    13741 aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa tcagtagcgc
    13801 tgtctggcaa tataaacggc cccttctgg
//
LOCUS       DADLZM010000108        13714 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLZM010000108 DADLZM010000000
VERSION     DADLZM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.109"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..161)
                     /locus_tag="JNY73_002995"
     CDS             complement(<1..161)
                     /locus_tag="JNY73_002995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048929.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCA"
     gene            180..251
                     /locus_tag="JNY73_002996"
                     /pseudo
     CDS             180..251
                     /locus_tag="JNY73_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858230.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            252..1748
                     /locus_tag="JNY73_002997"
     CDS             252..1748
                     /locus_tag="JNY73_002997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAY8048930.1"
                     /translation="MCAQQSYDQHLAASTENWRQWWQKRRITVNGGDAHDQQALDYAL
                     YHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRW
                     HNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLV
                     ADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYT
                     EHVNNNAFTSYMVYYNVQQALSIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQ
                     DDSFMTKPAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSSA
                     SCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDD
                     GIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDA
                     QRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE"
     gene            1745..2404
                     /gene="pgmB"
                     /locus_tag="JNY73_002998"
     CDS             1745..2404
                     /gene="pgmB"
                     /locus_tag="JNY73_002998"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY8048931.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            2418..3500
                     /locus_tag="JNY73_002999"
     CDS             2418..3500
                     /locus_tag="JNY73_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8048932.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            3545..4450
                     /gene="ompG"
                     /locus_tag="JNY73_003000"
     CDS             3545..4450
                     /gene="ompG"
                     /locus_tag="JNY73_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY8048933.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(4561..5559)
                     /locus_tag="JNY73_003001"
     CDS             complement(4561..5559)
                     /locus_tag="JNY73_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY8048934.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            5714..7111
                     /locus_tag="JNY73_003002"
     CDS             5714..7111
                     /locus_tag="JNY73_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY8048935.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7108..8169
                     /locus_tag="JNY73_003003"
     CDS             7108..8169
                     /locus_tag="JNY73_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY8048936.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            8317..9858
                     /gene="tyrR"
                     /locus_tag="JNY73_003004"
     CDS             8317..9858
                     /gene="tyrR"
                     /locus_tag="JNY73_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY8048937.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(9902..10408)
                     /gene="tpx"
                     /locus_tag="JNY73_003005"
     CDS             complement(9902..10408)
                     /gene="tpx"
                     /locus_tag="JNY73_003005"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY8048938.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            10527..11486
                     /gene="ycjG"
                     /locus_tag="JNY73_003006"
     CDS             10527..11486
                     /gene="ycjG"
                     /locus_tag="JNY73_003006"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY8048939.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(11461..12189)
                     /gene="mpaA"
                     /locus_tag="JNY73_003007"
     CDS             complement(11461..12189)
                     /gene="mpaA"
                     /locus_tag="JNY73_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY8048940.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(12480..13118)
                     /locus_tag="JNY73_003008"
                     /pseudo
     CDS             complement(12480..13118)
                     /locus_tag="JNY73_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(13136..13666)
                     /locus_tag="JNY73_003009"
     CDS             complement(13136..13666)
                     /locus_tag="JNY73_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8048941.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
BASE COUNT         3334 a         3276 c         3705 g         3399 t
ORIGIN      
        1 tctgcgcagc attcaacggc atcgtcaaaa ctatcaaagc gcagaacttc tgcgtcttct
       61 tcgtcaaaaa aatcatcttc gtgaagcttc acgacatagc ggggaagttt gcttctttga
      121 gaggcgggtt tacgtttacg gggtttagct gaacgggcca tataaccacc tgaaagacaa
      181 tgacattgcc tgtttttata acggtaattg cagaccatga caagccgcag ccgtcaggct
      241 gcctactcga aatgtgcgcg caacagagtt acgaccaaca tcttgcagca tcaacagaaa
      301 actggcgtca atggtggcag aaacgtcgta tcacggtaaa tggcggcgat gcgcacgatc
      361 agcaagcgtt agattatgcg ctttatcatc tgcgcatcat gacgccggct cacggcgagc
      421 gcagcagtat tgcggcaaaa ggcttaaccg gcgaaggcta caaaggccac gttttctggg
      481 atacagaagt atttttgctg ccgttccatc tgtttagcga tccgacggtt gcccgaagtt
      541 tactgcgtta tcgctggcac aacttgccag gcgcgcagga gaaagcgcga cgcaacggct
      601 ggcagggcgc gctatttccg tgggaaagcg cgcgcagcgg cgaagaagag acgccggaat
      661 ttgccgccat taacattcgc accgggctgc ggcaaaaagt ggcctcggcg caggcggaac
      721 atcatctggt ggccgatatc gcctgggcgg ttattcaata ctggcagacc acgggggatg
      781 aaagtttcat tgcgcatgaa ggcatggcgc tacttctgga aactgcaaag ttctggatta
      841 gccgcgcggt gagggttaac gaccgtctgg aaattcatga tgttattggg ccagacgaat
      901 ataccgaaca tgtcaataat aacgccttca ccagctatat ggtgtattac aacgtccagc
      961 aggcgctgag tattgcccgc cagtttggct gtagtgacga cgcgtttatt catcgtgccg
     1021 aaatgttcct taaagaactg cggctgccag aaattcagcc cgacggtgtt ttgccgcagg
     1081 atgattcgtt tatgactaag ccggcgatta atctggcgaa atacaaagcg gcggcgggga
     1141 agcaaaccat tctgctggat tattcacgcg cagaagtgaa cgagatgcag atcctcaaac
     1201 aagctgatgt ggtgatgctc aattacatgc tgccagagca gttctcatcg gcatcgtgtc
     1261 ttgccaatct gcaattttat gaaccgcgca ctattcacga ctcgtcatta agtaaagcaa
     1321 tccacggcat tgttgctgca cgctgtggcc tgctgaccca aagttatcag ttctggcgcg
     1381 aggggactga aatcgatctt ggtgctgatc cgcatagttg tgatgatggt atccatgctg
     1441 ccgcaactgg cgctatctgg ctgggggcga ttcagggttt tgccggggtg agcgtgcgtg
     1501 acggtgaatt gcatctaaat ccggcgttac ctgagcagtg gcaacagttg tctttccctc
     1561 tgttctggca gggctgcgaa ttacaggtca ctctcgacgc gcagcgtatt gcgattcgaa
     1621 cttctgcgcc cgtttcactg cgtttgaacg gtcagcttat atccgtggct gaagaatctg
     1681 ttttctgttt gggtgatttt attttgccct tcaatgggac cgctaccacg catcaggagg
     1741 atgaatgaaa ctgcaagggg taattttcga tctggatggt gtaatcaccg ataccgcgca
     1801 tctgcatttc caggcgtggc agcagattgc cgctgaaatt ggcatcagta ttgatgcgca
     1861 gtttaacgaa tctctaaaag ggatcagccg cgatgagtct ctgcggcgca ttctgcaaca
     1921 cgggggcaaa gagggcgact ttaacccgca ggagcgggcg caactggcgt atcgcaaaaa
     1981 tctgctttac gtccactcac tgcgcgagct gacggttaat gctgtgctgc ccggtattcg
     2041 caatttgctg gcagagctcc gtgcacagca gattccggta gggctggctt ccgtctccct
     2101 gaatgcgccg acgattttag cggcgctgga gctgcgcgag tttttcacct tctgcgcgga
     2161 tgcttcccaa cttaaaaact cgaaaccgga tccggaaatc tttctcgctg cctgtgccgg
     2221 gctgggcgtg ccgccgcagg catgtatcgg cattgaagat gcgcaggcgg gcattgacgc
     2281 cattaacgcc agcggtatgc gctcggtggg gatcggtgcg ggcttaaccg gggcgcaatt
     2341 actgttgccc tcaacggact ccctgacctg gtcgcggtta tcggccttct ggcaaaacgt
     2401 atagcaaagg aatcaacatg gctcagcttt cgttacaaca tattcaaaaa atctacgata
     2461 accaggtgca tgtggtgaag gacttcaacc tggaaattgc cgataaagag ttcatcgtat
     2521 ttgtcggccc gtcgggctgc ggtaaatcga ccaccctgcg catgattgcc gggcttgagg
     2581 agatcagcgg cggcgatctg ttgatcgacg gcaaacgaat gaatgacgtt ccagccaaag
     2641 cacgcaatat cgcgatggtg ttccagaact acgcgttgta tccgcatatg acggtttacg
     2701 acaacatggc gtttggcctg aagatgcaaa aaatcgccaa agaggtgatt gatgagcggg
     2761 tgaactgggc ggcgcaaatt ctcggcctgc gtgagtacct gaaacgcaag ccgggcgcgc
     2821 tttccggcgg gcaacgccag cgtgtggcgc tcgggcgggc gattgtgcgc gaagcgggcg
     2881 tgtttttaat ggatgaaccg ctctctaacc ttgatgccaa gctgcgcgta caaatgcgtg
     2941 cagagatcag caagctgcat cagaaactga acaccaccat gatctacgtg acccacgatc
     3001 agaccgaagc gatgaccatg gcgacgcgga ttgtgattat gaaagacggg attgttcagc
     3061 aagtaggtgc gccgaaaacc gtttataacc aacccgcgaa tatgtttgtt gccggattta
     3121 ttggatcacc agcaatgaat tttattcgcg gcacgatcga tggcgataaa ttcgttacgg
     3181 aaacgcttaa attaaccatt ctcgaagaga aattagcggt tctgaaaaca caggaaagtt
     3241 tgcataagcc catcgtgatg ggaatacgcc cggaagatat tcatccggac gcgcaagagg
     3301 aaaataacat ttccgccaaa attagcgtgg cagaattaac cggtgcggaa tttatgctct
     3361 acaccacggt tggggggcac gagttagtgg tccgtgctgg tgcgttaaat gattatcatg
     3421 caggagaaaa tatcactatt cattttgata tgacgaaatg tcatttcttt gatgcagaaa
     3481 cggaaatagc aattcgctaa atacaggggg aaggcattcc cctaggataa tacaaggaac
     3541 aataatgaaa aagttattac cctgtaccgc actggtgatg tgtgcgggaa tggcctgcgc
     3601 acaggccgag gaaaagaacg actggcactt taatatcggt gcgatgtacg aaatagaaaa
     3661 cgtcgagggt tatggcgaag atatggatgg gttggcggag ccttcagtct attttaatgc
     3721 cgccaacggg ccgtggagaa ttgctctggc ctattatcag gaagggccgg tagattatag
     3781 cgcgggtaaa cgtggaacgt ggtttgatcg cccggagctg gaggtgcatt atcagttcct
     3841 cgaaagcgat gatttcagtt tcggcctgac cggcggtttc cgtaattatg gttatcacta
     3901 cgttgatgaa ccgggaaaag acacggcgaa tatgcagcgc tggaaaatcg cgccagactg
     3961 ggatgtgaaa ctgactgatg atttacgttt caacggttgg ttgtcgatgt ataaatttgc
     4021 caacgatttg aacacgaccg gttacgctga tacccgtgtc gaaacggaaa caggtctgca
     4081 atataccttc aacgaaacgg ttgctttgcg agtgaactat tatctcgagc gcggcttcaa
     4141 tatggacgac agccgcaata acggtgagtt ttccacacaa gaaattcgcg cctatttgcc
     4201 gctgacgctc ggcaaccact cggtgacgcc gtatacgcgc attgggctgg atcgctggag
     4261 taactgggac tggcaggatg atattgaacg tgaaggccat gattttaacc gtgtgggttt
     4321 attttacggt tatgatttcc agaacggact ttccgtttcg ctggaatacg cgtttgagtg
     4381 gcaggatcac gacgaaggcg acagcgataa attccattat gcaggtgtcg gcgtaaatta
     4441 ctcgttctga taatgggcta aattgccgga tgcggcgcga gtactttatc cgatctataa
     4501 atgtaggccg gataagatgc gccagcatcg catccggcat tcaggcaagg tagctggtat
     4561 ttatttcagc gtcatatgcg tggcaacggt aatattctgt ggtgacggtt ttcccgaaat
     4621 taagcggaat aataactcgc agctttgttg acctaactcc tgcgtcggaa catcgatgcc
     4681 gcctggtgca ggcgttaaaa taaacgacag cgtttcatta ctataaccca ccaccgctaa
     4741 ctgctgcgga atagcaatat ttttctctgc cgccgcacga taaatgctca ttaatttcag
     4801 gctgtcagtg gcaaacactg cctcaggcaa cggtgactga cttaataatt gccgtgctgc
     4861 ttgcagtgct gtttcatggg tataaccgcc gtcaacaatc catttatcac gcactgcaat
     4921 attatgcgcg gacagacttt tcttatagcc attaacgcga tcaaccgaaa catggacatc
     4981 aagcggagca tgcaggcagg caatattttg atgcccactt tcaattaatg catcggtcaa
     5041 cgcaatgctg tcaccaaaat tatcggtatc gacagaataa acatgggcat attgaccttc
     5101 aactttgcca attaccacca ccggaatatc gtatttatcg agttgggcaa aaaatgactc
     5161 atccgctggc gaactgagca taataatgcc tttaatcatt ttctgtttaa ttttgctttc
     5221 gcatttttgc agatcttctg ccggattgtg cgacgtttgt aatatcacgt cgaaaccttc
     5281 ttcttccgct ttggcggtga tggcatgcaa aacttcagag aaaaacggat tacccgccgt
     5341 agttttggtc gaacgggtag aaatcaccat aatggcatca aaacccgaag aggtcagcgc
     5401 gcgggccagc ttgtttggct gatactgtaa ttcttcaatg gcccgtaaca ctttttcgcg
     5461 cgcttccggg gagatattag tttgcttatt cagcacgcgt gatacggtgg attttgatac
     5521 gcctgcaacc ctggcaatat cataaatagt aggggacata ggtcaggaac tccgtccgaa
     5581 cgtgaatgct gcacatctta tggagttcgc ccgtcgatga caacacgggc agttgataat
     5641 caatggcttg gcccccacat tcatatcctt acgaatgatt tttttctggt cttcagagca
     5701 gcacaggaca gcaatgaagc gacttaaaaa tgaactcaat gcgctggtga atcggggtgt
     5761 cgacagacat ctgcgcctcg ctgtaaccgg acttagccgc agcggcaaaa cagcgtttat
     5821 caccgcgatg gttaatcagt tgctcaatat tcatgccgga gcacgtttgc cgctgttaag
     5881 cgcggtgcgt gaagagcgct tgctgggcgt aaaacgcatt ccccagcgtg actttggcat
     5941 tccgcgcttc acctatgatg aaggactggc gcagttatat ggtgatcctc ccgcctggcc
     6001 gacgcctacg cgcggcgtca gtgaaattcg cctggcgtta cgttttaaat cgaacgattc
     6061 gctgctgcgc cactttaagg atacctccac gctgtatctg gaaattgtgg attatcccgg
     6121 cgaatggttg ctcgacctgc cgatgctggc gcaggattac ttaagctggt cgcgccagat
     6181 gacgggctta ctcaatggtc agcgcggcga atggtcggcg aaatggcgaa tgatgtgcga
     6241 aggacttgac ccgctagcgc ctgccgacga aaaccggctg gcagacattg ccgccgtgtg
     6301 gaccgattat ctccaccact gtaaacagca ggggctgcac tttattcagc ctgggcgctt
     6361 tgtcttgccg ggagatatgg caggtgcgcc cgcgctgcaa ttcttcccgt ggccggatgt
     6421 cgatgcctgg ggcgagtcca aactggcgca ggccgataag cacaccaatg ccggaatgct
     6481 gcgcgagcgg tttaattatt actgcgagaa agtggtgaag gggttctata agaatcattt
     6541 tctgcgcttt gaccgccaga ttgtgctggt ggattgcctg caacctctca acagtgggcc
     6601 acaggcattt aatgatatgc gtctggcact gacgcagctg atgcaaagtt tccactacgg
     6661 gcagcgcacg ctgtttcgcc gcttattctc gccggttatc gacaaactgc tgtttgccgc
     6721 gacgaaagcg gaccatgtaa cggtcgatca gcacgccaat atggtttcat tactgcaaca
     6781 actgattcag gatgcctggc aaaatgcggc gtttgaaagg attagtatgg attgcctggg
     6841 gctggcctcg gtgcagacga ccaccagcgg cattattgac gtcaacggcg agaaaattcc
     6901 ggcattgcgc ggtaaccggc tcagcgatgg tgcgcctctc accgtttacc ccggtgaagt
     6961 tcctgcgcgt ttgcccggtc aggcgttctg ggacaagcaa gggttccagt ttgaagcgtt
     7021 tcgcccgcag gtgatggatg tcgacaaacc gctgccgcat attcgtcttg atgccgcgct
     7081 ggaattttta ataggagata aattgcgatg accgaaccgt taaaaccacg tattgatttc
     7141 gacggtccgc tggaggtcga acagaatcca aaattcaggg cgcagcagac ctttgacgaa
     7201 aatcaggcgc aaaattttgc cccggccacg ctcgacgaag cgcaggaaga agaggggcaa
     7261 gtcgaagcgg taatggacgc agcgttacgt ccgaaacgca gcctgtggcg caaaatggtg
     7321 atgggcgggc tggctctgtt tggcgcaagc gttgtcgggc agggtataca gtggacaatg
     7381 aatgcctggc aaacccagga ctgggtagcg ctgggtggat gtgccgctgg ggcattgatt
     7441 atcggcgctg gcgtaggttc tgtggtaaca gagtggcggc gcttatggcg cttgcgacag
     7501 cgcgcccatg aacgcgacga agcgcgtgat ttattgcata gccacggcac gggcaaaggt
     7561 cgcgcatttt gcgaaaaact ggcgcagcag gcgggtattg atcagtcgca tccggcgctg
     7621 caacgctggt atgcctcaat ccatgaaacg cagaacgatc gtgaagtggt cagcctgtat
     7681 gcgcatttgg tccagccagt tttagatgcc caggcgcggc gcgaaatcag ccgttcggcg
     7741 gcggaatcaa cgttgatgat tgcggtcagc ccgctggcgt tggtcgatat ggcgtttatc
     7801 gcctggcgca atctgcgttt aattaatcgc attgccacgc tgtatggcat tgaactgggg
     7861 tattacagcc gtttgcgtct gtttaagctg gtattgctga atatcgcttt tgccggagcc
     7921 agcgaactgg tgcgcgaagt ggggatggac tggatgtcgc aagatctcgc tgctcgtttg
     7981 tctacccgcg cagctcaggg aattggtgct ggacttctga cggcacgact cgggattaaa
     8041 gctatggagc tttgccgccc gctgccgtgg attgacgatg acaaacctcg ccttggggat
     8101 ttccgtcgtc agcttatcgg tcaggtgaaa gaaactctgc aaaaaggcaa aacgcccagc
     8161 gaaaaataat gcaatatcgg gtgctgaccg gatatcttta cgccgaagtg cccgtttttc
     8221 cgtctttgtg tcaatgattg ttgacagaaa ccttcctgct atccaaatag tgtcatatca
     8281 tcatattaat tgttcttttt tcaggtgaag gttcccatgc gtctggaagt cttttgtgaa
     8341 gaccgactcg gtctgacccg cgaattactc gatctactcg tgctaagagg cattgattta
     8401 cgcggtattg agattgatcc cattgggcga atctacctca attttgctga actggagttt
     8461 gagagtttca gcagtttgat ggccgaaata cgccgtattg caggtgttac cgatgtgcgt
     8521 actgtcccgt ggatgccttc cgaacgtgag catctggcgt tgagcgcgtt actggaggcg
     8581 ttgcctgaac ctgtgctctc tgtcgatatg aaaagcaaag tggatatggc gaacccggcg
     8641 agctgtcagc tttttgggca aaaattggat cgcctgcgca accataccgc cgcacaattg
     8701 attaacggct ttaatttttt acgttggctg gaaagcgaac cgcaagattc gcataacgag
     8761 catgtcgtta ttaatgggca gaatttcctg atggagatta cgcctgttta tcttcaggat
     8821 gaaaatgatc aacacgtcct gaccggtgcg gtggtgatgt tgcgatcaac gattcgtatg
     8881 ggccgccagt tgcaaaatgt cgccgcccag gacgtcagcg ccttcagcca aattgtcgcc
     8941 gtcagcccga aaatgaagca cgttgtcgaa caggcgcaga aactggcgat gctaagcgcg
     9001 ccgctgctga ttacgggtga cacaggtaca ggtaaagatc tttttgccta cgcctgccat
     9061 caggcaagcc ccagagcggg caaaccttac ctggcgctga attgcgcgtc tataccagaa
     9121 gatgcggtcg agagtgaact gtttggtcat gctccggaag ggaagaaagg gttctttgag
     9181 caggcgaacg gtggttcggt gctgttggat gaaatagggg aaatgtcacc acggatgcag
     9241 gcgaaattac tgcgttttct taatgatggc actttccgtc gggttggcga agaccatgag
     9301 gtgcatgtcg atgtgcgggt gatttgcgct acgcagaaga atctggtcga actggtgcaa
     9361 aaaggcgtgt tccgtgaaga tctctattat cgtctgaacg tgttgacgct caatctgccg
     9421 ccgctacgtg actgtccgca ggacattatg ccgttaactg agctgttcgt cgcccgcttt
     9481 gccgacgagc agggcgtgcc gcgtccgaaa ctggccgctg acctgaatac tgtacttaca
     9541 cgttatgcgt ggccgggaaa tgtgcggcag ttaaagaacg ctatctatcg cgcactgaca
     9601 caactggacg gttatgagct gcgtccacag gatattttgt tgccggatta tgacgcagca
     9661 acggtagccg tgggcgaaga tgcgatggaa ggttcgctgg acgaaatcac cagccgtttt
     9721 gaacgctcgg tattaaccca gctttatcgc aattatccca gcacgcgcaa actggcaaaa
     9781 cgtctcggcg tttcacatac cgcgattgcc aataagttgc gggaatatgg tctgagtcag
     9841 aagaagaacg aagagtaagc gcgaatatgc ctgatggtgc aacaccatca ggcatattaa
     9901 cttatgcttt cagtacagcc agagctgctt cgtaatccgg ctcggtggtg atttcatcca
     9961 ccagctggct gaaaatcaca ttgtcatttt cgtcaataac cacaacggca cgcgctgcca
    10021 gacctttcag taggccatca gcaattgcca caccgtaggc ttgcagaaat tcagcgttac
    10081 ggaaagtgga gagggtgata acgttgttca gaccttctgc gccgcagaaa cgagactggg
    10141 cgaacggcag atcggcagag atacacagca caacggtgtt gtcgatctca gttgccagtt
    10201 ggttaaactt acgtactgat gcggcgcaaa caccggtatc aatactcggg aaaatgttca
    10261 gcactttgcg tttacccgca aactgaccga gggtgacgtc agacagatct tttgccacga
    10321 gagtaaaagt ctgcgctttg ctacccgcct gcgggatgga attggcgact gtaaccgggt
    10381 tgccctggaa atgaacggtt tgtgacatga ttatctttcc tgtttacata tagttaacgt
    10441 cacacctagt ttatgctaac tgtcaataac acagcaaacg ctatttgcgc ttaatccgca
    10501 gaccaccgcg acaacaagga gtaaagatga gaatcgttaa ggtatttgag gaagcctggc
    10561 ccttacatac cccgtttgtg attgcccggg gaagccgcag tgaagcgcgc gtggtggtgg
    10621 ttgaactgga agaagagggt attaaaggca ccggcgaatg cacgccgtat ccgcgttatg
    10681 gagaaagtga tgcctcggta atggcgcaaa ttatgagcgt cgtgccgcaa ttagagaaag
    10741 ggctgacacg ggaggagttg caaaaaattc tccctgccgg cgcagcacgt aatgcgctgg
    10801 attgtgcatt gtgggatctc gcggcgcgaa aacagcagca atcgctggct gatttgatcg
    10861 gcataacgct tcccgagaca gttatcactg cacagacggt tgtcatcggt acgcctgatc
    10921 agatggccaa tagtgcatca acactctggc aggcaggcgc gaaattactg aaagtgaagc
    10981 tggataacca tcttatcagt gagcggatgg tggcaattcg caccgctgtg cccgatgcca
    11041 cgctgatcgt tgatgcaaat gaatcctggc gtgcagaagg gttggcggcg cgttgccagc
    11101 tattggcgga tttaggcgtt gcgatgcttg aacaaccgct tcctgcgcag gacgatgcgg
    11161 cgctggagaa ttttattcat ccgttgccga tttgtgctga tgaaagttgt catactcgta
    11221 gcaatctgaa ggcgctgaaa gggcgctatg agatggttaa cattaagctc gataaaaccg
    11281 gtggtctgac ggaagcgctg gcgactgaag cgcgtgcaca aggtttcagt ctgatgctgg
    11341 gctgcatgtt gtgtacctct cgtgccatta gcgccgcttt accgctggtg ccgcaggtca
    11401 gtttcgccga tcttgacgga ccgacctggc tggcggtaga tgtggaaccg gcgcttcagt
    11461 tcacgacggg cgaattgcat ctttaggatg ccagcgcagc aagtttgcca tcgcaaagag
    11521 atatttttcg ctggcttcgt cggaggagat gggcggaaac tctgcggtga tgcaatgcaa
    11581 gttgagatct gcacaccaac tgccgaaaga gccaggcgtt tcataaccga cgctggttac
    11641 cagaggcaat tcaaacgact gggccagcca ttcgcctaat tcgctgtgtc tgggatcttc
    11701 aatacaagcc agtggatcgt ggaatgagac cacccaggca ggttgaatgc ggtgtataag
    11761 ctggcacagc gcctgggttt caggttcgga gccaggtttg tcacctgtca gtaatacgac
    11821 atcacgttct tcagcggcgc tgttccagcg ataaaccgtt tcaccttcct tccagttcgc
    11881 cgccggaaag tttctgttta aatccacacc attagcattg gcccgtaacc ccagctggca
    11941 gccgtcagga ttcacacaca gcaccacatg atggcggcgc aaagaaggtg tcaaagtccg
    12001 cagagcacag gagagcgtga cgaccgaaga gttttcatca ccgtgagtgc cagccagaat
    12061 caaaccactt tcgcggctgg ctgcaggggc aggaaaccag attaacggcg cgcccaataa
    12121 tgaacgtccg taatgttctg ttccgggcgg aaatgcgccg cgttcggcgc gtgggcgggt
    12181 tacggtcatg atcgtctctg gttcaggaag tattacaggc agtgttgtgc aaattttgcg
    12241 gattatcaaa tccttttctt caacgctatg tcatcgatct tgtatcactt ttttgccgga
    12301 agttgtttgc attttcattg atgtgcagat attagaattg tcgtaatgtt atatacatgg
    12361 cgtattttat tcttactggt tctacacatg ttaatcgcat ctgattgatc agttcacctt
    12421 aataagttta ataaatcgta ctgcacatag tacagtacga tggtatctat ataattgatt
    12481 tacatgaaga atggcttatc gccatcagtc ccgggttgat tgatgcctat attctcacca
    12541 atgtgtttct caggtttatc gatgatatct gtaattaatg ctgcgacact cttacgtgat
    12601 acgatcgtac ctttgaatgg ttcattgcga gaggtaagct cataatcaat aatatcttca
    12661 tcagtcagct aggcagggcg aagaattgta tattccagtc cagaagcctc aattgcatcc
    12721 gcagccctgc ggaatggttt taggggttca ccaataactg cattattcca ttcgacaaat
    12781 tttcctggaa cttcatcata aataccgagg gaaagaacaa agatcagacg tttaacatca
    12841 caggctttca tcgcagcgat cactgaattt gcctgaatat ctaaatcttc ccccgtaagg
    12901 ttggcataca caatatcctg tccctgcatg gcctgcttca gtgctgcatg attcagtaca
    12961 tcccccataa taatctggct attagttgga tagggtttgt gtatttttgc tggctgacgg
    13021 gcaaataaag tttgtttaat agtttgttta tctgccagtt gattaatcac atggcgggct
    13081 atctgaccgc cagcaccaag aatcagtaca tttttcataa tatttctcat gaaacttatc
    13141 ccagatgggt gttgaaaaaa ggaataactt ttgccagcgc ctcctgaact gcttccggtt
    13201 tgtcatatag ttcataatgt gagaaatcgg gtaacgaaac taactgacga tcctgcgaaa
    13261 ccatcgcccg gccatagact tccagtccat cacgataagc accgaaggca ccgacttttt
    13321 caccaactac taccatcacc ggctgggtca gtaacacttc agtaaatgcg aaggcatccc
    13381 aggccagcgt tttctgtgca tgtgagaaca acatttttgc cgcacctccc ggttgttgcc
    13441 cgcgtggtgt tttatagtag tcagttgctt cataaacatc gcgttcggtc agtccatggg
    13501 cttttgctgc atccaggctg gcgggaagta attcgttgat ctgtaattct ccaccacgag
    13561 cctcttttgt tcgttgtgac gccattgcat tcaatacgcc aatcggatca taattgctga
    13621 aaccttcccg aaaaagacgc ccgatattaa cacccgtaat actcactaca gctttaattc
    13681 gtttttctgt cagcgcggca ttaatggcat agcc
//
LOCUS       DADLZM010000109        13524 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLZM010000109 DADLZM010000000
VERSION     DADLZM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.110"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..892)
                     /locus_tag="JNY73_003010"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="JNY73_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1114..1914
                     /locus_tag="JNY73_003011"
     CDS             1114..1914
                     /locus_tag="JNY73_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY8048942.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1992..2762
                     /locus_tag="JNY73_003012"
     CDS             1992..2762
                     /locus_tag="JNY73_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8048943.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2810..4168)
                     /gene="mltD"
                     /locus_tag="JNY73_003013"
     CDS             complement(2810..4168)
                     /gene="mltD"
                     /locus_tag="JNY73_003013"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY8048944.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4240..4995)
                     /gene="gloB"
                     /locus_tag="JNY73_003014"
     CDS             complement(4240..4995)
                     /gene="gloB"
                     /locus_tag="JNY73_003014"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY8048945.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5029..5751
                     /locus_tag="JNY73_003015"
     CDS             5029..5751
                     /locus_tag="JNY73_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8048946.1"
                     /translation="MKPARVPQTVVAPDCWGDLPRGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5748..6215)
                     /gene="rnhA"
                     /locus_tag="JNY73_003016"
     CDS             complement(5748..6215)
                     /gene="rnhA"
                     /locus_tag="JNY73_003016"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY8048947.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6280..7011
                     /gene="dnaQ"
                     /locus_tag="JNY73_003017"
     CDS             6280..7011
                     /gene="dnaQ"
                     /locus_tag="JNY73_003017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY8048948.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7144..7220
                     /locus_tag="JNY73_003018"
     tRNA            7144..7220
                     /locus_tag="JNY73_003018"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7178..7180,aa:Asp,seq:gtc)
     gene            complement(7351..8397)
                     /locus_tag="JNY73_003019"
     CDS             complement(7351..8397)
                     /locus_tag="JNY73_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY8048949.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8408..9283)
                     /gene="tssG"
                     /locus_tag="JNY73_003020"
     CDS             complement(8408..9283)
                     /gene="tssG"
                     /locus_tag="JNY73_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY8048950.1"
                     /translation="MEKFRLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGP
                     LPLHYTELARNRTESRRDPTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRF
                     AFYVASLSGQDLRESADSPLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQE
                     YVLHWIAVADDEITRLGMPAPSSVIGNGALIGQAVPDMQYKFRLVIGPLTLEDYRRFL
                     PGSNNLPVLTELVRAFSGYEYCWEIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDN
                     PVTGMIFEPEHYLAH"
     gene            complement(9400..11283)
                     /gene="tssF"
                     /locus_tag="JNY73_003021"
                     /pseudo
     CDS             complement(9400..11283)
                     /gene="tssF"
                     /locus_tag="JNY73_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            complement(11299..11793)
                     /gene="tssE"
                     /locus_tag="JNY73_003022"
     CDS             complement(11299..11793)
                     /gene="tssE"
                     /locus_tag="JNY73_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY8048951.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11790..12620)
                     /locus_tag="JNY73_003023"
     CDS             complement(11790..12620)
                     /locus_tag="JNY73_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY8048952.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12607..12984)
                     /gene="tagK"
                     /locus_tag="JNY73_003024"
     CDS             complement(12607..12984)
                     /gene="tagK"
                     /locus_tag="JNY73_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HAY8048953.1"
                     /translation="MLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLVTEAETLQDMVT
                     GGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNTVLPDLTRKEHR
                     IIGIDNHYRINPAQHGEIYHDKQ"
     gene            complement(13221..13391)
                     /locus_tag="JNY73_003025"
     CDS             complement(13221..13391)
                     /locus_tag="JNY73_003025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HAY8048954.1"
                     /translation="MNDDVKIALTLTRHEEAWWIINQSTEYCCTVNDQIVEPHHRMRL
                     NEGDLIEWGLSS"
BASE COUNT         3094 a         3520 c         3611 g         3299 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaata gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc
      841 cgctttcgac gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc
      901 ctattttgaa tttatttgtc attaaaccat caggatgcgt gatatgtcat ccgatttaat
      961 gttctcaata atgagcaaat ttctgaccgg tgtaagcact tgcttacata acaatataca
     1021 attgctcgtt gaaagagtga gctaaaatcc ctataacagt agaaccctcc cgagtgcgga
     1081 agggttgacg taatagaggt ttcaaagtca aaagtgcgaa aaaacaccta tgccatgcgc
     1141 tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg ggtcttttgc gagcatcggc
     1201 caggcattac cacctggaga accgttaagt accgaagagc gtattcggat cctggtgtgg
     1261 aacatataca aacagcaacg cgctgaatgg ttgtcggtat taaagaacta cggcaaagat
     1321 gcacatctgg tgttattgca ggaagcgcag acaacgccag agttagtaca gtttgcgacc
     1381 gctaactatc ttgccgccga tcaggtgccc gctttcgtgc tgccacaaca tccttccggt
     1441 gtaatgaccc tttcggcggc gcatccagtg tattgctgcc cgttacgtga acgagaaccc
     1501 attttgcgtc tggcgaagtc ggcactggtg acggtctatc cattacctga cacccgcctg
     1561 ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg gcgtggatgt ctatagtaag
     1621 cagttacttc ctattggtga tcagatagct caccacagcg gcccggtcat tatggcggga
     1681 gatttcaatg cctggagccg tagaaggatg aacgcgttat atcgctttgc gcgggaaatg
     1741 tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc gggcgtttgg ccgcccgctc
     1801 gattttgttt tctaccgtgg tctgaacgtc agcgaagctt ctgtattggt tacgcgcgct
     1861 tccgatcaca atccgctact cgttgaattc agtcccggca agcctgataa ataaggtatg
     1921 tcaggtctgc cacagggcag accaacgttt ggcgctgtcc ttttatactc aaacaacaaa
     1981 aggacagcgc aatgacaaca caatcccacc atgaccacgt agaaaagcag tttagctctc
     2041 aagcctgcga atatctgacc agtaccgtac atgcatccgg acgagattta cagcgcctgg
     2101 cggtgcgtct ggctgattat cctggtgcaa gtgtacttga tatgggctgc ggagcagggc
     2161 atgccagctt tgttgctgcg caaaacgtga gcgcggtggt ggcgtatgac ttatctgccc
     2221 aaatgctgga tgtcgtggca caagctgccg aagcccggca actgaaaaat atcaccaccc
     2281 gccagggata tgccgaaagt ctgccatttg ccgataacgc atttgatatt gttatcagcc
     2341 gttattctgc ccatcactgg catgatgttg gtgcagcact gcgtgaagtg aaccggatat
     2401 tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc tccgggtcat ccagtgcgcg
     2461 acatctggtt acagacggta gaagcattac gcgatacctc tcacgtacga aactacgcca
     2521 gcggtgagtg gttgaggtta atcaatgaag ccaatctgat agttgataat ttaattacag
     2581 ataagttacc gctggaattt tgttcatggg tcgcgagaat gcgtacgcca gaagcgttag
     2641 tagacgctat tcgcatttac caacagagcg catcgacaga ggtgagaacg tattttgcct
     2701 tgcagaatga cggctctttc accagtgata tcatcatggt agaagcacat aaagcggcat
     2761 aaataaaaaa ggcaccgggg gaatcggtac ctttttatta tctggtttgt caggaatctg
     2821 gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc tggttgcaga ttcgcagtgt
     2881 cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg gcgtttagca atgcttgaaa
     2941 gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct gttgtttgcc agtcgctgtg
     3001 cgctactgcc tgcaccaatc gtcaaacttt ggcctggctt cagcttagat ccgcgcagtt
     3061 tgttccactg ctgcaaatct ttggtgctta cgccgagacg tgaagcgatg cttgaaagcg
     3121 tgtcgccaga gcgtacggtg taaacacggc tgttaagcgg cgtattgtcg gcaaccagcg
     3181 tcgactgtac agcagcaatt tcgcctgaag cgagagattc acgcagttga tctgcatgct
     3241 tctttggcac catcacgtac tgcggcccac ttgcgcccag cgtggagcct ttcacgccag
     3301 cgttgaatgt cttcagcttg ctgacggaaa tccccgccat atctgcaacc ttcgccattt
     3361 caaccgggct actcaggtgc acacgcgcca gagcacggct ttcatcggtc gttggcagac
     3421 gtacgccata acgcttgctg tttttgagaa tatcactcaa tgccagcatt ttaggcacgt
     3481 actgcttcgt ttcctgcggc aacggtaacg accagaagtc cgtggatttc ccacgcgctt
     3541 tgttcgtttt aattgccttc atgacccgac cttcgccgct gttataagcc gctacggtca
     3601 gaagccagtc gccgtcaaac attttgttca gacgctgcat catattcagc gcggcagttg
     3661 ttgaagcaac aacatcgcga cgcgcgtcat aattgcgggt ctgtttcaaa ccataattgc
     3721 gccccgtgct cggaatgatc tgccagatgc ctgcggcatt ggcgccagac gttgcgtgag
     3781 gatcaaaagc gctctccact atgggtagta gtaccagttc cataggcatg ttacgttttt
     3841 taacttgccc tgctatccag tacatatacg gctctgcccg taaagttaca tcgtggagat
     3901 agctcttatt gcgtaaatat ttctgtttct gttcgcgaat ccggtcattt tccggaattc
     3961 ccatctttag ctcgtcgcca atgaaagccc acaagtcacc atctggcgcg atagacgtcc
     4021 catcgtccat ccatcgtgcc tgacttgtaa actttgctgc ttccccttga ccagctgcag
     4081 aaaggctctg tgcgtgctgt tgaacgttgc cggtactctg gcaacccacg agcaggacag
     4141 aggcgagtaa tatcgctttt gccttcatgt gtgtgtcaat agttgcttaa aagacgaccg
     4201 atcataacgg cgaacggagc cgatgacaag aaagttttat cagaacctat ctttctttga
     4261 ccttaaccat gcaaaacgct cttcaggttg ttgcaataat gtttcttcat taattacatt
     4321 aattaaatca atatcttccg ttcttaaaaa aacattaatt tgccgctcat ttttcagaat
     4381 tacgggtagt gttatttgat tttttgcccg taactcctta actttacgat aataatcatt
     4441 tatggacaaa tcgtgcggaa aaatactcaa agcaaacttc atatttgata acgtatattc
     4501 atgagcacaa cataccaatg tatcgtcagg taacgcactt agctttttaa gtgattgata
     4561 catttgtaaa gctgtccctt caaacaaccg accacaccca ccagaaaaca gcgtgtcgcc
     4621 gcaaaataga taaggtttac tgaagtaaca gatatgtcct aaagtgtgac ccggtgtggt
     4681 aattacacta aattcatgcc ctaaaacgaa ggcagtttcg ccatctttga ctacctgtgt
     4741 tgttccctta tcttgtgtct cttgtggacc atacaccaca atttgcggaa acttttccac
     4801 cagttctttt acgccgccaa cgtgatcgtg gtgatggtgg gttagaaata tggcctccgg
     4861 ttgccagtta ttggcggtaa tggcgtttaa tactggctct gcgtctccgg gatcgacaat
     4921 caggcagcga cctgcctcat cattcaaaac ccagatgtaa ttgtcatcaa aggcgggaat
     4981 actgttaaga ttcatagatt acctctcagt gtaaaacgga aggttgtgat gaaaccggca
     5041 agagtccctc aaactgtcgt ggctcctgat tgctggggcg atttgcccag gggagagctt
     5101 tatcgcaagg cgctggagcg ccagctcaac ccgtggttca ctaaaatgta tggttttcat
     5161 ctgcttaaga ttggcaattt aagcgcagaa atcaattgcg aagcgtgcgc ggtttctcat
     5221 caagtgaatg tttctgcgca aggaatgccc gtccaggtgc aggcggatcc acttcatctt
     5281 ccttttgccg ataaatccgt tgatatttgt ctactggcac ataccttgcc gtggtgcacc
     5341 gatccgcatc gtttattacg tgaagccgat cgggtactga ttgatgatgg ctggctggtg
     5401 attagtggct tcaaccccat cagtttaatg ggattacgca aacttgtgcc ggtattgcgc
     5461 aaaacctcgc cctataatag ccggatgttt actctgatgc ggcaactgga ctggctctct
     5521 ttgttgaatt ttgaagtgct gcacgccagc cgtttccacg ttctcccgtg gaacaaacac
     5581 ggaggaaaac tattgaatgc gcatattcct gcgcttggtt gcttacaact tattgttgcc
     5641 cggaaacgga ctattccttt aacgctaaat ccgatgaaac agagtaaaaa caagccacga
     5701 attcgccagg cggttggagc cacccggcaa tgtcgtaaac cacaggctta aacttcgact
     5761 tggtagcccg tatcttccag tgtgggattc atcgccgcgg cacgagccag ttcatcacag
     5821 cgttcgtttt ccgggtgtcc ggcatggcct ttaacccatt cccatttgat ttgatgctgc
     5881 cccaatgcag catcaagacg ttgccagaga tcgacatttt ttactggttt tttgtctgct
     5941 gttttccagc cacgtttttt ccagttatgg atccactggg tgataccctg gcggacatac
     6001 tggctgtcgg tactcaaaat gacttcgcaa tgttctttta acgcctccag cgcgacaata
     6061 gcggccatca actccatacg gttgttggtg gtgcgggtgt agcctgcgct aaaggttttc
     6121 tcgcgtccgc gatagcgtaa aatagcgccg taacccccag gtccaggatt acccagacac
     6181 gaaccatcgg tgaaaatttc tacctgttta agcatctctg gtagacttcc tgtaattgaa
     6241 tcgaactgta aaacgacaag tctgacataa atgaccgcta tgagcactgc aattacacgc
     6301 cagatcgttc tcgataccga aaccaccggt atgaaccaga ttggtgcaca ctatgaaggc
     6361 cacaagatca ttgagattgg tgctgttgaa gtagtgaacc gtcgcctgac gggcaataac
     6421 ttccatgttt atctcaaacc cgatcggctg gtggatccgg aagcctttgg cgtacatggt
     6481 attgctgatg aatttttgct cgataagccc acgtttgccg aagtagccga tgagtttatg
     6541 gactatatcc gcggcgcgga gttggtgatc cataacgcag cgttcgatat aggctttatg
     6601 gactacgagt tttcgttgct taagcgcgat attccgaaga ccaatacctt ctgtaaggtc
     6661 accgatagcc ttgcggtggc gaggaaaatg tttcccggta aacgcaacag cctcgatgcg
     6721 ctatgtgccc gctacgaaat agataacagt aaacgaacgc tgcacggggc attactcgat
     6781 gcccagatcc ttgcggaagt ttatctggcg atgaccggtg gtcaaacgtc gatggctttt
     6841 gcgatggaag gagagacaca gcagcaacaa ggtgaagcaa caattcagcg cattgtacgt
     6901 caggcaagta agttacgcgt tgtttttgcg acagatgaag agctggcagc acatgaagcc
     6961 cgtctcgatc tggtgcagaa gaaaggcgga agctgcctct ggcgggcata aatacatgta
     7021 aaaggcgcta aaaatagcga cttgggcgat ttttgcagca aacgattcaa aagatgagaa
     7081 aaatcgttga cgaaggtcga ggcaatccgt aatattcgcc tcgttcccaa cggaacacaa
     7141 cgcggagcgg tagttcagtc ggttagaata cctgcctgtc acgcaggggg gcgcgggttc
     7201 gagtcccgtc cgttccgcca atattcagaa agcccgaatc agtaatgatt cgggctttcg
     7261 tcgttttggt gaagtgagaa tttttcactc cgaagtaaaa cggatatgat ttaaaaaatg
     7321 gccagacatt ataaattctg gccacttctg ttactccagc gcatcccatt tctccagcag
     7381 ttctactgga atttcattta ttacctccga gacgcgtttt ccgaccaggc gttctgcctg
     7441 gcgcagcaaa ggaatggtcg ggctgctcgg ttcgctgtgc tcaaaccagc gacggatgac
     7501 acgcaggcgt tccagcgcgt cgtttcggtc gcggatctgc cggggttctg cgccggtcat
     7561 cggtgatatt tcctccgcga tatgcaccgg tagcgcaatc tgctctgcct gcatctgttc
     7621 agtatgaact atggtggcgt tttccggctg ctcagcctgt ggttgtggca ggatctccgg
     7681 cgtggtgctc tgcactgctc ccggtaacag ggccagcagt tgcgtcagac gggagaagtc
     7741 cggtgcctgg tcattcagcg tttcacgcgc atggcgttgc agccgctcag tggtctctgc
     7801 tgcctggcga aaggcatcca gcggcagtgt gccgcgcgct tccaggtctg ccagttgctg
     7861 gcgtaccgat tctggtgcca gcgcatccgc cgggcgtaat gcagatagcg aacgctccac
     7921 atcccgcacc tgaaggcgca tggcagcatt atttgacagt gtaatgccac gaatatccgc
     7981 cattacacct tcgtggtcca gcaacgccgc cagcgcgtta ctgcgtgcca ccgcagcatc
     8041 ttcggcggtg atatcttcag tcgcgagcag ttgaggatgg atggcgtccg ggtacgtgga
     8101 gcacaacgtt tccagcagcg tgagcgcctc cgacagtccc ctggcaccgg cctgctggat
     8161 acggcagcgc agcaggacaa ccagaatgcg gatatctttg ctgcgcatca gcagacgatg
     8221 ggcgtcccgc tcaagctccg cccagttaat gttttccggc gtactgacaa aatcgccata
     8281 ttgcgcttct tcacgcggtg cggcgcgggt gaacagcagc aggtattccg gatcgtactc
     8341 cagatccggg ccgcaggggc attccgccga aattgggtca gacaggaata tttccattta
     8401 attatcctca gtgcgccagg tagtgttctg gttcgaaaat catgcccgtc accgggttgt
     8461 cgtgcatggc tttacccgcc catgcagtcc aacccagttg gtacggacca ccggtaacag
     8521 ccggaggggc ggcgtggggt tttagctgta gctcaatttc ccagcagtat tcataaccac
     8581 taaaggcgcg gaccagttct gtcaggacgg gcaggttatt gctgccaggc agaaaacgtc
     8641 ggtaatcctc cagcgtcagc gggccgataa ccagccggaa cttatactgc atgtccggca
     8701 cggcctggcc gatgagtgca ccattgccaa taacggaaga gggggcaggc atccccagcc
     8761 gggtgatctc atcgtccgcc acggctatcc agtgcaggac atactcctgt atccggaaag
     8821 gtacaccgaa atagtgcgcc agcgtggtgg ccagaccgtc cggattgcgg gactcacgca
     8881 ccagatgggc cgatgccgcc agacgagcgt gatctggcaa cgggctgtca gcgctctccc
     8941 gcaaatcctg cccgctgagg ctggcgacat aaaaggcaaa gcggtcatgt tgcggtttat
     9001 ccagaccgcc cccagcggac tgggcgctgc gccatgcctg gtaaaactgc gtcagccagc
     9061 ggtgatgaaa aagatcagaa aaacgggtca gcgtcggatc gcgtcggctt tcagtccggt
     9121 tacgcgccag ttcggtgtag tgcagcggca gtggaccgtt cggcccccac agcccgaggc
     9181 tgtacagact gatgtgcagg cgcccttcct gccagcccac actggcgatt tctcgtggcg
     9241 caaaggtcag cgcgggcgtt tgtccgaggc gaaatttttc catccgtggt tgctaggtgt
     9301 tgcccgccgg aacggtacac aactgcacat ccacgcggcg catcaggttc agaaaaccgt
     9361 aatgccaggg gggttcctgc gcctgagtga aatcgttgct catacgcttc ccctctgtcc
     9421 cgccctgaca ggccaggtca taattttgcc gcgctgcatg ctgtgcagcg tcatctgcga
     9481 gaaggtatta atggaaacat ggcgggcgat atagtgttcc agcaccaggc cgaacagata
     9541 cggactgatg ccggaaaaac cttcttcatc caccgtcagt tcacaactga cgccacgccc
     9601 atagaccagt agtccggaac cgggcagacg gcgggtgacc ggggtcgttt tacaaccaat
     9661 caggctgcgc acctggcgcg attgcggact gtcatgagca ggaataaaca ggttcagcag
     9721 atctcgcaac gcctggccgc cggtacggtg atccagatcg gccagcggca gataattaaa
     9781 agataactgg cggatcaacc gccatgccat ctcacgttca gccagcgggg gctgcggcga
     9841 gcgtggcggg cggataagcc ctacgcccgc caccgggatg gctgcatcca cggttagatc
     9901 gtcccggcca ttgcgggcaa taagacaggg cagatcgcgg ttagtgacca tggccgtgat
     9961 ggtgatgtgg cgcagatttt ccgggtacgg cgcttcatac tggtcaacca gcgacaggaa
    10021 cacttccgag cccgtatagg gggtacgggt gccgtaacgg cgggcgtttt ctgataagcg
    10081 gcggggctcc cggcgcaggg aaaaataacg tccgtgattg ccttcatcat tattgcgggt
    10141 gtggtagagc gggcgaaagg ccatcttccg tgtggtgtcg gtttccagcc cttccacttc
    10201 ctggaccgag aacacctcat aatccagcgg atgggttctg tccaccacca gatgctgttc
    10261 cgtcgtgctg tgcgtgacat cgatccgggc ggtgacgcgg ggaaacaggt tgataaccgg
    10321 ggtacagaac aggctgaact gtgccgcatt tgtctggtgg atcagtcagt ccggcggcag
    10381 gcggttcagc aggataacaa tctccgccac gccaccgtca attttctgta atccggcaga
    10441 caggcctgtc ggggtgaaaa aatagaagcg ttccggacag gcaaaatatt catgcagcag
    10501 gttatgaccg tgaaacacgt tccacgccag cggcagcagc ccctgatccg gctccaggcc
    10561 ttcgtacata acgggctgtt gcagattcac atccagcgcg ccgtcaaaat gccccggaat
    10621 gccagccagg gtcgcgatgg cgctggtatg tagaagttct aacaggtgtg aggccgtacg
    10681 ctcctcgccg cagagataaa acggcagcct gtccagtccg gcaagctgac tgaacgttag
    10741 ttcaccaaag gtgcgcaggg taatacgcag cgccccggcg acgtgaatgt tggccggcag
    10801 gtagcggtgc agggcaggca tatccggtgg cgcggcggtc agtcgcgcct cttcgatggc
    10861 gagcggccac agcgttacat cctggctgct gcgaaactgg caggcagtgc tttcgccttc
    10921 cggaatggcg gagaaaaagg ctgtgtcgcg cgggacggta aatcctttcg ccagatcgcc
    10981 ttcttccgta tcgggatgca actgcgccac gctcattgag ggcgtgggtg tgacgtaatt
    11041 ggggctgacc acttccagcg ggcgctgggt gaaacggggg aattcagcgt caatttttag
    11101 ctgcgtgcgg gcggtcagga agctgaaggc ctcgaccatt cgctctgcat acggatcggc
    11161 aatatccgtt ccctgcatgc ccagccgggc ggcgactttg ggatgacggg cggcaaattc
    11221 ggctcccgtt tcccgcagat agctcagttc gcggttgtag tatcccagta gccgtggatc
    11281 catcggtctc tctctgaatt aaaaaaacgt aatgcgactc tgctccatat ccagcgcgct
    11341 acgtacccgg aatgcagtcg gatacggctg cgtgaggatc tcgccgcgaa tttcaaactg
    11401 taatgtgtta tggccactct ggcggcgctc atcctgaagt gggatgattt gcagggtgga
    11461 cgctttcagc cggggttcaa accgggtgat ggttcgcaaa atggccctgc ggatatcgtc
    11521 ccacttgtgc tcatacagaa agctgcccgc cagtggcggc aggccgtagt taagtacgga
    11581 cgctgccgcg tacggataac gtgcagcatc aatatcatct tcatggctga cggtgttgag
    11641 cagaaatgac aaatcccggc ggatgatctc cttaatctga accgggctga cgctgatacc
    11701 gtggtcgcgt ttctggtttg gggcgtcgtc acacaggcgg tcaaacagcg tgggcagcag
    11761 gtgattgcct ggacgctggc gggaagcgct cacgcgtcgc ttccttcagc ggtgttaaag
    11821 gtacaggtgg acaaatccag caggctgata tcgccgtgac cggtgagcca caccctctga
    11881 cccagcgcct gtaccagtgt ctctcccgga ccgtcctgcc agaccgtttc ccggcacaga
    11941 cgcagggagt cggcagcctt ttcagagccg ctgtagcggg gaaacagcca ggcactgtgt
    12001 gtatcgccat tgttcagggt gacgttgacg ggtttccaga tcaaatcgat cagatgcgca
    12061 ggccgcggcg atttcagcga acagatttgt gaaaacggca gccagatata tgctccgccc
    12121 gcgatcagtt cgagtaccgg accggtacgg gagtcgctgt cgctaatcca gtcaaatgcg
    12181 ccgccattcc actgcccgga agtgtctgtt atggcctcaa gcgcgcggct gcggtgagca
    12241 tcagcttcct gcgcttcacc acgggcgtta caggcgaggg tggtcagcag atcctccatc
    12301 caggcgggcg gcgggagaat aacaccgggc cgctgttcgc cctgaaagca ggcgtggcgg
    12361 tatatctcac agcggatcag ctcgccaaat acctgcgctt cgcgagtata atttgcgtcc
    12421 atccgggcgc atagctggat ctgttgcagt gcccgcgacc agtcgcctgc cacgcacaat
    12481 aactgaaaca ggctgtggcg gcgggaggcc tgcgctgggt gcatcctgac ctgttgctct
    12541 gccatctgaa tgccctccac cacggaataa tcccgcagca gcgccgacaa ggtaatcggt
    12601 agctcgttat tgtttgtcat gatatatctc tccatgttgt gccgggttta tccggtaatg
    12661 gttgtcaatg ccaatgatgc gatgctcttt ccgtgtcaga tctggcagta cggtgttatg
    12721 cgctgtttcc ggggcgtatt ccggggaaag taaatgcagg atatcagggg gagtcttttc
    12781 gacaggccag atagtttcat cttcgccagt ggcatccagt acattgagaa tagcgtcaat
    12841 gcttaaccca ccagtgacca tatcctgcaa tgtctcggct tcggtgacca ggtcacgcaa
    12901 tgaatccaga tcctgcgagg agggctcttc gttaaaagga aaaggatcag catggcgatt
    12961 aagatcttgt tcgggagata acaacgcctc cggtattcct gataaagtcg ggtcagggca
    13021 tttccttttt ccgcaccttc tccggtggag gaggctccat gaacaggaat gatatcgaat
    13081 ggattttgtg atgtcagatg ctgctgcctg aaccaatcca gatccagata ttcggttatt
    13141 gcttccgacg tgatggcagt ctgcgatatc gtttttactg tgctcaggtc cggagatgat
    13201 ggattgccat gtacaaggca ctatgaagat aacccccatt ctatcaggtc gccttcattc
    13261 agacgcatac ggtgatgagg ttccacaatc tggtcgttta ccgtacagca atattcggta
    13321 gactgattga taatccacca tgcttcttcg tggcgggtca gagtgagggc aatctttaca
    13381 tcatcattca ccttaaaaac aggaatccag ggagaggtaa cggtgaagat aatttgtgtt
    13441 ccttcagtcg cgtaaattgt gttatcggcg atcccgtgcg cctgtatttt ttggagtccc
    13501 cacgtaatat ttggtttcat tgtg
//
LOCUS       DADLZM010000110        13174 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLZM010000110 DADLZM010000000
VERSION     DADLZM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.111"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            19..255
                     /locus_tag="JNY73_003026"
     CDS             19..255
                     /locus_tag="JNY73_003026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048955.1"
                     /translation="METHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLVAMWEVR
                     GNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(321..553)
                     /locus_tag="JNY73_003027"
                     /pseudo
     CDS             complement(321..553)
                     /locus_tag="JNY73_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
     gene            complement(663..1433)
                     /gene="yafV"
                     /locus_tag="JNY73_003028"
     CDS             complement(663..1433)
                     /gene="yafV"
                     /locus_tag="JNY73_003028"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY8048956.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1587..2060
                     /gene="ivy"
                     /locus_tag="JNY73_003029"
     CDS             1587..2060
                     /gene="ivy"
                     /locus_tag="JNY73_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY8048957.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2103..4547)
                     /locus_tag="JNY73_003030"
     CDS             complement(2103..4547)
                     /locus_tag="JNY73_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY8048958.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4786..5364
                     /gene="lpcA"
                     /locus_tag="JNY73_003031"
     CDS             4786..5364
                     /gene="lpcA"
                     /locus_tag="JNY73_003031"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY8048959.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5570..6337
                     /locus_tag="JNY73_003032"
     CDS             5570..6337
                     /locus_tag="JNY73_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY8048960.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6308..7048)
                     /locus_tag="JNY73_003033"
     CDS             complement(6308..7048)
                     /locus_tag="JNY73_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY8048961.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7340..7953
                     /locus_tag="JNY73_003034"
                     /pseudo
     CDS             7340..7953
                     /locus_tag="JNY73_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8129..8626
                     /gene="rayT"
                     /locus_tag="JNY73_003035"
     CDS             8129..8626
                     /gene="rayT"
                     /locus_tag="JNY73_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY8048962.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(8843..10582)
                     /gene="flhA"
                     /locus_tag="JNY73_003036"
     CDS             complement(8843..10582)
                     /gene="flhA"
                     /locus_tag="JNY73_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY8048963.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            10527..11312
                     /gene="lafU"
                     /locus_tag="JNY73_003037"
     CDS             10527..11312
                     /gene="lafU"
                     /locus_tag="JNY73_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY8048964.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11383..12438
                     /gene="dinB"
                     /locus_tag="JNY73_003038"
     CDS             11383..12438
                     /gene="dinB"
                     /locus_tag="JNY73_003038"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY8048965.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12490..12783
                     /gene="yafN"
                     /locus_tag="JNY73_003039"
     CDS             12490..12783
                     /gene="yafN"
                     /locus_tag="JNY73_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY8048966.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12786..>13174
                     /gene="yafO"
                     /locus_tag="JNY73_003040"
     CDS             12786..>13174
                     /gene="yafO"
                     /locus_tag="JNY73_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY8048967.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
BASE COUNT         3186 a         3451 c         3396 g         3141 t
ORIGIN      
        1 aagggatata aagtaacaat ggaaactcat gcattatctg acgattgttt agattgtgta
       61 tatgattggt atgcggaaaa aaatggacaa ttttttagtg caacgagtcc agaagagtta
      121 cttggattag ttgcaatgtg ggaagttcgt ggtaatgact ggagagctaa tttgaatgag
      181 ataaatactt atcaaaaagc catagaagaa tccccgtttt ttgataaaaa cggcaatgaa
      241 atagagtctg actaaatttt attgatatag caacgataat aaccggaaat ttcttccaga
      301 tctttccggc tttttattat ttaactttcg ttcagcacac taaacatccc gtctcgcatc
      361 cccttaactg actgtttaac ctgataaagt tccctgcgca gttcctgtac ttcgttgtta
      421 tcccaagcag gatcaaacat ccctccgaga tcttcacggt aacgcccgtg tcgatctcaa
      481 atcccgcttt tctcagccat ttggcgctga ttttgatcac cggcgtgtcg ctgcggttgg
      541 gcagataacc cacggtacag cgacgctgtg ttttggaaat ggtgacttct ggcgtaatat
      601 tgtgtttaag ttaggggctt tcgtaatcta gcccagcccc cggccctaaa cacaaccccc
      661 actcaccaca acctaaactc atccgcatcc cgccatgccg gaaacttttc tctatattcc
      721 cgcaatgcca ccatcgacag ctccgcatca atacgcgttg ccttatgcgc gtcggcggta
      781 gcgataatct cgccttgcgg attaatcacc cggctgtcac cgcgataatg gcagccgttg
      841 ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat cgcgcgggcc
      901 gtcagcaatg cctgccagtg cagagagcgc ggagctggcc agttggcgac gtacagggcg
      961 aggtcataat cgttgagatt gcgcgaccac actggaaaac gtaagtcgta gcacaccagc
     1021 ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc tttataatgc
     1081 agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac cgtgccgccc
     1141 ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc aacactgcct
     1201 gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agttcactac gtcatcttgt
     1261 gcaagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctc cggtagaacg
     1321 atcacatcgc gcccggtaat accttccagt tgacgatcaa aatggcgcag gttggcagga
     1381 ccatccaccc acaccaacgg ttgctgcaaa agcgtaatct tcaaactagg cacggtgtac
     1441 aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt gccttagcgc
     1501 agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat tatcgtgtta
     1561 tcgccaggct ttaggaggtt aataacatgg gcaggataag ctcgggagga atgatgttta
     1621 aggcaataac gacagtcgcc gcactggtca tcgccaccag tgcaatggcg caggatgatt
     1681 taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcgtttaat cagatggtgc
     1741 aagggcataa gctacctgca tgggtgatga aaggcggtac ttatactccc gcacaaaccg
     1801 taacgttggg agatgagacg tatcaggtga tgagcgcgtg caaaccgcat gactgtggct
     1861 cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg ctgttctcgg
     1921 ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac gatgcgcttt
     1981 cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa aaccatccgg
     2041 atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc tccgttattc
     2101 aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt cgtcgccagc
     2161 tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc cgctttcacc
     2221 agaatagcgg cttcatcttt atcaatcagc cccttcgtca gcgcgttgtg cgccagttca
     2281 tccagacggg taaatggcag atttttaccc agctctttac agatccgctg atgaatcgag
     2341 tcggcggcaa tcacatccac caacgcctct tccagtaaac caaccgggtt atgctcgctt
     2401 ggcgtcaggt actgaccgcg accaatacgt gaacgagtgg cgttcggcac ttgtaaaatc
     2461 ttcgccactt tatgatccag cttgtcagac ggtgccagat aatgacgtcc ggtcgggaag
     2521 atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag taaatcgtcc
     2581 atcgcctgtt ctgcctgata cagcgcatcc tgtacgcccc agtgcaccag cggcagatcg
     2641 gcttcattac ggccttcgtc gtcataacgc ttcagaacgg cagaggcgag gtaaagctgg
     2701 cttaaaatat cccccagacg tgccgagata cgctcacgac gtttcaggct gccgcccagc
     2761 agtgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt caggtgctga
     2821 tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg cgtcaggccc
     2881 agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa caacagttta
     2941 tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac gtacggatgg
     3001 caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt agccccttca
     3061 acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct ttgcccgagc
     3121 ataatgcctt taccgccggt aatatccatc gcatcaataa tcgactgctg cccgcggtgg
     3181 gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag cataatgccg
     3241 taggtaatca gcgatgccgc agcatccatt acataagcgt tcccggcaat acgcgccagc
     3301 ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg aatgtgagaa
     3361 tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga aggcagggtg
     3421 atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc ggccattttc
     3481 ggcccgccga ttatgtaatc gatcggcacg aagacatctt taccgcgcgt cggtccgttc
     3541 tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg cgtggtggtt
     3601 gggatcagcg cacaggtaat gcctaaatct tctgcaccgc cgagtaattt ttccgggtcg
     3661 gagagtttaa acgccagccc aagcacggtc gcaatcggtg ccagcgtaat gtagcgtttg
     3721 ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat gcagacaatc
     3781 ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggctggtcag tgcaaagcag
     3841 gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc gtcagtgccg
     3901 taatgttgca acagttcgcc cgggcctaat gagtttggca cgccgacggt aatcgccagg
     3961 atcccgctca cgccagagag tttttgcagc acacgagact gggcataagc tgagaactcc
     4021 agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt aaggtacgcc
     4081 cacaactccg gcggcagatc cgccagctca tgagtaatct ggaaatcatt cgccatccgg
     4141 caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cagcggtcag gcgcggctgc
     4201 ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc ctcccaccag
     4261 gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat caccttacgg
     4321 aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa gttaaatggc
     4381 acgaggataa tggtcagagg caccagtacc cacgccgacc acagaccagc aacgccgagg
     4441 gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg gtgatagaac
     4501 aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac gaaaagcccc
     4561 ttacttgtag gaggtctgac cactgtgatg atatggttgt agtggatgta aaaacattta
     4621 gcaatgtgtt tacaatataa ttacaacaaa gctcacattg ttgctgtttt tatccgcact
     4681 tcaggtcaaa aagtcctggt catagcacct gcccgtactt ctcgcttttg gcggtatccg
     4741 gtacactgca ttttgtctat tacatttatg ctgaaggata tcctcatgta ccaggatctt
     4801 attcgtaacg aactgaacga agcggcggaa acgctggcta actttttaaa agatgacgcc
     4861 aatattcacg ccattcagcg cgcggcggtc ctgttagcag acagctttaa agccggtggc
     4921 aaagtgcttt cctgcggcaa cggcggttcc cattgcgacg ctatgcactt tgccgaagag
     4981 ttgaccggtc gctaccgtga aaaccgtccg ggctacccgg cgattgctat ttctgacgtt
     5041 agtcatattt cctgcgtcgg taatgatttc ggtttcaatg atattttctc ccgctacgtt
     5101 gaagcggtag gtcgcgaagg cgatgtactg ctggggatct ccacctccgg taactctgca
     5161 aacgtgatca aagcgatcgc agcggcgcgt gagaagggga tgaaagtgat caccctgacc
     5221 ggtaaagacg gcggtaaaat ggctggcacg gcggatatcg aaattcgcgt tccgcacttt
     5281 ggttatgccg accgcattca ggagattcac attaaagtga tccatatcct gatccagtta
     5341 attgaaaaag agatggttaa gtaagtctgg cgtaggccgg ataaggcgtt tacgccgcat
     5401 ccggcacttg tgcgctgatg cctgatgcga tgctgacgcg tcttatcatg cctacaaatc
     5461 tgtacccgaa ccgtaggccg aataattcgt ttacgccgca tccgacctga aaattcttaa
     5521 atcaatcttc gccggggtcc atgcgctccc gctgttgtgg aggttaccca tgtgcgaatt
     5581 gctcgggatg agcgccaacg tccctaccga tatctgcttt agtttcaccg gacttgtaca
     5641 gcgtggtggt ggaaccgggc cacataaaga tggctggggc attacctttt acgaaggtaa
     5701 aggctgtcgc acatttaaag atccacaacc cagctttaat tcccccatcg ccaaacttgt
     5761 ccaggactac ccgataaaat cctgttcggt ggtggctcat attcgccagg ctaatcgggg
     5821 cgaggtggcg ctggaaaata ctcacccatt tacccgcgag ttatgggggc gtaactggac
     5881 ttatgcccat aacggacaac tgacgggcta caaatcactg gaaaccggca acttccgccc
     5941 ggtcggtgaa accgacagcg aaaaagcctt ttgctggctg ctgcataaat taacgcagcg
     6001 ttacccgcgc acgccgggca acatggcggc agtgtttaaa tatatcgcct cactggcgga
     6061 tgaactgcgg gcgaagggcg ttttcaacat gctgctttcg gacgggcgct atgtaatggc
     6121 gtattgctcg actaatttac actggatcac ccgccgcgcg ccgtttggcg tggcaacgtt
     6181 gctggatcag gatgtggaaa tcgacttcag ctcgcagacc acaccgaatg atgtggtcac
     6241 ggtgattgcg acacagccgc tgacgggcaa tgaaacctgg caaaaaatta tgccaggcga
     6301 atggcgctta ttttgcctcg gggagcgtgt agtttgatgc cagttgtggc tgcaccactt
     6361 cgtggcttaa cggcttgctg accacgtaac ggccattggc cacagaaacg gtcggtggct
     6421 tacgggtttg ctcaaagtag tcgtagcccg gcttcagctg ctcccagaag tccttaaagt
     6481 tggaatattt atggcgcttc atattggcgt cggtcatgcg gaatgggtaa atactcacct
     6541 gcacgctcgg ctgaccaaac accagcgcac cagtaacgaa ctggaagatc tcatcaatac
     6601 cctgattggt cattgcgtag cagccgatgg atacacaatc gccgtggatc atcaggtatt
     6661 tcccttcata accatgcgca cggtcataag cattggggaa tccaatatta atcgctttgt
     6721 agtaacggct gtctggtttt aactgattac gctggacgct ataaaatcct tccgggcttt
     6781 tgaaatcgcc ctgacgctgt tttggcccta agccgccgga atatttacag attttataac
     6841 tgtcgagcag ttgatattgc tcgcccattt tgacgtaaag atcgagcgta cgttcttcct
     6901 tgaagatctg gatgtagaca ggggatccca tcaactgctg cttatactct ttgctcactg
     6961 gcgtggtcga gctactgctg cccagcaaac cggcaaacga aacgcacggg atcaacaaca
     7021 tcgcaagaat taatgcgatt ttacgcatac tgcttattcc ttgataaaac ggttacacac
     7081 gccaggacgg caaaatggat cccaaatcgg aatagtctgg atttggaagg ctcacattat
     7141 caccaaaaga gttttacgca agcctgtcgg cgctgggttt acaaatttag cacaggggca
     7201 gttttcaata gtccgaaaag cgcatgtatc atttatttcc taatattatg aaatctcaat
     7261 agtaatatgc aaataagcaa ttaataatcc cgtttactcc tccggtcttt taaaattctc
     7321 accttcattc agccggttta tgtctttcat gtcttcgttc ttgctcggca gatttttgca
     7381 tccaggcgtg ttttcgttat gcgttttgct ccctttactc gccagcgcca ccacaaccgg
     7441 gagcagataa aacagcatcc agaatggttt cctgcgccgc tcaaggcgag tgacagacgc
     7501 tggcaggagc tggcggaaaa caaccacttt ttaagcagcg atcacctgca taacatcacc
     7561 gaagtggcga ttcaccgcct ggagcagcag cttggcaagc cttacgtctg gggcggcacg
     7621 cggcccgatc aaggcttcga ttgtagtggg ttagtttttt atgcctacaa caagatcctt
     7681 gaggcgaaac tcccgcgcac ggccaatgag atgtaccact accgtcgggc aacgattgtg
     7741 gcgaataacg acctgcgccg gggagatttg ctatttttcc atatccacag ccgcgagatt
     7801 gccgaccata tgggggtgta tctgggcgac ggtcagttta ttgagtcact gcgcaccggg
     7861 gaaaccattc gggtaagccg gttagccgaa cctttctggc aggaccattt tttaggcgca
     7921 cgcaggattt tgacggaaga gatgattttg taggccggat aaggcgttta cgccgcatcc
     7981 ggcggttgtg cacggatgcc tgatgcgacg ctgacgcgtc ttatcatgcc tacaaatctg
     8041 tgcgtttttg cgagccgctt tcccgatata acacagcgtc attgtcggcc tttccttttc
     8101 ctcttactgt tgagaaaaag gagtcgctat gtcggaatat cgtcgttatt acatcaaggg
     8161 gggaacatgg tttttcacgg taaatttacg caatcgtcga agccatcttc tgaccaccca
     8221 gttccagacg ctccgtaacg ccattattaa cgttaagcga gacagacctt ttgaaattaa
     8281 cgcctgggtc gttttgccgg agcatatgca ttgcatatgg acattacccg aaagcgatga
     8341 tgatttttcc tctcgctggc gggagattaa aaagcagttc acccatgctt gcggattgaa
     8401 aaatatctgg caaccgcgtt tttgggagca tgccattcgc aatacaaaag attatcgcca
     8461 tcatgttgat tatatttata taaatccaga gaaacatggc tgggtaaagc aggtgagtga
     8521 ttggccattc tcaacgttcc atcgcgatgt cgcgcgaggg ttatatccca tcgattgggc
     8581 gggagacata acggatttaa gtgccgggga gcgtatcatt ttataactgt gcgctggtgc
     8641 cggatgcgct tacgcttatc cggcctacaa atcgtgcttt aacggtaggc cggataaggc
     8701 gttcacgccg catccggcag ttatgcgcag atgcctgatg cgacgctggc gcgtcttatc
     8761 atgcctaaaa acttgtgcct gaacggtagg ccggataagg cgtttacgcc gcatccggca
     8821 attgtgcacc gatgccggat tcttacatca acgcccccat aatcttcaac tccagctcat
     8881 ccggcacttc gttatacgac agcacatgca gccccggcgc aaacaacctt gcataacgcg
     8941 ccagcaaagg gcgtagctgc ggcggcacca gcagcaccgg atctttcccc gccgctttca
     9001 tctgctcttt cacctgtggc atcgtgctct ggaactggtt gagcatattc gggtcaaccg
     9061 gcacgctgtc gagcatcact ttgccggcct gttgcgcctg attcactacg ttggtcagca
     9121 gattttccag ctcattattc agcgtataca ccgtcagctc ctgcttgcga acgaacggat
     9181 gagtaatgct gcgccgcagc gccaggcgca catcggccgc cagcagaata tgatctttag
     9241 tcaccgtgct actggcgacc agcacggtgg cgatggtgac gatatcgcgc agggaaacgc
     9301 cttcggtcag cagcgcccgg tacactttca gcaattggct gtaattgagc gccgcgctta
     9361 aatcctccgc caggcgcggt gccatcgacg acaaacggtt atgcaactgc gtgatgtcat
     9421 catagttaaa caaatcagga atatagctgc gcacaatctt gttcacatgg gtggcaatca
     9481 cactggcgct gtcgatcacc tgatacccca tattcagagc cttcgctttc tgcgcaggct
     9541 ggatccaggt gaccggtatc ccgtacgccg ggtcgttccc cagcacgccg tcaatctcgc
     9601 cgtaggtttc gctggagggc agcgccatca gtttatccgc cggaatatcc gcttcatcag
     9661 ccttaatgcc gttgatgaaa atagcgtact ggctgggctt gaggcggaag ttttcccgaa
     9721 tgcggatctc cggcagcagc acgccgttac cgtcggaaat cacctgccga acgccgcgaa
     9781 tccgctgggt gagcgggttg ccctgcgctt tgtccaccag cgccaccagc ttgtagccga
     9841 ggcttaagct gatgggctcg atcagcggaa tagtttccca actgacctgt tgttcgctgg
     9901 tttcagtgat ggtgcgggtc agcgtttcga ggcttttctc ttccgcctcc gccgcctgcg
     9961 ggcgtttgct catccgccag ccggtaaagc caagcagggc gctgaacagc aaaaacggca
    10021 gatgcggcat ccccggcacc accgccagca cgaacatgat cccggtggcg gtgtagagca
    10081 ccgacgggct tgccagcaac tggtggcgca cgtcatgggc gatatcgccg ttgtcgctga
    10141 cgcgggtgac gataatcgcc gccgcggtgg agagcagcag ggaagggatc tgcgccacca
    10201 ggccgtcgcc gatggtcatc agcacgtatt gctggaaggc ggcgtcggcg ctcaggttgt
    10261 atttgaagat gccgatacag acgccgccgc tcaggttgat cgccagaatc atcatcccgg
    10321 cgatggcgtc tccgcgtaca aacttcgatg ccccgtccat cgcgccgtag aaatcggcct
    10381 cgctggcaac atctttacgc cgggtttgcg cctgcgcttg gttgatcaat ccggcgttaa
    10441 gatcggcgtc aatcgccatc tgtttgccgg gcatcgcgtc cagggtaaag cgggcagaaa
    10501 cctcggaaat acgctcggcc tctttggtga cgacgataaa gttgattgtc aacagcgtca
    10561 gtaaatccga gcgtgaaagc attatcgccg cgctgcacgg gcagtcgatt tttaacggtg
    10621 gtgggctgtc gccgttaaat aaaatcagcc cgtctcatcc gccaaaacct gccaccgtgg
    10681 cggtgccgga agagacggag aaaaaagcgc gtgatgtgaa tgagaaaacg gcgctgctga
    10741 agaagaaatc cgccaccgag cttggcgagt tggcaaccag tatcaacacc attgcccgtg
    10801 atgcgcatat ggaagcgaat ctggagatgg agattgttcc ccagggatta cgcgtgctga
    10861 ttaaagacga ccagaatcgc aatatgttcg aacgcggcag cgcgcagatt atgccgttct
    10921 ttaaaacttt gctggtggag ctcgcgccag tgttcgactc gctctataac aaaattatta
    10981 ttaccgggca taccgatgcg atggcctaca aaaacaatat ctacaacaac tggaaccttt
    11041 cgggcgatcg cgcgctttca gctcgtcggg tgctggaaga ggccggaatg ccggaagata
    11101 aagtgatgca ggtaagcgca atggcggacc agatgctgct ggacgcgaag aatccgcaaa
    11161 gcgcgggcaa tcggcgtatt gagattatgg tactgaccaa aagtgcgtcc gatacgctgt
    11221 atcaatactt tggtcagcat ggggataaag tggtgcagcc gctggtgcaa aagctggata
    11281 agcagcaggt gctttcgcag cgaatgcgct aaatgctgaa tctttacgca tttctcaaaa
    11341 cctgaaatca ctgtatactt taccagtgtt gagaggtgag caatgcgtaa aatcattcat
    11401 gtggatatgg actgcttttt cgcagcggtg gagatgcgcg acaatcccgc tctgcgcgat
    11461 atccctattg ctattggcgg cagccgcgaa cgtcgggggg tgatcagcac cgccaattat
    11521 cccgcgcgta aatttggcgt acgtagcgct atgcctactg ggatggcgct caaattatgc
    11581 ccacatctca ccttgcttcc ggggcgcttt gacgcctaca aagaagcctc aaatcatatc
    11641 cgtgaaatct tctcgcgcta cacctcgcgc attgaaccgt tgtcactgga tgaggcttat
    11701 ctcgatgtca ccgatagcgt ccattgccac ggttctgcga ccctcatcgc ccaggaaatc
    11761 cgccagacga tcttcaacga gctgcaactg acggcatctg cgggcgtggc accagtaaag
    11821 tttctcgcca aaatcgcctc cgacatgaat aaacccaacg gtcagtttgt gattacgccg
    11881 gcagaagttc cggcattttt acaaacctta ccactggaaa aaatccccgg cgtcggcaaa
    11941 gtctcggcgg caaaactgga agcgatgggg ctgcggacct gcggtgatgt acaaaagtgt
    12001 gatctggtga ttctgcttaa acgctttggc aaatttggcc gcattttgtg ggagcgtagt
    12061 caggggattg acgagcgcga cgttaacagc gaacggttac gaaaatccgt cggcgtggaa
    12121 cgcacgatgg cggaagatat tcatcactgg tctgaatgtg aagcgattat cgagcggctg
    12181 tatccggaac ttgaacgccg tctggcaaag gtaaaacctg atttactgat tgctcgccag
    12241 ggggtgaaat taaagttcga cgattttcag cagaccaccc aggagcacgt ctggccgcgg
    12301 ctgaataaag ctgacttaat cgccaccgcg cgtaaaacct gggatgaacg ccgcggcggg
    12361 cgcggtgtgc gtctggtggg gctgcatgtg acgttgcttg acccgcaaat ggaaagacaa
    12421 ctggtgctgg gattatgatg tatactatta tgtatattct ggtgtgcatt attatgaggg
    12481 tatcactgta tgcatcggat tctcgctgaa aaatcggtca atatcactga gttacgtaaa
    12541 aacccagcta aatactttat tgatcaacca gttgcggttc tttctaataa tcgccccgca
    12601 ggatatctct taagtgccag cgcattcgaa gcgttaatgg acatgcttgc tgaacaagag
    12661 gagaaaaagc ccataaaggc gcgctttcgt ccaagtgctg caagattaga ggaaattaca
    12721 cgccgcgcta aacaatatct taatgatatg acggatgatg atttcaatga ctttaaggaa
    12781 taaggatgcg ggtattcaaa acaaaactta ttcgcctgca acttacagca gaggaacttg
    12841 atgcgttaac ggcggatttt atttcctata agcgtgacgg tgttttgcca gatatatttg
    12901 gtcgcgatgc actctacgac gactcgttta cctggccatt aatcaaattt gagcgagttg
    12961 ctcatattca tctggcaaat gtgaataatc catttccgcc acagttgcgc caattcagca
    13021 gaacgaatga cgaagcgcat ttggtatatt gtcagggtgc gtttgatgag caagcatggt
    13081 tgctcattgc cattctgaag cctgaacctc ataaactggc tcgagataac aaccaaatgc
    13141 ataaaattgg aaaaatggca gaagcgtttc gcat
//
LOCUS       DADLZM010000111        12881 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLZM010000111 DADLZM010000000
VERSION     DADLZM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.112"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(511..1602)
                     /gene="ychF"
                     /locus_tag="JNY73_003041"
     CDS             complement(511..1602)
                     /gene="ychF"
                     /locus_tag="JNY73_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY8048968.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1719..2303)
                     /gene="pth"
                     /locus_tag="JNY73_003042"
     CDS             complement(1719..2303)
                     /gene="pth"
                     /locus_tag="JNY73_003042"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY8048969.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2581..2859
                     /gene="ychH"
                     /locus_tag="JNY73_003043"
     CDS             2581..2859
                     /gene="ychH"
                     /locus_tag="JNY73_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY8048970.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="JNY73_003044"
     CDS             complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="JNY73_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY8048971.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4718..5665)
                     /locus_tag="JNY73_003045"
     CDS             complement(4718..5665)
                     /locus_tag="JNY73_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY8048972.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="JNY73_003046"
     CDS             complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="JNY73_003046"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY8048973.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="JNY73_003047"
     CDS             complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="JNY73_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY8048974.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7504..8760
                     /gene="hemA"
                     /locus_tag="JNY73_003048"
     CDS             7504..8760
                     /gene="hemA"
                     /locus_tag="JNY73_003048"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY8048975.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8802..9884
                     /gene="prfA"
                     /locus_tag="JNY73_003049"
     CDS             8802..9884
                     /gene="prfA"
                     /locus_tag="JNY73_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY8048976.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9884..10717
                     /gene="prmC"
                     /locus_tag="JNY73_003050"
     CDS             9884..10717
                     /gene="prmC"
                     /locus_tag="JNY73_003050"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY8048977.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10714..11106
                     /locus_tag="JNY73_003051"
     CDS             10714..11106
                     /locus_tag="JNY73_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY8048978.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11110..11919
                     /locus_tag="JNY73_003052"
     CDS             11110..11919
                     /locus_tag="JNY73_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY8048979.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEEPISEMIRAQINNIAHKHIVLH"
     gene            11955..12809
                     /gene="kdsA"
                     /locus_tag="JNY73_003053"
     CDS             11955..12809
                     /gene="kdsA"
                     /locus_tag="JNY73_003053"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY8048980.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3228 a         3232 c         3330 g         3091 t
ORIGIN      
        1 attttcgctt gatgtatctg cataacaaaa acaatatgcg ccatttttgc attactaata
       61 agaaaaagca aaaaacatcg atattcattt ttggtcaatg gattttctgg cctatcgtta
      121 ttattgtgct actggttatc attttcatct tgccaggatc gcacgtaatc acgttattcg
      181 gggactttat ctgcgggata ttacccttca ggtaagtcct tttctcgcca gcatattgga
      241 gcgagatcaa tattgatcag ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg
      301 ttatatcact gctaatgaga tatcagtcat ctgaacactt atgtctttaa ttattctcgt
      361 ggttcactgt aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg
      421 tgaaatgaaa aatccacgca attgcgtgga ttttatatac ttttgcgctc ttcatgagat
      481 ttagcgaaac ctcatgagac aataaattaa ttagacgttg aaaaggaagt tcatcacatc
      541 gccatctttc acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc
      601 ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc
      661 tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat
      721 ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt
      781 ataaccggca cggatcacac ggttcaggcc cggttcttcc agcccaagct cctgcataaa
      841 ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg
      901 aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt
      961 ttcaaaaccg tcttcgttga cgttggctat gtacattgtt ggtttcagcg tcaggaagct
     1021 caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc
     1081 gttttccagc tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc
     1141 gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc
     1201 cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac
     1261 gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg
     1321 gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc
     1381 ggcgatatcg acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc
     1441 cagttgatcc aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca
     1501 gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt
     1561 cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat
     1621 atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat
     1681 tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc
     1741 aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct
     1801 tcgtcgatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa
     1861 ccgacaactt tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta
     1921 gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg
     1981 cccaacttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt
     2041 tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc
     2101 ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagggtgac tcgcgaagta
     2161 taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca
     2221 acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg
     2281 ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta
     2341 gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata
     2401 attgcctgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt
     2461 tgaaataagg gttgtaattg tgattacgcc cgcacataac ccacagggtg ttgtctatac
     2521 tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat
     2581 atgaaacgca aaaacgcttc gatactcggt aacgtgctca tggggttggg tctggtggta
     2641 atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag
     2701 tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt
     2761 gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg tcactatgac
     2821 aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac
     2881 cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg
     2941 attcgggaag aacgcgaggc gtcccgggat cggttgaatg cctgcgcgag ccatagttcg
     3001 cagtggctgg aattccacgt tgcacacgcg cagttcacat ccttcgggca gacgcttcac
     3061 aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaacggcat cccacttcag
     3121 aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaataagc cttcagcagc
     3181 agcaaaaaac agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac
     3241 taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac
     3301 gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcagtgaca tacacagcag
     3361 catgacgatg atgtcatctt tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc
     3421 actcatgttc cacgccacca tcaacagcag ggctgccatg gcggaaagcg gcagccagga
     3481 gagcagtggt gccagtacca gcagggcgag aataaccaga atagagtgga tcaccgccga
     3541 gataggggaa gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc
     3601 ggtaatacca ccaaagaatg gagcgatgat attccccagc ccctgaccga ccagttcact
     3661 attcgccttg tgcttagtcc cggtcatacc atccagtacc acagcgcaga gcagagattc
     3721 gatcgccccg agcattgcca ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca
     3781 ggttagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg gcagcagttg
     3841 cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt
     3901 agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc
     3961 cggaaggtga ccgggtaaac gaatgcccag acgcggccag aaaaccagaa tgccaagcgt
     4021 cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc
     4081 gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat
     4141 ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg
     4201 aatatactca atcagacgac caaagcgtgc cagccccatc agaatcaaaa agattcccga
     4261 caacaaggtc gcaaccagca gtcctgccag tccaaactgt tgcgaaacgg gatagagaat
     4321 taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gacccacccg ttagagcaat
     4381 gacaatcccc gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat
     4441 agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc
     4501 acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa
     4561 aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca
     4621 tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa
     4681 aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga
     4741 tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag
     4801 tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga
     4861 cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag
     4921 tcgcgtacgc aaatacacgt ttagcaccac gttctttcag cgcttcagca gctttacaca
     4981 gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac
     5041 cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca
     5101 tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg
     5161 gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc
     5221 taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat
     5281 ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag
     5341 tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga
     5401 taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt
     5461 ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc
     5521 gtacattttc gttaatttgt acgctgactt cgccgtcgct aaagcgacct acagcggcgt
     5581 cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagtaccggg gtggcgttac
     5641 cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa
     5701 gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc
     5761 gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa
     5821 gcatggctct gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca ttgagccatt
     5881 ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac
     5941 aggcccctgt cccagtcagg cgcgacgggg cgtattttaa cagccaggaa agcaccgcat
     6001 caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta
     6061 gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca
     6121 ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca
     6181 ccggcgttag tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag
     6241 gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt
     6301 gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac
     6361 cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac
     6421 gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt
     6481 catgttccac gccttcaacg ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa
     6541 tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat
     6601 ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg
     6661 tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt
     6721 gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac
     6781 cttccagttt ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag
     6841 tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg
     6901 aattggcatt ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg
     6961 acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct
     7021 gcccaatggg ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa
     7081 gcgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg
     7141 attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc
     7201 aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag
     7261 gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca
     7321 gttataaccc ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat
     7381 tgccagaatc taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc
     7441 ttacgcatcc tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca
     7501 gacatgaccc ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa
     7561 cgtgtatcgt tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg
     7621 atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt
     7681 gaagagcagg ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt
     7741 aatgaagaag atctgcgtaa aaacctctac tggcatcagg ataacgacgc ggttagccat
     7801 ttaatgcgtg ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt
     7861 caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag cgaactggaa
     7921 cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac agatatcggt
     7981 gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg
     8041 ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt
     8101 catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc
     8161 caaattctgg cagatgaagt tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt
     8221 ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa
     8281 ggcatggtgg agcgcgcatt aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt
     8341 gccgttccgc gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagt
     8401 gtggacgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt
     8461 gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa
     8521 agcgccagcg aaaccattcg cgagtatcgc agccaggcag agcacgttcg cgatgagtta
     8581 accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat
     8641 ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc
     8701 gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag
     8761 cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg
     8821 ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc
     8881 aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg
     8941 atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga
     9001 tgatgctcga cgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag
     9061 aaaaaagcga gcaactggaa cagcaattac aggttctatt actaccaaaa gatcctgatg
     9121 acgaacgtaa cgccttcctc gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt
     9181 ttgcaggcga tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag
     9241 aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta
     9301 gcggtgatgg tgtgtatggt cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg
     9361 ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc
     9421 cagaactgcc tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt
     9481 tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta
     9541 ctcacttgcc gaccgggatt gttgttgaat gtcaggacga acgttcacaa cataaaaaca
     9601 aagcgaaggc gctttcagtg ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc
     9661 aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta
     9721 accgtactta caatttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct
     9781 accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg
     9841 aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg
     9901 gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat
     9961 cctgctggag catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca
    10021 gctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga
    10081 acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttctcc
    10141 agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt
    10201 gcctgaacaa ccttgccgta ttctcgatct cgggacgggt accggggcga ttgcgcttgc
    10261 gctggctagc gagcgcccgg actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt
    10321 ctccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag
    10381 cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta
    10441 tattgacgag caggacccac atcttcaaca aggcgatgtc cgctttgagc cgctcactgc
    10501 gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa
    10561 cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt
    10621 gcgacaagca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga
    10681 taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt
    10741 gttcatctta ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt
    10801 tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat
    10861 acggtgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttcc
    10921 gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt
    10981 tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg
    11041 ctggcgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg
    11101 gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc
    11161 atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa
    11221 ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag
    11281 cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc
    11341 gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt
    11401 gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg
    11461 ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg
    11521 ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag
    11581 ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt
    11641 gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg
    11701 gagctggcgt tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa
    11761 attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat
    11821 ttaagttatt tcgttgaaca gtgtccggaa gagccgatca gcgaaatgat ccgtgcgcaa
    11881 ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag
    11941 attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg
    12001 acctgccgtt cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc
    12061 gcatttgcga gcactacgta actgtgaccc agaaactggg tatcccttac gtgttcaaag
    12121 cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag
    12181 aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg
    12241 ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg
    12301 cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta
    12361 acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca
    12421 aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca
    12481 acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg
    12541 tgattttcga cgtgacccac gcactgcaat gccgcgatcc gtttggcgca gcttccggtg
    12601 gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc
    12661 tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc
    12721 cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag
    12781 gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg
    12841 ttctgcgtta agactttttt catgggtgcc agatacaaaa a
//
LOCUS       DADLZM010000112        12862 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLZM010000112 DADLZM010000000
VERSION     DADLZM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.113"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            77..865
                     /gene="modE"
                     /locus_tag="JNY73_003054"
     CDS             77..865
                     /gene="modE"
                     /locus_tag="JNY73_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY8048981.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNAATSLQQGQNVTAYFNADSVIIATLC"
     gene            933..2405
                     /gene="modF"
                     /locus_tag="JNY73_003055"
     CDS             933..2405
                     /gene="modF"
                     /locus_tag="JNY73_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY8048982.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            2666..3682
                     /gene="galE"
                     /locus_tag="JNY73_003056"
     CDS             2666..3682
                     /gene="galE"
                     /locus_tag="JNY73_003056"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY8048983.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            3692..4738
                     /gene="galT"
                     /locus_tag="JNY73_003057"
     CDS             3692..4738
                     /gene="galT"
                     /locus_tag="JNY73_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY8048984.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            4742..5890
                     /gene="galK"
                     /locus_tag="JNY73_003058"
     CDS             4742..5890
                     /gene="galK"
                     /locus_tag="JNY73_003058"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY8048985.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            5884..6924
                     /gene="galM"
                     /locus_tag="JNY73_003059"
     CDS             5884..6924
                     /gene="galM"
                     /locus_tag="JNY73_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY8048986.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            7127..7879
                     /gene="gpmA"
                     /locus_tag="JNY73_003060"
     CDS             7127..7879
                     /gene="gpmA"
                     /locus_tag="JNY73_003060"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY8048987.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="JNY73_003061"
     CDS             complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="JNY73_003061"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY8048988.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAIRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            9414..9794
                     /locus_tag="JNY73_003062"
     CDS             9414..9794
                     /locus_tag="JNY73_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048989.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            9908..10849
                     /gene="zitB"
                     /locus_tag="JNY73_003063"
     CDS             9908..10849
                     /gene="zitB"
                     /locus_tag="JNY73_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY8048990.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(10846..11565)
                     /locus_tag="JNY73_003064"
     CDS             complement(10846..11565)
                     /locus_tag="JNY73_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY8048991.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="JNY73_003065"
     CDS             complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="JNY73_003065"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY8048992.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
BASE COUNT         3206 a         3355 c         3239 g         3062 t
ORIGIN      
        1 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
       61 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
      121 attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag
      181 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
      241 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg
      301 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
      361 ccagcaaaaa gcctttgatg tgttaagtga tgatgacgcc ctgccgctga acagcctgct
      421 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat
      481 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
      541 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg
      601 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt
      661 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
      721 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
      781 aaatgcagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
      841 cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
      901 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
      961 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     1021 atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg
     1081 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     1141 tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat aacaccgata
     1201 tgctcggccc tagcgaagat gacaccggac gcactacggc tgagattatt caggatgaag
     1261 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     1321 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     1381 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     1441 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actttggtac
     1501 tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc gtgctggcgg
     1561 attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc
     1621 aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     1681 cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc gtggtttctt
     1741 ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc gaacactggc
     1801 aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt actggcgatc
     1861 atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa
     1921 ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     1981 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     2041 atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     2101 ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     2161 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     2221 aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg ctgattagcg
     2281 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     2341 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     2401 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt
     2461 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     2521 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     2581 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     2641 taccataagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     2701 tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     2761 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     2821 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca
     2881 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa
     2941 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     3001 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     3061 ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag
     3121 caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat
     3181 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     3241 tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg taggccgtcg
     3301 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     3361 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     3421 caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt
     3481 ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg
     3541 cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     3601 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     3661 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc
     3721 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     3781 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     3841 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     3901 cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     3961 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     4021 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     4081 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     4141 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
     4201 ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt
     4261 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
     4321 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
     4381 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
     4441 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
     4501 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
     4561 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
     4621 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     4681 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     4741 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     4801 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     4861 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga
     4921 tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     4981 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
     5041 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
     5101 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     5161 attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     5221 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
     5281 cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc
     5341 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
     5401 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
     5461 agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
     5521 cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
     5581 cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
     5641 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
     5701 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
     5761 cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
     5821 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
     5881 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
     5941 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttcc
     6001 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg
     6061 gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta taccaaccgc
     6121 atcgccaata gtcgttatac ctttgacggt gaaaccgtga cgctttcgcc aagccagggc
     6181 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
     6241 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
     6301 ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
     6361 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
     6421 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
     6481 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
     6541 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
     6601 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
     6661 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
     6721 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
     6781 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
     6841 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
     6901 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
     6961 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa taaccttaca
     7021 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
     7081 ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg ctgtaactaa
     7141 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
     7201 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
     7261 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
     7321 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
     7381 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga
     7441 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga
     7501 actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga
     7561 gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
     7621 gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg
     7681 taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga
     7741 gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
     7801 gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa
     7861 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga
     7921 gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc
     7981 gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
     8041 aaaccttacc cgcgacgcgc ttttactgca ttcgccagtt gacgtaacag agcatcggta
     8101 tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta
     8161 tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt
     8221 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
     8281 ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc tgctttgttc
     8341 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
     8401 agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg cccccattta
     8461 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
     8521 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
     8581 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
     8641 agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta
     8701 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
     8761 atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag
     8821 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgaatg
     8881 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
     8941 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgagc aaccgtattc
     9001 gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag taactctttg
     9061 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gtcgccatac
     9121 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
     9181 tttacactat gaacggatta atcctagaat tagcaagaaa aacgccagat tgtcgcgaaa
     9241 aacgagatct ttcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
     9301 tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca
     9361 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
     9421 tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac
     9481 tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg
     9541 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
     9601 taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg
     9661 gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata
     9721 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
     9781 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
     9841 atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
     9901 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
     9961 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg
    10021 tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac
    10081 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    10141 ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct
    10201 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    10261 ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    10321 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    10381 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    10441 ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    10501 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt
    10561 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    10621 tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    10681 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    10741 tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga
    10801 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    10861 gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa
    10921 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    10981 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag
    11041 aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg
    11101 ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg
    11161 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    11221 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc
    11281 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    11341 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    11401 aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    11461 cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcccgcccc
    11521 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    11581 taacgttacg ccttattatt cgttatccac gtagtgtaac cgcaaaatcc agcatacgat
    11641 tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc
    11701 cttcctgttc taatgcctct gcgatagcct gaaggccatt catggccatc cacggacaat
    11761 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    11821 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct
    11881 gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    11941 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    12001 cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc
    12061 agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg
    12121 cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg
    12181 aagttaccac ccaatctgcg cgcgttttta ccgcagcaga agtgttggcg tagacgacga
    12241 cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat
    12301 ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    12361 tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg
    12421 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    12481 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    12541 gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    12601 gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt
    12661 ttacaggctt aacgttaaaa ccgacattag cgtaacattc gcttttatcg gtgtaatttg
    12721 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat
    12781 tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt
    12841 gaaattgagg atgcctgaat ag
//
LOCUS       DADLZM010000113        12783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLZM010000113 DADLZM010000000
VERSION     DADLZM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.114"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(67..2016)
                     /locus_tag="JNY73_003066"
     CDS             complement(67..2016)
                     /locus_tag="JNY73_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8048993.1"
                     /translation="MTDSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2101..2437)
                     /locus_tag="JNY73_003067"
                     /pseudo
     CDS             complement(2101..2437)
                     /locus_tag="JNY73_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2437..2793)
                     /locus_tag="JNY73_003068"
     CDS             complement(2437..2793)
                     /locus_tag="JNY73_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8048994.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2793..4289)
                     /locus_tag="JNY73_003069"
     CDS             complement(2793..4289)
                     /locus_tag="JNY73_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8048995.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4286..4447)
                     /locus_tag="JNY73_003070"
     CDS             complement(4286..4447)
                     /locus_tag="JNY73_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY8048996.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4457..5019)
                     /locus_tag="JNY73_003071"
                     /pseudo
     CDS             complement(4457..5019)
                     /locus_tag="JNY73_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5016..5540)
                     /locus_tag="JNY73_003072"
     CDS             complement(5016..5540)
                     /locus_tag="JNY73_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8048997.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRYARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5506..5922)
                     /locus_tag="JNY73_003073"
     CDS             complement(5506..5922)
                     /locus_tag="JNY73_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8048998.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5919..6242)
                     /locus_tag="JNY73_003074"
     CDS             complement(5919..6242)
                     /locus_tag="JNY73_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8048999.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6287..8017)
                     /locus_tag="JNY73_003075"
     CDS             complement(6287..8017)
                     /locus_tag="JNY73_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8049000.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGTTSGWVG
                     ETEARQETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7995..9236)
                     /locus_tag="JNY73_003076"
     CDS             complement(7995..9236)
                     /locus_tag="JNY73_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8049001.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9236..9418)
                     /locus_tag="JNY73_003077"
     CDS             complement(9236..9418)
                     /locus_tag="JNY73_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049002.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9430..11187)
                     /locus_tag="JNY73_003078"
     CDS             complement(9430..11187)
                     /locus_tag="JNY73_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY8049003.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEEWGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11187..11669)
                     /locus_tag="JNY73_003079"
     CDS             complement(11187..11669)
                     /locus_tag="JNY73_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY8049004.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11817..12167)
                     /locus_tag="JNY73_003080"
     CDS             complement(11817..12167)
                     /locus_tag="JNY73_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8049005.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12210..12674)
                     /locus_tag="JNY73_003081"
     CDS             complement(12210..12674)
                     /locus_tag="JNY73_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY8049006.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(12671..>12783)
                     /locus_tag="JNY73_003082"
     CDS             complement(12671..>12783)
                     /locus_tag="JNY73_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8049007.1"
                     /translation="GACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT         2883 a         3793 c         2960 g         3147 t
ORIGIN      
        1 aaacaaactc cattgttagc atgttacgcg ataattttgc ctcatctttc accacaaaaa
       61 aagaactcac cttttaaatg gagaataccc aacatcatgg gtgattttca taaagggatc
      121 gcctgttttc ggcaaatcga taacgcgcat tccaggcggc gcgttgtcaa acgtcacctt
      181 aagttcgctg cgggttgccg gtgaagatgc aaggcttaac agctgattcc tttccagtga
      241 tacattcggg gtgtagcgtt tatcgggaga taaataccca tcaccacgtg gcatctgcca
      301 tccggtcatg tcatagacaa aatcatggaa ctgtttgccc cactcgtcga acttatcatt
      361 cagaccaaat ccactgttca gtgcatcagc cacaaaattt ttaacaagcc agggatgttc
      421 tttttcaaac tcctgcgcca tcatgcccag ttcaagcaat ccaccaatca gactgatctt
      481 tccgaggccc tttaatttca gccctgtttt tcctgccgcc gttgtccatt gattctgggc
      541 gactgttgcc gcacgaatac ttgccaccgc cttcacacca atatacaggg cagaaaccgt
      601 tgctattgtt tttactgcct gttcccatcc ccccattgct ctgacaacct gatcaacctc
      661 ctgccagact tttttcacaa ccggaccgac cgtttcccag ttatcaatta tgagatatgc
      721 accaccgacg agaagcgcaa ttaatccctt tgccggcgtc atattcatca cgctacccat
      781 gatttttgtt atgcgtgtca atgtgccaat cgccacgccc atcgtcagta atgccgcacc
      841 agttttcgcg atggttttta cgacctcagg attttctctg acaaacgtgc gcacttcttc
      901 cagaaaaggc ttcatctctt ttatgccttc attgagtgaa ggcaggaagg tttcccccag
      961 cgtggaagaa atcgcattga tctggttctg cagcagcaac aactgatttt ctgtcgtcgc
     1021 ggcacgggcg gcatattctt tctgcattga gccgccatac tgctgcgcat ccgccacacg
     1081 attaaagttg gttcgcaaca aatccagatt cgtgagcagc ggcgcaatgg cccccaggga
     1141 ttccttcccg aacagcgctt tcagcacagc agcctgtttt tctttcggca cttttgccag
     1201 agaatccagt acgtgcagca tggccccgcg ggcgtctttc tgcatatccg ccgccagttt
     1261 tttcgggcta atgcgcaacg agcggagcac ttttttctgc gatttggtgg cagaatccct
     1321 cgctgtcagc gacaacataa aattctttat ccccgtcgca gcaatttctg actccacccc
     1381 cattccggca atggttgccc ccattgcggc aatctctccg gaggccacac ccgcaacact
     1441 gcctaaaggg ccaatacggg tcacaacatc agaaatcttt ttcgcacttg caggaccggt
     1501 attgccaaga tagttaatct tatccgcaag ccctgccacc tctccctgtg tcagtttaaa
     1561 ggcagtgcgc cactgcgcca tcatctgacc ggattcttct gccgtggcgt caaaggccac
     1621 gcccatcttc acggcatcgt cagtaaactg catcagctcg tcacgggcga taccagcctg
     1681 accacccgcc gccacaattt cggcgatacc ttccgccgac atgggcagtt ctgttgacag
     1741 gtcgcgcacc tgctccgtca ttgccttaaa cgcttccggc gtatccagac catccaccac
     1801 tttccggaca tccgccattt ttgattcaag ggcaatggca gatttgaccg ggagcgccag
     1861 cgcccccagt actgcggttc cggcacccgc tgcgcccagt gacaggctgg caaactcctt
     1921 tttaaaacct ttcagctggc gctgcattcc cttcagcggt gccgatacct tatccacggc
     1981 agtgatgatc gccttcagct gaaaactgtc agtcatgctt catctcctcg ttaatgcgga
     2041 cggcctcggc ttccagttct gtaaactgcg aaacagccac ccgtcgcagc tccagtggat
     2101 tcagtttcca gaaccaggca acattgtaga gtcgtttccc ggaggtgctt cccgtctccg
     2161 actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa aatatccagt tttgccatct
     2221 gcgccgccga tgaacgcggg atccccgcca gcaacgggat atatttcagt gccacccggc
     2281 tgtccagttt aatactgcct tcaccggaaa taatgaaagg aaaacccagc gcctcgattt
     2341 cgtcatacgt gggctcgcgc aactccagca catgcagcat ttcgttatgc gctgtcaccg
     2401 gtttttttaa aactatttct gtaacgttca ttgccattac tgataccctc cctcttcgcc
     2461 gtggaattca agatctgccg tgccttcttc ggcattatga ttagcctctc cgtgcagcca
     2521 tgccgccgaa agcacataca ccatgccgtt tgccagctca gcggtaatcg tcatctggtc
     2581 tgaggtggta attttgtcga ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta
     2641 aggtgaccgc caggtctcct tgtaatccac atcccctgcc atgccgacga tgtcatcccg
     2701 gacattggtg ttcatcggca cttcaatgcc acctgtcagc gataactgct gaccatccac
     2761 tttaaaaaaa caggttccgg cgatctttgc cattatgctg actcctctga atactgaaga
     2821 cggaactggt taaccaccgc aaagacacgt aactgattaa cataatccgg cgggaacagc
     2881 gtattcagtc ggttcgggtt atctgcatca cgctcaacta tcagatactg tttaaacaga
     2941 tcgtaatttt ccacaatacc ggcacgctcc atctgacgat atgtcgccag aagttcccct
     3001 ttgataacgg caggagtgac aatcgcctgc cccggaccaa aacgggtacc atcatttgcc
     3061 agcttgtgac gtccgtactt gcttgtgatg accgatttca gtttgcgaag aacatatgca
     3121 ctggtatgca gagtttcact gtccagatag ctgttgtctg ccacgccata cgcattcttt
     3181 ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc cttccacata agccgtcgcc
     3241 acgccgtgag ataaaagggt ctgctgctct gtcatgatga atcgcttacc tttcggtgcc
     3301 ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg caggatcatt ccggataaat
     3361 accgcttcac gggcaaggcg actggcaacc agttcatcga caggcgactg ggtttctttt
     3421 tcgtaaccgg caagcgtgat atgttgctga ttatgcatat ctccggcacc aaccagctct
     3481 gacagcgttc ccagttttgc ggtatagaca tgcccgtata actggcgcgc ataactccag
     3541 cgaccgctgc tgtcattcat ttcggtcatc atcatattga tggaggcggc atcgttgaac
     3601 ggcagaccga taaagtcgaa tgcctcatcg cccatagcag caacagcggc ggtaagatca
     3661 ggcgctccgc taccggcagc accgacttcc gtcaccaccc gaagccctgc tggcagaatc
     3721 tcaccaccac cagaaccata ataattcaga cagaccggta actcattacc atacagcccc
     3781 ttatgacggg cagtcagcgt caccacgcct gcatcagatg acgccgtaaa cggcagggtg
     3841 ataaccccgt ttaccgcttc cttaatcgcg gtggcaaccg cagtggcatc atcgccattc
     3901 accacaggca cctgtacacg ggagcgcccg acatacagac tcagggtgcc gctttcctct
     3961 gcttctccgg caaccgtcac cctgaccgtc gccgccgccc ctctggcttc cggtactgca
     4021 ataacataca gttcaccgaa aggatctgtc tgacggtaga cttccaccat acgcgccagc
     4081 tggctccccg ccccacaaat ctggcgggca taatctgccg atggcatcag caccaggctg
     4141 ttaacctcaa tggctgcatc gttgctggca tgcccgatca gtaatgcagg cgcgctggtc
     4201 accgctgtat ttgccgcaga attgttcatc tcggcgtaga acaacggcac cagcgtattc
     4261 gacggaacag tattaaaact tatcgtcatg attttttagc cttatcctgt ttaacacgta
     4321 ccacatcacc cgccgctata cggcggaacc agtagctgct ttcttccaca tttcgccctt
     4381 cggaaggcaa aaggtctcca cgggcagggt caggaaccga ccgccctttc aggggtttca
     4441 caaacatgag ggactcttaa ttctgaggga aacgcatttc cagatggtgc tcaatgtcac
     4501 catccgggcc agtaccagga tcgatgtaat ccacatcaat actcagcagg gataaatccg
     4561 gcaggcattc acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt
     4621 aaaatcaaac tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata
     4681 aacaatctcg cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct
     4741 ccggacatca tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct
     4801 gagcaccaca atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt
     4861 ttgttccccc ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc
     4921 ggcatcaggg atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca
     4981 tcgctcacgc agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga
     5041 cgtaacgaac gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca
     5101 ataacgtcag ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc
     5161 ttctgccgct tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga
     5221 aacgccggat aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg
     5281 tcaggggaaa ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa
     5341 ccaatggagc gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt
     5401 ccgcgtttaa tcaccagacg tctggcataa cgcatgtaaa cgcgcccgat ttgcacaaac
     5461 gcccgacgca gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt
     5521 aaaaatgctg tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca
     5581 ttccagtaat aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc
     5641 ttggccgtaa taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa
     5701 ataatgcgta acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg
     5761 agccaccttt gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc
     5821 ggtgggttca tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg
     5881 taaaaggtaa gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa
     5941 gggccagcaa gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt
     6001 gagcgatttt cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc
     6061 agatgcatcc cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg
     6121 ttcaggtatg tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg
     6181 tcactgtaca aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc
     6241 atattttcct cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt
     6301 tctggcgggt tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac
     6361 cgccggttcg cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca
     6421 ggatacgggt accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa
     6481 tggcttttgc atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg
     6541 ccagagaaga aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat
     6601 ccttcagcag acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc
     6661 ggtgcgcctt acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag
     6721 ccgcgccgga agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg
     6781 tggactcata agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa
     6841 tttcttcctg ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa
     6901 agaaagcatc atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa
     6961 agggctcaat cagtcccagt tttgaggtgg cggtttcctg acgcgcttcc gtttccccca
     7021 cccatccgga cgtcgtgccg cccagattca ccagtttttt ataatccgag ccacccagag
     7081 tgattacggt ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc
     7141 gatccagttc ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct
     7201 ttcgctcaag ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt
     7261 ctttatgttc cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca
     7321 gctcagcctc aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt
     7381 ccacttctcc gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat
     7441 agcccccggc catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc
     7501 tttcgatttt tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga
     7561 tccgcgcctc gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt
     7621 tcagcagaaa ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag
     7681 aaaggccggt taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat
     7741 catcaataag caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt
     7801 aaacaccgat gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt
     7861 cactccacgc ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt
     7921 cctttacacc aaagacggag ccatgccctt caaactcacc ggagtcactg acagatttca
     7981 gactcagcgg tacatcaaga cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg
     8041 gctttactgc catcggaggg tttcgtggtc atgttcatcg gtgtgagata gacatcccca
     8101 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tgggagagta aatcccccag
     8161 ttaatcccgg tggcgtaggc ttcaaaacgg gatttcatat ccccgcgcag taacgccccg
     8221 gcgttaaatt tggcgtaaaa cttcccctgt ttgctctccc tgaccagccc tgtattgatc
     8281 cgttgttcaa tacgggtcag atacggcaca agggaatagt tgataaatcc gagccccagc
     8341 tcttcaatat tgttgaaagt ggcgcgatcg gtgttctgca ccatgtgcag cggcacgcgg
     8401 aaaagacgac agatttcttc caactgaaac ttgcgggttt ccaggaactg gctgtcctcg
     8461 gcattcagtg ccaccggctt ccagtccagc cccatttcca gaatcatcgg acggtgcgca
     8521 ttacccagcc caagatgacg ctcctcaaaa tccctcttca tgcgctcata agcatccggc
     8581 gtgagctttt gttccgtgcg caacacaccg gatgtcaccg caccattacc aaacaacctg
     8641 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cgcgcgcata tgcaatggga
     8701 ttcagcccga ccagtccgtc cagcgtcagg gtgcgcacat gccagatttc atcctgggtc
     8761 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
     8821 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccacaacttc ccccagcgcc
     8881 ttcaccttgt aagcgtaaaa attcccccgc agacacaggc agacgataac cagctcccag
     8941 aattcctgcg gcgtcatgta gccattgggt tttgctgaaa tcagcttatg cagccgttca
     9001 tccaccgccc gtgttttaag ggtgccgctg attttgtaga gactgcaggg cagcatacca
     9061 acagactcag ccagcaccct gacgcaggaa tagaccgccg tcagccgcat ggcccgctgg
     9121 ctgctgatcc gctttccggt ataggtgtcg tatgacaacc ccagctcttc cgcaagcatc
     9181 ccgggcgttg tgacgggggt gttatttttg cgttgaaaaa gcccctggaa aaacattact
     9241 cacctccgga ggcgacccgg tgaccgcgat cgagataacg cgcaaccagc cacgaccagc
     9301 acagacacag cgccccggca acaacaaaac ctgccggggg ataaatcagc catgtgccat
     9361 acgacagcaa aagcgccccc agcacgccca ccagtggcgt gagaattatc agaaacataa
     9421 tgacctcggt taaagcgagc gaataccaac gctgaccaga tgctcagaca gatccggctc
     9481 cggttcaccg ccattgacca gcatccggct cattgctgta aacatcgcaa cagggccgtc
     9541 gattttggct tccggcgtgg atttgttcgg gaagatattg tcgtttttgt ccggtttcac
     9601 cgtaacgtta gacatcatcc agttcatgac cgggtgatta ctgtgatgga aacgcccggc
     9661 atagaccagt gattccgttt ccttcatggc ctctgacaga ttgcggaccg tctgcggaac
     9721 ttccaccagc ggtatacctt cttcagccag cgccagactg aactgcattg cgctccacgg
     9781 gtcaaatccc agttccctca ggttttcacc gccaatccat tccagtaagt cactttttat
     9841 ctgagcatga tcgataacat caccatccgt cagaatcagc ttatccatct ccgcccactt
     9901 ccggtaaagt tctgcctgct gccgcgagca tcgttccagc cgtccttccg gaagccagaa
     9961 tttaaaatcg gcatgaacat gcccgttatc cgttcgccag agttttgccg ccgcacagat
    10021 atcaatctta tgagcaaggt caacgccgac ccacatggga tacgttttca gctcatgtcg
    10081 tggggcaatg tattcgcact tctcccactt aatcatgtcc atccaggcag actctgctgt
    10141 tacccacaca ttcatgtgtt tggtaaaaaa attcacccgc gcagagacct gttctttcgc
    10201 ttttttcgcc aggcgacgca gatcatccca gcgtttacag atgcccaggc cgggattcgc
    10261 tttctgccag accgtttcat caaacggatc atctccctca tcgagggtgt aaataatcgc
    10321 aaagtaggag tcgtctttta ccgcgccctc cacatcgctg ttatagccac gcaatacctt
    10381 gatggcgtaa tcacgctgct cgtaacaaat cccttccttg ttaaaccctg ccgtggtgat
    10441 accaaataaa agggactgca gacgggcacc ggttgccgtt tccagaacgt cccacacgtc
    10501 acgggtttta tgtgcatgca gctcatcaat aatggcgcag tggatgttca gaccatccag
    10561 gttgtttgca tctgaagaaa gcggttcaaa ttttgatgcg ctctgctcct gataaatcgc
    10621 cagcttgttg aaatcaaaca accgcccgag tgtcgaccgg gcttttctga ccatattttt
    10681 ggcgtcttca aacacgattc tggcctggtc acgcgtggtt gcggctgaat acacctcagc
    10741 cccgccttca ccatctgccc ccgtcatata cagaccgata cctgatgaca gggttgattt
    10801 tgcgttttta cgggcgactt cgttgtacgc tgtccggaaa cggcgcacca tcaccgggcg
    10861 tccgctgcca tcgctgcgca tgacaacttc cccggtctct tcattcacca gcggaatgac
    10921 aaaaccaaaa atattaatga ggataaacac atgccagtcc atcaactcaa tgggctgacc
    10981 tgccagcgcc ccttttacat ggggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg
    11041 gggttcactg aaataaatcc cccactcttc gccgtacttc agatcatcaa gaaaacgctg
    11101 gcaggccaga cggacaaatt caccagcgac aatttctcct gcaacaacac gttcggcgta
    11161 gcggatcccg tcagccactt ttgccatcag tctctcgctt ttaaaagttc tgccagcgga
    11221 tcaacatcat ccggtccggc gatatttact ttcgcccggc ttgccggtga cataccaaat
    11281 tctgcaagca tcgcccggat ccgcttccag gcatccgctt tcatcgcagc agccgggtgt
    11341 gccttaatca gcacatcacc gttctgcgtt tccgtgcggt aggtataacc ctcaacatcg
    11401 agggtttcgc agtgatgccg gtattcggtg taggcttcca ccagcaactc gagtgcacgc
    11461 gcatcaagct gagaaatgat cccttccgca ttcagctctt ccgccattcg cctgaaccag
    11521 tactttccct gcgcccctaa atgctgcgga attttaggga gacctttttc gtccttttta
    11581 gcggtttttt tggcgtcttt aacgggacgc tttgagggat tgcctcgtat caaatgcagg
    11641 cgtggcgggg ttttcggggg tcctgacata atcgatttta cctatcaatc gcttgatcgc
    11701 attcccaaaa aaaagttttc gaacctgcgg cgatgcgagg aagggttggc gggcggtccc
    11761 ggacagccag ggctgcaggg atttgacccg cccctcccta caagtgagaa taattatcac
    11821 ctgattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg
    11881 ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg
    11941 ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg
    12001 gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg
    12061 tgctgcaccc agccttcacc gcgatgctta tcgcagtaac ccgaactgtc tgtggttgta
    12121 cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac
    12181 cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc
    12241 tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc
    12301 cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg
    12361 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
    12421 tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca
    12481 tcgagtgcat taacatcacg ctgtctctgc tgcatgtcag taatggttgc gttcgccagc
    12541 ttcagttctc ttgcgttttt gtcgcgctgc tctttgtagg taatggcgtt atcacggtaa
    12601 tgattaacag cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg
    12661 gtgactctgc tcattgctgc ccccacaaac agacttcacg ctcaatctca cgacgggtca
    12721 tcagcccttt ccattgctta ccgccagcat atgtccagcg acgtagctgg tcacatgcgc
    12781 ctt
//
LOCUS       DADLZM010000114        12748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLZM010000114 DADLZM010000000
VERSION     DADLZM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.115"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="JNY73_003083"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="JNY73_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY8049008.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="JNY73_003084"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="JNY73_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY8049009.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPSAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="JNY73_003085"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="JNY73_003085"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY8049010.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="JNY73_003086"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="JNY73_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY8049011.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="JNY73_003087"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="JNY73_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY8049012.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="JNY73_003088"
     CDS             4025..4615
                     /locus_tag="JNY73_003088"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY8049013.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="JNY73_003089"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="JNY73_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY8049014.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="JNY73_003090"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="JNY73_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY8049015.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="JNY73_003091"
     CDS             complement(6551..6658)
                     /locus_tag="JNY73_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049016.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="JNY73_003092"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="JNY73_003092"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY8049017.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="JNY73_003093"
     CDS             7648..7875
                     /locus_tag="JNY73_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049018.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="JNY73_003094"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="JNY73_003094"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY8049019.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="JNY73_003095"
     CDS             complement(9447..10703)
                     /locus_tag="JNY73_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY8049020.1"
                     /translation="MGRNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="JNY73_003096"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="JNY73_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY8049021.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="JNY73_003097"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="JNY73_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY8049022.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..12698)
                     /gene="torD"
                     /locus_tag="JNY73_003098"
                     /pseudo
     CDS             complement(12282..12698)
                     /gene="torD"
                     /locus_tag="JNY73_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
BASE COUNT         3049 a         3243 c         3212 g         3244 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tagtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcgacta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcattttg cgcagccgtg gaatacacca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggctt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg tccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgt gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcttg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta atttgattat
     6301 ggtcgcagta accttcgttc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcgggcaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggtgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttattaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc tcggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattacgacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccgtt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcttcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcact
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgta gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgtggcag gtaatgctgc caacttactg
    12721 atttagtgta tgatggtgat tttaaggt
//
LOCUS       DADLZM010000115        12490 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLZM010000115 DADLZM010000000
VERSION     DADLZM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.116"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            34..435
                     /locus_tag="JNY73_003099"
     CDS             34..435
                     /locus_tag="JNY73_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY8049023.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JNY73_003100"
     CDS             447..818
                     /locus_tag="JNY73_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049024.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="JNY73_003101"
     CDS             805..1395
                     /locus_tag="JNY73_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049025.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="JNY73_003102"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="JNY73_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="JNY73_003103"
     CDS             1802..2542
                     /locus_tag="JNY73_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049026.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="JNY73_003104"
     CDS             2601..2987
                     /locus_tag="JNY73_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY8049027.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="JNY73_003105"
     CDS             2996..3325
                     /locus_tag="JNY73_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY8049028.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..5240
                     /locus_tag="JNY73_003106"
                     /pseudo
     CDS             3297..5240
                     /locus_tag="JNY73_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5237..6338
                     /locus_tag="JNY73_003107"
                     /pseudo
     CDS             5237..6338
                     /locus_tag="JNY73_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6338..6667
                     /locus_tag="JNY73_003108"
     CDS             6338..6667
                     /locus_tag="JNY73_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049029.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6667..7365
                     /locus_tag="JNY73_003109"
     CDS             6667..7365
                     /locus_tag="JNY73_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY8049030.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7370..8113
                     /locus_tag="JNY73_003110"
     CDS             7370..8113
                     /locus_tag="JNY73_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049031.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            8050..8651
                     /locus_tag="JNY73_003111"
                     /pseudo
     CDS             8050..8651
                     /locus_tag="JNY73_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            8712..12191
                     /locus_tag="JNY73_003112"
                     /pseudo
     CDS             8712..12191
                     /locus_tag="JNY73_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            12259..>12490
                     /locus_tag="JNY73_003113"
     CDS             12259..>12490
                     /locus_tag="JNY73_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY8049032.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDE"
BASE COUNT         2949 a         2949 c         3977 g         2615 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgctca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggta agcagtgctg
     3961 cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga
     4021 aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt
     4081 ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac
     4141 tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc
     4201 tgggtctggg taaagcctgg catgacggcg gaaggagggc gaggaattta accgtcagct
     4261 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
     4321 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
     4381 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
     4441 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
     4501 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgactg ccactcagct
     4561 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
     4621 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
     4681 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
     4741 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
     4801 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
     4861 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
     4921 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
     4981 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
     5041 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
     5101 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
     5161 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
     5221 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
     5281 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
     5341 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
     5401 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
     5461 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
     5521 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
     5581 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc ggagggctga
     5641 ggaggtgctg aaggggagcc tgaagcgaca actgaatgaa aacaaacgct actggcagga
     5701 actggaagtg gcgcagggcg actggaaaaa cggtgccatg cgggcgtttc agaattttac
     5761 ggcggatgcg gataatgcgg cgggaacggc agaacagatg tttacagtgg cattcagcag
     5821 tgccggtaat gcactggcga cattctgtac caccggtaag ctgaatttta aatccttcac
     5881 ctcttccctg ttgtcagata tggccagaat tatggcacag atggccatga tgcaggcggt
     5941 aaagggcgtc ggttctttat tcggcttcac gactaatgct gatggcggtg tttaccagtc
     6001 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
     6061 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
     6121 tgctgatggt aagctggggg gttgtggcgg atactggtgg ttcaggcatg gtgatgtttg
     6181 ccccgcagta caacattgag atcaataacg acggcacgaa cgggcagata ggtccggctg
     6241 ccctgaaggt ggtttatgac ctcgggaaaa aagcggccgc ggactttatg caacagcagg
     6301 cccgtgatgg tggccggtta agtggagcat accggtaatg gagacgtttc actggaaagt
     6361 gcgcccggat atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc tgggcgatgg
     6421 ttatgaacag cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca gcgtgacgat
     6481 acgtgttcgt aaaggtgaac acccatcttt aaaagccttt ctggaacggc acggtggcgt
     6541 ccgcgcattt cagtggacgc caccttatga ctggaaactg atcagggagg tttgtcgtaa
     6601 atggtcggca agcgtggggg cgttatgggt gactgtaacg gccgattttg aacaggtggt
     6661 gaactgatgc aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc ggagcagtca
     6721 gcccgggtgg agctctggga aatcgatctg acagaggtcg gtggtgagcg ttattttttc
     6781 tgtaatgagc agaacgaaaa aggtgagccg gtcacctggc aggggcggca gtatcaggca
     6841 taccccattc agggggcggg gtttgaactg aacggcaggg gctgtgctgc ccgtccgaca
     6901 ctgacggtct ctaacctgca cggcatggtc accgggatgg cggaagatct gcagagtctg
     6961 gtcggcggaa cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc
     7021 gtcaacggaa acagcgacgc cgatccggaa caggaggtga tcagccgctg gcgcatcgag
     7081 cagtgcagcg aactgagcgc ggtcagtgcc tcttttgtgc tggccacacc aacggagacg
     7141 gatggcgcgg ttttcccggg gcgtatcatg ctggcgaata cctgtatgtg gacttaccgt
     7201 tctgatgagt gtggttacac gggcagggca gtggctgacg agttcgacaa accaacgacg
     7261 gatatccgga aggacaaatg cagcaagtgt atgcgcgggt gtgagttgcg caacaatacc
     7321 ggtaatttcg gcggtttcct ttccatcaat aaactttctc agtaaatcca tgacacagac
     7381 agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gtggattcgt
     7441 ggtgagaacg ccggagggag acaggtatct tcccagcgag aatatctccg gtgagccgga
     7501 ggaacggttc cggatggctc cggaggactg gctgcgggca caaatgcagg gtgagattgt
     7561 ggcactggtc cacagtcatc ccggtggtct gccctggctg agtgaggctg accggcggct
     7621 gcaggtgcag agtgatttgc cgtggtggct ggtctgccgg ggggcgattc acaagttccg
     7681 ctgtgtgccg catctcaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct
     7741 gttccgggat gcttatcatc tggcggggat tgagatgccg gattttcacc gcggggatga
     7801 ctggtggcgt cacggtcaga atctctatct ggataatctg gaggccacag ggctgtatca
     7861 ggtgccgttg tcatcagcac aaccgggcga tgtgctgctg tgctgttttg gttcatcggt
     7921 gccgaatcat gccgccattt actgcggcga tggtgagctg ctgcaccata ttcctgaaca
     7981 actgagtaaa cgagagaggt ataccgacaa atggcagcga cgcacacact ccctctggcg
     8041 tcaccgggaa tggcacgcat ctgcctttac ggggatttgc aacgatttgg ccgccgcatc
     8101 gaccttcgtg tgaaaacggg ggctgaagcc atccgcgcac tggccacaca gctcccggcg
     8161 tttcgtcaga aactgagtga cggctggtat caggtgcgca ttgccgggcg tgatgcaggt
     8221 gaaaccgaat tgtctgcccg tcttaatgag ccgctggcaa atggtgcagt gatccacata
     8281 gtaccgcgtc tggtgggagc taaaagtggc ggtgtgtttc aggcggtgct gggggcagct
     8341 gttatggcgg ttgctatatg gatgccgggg taggaattat ggcgagtaat ctgctgtttt
     8401 ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg aaagccagaa
     8461 ctccccgtac acagacaacg gataacggca aacagaacac ctgtttctcc tcactggata
     8521 acatggttgc ccagggcaat gttctgcctg ttctgtacgg tgaaatgcgc gtggggtcgc
     8581 gggtggtatc tcaggagatc agcacggcag acgaagggga tggtggtcag gttgtggtga
     8641 ttggtcgctg atgcaaaatg tttcatgtga aaccgcctgc gggcggtttt gtcgtttatg
     8701 gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa gcgaaggaca
     8761 acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg ccggttgacg
     8821 gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg gacagcgagg
     8881 ggaagacaaa cttttccggt gttacggtgg tgttccgcgc cggcgagcag gagcagacac
     8941 cgccggaggg gtttgaatct tccggctcag agactgtgct gggtacggaa gtgaaatacg
     9001 acacgccgat cacccggacc atcacgtcgg caaacattga tcgtctgcgc ctgaccttcg
     9061 gtgtgcaggc actggtggaa accacctcaa agggggaccg gaatccgtcg gaagtccgcc
     9121 tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc accattaagg
     9181 gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg ccgcgcccgt
     9241 ttaatatccg gatgcgtagg atgacaccgg acagcaccac agaccagctg cagaacaaaa
     9301 cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcgc
     9361 tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt cgcaattatc
     9421 atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg cggcaataca
     9481 gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc tggtgtctgt
     9541 gggacatgct cactcatccg cgctaaggca tggggaaacg tcgtggtgcg gcagatgtgg
     9601 acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgccg gacggatttg
     9661 gcggcacgga gccgcgcatc acctgtaacg cttacctgac cacacagcgt aaggcgtggg
     9721 atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc
     9781 tgacgttcgt gcaggaccga ccgtcggata aagtgtggac ctataaccgc agtaatgtgg
     9841 tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaag gaccgccata
     9901 atgtcgttga ggtgaactgg attgatccgg acaacggtca tgagacggcg acagagcttg
     9961 tggaggacac gcaggccatt gtccgttacg gtcgtaacgt cacgaagatg gatgcctttg
    10021 gctgtaccag ccggggacag gcgcaccgcg ccgggctgtg gctgattaaa acggaactgc
    10081 tggaaacgca gaccgtggac ttcagcgtcg gcgcagaagg gcttcgccat gtaccgggtg
    10141 atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt cgcgtactgg
    10201 cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg ccgtcctccg
    10261 gtaccacgct gataagcctg gttgacggaa atggcaatcc ggtcagcgtg gaggttcagt
    10321 ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt gcagaataca
    10381 gcgtgtgggg gctgaagctg ccgacgctgc gccagcgcct gttccgctgc gtgagtatcc
    10441 gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg gaaaaagagg
    10501 ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg aatggtgtca
    10561 cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg gaatatcagg
    10621 tgctggagcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg cttcgcctga
    10681 ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg acggaaacca
    10741 cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg gcggtaaatg
    10801 cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca ccggcagcgc
    10861 ctgtcactat tgaactgata ccggggtatt ttcagataac ggtggtcccg aaactggctg
    10921 tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt gcggatatca
    10981 ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata gccgccagta
    11041 tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac accgttggca
    11101 aatcggcatt tgtggaggcc gtcggtcggg cgagcgatta tgcggaaggt tatctgagtt
    11161 tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg cagattgata
    11221 acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat gtcagcaatg
    11281 aaatcacgca aaccgtcaat aaaacactgg aaaatcagag tgctgcaatc cagcagatac
    11341 agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg aagctgcaac
    11401 agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag aatacgccag
    11461 caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt aatcctgcga
    11521 atggcaacac aaagccgatg tttgttggtc agagcgatca gatattcatg aacgacgtgt
    11581 tcctgaaacg cctgacggct ccgaccatta ccagcggcgg taatcctccg gcattttccc
    11641 tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac gtgaacgcga
    11701 actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg ggaaaattgt
    11761 ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc ccacgggact
    11821 cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat gacgatcagc
    11881 cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa catgagcgga
    11941 agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt
    12001 ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat atgcctgccg
    12061 gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat ggctggtatc
    12121 ccacagcaag tatcagcgat ttgctggttg ttgtgatgaa gaaagccact gcaggcatca
    12181 cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt ttttattgca
    12241 gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc agccatctgt
    12301 ctggctgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct gagcgccggg
    12361 tatcttcagc cccatactga tatgccaggc agcgatgacc tgaagggcat taacgtgaaa
    12421 taccgttatg agtttacgga cacgctgggg ctggtgacgt cattcagcta tgccaacgct
    12481 gaagatgagc
//
LOCUS       DADLZM010000116        12311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLZM010000116 DADLZM010000000
VERSION     DADLZM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.117"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..247
                     /locus_tag="JNY73_003114"
     CDS             <1..247
                     /locus_tag="JNY73_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049033.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNY73_003115"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNY73_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY8049034.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNY73_003116"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNY73_003116"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY8049035.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="JNY73_003117"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="JNY73_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY8049036.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="JNY73_003118"
     CDS             complement(3258..3542)
                     /locus_tag="JNY73_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049037.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNY73_003119"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNY73_003119"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY8049038.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="JNY73_003120"
     CDS             complement(4443..5651)
                     /locus_tag="JNY73_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8049039.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAAAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /locus_tag="JNY73_003121"
     CDS             5735..6271
                     /locus_tag="JNY73_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8049040.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="JNY73_003122"
     CDS             complement(6446..8131)
                     /locus_tag="JNY73_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY8049041.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="JNY73_003123"
     CDS             8401..8778
                     /locus_tag="JNY73_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049042.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNY73_003124"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNY73_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY8049043.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="JNY73_003125"
     CDS             9225..9512
                     /locus_tag="JNY73_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY8049044.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="JNY73_003126"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="JNY73_003126"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY8049045.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="JNY73_003127"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="JNY73_003127"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY8049046.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="JNY73_003128"
     CDS             11269..11745
                     /locus_tag="JNY73_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY8049047.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..>12311
                     /locus_tag="JNY73_003129"
     CDS             12097..>12311
                     /locus_tag="JNY73_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/spermidine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY8049048.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGK"
BASE COUNT         3128 a         3030 c         3111 g         3042 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttacactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
     3721 tgcgacgacc gcgctgccgg catttttcat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcactac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatact
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcggc ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgccctg gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaagc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggtga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg tgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tctgccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggtaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgttacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat t
//
LOCUS       DADLZM010000117        12294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLZM010000117 DADLZM010000000
VERSION     DADLZM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.118"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..1231)
                     /locus_tag="JNY73_003130"
                     /pseudo
     CDS             complement(1..1231)
                     /locus_tag="JNY73_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1430..1514)
                     /locus_tag="JNY73_003131"
     tRNA            complement(1430..1514)
                     /locus_tag="JNY73_003131"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Leu,seq:caa)
     gene            1710..2729
                     /gene="ahr"
                     /locus_tag="JNY73_003132"
     CDS             1710..2729
                     /gene="ahr"
                     /locus_tag="JNY73_003132"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY8049049.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2858..4372
                     /locus_tag="JNY73_003133"
     CDS             2858..4372
                     /locus_tag="JNY73_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY8049050.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGEKKISQT"
     gene            complement(4480..5562)
                     /gene="lptG"
                     /locus_tag="JNY73_003134"
     CDS             complement(4480..5562)
                     /gene="lptG"
                     /locus_tag="JNY73_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY8049051.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKDDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5562..6662)
                     /gene="lptF"
                     /locus_tag="JNY73_003135"
     CDS             complement(5562..6662)
                     /gene="lptF"
                     /locus_tag="JNY73_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY8049052.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6929..8440
                     /gene="pepA"
                     /locus_tag="JNY73_003136"
     CDS             6929..8440
                     /gene="pepA"
                     /locus_tag="JNY73_003136"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY8049053.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8574..9017
                     /gene="holC"
                     /locus_tag="JNY73_003137"
     CDS             8574..9017
                     /gene="holC"
                     /locus_tag="JNY73_003137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY8049054.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9017..11872
                     /gene="valS"
                     /locus_tag="JNY73_003138"
     CDS             9017..11872
                     /gene="valS"
                     /locus_tag="JNY73_003138"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY8049055.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11926..>12294)
                     /locus_tag="JNY73_003139"
     CDS             complement(11926..>12294)
                     /locus_tag="JNY73_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY8049056.1"
                     /translation="RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRS
                     TLTYHGMLYRMCALVVISEITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQ
                     PDKGFLASISRGVMPSLPFL"
BASE COUNT         3030 a         3218 c         3305 g         2741 t
ORIGIN      
        1 tgaggtgagc atatcacctc tgttcaggtg gccaaattca gtgtgccact tcaaacatca
       61 acctacgctc cccaagatgt tccgccttat ggatgtaagc tgctcgcaca gagctacgct
      121 cctggtgact catctgccgc tctaccgcat ccctcgacca taatcccgac tcaatcaacg
      181 aactacaagc catcgtcctg aagccatgcc cacaaacctc gaccttcgtg tcatacccca
      241 ttacgcgcaa agccttgttc accgtattct cactcatcgg cttacgtgga tcgtgatcgc
      301 ccacaaaaat cagctttcga tttccgctca tgcttttgat cttttccaaa atacttaggg
      361 cctgccgcga caaaggtaca aggtgtggtg tccgcatctt cgagccacgc tgagaatgct
      421 tgactccttc cagttgctca cgctcacctg ggattgtcca catagccgtt tcaaaatcta
      481 cttctgacca gcgggcaaaa cgcagctcgc tcgaacggat gaaaaccaat aaagtgagtt
      541 caaccgccag tcgagttaac ggtctgccag aatagtgatc tatgcgatga agtaattcag
      601 gtatacggtt cagttcaaga gctgcacggt gctgtctttt cgccgtagca accgcacctg
      661 caatctcttg cgcggggttg tagtcgatta agccgctctg aacggcaaag cgcataatcg
      721 cggtagtacg ctgctgtaaa cgggcggcaa cttcgagccg cccggatgac tcgactgctt
      781 taatgggtac aagaagatcc cgcgtcttca gttccgcaat gttccgctta ccgatagcag
      841 taaagagatt atcctccaga cttttcaaaa cacgagcgct atgcgatgcc gaccacttct
      901 gattgctggc gtgccagtct ctggcgacta cttcaaacgt tatcgcctct tgttcctgtt
      961 ctaccttaac agctttcttg ttttcactgg gatcgacacc attagctaaa agcttacgag
     1021 cctcatcccg gcgtgccctg gcatccgcca acgacacttc ggggtatttc cccaatgcaa
     1081 gcatcttctc tttgccgcca aagcgataac gtagccgcca gtatttggag ccgttagggt
     1141 gaaccagcaa aaccatgcct tcaccgtcgg tcagcttata ggcttttgct tcaggcttag
     1201 ccgaacgaac cttcacatca ctcagagcca tgatgagtat cctttcaagg gttctgtgtg
     1261 ggtacaaaca ttatcgaacc gggaaatacc cgcagttgta cccgcatcag taagttgatg
     1321 tagattgaat caggttgact taggttgagt gaaaaagcga ggaaagcctt gcggatactg
     1381 gattttaggc acaaaaaaag acgtccgttg acgtctattg atgttccgat ggtgccgaag
     1441 gccggactcg aaccggcacg tatttctacg gttgattttg aatcaactgc gtctaccgat
     1501 ttcgccactt cggcactgaa gaggtatgcg gaaaacgttg tggattatac ctgtcgcacg
     1561 ccaccatgca agcgccagct tgcacccacc tcgctaagtg ctgaaatttt cagcattaca
     1621 ctcagcgata tgtaaccttt gtcacactcc aggccccccg ccctgccatg ctctacactt
     1681 cccaaacaac accagagaag gacccaaaaa tgtcgatgat aaaaagctat gccgcaaaag
     1741 aagcgggcgg cgaactggaa gtttatgagt acgatcccgg tgagctgaag ccacaagatg
     1801 ttgaagtgca ggtggattac tgcgggattt gtcattccga tctgtcgatg atcgataacg
     1861 aatggggatt ttcacaatat ccgctggttg ccgggcatga ggtgattggg cgcgtggtgg
     1921 cactcgggag cgccgcgcag gataaaggtt tgcaggtcgg tcagcgtgtc gggattggct
     1981 ggacggcgcg tagctgtggt cactgcgacg cctgtattag cggtaatcag atcaactgcg
     2041 agcaaggtgc ggtgccgaca attatgaatc gcggtggctt tgccgagaag ttgcgtgcgg
     2101 actggcaatg ggtgattcca ctgccagaaa atattgatat cgagtccgcc gggccgctgt
     2161 tgtgcggcgg tatcacggtc tttaaaccac tgttgatgca ccatatcact gctaccagcc
     2221 gcgttggggt aattggtatt ggcgggctgg ggcatatcgc tataaaactt ctgcacgcaa
     2281 tgggatgcga ggtgacggcc tttagttcta atccggcgaa agagcaggaa gtactggcga
     2341 tgggtgccga taaagtggtg aatagccgcg atccgcaggc actgaaagcc ctgtcggggc
     2401 agtttgatct cattatcaat actgtgaacg tcagcctcga ctggcagcct tattttgagg
     2461 cgctgaccta tggcggtaat ttccatacgg tcggtgcggt tctcacgccg ctgtctgttc
     2521 cggcctttac gttaattgcg ggcgaccgca gcatctctgg ttctgctacc ggcacgcctt
     2581 atgagctgcg aaagctgatg cgctttgccg cccgcagcaa ggttgcgccg acaaccgaac
     2641 tgttcccgat gtcgaaaatt aacgacgcca ttcagcatgt acgcgatggt aaagcgcgct
     2701 accgagtagt cctgaaagcc gacttctgat ccctttgccg ggtgctgtcg cccggcactt
     2761 aaatctcttt ggaaggcgtc tcgcaatctg ctgaatttag cggtgaagag tgcacagccg
     2821 ttgcctatac tccaggcagg aaactggagg aaatctcatg agcgaacccc tgttaattgc
     2881 ccgcacgccg gacacagaac tgtttttact gccgggaatg gctaaccgtc acgggctgat
     2941 tactggcgca acggggacgg gtaaaaccgt tacgctgcaa aaactggcag agtcattgtc
     3001 ggaaatcggc gtgccggtgt ttatggctga tgtgaaaggc gatctgaccg gtatcgcgca
     3061 ggcaggaacg gcgtcggaaa aactgctcac aaggcttaaa aatatcggcg tcaatgactg
     3121 gcaaccgcat gccaatccgg tggtggtgtg ggatatcttt ggcgagaaag gccacacggt
     3181 gcgggcgacg gtttcggatc tggggccgct gttgctggcg cgactgttga atctcaacga
     3241 tgtgcaatct ggcgtgctga atattatctt ccgcattgcg gacgatcagg gattgttgct
     3301 gctcgacttt aaagatttgc gggcgattac ccagtacatc ggcgataacg ccaaatcctt
     3361 ccagaatcag tacggaaata tcagtagcgc atcggttggt gccatccagc gcggattact
     3421 gtcgctggaa cagcaaggcg cagcacactt ctttggtgag ccgatgctgg atatcaaaga
     3481 ctggatgcgc accgatgcca acggtaaagg cgttatcaat atcctcagcg ccgagaaact
     3541 ttatcagatg ccgaaactgt acgccgccag cctgctgtgg atgctttcag agttgtatga
     3601 acaattgccg gaagcgggcg atctggagaa accaaaactg gtgtttttct tcgacgaggc
     3661 acatctgctg tttaacgatg caccgcaggt actgctggat aagattgagc aggtgataag
     3721 gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt ctgatattcc
     3781 ggataatgtg ctcggccagc tcggtaatcg tgttcagcac gctttgcggg cttttacgcc
     3841 aaaagatcag aaagcagtga agacagcggc gcaaaccatg cgggccaatc cggcatttga
     3901 taccgaaaag gcaatccagg aactggggac cggcgaggcg ttaatctcgt ttctcgatgc
     3961 aaaaggaagt ccttctgtgg tggaacgggc gatggtgatc gcaccttgtt cgcgaatggg
     4021 gccggtgacg gaagatgagc gtaatggcct gattaatcac tccccggtgt atggcaaata
     4081 tgaagatgag gtggaccgcg agtccgccta tgagatgctg caaaaaggct ttcaggccag
     4141 taccgagcag caaaataatc cccccgtgaa agggaaagag gtggcggtgg atgacggtat
     4201 tcttggtgga ttgaaggata ttttgtttgg cactaccgga ccacgcggcg ggaagaaaga
     4261 tggtgtggtg caaacaatgg cgaaaagcgc cgctcgccag gtgacgaatc agattgtacg
     4321 cgggatgttg gggagtttgc tgggggggga gaaaaagata agtcagacgt gatctgtgcc
     4381 cctcacccta accctctccc caaaggggcg aggggaccgt tcgaactcgt atttgacttt
     4441 gtcaacaata ataaagggag ctttcgctcc cttgttagat tacgattttc tcattaacag
     4501 ccacaggctg attaagaaga agctggcact tggcaacagt gcgccgatga tcggcgggat
     4561 gccgtaaacc aacgtcagcg ggccgaagat ctggtccagt acgtagaaga caaaaccgaa
     4621 gctgatacct gtgaccacac gcacgcccat cggtacgcta cgcagtgggc caaagataaa
     4681 cgacagcgcc atcagcatca tcaccgccac ggatagcggc tggaagattt tgctccacat
     4741 gttgagctga taacgtccgg catcctgacc gctcgacttc agatacttca cgtagttgtg
     4801 caaaccactg atggagagtg catccgggtc cagcgccacc acgcccagtt tgtctggcgt
     4861 gaggtcggtt ttccaggtgc cgctcaccgt ctgcgaaccg gtaatctgtt tcggattggt
     4921 cagatcagat tcatcaacct gcgacagacg ccagacttta tgttccgggt caaacttcgc
     4981 ggtagcggca taacgtacgg attgcagacg acgattctcg ttaaaggcat aaatactgat
     5041 gccacctaac acctcgtcat ctttaacccg ctcaatgtag acgaagttgt tgccatcttt
     5101 cgcccataaa ccttgctggg tagagagcaa cgagccgccg tacatcgcct gcgcacggta
     5161 gttacgcgcc atctgttcgc cctgcggcgc gacccattcg ccaatcgcca tcgtcagcaa
     5221 gaccagtgga atggcggttt tcatcaccga cagcgctacc tgcatacggg taaaaccaga
     5281 agcctgcatc accaccagtt cgctgcgctg cgccagcatc ccaagaccaa gcaacgcccc
     5341 aagaagagcc gccatcggga agaagatctg cacatctttc ggcacgctca gcaaggtata
     5401 cattcctgcg cctaacgcgt cgtaactccc ctgcccggct tttttcagct gatcgacaaa
     5461 tttgataatg cccgacagcg acaccagcat gaacagcgtc atcatgatgg tggtgaaaat
     5521 agttttaccg atatagcggt caagtacgcc aaaaggttgc atcacaccgc tcctttacgc
     5581 gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag aacaatcgcc
     5641 aaagccagat aaatcaggtt aacggtccac atccacagtg tcgggtccag cttaccttta
     5701 ccgccgttcg atttcaggga ggtctggatc aggaagaaaa gtagatacag cagcatggct
     5761 ggcagcatcg acagtacgcg tccctgacgc gggttaacca cgctcaacgg tacaaccata
     5821 agtgccatca taaacacggt gaataccaac gtgatacgcc agttcagttc tgcgcgagca
     5881 cgatcggtgt cagtgttcca caatgtgcgc atgtccatct ggtcggtatc gttcgggtcg
     5941 agcgccaccg cctggtgacc aatgatcgcc tgataatcct ggaaatccgt aatgcggaaa
     6001 tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac gacctgggag
     6061 ccgtcgcgca gctgggttaa atgtccggaa tcggccacca ccacagaagg acgtgcatta
     6121 ccttttggtc gaatttgtgc gaggaacaca tctttgaaat cgctgccgtc aacgctttcg
     6181 atgaacagca ccgagctgcc attagtcgct tgctggaatt gcccttgcgc cagcgccgcc
     6241 atgccagggt tcgctttcgc ttctgctaac acttcatcct gatgacgcga tgaccacggt
     6301 cccgcccaca tcacgttaac cgccgcgacg attgccgtga ataccgcaag gatcattgcc
     6361 gctttcacca gaaccgcttt gctcaagccg caggcatgca ttaccgtaat ttcactttcg
     6421 gtatacagtt tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg caggataagc
     6481 tgcgccattt ccggcacgcc caacccgaga agggagagca ccagattcgc cggaatatcg
     6541 ccgtcaaccg ctgcgccgag gatcctcact aacttttgac agaagaagat caaaagcaag
     6601 atgaagagta tcgccagctg gcttttgagc gtctcccgca ccagatatct tatgattatc
     6661 actttaaata cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt tcatggctta
     6721 aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa gataattata ctcaacggat
     6781 tcacctctca gattttgttc tgacgtgcca atgccgtaat aacgttaaga ttaacacgaa
     6841 gtcatcgcaa cagcggacat gagttacgaa agcttgcaat tctatctgta gccaccgccg
     6901 ttgtctttaa gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg gtagcccgga
     6961 gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc tttctccgat
     7021 tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc gccctgctac gccggggcga
     7081 actggaagga aaaccggggc agacattgtt gctgcaccat gttccgaatg tactttccga
     7141 gcgaattctc cttattggtt gcggcaaaga acgtgagctg gatgagcgtc agtacaagca
     7201 ggttattcag aaaaccatta atacgctgaa tgatactggc tcgatggaag cggtctgctt
     7261 tctgactgaa ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc aggctgtcga
     7321 gacagcaaaa gagacgctct acagtttcga tcagttgaaa acgaataaga gcgaaccgcg
     7381 tcgtccgctg cgtaaaatgg tgttcaacgt gccgacccgc cgtgaactga ccagcggtga
     7441 gcgcgcgatc cagcacggtc tggcgattgc cgccgggata aaagcagcga aagatctcgg
     7501 taatatgccg ccgaatatct gtaacgccgc ttatctcgcc tctcaggcgc gccagctggc
     7561 tgacagctac agcaagaatg tcatcacccg cgttatcggc gaacagcaga tgaaagagct
     7621 gggaatgcac tcttatctgg cggtcggtca gggttcacaa aacgaatcgc tgatgtcggt
     7681 gattgagtac aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg tgggtaaagg
     7741 tttaaccttc gactccggcg gtatctccat caagccttca gaaggcatgg atgagatgaa
     7801 gtacgatatg tgcggcgcgg cggcggttta cggcgtgatg cgtatggtcg cggagctgca
     7861 actgccgatt aacgttattg gcgtgctggc aggctgtgaa aacatgcctg gcgggcgtgc
     7921 ctatcgtccg ggcgatgtgt taaccaccat gtccggtcaa accgttgaag tgctgaacac
     7981 cgatgccgaa ggccgcctgg tactgtgcga cgtgttaact tacgttgaac gttttgagcc
     8041 ggaagcagtg attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc tgggtcatca
     8101 tattactggt ctgatggcga accataatcc gctggcccat gaactgattg ccgcgtctga
     8161 acaatccggt gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg aacagctgga
     8221 gtccaatttt gccgatatgg cgaacattgg cggtcgtcct ggtggggcga ttaccgcagg
     8281 ttgcttcctg tcacgcttta cccgtaagta taactgggcg cacctggata ttgcaggaac
     8341 cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgt ccggtagcgt tgctggcaca
     8401 gttcctgtta aaccgcgctg ggtttaacgg cgaagagtaa gtttgcgttt taccctcacc
     8461 ctaaccctct ccctaaggga gagggaaccg atcgagcgca atagaaattc attgctcatc
     8521 tttcagggtg aggaatgcag caatatttaa atccaccaca agaagcccca tatatgaaaa
     8581 acgcaacgtt ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc
     8641 aactggtgtg tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgta ctcatcgcct
     8701 gtgaagatga aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa
     8761 gctttgttcc gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg
     8821 cctggccgca aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct
     8881 ttgcagattt tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc
     8941 tgaaacaact ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata
     9001 cggcaacctg gaaataatgg aaaagacata caacccacaa gatatcgaac agccgcttta
     9061 cgagcactgg gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt
     9121 ctgcatcatg atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca
     9181 gcaaaccatc atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg
     9241 gcaggtcggt actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc
     9301 cgcagaagaa ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg
     9361 ggaatggaaa gcggaatctg gcggcaccat tacccgtcag atgcgccgtc tcggcaactc
     9421 cgtcgactgg gagcgtgaac gcttcaccat ggacgaaggc ctgtccaatg cggtgaaaga
     9481 agttttcgtt cgtctgtata aagaagacct gatttaccgt ggcaagcgcc tggtaaactg
     9541 ggacccgaaa ctgcgtaccg ctatttctga cctggaagtg gaaaaccgcg aatcgaaagg
     9601 ttcgatgtgg cacatccgct atccgctggc tgacggcgcg aaaaccgcag acggtaaaga
     9661 ttatctggtg gttgcgacca ctcgcccgga aaccctgctg ggcgataccg gtgtagctgt
     9721 taacccggaa gatccgcgtt acaaagatct gattggcaaa tatgtcattc tgccgctggt
     9781 taaccgtcgt attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg
     9841 cgtgaagatc accccggcgc acgactttaa cgactatgaa gtcggtaaac gtcacgccct
     9901 gccgatgatc aacatcctga cctttgacgg tgatatccgt gaaagcgccc aggtgttcga
     9961 taccaaaggt aacgaatctg acgtttattc cagcgagatc ccggcagagt tccagaaact
    10021 cgagcgtttt gctgcacgta aagcagtcgt tgctgcggtt gatgcactcg gcctgctgga
    10081 agaaattaaa ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tggttatcga
    10141 accgatgctg accgaccagt ggtacgtgcg tgccgatgta ctggcgaaac cggcagttga
    10201 agcggttgag aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc
    10261 ctggatgcgc gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat
    10321 cccggcatgg tatgacgaag cgggtaatgt ttatgttggc cgcaacgaag aagaagtgcg
    10381 caaggaaaat aacctcggtg ccaacgttgc cctgcgtcag gacgaagacg ttctcgacac
    10441 ctggttctct tctgcactgt ggaccttctc tacccttggc tggccggaaa ataccgacgc
    10501 cctgcgtcag ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg
    10561 gattgcccgc atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca
    10621 ggtgccgttc cacactgttt acatgaccgg tctgattcgt gacgacgaag gtcagaagat
    10681 gtccaaatcc aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc
    10741 agaactgctg gaaaaacgca ccggcaatat gatgcagccg cagctggcgg acaaaatccg
    10801 taagcgcacc gagaaacagt tcccgaacgg cattgagccg cacggcaccg atgccctgcg
    10861 cttcaccctg gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgtct
    10921 ggaaggctac cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa
    10981 cacagaaggt caggattgcg gcttcaacgg cggcgaaatg acgctatcgc tggcggaccg
    11041 ctggattctg gcggagttca accagactat caaagcgtac cgcgaagcgc tggacagctt
    11101 ccgcttcgat atcgccgcag gcattttgta tgagttcacc tggaaccagt tctgcgactg
    11161 gtatctggag ctgaccaagc cggtaatgaa cggtggaaca gaagccgaac tgcgcggtac
    11221 tcgccatacg ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc
    11281 gttcatcacc gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac
    11341 catcatgctg cagccgttcc cgcagtatga tgcatctcag gttgatgaag ccgcactggc
    11401 cgacaccgag tggctgaaac aggcgatcgt tgctgtacgt aacatccgtg cagaaatgaa
    11461 catcgcgccg ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg
    11521 tcgcgtaaat gaaaaccgtg gcttcctgca aacgctggcg cgtctggaaa gcatcaccgt
    11581 gctgcctgcc gatgacaaag gtccggtttc cgttaccaag atcatcgacg gcgcagagct
    11641 gctgatcccg atggctggcc tcatcaacaa agaagatgag ctggcgcgcc tggcgaaaga
    11701 agtggcgaag atcgaaggtg aaatcagccg catcgagaac aagctggcaa atgaaggctt
    11761 tgtcgcccgc gcaccggaag cggttatcgc gaaagagcgt gagaagctgg aaggttatgc
    11821 agaagcgaaa gccaagctga ttgaacagca ggctgttatc gccgcactgt aatcaacaca
    11881 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    11941 agaaggcata actccccgag aaatactggc taagaagcct ttatctggtt gttcttcttt
    12001 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    12061 aatcatccat attttcagca gtgggtaagc cagaccaccc gtaatctcgg atatcaccac
    12121 caacgcacac atgcgataaa gcataccgtg gtacgttaaa gttgagcgaa aacgaatacg
    12181 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    12241 ggtgctaaca gcaatcccaa aataataaat taactgcgcg attatcattt tacg
//
LOCUS       DADLZM010000118        12251 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLZM010000118 DADLZM010000000
VERSION     DADLZM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.119"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..860
                     /gene="potF"
                     /locus_tag="JNY73_003140"
     CDS             <1..860
                     /gene="potF"
                     /locus_tag="JNY73_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY8049057.1"
                     /translation="ASFLERQLTAGVFQPLDKSKLPEWKNLDPELLKLVAKHDPDNKF
                     AMPYMWATTGIGYNVDKVKAVLGENAPVDSWDLILKPENLEKLKSCGVSFLDAPEEVF
                     ATVLNYLGKDPNSTKADDYTGPATDLLLKLRPNIRYFHSSQYINDLANGDICVAIGWA
                     GDVWQASNRVKEAKNGVNVSFSIPKEGAMAFFDVFAMPADAKNKDEAYQFLNYLLRPD
                     VVAHISDHVFYVNANKAATPLVSAEVRENPGIYPPADVRAKLFTLKVQDPKIDRVRTR
                     AWTKVKSGK"
     gene            955..2031
                     /gene="potG"
                     /locus_tag="JNY73_003141"
     CDS             955..2031
                     /gene="potG"
                     /locus_tag="JNY73_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY8049058.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            2098..3051
                     /gene="potH"
                     /locus_tag="JNY73_003142"
     CDS             2098..3051
                     /gene="potH"
                     /locus_tag="JNY73_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY8049059.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            3048..3893
                     /gene="potI"
                     /locus_tag="JNY73_003143"
     CDS             3048..3893
                     /gene="potI"
                     /locus_tag="JNY73_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY8049060.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            3953..4441
                     /locus_tag="JNY73_003144"
     CDS             3953..4441
                     /locus_tag="JNY73_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY8049061.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            4482..5609
                     /gene="rlmC"
                     /locus_tag="JNY73_003145"
     CDS             4482..5609
                     /gene="rlmC"
                     /locus_tag="JNY73_003145"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY8049062.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(5784..6515)
                     /gene="artJ"
                     /locus_tag="JNY73_003146"
     CDS             complement(5784..6515)
                     /gene="artJ"
                     /locus_tag="JNY73_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8049063.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(6807..7475)
                     /gene="artM"
                     /locus_tag="JNY73_003147"
     CDS             complement(6807..7475)
                     /gene="artM"
                     /locus_tag="JNY73_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY8049064.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(7475..8191)
                     /gene="artQ"
                     /locus_tag="JNY73_003148"
     CDS             complement(7475..8191)
                     /gene="artQ"
                     /locus_tag="JNY73_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY8049065.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(8198..8929)
                     /gene="artJ"
                     /locus_tag="JNY73_003149"
     CDS             complement(8198..8929)
                     /gene="artJ"
                     /locus_tag="JNY73_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8049066.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(8947..9675)
                     /gene="artP"
                     /locus_tag="JNY73_003150"
     CDS             complement(8947..9675)
                     /gene="artP"
                     /locus_tag="JNY73_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY8049067.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(9893..10408)
                     /locus_tag="JNY73_003151"
     CDS             complement(9893..10408)
                     /locus_tag="JNY73_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8049068.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            11057..>12251
                     /locus_tag="JNY73_003152"
     CDS             11057..>12251
                     /locus_tag="JNY73_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049069.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVK"
BASE COUNT         3036 a         2893 c         3138 g         3184 t
ORIGIN      
        1 ctgccagctt tcttgaacgc cagttgactg cgggagtttt ccagccgctg gacaaaagca
       61 aattgccgga gtggaagaat ctcgacccgg agctgctgaa gctggtcgcc aaacacgatc
      121 ccgacaataa atttgctatg ccctatatgt gggcgacgac cgggattggc tataacgttg
      181 ataaagttaa agctgtgctg ggcgaaaacg cgcccgtcga tagctgggac ttgatcctca
      241 aacctgaaaa tctggaaaaa ctgaaaagct gcggtgtctc tttcctggat gcgccagaag
      301 aagtttttgc taccgtgttg aattatctcg gcaaagatcc caacagcact aaagcggatg
      361 attacaccgg accggcaacc gatctgctgt taaagctgcg cccgaacatt cgttatttcc
      421 attcatctca atacattaac gacctggcaa acggcgatat ttgcgtcgct atcggctggg
      481 caggtgatgt ctggcaggcg tcaaaccgcg tgaaggaagc gaagaatgga gtgaatgtct
      541 cgttctcgat tccaaaagaa ggggcgatgg cgttctttga tgtattcgcc atgcctgcgg
      601 atgccaaaaa caaagacgaa gcctatcagt tcctgaatta cctgctgcgc ccggatgtag
      661 tagcgcatat ttccgaccat gtgttctatg tcaacgccaa taaagcagcc acgccgctgg
      721 tgagtgcgga agtccgtgag aacccgggta tttatccgcc tgcggatgtt cgtgcgaagc
      781 tgttcactct gaaagtgcag gacccgaaaa tcgaccgtgt gcgcacccgc gcgtggacca
      841 aagtgaagag cggaaaataa tccgcagcct tagatgccgg gcgggcgcac cacacccgcc
      901 ggcaattcgc accatcatgg tgcgcttgca cacattcaat gccggagagc agccgtgaat
      961 gacgctatcc ctcgcccgca ggcgaaaacc cgtaaggtgc tgacgccgct attagaaatc
     1021 cgcaacctca ccaaatccta cgatggtcaa catgcggtgg atgatgtcag cctgaccatc
     1081 tacaaaggtg aaatcttcgc gctgctgggc gcatccggct gtggcaagtc cacgctgctg
     1141 cgtatgctgg caggtttcga acaaccttct gccggacaga taatgcttga tggcgtcgat
     1201 ttgtcacagg ttccgcctta cctgcgcccc atcaatatga tgtttcagtc ttacgcgctg
     1261 tttccgcata tgaccgtgga acagaacatc gcttttggcc tgaaacagga caaactaccg
     1321 aaagcggaaa tttccagtcg ggtcaatgag atgctcggcc tggtgcacat gcaggagttc
     1381 gccaaacgta aaccgcatca gctttctggt ggtcagcgac agcgtgtggc cctggcccga
     1441 agccttgcca agcgcccgaa actattactg ctcgacgagc cgatgggcgc gctggataaa
     1501 aagctgcgtg accggatgca gcttgaagtg gtggatattc tggagcgcgt cggtgtgact
     1561 tgtgtgatgg tcacccacga tcaggaagag gcgatgacca tggcggggcg catcgccatt
     1621 atgaatcgtg ggaaatttgt ccagattggc gaaccggaag agatctacga gcatccgact
     1681 acccgctata gcgccgaatt tattggctcg gtaaacgtct ttgaaggcgt actcaaagag
     1741 cgtcaggaag atggcctggt gcttgattcg ccggggctgg tgcatccact gaaagtcgac
     1801 gcggatgcct cggtggtcga taacgtgccg gtacatgtgg cgctgcgccc ggaaaaaatc
     1861 atgctttgcg aagagccgcc cgccaatggt tgtaacttcg cggtggggga ggtgatacac
     1921 attgcctatc tcggcgatct ttcggtgtat cacgttcgtc tgaaaagtgg gcagatgatt
     1981 agcgcccagc tacaaaacgc ccatcgtcat cgtaaagggt taccgaccta gggcgacgaa
     2041 gtgcgtttgt gctgggaagt ggacagcagt gtggtgctga cggtttaagg agcaaagatg
     2101 aatacacttg aacctgctgc ccagtcgaaa ccgccgggcg gatttaagct gtggttgtcg
     2161 cagctgcaaa tgaagcatgg gcgcaaactg gtcattgcgt tgccatatat ctggttgatc
     2221 ttgctgtttc tgctgccatt tctgattgtc tttaaaataa gcctggcgga gatggcgcgc
     2281 gctattccac cttataccga actgatggag tgggctgacg ggcaactgtc catcactctt
     2341 aatctcggta attttctgca actgaccgac gatccgctct atttcgatgc ttatctccag
     2401 tcgctacagg tggctgcgat ttcgacattt tgctgtttac tgatcggcta tccgctggcg
     2461 tgggcggtgg cgcacagtaa gccttcgacc cgtaatattt tattactact ggtgatcctg
     2521 ccgtcgtgga cctcgtttct gatccgcgtt tatgcctgga tgggaatatt aaaaaacaac
     2581 ggtgtgctga ataattttct gctgtggctg ggggttatcg atcaaccgct gaccattctt
     2641 cataccaatc tggcagttta tattggcatt gtttacgcct acgtgccgtt tatggtgctg
     2701 ccgatttata ccgcgttgat tcgtattgat tattcgctgg tggaagcagc gctggatctc
     2761 ggtgcacgac cgctgaaaac gttctttact gtgatcgtgc cgctgactaa aggtgggatt
     2821 attgccggat cgatgctggt gtttatcccg gctgtgggcg agtttgtgat cccggaactg
     2881 ctcggtggcc cagacagcat catgatcggg cgcgtgctat ggcaagagtt tttcaataac
     2941 cgcgactggc cggtggcctc ggcggtagcg atcatcatgt tgctgctgct aattgtgccg
     3001 ataatgtggt tccacaaaca ccagcaaaaa agcgtgggag aacacggatg aataatttac
     3061 cggtagttcg ttcgccctgg cggattgtga ttttgctgct gggctttacc tttctctacg
     3121 cgccaatgct gatgctggtt atctattcgt ttaacagctc gaagctggtg acggtgtggg
     3181 ccggctggtc aacgcgctgg tatggtgagt tattgcgcga tgacgcgatg atgagtgcgg
     3241 ttggttcaag cctgacaatt gctgcctgtg cggcaacggc ggcggcgatc ctcgggacta
     3301 ttgcggcggt ggtgctggtg cgctttggca ggtttcgcgg atcaaatggc tttgccttta
     3361 tgatcaccgc gccgctggta atgccagatg tcatcacggg cttgtcgctg ttgttattat
     3421 tcgtcgcgct tgctcatgcc attggctggc ctgcggaccg cggtatgctt accatctggc
     3481 tggcgcatgt cactttctgt acggcttatg tggcggtcgt tatttcgtcg cgtctgcggg
     3541 aactggatcg ctcgatagaa gaagccgcga tggatctcgg tgcgacgccg ctgaaagtgt
     3601 ttttcgtcat tacgctaccg atgatcatgc ccgcgatcat ttctggctgg ttactggctt
     3661 ttactttgtc gcttgatgat ctggtgatcg ccagctttgt ttccgggccg ggagccacca
     3721 cgttaccgat gctggtcttt tccagcgtgc ggatgggggt gaatccggaa atcaacgccc
     3781 tggcaacatt gatcctcggc gcggtcggaa ttgtcggatt tatcgcctgg tatctgatgg
     3841 cgcgcgcaga aaaacagcgg atacgcgaca tccaacgtgc aagacgtggc tgaagacact
     3901 aaaatttgcc aacctggcta cataatgccg cgcatgtcgc ggcattgttt tcatggaaga
     3961 cgaaacgttg ggatttttta agaaaacatc ttcatctcat gctcgcctga atgtgcctgc
     4021 gctggtgcag gtggcggcgc tcgccattat tatgatccgt ggcctcgacg tgctgatgat
     4081 tttcaatacg ctgggcgtgc gcggtattgg cgagttcatt catcgcagcg tacaaacctg
     4141 gagtttaacg ctggtctttt taagcagtct ggtgctggtt ttcattgaga tctggtgtgc
     4201 gttttcactg gtgaaagggc gtcgctgggc gcgctggcta tatctgctga cacaaatcac
     4261 cgccgcaagt tacttgtggg cggcttcgct ggggtacggt tatccggagc tgttcagcat
     4321 tcccggtgaa tcaaaacgtg aaatcttcca tagcctgatg ctgcagaagc tgccggatat
     4381 gctcatcctg atgctgctgt tcgttccctc gaccagtcgg cggttcttcc agttgcaata
     4441 atgtgtataa tcgtcgcccc tgatgatgtg aaggtcaatg tatgcagtgc gcactttacg
     4501 acgcgggtcg ctgtcgttcc tgtcagtgga taacgcagcc gattccagag caactctccg
     4561 ctaaaaccgc cgatcttaaa aatctgctcg ccgattttcc ggttgaggaa tggtgcgcgc
     4621 cggtgtcagg cccggaacaa gggtttcgta ataaagccaa aatggtggtg agtggtagcg
     4681 ttgaaaaaac actgctcggt atgctgcatc gcgatggcac gccggaagac ctttgtgact
     4741 gcccgcttta tccagcctca tttgcgcccg tttttgcggc gctaaaaccg tttatcgccc
     4801 gagcggggtt aacgccctac aacgtggcgc gtaagcgtgg cgaactgaaa tacattctgc
     4861 tgactgaaag ccagagtgat ggcggcatga tgctgcgctt tgtactgcgt tctgatacca
     4921 agctggcgca actgcgtaag gcgctgccgt ggttacacga acaactaccg cagctgaaag
     4981 ttattaccgt caatattcag ccggtacata tggcgattat ggaaggggag acggagatct
     5041 acctgaccga acaacaggca ctggcggagc gttttaatga cgtaccgctg tggatccgtc
     5101 cgcaaagttt cttccagact aatccggcgg tcgccagcca gttgtacgcc accgcgcgcg
     5161 actgggtacg acagctgccg gttaaacata tgtgggatct gttctgcggt gtggggggct
     5221 ttggtttaca ctgcgcgacg cctgacatgc agttaaccgg gatcgaaatt gcgccagagg
     5281 ccattgcctg tgcaaagcag tcagccgctg aactgggctt aactcgttta caatttcagg
     5341 cgctggactc cactcagttt gccactgctc agggggaggt gccggagctg gtgctggtta
     5401 acccgccgcg ccgcggcatt ggtaaaccgc tgtgtgatta tctttcaacg atggcaccgc
     5461 gttttattat ctactccagc tgtaacgccc aaaccatggc gaaagatatc cgcgaactgc
     5521 caggttaccg catcgaacgg gtacagcttt ttgatatgtt cccgcacacc gcgcactatg
     5581 aagtgttgac gctgctggtg aagcaataaa aaagccacag gtgcggcttc agattgctga
     5641 tcaggtgcgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatca
     5701 tgctaaatca atacattgca gagttgatgt gggccggata agcgcagcgc atcaggcaat
     5761 cttacttttg tcatcagtct caattactgc ggaaaccact ggtcattgat tttctgatat
     5821 gtaccgtcag ctttaattgc tgccagcgcg ttattcagtt tttccagcag agctttgtta
     5881 tccggacgta cagcgatacc caggccggtg ccgaagtatt gcggatcggt cactttttca
     5941 gtcgcaacgc ccagttgcgg attggttttc agccattcgt ttaccaccgc ggtgtcacca
     6001 aataccccat caatacgacc atttttcaga tcgataaagg cattctgata actgtcatag
     6061 gagacagttt tcacttccgg gtgctgatcc tgaatatatt tctggtgcgt cgtaccgttt
     6121 tccatcccaa tgcgtttgcc tttcaggtcg gcaaacgttt tgtaggtatc ttttttggca
     6181 atcacgacgg ctgagttttc atagtagggt gtggtaaacg aaacctgctt gctacgctcc
     6241 ggggtgatat ccataccgga gattacggcg tcatattttc tgaatttcag tgacgggatc
     6301 aggctgtcga acgcgtgatt agtaaaagta cattctgcct gcatttgttt gcacaaggct
     6361 tttgccagat cgatatcaaa gccgacaatc tcattattag cacctataga ttcaaagggt
     6421 ggataggtgg ctgaaacgcc aaaattgatt ttctctgcgg cagaagcacc gaaagtaaag
     6481 gaagcaagta aagcggcaag aactaacttt ttcatgatgg aactcccgtc tgtcaatctt
     6541 atgatttttg gccgtgtctg cggcatggga taacaatgcc accaagtgaa tttatatgca
     6601 ataaacatga ttaaacaatt taaatgaaat aaaaaaagac ggataactta gtgagttgtc
     6661 cgtcttcatt ataagaattt atgcactatg taggccggat aaggcgtcca cgccgcatcc
     6721 ggcacaggca ccgtgctgat gtctgatgcg acgctggcgc gtcttatcag acctacaaac
     6781 ccccccggcg aatgtacgca gctacattaa ttccgccgtt cgaatgccag tgctttgcgc
     6841 tcgatcagac gcatcatcag cgtcagcagg ccgttgacga ccaggtaaat aatccctgcc
     6901 gcaccgaaca ccattacatc gtaggtgcgt ccgtacaaca actggctgta tcccatcact
     6961 tccatcagcg taatggtgta agccagagag gtacttttga acaccagcac cacttcgttg
     7021 gaataagaag agagcgagcg tttaaaggca tacggcagca ggatcgccag cgtatctttt
     7081 ttgctcattc ccagggcgct acaggactgc cactgacctt ctgggatcgc acgaattgca
     7141 ccgtaaaaca gctgcgtggt atacgccgca ctgttcagcg ataacgcaat cagcgcacat
     7201 aaccacggtt ctgacaacaa atgccacagt gccggatact cctgcaacgt cggaaactgg
     7261 cccggcccgt aataaatcag gaagatctgc accagcagcg gcgtaccggt aaacagcgtg
     7321 atataacccc gcaccagcca caccagcacc ggcgttttca gcgtcaggat gatggtaaaa
     7381 atcaatgcca gaatcagtgc cacaatcagc gaggcaacgg ttagcgtcag gctggtgtgc
     7441 agccctttca tcagctcggg taaatactca aacattagct gggcctccgc tcaaaacgtg
     7501 tcgcgcgcag gtcaatgcgt ttgagaatgt actgactgag cagggtgatc accaggtaaa
     7561 tcgccgccgc cacaatgtac caggtaaatg gttcctgggt acgagtagca atgcttttgg
     7621 tttgtaacat taaatcattc acactaatca aactgaccag cgcggtatct ttcagcagca
     7681 ccagccactg gttaccgagg ccaggcagcg catgacgcca catctgcggc atcaccagac
     7741 ggaaaaagat agccgatttc gacagcccca gcgcctgacc agattcccac tggcccaccg
     7801 gtacagcttt caacgcgccg cgcagcgttt gcgaggcata ggcggcatac agcagtgaca
     7861 gagcgatgac accacaaagg aacggactca cgtcgaagtt ctcaatgtcc atctgcaccg
     7921 ggatctgcac gaacccaaga ttgatagtga agccatccga aagcgtcagc agtaactgcg
     7981 aggagccaaa atagataaac agcactacca gaatttctgg caggccacgc agaatggtta
     8041 ccagcgctga acctgcccac gcgacaggac gccatttcgc tgactcccat accgcaaaga
     8101 acatcgccag cgccagcccg acaatcaatg cacaaacggc aaggccgacg gtcatcccgg
     8161 cggcgcttgc taaaggaaaa aattcattca tcaggaatta cttctggaac catttgttgt
     8221 agatggtttc gtaagtgcca tctttcttca ctttttccag cgcagtgttg agtttctgct
     8281 gcagttcagt gttgccctga cgtaccgcga tgccgaggcc agtaccgaaa taatctttat
     8341 cggtcacttt atcgcccact gccgccagtt ttgggttatc tttcagccac tcagtgacca
     8401 ctgcggtgtc accaaatacg ctgtcgatac gcccgttttg cagatccagt tttgcgttct
     8461 ggtagctgtc atacggaacg gtggtgattt ccgggtgctt atccataatg aacttctggt
     8521 gtgtcgtccc gttctgaacg ccgacttttt tgcctttcag ctgatcaaca ctggtgtatt
     8581 tgccttgctg acccacaaac agggcagagt tgtcatagta cggggtagta aacagtacct
     8641 gcttttcacg ctccggagtg atatccatgc ccgccatcac tgcttctacg cgacggaatt
     8701 tcaggcttgg gatcaggctg tcaaacgcct ggttagagaa agtacaggtt gcatcaatct
     8761 ctttacacag tgcctgtgcc aggtcgacgt caaaaccaac gatctggttg tttgcatcaa
     8821 tcgattcaaa cggaggatag gaggcttcgg tagcaaaacg aatggtttcg gcagctgtgg
     8881 cggaaagact aaaacctgca attaacgcgg caatcagaac ttttttcatt gttgttatcc
     8941 cgaatcttag tgagagagat agtttttaaa tgcttcggtt tgcggctcgg taaagcagct
     9001 cgcgtcgcct tgttctacga tatgaccatt ttccatatac accactcggc tggcggtttt
     9061 acgcgccact tcaacttcgt gggtgacgat cacctgggta atattcgttt ctgccagctc
     9121 acgaatgatg ctgacgattt gtgccgtaat ttccgggtcc agtgcggcgg tcggttcatc
     9181 gaacagcagt acctgcggtt ccatcatcaa cgcacgggca atagcaacac gctgctgctg
     9241 accaccagaa agatgcagcg ggtaacgatc gctataaggt ttgagacgca gacgttccag
     9301 cagtttttct gcacgggcca gcgcctgatc tttactcaac cccagtacac ggcagggcgc
     9361 ttcaatcagg ttttgctgca cggtcagatg cggccacagg ttgtattgct gaaacaccat
     9421 gccaacgtta cgacgcaaat cgcgaatcgc tttgtcagag ggtgttttgg tgaaatcgaa
     9481 atggttgcct gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat tgagtacacg
     9541 cagcagcgag cttttacccg cgccgctggg gccaagtaac accagcgttt cgccctgtgg
     9601 gcaatccagc gtgatatcga acagcgcctg atgcgcgccg tagaagcaat taatgccgtt
     9661 taattgaata ctcattgaca ctcgtatact ggcagtctga tagctattga ggtcgaagat
     9721 agtacctttg acagaataat tatgcaatat ttatgcttta aaagttaaaa gcaaagcgca
     9781 ttattcaata aacatagcac aaaataacgg gggcggtggt cggtgagcat aaatgtcggc
     9841 attcctcacg aaatgccgga taatttacgg ggtttattgg ttgatcaagg cgttagcgat
     9901 tctcgatgga ctgacggagc gtacccgccg tggcatgaac gctaccgcct aagtagcgca
     9961 catcgtcgat gacccaacac tggccttcct gaatcattaa cacttcatcc tgccagccct
    10021 ggtcaccctg tttgagatcc acccgcaatg gaatgttacg ggcatcacga ttagggatag
    10081 tcgatgcact ggcaacgtgg gcgctgtctg gcaaggtggt tcgactggag aacggatcgc
    10141 tgcttaacaa ttcacgatga ctattatcgc ggctggcatc gctaagttgg gttgccagtt
    10201 tgtcactcaa ataggggcgc agggcggtaa tgtcattgct gcgatgttga atgcggtagt
    10261 cgtagaattg ttgagccaca ctgtcaggcc ctccctggac acaggaaccg gtgcgggggc
    10321 cgttatcttt ataagctggc gtgacggtgg tacaggcact cagcagcagt gcacatggaa
    10381 taagtagaga tagtgaacgg tagcgcataa tcatttcctt attagcttgc tgtctgcgtc
    10441 acactacgac caaacgctat cagattattt aatacattgt ttttaataca attctgaaac
    10501 aatctgaaca cctgattggg tttgagtcca ttgctggcta cccactcaag gttgctcgta
    10561 gctgacttac ttaaattacc caaacaatag acagtgtaaa ccatttgtga ggttgagcgt
    10621 gttatatggc tgaaatgatc atttaaggta aatatgatag ttctgaaatg gtggtataaa
    10681 tcctcctgta gaggttaaga gcatctgcaa gaagccagtt tcacatttat aacgcgtgat
    10741 aaatgaatgc ggttattgtc taatcattac aaaatttttc acatgcgata agagatcctg
    10801 cgattaagat gattgccaaa attacgttgt atttttgaaa atgagcgaaa tttgatagtc
    10861 ccagctttga ctctcgataa tattatttat gctacaattg tgggtaaaga atttaagcta
    10921 atgaatttga gtgaagaata aattaaattg cttcagtatt tcgttaatta atatgaatac
    10981 aaaaggcgtt gtgcttttat aattataaag cgcgaggagt tcggtggatt atcgtttttc
    11041 ttggatgatt tttaatatga taaacaatat atctgatcaa gcatcatcat ttccaggcac
    11101 acaactaaat caaagtgata atttcctgga ttcactgagg gaatttttcg cgatattaaa
    11161 ttccagtcgt aagggtgagt tgtctacatg ggataccatc tacttgcatc tgatattagc
    11221 aataaatgct gacagtgatc tcataaaaaa tgatgtgttg ctcgcggaaa atattccatc
    11281 agcgaattac caatttaata cattttttag caatgctttc gagattgatg ttaaaaaata
    11341 tctgggtaaa tcagaagata acgaagttga aataaaagcg ggtaatgaaa gaatctctat
    11401 tggaatacgt aatgtatcga atggaaaact tgaaagacaa cagtttcttt tcccactgga
    11461 ctatgagaat aaattacaag aacaattaga taagtatttt acgattgaat cgcacccatt
    11521 attatacagg tataccattg gtagtaagat tgcgaatgtg atttttgaaa agttatacag
    11581 tcgtattgac tttaacaaag aacaatatat atcctttata aaggacgcat ttattcattt
    11641 ttatgattat agcaggcgat atgctattag tgagaatatc gataaagacg ctgtaacaaa
    11701 caatatcgca ttgatgagca ctttttatga cagtgataat acgtcaggtg aggttttaaa
    11761 taatgatttt acagaagaag agtcatttga aactgcgtta gatgtagagc atgcaatagt
    11821 gcttggattt gctgatgata attttgaaac aaaacctgtt cattatcagg atttattaac
    11881 acgatttagt gcctttcaag ataccgtgtt taatctgttt cctgaaatgc actcttcgca
    11941 ttaccacgat atttgtagcg taagtgtgga tatgacaaag ggaacgcaat gcatgattca
    12001 cttgatggtg aatgaagaag ttttcatgtc tctgcctgtt ccagttgcca cgatggtacg
    12061 tgaagatgca agtaatttag tcaatcttaa aacactactc aatgatggtt gttttattaa
    12121 atatagtcat tttaatgacg tggcattaat aaagcaaaac ataagtaatc tctatttatc
    12181 acacactgtt gtgaatgaaa gcatattgaa aaaatgttgt tttgaaaacg gcagtttagg
    12241 tgatgttaaa a
//
LOCUS       DADLZM010000119        12229 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLZM010000119 DADLZM010000000
VERSION     DADLZM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.120"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..493)
                     /locus_tag="JNY73_003153"
     CDS             complement(<1..493)
                     /locus_tag="JNY73_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049070.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JNY73_003154"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JNY73_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY8049071.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="JNY73_003155"
     CDS             complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="JNY73_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY8049072.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2440..3306
                     /gene="folD"
                     /locus_tag="JNY73_003156"
     CDS             2440..3306
                     /gene="folD"
                     /locus_tag="JNY73_003156"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY8049073.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3308..3520
                     /gene="ybcJ"
                     /locus_tag="JNY73_003157"
     CDS             3308..3520
                     /gene="ybcJ"
                     /locus_tag="JNY73_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY8049074.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3628..4149
                     /locus_tag="JNY73_003158"
     CDS             3628..4149
                     /locus_tag="JNY73_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8049075.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="JNY73_003159"
     CDS             complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="JNY73_003159"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY8049076.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5744..6238
                     /gene="ppiB"
                     /locus_tag="JNY73_003160"
     CDS             5744..6238
                     /gene="ppiB"
                     /locus_tag="JNY73_003160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY8049077.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6241..6963
                     /gene="lpxH"
                     /locus_tag="JNY73_003161"
     CDS             6241..6963
                     /gene="lpxH"
                     /locus_tag="JNY73_003161"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY8049078.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7081..7590
                     /gene="purE"
                     /locus_tag="JNY73_003162"
     CDS             7081..7590
                     /gene="purE"
                     /locus_tag="JNY73_003162"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY8049079.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7587..8654
                     /gene="purK"
                     /locus_tag="JNY73_003163"
     CDS             7587..8654
                     /gene="purK"
                     /locus_tag="JNY73_003163"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY8049080.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8791..9684)
                     /locus_tag="JNY73_003164"
     CDS             complement(8791..9684)
                     /locus_tag="JNY73_003164"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY8049081.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9681..10328)
                     /locus_tag="JNY73_003165"
                     /pseudo
     CDS             complement(9681..10328)
                     /locus_tag="JNY73_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10331..11563
                     /gene="allC"
                     /locus_tag="JNY73_003166"
     CDS             10331..11563
                     /gene="allC"
                     /locus_tag="JNY73_003166"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY8049082.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11574..>12229
                     /gene="allE"
                     /locus_tag="JNY73_003167"
     CDS             11574..>12229
                     /gene="allE"
                     /locus_tag="JNY73_003167"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY8049083.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     "
BASE COUNT         2965 a         3168 c         3120 g         2976 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga taaacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa gattgctttg
     1261 gcttcttaca acacatcatt cccttttcat ttaataaagc ggcatgatac aaagtgaacg
     1321 tggtttcgat ttacattgcc accataccgc ttcagacaac cggtacatta ctttgttata
     1381 agacgtgata tataattcaa aacaacgtgg ttttgattat tcgtatctca taataaaagt
     1441 cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg gtgcctttat aacgtgcaga
     1501 aaaatgaaga acgttggtcc cggggatcaa gttctggttg gttgagaagc tattcccatc
     1561 aggtttgaga attgcggatg tattatcaag tatttcaata ccgacacccg ttgctgttgt
     1621 tgtatttgta ctggatgcaa tggccaataa attatcattg attgcatcag cctgaccaga
     1681 aaatgcaacg gcagcattag cggcaacaac aggatcgcag tcgttcaact gaatggtgaa
     1741 tggaattaat gcagaggtat taccaacagc attgaaaata tcggtacgat attgaccgag
     1801 tgtgacaacc tggtctgccg agtcagtatt cactgaacag gcagcattca ccagttcgcc
     1861 ttcaaagtga attgtaccgc catctacaac ggcagcataa ctacccgtag cggcgaatag
     1921 tgcggcagcc agcgcagacg aaataaatct taatttcata tatattcctt caatctcatt
     1981 tatcgactcc acatccgtat ataaccgatt actttattta agacactgat agtagtaaaa
     2041 ttccttttta tcctctaaga atgtcttaat tgaaaatatg cactctattc taaaaaatag
     2101 agagccccgt tagatgaata cttccgcgca aaatatattc aacacaaata tagacctgaa
     2161 gcggtaaatt accaggctga aaattctttt tatattgtca ggtatttctt aaattatctt
     2221 aatccttaaa caaggaaata aatcagttcc agatttacaa cgccatcatg tacgaaaaat
     2281 gaagctttca atctcagcga cctcagcgac ggtgcgccta accttcgcaa gaggtcgctt
     2341 cacgcgataa atctgaaacg aaacctgaca gcgcgccccg cttctgacaa aatagacgca
     2401 tccccttcga cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac
     2461 ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggctcgtatt
     2521 gcagccggac tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg
     2581 caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct
     2641 tatgacctcc cggaaaccac cagcgaagcg gagctgctgg agcttatcga tgcgctgaat
     2701 gccgacaaca ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac
     2761 gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttacaac
     2821 gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc
     2881 acgctgcttg agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca
     2941 tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc tcgcaggttg caccaccacc
     3001 gtgactcacc gcttcactaa aaatctgcgt catcacgtag aaaatgccga tctgttgatc
     3061 gttgccgttg gcaagccagg ctttattccc ggtgactgga tcaaagaagg cgcaattgtg
     3121 attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa
     3181 gacgcggcta aacgcgcctc atacattacg cctgttcccg gcggcgttgg cccgatgacg
     3241 gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacagggt
     3301 gagtaacatg gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct
     3361 gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca
     3421 ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg ccggtcagac
     3481 agtgagtttt gcaggacaca gcgtacaggt tgttgcctga tccctttccc ggcctgtatc
     3541 aacaggccgg ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag
     3601 catcgacatt ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc
     3661 ctttgcattg gtttagggtt gggttcgaaa gtcatccctc cgcgtctgtt atttgccgga
     3721 atcatcctag cgatgctgcc cgatgccgac gtattgtcgt ttaaatttgg cgttgcttac
     3781 ggcaatgttt ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg
     3841 ctcttatgtg tgctgattgg acgtcgatgg ttcagggctg ggctgattcg ctgctggctg
     3901 tttttaaccg tctcgttgct atcgcacagc ttactggatt cggtaaccac tggcggtaag
     3961 ggcgttggct ggctgtggcc gtggtcagat gaacgctttt tcgctccctg gcaggtgatt
     4021 aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt acgggcatca ggtgattatt
     4081 tccgaattga tgtgggtgtg gttgccgggg atgctactga tgggaatgtt gtggtggcgc
     4141 agacgataac cggatgcgaa aactcgcatc cggcaattgc gcaattactt acgacgccag
     4201 gtggtccctt gcgggccatc ttccagcacg atccccatct cgttaagacg gtcacgtgcc
     4261 gcatctgccg ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct
     4321 tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt
     4381 tcttgctcca gcaggcccaa tacagcggaa agtttacgca ggtgagatgc cattgcattc
     4441 gccgctgcca tatcttctgc tttcagacgg ttcacttcac gcgccatatc aaacagcacg
     4501 gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac
     4561 gcttcgccac cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt gtagaggcgc
     4621 tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt agttcagctg gctgcgatag
     4681 tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt agtatttcag cacatcgcgc
     4741 acggtaaaga agttacccag cgatttggac atcttctcgc ggtcaaccat caccattccg
     4801 gagtgcatcc agtagttcac atactgacca tcatgggcac aggtggactg cgcgatttcg
     4861 ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt ggatatcaaa gtggttaccc
     4921 agctgcttgc agttcatggc cgaacattca atgtgccagc caggacgacc cgcgccccac
     4981 ggagacggcc agctcggttc gccctctttc gacatcttcc acagaacgaa gtccatcggg
     5041 ttgcgtttgt catcaaccac gtcaacgcgc gcgccagcct gcagctggtc gagatcctga
     5101 cgcgacagca cgccataagt tggatcggtc gggacgtcga acatcacgtc gccgttgtcc
     5161 gccacataag cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttctgcgata
     5221 tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg
     5281 tgcatttcgg cgatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg
     5341 cgtttgatga ttttgtcgtc gatatcggta atgttgcgca catacttcag tttatagccg
     5401 aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc gtgaccgata
     5461 tgacagagat cgtaaacggt gattccacac acgtacatgc cgacttcccc ggcgtgaata
     5521 ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc
     5581 gtttagacat gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag
     5641 catacattgc gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc
     5701 cgaactgggt tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac
     5761 caatcacggc gatattgtca tcaaaacttt tgacaataaa gcacctgaaa cagttaaaaa
     5821 cttcctggac tactgccgcg aaggttttta caacaacacc attttccacc gtgttatcaa
     5881 cggctttatg attcagggcg gcggttttga accgggcatg aaacaaaaag ccaccaaaga
     5941 accgatcaaa aacgaagcca acaacggcct gaaaaatacc cgtggtacgc tggcaatggc
     6001 acgtactcag gctccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga
     6061 cttcctgaac ttctctggcg aaagcctgca aggttggggc tactgcgtgt ttgctgaagt
     6121 ggttgacggc atggacgtgg tagacaaaat caaaggtgtt gcaaccggtc gtaacggtat
     6181 gcaccaggac gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc
     6241 gtggcgacac tctttattgc agatcttcat ctctgcgtgg aagaaccggc gatcaccgcc
     6301 ggttttctgc gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc
     6361 gatctgtttg aagcatggat tggcgacgac gatcccaacc cactccatcg ccagatggcg
     6421 gcagcgatca aagcggtgtc cgattccggc gttccctgtt atttcattca tggcaaccgt
     6481 gattttctgc tcggcaaacg ctttgcccgt gaaagcggca tgacattatt gccggaagaa
     6541 aaggtgctcg aactttatgg tcgccgggtg ttgattatgc atggcgacac gctgtgcacc
     6601 gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaatgcta
     6661 ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg cgcgaacagc
     6721 aaagaagcca acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgctgtggtc
     6781 agtgcgatgg aaaaacatca ggtgcaatgg ctaatccacg ggcataccca tcgcccggcg
     6841 gtgcatgaac ttatcgccaa ccagcaacct gcttttcgcg tggtactggg tgcctggcat
     6901 acggaaggtt cgatggtgaa agtcacggcg gatgacgttg agctgattca ttttccgttt
     6961 taaaaaaccc acaactttgc tgatttcaca gccacgcaac cgttttcctt gctctctttc
     7021 cgtgctattc tctgtgccct ctaaagccga gagttgtgca ccacaggagt tttaagacgc
     7081 atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg
     7141 gctaccatgc agttcgccgt cgaaatcttc gaaatcctga atgtcccgca ccacgttgaa
     7201 gtggtttctg ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag cgccgaagag
     7261 aacggttatc aggtgattat tgcgggcgca ggcggcgcag cgcacctgcc aggcatgatt
     7321 gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac agagcgccgc actgagcggt
     7381 gtcgatagcc tctactccat cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg
     7441 attggtaaag ctggcgcggc aaacgcggca ttactggcag cacaaattct cgcgactcac
     7501 gataaagagc tacaccagcg tctgaatggc tggcgcaaag cccagaccga cgaagtgctg
     7561 gaaaacccgg acccgcgagg tgcggcatga aacaggtttg cgtcctcggt aacgggcagt
     7621 taggccgtat gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg ccggtcgggc
     7681 tggacgctga accggcggcg gtgccttttc aacaaagcgt gattaccgct gagatcgaac
     7741 gctggccgga aaccgcatta acccgcgagc tggcgcgtca tccggccttt gtgaaccgcg
     7801 atgtgttccc gattattgct gaccgtctga ctcagaaaca gcttttcgat aagctccacc
     7861 tgccgaccgc gccgtggcag ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc
     7921 gtttaggtga actggcgatt gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat
     7981 ggagtttacg cgccaatgaa accgaacagt taccggcaga gtgttacggt gaatgtattg
     8041 tcgagcaggg cattaacttc tctggtgaag tatcgctggt tggcgcgcgc ggctttgatg
     8101 gcagcaccgt gttttatccg ctgacgcata acctgcatca ggacggtatt ttgcgcacca
     8161 gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca agccgaagag atgctgtcgg
     8221 cgattatgca ggagctgggc tatgtgggcg tgatggcgat ggagtgtttt gtcaccccgc
     8281 aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac tggacacaaa
     8341 acggtgccag catcagccag tttgagctgc atctgcgggc gattaccgat ctgccgttac
     8401 cgcaaccagt agtgaataat ccgtcggtga tgatcaatct gattggtagc gatgtgaatt
     8461 atgactggct gaaattgccg ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg
     8521 ggcgtaaagt ggggcatctg aatttgaccg acagcgacac atcgcgtctg actgcgacgc
     8581 tggaagcctt aatcccgctg ctgccgccag agtatgccag cggcgtgatt tgggcgcaga
     8641 gtaagttcag ttaagcaggt gctcatttga ttgccggatg cggcgtaaac gccttatccg
     8701 gcttaccgct ccggtaccca ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     8761 tggtgcaccg attgccggat gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc
     8821 cgccagcgtc tcttcaattc gcgataacgc cccaatccac gcgggtttac tacggcttct
     8881 gacataacca ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg
     8941 cgctaactca tccggtgtgg catggcgaat ggcacgttgc tgcggcgttc cccagttttc
     9001 atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct gctcggcgag
     9061 caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac
     9121 aggcacaccg ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat
     9181 ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccggc gcaaatattt
     9241 accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg
     9301 ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc
     9361 aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca agctctgcgc
     9421 cagcatatag ccaatcatcc cctggctttc cgccaccagc acatccagcg gatacggttc
     9481 tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc cgttgccgtg
     9541 aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag cactggcgat
     9601 attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc
     9661 aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc aagccagaac
     9721 cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca
     9781 gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga ggcgaaatat
     9841 ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgaagattg
     9901 tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata ataccaggcc
     9961 gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg gcctttaccc
    10021 agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac
    10081 tggcgtaatt cagcgggcag cggatcgctt gccgccatcg ccagtggccc gaaaagtccg
    10141 gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga aggcggcgta
    10201 atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct
    10261 tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc
    10321 gtgcggcgaa attacacatt cccgtcaagc tatagaagaa acgctgccct ggctttcctc
    10381 ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg aatggctgga
    10441 aacccagcag caatttaaaa aaagaatggc agcaagtggg ctggaaacac gtttcgatga
    10501 agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag tggttctgag
    10561 cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat tcggcgcgct
    10621 ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcaccgt tacgtacggt
    10681 cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct tctggggcag
    10741 taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg
    10801 aaatagtttt gccgatgcga tgaaggcttg cggatttact ctgcctaacg ccccactaac
    10861 tccgcgtcag gatataaaag cctttgttga actgcatatt gaacagggct gtgtgctgga
    10921 aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc gctatacggt
    10981 gacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc gtcgtgatac
    11041 ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa agatgggcga
    11101 tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa atgtggtgcc
    11161 gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc tgcgcgattt
    11221 cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca ttggtattga
    11281 tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg tcgccaccct
    11341 gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca
    11401 cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc ccagcatcaa
    11461 cgggatcagc cataacccgg cggaacgcac caatattact gaccttgccg aaggggtcaa
    11521 aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca taaatgggat
    11581 atttaaataa cgtcaccggt taccgcgaag atttactggc taaccgtgcg attgttaaac
    11641 acggtaattt cgcactgtta accccggatg gtctggtaaa aaatattatt ccgggctttg
    11701 aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt gttgattatc
    11761 tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa ggcattgaaa
    11821 cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct
    11881 taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt gttaacgccc
    11941 aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta gaaggccatg
    12001 caccgtggct ggtttctggc aatgccagcg aactggaacg cattcactat gaaggtatgg
    12061 atgatgtgat cctgctggat tttctgccca aagagttagg ttttgatatg aacatgcata
    12121 tcctctcttt tgcaccaggt gccagccacg gttatatcga aacacacgtt caggaacatg
    12181 gtgcctatat tctttccggt cagggggttt ataacctcga caataactg
//
LOCUS       DADLZM010000120        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLZM010000120 DADLZM010000000
VERSION     DADLZM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.121"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            56..1327
                     /locus_tag="JNY73_003168"
                     /pseudo
     CDS             56..1327
                     /locus_tag="JNY73_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1340..2110
                     /locus_tag="JNY73_003169"
     CDS             1340..2110
                     /locus_tag="JNY73_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY8049084.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2163..2558
                     /locus_tag="JNY73_003170"
     CDS             2163..2558
                     /locus_tag="JNY73_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049085.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2599..3342
                     /gene="cmoA"
                     /locus_tag="JNY73_003171"
     CDS             2599..3342
                     /gene="cmoA"
                     /locus_tag="JNY73_003171"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY8049086.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGSAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3339..4310
                     /gene="cmoB"
                     /locus_tag="JNY73_003172"
     CDS             3339..4310
                     /gene="cmoB"
                     /locus_tag="JNY73_003172"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY8049087.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4475..6904)
                     /locus_tag="JNY73_003173"
     CDS             complement(4475..6904)
                     /locus_tag="JNY73_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY8049088.1"
                     /translation="MALTIREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAVMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6929..8029)
                     /locus_tag="JNY73_003174"
     CDS             complement(6929..8029)
                     /locus_tag="JNY73_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY8049089.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8417..9163)
                     /gene="cutC"
                     /locus_tag="JNY73_003175"
     CDS             complement(8417..9163)
                     /gene="cutC"
                     /locus_tag="JNY73_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY8049090.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(9177..9743)
                     /locus_tag="JNY73_003176"
     CDS             complement(9177..9743)
                     /locus_tag="JNY73_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY8049091.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9959..11692
                     /gene="argS"
                     /locus_tag="JNY73_003177"
     CDS             9959..11692
                     /gene="argS"
                     /locus_tag="JNY73_003177"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY8049092.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRSLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11869..>12153
                     /locus_tag="JNY73_003178"
     CDS             11869..>12153
                     /locus_tag="JNY73_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAY8049093.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNNHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         3031 a         3066 c         2938 g         3118 t
ORIGIN      
        1 tttaatatca tgaaatatgt aagctcgtat cgctcttggg cgtctggagg tatcgatgga
       61 taaagtcaaa tatccaacag gcgtcgaaaa ccacggcggc acattacgca tctggtttaa
      121 ttttaaaggt aaacgtgtca gggaaaatct tggtgtccct gacactgcca agaacaggaa
      181 gatcgccggg gaactgcgga catcggtatg ttttgccatc cgcacaggaa gctttgatta
      241 tgctgcacag ttccctgact cccctaacct tcaggctttt ggggtaagta aaaaagaaat
      301 tacggtgaag gaacttgaag aaaagtggct ggatctgaaa cgaatggaaa tctctgcaaa
      361 tgcattcaat cgctatgaat ccgttgcaag aacgatggtt ccgaaaattg gaggcggcag
      421 actggtgtca tcggtaacca aagaggaatt gctgtatatc aggaaagatt tgctgaccgg
      481 gtatcagaat tcaacgaaaa acaaagcagc agcaaaagga cggagcgtcg ttactgtaaa
      541 ttattacatg acgacaatcg ctggaatgtt tcagtttgct gcagatcacg gttacttaga
      601 agcaaatccc ttccagggaa ttaagcctct taaaagagcc agggcagagc cagatccgtt
      661 aactcgtgac gaatttattc gcctgataga tgcttgccga catcagcaga cgaaaaacct
      721 gtggtcattg gctgtgtaca caggaatgcg tcacggtgaa ctggtctccc tggcctggga
      781 agatatcgat atgaaagcag gaactattac tatcaggcgc aattatacga aactcggtga
      841 gttcactcta cctaaaactg aagcaagtac aaacagggtt gtgcatctta tccagcccgc
      901 tatcagtgtc ctgaaaaatc aggctgaaat gacaagactg ggtaagcagc accacatcaa
      961 ggttcaacta cgtgaatatg gacgttcagt gaatcatgaa tgtactttcg tatttaaccc
     1021 ccaggtggtt agaaaaagca aacaggtagg ttttatctac aaggtagatt ccattggcga
     1081 ctcatgggaa acagccatta agcgtgcggg catcaggcac cggaaagcat accagtcacg
     1141 acacacttat gcgtgctggt cattatctgc cggagcaaac ccaagcttca ttgccagcta
     1201 gatgggccat gcaagtgccc agatggtgtt caatgtatac ggagcatgga tgactgacag
     1261 taatgcagaa cagatcgcaa tgctgaatca gaagctggca gattatgtcc catggtcacc
     1321 aaagtgacac aagagactta ttaaaatcag tgggttagcc ttcaataccc gtcatgttaa
     1381 ctgcgtggag ggcaacacca cgctttacgc cctgccgaaa cccgaggttg tcctgcgctg
     1441 gcgtgaacag accacagatg acttccgctt ctgttttaag tttccggcga ccatttcgca
     1501 tcaggcagca ttacgacatt gcgatgattt agtgactgaa tttttgaccc gcatgtcacc
     1561 gttggctccg cgcattgggc aatactggct gcaactgcct gccacattcg gcccacggga
     1621 gctgcctgcg ctttggcatt ttctcgattc tctccctggc gaatttaatt atggtgtgga
     1681 agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta atcgcggttt
     1741 acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg catcacgtcc
     1801 acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg tacatgctgt
     1861 actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga cgcaaaaccg
     1921 ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca ctacgcctta
     1981 tcttttttta catacgccag atattgccca ggccccggaa ctggtacata ccctgtggga
     2041 agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc
     2101 tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta aagggagttt
     2161 gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg
     2221 atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt tttagcgaac
     2281 tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgc
     2341 tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg
     2401 ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg tttcgctggc
     2461 gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt
     2521 ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt
     2581 ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga
     2641 ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt
     2701 tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt
     2761 caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac gctctcggtg
     2821 cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc cccggcgatg
     2881 attgaacgct gccgtcgtca tattaacgcc tataaagccc ctacgccagt agacgtcatt
     2941 gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc
     3001 ctgcaattcc tggaaccttc cgagcgccag gcattactgg ataaaattta tcaaggactg
     3061 aacccgggca gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt
     3121 gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag
     3181 atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt ggaaacccat
     3241 aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca gtgctttaac
     3301 tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt
     3361 attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc gcgcagattg
     3421 ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac acggtggaat
     3481 ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc gccgaaagcg
     3541 aagagccact gagcaccggg caaattaaac gcattgaaac gcttatgcgg aacctgatgc
     3601 catggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg
     3661 actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc accattctgg
     3721 atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg
     3781 cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg cgtaaactac
     3841 tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacagctt ccggcactga
     3901 aagcctttga taccgtcttt tcgatgggcg tgctttacca tcgccgttcg ccgctggagc
     3961 atctctggca gttgaaagat caactggtga atgagggtga actggtgctg gaaacgctgg
     4021 ttattgatgg cgatgagaac acggtgctgg taccaggcga tcgttacgcg caaatgcgta
     4081 atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg
     4141 tagatatccg cattgtggat gtgtgcgtta ccactactga agagcagcga cgcactgaat
     4201 ggatggttac cgaatctctg tccgatttcc tcgacccaca tgatccgagt aagacagttg
     4261 aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa aggtctggta
     4321 ttactgccgg atgcggcgtg aacgccttat ccggcctaca aacccttgct aattcaatct
     4381 attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagtttta cgtttgcata
     4441 acctcagcgc ccgtttccgg gcgctattca cttcttacgc ctgaacagca ggcttatcaa
     4501 atgccgttaa cttcggtgcg ttacccgtat atttttcgat atacaccagc gcagagttac
     4561 cggcacaggc atttgccagc tgcgagctgg ggatatccgc cgttaacacg ttcgcactgc
     4621 cgtttttaca caagccgttt tccagatctg gccatgcacc ttcatgcacg cataccacgc
     4681 cttttttgat cccgtcagtc accaccgcgc ctgtcaaaat ctgaccacgt ttgttccaga
     4741 cacgcaccag atcgccattc gcaataccaa atcgagcagc atcttcggtg tgaatagtga
     4801 tcggttcacg atcggcaacc gcatattttt tacgcagttc cgcatagtta agctgactgt
     4861 gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg gcggtaccct
     4921 tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt
     4981 ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca tttttcaccg
     5041 gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg cggcgcattt
     5101 cgatcagttt attttgctgc cagaaggcat taaacattgg catagtgacg cgttgcgcac
     5161 gggccccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca tctttacctt
     5221 cggtatagat ctcttttccg ccaggtttga gtaattccgc aagatcggca aacacgtcaa
     5281 aatcgttacg cgcttcaaat tgcggagcaa cagcctgctt catcggcaca atatgctggt
     5341 tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg atcggtaata
     5401 cgatatctgc gtgtttggct gctgcggtcc agtagcattc agaaacgacg atcatctccg
     5461 gtttctgcca ggctttgatc agacgattag tatcctggtg atgggtaaag ttaccgccac
     5521 ccgcccacca gatcattttg atattcggat acgtttgttc tttaccgtta tgctgatatt
     5581 tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc gtcattccgc
     5641 catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc ggcagcacgc
     5701 cgccaacacg catcgggttg ccaccattgg agtaatgata agagaaacca aaaccgccgc
     5761 ccggtgtacc aatctgccct aacatcaccg ccagcgtcac cagcatccag tgtttctgtt
     5821 caccatattg ctggcgctga attccccagc ctgccatcag aattgtgcga ttcgccgcca
     5881 tcagttcagc gagtttgact atctgcgctt ccggtacgcc agtaatttcc gccgcccaga
     5941 ccgcgctctt cggcgtatta tcgctcttac ctgtcagata ctcttcaaac tgcggatacc
     6001 cggtagtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg
     6061 caatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag gtggcattat
     6121 cgccaaagaa ttcgatggtt tcggagcgga taggatcaat ggcaatcact ggtttgccag
     6181 attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt
     6241 ttaaggtatt cagcgggttc attccccaca gcaccacaac ctggctgttt tccagaatca
     6301 gcggccagga ggcctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca
     6361 cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc gccaggttca
     6421 tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc cagccgtaag
     6481 atccggcaaa aatggccgat ggtccgttag ctttacgaat acgctcatgt tgctcatgaa
     6541 tcagttttaa tgcctgttcc cagctcacct gtacataggt atcttcgcca cgacctttcg
     6601 ccggttgcag tggattgtcg agatagcttt ttctcaccat cggatgctga atacgcgccg
     6661 tggtgtgtac ctgatccgcc gccgtagact gtaaggagtt cggtatggtt ttcgccagcg
     6721 cgcctgtcga agaaacaatc ttgccgtcct tcacttctac gctcatcgct ccccaacgtc
     6781 ccgcggtgag gattttaccg cccttctctt ctgcccatgc ggggagcggt gctgccgatg
     6841 tcaccaccag cgctccagcg gcaataccgc tgtgtttaat aaattcacgt attgttaatg
     6901 ccataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct ttggcgttgt
     6961 actggaaata ccgcgttaaa atgtccagtt cgttttcgct catgctggtt cgtgccccca
     7021 ttcctttggc aatggacggc cacgcattga cggtgtaatg gtcggcggca atagaggcat
     7081 gacaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc ggtttacggt
     7141 ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat tgattgccgt
     7201 attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct tcacttaatg
     7261 tggctagcgc cagccgttga cctgccgcca ggtagagcgt gttttcactg ccctgcattt
     7321 gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg gtgagatcgg
     7381 taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga taaatatgtg
     7441 cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg ttgtcatcca
     7501 tttttatttc tggcggaaaa tgggcaatgc ctttatgaca atcgatacag gtttcgccgc
     7561 ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca atatccatgg
     7621 catcaaaaga atggcaacta cggcacgttg cagagtcagt tgcttttaat tctttccata
     7681 ctgtttcggc catttcctgg cgatgagttt cgaacttatc gtcactgtct attttgccgc
     7741 taacaaattc atgataaata tctttagatg cttttaattt agcaaataaa taatccatcc
     7801 ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg ttcgaaaagt
     7861 ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg
     7921 ctgtatccga cgttttatgt aaggcttttt gcgccagcaa taacccgccg ccaccaacca
     7981 caaccgctat cagcaaaaac aataacccaa tgcgtttttt ccctcgcata gattcctcta
     8041 aataaattcc agaacaccga aatcattgta taattaaata cacaacgaat taaccgcttg
     8101 cataactcgg cacaacactg cctgaaataa tcgataaaga atatgattta ttacaatgta
     8161 atcattaatt gctaaggaat aacccagttg ctatttagaa tatatattta tcagttttag
     8221 caatttaaat cccataatca atgtgaatat aaaaaatatt tttagcacaa cgctatgcgc
     8281 actgtcaccc gctcaacaga tcacggttat cgttcgtttt ttatactgct cagggataaa
     8341 aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat
     8401 gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa
     8461 ccgccgcccc gtctacgata taacgcgaat actcgtccgc gtgttcatct gatgacatgg
     8521 acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga
     8581 cttccagcac tccggcatcg aggaagtggt gcaggttttc tgcacggact cctgctccag
     8641 ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca
     8701 gtgcgtctga tttttgccct gatgtcagta cccgggcaat gcccaattcc gcaagattat
     8761 tgagtgtata taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg
     8821 gaccggcagc agccattatt ttttccattc gtggcatatc gacattccca tcaacatcga
     8881 gaacgcccgt caccagtcca ggaaaaccta attcgcggac cgtgcgcaca tcctcaagaa
     8941 tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga attatcggat
     9001 gcacagggat cgtcacccgc tggcgcacgg atttcagtcc ccccaacgac ggcgttaagc
     9061 ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc tgcgccgtta
     9121 gtgcacattc catgctgtaa cagcaaattt ccagtaatgc catttttgct ccttaattac
     9181 gctgactgct cgctggcaac gatctcttca atggaccacg gatgaaactt aatagttgtt
     9241 ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct catgctcacc ttttggtgaa
     9301 ctggttttaa ctgagatccc cggtaggctg agtccttcat cagaaagcaa tgccagcgca
     9361 cgggcattca gcgtttctgg atcacccggc agaacgattt caatatgttc ccaaccttcg
     9421 tgtgggtaac gtttttcccc aggccacggt agctccacat tggaaaactg ccagtgcgca
     9481 acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat cattttttct
     9541 gacaaaagct cgccacactg ttcaaaaccg cgacgccagc gctcagcggt gacgttttga
     9601 tggcaacgca aagaaatgtg atcggcagtc agcggagtga tatccaaccc cagacggcgg
     9661 gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg
     9721 tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc gttaatttac
     9781 cctgttgccc tgtgccaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa
     9841 cggcaatacg ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccaccc
     9901 taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag gtattccggt
     9961 gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc
    10021 tgcggattgc gaaccgcaga ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc
    10081 taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca
    10141 ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg
    10201 ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc
    10261 gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc
    10321 gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta ttggtgacgc
    10381 agcagtgcgt agtctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga
    10441 ctggggcact cagttcggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc
    10501 cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga
    10561 cgaagatgaa gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga
    10621 cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccaaat
    10681 cacttacgat cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta
    10741 caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag
    10801 cgaaggggcg accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg
    10861 cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc
    10921 gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg tattacatcg actcccgtca
    10981 gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc
    11041 cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa
    11101 aacccgcgcg ggtggtacag tgaaactggc agacctgctg gatgaagccc tggagcgtgc
    11161 acgtcgtctg gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc
    11221 taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga
    11281 ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca
    11341 gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaagca gaaattaacg aagagcaact
    11401 ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct
    11461 gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg taatgtgtgc
    11521 ttacctgtac gatctggccg gtctgttctc tggcttctac gagcactgcc cgatcctcag
    11581 cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac
    11641 gctgaagctg ggtctggata cgctgggtat tgagactgta gagcgtatgt aatcgatttt
    11701 tcgtgagagt gaagcctgat cagggttagc cgatcaggct tttttattgc catctaaatg
    11761 tattctgaaa acggacatgc cattgttttc tcactgttga gtaagaggcc agaagcgtaa
    11821 tatccaggcc caggaaaacg ataactgttc aatttaagga ataccgaagt gtttaaattt
    11881 cttgtattaa cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagta
    11941 aacaaccatg acacttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact
    12001 gacattaacg tgatcaaatc atgcctgcga caagaataca atctcgtcga tacgcaatta
    12061 aataaagcct atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgcaaaa
    12121 aaacttgata ctgaacaact taacttgctt aaa
//
LOCUS       DADLZM010000121        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLZM010000121 DADLZM010000000
VERSION     DADLZM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.122"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            128..997
                     /locus_tag="JNY73_003179"
     CDS             128..997
                     /locus_tag="JNY73_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049094.1"
                     /translation="MAISIKGVNTGVIRKGNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="JNY73_003180"
     CDS             complement(1200..1553)
                     /locus_tag="JNY73_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY8049095.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNY73_003181"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNY73_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY8049096.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="JNY73_003182"
     CDS             complement(3381..3674)
                     /locus_tag="JNY73_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY8049097.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="JNY73_003183"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="JNY73_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY8049098.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNY73_003184"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNY73_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY8049099.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="JNY73_003185"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="JNY73_003185"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY8049100.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /locus_tag="JNY73_003186"
     CDS             7589..8890
                     /locus_tag="JNY73_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8049101.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="JNY73_003187"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="JNY73_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY8049102.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /locus_tag="JNY73_003188"
     CDS             9320..11017
                     /locus_tag="JNY73_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY8049103.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="JNY73_003189"
     CDS             11054..11629
                     /locus_tag="JNY73_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8049104.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="JNY73_003190"
     tRNA            11736..11811
                     /locus_tag="JNY73_003190"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2924 a         3134 c         2989 g         3106 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agggtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgattg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa caccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgttagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atataatatc
     7561 aacttgttaa agaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aactatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10681 aacgatagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggaccc ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aac
//
LOCUS       DADLZM010000122        11931 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLZM010000122 DADLZM010000000
VERSION     DADLZM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.123"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..2132)
                     /locus_tag="JNY73_003191"
                     /pseudo
     CDS             complement(1..2132)
                     /locus_tag="JNY73_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2276..2899)
                     /locus_tag="JNY73_003192"
     CDS             complement(2276..2899)
                     /locus_tag="JNY73_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY8049105.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="JNY73_003193"
     CDS             complement(2896..4584)
                     /locus_tag="JNY73_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY8049106.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="JNY73_003194"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="JNY73_003194"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY8049107.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="JNY73_003195"
     CDS             7507..8229
                     /locus_tag="JNY73_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY8049108.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="JNY73_003196"
                     /pseudo
     CDS             complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="JNY73_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2766 a         3310 c         2942 g         2913 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg aacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagaccg tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttatcc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc taggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctgg ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaactg
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcctcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctaatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag agacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata ataattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccggcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcacccgcta aacaagccgc
     9061 cgctaagcag caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcacc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga aggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccctcacgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gcgaaacatg gcgttagtga
    11641 cacgtaaatc gacggcacca gtatcattag taccagaaga atagattgcg ccactatagc
    11701 cgcctgcgac gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag
    11761 caaatatggc cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca
    11821 ttccagtttc attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc
    11881 caccgctggc attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DADLZM010000123        11882 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLZM010000123 DADLZM010000000
VERSION     DADLZM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.124"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1370
                     /gene="mxiD"
                     /locus_tag="JNY73_003197"
     CDS             <1..1370
                     /gene="mxiD"
                     /locus_tag="JNY73_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY8049109.1"
                     /translation="SKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGP
                     PALVELVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATV
                     VERLLNNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPE
                     TNSILVKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFG
                     DSFGASFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNR
                     TFYVSLVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNEN
                     TSVLPEVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKT
                     SNISNIVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLV
                     SYLNY"
     gene            1389..2456
                     /gene="mxiC"
                     /locus_tag="JNY73_003198"
     CDS             1389..2456
                     /gene="mxiC"
                     /locus_tag="JNY73_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY8049110.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            2469..4529
                     /gene="mxiA"
                     /locus_tag="JNY73_003199"
                     /gene_synonym="virH"
     CDS             2469..4529
                     /gene="mxiA"
                     /locus_tag="JNY73_003199"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY8049111.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            4542..4943
                     /gene="spaK"
                     /locus_tag="JNY73_003200"
     CDS             4542..4943
                     /gene="spaK"
                     /locus_tag="JNY73_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY8049112.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            4947..6239
                     /locus_tag="JNY73_003201"
     CDS             4947..6239
                     /locus_tag="JNY73_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY8049113.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            6332..6670
                     /gene="spaM"
                     /locus_tag="JNY73_003202"
     CDS             6332..6670
                     /gene="spaM"
                     /locus_tag="JNY73_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY8049114.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            6657..7535
                     /gene="spa32"
                     /locus_tag="JNY73_003203"
     CDS             6657..7535
                     /gene="spa32"
                     /locus_tag="JNY73_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY8049115.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            7529..8410
                     /gene="spaO"
                     /locus_tag="JNY73_003204"
     CDS             7529..8410
                     /gene="spaO"
                     /locus_tag="JNY73_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY8049116.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            8415..9065
                     /gene="spaP"
                     /locus_tag="JNY73_003205"
     CDS             8415..9065
                     /gene="spaP"
                     /locus_tag="JNY73_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY8049117.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            9080..9340
                     /gene="spaQ"
                     /locus_tag="JNY73_003206"
     CDS             9080..9340
                     /gene="spaQ"
                     /locus_tag="JNY73_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY8049118.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            9340..10110
                     /gene="spaR"
                     /locus_tag="JNY73_003207"
     CDS             9340..10110
                     /gene="spaR"
                     /locus_tag="JNY73_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY8049119.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            10117..11145
                     /gene="spaS"
                     /locus_tag="JNY73_003208"
     CDS             10117..11145
                     /gene="spaS"
                     /locus_tag="JNY73_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY8049120.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            11158..11400
                     /locus_tag="JNY73_003209"
     CDS             11158..11400
                     /locus_tag="JNY73_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049121.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         3796 a         1678 c         2322 g         4086 t
ORIGIN      
        1 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
       61 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
      121 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
      181 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
      241 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
      301 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagcccaaa
      361 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
      421 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
      481 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
      541 tcactcagtt agatgtagcc aaacgacata tagaattatc attatggata atcgatattg
      601 ataaatctga attaaataac cttggtgtta attggcaagg aacggcttca tttggtgatt
      661 cgtttggtgc gtcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
      721 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
      781 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
      841 tgtcattagt tggtgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
      901 atgttatacc tcgcttttca tctcggggac aaattgagat gtctttaacg atagaggatg
      961 gaacaggaaa tagtcagtca aattacaatt ataataatga aaatacatct gtccttccag
     1021 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
     1081 taggtggtta tacgcatgaa acgaactcta acgaaatagt atctattcct tttctctcct
     1141 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag taatatcagt aatattgtaa
     1201 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
     1261 ataaaagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
     1321 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
     1381 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
     1441 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
     1501 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
     1561 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
     1621 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaacgttaa
     1681 aagataacct tcctctagat cgggatttta tagacagact aaagagatat tttaaagatc
     1741 caagtgatca agtcttagca ttaagggaac ttttgaatga aaaagatctt actgctgaac
     1801 aagtcgaatt attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
     1861 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
     1921 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
     1981 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
     2041 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
     2101 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gacgtaattt
     2161 ttatgaagaa actggaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
     2221 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
     2281 ctgttataga agtatcacaa gccagtcatg aggattctgt agtgtatcaa acatatctat
     2341 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
     2401 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
     2461 agataaaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
     2521 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
     2581 attttttaat agggcttaat attgtacttg ctattctcgt ttttatggga tcattttata
     2641 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
     2701 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
     2761 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
     2821 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
     2881 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
     2941 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
     3001 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
     3061 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
     3121 aacatggaat gtccctatct ggtgcattat ctacttatac catactaact attggtgacg
     3181 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
     3241 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
     3301 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
     3361 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
     3421 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
     3481 atatcgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
     3541 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataaatgca aatgatatgg
     3601 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
     3661 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
     3721 aggtgcgtgc cgatagtttt aatatatatt ttgacaaagt gtgtattaca gatgaaaatg
     3781 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
     3841 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
     3901 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
     3961 ttggtataca agaaacaaaa aatatgttag atcagtttga aaatcggtat cctgatctat
     4021 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
     4081 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
     4141 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
     4201 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
     4261 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
     4321 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
     4381 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
     4441 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
     4501 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
     4561 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
     4621 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
     4681 ttgtcaatga gcaggttatg ttatgggcta attttgatgc accaagtgat gtaaaacttc
     4741 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
     4801 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
     4861 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
     4921 aaatcttaaa tgaggtctta taatcaatga gctatacaaa attgctcact caattatctt
     4981 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
     5041 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tgttgcaaga gctcaggttg
     5101 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
     5161 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
     5221 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acggataaca
     5281 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
     5341 ttgagaagcc ttttttaaca ggtattaagg ttattgattc tttactcacg tgtggtgaag
     5401 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
     5461 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
     5521 aggttacaga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
     5581 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
     5641 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
     5701 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
     5761 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
     5821 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
     5881 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
     5941 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
     6001 ttacacaggt tgttgatgaa aaacatcgta ttatggccgc tgcatttcgg gagctacttt
     6061 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
     6121 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
     6181 atcgactcgg ttttacttat gagcagacaa tggagcttat tggtgaaaca attagataag
     6241 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
     6301 acactaaaaa aaaaactctt tgtatcgaga tgtggaggca ttagataaaa ggattatata
     6361 cttcctgcaa ctggagaatg acttagaacc tgtaggcgct cagagcgtta gtcagctttt
     6421 caatacaaga cgtaagatcg ctatagtaaa aaagcatatt atccaatatc agtctgaacg
     6481 aattcttcta aagggaagaa ttgaagaaat tcaaaaagat attgatgagg caaatgcttc
     6541 taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa cggattggct tgattaaaag
     6601 aaataacttt gccaaacagt tgatacttga tgagttatca caagaggata tgaagtatgg
     6661 cattagataa tataaaccta aattttagta gtgacaaaca gatagaaaaa tgtgagaaac
     6721 tatcttcaat agataatatt gatagtctcg ttttgaagaa gaagaggaag gttgaaattc
     6781 ctgagtactc tttaattgca tctaactatt ttactattga taagcacttt gaacataagc
     6841 atgataaagg agaaatttat agtggcatta aaaatgcgtt cgaacttaga aacgaacgag
     6901 cgacatattc tgatattcca gaatcaatgg ccattaaaga aaatattttg ataccagatc
     6961 aagatatcaa agcaagggaa aaaataaata tcggcgatat gagggggatc ttttcatata
     7021 ataagagtgg aaatgcagac aagaacttcg aaagaagtca tacttcttct gtaaaccctg
     7081 ataatctgct agaatctgat aatagaaatg gtcaaattgg tttaaaaaat catagcttgt
     7141 ctattgataa gaatattgct gacatcattt ctttactaaa tggaagtgtt gctaaatcat
     7201 ttgagctgcc tgtaatgaat aaaaatactg cagacataac cccatccatg tcattgcaag
     7261 aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa aaatagtgaa atgacttacc
     7321 actttaaaca gtggggggct ggacattctg ttagtatttc agtggagtct ggttcttttg
     7381 ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga cttaattttg aaacaagatg
     7441 ctgagggtaa ttacagattt gatagcagtc aacataataa ggggaataaa aataatagta
     7501 caggatataa tgaacagagt gaagaagaat gctaagaatt aaacattttg acgctaacga
     7561 aaaactacag attttatatg caaagcaact ctgcgagcgt ttttcgattc agacattcaa
     7621 aaataaattt acaggcagtg aaagtttagt cactcttact tccgtgtgtg gggattgggt
     7681 aattcgtatt gatacattat cttttttgaa aaaaaaatac gaggtatttt caggattttc
     7741 tacacaagaa tctttactgc atttatcaaa atgtgtcttt atagagtcgt catctgtatt
     7801 ttcgattcca gaactgtctg ataagattac tttccggatc acgaatgaaa tccagtatgc
     7861 aactactggg agtcatttat gctgtttttc ctcttcttta ggtattattt attttgacaa
     7921 gatgccggta ttacgtaatc aagtttctct tgacttattg catcatcttt tagagttttg
     7981 cttaggttca tctaatgtaa ggctggctac tttaaaaaga attcgcactg gtgatataat
     8041 catagttcag aaactttata atttattatt gtgtaatcaa gttattattg gggattatat
     8101 tgtgaatgat aataatgagg caaaaattaa tctgtcagaa agtaatggtg agtcagaaca
     8161 cacagaagtt tctttggcat tattcaatta tgatgatatc aatgtaaaag tggactttat
     8221 tcttttagaa aaaaatatga caatcaatga actaaaaatg tatgtagaaa acgaattatt
     8281 caagtttccc gatgacatag ttaaacatgt aaatattaaa gtaaatggtt ctttggttgg
     8341 gcatggggaa cttgtttcta ttgaggatgg ttatggtatc gagattagtt cttggatggt
     8401 aaaggagtaa tctcatgctg agtgacatgt ccctcatagc tacactttct ttttttacgc
     8461 tattaccatt tttagttgct gctggtacct gttatataaa gttttctatt gtttttgtaa
     8521 tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa tatgactctc aatggtattg
     8581 cattgattat ggctttgttc gtgatgaaac ccattattga agctggttat gaaaattatc
     8641 taaatggccc tcaaaaattt gatacaatat ctgatattgt ccgtttttct gattctggct
     8701 tgatggaata taagcaatac ttgaaaaagc atacagatct tgagttagca cgttttttcc
     8761 aaaggtctga agaagaaaat gctgatttaa agagtgcaga gaataatgat tattcattgt
     8821 tttccctctt gcctgcatat gcgctaagtg aaatcaaaga tgcgttcaaa ataggttttt
     8881 atttatattt gccatttgtt gttgttgatc ttgttatttc aagcatttta cttgcacttg
     8941 gtatgatgat gatgagtcca atcacaatat cagtaccaat aaaacttgta ttatttgttg
     9001 cgcttgatgg gtggggtatc ctgtctaagg ccttgattga acaatatatc aatattcctg
     9061 cttagatgat ggaggtaatg tgtctgatat agtttatatg ggtaataagg ctctttattt
     9121 aatccttatc ttttccttat ggccagtagg tatagctacg gttattggat tatctattgg
     9181 tttgttacag acagtgactc aactccaaga gcagacactt ccttttggta taaagcttat
     9241 aggtgtatca atatctttgc tacttctttc tggatggtat ggtgaggttt tattgtcttt
     9301 ttgtcatgaa ataatgtttt taattaagag tggggtttga tggacatttc aagctggttc
     9361 gaaagtattc atgtgttttt aatactcctg aacggcgttt tttttagatt ggctccatta
     9421 tttttctttc ttccattttt aaataacggt ataatttctc catctattag aatacctgtg
     9481 atttttcttg ttgcatcagg attaattact tctggtaagg tagacatagg ttcttctgtt
     9541 tttgaacatg tttatttcct tatgttcaag gaaataattg ttggcctcct tctctctttt
     9601 tgcttgtctc ttcccttttg gatatttcat gctgttggta gcattattga caaccagcgt
     9661 ggggcaacgc ttagtagttc aattgatcct gccaatggtg ttgatacgtc tgagttggca
     9721 aaatttttca atcttttttc tgcagttgta tttctataca gtggtggtat ggtctttatt
     9781 ttagaatcca tacaattgtc ttataatata tgcccgttat tttctcaatg ttctttccgt
     9841 gtctcaaata tcttaacatt tctgacttta ttggcaagtc aggctgttat tttagccagt
     9901 cctgttatga tagtattgtt actatcagaa gtattacttg gtgtattatc gagatttgct
     9961 ccgcagatga atgctttttc cgtatcatta actattaaaa gtttacttgc aatatttatt
    10021 attttcatct gttcttctac tatttacttt tctaaagttc aatttttcct cggtgaacat
    10081 aaatttttca caaatctatt tgttagataa aatattatgg caaataaaac agaaaagccg
    10141 acacctaaaa aactaaagga tgccgcaaaa aaaggacagt catttaaatt taaggattta
    10201 acgactgttg ttattattct ggtagggaca tttactataa tatcattctt ttccttaagt
    10261 gatgtaatgc tcttatacag atatgtaata attaatgact tcgaaattaa tgagggtaaa
    10321 tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact ttttttctgt
    10381 gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc gactaaagct
    10441 atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat atttagtata
    10501 aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc attaacaacc
    10561 tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc tagtgttgat
    10621 ggcttatatc ttatttgggg ggggctgttt aaggatataa tattattttt cttggcattt
    10681 tctatttttg ttattattct tgactttgtg attgagttca ttttatacat gaaagatatg
    10741 atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca ctttgagaca
    10801 aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa atctgatata
    10861 cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat ttattttaat
    10921 ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg tgccttggct
    10981 gtcagaaaat atgcaaatga agttggtata ccgactgtgc gtgatgtgaa attagctaga
    11041 aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt ggatgaagtc
    11101 ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt aaaggagatg
    11161 tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga atttgaaata
    11221 gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc tttccttagc
    11281 aataaggata taataaatgt tttactcaga aagatttcat ctgaatgtga cctggttaga
    11341 aaagacattt atcgttgtgc tctggagttg gtcgttgaaa aaacacctga tgatttataa
    11401 tcattcataa taactctttt gctgatataa atatttttgt tgaagatatt tatgtagtca
    11461 caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgcactaaa taatatatga
    11521 taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat aagagagact
    11581 attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca aagtctgtaa
    11641 atgatcatga gatactaacc gagagtaatc gtttattatc tcattgcatt tcagatagta
    11701 acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt aaaaggagaa
    11761 tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt aaactatatt
    11821 gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt ttttgatatg
    11881 tt
//
LOCUS       DADLZM010000124        11785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLZM010000124 DADLZM010000000
VERSION     DADLZM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.125"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            202..537
                     /locus_tag="JNY73_003210"
     CDS             202..537
                     /locus_tag="JNY73_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY8049122.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="JNY73_003211"
     CDS             630..713
                     /locus_tag="JNY73_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049123.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="JNY73_003212"
     CDS             813..1634
                     /locus_tag="JNY73_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY8049124.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNY73_003213"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNY73_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY8049125.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNY73_003214"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNY73_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY8049126.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="JNY73_003215"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="JNY73_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY8049127.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMEMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNY73_003216"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNY73_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY8049128.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNY73_003217"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNY73_003217"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY8049129.1"
                     /translation="MQIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="JNY73_003218"
     CDS             7279..8223
                     /locus_tag="JNY73_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8049130.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="JNY73_003219"
     CDS             8409..9791
                     /locus_tag="JNY73_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8049131.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="JNY73_003220"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="JNY73_003220"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY8049132.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="JNY73_003221"
     CDS             complement(10547..10882)
                     /locus_tag="JNY73_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY8049133.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKKKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="JNY73_003222"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="JNY73_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY8049134.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2995 a         3010 c         2834 g         2946 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggaga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt tgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttctttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaa
//
LOCUS       DADLZM010000125        11555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLZM010000125 DADLZM010000000
VERSION     DADLZM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.126"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JNY73_003223"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JNY73_003223"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNY73_003224"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNY73_003224"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY8049135.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNY73_003225"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNY73_003225"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY8049136.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNY73_003226"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNY73_003226"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY8049137.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /locus_tag="JNY73_003227"
     CDS             complement(3367..4701)
                     /locus_tag="JNY73_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8049138.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDSAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="JNY73_003228"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="JNY73_003228"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY8049139.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPTSELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNY73_003229"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNY73_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY8049140.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="JNY73_003230"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="JNY73_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="with KbaY catalyzes the formation of
                     dihydroxyacetone phosphate and
                     D-glyceraldehyde-3-phosphate from
                     tagatose-1,6-bisphosphate; subunit Z may act as a
                     chaperone for the Y subunit; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="JNY73_003231"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="JNY73_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY8049141.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="JNY73_003232"
     CDS             9047..9826
                     /locus_tag="JNY73_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY8049142.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="JNY73_003233"
     CDS             9816..10625
                     /locus_tag="JNY73_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY8049143.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..11146
                     /locus_tag="JNY73_003234"
     CDS             10712..11146
                     /locus_tag="JNY73_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8049144.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11143..>11555
                     /locus_tag="JNY73_003235"
     CDS             11143..>11555
                     /locus_tag="JNY73_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY8049145.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2755 a         3101 c         2949 g         2750 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggcgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ccaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg ctgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaagtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccga cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactacataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactcgaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcaggtcag aaaactatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtatcaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cctttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg
    10921 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac
    10981 cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga
    11041 agaattccgt gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga
    11101 gttaggccgc tgccatgaag aatatccggt cgaggaagga atatgacaca cgttctgcgc
    11161 gccagaaggc tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac
    11221 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    11281 ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat
    11341 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    11401 tggttaccga ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt
    11461 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    11521 ccgtacttca cgccgcagaa taaaggcgcg catcc
//
LOCUS       DADLZM010000126        11450 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLZM010000126 DADLZM010000000
VERSION     DADLZM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.127"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..155)
                     /locus_tag="JNY73_003236"
     CDS             complement(<1..155)
                     /locus_tag="JNY73_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049146.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKSCRLAP"
     gene            336..1967
                     /gene="dcuS"
                     /locus_tag="JNY73_003237"
     CDS             336..1967
                     /gene="dcuS"
                     /locus_tag="JNY73_003237"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY8049147.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1964..2683
                     /gene="dcuR"
                     /locus_tag="JNY73_003238"
     CDS             1964..2683
                     /gene="dcuR"
                     /locus_tag="JNY73_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY8049148.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3254..4594
                     /locus_tag="JNY73_003239"
     CDS             3254..4594
                     /locus_tag="JNY73_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8049149.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4672..6318
                     /locus_tag="JNY73_003240"
     CDS             4672..6318
                     /locus_tag="JNY73_003240"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY8049150.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6441..7070
                     /locus_tag="JNY73_003241"
     CDS             6441..7070
                     /locus_tag="JNY73_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY8049151.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7209..8618)
                     /gene="melB"
                     /locus_tag="JNY73_003242"
     CDS             complement(7209..8618)
                     /gene="melB"
                     /locus_tag="JNY73_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY8049152.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(8733..10088)
                     /gene="melA"
                     /locus_tag="JNY73_003243"
     CDS             complement(8733..10088)
                     /gene="melA"
                     /locus_tag="JNY73_003243"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY8049153.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10371..11279
                     /gene="melR"
                     /locus_tag="JNY73_003244"
     CDS             10371..11279
                     /gene="melR"
                     /locus_tag="JNY73_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY8049154.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         3006 a         2941 c         2768 g         2735 t
ORIGIN      
        1 ggggccagtc tacacgactt tcgtttgaga ttaaataact tgccgttgcc acgtacgcaa
       61 ttgccagaat gctgacatat cgcagtgttg aaatagacat cgatcttttc gccggtataa
      121 cagcgataac ccccgtccag tagcgcctga tccatgacct tccctctctc atcagtatga
      181 ttatcgctat aagcatagcc ccggaatctt catacagcac tggcagtttc cgttgccaga
      241 gtctcttccc ctatatagcc aatcattttt actccctttg aattacccgc ctcatcagag
      301 ataatgctta agaaatttgt cacacaagga agctgatgag acattcattg ccctaccaca
      361 tattacgcaa acgtccgatg aaattgagta ccactgtgat cttaatggtc agcgcggtac
      421 tgttctcggt gctattggtg gtgcatctga tttacttctc gcaaatcagt gatatgacgc
      481 gagacgggct ggccaacaag gcactggcag tggcgcgtac cctcgccgac tcgccggaaa
      541 tccgtcaggg cttgcagaaa aaaccgcagg agagtggcat ccaggccatc gcggaagccg
      601 tgcgcaaacg caacgatctg ctgtttattg tcgttaccga tatgcaaagt cttcgctact
      661 cacatcctga agcccagcgt attggtcagc catttaaagg tgatgacatc cttaatgcgc
      721 tgaatggcga agaaaatgtc gctatcaatc gcggttttct ggcgcaggct ttacgcgtat
      781 ttacccccat ctacgatgaa aatcataaac aaattggcgt ggtggcgatc ggccttgagt
      841 taagccgcgt gacccaacag atcaatgaca gtcgctggag cattatctgg tcggtattat
      901 ttggcatgct ggtcggactg attggcacct gcattctggt taaggtactg aaaaaaatcc
      961 ttttcggcct ggaaccctac gaaatctcca cgctgtttga gcaacgccag gccatgttgc
     1021 agtctatcaa agaaggcgtc gttgccgtgg acgatcgcgg cgaggtcacg ctgatcaacg
     1081 atgccgcaca agaattgctg aattaccgta agtcgcagga cgatgagaaa ctgtcgacgc
     1141 tcagccactc atggtcacag gtggtagatg tctcggaagt gttacgcgac ggtacgccgc
     1201 gccgcgacga agagattacg attaaagacc ggctattact gatcaacacc gttccggtgc
     1261 gcagtaatgg cgttatcatc ggtgccattt caaccttcag ggacaaaact gaagttcgta
     1321 aactgatgca gcgactcgac ggtctggtca actatgctga cgcacttcgt gaacgatccc
     1381 acgaatttat gaataaattg catgtgattc tcggattatt gcatctgaag agttataagc
     1441 agttggaaga ttacattctc aaaacagcca ataactatca ggaagagatt ggctctctgc
     1501 tgggtaagat caaatctccg gttatcgctg gttttttaat cagcaagatt aaccgcgcga
     1561 ccgatttagg ccatacgctg attttaaaca gtgaaagcca gctgccagac agcggcagtg
     1621 aggaccaggt cgcgacgctg attaccacat tgggaaatct gatagaaaac gcgctggaag
     1681 cattagggcc ggaacccggt ggcgaaatta gcgtaacatt gcactaccgt cacggctggc
     1741 tgcactgcga agttaacgat gatggaccgg ggatcgcacc cgacaaaatc gatcacattt
     1801 ttgacaaagg tgtctcgaca aaaggaagcg agcgaggcgt cggtttagca cttgtcaaac
     1861 aacaggtaga aaatctcggc ggcagcatcg ccgtggaatc ggaaccgggg attttcacac
     1921 aattttttgt ccagataccc tgggacgggg agaggtcgaa cagatgatca atgtattaat
     1981 tatcgatgac gacgcaatgg tcgcggagct gaatcgccga tacgtagcac aaatcccagg
     2041 ctttcaatgc tgtggaacag cctcgacgct ggagaaagcc aaagagatta tcttcaatag
     2101 cgatgcgcct atcgacctga tattgctcga tatctatatg caaaaagaga acgggctcga
     2161 tttactgcct gtcctgcata acgcgcgttg caaaagtgat gtgattgtca tctcctccgc
     2221 agccgatgcg gcaaccatta aagattcgct gcattacggt gtcgtggatt acctgatcaa
     2281 acccttccag gcttcccgct ttgaagaggc gctcaccggc tggcggcaaa agaaaatggc
     2341 gctggaaaaa catcagtatt atgaccaggc cgagcttgat cagctaatcc acggcagcag
     2401 ctccaacgaa caggatcctc gtcgcttgcc aaaaggctta acgccgcaga cgttacgcac
     2461 gctgtgccag tggattgacg cgcatcagga ctatgacttt tcaaccgacg agctggctaa
     2521 cgaggttaac atctcgcgtg tttcctgccg aaaatacctc atctggctgg tcaactgcca
     2581 catcttgttc accagtatcc attatggcgt cacggggcgt ccggtttacc gttaccgtat
     2641 tcaggcagaa cactactcac tgctgaaaca atattgccaa taacggatcg ctggttatct
     2701 gtaagtaata attattaccg taatgcaaaa tacccctggc acaagttaca tcgttaatca
     2761 gtaaaacttg tgccagatca aatataatta tccctccatc atcgttaaaa attaatatct
     2821 cttcagatga acggtgtttt taatttcaaa acgataacaa aagttaatta actattatgt
     2881 cacccgcatt atgtgtattt ttacccacaa atgggtagat cagattaatc tataaaccta
     2941 atgatatctg ccctgagaac aaaaaataga ccgataaata tcaataagat aacagcaaac
     3001 aaaacattaa catctgcgca gtacaaacta taaacccatc gccagagagt ttttctctct
     3061 gaaaaagccg cttatcacag tgcataaatt tgccgctgct ttaatcagcc aatattcact
     3121 gtgaggtatt tgctaaagcc ggtaacgacc aaacggatat ttagtcaggc tctgaaaaca
     3181 gttcatacaa aacagaacgt gactgtgatc tattcagcaa aaatttaaat aggattatcg
     3241 cgagggttca cacatgttat ttactatcca acttatcata atactaatat gtctgtttta
     3301 tggtgccaga aagggtggta tcgcgctggg tttattaggc ggtatcggtc tggtcattct
     3361 ggtcttcgtc ttccaccttc agctaggtaa accaccagtt gatgtcatgc tggttatcat
     3421 tgcggtggtg gcggcatcgg cgaccttgca agcttcgggc ggtcttgatg tcatgctgca
     3481 aattgccgag aagctgctgc gccgcaaccc gaaatatgtc tcaattgtcg cgccgtttgt
     3541 gacctgtaca ctgaccattc tttgcggtac gggtcatgtg gtttacacca ttctgccgat
     3601 catctacgac gtcgccatta agaacaacat ccgtccggaa cgtccgatgg cggcaagttc
     3661 tatcggtgca cagatgggga ttatcgccag tccggtgtcg gttgcggtcg tgtctctggt
     3721 tgcgatgctg ggtaatgtca cctttgatgg tcgccatctt gagttcctcg atctgctggc
     3781 aatcaccatt ccatcgacgt taatcggtat cctggcgatc ggtatcttca gctggttccg
     3841 cggtaaagat ctggataaag acgaagagtt ccagaaattc atctccgtac cggaaaaccg
     3901 tgagtatgtt tacggtgata ccgcgacgct gctggataaa aaactgccga aaagcaactg
     3961 gctggcaatg tggattttcc tcggggcaat cgctgtagtc gcccttcttg gtgctgattc
     4021 ggacctgcgt ccatccttcg gcggcaaacc gctgtcgatg gtactggtta ttcagatgtt
     4081 tatgctgctg accggggcgc tgattattat cctgaccaaa accaatcccg cgtctatctc
     4141 aaaaaacgaa gtcttccgtt ccggtatgat cgccatcgtg gcggtgtacg gtatcgcatg
     4201 gatggcagaa accatgttcg gtgcgcatat gtctgaaatt cagggcgtac tgggtgaaat
     4261 ggtgaaagag tatccgtggg cgtatgccat tgttctgctg ctggtttcca agtttgtaaa
     4321 ctctcaggct gcggcgctgg cggcgattgt tccggtcgcg ctggcgatcg gcgttgatcc
     4381 ggcatacatc gtggcttcag caccggcttg ctacggttat tacatcctgc cgacttatcc
     4441 gagcgatctg gcagcgattc agtttgaccg ttccggcacc acccacatcg gtcgcttcgt
     4501 catcaaccac agttttattc tgccggggtt gattggtgtg agcgtatcgt gcgtcttcgg
     4561 ctggatcttc gccgcgatgt acgggttctt ataaatgcac tttgcgtgcc gcccgtgact
     4621 acgcggcacg ccattttcga ataacaaata cagagttaca ggctggaagc tatgtcaaac
     4681 aaacccttta tctaccaggc acctttcccg atggggaaag acaataccga atactatcta
     4741 ctcacttccg attacgttag cgttgccgac ttcgacggcg aaaccatcct gaaagtggaa
     4801 ccagaagccc tgaccctgct ggcgcagcaa gcctttcacg acgcttcttt catgctccgt
     4861 ccggcacacc agaaacaggt tgcggctatt cttcacgatc cagaagccag cgaaaacgac
     4921 aagtacgtgg cactgcaatt cttaagaaac tccgaaatcg ccgccaaagg cgtgctgccg
     4981 acctgccagg ataccggcac cgcgatcatc gtcggtaaaa aaggccagcg cgtgtggacc
     5041 ggtggcggtg atgaagaagc gctgtcgaaa ggcgtctata acacctatat cgaagataac
     5101 ctgcgctact cgcaaaacgc ggcgctggac atgtacaaag aggtcaacac cggcactaac
     5161 ctgcctgcgc aaatcgacct gtacgcggta gatggcgatg agtacaaatt cctttgcgtt
     5221 gcgaaaggcg gcggctctgc caacaaaacg tatctctacc aggaaaccaa agccctgctg
     5281 actcccggca aactgaaaaa cttcctcgtc gagaaaatgc gtaccctcgg tactgcagcc
     5341 tgcccgccgt accatatcgc gtttgtgatt ggcggtacgt ctgcggaaac caacctgaaa
     5401 accgtcaaat tagcaagcgc tcactattac gatgaactgc cgacggaagg gaacgaacat
     5461 ggtcaggcgt tccgcgatgt ccagctggaa caggaactgc tggaagaggc ccagaaactc
     5521 ggtcttggcg cgcagtttgg cggtaaatac ttcgcgcacg acattcgcgt tatccgtctg
     5581 ccacgtcacg gcgcatcctg cccggtaggc atgggcgtct cctgctccgc tgaccgtaac
     5641 attaaagcga aaatcaaccg cgaaggtatc tggatcgaaa aactggaaca caatctaggt
     5701 cagtacattc cacaagaact gcgtcaggcc ggtgaaggcg aagcggtgaa ggtcgacctt
     5761 aaccgcccga tgaaagagat cctcgcccag ctttcgcaat atccggtttc cactcgtttg
     5821 tcgctcactg gcaccattat tgtgggtcga gatattgcac acgccaagct gaaagagctg
     5881 attgacgccg gtaaagaact tccgcagtac atcaaagatc acccgatcta ctacgcgggt
     5941 ccggcgaaaa cccctgccgg ttatccatca ggttcacttg gcccaaccac cgcaggtcgt
     6001 atggactcct atgtggatct gctgcaatcc cacggcggca gcatgatcat gctggcgaaa
     6061 ggcaaccgca gccagcaggt taccgacgcg tgtcataaac acggcggctt ctacctcggt
     6121 agcatcggcg gtccggcggc ggtactggcg cagcagagca tcaagcatct ggaatgcgtc
     6181 gcttatccgg agctgggtat ggaagctatc tggaaaattg aagtagaaga tttcccggcg
     6241 tttatcctgg tcgatgacaa aggtaacgac ttcttccagc aaatcgtcaa caaacagtgc
     6301 gcgaactgca ctaagtaacc tcttcggccc agcacctggc agcatgctgc caggtgatcc
     6361 ccctgggcca cctcttttgc gattgtaatt tcatgcttgc tggtgaatag tcagtatttt
     6421 ccctgacttg cgaactcacc atgacccgca cactcaagcc gttaattctt aacaccggcg
     6481 cactggcgct aacgttaatc ctgatttata ccggcatttc ggcccatgac aaactcacct
     6541 ggctgctgga agtgacaccg gtgattatcg tcgtgccgct actacttgcc accgccagac
     6601 gttacccgtt aacgctgctg ctctatacgc tcatcttctt tcacgccatc atcctgatgg
     6661 taggcgggca gtacacctac gcgaaagtcc ccgttggttt tgaggtgcag gaatggctgg
     6721 ggttgagtcg taatccgtat gacaagctgg ggcacttttt ccaggggctg gtgcctgcac
     6781 tggtggcacg agaaattctc gttcgcggga tgtacgtgcg cggacgtaaa atggtggcgt
     6841 ttctggtctg ttgcgtggcg ctggcgataa gcgcgatgta tgaattaatc gagtggtggg
     6901 cggcactggc gatgggccag ggagcggatg attttctcgg tacccagggc gaccagtggg
     6961 acacgcaatc cgatatgttc tgcgcgctgc ttggcgcatt aacgacagtg atattgctcg
     7021 ctcgctttca ttgccgccag ctacggcgct atggcttgat aaccggataa caacgtgcac
     7081 tttgttgatg ccggatgcgg cgtgaattcc ttatccggtc tacaaaattt gcaaatccaa
     7141 agcattacat taagacgtgg cctgataaac atatcgcatc aggccatttt cgttagctat
     7201 tgtccacgtc aggctttcac atcactcact gcaccgaccg gaatatcagc atgaacaggc
     7261 tcgggcggta ctttgcgata tttatccagc agatgaatct ggatcctgcg cagcgtgtcg
     7321 ccattgaggc gatagaaacg gaagtagaga atcagcgtta ccatgaaaaa cagagtcggt
     7381 agagcaatca taataaactg catacctaat agggcttgcg tagactgttc aacattcggt
     7441 acatagccaa tcatccctaa cacaaccgca atgaaaaaag ccgcaaaggc tgaaccgccc
     7501 ttcaccacca tagtctgcac ggagtaagcg atgctttcac agcgtacgtg cagtttatat
     7561 tcaccgtaat caacggtatc tgccaccatg atgacctgta atacccagaa aagcgccgtt
     7621 cccacattca gcaaaatacc cgcaatcaca atgaggacga cgttgtgata gctcattaat
     7681 gccattaaca ggagaacacc acagcttaac accggaagaa tagatgctcc ggcccataaa
     7741 atgcgtcggg ataatgattt aactaagcgt gggaagaata ctaacgtcac caggttagca
     7801 gctcccgcat acgacagata ataggggaac aaatccgcat caccgataac atatgagaaa
     7861 taatagatag caaatccggt aataatgttg ctggctacat tataagcaag agccataccc
     7921 aagaggcatg aaagctgatc gtttttataa ataagcgcaa cgatggcttt aagtgtcaga
     7981 tggcttcctt cagcagacga ttgattgtct gacgaaaaga cttcatgcac attgcgcaga
     8041 gtgatgatgg ttgaaacaat aaaaaaggcg atcaatacca gagtgaacat ctgaaagcca
     8101 aatccccgat cgccaccgcc gacataatta acaaatggta gcgtcacacc tgccgtaaca
     8161 aagcctgcca gactggcaaa aaaacgcgga taaggaacca gttgttcgcg ttcacgttta
     8221 tcgagcgtga tggttggaac cagcgaccag aagggaatat ccataatggt gtaagtcatg
     8281 ccccagagga tgtaggtcac gcaaacaaag acaatctgag tagtaccttc aaacagatgc
     8341 gcactaaaga ggagaaataa gattacagag tttgccaacg taccgatcag gatccagggt
     8401 ttgaacttac cccatcgcga tcgcgtagca tttacaatcc atcccataat cggatcgtta
     8461 atagcatccc agatcctcgc caccagaaac aaagtaccga ccaaacccac agacagcccg
     8521 acgacatcgg tgtagtaata catgaggtac atatacacaa tgccgatcgc aaaatccttc
     8581 ccgaacgctc caaatccata actgagtttt gtagtcattg aaatgctcat agggtatcgg
     8641 gtcgctgtta ccaacgcccg ccttctgtta ttggtatcgt cagaggtgtg cgccggaata
     8701 aatcccccgg cgcagtagcg tttagtcgcg ttttaacggt gcaaccagcc tggcagccag
     8761 tcgccgtggg cggcaatcag gtcgtcaaca agagcatata tttcgtcaat gcccagcacg
     8821 gcggcagtat gcgggtccat catcgcggcg tggtaaacac ggtcgcgatt ttccgtaaga
     8881 atagcttcgg tcagcagcgt ctgtacgttg atgttggttt gcatcagggc ggccagatgc
     8941 gaaggtagcg taccgacttt ggtcggctga atgccattag catcaaccag acaggctact
     9001 tccacgcaac atccttgtgg caggttatca atcaaaccat cgttacggac gttgccgtaa
     9061 atcacactcg gctcgccagt ccagatagcg ttcatgattg tgctggcata ttcccgtgac
     9121 ggtttaatat caatccggga ggcgttttta tactcctcca gctctttatg ccagttcgcc
     9181 agctgctcga cgcagcgttt cgggtactca tccagcggta ctttataacg ctcaatcaaa
     9241 tcctcacgac ctagcttaat aaaccacggc gtatattcgg caaaatgttc tgacgattcc
     9301 gtgacgaagt agcccagctt tttgaacatt tcgtagcgca caatattctg gcagcgagta
     9361 ttaccatgaa tatttggctt cggtgcctgc cctgcttcat aagccgccag cagttccggg
     9421 tagagattca cgtaactgcc gtcggcggtt ttgcgctcca gctccaggta aaacgccata
     9481 tggttgatac ctgcgcaacg gtaacgcagc gtagccgggt cgatattgag gtcacgcgcc
     9541 agctcttccg ccgttccctg caccgaatgg cacagcccga cctgtttgat atacggatag
     9601 cgggcataca tcgcccaggt attcatcgcc attgggttaa catagttgag catggtggca
     9661 tcggggcaga cttccgtcat gttctcgcaa atttgccaca gatgcggaat ggtacgtagc
     9721 gcgcgcataa taccgcccgg ccccaacgta tcggcaatgg tttgttccag accatgccgc
     9781 ttacagacct cgaaatcagt caccgtgcaa ggttcataac cgccaatctg aaatgccacc
     9841 acgacaaaat cggcatcctc taaggcttct ttctgttggg tgtggcaggt gattttgccg
     9901 ctggcccctg ctgaatccat cagcttacgc accacaatat gcgactcttc caggcgggtg
     9961 gggtcaatgt ccatcagggc aatatgcgcc gttttcagcg cctcgcgatg gaacacatca
    10021 ccaagaatat ttttaacgaa aatcgtcgaa ccagcgccga taaatgtaat tttgggtgca
    10081 gacatcatgc agatctcctg gcttgcttga ataacttcat catggcaggc gaatctgcag
    10141 aaaacctccg gcttccaggt tgagcattcc cataaactca gatcctgcgt gaagcagcag
    10201 taaatctgag tttatgggaa taaattacct gaaaagtaga gttttcggcc tcatgatgct
    10261 cggacagtcc cctcgcccct ctgggaagag ggtgaaaacc gtgctcccac tcgcagtcat
    10321 cctccctcac tcctgccata attctgatat tctaggaaag agagccatcc atgaatacag
    10381 atacgtttat gtgcagcagc gacgaaaaac agacccgcag cccgctgtcg ctgtattcgg
    10441 aataccagcg gatggagatt gagtttcgcg caccgcatat catgcccacc agccactggc
    10501 atggtcaggt cgaagtgaat gtgcctttcg atggcgatgt ggaatacctg atcaacaatg
    10561 aaaaagtgaa tatcaatcag ggtcatatca cgctgttctg ggcctgtaca ccgcaccaac
    10621 taacagatac cggaacctgt cagagcatgg cgatttttaa tctgccgatg catctgtttc
    10681 tctcctggcc gctggataaa gacctgatta accacgtcac tcacggcatg gtgatcaaat
    10741 cacttgccac acagcaactt agcccgtttg aagtacgccg ctggcagcag gaattaaaca
    10801 gtccgaatga gcaaattcgc cagctcgcca ttgatgaaat tggcctgatg ctcaagcgat
    10861 ttagcctctc tggctgggaa ccgattctgg tcaataaaac ctcgcgcaca cacaaaaaca
    10921 gcgtctcgcg ccatgcgcaa ttttatgtta gccagatgct gggctttatt gccgaaaact
    10981 atgatcaggc gctgaccatc aacgatgtgg ctgagcacgt caaacttaac gccaactatg
    11041 caatggggat attccagcgg gttatgcaat tgacgatgaa acagtacatt accgcgatgc
    11101 gcatcaacca cgttcgcgcg ttactgagcg ataccgataa aagtattctc gatattgccc
    11161 tgacggcagg ctttcgttca agtagtcgtt tttacagcac gttcggcaaa tatgtcggca
    11221 tgtcgccgca acaataccgc aaacttagcc aacagcgccg ccagacgttt cccggctaaa
    11281 gacgatatca gtatcagcca aaaaaatagt ttagccgtcg ataataagca gtgaaaataa
    11341 tttaaaataa tcacataacg tattgtttat aaaactaaat atttcaaaaa tgtttgtttt
    11401 tcacgcgctt tacagcccga aaaggccgga agatacttgc ccgcaacgaa
//
LOCUS       DADLZM010000127        11440 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLZM010000127 DADLZM010000000
VERSION     DADLZM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.128"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            9..461
                     /locus_tag="JNY73_003245"
     CDS             9..461
                     /locus_tag="JNY73_003245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8049155.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="JNY73_003246"
                     /pseudo
     CDS             707..1751
                     /locus_tag="JNY73_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1867..2040
                     /locus_tag="JNY73_003247"
     CDS             1867..2040
                     /locus_tag="JNY73_003247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049156.1"
                     /translation="MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDN
                     AWALSESWCGTIQ"
     gene            2089..2427
                     /locus_tag="JNY73_003248"
                     /pseudo
     CDS             2089..2427
                     /locus_tag="JNY73_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JNY73_003249"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JNY73_003249"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY8049157.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="JNY73_003250"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="JNY73_003250"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY8049158.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="JNY73_003251"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="JNY73_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY8049159.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /locus_tag="JNY73_003252"
     CDS             6055..6456
                     /locus_tag="JNY73_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY8049160.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JNY73_003253"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JNY73_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY8049161.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="JNY73_003254"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="JNY73_003254"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY8049162.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="JNY73_003255"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="JNY73_003255"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY8049163.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10321..10396
                     /locus_tag="JNY73_003256"
     tRNA            10321..10396
                     /locus_tag="JNY73_003256"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10354..10356,aa:Thr,seq:cgt)
     gene            complement(10411..>11440)
                     /locus_tag="JNY73_003257"
     CDS             complement(10411..>11440)
                     /locus_tag="JNY73_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8049164.1"
                     /translation="AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWML
                     HFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTK
                     TRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPV
                     VMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQ
                     GLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWA
                     SWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEE
                     NVVYLNAR"
BASE COUNT         2746 a         2838 c         2946 g         2910 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggtccat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtag
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcgtagctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gtgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtttggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat taccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctgaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact
    10381 cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc
    10441 ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc
    10501 ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc
    10561 tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt
    10621 atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca
    10681 tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg
    10741 gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa
    10801 tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc
    10861 agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac
    10921 gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc
    10981 aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt
    11041 aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt
    11101 gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg
    11161 tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt
    11221 caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat
    11281 gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc
    11341 atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag
    11401 ctttttcggt gattccccga gattttttac tctccacgct
//
LOCUS       DADLZM010000128        11381 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLZM010000128 DADLZM010000000
VERSION     DADLZM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.129"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(379..942)
                     /locus_tag="JNY73_003258"
     CDS             complement(379..942)
                     /locus_tag="JNY73_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8049165.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(989..2350)
                     /locus_tag="JNY73_003259"
     CDS             complement(989..2350)
                     /locus_tag="JNY73_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HAY8049166.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2402..2632)
                     /locus_tag="JNY73_003260"
     CDS             complement(2402..2632)
                     /locus_tag="JNY73_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049167.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(3670..3861)
                     /locus_tag="JNY73_003261"
     CDS             complement(3670..3861)
                     /locus_tag="JNY73_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049168.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(3858..4280)
                     /locus_tag="JNY73_003262"
     CDS             complement(3858..4280)
                     /locus_tag="JNY73_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY8049169.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4327..4752)
                     /locus_tag="JNY73_003263"
     CDS             complement(4327..4752)
                     /locus_tag="JNY73_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY8049170.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(4725..5297)
                     /locus_tag="JNY73_003264"
     CDS             complement(4725..5297)
                     /locus_tag="JNY73_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YubH family protein"
                     /protein_id="HAY8049171.1"
                     /translation="MCCVYRMNRPAAGLTVVFCGRWSGKPGPKSAAWRMPWQKSGNDD
                     VGGVNPRLFPAGPRTEHKGSRRRLRFTRPCRAWPCGFSVMWPQRRVRAVPCLHLSRAG
                     RDARVRFAAAVTRSLLPVCRDFPVVHPLRFRGLTLQLPPAVCVRLRLPLRPVHPRLIA
                     RLLWRHGTARCRGICEQRRKTSCNMRNLSL"
     gene            complement(5721..5946)
                     /locus_tag="JNY73_003265"
                     /pseudo
     CDS             complement(5721..5946)
                     /locus_tag="JNY73_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5997..6431)
                     /locus_tag="JNY73_003266"
     CDS             complement(5997..6431)
                     /locus_tag="JNY73_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY8049172.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(6445..6666)
                     /locus_tag="JNY73_003267"
     CDS             complement(6445..6666)
                     /locus_tag="JNY73_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049173.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(6667..7350)
                     /locus_tag="JNY73_003268"
     CDS             complement(6667..7350)
                     /locus_tag="JNY73_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY8049174.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(7427..7720)
                     /locus_tag="JNY73_003269"
     CDS             complement(7427..7720)
                     /locus_tag="JNY73_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049175.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            7868..8830
                     /gene="parM/stbA"
                     /locus_tag="JNY73_003270"
     CDS             7868..8830
                     /gene="parM/stbA"
                     /locus_tag="JNY73_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HAY8049176.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8833..9183
                     /locus_tag="JNY73_003271"
     CDS             8833..9183
                     /locus_tag="JNY73_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein stbB"
                     /protein_id="HAY8049177.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(9306..9587)
                     /locus_tag="JNY73_003272"
     CDS             complement(9306..9587)
                     /locus_tag="JNY73_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049178.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(9715..9949)
                     /locus_tag="JNY73_003273"
                     /pseudo
     CDS             complement(9715..9949)
                     /locus_tag="JNY73_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752033.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10885..>11381)
                     /locus_tag="JNY73_003274"
     CDS             complement(10885..>11381)
                     /locus_tag="JNY73_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049179.1"
                     /translation="QGLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSD
                     TKATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKNIDNYFLFIKQKIESFNFNEAL
                     KQQILKDISLCSYEGLHVLKIEIKSVGEVCHFENRYYIRQGTATHLITDGEAISSLHR
                     LYYS"
BASE COUNT         2774 a         3081 c         2835 g         2691 t
ORIGIN      
        1 tccggtcgct gcaaacccgg gtttttgcgt cagggatgga agcccgccag ggcggagaca
       61 gctccgctgg ctccgttcac gacagcccgc cccgccaggg ggacgcccgg aaaaggcata
      121 agcaggagaa caacaaaaca gacagtaatc agagcagcag aaaaaagaag aacagtagca
      181 gtagcagtag cagtagcagg aacaacaaca tcatcagtaa caggcaaatg acaggtctga
      241 aaagacagaa gcccacagca gaacgtccgc gtaatgaaag gggcggcgcg taagcgcggc
      301 ccctttcagc gccaccggcg cgcggtcttt tcccggcgcg ggcagacggg catttatcct
      361 cccgcctgcc cggattcatc aggcacgcag acgaataatc atcgtcggaa cacgggtata
      421 agcaaatgtg ccgcacggca gcacctcgcg gacatcagaa aacggtaaca gcttctcctg
      481 ctggcgcggc ccgttcagac agacggcaac cagaacgccc cccggacgca acagggaaaa
      541 agcccgcagg atatgccgga tatcctgccc gtgactgaac ggcggattca taatgatccg
      601 gctgtaatac tgcaccggct gccattccat gaagtcgcca cactgtaccc tgacaccgtt
      661 aaagttttcc cgcagatacc ggaccagccc gctgttaatc tccaccgcat cacacatggc
      721 ctccggcgcg gtatcccgaa tcgcccgcag tatggccccg gtgccggcag agggttccag
      781 aatgtgatca cggttgctga tttccgccag cgtcaccagg cgatcacaga ccggggcagg
      841 agtcacaaaa agctgagatt cagcccggat ttcagcaacc ggcgcggcgt tatccagcct
      901 tgcgcgcatc agccggatac gttctcttaa ctgactgtgc atgtcattat ctccgtcatc
      961 ccccggggat ttcccggggg gatatacctt atttctgcgg gatttccgtg attttcatgt
     1021 cggtgatata cacattcacc agacggaaat tattgtccat tgtgcggcgg tagcggtatg
     1081 ccccgtggtc ttctgcttct tccacactgc gcacggcctt acagtcccgg ggcagggcgg
     1141 cccactgcgc ctttgtcatt tcccggaaac cttcccccgg atagttgact accggcggac
     1201 gcttcgcggc ctgactggcg acggcagcct cttctgccga tggtgctttg tagtccgtga
     1261 tgcgatcggg cgtcactttc atcgtgccgc tgtacccgag aaaactgtaa tttggcgttg
     1321 tgactgaact caccgccccg ttgcttttgt tcacgcggat gatagtcagc cactcgcccc
     1381 ggctgaaaac ctgtccgccc ggctcaaaat cctgtgtccg ggtaaccacg ccgccgcttt
     1441 cgtccagcat ggcacgctca tagatcaggc ggttctgata gtggttaacc cagcgttgct
     1501 gatgctgaat ctgccgttca tgacagcgga tcgcaatttc gcgggcctgc tccgtggtga
     1561 tgatgtcatc atccagggcc gaccataacg accggctccc ttcatactgg cttttttctg
     1621 ccgggcgcgg atacgtatcc agcgggaaac aggcgctgat atggtcatga ctgctgatga
     1681 gttttgccat attcagatcc agcgattcag cccgccacat cgtcagatat ttctgcgact
     1741 gtgcgatggt cttttcagcc ttgcgcaaat ccgcttcgat ttttttgata cgacgccagc
     1801 gaacgtccgg gcgttcttta tacttcgcgt gaagcagtgc cgaccgcgcc cgctcctccc
     1861 agtattccgc acgttcaaag agcatcacgg cacgtttcat gccgttttca atacgctgcg
     1921 catcacgacg ggcgcggcgt tcgctgtgat gccccacaag aatgggctga ccgggcggga
     1981 tcatcgcggc cagtctttcc acttcatcaa gtgcctgtgc ggattcactg gcccgctttc
     2041 cgctgtatcc ggtaaaccgc tccgcccgtg cttcctgacg ttcagcgagc gtgctgtctt
     2101 catcctcaat ctctccggca agtgagagga gcacgtcttc ccggcccggt gtccaggctg
     2161 gcgcgacaaa cagcgcctgt tttggtgccc agcgaaaacc agcatcatgc acttttttat
     2221 acgtctccgg gtcaagacgt gacacggcat acaggcgcag tttgttgtca tccggtgagt
     2281 aggtcgcgcg gtacatctgc acagggctgg cggcttccgg ggcggtcatg gtgattgctg
     2341 ctaatgtcat aaaatgttct ccttccgtct gttgccgccc ctcgcggggc ggctcacctc
     2401 atcagttaaa agcgcgtttc agttcgagtg tgcggagcac ctgcccgaaa gccgtgtttt
     2461 tcatggcggg gatgtgtaca ccacgccccc ggtgaaaact ccgggctatg tgtcgcccga
     2521 aggctttcaa cacggggtca atttccggcg tggggatgtc ttcgcgtctg aaacggggat
     2581 gacggcgggg ctttacagcc gctgcggaag gtgaaacaat gcttacagtc ataaatggat
     2641 tccttatgac aaattggtta acaatgccgt caaaattggt cgcacaattc ttacggcgtg
     2701 gattaagcag gtgcctgacc tgcttttttc atatctgcat ctgacgcttt ccggtgtttt
     2761 cctccttctg ttcctgcgtt ctcattacct gtcagccgga tgactgacag aacaaataaa
     2821 tgcgtattgc cggagcctga aataaatcct gatttttaaa gaacatcacc cgttgagttt
     2881 cgggagccgc ggttttgacg tttcgcggga acctgctgct gtattcactg tggtatgaac
     2941 ttcacccgtt tgagaacctt cgccgcaagg ggcatcactg ccacggcctg acgcacctgt
     3001 cctgggcaga acgcaaaatt ttttaccggg ttttgggtaa aaaattttgt gggctgttca
     3061 tttaccctgg acagtcagtg cggcaggttg tggctgtatc agcccccggc gacagggact
     3121 catacgggtg tgtggcaccc ggcaggcaat gagcaggggc ccgccgcaac gacggagagc
     3181 gcgtgcgcga aacggcgttg ccccttacat cccggcggcc ttttgccggg atgccgggag
     3241 cgcaggccac ggcgcgacag aggcggcgcg accggaacgg tcgctgcaag ccaggggttt
     3301 tcacgcatcg gaccgtagcg gcagcccgaa accgcgccag cgggttcggc ggagactggc
     3361 aggtgcgcca gcagctgcct gtcggagtcg ggcctgacga ccggatgccg ccaggcagcc
     3421 gggagccgaa ggtgcgcccg gcagacagaa caacactgca gtagtcagaa tcagagcagt
     3481 agataaagga acaacaacat caggaacagt tccataacat aagaaagaaa cactcctgag
     3541 tcagacacat cacagacggg aagggtaacg ggcgacgcgc cagcgtggcc cgcttccctt
     3601 ccggcaggcg tcagcctgcc aggggttttc gcgcgggctg acaggcggcc tccaccgcct
     3661 gcccctgtat cacctgttta tgctgccctg aaggatgtag tgaccgccga ggaatacagc
     3721 ctgctgattc gccacaaaat cgcagatttc atcaacaatc tgcgcagtgg tgagcggttt
     3781 ctgccccatt tcacgacgcc gcgcattaat cacggctttg taattgcgca gaatttcgcg
     3841 ggtttctgtg ctgatattca tgacatgtgc tccccgtcca gcgcctgtaa cacaacgtcc
     3901 attgcttcgc gttcttccct gacgaatgcg tcaccgtcgc ccccgttctc gctcccgacg
     3961 gcatacaggc gcttcatttc actgcctgcc agcgccatca ctttttccag catcaccgcc
     4021 ggaactgtca cctgacggct ggcgcgcttt tcttccatca gctcacgaac aacgtcgtga
     4081 acaatgctga catccgcacc atgcgcaaag gcatcgtcca gtcgctgcat gacggtttcc
     4141 agctcatcgt ctgtcagact ccagtcctgg gctatgtgac gcacatcccc acgggtcagc
     4201 accacggcaa aaacgcattc actgtccggc acgtctgtgt tcagcacttc gcggataatt
     4261 tctttaacag tacagtacat gttctcgtct cctttacagg ggcggcattg ccgccccgtc
     4321 cgttaatcag tccagtgccg cgtaaatcgc gttgcattcg gggtgaaatt cgatgtgacg
     4381 ccacaactgc gcatcgcgca tccggtaaag ctgggtcagt cctgcatccc cgctgtcgtg
     4441 gtacagccac aactggcggt taatcacaag ggaagtcaga atgatgcctg cggcatcagc
     4501 actcacggta cggtcaaacc agtttgcacc gttcaccatg tggaaacggt cgccatccgg
     4561 catcatgtaa ccgccaccgt ccggcaggcg gatgaagtgc cagaagcctc cctgatattc
     4621 cggcatcatt ttttgcgcca gcgcgtacac atacgcctcg gcatacagaa aatcgttgcc
     4681 aaacagatac ggtagaaagg agagacggtc gcactcttca acgttcagag tgacaggttt
     4741 cgcatattgc atgatgtttt cctccgttgt tcacatattc cgcgacaccg ggcggtgccg
     4801 tgcctccata aaagccgggc gataaggcgg ggatgcacag ggcgcaaggg aagccgcagc
     4861 ctgacgcaca cggctggcgg gagttggagg gtgagaccgc gaaatctgag cgggtgaacg
     4921 accggaaaat cacggcagac gggaaggagg gaccgggtga ccgcagcggc gaagcgtacc
     4981 cttgcatccc tgccagcccg gctaagatgg aggcacggca cagcccgcac acgacgctga
     5041 ggccacataa ctgaaaaacc acaaggccat gccctgcatg gccgggtaaa gcggagccgg
     5101 cggcgacttc ctttgtgctc tgtccgggga ccggcaggaa aaaggcgggg atttaccccg
     5161 ccaacgtcat catttccgga tttttgccag ggcatccgcc atgctgcgga tttcggcccg
     5221 ggcttcccgg accacctccc gcagaacacc acggtcaaac cagcggcggg gcggttcatc
     5281 cggtatacgc agcacatcgc aggccagccc actggacagg atatgctcaa tacaatcgct
     5341 gaccaggtac atttccaccg acgaaaaacg tgctccccgg ttcacgatgg catcgagcac
     5401 cacgggcagg ttttcgtcat cttcatcgag ccagcagggg tgatcgcttt ccttcacaac
     5461 cagacgaaag cggcgcgtct ggcgagtcag caacgtctga ccggtggtgg tcagcgccgc
     5521 ccggatacgc cgcgcatctt ctgccacccg gcaactgaca ctgcgtaccg catcgcgcac
     5581 cgccgcaagt acagccctga tttcgtcttc ggtcagccat tctccggcat acgggaaaga
     5641 cacctgcgtt ccgtctgccg gaagtaccgg cagcgactgc cactgactgc cggacagtac
     5701 cagttccgcc ccggttccgg tcagtttctg aatggcggag gccaggagcg gcggcagtac
     5761 gccgccgtga cggttgataa gtaacgtcgc cagccggggc gcatccagca gaacataaat
     5821 gctgatccag cccggatact tcgggttacg ctggtcaaca tccgtctctt ccggccagcc
     5881 ctgcgccagc agcaggttgc gggcgtgacg acaaatcagt gcgttcagcg tgctgttttc
     5941 gttcatgatt aagcgttctc cctgtatcgc cgcccgcagg gcaggcggcg ataacatcag
     6001 tcgttttcgt gtgtgttgtt tttcagcagg gtgcgggcct gattaaccac ctcctgacgg
     6061 cgggtgacgc attccgggac ggcgaggcca tgatcccggg cggcccattc acgtttccac
     6121 aatgcctgtt cagcggtctg aatcagggac gccagcagtt cagcagggac ggtcaccagg
     6181 cggttttcgc tctcgttgcg gataatctcc atcaccgcag cggaagaaat accggactca
     6241 atccgctggt cttccgggat gtcctccagg cgtgccagga cggtcctgat ttcatgaccg
     6301 gtcagagaaa tttccatacc gtcagccagt gcctgaatct cttccgggga ccagataacc
     6361 agcatcagcg gcatgtctcc cggatacttt actgccagct cccggcatag cgtttcgcat
     6421 gttccgtaca ttctcgtctc cttctcaatc tttaaagcac gcttccagtt ccagaatttc
     6481 ggtatatgcc tccatcagga ggcggtttgc gcgaaacagc gtctgacgca ccagacgttc
     6541 cggcagggtg tcggcattaa agctgtaccg ctgctccatc tcattcatgg ttgtacgcag
     6601 gtcgcacatg gcattggcca cctccgccac ttcggcgcgc agtttttcgt gtcctgcata
     6661 gttcatttac gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc
     6721 ccgttgcacg gcggcaaggc gttgctgccc ggcacggtga tactgctcaa gcagctcgat
     6781 accgatatac cggcgtccgg actggagggc ggcgacgcag gttgagccgc tgcctgcaaa
     6841 cgggtccagc acaattgcgt tcgggtgtgt gaagctctca atcagcggtt gcaggctggt
     6901 aacgggcttt tccgtcgggt gatggcgatt gcccgaatat ttccagccca gcacgtccgg
     6961 cagcgggttt tgtggcagac gtggacggcc ttttgccagg atgtaggcac attcgtggcg
     7021 atagcccaca tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac
     7081 gctgaatccc gcatttttcc aggcggccat aaagcgatcg acgcggttcc agccatagaa
     7141 gctcaccatt aacgcgtctt ttttgagtac gcggtacatt tcattacagg ctggttgcag
     7201 ccattcatcg gttttatcgc cggcgatggt gcgcccggaa cggtcacgga aaccgacgag
     7261 atacggcggg tcggtgagga tgaaatcgac agcattgccc ggaaacgtgg ccataacacg
     7321 cacgcagtca cccaggataa aacgggacat aacagcactc cttctcttac agagggcacc
     7381 gggagggcac ccctgtggtg cccgttcccc gctgcctgtg ggctgttcac cgcgtccggt
     7441 aacgggtcag gccgaccgtg gaataccgca gccgtcgtct ttttgacggc gaggatatgc
     7501 cgcgaaagcg gcttgaaggg cgttcatcag aaaagggcga cgggctggcg tacactgtca
     7561 ccaacgccag ccgggagcgg taaaagcgcc cgtcccgtgc ccggccggac gcgggacact
     7621 gaccacaggc agcgttcctg ccgcgccgcg tcagccggcg cagtaaaaag aggagcccgg
     7681 cgacatcttt tgttacccgc caaacaaaac ccaaaaacaa cccataccca acccaataaa
     7741 acgccaaaac aagacaaata atcattgatt gatggttgaa atggggtaaa cttgacaaac
     7801 aaacccactt aaaacccaaa acatacccaa acacacacca aaaaaaacac cataaggagt
     7861 tttataaatg ttggtattca ttgatgacgg ttcaacaaac atcaaactac agtggcagga
     7921 aagcgacgga acaattaaac agcacattag cccgaacagc ttcaaacgcg agtgggcagt
     7981 ctcttttggt gataaaaagg tctttaacta cacactgaac ggcgaacagt attcatttga
     8041 tccaatcagc ccggatgctg tagtcacaac caatatcgca tggcaataca gcgacgttaa
     8101 tgtcgttgca gtgcatcacg ccttactgac cagtggtctg ccggtaagcg aagtggatat
     8161 tgtttgcaca cttcctctga cagagtatta cgacagaaat aaccaaccca atacggaaaa
     8221 tattgagcgt aagaaagcaa acttccggaa aaaaattaca ttaaatggcg gggatacatt
     8281 cacaataaaa gatgtaaaag tcatgcctga atctataccg gcaggttatg aagttctaca
     8341 agaactggat gagttagatt ctttattaat tatagatctc gggggcacca cattagatat
     8401 ttctcaggta atggggaaat tatcggggat cagtaaaata tacggagact catctcttgg
     8461 tgtctctctg gttacatctg cagtaaaaga tgccctttct cttgcgagaa caaaaggaag
     8521 tagctatctt gctgacgata taatcattca cagaaaagat aataactatc tgaagcaacg
     8581 aattaatgat gagaacaaaa tatcaatagt caccgaagca atgaatgaag cacttcgtaa
     8641 acttgagcaa cgtgtattaa atacgctcaa tgaattttct ggttatactc atgttatggt
     8701 tataggcggt ggcgcagaat taatatgcga tgcagtaaaa aaacacacac agattcgtga
     8761 tgaacgtttt ttcaaaacca ataactctca atatgattta gttaacggta tgtatctcat
     8821 aggtaattaa tgatggacaa gcgcagaacc attgccttca aactaaatcc agatgtaaat
     8881 caaacagata aaattgtttg tgatacactg gacagtatcc cgcaagggga acgaagccgc
     8941 cttaaccggg ccgcactgac ggcaggtctg gccttataca gacaagatcc ccggacccct
     9001 ttccttttat gtgagctgct gacgaaagaa accacatttt cagatatcgt gaatatattg
     9061 agatcgctat ttccaaaaga gatggccgat tttaattctt caatagtcac tcaatcctct
     9121 tcacaacaag agcaaaaaag tgatgaagag accaaaaaaa atgcgatgaa gctaataaat
     9181 taattcaatt attattgagt tccctttatc cactatcagg ctggataaag ggaactcaat
     9241 caagttattt tcttaccagt cattacataa tcgttattat gaaataatcg tttgcactgt
     9301 ctctgttatt caggcaattt caataaaggc acttgctcac gctctgtcat tttctgaaac
     9361 tcttcatgct gcatttcgat ttttcttcta aaatcataac ttgcatacct gttcttttta
     9421 tgtatatcca gatctatttt tgtgacgagt tcaaaccaca atgatagcac tccccatgca
     9481 gcgttatgaa acatttccgc aacagattta gagtctgtat taaccgcttc agtcatgtag
     9541 tcgctaatac gttctgcagt gattttcgtt atttcatcgt aattcatttt atcccctaca
     9601 agactattta atggaatgaa aaatatttgg tctgataaca atgctcctga attaacacct
     9661 gcaggctatg ttaaagttgc cctcacacac aatctacctg ttttctcgtg tgtgtcacag
     9721 gcgcaataca taaatagtct ggccattaag ttcgtaacgg atttcgtact cgccagcaaa
     9781 aatccgtctg acatccctgg tttcaaactg aaacaactgt tcatcccata cgtggatgag
     9841 ttaacagaat gaccggcgcc tgtgtcaggg actacaccgt tttggctgca gctggtttac
     9901 tgaccaacgc cagaaaatca tataactgcg tcaatgtcag cgtatccagc gcgactgtat
     9961 tatttttctc tgcaaccaga atattgcatt aggttattac caggtgttct aatgtacagg
    10021 taaagacgtt ctttataatc tgaaatctct acttttaatc cctcagactt tgcctgctga
    10081 aagccctcgt caaggtgcgc ttttattagg ttaatgtctg gatgtcttgt atcgggggca
    10141 attctgtatt ggtgtaacga tggggtggat gttccctgca ataagacctc cagatagaga
    10201 ttggcacaac cattcccatt atttctaata gtttattggt aatcacaaac tgtatattta
    10261 gccattatat tgaatcctgt gaagtaaacg gcgacggctc aaaattttat caaacctaag
    10321 ataattctac catcaaagtt aataatttga atttatatga cggctctatg ggggggtaaa
    10381 aatccccgtg cagagaggcg attttcagag ttgaaaacgt cgtcgttaag aacaccaaag
    10441 tcagtaccac caacccggaa agtagcttca tgcaccggga caacagcagt tttggtgtcc
    10501 gaaatcgtac aattctgtac atctacgcaa ggaaatgtac ggaagtaagc caaatggtta
    10561 tcggacggtc ggaacatttc gtacagcctt tacgttcagt gagttagctg gcctagatgt
    10621 aaccactcgt tcaactcaat tctcttagcg tgggaaaatt tccgttttcc aagcggcccc
    10681 ctcaagcgga ccccttatag tacaataatt ttcgatatcc aaactgcccc caattacatc
    10741 tccagtctct ggcagcagag atcatatatt tccattgccg ccgtagccac gatattctgg
    10801 ctattcacaa aaacaattta gccccaaagc gcagtaaaaa agtacactat gaaggtgtat
    10861 gataccttca tagtgcttaa gtaattatga gtaatataat ctgtgtaaac tactaattgc
    10921 ttcaccgtca gtgattaaat gcgtagctgt tccttgtctt atataatacc tattttcaaa
    10981 atgacagact tctccgacac tttttatttc gatttttaaa acatgcagtc cttcatatga
    11041 acataatgaa atatctttta gtatttgttg tttcaaagcc tcattaaaat taaatgattc
    11101 tattttttgt tttatgaaca gaaagtaatt atctatgttt tttgattgaa ttacagcttc
    11161 atgatcaatt ccattgatat aatagccatt gcactcaata ggggtaacac catataaagt
    11221 cttaactctg tctgccgtag ctttggtatc ggaaacacca actaaaatat aaccattaga
    11281 ttctctaccg atgttattta tggcgacagc cgtttggact atattaatga aacattcttc
    11341 gtctatttta ttctcgcctg agagaataaa taatccctgt t
//
LOCUS       DADLZM010000129        11242 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.130, whole genome shotgun sequence.
ACCESSION   DADLZM010000129 DADLZM010000000
VERSION     DADLZM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.130"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..757)
                     /locus_tag="JNY73_003275"
     CDS             complement(89..757)
                     /locus_tag="JNY73_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY8049180.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1060..1653)
                     /gene="tehB"
                     /locus_tag="JNY73_003276"
     CDS             complement(1060..1653)
                     /gene="tehB"
                     /locus_tag="JNY73_003276"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY8049181.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1650..2642)
                     /gene="tehA"
                     /locus_tag="JNY73_003277"
     CDS             complement(1650..2642)
                     /gene="tehA"
                     /locus_tag="JNY73_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY8049182.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            2766..3746
                     /locus_tag="JNY73_003278"
     CDS             2766..3746
                     /locus_tag="JNY73_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY8049183.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(3741..4277)
                     /gene="rimL"
                     /locus_tag="JNY73_003279"
     CDS             complement(3741..4277)
                     /gene="rimL"
                     /locus_tag="JNY73_003279"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY8049184.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4340..4564)
                     /locus_tag="JNY73_003280"
     CDS             complement(4340..4564)
                     /locus_tag="JNY73_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY8049185.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(4704..6359)
                     /locus_tag="JNY73_003281"
     CDS             complement(4704..6359)
                     /locus_tag="JNY73_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY8049186.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(6583..7926)
                     /locus_tag="JNY73_003282"
                     /pseudo
     CDS             complement(6583..7926)
                     /locus_tag="JNY73_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            8143..9066
                     /locus_tag="JNY73_003283"
     CDS             8143..9066
                     /locus_tag="JNY73_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8049187.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9104..10744)
                     /gene="trg"
                     /locus_tag="JNY73_003284"
     CDS             complement(9104..10744)
                     /gene="trg"
                     /locus_tag="JNY73_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY8049188.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            11120..>11242
                     /locus_tag="JNY73_003285"
     CDS             11120..>11242
                     /locus_tag="JNY73_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8049189.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRT"
BASE COUNT         2892 a         2829 c         2621 g         2900 t
ORIGIN      
        1 aaaaccgcgc cttatatccc cgccctgaag gatggggttt tacggcgcac cggataaaaa
       61 gcgccatcta acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat
      121 cggtcgccat gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt
      181 tattcaggtc tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg
      241 catcaatcag ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca
      301 tttgcgtccc cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt
      361 tgctggtatc tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg
      421 taaccactgg tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa
      481 ttttatccag aactttctgc cctacctggg tagaaggttt tggtaccgga taataagtga
      541 ttgggttcca gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta
      601 aaacaggttt acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac
      661 cagaatattt atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc
      721 ataaagctgc aactttcgca aatgatgtgg tacgcatgaa tagtttcctg agatgattat
      781 attactttct aagttatagc aaaaagcgcc gcttcgtgtt gtggcaaaaa aagtgttggt
      841 gaaaataaat ttttcagccc cttttcagac gacatagtca atgccgaaaa aggggctaag
      901 tcaatcatca gtagttaaat ttcagtaagt taagctgcta aaaaacttcg actactgtga
      961 ttaatttcac atcccttcga taaaaaatat gcttaccgaa aagaagaaca gtaaatcatc
     1021 tcagcgtggg gaattttcac gcgttttgtg cttaccgggt cattttttac gtgccagcat
     1081 cgtggcgaaa cgcagtttaa tacgattacc gttggcgtcg gtgcggtgca gctcgccgac
     1141 gtcttcattg tatttcaccc tctcccagcc ttcgtaatat cgacgtaatt ctccctcttt
     1201 gaaggcaaac gtgaagccga cggtacatgg ataatcagcg gtatccatcg ccgccacaat
     1261 caagttgtaa ccaccaggtt tagtgcaacg ttgcatattg gcaatcaacc ctgggatggt
     1321 tttagcctca aggaacatca gcaccacagt cgaaagaata aaatcgtact gtccatcaaa
     1381 tgtgaggtta ttcagatcaa cgacccgggt gtgtaaatta tccagatttt caatggattt
     1441 aatgcgctcg acgttggcga tactcatggc atttttatcc catgcgtcaa catcataacc
     1501 attggctgcc aggtaaagac tgttacgacc attgccacag cccagatcca gcgttttacc
     1561 cggtttaacc actttcaccg cttccagtac ttcagagtgt gtgcgggtta attcatattt
     1621 atcagtaaaa tagttttcgt cacgaatgat cattctttgt cctctgcttt cattaaaacg
     1681 gcgcgctcgg ttctgactaa caattttccc tgcatcagaa gcgcaaaagt acggatgagc
     1741 agtattgcaa taataaaatt ggtaaagata aacagcggca ccgccagcgt atggaaaaat
     1801 ccattatcgc tgccactccc cagatgcaaa ccggtggttg ccagtgcaga tacgccgaac
     1861 gagaaactcc agaatgaagc attaaatggc tgggagagat accatggcat cagacgtagc
     1921 ataaacagca gttgcagcag tccataaccg aaaagcattt tcgccagcgt gtcaccctcg
     1981 ccgccgttga cgctcagcca ggcactacag gccaccagcg caggagcgag ctgaatgccg
     2041 agtgatgtcc gcagtgccgt gggtaattct cccgaactgc gcagacgctg caagatcacc
     2101 ggttcaaggc ttagccatga gaaaacgcct gcgcctaaaa acaccagacc ggcgtcggtg
     2161 tagcccaacg caccacaggc cattgcgctg ataaagttgt tggcaactgt cggcagatac
     2221 agtccaggcg tggtagcttc ctcagggtga gatccgcgcc ataatcccgc agtttgccag
     2281 gcggcataag ccaactgaac cacgacacca aaactgaaca tgcacaccgc cagtgggcga
     2341 aaccacggaa caaaaccaat cgccaccagc atcgtcgttg ccggaaacaa actcacaaaa
     2401 ctgctcagca ctggatggcg aacttccgcc agcacgctat gcggaaagcg tatgagtcgg
     2461 gtaataaatg cgctagtcaa taatccccag atgatcatcg ccagaatcac cagcccatcc
     2521 cctaaccagt ggctgacctg ccaaacctgg ctggcatagc gccaggcaaa tcccatccct
     2581 atcgtcccca acacaatacc aaagtagcct gccggcaaat tgagcacttt atcgctctgc
     2641 atcttcttac tcattttgtt taatttataa agtatatttg aaatgcattt tatggaattt
     2701 tctcgagcat agccagagcc gcagaatttg ctacggttat agtaagtcac ctgtgggaaa
     2761 acgctatgcg caaatatcgc ctaagtgaag aacagcgagc ctttagttat caggaagatg
     2821 gcactaaaaa aagtgtgtta ttacggcaga ttatcgccat tagcgatttt aacgatgtga
     2881 tagcaggaac cgctggcggc tggatcgatc gcgaaacggt gttagcacag gaaggaaatt
     2941 gctggattta cgaccagaat gctattgcgt tcggcggaac ggtgatttcc ggtaatacgc
     3001 gtatcaccgg caccagcgtg ctgtggggag aggtttacgc aacggataac gtctggatcg
     3061 ataatagtga aatcagccag ggtgcatata taagtgacag tgtcaccatt catgactcat
     3121 tagtttacgg gcaatgtaga atatttggtc acgccttaat tgatcaacat tctatgatcg
     3181 tcgccgcaca aggcttaacg cccgaccatc aactcctgct ccaaatttat gatcgcgcaa
     3241 gggttagcgc ctcgcgtatt gtccaccagg cacaaattta tggcgatgcc gtcgtccggt
     3301 atgcttttat tgaacatcgc gccgaagttt ttgattttgc cagtgttgaa ggtaatgaag
     3361 agaacaacgt ctggctatgc gattgcgcta aagtctacgg tcacgcgcag gtgaaagctg
     3421 gcatagaaga agatgccatt ccgacgattc attacagttc gcaggtggcg gaatatgcca
     3481 ttgtggaagg taattgtgtg ttaaaacatc atgtcttgat tggcggaaat gccgtggtac
     3541 gtggtggacc gattctgctc gacgagcacg ttgtaataca aggtgagagc cgtattacag
     3601 gtgcggtgat aattgaaaac catgtggagt taaccgacca cgccgtcgtt gaagcctttg
     3661 atggcgacac ggttcatgtc cgtggtccaa aagtcattaa cggcgaggag cgcattacgc
     3721 gaacgccgct ggcagggtta ttatgaatcg ataatacgcg cgtataagtt cacatcatca
     3781 taggcatcat tcaggaactc agcctgtttc aggcaacctt caaggataaa accattgcgc
     3841 aaagcgacct ggttgctttg cggattgtcc acccgacatt tgatcacgaa gcgtctaagt
     3901 tcaccagact gagcgtaatg atgaatcaat gcctgcagcg cctgagaaat gatcccctgc
     3961 ccctgatgag attcgtccag ccagtagccg atttcagcag ttttattcag tggttcaata
     4021 cgattaaacg agataacgcc gataagttca tcttctttga aaatcatgaa cattttggca
     4081 tagccgcgtt gatgcaacat cacattaccc tgcaccgttt ttcgcgtgtc ctcttcactc
     4141 tgaacgaact gcggccagtt tagcgactgc tgtaaccagg ttttattttt acagattaac
     4201 tgataaagag gtgtgacgtg actttccgca acagcatgta attcaagtga ttcgcttact
     4261 tttatcgttt cagtcatctt atctccagtc aatccatgct gttgttaaaa aagccgcctg
     4321 gctggcggct taggaaagtt tacacctctt tgacgctctc ctgctgcagg attttgagca
     4381 tctcatcttt taactgtagc ttttgttttt tcatgcggac cacttccgca ttgtaccctc
     4441 gaccgtcgga accttccttt ctggcaattt catgatcaag tttattgtgt ttatcgaaca
     4501 aggacataaa gcgaggattt tcgtttttca gacgggatat taaatctcgg tattctggaa
     4561 acatacgcac tccctgttag tggctgacac ggtgtatctg aaatacacac catcagatta
     4621 ctcatttaac ttagggaaaa atgcgagcgg gatcgtgttt aaccagacgg cacctcccga
     4681 agaaggtgcc gaagaaaaaa cgattaactc atcacgcggt cgtcaacata ctgacgttta
     4741 tccggcgctg gcgggaaata ctgatacagc catgtttcac tgatagcgtc cccctgacaa
     4801 cgtagataca tccgcatatc gaccggatca gtggagtccg aagtcggata ccagtcaaac
     4861 tgaatacgat aaccatcgat gggttcaata tagagaattt cgatttgctt cgcttcccca
     4921 ctggaaagcg taatcaccgg ctcaatgcct tttggcgcgg cagctttcag atcaccacca
     4981 acgaaatcga cggcaaaacg acgcgcccat ttttcgggat agtgttcacc tggcgcccaa
     5041 ccttccggga aaccgcccat gccggtacgc gtcgccataa cgcgcgctaa tgggcaatga
     5101 acaggcggtt gcgcactcca gtacagacga tactggaatg caaactcatc acctgctttt
     5161 acagcttttt ctggctgcca gaagcagaca atgttatcca gcgtttcgcc cgttgttggg
     5221 atttccatca ggccgatggt gcccttaccc cacttgttac gcggttccac ccacaggctt
     5281 ggacgtttgt tataccagcc cataatgtcc tgataatggg agaagtcacg atccagttgc
     5341 aataaaccaa accctttcgg gttgttgtcg gtgtaagcat tgaactgcaa tttttgcgga
     5401 ttattcagcg gacggcaaat ccactcgccg ttgccccgcc acatggacag acgatcagag
     5461 tcatgaattt gcggatgaat tgtatcgcac atccgacgtt cattagtacc gcagctgaac
     5521 atactggtca tcggcgaaat gcccagctgt ttaatgtctt tgcgcgcata caggtgattt
     5581 tccacatcca taatcacctg acttttctca caatggatag tgaacttata ggcaccagta
     5641 atgctggcgc tatcgagcaa cgcataaacg gtaaaggtag ttgcccccgg ttttaccgta
     5701 tcaaaccaga aggcggtaaa gtcggggaac tcttctttac tgtcggtgta agtgtcgatc
     5761 gccaggccgc gggccgacaa accgtattga tatgtatcat caacggcgcg gaaataactc
     5821 gcgccgagaa atgatactac atcacggcgc gccagttcgg gggctttaaa cacgcgaaaa
     5881 ccggcaaagc cgagatcgct ttgcccttct aattgtttgg tatcaacacc tgcatcgttg
     5941 tatttgaaca actccgggcg aaagtgaatt tcacgcgcca gatgtgttgc tggatctaca
     6001 gaaaacatac gaacgcggcg acggaatccc attcccatat ggaagaactg agcgtccagt
     6061 tgacggttct caacgttatg ccagagcgat ttttcggcgt cgtattgaat actgttataa
     6121 gcctgcggcg tcattgtcgc cagcgtgtca ggtaacggac gaggcgcacc acgccacgct
     6181 gtttgcgcta agtcgtgcgc cattgactgt agaatggaga agtcaaaacg ctgggtttgc
     6241 ccgtcggcaa tatcagaatc tgccgcgaat gctgcctgag aaaaaagaga agcaatgccg
     6301 ctggtaccgc acacggcggc catagccatt gaacctttaa taaatcgtct acgatccata
     6361 cctgaaagtg agtccttctg gtgttgtgaa tggtcccgcg ctggtcgttt tcagcggcaa
     6421 gaaagtgctt atttatgacc gctcactcta gacaatattt attaataatc caattgttga
     6481 actgagagaa tatgcataaa tcggcatttt tttatgtcga tgggatgagc ctgattcgtt
     6541 tgtaaaatca ggcaaatttt tgcaaaggcg gatggtcaga atttaaagca aaccacagcg
     6601 acgtttactg cgttcttccg cttcttggta aagctggaac tcatccaaaa ccggacaacc
     6661 caacgcctgc tcaaatgctt cgcgactggc attaccgtga ctgcgtgtct gcgtttcatt
     6721 acccagattt gactggaaga tccccgccgc gctaacgggc aagaaatctt cataggtgat
     6781 gggttgcgcc actacccaac cacgttcaat taagggctgt ggatcgtctc caggatgaat
     6841 cgcctgacga tgcgcctcac ccgaaggcgt cagacggtac cggaaccatg ccaacccttg
     6901 ctgacgcatt aaaaactcac tgtcaggaaa agtgcggaag gtttcctgta aatgcatttg
     6961 gtgagtgaga ttatcctgcc cggttccagc gttacgcaga agatcatcat acagttgtcg
     7021 ccctttcggc gttaatgcca cgccacgctg ctcaatttca ccaaagcgcg cggtatgcgt
     7081 gccctgtttc tgccccgcaa acaacaccgt ctcttccagt gctttaaagc tggtctggcg
     7141 tagtaaaatc ggtacctcgc ggcgcggcgg cccctcgatg agaattttgg gttcaattcc
     7201 gcattcaggc atcatcgact gcacccggtc aatatccagc gtacgtggcg tcaggtggtt
     7261 gatatggcat ccaggaaaac agaccacatc agcaatcaac cgatgttcgt tgtgcaaggc
     7321 gcgataggtt tcttcatcta ccgttgctaa ctagtgccag cgaaacgttt ccagggcttc
     7381 ctgcacaaac tcctgtgcct gtgtttcgtt aaaaccgccc tgctgctcat attcctctaa
     7441 cagttgtcga caacgtgggg tgaagatatc gcgctgacgc agaatctccg ccgctttctg
     7501 gcgcaaaatt tcgttctcga taagctcaag gcggagtaag gatgtaaaaa cgcggaaggg
     7561 attacgcgcc agagaagcat catcaatggg ccgaaatgct gtcgaatgta ccggcacccc
     7621 tgcctgcgag agatcgtaat agctcaccgg gtacatcccc ataatggcaa acatccgccg
     7681 aagagtagca agctcttgtg cagtcccaac gcgaatcgcc ccatgacgtt caacattcag
     7741 tcgcgccagc tcgtctgcat ttaccatttt ttcgtgcagt tgaggattgt tttccagcac
     7801 agccagattc acatcagcta ccagttccag cagcgtgcca tattgcggaa cttcttgctg
     7861 gtacatggct gacattgcct gcgaaaactg ttcccgaatc tcatccgccg tgatgctgtt
     7921 cgccatagtg tctgcctcca gtggtcagta atctggagtg taggtaacag cattcactct
     7981 ggcgggaaga atttacaaac tgtgatctcg ccgcgaaaac atcaatatta tccattttgc
     8041 tgtaacataa ttgctttaat tgttaataat attttacaat caagttatca taatcaaaca
     8101 acttcacttg tcagcgacac cgcttcgttt ttaacatcgc ttatggaaaa aaatagtctg
     8161 tttagtcagc gcatccgttt gcgccacctt catacattcg tagctgtcgc acaacaagga
     8221 actttggggc gcgcagctga aacccttaat ttgagtcaac ctgcgctctc taatacattg
     8281 aatgaactgg agcagctgac gggcgctcgc ttgtttgagc gtggtcgtca gggggcgcaa
     8341 cttaccttac ccggcgaaca atttttaacg catgcagtca gagttcttga cgccatcaac
     8401 actgccggac agtcgcttca tcgtaaagaa agtcttaata atgatgtcgt cagggttggt
     8461 gcactaccta ctgcggcact ggggatatta ccttcggtta taggtcagtt tcatcagcaa
     8521 caaaaagaga cgaccttgca agttgcgaca atgagtaacc ctatgattct ggcgggttta
     8581 aaaaccgggg aaatcgatat cggcattggt cggatgtcag atcctgaact gatgaccggg
     8641 cttaattacg aactgctgtt tcttgaatcg ttgaagctgg ttgtccgccc taatcacccg
     8701 ctacttcagg agaacgtaac gctaagccgg gtgctggaat ggccggtcgt tgtatcacca
     8761 gaaggcactg cgccacgcca gcattcagat gcattagtgc agagccaggg ctgtaaaatt
     8821 ccttcgggtt gtatcgaaac gctgtctgct tcgctatctc gtcaacttac cgttgaatac
     8881 gattacgtgt ggtttgtccc ttctggcgct gtaaaagacg acctgcgtca tgccacgctg
     8941 gtggccctgc ctgttccggg acatggtgca ggcgaaccga ttggaatact gacccgcgta
     9001 gatgcgacgt tctcttctgg ttgccagttg atgattaacg ctattcgaaa atcaatgccg
     9061 ttctgaaagg tgaagggatc tgtcgatccc tccttgaaca ttttcacacc gtagcgaaac
     9121 taactggttc acccgctccg cgaggttctg ccgacacaga atgtttgttc agacggaata
     9181 cgttcaccgc ctcagttaat cgtgcagcct gttcttcaag ggacaccgcc gctgctgagg
     9241 cctcttctac cagagaagca ttctgttgcg tcaccttatc catttcagaa atcgcctggc
     9301 taacctgagt tatgcctcta ctttgttcat ccgaggcggc ggcgatttcc tgcatgatat
     9361 gtgtgacact cgcgacggca tcaacaatag tgctcatggt tttccctgcc gttgccacct
     9421 catccgaccc caggtcaatt aacctgactg attcactgat caagccttca atctctttcg
     9481 ctgcctgagc gctgcggctt gcgagtgtcc gtacttcgct ggcgacaacg gcaaatccac
     9541 gcccttgctc acccgctcgc gcggcttcaa cggcagcatt cagtgccaga atattcgtct
     9601 ggaaagcaat actgttgatg acggcggtga tctcagaaat tttcttcgaa ctcgtggaga
     9661 tagcgcccat cgtttttact acaccggaaa ccgtctgccc gccatcgctg gctttaatag
     9721 aagcctcttg cgccagtttg ctggcatgat gcgcgttatc cgcattctgt ttcaccgtcg
     9781 cagtgagttg ctccatgctg gcggcagttt gttcgatagc cgccgcttgt tcttcggtgc
     9841 gagatgacag gtccgcattg ccagctgaaa tttcgctggt gccacgataa atctcttccg
     9901 cgccctgtcg aacagtccct actgtcatcc ccagtgaatg ctgtatttgc tgtaaatgac
     9961 gacttaagcg accgatttca ttacggcccg ccggttcatc attcatcgtc agatcgccac
    10021 tggcgatttt ttcaatccgt tgtgcggcat gttgcagtgg acgaatgacg atccgacgta
    10081 gcaccataaa tgttatcagc gtcatgacca gggcaagcac aaacgcgcca atcatgaaca
    10141 tcccacccag gcgggtgcgc tgatgggcca gttccgccag ttggttggct ctggtgctac
    10201 gtatcttaac ggctttgttc aaaatatcgg tataagcatt atccagcggt cggatctgct
    10261 cactttcatg attgataatc gcttcaaaca tgccattttt ggcatatttc aacataggtt
    10321 gcatacccgt gatataagcc tgaaagcgtt gatttaattc agtgtcgagg gcttcatcag
    10381 caggtgtttt caccggtcga ttctgataag cacgataacc ttgctgcgac tgtttaatct
    10441 ccgattcggc ttgcgcaata tttcgcttca ttgcttccat ttccgcaata cgactcgcag
    10501 ccccggcctg aatcatattg atccgggctg aacgcaaaaa atctgaactg ttcgctaacc
    10561 cggtccgaat ctcaatctcc gcagtaacat cacgctgatc gcgatcggcc tgccagagga
    10621 aatagccagc cagagcagaa cttaatgcga atagaactaa gataccgcca agaatgcagg
    10681 caaataacgg aaccagcctg atgtgatgca aaaaacctaa tcgctgtgag ggagttgtat
    10741 tcattaccgt tgtctctcgt ccaggtttac tcctgataga aagtcatcgg cacttgacgg
    10801 taattactta tgaccaaaaa cctctttgcg tcacattttt acaacaatta ttgcagagaa
    10861 aattaatgct attggaatga agagagtaac gaatggctaa actggctaac ccgaatgacg
    10921 aaaaattctg gaaaacagca cgcaatttcg tgaagtgatt attttcgtcc ctaaggaaac
    10981 ctcataggat gcctgcgggt gcctgagact ttctgttttc aggctaaccg ggaatcagaa
    11041 agacgaaggc cccgaccgat atttctatca actgtactgc acccattttg ttggacgatg
    11101 aaatggaata gcccctaata tgtcaaagcc aaaataccct tttgaaaagc gccttgaagt
    11161 cgtgaatcac tacttcacta ctgatgatgg ttacaggatc atctcggcac gttttggtgt
    11221 cccccgaacc caggtcagga ca
//
LOCUS       DADLZM010000130        11183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLZM010000130 DADLZM010000000
VERSION     DADLZM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.131"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNY73_003286"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNY73_003286"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY8049190.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /locus_tag="JNY73_003287"
     CDS             complement(721..1749)
                     /locus_tag="JNY73_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY8049191.1"
                     /translation="MAAAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /locus_tag="JNY73_003288"
     CDS             1924..3516
                     /locus_tag="JNY73_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY8049192.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="JNY73_003289"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="JNY73_003289"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY8049193.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4801..5802
                     /gene="add"
                     /locus_tag="JNY73_003290"
     CDS             4801..5802
                     /gene="add"
                     /locus_tag="JNY73_003290"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY8049194.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5838..6878)
                     /locus_tag="JNY73_003291"
     CDS             complement(5838..6878)
                     /locus_tag="JNY73_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8049195.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7121..7246
                     /gene="blr"
                     /locus_tag="JNY73_003292"
     CDS             7121..7246
                     /gene="blr"
                     /locus_tag="JNY73_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY8049196.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7519..7734
                     /gene="ydgT"
                     /locus_tag="JNY73_003293"
     CDS             7519..7734
                     /gene="ydgT"
                     /locus_tag="JNY73_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY8049197.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7820..8260
                     /locus_tag="JNY73_003294"
     CDS             7820..8260
                     /locus_tag="JNY73_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY8049198.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8337..8918
                     /gene="rsxA"
                     /locus_tag="JNY73_003295"
     CDS             8337..8918
                     /gene="rsxA"
                     /locus_tag="JNY73_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY8049199.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8918..9496
                     /gene="rsxB"
                     /locus_tag="JNY73_003296"
     CDS             8918..9496
                     /gene="rsxB"
                     /locus_tag="JNY73_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY8049200.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9489..>11183
                     /gene="rsxC"
                     /locus_tag="JNY73_003297"
     CDS             9489..>11183
                     /gene="rsxC"
                     /locus_tag="JNY73_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY8049201.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2594 a         2885 c         2817 g         2887 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagtttact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggcagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttacc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa tcgttgcgca atcgtggatt
     4741 tttaccctgc tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc
     4801 atgattgata ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt
     4861 ccccagacca ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctt
     4921 gaaacactga ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg
     4981 accaaacttg actggggcgt taaagttctc gcctctcttg atgcctgccg ccgcgtggca
     5041 tttgaaaaca ttgaagatgc agcccgtaac ggcctgcgct atgtcgagct gcgtttttca
     5101 ccaggctaca tggcaatggc acataagctg cctgtagcgg gtgttgtcga agcggtgatc
     5161 gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg
     5221 agccgaacct tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt
     5281 gaccagatta ccgcacttga tttagctggt gatgaacttg gattcccggg aagtctgttc
     5341 ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa
     5401 gctgccgggc cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga
     5461 catggcgtaa aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt
     5521 ggtattgaat cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca
     5581 catccgctga aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc
     5641 ggcgtacagg gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta
     5701 tcccgtgagc aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgca
     5761 gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt aacaaaaatg gatggtgcaa
     5821 attgcaccat ccatttttca cgcaaggcac aacgtcgcgc gatgtttggc ggattcgatg
     5881 cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca
     5941 tcgccattta acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc
     6001 aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata
     6061 tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga
     6121 tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca
     6181 gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg
     6241 tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg
     6301 accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa atcgtaccag
     6361 ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg
     6421 tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt
     6481 agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac acgccccagg
     6541 ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca
     6601 acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg ggtatcgttg
     6661 ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag
     6721 acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc
     6781 gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa
     6841 ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta
     6901 tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc tgccagcaat
     6961 aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa
     7021 tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc
     7081 cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc ttattgaatt
     7141 aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga
     7201 caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc tgcacttgtg
     7261 gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa accaggaatt
     7321 tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc
     7381 agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac
     7441 aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct ttggtattaa
     7501 ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt
     7561 tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag
     7621 gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg
     7681 cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca ataaagaaag
     7741 tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg cctgtttgcg
     7801 tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct
     7861 tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta
     7921 taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac
     7981 acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg
     8041 gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct
     8101 gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg aagttggcat
     8161 agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa
     8221 acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt
     8281 tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga
     8341 ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt
     8401 ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg
     8461 ggctggcaac aacgtttgtg atgacgctga cgtctatttg cgcctggctt atcgatacgt
     8521 ggattttgat cccacttaat ctgatttacc tacgcaccct ggcatttatt ctggtgattg
     8581 ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc
     8641 tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc gtggcgttgc
     8701 tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt tccgccgctg
     8761 tcggtttctc gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt gctgtggctg
     8821 atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc
     8881 tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt
     8941 tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg cctcccgccg
     9001 ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca
     9061 gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg
     9121 tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt
     9181 gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat
     9241 ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc aggcttgtcc
     9301 ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg atctctgtac
     9361 gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc
     9421 agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt
     9481 ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg
     9541 gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg cgccaggtac
     9601 cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa ggtgagttgt
     9661 gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa
     9721 tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg
     9781 ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt gaagactgct
     9841 ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag ttaatcgagc
     9901 gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca ggcgttaaat
     9961 tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt
    10021 acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtattc
    10081 gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa gataacaaac
    10141 cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt tctctgcggg
    10201 tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga
    10261 agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca
    10321 ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgttgtaa
    10381 ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggaacgccag
    10441 tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg
    10501 gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta aagattacca
    10561 actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagctgca
    10621 tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa cagttgtact
    10681 ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc gactgcattg
    10741 aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa tatttccgtc
    10801 aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag
    10861 cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agctgctcgc cttgaacgac
    10921 ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg
    10981 cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac
    11041 gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga
    11101 aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca cgtaaaactg
    11161 ccgttgaagc agctatcgcc cgc
//
LOCUS       DADLZM010000131        11093 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLZM010000131 DADLZM010000000
VERSION     DADLZM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.132"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            215..2920
                     /gene="malT"
                     /locus_tag="JNY73_003298"
     CDS             215..2920
                     /gene="malT"
                     /locus_tag="JNY73_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY8049202.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWNLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALVQQTEQIARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2963..3769)
                     /gene="rtcA"
                     /locus_tag="JNY73_003299"
     CDS             complement(2963..3769)
                     /gene="rtcA"
                     /locus_tag="JNY73_003299"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY8049203.1"
                     /translation="MGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADG
                     PSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFYPAGGGVVATEVS
                     PVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSLHEQNIHNLPRDQ
                     GPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASPAAVGEYLADQL
                     VLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTRVSIE"
     gene            complement(3844..5070)
                     /locus_tag="JNY73_003300"
     CDS             complement(3844..5070)
                     /locus_tag="JNY73_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY8049204.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5259..6857
                     /locus_tag="JNY73_003301"
     CDS             5259..6857
                     /locus_tag="JNY73_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY8049205.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKAAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6839..7597)
                     /locus_tag="JNY73_003302"
     CDS             complement(6839..7597)
                     /locus_tag="JNY73_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY8049206.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="JNY73_003303"
     CDS             complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="JNY73_003303"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY8049207.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="JNY73_003304"
     CDS             complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="JNY73_003304"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY8049208.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9005..10510
                     /gene="glpD"
                     /locus_tag="JNY73_003305"
     CDS             9005..10510
                     /gene="glpD"
                     /locus_tag="JNY73_003305"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY8049209.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10779..11009)
                     /locus_tag="JNY73_003306"
                     /pseudo
     CDS             complement(10779..11009)
                     /locus_tag="JNY73_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         2679 a         3037 c         2841 g         2536 t
ORIGIN      
        1 gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg tgagttgtta
       61 ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac gtcatcgctt
      121 gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca aaaaaaataa
      181 tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa aactaagtcg
      241 tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc
      301 gaacaacttc cggctggcgc tgatcacgag tcctgcgggc tacggaaaga ccaccctcat
      361 ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggatg aaggtgataa
      421 ccagcaagag cgtttcgcca gctatctcat tgccgccgtg cagcaggcaa ccaacggtca
      481 ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat gccagcctga cgtcactctt
      541 cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg tcatcgatga
      601 ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta ttcgccatca
      661 accagaaaat ctcaccctgg tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa
      721 tctgcgtgtt cgtgaccaac tgctggaaat tggtagtcag caactggcat ttacccatca
      781 ggaagcgaag cagttttttg attgccgtct gtcatcgccg attgaagccg cagaaagcag
      841 tcggatttgc gatgacgttt ccggttgggc gacggcacta cagctaatcg ccctctccgc
      901 ccggcagaat acccactcag cccataagtc ggcacgccgc ctggcgggaa tcaatgccag
      961 ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg caacgcgcca
     1021 ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gccctcatca cccgtgtgac
     1081 cggcgaagaa aacgggcaaa tgcgtctcga agagattgag cgtcaggggc tgtttttaca
     1141 gcggatggat gataccggcg agtggttctg ctatcacccg ctgtttggca acttcctgcg
     1201 ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg ccgccgcaga
     1261 aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg cggcaggcga
     1321 tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg aatttgttca accatagcga
     1381 actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc
     1441 gcagttagtg ttattgcagg cgtggctaat gcaaagccaa catcgctatg gcgaagttaa
     1501 caccctgcta gcccgtgctg aacatgaaat caaggacatc agagaaggca ccatgcacgc
     1561 agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg atgaagcgga
     1621 acggctggca aaactggcac tggaagagct gccgccgggc tggttctata gccgcattgt
     1681 ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc gaattgaccc gctcactggc
     1741 gctagtgcag caaaccgaac agatagcacg ccagcacgac gtctggcact acgccttatg
     1801 gagtttaatt cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga
     1861 aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat ctggaacagc tgccaatgca
     1921 tgagtttctg gtgcgcatcc gagcacaact gttatgggcc tgggcacggc tggatgaagc
     1981 cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct tatcagccac agcaacagct
     2041 tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag ataacgcccg
     2101 tagccagttg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca gcgactggat
     2161 ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg accggcgata aagccgccgc
     2221 tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact tcctgcaagg
     2281 tcaatggcgc aacattgccc gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat
     2341 tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg
     2401 taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg acgcccagcg
     2461 cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga tttatcagtc attttgtcat
     2521 cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt caacttaata cgctgccgga
     2581 actggaacag catcgcgcgc agcgtattct gcgagaaatc aatcaacatc atcggcataa
     2641 attcgcccat ttcgatgaga atttcgttga acgtctgcta aatcatccgg aagtgccaga
     2701 actaatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc tgatctactc
     2761 cggttacagc aatgaacaaa ttgcgggcga actggaagtg gcggcaacca ccatcaagac
     2821 gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca
     2881 cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga gtttagccgg ataatgcatt
     2941 ggatccggtt tacatcttta catcattcaa tactcacccg cgttacgcca tctgtttcta
     3001 tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc gatattggtc agcagatggc
     3061 atgaaggatg ggcgaccgta aattctcccg cgcccgccag cgccatcggc agtaccagtt
     3121 ggtcggcgag atattccccc accgccgctg ggcttgccag gtagcgtttc acctctttca
     3181 ctaactgcgc ggctaccacc tcggcactga cgcgcttttc accgacgacg aaaaagcgtt
     3241 cagtgatatt ttcactttcg acttccagcg agacggtatt acccggcccc tggtcgcgcg
     3301 gcaggttatg aatattctgt tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac
     3361 gctcagcaac atggcgcggc acaccagcta atagaacttc tccacgcatc tgcacaatgt
     3421 tcccgcgctc gccaagttgc aaggtgctaa acgatgccac gggcgagact tccgttgcca
     3481 ccacaccgcc tccggcagga taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc
     3541 ctattttcgc cagcagcggc tccagcaccc ggcggataaa atccgcaggc ggcgccgacg
     3601 ggttatcggt gccgccgctc acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg
     3661 gcagcaccgt ttgcagcacc agcgtacaac ttccggcgct accgatagca aagcggtaat
     3721 cgccgccgcg cacggtgcca ggccggaaga ccagacgctg cgatcccagc tcagcgccga
     3781 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
     3841 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
     3901 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
     3961 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
     4021 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
     4081 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
     4141 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
     4201 aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
     4261 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
     4321 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
     4381 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
     4441 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
     4501 tccacgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
     4561 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
     4621 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
     4681 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
     4741 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
     4801 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
     4861 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
     4921 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
     4981 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
     5041 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
     5101 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaatttt ttaatttatt
     5161 gtttctaaaa agattatatc tttatgtccg taaacggaga tttcccgcaa agccgattta
     5221 ccgataatga aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg
     5281 gctttgtcgg taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc
     5341 gtccgtcact ctgtatatgc cagcaagaat cgttggtcat cgatcgactg gaattattgc
     5401 acgatgcccg ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc
     5461 cagaaacaga agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg
     5521 tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact
     5581 atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg
     5641 aagcgcgtta tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc
     5701 cgcgcggccc aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg
     5761 ccagccgctt tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca
     5821 cgcgtaaccc ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc
     5881 gcgcgccgat tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca
     5941 tcttcgagtt aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg
     6001 ccaccctgcg cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa gccgcgttta
     6061 ccggggcgcg ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc
     6121 ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag
     6181 agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg
     6241 ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt
     6301 acgcgcggat caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata
     6361 ttgaaccgaa cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc
     6421 gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggctacat
     6481 ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta
     6541 gcggacgcat cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc
     6601 aggagagccg cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc
     6661 tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt
     6721 ctgctgccgg acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg
     6781 cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaggcc gtgcaggatc
     6841 agcacagctc cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg
     6901 gggcgtcggt gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac
     6961 ggccaaattt tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc
     7021 gcttggtacg aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc
     7081 tgatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc
     7141 caatgatccc gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt
     7201 cttttaccat caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt
     7261 gattgagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg
     7321 agccattggg gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg
     7381 tggctttacg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat
     7441 gatggcgcag gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg
     7501 ggctgacgga gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca
     7561 gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt
     7621 cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg
     7681 atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag
     7741 atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg
     7801 ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac
     7861 ggcccgctga atttttgctg cacatagccg cttaacaggg cgctgataag cgtaatgaca
     7921 attagcttac cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc
     7981 aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag
     8041 aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg
     8101 ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc
     8161 ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg
     8221 ccggtatggc ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga
     8281 aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg
     8341 ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac
     8401 gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat
     8461 tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact
     8521 gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca
     8581 gcagatactg cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag
     8641 tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct
     8701 gcaccgcatg ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt
     8761 tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc
     8821 tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat
     8881 aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg
     8941 attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg
     9001 cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc
     9061 ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg
     9121 cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta
     9181 tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc
     9241 gcatatcgcc ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg
     9301 gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg
     9361 atcaattggt ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt
     9421 cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt
     9481 ggtgcgtaaa ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa
     9541 cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc
     9601 gcgcggcctg gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca
     9661 tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt
     9721 gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat
     9781 cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc
     9841 gaaagcagtg aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca
     9901 ctttaaaaag cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct
     9961 gtgtgatgat gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca
    10021 tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg
    10081 aaaactggca gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc
    10141 gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc
    10201 cgctcgcctg cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacgcgcg
    10261 cacttacggc agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg
    10321 ggaagatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg
    10381 ggtccgccgc accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc
    10441 ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct
    10501 ggcgtcgtaa attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga
    10561 caaacacaaa actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat
    10621 tgaatttgca tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa
    10681 gcgcactttg tcaaaaatct aacctacttt ttaattcagg gaattaccgc aaagcccacg
    10741 ccatcatacc caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc
    10801 attaatgcgc ccagcgtcag cataaaaaca ttgagcagaa ggtaaattgt tggcgtaaag
    10861 agtaaattgc aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct
    10921 gcaaaaaatt gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc
    10981 cattttctat tttgcattat tctttccatt ctttctgaat ggcgaaatta tactcccctt
    11041 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt tgg
//
LOCUS       DADLZM010000132        10815 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLZM010000132 DADLZM010000000
VERSION     DADLZM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.133"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            93..1178
                     /locus_tag="JNY73_003307"
     CDS             93..1178
                     /locus_tag="JNY73_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8049210.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1249..1857
                     /locus_tag="JNY73_003308"
     CDS             1249..1857
                     /locus_tag="JNY73_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY8049211.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1955..3346
                     /gene="selA"
                     /locus_tag="JNY73_003309"
     CDS             1955..3346
                     /gene="selA"
                     /locus_tag="JNY73_003309"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY8049212.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3343..5187
                     /gene="selB"
                     /locus_tag="JNY73_003310"
     CDS             3343..5187
                     /gene="selB"
                     /locus_tag="JNY73_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY8049213.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5377..6528
                     /gene="yiaY"
                     /locus_tag="JNY73_003311"
     CDS             5377..6528
                     /gene="yiaY"
                     /locus_tag="JNY73_003311"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY8049214.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6693..8231
                     /locus_tag="JNY73_003312"
     CDS             6693..8231
                     /locus_tag="JNY73_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY8049215.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8450..8593)
                     /locus_tag="JNY73_003313"
     CDS             complement(8450..8593)
                     /locus_tag="JNY73_003313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049216.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            8776..9099
                     /locus_tag="JNY73_003314"
     CDS             8776..9099
                     /locus_tag="JNY73_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY8049217.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9105..10241
                     /locus_tag="JNY73_003315"
     CDS             9105..10241
                     /locus_tag="JNY73_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8049218.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQVR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10238..>10815)
                     /locus_tag="JNY73_003316"
     CDS             complement(10238..>10815)
                     /locus_tag="JNY73_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8049219.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2659 c         2977 g         2602 t
ORIGIN      
        1 atcgccgcac tttctgaccg aatttcccgg ctggagcatc aactcgccgc cgagaaaaag
       61 actgactatt ccacgttccc ggagatctaa ttatggatct attgattgtt ttaacttacg
      121 tggcgctggc gacggcggcg ctgggaggcg tgtttctggt gagtggtttg attttgttga
      181 tgaactacaa ccacccttac acttttaccg cgcaaaaggc agtgatagcg atccctatca
      241 cgccacaggt gacgggaatt gttactgaag tcactgacaa gaataatcag cttattcaaa
      301 agggcgaggt gctttttaag ctcgacccgg ttcgttacca ggcgcgagtt gacagacttc
      361 aggctgacct gatgacggcg acgcataata taaagacgct gcgtgcgcag ctcactgaag
      421 cgcaggccaa caccacccag gtttcagcgg agcgcgaccg tctgtttaaa aattatcaac
      481 gttacctgaa aggcagccag gcggcggtga atccgttctc ggaacgtgac atcgacgatg
      541 cgcggcaaaa tttcctcgcg caggatgcgt tagtgaaagg ctcggtggcg gagcaggcgc
      601 agatccagag tcagctcgac agtatggtca acggcgagca atcgcagatt gtgagcttaa
      661 gagcgcaact tactgaagca aaatataatc ttgagcagac tgtcattcgc gcaccaagca
      721 atggctacgt cactcaggta ctgatccgcc caggtacata cgcagctgcc ttgccgctgc
      781 gtccggtgat ggtcttcatc cccgagcaaa aacggcaaat tgtcgcccaa tttcggcaaa
      841 actcgctgtt acgtctgaaa cctggcgatg atgcggaagt ggtgtttaac gcgctacctg
      901 ggcaggtgtt ccacggcaaa ttgactagta ttttacctgt cgtgccaggc ggttcttatc
      961 aggcgcaggg ggtattgcaa tcattaacgg tcgttcccgg cacggacggt gtgctgggaa
     1021 ccattgaact ggaccctaac gatgatatcg atgccttacc cgacggcatc tacgcccagg
     1081 tggcggttta ctccgaccat ttcagccatg tttcggtgat gcggaaagtg ctgctaagaa
     1141 tgaccagttg gatgcattat ctttatttgg atcattgaga cttatatcac ctgtgttttg
     1201 cgtgagcctg agccatactg atatttttcc tcgcacaaag gactcggcat gaaactcgtc
     1261 ggtagctaca ccagcccgtt tgtacgcaaa ctttctattc tgttgttaga aaagggcata
     1321 actttcgaat ttattaatga actgccctat aacgcggata acggcgtggc gcaatttaac
     1381 ccgttaggga aagtgccggt gctggtgacc gaagagggcg aatgctggtt tgattcgccg
     1441 atcatcgctg aatatattga gttaatgaat gtcgctccgg cgatgttgcc gcgcgatccg
     1501 ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg atggcattat ggatgccggg
     1561 ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc agtctgaaga tgaattgtta
     1621 cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg aaggatatct ggtcgatggc
     1681 acactcaaaa ccgatacggt caatctggcg actatcgcca ttgcctgtgc tgtcggatat
     1741 ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc gcccacactt agtcaaactg
     1801 gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg aaccgccaaa ggcttgatgc
     1861 gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg gttatgttaa acgcccttct
     1921 ccgtgtgaga gggccttgat cagccaggtt tcctatgaca accgaaacgc gttccctcta
     1981 tagtcaactt ccggctattg atcgcttatt acgcgatagc tccttccttt ctttgcgtga
     2041 tacttatggt cacacccgcg tggtggaatt gttgcgtcag atgctcgacg aagcgcgaga
     2101 agtgattcgt ggcagccaga cgctgcctgc gtggtgtgaa aactgggcgc aagaagtcga
     2161 tgcccggttg acgaaagagg cgcagagcgc gctgcgtccg gtgatcaacc tgacgggaac
     2221 cgtgctgcat accaaccttg ggcgagcttt acaggcggaa gccgcggtgg aagccgttgc
     2281 gcaggctatg cgttcgccag tgaccctcga gtacgatctg gacgacgccg gacgcggaca
     2341 tcgcgatcgg gcgctggcgc agctgctgtg ccgtattacg ggggcggaag atgcctgtat
     2401 cgtcaataac aatgcggcgg cggtgttatt gatgttggcg gccactgcca gcgggaaaga
     2461 ggtggtggta tctcgcggcg aactggtgga gattggcggc gcgtttcgta ttcccgatgt
     2521 tatgcgtcag gcaggctgca ccctacacga agtagggacc accaaccgca cgcacgcgaa
     2581 tgattatcgt caggcggtga atgaaaatac cgcactgttg atgaaagtac ataccagtaa
     2641 ctacagcatt caggggttca ccaaagcgat agacgaagct gaactggtgg cgctcggcaa
     2701 agagctggat gttccagtgg tgactgattt aggcagtggc tcgctggtcg atcttagcca
     2761 gtacggtttg ccgaaagagc caatgccgca ggagttgatt gcggcgggcg tcagtctggt
     2821 gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca gggattatcg ttggtaaaaa
     2881 agagatgatc gcccgcctgc aaagccaccc gctgaagcgt gcattacgcg cggataaaat
     2941 gaccctcgcg gcgctggaag ccacgttgcg tctttattta caccctgaag ctctgagtga
     3001 aaaattaccg accctgcgcc tgcttacccg cagcgcagag gtcattcaaa tccaggcaca
     3061 acgtttacag gctccccttg tcgcacatta cggcgcggag tttgcggtac aggttatgcc
     3121 atgtctttcg cagattggca gtggttcgct gccggttgat cgcctgccga gcgcggcatt
     3181 aacgtttaca ccccatgatg gacgcggtag ccaccttgag tcattagccg cccgctggcg
     3241 tgaattgcca gtgccggtga ttggtcgtat ttatgacgga cgattgtggc tggatttacg
     3301 ctgccttgaa gatgagcaac ggtttttgga gatgttgttg aaatgattat tgcgactgcc
     3361 ggacacgttg accacggcaa aacaacctta ttgcaggcga ttactggcgt aaatgctgac
     3421 cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc tcggctatgc ctactggccg
     3481 cagccggatg gtcgcgtgcc tggttttatc gacgttcccg gtcatgaaaa gtttctttcc
     3541 aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt tggtggtggc gtgcgatgac
     3601 ggcgtgatgg cacagacccg tgagcatctg gcgattttgc agctgaccgg taacccgatg
     3661 ctgacagtgg cgctgaccaa agccgatcgt gtggacgaag cgcgtgttga tgaggttgaa
     3721 cgccaggtaa aggaggttct gcgggaatac ggttttgctg aggcaaaact gtttatcacc
     3781 gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc atctgcttca gttgccggaa
     3841 cgcgagcacg ccagccaaca tagtttccgc ctcgcgattg accgcgcatt taccgtaaaa
     3901 ggtgccgggc tggtcgtcac cggtacggcg ttaagcgggg aagtgaaggt aggcgattca
     3961 ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag
     4021 ccaacagaaa ccgccaatgc cgggcagcgt atcgcgcttg acatcgcggg tgatgcggaa
     4081 aaagagcaga ttaaccgtgg cgactggctg cttgccgatg tgccgccaga gccgttcaca
     4141 cgggtgattg tcgagcttca aacccatgca ccgctgaccc agtggcagcc gctgcatatt
     4201 caccacgccg ccagccacgt cacgggacgc gtttcactgc tggaagataa ccttgctgaa
     4261 ctggtcttcg acaccccgtt atggctggca gataacgacc gcctggtatt gcgcgatatc
     4321 tctgcccgca acacgctggc cggagcgcgc gtcgtgatgc ttaacccgcc gcgtcgcggt
     4381 aaacgtaaac cagagtatct gcaatggctg gcttccctcg ccagggcaca gaatgatagc
     4441 gaagcgttag ccgttcacct gcaacgcggc gcggttaatc ttcctgattt cgcctgggcg
     4501 cgccagctca acggcgaagg gatgcgcgaa ctgctgcaac agcctggcta tattcaggct
     4561 ggttatagct tgttgaatgc gccggttgcc gctcgctggc aacggaaaat tctcgacaca
     4621 ttagcgactt atcatgagca acatcgcgat gaacctgggc ctgggcgcga acgtctgcga
     4681 cgtatggcgt tgccaatgga agatgaagcg ctagtactgt tgctgattga aaagatgcgc
     4741 gaaagcggcg acatcctcag tcatcacggt tggctgcatc tgccggatca caaagcgggc
     4801 ttcagcgaag agcagcaggc catctggcaa aaagcagagc cgctgtttgg tgacgagccg
     4861 tggtgggtgc gtgatctggc aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg
     4921 ctacgccagg cggcgcagca aggaataatt accgcgatcg tcaaagatcg ttattaccgt
     4981 aacgatcgga ttgtcgaatt tgccaatatg atccgcgatc tcgatcagga gtgtggttca
     5041 acctgcgcgg cggatttccg cgatcgctta ggcgtaggcc gaaagctggc aattcagatt
     5101 ctggaatatt ttgaccgcat tggctttacg cgtcgtcgtg gaaatgatca tttattgcgc
     5161 gacgcattat tatttccgga aagataagga aatgattaat taatttttaa aataaattaa
     5221 tacaaatttc ttatgatttt aaataaagca cattgtttaa tgaatacaat gtgcttttta
     5281 ttagattaat tttgataatt aataataatg atcatgtttt cgaatgaaaa tcctcagtaa
     5341 gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac tttctttatt
     5401 ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat gatggcagat
     5461 tatggattta cccgtacctt aattgtcact gacaatatgt taacgaaatt aggtatggcg
     5521 ggtgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta tgatggcacc
     5581 caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa agaaaataat
     5641 tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa aggtattgcg
     5701 ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg ctctgcaaaa
     5761 ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga aatgacccgt
     5821 ttctgcatca tcactgacga agcgcgtcat aacaaaatgg cgattgttga taagcacgtg
     5881 actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa gtcactgacc
     5941 gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc tattgccgcc
     6001 acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga aaacctgccg
     6061 ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta tgcccagttc
     6121 cttgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat ggcgcaccag
     6181 ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttctgct gccgcatgtt
     6241 caggtattca acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc tgcaatgggc
     6301 gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa cgccatccgt
     6361 gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt gaaagaagaa
     6421 gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggctttac taatccgatc
     6481 caggcaactc acgaagaaat tgtggtgatt tatcgcgccg caatgtaatc atcattccaa
     6541 caacggctgg caaattgtca gccgcttttt caactatctc tgtaatcctt gcccgtaaat
     6601 tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt cacgtctacc
     6661 cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct tcagcacaga
     6721 ttaagcccgg cgagtatggt ttccccatca agttaaaagc ccgctacgac aactttattg
     6781 gcggtgaatg ggtagcccct gccgatggtg agtattacca gaacctgacg ccggtgaccg
     6841 ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg
     6901 ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc gcggcgattc
     6961 tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca gctgaaacct
     7021 gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg gcgattgacc
     7081 atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt gaagttgata
     7141 gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag attatcccgt
     7201 ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg gcgggcaact
     7261 gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta atggaaattg
     7321 tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt ggggaaattg
     7381 gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag
     7441 tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg ctggagttgg
     7501 gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat gcctttttcg
     7561 ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt tgcacctgtc
     7621 cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt tatggaacgc gccatccgcc
     7681 gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg ggcgcgcagg
     7741 tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa aaagagggcg
     7801 ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa gacggctact
     7861 acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag gaggagattt
     7921 ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg gagctggcga
     7981 acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat ctggcctata
     8041 agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac gcttacccgg
     8101 cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc cacaagatga
     8161 tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat aaaccgttgg
     8221 ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg cgacgcttac
     8281 gcgtcttatc atgcctacgg gaacctgacc gtaggccgga taaggcgtac acgccgcatc
     8341 cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg cgcaataacc
     8401 cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg
     8461 tttcgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg aggccaatca
     8521 gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg gatgttttac
     8581 tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa acctcgtcct
     8641 gttgaaaaac cagacagcac atttcagaaa gtatataaaa aacaatatgt cgttatataa
     8701 ataaggaaat tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata
     8761 ttctttgagg gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt
     8821 ctggtgatat tctatgggat tattatttta catgatattc cctacctgat ttctaaaaaa
     8881 cgtaatcatc ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgctg
     8941 catgttatct ggccattttt gtggatttgg gccacgcttt accgcccgga gcggggatgg
     9001 ggaatgcaaa tccatgactc ctccgttgtg caactacaac agcgtattgc cgggctggag
     9061 aaacagctcg ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta
     9121 ttttgaccta tgtggctttt gcatgggcaa tgtttaagat cttcaaaatt cctgtaaata
     9181 aatggaccat ttccacagcg gccctgggtg gcatatttat tgtcagtggt ttaattctgt
     9241 taatgaacta taaccatccg tataccttca aggcgcaaaa agcggttatt tctattcctg
     9301 ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta
     9361 aaaaaggtga ggtgctattt cgactggacc cgacgcgtta tcaggtgcgg gtggatcggc
     9421 tgatggcgga tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg
     9481 agatggcggc gaatactcag caggcaaagg ccacgcggga taaattcgct aaagagtatc
     9541 agcgttacgc acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg
     9601 tggcgcggca aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa
     9661 aacagatcca gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc
     9721 tgaaagcgca gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga
     9781 gcgatggtta tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc
     9841 tacgtccggt gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc
     9901 agaactcctt gctgcgactg gcccctggcg acgatgcgga agtggtgttt aatgctctgc
     9961 caggtaaggt attcagcggc aagctggcag ccattagtcc agctgttccc ggcggagctt
    10021 atcagtcgac cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg
    10081 cgaccattga actggatgag cacactgatt tgagcgcttt accagacggt atttacgccc
    10141 aggtggcggt ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctattac
    10201 gcatgaccag ctgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt
    10261 gttcaggccg cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat
    10321 ccagcacata ctggacccgc cagggcaggt attcccgttt acggaagata gccaccagat
    10381 ccagccacca ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcagatcgc
    10441 tctgcacact tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg
    10501 ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac
    10561 tcccggcgac gttgaagcgc cagatattcg cgtcgtcgat catcatcgag tcgatcaaaa
    10621 tacaggagtg atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct
    10681 ggctggcata agcggttacg gcatattgtg taataaaact ggcaaccagc gattcatctt
    10741 ttggtggagc gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa
    10801 aggcattacg ctcaa
//
LOCUS       DADLZM010000133        10718 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLZM010000133 DADLZM010000000
VERSION     DADLZM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.134"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(107..1771)
                     /gene="glnS"
                     /locus_tag="JNY73_003317"
     CDS             complement(107..1771)
                     /gene="glnS"
                     /locus_tag="JNY73_003317"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY8049220.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1974..3920)
                     /gene="nagE"
                     /locus_tag="JNY73_003318"
     CDS             complement(1974..3920)
                     /gene="nagE"
                     /locus_tag="JNY73_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY8049221.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4253..5053
                     /gene="nagB"
                     /locus_tag="JNY73_003319"
     CDS             4253..5053
                     /gene="nagB"
                     /locus_tag="JNY73_003319"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY8049222.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5113..6261
                     /gene="nagA"
                     /locus_tag="JNY73_003320"
     CDS             5113..6261
                     /gene="nagA"
                     /locus_tag="JNY73_003320"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY8049223.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAGAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPNFKITKTIVNGNEVVTQ"
     gene            6270..7490
                     /gene="nagC"
                     /locus_tag="JNY73_003321"
                     /gene_synonym="nagR"
     CDS             6270..7490
                     /gene="nagC"
                     /locus_tag="JNY73_003321"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY8049224.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7538..8280
                     /gene="nagD"
                     /locus_tag="JNY73_003322"
                     /pseudo
     CDS             7538..8280
                     /gene="nagD"
                     /locus_tag="JNY73_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            8677..10341
                     /gene="asnB"
                     /locus_tag="JNY73_003323"
     CDS             8677..10341
                     /gene="asnB"
                     /locus_tag="JNY73_003323"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY8049225.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYARDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         2671 a         2773 c         2701 g         2573 t
ORIGIN      
        1 aggcctgata agcgaagcgc atcaggcaat ttagcgtttg aaatttattg tctatttatt
       61 gccggatgcg acgtaaatgc ctcacccggc ttggtagaac ttaaaattac tcgcctactt
      121 tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcg gccgtggaat
      181 ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta cccgctaccg
      241 catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc gggttaatca
      301 ccgacaggaa atcatccgca gcacctgggt taggcacgct gaacagacga tcatacaaac
      361 ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct ttgactttac
      421 gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag atggtggtga
      481 tattaccttc ggcatctttc tccacgcgtt cagccttaat gacgtaagca ttacgcagac
      541 gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg cggaaatcgg
      601 cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg gctccccatt tccggtttgt
      661 tcggatggtt cggcatggtg accatttcgc cttcgccctg atagttttcg ataaccagtt
      721 tcaccggatc gataactgcc attgcgcgcg gcgcattttc gttgagatct tcacggatgc
      781 aggattccag cgacgccatc tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc
      841 agaactcacg aatagaagcc gcagtgtaac cacgacgacg cagaccggaa atggtcggca
      901 tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc agcttacgct
      961 tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc ggatgaacag
     1021 gaatggtgat gttatccagt acccagtcgt acagacgacg gttgtcctgg aactcaagcg
     1081 tacacagaga gtgcgtaata ccttccagcg catcgctgat gcaatgggtg aagtcgtaca
     1141 tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta atgcggtaca
     1201 gcaccggatc gcgcattacg ataaatggcg acgccatgtc gattttcgca cgcaggcagg
     1261 ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccaag ttctcttcaa
     1321 cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt cagggtaccg cggtattcgc
     1381 ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc tttattgatc agttcgatcg
     1441 cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca gaccagtgaa
     1501 aacctaacca ctctacgtcg tttttgatcg aatcgacata ctcgatatct tcttttaccg
     1561 ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg atcccgaagt
     1621 tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc ggcgggaaac
     1681 gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc tgacggataa
     1741 agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt aaacaacgta
     1801 taacggcgta tgatcttata agcgggacag gctgacaact gttagttttt taaaattgat
     1861 aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga agatctccac
     1921 cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca ggcatacagc agattacttt
     1981 ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt aatgatcaag
     2041 ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg ggcgttagcg
     2101 ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg cgcaccctct
     2161 tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc catatggacg
     2221 acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt gaagattttc
     2281 acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac cgccacaccg
     2341 tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc cacgacatca
     2401 ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc ctgcggtttt
     2461 gctacaggcg cggcagttgc cggagttgct tcagctgacg cagcggctac cggaccacgg
     2521 gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac aatcacctga
     2581 atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca catcgtatcg
     2641 ttgacgcggg cagagtcagc cacggtcagg cgcagacggg taatacaggc atcaatcgct
     2701 ttcaggttgt cagtaccgcc aaccgcagca atatagttgg ttgccagttg agtcagacct
     2761 tcttcagtat tgctgttggc ttcttcagta acgatctcgt cttctttatc ttcacgaccc
     2821 ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta gatagcgaag
     2881 aagataacgc ccatcaccag cagcatccag acgttctggc tggcggccgg caggttatac
     2941 atcaacgcgt agtcgatagc acccgcagag aaagagaagc cggcatggat acccagcagc
     3001 gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag cagcggcgca
     3061 aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc agtaacagca
     3121 acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc gaagtacatc
     3181 gccagcgccg cacccggcag accgaacatc atgatcggga agaagccgga catgaacatc
     3241 cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc cgtggaaaac cgtacccgcc
     3301 gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg atgcagaccg
     3361 gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc gcccgcagaa
     3421 acgatccact cgccgcctgc atggatagcg tgctgtaccg gcggccagac gtaaccaaaa
     3481 atggccgcca gcaccagaca gaagaagccg gtggcgatcg gcacaaagcg tttgccgccg
     3541 aagaagctca ggaagtccgg cagtttaata tcggaccaac ggttataggc tgcgccacca
     3601 accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt gatgatcacc
     3661 atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc acctgcgctg
     3721 tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt atcaaaaatt
     3781 gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc
     3841 aacagcgccg ccaccggcag caccgcgata gggagctgta acgccctacc gagtcgctgg
     3901 aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc tcgtttaaag
     3961 ccgtattttt attttgctgc aaattttact gccgatgttc tgtaatcaga ttgttagatc
     4021 atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt
     4081 gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaac cagaaaactt
     4141 attttatcat tcaaaaaatc aagtcggatt gacgcctgtc tgcgcaaatc caggttacgc
     4201 ttaaagatgt ctaatccgcc aacggcttac attttactta ttgaggtgaa taatgagact
     4261 gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata tcgtcaatcg
     4321 tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta ctgggcctgc cgactggcgg
     4381 cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt
     4441 taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag agcatccgga
     4501 aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc cagcagaaaa
     4561 catcaacctt ctcaacggca atgccccgga tatcgatgcc gagtgccgcc agtatgaaga
     4621 aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta acgacggtca
     4681 tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca aaaccctgac
     4741 tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc aggtgccaaa
     4801 atatgccctg actgtcggtg ttggtacact gctggatgcc gaagaagtga tgattctggt
     4861 gctgggtagc cagaaagcac tggcgctgca ggccgccgtt gaaggttgcg tgaaccatat
     4921 gtggaccatc agctgtctgc aactgcatcc gaaagcgatc atggtgtgcg atgaaccttc
     4981 caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag cagaaaatat
     5041 caaaggtctg taattgttat ccctgccctg gttccttgct cagggcaata ttttttaaaa
     5101 tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca cgaatttctt
     5161 gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa
     5221 ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc cggttttatc
     5281 gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctgg agcggtcagc
     5341 gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac taactatctg
     5401 ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag
     5461 tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat
     5521 ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc cgcgctggtc
     5581 gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc ggaaatggtt
     5641 cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc cggtcactcc
     5701 aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg ggattacctt tgccacccat
     5761 ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc
     5821 gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga ttacgccaac
     5881 attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga cgccaccgcg
     5941 ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata ctaccgtaac
     6001 ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat gattgaaggc
     6061 gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg tatggcgacg
     6121 ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg gcacgctcgc cgcaggtaaa
     6181 gtagccaacc tgaccgcatt cacacctaat tttaaaatca ccaagaccat cgttaacggt
     6241 aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct cagataggta
     6301 atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt gaccagtacg
     6361 ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc agcgtaacca
     6421 aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag caggcctcca
     6481 ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac gcaatcggcg
     6541 tacggcttgg tcgtcatgac gccaccatca ctctgtttga tctcagcagc aaagtgctgg
     6601 cagaagaaca ttacccgctg ccggaacgta cccagcagac gctggaacat gccctgctga
     6661 atgccattgc tcagtttatt gatagctacc agcgcaagct gcgcgagcta atcgccattt
     6721 cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac atgccgcata
     6781 ttcaggtaga aaactggggg ctggtagaag cactgaaaga acgttttaaa gtgacctgtt
     6841 tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt gcaagtcagg
     6901 attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg attatctcta
     6961 acgggcgcat ttttattggc cgcaacggta acgtcggtga aattggccat attcaggtcg
     7021 aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact atcgccgcca
     7081 acgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag agccgtgtgc
     7141 cactggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc gatagcctgg
     7201 cctcggaagt gattgagtat gtcggtcgtc atctgggtaa aaccatcgcc attgctatca
     7261 acttatttaa tccgcaaaaa attgttattg ctggtgaaat caccgaagcc gataaagtgc
     7321 tgctccctgc tattgaaagc tgcattaata cccaagcgct gaaggcgttt cgtactaatc
     7381 tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt gcgctggtaa
     7441 aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa tgtgctttta
     7501 tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa atgtaatttg
     7561 cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag cggaattttt
     7621 gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc cttcgcagac
     7681 tgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg atagcgtgtt
     7741 ttatacctct gcgatggcga ccgccgattt cctgcgtcgc caggaaggca agaaagcgta
     7801 tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca ctattaccga
     7861 tgtgaaccct gatttcgtga ttgttggcga aacacgttcc tacaactggg acatgatgca
     7921 taaagcagcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc cggacacggt
     7981 ttttatcccg cttgtggcgc gttgtgtgca gggattgaga aaatctccgg gcgcaaaccg
     8041 ttctatgttg gtaagcccag cccgtggatc atccgcgcgg cattaaacaa aatgcaggcg
     8101 cattcggaag aaacagtgat tgtcggcgat aacctgcgta ccgatattct ggcgggcttc
     8161 caggccggtc tggagacgat tctggtgctt tctggtgttt catcgctcga cgatatcgac
     8221 agtatgccgt tccgcccaag ctggatttac ccgtcagtcg ctgaaatcga cgttatctga
     8281 atacccccca cgtctgcgga cgtggttggc tctgatgaca aacgccaaac tgcctgatgc
     8341 gctacgctta tcaggcctac gcagctcctg caatatattg aattttcatg cttttgtagg
     8401 ccgtataagg cgttcacgcc gcatccggca tgaacaaatc gcactttatc agcaatttgg
     8461 cccccgtaaa atataaccat tcaataaaac ccgctaaaaa ttatcgattc atcaataaat
     8521 acatcctcac accagaccct ttttcaccaa tcgacaatca ccattacgtt ttttattttt
     8581 gcactgccaa atagcttgcg cacagtgctg ctttgcggca tttttttaaa caagcaaaca
     8641 caacaagcaa caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc
     8701 gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc acgcctgatg
     8761 cgtcatcgtg gcccggactg gtccggtatt tatgcccgcg ataacgccat tctcgctcac
     8821 gaacgtctgt caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa
     8881 acccacgtac tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg cgctgaatat
     8941 ggcgatcgtt atcagttcca gacagggtct gactgtgaag tgatcctcgc gctgtatcag
     9001 gaaaaagggc cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac
     9061 agcgaaaaag atgcctacct gattggtcgc gaccatctgg ggatcatccc actgtatatg
     9121 gggtatgacg aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct ggtgccagtt
     9181 tgccgcacga ttaaagagtt cccggcgggg agctatttgt ggagtcagga cggcgaaatc
     9241 cgttcttatt atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac
     9301 aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa gccatctgat gtctgatgtg
     9361 ccttacggtg tgctactttc tggtggtctg gattcctcaa ttatttccgc tatcaccaag
     9421 aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccgcagtta
     9481 cactcctttg ctgtaggtct gccgggttca ccggatctta aagcagccca ggaagtggca
     9541 aaccatctgg gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc
     9601 atccgcgacg tgatttacca catcgaaact tatgatgtga ccactattcg cgcttcaaca
     9661 ccgatgtatt taatgtcgcg taagatcaag gcgatgggca ttaaaatggt gctgtccggt
     9721 gaaggttctg atgaagtgtt tggcggttat ctttacttcc ataaagcgcc caacgctaaa
     9781 gaactacatg aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc
     9841 gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa
     9901 ttccttgatg tggcgatgcg cattaacccg caggataaaa tgtgcggtaa cggcaaaatg
     9961 gaaaaacaca tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg
    10021 cagaaagagc agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg
    10081 gcggcgcagc aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac
    10141 acgccgacct caaaagaagc gtatctgtac cgcgagatct ttgaagaact gttcccactt
    10201 ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg cgtgttcttc agctaaagcg
    10261 atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt ctggtcgtgc ggttggcgtt
    10321 caccagtcgg cgtataagta agaaatgcgg tactgccccg gagaaatccg gggctgtttt
    10381 agttattgcg ctgcttgttg attctgatat gcagtaagga aatgttccgg gtttgccaca
    10441 tcactgtttg acaactcatc actgtaggcc agtatttcat agattaattt cccaccacta
    10501 ttgttgaatt ccgaatttca tcctgtacga caaaagaatg gctgtagggt ggggaaaaaa
    10561 cacaaagtaa acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag
    10621 tcactttcga gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa
    10681 tgcgccccgc aacgccgata aggtatcgcg aaaaaaag
//
LOCUS       DADLZM010000134        10698 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.135, whole genome shotgun sequence.
ACCESSION   DADLZM010000134 DADLZM010000000
VERSION     DADLZM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.135"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            126..1823
                     /locus_tag="JNY73_003324"
     CDS             126..1823
                     /locus_tag="JNY73_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY8049226.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="JNY73_003325"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="JNY73_003325"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY8049227.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="JNY73_003326"
     CDS             3113..3397
                     /locus_tag="JNY73_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY8049228.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="JNY73_003327"
     CDS             complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="JNY73_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY8049229.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4093..4977)
                     /gene="fdxH"
                     /locus_tag="JNY73_003328"
     CDS             complement(4093..4977)
                     /gene="fdxH"
                     /locus_tag="JNY73_003328"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY8049230.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="JNY73_003329"
     CDS             complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="JNY73_003329"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7450..7452),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY8049231.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8269..9150
                     /gene="yddG"
                     /locus_tag="JNY73_003330"
     CDS             8269..9150
                     /gene="yddG"
                     /locus_tag="JNY73_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY8049232.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9409..10509
                     /gene="ompC"
                     /locus_tag="JNY73_003331"
     CDS             9409..10509
                     /gene="ompC"
                     /locus_tag="JNY73_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY8049233.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2622 a         2743 c         2654 g         2679 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcac cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcacctt ataaagcgcc
     3421 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3481 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3541 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3601 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3661 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3721 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3781 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     3841 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     3901 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     3961 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4021 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4081 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4141 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4201 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4261 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4321 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4381 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4441 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4501 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4561 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4621 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4681 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4741 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaacaccga cgcagtgccc
     4801 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     4861 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     4921 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     4981 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5041 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5101 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5161 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5221 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5281 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5341 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5401 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5461 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5521 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5581 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5641 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5701 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5761 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     5821 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     5881 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     5941 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6001 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6061 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6121 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6181 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6241 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6301 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6361 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6421 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6481 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6541 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6601 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6661 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6721 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6781 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     6841 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     6901 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     6961 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7021 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7081 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7141 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7201 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7261 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7321 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7381 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7441 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7501 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7561 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7621 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7681 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     7741 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7801 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     7861 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     7921 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     7981 tgttccagcc atacctcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8041 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8101 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8161 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8221 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8281 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8341 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8401 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8461 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8521 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8581 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8641 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8701 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8761 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     8821 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     8881 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     8941 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9001 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9061 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9121 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9181 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9241 atctcatttc gacttcgatc acatatcgat aaataatatt atttcatcta tttttctcat
     9301 tgtttttaat caccattcta ttttaaaaac agtatgtcat ttataaatga cgccgtttaa
     9361 aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
     9421 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
     9481 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
     9541 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
     9601 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
     9661 aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa
     9721 attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta cgacattggt
     9781 tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa agatgtcttc
     9841 atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt tggcttaatt
     9901 gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga caacagtcat
     9961 ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat
    10021 gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac aaatgcgcag
    10081 gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact atgggccaca
    10141 ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga aacattaaac
    10201 atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga agtggtggcg
    10261 caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc gaaaggcaga
    10321 gatctgggcc gctacgccga ccaggacatg attgagtata tcgacgttgg tgcgacgtat
    10381 ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat tgatgaaagc
    10441 gactttaccc gtgccgtaga tattcgcacc gataacatcg tcgctacggg cattacctat
    10501 cagttctaac gtcctctcct gaccagccct tctctgggct ggtttttgat ttaagtgctg
    10561 ctgtatgact ccttaagtaa aaatgagcaa tttttgatat ttctggtcat tcacctcaat
    10621 taatattatt ccatatcctc cggggacatc ttactataat atttattgat gagtgattat
    10681 gaccaatatt aatacagc
//
LOCUS       DADLZM010000135        10612 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.136, whole genome shotgun sequence.
ACCESSION   DADLZM010000135 DADLZM010000000
VERSION     DADLZM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.136"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..2005
                     /gene="speF"
                     /locus_tag="JNY73_003332"
                     /pseudo
     CDS             1..2005
                     /gene="speF"
                     /locus_tag="JNY73_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="JNY73_003333"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="JNY73_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY8049234.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGTSLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNY73_003334"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNY73_003334"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY8049235.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNY73_003335"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNY73_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY8049236.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="JNY73_003336"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="JNY73_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8049237.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6608..6970
                     /gene="ybfE"
                     /locus_tag="JNY73_003337"
     CDS             6608..6970
                     /gene="ybfE"
                     /locus_tag="JNY73_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY8049238.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="JNY73_003338"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="JNY73_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY8049239.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="JNY73_003339"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="JNY73_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY8049240.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="JNY73_003340"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="JNY73_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY8049241.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="JNY73_003341"
     CDS             complement(8459..8785)
                     /locus_tag="JNY73_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049242.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNY73_003342"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNY73_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY8049243.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2569 a         2637 c         2681 g         2725 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatt agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggca cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatcg ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcttg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cttgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgactcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga actgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctgacagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    10321 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    10381 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    10441 cggttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    10501 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    10561 attaattcgc gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg ga
//
LOCUS       DADLZM010000136        10292 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLZM010000136 DADLZM010000000
VERSION     DADLZM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.137"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..401
                     /locus_tag="JNY73_003343"
                     /pseudo
     CDS             1..401
                     /locus_tag="JNY73_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="JNY73_003344"
     CDS             484..741
                     /locus_tag="JNY73_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049244.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JNY73_003345"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JNY73_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAY8049245.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JNY73_003346"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JNY73_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAY8049246.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JNY73_003347"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JNY73_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAY8049247.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JNY73_003348"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JNY73_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAY8049248.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JNY73_003349"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JNY73_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAY8049249.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JNY73_003350"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JNY73_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAY8049250.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /locus_tag="JNY73_003351"
     CDS             complement(8323..8844)
                     /locus_tag="JNY73_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY8049251.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9120..9324
                     /locus_tag="JNY73_003352"
                     /pseudo
     CDS             9120..9324
                     /locus_tag="JNY73_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9318..9422
                     /locus_tag="JNY73_003353"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="JNY73_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="JNY73_003354"
     CDS             9762..10019
                     /locus_tag="JNY73_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAY8049252.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2400 a         2733 c         2822 g         2337 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc ag
//
LOCUS       DADLZM010000137        10205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.138, whole genome shotgun sequence.
ACCESSION   DADLZM010000137 DADLZM010000000
VERSION     DADLZM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.138"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            31..834
                     /gene="hpaI"
                     /locus_tag="JNY73_003355"
     CDS             31..834
                     /gene="hpaI"
                     /locus_tag="JNY73_003355"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY8049253.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALTARFGNSSSISMAKQNNNSVY"
     gene            856..2211
                     /gene="hpaX"
                     /locus_tag="JNY73_003356"
     CDS             856..2211
                     /gene="hpaX"
                     /locus_tag="JNY73_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY8049254.1"
                     /translation="MTTSSLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2221..3111
                     /gene="hpaA"
                     /locus_tag="JNY73_003357"
     CDS             2221..3111
                     /gene="hpaA"
                     /locus_tag="JNY73_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY8049255.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3219..4075
                     /locus_tag="JNY73_003358"
                     /pseudo
     CDS             3219..4075
                     /locus_tag="JNY73_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4302..5860
                     /gene="hpaB"
                     /locus_tag="JNY73_003359"
                     /pseudo
     CDS             4302..5860
                     /gene="hpaB"
                     /locus_tag="JNY73_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5878..6390
                     /locus_tag="JNY73_003360"
     CDS             5878..6390
                     /locus_tag="JNY73_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY8049256.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGESRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6783..8933
                     /gene="btsT"
                     /locus_tag="JNY73_003361"
     CDS             6783..8933
                     /gene="btsT"
                     /locus_tag="JNY73_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY8049257.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8983..9186
                     /locus_tag="JNY73_003362"
     CDS             8983..9186
                     /locus_tag="JNY73_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY8049258.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9197..10153
                     /gene="yjiA"
                     /locus_tag="JNY73_003363"
     CDS             9197..10153
                     /gene="yjiA"
                     /locus_tag="JNY73_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY8049259.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2428 a         2656 c         2643 g         2478 t
ORIGIN      
        1 ttagctgccg ctttgtttaa ggagagaacg atggaaaaca gttttaaagc ggcgctgaaa
       61 gcaggccgtc cgcagattgg attatggctg gggctgagca gcagctacag cgcggagtta
      121 ctggccggac caggattcga ctggttattg atcgacggtg agcacgcgcc gaataacgtg
      181 caaaccgtgc tcacccaact acaggcgatt gcgccctacc ccagccagcc agtggtacgc
      241 ccatcgtgga acgatccggt gcaaatcaaa caactgctgg acgtaggcac acaaacctta
      301 ctggtgccga tggtacaaaa cgccgacgaa gcccgtaaag cggtacgcgc cacccgttat
      361 ccccccgccg gtattcgcgg tgtgggcagt gcgctggctc gcgcctcgcg ctggaatcgc
      421 attcctgatt acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg
      481 cgtgaggcaa tgaagaactt accgcagatt ctggacgtgg aaggggtcga cggcgtgttt
      541 atcggcccgg cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa
      601 gtacaggccg ccattgagca ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg
      661 atcctgatcg ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc
      721 gccgtcggcg ttgacaccac cctgctcgcc cgcgccgccg aagcgctgac agcacggttt
      781 ggtaatagtt cttctatttc gatggcgaag caaaacaaca actctgtcta ctaaacgtac
      841 gatgtggagc gcatcatgac gacctctagc ctgcaatata aagataataa aactgttgaa
      901 attgaacatc gtgtcattaa taaattattt cgtcgcctaa tcatttttct ttttattctg
      961 tttgtttttt cgtttcttga tcgcatcaat ataggatttg ccggactgac aatgggcaag
     1021 gatcttggcc tgacgtccac aatgtttggc ctcgccgcga cactctttta tgtcacgtac
     1081 gtcatgtgcg ggatccccag taatatgatg ttagcgaaaa ttggcgctcg tcgatggatc
     1141 gctgggatca tggtggtctg gggaattgct tccacctgca caatgtttgc aacaagcccc
     1201 gaaacactct atctactgcg tatgctggtt ggtattgctg aggcgggttt tttacctggt
     1261 attttggttt atctgacctg gtggttcccg gcatatcatc gggccagagc caatgcctta
     1321 tttatgatag cgatgccagt cactatgatg ctgggattga tcctttcagg ctacattctg
     1381 gccttggatg gactgtggaa tctaaaaggc tggcaatggc tctttttgct ggaaggtctg
     1441 ccatcggtca ttcttggtgt cgtcacctgg ttttatctca acgacacacc agaccaggcg
     1501 acctggcttg atgaaaacga aaaacaggct ttaaaaacga tgattctccg tgaacaagag
     1561 agtgcatttg cccatgcatc tgttccacgc tcaaccttac gtgaagtcct gaccccagcc
     1621 atcatgctat atacacttgc ttatttttgc ctgactaata ctctgagtgc gattaatatc
     1681 tggacgccgc aaatcctaca gagtttcaat actggcagta gcaacatcat gattggtttg
     1741 ctggcggcca tcccccaatt ctgcacaatt tttggcatga tctggtggag tcgtcgttcc
     1801 gaccgtctta aagaacgcaa aaagcatacg attcttccct acctgtttgc cgctgccgga
     1861 tggttactcg cttctgcgac agatcacagc ctcattcaac tgagcggcat cattatggcc
     1921 tcaatgggat catttaccgc gatgactatc ttttggacaa cacaggatca agttataagc
     1981 ttgcaatcgc gtgccgttgc gatggctgtc atcaatgctg tcggcaatgt aggttcagca
     2041 gtcagtccat tacttatcgg tattttgaaa gatgcaaccg gcagttttag ctcaggtttg
     2101 tggtttgtag caggattgct ggtactgggc gcgttagtca tgacccgaat ccctatgggg
     2161 agtcgggaac actctctttc atctgaactt atcgcaccaa aaccacaata aggagtcatg
     2221 atgtccagtg aagctatctt tgaacatggt tatgaaaata ttgatttggt ccgggaatat
     2281 gatgcccgct acgctggtga agatgtgcat tatgaaacat ttacccggtt agcgaatttc
     2341 tttggacgcg atatgcgccc tcactggcac gatagatatt ttcaactcca ttatctggtc
     2401 actggacgca ttactttaca actggatgag cacttctatt ctgtgcgcgc accgcttttt
     2461 gtactaacac caccatcggt gccacatact ttctttactg atcctgatag cgatgggcat
     2521 gttctgaccg tacgtcagga gttaatttgg cccctggtag aaaagctctg gcctggtagt
     2581 ggagatgccg caatgctaca aggtatctgt ctgtcaccgg agggaatgga acatacactg
     2641 gcggcattaa atcattattg gccgataata tccctggagt ttcaacaaaa taagccagga
     2701 cgggaaattc tattgcaatc actggcgcaa agtattttta caatcctact gcgtgaagca
     2761 ccaccaaatg atatttcaac atgcagcgtg cgcggcgaga tgtgtttatt ccagaagttt
     2821 aatcggctga ttgacgaaaa ttatcgccaa catttaatgg tccctgaata tgcaacaatg
     2881 ctcggtatga gtgaatcacg gcttaccgag ctgtgccgac gttttgccaa tcaatcgccg
     2941 aagagattga ttttcgaacg agtgagccgg gaagccagac ggcttctgct ctatagcgag
     3001 caaagcatca accagatagc ccttgatctt ggctacaaag accccgccta tttcgcccgt
     3061 tttttcaacc gaatgatggg ttgctcacca acacaattcc gtggtcgctg accatttatc
     3121 aacctgagag actcgacatg aaaatatctt tacgctcact atttgtggcg tgcctgattg
     3181 cgccagtggc attcacctac gcctccgaac cgcaataaat gaaaattgta ctacttggaa
     3241 cgaaaggtgg cccttcttta ttaaaagctg accgtttacc gatggcaacg gcacttatta
     3301 ttggcaataa aacctggtta attgatgctg gttacggggc cagcttacaa ctggtaaaaa
     3361 ataatattcc actgcgccag attgataata ttttactcac ccatttacac agtgatcata
     3421 ttctggacta tccatcagtc ttaatgaatg catgggctag cggtctgcag gacaaaacta
     3481 ttcatgtatt cggtccgcca ggcacccgag ccatgaccgc tgccagttgg caggtcttcg
     3541 atcgggacat cacactgcgt atggaagaag aaggaaaacc cgatcctcgc aaactcgtta
     3601 cagctcaaga tgttggtgaa ggcgtagttt ataaagatga taccgtgacc gtgactgcat
     3661 taaaggtccc tcattctcct ttccctgacg gtgaggcttt tgcttatcgc ttcgacacac
     3721 aaggcaagcg aattgtcttc tcaggagata cctcgtactt tcccccactg gcaactttct
     3781 cagaaggcgc tgatatactt gtgcatgagg ccgtacatga gccctccgtc gctaagctgg
     3841 cagacagtat tggcaatggt aaaacactgg caaaagccat tgcttctcac catacgacta
     3901 ttgaggatgt aggcaaaatt gccaatgaag caaaagtgaa aaaactcatc cttagccacc
     3961 tggtaccaat caccgttccg gatgaaacat ggaagcaaga agcacagaaa actattcagg
     4021 ccctatcgtc gttggtcatg aaaatatgac gattaccatt ccattcgccc cataaaaaat
     4081 tccgtccccg ttagcccttg ttagcgggga atattatgat ttgccttact gtaagttgat
     4141 cacgatcgca gtttactatt taactccacc ttgctgtcga aaagtaccaa cgttcgcctg
     4201 aaccgtctct tcccgacaac cgcgttcaac gcttcaatta aataacaaaa acaacacaca
     4261 aaattaacaa tcgtattccg attaatactg tagaggtcga catgaaacca gaagatttcc
     4321 gcgccagtac ccaacgtcct ttcaccgggg aagagtatct gaaaagcctg caggatggtc
     4381 gcgagatcta tatctatggc gagcgagtga aagacgtcac cactcatccg gcatttcgta
     4441 atgcggcagc gtctgttgcc caactgtacg acgcgctgca caaaccggag atgcaggact
     4501 ctctgtgttg gaacaccgac accggcagcg gcggctatac ccataaattc ttccgcgtgg
     4561 cgaaaagtgc cgacgacctg cgccagcaac gcgacgccat cgctgaatgg tcacgcctga
     4621 gctatggctg gatgggccgt accccagact acaaagccgc tttcggttgc gcactgggcg
     4681 cgaatccggg cttttacggt cagttcgagc agaacgcccg taactggtat acccgtattc
     4741 aggaaactgg cctctacttt aaccacgcga ttgttaaccc accgatcgtc atttgccgac
     4801 cgataaagtg aaagacgttt acatcaagct ggaaaaagag actgacgccg ggattatcgt
     4861 cagcggtgcg aaagtggttg ccaccaactc ggcgctgact cactacaaca tgattggctt
     4921 cggctcggca caagtgatgg gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat
     4981 ggatgccgat ggcgtgaaat taatctcccg cgcctcttat gagatggtcg cgggtgctac
     5041 cggctcgcca tacgactacc cgctctccag ccgcttcgat gagaacgatg cgattctggt
     5101 gatggataac gtactgatcc catgggaaaa cgtgctgatc taccgcgatt ttgatcgctg
     5161 ccgtcgctgg acgatggaag gcggtttcgc ccgtatgtat ccgctgcaag cctgtgtgcg
     5221 cctggcagtg aaactcgact tcattacggc actgctgaaa aaatcactcg aatgtaccgg
     5281 caccctggag ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt ggcgcaacac
     5341 cttctgggca ttgagtgact cgatgtgttc agaagcgacg ccgtgggtca acggggctta
     5401 tttaccggat catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa
     5461 gatcaaaaac attatagaac gcaacgttac cagtggcctg atctacctcc cttccagtgc
     5521 ccgtgacctg aacaatccgc agatcgacca gtatctggcg aagtatgtgc gcggttcgaa
     5581 cggtatggat cacgtccagc gcatcaagat cctcaaactg atgtgggacg ccattggcag
     5641 cgaatttggt ggtcgtcacg agctgtatga aatcaactac tccggtagcc aggatgagat
     5701 tcgcctgcaa tgtctgcgcc aggcacaaag ctccggcaat atggacaaga tgatggcgat
     5761 ggttgatcgc tgcctgtcgg aatacgacca gaacggctgg actgtgccgc acctgcacaa
     5821 caacgacgat atcaacatgc tggataagct gctgaaataa cgcagcagga ggttaagatg
     5881 caattagatg aacaacgcct gcgctttcat gacgcgatgg tcagcctgtc ggcagcggta
     5941 aatattatca ccaccgaggg cgacgccgga caatgcggga ttacggcaac ggctgtctgc
     6001 tcggtcacgg atacaccacc gtcgctgatg gtgtgcatta acgccaacag tgcgatgaac
     6061 ccggtttttc agggcaacgg caagttgtgc gtcaacgtcc tcaaccatga gcaggaactg
     6121 atggcgcgcc acttcgcggg catgacaggc atggcgatgg aagagcgttt tagcctctca
     6181 tgctggcaaa aaggtccgct ggcgcagccg gtgctaaaag gttcgctggc cagtcttgaa
     6241 ggagagagcc gcgatgtgca ggcgattggc acacatctgg tgtatctggt ggagattaaa
     6301 aacatcatcc tcagtgcaga aggtcacgga cttatttact ttaaacgccg tttccatccg
     6361 gtgatgctgg aaatggaagc tgcgatttaa gctccagccc cttcacgcat tcgtcgaagg
     6421 ggcattttta ttgtgatctc aatcacatat ctacccgatg aataattctt gcccaaccgc
     6481 tcaccgccat tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt
     6541 tacgcgcacg tatctctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac
     6601 gcaacgcaaa cctagaacgg cttcggccta tgattaacaa attactctat ggattttggt
     6661 ttgcaggaag gcagcaagtg agtgaatccc cgggagctta caaaagtaag tgtcagaggt
     6721 gaacgaacgc agctgccgca cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta
     6781 ctatggatac gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg
     6841 cattctgcct cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg
     6901 tggtcgcctc tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc
     6961 agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc
     7021 tgaactacgt tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg
     7081 gtgctggtcc gctggtggga ccggttctcg ccgcgcagat gggctacctg cctggcacgc
     7141 tgtggctgct ggcgggggta gtgctggccg gtgcggtaca ggactttatg gtgctgttta
     7201 tctcctctcg ccgtaacggc gcatctctcg gtgagatgat caaagaagag atggggccag
     7261 taccggggac tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgttc
     7321 tggcgctgat tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc accgtttgct
     7381 caaccgtacc gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg
     7441 tgggtgaagt ctctgttatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg
     7501 tgattgctca cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct
     7561 tcgcgctgat tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac
     7621 cgcgcgacta tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta
     7681 tcgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg
     7741 gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg
     7801 tatctggctt ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa
     7861 ccgacgcgcg tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg
     7921 cgctggttgc agcgtccatc atcgaaccgg gtctttactt cgcgatgaac accccgcctg
     7981 ctggccttgg catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca
     8041 tcatggcgca gctgaaagac gttaccgcac acgcggcagc gaccgtcagc tcctggggct
     8101 tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc
     8161 tgaaccgtgc aggtggtgcg ccgacgctgg cggtaggtat cgctcacgtg ttccacaaag
     8221 tgctgccgat ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt
     8281 tcatcctgac cgcgctggat gcgggtaccc gttctggccg ctttatgctg caagacctgc
     8341 tgggtaactt catcccgttc ctgaaaaaaa ccgactctct ggttgccggt atcatcggta
     8401 ctgcgggctg tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg
     8461 gcggcgttaa gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag
     8521 cgctggtact gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa
     8581 ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt
     8641 tcagcaccaa cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga
     8701 ttgctaacgg tactgacctg acggcgcagc agattgccaa catgaaccac atcgttgtga
     8761 acaactacac caacgcaggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct
     8821 tctacggttt caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa
     8881 caccgtacgt tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtgtt
     8941 tagccccgct tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga
     9001 caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggtattcc agactacgac
     9061 aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa
     9121 ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt
     9181 tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc
     9241 gggcaaaact accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt
     9301 gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac
     9361 gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggagga
     9421 cgcactactc gacctgctgg ataatctcga caagggcaat attcagttcg atcgtctggt
     9481 gattgaatgc accggcatgg ccgatcccgg cccgattatt cagacctttt tctcccatga
     9541 gattttatgc cagcgctacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc
     9601 cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gtgggctacg ccgaccgtat
     9661 tctgctgact aaaaccgacg tcgcaggcga agcagagaaa ctgcgtgaac gcctggcgcg
     9721 catcaacgcc cgcgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct
     9781 gttcaacacc aacggtttta tgctggaaga gaacgtcgtc agcaccaaac cgcgtttcca
     9841 ctttatcgcg gataaacaaa acgatatttc gtcaattgtg gtggaactgg attacccggt
     9901 agatatcagc gaagtttccc gcgtgatgga aaacctgctg ctggaatcgg cggataaact
     9961 gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg
    10021 cgtccagcgc ctctacagcg ccgactggga caggccgtgg ggcgatgaaa aaccgcatag
    10081 cacaatggtg tttatcggga ttcagttgcc ggaggaagag attcgggcgg cgtttgcggg
    10141 attgaggaag taagttcggg aaagatcaac tttttgagag ttccaccgca acattattac
    10201 gagtg
//
LOCUS       DADLZM010000138        10073 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLZM010000138 DADLZM010000000
VERSION     DADLZM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.139"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(296..1156)
                     /locus_tag="JNY73_003364"
     CDS             complement(296..1156)
                     /locus_tag="JNY73_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAY8049260.1"
                     /translation="MALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFD
                     LNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARA
                     AREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQPSTITMISDW
                     LAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADILSPVKETGTLLGVVS
                     SQAAELCGLKAGTPVVVGGGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQ
                     LPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JNY73_003365"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JNY73_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAY8049261.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="JNY73_003366"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="JNY73_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAY8049262.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAGTERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAEDNATPERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="JNY73_003367"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="JNY73_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAY8049263.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="JNY73_003368"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="JNY73_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY8049264.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWFWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="JNY73_003369"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="JNY73_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..7965
                     /gene="lsrF"
                     /locus_tag="JNY73_003370"
     CDS             7090..7965
                     /gene="lsrF"
                     /locus_tag="JNY73_003370"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY8049265.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7989..8279
                     /gene="lsrG"
                     /locus_tag="JNY73_003371"
     CDS             7989..8279
                     /gene="lsrG"
                     /locus_tag="JNY73_003371"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY8049266.1"
                     /translation="MHVTLVEINVHEDKVEEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8318..9076
                     /gene="tam"
                     /locus_tag="JNY73_003372"
     CDS             8318..9076
                     /gene="tam"
                     /locus_tag="JNY73_003372"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY8049267.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9080..9994)
                     /locus_tag="JNY73_003373"
     CDS             complement(9080..9994)
                     /locus_tag="JNY73_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049268.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTYISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         2431 a         2482 c         2653 g         2507 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatggctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt caagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgttctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 ggaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcgg aagataatgc caccccggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tagaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgc ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atccccaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggt tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgctggattg gggaatgcag
     7201 tcacgcttat cgtggatatt taatccgaaa acgggtaaaa ctgtgatgct ggcctttgac
     7261 catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa tatcgccccg
     7321 ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag cgtagttccc
     7381 ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg cgaactctat tctggcggaa
     7441 ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg
     7501 gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa aaatattatt
     7561 cagctggtcg atgccggaat gaaagtggga atgccgacca tggccgtgac tggcgtgggc
     7621 aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg
     7681 ggggcgcaaa ttatcaaaac ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga
     7741 tgtccggtac ccattgttat tgctggcggt aaaaaattac cggagcgcga ggcgctggaa
     7801 atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg taatattttc
     7861 cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggttca ccataacgaa
     7921 acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg gatttaagga
     7981 gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagacaag gttgaagagt
     8041 ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggt aatttgcgct
     8101 tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatttacgaa gcctataaag
     8161 atgaagacac agtggcattc cataaaacca cgcctcacta caaaacctgt gtcgcgaaac
     8221 tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     8281 gctacaattg gggtaacgcg ctgacgggag taaaaaaatg tctgactgga acccctcttt
     8341 atatctgcac ttttccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc
     8401 gctggagaat gtcgaatatg ttgccgatct tggctgtggc ccaggtaaca gcaccgccct
     8461 gctgcatcaa cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat
     8521 tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg gaagcagaca tccgcaactg
     8581 gcaaccggaa caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga
     8641 ccactacgaa ttgtttcctc acttggtttc tttacttaat ccgcagggcg tgctggcagt
     8701 acagatgcca gataactggc tggagccgac ccatgtgctc atgcgcgaag ttgcttggga
     8761 acaaaactac ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt actacgatat
     8821 tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc acttactatc accagatgcc
     8881 gtcgcaccag gcgattatcg attgggtgac cgccactgga ttacgtccgt ggttacagga
     8941 tctgaccgag agcgaacagc agctttttct tacgcgctac catcagatgc tggaagagca
     9001 gtatccactg caagagaacg gacagatact gctggcattt ccgcgtctgt ttattgttgc
     9061 ccgccgtacg gagtaattat cacgtcagct ggtaatgacg atcgggaaga atttttgctg
     9121 gaatttctgc ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat
     9181 ccagcggtaa atcattgttt tcagtaccga acggatcttc cagttcttcc gccagacaat
     9241 ccagcgaaat ataagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt
     9301 ccacgaccag cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca
     9361 gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg
     9421 aaatatcatt aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgtccattgc
     9481 ggcgctgaac cgccaaccat tctcccatta ttaacaagat acggttagct ggagagttcg
     9541 aagccagtac acgctgaaga tctccagtct tgagataatg agccagcact tccgcctgtg
     9601 gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc ctggcaaact
     9661 cccttacact tgccgaatcc ggcaatgtcg tttttacctc tcgcagtaac gaccgtgagg
     9721 caatcatcaa ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat
     9781 tacgaaaacc aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga
     9841 atttgatgcc cagatgcgtg taccagggca gcatgaatat aacagcaata gaaaagagaa
     9901 aattgagtag taagcgcgag gatatcttgg ataatactga gccgtgccag acaaaaatac
     9961 ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg
    10021 ccctattaaa cgtgattacg atcacattct caagacgcta cttacaaatt acc
//
LOCUS       DADLZM010000139         9957 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLZM010000139 DADLZM010000000
VERSION     DADLZM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.140"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..338)
                     /locus_tag="JNY73_003374"
     CDS             complement(<1..338)
                     /locus_tag="JNY73_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY8049269.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="JNY73_003375"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="JNY73_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="JNY73_003376"
     CDS             complement(1132..1338)
                     /locus_tag="JNY73_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY8049270.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="JNY73_003377"
     CDS             complement(1445..2008)
                     /locus_tag="JNY73_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY8049271.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="JNY73_003378"
     CDS             complement(3383..5218)
                     /locus_tag="JNY73_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049272.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="JNY73_003379"
     CDS             5319..5606
                     /locus_tag="JNY73_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY8049273.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="JNY73_003380"
     CDS             5578..7107
                     /locus_tag="JNY73_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAY8049274.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="JNY73_003381"
     CDS             complement(7277..8494)
                     /locus_tag="JNY73_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8049275.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="JNY73_003382"
     CDS             complement(8861..9847)
                     /locus_tag="JNY73_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049276.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT         3138 a         2263 c         1844 g         2712 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg cggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagataga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg taccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcaacg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacaaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttc
//
LOCUS       DADLZM010000140         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLZM010000140 DADLZM010000000
VERSION     DADLZM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.141"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNY73_003383"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNY73_003383"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY8049277.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNY73_003384"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNY73_003384"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY8049278.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="JNY73_003385"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="JNY73_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="JNY73_003386"
     CDS             3456..5216
                     /locus_tag="JNY73_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY8049279.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="JNY73_003387"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="JNY73_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY8049280.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="JNY73_003388"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="JNY73_003388"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY8049281.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRISTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="JNY73_003389"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="JNY73_003389"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY8049282.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2551 a         2495 c         2377 g         2532 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccaag
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttgggt aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caacctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt atctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcattctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DADLZM010000141         9851 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLZM010000141 DADLZM010000000
VERSION     DADLZM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.142"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..123)
                     /locus_tag="JNY73_003390"
     CDS             complement(<1..123)
                     /locus_tag="JNY73_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8049283.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRT"
     gene            555..1673
                     /locus_tag="JNY73_003391"
     CDS             555..1673
                     /locus_tag="JNY73_003391"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY8049284.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1739..1987
                     /locus_tag="JNY73_003392"
     CDS             1739..1987
                     /locus_tag="JNY73_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY8049285.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2052..2420
                     /locus_tag="JNY73_003393"
     CDS             2052..2420
                     /locus_tag="JNY73_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY8049286.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2514..3167
                     /gene="nfsB"
                     /locus_tag="JNY73_003394"
     CDS             2514..3167
                     /gene="nfsB"
                     /locus_tag="JNY73_003394"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY8049287.1"
                     /translation="MNIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3275..4522
                     /locus_tag="JNY73_003395"
     CDS             3275..4522
                     /locus_tag="JNY73_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY8049288.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTKAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4590..5966)
                     /gene="pheP"
                     /locus_tag="JNY73_003396"
     CDS             complement(4590..5966)
                     /gene="pheP"
                     /locus_tag="JNY73_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY8049289.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGDAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGRNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6067..9202)
                     /gene="cusA"
                     /locus_tag="JNY73_003397"
                     /pseudo
     CDS             complement(6067..9202)
                     /gene="cusA"
                     /locus_tag="JNY73_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9214..9755)
                     /locus_tag="JNY73_003398"
                     /pseudo
     CDS             complement(9214..9755)
                     /locus_tag="JNY73_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2419 a         2720 c         2383 g         2329 t
ORIGIN      
        1 tgtcctgacc tgggttcggg ggacaccaaa acgtgccgag atgatcctgt aaccatcatc
       61 agtagtgaag tagtgattca cgacttcaag gcgcttttca aaagggtatt ttggctttga
      121 catattaggg gctattccat ttcatcgtcc aacaaaatgg gtgcagtaca gggttgatag
      181 aaatatctcc tggggcttca tctttccaga cctcagagta aacctgaaat cggaaagctt
      241 caggcacccg cagttatctt acccttcctc aacaaaatag ctgtttattt atacagcaaa
      301 tcctgtttcg gacttctcac tccccatttg atcaactgaa gctgaacccg tttttttctg
      361 catgcctcaa tcgtcaaaat tcttcgccac cagccagctc tgccataact tgccacattg
      421 aaaaagtagc caaagtgctt caggcaccat cagttcacca tcaccaaaaa aaagaaacat
      481 tccagaactt ccgcatctcg cccagccgct ccgacttcta tactgaatag aaaacgtcaa
      541 cttaagagaa acctatgcca ttacccgatt ttcatttttc tgaacctttt accctcggta
      601 ttgaactgga aatgcaggtg gttaatccgc cgggctatga cttaagccag gactcttcaa
      661 tgctgattga cgcggttaaa aataagatca cggccggaga ggtaaagcac gatatcaccg
      721 aaagtatgct ggagctggcg acggatgttt gccgtgatat caaccaggct gccgggcagt
      781 tttcagcgat gcagaaagtc gtattgcagg cagccgcaga ccatcatctg gaaatttgcg
      841 gcggtggcac gcccccgttt cagaaatggc agcgtcagga ggtatgcgac aacgaacgct
      901 atcaacgaac gctggaaaac tttggctatc tcatccagca ggcgaccgtt tttggtcagc
      961 atgtccatgt tggctgtgcc agtggcgatg acgccattta tttgctgcac ggcttgtcac
     1021 ggtttgtgcc gcattttatt gccctttccg ccgcgtcgcc atatatgcag ggaacggata
     1081 cgcgttttgc cccctcacga ccgaatattt tttccgcctt tcctgataat ggcccgatgc
     1141 cgtgggtcag taactggcaa caatttgaag ccctgtttcg ctgtctgagt tacaccacga
     1201 tgatcgacag cattaaagat ctgcactggg atattcgccc cagtcctcat tttggcacgg
     1261 tggaggttcg ggtgatggat accccgttaa cccttagcca cgcggtgaat atggcgggat
     1321 taattcaggc caccgcccac tggttactga cggaacgccc gttcaaacat caggagaaag
     1381 attacctgct gtataaattc aaccgtttcc aggcctgtcg ctatggtctg gaaggcgtca
     1441 ttaccgatcc gcacactggc gatcgtcgac cgctaacgga agataccttg cgattgctgg
     1501 aaaaaatcgc cccttcagca cataaaattg gcgcatcgag cgcaattgaa gcactgcatc
     1561 gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg cgatttcgtc gccgatggcg
     1621 gctcgctgat tgggctggtg aaaaagcatt gtgagatctg ggccggtgac taagccggga
     1681 attgccttac agaagaatat ccctgacaat ggtgttttaa cttacattta acaatgttat
     1741 gaaacaccct ttagaaacct tgaccaccgc agcaggcatt ttgctgatgg ctttcctctc
     1801 ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg gcacaaaaac tggtgaccat
     1861 gttccatctg atggatctta gccagcttta cactttattg ttttgtctgt ggtttttagt
     1921 gctgggcgct attgagtatt ttgttctgcg ctttatctgg cgacgctggt tctcgctggc
     1981 ggattaaacg tcgattatcg ccgaacttcg cggcatactt tgcttattct ccttcgccta
     2041 aaggaacgtt tatggataag caatcactgc acgaaacggc gaaacgccta gctcttgagt
     2101 taccctttgt cgagctttgc tggccttttg gcccggagtt cgatgttttt aaaattggcg
     2161 gcaagatttt tatgctgtcg tcggagctac gcggcgtccc ctttatcaat ctgaagtccg
     2221 atccacaaaa atccctgtta aatcagcaaa tatacccaag cattaagcca gggtatcaca
     2281 tgaataaaaa gcactggatt tcggtgtatt ccggcgagga aatctccgaa gcgttacttc
     2341 gcgatctgat caacgattcg tggaatctgg tggttgatgg tctggctaaa cgcgatcaaa
     2401 aaagagtgcg tccaggctaa agcgggaatc tatagcgcat ttttctcgct taccatttct
     2461 cgttgaacct tgtaatctgc tggcacgcaa aattacttac acatggagtc tttatgaata
     2521 tcatttctgt cgccttaaag cgtcattcca ctaaggcatt tgatgccagc aaaaaactta
     2581 ccccggaaca ggccgagcag atcaaaacgc tactgcaata cagcccatcc agcaccaact
     2641 cccagccgtg gcattttatt gttgccagca cggaagaagg taaagcgcgt gttgccaaat
     2701 ccgctgccgg taattatgtg ttcaacaaac gtaaaatact tgatgcctcg cacgtcgtgg
     2761 tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa gctggttgtt gaccaggaag
     2821 atgccgatgg ccgctttgcc acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt
     2881 tcttcgccga tatgcaccgt aaagatctgc atgatgatgc agagtggatg gcaaaacagg
     2941 tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc gctgggtctg gacgcggtgc
     3001 ccatcgaagg ttttgacgcc gcaatcctcg atgcagaatt tggtctgaaa gagaaaggct
     3061 acaccagtct ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt aacgctacgc
     3121 tgccgaaatc tcgtctgccg caaaacacca ccttaaccga agtgtaattc tctcttgccg
     3181 ggcatctgcc cggctatttc ccctcagatt ctcctgattt gcataaccct gtttcagccg
     3241 tcatcatagg ctgctgttgt ataaaggaga cgttatgcag gatttaatat cccaggttga
     3301 agatttagcg ggtattgaga tcgatcacac cacctcgatg gtgatgattt tcggtattat
     3361 ttttctgacc gccgtcgtgg tgcatattat tttgcattgg gtggtactgc ggaccttcga
     3421 aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc attacccaga ataaactctt
     3481 ccaccgttta gcttttaccc tgcaggggat tatcgtcaat attcaggcgg tattctggct
     3541 gcaaaaaggc accaaagcgg cagatattct gactacctgc gcgcagttgt ggatcatgat
     3601 gtatgcgctg ctttcagtct tctcgttgct ggatgttatt ttgaatctgg cgcagaaatt
     3661 cccggcggca tctcagttac cgctgaaagg gatatttcag gggattaaac tgatcggcgc
     3721 aatcctggtc ggcattttga tgatctcgct gctgattggt cagtcgccag cgattctgat
     3781 cagcggtctt ggtgcaatgg ctgccgtgct gatgttggta tttaaagatc cgattcttgg
     3841 tctggtggca ggtattcagc tttccgcgaa cgatatgctg aaactgggcg actggctgga
     3901 aatgccgaaa tacggcgcgg atggcgcggt gatcgatatt gggttaacca ccgtcaaagt
     3961 gcgtaactgg gacaatacca ttaccactat tcccacctgg tctctggttt ctgactcctt
     4021 taaaaactgg agcgggatgt cagcatctgg cgggcgacgt attaagcgca gtatcagtat
     4081 tgatgtcacc agtattcgtt ttcttgatga agacgaaatg caacgtctga ataaagcgca
     4141 tttgttaaag ccttatttaa ccagccgcca tcaggaaatt aatgagtgga atcgccagca
     4201 aggttctacg gagtcggtat taaacctgcg ccgaatgacc aatattggaa cctttcgtgc
     4261 ctatctgaac gaatatctgc gtaaccatcc gcggattcgt aaagatatga ccttaatggt
     4321 acgccaactg gctccaggtg ataacggttt accgctcgag atctacgcgt ttactaacac
     4381 cgtggtgtgg ctggaatatg aaagcattca ggctgatata ttcgatcaca tatttgcgat
     4441 tgtcgaagag tttggtctgc gacttcatca gtcgccaacc ggcaatgata ttcgctctct
     4501 ggcgggtgca tttaagcagt aattaaaaaa accgctctca tcgaatggat gagagcggac
     4561 atcgttttgt cggatgtagc gtgaatgcct tatttccgac gcagcgtttt aaatgccaca
     4621 aacaggaata caatccacac cggcagcagg atcgctgaca agcgcatatc atccatcgtg
     4681 cacatcagca gcaaaatcat gccgaggaag gcgatgcaaa gatagttgcc gaacggatag
     4741 agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca tcgccgcgcg aaaacgcaga
     4801 tgcgccaggc agatcataat ccagttcaac agcaacgttg ctaccaccag cgtcatcagc
     4861 agaccaaacg ctttttgcgg cagcagatag ttgattaaca ccaccagcga agtgatcgct
     4921 ccggaaagca tcagcgagtt aatcggcaca ccgcgacggc tgacgcgagt caaaaacttc
     4981 ggcgcattac cctgcacaga aaggccaaac agcatgcggc tgttagagta aaccccgctg
     5041 ttatacaccg acagcgatgc caccagaatg acgaagttca gcgcagaagc taccacattg
     5101 ctgtcgagat tatggaaaat catcacaaac gggctactgt tggatttcac ttccacccac
     5161 ggatagagtg ccagtaaaac caccagtgaa ccgatataaa acagcaggat tcgatacacc
     5221 acctgattta ccgcttttgg aatgcttttt tccggatcgc gcgcttcagc ggctgtaatc
     5281 ccaatcagct ccagaccgcc gaaggagaac ataattaccg ccagcgacaa aatcagccca
     5341 ttccggccgg tggcgaagaa accaccgtag cgccagaggt tgtcgatact ggctttctcg
     5401 ccgccgtgac cagaaaacag cagccacagg ccaaagccga tcataccgat gatcgccagc
     5461 actttaatca gcgcaaacca gaactcggtt tcgccatata agcgcacgtt caccaggtta
     5521 acggcgttga tgataataaa gaaggcggca gcccaaatcc acgttggaac atccgggaac
     5581 cagtactgca tatagatgcc cgcagcggtc agctctgcca ttcccaccag cacgaacatt
     5641 acccagtagt tccagccaga gaggaagccc gcaaacggtc cccagtattt ataggcaaag
     5701 tgggcaaatg aaccggatac cggctcttcg accaccatct cgccaagctg gcgcataatc
     5761 aggaaagcga tgatcccggc gacgccgtag cccagcaata cagccggacc cgccatctga
     5821 atcgccgggc caatgccaag aaacagacca gtaccaattg cgtcacccag cgcaatcagt
     5881 tgaatatgac ggttatgtaa tccgcgatga agcgtcggct cttgattcga cgcagtatct
     5941 tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg tgtctttttt gttgaggggc
     6001 accttttaac atttagtgcc catcgtcgca agacacaatc cacacggtta aacgaggtat
     6061 cctgctttaa ttccgtaccc gatgtcggtg cagccacatc agcttatacg ccgccgggat
     6121 aataaacagc gacagcaaag gtgcggtgat catgccgcca atcatcggtg cggcaatccg
     6181 gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac cggcgataat
     6241 caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc gcgccgtgat acaacgcctc
     6301 atccagcttc tgctcgctga atgtttgtgg attattcaac gacggctcgg cctctatggc
     6361 gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgaccccgg caagggcaat
     6421 aaagccggta cccgtcgcca cagaaagatg aaagcccatc caccacagta accagatgcc
     6481 gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca cccgacggaa
     6541 tgccagatac aacagcacga agatgatcat cagtgtcatc ggcaccatga gtttcagctt
     6601 atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg tgcccggttt
     6661 taactgcact ttttcagcta tcgctttttg caaatcgtga accaccgaca ccatgtcacg
     6721 atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt tcagcatcga
     6781 cggtccggta gagactttaa cgtcggccac gtctgccagg gtgatttgct gcttcatcgg
     6841 cgtcaggatc ggcaactggc gcagtgcctg cggactatcg cgccagcttt gcggataacg
     6901 cagattaatt ggataacggg caatcccttc caccgtttcg ccaaccatcg ccccgcccac
     6961 cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgtg cggctttttc
     7021 acggttaatc tcaacgttga tatagcgccc accttccagc cgctcggcaa gcgcagaagc
     7081 tacgcctggc accgttcgcg ccacttcttc aatttgctca gccatcgcgt cgatatccgc
     7141 cagcacagtg ccggaaactt taatgccgat ggggctttta atgccggttg agagcatatc
     7201 gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagac gcacggtgtt
     7261 atccagttcc tcaatgattt tgtccattgt catgcctggc cgccactgat cctgtggctt
     7321 aagctggatg gtcgtttcta ccatctccag cggagcagaa tcggtggcgg tttccgcttt
     7381 cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct tgtcggtttt
     7441 ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg gcatatacaa
     7501 caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga gcggccagag
     7561 aaccgtcagc accgaaagcg ccgccaccag cagcgtggtt ttcggccagt gcagtacttt
     7621 cagcaacagc ggatgataaa cacgaatcaa aaagcgattg agcggattac tgctttccgg
     7681 cggaattttg ccacggatcc agtaacccat caggataggg atcactacga tcgccagcag
     7741 cgccgcaccc gccatcgcat acgttttggt gaacgccaac gggccaaaca gacgcccttc
     7801 ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg ataatcagca gactgataaa
     7861 cagcgccggc cccacttcaa cagacgcatc ggtgatcacc tgccagcgcg ttttattatc
     7921 cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgcg cattctcgat
     7981 catgacgata gcggcatcga ccatcgcccc gacggcaatc gcaatgccac ccagcgacat
     8041 aatattggca ttcagcccct ggaagggcat gacaataaaa gcaatacaca accccagcgg
     8101 caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg cgcagactac
     8161 cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg cccggtcaat
     8221 gagctggctg cggtcgtatg ttgtaactat ctccacgcct tccggcagac tacttttcag
     8281 cgtttccagt ttgtccttca cggcggcgat cacttctcgg gcgtttttgc cggatcgcag
     8341 gatcaccacc ccgcccgcca cttcgccttc gccgtttaat gccccggcgc atctccgggc
     8401 caatctggtc cttcgcaaca tcgcgcagat aaacgggcac gccattttca ctggctttta
     8461 aaacgatgtg attaaagtcg tcgagcgttt gtagatagcc gctggcgcgc accatatatt
     8521 ctgcttccgc cagttcgatt gacgaaccgc ccgcttcctg gtttgaagca tccagcgcgc
     8581 tttttacttc ggcgagactg atgccatact gcgccaggcg ctgaggatcg ataaccacct
     8641 gatactcttt caccacaccg cctaccgacg ccacttccgc aacgtcagga atggttttca
     8701 gctcatattt gagaaaccag tcctgcaatg agcgtaaatc ggcaagatcg tgcttaccgc
     8761 tgcgatccac cagtgcatat tcatagatcc agccaacacc cgtggcatct ggtcccagct
     8821 cggcgctgac tcccgcaggc agcttaccct gtacctggtt gaggtactcc agcacccgcg
     8881 aacgcgccca gtacggatcg gtgccatctt cgaaaatgac atacacataa gagtcgccaa
     8941 actgcgagaa accacgcaca gtcttcgcgc caggcaccga caacatggtg gtggttagcg
     9001 gataagtcac ctgattttca acgatttgcg gtgcctggcc gggatagctg gttttaataa
     9061 tcacctgcac atcggagaga tccggcaacg catccactgg cgtattaatg atggtccagg
     9121 tgccccagat gctcagaaac aacgcgccca tcagcaccaa aaaacggttc gccaccgagc
     9181 gacgaataat ccattcaatc attggttatt ccctcaatgc gcatgggtag cactttcaga
     9241 gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa tcaatcagga acaggccgcc
     9301 ggaaaccacc ttttcacctt ccgccagacc agagcgtaat gcggtgacgc cttgcgatgc
     9361 ctggaaaaca gcaacgcgtt tcggtacaaa gcgcccgtcg gcatcaacgg taatcacccg
     9421 ctgttcgttg ccggtatcaa tcagcgcctc gtcggcgttg tcgacttcca gacgcagctg
     9481 cagcgtgcgg gtcgcggcat ccacgccagg tagcagcgtc catttgcgga tggtgagtgt
     9541 tttatccggt cgcgccggaa cggtgagggt aaactgcgag gcatctttca ccagccaggc
     9601 gatggactcc gggatcgcag cagtgaccca caccgggtcc ataccctgaa ttcgcgatta
     9661 cgcgcaatac ccctgagaac cgccatgaac aagggtttca tgatttgtga ttttagttta
     9721 gtacgcaatt tggtacacaa cactattttc acttcagcgg gtagtcatca aactcaccat
     9781 aacgagcatc gttgatgatg tatgtgatca ccacaaatat cacatctgga ctagttccgt
     9841 cttcatgcgc a
//
LOCUS       DADLZM010000142         9779 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLZM010000142 DADLZM010000000
VERSION     DADLZM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.143"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            83..364
                     /locus_tag="JNY73_003399"
     CDS             83..364
                     /locus_tag="JNY73_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049290.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="JNY73_003400"
     CDS             complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="JNY73_003400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY8049291.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="JNY73_003401"
     CDS             complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="JNY73_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY8049292.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3461..4831)
                     /gene="purB"
                     /locus_tag="JNY73_003402"
     CDS             complement(3461..4831)
                     /gene="purB"
                     /locus_tag="JNY73_003402"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY8049293.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="JNY73_003403"
     CDS             complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="JNY73_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY8049294.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="JNY73_003404"
     CDS             complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="JNY73_003404"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY8049295.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="JNY73_003405"
     CDS             complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="JNY73_003405"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY8049296.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="JNY73_003406"
     CDS             complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="JNY73_003406"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY8049297.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7968..9218
                     /gene="icd"
                     /locus_tag="JNY73_003407"
     CDS             7968..9218
                     /gene="icd"
                     /locus_tag="JNY73_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY8049298.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9321..9644)
                     /gene="iraM"
                     /locus_tag="JNY73_003408"
     CDS             complement(9321..9644)
                     /gene="iraM"
                     /locus_tag="JNY73_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY8049299.1"
                     /translation="MKWAVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
BASE COUNT         2450 a         2421 c         2438 g         2470 t
ORIGIN      
        1 catgaggatt ttgcttttga tataagcgac agccaccgcc agaaactgga agaatttagc
       61 ggggtgtaat gaggtcagca gcatgacaga agccgaaata acgggattaa tccgccgcgt
      121 tgccggaatc agccagcagg ctgacgaaca ggccacgcag ccggacagcg tgacagccga
      181 aaactatgcg cgtgttgttg ctgaggtgat gcgccgtgat ggtatagaac ttaatggcgt
      241 ggatatgcgc aacatacgaa ccagagttct tgagttgctg gcctaccgtc gccgcgtgga
      301 gatgtatcgg gagaaggaga aaatcacgta ccactggaag aagccggagc ggttacggcg
      361 gtaactcact gagattaaag caaagcgcaa aattgcgctg gctacatccc tcacatctga
      421 ggactgttaa ccagcaggcc ggaatatatt cagaaactac gaaattttcg cagtttaatt
      481 actcgtcagc ttaatcgcta acccgctctg atattttcgg aaacctcaat ttgaggaagt
      541 cggcgaggta actgtttgat attcccgata gcgcaaaaat gcgcggtcgg gcagaatgac
      601 caccacctca atcgtgacgg agagtgacaa ccagtgacac accgtgacaa aaagacggtt
      661 taaccgttca ttaaaaatcg acgtgatttt gtgctttccg gatgacgtgg ccaacgtcat
      721 tttttagcga aaaattctga ttaaggtcac tggctgaacg gtcataatga ccacgcagac
      781 catcgaggca accatagcga tcatcgttac ggttggagct tttccgccat tggtggaaaa
      841 ggtgcacatc tggatcatca ttacggtgat caagggtatg taaaacccac cagcctgatt
      901 aaaggttaac cggaaaaaaa atccggtagc cccgttatgg ggatccccat atcgacatta
      961 acgcccctca tgaattggtg cgcttccccc tggaaagatt acccgcctgt atatttcttg
     1021 tgtctatttg ttccacgttg tttcatacag tgcaccgaac ggtgtagtta atggtgtagt
     1081 caatttgcga tttttggcac tttttcaatg aaaattgttt aatcgaatca atgacttgat
     1141 gtagtggtaa aaggacatat ttattcatct ttcggcgcag aatgctggcg accaaaaatc
     1201 acctccatcc gcgcaccgcc cagcatgctc tctccggcga cgattttacc ctcatattgc
     1261 tcggtgattt cgcgggctac cgccagccct acaccttgcc cagggcgtaa agtatcaacc
     1321 cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg gccatcatcc
     1381 tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac aaactcgagg
     1441 caatatttac aggcattatc cagcacgttg cccatcacct cgacaaaatc gttctgctca
     1501 ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt gcgttgatac
     1561 actttgttca gcgctgaggt gagattgtcc agcagtgggg cgaccggatg cagctcgcgg
     1621 ctgagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat ttgctgtgaa
     1681 atgcggctga tttgctccag cattaccggc tcagcatcac tgacgctcat cttttcacta
     1741 cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact atgggtcagg
     1801 tcggtgagcg tcgtacggta tttgtcgtaa cgttcgcgtt cactttttaa caatcggttc
     1861 aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa ttcgcggtta
     1921 tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa actccaccag
     1981 gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac atagataaac
     2041 cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac caccacaatg
     2101 gttaattttg gcatccgcga tgttgccggg tagacgttta ccgccaccga gtgggtcatc
     2161 tctgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga atgatctcca
     2221 ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc attcgatttc
     2281 agccagtcag gctggatcat tttcatcagc cagggcacgt cacgttgcgc ccataaaagc
     2341 tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat attttcgggt
     2401 aactcgacat gcaatttatt gttttcccac tgcgcaaggg tatagaacag attgctctcg
     2461 ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat cagcgcgacc
     2521 attccgtagg caagcgaaag caccagtact accgctgccg ttgccaacag aaaacgtacc
     2581 cgcagcgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat tcgaacaggt
     2641 agccctggcc gcgaacggtg gtaatgactt cttgcggata ttgtgcctga atttttttgc
     2701 gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca tccggataga
     2761 gttgtagcat taacgaatct ttgctgacca ctttgccatt attgcgtatc aacgtttcca
     2821 taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat aattcacggc
     2881 gagagagatc aacctgaaac ggggggagcg aaatgacctg tgaagccaga ccgctattac
     2941 gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt ttagtcacgt
     3001 aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca cgggcggtta
     3061 ataccagaat cggcagtgaa acatcgttgc tacgccagcg gcgaatcagt gacagaccgt
     3121 cctcgtcagg caatccgaga tcgacaatcg caatatccgg taaatgttca ttgagataat
     3181 aatcggcttc tttggcatct tctgcgtcat cgacctgatg accagcatcc tgaatctgaa
     3241 ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc atttttattt
     3301 ctccctgtct taattattaa aatagtgtaa cgcgattatg tgtttatggg ggtaaacatt
     3361 aaataaacca gcggggaggg gaggtaaagt gaaaaaagaa aaagtggata atcttaataa
     3421 gcaggccgga cagcatcgcc atccggcact gatttgaggc ttatttcagc tcatcaacca
     3481 tcgtgatagc tcgaccaata tagttagccg gcgtcatcgc tttcaggcgg gctttctctt
     3541 cttctggcaa cgccagacca tcgataaact gcttcatgcc ttcggcgtca acgcgcttac
     3601 cgcgagtaag ctctttcagc ttctcgtacg gtttttcgat gccatagcga cgcataactg
     3661 tctggattgg ttctgccagc acttcccagt tgtgatccag ctcatccagc agatggtcac
     3721 ggttcacttc cagtttgctc acgcctttca gggtggattg atacgcaatc agcgcataac
     3781 cgatacccac gccgaggtta cgcagcacgg tggagtcggt caggtcacgc tgccagcggg
     3841 aaaccggcag tttgcttgcc agatgctgca ataccgcgtt ggaaaggccc agattccctt
     3901 cggagttttc gaagtcgatc gggttaactt tatgcggcat ggtggaagaa ccaatctcac
     3961 cagcaatggt tttctgtttg aagtggttaa gggcgatata accccagacg tcacggtcaa
     4021 agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa cagttcggca atgtagtcgt
     4081 gcggttcaat ctgggtagtg tacgggttcc attgaatacc cagcgaggtg acgaactctt
     4141 cgctgaactg atgccagtca acttccgggt aagcggcgat gtgggcgtta tagttaccga
     4201 ccgcgccgtt gattttgccg aggatctcca cctggttaag ctggcggtgc tggcgctcca
     4261 tacggtaggc gacgtttgcc atctctttac cgatggttga cggcgtggct ggctgaccgt
     4321 gggtacgaga cagcagcggg atatcgcggt actgaacggc gagatcttta atgccatcaa
     4381 tcagttgacg ccagtacggc aggatcactt catcacgcgc ggttttcagc attaatgcgt
     4441 gggagaggtt attgatatct tccgaagtac aggcaaagtg gatgaattca gaaaccgcgt
     4501 gcagttccgg gatctccgcc actttttctt tcaggaaata ctcaaccgct ttaacgtcgt
     4561 ggttagtggt acgctcgatg gttttgatgc gtgcggcatc ttcttcgctg aaattggcga
     4621 caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc aggaacttcc ttgatcgctg
     4681 cgtgcgcggc cagtttttgc agccaacgta cttcaacttg tacacggaat ttcagcaaac
     4741 catattcgct gaaaatcccg cgcagcgcgc tgagtttatc gccgtagcgt ccatcgacag
     4801 gggaaacggc ggtcagtgag gataattcca tagatcacaa ctccggggtt aaatgagcaa
     4861 gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa catcagttgt agacgtccgc
     4921 cgccgacctg gtgccagagc acggcggcgc gaatgcctgc cagcagggtt gcgcgaactt
     4981 tcgcctgcac ttgtgggctt tgcagtacag caggggaacc ggtgacctga atgcgcgggc
     5041 caagcgggct gatcacatca acatagatag cagccatcgc gctcatcagc gtttcggact
     5101 gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat ccggttgccc agagtgtcga
     5161 gcgcgccttt cgctgaggag agtttgcgct caagcaccat caagctgagt gtgtagcggg
     5221 ttaattcggc gtttaagccc tggcaactgc tggcattgag cacgccgagc agggtttcca
     5281 gcccgacgcg caggttggct tcgctaccgc caaaaaccgc cagcgtcgag ctggggttca
     5341 tatcaataat actgttgagt gagacgtgta gcgcatcggc atcacaatgc ccctggtgag
     5401 cgagttgttg caccaggcgt gccgactgac aaataccggc cagggcgagg gtgatgtcat
     5461 agtaattctt tgccacgttc actgcttcct tgttttaagt aaagataata atcagaccgg
     5521 cagcggcaga cgctgctcaa taataccgcc accgaggcac acttcaccgt tatagaagac
     5581 ggcagactgg cccggcgtca cggcggcaac cggctcatcg aaaatcactt taatgcgatc
     5641 gtcgtccagc gccttgacgg tgcacggaat gtcggtctgg cgatagcggg tttttaccgt
     5701 gcaacgcata gtgccggtga atggttcgcg atcgacccag tgcagctgct gggcaatcaa
     5761 cccgacagac atcagccgcg ggtgttcatg gccctgagcg acaaccagaa tgttgttttc
     5821 gacgtctttg tccaccacat accacggttc ttcggtgcct tctttggtgc caccgatacc
     5881 cagaccttta cgctgaccga gagtgtgata catcagcccc tggtgctcac caatttcatc
     5941 gccatcgacg gtaatgattt tgcccggttg cgccgggaga taacggccca ggaactcgcg
     6001 gaatttacgt tcgccaatga agcaaatgcc ggtagagtct tttttcttcg cggtgaccag
     6061 accaagatct tcagcaatct tacgcacctg cggtttttcc agttcgccga ccgggaacag
     6121 gctttgcgca atctgctcat ggctgagcgt ataaaggaag tagctctggt ctttattgct
     6181 gtccagacca cgcagcaggc ggctcttgcc atcgacatcg gcccgacgta cgtaatgacc
     6241 ggtagcgata taatcggcac ctaaatcttc ggcggcaaat tcgaggaagg ctttaaattt
     6301 gatctccttg ttgcacagaa tatccggatt cggcgtgcga ccggctttat attcggcaag
     6361 gaacagttcg aagacgttgt cccagtactc ggcagcaaag ttaacggtgt gcagttcaat
     6421 gccgagcttg tcgcagaccg cctgggcatc agccagatcc gctgccgctg tgcaatattc
     6481 ctcaccgtcg tcttcttccc agttcttcat aaacaggcct tcgacctgat atccctgttg
     6541 ttgcaacagc caagcagaaa cggaggaatc gacaccgccg gacatgccga cgattacttt
     6601 ttttgcggtt tcagacattg gatcactcac gacattgaac ttcaaggcgg cgtattctat
     6661 cacgcagcct tttagatgac accctttgta aaaggccagt taaaatcgcc aatcatctcc
     6721 agcggataac gttgcccgct ttgataacaa cgaatacttt ccgccaccag cggcgaacga
     6781 agatttgacg cctgtaaaat ttcttcggcg ctgacccaac ggcagcagtc gatatcgctg
     6841 tcatgaggct gggtcgggca tatttgctca agctcaatgg caaagaggaa acgcaaaaac
     6901 ggcgttttat ctggcgcaat ccactgatgc atacgaataa agtgttgcgg ctgcgcgctg
     6961 atgccggttt cttcccacag ttcacgggcg gcggcttcca ctaaggtttc atcggcttcc
     7021 agatgcccgg caggttggtt ccataacgct ttaccattaa tcgtctcttc aacgactaaa
     7081 aatttgcctt ctgcgtgcac cacgcaagca acggtaacgt gcggtttaaa cattttgcat
     7141 cctcaatctg tcacttctcg ccattcgcca ttggcaagat tatccaaaga gtaatctccc
     7201 atcgcatagc gaatcagtcg cagcgtgggg aaaccaacat gggcggtcat gcggcgcacc
     7261 tggcgattac gtccttcata taaggtgatc ttcagccagc tggtgggaat acttttgcgt
     7321 tcacgaattg gtggattccg cggccataac cacgcgggtt cgtcaaccag ctccgcgcct
     7381 gcgggcaggg tagggccatc atttaaggtt acgccattgc gcaaggcttc aagtgcgtct
     7441 tgtgtgggaa taccttccac ctgcacataa tagatttttc cggtgcgttt acccggctgg
     7501 gttaaacgcg cctgcaacgc gccgttattg gtcagcacca gcaacccttc gctatcgcgg
     7561 tcaaggcgac ctgctgcata aacgccctga accgggatga attcttttaa tgttttgcgt
     7621 ccggcttcat cggtgaactg cggaagaaca tcgtagggtt tattgaagag gatcacacgc
     7681 gtgggctggt tttcatgttt acgcctggta gaacgttgcg agctgaatcg cttaacctgg
     7741 tgatttctaa aagaagtttt ttgcatggta ttttcagaga ttatgaattg ccgcattata
     7801 gcctaataac gcgcatcttt catgacggca aacaataggg tagtattgac aagtcaatta
     7861 caaatcatta acaaaaaatt gctctaaagc atccgtatcg caggacgcaa acgcatatgc
     7921 aacgtggtgg cagacgagca aaccagtagc gctcgaagga gaggtgaatg gaaagtaaag
     7981 tagttgttcc ggcacaaggc aagaagatca ccctgcaaaa cggcaaactc aacgttcctg
     8041 aaaatccgat tatcccttac attgaaggtg atggaatcgg tgtagatgta accccagcca
     8101 tgctgaaagt ggtcgacgct gcagtcgaga aagcctataa aggcgagcgt aaaatctcct
     8161 ggatggaaat ttacaccggt gaaaaatcca cacaggttta tggtcaggat gtctggctgc
     8221 ctgctgaaac ccttgatctg attcgtgaat atcgcgttgc cattaaaggc ccgctgacca
     8281 ctccggttgg tggcggtatt cgttctctca acgttgccct gcgtcaggaa ctggatctct
     8341 acatctgcct gcgtccggta cgttactatc agggcactcc aagcccggtt aaacaccctg
     8401 aactgaccga tatggttatc ttccgtgaaa actcggaaga catttatgcg ggtatcgaat
     8461 ggaaagctga ctctgccgac gccgagaaag tgattaaatt cctgcgtgaa gagatgggcg
     8521 tgaagaaaat tcgcttcccg gaacattgcg gtatcggtat taagccgtgt tctgaagaag
     8581 gcaccaaacg tctggttcgt gcagcgatcg aatacgcaat tgctaacgat cgtgactctg
     8641 tgaccctggt gcacaaaggc aacatcatga agttcaccga aggcgcgttt aaagactggg
     8701 gctaccagct ggcgcgtgaa gaatttggcg gtgaactgat cgacggcggc ccgtggctga
     8761 aagttaaaaa cccgaatacc ggcaaagaga tcgtcataaa agacgtgatt gctgatgcat
     8821 tcctgcaaca gatcctgctg cgtccggctg aatatgatgt tatcgcctgt atgaacctga
     8881 acggtgacta catttctgac gctctggcag cgcaggttgg cggtatcggt atcgcccctg
     8941 gagcaaacat cggtgacgaa tgcgccctgt ttgaagccac ccacggtact gcgccgaaat
     9001 acgccggtca ggacaaagta aaccctggct ctattattct ctccgctgag atgatgttac
     9061 gccatatggg ctggactgaa gccgcagacc tgattgttaa aggtatggaa ggcgcgatca
     9121 acgccaagac cgtaacctat gacttcgagc gtctgatgga aggcgctaaa ctgctgaaat
     9181 gttcagagtt tggtgatgcg atcatcaaga atatgtaatc actacatgtg ttaaatattt
     9241 aaacgggcgt ataacacgcc cgttgtttta tttatgtgga tattattaat agcatatcga
     9301 gcatatttat ataaaaccta ttacttgagc ccatatgggc atatttttat aatacaactc
     9361 ttatgcaaac atttatttgt tatttcgctt tctcctggag gacactcttg actgcttttg
     9421 agtaaactcc ataaattctt gttgaatggt gcaatgttat aaatagtaat aggatattct
     9481 ttttccttaa ggataatacc aagcttaacc ggtgtaaata tccttccagg aggaagaaat
     9541 atagtagatt gataccagat gatcattttc atatcacccc atatggctga aaaagatatg
     9601 ccgcatgtag gttgaattac cgtgtcaatt actgcccact tcatttgtta tgtcttatcc
     9661 cacggtattt aatatagttc atttggatgt tcatttcttt attttgcata tgagtatatt
     9721 accccttcaa aaaataaatt aattaaaacg attgcttata taaaacaaaa tttaaatca
//
LOCUS       DADLZM010000143         9540 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLZM010000143 DADLZM010000000
VERSION     DADLZM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.144"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..305
                     /locus_tag="JNY73_003409"
     CDS             <1..305
                     /locus_tag="JNY73_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8049300.1"
                     /translation="CTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFT
                     LGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            461..1996
                     /gene="gadC"
                     /locus_tag="JNY73_003410"
     CDS             461..1996
                     /gene="gadC"
                     /locus_tag="JNY73_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY8049301.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2127..3446
                     /locus_tag="JNY73_003411"
     CDS             2127..3446
                     /locus_tag="JNY73_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY8049302.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3811..4956
                     /locus_tag="JNY73_003412"
     CDS             3811..4956
                     /locus_tag="JNY73_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8049303.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4881..5498
                     /locus_tag="JNY73_003413"
                     /pseudo
     CDS             4881..5498
                     /locus_tag="JNY73_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5756..6088
                     /gene="ddpX"
                     /locus_tag="JNY73_003414"
                     /pseudo
     CDS             5756..6088
                     /gene="ddpX"
                     /locus_tag="JNY73_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6102..6998
                     /locus_tag="JNY73_003415"
     CDS             6102..6998
                     /locus_tag="JNY73_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY8049304.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6995..7981
                     /locus_tag="JNY73_003416"
     CDS             6995..7981
                     /locus_tag="JNY73_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8049305.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7974..8900
                     /locus_tag="JNY73_003417"
                     /pseudo
     CDS             7974..8900
                     /locus_tag="JNY73_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8956..9387)
                     /gene="osmC"
                     /locus_tag="JNY73_003418"
     CDS             complement(8956..9387)
                     /gene="osmC"
                     /locus_tag="JNY73_003418"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY8049306.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2322 a         2339 c         2402 g         2477 t
ORIGIN      
        1 tctgtaccgg tcgcccggac gaaggcatcc cggcggtttg cttcaaactg aaagatggtg
       61 aagatccggg atacaccctg tacgacctct ctgaacgtct gcgtctgcgc ggctggcagg
      121 ttccggcctt cactctcggc ggtgaagcca ccgacatcgt ggtgatgcgc attatgtgtc
      181 gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa gcctccctga
      241 aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc tttaaacata
      301 cctgataacg tttaacggta acggtgtccc gaaacgaacc cgtttcggga taatttccaa
      361 agtctgttca ctggcattag caacggaaaa tattgttctg aatacgcttc agaacaaaac
      421 aggtgcggtt ccgacaggaa taccgtttta gggggataat atggctacat cagtacagac
      481 aggtaaagct aagcagctca cattactcgg attctttgcc ataacggcat cgatggtaat
      541 ggctgtttat gaatacccta ccttcgcaac atcgggcttt tcattagtct tcttcctgct
      601 attaggcggg attttatggt ttattcccgt gggactttgt gctgcggaaa tggccaccgt
      661 cgacggctgg gaagaaggtg gtgtcttcgc ctgggtatca aatactctgg gtccgagatg
      721 gggatttgca gcgatctcat ttggctatct gcaaatcgcc attggtttta ttccgatgct
      781 ctatttcgtg ttaggggcac tctcctacat cctgaaatgg ccagcgctga atgaagaccc
      841 cattaccaaa actattgcag cactcatcat tctttgggcg ctggcattaa cgcagtttgg
      901 tggcacgaaa tacacggcgc gaattgctaa agttggcttc ttcgccggta tcctgttacc
      961 tgcatttatt ttgatcgcat tagcggctat ttacctgcac tccggtgccc ccgttgctat
     1021 cgaaatggat tcgaagacct tcttccctga cttctctaaa gtgggcacac tggttgtatt
     1081 tgttgccttc attttgagtt atatgggcgt agaagcttcc gcaactcacg tcaatgaaat
     1141 gagcaacccc gggcgcgact atccactggc tatgttactg ctgatggtgg cggcaatctg
     1201 cttaagctct gttggcggtt tgtctattgc gatggtcatt ccgggtaatg aaatcaacct
     1261 ctccgcaggg gtaatgcaaa cctttaccgt tctgatgtcc catgtggcac cggaaattga
     1321 gtggacggtt cgcgtgatct ccgcactgct gttgctgggt gttctggcgg aaatcgcctc
     1381 ctggattgtt ggtccttctc gcgggatgta tgtcacagcg cagaaaaacc tgctgccagc
     1441 ggcattcgct aaaatgaaca aaaatggcgt accggtaacg ctggtcattt cgcagctggt
     1501 gattacttct atcgcgttga tcatcctcac caataccggt ggcggtaaca acatgtcctt
     1561 cctgatcgca ctggcgctga cggtggtgat ttatctgtgt gcttatttca tgctgtttat
     1621 tggctacatt gtgttggttc ttaaacatcc tgacttaaaa cgcacattta atatccctgg
     1681 tggtaaaggg gtgaaactgg tcgtggcaat tgtcggtctg ctgacttcaa ttatggcgtt
     1741 tattgtttcc ttcctgccgc cggataacat ccagggtgat tctaccgata tgtatgttga
     1801 attactggtt gttagtttcc tggtggtact tgccctgccc tttattctct atgctgttca
     1861 tgatcgtaaa ggcaaagcga ataccggcgt cactctggag ccaatcaaca gtcagaacgc
     1921 accaaaaggt cacttcttcc tgcacccgcg tgcacgttca ccacactata ttgtgatgaa
     1981 tgacaagaaa cactaaccat gtctgaatgg ttataagaca agggagcgat aattcatcgc
     2041 tccctttttc gtgcttgctg tcgtattgac ttaaccggga taagtacgag aatgagcgca
     2101 catctgttta ccggaaacca gcacatatgg atatctgctc ccgaaacaag aaattaacga
     2161 ttagaagacc agcgatactg gttgcattgg cacttttact gtgtagttgt aaaagcacgc
     2221 ctccagagtc catggtgaca ccaccagcag gttcaaagcc accagccacg acgcaacaat
     2281 cgtcacaacc gatgcgtggc atctggctgg ccacggtttc tcgcctcgac tggccaccgg
     2341 tttcctcggt taacattagt aatcccacca gccgagcccg tgtacaacaa caggcgatga
     2401 tcgacaaact ggatcatctc caacgtctcg gcataaacac ggtctttttc caggtcaagc
     2461 cggacggtac cgccctgtgg ccatcgaaaa ttttgccgtg gtccgatctt atgaccggta
     2521 agattggtga aaatccgggt tacgatccgc tgcaattcat gctcgacgaa gcccacaagc
     2581 gtgggatgaa agtacacgcc tggtttaacc cctatcgcgt atcggttaat acgaagcccg
     2641 gtactatcag ggaactgaat agcactctgt ctcaacaacc ggcgagcgtc tatgtgcaac
     2701 accgtgactg gatcagaacg tctggcgatc gctttgtcct cgacccgggc atccctgagg
     2761 ttcaggactg gatcacatca atagtcgctg aagtggtttc ccgctatccg gtagatggcg
     2821 tgcagtttga cgactatttc tatactgaat caccgggttc acggctaaat gataacgaaa
     2881 cgtaccgtaa atacggaggc gcatttgcgt caaaagcaga ctggcggcgc aacaatactc
     2941 agcagttaat tgcaaaggta tcacacacca ttaaaagcat taagccggga gtcgaatttg
     3001 gcgttagccc ggcaggcgtg tggcgtaacc gatcacacga tccgctcggt tccgataccc
     3061 gaggcgcggc agcctatgac gaatcctacg ctgacacccg tcgatgggtg gaacaaggat
     3121 tgctggatta cattgctccc caaatttact ggccgttctc acggagtgcc gcgcgttatg
     3181 acgtgttggc aaaatggtgg gcggatgtcg ttaaaccgac caggacccgc ctgtatatcg
     3241 gtatcgcctt ctataaagtg ggtgaacctt caaagataga gccagactgg atgattaacg
     3301 gcggcgtacc ggaactgaaa aagcagctcg atcttaacga tgcggtacca gaaattagcg
     3361 gcaccatctt gttccgtgag gactatctga ataaaccgca gactcaacaa gcggtcagct
     3421 atctgcaaag tcgttggggc agttaaacta ccatgcttac tttgtaacaa gccggtgctt
     3481 tacccaccgg cttgttacac cttgtgaaat atcccactag taatcatgct tacataagtc
     3541 aaattaacca cacttagtta aacagctata aatccctaat aaaaatgtat gaatatgaaa
     3601 aaccatacga tattcgctgt caccgaaaaa taattacgat gggctatact cttctgaaga
     3661 acagtagcgt agattgaaac tgtcttaagt cgcttatttc ttttcagtat atcttcatat
     3721 ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg tgtttcgttt tgattccgtc
     3781 tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag aatgaaagat
     3841 gagtggaccg gacttgtcga acaggcagat cctctcattc gtgctaaagc cgcagaaatt
     3901 gcccttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg catcgacccg
     3961 catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag tgcgatggaa
     4021 cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct aatacaaatc
     4081 cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat tgtcgagatg
     4141 gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga ttattccgcc
     4201 gcagaaaaac ttcaggtcta ccatttctcg attaacagta ttgatatcgc gatggaagtg
     4261 atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga aaactatcgt
     4321 atcttctcgt tactggaaaa cgccgaagaa gaaaaagaac ggcaaatagc ctcaatactt
     4381 tcatgggaaa tagatattat ctataaagtc ctgctggatt ctgatttagg cagcagtttg
     4441 cctttaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca ttattttagc
     4501 ggcattgccg aagtgggcca tatctcccgt ctgattcagg atttcgacgg tattttcaat
     4561 caaaccatgc gtaacaccag gattttgaat aacagaagtt tgcgggtgaa atttctatta
     4621 cagataagaa ataccgtatc gcagattatt accttgctgc gtgaattgtt tgaagaagta
     4681 tcgcgccacg aagtcggtat ggatgtactg acgaaattac ttaaccgccg tttcctgccg
     4741 actatcttca aacgcgaaat tgcccatgcc aaccggaccg gtacaccgct gtcagtgctg
     4801 attattgacg ttgataaatt caaagagatc aacgatacgt ggggccataa cactggcgat
     4861 gaaattctgc gtaaagtctc attcctctcg cagaagagat tggtgaaatc gaaaatattg
     4921 ggcgctgggt catcgcggaa gcttgccgtc agttagcaga atggcgtagc cagaatattc
     4981 atatcccggc gttatccgtg aacttgtcgg cgctgcactt tcgcagtaat cagctgccta
     5041 atcaggtgtc tgatgcaatg caagcctggg gtattgacgg ccaccagctg acggtggaaa
     5101 tcacggaaag catgatgatg gaacacgata ccgaaatctt taagcgcatt cagatcctgc
     5161 gtgatatggg cgtgggttta tcggtagatg attttggcac gggcttttcc ggattatccc
     5221 gcttagtcag tcttccggta acggaaatca aaattgacaa aagctttgtc gatcgttgtc
     5281 tgacggaaaa acgcatcctg gccttacttg aagccattac cagcattggg caaagcctca
     5341 atttaaccgt cgtggcggaa ggggtcgaaa ccaaagagca atttgagatg ctacgtaaga
     5401 tccactgtcg cgttattcag ggatatttct tttcccgccc ccttcccgcc gaagaaattc
     5461 caggctggat gagcagcgtg ttaccgctga aaatctgaca aattcctctc gcccgcactc
     5521 gcgggttttc ctttaaggtg acactgtcac ttaaatactg tgatttcagc tacgattccg
     5581 ggagattcct tccttaactt tccctcttgc accaaaactg cactacgctg cacaaatgtt
     5641 gatcaaaagt taaaaaatat aactttaagc cagcattctg gcgcttatcc cggcatggca
     5701 tgagatctgc ataagcggaa agcgcagcaa tttttgtctt atacaggaac cgtttatgtc
     5761 ggataccacc gaactggttg atttagccgt gatcttccct gatctggaga tcgaattgaa
     5821 atatgcctgc gttgataaca tcacaggtaa agctatttat cagcaagcgc gttgtctgct
     5881 acataaagat gcgattaccg cgctggcgaa aagtatcagt atcgcccagc tgtcaggctt
     5941 acaactggtg atttacgatg cgtatcgccc acaacaagca caggcgatgt tgtggcaagc
     6001 ctgcccagac ccgcaatatg ttgttgatgt gacggtcggt tctaatcaca gccgtggcac
     6061 ggcgctcgac ctgacgcttc gtgatgacgt ggaggtggtg aatgatgcta agcgaggaaa
     6121 cgtccgccgt acgcccacaa aaacaaacgc gatttaacgg tgcaaaactg gtatggatgc
     6181 tgaaaggcag tccgctcacc gtgaccggcg cagtcatcat tgtattaatg cgactgatga
     6241 tgattttttc accgtggctg gcgacgcatg atcccaacgc cattgattta accgcccgcc
     6301 ttttgccgcc ttctgcggcg cactggtttg gcaccgatga agtgggacgc gatctgttta
     6361 gccgcgtact ggtcggcagt cagcaatcaa ttctcgccgg attagtggtg gtcgccattg
     6421 cgggtatgat tggttcgcta ctcggatgtc tatccggtgt gcttggcgga cgcgcagacg
     6481 ccatcatcat gcgcatcatg gacattatgc tgtcgattcc ttcgttggta ctgacaatgg
     6541 cactggctgc cgctctcggg ccgagtttat ttaacgccat gctggcgatt gctattgtgc
     6601 gaattccctt ttatgtgcgc ctggcgcggg ggcaagcatt agttgtacgc cagtatacct
     6661 atgttcaggc ggcgaaaacc tttggcgcgt ctcgttggca tctgatcaac tggcatattt
     6721 tacgtaactc cctaccgccg ctgatcgtgc aggcatcgct ggatatcggt agcgcgattt
     6781 taatggccgc cacgttggga tttattggcc tgggtgctca acaaccgagt gctgaatggg
     6841 gcgcgatggt agcgaatggt cgcaactatg tactcgatca gtggtggtat tgcgcatttc
     6901 cgggggcagc gattttgctt accgccgtcg ggtttaatct ctttggtgat ggtattcgcg
     6961 atctgcttga cccgaaagca ggaggaaagc agtcatgacc caacccgttc tggacattca
     7021 acagctgcat ttgagtttcc ccggttttaa cggtgatgtt cacgcgctca acaatgtgtc
     7081 cttgcagatt aaccgcggcg aaattgtcgg tctggtggga gaatccggct caggtaaatc
     7141 agtcaccgca atgctgatta tgcgtctgct accgacgggc agttattgcg tacatcgggg
     7201 gcagatttca ctgctgggag aagatgttct taacgctcgg gaaaaacagc ttcgtcagtg
     7261 gcgcggcgca cgagtggcga tgatctttca ggaaccgatg accgccctta atccgacacg
     7321 tcgaataggt cttcagatga tggacgtaat ccgccatcat caaccaataa gtcgtcggga
     7381 agccagagct aaagcgattg ccctgctgga agagatgcaa atcccggatg ccgtggaagt
     7441 tatgtcgcgc tatccgtttg agctttcagg tggtatgcgc cagcgggtaa tgattgcgct
     7501 ggcattctcc tgcgagccgc aattgattat tgccgacgaa ccgacgacgg cgctggacgt
     7561 cacagtacag ttgcaggtac tgcgtttgct taaacataaa gcacgcgcca gtggaactgc
     7621 ggtactgttc atcagccatg atatggccgt ggtgtcgcaa ctgtgcgata gcgtttacgt
     7681 gatgtatgcc ggaagcgtaa ttgaaagcgg cgggacggca gacgttatcc atcatccccg
     7741 gcatccgtat accattggtt tgctgcaatg cgcaccagaa catggagtac cacgccagcc
     7801 attacccgcc attccaggga cggtaccaaa cctcacccat ttgcctgacg gctgcgcttt
     7861 tcgcgatcgt tgctatgcgg caggtgcaca gtgtgaaaac gtcccggcgc tgacagcgtg
     7921 tggtgacaac aaccagcact gcgcctgttg gtatccgcag caggaggtca taagtgtctg
     7981 acacgttatt aacgttacgc gacgtccata tcaattttcc ggcccgtaaa aactggcttg
     8041 gtaaatctac ggaacatgtt catgccatta atggtattga tttacagatc cgccgtggtg
     8101 aaaccttagg gatcgtcggc gagtcaggct gcggcaaaag caccctcgca cagcttttaa
     8161 tgggcatgct gcaaccgagc cacgggcagt acatccgttc aggctcacaa cgcattatgc
     8221 agatggtgtt tcaggacccg ctctcttcgc ttaatccgcg cttaccggtg tggcgcatca
     8281 tcacagaatc gctctggata gctaagcgta gtagtgaaca acagcggcga gcgttggcag
     8341 aggagttggc tgtgcaggtg ggtattcgtc cggagtatct cgaccgcctg cctcatgcgt
     8401 tctccggcgg gcagcggcaa cgcatcgcca ttgccagagc actctcttcg cagcctgacg
     8461 tgattgtgct tgatgagcca acctcggcgc tggatatctc cgtgcaggcg cagatcctca
     8521 atttactggt aacgctacag gaaaatcacg ggctgactta tgtgctgatt tcacacaatg
     8581 tctcggtgat acgtcatatg agcgatcggg tggcggtgat gtatctcggg cagattgtag
     8641 aactgggcga cgcgcaacag gtgttgactg cttcggcaca tccatacacc cgattattgc
     8701 tggattccct ccccgccatt gataaaccgc tggaggaaga atgagcatta cgtaaaacgg
     8761 agctgccggg aaaccgcacg ttgccgcaag gctgtttttt ctacgaacgt tgccctctgg
     8821 caacccacgg atgtgaagtc cggcaatcat tagcaataag ggaggacgga cgtgagctcc
     8881 ggtgctggcg ggcgctgtag agatgctaac aacgcatcag gcattcacgc gcctgacgcg
     8941 tcatccggca atgctttacg atttcaactg gtaatccagc gtaatttccg ctttcagtac
     9001 ctgagagacc gggcatcctg cttttgcttt ctggatgatg ccgtcaaagg tagaggcatc
     9061 aatacccggc accgcaactt cactcttcag tgcgattttc gtaatcgcaa aaccggcatc
     9121 cactttatcc agcgacacat cggcggtggt atcaattgat gttggcgtga atcccgcttc
     9181 ccccagcatt aatgaaagcg ccattgagaa acatgcggca tgcgctgcgc caatcagttc
     9241 ttcagggttg gttccttttt cgccttcaaa acgcgtgtta aatccatacg gctgttggtt
     9301 cagcacgccg ctctcggtgg atactgttcc cttcccgcgt ttgatatcgc cttcccagtg
     9361 tgcctgacct ttcttatgga ttgtcattgt tgctctcctg tgggcttaaa aaggaaatta
     9421 tagactacgt gagaaacagc acgaggataa gcaggatatt ccgaataaaa tacggcgaaa
     9481 atattacttc tgcacttaaa ttaaattccc acaatagata accgggaata tatatctcaa
//
LOCUS       DADLZM010000144         9525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLZM010000144 DADLZM010000000
VERSION     DADLZM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.145"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(435..641)
                     /locus_tag="JNY73_003419"
     CDS             complement(435..641)
                     /locus_tag="JNY73_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY8049307.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JNY73_003420"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JNY73_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY8049308.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="JNY73_003421"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="JNY73_003421"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY8049309.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="JNY73_003422"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="JNY73_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /locus_tag="JNY73_003423"
     CDS             4795..5367
                     /locus_tag="JNY73_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY8049310.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8042
                     /gene="kdbD"
                     /locus_tag="JNY73_003424"
                     /pseudo
     CDS             5360..8042
                     /gene="kdbD"
                     /locus_tag="JNY73_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8039..8716
                     /gene="kdpE"
                     /locus_tag="JNY73_003425"
     CDS             8039..8716
                     /gene="kdpE"
                     /locus_tag="JNY73_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY8049311.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8991..9095
                     /locus_tag="JNY73_003426"
     CDS             8991..9095
                     /locus_tag="JNY73_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY8049312.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9406..>9525
                     /locus_tag="JNY73_003427"
     CDS             9406..>9525
                     /locus_tag="JNY73_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY8049313.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2116 a         2396 c         2640 g         2373 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa acatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctccccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttaccac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcta ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttaatcgca ctgtttttta ttcaacaagg
     1621 tgcgctgcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatactt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgaccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttaccg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgccgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctccgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gcgaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gctgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctatt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg actagccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctggcg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaac cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcagaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggtggcgct attttatgga cgctggcctt cggtggttgc caccgtcatt
     6721 aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat
     6781 gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt
     6841 actgctggcg tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac
     6901 ttatatgaaa tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc
     6961 agcgaacaat ttattgcctc tacgtttcat gcccgcagtc aggtgttgtt gcccgatgac
     7021 aacggtaaat tgcagccgtt aacacatccg caaggaatga cgccatggga cgatgccatc
     7081 gcgcagtgga gttttgataa aggcctgcct gcgggcgcgg gcaccgacac gttacccggt
     7141 gtaccgtacc agattttgcc gctaaaaagc ggcgagaaaa cctacggctg gtggtggtgg
     7201 agccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg gagactttta
     7261 cgctgttagt cgccaatgcc cttgagcggc tgacgctaac cgccagcgaa gaacaggcgc
     7321 ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcagcgctt tcgcatgatt
     7381 tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa
     7441 gcgaaggatc accccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta
     7501 cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga
     7561 agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ctggaaccgg
     7621 gtttatcgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg
     7681 ggccactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg
     7741 cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct
     7801 gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc
     7861 gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg gttggcaatt tgtcgggcga
     7921 ttgtggatgt acacggcggc accattaccg cgttcaaccg accggaaggt ggtgcctgtt
     7981 ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt
     8041 gacaaacgtt ctgattgttg aagatgaaca ggctattcgt agctttctgc gcacggcgct
     8101 ggagggcgac gggatgcgcg tctttgaggc cgaaactctg caacgcggct tgctggaagc
     8161 ggcaacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat
     8221 tgagtttatc cgcgacctgc gccagtggag cccggtgccg gtgattgtgc tttccgcacg
     8281 cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt atctgagtaa
     8341 gccgtttggc attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc
     8401 caccgccgcg cccgatccgc tggtgaaatt ttccgatgtt accgtcgatt tagccgcccg
     8461 cgtgattcac cggggtgagg aagaggtgca tctcacacca attgagttcc gcctgctagc
     8521 ggtgctgctc aacaatgccg gaaaagtact cacccagcgc cagctcctta accaggtgtg
     8581 ggggccaaac gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca
     8641 aaaactggaa caggatccgg cccggcctcg ccacttcatt actgaaaccg gtattgggta
     8701 tcggtttatg ctttgaattg ttattcaata aattcgctta aatagtgatt aatttattat
     8761 taaacctgcg ccgaatatta ttatttggca ggtttaattt cttaacgcaa atataaaaca
     8821 cgaaattaca acatttaagt aacaaaacag catctaatat ggcgatattc ataattaatg
     8881 atttattatc tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc
     8941 atggatagaa taactctacc ttcaactgac acagcaagcg gtaaaggtaa atggaaaata
     9001 ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt gcccatcgta
     9061 atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac
     9121 gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata
     9181 tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat
     9241 gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac
     9301 tgatttattt atcagcggtg gagctttgct ttttttccgg cgtgatcgat ttctcctttg
     9361 agaaattgag gacctgctat tacctgaaat aaagagatga ataaaatgtc agaattaaaa
     9421 attgccgtta gccgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatatt
     9481 gacgaaagta attatattga cgttgccgcc attattttat cagtc
//
LOCUS       DADLZM010000145         9519 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLZM010000145 DADLZM010000000
VERSION     DADLZM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.146"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(194..1447)
                     /locus_tag="JNY73_003428"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="JNY73_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="JNY73_003429"
     CDS             1554..2447
                     /locus_tag="JNY73_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8049314.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="JNY73_003430"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="JNY73_003430"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY8049315.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="JNY73_003431"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="JNY73_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY8049316.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="JNY73_003432"
     CDS             complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="JNY73_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY8049317.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYKVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVG
                     LLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTL
                     FIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEY
                     QLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="JNY73_003433"
     CDS             complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="JNY73_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY8049318.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6671..7525)
                     /locus_tag="JNY73_003434"
     CDS             complement(6671..7525)
                     /locus_tag="JNY73_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY8049319.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="JNY73_003435"
     CDS             complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="JNY73_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY8049320.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDNPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8155..9240)
                     /locus_tag="JNY73_003436"
     CDS             complement(8155..9240)
                     /locus_tag="JNY73_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8049321.1"
                     /translation="MTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQ
                     DDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSA
                     KFERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKT
                     TGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIH
                     PIPAYTSGFDGWDAPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDA
                     QARGIRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSI
                     NILTSHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2473 a         2412 c         2460 g         2174 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 ccagcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 tcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcgaaaa ggccacgaaa ctggggtgga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gcgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagctc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actttgtaga gtaacgcgtc gctatgatta
     5221 atcagattgc taaccagcaa tgccacgacg gcggaaataa tcaccgggcc gagagaggcc
     5281 aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga
     5341 taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca ttcctggcgt
     5401 ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct
     5461 tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg
     5521 cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca
     5581 tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg
     5641 ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga
     5701 ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg acgaaacccg
     5761 gcaacggcaa agacagcgag aataccgacg actgttgcga taagcagacg gcggaacata
     5821 gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg
     5881 ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt taagagataa
     5941 aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg taatcgtcac catccggcaa
     6001 tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac agttgtgatt
     6061 gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg
     6121 ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca
     6181 attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg
     6241 aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca
     6301 tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga
     6361 ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct
     6421 gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt
     6481 ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg
     6541 cttccgcact ctctggagcg taataaaaca gcgctcccaa cacgcgcgcc gcgacagaaa
     6601 aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg
     6661 caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca taaaagagca
     6721 cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt ccggcaacgt
     6781 gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg cataacaata
     6841 ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg
     6901 atgagtgaat agacgccaga gacaaacctt gcagcacaat cacctcggca caagcgatca
     6961 gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg gctgcccgca
     7021 gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag
     7081 tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga
     7141 cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca
     7201 ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg
     7261 cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca
     7321 gtggcgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga
     7381 aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt tttggcggcg aaccagccaa
     7441 ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg
     7501 gccattcatg ccatccattt cccattttac acttcctccg gattagccag ataaccagtg
     7561 gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa
     7621 tgcgcgcgcg gcagtggcgc gacggcagcc agagtaccgt gcttctggcg cagctcttca
     7681 attggaccga actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc
     7741 tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc agcattgtac
     7801 tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca
     7861 tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta
     7921 catgcggggt tatcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg
     7981 tgccagatgc cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa
     8041 ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
     8101 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
     8161 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgctagcg gtgaagggcg
     8221 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
     8281 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
     8341 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
     8401 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
     8461 cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc ccgttaactg
     8521 cagtggatag gttttccgca ggggagcgtc ccagccatca aaacctgagg tatacgcagg
     8581 aatgggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct tcgccagtcg
     8641 ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct gtggatcttc
     8701 acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt
     8761 cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag
     8821 atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc
     8881 taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga
     8941 ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat gagagataag
     9001 gtcttcttgc tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc
     9061 cgtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc
     9121 atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa
     9181 aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat
     9241 tttcgtacca tggtcgatgg catctgtcca tgtaaacatc tggggtcctt acagctcgtc
     9301 ctgacatgcg gtctcgcctt atatgcacat ggagaacatg gtcactccag accaacacta
     9361 atcaacatta accctattca ctttcgttaa gcactttttg gtgggtccgt ttggatgcat
     9421 tgaataggtg cggactccac atggagacag aaagtgcttt accaaaggag catcctttat
     9481 gaataccaac gaagttagcg tcatcaacct tgaccagct
//
LOCUS       DADLZM010000146         9041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLZM010000146 DADLZM010000000
VERSION     DADLZM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.147"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNY73_003437"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNY73_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY8049322.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNY73_003438"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNY73_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNY73_003439"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNY73_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY8049323.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="JNY73_003440"
     CDS             complement(2403..2606)
                     /locus_tag="JNY73_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049324.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="JNY73_003441"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="JNY73_003441"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY8049325.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITHLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="JNY73_003442"
     CDS             complement(3802..4014)
                     /locus_tag="JNY73_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY8049326.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="JNY73_003443"
     CDS             4274..4582
                     /locus_tag="JNY73_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY8049327.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNY73_003444"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNY73_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY8049328.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNY73_003445"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNY73_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY8049329.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="JNY73_003446"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="JNY73_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY8049330.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="JNY73_003447"
     CDS             complement(8478..8981)
                     /locus_tag="JNY73_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8049331.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2240 a         2314 c         2149 g         2338 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccgc acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccaattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacattt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat t
//
LOCUS       DADLZM010000147         9011 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLZM010000147 DADLZM010000000
VERSION     DADLZM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.148"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(96..1472)
                     /gene="trkA"
                     /locus_tag="JNY73_003448"
     CDS             complement(96..1472)
                     /gene="trkA"
                     /locus_tag="JNY73_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY8049332.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1494..2783)
                     /gene="rsmB"
                     /locus_tag="JNY73_003449"
     CDS             complement(1494..2783)
                     /gene="rsmB"
                     /locus_tag="JNY73_003449"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY8049333.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2829..3776)
                     /gene="fmt"
                     /locus_tag="JNY73_003450"
     CDS             complement(2829..3776)
                     /gene="fmt"
                     /locus_tag="JNY73_003450"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY8049334.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3791..4300)
                     /gene="def"
                     /locus_tag="JNY73_003451"
     CDS             complement(3791..4300)
                     /gene="def"
                     /locus_tag="JNY73_003451"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY8049335.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4448..5572
                     /gene="dprA"
                     /locus_tag="JNY73_003452"
     CDS             4448..5572
                     /gene="dprA"
                     /locus_tag="JNY73_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY8049336.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5544..6017
                     /locus_tag="JNY73_003453"
     CDS             5544..6017
                     /locus_tag="JNY73_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY8049337.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6046..6588
                     /locus_tag="JNY73_003454"
     CDS             6046..6588
                     /locus_tag="JNY73_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY8049338.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6593..7165
                     /gene="tsaC"
                     /locus_tag="JNY73_003455"
     CDS             6593..7165
                     /gene="tsaC"
                     /locus_tag="JNY73_003455"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY8049339.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7170..7988
                     /gene="aroE"
                     /locus_tag="JNY73_003456"
     CDS             7170..7988
                     /gene="aroE"
                     /locus_tag="JNY73_003456"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY8049340.1"
                     /translation="METYAVFGNPIAHSKSPLIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7985..8242
                     /locus_tag="JNY73_003457"
     CDS             7985..8242
                     /locus_tag="JNY73_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY8049341.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8218..8772)
                     /locus_tag="JNY73_003458"
     CDS             complement(8218..8772)
                     /locus_tag="JNY73_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY8049342.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2256 a         2189 c         2248 g         2318 t
ORIGIN      
        1 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
       61 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
      121 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
      181 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
      241 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
      301 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
      361 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
      421 tgtggtgaaa ttgcaatatc gatcacgctc ccctgaacca gatccacata agcgcgacgc
      481 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
      541 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
      601 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
      661 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
      721 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgtttatac
      781 ggtttttcca gtcgctgtaa ttcactcatc accgcgcgga tatgctgtga agcggcaata
      841 aagaacactt catcaccagc ttcaacaatg gtcgaacctt gtggacgaat ggggcgatcg
      901 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
      961 agtgcattac caatcagcgg gccgccataa taggctttaa ctacagcgag gctgacttta
     1021 ccctcagcga agttcaccac ctgcaatgcg cctggatact caatcagtcg gtaaatatta
     1081 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
     1141 agcttatcgg catcgcgtac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
     1201 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
     1261 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
     1321 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
     1381 gtatcgacaa cagtaatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
     1441 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
     1501 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggctg attttgttta
     1561 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
     1621 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
     1681 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
     1741 tccgggatat cgcgatcgcg acgtaaccat ttaatatctg gatggcgacg aatcaccccc
     1801 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
     1861 gaagggtaac ggccatcacc ttgtttcacg gttgccttca taccaaggcg ttttaaattg
     1921 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
     1981 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc caagatgtgt
     2041 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
     2101 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
     2161 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccag caatgcaagc
     2221 cagctgtcgc gggaatgatg cgtacgatta atacgcagcc acattggcgg acgctggtta
     2281 ttggcttcgg cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
     2341 caggaaggat gcagataacg tgcatcacgg gtattaaact cggctaataa ctcttcttgc
     2401 cgacgctgga actggcgtaa tacaccgtta atcaaccctt taagttgcgg acgcttaatt
     2461 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
     2521 agttgataca aaccaaccat aatcaggtaa tgcacagtgc gctgtttgcc ggtcatcgga
     2581 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
     2641 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
     2701 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
     2761 cgtaaattac gttgtttttt cataagtata aaaatgctcg gcaagaccgg gcttagaaga
     2821 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
     2881 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
     2941 tcaccagtcg caacctgaat gccttgtttg ttggcttcaa ggatcgttcc tggtgcagcg
     3001 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggctgtcc ttcaatttcc
     3061 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg tgctgccgaa
     3121 agtgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
     3181 tcgtcctgaa cttctggttt cgccgtgccg tctgccaatt gtttcaacgt ggtgataagc
     3241 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
     3301 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
     3361 atggttacac cagtttctgc atcacccgcc catagtgagc gttggattgg cgcagcaccg
     3421 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
     3481 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcaaatcg
     3541 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
     3601 tcttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
     3661 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
     3721 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
     3781 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaattc
     3841 gttgttgttt cagcggtgac agataatcca taaacagttt gccaaccagg tgatccatct
     3901 catgctgaat acagatagct aacagaccct ctgcttccag ttcaaatggt ttaccgtcgc
     3961 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
     4021 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
     4081 ttaacaccag tcgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
     4141 tatcaacctg ggttgccgct aggccaatac cttcttctgc gtacatcgtc tcgaacatat
     4201 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
     4261 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
     4321 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
     4381 cttgattgac agcatcactg accaatcgca aagattgcta aggcttgctt atgacaggga
     4441 gataaggatg gtcgatacag aaatttggct gcgtttaatg agcatcagca gcttgtacgg
     4501 cgatgatatg gtccgtatcg ctcactggct ggcaaaacag tcgcatattg atgcggttgt
     4561 attgcagcaa acagggctta cattgcggca ggcacaacgc tttctttcat ttccacgaaa
     4621 gagtatcgaa agctcacttt gttggttgga gcaacccaac catcatttaa tccctgcgga
     4681 cagcgaattt tatcctcctc aacttcaggc gacgacagat taccccggcg cactgtttgt
     4741 tgaaggagaa ctgcacgcgc tgcattcatt tcagcttgcc gtagtgggga gtcgggcgca
     4801 ttcatggtat ggcgagcgat gggggcgatt attttgcgaa actctggcga cgtgtggagt
     4861 gacaattacg agtggactgg cgcgtggaat cgatggtgtg gtgcataaag cggccttaca
     4921 ggtaaatggc gtcagcattg ctgtattggg gaatggactt aatatcattc atccgcgtcg
     4981 ccatgcccga ctggctgcca gtctgtttga acagggtggc gctctcgtct cggaatttcc
     5041 cctcgatgtt ccaccccttg cttacaattt cccacgaaga aatcgcatta tcagtggtct
     5101 aagtaaaggt gtactggtgg tggaagcggc tttgcgcagt ggttcgctgg tgacagcacg
     5161 ttgtgcgctt gagcaggggc gtgaagtttt tgccttgcca ggtccaatag ggaatccggg
     5221 aagcgaaggg cctcactggt taataaaaca aggtgcgatt cttgtgacgg aaccggaaga
     5281 aattctggaa aacttgcaat ttggattgca ctggttgcca gacgcccctg aaaattcatt
     5341 ttattcacca gatcagcaag acgtggcatt gccatttcct gagctcctgg ctaacgtagg
     5401 agatgaggta acacctgttg acgtcgtcgc tgaacgtgcc ggccaacctg tgccagaggt
     5461 agttactcaa ctactcgaac tggagttagc aggatggatc gcagctgtac ccggcggcta
     5521 tgtccgattg aggagggcat gccatgttcg acgtactaat gtatttgttt gaaacctata
     5581 ttcacactga agctgagttg cgtgtggatc aagacaaact tgaacaggat cttaccgacg
     5641 caggttttga gcgagaagat atctacaatg ccctgctatg gctggaaaaa cttgctgatt
     5701 atcaggaagg gttggcagaa ccgatgcaac tggcctcgga tcctctctcc atgcgtattt
     5761 atacaccgga agagtgtgaa cgactggatg ccagctgccg tgggtttctg cttttccttg
     5821 agcagattca ggtgctcaac cttgaaactc gtgaaatggt gatagagcga gtgctcgcgc
     5881 tggataacgc agagttcgaa ctggatgatc tgaaatgggt gatcctgatg gtgttgttca
     5941 acatcccggg ctgcgaaaat gcgtaccagc aaatggaaga attactcttt gaagtgaatg
     6001 aaggtatgct gcattaatca tttctttaat tcagcataag ttgttatggc aaaatcagca
     6061 ctgttcacgg tgcgtaataa tgagtcctgc ccaaagtgcg gggctgaact ggttattcga
     6121 tccgggaaac acggtccgtt tcttggatgc tcacagtatc cggcgtgtga ctacgtccgt
     6181 cctctgaaat cttcagcgga tggacatatt gtcaaagttc tggaggggca ggtttgcccc
     6241 gtatgtggcg caaatctggt attacgccag ggacgctttg gtatgtttat tggttgcagt
     6301 aactaccctg aatgcgaaca taccgaactt atcgataaac cggacgaaac agcaattaca
     6361 tgcccccaat gtcggacggg ccatctggtc cagcgccgct cccgttatgg caaaacattt
     6421 cactcctgtg atcgctaccc ggagtgtcaa tttgccatta acttcaaacc aatagctgga
     6481 gaatgccctg agtgtcatta tccgctactc atcgaaaaga aaaccgcgca gggtgtaaaa
     6541 cacttttgtg ccagtaaaca atgtggaaag ccggtttcgg cggaataata acgtgaataa
     6601 taacctgcaa ggagacgcta tcgcagctgc gatagatgtc ctcaatgaaa aacgtgtcat
     6661 cgcctatcca acggaagccg ttttcggtgt cgggtgcgat cctgatagcg aaacagcagt
     6721 gatgagactg ttggagttaa aacagcgtcc ggttgataag gggctgattt taatcgcggc
     6781 aaattacgag cagcttaaac cctatattga tgacaccatg ttgactgacg tgcagcgtga
     6841 aaccattttt tcctgctggc caggtcctgt cacctttgtc tttcccgcgc ctgcgacaac
     6901 accgcgctgg ttgacgggcc gctttgattc gcttgctgta cgagtcaccg accatccgtt
     6961 ggtggttgct ttgtgccagg cttatggtaa accgctggtt tctaccagtg ccaacttgag
     7021 tggattgcca ccttgtcgaa cagtagacga agttcgcgca caatttggcg cggcgttccc
     7081 ggttgtgcct ggtgaaacgg gggggcgttt aaatccttca gaaatccgcg atgccctgac
     7141 gggtgaactg tttcgacagg ggtaacataa tggaaaccta tgctgttttt ggtaatccga
     7201 tagcccacag caaatcgcca ctcattcatc agcaatttgc tcagcaactg aatattgaac
     7261 atccctatgg gcgcgtgttg gcacccatca atgatttcat caacacactg aacgctttct
     7321 ttcgtgctgg tggtaaaggt gcgaatgtga cggtgccttt taaagaagag gcttttgcca
     7381 gagcggatga gcttactgaa cgggcagcgt tggctggtgc agttaatacc ctcaagcggt
     7441 tagaagatgg acgcctgctg ggtgacaata ccgatggtgt aggcttgtta agcgatctgg
     7501 aacgtctgtc ttttatccgc cctggtttac gtattctgct tatcggcgct ggtggagcat
     7561 ctcgcggcgt actactgcca cttctttccc tggactgtgc ggtgacaatc actaatcgga
     7621 cggtatcccg cgcggaagag ttggctaaat tgtttgcgca cactggcagt attcaggcgt
     7681 tgggtatgga cgaactggaa ggtcatgagt ttgatctcat tattaatgca acatccagtg
     7741 gcatcagtgg tgatattccg gcgatcccgt catcgctcat tcatccaggc atttattgct
     7801 atgacatgtt ctatcagaaa ggaaaaactc cttttctggc atggtgtgag cagcgaggct
     7861 caaagcgtaa tgctgatggt ttaggaatgc tggtggcaca ggcggctcat gcctttcttc
     7921 tctggcacgg tgttctgcct gacgtagaac cagttataaa gcaattgcag gaggaattgt
     7981 ccgcgtgaat caggccatcc agtttccgga cagggaagag tgggacgaga ataaaaaatg
     8041 tgtatgtttt cccgctctcg tgaatggtat gcaactgaca tgcgcgatct ctggcgagag
     8101 tctggcgtat cgctttactg gagatacgcc agaacagtgg ttagcgagtt ttcgtcagca
     8161 tcgctgggac ctggaagaag aagcggaaaa cttaattcag gaacaaagtg aagatgatca
     8221 aggctgggtc tggttaccct gatccagata ttcgtccttc catttcacgt aattattcgc
     8281 ggaatagcgt aacccagcct tctcttcatc acttaacggg cggatctgtt tgacggggct
     8341 accgagatac agatatccgc tctccagccg tttattttgt gggaccagac tacccgcacc
     8401 aatcatcaca tcatcttcta ctattgcgcc atcaagtaaa attgagccca tcccaaccaa
     8461 aactcgattg ccaatggtgc agccgtggag catcaccttg tgaccaacag tgacatcttc
     8521 gccaatggtt aatgggttgc catctgggtt gtacgaggat ttatgagtga catgcaacat
     8581 actgccatcc tggatattgg tgcgtgctcc gatctgtaca taatgtacat ctccacgaat
     8641 cacaacgagc ggccagatcc ccacatcatc agccagacga acgtcaccaa tcacgacact
     8701 gctatcgtcg atcattacgc gctgaccgat ttgtggaaaa agatcgcggt atgggcgtaa
     8761 aacatcagac atacttacct cagcaataaa tgatttacta atgactttgg gggcattatt
     8821 ggccttgtgc aagtctttta gtatgcaaaa aagcaccgtt ttgtgtgcga ttgcagcaaa
     8881 aagggtgaaa aaacaacaaa cagaaaaaaa gatcaaaaaa atacttgtgc aaaatattgg
     8941 gaaccctata atgcgcctcc gttgagacga caacgtgaaa cacttcacag gatggtcgga
     9001 acaacgaaga g
//
LOCUS       DADLZM010000148         9010 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.149, whole genome shotgun sequence.
ACCESSION   DADLZM010000148 DADLZM010000000
VERSION     DADLZM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.149"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            111..2998
                     /locus_tag="JNY73_003459"
                     /pseudo
     CDS             111..2998
                     /locus_tag="JNY73_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3108..3956)
                     /gene="alkA"
                     /locus_tag="JNY73_003460"
     CDS             complement(3108..3956)
                     /gene="alkA"
                     /locus_tag="JNY73_003460"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY8049343.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4090..5442
                     /gene="yegD"
                     /locus_tag="JNY73_003461"
     CDS             4090..5442
                     /gene="yegD"
                     /locus_tag="JNY73_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8049344.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5454..5675)
                     /locus_tag="JNY73_003462"
     CDS             complement(5454..5675)
                     /locus_tag="JNY73_003462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8049345.1"
                     /translation="MFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLH
                     APARTMPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(5871..7394)
                     /locus_tag="JNY73_003463"
     CDS             complement(5871..7394)
                     /locus_tag="JNY73_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAY8049346.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHGNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKARVAALDSLRQQLKKCPVSAMHVYPAHLTDCLWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQK"
     gene            complement(7391..8153)
                     /locus_tag="JNY73_003464"
                     /pseudo
     CDS             complement(7391..8153)
                     /locus_tag="JNY73_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
     gene            complement(8150..8809)
                     /locus_tag="JNY73_003465"
     CDS             complement(8150..8809)
                     /locus_tag="JNY73_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY8049347.1"
                     /translation="MSEQITFATSDFASNPELRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2197 a         2389 c         2357 g         2067 t
ORIGIN      
        1 accagacccg gtgacatcac cccccggatc atggacgact gggggcagta atcatgccgg
       61 gtctggctgg ctaacacccc attataaggg gtacgaataa agtaacgggg ctggttgtcc
      121 tgctgatgcc cggagacgat cctctcaggg catttttgat atgggtactg tcagaatcca
      181 tcggcgctct ggcactggtg ccgctgggat tgttatttaa accacactat ctgctgcgcc
      241 atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt agccatcaca ctgacgttaa
      301 gctggctttc gatgctgtat ctgccgtggc cttttacttt cattattgtg ctgttgatgt
      361 ggagcgccgt gcgcctgcca cgaacggaag cctttttgat cttccttacc acggtgatga
      421 tggtgtcgct gatgatggcc gcggatccct ccctgcttgc tacgccgcgt acgtacctga
      481 tgagccatat gccgtggcta ccgtttttgc tgatcctgct gcccgccaac atcatgacca
      541 tggtgatgta tgcctttcgt gcggaacgca aacacatttc cgaaagcgaa acccgctttc
      601 ggaacgcgat ggaatattcc gctatcggta tggcgttagt gggcaccgag ggacaatggc
      661 tgcaatccaa caaagcactc tgccagtttc tcggttacag tcaggaagag ctgtgcggac
      721 tcacctttca gcaactgacc tggccggagg atctcaataa agatctccaa caggttgaaa
      781 agctgataag cggtgaaata aacacctatt caatggaaaa acgctactac aaccgcaatg
      841 gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg ccacacggat ggcacgccgc
      901 tctattttat cgctcagatt gaagacatta acgagctaaa acgcaccgaa caggtgaatc
      961 agcaactgat ggagcgcatc acgctggcta acgaagcggg cgggattggc atctgggagt
     1021 gggagctgaa gccgaatatt tttagctggg ataagcggat gttcgagctg tatgaaattc
     1081 ctccgcatat caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg gaagatcgcc
     1141 agcatgccga aaaagtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat
     1201 tgccgtgaaa gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga
     1261 aggcgaagtc gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa
     1321 cgaggcattg tttcaggaaa aagagcgcct gcacattacg ctggattcca tcggtgaagc
     1381 cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagcgg cggagaagat
     1441 gagcggctgg acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac
     1501 ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc
     1561 gattgaacaa gatgtggtgt tgcactgccg gaacggcggc agttacgacg tgcattacag
     1621 tattacgccg ttaagtactc tggacggcag caatataggc tcggtactgg tgattcagga
     1681 cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact
     1741 gacgcatctc gccaatcgcg ccagttttga gaagcaactg cgtatcctgc tgcaaacggt
     1801 aaacagtaca catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt
     1861 gaatgacagc gccgggcatg cggcgggtga cgctttactg cgcgaactgg cgtcgttgat
     1921 gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc ggtggtgatg aatttggtct
     1981 actgttgcca gactgtaata tcgaaagtgc gcgttttatc gctacacgca ttatcagtgc
     2041 cgtgaatgat tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg
     2101 gattaccttg attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat
     2161 cgcctgttat gcctccaaaa atggtggacg gggccgggtg acggtttacg aaccacagca
     2221 agctgccgca catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa
     2281 agagaatcag ttgatgatga tcgcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg
     2341 taatttgtgg ctgatttcac ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca
     2401 aaaatttcgt cgtagcttca gcgatccggc acttagccat gctcttgacc gacgggtatt
     2461 ccacgaattt ttccagcagg ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct
     2521 ccccctttcc gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct
     2581 ggaaaatagc cctctaccac cacggttatt acatctgatt attccggctg aagcgatttt
     2641 agatcacgca gaaagcgtgc aaaaactgcg gctggcggga tgtcggatag tgctcagcca
     2701 ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct
     2761 acttgatggt gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac
     2821 gattattcag gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt
     2881 acccttagtg atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgatgtgat
     2941 tgccgatgcc caaccgctgg atttgctggt gaatagcagt tatttcgcga ttaactgaga
     3001 gagcgtttgc ccattgcctg atgcgatgct aacgcgtctt atcaggccta caaatcgctc
     3061 attccgcagg cgggataagg cgctcgcacc gcttccggcg accaacgtca tgcttcgtct
     3121 ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcca gggcttccag
     3181 cgctcggcat aacggcggat ttgtgccggt gtcatcccca gaaatcgctg tttaatcaga
     3241 taatcatccg gcagaaaaac atctttcgcc tgccagccgc gcaaggcgaa ataattcgcc
     3301 gtccagcgcc cgatacccgg aaaagtttgc agcgttttca tcgcctgctc cacatcgccc
     3361 ggtattgtca ttggtaaggt gccctccagc gccgcatttg ccagatgaat cagcgcctct
     3421 gcccgtttca acggcatacc taacgctttt aatgcctgcg ggtcggctgc tgccagccgc
     3481 tgaggcgtgg ggaagcagat atactccgga aaatcatcca gccgttcgcc ataaagctgt
     3541 gccactctgg cggtcaattt tgccgccatc gccacgctca ccagttggcc taaaatcgcc
     3601 cgcacgccct gctcaaaagc atcaacactg ccgggtaaac gcaatccggg ccgcgccgcg
     3661 cctaacttgc ccaacgcacc gttaacaatc tgcgggttac attgcagatc aagcaggcgg
     3721 ctcattttcg ccagacactc agcggcaacg ggttctaaac ctgcacttaa atttatgtgc
     3781 agagtatggc gggctatatc cggaatagca gtcaccacgc cgcgatattc gcccactgcc
     3841 agactacggg cataataact gtccgcgacc gtttccacac cactcaccgc acgggcggcg
     3901 agaaatccca acatccacga ccagtcatac ggcggctgcc agttcaggat gtacatcgca
     3961 tctccttatt catccgcttt cagcataaac gttattcaga cgttacgctt tgctttcata
     4021 ttccggttgt cgcgacggca acatttcgct aaagtcacgc cccttcttca ccggcatggg
     4081 gattatttcg tgtttattgg ttttgattac ggtacagcaa actgttcagt ggcggtcatg
     4141 cgtgacggta aaccgcattt gctaaaaatg gaaaacgaca gcacgctgct gccttcaatg
     4201 ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt accgccatca tgacgttccg
     4261 gcagacgacg atgaaacgca ggcgttgtta cgacgggcga ttcgttataa ccgcgaagaa
     4321 gatatcgatg ttacagcgaa aagcgtgcag ttcggtcttt cctcactggc acagtacatt
     4381 gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat cgttcctcgg tgccagcggc
     4441 ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat gttgcacatt
     4501 cgccagcagg cgcaggcgca gctgccagaa gcaattactc aggcggtgat tggtcgcccg
     4561 atcaacttcc aggggctggg cggcgatgaa gcaaacgccc aggcgcaggg gattctggaa
     4621 cgcgcggcga agcgtgccgg gtttaaggat gtggtattcc agtacgagcc ggtagcggct
     4681 gggctggatt acgaagccac cttgcaggaa gaaaaacggg tgctggtggt ggatatcggc
     4741 ggtggtacga ctgactgttc attgctgctg atggggccgc agtggcgttc gcgtctcgat
     4801 cgtgaagcca gtctgctggg tcacagtggt tgtcgtattg gcggtaacga tctggatatc
     4861 gccctagcgt ttaaaaacct gatgccactg ctgggtatgg gtggcgaaac cgaaaaaggc
     4921 atcgccctgc cgatcctgcc gtggtggaat gcggttgcca tcaacgacgt tcctgcacag
     4981 agtgatttct acagtagtgc caacggtcgt ctgcttaacg atctggtacg cgatgcccgc
     5041 gaaccggaaa aagtggccct gttacagaaa gtctggcgtc agcgtttaag ctatcgcctg
     5101 gtgcgtagcg cagaagagtg caaaattgct ctttcaagcg tagcggaaac ccgcgcctca
     5161 ctgccgttta tcagcaatga actggctacg ctgattagcc agcgagggct ggaaagcgcc
     5221 ctcagccagc cactggcgcg gattctggaa caggtgcaac tggccctgga taacgcccag
     5281 gaaaaaccgg acgttatcta cctgaccggc ggtagcgccc gttctccgct gattaaaaaa
     5341 gcgctggcag aacagttgcc gggcattccg attgcaggcg gcgatgactt tggctccgtc
     5401 accgccgggc tggcacgctg ggcggaagtg gtatttcgtt aaaagaaaat ggctcagcag
     5461 cggcttaaat cagcctgcgc ctgagccagt ttttctgccg cctgacgtaa cggttccatc
     5521 aacggcatgg tccgtgctgg agcatgaagg cggaatcgct gcaactctgt cgcgccgaca
     5581 gtcaacgtcg attccagccg atgtcgtttg gcggccattt tcgccattac cgcctgtctg
     5641 tctgctggcg ttaccgcttc gttcggcctg aacacaaaac ggcgttcaca gctcgctttc
     5701 cagtcgatga ccgcctgggt cagatgatca ccaaaccctt taacttgctt aacgccacga
     5761 cgggtaacac ctgctgctgt ttcaatacca aaagaccata acgccgcttt acgcgcaggg
     5821 ccaacgccgg gaatagaggc aacatcaata aaaaatccct ccagaaactt ctacttctgc
     5881 cgttcccttg cagtgtcatg aagtgctgcc agagcgagtt tctcttcttc cggtaacccg
     5941 agaatttcgt ccttcatttt ttcgagcatc gtccgtttgg cgataaaacc ttccaggccg
     6001 cctacctgct ggatctggcg gactaaatgg tcatagtcca ttttcgcacg attaaatgcc
     6061 tcacggcgtt gctgaacttc tgctttgtac gctttgcttg tcagactgcc aataatccag
     6121 atagccgcca gcacaggaac caaaataata taacgcggtt ctgcctgaag gccgcaaagc
     6181 agcagcgata atgctgacag tgcgatctca agcagaatga tgtattcgcg ccgtaacagg
     6241 cctaaaggaa gcggcctgcc agtcggttgg aaatgatcgg gtaatggcag ttgcaatgct
     6301 ggcggtgcta ctgacgccat caccatcgcc cagacttttg ccagcacaaa atccccgccg
     6361 gtggtaatga cctcttcgcc gagatcaata aaatagataa cgccttgatt atccagcgta
     6421 caccacaggc agtcggtcaa atgagcggga taaacatgca ttgccgaaac gggacatttc
     6481 tttaactgtt ggcgcagaga atcaagcgcc gctaccctcg ctttagccgt cggacgcccg
     6541 gttgccacgc cactttccgt gaatgcctgc tgaaacatgg cttcaacatc actcggtaac
     6601 atcgatagcg gaatcgatcg tggcggcggt tttaaaccac gtcgctgatt atctgacgcg
     6661 taggcataac ggaaatgggt aatatccgtc tccagcgcat tacccgcatc agagataagc
     6721 ggcacaccag aataaggatg ccggccacca aacaagacgt gaaaaatcag caacgcaagg
     6781 ccaaaattac cgtgattttc tgtgcgttca aagccgacaa atgacgacaa ggtttgtagc
     6841 tctggcggcg taaaatgcga cacgccgact tcgcataaat gcagtgtgcc attggcgtta
     6901 atctgaaagg agtcgctatc aatcaacacc actttgctgt cgcgacctac cataaaactg
     6961 ttctggttta cgtcacccac gacatgcccg tgctcgtgaa ccgtagcaaa agatgaagca
     7021 atattgcgcg caacatagag tagaaaatcc cacgcacaat gagggtaacg ttgacgacga
     7081 tgtgccgggc tatagatcat atgaatcggt tctttaccag aaacttttgg catcataaaa
     7141 ccgataactt ttccgcctcg tccaccgtga agcgttgcct gcggccaggc gacataattc
     7201 aacaattgcg cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt taaggcgggt
     7261 ggcggcgtgt gataaatctt ggcgacgcta tcgacaaact cattgatatc ataaaccgcg
     7321 ccttcaccgc ctttgcccag ttcacggcct ggcgtaacgc actcgccagt agcagtatat
     7381 aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt tttatcatca tccgtacgct
     7441 cgtttaccgc cgggctggac aaaaactgct ttaacaattc aggtaataaa tcgagttgtt
     7501 cctgcgttgc tgacgccagt ccattgaaaa acggggtaaa gaaaggcaca tgcggagaat
     7561 tatccaacat atttaacgcc agtcgctgga taccatccgt aaatgctgca actttatgca
     7621 cacgctcagt gctggtgaaa gtttccagtc tggaaacggc atcttcatcg gtaatgaagt
     7681 gcgtcatatt ggcatattca cccaccatcg gcgtaagcgg caattgcaga ccgtgaccaa
     7741 aatccaccac cacgccgcca tcgccaatct gcataatcaa ggtgccatta gccgatgaaa
     7801 tcaatcccag aaacgtacag gcaaaatcgc gaactgctaa ttctttggct tccgcctcgg
     7861 caataaagtc gctgacgaac ggtcagcaca atatccgtgg cgaggatatc attaagcccc
     7921 aattccccac cctgcacttt ttgcgacata taagccatcg cttcattgac ggcgagcatc
     7981 gcgccttcgc ccccctgcga aacgctacct gcgccatcag caaggaacat caccagtaat
     8041 ggctgctggt catttagcca ggcaacctgc atctggcagg catcctggca aggcaagtcc
     8101 gcgctgatgt gcgacgttcc aacagcggat gcataaacca gacgccagct cacactgacg
     8161 tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac
     8221 gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccttgc agaggcaaag
     8281 gctgacgaac gctgatttgt gccagagtct tcatatccgc cccctgaaca ccgatggaaa
     8341 agaaagcaaa ccttttatct tcttcccccc gaaacacttt gttggctgca gcctgccact
     8401 catcggttgg tgcaccatcg gtaatcagga aaatccacgg gcgataatac gaaataccat
     8461 ttgcacgata ctcacgtttc cgctcctcta ccatatccag ggctttggta atggccgcac
     8521 ccattggtgt atcgccctgg gcaaacagga tgggcggaaa aaaattagcg gcgctggtaa
     8581 agggttgttc cacatgcacc gggccgaacg tcacaatgcc gagttccact ctttttaaag
     8641 ccagcgaatc ggcaagcagc tcatcgcgaa aggtaaccaa tccggtgtta agttcattaa
     8701 taggtctgcc actcatcgag ccagaaacat ccagcagcag aatgcagggg caacgtagtt
     8761 ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata agaaatcctt
     8821 tcttagagaa ttattgaggt ccggtatcat accgactgca ataatgaaat aagagcaaac
     8881 gaagaaactt tcagaattag tcccggcggc gcagaaaatt ttgctattcg cctcgcaaga
     8941 acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag tgagggttag
     9001 ggagaggttt
//
LOCUS       DADLZM010000149         8993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.150, whole genome shotgun sequence.
ACCESSION   DADLZM010000149 DADLZM010000000
VERSION     DADLZM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.150"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNY73_003466"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNY73_003466"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY8049348.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKLWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNY73_003467"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNY73_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY8049349.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="JNY73_003468"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="JNY73_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY8049350.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /locus_tag="JNY73_003469"
                     /pseudo
     CDS             complement(5771..7252)
                     /locus_tag="JNY73_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNY73_003470"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNY73_003470"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY8049351.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="JNY73_003471"
                     /pseudo
     CDS             complement(7822..8133)
                     /locus_tag="JNY73_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNY73_003472"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNY73_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY8049352.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQFCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JNY73_003473"
     CDS             complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JNY73_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY8049353.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGATVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2253 a         2326 c         2133 g         2281 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccaga gtttggtacg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ctgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaacagag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg gggtcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaatcag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccctccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtcgct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctaccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctatgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaaa attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgtag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta tta
//
LOCUS       DADLZM010000150         8983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLZM010000150 DADLZM010000000
VERSION     DADLZM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.151"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..924
                     /locus_tag="JNY73_003474"
                     /pseudo
     CDS             1..924
                     /locus_tag="JNY73_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="JNY73_003475"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="JNY73_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY8049354.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNY73_003476"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNY73_003476"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY8049355.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="JNY73_003477"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="JNY73_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY8049356.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="JNY73_003478"
     CDS             4447..4806
                     /locus_tag="JNY73_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY8049357.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNY73_003479"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNY73_003479"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY8049358.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="JNY73_003480"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="JNY73_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY8049359.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="JNY73_003481"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="JNY73_003481"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY8049360.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2042 a         2175 c         2427 g         2339 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactgatat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagagttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtc
//
LOCUS       DADLZM010000151         8888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLZM010000151 DADLZM010000000
VERSION     DADLZM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.152"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..219
                     /locus_tag="JNY73_003482"
     CDS             <1..219
                     /locus_tag="JNY73_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY8049361.1"
                     /translation="ALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQ
                     LQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            246..1021
                     /locus_tag="JNY73_003483"
                     /pseudo
     CDS             246..1021
                     /locus_tag="JNY73_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(1085..1576)
                     /locus_tag="JNY73_003484"
     CDS             complement(1085..1576)
                     /locus_tag="JNY73_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY8049362.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1678..2232)
                     /locus_tag="JNY73_003485"
     CDS             complement(1678..2232)
                     /locus_tag="JNY73_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8049363.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2256..2993)
                     /locus_tag="JNY73_003486"
     CDS             complement(2256..2993)
                     /locus_tag="JNY73_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY8049364.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3048..3986)
                     /gene="ghrA"
                     /locus_tag="JNY73_003487"
                     /pseudo
     CDS             complement(3048..3986)
                     /gene="ghrA"
                     /locus_tag="JNY73_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            4220..4307
                     /locus_tag="JNY73_003488"
     tRNA            4220..4307
                     /locus_tag="JNY73_003488"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4254..4256,aa:Ser,seq:gga)
     gene            complement(4457..5299)
                     /locus_tag="JNY73_003489"
     CDS             complement(4457..5299)
                     /locus_tag="JNY73_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAY8049365.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELIDRLNDIIAKHYPD
                     MLAV"
     gene            complement(5384..5581)
                     /locus_tag="JNY73_003490"
     CDS             complement(5384..5581)
                     /locus_tag="JNY73_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY8049366.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(5593..6084)
                     /locus_tag="JNY73_003491"
     CDS             complement(5593..6084)
                     /locus_tag="JNY73_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049367.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(6081..6455)
                     /locus_tag="JNY73_003492"
     CDS             complement(6081..6455)
                     /locus_tag="JNY73_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY8049368.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(6502..6876)
                     /locus_tag="JNY73_003493"
     CDS             complement(6502..6876)
                     /locus_tag="JNY73_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY8049369.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(7039..7260)
                     /locus_tag="JNY73_003494"
     CDS             complement(7039..7260)
                     /locus_tag="JNY73_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY8049370.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(7323..7799)
                     /locus_tag="JNY73_003495"
     CDS             complement(7323..7799)
                     /locus_tag="JNY73_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY8049371.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7815..8300)
                     /locus_tag="JNY73_003496"
     CDS             complement(7815..8300)
                     /locus_tag="JNY73_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY8049372.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(8391..>8888)
                     /locus_tag="JNY73_003497"
     CDS             complement(8391..>8888)
                     /locus_tag="JNY73_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY8049373.1"
                     /translation="TLQETRGGSGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEG
                     AYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRR
                     EDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETL
                     LESMR"
BASE COUNT         2193 a         2285 c         2255 g         2155 t
ORIGIN      
        1 gcgttagata actttactca ggccatccag tcacaaattt taggtgggct actgtcgaat
       61 attaataccg gtaaaccggg ccgcatggtg accaacgatt atattgttga tattgctaac
      121 cgcgatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc
      181 caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc ataaggaaaa
      241 taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga tgcttaaccg
      301 ccccgcctaa agaagccgcc agaccgacat taatgcctcg tgctcagagc tacaaagatt
      361 tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac attcaggacg
      421 aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg
      481 ccagggctta caaaacctgc ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg
      541 cacggttgcc attaataacc gaatcccgct gcaatcttta acggcggcaa atattatggt
      601 tgaaggttcg attattggtt atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata
      661 ttttgggatc ggtgcagaca cgcaatacca gctcgatcag attgccgtga acctgcgtgt
      721 cgtcaatgtg agtaccggtg agatcctctc ctcggtgaac accagtaaga cgatactttc
      781 ctatgaagtt caggccgggg ttttccgctt tattgactac cagcgcttgc tggaagggga
      841 agtgggttac acctcaaacg aacccgttat gctgtgtctg atgtcggcta tcgaaacagg
      901 ggtcattttc ctgattaatg atggtatcga ccgtggtctg tgggatttgc aaaataaagc
      961 agaacggcag aatgacattc tggtgaaata ccgccatatg tcggttccac cggaatcctg
     1021 acagataaaa atgccggaat taataatccg gcatttactg tttaagcgaa agcgtgagtg
     1081 cccctcacgc tttatttttg atttcctgct cacggtgagc cgtctttgca ctacgcgccg
     1141 ccagccacag gccgctgttt ttcattgcgt aaccaaatac cagcccaagc gccagcgaag
     1201 gtaacaccag tttccagtca ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc
     1261 ccggaacaaa tgaaagcagc agctgtttgg cctgaataca catcagaaac gcgacaatac
     1321 cggttatgac ataaccgaga atttccagat gtggtgccag tgcactaccg taaataatga
     1381 ccatcgccca caccacgcca ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc
     1441 caccttgcgg gcaagcaaag taggccgtac agcccaggaa gcccgcccag cttagtaagc
     1501 ccagggaaac agccacccat ccccagatac cggagagaat gcctgttgtg attgcaatag
     1561 agagaagtat gttcatgccg cgcatgatag cagaaaacgc ggccatgaat gagatcgagc
     1621 acacatttta tgcaacaaca aattatctgt tgcagttatg agtgacgatg atcacattta
     1681 ctcttcagaa tcttcttcca gctcgtccca cattgcacta atggcatcac gggttagcgg
     1741 tgccatggtg cgccagaaag gcgtggttgc atgggcctcc actttgccga ggaacgcacc
     1801 acaccagggt aacagatact cactgaacag tgtttccagt gcttcgctct catcttccgt
     1861 tgactgatct tccagccagg aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt
     1921 atccgccagt ggcatccctc gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc
     1981 ctcaacccat gcgctacgat atggcggcac ggcacattca tcgccgataa acaacgcatt
     2041 gtaatcggca gagacttgcg tcatatcaca gcttttctgt aaacgcgtca gtaactcatc
     2101 ctgctccagt ggccagttcg cagccagttt cccctcacga atcagagtaa acagcggcac
     2161 cagtaaagga tcttgcggtt ggcggtaata gagcgaaccc agcacacgac agaggataga
     2221 aaactcgttc attaatatat ttccattact agtaattaaa ggtctgcaaa ttccgcaatc
     2281 ggtgccatac cgcgagattc aaggaagttc agtaagcggc gcggagaaac attcaaaatg
     2341 cgctctggcg gaaaatctac cgcgtcgagg attttaagac actcttcaaa ttcccccatg
     2401 gtaaacgcgg tgtgagaatc cgagcctaat gccacccaac ctccagcatc acgtaccgct
     2461 gcagctaccg cacgacagtt gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta
     2521 ttgatttcca gcgccacctg atgtttcgcg gctgcttcag caacggcttt aacatctatt
     2581 tcatatttcg gatttccggg atggcttatt atatgcacat tgccgctggc gatagtggcg
     2641 atcatcgctt gtgtattggt cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg
     2701 gcaataatca gatccagcga gtcaaacatt ttaccgctgc agtcaatttc accatcaaca
     2761 tttttaatgt tagcttcaat gccgcgcagg atccctaccc catcaaccac tcgcggccag
     2821 atacgcatgt taatgaagtg ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg
     2881 gtgatcgcaa aaagtttaat gcccttttgt ttggcctggg caatgtaatc actcaatgtg
     2941 ctatatgcat gtgtgctggc aacggtatgc atatgaaggt cgacgggata cataactctc
     3001 tcctgtattc aattcccgcc aaggatagca ggaatcctga tgctttatta gtagccgcgt
     3061 gcgcggtcga cttgcccgca gaccctctcc cctttttcga gctgggcaat agtgcgagaa
     3121 atgtactcca cagcttcagc gggacgggta atcgcggcga catgtggtgt tatcgtcacg
     3181 cgtggatgtt gccagagcgg actttcaggc ggtaagggtt cacgattaaa aacatccagc
     3241 attgcgcctt taactttccc gctatccagc gccgcgagca ggtcatcttc cacaacatga
     3301 acaccacgcg ccaggttgag gagatacgcg ccatcaggta atttttcgag taattgttga
     3361 ttaataatgc cgacggtttc aggggtattc ggtaacaaat taatcaatac ccgacattgg
     3421 ctcagaaatg cagacagttc ttcccgtccg gcaaagcttt gcacgccagg ccacgattta
     3481 cgggttcgac tccagcaacg cagcggaaag cgctaggttt gcagactctg agcaacttta
     3541 cagcccagta cgcctgcgcc caaaatgccg atggtaaaat cttcccgatg atattcaggc
     3601 agcggttgcc aatgcgaact attttgctgg atgcgataat catcaaaacg tcgaaaccaa
     3661 tgcagcacct gactgacagc atattcctgc atttgctcgc ccataccggt atcttccagg
     3721 cgaaaaagtg gaacagaagg gttcagcatt tcagggtgtg cctgtagctt gctcaaaata
     3781 gaatcaacac cggccccgag tgcgaacacc gctttaagat cgcgccctgc cagcatttca
     3841 acaggaggat gccagactaa aacataatca gcagagtcat tatctccgct tttccatgcc
     3901 ctgactcttg cctgaggaat agctttgcgc agtgcctcaa tccaccattg ggtatcgaac
     3961 gttgggtgat aaaagatgat atccatactg actcccgaaa agcgttgtgc gaaatttatt
     4021 cgcacttatc gttatgatct acaaaggcca ccagcataac aaatccgtgg tcggtggcaa
     4081 aaaaagcaga tttcgcttat taaaaccaca cattgattga agtttgaata aacgcgcgat
     4141 tctttcaaaa agtttgttga cctcaggtca tgatttccct aaattagcgc ccgttccagc
     4201 aagacaggaa cgacaatttg gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt
     4261 gtgtatacgg caacgtatcg ggggttcgaa tccccccctc accgccagat tataagcgca
     4321 tgttattgtt taacatgcgc ttttttattt tcccttccag acagtatgcc atcgggtatg
     4381 ccatctgaaa ccggtttata tgccccgcga tcatatctta tcggacaatt tccgatcttt
     4441 gagcatactg gcaaagctat actgccagca tatcaggata gtgttttgcg ataatatcat
     4501 tcagtctgtc aatcagttct ggttttctga acgtgatatg cgcggagccc ttcttaaagt
     4561 atctgatgct gaacatctca tcttcatagc tctctttacc ctgaacagac tgaatgtgat
     4621 catccagacg aagggtgatg tttccccggt tgtcggggat tggtttaccg ctgaacaggt
     4681 gcaacattct ttccagatcg gcgagtcggt cagcctgttg tccggtgata agatgaaatc
     4741 cccaccggtc ccatctcacc agattgttga cgataatttt actgccaaat ttacaggggc
     4801 aattggtttt gtaattccag ttcagccctc tgaagacgtt gatcacccct cgctcaaaca
     4861 cctcatcctt attctggtgc agttgttcaa atgtgctcag aatgttcgct tcgctgattt
     4921 ccggaaaatt atcgtactcc agtgaccggt accatgtatc acgggcctgc gtatccatta
     4981 atgacagcat cccggaacgt tgcatcaggt cacgccagat ttcgcgatca agattccggg
     5041 taatggcttt cattgctgtt tcgggtttct ccatcagcca gcagccgcag cgaaaatcct
     5101 gtttcatggc ccagtccggg gcagttttac ctccgatgct ttccgtcagc accgagattt
     5161 cagccagttg tcggataagt gtgtcgattt gcaacagtgt actctttcgt acagccagaa
     5221 tgtggcgaat gctggttgag cagataacgt ccgtatgccc gttgaccact tccggacggg
     5281 agattgtgat atctgacata atcgatattc ctttttgcag ataaatgaaa acgccagccc
     5341 tgtgcaggct ggcgtcagag gaatgaagtc tggtgagtga gcttcaccgg ttctggtgca
     5401 gaagctgcag atgacgcagg gtacggacat cctcccgggc ctgttcgata cagggcaaca
     5461 gtgctgctga atctgtttta tcctcatcgt tgtcaaagat aattcccgat ccgcagtcga
     5521 tattgtcctg cagccacgca atcagaatat ccagcgctgt ttccgtggtt aatgatttca
     5581 tgttgttcct ggttaccagt cgaaagtggg taaacccggc aggcattcag cgctggcatc
     5641 cagatgaatg agactgacgc cataacgccg gatgagtgtg acgaccagac gacggaacgt
     5701 ttttgacagc cccaggcgtt taagccgcag aaccgggtat gaccacgcat cggtacgtaa
     5761 cagatatccg ctacctgtaa aatgaatcca ttctgattca ccgaagtcac tggtctggtg
     5821 tgacagcgag tacaaccagg cgttgtccgt ttccgtgata tgtgcggtac tgcagcgtat
     5881 gccggtaagg gtagcaaatg gtggcgtgca ttctgctgag gtctggtcag attcatcaac
     5941 aacacgaagg gagtagccgt tatcgcagac catgttaatc agttcagcga gctcaatacc
     6001 atcgacgtca acgacaatgc gccccatgtt cagtgcctgt acgttaacgc tgtcggcttc
     6061 cagtgtcagg gccagtttca tcatttcgcc tccggatact tacccaaggt aatgttattt
     6121 accgttctgt aattgtcgcg ggtcatcagg ccggtcgccc ggcgagcccg gaggatatca
     6181 atactgttta ttaactgaga gcggggacag gtgctgaatc ccggctggtc ggtacgcacc
     6241 agcgcgtatt tttccacgag aaaattaact gcatcacaca gtgaaatgcc tgcctcaata
     6301 tgctgctcaa tcacacgttc atcggcaaac ggtgtgtcat tcagtgtgag gccataatgc
     6361 tggtccagca gtcggatgag cagtgtctgc cagatggtga cgggagacgg gcagcgagat
     6421 acctcccgta catgtgtgtc ggataatgtt ttcatcttaa gttctctgta actggtagtg
     6481 ctaaaggtga gagcttcggg gttatgaggt ggttgcgagt gctggtgtcg gataaacagc
     6541 cagataaacg taaccacagg agccgagggt gtcggcttcg caggtcagcc cttttgcatt
     6601 cagcgtgacg gtatgctgat atcggggatt cagttcaccg ctggtgagca tgagttccag
     6661 ttgtttcatc agcagcggaa aggcctggtc cagatggtat gcatctgcgt cgctgaagcg
     6721 gcctctgata ccggcgcggt cggcaaggta atgcaggcgg ttaccctctt gcaccagacg
     6781 agcgccaaaa cagggcctca ctgtgcaggg aagcccccac caggggcggt cgtgattgtc
     6841 atcgggatga gttatcccgg agagtttgtc tgacacgata aaatcctctc tgaaaagtgg
     6901 ttaagaatgg tttaacgatg acaggtcagc cggcgcgctg accggtttcg gtgaagatgt
     6961 atgcattgat gagaatggcc tcatccacag cggcgtctga ggtcatctgg tcgtactcgt
     7021 ttcccgggtg acggaacatc agttcagggt gacactcatc agccggacat acgggccaaa
     7081 actgtcctta cggcgttctg caaacacggc aagaacgccg ggaatatcct gcacttcacg
     7141 accagtatac gcttcagcac tgccgtgcca gcgatactta ccggtacaga acggaaaaag
     7201 acgggatgtc ggatgttgtt ggtgaatacg catggcttca ccacgggtga taattttcat
     7261 aatgggatac ctctgaagac agaagataaa agtgaaaaca ggtgtgatgt ggttgtgaag
     7321 ggttaaagca gaccatgttc ggcaaaggag aaaacctggt tgccaccgac tatcagatgg
     7381 tccggcaccc ggatatccac caggcccagt gcctgtacca gacgttcggt gataagccgg
     7441 tctgccttac tgggtgtgac ttcaccggac gggtgattgt gcgccagcac cacggcagcg
     7501 gcattgtggt acagggcgcg tttaatcact tcccggggat gaacttccgt acggttgatg
     7561 gtgccggtga agagggtttc accggcaatc agctgattct ggttgttcag atacagcacc
     7621 cggaactctt cccgctccag tcccgccatg ttcagaatca gccattcccg tgccgcacgg
     7681 gtggaggtga aggccacgcc gggttcatga agatggcggt ccagggtttg cagggcccgc
     7741 agaatgagac tgcgctcgcc gggcgtcatc tctccgggca gaaaggaaag ctgctgcatt
     7801 gtccctgcct ccattcagtc aatgatgcgc ataatggcgc tgcattccgg atgctgcagg
     7861 gcgtaatccc gcagccggta atagtggacc gtcatggcat aacactccgt acgacaggca
     7921 tgatgactgt acgtcatcag acaggcagca ataccggcgg cttccgggct catttcagcg
     7981 cggttgccgt tcatggcatt gaacagtacc catgtttcgt catcatcgtc atccggttcg
     8041 ggtgccataa atgccccgcc gttgttcagg gtgtacagat tccagatacc accgcagtag
     8101 tcttcgcaaa gacggtccat ccagccgaag acacggggct ccagggtcac ccactgtgga
     8161 atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt catcagggac gggagtggca
     8221 accagctgag gtgggttatt ccctgatgca gtggttacgg aaacagaagg tgtggtggaa
     8281 ttatgcgaaa cggttgtcat gaggttattc cttataaaaa gtaaatgaat ggaagaagcc
     8341 ccggggcagg aacagatgtg agtcaggact gcactttcag tgaaacggca tcagcgcatg
     8401 ctctccagca gcgtttctgc catcacccac aatgcgcgat tgagcttaat gtcggtatca
     8461 atgctgtgaa tggcccgggt atggatacgt tttccttttg cactgcgacc ggaaatcccg
     8521 cctttcagca tattctcctg gatggtctga taagcactcc acaggtcctt accgtaatcc
     8581 tcccggcgtc gtggtgtcag aatgtcggcg gtggtgacgg gctgatgttc gtcaccataa
     8641 cggtaagtca gtgccgcctg tgccagcgcc tggcgtgccg gtggcggcag gaccagcgac
     8701 tgcatggcat cacgcttctc ctctatccgg tcaaaaacgc ccaccacctc gtaagcccct
     8761 tcaatgactc tgtccaccac atttccccgg tgtggaacac gcaattctcc cagagactga
     8821 ccgcagacca gtccattggt acaaatggca cgaaaatatc ctgaaccgcc ccgggtttcc
     8881 tggagagt
//
LOCUS       DADLZM010000152         8860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLZM010000152 DADLZM010000000
VERSION     DADLZM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.153"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..1178)
                     /locus_tag="JNY73_003498"
                     /pseudo
     CDS             complement(1..1178)
                     /locus_tag="JNY73_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1831)
                     /locus_tag="JNY73_003499"
     CDS             complement(1388..1831)
                     /locus_tag="JNY73_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049374.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            2346..3188
                     /locus_tag="JNY73_003500"
     CDS             2346..3188
                     /locus_tag="JNY73_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049375.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            3295..3558
                     /locus_tag="JNY73_003501"
                     /pseudo
     CDS             3295..3558
                     /locus_tag="JNY73_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4185..4730
                     /gene="cobU"
                     /locus_tag="JNY73_003502"
     CDS             4185..4730
                     /gene="cobU"
                     /locus_tag="JNY73_003502"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY8049376.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4727..5470
                     /gene="cobS"
                     /locus_tag="JNY73_003503"
     CDS             4727..5470
                     /gene="cobS"
                     /locus_tag="JNY73_003503"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY8049377.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSCDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5482..6561
                     /gene="cobT"
                     /locus_tag="JNY73_003504"
     CDS             5482..6561
                     /gene="cobT"
                     /locus_tag="JNY73_003504"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY8049378.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6623..7558
                     /gene="ldtA"
                     /locus_tag="JNY73_003505"
     CDS             6623..7558
                     /gene="ldtA"
                     /locus_tag="JNY73_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8049379.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFASQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7614..7689)
                     /locus_tag="JNY73_003506"
     tRNA            complement(7614..7689)
                     /locus_tag="JNY73_003506"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7654..7656),aa:Asn,seq:gtt)
     gene            8016..>8860
                     /gene="nac"
                     /locus_tag="JNY73_003507"
     CDS             8016..>8860
                     /gene="nac"
                     /locus_tag="JNY73_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY8049380.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQA"
BASE COUNT         2257 a         1971 c         2257 g         2375 t
ORIGIN      
        1 cacaggtctg tcgccccttc agttgccagt tcgctgtctt tacagacgta atcttttttc
       61 atgcgaccga aggcttccct gacaaaatca tcgacctagg cttcatcaac aggaagggtt
      121 ccgtttttcc gtaactcatg aagcgcacgg ttagccaggt atgcctggcg tgaggatccg
      181 ggataaacgc gggaggcggg cagcacatcg ctgttcagca ttgccggtac cttctgacag
      241 actgcatcag ccccatcgcc attacggtct ttttcatcaa atttattgac cagcacaaac
      301 aggcggccga ctgacacatc agcaatggca ttaagctctt tacgaacctc ctcatcggct
      361 ttcgagttca tctgggtgta gtccatcacc gccagcaccg cagaggcttt ctgcagttga
      421 tcccgcatca tcacttccat ctgcggttgt ccggcttcat tagggccggg ggtatccacc
      481 agtgtgagtg ttccctggca ttcatccatc cccaccagat ggctgaattc cacgtcgatc
      541 accggcagtt tctgcacttc tgcgtattca tcaaaaggaa attcactccc cattgccgca
      601 gcaagacgaa ccagatcgtt taacgatgcc agtccggtaa agattccctc ctccccgtgg
      661 tattcatttt tgagccagcc atcatccgtc agaatatcca gcagttcgcg ggtatccccg
      721 gtttgctgca gggtctgtgc gacctgctgt cctgccgggg tggcgacttt ttcctgcagt
      781 gtgattaata aattgcggac aggctgaata tgttccagat ggagaaccgg ctcagttttt
      841 ccgggaacgt ggcggatgag cgtcggtaca gaggtcatgg ggcggttacg gttaggcaga
      901 atttcctgcc ccacaatggc gttgatggtg gttgatttgc ctgctttcat cgtgccgaca
      961 acagccagca ccatctccct gcgggcggtt ttgatttgtt cgccctccag ctccttaatt
     1021 cttttcagtg ctttctgttt atcaaaaagc tgcccgtttt cgttcttacc gtcagataac
     1081 acatccggct catcaaccat ttgccggagc agattgatgc tcagttgcaa aagccggtcg
     1141 gcttcatcac aaagcagggt gatgttcttt tcgtgcatta tttaaccttc ctgagacgca
     1201 ttttcatgac aaccacggag gaggccattt actacaagcg atcgtttata acaatcaata
     1261 taatagaaac gatcgtcata ttctattaat atgaaaaaca caacgcaagg gggacaaccc
     1321 gggtatggct gcgtttgcat tgtgttttca tagagttaaa aatctgtaac gacaacccgg
     1381 cacatcacta ccgggttaag aagaaaatcc agttccatac cgcgcgggcc acagagacca
     1441 cggtgtcacg aacggcgcgc agggccgtac ggacaacaga ggcaacacag acactctccg
     1501 ccgtgtcaaa tatccggtcc accgcaccgg taaactgttc acgggtctga gtgcggacag
     1561 actccgtgcg cagttcgtcc tgcaggcgtg tcatcagagg gatggcggca tgtccgggaa
     1621 gcactgtcat gagtgcactg accggggtga cctccccctg aaacagtgca ttacagagtg
     1681 aggatttacc ggccccgctt ttacccataa tgccaggcac gggttcgtga cgggggagtt
     1741 tgcgcagatg ttccaggatg tgacgggaaa tcgggtaagg cagggaggag agcggttttt
     1801 caattgcctc aatggcatca gacggattca tacatgtatt cccagtgaaa aaagacaaaa
     1861 ccccgtgcac cggaaaggtg gcgggggatt cacggagata atatctttct gaaattattt
     1921 tgaatgcagg tatatgtggc tgatttgtat cagctaatga gtacaggtct gaatctaatc
     1981 tgacagtccg ctttgtgcca ggagcggact gacctgctcc ccgatgatta atacacagct
     2041 atgctagtaa tgtctgtaga tcgctgtgtg ttgcatctat cggttgaact cacagtccaa
     2101 accagactgt cagctttgat tagattctac ctactcaacc tgtcagataa ggtctgagct
     2161 aatacatcta atatatggct ttgtgcaggg gacggctatc gcgaacgtta ggctaggtat
     2221 ctgaatacgc acagttcact tggttgtact tcttgcactg atctgctatc ccgcacagct
     2281 atttataggt aatcttcagt gtataatctg ccgacttatg gaagcttgta caacaggaga
     2341 aatatatgaa aaaagaattg tttggacgtt gcccgtttga tctcgacgaa gatgtaaaaa
     2401 aaatagccgc gataaataaa tatatccatg caaaatttga ggagcataaa aataaaatag
     2461 acaacttaaa gggtgtggaa caaaaattga tggtcttaca gtactttaat gtactgaatg
     2521 aacttagtaa tcaggctatc tcttctttga atataggagc cttttcagca tcggaagtat
     2581 tagctagggt tataatagaa caggctgcca atcaatttta tatagcaata gatgatggcg
     2641 aaaatgcaca agcattactc aaggctagta agaaattagt tcatagtaat ggaaaacggt
     2701 ggctggagtg tttaaaatcc aaaggaatga ctaatccagc agcaaatgaa aggattcgca
     2761 tgggaaaaga actaactgat ctatttaata gattgtggcc aaatacacct gaatatccag
     2821 gaaccagaaa actttttgaa ttaattggct gggaaacaca ctatcatgcc cattacgttc
     2881 ccctttgtga ttcaatacat acattctctg atgatatggt aaatatagta tctctatata
     2941 atgcaatcca aagtgatgaa actactgcta tcgaattcat tcttgcagtt aaacaagaaa
     3001 ataaacggct ggcaatttat aattttgtaa ttgctacggc ccttcggtgt gaggccttag
     3061 taaatgtatt taactcttta ggttatcagg atattataac agaaatgaca ccaacaatag
     3121 atacagtcaa ccagactata attcgatatg atgattttga tcattccaga attgatgatt
     3181 gtcaataatt aattgacaga atacgtacat gcgcaacaac tagtgatagt tgacttattt
     3241 tcgctcttga gagacaacca tactcaaatc tcccacattg caggagattt gaatatgaaa
     3301 acgtcaccgt ggaataaaga ccgtaccatc ggtcaaaaaa ggccacttca gatatctcat
     3361 atctggggga tccggattag acttgagttg gaaggtaaaa cacgcgatct agctctgttc
     3421 aacatggctt tggacagtaa gctacggggc tgtgatttgg tcaaactcaa agtatcagat
     3481 gctgcatatg gcagttctgt ctcaagcaga gcaacggtgt tgcaacagaa aaccggcagc
     3541 cctgtccaat ttgagatgcc tttaataaca catcttttaa atgttgaaga attatcccgc
     3601 ctgctgaaga acgttgctct gagtgtgata aaccttttta ttcttgatga tgtcagcgag
     3661 aagatggaat atatccccga ctgggtcgtg gttacccggt taaacatctg gtggcgctaa
     3721 taaatctggc aagtcacatt ctgttgaatg ctgtgacagt gccttaccga gagagcgaaa
     3781 ttgtggtgtt ctagtgcata gtcgccacca ttccggataa ttctattgcg ttgttttaca
     3841 agttctttta cagcgcagat ctgtcgctga cgttgaatca atagcctgga tttcagaaca
     3901 caatgcccgt ttgctggttg gtccggcagt agcattaatt cattatctga atgaaatgtg
     3961 tcaatccaca gaaaccgctg tcaatgacgc attttgtcgg acgttaacac gagaaagtct
     4021 ggctattccg taatgccatc ttatttcgta atggattgag aatgctggat ttttaattat
     4081 attacgcgca gtattaatcg cgtaaatgtt ttgcggtcag caacggtggg ggatccattg
     4141 ctctgttcaa tgaataccat catacatttg aatcaggagt cggtatgatg attttggtga
     4201 cgggaggcgc atggagcggg aagagtcgcc atgcagaggt gcttattggg gactcttcac
     4261 aggttctgta tatcgctacc tcgcaaatcc ttgatgatga gatggctgca cggatagaac
     4321 atcatcggca aagccgcccg gagcactggc gcacagtgga gcgctggcaa catcttgatg
     4381 aattaattca tgcagacatt aacccgaatg aggctgtgtt gctggaatgc gttaccacaa
     4441 tggtgactaa tctgttgttt gattatggcg gcgataaaga ccctgatgaa tgggattatc
     4501 aggcgatgga acaggcgatt aatgctgaga ttcagtcgtt gattgctgcc tgccaacgtt
     4561 gccccgcaaa ggttgtatta gtgactaacg aagtgggaat ggggattgtg ccggagagtc
     4621 gtctggcacg acattttcgt gatattgccg ggcgggtaaa tcagcagttg gccgctgcgg
     4681 caaatgaagt atggctggtg gtttcgggta ttggagtaaa aatcaaatga gcaaattatt
     4741 ttgggcgatg ctctcattta ttacgcgtct gcctgtaccg cgtcgctggt cccagggact
     4801 ggatttcgag cattattctc gcggtattat tacttttcct ttgattggat tattgcttgg
     4861 cgcgattagc gggctggtct tcatggtgct gcaggcatgg tgtggcgcac cactggcggc
     4921 actgtttagc gtacttgtgc tggcgctgat gaccggggaa ttccatctgg acggcctcgc
     4981 tgatacctgt gatggcgtat tttctgcacg tagctgcgat cgcatgttgg aaatcatgcg
     5041 tgatagccgt ttaggcaccc acggcggtct ggcattgatt tttgtggtac tggcaaagat
     5101 tctggtgtta agcgagttgg ctctgcgtgg cgaaccgatc cttgcatcgc tggcggcagc
     5161 atgtgcggtc agccgcggga ctgccgcatt attgatgtat cgtcatcgtt atgcgcggga
     5221 agaagggctt ggcaatgtgt tcattggcaa aattgatggg cgacaaacct gcgtcacact
     5281 cggcttagcc gctatttttg ctgccgtatt attgctgggt atgcatggtg tggctgctat
     5341 ggtggtgacg atggtggcga ttttcatcct cggtcagctt ctcaaacgta cgctgggcgg
     5401 gcaaacgggc gatacgctgg gagcagccat agagcttggt gaactggtct tcctgctggc
     5461 actgctataa agagacatga catgcaaata attgccgatt tactgaatac gatcccagcc
     5521 atcaattctg ccgctatgtc gcgtgcacaa cggcatgttg acgggttact caaacctgtt
     5581 ggtagcctag gaaagctgga ggcgcttgcc atacaactgg caggaatgcc gggggtgaat
     5641 ggcataccgc atgtgggcaa aaaagcggta ctggttatgt gtgccgatca cggcgtctgg
     5701 gaggaagggg tcgctatttc tccaaaagaa gtgacagcca tacaggctga aaatatgacc
     5761 cgtggaacaa ccggcgtgtg tgtgctggca gcacaagcgg gcgctaacgt ccacgtaatt
     5821 gatgttggta ttgatactgc tgagcctatc cccgggctta tcaacatgcg tgtcgcacga
     5881 ggtagcggca atattgcttc agctccggca atgagtcgcc gtcaggctga aaagttgctt
     5941 ttggacgtca tatgttatac gcgggagctg gcgaaaaacg gtgtcacgct gtttggtgta
     6001 ggtgaactgg ggatggcaaa cacgacaccg gcagcggcaa tagtcagcac aatcactggc
     6061 cgggatcctg aagaagtggt tgggattggc gcaaacctgc cgacagataa actggctaat
     6121 aaaattgatg ttgtgcgtcg ggcgattacg ttgaatcaac caaatcctca ggatggtgtt
     6181 gatgtcctgg caaaagtggg tggatttgat ttggtcggaa tagctggagt gatgttaggt
     6241 gctgcttcct gcggtttacc cgtgttgctg gatggatttc tttcttatgc tgctgcgctc
     6301 gcagcctgcc agatgtctcc tgcaatcaaa ccgtatctca ttccttctca cttgtcggca
     6361 gaaaaaggcg cgcgtatagc gctctcgcat ttggggctgg agccttatct caatatggat
     6421 atgcgtttag gtgaggggag tggtgcagct ctggcgatgt ccatcatcga agctgcttgt
     6481 gcgatataca acaacatggg cgaacttgct gccagtaata ttgttctacc ggggaatacg
     6541 acttctgatt tgaacagcta aaaacgaaaa gtctgataaa agctatactt atcggatagt
     6601 attcgcgact taggaaaggg acatgatgcg tcgtgtaaat attctttgct catttgctct
     6661 gctttttgcc agccaaaata gcctggcggt aacttatcca ttacctccag agggtagccg
     6721 tttagtgggg cagtcgctta ctgtaactgt tcctgatcac aatactcagc cgctggagac
     6781 ttttgcctca caatacgggc aagggttaag taatatgctg gaagcgaatc cgggcgctga
     6841 tgttttttta ccgaagcctg gctcacaact caccattccg cagcaactga ttttgcccgc
     6901 cactgttcgt aaagggattg tggttaacgt cgctgaaatg cgtctttatt actatccgcc
     6961 agacagtaat acagtggaag tctttcctat tggtatcggc caggctgggc gagaaacccc
     7021 gcgtaactgg gtgactaccg ttgaacgtaa acaagaagca ccaacctgga cgccaacgcc
     7081 gaacacccgg cgcgaatatg cgaaacgagg ggagagtttg cccgcatttg ttcctgcggg
     7141 gcccgataac cccatggggc tgtacgcgat ttatattggc aggttgtatg ccatccacgg
     7201 taccaatgcc aattttggta ttgggctccg tgtaagtcag ggctgtattc gtctgcgtaa
     7261 tgacgatatc aaatatctgt ttgataatgt tcctgtaggc actcgtgtac aaattattga
     7321 tcagccagtg aaatacacaa cggaaccgga tggttcaaag tggctggaag ttcatgagcc
     7381 gctgtcgcgc aatcgtgcag aatatgagtc tgaccgaaaa gtgccattgc cggtaacccc
     7441 atctttgcgg gcgtttatca acgggcaaga agttgatgta aatcgcgcaa atgctgcgtt
     7501 gcaacatcga tcgggaatgc ctgtgcaaat tagttctggt tcaagacaga tgttttaaga
     7561 gcgttggtaa ttagaaagta aaaagcctgc tcgaaagcag gcttttttga atttggctcc
     7621 tctgactgga ctcgaaccag tgacatacgg attaacagtc cgccgttcta ccgactgaac
     7681 tacagaggaa tcgtgtgaac ggggcgcata ttaatggcag tgcatggtga tgtcaaaggg
     7741 tgaaatgcat ttttttgttt gtttgctgcc aatttcaaca aagtggtgag ctttcaatct
     7801 tattgggtaa tgaaccatcg tggtgcatac cctcctttta tagggcaggt gaatgcgact
     7861 gctgataaaa agggccaatg acgaatctat cataatctct tatttgacag ttggttagct
     7921 tgcgcatcaa cgccaaaata gaactggcag gcatcttgca tactggttgt aagtaatggc
     7981 ggcacttggg ccgattctta aaaaccggag gcaacatgaa cttcagacgc ctgaaatact
     8041 tcgtaaaaat tgtagatatt ggtagcctga cccaggctgc tgaagtattg catatcgcac
     8101 aaccagcgct cagccagcag gttgccatac tggaaggtga gttaaatcaa cagcttttga
     8161 ttcgcacaaa acggggcgtt acgccaacag acgccggaaa aattctctat acccatgcac
     8221 gggccatttt acgtcagtgt gagcaggccc aactggcggt gcataacgtt ggtcagtcat
     8281 tatcggggca agtctcgatt ggctttgcgc caggaaccgc tgcgtcatcc atcaccatgc
     8341 ccttattaca ggcggttcgc gctgaattcc cggaggtcgt tatctatctt catgaaaata
     8401 gtggcgcagt gcttaacgaa aaactgataa atcaccaact cgatatggcg gtgatttatg
     8461 agcattcccc tgtggctggg gtatccagtc aggctttgct gaaagaagat cttttcctgg
     8521 taggaactca agattgcccg gggcaaaacg ttgatgttaa tgctattgcg caaatgaacc
     8581 tttttctccc cagtgattac agcgctgtta gacttcgtgt tgatgaggct ttttccctac
     8641 ggcggctcac ggcaaaagtc attggtgaaa ttgagtctat tgccacgctt accgcagcga
     8701 ttgccagcgg catgggcgtt gcagtattac ctgaatcggc tgcgcgttcg ttatgtggcg
     8761 cagtaaatgg gtggatgtca cgcattacca ctccttcaat gagtctctct ttgtccttaa
     8821 atttacccgc cagagcaaac ttgtcaccac aggcacaggc
//
LOCUS       DADLZM010000153         8771 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLZM010000153 DADLZM010000000
VERSION     DADLZM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.155"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JNY73_003508"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JNY73_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY8049381.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="JNY73_003509"
     CDS             complement(984..1814)
                     /locus_tag="JNY73_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY8049382.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2024..3037
                     /gene="intI1"
                     /locus_tag="JNY73_003510"
     CDS             2024..3037
                     /gene="intI1"
                     /locus_tag="JNY73_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY8049383.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3240..3590
                     /locus_tag="JNY73_003511"
     CDS             3240..3590
                     /locus_tag="JNY73_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY8049384.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3716..4276
                     /locus_tag="JNY73_003512"
     CDS             3716..4276
                     /locus_tag="JNY73_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8049385.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4279..7245
                     /locus_tag="JNY73_003513"
     CDS             4279..7245
                     /locus_tag="JNY73_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY8049386.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7312..7689
                     /locus_tag="JNY73_003514"
     CDS             7312..7689
                     /locus_tag="JNY73_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8049387.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7890..8549)
                     /locus_tag="JNY73_003515"
     CDS             complement(7890..8549)
                     /locus_tag="JNY73_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY8049388.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2480 c         2348 g         1993 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc ccaactttgt tttagggcga ctgccctgct gcgtaacatc
     1921 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1981 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     2101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg
     2161 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     2221 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     2281 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     2341 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     2401 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg
     2461 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat
     2521 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc
     2581 cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg
     2641 ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc
     2701 ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt
     2761 cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga
     2821 acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc
     2881 cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt
     2941 ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc
     3001 gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg
     3061 gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt
     3121 cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct
     3181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa
     3241 tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg
     3301 gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg
     3361 gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct
     3421 gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gccggaaggg gccgagtacg
     3481 tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga
     3541 ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct
     3601 aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat
     3661 tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac
     3721 tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact
     3781 ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg
     3841 tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag
     3901 catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca
     3961 acgcggcgtg catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc
     4021 gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat
     4081 ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag
     4141 gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga
     4201 gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt
     4261 gagaacggat cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc
     4321 ctgctggcgt tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc
     4381 gacctctcga tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag
     4441 ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc
     4501 ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc
     4561 cagcgggagc agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg
     4621 cccttcacca tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa
     4681 accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc
     4741 attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac
     4801 cggcgcatct acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac
     4861 gatctgctca agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct
     4921 ccggccaagc caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag
     4981 gcactcgatc tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt
     5041 gcccgcgagg gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc
     5101 tacgccactc tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc
     5161 gacctgcacg accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag
     5221 cagttccagg cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt
     5281 caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc
     5341 atgtcctggg attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat
     5401 gacttcgatt tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg
     5461 gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc
     5521 attgaggtgc tgcgcggcat gaacaccgac aacgcccgca agctgccagc cgatgcaccg
     5581 accggcttca tcaagccgcg ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg
     5641 cgctactacg aactgtgcgc gctgtccgag ttgaagaact ccctgcgctc gggcgacatc
     5701 tgggtgcagg gttcacgcca gttcaaggac ttcgaggact acctggtacc gcccgagaag
     5761 ttcaccagcc tcaagcagtc cagcgaattg ccgctggccg tggccaccga ctgcgaacaa
     5821 tatctgcatg agcggctgac gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg
     5881 gcagccaacg acctgccgga tgccatcatc accgagtcgg gcttgaagat cacgccgctg
     5941 gatgcggcgg tgcccgacac cgcgcaggcg ctgatagacc agacagccat ggtcctgccg
     6001 cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt ggacgggctt cacccggcac
     6061 ttcacgcact tgaaatcggg cgatctggcc aaggacaaga acctgttgtt gaccacgatc
     6121 ctggccgacg cgatcaacct gggcctgacc aagatggccg agtcctgccc cggcacgacc
     6181 tacgcgaagc tcgcttggct gcaagcctgg catacccgcg acgaaacgta ctcgacagcg
     6241 ttggctgaac tggtcaacgc tcagtttcgg catccctttg ccgggcactg gggcgatggc
     6301 accacatcat catcggacgg acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg
     6361 cacatcaacc caaaatatgg cagcagccca ggacggactt tctacaccca catctccgac
     6421 caatacgcgc cattccacac caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg
     6481 ctcgacggcc tgctgtacca cgaatccgac ctgcggatcg aggagcacta caccgacacg
     6541 gcgggcttca ccgatcacgt cttcgccctg atgcacctct tgggcttccg cttcgcgccg
     6601 cgcatccgcg acctgggcga caccaagctc tacatcccga agggcgatgc cgcctatgac
     6661 gcgctcaagc cgatgatcgg cggcacgctc aacatcaagc acgtccgcgc ccattgggac
     6721 gaaatcctgc ggctggccac ctcgatcaag cagggcacgg tgacggcctc gctgatgctc
     6781 aggaaactcg gcagctaccc gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc
     6841 cgcatcgagc gcacgctgtt catcctcgac tggctgcaaa gcgtcgagct acgccgccgc
     6901 gtgcatgccg ggctgaacaa gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc
     6961 aaccgccttg gtgaaatccg tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc
     7021 ctcaacctgg tgacggcggc catcgtgctg tggaacacgg tctacctgga gcgtgcggcg
     7081 catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc tattgcagta cctgtcgcca
     7141 ctcggctggg agcacatcaa cctgaccggt gattacctat ggcgcagcag cgccaagatc
     7201 ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg cttagcgtgc tttattttcc
     7261 gttttctgag acgaccccta ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat
     7321 tatctctgta aaaagttatt caaccggctt tccggtaccc tggtgatcag agcgcgctgc
     7381 ggcaataaca ttactggcct ggcatgttgc aatattcttt atccctcgcc ccgatacagc
     7441 ggtcagctgc atattaaaga gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt
     7501 aaagcgataa tgcgctttat agcccggctt gcgcttgaac aggaatgcct tagccttagc
     7561 tggaacgctg aaaaatccaa ccccggcgct aaccgttttt atcaggctct gggaggcaga
     7621 ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca
     7681 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     7741 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     7801 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
     7861 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     7921 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     7981 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     8041 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     8101 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     8161 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     8221 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     8281 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     8341 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     8401 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     8461 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     8521 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     8581 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     8641 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     8701 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     8761 caaagtgcgt c
//
LOCUS       DADLZM010000154         8592 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLZM010000154 DADLZM010000000
VERSION     DADLZM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.156"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..323)
                     /locus_tag="JNY73_003516"
     CDS             complement(<1..323)
                     /locus_tag="JNY73_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8049389.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(372..800)
                     /locus_tag="JNY73_003517"
                     /pseudo
     CDS             complement(372..800)
                     /locus_tag="JNY73_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(800..1507)
                     /locus_tag="JNY73_003518"
     CDS             complement(800..1507)
                     /locus_tag="JNY73_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY8049390.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1522..2199)
                     /gene="ydjY"
                     /locus_tag="JNY73_003519"
     CDS             complement(1522..2199)
                     /gene="ydjY"
                     /locus_tag="JNY73_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY8049391.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2204..2914)
                     /locus_tag="JNY73_003520"
     CDS             complement(2204..2914)
                     /locus_tag="JNY73_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY8049392.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3081..3887)
                     /gene="xthA"
                     /locus_tag="JNY73_003521"
     CDS             complement(3081..3887)
                     /gene="xthA"
                     /locus_tag="JNY73_003521"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY8049393.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4333..5553
                     /gene="astC"
                     /locus_tag="JNY73_003522"
                     /pseudo
     CDS             4333..5553
                     /gene="astC"
                     /locus_tag="JNY73_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5550..6383
                     /gene="astA"
                     /locus_tag="JNY73_003523"
                     /pseudo
     CDS             5550..6383
                     /gene="astA"
                     /locus_tag="JNY73_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
     gene            6379..7308
                     /locus_tag="JNY73_003524"
                     /pseudo
     CDS             6379..7308
                     /locus_tag="JNY73_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            7305..>8592
                     /gene="astB"
                     /locus_tag="JNY73_003525"
     CDS             7305..>8592
                     /gene="astB"
                     /locus_tag="JNY73_003525"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY8049394.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2002 a         2208 c         2358 g         2024 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgtcaaaatg cgcagccagt cgggtacgca gggcatcatc gactgttggc
      421 ggtgtgacgc ttgcggcttc ggtgtactcc agcgccagcc gttcttcatc gctaaagagc
      481 gggctttgcc gccagtcagc cactgccagc agtttatcgg tgctgccggt acgctcggcg
      541 actttcatac tggtgatatc cacgcaaaac tcacacaggc acatttgcgc aatgcgcgcg
      601 ctgacaagcg atcgcactac cggatcgagc ggtgagcgtt tgcgctccag ccacccaaca
      661 aacattgaca ccaaataaaa gataaacggg atccgccccc accagcgaat cgggcttagc
      721 acctcgccgt aatggcgacg atgcagccac gcctgcggac gtaaaatgaa aggagttttg
      781 ctaagcggcg gtaaacccat cagtcattcc tcttctggcg ctcaagccat attttcttcg
      841 ccataaaaat caccacggtc agggcaaaca gaataaacag ttcggtgaca aaccagaaag
      901 taccgcctgt taacatgctg cccgcccagg aataaacaat agtcgccggt aattgaccaa
      961 gcccggtggc gataaaaaac gagcgaaaac gtattgaagt cagaccggca gcatagctga
     1021 ttggatcgaa agggacaaaa ggcaataacc gacagaccag gatggtgtgt ttgccgtagc
     1081 gagtgaaaaa gccgtccata ctgtcaagca cggttttgcc ggttaatttt tccaccactt
     1141 cgcggcccat cactctggcg ataaaaaagc acagcgccgc gccggccatc gaactggtcc
     1201 acgacagcaa gccgccccag aacgcgccaa acagcgacgc attggcaaag gtgatcaaaa
     1261 acgcaggtag cggtgcagca atggcctgta aaatcatcaa caagaacgag acaactgccg
     1321 ccagtgcgcc gtaagactga ataaagcgtt ctataccctg ttggtccacg gcggcaaacg
     1381 ccgcaacgct gcgattgata aactcatgga cgccgggaag cagcgcccat gcgagcagca
     1441 tagcgaacaa caggatgatg agggttatac ggtagtacca gattttacgc gattgtatct
     1501 tcatcatcct ttatcctggc tttattcggt gattttaaag gtaaccgttg ccagcgtgtt
     1561 atccgccggg agaactgagg cattgccttt gaatttcact tcgccgcgtt tttccaccgc
     1621 accataagtg tatgttgcat tgctgacgat gccgaccggg cagctatcca gacacaccag
     1681 gcaacccgtt ttcttctctt ctgctgccgt taaattaccg ccaaagcgca tgtccagttt
     1741 cttgccatta ctgtcaacaa tcacttcatc gaaggaatac gcttttgccg ccccttgcca
     1801 gttgaccgaa atatccagtt tgctgcctgt gacatgagtc gtttctttat tatccatcgt
     1861 catgttttcg cccggcgtac cacctgcctc tttcagggct tcataaaatg ctttcggggt
     1921 cgcataagcc ataaacagcg atttatgtcc gttggagcca tctttaaaca caataccgtg
     1981 acgagtgtcg tcggtgagga aacgaccatt aatttgcacc agcatggtta ccgtatggtt
     2041 attggcatcg acgcgcagcg gttggctgtt cgaaagtccg tcgtattcca cttttgccgc
     2101 cgcattctct ttttgatcgc agccgctaag cccggcaaca gccagtaagc agagtgcagc
     2161 cagtcctttt ttccatgaca ctgaataatg ttgcgacatc gttctatcct tcattttttg
     2221 ggtgagtaag tgggctgcgg cgcgaagcag acagatccac atgtttgtgt cgggcgtaga
     2281 gttttgcgag ctggatgaga ataaacagcg ccaggcccgc cagacagagt tgtaaaataa
     2341 agcgcagcgt aatgccttca ttggcgagat cgcttgccat cacggtataa ataacaatac
     2401 cgggcagggt cgtgagtgcc gaaataaggg tataaggcca gaaggcgatg gtggttaatc
     2461 cgtaagcgta attttgaata ttgtaaggaa acaacgggat taagcgggtc agaataagaa
     2521 aatcaatacc gttacgcgca atgccttttt caatggcctg gaaggtatgg ctatggccca
     2581 cgtatttcag cagtaaatca cgtcccagcc agcgcgccag caggaatgag cacgaggagg
     2641 ccagcgttgc ggcaattaat gagagtagtg tccctaagag cgggccaaaa actattcctc
     2701 cagcgatcac cagtatgctt cctggtaaca gaaagagggg ggcattgatg aataacagaa
     2761 tatagaggtt ataaccgaac aatccgctct ggcgaatgag tgtctgtaag tggggtaaat
     2821 cggtgagcag atcgaataaa ccgaaagcgt ggatagcgta aatgaccagc gcaaaaataa
     2881 gacaggcaaa cagaaagtta cgctcagcgt tcatcgtgga gtttcctata tcaaacttga
     2941 taatagagaa gtggtcagac aactaaaaac gcagtgacgg tataacaaag gacggcaggc
     3001 aacaaatcat tactgacagt ttgttgattt ttctcaaggt taattctcct gacccagttt
     3061 gagccaggag agctgctaaa ttagcggcgg aaggtcgccc agacgggggc gtgatcggac
     3121 ggtttttcca tgctgcgaat ttcatagtcg atgccggttt ctacgcaaca ttctgccagc
     3181 ggttggctgg cgagcagcag gtcgatgcgc agaccacggt tatcgtcaaa accttttgag
     3241 cggtaatcaa accatgagaa acgatctgct gtttgcggat tagcatggcg gaaggtatcg
     3301 accacccccc agctcatcag cctgtccatc cattcgcgct cttccggcag gaaagagcat
     3361 ttaccggtac gcagccagcg cttacggttt tcttcgccaa tgccgatatc cagatctgta
     3421 gggctgatat tcatatcgcc cataatcagt accggattat cacgtttgag ttcggtttcc
     3481 aggtagtttt gcagattctg ataaaactgc gcttttgccg ggaattttat cggatggtcg
     3541 cggctttcac cctgcgggaa gtaaccgttg atcacggtga cataacccag cagtgagggg
     3601 atttccgcca taataatccg ccgctgcgcc tcttcgtcgt caccgggaaa gccgcgacgc
     3661 acggcaatcg gcgtctcttt ggtcagcagc gccacgccat aatggccttt ctgcccgtga
     3721 taaaacacgt tgtagccgag cttcgccacc tcttcgagcg gaaacatatc gtcatgaact
     3781 tttgtctcct gcaggccaat cacatccggt tggtgctttt cgacgatggc ttcaagctgg
     3841 tgaggtctgg cgcgcaggcc gttgatatta aaagagacaa atttcatagt cgctgccatt
     3901 tattcagata aagagtgcgt ggatggtagc agaatttcgc gttgcttacc gcctgttgtt
     3961 aagtgatttg atcgatggtt gttaatgatg tcaacgatgg cgcaaaaaat gcccgctttt
     4021 ggtgcgcgct gcgtcagaat ggcgcagtaa tttccagtaa atttcgtcaa tgatcacaat
     4081 tccagaatta tattttactt ctgcataaca ttgcgttttt tattcttttt attcacccac
     4141 atcgcaaacg tattcacttt atatgcactt taaatgcata tggtttggtt ataactcctt
     4201 gatttccagt tagcctccgc cgtttatgca cttttatcac tggctgacac gaaccctgca
     4261 atctacattt acagcgcaaa cattacttat tgttaacata caaataacga attatttact
     4321 gtagaggtcg ctatgtctca gccaattacg cgtgaaaact ttgatgaatg gatgatacct
     4381 gtttacgctc cggcaccctt tataccggta cgtggcgaag gttcgcgctt gtgggatcag
     4441 caggggaaag agtatatcga cttcgcgggt agcattgcgg tgaacgcgct gggccatgcg
     4501 catccggaac tgcgtgaagc gctgaacgaa caggcgagta agttctggca taccggcaac
     4561 ggttacacca acgagccggt actgcgactg gcgaaaaaat tgatcgacgc cacgtttgcc
     4621 gatcgcgtct tcttttgtaa ctccggtgcg gaagccaacg aagcggcgct aaaactggcg
     4681 cgtaaattcg ctcacgaccg ctacggcagc cataagagtg gcatcgtggc gttcaaaaat
     4741 gcgtttcatg gtcgcacgct gtttactgtc agtgcgggtg ggcagccagc ctattcacag
     4801 gattttgcgc cactgccggc ggatattcgt catgctgcat ataacgatat taactctgcc
     4861 agcgcgctga ttgacgacgc cacctgtgcg gtgattgtcg agcccatcta gggcgaaggc
     4921 ggtgtggtgc cagccagcaa cgcgttttta caaggtctgc gtgaattgtg tgaccgccac
     4981 aatgccttgc tgatttttga tgaggtacaa accggcgtcg ggcgcactgg tgagctgtat
     5041 gcctatatgc actacggcgt gacgcccgat ctgttaacca ccgccaaagc gctgggcggc
     5101 ggtttcccgg tcggtgcgtt gttggcaacc gaagagtgcg cccgcgtgat gaccgttggc
     5161 actcatggca ccacctatgg cggtaatccg ctggcctcgg cggtagcagg caaagtgctg
     5221 gagctcatca acacaccaga gatgcttaat ggcgttaaac agcgtcacga ctggtttgtt
     5281 gagcgtctta atactattaa tcaccgctat ggtttgttca gtgaagttcg cggcttaggt
     5341 ttgttgattg gctgtgtact gaatgccgat tacgccgggc aagcgaaaca gatctctctg
     5401 gaagcggcga aagcaggcgt gatggtactg attgcgggtg gcaacgtggt gcgttttgcg
     5461 cctgcgctca atgtcagcga agaagaggtg acgaccggac tggatcgctt tgcagctgct
     5521 tgcgaacact ttgttagccg aggttcatca tgatggtcat ccgtcccgtt gagcgctcag
     5581 atgtctcggc gctgatgcag cttgccagca aaacgggcgg cggcctgacg tcgcttcccg
     5641 ccaatgaagc cacgctttcg gcgcgtatcg aaagggcaat caaaacctgg caaggcgaac
     5701 tgcctaaaag cgagcagggc tatgtgttcg tgctggaaga tagcgagaca ggcaccgtgg
     5761 cggggatttg cgctattgag gtggcggttg ggctgaacga tccctggtac aactatcgcg
     5821 tcggcacgtt ggttcacgcc tcaaaagagc tgaatgtcta taacgcattg ccgacgctgt
     5881 ttcttagtaa cgatcacacc ggcagcagcg agctgtgcac gctgtttctc gacccggact
     5941 ggcgcaaaga gggcaacggc tatttgctgt cgaaatcgcg ctttatgttt atggcggctt
     6001 ttcgcgacaa gtttaatgac aaagtggttg ctgaaatgcg cggggtgatt gacgaacacg
     6061 gctattcacc gttctggcaa agcctcggta aacgcttctt ttcgatggat tttagccgcg
     6121 ccgattttct ctgcggcacc gggcaaaagg catttattgc agaactgatg ccgaaacatc
     6181 cgatctatac ccacttttta tcccaggaag cccaggacgt catcggtcag gtacatccgc
     6241 aaaccgcgcc tgcccgcgcg gtgctggaga aagaaggttt tcgttaccgc aactatatcg
     6301 acatcttcga cggcgggcca acgcttgagt gtgacatcga ccgcgtgcgc gccatccgta
     6361 aaagtcggct ggtggaagtg gcagcaggct ggcttgccgc cgggcgtgct gaacctggtg
     6421 cagggcgggc gtgaaacggg tcaggcgctg agtgcgctgg aggatctcga cggtttgctg
     6481 tttaccggta gcgccaatac cggctaccag ctgcatcgcc agctctccgg tcagccggag
     6541 aaaattctcg cccttgagat gggcggtaat aatccgctaa ttatcgatga ggtggcggat
     6601 atcgacgcgg ctgtccatct gaccattcag tcggcgtttg tcacagccgg gcaacgctgc
     6661 acctgcgccc gccgtttatt gctcaaaagc ggagcgcagg gcgatgcgtt tcttgctcgt
     6721 ctggttgccg tcagccagcg attaacgccg ggcaactggg atgacgaacc gcagccgttt
     6781 attggcgggc tgatttctga acaggccgca cagcaggtgg ttactgcctg gcagcaactg
     6841 gaagcgatgg gcggacgaac cctgcttgcg ccgcgcttat tacaagcagg gacatcgttg
     6901 ctgacaccag ggatcattga aatgacgggc gttggcggtg tgccggatga agaggtgttc
     6961 gggccgcttt tgcgcgtctg gcgttatgat acttttgatg aggcgattcg gatggcgaac
     7021 aacactcgct tcggactctt ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa
     7081 ctgttgctgg aggcgcgggc ggggattgtt aactggaaca aaccgcttac tggtgctgcc
     7141 agtaccgcgc cattcggcgg cattggtgct tccggtaacc atcgccccag cgcctggtat
     7201 gccgcagatt actgcgcatg gcctatggcg agcctggagt cggactcgtt aacattgccc
     7261 gccacgctta accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag
     7321 ttaatttcga cgggctggta gggctgacgc atcattacgc gggcctgtcg tttggtaatg
     7381 aagcctctac ccgtcaccgt tttcaggtgt ctaacccgcg actggcggca aaacagggct
     7441 tactgaaaat gaaagccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg
     7501 agcgtccgtt tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac
     7561 tggaaaaagt tgcacgccag gcaccgcact ggctttccag cgtcagttcc gcttcgccaa
     7621 tgtgggtagc caatgcggca acgatcgcgc catctgccga tacgctggat ggcaaagtgc
     7681 atctcaccgt tgccaacctg aacaataaat ttcaccgttc gctggaagcg cccgtcactg
     7741 aatcgctgtt aaaagcgatt tttaaggacg aagagaaatt tagcgtccat tcggcgttgc
     7801 cgcaggtagc gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt
     7861 acggtgaacc gggtatgcta ctttttgtct acgggcgaga agaaggcaat gatacccggc
     7921 cttcccgtta tccggcgcga cagactcgcg aagccagcga ggcggtggca aggcttaatc
     7981 aggtgaatcc ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagagcg
     8041 tttttcataa tgacgtgatt gccgtgagta accgccaggt gctgttttgc caccaacagg
     8101 cgttcgctcg ccagtcacag ttactggcaa acctgcgggc gcgggtgaac ggttttatgg
     8161 cgatagaagt tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta
     8221 acagccaact gttgagccgc gatgatggtt ccatgatgtt ggtgctgcct caggagtgtc
     8281 gggaacacgc cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta
     8341 gcgaactaaa agtctttgac ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc
     8401 tgcggttgcg ggtggtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga
     8461 tgaacgatac cttgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgtc
     8521 ttactgctgc cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg
     8581 tattgagcca at
//
LOCUS       DADLZM010000155         8452 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLZM010000155 DADLZM010000000
VERSION     DADLZM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.157"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..161)
                     /locus_tag="JNY73_003526"
     CDS             complement(<1..161)
                     /locus_tag="JNY73_003526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049395.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCA"
     gene            complement(196..1128)
                     /gene="narH"
                     /locus_tag="JNY73_003527"
     CDS             complement(196..1128)
                     /gene="narH"
                     /locus_tag="JNY73_003527"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY8049396.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKYIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDLYRVGSLTAAACHGLQLPL"
     gene            complement(1125..4758)
                     /locus_tag="JNY73_003528"
                     /pseudo
     CDS             complement(1125..4758)
                     /locus_tag="JNY73_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            complement(4840..6228)
                     /locus_tag="JNY73_003529"
                     /pseudo
     CDS             complement(4840..6228)
                     /locus_tag="JNY73_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
     gene            complement(6552..7883)
                     /locus_tag="JNY73_003530"
     CDS             complement(6552..7883)
                     /locus_tag="JNY73_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY8049397.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYTNL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            complement(7993..8394)
                     /locus_tag="JNY73_003531"
     CDS             complement(7993..8394)
                     /locus_tag="JNY73_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY8049398.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
BASE COUNT         2135 a         1973 c         2089 g         2255 t
ORIGIN      
        1 tctgcgcagc attcaacggc atcgtcaaaa ctatcaaagc gcagaacttc tgcgtcttct
       61 tcgtcaaaaa aatcatcttc gtgaagcttc acgacatagc ggggaagttt gcttctttga
      121 gaggcgggtt tacgtttacg gggtttagct gaacgggcca tataaccacc tgaaagacaa
      181 tgacattgcc tgtttttata acggtaattg cagaccatga caagccgcag ccgtcaggct
      241 gcctactcga tagaggtcaa cttcgcgctc ggtgctcgcc gcttcctcga tgcggtcggc
      301 gtcgtaaagc agcacgccca gataccggat gcgacccacg caggtttctg agcacacggt
      361 gggttggcct gactcaattc gcggataaca gaagatatat ttttctgact tgccgctttt
      421 ccagttgaag tagatttttt tgtacggaca accgctaatg cacaaacgcc agccacggca
      481 tttatcctga tcaatcagca caatgccgtc ttcttcgcgt ttgtagatag cgccgcttgg
      541 gcaggtcgcc acgcagctgg gattgaggca gtgttcgcac aggcgcggca ggtacatcat
      601 gaaggtgttt tcaaactgcc cgtacatctc cttttgcatg gcctcgaagt tgcggtcgcg
      661 ggcacgtttt tcaaactcgc cgcccagcag ttcttcccag tttgggcccc agatcacttt
      721 gtccatccgt ttgccgtcaa tcagtgaacg cgggcgagca gtagggatat gtttgccttc
      781 cggtgtgcta tgcaaatgtt cgtagtcgaa ggtgaaaggt tcgtagtaat catcaatctg
      841 ctgcggcacc accgggttgg cgaagatttt ggttattacg cccatcttgc tgcccagacg
      901 cggacggatc ttgccattca catcgcggac ccagccgcct tgccactctt cctgatcttc
      961 ccagtttttc ggataaccaa tgcccggttt ggtttcgacg ttgttaaacc atgcgtactc
     1021 catgccttcg cgtccggtcc agacgttttt acaggtcacc gaacaggtat ggcagccgat
     1081 acatttatcg aggttaagca ccatgccgac ttgcgaacgt attttcattt tttcgcctcc
     1141 tgtacctgat cgcgaccttc atcatccagc cagttaacgt tcttcatctt gcggatcatg
     1201 atgaattcgt cgcggttcga gccgacggtg ccgtagtagt taaagcccca ggccagctgc
     1261 gcgtaaccgc caatcatatg cgtcggtttc gggcaaacgc gggtgaccaa gttatgaatg
     1321 ccgccgcgca tgccagttac ttccgaacca ggaatattca taatgcgttc ctgggcgtga
     1381 tacatcatgg tcatgcccgg cggtacacgt tggctgacca ccgcgagaga gcgtcagcat
     1441 tagcaggttt tcactgtagg ttgagtgaat gccccatttc tggtgcggcg tcaggaagtt
     1501 aagtgctttt tcctggaagc cgtttggcgg tatctggcgc atctcactga cgctacgggt
     1561 gtcgataggc gggcgataag ccaccaggct ttcaccaaaa gcacgcatcc acggatgatc
     1621 ctgatagagc tgctggcgtc ccgacagcgt gcgccacgga attaactcat gaacgttggt
     1681 gtatcccgcg ttataggata cgtgatcgct ttccagaccg gaccatgtgg ggctggagat
     1741 aattttacgc ggctgcgcct gaatatcgcg aaagcgaatc ttctcgtcct ctttgtgcag
     1801 cgccagatgg gtatgttcgc gcccggtgat ctcgcccagc gcctgccacg ctttgactgc
     1861 aacatgaccg ttggtttccg gtgccagtgc cagaatcact tctgaagcgt caatggcggt
     1921 gtcaatcagc ggacgcccct gtgccgggcc atcacgcttg gtgtaattga gtttgccgag
     1981 gaaatcgatt tcatcctggg tattccacga aatcccttta ccgccattgc caagtttgtc
     2041 catcaatggc ccgagcgagg taaagcgttc atacgtagca gggtagtcgc gctccaccgc
     2101 cacaatattc ggcgcggttt tacccgggat cagatcgcat tcgcctttgc gccagtcgag
     2161 cacttcacac ggctgtgaga gctctgccgg agagtcatgc agcagtggtt gtaataccac
     2221 gtcggtttct ttgccaagat ggcccacgca cacttgcgaa aatgctttgg caataccttt
     2281 gtagatttcc cagtcgctgc gtgattccca ggccggatcg accgccgcag aaagcggatg
     2341 aataaatgga tgcatatccg aggtgttcat atcgtctttt tcataccagg tggcggtggg
     2401 caggacgata tcggagaaca ggcaggtact ggacatgcgg aagtcgagcg tcaccagcag
     2461 gtcgagcttg ccttcaatcg ccgcagtttg ccactcgact tcttccggtt tgatcccgtc
     2521 gctggcaccg agttcctcgc cctgaatccc gctttcggtc cccagcagat acttctgcat
     2581 atactcgtgg cctttgccgg aggagccaag caggttagag cgccagacaa acaaattacg
     2641 cggatgattg ctgccgctat ctggttgttc gcaggccata cgtaaatcgc ccgatttcag
     2701 cgcctgggcg gtaaattctg tgggggataa tccggccttg tcggcttcag ctttaatccc
     2761 gagcgggtta cgccccagct gcggcgcaga aggtagccag cccatacgtt cggcgcgaac
     2821 gttgaaatca atcagatgac cgctgtactt actggcatcg gcgagcggtg aaagtaactc
     2881 ctgagcagag actttttcat agcgccattg gctggaatga ttgtagaaaa acgaggtgct
     2941 gttcatctgg cgcggtggtc ggttccagtc gagcgcaaag gccagtggca accagccggt
     3001 ttgtgggcgc agtttttcct ggccgacata gtgtgcccag ccgccaccgc tttgcccgac
     3061 acaaccgcag aagatcagca tattgatcat cccgcggtag ttcatgtcca tgtgatacca
     3121 gtggttaaca cctgcgccga ggataatcat tgagcgccca tgcgttttat gggcggtatc
     3181 ggcaaattcg cgggcgatgg tttcaatata ctggcgcggt acgccggtaa tttgctcacc
     3241 ccaggctggg gtgtacggtt tgatttcagc gtaatctttc gcgctgtttt catcttccag
     3301 cccgcgatcg aggccgtaat tcgccagtac caaatcataa acgctgacca ctggacaggt
     3361 gttgccatcg actaacgtca ggtttttaac gggcaattgg cgcaccagca ccggattgtg
     3421 ttttacgctg cgaaaatgcg gattttcaat gccgccaaag taggggaagg ccacgcctgc
     3481 aacagcgtca tgttgaccga gcagggttaa cgacaattcg gtttccgtac cggcggcaat
     3541 ggattccaga ttccatttgc ctttttctcc ccagcggaaa ccaatcgaac cgttcggcac
     3601 taccaattca cctgcggtat taactgctac ggttttccac tgcggattgt tgctttcgcc
     3661 cagtccatcc accaggtcag atgcgcggat catgcgcccg ggaacgtagc taccatcgtc
     3721 gcgaggctcc agcattacca gcatcggcat gtcgctgtag cggcggcagt agttgataaa
     3781 gtagtcgctg ggattatcga gatgaaactc ttttaaaatc acatgcccca ttgccatcgc
     3841 cagggcgcta tcagtgcctt gtttcggtgc cagccactgg tcgcacaatt tggccacttc
     3901 agagtagtca ggggtaatgg cgatggtttt agtgcctttg tagcgtactt cggtaaagaa
     3961 gtgggcgtcc ggcgtacgtg tctgcggtac gttagacccc caagcgatga tgtagctgga
     4021 gttataccag tcggcagatt ccggtacgtc ggtttgctcg ccccaggtca tcggcgaggc
     4081 gggcggcagg tcgcaatacc agtcgtagaa acttaaacag gtgccgccaa gcagcgacag
     4141 ataacgcgtt ccggcggcgt aagaaaccat cgacatcgcc gggatcggcg agaaaccggc
     4201 aacgcgatcc gggccgtagg ttttgatggt ccagacgtta gcggcggcaa tcagctggtt
     4261 tagttcctgc cagttggagc ggataaaccc gccgcgccca cgcacttgtt tgtagctcag
     4321 gcacttttgc ggatcgttca taatcgatgc ccacgccagt accggatcgc tgtgttgttt
     4381 gagggcttcg cgccacagtt caatcagtcg tttacgaatg agcgggtatt tcaggcggtt
     4441 agcgctgtaa agataccagg agtaacttgc gccacgcggg cagccgcgag gttcatgatt
     4501 gggcaggtca gggcgagtgc gcgggtagtc ggtctgttgg atttcccagg tcaccagacc
     4561 atttttaacg tagattttcc agctacagga gcctgtacag ttaacgccgt gggtggaacg
     4621 cacgattttg tcgaactgcc aacgctggcg atagctgtcc tcccagtcgc ggttgctatg
     4681 catcacctgt ccgtgaccat cggcaaaggt ttcgcccttt tgtttgaagt agcgaaagcg
     4741 atccaaaagt ttactcatga catgactcct gctccaggaa tgaattggga tctgatgcct
     4801 tgtcggatgc gacacggttg tacatcaggc atcgatctct tatttttggc tgaacttccg
     4861 ccgaccataa accagccagg tcagcagcac acaaacgatg tagaagatta aaaacacttt
     4921 catcgcgccg accggagagc cggtcatatt gagcgacatg ccaaatgcct gcggaataaa
     4981 aaagccgccc actgcgccaa tggctgagat aaagcccagc gctgccgccg tttcggtgac
     5041 ggcttctctc tgagcttgct catcactacc gcctttcatc ttcacccgat aaatggttat
     5101 ctggcgaaag atgacggcga tcatctggaa agtagaacca cttcccagac ccgcggtcag
     5161 aaacagcccc ataaatacgg cgtaaaaggc gatgaaatta ccggagcctg tgcccggtaa
     5221 ggtaaggaac agcagggcac tgaaaatcgc cataaaaata aagttgatca acgtcacccg
     5281 cacgccgccg aatttatcgg aaatagcacc accaaccgac cgcgcgatgg caccaataaa
     5341 tgggccaaag aacgccaggc gcagaatatt cacatccggg aactgggttt ttgccagcat
     5401 ggcaaaaccc gcagaaaaac cgataaacga accgaaggtg gcaaggtaaa gcaggctcag
     5461 cagccagaga tgcaggcgtt gtaagacagg gagctggtcg gcaattgagg cgcgtgaact
     5521 ggcgatatca ttcatccctg accaggcggc gatcgtggca atcgccagta gcggtaccca
     5581 aatccatgcg gcattcgcca gcgacatcac cgaaccgtcg gcctgcggta cgccattgac
     5641 gccgagaaag gcaaatacag gtacaaaaat gaccagcggt gcaaccagct gcattacact
     5701 tacacctaag tttcctaatc cgccattaat cccaagagcg ctcccttgtt tggcttttgg
     5761 aaagaagaaa ctgatattgc ccatgctcga agcaaagttt gcacctgcaa aaccgcatag
     5821 caaagcgata acgataaata tcccaaaagg agtattcgga ttttgcacgg caattccgag
     5881 ccagacgcaa ggaataatca ggattgcagt actaaaaacc ggccattggc gtccaccgaa
     5941 tataggcacc ataaaggagt agggaacacg caataatgcg ccagaaacgg agggtaatgc
     6001 ggttaataaa aagagttgat cggtagtaaa attaaaaccg attttattga gattaacggt
     6061 aactgcgcta aatagcatct agacacagaa ggcaagaagt agacaactga ctgatatcca
     6121 gaggtttctt cgagcaatat gttttccttt attttcccag aaggccggat tttctggttt
     6181 ccagtcgcgt aaaagataac gactattttt ctcattttgc agtgccatat tgttcctcac
     6241 atgcacacat tggtaatgaa aaaagacaaa acaggaggta aggcgcaata gccagttatt
     6301 agaattaagg atgaattggg tgaagtgctg attgaaagaa tagataagaa agcgtaaccg
     6361 cgggggcaga atggggatta agtagccaga tatgtgttac taaatgtaac taacaagcta
     6421 accttcaaac gggggttaat ctttgaacgt atgtcatgtt tcaacccttc agatcgtgaa
     6481 tctaaagggt tacatattaa ctatattctc aacaagacca gacacttata gaattagtga
     6541 tgatttattc actaaaaagg ccatgaattg gaggaaaacg atctgtccag aaagtctatc
     6601 ggttcctctc ttattgcctg attgtaataa tcgttattat agtaagtatt gtttatttca
     6661 tctaaggtca tgtttttaac ttttatattc ctctggttta caacttcctc tgtcttgtaa
     6721 gtgtgtatat tatatggggt atatgctttt ttaatagtat agtgaaacaa ttcgttatcc
     6781 acatctattc cgtcagagtg aacttcaaat gcaccaggcg aaagaattgc caggccagta
     6841 ttaagcttaa gtgctgggga aaacaaatta aataacatga gctgcatagt agggcaattg
     6901 ttcttcccag aaacatttaa aaaatttaag tttttgctct gttttattat ggggaataaa
     6961 gttccaatat cggatgtatt attagtatca atgtgggtta ttttattatt gtccagtctt
     7021 aaacttttga tagatggaag ataagtcagg aaatcaagat ttgtatattt caactggttg
     7081 ttagcagcgt ttaatgtctg aacattgcgg aagttgctat agaatatttt aatttcagaa
     7141 agcaaattgt tgttaataag taacgtattc aagttgggaa ataagacatt gtgtaaactt
     7201 gttagtttat tatgggatag atccagcagt aagagttcat ttttattacc tgcaacaata
     7261 tcagttaatt gattatggct gagattcagg tattgcagcc attcgcaaga ttctaattgc
     7321 acaaactcta gttgattatg tgccgcacta aaataagtaa ctgatgaaag cctatctata
     7381 tcaatatttc tgagagcatt attattcata gatatatgtg taatacttac atttctgcct
     7441 tgaggaaaat ttattgactc aagagcatta ctattcagac ttagctttac gagtctatcg
     7501 tagttggttg catcaaaatg tgtgagttgg ttattatcaa gattgataga ggtgaagttt
     7561 tgcagccctg gtggttgagt ggttaaatta agtcctgata aatcaatggt cacttcatta
     7621 gtgtcatcaa ttatatttct tactgcagtc cagtactgca tttgtgcaag tggatcattg
     7681 ttgatgccgt taactaatac atgccaaaga tgttttgatg atattagttg atcaatgtca
     7741 ttggttggca gtaaattgtt attgcttaag tttaaatatt ccagttctgt agtgtttttg
     7801 gcgtttaaat gtgcaatatg gttgtcgttt aaagtgattg ttttcatctt gggatgattg
     7861 tgtaatataa ggtcagtgat catattgtga tgggcactta actcaatcag agattcttgc
     7921 cgggagacat ccagcgatgc tagcttgtta taatcaagat taagaaattt cagtttataa
     7981 ctatcagaaa tatcattttt ttaggctgtt attttgcgct tccagattaa ccaatgaatg
     8041 ctgatttttt aaatctaaac tttccagatt gttatgatca atataaatgt tttccagttt
     8101 tggttctttt ggaaaggtta tcgtgttaat tttattaacg ctgaggttga tattaacgag
     8161 actggcaatg tcagctaagt caagacaagt tagacaattg ttttccagat ttaacgtatt
     8221 aactgtcgag gggataggcg gtaattcact caaatcaaga tatgacaaat caagagatgc
     8281 catgttcgaa ttgtaagctt ctttaattcg ctccacggca attgctcttt cttcaccatt
     8341 attaaccgat ttattacccc attgttctag tcgttcacaa aaattgctgg agatggtttc
     8401 tttgttaggt aatgcgttat taagttgata actggcatta ttttttacac aa
//
LOCUS       DADLZM010000156         8320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.158, whole genome shotgun sequence.
ACCESSION   DADLZM010000156 DADLZM010000000
VERSION     DADLZM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.158"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            334..3183
                     /locus_tag="JNY73_003532"
     CDS             334..3183
                     /locus_tag="JNY73_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8049399.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDIEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3283..5211)
                     /gene="dhaR"
                     /locus_tag="JNY73_003533"
     CDS             complement(3283..5211)
                     /gene="dhaR"
                     /locus_tag="JNY73_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY8049400.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATMTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5430..6500
                     /gene="dhaK"
                     /locus_tag="JNY73_003534"
     CDS             5430..6500
                     /gene="dhaK"
                     /locus_tag="JNY73_003534"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY8049401.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGR"
     gene            6511..7143
                     /gene="dhaL"
                     /locus_tag="JNY73_003535"
     CDS             6511..7143
                     /gene="dhaL"
                     /locus_tag="JNY73_003535"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY8049402.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7154..>8320
                     /gene="dhaM"
                     /locus_tag="JNY73_003536"
     CDS             7154..>8320
                     /gene="dhaM"
                     /locus_tag="JNY73_003536"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY8049403.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2069 c         2075 g         2002 t
ORIGIN      
        1 cccataacta attaggatta ttacaaaaaa tcttcaccaa attttttaac cgctgtttgt
       61 tgtaatattt ttgccatatt catagtaacg aattggaata agtaacgaca acttagggaa
      121 taactcgata taaactcacc tttgtaaggg gggtgttaaa tatatattaa ttcctgtgtt
      181 cctgattttg cccggtaata gagaattatt ctcagagaag ctcatcatga ttgcaccata
      241 cagatatcat gatgataaga ggatattatt cattgactta aacaaaatga caaaatccat
      301 agacccatag cctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat
      361 accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa
      421 tttggcgcta ttggtttgaa tgcaattatc ttttcccccc tgctgatagc tgctgataca
      481 ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac aggagatacc
      541 gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat
      601 atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga
      661 atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt
      721 ggtcaaacct ctgccatcgg cattaactta attggtgact atacccatgc tgacttaggc
      781 acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta
      841 acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat
      901 ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag tacaggtgtt
      961 tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac
     1021 ctgacaatcg atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt
     1081 gtcgatctcg gaacaaacag taccattaaa accaatggcg ataatgctca cggcctctgg
     1141 agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat
     1201 ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg
     1261 cagggcggtg gcctcgtcac cagtggttca gacgcgacaa tcaatttttc tggcacggca
     1321 gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg
     1381 gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag tctggcgctg
     1441 ggtttgtggg cgctcagcgg cggtagaata accggagaca gtttggctat caccggcgcg
     1501 gcaggagcca gagggattta tgccatgacc aacagccaga tcgacctcac gagcgatctg
     1561 gtcattgata tgagtacacc cgaccagatg gccatcgcaa cgcaacatga cgatggttat
     1621 gccaccagcc gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt tctttccaaa
     1681 ggtgggctaa tcaatctgga tatgcaccct gggtcggttt ggacaggttc ctccctcagc
     1741 gataatgtca atggcgggaa actggacgtt gcaatgaata acagcgtctg gaacgtaaca
     1801 agtaattcta atctcgacac gctggcgctg agccattcaa ctgtcgattt tgccagccac
     1861 gggtcaactg ccggcacatt taccacatta aacgtagaga acctgagcgg taacagtacc
     1921 tttattatgc gtgctgatgt tgttggcgag ggtaatggcg ttaataatag aggggattta
     1981 ttgaatatca gcgggagtag tgctggtaat cacgtattgg ctatccgcaa ccagggcagc
     2041 gaggccacaa cgggaaatga agttctgaca gtggtaaaaa ccactgacgg cgcggcctcg
     2101 ttcagcgcgt cttctcaggt tgagttgggg ggatatctgt acgatgtgcg taaaaatggc
     2161 actaactggg agctttacgc ttccgggaca gttccggaac cgactcctaa tcctgaaccc
     2221 acaccagctc ccgctcagcc tcccatagtc aaccccgatc ctacgcctaa acccaccacg
     2281 accgcagatg ctggcggcaa ttatctcaat gtcggttact tattgaacta tgttgaaaac
     2341 cgtacgctga tgcaacggat gggtgacttg cgaaatcaga gtaaagacgg taatatctgg
     2401 ttgcgcagtt atgggggaag cctggactcc tttgccagtg gcaaactgag cggctttgac
     2461 atgggttaca gcggtatcca gtttggtggg gataaacgtc tctctgatgt aatgccgttg
     2521 tatgtcggtc tgtatattga ctcaacacat gcatcgccgg actatagcgg aggcgacggt
     2581 accgcacgtt cagactacat gggaatgtac gccagttaca tggcacaaaa cggtttttac
     2641 agcgatctcg ttataaaagc atcgcgccag aaaaatagtt tccacgtact ggacagtcag
     2701 aacaactgcg ttaacgccaa cggcactgcg aatggaatga gcatctccct ggaagccggg
     2761 cagaggttca acctgtcccc tactggttat gggttctata tagagccgca aacccagctt
     2821 acatacagcc accagaatga gatggctatg aaggcgagta atggcctcaa tatacatctg
     2881 aatcactacg aatcgctgct ggggcgtgcc agcatgatac tggggtatga catcaccgca
     2941 ggcaacagcc agctgaatgt ctatgtgaag actggcgcta tccgcgagtt ttcaggggat
     3001 atcgaatatc tgttgaacga ctcccgggag aagtacagtt tcaaaggtaa tggctggaat
     3061 aacggcgtgg gagtcagtgc acagtataac aaacagcaca cattctatct cgaagcggat
     3121 tacacgcagg gtaacctctt tgatcagaag caagtcaacg gaggatatcg cttcagcttt
     3181 taagtgcggg atagacaagc ccttaaacga tgctgaaaat tgttagcgtg cgccggaatg
     3241 aaatacagat cactctgatg tatcagaaat cgtttctgtc tttcataccc ggcgcttaaa
     3301 ctgccctgca tcaatgccat gttgcttcat tttccgccac agcgtagtgc ggccgatccc
     3361 aagtaaagcc gacatttcct gaatgcgacc gcctgtgacc tgggctgcgt taataattgc
     3421 ctctttttca acttccgcaa atgacagact ggtggaaagg cgggtggcgc tgacatcatc
     3481 tgtcgcctgc tcggtaaaca gatgttcagg taaatcactg acgcgaatgc gcccgttatc
     3541 actactcaga gcaagattct cgatgacgct gtaaagttca aaatcgttgc ctggccatgc
     3601 acaagaaact aggcgagcga gggcatcgtc atcaattttc agccgcgtag agaagcgttt
     3661 ttcaagactg cgtaatttgt tattcaccag cgccggaatg ctgccacgcc gcatacgcag
     3721 aggcgggatg gtaatttcaa atgcatgcag cgcgtaatac agctggcgac taaaacgatt
     3781 ttgttccacc agcattgcga ggtccgcggt cattgttgca atcactttga catcaattgg
     3841 tattaaacgc cgcgcatcca gtcgcgtgat aaccccctgc ttgataacct gcagcaaagc
     3901 agactgtaac tccaccgcca gatattcaat cttttcaaga aacagcgtgc cgccgtgtgc
     3961 cagttccagc cgactcagac ggccattttc attgtccgtg cgatcgccac caataaattc
     4021 ttccgccagc gcagcatcac catataactc acaattgacg gcgatataag gacctgcagc
     4081 acgctcgctt tcattatgaa ttgcctgact tagcagtgcc ttgcccacgc cctcttctcc
     4141 acaaagcagg acaggaaagc tactgcgcgc cgcctggcga ccaaaatgaa tcaagcggcg
     4201 ggtttgcgga tcgtcctgtg gcatatgagc gaaggtatgg ctgacttttc ctaattgact
     4261 ggtcattaac tgccgcatct gttccacagg atggagcaac aaaataaagc tggttccctg
     4321 cgtttctatt atcggtttaa gggttatcac cgcatcaata aactggtgct ggctttcaaa
     4381 ggttgcttct acgtgtttga gcggatgtgc ctgttttatt gcttgttgca atacggcggg
     4441 taacgttaag agttcagtga ttgcccgtcc ctgacttgcc gtcgcgtcaa ggcgcaagac
     4501 ccgcgccgcc tgggcattaa taaattgcaa attaccctgc tcgtcccagc taatcacgcc
     4561 atcatccata ctttctaaca gggcattaag ttgatttaaa tgacggttag tttcagcgag
     4621 caaactgtcc gtcagcagta aattaccgac ctcgcgggcg attgccaacg tcaacggcaa
     4681 atcagctgcg gtagtttgct caaccggaca cgccagcgct attgttcccg tcaatcggcc
     4741 cttgctgtca aacaacggcg ttgcacaaaa ggcccagttc cagagtgcct gtttgaaatg
     4801 ttgatcggcc atcgttttca cggcctgacc agagatagcc gctaacgata gcgcacaagt
     4861 accaataatt ccctcggcgc aatacgtgcc gtcattgaat cccagtgcac ttagctgctg
     4921 caaggtttgc ggatcgccat tacggctgag aatgcaggcg gtttcatcga ggataaacag
     4981 cgcacactct cgcggtgcca tatattccca ggcatcttcc agcgcagcct gcccgagcgt
     5041 cagcatcgct ttcttacgcc gataaataga agcaaatgtc acgccctggg cctgatgtgg
     5101 tacgttccat gtctcccgtt tcatcagctt attgcatcgc tcccaggagg ttgcaattaa
     5161 gggagatatg ccccgaccat cgttgttaaa agcgccactc atatcccgca tcccttatga
     5221 caaaaggtgt tccggctcgc aattttaagt attaatacac gtaaaagttt caatatggaa
     5281 cattcattaa agcatatgtt tcactatgaa acataatttg ggctaatttt cttttccctt
     5341 gcctgatgca caatggattc atcggcagtc agtggtcgcc gtgtcgttga acatcatcca
     5401 tgccctaccg taattgctgg cgcaaaataa tgaaaaaatt gatcaatgat gtgcaagacg
     5461 tactggacga acaactggca ggactggcga aagcgcatcc atcgctgaca ctgcatcagg
     5521 atccggtgta tgtcacccga gctgatgccc ctgttgcagg aaaagtcgcc ctgctgtcgg
     5581 gtggcggcag cggacacgag ccgatgcact gtggttatat cggtcagggg atgctttcgg
     5641 gggcctgtcc gggcgaaatt ttcacctcac cgacgcccga taaaatcttt gaatgctcca
     5701 tgcaaattga tggcggcgaa ggtgtactgt tgattatcaa aaattacacc ggcgatattc
     5761 ttaactttga aacagcgacc gagttactgc acgatagcgg cgtaaaagtg accactgtgg
     5821 tcattgatga cgacgttgca gtaaaagaca gtctttatac cgccgggcga cgcggcgttg
     5881 ccaacaccgt attaattgaa aaactcgtag gcgcagcggc ggagcgtggc gactcactgg
     5941 acgcctgtgc ggaactgggg cgtaagttga ataatcaagg ccactcaata ggtatcgctc
     6001 tcggtgcctg taccgttcct gccgcgggca aaccttcttt tacccttgcg gataatgaga
     6061 tggagtttgg cgtcggcatt catggtgagc caggtattga ccgccgccct ttctcttccc
     6121 ttgatcaaac cgtcgatgaa atgttcgaca ccctgctgga acatggctca taccatcgca
     6181 ctttgcgttt ctgggattat caacaaggca gctggcagga agaaccacaa accaaacaac
     6241 cgctccagtc tggcgatcgg gtgattgcac tggttaacaa tcttggcgca actccgcttt
     6301 ctgagctgta cggcgtctat aaccgcctga ccacacgttg ccagcaagcg ggattgacta
     6361 tcgaacgtaa tttaattggc gcgtactgca cctcactgga tatgaccggt ttctcaatca
     6421 ccttactgaa agttgatgac gaaacgctgg ctctctggga cgccccggtc cacaccccgg
     6481 cccttaactg gggtagataa ggagaaagca atgtcactga gcagaactca aattgttaac
     6541 tggctcactc gttgtggcga tattttcaac accgagagcg agtatcttac cgggctggat
     6601 cgcgagattg gcgacgctga ccacgggcta aatatgaacc gaggctttag caaagtggtg
     6661 gaaaaactcc ctgctatcgc agataaagat atcggtttca ttctcaaaaa taccggtatg
     6721 acgctgcttt ccagcgtcgg tggtgccagt ggtccgctgt tcggtacctt ctttatccgc
     6781 gccgcacagg cgacccaggc acggcaaagc ctgacactgg aagagcttta tcagatgttc
     6841 cgcgatggcg cggacggcgt aatcagtcgc gggaaagccg aacctggcga taaaaccatg
     6901 tgtgatgtgt gggtgccggt ggtggaatcg ttacgtcagt ccagcgagca aaatctctct
     6961 gttccggtgg cgctcgaagc tgccagtagc atcgccgaat ccgcggcaca aagtacgatt
     7021 acgatgcaag cccgcaaagg ccgcgccagt tatctcggtg aacgcagtat tggtcaccag
     7081 gatcccggcg cgacctcggt gatgtttatg atgcaaatgt tggcgttagc cgcaaaagag
     7141 taaggaattg gtgatggtaa acctggtcat agtttcacat agcagccgac tgggagaagg
     7201 tgtcggtgaa ttagcccgtc agatgttaat gagtgatagt tgtaaaatcg ccattgccgc
     7261 gggaattgac gatccacaaa atcccattgg taccgatgcc gtcaaagtga tggaggccat
     7321 cgaatctgtt gctgatgccg accatgtgct ggtcatgatg gatatgggta gcgcattatt
     7381 gagtgctgaa actgcgctgg aattgctggc tcccgagatc gccgcaaaag tacgtttgtg
     7441 tgctgcgccg ttggtcgaag gtacactggc agcaacggtc agcgcggcct cgggggcgga
     7501 tatcgacaaa gttatctttg acgctatgca tgcgctggaa gccaaacgtg aacaactggg
     7561 tttaccgtcc tccgacactg aaatctctga cacatgtcct ccgtacgatg aagaagcccg
     7621 ttctctggcg gtggtcataa aaaaccgtaa cggcctgcat gtacgtccgg cctcccggct
     7681 ggtttatacc ttatcgacat ttaatgccga tatgttgctg gaaaaaaacg gcaaatgcgt
     7741 cacaccagag agtattaacc agattgcgtt actacaagtt cgctataacg atacgctgcg
     7801 cctgattgcg aaagggccag aagctgaaga ggcactgatc gctttccgtc agctggctga
     7861 agataacttt ggtgaaacgg aggaagtcgc tccacctact ctgcgtcccg ttctgcctgt
     7921 ttcgggtaaa gccttttatt atcaaccagt tttatgtacg gtacaggcaa aatcaaccct
     7981 gaccgcggaa gaagaacaag atcgattacg ccaggctatt gacttcacgt tattagatct
     8041 gatgacgtta acagcgaaag cagaagccag cgggcttgac gatattgccg caatcttttc
     8101 tggtcaccat acactgttag gtgatccgga actgctggcg gcggcaagcg aactccttca
     8161 gcatgaacat tgcacggcag aatatgcctg gcagcaagtt cttaaagaac ttagccagca
     8221 ataccagcaa ctggatgatg aatatctaca agctcgctat attgatgtgg acgatcttct
     8281 gcatcgcacc ctggtccacc tgacccaaac gaaagaagaa
//
LOCUS       DADLZM010000157         8194 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLZM010000157 DADLZM010000000
VERSION     DADLZM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.159"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNY73_003537"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNY73_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAY8049404.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISVLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNY73_003538"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNY73_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY8049405.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="JNY73_003539"
     CDS             complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="JNY73_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAY8049406.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPDFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="JNY73_003540"
     CDS             complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="JNY73_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY8049407.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="JNY73_003541"
     CDS             complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="JNY73_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY8049408.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3681..4508
                     /gene="nadE"
                     /locus_tag="JNY73_003542"
     CDS             3681..4508
                     /gene="nadE"
                     /locus_tag="JNY73_003542"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY8049409.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4738..5625
                     /gene="cho"
                     /locus_tag="JNY73_003543"
     CDS             4738..5625
                     /gene="cho"
                     /locus_tag="JNY73_003543"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY8049410.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5585..6160)
                     /gene="ves"
                     /locus_tag="JNY73_003544"
     CDS             complement(5585..6160)
                     /gene="ves"
                     /locus_tag="JNY73_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY8049411.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLWLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6363..6848)
                     /gene="spy"
                     /locus_tag="JNY73_003545"
     CDS             complement(6363..6848)
                     /gene="spy"
                     /locus_tag="JNY73_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY8049412.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7178..8146)
                     /gene="astE"
                     /locus_tag="JNY73_003546"
     CDS             complement(7178..8146)
                     /gene="astE"
                     /locus_tag="JNY73_003546"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY8049413.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2153 a         2055 c         1890 g         2096 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtac gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcaatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagt
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgacccctcc ccaaagctta
     2281 gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc attaagcgaa
     2341 taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact gcaaaaggga
     2401 ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct taaaaaccgc
     2461 aatttaaata ttgcggtttt gatttatgaa ataactcttt gacgggaaaa tttaaaataa
     2521 attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg cctaaaccat
     2581 cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct
     2641 ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac
     2701 ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt
     2761 ctgcctgtgc gcgcattttt gataccagta aagagataga catgcccgca gaacaaaaca
     2821 gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca
     2881 ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt
     2941 tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata
     3001 aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg
     3061 aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact
     3121 caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag
     3181 gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct
     3241 gctttaccat cacgttgacc caggatgtag gtctggcaag taccgcgagc atggatcatg
     3301 ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt
     3361 ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata agctgtacaa
     3421 cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata
     3481 ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca
     3541 agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat
     3601 tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga agttttgtct
     3661 tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa
     3721 aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt cgattttctg aaaagctacc
     3781 tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca
     3841 cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgccaggaa actggcaacg
     3901 aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggctgac gaacaagatt
     3961 gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg
     4021 cggtattggc tagcgagcag gcattgcggg aagcaggcat tgaactgagc gattttgtcc
     4081 gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca
     4141 gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat
     4201 atggtgacgg cggtacggat attaatccgc tgtatcgtct caacaaacgc cagggtaaac
     4261 agttactgac ggcattaggt tgcccggaac acctttataa gaaagcccca actgccgatc
     4321 ttgaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata
     4381 tcgacgacta tctggaaggg aaaaacctac ctgaacaggt cgccagaaca atagagaact
     4441 ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa cgttttcgat gatttctgga
     4501 aaaagtaata atttgcacct attggattgt gcgaaaaaga gtaatttgtt cacgccggat
     4561 gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatacat tgcaggagtc
     4621 gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatca acccgcctct
     4681 tttttaatcg cctcccgcct gttacactgg atagataacc agcattcgga gtcaacagtg
     4741 gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc
     4801 gaacatttac attcattcct taatgactta cccacccgac caggggtgta tctgtttcat
     4861 ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg
     4921 ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc
     4981 tggatctgca ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa
     5041 caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta
     5101 aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac
     5161 ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagag catcgctgat
     5221 gaacaaaaac tttgttatgg cctgttggga ctggaaccgt taagtcgcgg tcgtgcatgt
     5281 tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa
     5341 catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa
     5401 ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac
     5461 tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc
     5521 gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat
     5581 gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca
     5641 gtttaccttg cggttgtaaa agccacaggg tgtgcctgcc atcgaaccag cacgcgccct
     5701 ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc gttaatcaca aacaccaccc
     5761 cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga
     5821 catccaggcg cgtcataata ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt
     5881 ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc
     5941 ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc
     6001 cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa
     6061 aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc
     6121 acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct
     6181 tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt
     6241 agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg
     6301 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa
     6361 gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt cagacgcttc
     6421 tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta aattttgttc
     6481 tgggtttcca tgtgcgccag catgttagct ttgcgctgtt cttccatttt tgcgatctgc
     6541 gcttcagctt ttgctttatc gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt
     6601 tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc gtggatctgc
     6661 tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg cggaccgaac
     6721 ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg
     6781 gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat
     6841 ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc
     6901 cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac
     6961 aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatgga
     7021 gagtgatgag agaatattac aacacgatga ttttgcagag attatgaaga actataccgg
     7081 atgactggtg ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc
     7141 aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt tcgagcatta
     7201 atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat
     7261 gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggca
     7321 taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg cgagtaattt
     7381 gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata
     7441 gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg
     7501 gcaacgcttt gccaagttcc aacgtacagg ccagcgcgcc aaaatgtctg acgctgaaat
     7561 gggtaaacgt accaccaggc tcctgatgga acaccagcgc ctccagcccc gccgcaccca
     7621 gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg
     7681 gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccaacgcaca gattctttgc
     7741 cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc
     7801 tttcagcaaa tagctgccaa cgaccgccaa acattcggtt catatcgcta tggcaataac
     7861 gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg
     7921 ggatctcgct gtgagatatc gcgccaagca acgcgtccag catttccaca ggtgccgtct
     7981 cattaccgtg gatccccgca gaaatcacca gcgcgccttg cggtggcgtt aatggcgtca
     8041 gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga
     8101 taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg
     8161 cgctggaacg gataaaccga accgagattc agta
//
LOCUS       DADLZM010000158         8165 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLZM010000158 DADLZM010000000
VERSION     DADLZM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.160"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..872)
                     /gene="mltC"
                     /locus_tag="JNY73_003547"
     CDS             complement(<1..872)
                     /gene="mltC"
                     /locus_tag="JNY73_003547"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY8049414.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSR"
     gene            complement(937..1212)
                     /locus_tag="JNY73_003548"
     CDS             complement(937..1212)
                     /locus_tag="JNY73_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY8049415.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(1240..2292)
                     /gene="mutY"
                     /locus_tag="JNY73_003549"
     CDS             complement(1240..2292)
                     /gene="mutY"
                     /locus_tag="JNY73_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY8049416.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            2453..3172
                     /gene="trmB"
                     /locus_tag="JNY73_003550"
     CDS             2453..3172
                     /gene="trmB"
                     /locus_tag="JNY73_003550"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY8049417.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3172..3498
                     /locus_tag="JNY73_003551"
     CDS             3172..3498
                     /locus_tag="JNY73_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY8049418.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            3682..4401
                     /locus_tag="JNY73_003552"
     CDS             3682..4401
                     /locus_tag="JNY73_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY8049419.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            4577..5623
                     /gene="ansB"
                     /locus_tag="JNY73_003553"
     CDS             4577..5623
                     /gene="ansB"
                     /locus_tag="JNY73_003553"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY8049420.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            5740..6747
                     /locus_tag="JNY73_003554"
     CDS             5740..6747
                     /locus_tag="JNY73_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY8049421.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(6803..8104)
                     /locus_tag="JNY73_003555"
     CDS             complement(6803..8104)
                     /locus_tag="JNY73_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY8049422.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKHRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTLPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         2223 a         1966 c         2087 g         1889 t
ORIGIN      
        1 cgcgatgttg ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg
       61 gtgccggtat caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat
      121 ttcccctgcg agcggaacac atctttcccg gcagtatttt gtaccacctg cattaatccc
      181 agcgcatcgg aacggctgac cgcatacggg ttaaaggaag attcagtctg cataattgcc
      241 agaatcagcg actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat
      301 ttgtgcgcac gtttatcaag gtggttcggc accatgttaa tggtgacgct gtagatgata
      361 cgtagcccgt tgctgcggct tttcagacgg tttttcagca gataatccgc gaagttgctt
      421 gcgcgacctt cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa
      481 ggttctttcg aaattgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg
      541 tcacccatca gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg
      601 gcgatggttt cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat
      661 tgatcggtgt atttcacgta gtccttagga ccggcgatca ccacctcttt gaagccccag
      721 atgttctcaa tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg
      781 acccaggctt cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc
      841 gcaatcaaag ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt
      901 tatgcaagct gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata
      961 gccctcgata tgcacctctt taccctcgaa caggaagttg accatctcct gctccagcag
     1021 cttgcggtgc tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgttt
     1081 gtgctgccac tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc
     1141 gccggggtac agctgaaaat cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat
     1201 cgttctgctc ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg
     1261 taactgctgt aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa
     1321 gttataccag agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca
     1381 cataggcaca atatctaagt ggaaatggct gaaggtatgg cgaaacgcgg tcagttgcgt
     1441 caggttatcg gcagcaatct gccgttgtgc cagccaatgc cgcaaacttt cttcgtcggc
     1501 aaactgcggg aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa
     1561 tacttcatct tcgtgctgta acaggagaaa gtagccggtg cgctccggca gcgtctgttt
     1621 cggttttttg cccggataaa gcgaccagct attgttggtg gcggcaatac atccgttttg
     1681 tagcggacag agcgaacatt tcggcttcga acgcgtgcaa atcatcgcgc ccaaatccat
     1741 catcgcctga ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca
     1801 tagtttattc tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag
     1861 cacgcgtttg acgttaccgt cgagaatcgg aaagtgcttg cccagagaaa gcgagagaat
     1921 cgcgcctgcg gtggaacgcc cgacgcccgg taacggcgcg acttcttcaa aggtttccgg
     1981 gaatttacca ccgtgtaagg tcgccacttg ttgtgccgct ttatgcaggt tgcgcgcgcg
     2041 ggcgtaatag ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag
     2101 atcagtcacc gtcgggaagt gcgccataaa gcgttcaaaa taggggataa cggtcgcaac
     2161 ctgagtttgt tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg
     2221 ccagggcagc gttttccgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg
     2281 cgacgcttgc atggtcaccg acttcactgt tgttgggggc aagattgcag cacagcgacg
     2341 gcagggtgta aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact
     2401 aactttggat aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa
     2461 cgacgtcatt tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt
     2521 tgtgcgccgc caggggcgac tgaccaaagg ccaggaacat gcgctggaaa attactggcc
     2581 ggtgatgggc gttgagttca gtgaagatat gctggatttc cccgcgcttt ttggccgtga
     2641 agcgccggtg acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa
     2701 agatcgcccc gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg
     2761 cctggcttct gcgcatgaag aaggtttaag caacctgcgc gtgatgtgtc acgatgcggt
     2821 tgaagtgctg cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc
     2881 tgacccgtgg cacaaagcgc gccataataa acgccgtatc gttcaggtgc cgtttgccga
     2941 actggtaaaa agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc
     3001 ttatgcggaa catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga
     3061 gagcaatgat tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg
     3121 tcatcgtctt ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag
     3181 aaccgtagcc gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt
     3241 tcggtggcat ggcgattccc ggaaggtaca tcggaagaac agattgataa aatcgttgat
     3301 gactttatta acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg
     3361 gcctgggaag gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg
     3421 attgtgcgta agtggctgga agagtgcaaa ctggatgagg tacgcaccag cgaacttttc
     3481 gacgtttggt gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg
     3541 ctacgcttat caggcctaca gagatgcacg atcgagtagg cgggataagg cgtttacgcc
     3601 gcatccggca tggaaaacgc gtactttgtt atcaatctgg ggccagcata tgctggcctg
     3661 atttgttctt gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg
     3721 acggcaatga ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt
     3781 gtcagcccgc aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac
     3841 ggcaacgtga tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg
     3901 aaggattatc aggctgaact acgtagcacg ctgccgtgga ttgatgaagg cgcgaaaagc
     3961 cgcgtcgaga aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgacagc
     4021 agcaaaatgc gcagccgtct gaccaaactt gatgcgcagc ttaaagagca gatgaatcgc
     4081 attatcgaaa cgcgcagcga tggcctgacg ttccactata aagccattga tcaggttcgc
     4141 gccgaaggcc agcaactagt gaatcaggca atgggcggaa ttttacagga cagcattaat
     4201 gaaatgggcg cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga
     4261 agcttgggcg gtctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc
     4321 cagcagtttg gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc
     4381 ctggtcggga atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt
     4441 ttaattcttc gttttgttac ctgcctctaa ctttgtagat ctccaaaata tattcacgtt
     4501 gtaaattgtt taacgtcaaa tttcccatac aaagctaagg gataatgcgt agcgttcacg
     4561 taactggagg aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg
     4621 ttttagtggt gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat
     4681 tgccggtggt ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga
     4741 aaatctggtt aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt
     4801 agtgaatatc ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat
     4861 taacgccgac tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga
     4921 agaaactgct tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg
     4981 cgcaatgcgc ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt
     5041 agtgaccgca gctgataaag cctccgctaa tcgtggcgtg ctggtggtga tgaacgacac
     5101 cgtactggac ggtcgcgatg tcaccaaaac caacaccacc gacgtagcga ccttcaagtc
     5161 tgttaactac ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc
     5221 ggcacgtaag cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa
     5281 agtcggcatc gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga
     5341 tgcgggctat gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt
     5401 gttcgacacc ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt
     5461 accgacgggt gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc
     5521 ctctggcacg ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac
     5581 caaagatccg cagcagatcc agcagatttt caatcagtac taatcacctc gtcccggcat
     5641 cgtgccgggg ctttttcact tcagacttac gtccattgcc aattttcatt atcctaatga
     5701 taatcaccgg aataaattat tccgcgcgag ggttttcggg tgaaaaagca atggattgtt
     5761 ggcacggcgc tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact
     5821 gaaaatatct taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgcc
     5881 atcagcttaa aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat
     5941 gtctcttcca gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc
     6001 gaacagacct ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga
     6061 acgcccgcgt ctggctggtc ggtgaacttt tactcttttc aggcggcagc cagcgatcgt
     6121 gggcgggtgg tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat
     6181 ttcaattatc gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct
     6241 gcgctggaaa aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg
     6301 gatgcatatt ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa
     6361 aaaattcacc aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt
     6421 aaatatgaca aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag
     6481 tgttatgaag tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag
     6541 acttttctgc aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa
     6601 aaagggcaat atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt
     6661 ttgatgaaaa ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa
     6721 ctgcggcgca atggggtgat taagtaactc atgttaagtg cctttgccgg ataatataaa
     6781 aagttatccg gcaaattatt ttttacatta gccctagtag ctgtggaatg aacaaggata
     6841 tttgcggaat gtaagtgatc atcatcaacg ctactagcaa tgcagcataa aatggcagca
     6901 atggtttgat taaatgttga atcttcacac ccgagataga acaaccaaca aatagcgcac
     6961 tacccaccgg cagtgttaac agcccaatac aaaggttggc caccatcata ataccgaaat
     7021 gtactgggtc cattcccagc tcttgtgcaa tcggcaagaa gatcggtgta aagattagta
     7081 cagccggtgt catgtccata aagatgccga caattaataa gacgatatta atcagcagca
     7141 atatgatcag tgggttatca gaaatcccca tcaatgcgtc gctgatcata taaggaatat
     7201 cagcattagt cattgcccag gacatgccca cggaaaaacc gatcaacagt aatacaatcg
     7261 aagtggtaac aaccgactcc agaatcagtt tgggtaaatg gcgccatttt acttcccggt
     7321 aaatcagcac cgagagaata aatgtgtaga caacggcaat tgctgacgct tcagttgcgg
     7381 taaaaatgcc gcccagaatg ccgcccatca caatgaacac cagaagcaaa cttggtaagg
     7441 catccagaaa cgctttacag gcctgagcaa aggttgctct ttctgaaagc gggtagccac
     7501 ggtgtttagc aataatgccg cagactatca ttaccgcaag acccattaag atccctggca
     7561 aatatcccgc cataaacagc gatgctacag aaacgccgcc agctgtcagg gaaaacacaa
     7621 ttaacacgtt tgagggcggt atcagtaatc cagtaataca agatgagacg tttacagcgg
     7681 tagaaaatgc cggatcataa ccttctttgg tctgaatggg attcagtgtg ccaccaacgg
     7741 cagcagctgc cgcaacggct gaaccagaaa tggaaccaaa catcatattc gccaggacgt
     7801 tcacatgacc aagcgaacca ggaacgcggc caaccattac ctgggcgagg ttaatcaaac
     7861 gtatcgcaat accgccgctg ttcatcagtg ttccggcaaa gatgaaaaag gggattgcca
     7921 acagggcaaa gttatccagt ccattagcca gtcgttgcga aacagtgatt gccgcgatat
     7981 cccagggaaa catcaaaagc attgaagcta cagtggcaat accgattgag aacgaaatgg
     8041 gcacaccaat aaagaccagc acaaaaaaac agccaaacag tgtaatcgcg atataactat
     8101 ccattaactg gcctcctgcg caggttgttt cagttgttga atgctttgat taataaaaca
     8161 caagg
//
LOCUS       DADLZM010000159         8079 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLZM010000159 DADLZM010000000
VERSION     DADLZM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.161"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1242)
                     /locus_tag="JNY73_003556"
     CDS             complement(<1..1242)
                     /locus_tag="JNY73_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY8049423.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="JNY73_003557"
     CDS             complement(1239..2117)
                     /locus_tag="JNY73_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8049424.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="JNY73_003558"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="JNY73_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="JNY73_003559"
     CDS             complement(3022..3255)
                     /locus_tag="JNY73_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049425.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="JNY73_003560"
     CDS             complement(3252..4109)
                     /locus_tag="JNY73_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY8049426.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="JNY73_003561"
     CDS             4134..4463
                     /locus_tag="JNY73_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY8049427.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="JNY73_003562"
     CDS             4453..4761
                     /locus_tag="JNY73_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8049428.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="JNY73_003563"
     CDS             complement(4803..4979)
                     /locus_tag="JNY73_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY8049429.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="JNY73_003564"
     CDS             complement(4976..5386)
                     /locus_tag="JNY73_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY8049430.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="JNY73_003565"
     CDS             complement(5383..5661)
                     /locus_tag="JNY73_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049431.1"
                     /translation="MNDSYRQFENWWSKDESQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..>8079)
                     /locus_tag="JNY73_003566"
     CDS             complement(5736..>8079)
                     /locus_tag="JNY73_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HAY8049432.1"
                     /translation="GDCEPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPF
                     GGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1919 a         2203 c         1854 g         2103 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcatcgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcgga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctgcc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gctttcgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct
     6061 tttacgtcca ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg
     6121 agaatgacgt gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc
     6181 tgcccgttat agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac
     6241 tggatcccgt atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc
     6301 gttccagtca ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt
     6361 tccagcactg gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata
     6421 ccaggcttca gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt
     6481 gccatatgcc cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg
     6541 tggcgagggt gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc
     6601 ctgacgaata ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt
     6661 gaacggatgg cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc
     6721 ccatgccgct tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat
     6781 gacccgccga gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag
     6841 gaaaaaacaa gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc
     6901 atgcctatac gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt
     6961 gctgtgcacc ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca
     7021 cgcgccgttg atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca
     7081 gccttcatac gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta
     7141 agactaccat ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca
     7201 aagtcaccag ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac
     7261 cgcttcaacc cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta
     7321 gtgacctccc ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact
     7381 cggtaaacat ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct
     7441 actgacgcca cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct
     7501 aaaagaatag gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc
     7561 gtcaactctg cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac
     7621 agatactgct gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg
     7681 tagagttctt caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg
     7741 gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa
     7801 cgatgttcac cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg
     7861 tccatcgaga tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac
     7921 tgttgcttag cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc
     7981 gcatagctca tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc
     8041 agagcctgcc atccgaacag gcaaggttcg caatcacct
//
LOCUS       DADLZM010000160         7875 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.162, whole genome shotgun sequence.
ACCESSION   DADLZM010000160 DADLZM010000000
VERSION     DADLZM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.162"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            163..648
                     /locus_tag="JNY73_003567"
     CDS             163..648
                     /locus_tag="JNY73_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049433.1"
                     /translation="MRVIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSADLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELRERYLKNATITLNVIMPETKFTLLNVT
                     K"
     gene            645..1031
                     /locus_tag="JNY73_003568"
     CDS             645..1031
                     /locus_tag="JNY73_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049434.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGDYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LACWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3268
                     /gene="tssI"
                     /locus_tag="JNY73_003569"
                     /pseudo
     CDS             1056..3268
                     /gene="tssI"
                     /locus_tag="JNY73_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            3291..3735
                     /locus_tag="JNY73_003570"
                     /pseudo
     CDS             3291..3735
                     /locus_tag="JNY73_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3758..4165
                     /locus_tag="JNY73_003571"
     CDS             3758..4165
                     /locus_tag="JNY73_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY8049435.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4162..6640
                     /locus_tag="JNY73_003572"
                     /pseudo
     CDS             4162..6640
                     /locus_tag="JNY73_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            6637..7495
                     /locus_tag="JNY73_003573"
                     /pseudo
     CDS             6637..7495
                     /locus_tag="JNY73_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            7595..7798
                     /locus_tag="JNY73_003574"
     CDS             7595..7798
                     /locus_tag="JNY73_003574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049436.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
BASE COUNT         2013 a         1821 c         2298 g         1743 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagt gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg acctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct tcgggaacga taccttaaaa acgcaactat aacgttgaat
      601 gtcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga ttattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctggcatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggcagcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcggc ggcgtcgctt gcagccggtg gtccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gggccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgcag gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg gcacatgggg ccggtcatat ccgtaccctc atgaccgggt acacctttac
     2281 gttgatgaat cacccgacgg cagaaattaa tcaggaatat ctgctggtac agaccactct
     2341 gtttttacgg gataatgcgc agcacagcgg tcaggatcag cattttacct atgtcaccac
     2401 gtttgaactg ccccccacca gcgaagttta ccgcccacaa cgaaccctga gcaaacccca
     2461 caccaaaggc ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa tctggacgga
     2521 taaatatggc cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga atgacgaaaa
     2581 cagttcctgc tggatccgcg tcagctatcc gtgggcgggt aaaggattcg gcatgatcca
     2641 gatcccgcgt attggccagg aagtactggt ggatttcaaa aacggcgatc cggatttgcc
     2701 catcatcgtg gggcgcacct ataaccagga caccatgccg ccgtgggggc tgccgggcat
     2761 ggcgtcgcag agtgggatat tcagccactc attgcaggga gggccgacta acggaaatat
     2821 gctgcgcttc gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg aaaaagacct
     2881 caataccacg gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca taaaaaccat
     2941 tgtcaaaaat gaaaccatca gcgtgggaga agaccgtaat gccaccatca caaaaaatga
     3001 tggtctttcc gtcaaactgg ctcaaaccat caatgtcgga acgacatatc gtctggatgt
     3061 tggcgatcaa tttacccttc gttgcggcaa tgcagcgctt gtattacaca aggatggctc
     3121 gattgagttt tgcggaaaac aactgttact gcacaccagc gatgttatgc aactcattgg
     3181 cagtggcatt gatatgaatc cggatggcgg cacagccatg actgcggatg atattgcccc
     3241 gttacctacc cccgacgttt ctgaataaaa aatataaaag gaatttttct atgaccagcc
     3301 tttaccgtat tcaggagggc tgctttgccc tgcctgaaac ttttcttgat cgcaccgtca
     3361 atatcttcgt cccatcgggc aatgaaaggg caactcccag tctgaatatt tcccgcgata
     3421 cgctcaggcc agatgaaagt ctgaccacct atattgaccg ccagatagcc ttgatgaaaa
     3481 aaaaacctgg gccagcaccg ggtactttcg cggggaacgg cactggcggg aactggcgaa
     3541 gctgccatga caggtgaaca gattctcgca actcatcgtt ctggaaaaac agaagtgtac
     3601 cagcgtcagg ccgggtttat caccgggcca gcaaaggtac taatgttgac gctgaccact
     3661 cagcgtcctt ttgatgatca caccgaccag ctatggactg catggttgac tagttttcag
     3721 cctgctaaaa gctaaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga
     3781 tgaccccatc taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat
     3841 cgccattatt gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat
     3901 tttgagtttt ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg
     3961 gcgctcgata cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa
     4021 cagtcgcccg gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga
     4081 aatccgcatt gcccagggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat
     4141 gaccacactg agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc
     4201 ggtacgcaga cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg
     4261 gatgtgctgt atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg
     4321 gcaaagatgg ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg
     4381 gtcgggatgg ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc
     4441 actgggcaaa aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc
     4501 acctgttcgc gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg
     4561 cggctgaact gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta
     4621 cagggtcggg aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac
     4681 ccggaagagc agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc
     4741 gaccgcagct attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt
     4801 atggagacgc cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg
     4861 gtggcctcca gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa
     4921 cgcctgtcgc atattgaact tgtgaagggc gggcgtcccg gtaacctggt ggaatatcgt
     4981 tacgacgata acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag
     5041 tttgcctatg aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc
     5101 taccactggg aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc
     5161 gaggattacc ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg
     5221 gagcagaagt ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg
     5281 ggcggcggaa tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc
     5341 cccggcgaac gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg
     5401 gacccggcag gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc
     5461 cgggcgctgg atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata
     5521 cgggagacgg acccggaagg gcgcgtcacg cggtacgggt atgatgaaca ggggctggcg
     5581 gtcagccgca cggatgcgcg gggcggcgag gttgtgctgg cgcacgatgc gcgcggtcaa
     5641 ctgaggcgtt acaccgactg ttcagggtgc gccacgggtt atgaatacga tgaagacggc
     5701 aacctgacgg cggtgacgga cgcggagggg aaaacggtac gcatcagcta taaccgtcag
     5761 gggctgccgg agacggtctg ccaccccggt aaacagcagg accgttacac ctggaacgcg
     5821 ctggggttac tgagcagcca ccgtcgcatc acgggcagtg tgcagtcctg gcagtatacg
     5881 ccgcgcggga tgctggcgcg ccatacggat gaagagaaac gggagacgcg ctggcagtac
     5941 accccggaag ggctggttgc cagtctgagc aacggcaacg gggctcagta ccggttcagc
     6001 tacgacggcg acggacggct gacgggagaa cagcgcccgg acggcctgat aaggatgttt
     6061 gcgctgaatg cggacgggtt tccggtcatc atccagacgc agggcacaga gggcggggtg
     6121 cgcaatgaac agcaggagcg tgatgcgctg ggccgcctgc tgcgcagtga cacgcagcac
     6181 agtacacgga ccttcagcta caaccgtctt gaccagataa cggaagtgac gctcaccccc
     6241 acggaagaag gggaacggct gcaccacatg caggcggaca cggtgcgttt tgcgtatgac
     6301 cgcagcgggt ggctgacggc agagcacagc gtgcacggca gtatcaaata ccggcgcgat
     6361 gcgctgggca atcccaccga tatcacgttg ccggacgggc agcatctcag tcatctttac
     6421 tacggcagcg ggcatctgct ccagacggca ctggacggta tcacggtcag tgaatatgag
     6481 cgtgacagcc tgcaccgtca ggtgatacgc acgcagggga aactggcgac gttcagcggc
     6541 tacaacgccg acaaccggtt aagctggcag cgcagcctgc caggtggcag ccagaatcca
     6601 cagcaggccg gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg
     6661 aacgcagacg gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc
     6721 tatgaccgca gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat
     6781 tattacgaca aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac
     6841 cgcctgccgg gctacgggcg tgaccgttac cgctataacg aatggggcga actgacagag
     6901 cgcagggacc agcaactgga gtggaacgcg cagggccagt taacgcgggt catcagcagc
     6961 aacagcgata cgctgtatca gtatgatgcg ctgggcaggc gcatcagcaa ggtgacatat
     7021 ggccgccatc acggggcgcg gagagagcca gaaaatctgg tattaccaca cggacctgac
     7081 gggcacggta cacgaggtaa ccgcgccgga cgggacgctg gtgtgggcgg gctaccaggc
     7141 gggatttggt gaaaaccggg gcgacatcag caacagcggg gcgtactttg agcagccgca
     7201 gcggctgccg gggcagtact ttgacgaaga aacagggctg cattacaatc tgttcaggta
     7261 ttatgcgccg gagtgcggga ggttcgtaag ccaggacccg ataggattaa acggtgggtt
     7321 gaacttatat gcttatgcgc ctaatccgtt aggttggatc gatccgctag gactgaattc
     7381 tttaggaata gattcaaata atatatttag aggagatggt aactataaag gtggcggaat
     7441 tggacagcca aagagtggca caatatcagc acaggacatt attgatcatc tctaagaaaa
     7501 aggtgatggg cgtttaacat cttttagtac taaaaaaaca tctagttcca actctcgtgg
     7561 tgcagatttt ttctcaaaaa accatgtctt caaagtggca gtttctgatc tcgatgattt
     7621 agttaaaaat aatcagatca ctgttttaac acaagaaaaa gtaaaagcaa tatttggttt
     7681 agaatcaagc aagaaaatta gaaaagaact tggtaatatt cttcagaata tggctaggaa
     7741 taatgaggtg ttagttcatg ggcaaattct tggaaataaa gttagccgat gtaattagtt
     7801 agtggagagg aagttgtgga cgaaattaga ataagaattg ctggtaatac ggctgcgcct
     7861 tgtttccatg tgcta
//
LOCUS       DADLZM010000161         7819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLZM010000161 DADLZM010000000
VERSION     DADLZM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.163"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            211..429
                     /locus_tag="JNY73_003575"
     CDS             211..429
                     /locus_tag="JNY73_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY8049437.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            647..889
                     /locus_tag="JNY73_003576"
     CDS             647..889
                     /locus_tag="JNY73_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049438.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVLGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="JNY73_003577"
     CDS             complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="JNY73_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY8049439.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="JNY73_003578"
     CDS             complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="JNY73_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY8049440.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2777..3679)
                     /gene="fdxH"
                     /locus_tag="JNY73_003579"
     CDS             complement(2777..3679)
                     /gene="fdxH"
                     /locus_tag="JNY73_003579"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY8049441.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="JNY73_003580"
     CDS             complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="JNY73_003580"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6155..6157),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY8049442.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6936..7769
                     /gene="fdhD"
                     /locus_tag="JNY73_003581"
     CDS             6936..7769
                     /gene="fdhD"
                     /locus_tag="JNY73_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY8049443.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1814 a         2013 c         2076 g         1916 t
ORIGIN      
        1 aatataatat attcatagca cccataactc atcctgggcg ataactaaac catttttgag
       61 taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg caaaaacaaa
      121 atgacatgca aaaatccgca tttttttaaa tgctttttcg gcgtaatttt cgcacataca
      181 ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta
      241 tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct
      301 gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc ggtgcgtaaa
      361 gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat
      421 aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat
      481 taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc gtatctggaa
      541 aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcggcaagg
      601 gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga actctctggc
      661 aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta agcaacttga
      721 tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag cggtagatca
      781 atacctgata aatcaatcgc aaacagcaag aaccagtgtt cttggcatct ggcaagggtg
      841 tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat gggaaaaaat
      901 gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg cgtgaacgcc
      961 ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt aggcctgata
     1021 agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata accttatcga
     1081 tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga atgggtaacg
     1141 cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg acctgcgcca
     1201 cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt taatggaact
     1261 acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga ggtcatctgc
     1321 cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc
     1381 atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt
     1441 gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg tttcacacag
     1501 gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg
     1561 catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc gggctttgcc
     1621 ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga taaacggcgc
     1681 tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca tatcttccag
     1741 ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc cgctcatttc
     1801 aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat cacgcggcag
     1861 aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc gcgcggtcag
     1921 atccatctcc agcggatggt cgtacagcac cacttcctgg gcgtgggcga taagcgcagc
     1981 aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac gctcggcgcg
     2041 gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat tcgccgtacg
     2101 tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc agctttcttt
     2161 tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg
     2221 gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc ggcgtagata
     2281 tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat taacgcgaat
     2341 cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat cacgccgctc
     2401 accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg accgaagtta
     2461 taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt cttcgcccaa
     2521 aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat gatgatgaac
     2581 gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg cggtgtgccc
     2641 atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct caccgccgcc
     2701 aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg
     2761 atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt
     2821 cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc gcaaaggtag
     2881 ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc acggtttcgc
     2941 tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca tggtgcagca
     3001 cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac
     3061 cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac
     3121 cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc ccaaccacca
     3181 cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg aggcgcggaa
     3241 tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg gactggaagt
     3301 cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct ttcaggcagc
     3361 ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt ttgtcgttct
     3421 gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca ttggggttgt
     3481 cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct gaacacgcca
     3541 cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc acttcttcct
     3601 ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga cgaatgatat
     3661 cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc acaaggaagg
     3721 acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa gtattggcaa
     3781 taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg atggtatcga
     3841 tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct ttggctttga
     3901 tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca agtttattcg
     3961 ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc aacagcgcgt
     4021 gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca tacgggaact
     4081 tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagcc gggttcgaga
     4141 taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg tagtgttccg
     4201 ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct tcctgctgca
     4261 tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga atatcccagc
     4321 cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac gggttaccct
     4381 gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat gcccatgccc
     4441 agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc atctggttgc
     4501 cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc
     4561 gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca ccggttgccg
     4621 gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct tccgacttcg
     4681 gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg ttcgcgccac
     4741 cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc tcaccgtcag
     4801 tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag cgacctgagt
     4861 taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac acttcggtct
     4921 ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag ttagaggttt
     4981 cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa ccgatcactt
     5041 tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag ccattgacct
     5101 tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc cacttcggca
     5161 accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag gccttcatca
     5221 tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc agcagcggtt
     5281 ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt ggcagcgtca
     5341 tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga atattggagt
     5401 gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc agcagctgga
     5461 tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag ccgagggcat
     5521 acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg cagactttca
     5581 ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg tagcgggaaa
     5641 cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg
     5701 cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg cgtttttccg
     5761 cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg atcaggctgg
     5821 cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc aggtacagca
     5881 atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag tagtcagcta
     5941 ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt
     6001 tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta ccgcccatca
     6061 ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa
     6121 atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg
     6181 cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg ttactcgacg
     6241 cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct tcggcgtttt
     6301 gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct
     6361 cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc ggaaacttca
     6421 gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa agtgcaccgc
     6481 ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg gcgttttttg
     6541 ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag gtgcaggtat
     6601 tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt gctacgctgg
     6661 gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag atcttaaaga
     6721 actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac gtcccattta
     6781 cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa ggggccgcaa
     6841 aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag aataccataa
     6901 tgttggtgtg tgtgttctta tctggttaag agaaagtgaa aaaaacacag cgaaaagaaa
     6961 tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg cgtgatgatt
     7021 tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg
     7081 gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt
     7141 tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc
     7201 cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt atggggttga
     7261 aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc gtggagcaac
     7321 ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt gatctcaaca
     7381 aactggatga tgcattacgt catctcaacg attttcagcc agtggggcaa ctgactggtt
     7441 gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg catgaagacg
     7501 tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa ggggaaagct
     7561 ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg caaaagtcgg
     7621 cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt gctgtagaag
     7681 tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg gcaacggttt
     7741 atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat atattaaatt
     7801 gcaggactta ttcatttcg
//
LOCUS       DADLZM010000162         7502 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLZM010000162 DADLZM010000000
VERSION     DADLZM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.164"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1677
                     /gene="cpdB"
                     /locus_tag="JNY73_003582"
     CDS             <1..1677
                     /gene="cpdB"
                     /locus_tag="JNY73_003582"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY8049444.1"
                     /translation="KGLKAGDVHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGA
                     KFPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVVPPQIMGWDKAN
                     LSGKVTMNDITETVRKYVPEMREKGADVIVVLAHSGLSADPYKVMAENSVYYLSEIPG
                     VNAIMFGHAHAVFPGKDFADIEGADITKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSG
                     KWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYS
                     YLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPASYVE
                     VEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIAPNSTKPQSLINW
                     DGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINVNAERIKNLTFNGKPIDPNAMFLV
                     ATNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAP
                     IAADKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(1803..2183)
                     /locus_tag="JNY73_003583"
     CDS             complement(1803..2183)
                     /locus_tag="JNY73_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8049445.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            2272..3132
                     /gene="qorB"
                     /locus_tag="JNY73_003584"
     CDS             2272..3132
                     /gene="qorB"
                     /locus_tag="JNY73_003584"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY8049446.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIARPTTTLAE
                     SVSHLFNVNN"
     gene            3241..4206
                     /locus_tag="JNY73_003585"
     CDS             3241..4206
                     /locus_tag="JNY73_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8049447.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLSQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            4314..4976
                     /gene="ytfE"
                     /locus_tag="JNY73_003586"
     CDS             4314..4976
                     /gene="ytfE"
                     /locus_tag="JNY73_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY8049448.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5021..6433)
                     /gene="cycA"
                     /locus_tag="JNY73_003587"
                     /gene_synonym="dagA"
     CDS             complement(5021..6433)
                     /gene="cycA"
                     /locus_tag="JNY73_003587"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY8049449.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(6741..7361)
                     /locus_tag="JNY73_003588"
     CDS             complement(6741..7361)
                     /locus_tag="JNY73_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8049450.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         1932 a         1968 c         1910 g         1692 t
ORIGIN      
        1 aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac gctggattat
       61 acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct gaaaaatgcg
      121 ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag aaccaaacag
      181 ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga cggaaaaaaa
      241 cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg ctgggataaa
      301 gctaatttat ccggaaaagt gacgatgaat gatattaccg aaaccgtgcg caaatacgtg
      361 cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc cgggctgtct
      421 gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga aattccgggc
      481 gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga ttttgctgat
      541 atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt aatgccgggc
      601 atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag cggtaaatgg
      661 caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa taaaaaatcc
      721 ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga tgccacacgc
      781 cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta tctggcgctg
      841 gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta tgtcgaacat
      901 tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc cgcaccgttt
      961 aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa aggccagttg
     1021 accttccgta atgccgccga tctttatctc taccccaata cgctgattgt agtgaaagcc
     1081 agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa ccagattgct
     1141 cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac ttataacttt
     1201 gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg ctatgacggc
     1261 gagtgccaga tgattaatgt caatgcagag cggattaaga acctgacctt taatggcaag
     1321 ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc ttacggcggc
     1381 aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga gaaccgctcg
     1441 gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca tccggcggca
     1501 gataacaact ggcgtttagc accgatagct gccgataaga aactggatat ccgtttcgaa
     1561 acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta tccgatgaat
     1621 aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag taagtaaaac
     1681 acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat gcgatgctgc
     1741 cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag cgcatccggc
     1801 agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag
     1861 tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag gctatattcc
     1921 acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg ttctaacgcc
     1981 tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg caggtcgcta
     2041 aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg gctggtgacg
     2101 tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc tttcagttgt
     2161 tgtgacaggc taacctgact catttcatac ttaccttttt gcatgtactt actaaaagta
     2221 agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc catgatcgct
     2281 attactggtg ccactggcca acttggtcac tatattattg aatccttgat gaaaacggtt
     2341 cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc cctggcagca
     2401 caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac atctgcactt
     2461 cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg tgccccgcag
     2521 catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc ttataccagc
     2581 ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga gacggagaaa
     2641 atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta cagcgaaaac
     2701 tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc ggcgggcgat
     2761 ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcact cgtgattagc
     2821 gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca
     2881 cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca aaatctgagc
     2941 gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact ggcggatatg
     3001 ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag caaaacgctt
     3061 agcaaattga ttgcccgccc aacgacaacg ttagccgaaa gcgtaagcca tctttttaat
     3121 gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa tgacgggatg
     3181 ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg gatgccagta
     3241 atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct tatttttgtc
     3301 gggccgttga tcgtgccgga atacccggcg atgttacagt cgatggggcg ttatctggcg
     3361 ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg tcagttggcg
     3421 cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat ttattacttc
     3481 tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat tatcggcacc
     3541 ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga cggcaaactc
     3601 gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc gagtgtgaat
     3661 attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac ctctggcatc
     3721 gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa cgcccgctgg
     3781 ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggcgcaggc gctggtcacg
     3841 ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat acaaacgccg
     3901 gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat ggttgcgata
     3961 gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca gcgattaccg
     4021 acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct gtacaccttt
     4081 ttactcagcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct gttagtgatt
     4141 ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc tgtctcagaa
     4201 agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta tagcttttgc
     4261 cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca gctatggctt
     4321 atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg gctctgtttc
     4381 gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc gcggcggcac
     4441 gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct gaacaaccga
     4501 ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc atcgtgcgct
     4561 accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact aaagtcgagc
     4621 gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg accatgctgc
     4681 atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg atgatcaaac
     4741 aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag cacgatgaag
     4801 cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg ccgccagaag
     4861 cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat gacctgatgg
     4921 atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt gagtgatcta
     4981 aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc agttcagcag
     5041 cccgcttctt accaataaac agccagccca accccagcgc gataaaccac agcggggtaa
     5101 ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg aacacaaaga
     5161 acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc gacttttcat
     5221 gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc gtccagacga
     5281 acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc acgctaggat
     5341 tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc aaccctttcg
     5401 cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct tcctgcgcca
     5461 gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac gccgcagagg
     5521 tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac acaaacagct
     5581 caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac ataatcacaa
     5641 tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc gcgcgtggca
     5701 gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca atccccacga
     5761 aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc gggaaccagc
     5821 cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac tgaaagtgca
     5881 tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt ttgatcatcg
     5941 caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc gtcagcagca
     6001 gcactatcac cgccagcgag gcgacccagt cggagaaatc ggggaaccag aactgagcat
     6061 aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac cagtaagtcc
     6121 agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca ctaaaagatt
     6181 tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa aagagcataa
     6241 aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc gttttgccgg
     6301 accccataaa caacccagta ccaatggcac cgccaatggc aataagctga atatgtcggt
     6361 ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca acgactttta
     6421 cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct ctacggcctt
     6481 tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg taacaatacg
     6541 cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg agtgtcatat
     6601 cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt tgcaacacaa
     6661 aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc ctctggttcg
     6721 aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa caccagggtg
     6781 ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc ctgcgggata
     6841 gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca gccagggatc
     6901 acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc aaacacggtg
     6961 ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc cggaattgcg
     7021 ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt cacaccttct
     7081 tttttggcgt tttcttccag gtatttcaca ccttcagcag ccatcgcctg gaaacgctga
     7141 cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac cacatcaacc
     7201 ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag tgcttctggc
     7261 agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc aatgccgtag
     7321 cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc tttcttaggg
     7381 tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt gtcgcaggaa
     7441 tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat ataataaagg
     7501 cg
//
LOCUS       DADLZM010000163         7462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLZM010000163 DADLZM010000000
VERSION     DADLZM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.165"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            215..291
                     /locus_tag="JNY73_003589"
     tRNA            215..291
                     /locus_tag="JNY73_003589"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /locus_tag="JNY73_003590"
     CDS             complement(337..1098)
                     /locus_tag="JNY73_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8049451.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="JNY73_003591"
     CDS             complement(1281..2171)
                     /locus_tag="JNY73_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY8049452.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNY73_003592"
     CDS             complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNY73_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAY8049453.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKDPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5131..7373)
                     /gene="nrfB"
                     /locus_tag="JNY73_003593"
                     /pseudo
     CDS             complement(5131..7373)
                     /gene="nrfB"
                     /locus_tag="JNY73_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
BASE COUNT         1738 a         1939 c         1952 g         1833 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatcgttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggatct
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag cgcggcgcgc
     3241 aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc ccgcgcaacg
     3301 taagcgttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag tgcgagttct
     3361 ggctgagctg ggatataacg ttgcgcatgc agccaccagt attgagcatt atttcccagc
     3421 tcacgttgtt ctgcctgttg aagccagcga tcgcgagccg cgccatttcc cgctgcctga
     3481 gcggtgttgg cggcggcgag cagatcctca ttgctcatgt cgtgaagact gattttctgc
     3541 caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc taccgcacga
     3601 tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg
     3661 ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc gtaggaaggc
     3721 gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc cggcaactga
     3781 ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc caccttcgcc
     3841 ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat taaagtctgg
     3901 ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc agcttcgcgt
     3961 gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag atttgtcggt
     4021 tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg ggtcgccacg
     4081 ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag cagtttttgc
     4141 gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac aacatactgg
     4201 cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt ggcgctgtat
     4261 ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag cggcttattt
     4321 tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa
     4381 gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg
     4441 tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc gcttaatgtg
     4501 ttctgctggc gtgcttcatt gtatagcgta tctgcctggg accattgttt caggtagatt
     4561 gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca
     4621 tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt ctgcccgact
     4681 tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc aagcagttct
     4741 tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg ctcaagtcgg
     4801 gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga
     4861 ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc cggcacctgt
     4921 tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt ctgtgccttc
     4981 agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag ccccagctct
     5041 tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt
     5101 aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg actccagttg
     5161 cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg aaacttgtaa
     5221 ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac
     5281 caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca cgttaattgc
     5341 tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact gatgcggcac
     5401 atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct gaaccgcatt
     5461 gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt
     5521 gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt tacgcgtcag
     5581 ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact catcattttc
     5641 cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt cggcaatcag
     5701 cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt gctctgcctg
     5761 cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac cgcccaggcg
     5821 tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga tgacctgatt
     5881 ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga cgctggggaa
     5941 gtcatgcgtt gttttatccc acgccacgcc acgcgacgcg gatcgccgtg ctgaagaacc
     6001 tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg
     6061 acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg
     6121 atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg
     6181 ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc
     6241 aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg
     6301 cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag
     6361 ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta
     6421 tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta
     6481 cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata
     6541 cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa
     6601 gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg
     6661 acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
     6721 agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac
     6781 ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa cagacgcaat
     6841 tccatcggtg aaatcacatc ttcggcgtca tgcagaataa aaccagcaaa agcgaaattg
     6901 gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcagacagtc ggctttgctg
     6961 gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc gcacacttcg
     7021 tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat atgatagttt
     7081 tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc cgatgacgcc cgtttcattc
     7141 cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc gcggtaactc
     7201 attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg
     7261 acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa cgttatcgcg
     7321 attactttta agctgtagag ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa
     7381 ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt atgaattgtg
     7441 acagcttttt accattaata gg
//
LOCUS       DADLZM010000164         7401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLZM010000164 DADLZM010000000
VERSION     DADLZM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.166"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..233)
                     /locus_tag="JNY73_003594"
     CDS             complement(<1..233)
                     /locus_tag="JNY73_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049454.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="JNY73_003595"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="JNY73_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY8049455.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="JNY73_003596"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="JNY73_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY8049456.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="JNY73_003597"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="JNY73_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY8049457.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="JNY73_003598"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="JNY73_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="JNY73_003599"
     CDS             complement(3305..3424)
                     /locus_tag="JNY73_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY8049458.1"
                     /translation="MPLSYAGYHHFIMMSLVSAEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="JNY73_003600"
     CDS             complement(3385..3570)
                     /locus_tag="JNY73_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049459.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="JNY73_003601"
     CDS             3671..3844
                     /locus_tag="JNY73_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049460.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3906..4190
                     /locus_tag="JNY73_003602"
     CDS             3906..4190
                     /locus_tag="JNY73_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY8049461.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="JNY73_003603"
     CDS             4194..4529
                     /locus_tag="JNY73_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY8049462.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="JNY73_003604"
     CDS             complement(4586..6907)
                     /locus_tag="JNY73_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8049463.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTETTNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="JNY73_003605"
     CDS             complement(7024..7233)
                     /locus_tag="JNY73_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049464.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2032 a         1742 c         1647 g         1980 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtatggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcagc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg ggggtaggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgttt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac c
//
LOCUS       DADLZM010000165         7297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLZM010000165 DADLZM010000000
VERSION     DADLZM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.167"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="JNY73_003606"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="JNY73_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY8049465.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAVVGKLNRVNQE"
     gene            1158..2123
                     /gene="rbsC"
                     /locus_tag="JNY73_003607"
     CDS             1158..2123
                     /gene="rbsC"
                     /locus_tag="JNY73_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY8049466.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2148..3025
                     /gene="rbsB"
                     /locus_tag="JNY73_003608"
                     /pseudo
     CDS             2148..3025
                     /gene="rbsB"
                     /locus_tag="JNY73_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3151..4080
                     /gene="rbsK"
                     /locus_tag="JNY73_003609"
     CDS             3151..4080
                     /gene="rbsK"
                     /locus_tag="JNY73_003609"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8049467.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4084..5076
                     /gene="rbsR"
                     /locus_tag="JNY73_003610"
     CDS             4084..5076
                     /gene="rbsR"
                     /locus_tag="JNY73_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY8049468.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5042..6469)
                     /locus_tag="JNY73_003611"
     CDS             complement(5042..6469)
                     /locus_tag="JNY73_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY8049469.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6492..7184)
                     /locus_tag="JNY73_003612"
     CDS             complement(6492..7184)
                     /locus_tag="JNY73_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY8049470.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1845 a         1764 c         1929 g         1759 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgtt gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
     1861 ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact ggctatgagc
     1921 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
     1981 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
     2041 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
     2101 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
     2161 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
     2221 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
     2281 aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc
     2341 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
     2401 ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc
     2461 aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
     2521 acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag
     2581 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg
     2641 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
     2701 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
     2761 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc
     2821 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
     2881 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
     2941 cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
     3001 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
     3061 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
     3121 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
     3181 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
     3241 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
     3301 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc
     3361 gagagcgttc gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc
     3421 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
     3481 ggtattcatg ccggcgctaa tgctgctctt tccccatcgc tggtggaagc gcaacgtgag
     3541 cgtattgcca acgcgtcagc gttattaatg cagctggaat caccactcga aagtgtgatg
     3601 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
     3661 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
     3721 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
     3781 gtactgcatg aaaagggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
     3841 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
     3901 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
     3961 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
     4021 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
     4081 cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg tttctacctc aacagtttct
     4141 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
     4201 attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca
     4261 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
     4321 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc
     4381 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggctta
     4441 ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca
     4501 gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
     4561 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
     4621 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
     4681 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
     4741 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
     4801 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
     4861 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
     4921 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
     4981 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
     5041 cttactccga ttctgatgga acgcggttcg gcatagattt acgctgtctt ttgatcaaat
     5101 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
     5161 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
     5221 aaatgcgaag gacagccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
     5281 ccgccagaac attgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
     5341 tattcatcgc ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
     5401 gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa
     5461 tcaccgtgat ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca
     5521 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
     5581 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca cagccggtcc
     5641 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
     5701 ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag aatatagaga agcagtaacc
     5761 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
     5821 gctctattcc gcttgaaaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
     5881 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
     5941 caatacctat ggggatatta attaaaaata tccagtgcca ggttgcccag gtgaccagca
     6001 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
     6061 ataccggaag aagttcatta cgaggataag cacgcagtaa ggccagccga gcaacaggca
     6121 tcatcattgc gtcgcctatc ccttgaataa cccggaagac aaccagctgt ggtagcgaat
     6181 tagaaagtgc gcaggccaga gaacccaacg tgaacagact cacggcaagg gtaaaaatgc
     6241 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
     6301 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
     6361 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg
     6421 ccgcgatcca cggcaaaccc gacatactac gcttcttttt atcgctcatt caatgtcctg
     6481 ttatcgggtt atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
     6541 tcgccatcgc tttggataat cgcatcgaca atcgcctggt gcagatccag ctttatcact
     6601 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
     6661 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc tatatcgact
     6721 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
     6781 ttgagatgcg ccttctgttc cgcggtgcca accgttgctg ccagtaggca ggcttgcggc
     6841 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
     6901 atccaccaag taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccga
     6961 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
     7021 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg
     7081 gtgccgggtt catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac
     7141 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactta tcttcctttt
     7201 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
     7261 attgattgtt tgatggcaca ttaacgggac ttttgct
//
LOCUS       DADLZM010000166         7274 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLZM010000166 DADLZM010000000
VERSION     DADLZM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.168"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            91..432
                     /gene="yneK"
                     /locus_tag="JNY73_003613"
                     /pseudo
     CDS             91..432
                     /gene="yneK"
                     /locus_tag="JNY73_003613"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            462..1211
                     /gene="yneK"
                     /locus_tag="JNY73_003614"
     CDS             462..1211
                     /gene="yneK"
                     /locus_tag="JNY73_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY8049471.1"
                     /translation="MFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMINLGAPI
                     KRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLSLLAEQESPHFQD
                     LFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKV
                     THEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            1361..2551
                     /gene="ydeA"
                     /locus_tag="JNY73_003615"
     CDS             1361..2551
                     /gene="ydeA"
                     /locus_tag="JNY73_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY8049472.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2576..3241)
                     /gene="marC"
                     /locus_tag="JNY73_003616"
     CDS             complement(2576..3241)
                     /gene="marC"
                     /locus_tag="JNY73_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY8049473.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3453..3887
                     /gene="marR"
                     /locus_tag="JNY73_003617"
     CDS             3453..3887
                     /gene="marR"
                     /locus_tag="JNY73_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY8049474.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3907..4290
                     /gene="marA"
                     /locus_tag="JNY73_003618"
     CDS             3907..4290
                     /gene="marA"
                     /locus_tag="JNY73_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY8049475.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4322..4540
                     /gene="marB"
                     /locus_tag="JNY73_003619"
     CDS             4322..4540
                     /gene="marB"
                     /locus_tag="JNY73_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY8049476.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4571..5470)
                     /gene="eamA"
                     /locus_tag="JNY73_003620"
     CDS             complement(4571..5470)
                     /gene="eamA"
                     /locus_tag="JNY73_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY8049477.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5734..6851
                     /gene="ydeE"
                     /locus_tag="JNY73_003621"
                     /pseudo
     CDS             5734..6851
                     /gene="ydeE"
                     /locus_tag="JNY73_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            6978..7073
                     /gene="mgtS"
                     /locus_tag="JNY73_003622"
     CDS             6978..7073
                     /gene="mgtS"
                     /locus_tag="JNY73_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY8049478.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1897 a         1773 c         1652 g         1952 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tatccctttt agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca
      961 atatgttatt gcactatcac caatttatga atcccaatga aagtgatctg aatgacgtgt
     1021 tgatgccagc atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aggaccctca
     1081 aactgtttgt aaaaaatgaa acgccgccaa aagttactca cgaagacctg gtgaaaaaca
     1141 ggcctcgctc ccctgtcaga ccacctatac ccgcaaccgc caaaacgcca gacctccctg
     1201 aacgtcatta aaccgtgatg ttaccgactc tctgacgcgg aacagaatca gcgtcagaga
     1261 aacggaaaac gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga
     1321 tcatttcatc actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc
     1381 ccgcaaagtg gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac
     1441 caccgaattt gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac
     1501 cgctcaggtc ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc
     1561 ttttatgtta atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt
     1621 gtttattgcc agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag
     1681 tcgcattggt gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat
     1741 ccgtatggct ccggccggga agcgggcaca ggccttgagt ttaattgcca ccggtacagc
     1801 actggcgatg gtcttaggtt taccgctcgg gcgcattgtg gggcagtatt tcggctggcg
     1861 aatgaccttc ttcgcgattg gtattggggc gcttgttacc cttttgtgcc tgattaagtt
     1921 acttccctta ctgcctagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg
     1981 ccgcccggca ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac
     2041 ggcatacagc tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc
     2101 cacggcatta ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa
     2161 actgggtaat caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg
     2221 tctggcactg ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt
     2281 ctgggggatc gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc
     2341 accagatgct accgacgtcg cgatggcgct attctccggc atatttaata ttggaatcgg
     2401 ggcgggtgcg ttggtaggta atcaggtgag tttgcactgg tcaatgtcga tgattggtta
     2461 tgtgggcgcg gtgcctgctt ttgccgcgtt aatatggtca atcattatat ttcgccgctg
     2521 gccagtgaca ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat
     2581 ggtacgtttt aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca
     2641 gcaggaaccc catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa
     2701 ttgcgccgga gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg
     2761 gcggcgcaac catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg
     2821 agctaataat catcgctatg gttcccggac cggcggtact tggcattgcc agtggtacaa
     2881 aggcaatatt ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg
     2941 aatcaatcgc tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc
     3001 cgcctgcaat tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccag
     3061 cgtaatacgc caccatcatg atggcaaata cgtacaccga ggccatcaac gactgacgat
     3121 tacgttcggc actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg
     3181 ggttagctaa cggcagcaac accaccagcc ccaggccaat tgctttaaac aaatctaaca
     3241 ttggtggttg ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg
     3301 ataaagtgtc actctttagc taaccttgca tcgcattgac caaaacttga accgatttag
     3361 caaaacgtgg catcggtcaa ttcattcatt tgacttatac ttgcctaggc aatattatcc
     3421 cctgcaacta attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg
     3481 aaattattcc attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta
     3541 acgagtatct gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc
     3601 gctgcgcggc gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag
     3661 cactgacccg tatgctggat cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc
     3721 cgaatgataa gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca atatgcgaac
     3781 aatgccatca attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg
     3841 acgaagttgc aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt
     3901 atgacgatgt ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc
     3961 gaggacaacc tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc
     4021 aaatggcacc tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc
     4081 cgcagccgta agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc
     4141 tatctggcag aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat
     4201 tactttgatg ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt
     4261 ttacatccat taaatcatta caacagctag ttgataacgt gacaacgtca ctgaggcaat
     4321 catgaaacca ctttcatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt
     4381 tgcggaacaa accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc
     4441 atcgcaggaa caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga
     4501 tgcgctcggc gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc
     4561 tcggggctta ttaacttccc acctttaccg ctttacgcca ccgcaagcca aatacattga
     4621 tatacagccc ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat
     4681 ccaacaatag tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa
     4741 cccgccaggt ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg
     4801 ccacaaacgc cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt
     4861 gaatcatgga tgcggaacca tcgagaatca gcgaggcaac aaagaaggga atgattggga
     4921 ttaaagcgct ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct
     4981 ttttattgaa gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc
     5041 cgagcatcgc cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa
     5101 tcgctaaggc gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc
     5161 caagcacgat agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca
     5221 taccaaagtt aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat
     5281 accccaagag caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag
     5341 cgaccagcat aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt
     5401 tgatgaccac aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt
     5461 ttcgcgacat tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt
     5521 tacttttctt taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata
     5581 agtcgctata cttcggattt ttgccatgcg tccgatggtt aataacaacg acgaggcaag
     5641 aagggcgctg gtagagcgtc gtagggataa gttcatttgt ctgtccggca gtgcgtttcg
     5701 ttatgtttta ttttagagat gtaaagaaat agcatggcta cgctgccatt tatgaccatt
     5761 tacttgagtc gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg
     5821 ctcactattg gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag
     5881 aaacgctata tgttactggc aattaccgcc ttcccagcgg ttttattgcc attcctttag
     5941 tgaataacgt gacgctggtt gtgctctttt ttgccctcat taactgcgcc tattctgttt
     6001 ttgctaccgt gctgaaagcc tggtttgccg acaatctttc gtccaccagc aaaacgaaaa
     6061 tcttctcaat caactacacc atgctaaaca ttggctggac catcggtccg ccgctcggca
     6121 cgctgttggt gatgcagagc atcaatctgc ccttttggct ggcagctatc tgttccgcgt
     6181 ttcccatgct tttcattcaa atatgggtaa agcgcagcga gaaaatcatc gccacggaaa
     6241 caggcagtgt ctggtcgccg aaagttttat tacaagataa agcactgttg tggtttacct
     6301 gctctggttt tctggcttct tttgtaagcg gcgcatttgc ttcatgcatt tcacaatatg
     6361 tgatggtgat tgctgatggg gattttgccg aaaaggtggt cgcggttgtt cttccggtga
     6421 atgctgccat ggtggttacg ttgcaatatt ccgtgggccg ccgacttaac ccagctaaca
     6481 tccgcgcact gatgacagca ggcaccctct gtttcgtcat cggactggtc ggttttattt
     6541 tttccggcaa caacctgcta ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa
     6601 tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca ggaatgaaag
     6661 ccagctattt ttccgcccag tctttaggct ggcttggtgc cgcgattaac ccattagtga
     6721 gtggcgtagt gctaaccagc ctgccgcctt cctcgctgtt tgtcatctta gcgttggtga
     6781 tcattgctgc gtgggtgctg atgttaaaag ggattcgtgc aagaccgtgg gggcagcccg
     6841 cgctttgttg atttaagtcg aacacaataa aaatttaatt cagccttcgt ttaggttatc
     6901 tctgctaata tctttctcat cgagatgaaa attaaggtaa gcgaggaaac acaccacacc
     6961 ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta ctgggaataa
     7021 ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac taatgaacgg
     7081 agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc ctgattttta
     7141 tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg
     7201 ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc
     7261 atcagttttc tcaa
//
LOCUS       DADLZM010000167         7186 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLZM010000167 DADLZM010000000
VERSION     DADLZM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.169"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="JNY73_003623"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="JNY73_003623"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY8049479.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /locus_tag="JNY73_003624"
     CDS             complement(986..2344)
                     /locus_tag="JNY73_003624"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY8049480.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLRELKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JNY73_003625"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JNY73_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY8049481.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="JNY73_003626"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="JNY73_003626"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY8049482.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="JNY73_003627"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="JNY73_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5309
                     /gene="msrQ"
                     /locus_tag="JNY73_003628"
                     /pseudo
     CDS             4675..5309
                     /gene="msrQ"
                     /locus_tag="JNY73_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5567..6217
                     /gene="zinT"
                     /locus_tag="JNY73_003629"
     CDS             5567..6217
                     /gene="zinT"
                     /locus_tag="JNY73_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY8049483.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2034 a         1626 c         1509 g         2017 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgacag
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga gattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgagctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctaacaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcat tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa tcaattttta aaagaatcag atgttacagc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaggtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgctgggctg
     5221 gctgtactgc tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca
     5281 agcaggccca caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc
     5341 cggccggatg cagcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc
     5401 gtttgtcatc aacctttcat caccagaata atttatttta tttcaataag atagttcaat
     5461 acacctgaga ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac
     5521 aatataacat tacacataat gtacattaac tctggaggaa attgttttgg cgattcgtct
     5581 tcataaactg gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg
     5641 tcatcattct catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt
     5701 tgatgatacc aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt
     5761 ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga
     5821 taaaaccaaa acatttgctg aaattaaaga ttattatcgc aaaggttatc cgacagatat
     5881 cgaaatgatt ggcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc
     5941 ctgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt
     6001 tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag
     6061 cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc
     6121 ccagcaatct ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag
     6181 tagcgaagaa gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta
     6241 tttaacagaa cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca
     6301 aactcagaca cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg
     6361 agaaaaaggc gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc
     6421 caggagcaag tcatctgtca tggtaaagct aaccagaaat ataggttaat tttggtgttt
     6481 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
     6541 actcttggaa gttatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     6601 ggctcaatta ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag
     6661 tttcatagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     6721 gtagattttc gctcgaaacc ttcctgtcag ctccatagcg aatcaagcgc tgaatgtcac
     6781 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     6841 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     6901 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     6961 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     7021 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     7081 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     7141 gcttattccg ttttgcctgt gtgttgaata attccactgc agttat
//
LOCUS       DADLZM010000168         7076 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLZM010000168 DADLZM010000000
VERSION     DADLZM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.170"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1168)
                     /locus_tag="JNY73_003630"
     CDS             complement(<1..1168)
                     /locus_tag="JNY73_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049484.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQELVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRL"
     gene            complement(1165..2301)
                     /locus_tag="JNY73_003631"
     CDS             complement(1165..2301)
                     /locus_tag="JNY73_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY8049485.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(2294..4574)
                     /locus_tag="JNY73_003632"
                     /pseudo
     CDS             complement(2294..4574)
                     /locus_tag="JNY73_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4585..5673)
                     /locus_tag="JNY73_003633"
                     /pseudo
     CDS             complement(4585..5673)
                     /locus_tag="JNY73_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            complement(6215..6622)
                     /locus_tag="JNY73_003634"
     CDS             complement(6215..6622)
                     /locus_tag="JNY73_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049486.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            complement(6683..>7076)
                     /locus_tag="JNY73_003635"
     CDS             complement(6683..>7076)
                     /locus_tag="JNY73_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY8049487.1"
                     /translation="RSEFAQLFADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKS
                     ATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVY
                     RDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         1671 a         1871 c         1853 g         1681 t
ORIGIN      
        1 acagccgacg cgtagctgcc ggtgagtctt cctgttcact tcgcggccaa ctgcgcgaaa
       61 ggtgcaaaat actgccggtg tcgggatcgg taaaccagat gcgtaaacca taatgctcaa
      121 tatcctgcca gcaacgcata cctaaagaca ccagccgcag atgatcaagc tttgcttctc
      181 cggcaatgcc agagccaacg accgtgcgcc acggcacagg aggaacttca ccaacactgt
      241 cgctccgggc catctcttgt gcgcaattta atcgactgtt taatgccgca agctgacgta
      301 agcattctcc ggcatgatag tggctggcgc gggcgtggaa ggcatcaacg ctggcgcgca
      361 gttgccgtag cgattcactc acccatcgcc agctgcagcg ttccgctgct tgctgcgcgc
      421 ggctgaaagc ggcctcgtag tgaataagcg gctggctgat gccgctcagc cataatgcct
      481 ggcttagttg ttgaacatat tgacgacacg ttttgccttc ttcgctggca aacggatcgt
      541 cagatgatgt gacgtgttcg ctgcgcatct gccagattaa atgagtcaat tccgcttgct
      601 gcgcttttgc ctggacaaag gcctgtactg ccagtacgac atgttcgcaa agtgtgcctt
      661 caatacaatc acaacgggcg aaacgaatac tgctgcggga ataaaaacgc acatcgctca
      721 tcggtaagcg ggcagaggga atttcacccg gcgcacagaa caactcaatg gtgatgcttt
      781 tagcgaccag ctcctgtgcg cgtttgcggg tggcatcggg aagggtagcc agttcttcca
      841 gccagattgc cggatcccat tcctcttctt ttcccgtagg ctgggcggta gcacaaagtc
      901 gttgataact taacaccagc atcacgcgat gacggcacat gccgctggcc ccacaggtgc
      961 actgagcctc tttcagtgcc tggccgttcg ccagctgggt acggacaccg tcactgaagg
     1021 tggcgattaa agcgctgttc tcatggctga tttccgggac gttgccattt tccagttcct
     1081 taaggctgcg cttaacaaaa ccggcattgc ttaacgccgt cagtgcctgc ggtgtcagtt
     1141 ctaataattc cggacgtagt gaattcatga ctgaagattc tccgcaagcc atgatgccag
     1201 ctcgcccggt gtcatggcgg ctatttgtgc gccgacatta accagcgcct gggccgtatc
     1261 gcggtcatag caaggtgttg ctgtgctatc gagcgctgcc agtcccagca ctttgatgcc
     1321 gctctggaca cactttttca cttggtgcgt cagcaatgat gatgaacccc cttcataaaa
     1381 atcgctcacg aggataatga cgctttttgc aggttgttca ataagttgcc gaccatactc
     1441 catggcactg gcgatattgg ttccgccgcc taattgcact ttcattaata actctaccgg
     1501 atcggcaacg tctgccgtga gatcaacgac gcttgtgtca aacgccacca gatgggtacg
     1561 aatgccgggt aactgccata aacaggccgc catcaccgca gagtggatca ccgaatcgac
     1621 catcgatccg ctttgatcaa ccagtaagac cagttgccat tgctcgcttt ggcgtttaat
     1681 gcggctgtta aagcggggga attcgatata caacttgccg tgttgcgggt gccagtgttg
     1741 caggttggcg cgcagagtac ttttgaaatc aaagtttcgc gccagtggaa taaatgagcg
     1801 gcgacgacga tcgcggacac cagaaaaagc ctgacgaact tcctttgcca gtcgcgccat
     1861 aatttcttcc acaacctggc gcacaatcct gcgggcggca gccagtactt cggggttcat
     1921 cagatgtttg gtgtgcaaaa cggcgcgtag caggctttcg gaaggctgca tacgttccag
     1981 cacgtcgaga tttgtcacca catcttcaat gccgtagcgc agtacggcat cgctttccag
     2041 ccgctcaatc acctgttgcg gaaacagcgt gtgaatactg ttgatccact caggagtggt
     2101 gagatttgag ccacctaatc cactagagcg ttcaccacgc tggagccgtt caggatcgcg
     2161 cccatacagc cactccagcg cgtgatctat ctgccgggcg ttgtcatcca gcccacaaag
     2221 cgtcgtttct gccgcttcgc caagaattaa tcgccagcgt tgtagctcac gggtggtcag
     2281 aagatcgttc agttcagaca tggaaaactc cccagtgttg cagcgatgcc agtgcctgct
     2341 gttcgagttg ttgatgatgt gcaatggctt gcggtggaca atgcaacggc atttgcagtg
     2401 ccgaaacggg aagttgcgcc agttgataat gctcaagcac ctgatgcgcc agcgtcccgc
     2461 gttctcgtgg cggtagccag gccattgccg cgcgtaaatc gggcagggca ttggtaaaat
     2521 cggcatcact tagttgattt aaatgactgc tgaaaccggc gataaatgcc ggctgacagg
     2581 ccagttgatg acgggctaat gccagcaaac cgtgcagcgc ctcgccggat tgtgccgggg
     2641 ataactgcgc cagcatcgtc agcgccgctt cggcactagc gttgggatgt tccagacgca
     2701 tcagcgcacc aagagtcgcg ccgcgatcca gtgccaggac atgaatcgcc tgactgtgtc
     2761 gctccagcaa ggctaccgcc gcagaaagcg aaacgccggg gagttgcaca ccgctatgta
     2821 gatcgcgcag gatatgacac aacgcttgcc agctatgcaa gtgagcgtga aactcctttt
     2881 catccggtct gccgttggat tcacacagcc acagcgtgcg atcgatagcc gcgcataacg
     2941 tcgtttgtaa tatctgcgcg ccttgcatac cgctaatttc atccagccgc cataaggcat
     3001 atagcacttc cagcgcctgg cccatttcgg caaattgatt ttcctgggcg attaataatg
     3061 tgagttgctc cagtaattgt tgactgaagg acgccagacc cgctgacgcc gcctggctaa
     3121 gacatgtggt aatgctgccg attccacccg cgtccagcat atcggcttct aatttatggc
     3181 gtgcggcttc ctggagtgtg gcaccaaagc aggcggcctc aatcaatgcc gcatgctggc
     3241 taagcgggcg ggttaatttc cagcgttctt caccgttgcc tgcaagtgtc agtgtgcttc
     3301 cctgctggcg tacaatccct gggatctcca gtattgccag ccgatgtaac acctgacttt
     3361 gcgctagtcc attggggtta aagcgattta aggtaagctc cgccggaaga gagattgcgg
     3421 tacgttccag ttcgcaagtg acatcttttg gcagaggcgg ttgtggcgta gaaggggcaa
     3481 gtttaccgaa tccgtcaccc gctaatgtgt ctatcaacgt tagcagaatc ggatcggtat
     3541 cgggatgaat aacgccgcga tagctccacg gcaacggcgc attcagggct tctttaatca
     3601 gcgatcctgc tatggcatcc agccagtcag tgcgtaacgg tagtgtatga ccgcgcaact
     3661 gtgccagtgc catcgcatgc agatgagcgg cagccatatc cgcggtcgaa gcaggcaact
     3721 tgtgctggcg caaacgggtg agaaccgttt ttagcagttg ttctcccgcc tgctgtaagc
     3781 cccactgcca gcaccagttt tgccagaccg gggcaggcat tcctgaaagg tatcctgcca
     3841 gcacatcaag gcgcttttca ctgtaggggg gtgagataac aacctgtaac ggcatctgcc
     3901 agcgacggca attctggcgt gttaattacc tgcggacatt cgcgccacat ttttgccagt
     3961 gccggagcgt gccagccacc gcagacaact aacacgtcgc cattattttg ctgcatcgcc
     4021 catccaatcc agcggaccat aaaggcttcg cgctgacgat tgagagcatc gccgggggag
     4081 tcgccccgca gttgggcaaa atagtgcgcc agtgcagagg gtaatgcagt ttgctggctt
     4141 tcatcttcga acaagtgatc ccacagggtg tcgctgttat ccatgcgggt ggcacgcagc
     4201 agcaaggtct ggctttcctc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc
     4261 cagcaaggca aatcgatgaa gtaagtttgt gcctgaatac gacgatcggc ttgtagcgcc
     4321 tgccactccg gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg
     4381 tcctgatact ggcaataact gtaaatagct accggaagct ggtgggctaa aaacagttcg
     4441 tctacccggt cattaaaatc agccgggcct tcaatcaaca cgtatcgtgg ccgctggctt
     4501 tcgattaacg atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt
     4561 aacggctcgc tcatgcatct ttcctcacgg caggcggtgg cgggcttgat aataagcctg
     4621 ccagtgagct tctttatgtg tggcgacacg ctgttcgaag taacggcgca gacgagcgcg
     4681 atcttcttcg ttatctttga cgatggttcc ggcaatgcag tccaccaggt cggctggctc
     4741 acctgcgcga ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc
     4801 ttcagcggtg gacataatcg ctgttagtgt atccattgac gttttctttt cgccgttggc
     4861 acgcagatcg cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg
     4921 aataccgcta tgggccagca aacgcgccga agcactggcg acaagctcca gctcctgggc
     4981 gaaatccata atcggaaaga cagtttcaaa atcgaagcga cgctttagcg cagcactcat
     5041 ttcattgacg ccgcgatcgc gggtattggc agtggcaata atattaaagc cttctcgcgc
     5101 atagagctga ctggcttcac cagtgagttc cggcaccgtc atcaccctgt cagaaagcat
     5161 acctaacaga cagtcttgca cttccagcgg tgtacgcgtt atctcttcaa aacggacgat
     5221 tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc agcgcttccg ttgatggacc
     5281 atgattgata agcagggcgt agttccagcc gtacttgatt tgatcttcgg tagtagatgc
     5341 gccgccctgg atggttaacc cggcatcgcc gctaatagag gttgccagta attcagacag
     5401 gagagatttt gcggtgctag gttcccccac caacatcaaa ccgcgtccgg tggcaagagt
     5461 gactaacata cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc
     5521 gctatcgcca aggataaaag cacgggccgc aggcaaactt aactaccaac cgggcgggca
     5581 aggtgcgtta tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg
     5641 cggacgctgc agatgattat tctgcggtga catatattca tccctgaaat gtattaatga
     5701 aataaacgat attttacgcg caataaatgt ttggcgcatt agttgtttga gcgaaaaata
     5761 tctggaaagc agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta
     5821 ctattttcag gcctaacacc actgcctgtg caacatcgtg ttccaacgaa cgaattataa
     5881 ccacctctgg acagtgttgc aattaagata atgagattta taatcctgac tgttgggaag
     5941 ttgcgccgga tcgaacccat ttcaagtcct ggctaccgga cgggtggtgc cgcaggtagt
     6001 gctctgatcc ggagtgcaac atggcgactc aacaaacgta aaccagtatc gacaggcagt
     6061 gtaagggtaa ggtgctggtg ttgtgctcct taataaggag agcggatgga cacgctgaca
     6121 caaaagttaa ccgtgctcat tgctgtactg gagctattag tggctctgtt acggttgatt
     6181 gatttgttga agtaacgggc aaattgaaac acatctaagg gggaataccg ttcccccctt
     6241 taataaagct atactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttatctagag
     6301 tatataattc tatttgttgg ttgaatatat tctgtagttt acaattaaaa attactatta
     6361 caggttcagg tgattcgcct cgtaattttt gctcatcaat aaactcatct cttatataaa
     6421 tcaacgcatc atttattagc gggtcaacat cctctcgatc acgttgatga aatgttggac
     6481 gtagccggaa ccgttccagt cggtagacgg aacctgtaaa taattcagag catttatgga
     6541 tctcaagtct gaaggaaaac ggatcttcag gtacagggac ctcataatcg taaatagcaa
     6601 ccagctcttt ttgcacgatc atcgctttcc ccgctggtaa gtcgtgtaga taacgggcag
     6661 tcgcatcact gcccgctttg ttttaatgga aaatgacgtc aggtgcgctt aacgcctcac
     6721 tcaaatcaaa cacatcaagt tcggcaaaag tgacggattt attgtcacgg tacactcgaa
     6781 gagaacggaa gctcattagc gctttgctat caacattgta gtggttaaaa ccgggtgaaa
     6841 acttaaggac cagccggtac tcacccaggt catagacaaa tgcgtcctca taacctgact
     6901 cccagccttt gtatcgcatt cccataagtt gcccaacggt agcggattta ccttcccagc
     6961 gagtcaggct gttactggtt gactcgtcag gtgtgagcag caccgtgcgg cgcgacaact
     7021 ggcgaaaagg cggcataatt tcgtaatcgg caaaaagctg tgcaaattca ctgcgc
//
LOCUS       DADLZM010000169         6812 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLZM010000169 DADLZM010000000
VERSION     DADLZM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.171"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..730
                     /locus_tag="JNY73_003636"
     CDS             <1..730
                     /locus_tag="JNY73_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY8049488.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
     gene            complement(1033..1803)
                     /locus_tag="JNY73_003637"
     CDS             complement(1033..1803)
                     /locus_tag="JNY73_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049489.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="JNY73_003638"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="JNY73_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="JNY73_003639"
     CDS             complement(2294..2515)
                     /locus_tag="JNY73_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049490.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="JNY73_003640"
     CDS             complement(2516..3199)
                     /locus_tag="JNY73_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY8049491.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="JNY73_003641"
     CDS             complement(3263..3574)
                     /locus_tag="JNY73_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049492.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3571..4542)
                     /locus_tag="JNY73_003642"
     CDS             complement(3571..4542)
                     /locus_tag="JNY73_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY8049493.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="JNY73_003643"
     CDS             4913..5872
                     /locus_tag="JNY73_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY8049494.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="JNY73_003644"
     CDS             5872..6267
                     /locus_tag="JNY73_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY8049495.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..>6812
                     /locus_tag="JNY73_003645"
     CDS             6450..>6812
                     /locus_tag="JNY73_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY8049496.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAE"
BASE COUNT         1861 a         1758 c         1662 g         1531 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctgggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgctcaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcacagg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtag cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cgcaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgcg tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga cgccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat cgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggcgtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca cacagataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc cagcgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggttcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaagta gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgcttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgat
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg acccccgact gttaacgcta
     6061 atcagcggtt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaataaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aa
//
LOCUS       DADLZM010000170         6796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLZM010000170 DADLZM010000000
VERSION     DADLZM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.172"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            239..2671
                     /locus_tag="JNY73_003646"
     CDS             239..2671
                     /locus_tag="JNY73_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY8049497.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGLFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2702..4207
                     /locus_tag="JNY73_003647"
     CDS             2702..4207
                     /locus_tag="JNY73_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY8049498.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGNAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4207..5868
                     /locus_tag="JNY73_003648"
     CDS             4207..5868
                     /locus_tag="JNY73_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY8049499.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="JNY73_003649"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="JNY73_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY8049500.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..>6796)
                     /locus_tag="JNY73_003650"
     CDS             complement(6477..>6796)
                     /locus_tag="JNY73_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049501.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2007 a         1613 c         1551 g         1625 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgg
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcctatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca tgctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcaatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaaggaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga ataaca
//
LOCUS       DADLZM010000171         6781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.173, whole genome shotgun sequence.
ACCESSION   DADLZM010000171 DADLZM010000000
VERSION     DADLZM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.173"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..3524
                     /locus_tag="JNY73_003651"
                     /pseudo
     CDS             1..3524
                     /locus_tag="JNY73_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6749
                     /locus_tag="JNY73_003652"
     CDS             3534..6749
                     /locus_tag="JNY73_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY8049502.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSPEWQSAITQAINLIGEHKPSVPARTMAALAFIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAVVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPSYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSQMPEILVSPPWRGRKKKTSIRHFSLPD
                     LTLAPQTYRSPNKHWTPEQQTAIDHFSAMSFDERLATRGADIFLRELGFDVHSLEFNK
                     RLLNGQLDAFDAQQLQEYTSIGLLRTYHAHQAKSKQYREDATNALLAQDSIALMGGWR
                     TFQHGFYYGNEQRWGIWNLYLIAQMPREMAVSCWQQIVEADFHYTGVEYLLSVLGTDA
                     LPGLNTAFARHPKEIFPLLIHFGATELALPIARVWHLFAGQRNLARQWILQWPEHTAT
                     ALIPLVFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPI
                     EIYPARIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQL
                     KTFCQPQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTHILAWPQE
                     GKSARAVSGLNILTLMNNDMALMQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEE
                     LADRLVPTLGLNDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQ
                     LKAPEALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQ
                     RLIWGVIRQMNRVVYSTPFVWPQRGSSAMRKMSQLTCLRTL"
BASE COUNT         1797 a         1781 c         1634 g         1569 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 a
//
LOCUS       DADLZM010000172         6780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLZM010000172 DADLZM010000000
VERSION     DADLZM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.174"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            337..579
                     /locus_tag="JNY73_003653"
     CDS             337..579
                     /locus_tag="JNY73_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049503.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            693..1331
                     /locus_tag="JNY73_003654"
     CDS             693..1331
                     /locus_tag="JNY73_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY8049504.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQSTLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1328..2065
                     /locus_tag="JNY73_003655"
     CDS             1328..2065
                     /locus_tag="JNY73_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY8049505.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPFTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            2065..4161
                     /locus_tag="JNY73_003656"
     CDS             2065..4161
                     /locus_tag="JNY73_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY8049506.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ERMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4208..4486)
                     /locus_tag="JNY73_003657"
     CDS             complement(4208..4486)
                     /locus_tag="JNY73_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049507.1"
                     /translation="MKRNLIKVVKMKPYFATLMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4487..4630
                     /locus_tag="JNY73_003658"
     CDS             4487..4630
                     /locus_tag="JNY73_003658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049508.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4756..5166
                     /gene="psiE"
                     /locus_tag="JNY73_003659"
     CDS             4756..5166
                     /gene="psiE"
                     /locus_tag="JNY73_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY8049509.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="JNY73_003660"
                     /pseudo
     CDS             complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="JNY73_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
BASE COUNT         1698 a         1696 c         1785 g         1601 t
ORIGIN      
        1 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
       61 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
      121 tacgcagatt aattactttt gtttttattt taagtttatg atttttattg ttatttaaat
      181 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
      241 ctcgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
      301 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
      361 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
      421 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
      481 accgtaagct cggcggtggg tgtcgcgctc gcggtaaccg gtggcggtga tggttctaat
      541 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
      601 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
      661 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
      721 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
      781 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
      841 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
      901 aagacggaca acaaaaatgg gtcactcagg atcaatccac tcttgtcaca caacatggtc
      961 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     1021 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     1081 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     1141 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     1201 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     1261 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     1321 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     1381 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     1441 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     1501 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     1561 gctgacacgg ctggcagagc agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     1621 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     1681 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     1741 gtgggtcgga ccaccgccgt tcacggtcac gttgttcggg cttatcagcc atcctggcaa
     1801 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     1861 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     1921 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     1981 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     2041 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     2101 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     2161 gatttcggtg gcgtaggatt attacaaact cccaccgagc gtatggcacg ggaaggggag
     2221 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     2281 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     2341 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     2401 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     2461 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     2521 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     2581 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     2641 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     2701 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     2761 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     2821 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     2881 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     2941 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     3001 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     3061 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     3121 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     3181 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     3241 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     3301 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     3361 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     3421 gatttgtggc tgacggacca tctactgacc actggcagcc tgtttgcaaa tcttgccaac
     3481 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     3541 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     3601 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     3661 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     3721 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     3781 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     3841 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     3901 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     3961 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     4021 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     4081 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     4141 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     4201 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     4261 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     4321 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     4381 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagtag
     4441 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     4501 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     4561 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     4621 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     4681 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     4741 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     4801 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     4861 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     4921 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     4981 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     5041 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     5101 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     5161 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     5221 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     5281 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     5341 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     5401 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     5461 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     5521 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     5581 ccgagcctga gtgtaaaacg cggtactgag gctaaacata ccgattgcca ttccgagtgc
     5641 gccgataatt tgcagtggct tacgaccaaa tttatccaca gtcataattg ccagtacggt
     5701 aaaggtgagg ttgataactc cgacaataat ggtctgcaac agcgcgatat ccgtgctggc
     5761 ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc acattgatgc cgacaaattg
     5821 ctggaagatg gagagcataa cgccgattac aatcacgccc acgccaaaca tcagcagacg
     5881 accaccggtt ttgcggccat gatccaggga gtgtttaatt tcctgtactg cctgagttgc
     5941 aagcgtgttg cccataattt tgcgcaggat actttccgtc tgttcttgct tgccgcgtga
     6001 catcagccag cgaggacttt ctggcacggt atacagcagc attaagaaca gcagtgcagg
     6061 gatacattcc gaggcaaaca tataacgcct gccgtcagta ttcagccagc tggcatcacc
     6121 ggaacgggca ataaaatagt ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa
     6181 ctggttaaat gaaaccagtt ttccgcgaat atgagctgga gccagttccg caatatacat
     6241 tggtgaaagc attgaggcta aaccaacgcc aataccgcca ataatacgat aaataacaaa
     6301 ttccgggaca taacctgcca gataaatagg tactgtgttg tccgggttta tagaggtaaa
     6361 accaagttct ggccaggcag aacctacacc agaaataaaa aacaggacag cagaaatctt
     6421 aagtgaatca cgacggccga agcggttact gcaataacca ccgagggcac cgccgatgat
     6481 gcaaccaatc agagcgctgg ctacgcaaaa tcctaacagg gagttggcag cggattcact
     6541 taagttttgt ggagcaacaa agacggtatt gagtgactca acagtaccgg aaataacggc
     6601 ggtgtcgtag ccaaataata aaccacctaa tgtagcgact aaggtaatcg aaaatatata
     6661 actggaacta tactgggtat tcattcagac ctgccttaga ccattctgat cttttatcag
     6721 tgcattgatg ttggaattgt caggcagtta aggcaattat cattttttac aatgcgttta
//
LOCUS       DADLZM010000173         6585 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLZM010000173 DADLZM010000000
VERSION     DADLZM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.175"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1282)
                     /locus_tag="JNY73_003661"
     CDS             complement(<1..1282)
                     /locus_tag="JNY73_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY8049510.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQ"
     gene            complement(1567..1968)
                     /gene="pemK"
                     /locus_tag="JNY73_003662"
     CDS             complement(1567..1968)
                     /gene="pemK"
                     /locus_tag="JNY73_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HAY8049511.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(1901..2158)
                     /gene="pemI"
                     /locus_tag="JNY73_003663"
     CDS             complement(1901..2158)
                     /gene="pemI"
                     /locus_tag="JNY73_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HAY8049512.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2251..2904)
                     /locus_tag="JNY73_003664"
     CDS             complement(2251..2904)
                     /locus_tag="JNY73_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAY8049513.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(3002..3142)
                     /locus_tag="JNY73_003665"
     CDS             complement(3002..3142)
                     /locus_tag="JNY73_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049514.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            complement(3842..4699)
                     /gene="repA"
                     /locus_tag="JNY73_003666"
     CDS             complement(3842..4699)
                     /gene="repA"
                     /locus_tag="JNY73_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY8049515.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(4692..4766)
                     /locus_tag="JNY73_003667"
     CDS             complement(4692..4766)
                     /locus_tag="JNY73_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY8049516.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(5011..5259)
                     /locus_tag="JNY73_003668"
     CDS             complement(5011..5259)
                     /locus_tag="JNY73_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY8049517.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(5544..5693)
                     /locus_tag="JNY73_003669"
     CDS             complement(5544..5693)
                     /locus_tag="JNY73_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8049518.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(5940..6413)
                     /locus_tag="JNY73_003670"
     CDS             complement(5940..6413)
                     /locus_tag="JNY73_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049519.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
BASE COUNT         1717 a         1605 c         1453 g         1810 t
ORIGIN      
        1 cctgtttaaa atcataattt ggttgttctg ttcttgagtt attaagtaga gttcttatca
       61 gtgtttgcca agcatgattg ataaatttat tttctgattc tttaaaatat cgtctgatta
      121 aagcacataa atcttcgaca cttttctgac gagcgctagc tgcccaacgg cccccttcgg
      181 tttgcaccat tgaacgttgt acactgtctc ctaactgagc gataaatttc tgttcatcgt
      241 caagttgcat gttttcattt atgattaatt catacaaagc aagaaaaaca gcttgatagt
      301 aacgaggaca ttcttgagat gaagatgcat tgcccagaat gtgtgatcta aagttcaaat
      361 tatttgcatc aaataatttt tgaatcatct cataaacgaa gaggtaatta ttgacaattt
      421 tatctgcacc atttgtctga atgtaattct ctaattgagt tcgtgtatgg attgcatgac
      481 tcgtcgaacc ttcaccgtaa aatgtatcga gcgatgttga tcctgatgtt ggcttatcat
      541 ctaaaaatat atagcccaag atatttgcaa ctaactcttc atcgtcagag tctctaatgc
      601 taggcctact aatgatatga tttctaatat agaaatgatt gtctatattt attccataat
      661 cgagtccatc attgcaaatt gatatgttat gtattttatt cagtcccatt atatcgctat
      721 gtgtagtatc tcctcttata attgtcgata ttttccttac taactctgaa aaattactaa
      781 cgcaccccgc acttcgtagt tcttgttttg ataaaatttt accattggaa tttatgcgtc
      841 gaaacacttc gtcaatgtgg tcctctgttg aagagtattc agatatagca aatctatagc
      901 gagcaattaa tgtagaactt tcgcgggata gcactggctc tttttgttga agtttaccac
      961 tgtcttttag ttgttttgtt aatgcaatgg catctaaatt aaagtattta ccatttaatg
     1021 aaaatcgctg ctcaataaaa ctcattaaag cttctagtct ttgcattcca tcaaggattt
     1081 ctcttttgtt gtcaatggaa acaaacaata gcaaagggat agggtattgc tgagtcattg
     1141 atgaaatcag tgctgttttc tcttcgaggg accatactaa ttttctttgg tatcttcgat
     1201 ttataattaa tttatcatcc agataccact cgtataattc ttctgctgtc ttggagtcaa
     1261 tatctaagtt ttcattagcc attagatgta tctcgagtat ttagttaaga aactgatagg
     1321 tcagtatttg catcaatgtt cattgaattt gtatccgaag tcaatctgat gtaggaactt
     1381 caaaactgcg caaaacacgc aaatacgcac aagaaagtag ggctgcgctt ggaattcgga
     1441 aaataaacca catcaaaact actttttagc caaataacgt ttgggaatca ccagaatggt
     1501 gggacaacag cggttttagt gccctaaatc gtacgttttc atccagttgc ccctcaaacc
     1561 ccatgttcaa gtcagaatag tggacaggcg gccaagaact tcgttcatga tagtctccgg
     1621 aacccgttcg agtcgttttc cgccccgtgc tttcatatca attgtccggg gttgatcgca
     1681 acgtacaaca cctgtggtac gtatgccaac accatccaac gacaccgcaa agccggcagt
     1741 gcgggcaaaa ttgcctccgc tggttacggg cacaacaaca ggcaggcggg tcacgcgatt
     1801 aaaggccgcc ggtgtgacaa tcagcaccgg ccgcgttccc tgctgctcat gacctgcggt
     1861 aggatcaagc gagacaagcc agatttcccc tctttccatg tcagatttcc tcctgaccag
     1921 tcgccggtgc atccagccat tctcgttctt cagctgatat ttcagcattc ggatcacact
     1981 gtgccagtag ctcagccagt gaatattgcg ggcgtctgta cggctcaaca atcagccggc
     2041 cattatcaat gaccatgcca acttcattat ctgtgcccag agacagcgca ttcagcagtg
     2101 ccggtgggac ggtcagcata actgagccgc caaccctctt cagtcgggtg gtatgcattc
     2161 ttcacctcca taaaagttat atttaaatat aacatccact aaaaaaacac accaggcttt
     2221 aacgcacaat gtttaataaa aatataactt tcaaccaaac agtaaaccca gcgtggttgc
     2281 ttccatatgc agcaataccg gcagcagcag gccaccactt ctgatcctgg ccactgatgt
     2341 aatcaacccc accaggaaca gttctgccag tgtcagcagg ttctgatact ggctgtgcgc
     2401 ggcgacgaac aacaacgacg ttatcagcgc ccccagccac atcgtccagc agtaccgtga
     2461 acggaagacg ttcagcataa tcccccggaa cagcgtttcc tcattcaacg gggcaaggat
     2521 aaagatggtc agcaacgtca ggatcacgtc aggtatggac ttatcggcaa aaagtttcgt
     2581 cataaatggc tcagcaggca gagccagcgc cttaccgagc agaaatacac cgacatacac
     2641 cacggccatc gcaccgacca gccacggtac gccaacgttg cgcagctgac caacgaccgg
     2701 tagcggagct atccaacggc ggtataccag gaaaacacac agcaggtaca tcagaacagt
     2761 accatgactg aagaacaaat agttttttcc tgatccataa agcagaacgg cctgctccat
     2821 gacaaatctg gctccccaac taatgcccca ggcagccagc ataaccagca taaactgcag
     2881 atattgatta cgtgtttgaa tcattgcatc gcctgtaaat ttttaacttg tcctattttt
     2941 gtcattacca cgtatataca catgtataac aattcagata tcgttaccag gatatgccgc
     3001 atcagcggca tggaaggcgg cactctgttg tttcatatga tacaggagta aaaccgccga
     3061 agcccggcgt aagccggtac tgattgatag atttcacctt acccatcccc agccctgcca
     3121 gaccataccc gctttcagcc atgagagagc ttctgtgcgc ggtcggagtg gtcccgacga
     3181 gggtttaccc gaagtcgggg cgtatctccg cgttagcggg ccgtgagggc cgcttacgag
     3241 cgtgtactga gaacttccag cgagaagact gacagcgatg aagatgtagt tacaacattc
     3301 ataattaaaa gcgactctgt tccggccctt cgggccgggg cggggccgct tttcagttat
     3361 gaggaggggc tttgtggttt cagttctgcg ctggactggg gttttcccgg tgcttgtatt
     3421 tgagtgttgt aactaaaatg gctccggtcg gggcccgccg cttgcggtgg gaggtgcata
     3481 tctgtctgtc cacaggacag gcagtgaata ggttttcttt ttaaatgaat gtaattaagt
     3541 agtttaaagg agatataaac aggtgtttaa aagatacatt gcaccctgta agactggcgg
     3601 ctggcgcttt atgacatgaa cggttgtaac cttatgggga agtcccttgc agtttaatgt
     3661 ggataagcaa aattccccgt ctgtgaggcg tgttttgtat cgaaaacagg gggggccgga
     3721 tgcaccagaa gctggatgat gaggttgttt tttgtatgtg gtgctgtttt tttgtgcatc
     3781 gacgggcttc aggcgtgcga atgcctccgg cgcgggccgg attattcaga ggaggtcact
     3841 ttcaggggga agctgtggcc agccggctgt aattacggtt acgtgacaga atcatgcgct
     3901 ccttcacacg acgctcaact tcgcgtttta ccgcctcacg attggcagtg aagcgccctt
     3961 ccgcgatttc gcgcgtcagc tgccgtttca ccagggtgac aatatcctga cgttccctgt
     4021 ccgcatcacg acgcgcacgg gcacgcttta ttccccggga cttaagctct gtctgataac
     4081 tgcgaaaacg ctcacgaaca aaacgccagg ctttcgctat cagttcatcc atgcccaggg
     4141 tatccagccc ctgctttttg cgttgtttgt tttcccatac cacacggctg cggcgcgcgg
     4201 cggccgctgc ctcctctgat acatcgaggg cagcaaacag tgcagatgtg aacgtgatat
     4261 cggtcggaat gtagcacccg ataagcgggt catattccgt ctggtaggta atcagtccca
     4321 gctctgacag gaacgtcagg gcccgggtgg cacgggtgat ggagagtttt ccggcagcag
     4381 actccgtcgc cagtccgcac tcaatggcca gcgtggtgat ggagcactgg acgcggttgg
     4441 ccagcgggtc atagtggaaa cacagcccct gcaggagcgc atcaatagcc cgtcgacgca
     4501 gcaccggtgg catgcgtcga cgcagaccac gcgaacgggc atgcgccaca tgaatggcga
     4561 aatcaaaacg ggaagtgaag cccaccgcct tttccatcag tttttcgcag aacttcagcg
     4621 ttccggcacc tttacggggt gtaaacaccg gattcgggtt ctttacctgg cggtaatacg
     4681 tttgttgaag atcagtcaca ccatcctgca cttacaatgc gcagaaggag tgagcacaga
     4741 aagaagtctt gaacttttcc gggcatataa ctatactccc cgcatagctg aattgttggc
     4801 tatacggttt ttagatggcc ccggtaatct tctcagtcgc caaacttgtc gaagattatc
     4861 ggggttttca cttttctggc tcctgtaaat ccacatcaga accagttccc tgccacctta
     4921 cggcgtggcc agccacaaaa ttccttaaac gatcagcaat ctatcacgca caccagaaac
     4981 aggcaagaat gtgatgtcgc tacattttag ttagctaaaa gcactcttct gctttatcag
     5041 ttcggcaatc atctcagcct gggtaacgcc ctcagcttca cacatcacct gcaattgcgc
     5101 ttttaacgca gcaggaatga acactctgag ttctttatgt gtagctcgtt ttcttgccag
     5161 ggatgcctgc tgacgttctg caagcgtcag aggattacct tttctgtatg ctcgctttgt
     5221 acctgatgag gaagtcactg cattttctgt ctgcgacatc tcgcctcctc aatactcaaa
     5281 cagggatcgt ttcgcagagg atactacagt tttttgaaat cagcgacttg agaattgtga
     5341 cgaagatctg tgttggcgca caaatcaacg gggattactg ttgtttaatg tgatttttgt
     5401 tgcgaaatgg ctgaaaaaac aggaaatgcc ttaacccggg agccggcccc gggcgggacc
     5461 ggctgtttct ggcatgtcgg gtgtctgccc ctgaaatacc tcacacttcc ggaaagcggg
     5521 gacttccgtc cccgcccggc agtttacttc cgtgcttcgt aggccagagt tacctgcacc
     5581 actgtatttc ccctgtgaat attcagctca cataaccgtt ccctgaatat cagtgaaaaa
     5641 cacaacaccg tggcgcacac ggcgagcaac ccgataaggg catacttcgt cataaacttt
     5701 gacctctctg caaggaagag gctacaatca gtagtgcaat gtacttcatg cagacctcac
     5761 gagttaatgg attaacaagt ggggtctttg catttctggt acctgcgtct tgcctgaacc
     5821 agaaaggctc agacacccgc cgcgataata ccacattccc tgttgctgac tattacctgc
     5881 attgcccgct ttttactaca gaagcacgaa ggcgtcactc cagccaataa gtatgctttt
     5941 tatttacatc catttttaat ccggtaccga cgttttcctc cagttaactc gtttttcgta
     6001 tgactcatgg caataaaatt ttctttacca tatgacaaca tcccaataat atcaaagttg
     6061 tcttttaatt tgatggcatc cttttcactt acagcgacaa taagttgttt accaaatttt
     6121 ctgaccatca ttgcaaactg tgatactgct gatccaccac ctaaatcagg ccacccagca
     6181 caattaaaaa tgattgtctg ctcttcacta ttcattgcat ccctgataaa ggagacagca
     6241 tcctcatttc tctctacatc tgaatcactg ttaatctgag tgaaatcata atatttagaa
     6301 cctggtatag aatacccctt aaaaaactta tcaatgtggg aggcagagtt cccaacaata
     6361 agaatattgt gttcacgctt aatcatcaat tctgatacat catcatattt catatattca
     6421 ccattattat ataaagtttg ggtacaggtg gttccggaat ttactctgtg accaccattt
     6481 cccgcttttt attacagaaa cgccgggagc ggggaaaact tgctttttcc cgtttccggg
     6541 gttggacaac tgagcaacgc gaaggcgtca gctacgatgt tccgg
//
LOCUS       DADLZM010000174         6524 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLZM010000174 DADLZM010000000
VERSION     DADLZM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.176"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1968
                     /gene="acnA"
                     /locus_tag="JNY73_003671"
     CDS             <1..1968
                     /gene="acnA"
                     /locus_tag="JNY73_003671"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY8049520.1"
                     /translation="MLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGV
                     VGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVE
                     LVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAAS
                     NELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKA
                     VTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTQYLDELGFNLVGYGCTTCIGNSGPLP
                     DPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAS
                     EPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRS
                     DTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPD
                     SPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPD
                     SDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIH
                     RSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV
                     VPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2032..2622)
                     /gene="ribA"
                     /locus_tag="JNY73_003672"
     CDS             complement(2032..2622)
                     /gene="ribA"
                     /locus_tag="JNY73_003672"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY8049521.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2792..3556
                     /gene="pgpB"
                     /locus_tag="JNY73_003673"
     CDS             2792..3556
                     /gene="pgpB"
                     /locus_tag="JNY73_003673"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY8049522.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3705..4013
                     /gene="lapA"
                     /locus_tag="JNY73_003674"
     CDS             3705..4013
                     /gene="lapA"
                     /locus_tag="JNY73_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY8049523.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4020..5189
                     /gene="lapB"
                     /locus_tag="JNY73_003675"
     CDS             4020..5189
                     /gene="lapB"
                     /locus_tag="JNY73_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY8049524.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAVADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5382..6119
                     /gene="pyrF"
                     /locus_tag="JNY73_003676"
     CDS             5382..6119
                     /gene="pyrF"
                     /locus_tag="JNY73_003676"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY8049525.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6119..6445
                     /gene="yciH"
                     /locus_tag="JNY73_003677"
     CDS             6119..6445
                     /gene="yciH"
                     /locus_tag="JNY73_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY8049526.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         1595 a         1522 c         1771 g         1636 t
ORIGIN      
        1 atgttaggcc agccggtttc catgcttatc ccggacgtag tgggctttaa acttaccgga
       61 aaattacgtg aaggtattac cgccacagac ctggttctca ctgttaccca aatgctgcgc
      121 aaacatggcg tggtggggaa attcgtcgaa ttttatggtg atggtctgga ttcactaccg
      181 ttggcggatc gcgccaccat tgccaatatg tcgccagaat atggtgccac ctgtggcttc
      241 ttcccaatcg atgcagtaac cctcgattac atgcgtttaa gcgggcgcag cgaagatcag
      301 gtcgagttgg tcgaaaaata tgccaaagcg cagggcatgt ggcgtaaccc gggcgatgaa
      361 ccgattttta ccagtatgtt agaactggat atgaatgacg ttgaagcgag cctggcaggg
      421 ccaaaacgcc cacaggatcg cgttgcactg cccgatgtac caaaagcatt tgccgccagt
      481 aacgaactgg aagtgaatgc cacgcataaa gatcgcctgc cagtcgatta tgttatgaac
      541 ggacatcagt atcagttacc tgatggcgct gtggtcattg ctgcgataac ctcgtgcacc
      601 aacacctcta acccaagtgt gctgatggcc gcaggcttgc tggcgaaaaa agccgtaact
      661 cttggcctca agcggcaacc gtgggtcaaa gcgtcgctgg caccgggttc gaaagtcgtt
      721 tctgattatc tggcaaaagc gaaactgaca cagtatctcg acgaactggg gtttaacctt
      781 gtgggatacg gttgtaccac ctgtattggt aactctgggc cgctgcccga tcctatcgaa
      841 acggcaatca aaaaaggcga tttaaccgtc ggtgcggtgc tgtccggcaa ccgtaacttt
      901 gaaggccgta tccatccgct ggttaaaact aactggctgg cctcgccgcc gctggtggtt
      961 gcctatgcgc tggcgggaaa tatgaatatc aacctggctt ctgagcctat cggccatgat
     1021 cgcaaaggcg atccggttta tctgaaagat atctggccat cggcacaaga aattgcccgt
     1081 gcggtagaac aagtctccac agaaatgttc cgcaaagagt acgcagaagt ttttgaaggc
     1141 acagcagagt ggaaggaaat taacgtcaca cgatccgata cctacggttg gcaggaggac
     1201 tcaacctata ttcgcttatc gcctttcttt gatgaaatgc aggcaacacc agcaccagtg
     1261 gaagatattc acggtgcgcg gatcctcgca atgctggggg attcagtcac cactgaccat
     1321 atctctccgg cgggcagtat taagcccgac agcccagcgg gtcgatatct acaaggtcgg
     1381 ggtgttgagc gaaaagactt taactcctac ggttcgcggc gtggtaacca tgaagtgatg
     1441 atgcgcggca ccttcgccaa tattcgcatc cgtaatgaaa tggtgcctgg cgttgaaggg
     1501 gggatgacgc ggcatttacc tgacagcgac gtagtctcta tttatgatgc tgcgatgcgc
     1561 tataagcagg agcaaacgcc gctggcggtg attgccggga aagagtatgg atcaggctca
     1621 agccgtgact gggcggcaaa aggacctcgt ctgcttggaa ttcgtgtggt gattgccgaa
     1681 tcgtttgaac gaattcaccg ttcgaattta attggcatgg gcatcctgcc gctggaattt
     1741 ccgcaaggcg tgacgcgtaa aacgttaggg ctaaccgggg aagagaagat tgatattggc
     1801 gatctgcaaa atctacaacc tggcgcgacg gttccggtga cgcttacgcg cgcggatggt
     1861 agccaggaag tcgtaccctg ccgttgtcgt atcgacaccg cgacggagtt gacctactac
     1921 cagaacgacg gcattttgca ttatgtcatt cgtaatatgt tgaagtaaca agtatttgct
     1981 tgccggttat tttgcttccg gcaagcaaat gaattacaca atgcaagagg gttatttgtt
     2041 cagcaaatgg cccattttct cggctttggt atcgagataa tgttcgttat tggggttacg
     2101 acctacaatc aatggtacgc gttcaacaat attaatccct gcttcggtca gaatttcgac
     2161 ttttttcggg ttattggtta acaagcggac ttcattgacg ccaaggagtt tgaacatatc
     2221 agcgcaaaga gtgaagtcgc gctcatcagc ggcgaagcct aactggtggt tagcctctac
     2281 ggtatcgtaa ccttgatcct gcagtgcgta agcgcggatt ttattcagca gaccaatgtt
     2341 acgaccttcc tgacggtgat acagcaaaat accacggcct tcctcggcaa tttgcgtcaa
     2401 tgccgcttcg agctggaagc cacaatcgca gcgcaagctg aacagggcgt caccggtcag
     2461 acattcggaa tggacgcgcg caagtaccgg ggtatgcccg gaaatatcgc catagactag
     2521 cgcgacatga tcgtgtccgg ttgccagttc ttcaaatccc accatcagga aatcgcccca
     2581 tggggttggc agtttggctt ctgccacacg tttaagctgc atgaaattct ccagataatg
     2641 ctggttctgt attggcttat tttgccataa cgagaagggg ttcacctaat cacgacgcgt
     2701 cgatcgttca cggaatggca caaatctgtc aatttttctt ggaactggca ttttcagtta
     2761 tgattgtggg acttatcaaa aaggagaggc catgcgttcg attgcccgac gtaccgcagt
     2821 gggagctgca ctattgtttg tcatgccagt agccgtatgg atttctggct ggcgttggca
     2881 acctggagaa caaagttggc gactaaaagc ggctttttgg gttactgaaa ctgtcaccca
     2941 gccctggggc gtcattacac atttgatttt gttcggctgg tttctctggt gtctgcgttt
     3001 tcgcattaag gctgcctttg tattattttc cattctggcg gcggcaatcc ttgttggaca
     3061 aggcgttaaa tcctggatca aagacaaagt acaggaacca cgaccttttg ttatctggtt
     3121 ggaaaaaaca catcatattc cggttgatga gttctacact ttaaagcgag cagaacgcgg
     3181 aaatctagta aaagaacagt tggctgaaga gaaaaatatt ccacaatatt tgcgttcaca
     3241 ctggcagaaa gagacggggt ttgcctttcc ttccggtcac acgatgtttg ctgccagttg
     3301 ggcactgctg gccgttggtt tgctgtggcc gcgtcggcga acgttaacca ttgctatctt
     3361 gttggtctgg gcaacgggag tcatgggaag ccgcctgcta ctcgggatgc attggccacg
     3421 cgatctggtg gtagctacgt tgatttcgtg ggcgctggtg gcggtggcaa cgtggcttgc
     3481 gcaacgaatt tgtgggccat taacaccacc tgcggaagaa aatcgcgaaa ttgcacaacg
     3541 agaacaagaa agttaacgct ggttgatttt ccgaatttag gccttaaatc atcaacaatg
     3601 cgcgtggatg ccatttcgca gacggcgcga aaatggtact ttaaagggct attgcggtaa
     3661 gttgaccata atttattcgc tctaaccaca taacgggaag taatgtgaaa tatttactca
     3721 ttttcttact ggtgttagcg atcttcgtga tttcggtcac gttgggtgcg cagaacgatc
     3781 aacaggtgac gtttaattat ctgttagcgc aaggggagta ccgtatttcc acattgctgg
     3841 cggtattgtt tgctgcgggg tttgctatcg gttggttgat ttgtggcctg ttctggctgc
     3901 gagttcgtgt ttccctggcg cgcgctgaac gtaaaataaa gcgactggaa aaccagcttt
     3961 cacccgcgac tgacgtggct gtagtgccgc actcgtcagc ggcgaaggaa taactttcta
     4021 tgctggagtt gttgtttctg cttttgcctg tagccgctgc ctatggctgg tatatgggcc
     4081 gcagaagtgc gcaacaaaac aagcaagatg aagccaaccg cttgtcgcgt gattacgtag
     4141 cgggggttaa cttcctgctt agtaatcaac aggataaagc ggtagacctg tttctcgata
     4201 tgcttaaaga ggatacaggc accgttgaag cccaccttac gctcggaaac ctgttccgtt
     4261 cgcgtggcga agttgatcgc gctattcgca tccatcagac cctaatggaa agcgcctcgc
     4321 tgacctatga acagcgtctg ttggcgattc aacaactggg gcgtgattac atggccgccg
     4381 ggttatacga ccgcgcggaa gacatgttca atcagctgac cgatgaaact gacttccgca
     4441 ttggcgcgct gcaacagttg ctacaaatct accaggctac cagtgagtgg cagaaagcaa
     4501 ttgatgttgc cgaacgcctg gtgaagctgg gtaaagataa acagcgcgtc gaaattgccc
     4561 atttctactg tgagttagcc ctgcagcata tggccagcga cgatctcgat cgtgccatga
     4621 ccttgctaaa aaaaggggcg gtggcagata aaaacagcgc ccgcgtatcc atcatgatgg
     4681 gacgcgtgtt tatggcgaaa ggagaatacg ccaaagccgt cgaaagtttg caacgcgtca
     4741 tatcccagga cagagaactg gtcagcgaaa cgctggaaat gttgcaaacc tgctatcagc
     4801 agttgggtaa aactgccgaa tgggcagaat tcctgcaacg cgcggtggaa gagaacaccg
     4861 gtgccgatgc tgaattgatg cttgctgata tcatcgaagc gcgcgacggt agtgaggctg
     4921 cacaggtcta tattacacgc cagcttcagc gtcatccgac catgcgtgta ttccataagc
     4981 taatggatta ccacttaaat gaagcggaag aagggcgcgc caaagagagt ctgatggtgc
     5041 tgcgtgacat ggttggcgag aaggtgcgta gtaagcctcg ttatagctgc cagaaatgtg
     5101 gttttaccgc atacaccctc tactggcatt gtccgtcttg tcgtgcctgg tcaactatta
     5161 aaccgattcg cggtcttgat ggcctgtaat ttttaaaaaa atccgacttt agttacaaca
     5221 tactagttat tgatgtccca ttgtgctccg gcaatgacgg agcagaaaaa gcctgctagg
     5281 ggaacaatcg caactgttaa ttttttattt ccacgggtag aatgctcgcc gtttacctgt
     5341 ttcgcgccac ttccggtgcc catcatcaag aaggtctggt catgacgtta actgcttcat
     5401 cttcttcccg cgctgttacg aattctcctg tggttgttgc ccttgattat cataattgtg
     5461 atgacgcgct gtcctttgtc gacaagatcg acccacgcga ttgtcgtctg aaggtcggta
     5521 aagagatgtt tacattgttt gggccacagt ttgtgcgcga acttcaacag cgtggttttg
     5581 atatctttct tgacctgaaa ttccacgata tccccaacac tgcagcgcac gctgtcgctg
     5641 ctgcagctga cttaggcgtg tggatggtga atgttcatgc ctccggtggg gcgcgtatga
     5701 tgaccgcagc gcgtgaggca ctggttccgt ttggcaaaga tgcaccgctt ttgattgctg
     5761 tgacagtgtt gaccagcatg gaagccagcg acctggccga tcttggcgtg acactgtcac
     5821 ctgcagatta tgcagaacgt ctggcagcac tgacgcaaaa atgtggcctt gatggtgtgg
     5881 tgtgttctgc tcaggaagct gtgcgcttta aacaggtatt cggtcaggag ttcaaactgg
     5941 ttacaccggg catccgtccg caggggagtg aagctggtga ccagcgccgc attatgacgc
     6001 cagaacaggc gttggcggct ggtgttgatt atatggtgat tggtcgcccg gtaacgcaat
     6061 cggtagatcc agcgcagaca ctgaaagcga tcaacgcctc tttacagcgg agtgcatgat
     6121 gagtgattcc aacagccgtc tggtctactc aacggaaacc gggcgtattg atgagcccaa
     6181 agcggcccca gtacgcccta aaggcgatgg agcggtgcgt attcagcgtc agaccagtgg
     6241 acgtaaaggt aagggcgttt gcctgattac cggtatcgat ctcgatgatg ccgaactgac
     6301 aaaacttgca gcggaactga agaaaaaatg cggctgcggc ggagcagtta aagatggagt
     6361 tattgaaatc cagggcgata agcgtgattt attaaaatca ctacttgaag cgaaagggat
     6421 gaaggtaaaa ctcgcaggcg gttaacataa aaagccacgg atatatccgt ggctttcgaa
     6481 tattttactg tgtgtattat tcagttattt cgtactgaac agaa
//
LOCUS       DADLZM010000175         6490 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLZM010000175 DADLZM010000000
VERSION     DADLZM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.177"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..892)
                     /locus_tag="JNY73_003678"
     CDS             complement(<1..892)
                     /locus_tag="JNY73_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY8049527.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGS"
     gene            952..1230
                     /locus_tag="JNY73_003679"
     CDS             952..1230
                     /locus_tag="JNY73_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049528.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="JNY73_003680"
     CDS             complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="JNY73_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY8049529.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="JNY73_003681"
     CDS             complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="JNY73_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY8049530.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3384..3716
                     /gene="hdeA"
                     /locus_tag="JNY73_003682"
     CDS             3384..3716
                     /gene="hdeA"
                     /locus_tag="JNY73_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY8049531.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3832..4158
                     /gene="hdeB"
                     /locus_tag="JNY73_003683"
     CDS             3832..4158
                     /gene="hdeB"
                     /locus_tag="JNY73_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY8049532.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4222..4868
                     /locus_tag="JNY73_003684"
                     /pseudo
     CDS             4222..4868
                     /locus_tag="JNY73_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4910..5440)
                     /locus_tag="JNY73_003685"
     CDS             complement(4910..5440)
                     /locus_tag="JNY73_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8049533.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5596..6162)
                     /gene="slp"
                     /locus_tag="JNY73_003686"
     CDS             complement(5596..6162)
                     /gene="slp"
                     /locus_tag="JNY73_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY8049534.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
BASE COUNT         1890 a         1340 c         1388 g         1872 t
ORIGIN      
        1 ggctaccttc atccactaat gccgtgacat acatgccagg cagcaagtca ccatttgggt
       61 tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc atcaaccgtc gggtcggaga
      121 atttcagcgt gccggtctgg ctgtaacgtt taccattttc cagattgagc tgtactggcg
      181 tactgccctg aacctgtttg atttgcccac tggcgacctc ttctttcatg cgtaagaaat
      241 cttgcaccga ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg
      301 aatctgcctg attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc
      361 ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg
      421 ctttggcgac ggtgacattg gcttctgctt cattcagctg ggtgcgcgcg gtgtcgtaat
      481 cctgacggct aacgtagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg
      541 cattgctggc ggtagagagc gctttcgcca gcgagccttt ggcggagttt agctcggcct
      601 gtaaaggtgc aggatcaatc tgatacagcg aatcgctctg gttcacttta tcgccttcga
      661 taaagttgcg tttaatgata ataccgccca cctggggacg tatctcggca gcttcataag
      721 gaacggttct accgggcaat tcgctcaaca cattgaccga actaggggag agtgtgacga
      781 caccgacctc aggcgtcatg gcggcggcgt tttccgccga tttgtcatcg caggcggtga
      841 gcatcgcgcc gcagaataac aacggtatta acagctttct tcttctgttc attttagtcc
      901 ctgaaaattc ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat
      961 ttacagacca gcggcatagt ggggttgtca gccagtaagg tgggatacag gcacagtgat
     1021 cgacatggtg aggtcaacga caaaaaaaat gttgccgttc tgccaacagt tcctgccagc
     1081 attcgggcaa cgaagggttc tactggtgga tacacatacc aggggaatca agtcgtttta
     1141 gcctctcctt tagtaatcac cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc
     1201 agccatcaat ttcagttgct tatgtcctga ctaaaaataa gatgtgatac ccagggtgac
     1261 gatgtcgctc atacgtctta gtttcagctt gtacataatg ttgaatttct gaatctttaa
     1321 tgtttgttgc gaaatattga gcgtcgacgt gatattgctt tcatttttat ttttcagaat
     1381 cagttgcata attttgaact gattatgtga aaaacataat tgtgaattct tatggggcaa
     1441 gtgtttacca taagtaatct caagaagatt tttgatactt tctttgcggc taacaaaata
     1501 aataggcgct ttagctttta gtttactgat gtgataaggt gccaaaacaa taattctctc
     1561 ggcatctaat ttctccagaa atttaatcgc ttcttcatca aggatatgat tgtgataggt
     1621 atcaatgata accttgaagg gtttattgcc tacttttttg atctctgaca gggaattgat
     1681 ttttatcatt tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt
     1741 cgtcatgaga aaaatcataa cttgctcctt agccgttatt gtttcgaaca tgtcatcctt
     1801 ttcattattt acatccttgt ccgaatcgtt gttcaatata gtaaacgcct ataagaactt
     1861 tgcccttcgt gaactaatac gtctattaac actaatcaat acttcagatt tatccatcac
     1921 gcactccata tattgatcct aagtaagatt cctggttgtt atcagcttgt aaatagtaaa
     1981 caaaaagtta acgtttgcgg attactgccc aagaataagt tatcaataac cagtttgtga
     2041 tctctgaaga atattactaa agttaaaatg taatccgatt taaatatcga gtctccttgt
     2101 ttcgacttaa gctggcaatt ggattgccag ctttctttta acgcattgaa attacctgag
     2161 taaataacgc atttatgtct tcaggttttt atatagcgtc tggagacacg gatatttatg
     2221 caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata
     2281 gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc
     2341 tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa
     2401 aaaaattaga tgaaattcag taggttgaaa taatcactag caggtaatta tttcaatgat
     2461 agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac
     2521 atcaagtgac tatgatccgg gtgacaaccg gggtaattat tgctgcttaa cgaacaaact
     2581 ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac
     2641 cagcgttaca gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag
     2701 cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat
     2761 caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat
     2821 gcccagctcc ggtgcacgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag
     2881 gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag
     2941 cgcaataccc gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac
     3001 gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat
     3061 tgctctgcga tgctttttca gcatctccag atcaaacttc aaaattgttg ccttatctat
     3121 atataacata gaaccaccct ataaaattaa gaagaaaatc ccctgctatc aatctatgcc
     3181 aaaaacgcgt ctaagaatgc agtcgattta ataaaaattt cctaattgca gtatctgatg
     3241 catctgtaac tcattgtatt gaaataaaaa tatctgattt tgatattttc catcaacatg
     3301 acatatacag aaaaccaggt tataacctca gtgtcgaaat tgattcgtga cggctctttc
     3361 actttatagt tgaggatatt acgatgaaaa aagtattagg cgttattctt ggtggtctgc
     3421 ttcttctgcc agttgtgagc aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac
     3481 cggtcaactc atggacctgt gaagatttcc tggctgtgga cgaatccttc cagccaactg
     3541 cagttggttt tgctgaagcg ctgaacaaca aagataaacc agaagatgcg gttttagatg
     3601 ttcagggtat tgcaaccgta accccagcta tcgttcaggc ttgtactcag gataaacaag
     3661 ccaactttaa agataaagtt aaaggcgaat gggacaaaat taagaaagat atgtaattcc
     3721 gggaatgcgt tacatcgtac ttccttgcat attgaacagg ccggaatatc ttctttaaaa
     3781 gcagctattc ctcctgttca tatataatct ctatattgaa tgggttacaa aatgaatatt
     3841 tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag cggctttgtc actggtgaac
     3901 gcacaatctg cgctggcagc caatgaatcc gctaaagata tgacctgcca ggaatttatt
     3961 gatctgaatc caaaagcaat gaccccggtt gcatggtgga tgctgcatga agaaacagta
     4021 tataaaggtg gcgataccgt tactttaaat gaaaccgatc tcactcaaat tcctaaagtg
     4081 atcgaatact gtaagaaaaa cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct
     4141 aatgacttgc cgaattaatg aggtacaagt aaaaaggagt agcaagttga gccatcttgc
     4201 tgctcctttt tgcattttta tatgacagca gaatttatta tacgtcttat actcgcggca
     4261 attgcctgtg gcgctattgg catggaaagg caaatgcgcg gcaaaggagc agggttacgc
     4321 acacatgtat taattggcat gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt
     4381 gctgacgtgc tgtctttaga tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg
     4441 gtgacgggcg tcgggtttat cggtgcaggt aacattctcg ttcgtaacca aaatattgta
     4501 ggtctgacga cggcagcgga tatctgggtg accgccgcat aggtatggtt attggcagcg
     4561 gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg gaagtcttcc
     4621 atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca ttagtgaacg
     4681 gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaact gatttagttt
     4741 cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt catgccacaa
     4801 cgttggtaga agacctgttg cgattattaa aaggtatggc aggggtgaaa ggcgtttcaa
     4861 ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt cacaccagat
     4921 aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg atcatcatga
     4981 tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc agaatatctt
     5041 cagttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc gaaagtgaca
     5101 cattcgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa tcaatgatag
     5161 tgatagtccg cggaacaaag gtcaccggta ctttccccag acagcgttta atattaaagg
     5221 gggagaaaac aatgacatgg acaggtttta atcgttcaat ttgagtgaga aaattagaat
     5281 gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc aacattacgt
     5341 cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg tttttcatcg
     5401 cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc tggtgcggac
     5461 attaacttta taatcagttt gccaaaggta attaacagga cagggcttat aataataata
     5521 gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag aaagcatctg
     5581 catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg cattggtgta
     5641 gtaaggcgca cccaagcccg gttccggcca gaatgcacca tagccgtaat cccacaggtt
     5701 ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca tattcacttc
     5761 caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa tggtaccgag
     5821 gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt gtctggcgag
     5881 cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca acggtaatac
     5941 agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct tcccaccaaa
     6001 gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa aacttttttg
     6061 aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg cggcaagcaa
     6121 aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat ccttatcaac
     6181 aaatattaaa accataatag atatttcttt atagttttca tctgattctg aggtgatatg
     6241 cctgatagta acgtaaacct gtctgatgtt tatatccctt ctgtttagtt caattccgtt
     6301 attttggggc gtggtatgag tatccatcgc ccaataatat aaccctttgt tttaaaatta
     6361 cataaaatct atttttattc aataattata aagtgctaat aatcaatatt atggtattat
     6421 attctgataa tgatttgata agcgaattct tttgccagat ggtatttcta tttccacccc
     6481 ataatttata
//
LOCUS       DADLZM010000176         6324 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLZM010000176 DADLZM010000000
VERSION     DADLZM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.178"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(337..627)
                     /locus_tag="JNY73_003687"
     CDS             complement(337..627)
                     /locus_tag="JNY73_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049535.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="JNY73_003688"
     tRNA            complement(1227..1302)
                     /locus_tag="JNY73_003688"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="JNY73_003689"
     CDS             complement(1408..2115)
                     /locus_tag="JNY73_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY8049536.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAIPLSAAWTAWFA"
     gene            2513..4648
                     /locus_tag="JNY73_003690"
     CDS             2513..4648
                     /locus_tag="JNY73_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY8049537.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADISPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /locus_tag="JNY73_003691"
     CDS             complement(4697..5953)
                     /locus_tag="JNY73_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY8049538.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..>6324)
                     /gene="mltC"
                     /locus_tag="JNY73_003692"
     CDS             complement(6155..>6324)
                     /gene="mltC"
                     /locus_tag="JNY73_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708734.1"
                     /note="Murein hydrolase C; membrane-bound; lytic;
                     catalyzes the cleavage of the beta-1,4-glycosidic bond
                     between N-acetylmuramic acid and N-acetylglucosamine
                     residues; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY8049539.1"
                     /translation="SVLRVFSNDKIQAANIINTMTPGDVYQTLTTRHPSAESRRYLYK
                     VNTAQKSYRRR"
BASE COUNT         1577 a         1500 c         1632 g         1615 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggtattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggcgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatatctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgc
//
LOCUS       DADLZM010000177         6241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLZM010000177 DADLZM010000000
VERSION     DADLZM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.179"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            7..312
                     /locus_tag="JNY73_003693"
     CDS             7..312
                     /locus_tag="JNY73_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8049540.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(388..852)
                     /locus_tag="JNY73_003694"
     CDS             complement(388..852)
                     /locus_tag="JNY73_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY8049541.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /locus_tag="JNY73_003695"
     CDS             complement(849..1724)
                     /locus_tag="JNY73_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY8049542.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEATASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="JNY73_003696"
     CDS             complement(1721..2410)
                     /locus_tag="JNY73_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY8049543.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JNY73_003697"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JNY73_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY8049544.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JNY73_003698"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JNY73_003698"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY8049545.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JNY73_003699"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JNY73_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY8049546.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1579 a         1691 c         1550 g         1421 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcagttgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgtggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcagcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatt
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 a
//
LOCUS       DADLZM010000178         5994 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLZM010000178 DADLZM010000000
VERSION     DADLZM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.180"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..268)
                     /locus_tag="JNY73_003700"
     CDS             complement(<1..268)
                     /locus_tag="JNY73_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY8049547.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /locus_tag="JNY73_003701"
     CDS             805..2706
                     /locus_tag="JNY73_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY8049548.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="JNY73_003702"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="JNY73_003702"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY8049549.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="JNY73_003703"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="JNY73_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY8049550.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="JNY73_003704"
     CDS             complement(4548..4757)
                     /locus_tag="JNY73_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049551.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="JNY73_003705"
     CDS             5041..5403
                     /locus_tag="JNY73_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY8049552.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1565 a         1454 c         1488 g         1487 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aagccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctc
//
LOCUS       DADLZM010000179         5857 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLZM010000179 DADLZM010000000
VERSION     DADLZM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.181"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            83..442
                     /locus_tag="JNY73_003706"
     CDS             83..442
                     /locus_tag="JNY73_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY8049553.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(562..741)
                     /locus_tag="JNY73_003707"
     CDS             complement(562..741)
                     /locus_tag="JNY73_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY8049554.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            815..2176
                     /gene="pabB"
                     /locus_tag="JNY73_003708"
     CDS             815..2176
                     /gene="pabB"
                     /locus_tag="JNY73_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY8049555.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2180..2758
                     /locus_tag="JNY73_003709"
     CDS             2180..2758
                     /locus_tag="JNY73_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY8049556.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHTGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2942..4306
                     /gene="sdaA"
                     /locus_tag="JNY73_003710"
     CDS             2942..4306
                     /gene="sdaA"
                     /locus_tag="JNY73_003710"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY8049557.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4437..>5857
                     /locus_tag="JNY73_003711"
     CDS             4437..>5857
                     /locus_tag="JNY73_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8049558.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPEATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVA"
BASE COUNT         1424 a         1464 c         1470 g         1499 t
ORIGIN      
        1 cattatctga acataaaaca ctatcagtaa gttggagtca ttaccaactc gtgccatatc
       61 gttataatcc ctttgatatt agatgcaaat taaggtcata tatagcctta tcgataacat
      121 ggttaatttt aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg
      181 tgccatggat gtatcagcgc caacaccaag ttcgatgaat gaaagcacgg cgaagggaat
      241 ctttaaatat ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc
      301 tgaccaggaa ggctggaacc ctgggttcac ggaaaaaatg gttggatggg caaaaaaaat
      361 ggagacaggt gaacgctctg tgattaaaaa tcctgaatac ttttcaacat atatgcagga
      421 agaactaaaa gcactggtct gagttaaatt tatatcagca taaatgggtc aggacgcttt
      481 taatcacata taaaaagcgt cctttttctc acaatcaaat gatcatcggc ttctataacg
      541 aaatcgatac ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattagctc
      601 gccggagtgg ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctactcat
      661 cccttgtgcc atcagttcct ggatccgctc gacagctttt tgctgctgtt catgggtgag
      721 tgaaggtaaa cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat
      781 gctacccggc acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat
      841 tactttaccc tggcgtcagg acgccgctga attttatttc tcccgcttaa gccacctgcc
      901 gtgggcgatg cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt
      961 ggtcgccgat ccgatttgca ctttaaccac tttaggtaaa gaaaccgttg ttagtgaaag
     1021 cgaaaaacgc acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg
     1081 cgcagacatt cgcccaacgc ataacgaaga tttgccattt cagggcggcg cactggggtt
     1141 gtttggctac gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat
     1201 cgttctgccg gatatggcag tgggtatcta cgattgggcg ctcattgtcg accaccagcg
     1261 tcatacagtt tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag
     1321 ccagcaattc tcgccgcagg aagatttcac gctcacttcc gactggcaat ccaatatgac
     1381 ccacgagcag tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg
     1441 ctatcaggtg aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc
     1501 attccttcag cttaatcagg ccaaccgcgc gccatttagc gcttttttac gtcttgaaca
     1561 gggtgcaatt ttaagccttt cgccagagcg gtttattctt tgtgataata gtgaaatcca
     1621 gacccgcccg attaaaggca cgctaccacg cctgcccgat cctcaggaag atagcaaaca
     1681 agcagaaaaa ctggcgaact cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt
     1741 aatgcgtaat gatatcggtc gtgttgccgt agccggttcg gtaaaagtac cagagctctt
     1801 cgtggtggaa cccttccctg ccgtgcatca tctggtcagc actataacgg cgcgactacc
     1861 agaacagtta cacgccagcg atctgctgcg cgcagctttt cctggtggct caataaccgg
     1921 ggctccgaaa gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgtaatgc
     1981 ctggtgcggc agcattggct atttgagctt ttgcggcaac atggatacca gcattactat
     2041 ccgcacgctg actgccatta acggacaaat atactgctct gcgggcggtg gaattgtcgc
     2101 cgatagccag gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg
     2161 ccaactggag aagtaaaacg tggaataccg tagcctgacg cttgatgatt ttttatctcg
     2221 ctttcaactt ttgcgcccgc aaattaaccg ggaaacccta aatcatcgtc aggctgccgt
     2281 gttaatcccc atcgtccgtc gaccacaacc ggggttgttg ctgactcagc gttcaattca
     2341 tctgcgtaaa cacactggac aagtggcatt ccctggaggt gcagtcgatg acacggacgc
     2401 atcagttatc gccgccgcgc tgcgcgaagc tgaagaagag gtcgctatac cgccttccgc
     2461 cgttgaagtt atcggcgtgc tgccgcccgt cgatagcgta actggctatc aggtaacccc
     2521 agtggtcggc attatcccgc ccgatctgcc gtatcgcgcc agtgaagatg aagtctcggc
     2581 ggtgtttgaa atgccgctcg cccaggcatt acatctgggt cgttatcacc ctttagatat
     2641 ctaccgccgt ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg
     2701 gggaatgacc gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga acccctgact
     2761 atacttatct ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat
     2821 cgcggttttt gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt
     2881 acactatgcg ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat
     2941 cgtgattagt ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt
     3001 agggcctatg aaggcgggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga
     3061 tagcgttact cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca
     3121 ccacaccgat atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat
     3181 cgacagtatt cccggtttta ttcgcgacgt aaaagagcgc gaacgtctgc tgctggcaca
     3241 gggacggcat gaagtggatt tcccgcgcga caacgggatg cgttttcata acggcaacct
     3301 gccgctgcat gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag
     3361 caaaacttat tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga
     3421 tgctgccaac gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta
     3481 ctgtaatgaa accggctatt cgctgtctgg tctcgctatg cagaacgaac tggcgctgca
     3541 cagcaagaaa gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat
     3601 cgatcgcggg atgaacaccg aaggtgtact gccaggcccg ctgcgcgtgc cacgtcgtgc
     3661 gtctgccctg cgccggatgt tagtttccag cgataaactg tctaacgatc cgatgaatgt
     3721 cattgactgg gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg
     3781 tgtggtaact gcgccaacca acggtgcctg cggtatcgtt ccggcagtgc tggcttacta
     3841 tgaccacttt attgaatcgg tcagcccaga catctatacc cgttacttta tggcagcggg
     3901 cgcgattggt gcattgtata aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca
     3961 gggcgaagtg ggtgttgcct gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg
     4021 tagcccggaa caggtttgcg tggcggcgga aattggcatg gaacacaacc ttggtttaac
     4081 ctgcgacccg gttgcagggc aggttcaggt gccgtgcatt gagcgtaatg ccattgcctc
     4141 tgtgaaggcg attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc
     4201 gctggataag gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ccaaataccg
     4261 cgaaacctca cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttactcgc
     4321 ccatctgcaa cggatgggcg aatttatacc cgctttctcg tctgctgtaa tattccccac
     4381 tacacttcca ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc
     4441 aaaaagcaca acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt
     4501 gcgcactcgt tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa
     4561 atcaacaacg ggtagtacaa ttcgccaatc acgctgtaga ggaattagat aaagtactgc
     4621 ttcctctaca ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg
     4681 cccatttgcc attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg
     4741 tgcaagacgg cacactttat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg
     4801 acattctggc tgaacttcct gcaccgcaac cacttttacg cctgacgatc gaccgtgccc
     4861 tgattaaagg cagtccggtt ttgattcaat ggacgcctgc agcaggcagt agcaatgctg
     4921 gggtcataga gatgattaac atcgacttac tgacggcaat gctccttgag ccacaactgc
     4981 cgcaaatcag tagcgccagc ctgacggtgg acaaacggca tttgctctat ggtaatgggc
     5041 tggtagattc ccttccgcaa cctgaagaca atgaaaacta tcaggtttct tcgcaaagct
     5101 ttccttttac cattaacgta aatggtccgg aggctacggc gctggcatgg cactatcttc
     5161 caacacaatt accgctggcg gtgctgctaa gtttactggt gggctatatc gcctggctgg
     5221 cgaccgctta ccggatgagc ttttcccgcg aaattaatct gggcctggcg caacatgagt
     5281 tcgaattgtt ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga
     5341 ttttgctgcg ctggaataat ccgcgtcagg gctggatttc accggatgtg tttattccta
     5401 tcgcggaaga acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc
     5461 gtcagcgcca tgttttcccg atgagtagtc agtttcatgt tggcattaac gtcgcaccca
     5521 gccattttcg ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc
     5581 cgattcagca actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattatc
     5641 ggattgcccg cgagctgcat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg
     5701 gcaacagctc gttttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt
     5761 catttaccgc agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg
     5821 cgctggggca aagactgaat attgaactgg tggcgga
//
LOCUS       DADLZM010000180         5805 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.182, whole genome shotgun sequence.
ACCESSION   DADLZM010000180 DADLZM010000000
VERSION     DADLZM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.182"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JNY73_003712"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JNY73_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY8049559.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JNY73_003713"
     CDS             578..1027
                     /locus_tag="JNY73_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8049560.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1260)
                     /locus_tag="JNY73_003714"
                     /pseudo
     CDS             complement(1028..1260)
                     /locus_tag="JNY73_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1346..1519)
                     /locus_tag="JNY73_003715"
     CDS             complement(1346..1519)
                     /locus_tag="JNY73_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY8049561.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1714..1809
                     /gene="yncL"
                     /locus_tag="JNY73_003716"
     CDS             1714..1809
                     /gene="yncL"
                     /locus_tag="JNY73_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY8049562.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2211..3020)
                     /locus_tag="JNY73_003717"
     CDS             complement(2211..3020)
                     /locus_tag="JNY73_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY8049563.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNY73_003718"
                     /pseudo
     CDS             complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNY73_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4677..5471)
                     /locus_tag="JNY73_003719"
     CDS             complement(4677..5471)
                     /locus_tag="JNY73_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8049564.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..>5805)
                     /locus_tag="JNY73_003720"
     CDS             complement(5461..>5805)
                     /locus_tag="JNY73_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8049565.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1470 c         1289 g         1524 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagccg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcgttggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg tgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggccgctt ccatatcttc
     1081 tttatacttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg
     1141 atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc
     1201 aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat
     1261 actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata agtctgcgtg
     1321 gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac
     1381 tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt
     1441 gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca
     1501 tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc gcgcaaatat
     1561 gcgccatttt ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag
     1621 caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta
     1681 tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta
     1741 ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg
     1801 tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac
     1861 agtacgcaag atcttctcat actctccaag tttgcaacat tatccttaat atttacttct
     1921 ttttgctggc atctacattg aaatgagtag aaaatatccc cctactgtcg tctatacaaa
     1981 cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa tactttatac
     2041 gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc
     2101 gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc ccattgaaat
     2161 tgcaaacccc gccctggtta tcttgcccat gtatcatgca aaacagataa ttataggcct
     2221 ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt tccatttgat
     2281 atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt attatccgga
     2341 agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat aacatttgaa
     2401 tcaaacctaa atctgattat ttttgcagga actcctgcaa ctatggcata tgggggcaca
     2461 tctttggtaa caacggaatt ggcagcaata acagcaccat cacctattgc gatgccacct
     2521 ttcaatacaa catcatttcc tatccagaca tcatttccta taatcggatt ttcaggtttg
     2581 gtaatgaatg gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa cttatcaaat
     2641 tcaccgttat aagtcatcat gtgagtagta aaacgattca aaggatgatt tccacccatt
     2701 atttttacat ttgatgcaat actacaaaat cgacctatac gtatattccc tgaaaaatgg
     2761 caggtcggaa atgaaaaagc gccgacactg gagaaatttt tcacaggcat tgttgaatat
     2821 tcttcagcaa taacattatc ttcaattgtt atcttgtcac cttttgagaa aagaaataaa
     2881 ttcctttctt tatacgactt cttcccctta tgatttaaaa agattttatt atccatacaa
     2941 aactcaaagt gttttttcgt ccatctgaag gtaagggttt tatttttatc ttccgtgaat
     3001 ttgtacaatt gaactttcat tgttaatact tctacttata aattattagt ttaataacga
     3061 tactctaaag ccttatgcta atcaactgtg acaggatgaa aaatcgccat caaacattta
     3121 gcctaatttt tacgccagtt attaatgtaa tcattctaat acttaaccgc cattaaactc
     3181 agaacctaaa acgatagtgg ccgtcctata acagccacta acatctaact taatgtttaa
     3241 ccatgacgtg gcggacgacg gtgtaatcct ccagcccata aagtgacata tccttgccgt
     3301 aaccagaaag tttctgccca ccgtgcggca tttcacttac cagcatgaaa tgggtattga
     3361 cccaggtaca accatattgc agccgtgcgc tgacgcgatg cgccctgccc acatctttcg
     3421 tccataccga agatgcaagt ccgtactggc tgtcattcgc ccagttcacc acctgttctt
     3481 cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac ctctttttgc acgatggcat
     3541 cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta atagccatta cccttgcgct
     3601 tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc
     3661 tgacgcgttc gagatgcgcc agcgagctta aaggtccaag ctccgtagac tcgtcatccg
     3721 gcgcaccaga ttttaacgtt gccaccgcag gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaaaata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatgc ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 accagttctc cgttaatcag taacttatgt tgcatagatt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 tagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DADLZM010000181         5753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLZM010000181 DADLZM010000000
VERSION     DADLZM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.183"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            337..1395
                     /gene="rfaQ"
                     /locus_tag="JNY73_003721"
     CDS             337..1395
                     /gene="rfaQ"
                     /locus_tag="JNY73_003721"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY8049566.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1392..2516
                     /locus_tag="JNY73_003722"
     CDS             1392..2516
                     /locus_tag="JNY73_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY8049567.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2509..3306
                     /gene="rfaP"
                     /locus_tag="JNY73_003723"
     CDS             2509..3306
                     /gene="rfaP"
                     /locus_tag="JNY73_003723"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY8049568.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3349..4356
                     /gene="waaO"
                     /locus_tag="JNY73_003724"
                     /gene_synonym="rfaI"
     CDS             3349..4356
                     /gene="waaO"
                     /locus_tag="JNY73_003724"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY8049569.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            4382..5089
                     /gene="rfaY"
                     /locus_tag="JNY73_003725"
     CDS             4382..5089
                     /gene="rfaY"
                     /locus_tag="JNY73_003725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY8049570.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            5114..>5753
                     /locus_tag="JNY73_003726"
     CDS             5114..>5753
                     /locus_tag="JNY73_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY8049571.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKL"
BASE COUNT         1741 a         1058 c         1242 g         1712 t
ORIGIN      
        1 tgacttaaat agcaatttac ccgccaattc ttgctgaaca tctatacttt ttttgtattt
       61 ttatgcgatt cattgatttc tgctgtacgt gcaggaagga atcgcagagc aacagtgaaa
      121 gaatatggtt gctaatgatt ttatttgatt gatttcttta ttgaaaatca acaatgtgct
      181 taaaaatcat tcgggtcgga ttgccgcgaa agaagtgata cactagcgcg tcaatatcag
      241 tgcggtatca gtattcaggt agctgttgag cctggggcgg tagcgtgctt tttctgcttc
      301 accttaatcg gataatctca acaaaaagag ttacttgtgg ataagccatt tcgaagaatt
      361 ttgctcatta agatgcgttt tcatggggat atgttattaa ctactcccgt cattagttct
      421 ctgaaaaaaa attaccctga cgcaaaaatc gatgtgctgc tttatcagga caccatcccg
      481 atcctgtctg aaaatccaga gattaacgcg ctctacggca taaaaaataa aaaagcaaaa
      541 gcctcagaaa aaattgccaa cttttttcat ctcatcaagg tattacgtgc caataagtat
      601 gaccttatcg tcaatcttac cgatcaatgg atggttgcta tactggttcg cttattaaat
      661 gctcgtgtga aaatttccca ggattatcat catcggcagt ctgctttttg gcgtaaaagt
      721 ttcacccatt tggtgccgtt gcagggtgga aatgtggtgg aaagtaactt atccgtgctg
      781 acaccattgg gacttgattc gttggtgaag cagacaacca tgagttaccc gcctgcaagc
      841 tggaaacgta tgcgtcgcga acttgatcac gctggtgttg gacaaaatta tgtggttatc
      901 caacctacgg cgcggcaaat cttcaaatgc tgggacaacg ccaagttttc cgctgtgatt
      961 gatgccttac atgctcgtgg ttatgaagtc gttctgacgt ccggcccaga taaagacgat
     1021 ctggcctgcg tcaatgaaat tgcgcaggga tgccagacgc caccagtaac ggcgctggct
     1081 ggaaaggtga ccttcccgga acttggtgcg ttaatcgatc atgcgcagct gtttattggc
     1141 gttgattccg caccggcgca tattgccgct gcagttaata cgccgctgat atcgctgttt
     1201 ggcgcaacag atcatatttt ctggcgtccc tggtcaaata acatgattca attctgggcg
     1261 ggagattacc gggaaatgcc aacgcgcgat cagcgtgacc gaaatgagat gtatctttcg
     1321 gttattccgg cggcagatgt cattgctgct gtcgataaat tactgccctc ctccacgaca
     1381 ggtacgtcgt tatgatcgtt gctttttgtt tatataaata ttttcccttt ggcggtttgc
     1441 agcgcgattt tatgcgtatt gctcagacag tcgccgcccg aggtcatcat gttcgggttt
     1501 atacccagtc gtgggaaggc gaatgccctg atgtatttga actgatcaaa gtgccggtta
     1561 aatcgcatac caatcacggg cgcaatgcgg agtattttgc ctgggtgcaa aaacatttac
     1621 gcgaacatcc cgtcgataaa gtcgttggat tcaacaaaat gccggggctg gacgtttatt
     1681 atgccgctga tgtttgttat gccgagaaag tagcgcagga aaaaggcttt ttctatcgcc
     1741 tgacgtcacg ttatcgccat tatgccgcct ttgagcgggc aaccttcgaa cagggcaagc
     1801 cgacacagct gctgatgctg acagataagc aaatcgccga tttccagaaa cattatcaga
     1861 ctgaagcgga gcgttttcat attctgccac cggggattta tcctgatcgt aaatatagcc
     1921 agcagccagc caatagccgt gaaatcttcc gtaagaagaa tggaataacc gaacaacaat
     1981 atttattgtt gcaggtcggt tcagacttca cgcgtaaagg tgtcgatcgt tccattgaag
     2041 cacttgcttc gttaccggat tcgctgcgcc acaacacatt gctatatgtt gttgggcagg
     2101 ataaaccgcg aaaatttgag gcactggcag aaaaacgcgg cgtgcgcagt aatgttcact
     2161 tcttctcggg gcgcaacgat gtctcggaat taatggcggc ggcggattta ttactgcatc
     2221 ctgcctacca ggaagcggcg ggaattgtgc tgctggaagc gattactgca ggattaccgg
     2281 tactaacaac tgccgtttgt ggctatgcgc attatattgt cgacgctaat tgcggcgagg
     2341 ctattgctga gccattccgc caggaaacat tgaatgagat tttacgcaaa gcgttaacgc
     2401 aatcttcatt gcgccaggct tgggcggaaa atgcgcgaca ttatgctgat acacaagatt
     2461 tatacagtct gccagagaaa gcggcggata tcataacggg tggtctggat ggttgaactt
     2521 aaagagccgt ttgccacgtt atggcgcggc aaagatcctt ttgaggaagt taaaaccttg
     2581 cagggtgagg tatttcgtga actggaaact cgccgtactc tgcgctttga aatggcggga
     2641 aaaagctatt ttctcaaatg gcatcgcggc acgaccctga aagagataat caaaaattta
     2701 ctctcattgc ggatgccagt attaggcgca gaccgcgaat ggaatgcgat tcatcgactg
     2761 cgggatgtcg gcgttgatac tatgtatggg gtggcattcg gcgaaaaagg catgaatccg
     2821 ctgaccagaa cctcgtttat tattaccgaa gatctgacac caaccatcag tctggaagat
     2881 tactgtgctg actgggcgac caacccacca gatgttcgcg taaagcgtat gcttattaag
     2941 cgtgtcgcga cgatggtgcg cgatatgcat gctgcgggca ttaaccatcg tgactgttat
     3001 atctgtcatt tcctgctgca cttgcctttt tccggtaagg aagaggagtt aaaaatttcg
     3061 gtaattgacc tacaccgggc gcagcttcgc acgcgcgttc caggtcgttg gcgcgataaa
     3121 gatcttatag ggctttattt ttcttcaatg aatattggcc tgactcagcg ggatatctgg
     3181 cgctttatga aggtgtattt tgccgccccg cttaaagaca ttctcaagca ggaacaagga
     3241 ctgctgtcgc aagcagaagc aaaagccaca aaaatcaggg aaagaacgat tcgaaaatcg
     3301 ttgtaacccg taacttattt ttgccaaaat tttggataca gaataaatat gtctcaactc
     3361 aatgatagtg acatcatcct ttttgagtat aattttcatt atcaaaatat aagatctaaa
     3421 aatactcttg atatagcatt tggtattgac agaaattttc tttttggatg tggtgtagcc
     3481 atcgcatcta ttctattaaa caatagagaa atctcttgtg aatttcatgt tttcacagat
     3541 tatatcagtg ataaagacaa attatatttt tctgatttag caaaacaata taattcaaga
     3601 attaatattt atgttatcaa ttgtgataag ctgaagtcat taccaagcac gaaaaactgg
     3661 acttacgcaa catattttcg atttataatt gcagattatt tctatcataa acatgaaaaa
     3721 atactatatc ttgatgcaga tattgcttgc aagggtagta ttaaagaact cttagattat
     3781 caattttcta ctaatgaaat tgccgcagtt gtagctgaaa gagatattga atggtggcaa
     3841 aatcgagcct cggtattaac tacaccacag ttagcttctg gatatttcaa tgctggtttt
     3901 ttactgataa atattgatga gtggaatcta aataacattt cgtcaaaagc tattgaaatg
     3961 ttgcgtgacc cagattgggt aagtaaaatc acccaccttg atcaagatgt actgaatgta
     4021 ttattgaatg gtaaagtgaa gtttatttcg gagaaatata atacccgata tagtattaac
     4081 tatgaattaa aagacaaagt tgataatcca gtcaatgatg acaccgtgtt tatacactat
     4141 gtgggaccta caaaaccctg gcatgagtgg gctgattatc cggtttcacg tagttttttg
     4201 atcgccaaag cagcttctcc gtggagtaaa gaagatttac ttaaacctgt aaatagcaat
     4261 cagtatcggt attgtgcaaa acataaattt aaacaaaagc attatatggc aggcattttt
     4321 aattatttaa agtattataa agaaaaatgc ttctaaagat ttaaggttaa aataattcat
     4381 tatgatatat aataaaacga taaatgggtt gaaagttttc ataaaagata atgatccctt
     4441 ttatgagcaa gttctaaatg actttttaac ttgcagggtt aaaacattaa aagtttttcg
     4501 ttctattgat gatactaaag tcattcttat tgacactgct cgtggacctc ttgtacttaa
     4561 agtttatgcg ccaaagcata aaatgaccga acgtttctta aaatcttgca taaaaaaaga
     4621 ttattatgaa aatttaattt atcaaaccga tcgggtacgt ggtgaaggca tccaatcaat
     4681 taatgactat tttttgctag ctgaacgtaa aacattaaat ttcgctcatt attatattat
     4741 gctcattgag tatattgaag gtgtcgggtt aaatgagtat ttggaaattt ctgaagatct
     4801 gaaggatcaa ttatcagaat cgataaaaga gttgcatcag catggaatgg tgtctggcga
     4861 tcctcataaa ggaaatttta tagtatccga aaaaggttta agactaattg acctttcagg
     4921 taaaaaaaca accgctgttt taaaagcaaa agatcgcatt gatcttgaac ggcactataa
     4981 tataaaaaat gaattgaaag actttggcta tacatatctt atattcaaaa agaaaattaa
     5041 aaaagtaatc agggatgtaa aagtcaaact aggattgaag agcaaataat gattatgatg
     5101 aagggtaatt aaattggatt ttaaacatct tactcaattt aaagatataa ttgaactgga
     5161 caagcgcccc gttaaacttg atgaacggga aacgtttaat gtctcatggg gtattgatga
     5221 gaactaccag gttggggctg cgatttcaat tgcttcaatt cttgaaaaga ataaacaaaa
     5281 caaatttacc tttcacataa tcgctgatta cttagacaaa gagtatattg aattattatc
     5341 acaattagca acgaagtatc aaacagtaat taaattatat catattgatt ctgagccatt
     5401 gaaggcgcta cctcaatcaa atatctggcc agtatctatt tattatcgtt tgctttcatt
     5461 tgattatttt tctgcgcgat tggattcatt attatatctt gatgctgata tcgtctgtaa
     5521 gggttcattg aacgagttaa tagcattaga gtttaaagat gaatatgggg cagtggtaat
     5581 tgatgtagat gctatgcaaa gtaaaagcgc tgagcgtttg tgtaatgagg attttaacgg
     5641 tagctatttt aactctggtg taatgtatat taatttacgg gaatggttaa aacaaagact
     5701 aacggaaaaa ttctttgatc tattatcaga tgagtcaatt ataaaaaaat taa
//
LOCUS       DADLZM010000182         5735 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLZM010000182 DADLZM010000000
VERSION     DADLZM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.184"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            55..1515
                     /locus_tag="JNY73_003727"
     CDS             55..1515
                     /locus_tag="JNY73_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY8049572.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVAEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="JNY73_003728"
     CDS             complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="JNY73_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY8049573.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1932..2643)
                     /locus_tag="JNY73_003729"
                     /pseudo
     CDS             complement(1932..2643)
                     /locus_tag="JNY73_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(2732..3478)
                     /gene="ydfG"
                     /locus_tag="JNY73_003730"
     CDS             complement(2732..3478)
                     /gene="ydfG"
                     /locus_tag="JNY73_003730"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY8049574.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3615..5660
                     /gene="dcp"
                     /locus_tag="JNY73_003731"
     CDS             3615..5660
                     /gene="dcp"
                     /locus_tag="JNY73_003731"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY8049575.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1435 a         1363 c         1482 g         1455 t
ORIGIN      
        1 tttattcata taacaaaaca tatagcttgc catatttaaa tttaaggaat tgttatggga
       61 aataatttgt tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca
      121 agaattgttc atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat
      181 attcttgcta cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc
      241 gaacagcaaa ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg
      301 gccgatgcgt ggacggctcg cgtcgttggc gtcgttaaaa aagccttgca cgtacagatg
      361 gatggcttag aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg
      421 acaatcaccg aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac
      481 ccgatggtcg ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt
      541 gttgaggcgc tggctcgccg taaagcggca ggacttcccg catttaccgt catgtcatgt
      601 gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta cgcgcaagcc
      661 gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg
      721 gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca acttaccggt
      781 gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat cgaagataac
      841 tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag tgatgtgctg
      901 ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcatatctg
      961 ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca ttatcgtcat
     1021 gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat
     1081 ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt acgtcatcga
     1141 acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt
     1201 cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg
     1261 atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag tgacccactg
     1321 ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca
     1381 ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt gtttactgca
     1441 aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa
     1501 tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac ttagttcatg
     1561 ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt
     1621 tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca
     1681 gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga
     1741 tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc acgtaacggg
     1801 cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt
     1861 ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg gatgggatga
     1921 gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat ctggcgcacg
     1981 gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt
     2041 ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg tggagaaaca
     2101 tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc aatggtatcc
     2161 cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc atcaagttca
     2221 aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc
     2281 agttggtagc actggctttc ggtaatcatg ctcgcactgg ctttcggtaa tcatgctcgc
     2341 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     2401 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     2461 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     2521 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     2581 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     2641 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     2701 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     2761 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     2821 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     2881 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     2941 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     3001 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     3061 gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     3121 ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca atcatcgttt
     3181 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     3241 ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc tcttcaatag
     3301 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta
     3361 actcctgcaa ccgctcctgg cgacgcccag tggcgataac tttatgccct tgttgaataa
     3421 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     3481 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     3541 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     3601 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     3661 tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     3721 tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     3781 acaatactat tctggcactg gaacaaagcg gagaattaat tacccgcgtt accagcgtct
     3841 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     3901 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     3961 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     4021 tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa gctgataaag
     4081 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcggt
     4141 tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     4201 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     4261 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc
     4321 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     4381 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     4441 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     4501 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat
     4561 tagcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg cagccgtggg
     4621 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     4681 tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg accgcgaatc
     4741 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc
     4801 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     4861 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     4921 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc gctgccggtg
     4981 agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt ggtcatacgc
     5041 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     5101 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     5161 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     5221 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     5281 ttctcgatat gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt
     5341 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     5401 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     5461 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     5521 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     5581 tggaacgcct gcatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac
     5641 atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     5701 ctggttatag cagtaattgc agcatgttac gacaa
//
LOCUS       DADLZM010000183         5689 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLZM010000183 DADLZM010000000
VERSION     DADLZM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.185"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..87)
                     /locus_tag="JNY73_003732"
     CDS             complement(<1..87)
                     /locus_tag="JNY73_003732"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY8049576.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNY73_003733"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNY73_003733"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY8049577.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSSPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGEGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="JNY73_003734"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="JNY73_003734"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY8049578.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5467..>5689
                     /locus_tag="JNY73_003735"
     CDS             5467..>5689
                     /locus_tag="JNY73_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8049579.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYNSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLK"
BASE COUNT         1752 a         1286 c          976 g         1675 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctctc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact gaaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggctg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gagggggaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aattttatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccggaaatc ctggagacta aactccctga gaaagaggta
     5461 aacaggatga ctaaaaatac acgtttttcc cctgaagtcc gtcaacgggc agttcgtatg
     5521 gttctggaaa gtcagggcga atataactca caatgggcag cgatttgttc cattgcccca
     5581 aagattggct gtacgccgga gactctgcgt gtctgggttc gccagcatga gcgggatacc
     5641 gggggcggtg atggagggct caccaccgct gaacgtcagc gtctgaaag
//
LOCUS       DADLZM010000184         5627 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLZM010000184 DADLZM010000000
VERSION     DADLZM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.187"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(88..507)
                     /locus_tag="JNY73_003736"
                     /pseudo
     CDS             complement(88..507)
                     /locus_tag="JNY73_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            557..1411
                     /gene="ltrA"
                     /locus_tag="JNY73_003737"
                     /pseudo
     CDS             557..1411
                     /gene="ltrA"
                     /locus_tag="JNY73_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1819..1998
                     /locus_tag="JNY73_003738"
                     /pseudo
     CDS             1819..1998
                     /locus_tag="JNY73_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2028..2510)
                     /locus_tag="JNY73_003739"
                     /pseudo
     CDS             complement(2028..2510)
                     /locus_tag="JNY73_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2766..3746)
                     /locus_tag="JNY73_003740"
     CDS             complement(2766..3746)
                     /locus_tag="JNY73_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY8049580.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3746..4945)
                     /locus_tag="JNY73_003741"
     CDS             complement(3746..4945)
                     /locus_tag="JNY73_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY8049581.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5267..>5627)
                     /locus_tag="JNY73_003742"
     CDS             complement(5267..>5627)
                     /locus_tag="JNY73_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8049582.1"
                     /translation="TFHPSLTQHWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTP
                     FCIGMVTSDDWGSYAREVPKEKHLTGKIFTQRIERNNRTLRTRIKRLARKTICFSRSV
                     EIHEKVIGSFIEKHMFY"
BASE COUNT         1550 a         1278 c         1336 g         1463 t
ORIGIN      
        1 tcgtgatgac cgtaacgccg gttcggaaat tgcagcataa gttatccatg ctctgaggtg
       61 ggtggaggtg ggccctccgg tcagggttta caggagcact gaccaaacga cccggtgctg
      121 cttcattcta aggaatgagg tggtgagcga ctcgggaaaa ggcagagatc tactgtcacg
      181 tggggttcgg gaagctgaac gaaagtaaac tgtccgatga agcatcgaaa tcttagatga
      241 tgccaaaatg gggccgtaca gccgtcccct gataagcgca gaagacacct gcttactgac
      301 tgcgcggcat ccggtgtgaa ggcagcagga tcccgggcca gaccatctca gggaacgtga
      361 gaacctgttt ccggcacgta tcgggaaaca ggggcggacc cattcgtagt agtgatgaaa
      421 gatctgtcat gggtctggag cgaagggatg gggatcacat tcattgcaga agacacaacc
      481 caaaagggga tgacgtaact gatgcaacga aaatcatttg aaataccaaa agcactggtc
      541 tgggcatcat atctggatgt gcgtcgcaac aagggagccc cccggatgcg acgggcagac
      601 tttgaaaatg ttcgaccagc aacgtgatgg caatctatac aagatctgga accggctatg
      661 ttcgggaaca tggtttccgc caccagtgct ggaaaaacgg atcccgaagt cgaatggcaa
      721 ggaacgcatt ctggggatcc caacagtatc cgatcgaatt gctcagggag cgataaaact
      781 tttcatggaa gaaaagcttg acccgatttt ccatgctgat tcatatggtt accgaccagg
      841 caaatcagct catgacgctc tgaaacaatg tgccatccgg tgctggcgtt atagctggat
      901 actggaagtg gacatcagtg cgtttttcga ccatgtgaga catgatctgg tgctcaaagc
      961 tctggaacac cacgggatgc ctaaatgggt catcctgtac tgtcgacgct ggatggaagc
     1021 gccaatgcag agttgtgaaa atggagaatt aataacccga acacgaggca ctccgcaggg
     1081 cggggtcatt agtccgctac ttgctaactt gttccttcac tatgcatttg atttgtggat
     1141 ggaaagagaa tatcgggggg taccgtttga gaggtacgct gacgatattg tagtacattg
     1201 ttcacgaatg agtgatgtaa caaggctaaa gaacaggtta tcggagcgct tctcggaagt
     1261 cgggctggtc ctgaatgcag ggaaaacgaa tatcgcctac attgacacgt ttaaaaggag
     1321 aaacgtcgca acgagtttca cctttctcgg atatgacttc aaagtgcgta cgctgaagaa
     1381 tttcaaaggc gaacggtacc gaaaatgcat gccgggtgcg tcaaatgcag caatgctgca
     1441 aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag agtttgctgg
     1501 attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac ggaaagttct
     1561 ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg ctcaagtgga
     1621 tgcagtctaa atacagactt tcgaaccgga aggctcagcg aaagctgacg ctggtaagga
     1681 aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat gagtgatcaa
     1741 gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga gtgaaattct
     1801 ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc ggtgttgtac
     1861 tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct gcgttacgtc
     1921 attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctggaaagtt
     1981 gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat
     2041 tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt
     2101 atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ctggacgtcc
     2161 tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct
     2221 attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt
     2281 gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat
     2341 gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcacctgcca gttaagcagt
     2401 tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga
     2461 taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc
     2521 ttacttttgt ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg
     2581 ggaagactat ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa
     2641 gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa
     2701 aagcaaaatt tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg
     2761 aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg atctaactta
     2821 tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt
     2881 tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc
     2941 tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt
     3001 ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata
     3061 ttcttcacaa cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc
     3121 agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct
     3181 ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt
     3241 tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt
     3301 tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa
     3361 acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct
     3421 gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat
     3481 ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg
     3541 gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca
     3601 tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga
     3661 actggagccg atatttcttc agtattattc aggatatttg tattcagcgt acggccaata
     3721 gtcggtcggt gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa
     3781 aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc
     3841 caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa
     3901 aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca
     3961 ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg
     4021 cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag
     4081 ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga
     4141 tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa
     4201 ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt
     4261 tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt
     4321 cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt
     4381 ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca
     4441 gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa
     4501 ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta
     4561 cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat
     4621 atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa
     4681 tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat
     4741 agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa
     4801 gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct
     4861 tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct
     4921 tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta
     4981 caaaaaaaac tgcattagca gggtaatgca tgcaagtaca tattgtacat gcttatatta
     5041 aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg
     5101 cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg
     5161 acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg
     5221 acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt
     5281 gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga
     5341 ttgttttacg tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgttcaatac
     5401 gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc
     5461 cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc
     5521 gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt
     5581 tgtacgcata ccagagccag tgttgtgtca acgacggatg aaaagtg
//
LOCUS       DADLZM010000185         5599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLZM010000185 DADLZM010000000
VERSION     DADLZM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.188"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..410
                     /locus_tag="JNY73_003743"
     CDS             <1..410
                     /locus_tag="JNY73_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8049583.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /locus_tag="JNY73_003744"
                     /pseudo
     CDS             442..1056
                     /locus_tag="JNY73_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNY73_003745"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNY73_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY8049584.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSIVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1379 a         1402 c         1329 g         1489 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actatacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DADLZM010000186         5597 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLZM010000186 DADLZM010000000
VERSION     DADLZM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.189"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            47..682
                     /locus_tag="JNY73_003746"
                     /pseudo
     CDS             47..682
                     /locus_tag="JNY73_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(736..1341)
                     /gene="azoR"
                     /locus_tag="JNY73_003747"
     CDS             complement(736..1341)
                     /gene="azoR"
                     /locus_tag="JNY73_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY8049585.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1542..5444
                     /gene="hrpA"
                     /locus_tag="JNY73_003748"
     CDS             1542..5444
                     /gene="hrpA"
                     /locus_tag="JNY73_003748"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY8049586.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNVRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1385 a         1369 c         1554 g         1289 t
ORIGIN      
        1 tatttcagga aggagtgtcc gttaaactca ggctacctca gtcgtaggcg cgtgcattgt
       61 gttgatggag ctaatgatga tggttcagtt atggtggtca gtctggttat gttggccagt
      121 attatcgcta ctcattgttg gccgtgggtg cggtgggctt ggcgcgataa caacagggaa
      181 agatagtcag gggaaactcc cgcccgccgt ttactggctg acattgccct ggcgcatcgg
      241 gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag ccggtgagca aaatgactgc
      301 tggtgtttat ttaagggcgt ttccacgaca tattccggca cagaatgcgg ttctggacgt
      361 cacctttgaa ttccctcgtg gacgagcgac aaaagatcga ctctatttct gtgtaccgat
      421 gttggatctg gtggttccgg aagaggggga gctccgacag gccgtggcga tgctggaaac
      481 attacgcgaa gagcaaggca gcgttctggt ccattgcgca ttgggattat cgcgcagtgc
      541 gctggtagtg gcggcatggc tgttatgtta cggacactgt aaaacagttg atgaagcgat
      601 tagctttatt cgagccagac gctcgcatat tgtgcttaag gaagagcaca aagcgatgtt
      661 gaaattatgg gaaaacaggt aagtggattg agatgtggac tgaatatcta cagtccacat
      721 caagaccgtg tccggttatg cagcaacaat gctgtcgatg gctgcttttg cgtcagactg
      781 tgctttcgct gccatttccg gaccgtatgc gatcccttcg gcgaagacaa atttcacatc
      841 ggtaatgccg ataaagccga ggaacgtgga cagatacggc gtcaccaggt ccgttggtcc
      901 atctttgtgg atcccaccgc ggctggtaat aacgatggct tttttacccg ttaccagacc
      961 ttccggaccg ttctcggtat agcggaaagt aacgcctgcg cgtgcaacca ggtcaaaata
     1021 gtttttcaac tgagttgaga tgttgaagtt atacatcggt gccgcaataa cgataacgtc
     1081 gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc agagcttcct gctgacgcgg
     1141 agtcagcggc gcatcgctcg gacgcagagc gccaaccagt tcgccatcca gtaccggaat
     1201 cggatttgca gctaggtcgc gaacggtgat ttcatcagcg gagtgctttt cgcgccattg
     1261 ttcaacaaaa taatcggaca actgattaga ctgagagtac cctgccagga tgctggattt
     1321 aagaactaat accttgctca tggtgtttcc ttatagatgt ttgaatggac gatgccccgt
     1381 tgcttgctga cactttattc acaatcctgc cacagagata gcgcaataaa tcgaagtcta
     1441 tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt ggtactctat atctatcatt
     1501 taaaagaaaa ttaatcaggc agactgctgc ccactaacgt tatgacagaa caacaaaaat
     1561 tgacctttac tgccttgcaa cagcggctgg attcgctgat gctgcgcgac agactgcgtt
     1621 tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc tgatgcacaa caggccattt
     1681 tccaggagat ggcgaaagag attgaccagg cggcagggaa agtcctgctg cgtgaagcgg
     1741 cacgaccgga aatcacttat cctgacaatt taccggttag tcagaaaaaa caggacatac
     1801 tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg ggaaaccggc tccggtaaaa
     1861 cgactcagct accgaaaatc tgtatggagc tggggcgcgg tattaaaggg ctgatcggtc
     1921 atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa ccgtattgcg gaagagctga
     1981 aaacggagcc gggcggttgc atcggttaca aagtgcgttt cagcgatcac gtaagtgata
     2041 acacgatggt caagctgatg accgacggga tcctgctggc ggagatccag caagaccgcc
     2101 tgctgatgca gtacgacacc atcattattg acgaagcgca cgaacgcagc ctgaatatcg
     2161 attttctgct cggctatttg aaagagttgc tgccgcggcg tcctgaccta aaaatcatta
     2221 tcacttccgc gactatcgac ccggaacgct tttcgcgcca ctttaataat gcgccgatta
     2281 ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta tcgcccgatt gttgaagaag
     2341 ccgatgacac cgagcgcgat cagttgcagg cgatttttga cgccgtagac gaactaagcc
     2401 aggaaagccc cggcgacatt ctgatcttta tgagcggcga gcgggaaatt cgcgataccg
     2461 ccgatgcgct gaacaagctg aacgtacgcc ataccgaaat cttgccgctt tatgcgcggc
     2521 tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag cggacggtgc attgtgctgg
     2581 cgaccaacgt cgcggaaacc tcgctgaccg taccggggat taaatacgtc atcgaccccg
     2641 gtacagcgcg tatcagccgc tacagctatc gcaccaaagt gcagcgtttg ccgattgaac
     2701 ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg tggtcgtgtg tccgaaggga
     2761 tctgtattcg tctctattcc gaagacgatt tcctctcgcg cccggagttt accgatccgg
     2821 agattctgcg taccaacctg gcctcggtta ttttgcagat gaccgcgctg gggctgggcg
     2881 atatcgctgc gttcccgttt gtcgaagcac cggataaacg caatatccag gacggcgtgc
     2941 gtctgctcga agaactgggc gcgatcacca cggatgaaca ggccagcgcc tataaactga
     3001 cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc acgtctggcg cgtatggtgc
     3061 tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat tatcacgtcc gcgctctcca
     3121 tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc atcggacgaa aaacatcgtc
     3181 gcttccacga caaagaatcc gacttcctcg cgtttgtaaa tctgtggaat tatcttggcg
     3241 agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct gtgtcgtacc gattacctca
     3301 actatctgcg cgtgcgcgaa tggcaggata tctacaccca gttgcgccag gtggtgaaag
     3361 aacttggcat tccggtcaac agcgaaccgg cggagtatcg tgaaattcac atcgccttac
     3421 tgaccggttt gctttcccat atcggcatga aagatgccga taaacaggaa tataccggtg
     3481 cacgtaacgc gcgtttctcc atcttccccg gttctggttt attcaaaaag ccgccgaaat
     3541 gggtaatggt ggcggaactg gtagaaacca gccgcctgtg ggggcgcatt gctgcgcgta
     3601 tcgacccgga atgggtagaa cccgttgctc agcatttgat taaacgcacc tacagcgaac
     3661 cgcactggga acgggcgcag ggcgcggtga tggcaacgga aaaagtcact gtttatggtt
     3721 tgccgattgt tgctgcgcgc aaggtcaact acagccagat cgattcggcg ttatgtcgtg
     3781 aactctttat tcgccacgct ctggtggaag gtgactggca gacgcgtcac gcattcttcc
     3841 gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga acacaaatca cgtcgccgcg
     3901 atattctggt tgatgacgaa acgctgtttg agttctacga ccagcgcatc agccacgatg
     3961 taatctccgc tcgtcacttc gatagctggt ggaaaaaagt cagccgcgaa acgcctgatt
     4021 tgctcaactt tgaaaagagc atgttgatca aagaaggggc ggaaaaaatc agcaagctgg
     4081 attacccgaa cttctggcat cagggcaatc tcaagctgcg tttgagctat cagtttgagc
     4141 caggcgcgga cgcggacggt gtgaccgtac atattccgct gccgttactt aaccaggttg
     4201 aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg cgaattggtg attgctctga
     4261 ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc tgcaccaaac tacgccgaag
     4321 cgtttttagg ccgcgtcaca ccgctggagt taccgttgct cgacagcctt gagcgcgagt
     4381 tacggcggat gaccggcgtt accgttgacc gcgaagactg gcactgggat caggtgcccg
     4441 atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa caagaagcta aaagaagggc
     4501 gctcgctaca agatctgaaa gatgcgctga aaggcaaagt gcaggaaacg ctgtccgcgg
     4561 tggcggatga cggtatcgag cagagcggct tacatatctg gagttttggt cagctgccgg
     4621 aaagttacga acagaagcgt ggcaactata aagtgaaggc gtggccggcg ctggtggatg
     4681 aacgcgacag cgtggcgatc aaactgtttg ataacccgct ggagcaaaaa caggcaatgt
     4741 ggaatggtct tcgccgtcta ctgctgctaa atatttcctc gccgatcaag tatttacatg
     4801 agaagttacc aaacaaagcc aagctggggc tgtactttaa cccgtatggc aaagtgctgg
     4861 agctgatcga cgactgtatc tcctgcggtg tggatcaact gatcgacgcc aatgatggcc
     4921 cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa agtgcgcgcc gaactgaatg
     4981 acacggtggt ggatattgcg aagcaggtcg agcaaatcct cactgcggtg ttcaatatca
     5041 acaaacgtct gaaagggcgg gtggatatga ccatggcact ggggctttct gacattaaag
     5101 cgcagatggg cgggttggta tatcgcggtt ttgtcactgg taacggcttc aaacggctgg
     5161 gcgacacgct gcgatatttg caggcgattg aaaaacgact ggaaaaactg gcggttgatc
     5221 cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt ccagcaggcg tggcagcaat
     5281 ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga cgtgaaagag atccgttgga
     5341 tgatagaaga gctgcgcgtt agttacttcg ctcaacaact tggtacgcct tatccgattt
     5401 cagataagcg tattttgcag gcgatggagc agattagcgg ttaaccctgc tatttgccta
     5461 ataaatctaa aacccggtaa gcatttagcg ccgggttttt tattaattct aaaacggtaa
     5521 ggacaaaaat tcaggaattc agaaaaatac aattctcagc tgcaagatga ataatgttta
     5581 tctacagcat ttcctta
//
LOCUS       DADLZM010000187         5583 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.190, whole genome shotgun sequence.
ACCESSION   DADLZM010000187 DADLZM010000000
VERSION     DADLZM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.190"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            29..244
                     /locus_tag="JNY73_003749"
     CDS             29..244
                     /locus_tag="JNY73_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8049587.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="JNY73_003750"
     CDS             237..623
                     /locus_tag="JNY73_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY8049588.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="JNY73_003751"
     CDS             631..1317
                     /locus_tag="JNY73_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8049589.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="JNY73_003752"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="JNY73_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY8049590.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="JNY73_003753"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="JNY73_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY8049591.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="JNY73_003754"
     CDS             complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="JNY73_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY8049592.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3999..4409
                     /locus_tag="JNY73_003755"
                     /pseudo
     CDS             3999..4409
                     /locus_tag="JNY73_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4425..>5583)
                     /locus_tag="JNY73_003756"
     CDS             complement(4425..>5583)
                     /locus_tag="JNY73_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY8049593.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1543 a         1030 c         1215 g         1795 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat aaggttttta
     3601 ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc agaccaacca
     3661 tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg ctgcttataa
     3721 caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc tttggtcacc
     3781 aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc aacaaacaat
     3841 attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt
     3901 ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga
     3961 tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc
     4021 gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa
     4081 cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg
     4141 atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaaag
     4201 taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa gcggctgttg
     4261 agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa tttgattccc
     4321 aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt tattatcggc
     4381 gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa tttccccaaa
     4441 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
     4501 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
     4561 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
     4621 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
     4681 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
     4741 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
     4801 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
     4861 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
     4921 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
     4981 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
     5041 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
     5101 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
     5161 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
     5221 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
     5281 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
     5341 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
     5401 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
     5461 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
     5521 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
     5581 aat
//
LOCUS       DADLZM010000188         5538 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLZM010000188 DADLZM010000000
VERSION     DADLZM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.191"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JNY73_003757"
                     /pseudo
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JNY73_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JNY73_003758"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JNY73_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY8049594.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5538)
                     /locus_tag="JNY73_003759"
     CDS             complement(2659..>5538)
                     /locus_tag="JNY73_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY8049595.1"
                     /translation="SSTSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAV
                     QQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGS
                     EYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQT
                     RLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAG
                     ANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLV
                     MYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVV
                     VENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQF
                     SITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDS
                     TRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTT
                     KVLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTA
                     IIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLS
                     LAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGISYVNDF
                     LNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYN
                     GIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYA
                     ISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIG
                     LSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHG
                     AGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1464 a         1286 c         1302 g         1486 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgtag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gatgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catagcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtcttttata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaaga
//
LOCUS       DADLZM010000189         5531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLZM010000189 DADLZM010000000
VERSION     DADLZM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.192"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            100..1008
                     /locus_tag="JNY73_003760"
     CDS             100..1008
                     /locus_tag="JNY73_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY8049596.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNY73_003761"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNY73_003761"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY8049597.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /locus_tag="JNY73_003762"
     CDS             3323..4081
                     /locus_tag="JNY73_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8049598.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNY73_003763"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNY73_003763"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY8049599.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="JNY73_003764"
     CDS             complement(5343..>5531)
                     /locus_tag="JNY73_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049600.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1323 a         1484 c         1478 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tccacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag ccaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gataccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DADLZM010000190         5487 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLZM010000190 DADLZM010000000
VERSION     DADLZM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.193"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(97..699)
                     /gene="fimB"
                     /locus_tag="JNY73_003765"
                     /pseudo
     CDS             complement(97..699)
                     /gene="fimB"
                     /locus_tag="JNY73_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            2153..2869
                     /gene="nanC"
                     /locus_tag="JNY73_003766"
     CDS             2153..2869
                     /gene="nanC"
                     /locus_tag="JNY73_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY8049601.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2889..3995
                     /locus_tag="JNY73_003767"
     CDS             2889..3995
                     /locus_tag="JNY73_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY8049602.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4060..5040
                     /locus_tag="JNY73_003768"
     CDS             4060..5040
                     /locus_tag="JNY73_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY8049603.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTTLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1665 a         1142 c         1154 g         1526 t
ORIGIN      
        1 acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt aagaataatg
       61 tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcat gacgctgtcg
      121 tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg tataccagac
      181 agtatgacga atattgcgat gcccaagata atcctagata agtcgcgtat ctattcctat
      241 attcgccaaa gcaaaaccac acgaatagcg taacatgtgc ggatgaatct ccagtgacaa
      301 cccggcatta ccaccagaag tcgagataat atggtaaaac tgttgccgag aaagcggatt
      361 ccccttacgt gataaaaata cccactcgct ctcagcatgc ggataagaag tacggatact
      421 caaccagttt tttaaagcct gaacttcttt gttcaatagc gggtgcgttg ttgaaaagcc
      481 tttttttaat cgatgtatat atatacactt tgctttaaga tcaatatccg aaatcctcaa
      541 tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaaatca gacaataatt
      601 acgtgttgca tgaggcccgg tatttgctgc tttaaggagt gactcgattt cactatgggt
      661 aaggaagttc ctttttttgt tatcagcctt attcctcatc gttttccctt ataattacag
      721 acgcgcacta gctgtgctgg gttagcaata atccaacatt tttatcctga ttatgtttat
      781 aaaagcgaac gtttgcttaa taacttacgt aagtggacca gttcttctga gcgaacttaa
      841 atggagtagc gatagttact tataataact ttgccataca aacgccatgc tcttgcatgc
      901 tatgtacctt tatatattta tcaatctgat acgaatctaa taataaccct catcattaat
      961 gcaactaatc ttatctatat atcatgtgat atgttctgta acaagtaaaa tcaacataaa
     1021 aaattcagca aaatttaata tttgttcaac tagccgtctc aaatatgaca ttattagtta
     1081 actgcatttg ccacaaaatg gctatttcat ggaacgcagt aatttcttag tggctattat
     1141 catgctaatt cttagatccc tagcgattat tcctgcctgg ttcgtcagct taggccatga
     1201 gattgttaca ttccttttta tcctggagat atgggggaac gcaggcgacg ctttttcatc
     1261 tacatccgtt ggtaaatata gctttggtca ctacgactta agccttcctc aatcacagct
     1321 attcgccggg cgatacggca aacaaactaa gtgaaacatc atcctgaaaa caccaacatc
     1381 aacaagcctc tccagatcga catcaggagt gaccagttac aagccacaaa ccaagaaaca
     1441 tatttcatga tgagaattat gctcagcctg atggcgagta aacccttatt ttcaccactc
     1501 tcactgagtt agcttgcaaa tctttctttg tttagattct aaataaatta tttataaaac
     1561 atatagttaa aagaaaatct ttagtttttg aagaccaccg caagtgttcg tctggcttca
     1621 catggcatct tcttcttaga aaaagatcga catattttgt gacgcgaatt gcaaatctgg
     1681 ttttgttgta tggattgcgt gatttttgat ctggtataac aggtataaag atgcaccacg
     1741 atagtcaatg agactgggca tctcgtaatc tatggcaaac atcacttcag ttctttcaca
     1801 tcgggtgatg aaaatgcact tcagtctgaa aggaatatga aaatgagatc aacagacact
     1861 ctattttatg actctgggta aaatgggttg agtaagtgat ataacttacg aatattcaaa
     1921 tcaattaaaa atcagaagag attttatact caggtattta atctggatct ctgtttattt
     1981 aaataatgtt aaaagagaat tttcacagga gaccttatac aaaaaatata aaatacagtt
     2041 acccgttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat aaatgctaat
     2101 tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa
     2161 ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa tttctcaggc
     2221 tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgcctatg agactcgact
     2281 caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta atacctggaa
     2341 taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa
     2401 ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa cgcattttag
     2461 cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga caaaagatct
     2521 taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag acttatccgg
     2581 tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc atattaatag
     2641 tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaatgatt atcgctatgc
     2701 aaaccacaag aaatgggcga cggaaaatgc ctttgttcta caataccata tgacgcccga
     2761 tattacgcca tacatagaat atgactacct tgaccgccag ggtgtttaca acggcagaga
     2821 taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt agacaggaga
     2881 cagtcacaat gaataaaact ataatggcgc ttgctatcat gatggcttca tttgccgcaa
     2941 acgcgtctgt attaccggaa actcctgtgc catttaaaag tggtaccgga gcaattgata
     3001 acgacactgt ctacattggt ttaggtagcg caggtacagc atggtacaag ctagatacac
     3061 aggccaaaga taaaaaatgg actgcgttag ctgcattccc tggtggaccg agagagcaag
     3121 caacctctgc atttattgat ggcaatctgt atgtgtttgg cggcattggc aaaaacagcg
     3181 agggattgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc aatagctggg
     3241 ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca tgtgactttt gtacacaacg
     3301 gcaaggctta tgttactggc ggtgttaacc agaatatctt caatggctat tttgaagatc
     3361 tcaacgaggc tggaaaagat tcaaccgcta tagataaaat caacgcccac tattttgaca
     3421 aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc tcaacacagc
     3481 aatggagtta cgctggcgaa tcgccctggt acggaacggc tggtgcggcg gttgtgaata
     3541 aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga acggatgccg
     3601 tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgat cccgtctcat
     3661 caccagatgg cgtcgctggc ggttttgcgg ggataagcaa tgattctctt atatttgccg
     3721 gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac tatgcgcatg
     3781 aaggcctgaa aaaatcatat agcactgata ttcatctttg gcataacggg aaatgggata
     3841 aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg aataatagtt
     3901 tattaattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca gttttaattt
     3961 ctgtgaagga taataaagtc acagtacaaa actaacgctt cagggccccg gtaaaggggc
     4021 cctgctatca atttgtcatt tgtattacgc gaaaattata tgaacgcaat aatatcgccc
     4081 gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta tggcgaagga
     4141 ctgccattac cggacaggga agatgcgcct cattccagaa ttaaacaatt agcgagattt
     4201 gcgcatacgc atcccggagg cccgccatgt cactttaacg acattattcc actgactcac
     4261 tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac gaatcatcaa
     4321 acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact gcccttcatt
     4381 cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc ttttaccgcg
     4441 ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg tcgttgggga
     4501 acggatacta cgctgtacca ggatttagtc agcagaacgc gagccgcact ggcaaaaaat
     4561 ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg aatttgactt aatgaccagt
     4621 gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg tagggatcta
     4681 aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg cggcgatacc
     4741 acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg caattatcaa
     4801 aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga aagaggactg
     4861 acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg ttcagcgtac
     4921 cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac ggcagcccgt
     4981 cgggggatta tttctgacag atttgtagaa gcaattttgc agttttggcg cgaaaggtga
     5041 gcggggagtt tattcatggg ggatgtcctt gttcgttctc agagtagaca acaaaaaaca
     5101 aatgagatca aggtaatatc tcttcacttg cgggctcgaa cagcaggcaa ttgccaatat
     5161 aataatctgg cacaagggtg aaaaacgcgc accgcataaa ccgttgttat tgcttacaga
     5221 ttagccggat gccttaatag acatccgcgc cttttcgatt atggctcgga aatctacgaa
     5281 catctacaca gtttactgga acgttttggg ccacagcgtt cgcagcaccg tcctgatatg
     5341 ccgttctggc gattataagt cgacggattc tggcaaatag acgatgatga tatgcttaat
     5401 gatgttcaaa gaaattaggc cattattata ttattgcttg cattttcttt cattcctaaa
     5461 agcacccgcc attaatccca ggcgcat
//
LOCUS       DADLZM010000191         5392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLZM010000191 DADLZM010000000
VERSION     DADLZM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.194"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..446)
                     /locus_tag="JNY73_003769"
     CDS             complement(<1..446)
                     /locus_tag="JNY73_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY8049604.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /locus_tag="JNY73_003770"
     CDS             complement(501..1352)
                     /locus_tag="JNY73_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY8049605.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALSAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /locus_tag="JNY73_003771"
     CDS             complement(1365..2165)
                     /locus_tag="JNY73_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY8049606.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JNY73_003772"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JNY73_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY8049607.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="JNY73_003773"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="JNY73_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY8049608.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFFFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..5389)
                     /locus_tag="JNY73_003774"
     CDS             complement(5216..5389)
                     /locus_tag="JNY73_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rtn protein"
                     /protein_id="HAY8049609.1"
                     /translation="METQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPA
                     RHNGHITPIMSLR"
BASE COUNT         1311 a         1362 c         1373 g         1346 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcact cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttcttcttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg cc
//
LOCUS       DADLZM010000192         5337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLZM010000192 DADLZM010000000
VERSION     DADLZM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.195"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNY73_003775"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNY73_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY8049610.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JNY73_003776"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JNY73_003776"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY8049611.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JNY73_003777"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JNY73_003777"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY8049612.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JNY73_003778"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JNY73_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY8049613.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1456 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggt
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADLZM010000193         5294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLZM010000193 DADLZM010000000
VERSION     DADLZM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.197"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..583
                     /locus_tag="JNY73_003779"
                     /pseudo
     CDS             1..583
                     /locus_tag="JNY73_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            647..1573
                     /gene="glsB"
                     /locus_tag="JNY73_003780"
     CDS             647..1573
                     /gene="glsB"
                     /locus_tag="JNY73_003780"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY8049614.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1573..1932
                     /locus_tag="JNY73_003781"
     CDS             1573..1932
                     /locus_tag="JNY73_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY8049615.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2071..3489
                     /locus_tag="JNY73_003782"
     CDS             2071..3489
                     /locus_tag="JNY73_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY8049616.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3716..5167
                     /locus_tag="JNY73_003783"
     CDS             3716..5167
                     /locus_tag="JNY73_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY8049617.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1224 a         1234 c         1422 g         1414 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gcactgaaaa aatgcgtact actatccgcc
      241 aacggttctg gcgaatgtta ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc
      301 cgttgctgca atcaccattg cgaaagatgc agaacatgcg ctggaactgg ctaatgatag
      361 tgagttcggc ctttcagcga ccatttttac caccgacgaa acacaggcca gacagatggc
      421 ggcacgtctg gaatgcggtg gggtgtttat caatggttat tgtgccagcg acgcgcgagt
      481 ggcctttggt ggcgtgaaaa agagtggctt tggtcgtgag ctttcccatt tcggcttaca
      541 cgaattctgt aatatccaga cagtgtggaa agaccggatc tgacgcgaca tattcagctc
      601 tgatatactc gcaggtcttt tcagacctgc ggtccaggag tagaaagtgg cagtcgccat
      661 ggataatgca attttagaaa acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa
      721 agtcgcggat tatattccgg cgctggctac agtagacggt tcccgattgg ggattgctat
      781 ctgtaccgtt gacggacagc tttttcaggc cggagacgcg caagaacgtt tttccattca
      841 gtctatttcc aaagtgctga gtctcgttgt cgccatgcgt cattactccg aagaggaaat
      901 ctggcaacgc gtcggcaaag atccgtctgg atcaccgttc aattctttag tgcaactgga
      961 aatggaacag ggtattccgc gtaatccgtt cattaatgcc ggtgcgctgg tggtctgcga
     1021 tatgttgcaa gggcgactaa gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt
     1081 aagcggtgtg tctgatattt catacgatac ggtggtagcg cgttccgaat ttgaacattc
     1141 cgcgcgaaat gcggctatcg cttggctgat gaagtcgttt ggcaatttcc atcatgacgt
     1201 gacaaccgtt ctgcaaaact actttcatta ctgcgctctg aaaatgagct gtgtagagct
     1261 ggcccggacg tttgtctttc tggctaatca ggggaaagct attcatattg atgaacctgt
     1321 ggtgacgcca atgcaggcgc ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca
     1381 gaacgcgggg gagtttgcct ggcgggtggg actaccggcg aaatctggcg ttggtggcgg
     1441 tattgtggcg attgttccgc atgaaatggc catcgcagtc tggagtccgg aactggatga
     1501 tgcaggtaac tcgcttgcgg gtatcgccgt ccttgaacaa ttgacgaaac agttagggcg
     1561 ttcggtttat taatgcagtc tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt
     1621 cgctctcgct ttcgtctggg catgaaagag cgtcagtatt gcctggagaa aggcgcgcca
     1681 gtcatcgaac aacatgcggc ggattttgtc gctaaacgtc ttgctccggc attaccggct
     1741 aatgacggta agcaaccccc catgcgcggg catccggtgt ttatcgctca gcacgccacc
     1801 gcgacctgct gtcgcggttg tctggctaag tggcataaca ttccgcaggg tgtttcgtta
     1861 agcgaagaac aacaacgtta tatcgtggcg gtaatctatc actggctggt tgtacagatg
     1921 aatcagccat gagaattgcg gaaaacgata aaatagcatt cccagggtac ctttatttgc
     1981 cgccgctgtt agcgaacatg ctatccttta tgagaattta cttatcacgt tgagataatt
     2041 gccacccgca attgtatgga cagataatta atgcatgtac accccatttc aactttccgt
     2101 ttgttccagg aaggtcatct gctacgtaat agcatcgcta tttttgcgct aaccacgctg
     2161 ttctatttta ttggtgcaga gttacggctg gttcacgaac tttctctttt ctggccgctg
     2221 aatggcgtaa tggcgggggt gtttgcccgc tatgtctggc ttaatcgact gcattactat
     2281 gcgatcagtt atgtggcgat gctggtttat gatgccataa ccaccgaatg gggactggtt
     2341 tcactggtta tcaatttctc caatatgatg tttattgtta ccgtcgcctt actggtcgtg
     2401 cgggataagc gtctggggaa aaataagtat gagccagtaa gtgccttacg gctatttaat
     2461 tactgtctga ttgccgcatt attatgcgct attgtcgggg cgattggttc ggtcagtatt
     2521 gatagtctgg atttctggcc tttgcttgcg gactggttca gtgagcaatt ctcaacgggc
     2581 gtgttgatcg tgccttgtat gctgacgttg gctattcctg gagtgctgcc gcgctttaaa
     2641 gcagaacaga tgatgcccgc catcgcgctt attgtgtcgg ttattgcctc ggtagtcatt
     2701 ggcggagcgg ggagtctggc gttcccgctg cctgcattaa tctggtgtgc agtgcgctat
     2761 acgccgcagg taacatgtct gttgaccttt gtcaccggtg cggtggaaat cgtactggtg
     2821 gcaaattcgg tgattgatat ctcggtcggt tcgccgttct ccattccgga aatgttctcc
     2881 gcacgtctcg gtattgccac gatggcgata tgcccaatta tggtttcttt tagcgtggca
     2941 gcgatcaatt tgctaatgaa gcaagttgcg ctgcgagccg actttgattt tctgactcag
     3001 gtttactcac ggtccggtct ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa
     3061 catctgacgg tcatgctgct tgatatcgac tatttcaaaa gcattaacga taactatgga
     3121 catgaatgtg gcgataaagt gttaagcgtg tttgcccggc atattcagaa gattgtcggt
     3181 gataaggggc tggtggcgcg catgggcggc gaagaatttg ctgttgcagt gccgtcggtg
     3241 aatcctgtcg atggtctgct aatggcggaa aaaatccgta aaggcgttga actgcaacca
     3301 ttcacctggc aacaaaaaac gctttatctc acggtaagta ttggcgtcgg tagtggtcgc
     3361 gcatcgtacc gaacgctgac cgatgatttt aataaattga tggtcgaagc cgatacgtgt
     3421 ctgtatcgct cgaagaaaga tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa
     3481 gttgtctgac atctttattc attccgcgcg atttgaatgc aatgtgttct accactttat
     3541 gatccaggaa aaggcgtatt tcctcgatta aaccatgatc cgcgccacac ttttctcata
     3601 aaactgctca ttaatcatca ggagttgatc aaaacagggg ttcggcgtat taaagtttgc
     3661 taacctgtcg caccaattca aacaggttgt atgactaatc agaagggaac ccattgtgaa
     3721 aacactaaat cgtcgcgatt ttcccggtgc acagtatcca gaacgtatca ttcagtttgg
     3781 tgaaggtaac ttcctgcgcg cctttgttga ctggcaaatc gatctcctga atgagcacac
     3841 cgatctgaat tctggcgtgg tcgttgttcg tccgattgaa acttcattcc cgccgtcact
     3901 gagcacgcag gatggtctgt acaccaccat tatccgtggc ctgaatgaga agggggaagc
     3961 ggtcagcgac gcgcgtctga ttcgctcggt taatcgtgaa atcagcgtct acagtgaata
     4021 cgatgaattc ctgaaactgg cgcacaaccc ggaaatgcgc tttgttttct ctaacaccac
     4081 cgaagcgggt atcagctacc atgcgggcga taaattcgat gacgcgccag cggtaagcta
     4141 tccggcaaaa ctgacccgtc tgctgttcga acgctttagc catttcaacg gtgcgctgga
     4201 taaaggttgg atcatcattc cgtgtgagtt gattgactac aatggcgacg cgttgcgtga
     4261 actggtgctg cgctatgcgc aagagtgggc actgccagaa gcatttattc aatggctgga
     4321 tcaggctaac agcttctgtt ctacgctggt ggaccgtatc gttaccggtt atccgcgcga
     4381 tgaagtggct aaactggaag aagaactggg ttatcacgat ggtttcctcg acaccgctga
     4441 acacttttac ctgtttgtga ttcaggggcc gaaatcctta gcgactgagc tgcgcctgga
     4501 caaatatccg ctcaacgtgc tgattgttga cgatattaaa ccgtataaag aacgcaaagt
     4561 ggcaatcctc aacggtgcgc acaccgcgct ggtgccggtg gcgttccagg cagggctgga
     4621 taccgtaggt gaagcgatga acgatgctga aatttgcgca ttcgttgaaa aagccatcta
     4681 cgaagaaatt attccggtac tggatttgcc tcgtgatgaa ctggaatctt tcgccagtgc
     4741 agtcaccggg cgtttccgta acccgtacat taagcatcag ctgctgtcta tcgcgctcaa
     4801 cggtatgacc aagttccgca cccgcatcct gccgcagctg ctggcagggc agaaggcaaa
     4861 cggcacactt ccggcgcgcc ttactttcgc attagcggca ttgattgcgt tctatcgtgg
     4921 tgagcgtaac ggagaaacat atccggtaca ggacgatgca cactggctgg aacgttatca
     4981 gcaactctgg agccagcatc gtgaccgtgt tatcggtacc caggaactgg tagcgattgt
     5041 gttggcagaa aaagaccact gggagcagga cctgacgcaa gtgcctggtc tggtcgagca
     5101 ggttgctaat gacctggatg cgattcttga aaaagggatg cgcgaagctg tacgtccgtt
     5161 gtgctaataa caccgttact tctgaacccg gtttcgaccg ggtttcttat ttcactatat
     5221 tagttacaac atcctatatg cctttatcat taacaggtgt cacatgcagc gcctgttaat
     5281 gttgtaagct tgac
//
LOCUS       DADLZM010000194         5265 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.198, whole genome shotgun sequence.
ACCESSION   DADLZM010000194 DADLZM010000000
VERSION     DADLZM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.198"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..166
                     /locus_tag="JNY73_003784"
     CDS             <1..166
                     /locus_tag="JNY73_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049618.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            349..2529
                     /locus_tag="JNY73_003785"
     CDS             349..2529
                     /locus_tag="JNY73_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY8049619.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2670..3953)
                     /locus_tag="JNY73_003786"
     CDS             complement(2670..3953)
                     /locus_tag="JNY73_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY8049620.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4088..5158)
                     /locus_tag="JNY73_003787"
     CDS             complement(4088..5158)
                     /locus_tag="JNY73_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8049621.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKIFASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5136..>5265)
                     /locus_tag="JNY73_003788"
     CDS             complement(5136..>5265)
                     /locus_tag="JNY73_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY8049622.1"
                     /translation="FPPPVKDGREYLFHESAVKVDLNRPVTGSLLKRIRNGKKAKS"
BASE COUNT         1253 a         1290 c         1331 g         1391 t
ORIGIN      
        1 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
       61 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct
      121 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
      181 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
      241 cctggggatt cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt
      301 tattatcttt ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta
      361 tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc
      421 ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct
      481 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
      541 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg
      601 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
      661 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
      721 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggctggcgg gggcaaaatg
      781 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
      841 ggtggcggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc
      901 gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg
      961 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
     1021 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
     1081 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
     1141 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
     1201 gatggctgca tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac
     1261 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
     1321 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
     1381 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
     1441 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     1501 ttctcgctgg cggtttaccc gtcatcacag ccggtgttta tggatctagc caaaaaaggt
     1561 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc
     1621 tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac
     1681 ttcccgaacc gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgctgatg
     1741 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     1801 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     1861 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     1921 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     1981 atcctcgacg aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     2041 cgttcaaatc cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     2101 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     2161 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     2221 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     2281 gtcatcaact gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg
     2341 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     2401 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg
     2461 acggctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccat
     2521 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     2581 gctggcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     2641 cgccgcatcc ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg
     2701 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     2761 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     2821 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     2881 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
     2941 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
     3001 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
     3061 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
     3121 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
     3181 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg
     3241 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacact atcgcccagc
     3301 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
     3361 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
     3421 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
     3481 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
     3541 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc
     3601 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
     3661 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
     3721 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
     3781 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
     3841 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
     3901 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     3961 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     4021 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
     4081 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
     4141 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
     4201 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
     4261 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
     4321 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
     4381 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     4441 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     4501 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     4561 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     4621 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
     4681 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
     4741 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
     4801 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
     4861 aatttatgag tgtcttctgc ttgattcctc tgctggcgaa gattttttcg tagcgatcaa
     4921 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
     4981 tgtgtcctga aaataactca atgttggcct gtatagcttc agtgattgcg attcgcctgt
     5041 ctcggcctaa tccaaactct ttacccgtcc ttgggtccct gtagcagtaa tatccattgt
     5101 ttcttatata aaggttaggg ggtaaatccc ggcgctcatg acttcgcctt cttcccattt
     5161 ctgatcctct tcaaaaggct acctgttact ggtcgattta agtcaacctt taccgctgat
     5221 tcgtggaaca aatactctct tccatcctta accggaggag ggaat
//
LOCUS       DADLZM010000195         5151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLZM010000195 DADLZM010000000
VERSION     DADLZM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.199"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            190..447
                     /locus_tag="JNY73_003789"
                     /pseudo
     CDS             190..447
                     /locus_tag="JNY73_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNY73_003790"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNY73_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY8049623.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /locus_tag="JNY73_003791"
     CDS             complement(1599..2351)
                     /locus_tag="JNY73_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY8049624.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNY73_003792"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNY73_003792"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY8049625.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNLISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JNY73_003793"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JNY73_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3605..4420)
                     /locus_tag="JNY73_003794"
     CDS             complement(3605..4420)
                     /locus_tag="JNY73_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8049626.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            4517..>5151
                     /locus_tag="JNY73_003795"
     CDS             4517..>5151
                     /locus_tag="JNY73_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8049627.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1174 c         1225 g         1377 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgagat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtggcagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tctggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg acaggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagaagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctagcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DADLZM010000196         5144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLZM010000196 DADLZM010000000
VERSION     DADLZM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.200"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..253
                     /locus_tag="JNY73_003796"
                     /pseudo
     CDS             1..253
                     /locus_tag="JNY73_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="JNY73_003797"
     CDS             746..1492
                     /locus_tag="JNY73_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8049628.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="JNY73_003798"
     CDS             1507..1929
                     /locus_tag="JNY73_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY8049629.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="JNY73_003799"
     CDS             1987..2343
                     /locus_tag="JNY73_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049630.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="JNY73_003800"
     CDS             2436..2654
                     /locus_tag="JNY73_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY8049631.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="JNY73_003801"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="JNY73_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3683
                     /locus_tag="JNY73_003802"
     CDS             3018..3683
                     /locus_tag="JNY73_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049632.1"
                     /translation="MATITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSGEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3683..4048
                     /locus_tag="JNY73_003803"
     CDS             3683..4048
                     /locus_tag="JNY73_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8049633.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4446..4658
                     /locus_tag="JNY73_003804"
     CDS             4446..4658
                     /locus_tag="JNY73_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8049634.1"
                     /translation="MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            4895..>5144
                     /locus_tag="JNY73_003805"
     CDS             4895..>5144
                     /locus_tag="JNY73_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049635.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT         1407 a         1087 c         1421 g         1229 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
     4501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     4561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     4621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg gggagaaatc
     4681 cctcgccacc tctcatgtgt caggcatcct caacgcaccc gcactaaacc cgcttcggcg
     4741 ggttttttgt tgcgtgctga atacgcaggg tgaaaaataa ccatatattt gattatatac
     4801 acaacaaaaa ataaaagtca ttgtacttgc acattaaaca atcaaatata cggcgtgaaa
     4861 taaatatttt tcagattaat atttttgtct ctatgtggat ataaccgttt gtacttataa
     4921 acctggaggc atcgtggaaa aaataaagaa actatttagt agcaaatacg cagtcatacg
     4981 tcgtgatgac ctgtcagtta tagtcgaaat ggattacttc cctgaaaccc caaaatcaat
     5041 aatgtatcgt aatggtcgaa aggcaatttt tttaccgatg agggtaagtg acattatggg
     5101 aaatgataaa ctgctggatg aattgcgagt cagagcatcc tgtt
//
LOCUS       DADLZM010000197         5126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLZM010000197 DADLZM010000000
VERSION     DADLZM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.201"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..236
                     /locus_tag="JNY73_003806"
     CDS             <1..236
                     /locus_tag="JNY73_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049636.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="JNY73_003807"
                     /pseudo
     CDS             276..816
                     /locus_tag="JNY73_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /locus_tag="JNY73_003808"
     CDS             883..2301
                     /locus_tag="JNY73_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY8049637.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHVVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /locus_tag="JNY73_003809"
     CDS             2282..2752
                     /locus_tag="JNY73_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY8049638.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JNY73_003810"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JNY73_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY8049639.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGEIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="JNY73_003811"
     CDS             3834..4751
                     /locus_tag="JNY73_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY8049640.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAAWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="JNY73_003812"
     CDS             4830..5012
                     /locus_tag="JNY73_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY8049641.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1225 c         1464 g         1185 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtaag cctggcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg tggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatctcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagagcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gcgagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg ggcgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcggc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DADLZM010000198         5116 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLZM010000198 DADLZM010000000
VERSION     DADLZM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.202"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..366)
                     /locus_tag="JNY73_003813"
     CDS             complement(<1..366)
                     /locus_tag="JNY73_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049642.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            892..1866
                     /gene="hemB"
                     /locus_tag="JNY73_003814"
     CDS             892..1866
                     /gene="hemB"
                     /locus_tag="JNY73_003814"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY8049643.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="JNY73_003815"
                     /pseudo
     CDS             complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="JNY73_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="JNY73_003816"
     CDS             complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="JNY73_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY8049644.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLLLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="JNY73_003817"
     CDS             complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="JNY73_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY8049645.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="JNY73_003818"
     CDS             complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="JNY73_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8049646.1"
                     /translation="VPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTH
                     YSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTD
                     SEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENIS
                     KLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAIIDTAQFLKEQGKVPAVAN
                     DYSQYVTSRFVQ"
BASE COUNT         1243 a         1408 c         1273 g         1192 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa
      541 ttgataaaag gacgatattt cagcatgttg cagataatgt tatttttgat gcatacgcgt
      601 tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat
      661 tattcaattt atagcagcac aaccaaacgc caaaaaaaat aataaccaag agctataaat
      721 aaagattaaa aatattttca gtgaatatga aattattgct atttctatta caaagatgtc
      781 ctggtctctc tttcattctc ttcttgtcaa catcgcgaca actttcgtaa aacactcctg
      841 ccccgcttca ggtatactgt gcaccttaat tccaaaaaca tcaggcagac catgacagac
      901 ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag
      961 acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac
     1021 tacaaagccg ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc
     1081 gaaattgaac gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac
     1141 cataccgatg aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg
     1201 cgcatctgca agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa
     1261 tacacttctc acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact
     1321 ctggaaaatt taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc
     1381 ccttccgccg cgatggacgg ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc
     1441 tttaaagata cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggcccg
     1501 ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca
     1561 atgaaccgtc gcgaggcgat tcgtgagtca ctgctggatg aagcccaggg cgcagactgc
     1621 ctgatggtta aacctgctgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact
     1681 gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg
     1741 ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt
     1801 gcgggtgcgg atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg
     1861 cgttaatttt tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc
     1921 cctcacccta accctctccc caagggggcg agggaaccgt ccacactcgt atcaccccgc
     1981 ccgataaaac ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag
     2041 gtaatcggca ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg
     2101 ttgccagcgc caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa
     2161 caaggcttcg ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac
     2221 aaacagcgcc tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg
     2281 atttttcgcg accgcctcgc gccagcgttg atgttcctcc tcggttttgc ggtatttgta
     2341 ttccgggaat gatttacgga aatcatgctc cgcacgcagc cagctcagca gctggcggaa
     2401 gggaacagag agcgcctcat aggccgcaat accgctggtt cagagcgtat caccgccggt
     2461 cgaaggtaac tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac
     2521 atcggtatgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat
     2581 ctcgtcaacc ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg
     2641 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaata ccacctgatg
     2701 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc
     2761 ggcacccgaa atttgtgcgc cgatatgcgg ccccagcggg gtaatgctca gacgttcact
     2821 cattgtactt ctccatgcca gggcgtcaga cggcgctgta acgcgcgcag acccagttct
     2881 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga
     2941 aattcacccg ctgactgaac cataaatcct aaaccgcgtg tcgcggcaat cagctccgcc
     3001 gctaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
     3061 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
     3121 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccctg ccagcgccga catcgccacc
     3181 ggcgcaaaaa tcgctaaata gatcagtaag atcttcgagg tttcaccaat accaaaccag
     3241 atcaccatca gcagcaaata agccagcggt ggcaccggac gataaagttc gattaccgga
     3301 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
     3361 accaccgccg cgagtagcgc ctgcacaatg cgcgtcaggc tggctgccag atgctgccac
     3421 agcgtggcgt ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt
     3481 tgcggcggcg gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca
     3541 gtgagtaaaa ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc
     3601 catttcagcc gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
     3661 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
     3721 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
     3781 ccagcacacg gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt
     3841 cttctatatc gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca
     3901 gcagagtttg catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcatcgagta
     3961 ataacagctg gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccat
     4021 cggaaagctg ccagatatag cgtttttctg cgccttccag ccccactttt ttcagcatct
     4081 ggtgcgcgat ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca
     4141 cgttgtcctg tacattgcgc cacggtagca gcccttcatt ctgaaaaact acgccacgct
     4201 ctgctcccgg tccctcaata cgcttacccg ccagttgaat gctgccatgc tgataaggca
     4261 caaaaccggc aatcagattc agcagggtgg ttttaccgca gccggacggc cccagcacca
     4321 ccagtagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
     4381 aatcggcata gagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg
     4441 aggtaacgta ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa
     4501 actgcgcggt gtcgatgatt gctttgttca ccggtccggt cagttctgcc gtttgttgct
     4561 gcggcgtcag ataggtattt cccttcacca gccccggaac gtcaccttca ggcacgccgc
     4621 ttaaacgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtca gggttagcaa
     4681 tgtacggttg ctgtgcatcg atggcgcttt tggcgaacgc tttcacgacc tcaggatgtt
     4741 tctcggcaaa atctttgcgc accacccaga cgtccagcgt tggcgcaccc cactgcccga
     4801 cctgttcaga atcggtcagc accttgccgt ctttttccag ggcgttaacc gccggtgccc
     4861 agacataagc accatcaata tcaccccgct gccatgcggc gataatcgcg ggcggctgca
     4921 ggttcacaat ctccactagc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc
     4981 tgtagtgggt ggtggagata aacggtacgg caatgcgttt accaatcaaa tcttcgggtt
     5041 tgctgatagt tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga
     5101 agacttcaat cggcac
//
LOCUS       DADLZM010000199         5070 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLZM010000199 DADLZM010000000
VERSION     DADLZM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.203"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(295..543)
                     /locus_tag="JNY73_003819"
     CDS             complement(295..543)
                     /locus_tag="JNY73_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049647.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(656..922)
                     /gene="ariR"
                     /locus_tag="JNY73_003820"
     CDS             complement(656..922)
                     /gene="ariR"
                     /locus_tag="JNY73_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAY8049648.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(951..1223)
                     /locus_tag="JNY73_003821"
     CDS             complement(951..1223)
                     /locus_tag="JNY73_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAY8049649.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKLAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(1266..1502)
                     /locus_tag="JNY73_003822"
     CDS             complement(1266..1502)
                     /locus_tag="JNY73_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAY8049650.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            1816..3027
                     /gene="bluF"
                     /locus_tag="JNY73_003823"
     CDS             1816..3027
                     /gene="bluF"
                     /locus_tag="JNY73_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY8049651.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            3232..3963
                     /locus_tag="JNY73_003824"
     CDS             3232..3963
                     /locus_tag="JNY73_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY8049652.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            4184..4588
                     /locus_tag="JNY73_003825"
     CDS             4184..4588
                     /locus_tag="JNY73_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAY8049653.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            4641..4751
                     /locus_tag="JNY73_003826"
     CDS             4641..4751
                     /locus_tag="JNY73_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049654.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
BASE COUNT         1548 a         1025 c          973 g         1524 t
ORIGIN      
        1 ccattcattc gctcgcgctg gcatctacag agatgcatgg tagagagcat aagaaatatc
       61 cacgccgaag ataaaatttt ctccatcttg atctaaccca ttaattattg agcataatgt
      121 aggcagtaca aaataagtta ggcgggatat caggcgtcaa gaatggagag cgagaactct
      181 ccattcttga cacctgatat tgcggacata ataagaaagc ataacgcctg aaatgctcac
      241 tttgcatcag catggtgata cagctgatgt ttattctaaa accttactca agttctaaga
      301 gagcacggat tccctgtcat taataatgat atccgttagt gtagtcttat gcatttcaat
      361 gtactgcaat atttgcagat agagatcaga aagaatcaca ctggtttcat cttttaaacg
      421 caagcgtaaa gcctgatcaa taatatgact atcaacatac ccatgcgtca tgaacacctc
      481 cctctcgaga tttttaaaca aagcacattg aaaaataaat ctctctggga ttgaattatt
      541 catgtcagcc tcgtagaaat attaacccaa taaaattatg ctgtgtatag tctaaagcga
      601 cagacaaaaa taagatgaat gagttttcta taaacttata cttaataatt agaagttaca
      661 tatcatcagc tgtgtatcgc aacacgattt ccagtgtttt tctaatcaca tcagctttga
      721 gaatatcact tgttgtttcc agggagtgta tcagacttaa gataatattt ttattattaa
      781 cattatcgcc tgaaagcatt aaattggtga cagcctgccc taacactgct gactcttctt
      841 ctaacaaatt acccgaactg cgaaagtaac ttgctaacgc tttatcagtt attgcattat
      901 gaattgtagt atcttcaagc atggtaacct ctcatcttac ttatgaaatt ttaatgtatt
      961 ctgtttattt tcttaccatt gaacatactt ttattgtcag ctgtcgctgt tcttcggttt
     1021 ggccctcctg aatcactatt gctaaaatca cgaattatgc tgtcaattaa atcgcgtcgg
     1081 aaagcatctt cctcagccaa tttcattacg agtaattgct taataagagt ctgcaatgaa
     1141 aaatttatct tcatgcgcag tatctggagc accgcctggc cagtaatttc atattttata
     1201 cgttcattat cagatgtctt cataacgact tcatagctaa cctgttaaat aatcaggctg
     1261 ttgcattatt caaaaagcaa cccaattagt gcattataat gtttctgttc ctcttcccct
     1321 gtcgcttgtt ccagtcggca aagaagtttg gcacaatgcg actttcgact cagattacgt
     1381 ccatctttca aaatctcagt cacaatttcc ccgagagttt cctgctgagt aggcaagtta
     1441 gcttttgcaa agtaacgagt gattgctccc gcagaatctg aagtcactga attttgatgc
     1501 atgctacgcc tctgttaaaa atgttaatca atgtgaagtg aggtaacaat agcaaacttg
     1561 tacgaaatat gtgtacagaa tgataaatta attttttgca attagtacta atgtatataa
     1621 aacaatgtgt tatgataaaa catattgcat catttaactg acaatctata caagattttt
     1681 cgccaactcg tgttcaactt tttccatttt ttatcaatgt aaagaaacgg ttatgttttg
     1741 gtattaaaag aaagacacaa gaagatatgt ctgttaccgt cttactctcg cctcacccat
     1801 taccctggat tgttaatgct taccaccctt atttatcgta gccatatacg tgacgacgaa
     1861 cctgtcaaaa aaatcgaaga aatggtttcg atagcaaatc gcaggaacat gcagtctgac
     1921 gtaacaggga tcttactgtt taatggttct cattttttcc agcttctgga aggtccggaa
     1981 gaacaggtta aaatgatata tcgggctata tgccaagatc cacggcacta taatattgtt
     2041 gagctgctgt gcgattacgc gcctgctcgc cgttttggca aagcgggaat ggaattattt
     2101 gatttgcgcc tgcacgagcg agatgacgtt ttacaggccg tattcgacaa aggcacatca
     2161 aaatttcagc taacttatga tgacagagcg ctacaatttt ttcgtacttt tgtccttgca
     2221 accgaacaat caacctattt cgagatccct gccgaagact cctggctttt tatcgctgac
     2281 ggatctgata aagaacttga ttcctgtacc ctttcaccaa ctataaacga ccactttgcc
     2341 tttcatccta ttgtcgatcc cttatcgcgg cggataatcg cttttgaagc cattgtgcaa
     2401 aaaaatgaag atagcccatc agccatagcg gttgggcagc gtaaagacgg ggaaatctac
     2461 acagcggatc tcaaaagtaa ggcgcttgca ttcgcgatga cacacgcact tgagctcggt
     2521 gataaaatga tttcaatcaa tctattacct atgaccctgg ttaacgaacc tgacgcagtc
     2581 tcttttttac ttgatgaaat aaaggccaat gctctggtgc ctgaacaaat catcgttgaa
     2641 tttactgaaa gtgaagttat atctcggttt gatgagtttg cagaagcgat taaatcgctt
     2701 aaggctgccg gtatcagtgt agcaattgat cattttggcg caggttttgc cggtttgtta
     2761 ctcctgtcac gcttccagcc tgacagaatt aaaatcagtc aggaattgat taccaatgtt
     2821 cataaaagcg ggccacggca ggcaattatt caagcgatca taaaatgctg tacatcactt
     2881 gaaattcaag tcagtgctat gggcgtggca acaccagaag agtggatgtg gcttgaatcc
     2941 gcaggaattg agatgtttca gggagatctg tttgctaaag ctaaattgaa tggtatccct
     3001 tccatagcgt ggccagagaa aaaataattt tcagcacatt ctttcacatg atttcagtaa
     3061 atcatcgtga aaatgaatac gttatgttta aatttaatct acattaaaaa taatgtttca
     3121 tccaatttag ctgaaatctt atttatactt tgtacaattt tagcgtatat tgtacaaagt
     3181 attattgggt cgtgtacagg cgacggagat ttgtgaccgc aaggaggaat tgtggcttat
     3241 tacagcattg gtgatgttgc tgaacgttgc gggattaatc ctgtcactct ccgggcctgg
     3301 caacgccact acggtttgtt aaaaccacag cgcagtgaag gcggacaccg actctttgat
     3361 gaagaagaca tacaacgcat cgaagagatc aagcgttgga tcagtaatgg cgtccctgta
     3421 ggcaaagtta aagcattact ggaaaccacc agccaggata cggaagatga ctggagccgc
     3481 ctgcaagaag agatgatgtc aattcttcgt atggctaatc ctgccaaact acgcgcgaga
     3541 attatttcac tgggtcgaga gtacccagta gatcaattga ttaatcatgt ttaccttcct
     3601 gttcgccagc gtctcgtgct tgatcacaac acctcccgca ttatgagcag tatgtttgac
     3661 ggcgcattaa ttgaatacgc agcaacctcg cttttcgaaa cgcgccgtaa gcccggtaaa
     3721 gaagccattc tgatggcgtg gaatgttgaa gagagagcac gattgtggct ggaagcatgg
     3781 cgtttatcat tgtcaggatg gcacatttct gttcttgctg atcccattga atcgccgcgc
     3841 ccggaactgt tcccgacgca aacattgatt gtctggacag gcatggcacc aacgagaagg
     3901 caaaacgaac ttttacaaca ttggggtgag caaggttata aagtcatctt tcatgccccc
     3961 taatttcacc tttatcccgc cccttagaca gaatgttata atctgaaccc acaacagcat
     4021 ttgtaccgga gctggtccgt tgcaaaatgc tcaccgggag aattcaggta atccctctat
     4081 cacactctgt acatatcaca gccaaaggac agaccagatt gaagtggtaa aaccgatcgt
     4141 gcacactacg cagtctcaca actaacaaga ggttattatc ctgatggatc aggtcgttgt
     4201 ttttcaaaag atgtttgagc aagtgagaaa agagcaaaac ttctcctggt tttattcaga
     4261 attaaaacat caccgtattg cacattacat ttattatctg gctacggata acatcagaat
     4321 tattactcac gatgacacgg ttttgttatt aagaggaacc aggaaccagt taaaagttag
     4381 tacgaccaag aatcctgcta aaataaaaga ggccgcattg cttcatattt gcggaaaatc
     4441 tacatttcgg gaatactgtt caacactagc aggcgcgggc gttttccgtt gggttactga
     4501 tgttaatcat aacaaacgca gttactacgc cattgataat acgcttttat atattgaaga
     4561 tgtagaaaat aataaaccat taatctagct aaagttggat gcttaagaaa tgcttcataa
     4621 ttcagtaagg cattagcata atgggaataa aagtacagag actatcccta tggatgataa
     4681 atactgtcct tttattgcta cccataaata atcaccaagc ccatactatc aaattgatat
     4741 ttgaaatgtg atcacttgac tttttatacg ttattttata acggttaata tatttataaa
     4801 aacaacgggt gtgccatacg cccgtttcaa tacttaacgc atatgtgttt tggtttagtc
     4861 atcatccagt tatatgtatt ttagccagga acaggttaaa cctttcctat ataactcaaa
     4921 aattgaaacc ttattctcat gtcatgttta tattcattat tatcgttata taaaaaggca
     4981 accataatgt ttagcaaatt ggcaccaagt agcataaagg ctatgtttta attacaggat
     5041 gttcattcat ttgaatgtat aacattatag
//
LOCUS       DADLZM010000200         5065 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLZM010000200 DADLZM010000000
VERSION     DADLZM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.204"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            138..659
                     /locus_tag="JNY73_003827"
     CDS             138..659
                     /locus_tag="JNY73_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY8049655.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            715..948
                     /locus_tag="JNY73_003828"
     CDS             715..948
                     /locus_tag="JNY73_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY8049656.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            1012..2970
                     /locus_tag="JNY73_003829"
     CDS             1012..2970
                     /locus_tag="JNY73_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY8049657.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     SFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3025..3459
                     /gene="psiB"
                     /locus_tag="JNY73_003830"
     CDS             3025..3459
                     /gene="psiB"
                     /locus_tag="JNY73_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAY8049658.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3456..4175
                     /locus_tag="JNY73_003831"
     CDS             3456..4175
                     /locus_tag="JNY73_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAY8049659.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4397..4609
                     /locus_tag="JNY73_003832"
     CDS             4397..4609
                     /locus_tag="JNY73_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FlmC"
                     /protein_id="HAY8049660.1"
                     /translation="MSSPHQDSLLPRFAQGEEGHETTTKFPCLVCVDRVSHTVDIHLS
                     DTKIAVRDSLQRRTQGGGGFHGLRIR"
BASE COUNT         1150 a         1329 c         1575 g         1011 t
ORIGIN      
        1 tgcggcgaag gttctcaaac aggtgatggt gaataccgga gggaacaaag agcaggtccc
       61 cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaacatca
      121 acagaaggag acacatcatg gcagttcgtg gcattaacaa ggtcattctt gtcggtcgtc
      181 tgggaaaaga tccggaagtc cgttacatcc ccaacggggg cgcagtggca aacctgcagg
      241 tggccacgtc agaaagctgg cgtgacaaac agacggggga gatgcgggag cagacagaat
      301 ggcatcgcgt ggtgctgttc ggcaagctcg cggaagtggc aggtgaatat ctgcgcaagg
      361 gcgcgcaggt ctacatcgag gggcaactcc gaacccgtag ctgggaagat aaaggtatta
      421 cccgttacgt cactgaaatc cttgtgaaaa ccacgggcac catgcagatg ctggggagtg
      481 caccacagca gaacgctcag gcgcaaccgc agcctcagca gaatggtcag ccacagagtg
      541 ctgacacgac gaaaaaaggg agcgcgaaaa caaaaggccg tggacgtaag gccgcgcagc
      601 cggagcctca gcagccgtct gaacccgcgt atgacttcga tgacgacatt ccgttctaac
      661 gaaagacagt gacaaccgcc ccgtccagtg cggggcatca ccggagatat gaggatgagc
      721 gaatatttca gaatatttca ggggctgcct gacggctcct ttacccgcga acaggctgaa
      781 gccgttgccg cacagtaccg gaatgtcttt atcgaggatg atcagggaac gcattttcgc
      841 ctggttgtcc gcaataacag tggcctggtc tggcgtacct ggaattttga ggacggtgcc
      901 gggtactgga tgaaccatgt catccggaat ttcggcattc ttaagtaaga gaggtgccgg
      961 acgggaacgc gtccggcaga catacaacca tcccggtcaa aggagaaata tatgtcagtt
     1021 acagagtcta agacaaaaac ggagcgtaaa tccagccgta aacctgcaaa aacgcaggaa
     1081 acagtcctgt ctgccctgct ggagcagacg gaggaagtga gcgtgtcgct ggcctcgctg
     1141 attaaatcac cgctgaatgt gcgcacggtg ccgtattccg cagagtccgt cagcgaactg
     1201 gcggagtcca ttaagggcgt tggcctgttg cagaatctgg ttgttcatgc cctgcctggt
     1261 gaccgccacg gtgtcgccgc aggtggtcgc cgactggcag cactcaacat gctggcagag
     1321 cgtggcatcc ttccggctga ctggcctgta cgcgtgaagg ttattccgca ggagctggcg
     1381 acggctgcat caatgactga aaacggtcag cgtcgggata tgcaccctgc cgaacagatt
     1441 gccggattcc gcgcaatggc gcaggaaggc aaaacacctg cacaaatcgg tgatttgctg
     1501 ggctattcac cccgccacgt tcagcgaatg ctgaaactgg ctgaccttgc gcctgtcatc
     1561 ctcgatgcgc tggcagaaga ccgcatcacc accgaacact gtcaggcgct ggcgctggag
     1621 aacgacaccg cgcgtcaggt gcaggtgttt gaagccgcct gtcagtcggg atggggcggt
     1681 aaaccggaag tacagaccat tcgtcgtctg gtgaccgaaa gtgaagtggc ggtggcgggg
     1741 aacagtaaat tccgcttcgt gggggctgat gccttctcgc cagacgaact gcgcaccgat
     1801 ttgttcagcg acgacgaagg tggctatgtg gactgcgtgg cgctcgatgc cgccctgctg
     1861 gaaaaactcc aggctgtcgc agagttcctt cgggaagccg aaggctggga atggtgcgcc
     1921 ggacgcatgg agcctgtcgg tgagtgccgt gaggatgccg gaacataccg ctgtctgccg
     1981 gagccggaag cggtgctgac ggaggcggaa gaagaacgtc tgaacgaact gatgacgcgt
     2041 tacgacgcgc tggaaaacca gtgtgaggaa tctgacctgc tggaagcaga aatgaagctg
     2101 atgcgctgca tggcgaaggt cagggcgtgg acgccggaga tgcgtgccgg aggtggagtg
     2161 gtggtgtcct ggcgttatgg caacgtgtgt gtccagcgtg gtgtgcagtt gcgcagtgag
     2221 gatgacgtgg ctgacgacgc tgaccgtacg gaacaggtgc aggagaaagc gtcagtggag
     2281 gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggca
     2341 gcactgatgc agcaaccgga caaatccctg acactgctgg catggacgct ctgcctgaat
     2401 gtgtttggca gcggagcgta cagtaaacca gcacaaatca gcctggaatg taaacattat
     2461 tcgctgacca gcgatgcgcc atcggggaag gaaggtgccg cattcatggc gctgatggca
     2521 gaaaaagccc gtcttgctgc cctgttaccg gagggatggt cacgggacat gacgacattc
     2581 ctgtcactca gtcaggaggt gctgttatcc ctgctcagtt tctgcaccgc atgcagcctt
     2641 aacggtgtcc agacccgtga gtgtggtcac acgtcacgca gtccgcttga cacgctggaa
     2701 agcgccatca gctttcacat gcgcgactgg tggcagccga caaaagcaaa cttcttcgga
     2761 cacctgaaaa agccgcagat tatcgcagcc ctgaatgagg cagggctgtc cggtgccgca
     2821 cgggacgcgg agaagatgaa gaaaggtgat gcggctgaac atgcagagca ccatatgaaa
     2881 gacaaccgct gggtgcctgg ctggatgtgt gcaccacatc cacagacaga taccactgaa
     2941 cgcaccgata acctggctga tgccgcctga tgaacaacca caccgccccg ccggagacgg
     3001 ggcggcagca agggagatac cgtgatgaaa actgaactga ccctgaatgt attacagacc
     3061 atgagcgcac aggaatatga agatatccgg gctgccggaa gtgatgaacg tcgtgagctg
     3121 acacacgccg tgatgcggga actggatgcg ccggacaact ggacgatgaa cggcgagtac
     3181 ggcagcgagt ttggcggatt tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt
     3241 tttcacctgg cgttatgttc accgggcgat gtctctcagg tctgggtgct ggttctggtg
     3301 aatgccggtg gtgagccgtt tgcggtggtt caggtacagc ggcggtttgc accggaagcc
     3361 gtcagccatt cactggcact ggcggcgtca ctggacgcgc aggggtacag cgtcagtgac
     3421 atcatccata tcctgatggc ggaaggaggt caggcatgag cgcacgttca caggcactgg
     3481 ttcccctcag cgcagagcaa caggccgcat ggcgggcggt ggcggagaca gaaaaacgcc
     3541 gccaccaggg aaacacgctg gcggaatatc cgtatgcggg ggcgtttttc cgctgtctga
     3601 acggaagccg caggatatcg ctgtctgacc tgcgattttt catgccgtcc ctgaccgcag
     3661 aagaactgca cggcagccgt cttcagtggc tgtatgccat cgatgtgctg attgaaacac
     3721 agggcgaggt ctgcctcctt cctcttccag gcgatgcagc agaacgcttg ttcccgtcgg
     3781 tgcggtttcg tgtcagggaa cgcagtcgcc ataaatccgc gctggtcatg cagaagtaca
     3841 gccgccagca ggcgagggag gcagagcaga aagcccgtgc gtaccaggcg ctggtcgctc
     3901 aggctgaaat cgaactggca ttccattcgc cggaaacagt cggaagctgg tatgcacgct
     3961 ggagtgaccg ggtggcagag catgacctgg aaaccctgtt ctggcagtgg ggtgagcgtt
     4021 ttccgtcact ggccggaatg gaacgctggc agtggcagga tatgccgttc tggcaggtta
     4081 ttgcggaagc cagtcttgca gcaagagagg ccggtcacgc tgtccgtgag atggagcggt
     4141 ggatggtgcc gaacaaactc cgggaggcgg cgtgatgcgg caacaatcac acagattacc
     4201 cgtaaacagc ctgaatgagc gggttatttt caggaaaagt gagtgtggtc agcgtgcagg
     4261 gatatgggct atgatgtgcc cggcgcttga ggctttctgc ctcatgacgt gaaggtggtt
     4321 tgttaccgtg ttgtgtggca gaaagaagat agccccgtag taagttaatt ttcattaacc
     4381 accacgaggc atccctatgt ctagtccaca tcaggatagc ctcttaccgc gctttgcgca
     4441 aggagaagaa ggccatgaaa ctaccacgaa gttcccttgt ctggtgtgtg ttgatcgtgt
     4501 gtctcacact gttgatattc acttatctga cacgaaaatc gctgtgcgag attcgttaca
     4561 gagacggaca cagggaggtg gcggctttca tggcttacga atccggtaag tagcaacctg
     4621 gaggcgggcg caggcccgcc ttttcagggc tgatgctggt ctgactgcac tgaagcgcct
     4681 ttataaaggg gctgctggtt cgccggtggc ccctttctcc ttgctggcat tgtacgggca
     4741 tgaacaacca gactgccagg aaggccgttt ccctccggga acggggatga acgtgcgcct
     4801 gcggcgcccg ttcttttccc ccgccttctc tgtttatgac ttaccagaaa tcacatcacc
     4861 ttccctttga ttattctctt tttcctcctg ctctgactct gattactgct ctgactctga
     4921 ttactgctgc cctgattatt gtctgttttg ttgttctcct gcttatgcct tttccgggcg
     4981 tccccctggc ggggcgggct gtcgtgaacg gagccagcga agctgtctcc gccctggcgg
     5041 gcttccatcc ctgacgcaaa aaccc
//
LOCUS       DADLZM010000201         5026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLZM010000201 DADLZM010000000
VERSION     DADLZM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.205"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            297..1241
                     /gene="leuO"
                     /locus_tag="JNY73_003833"
     CDS             297..1241
                     /gene="leuO"
                     /locus_tag="JNY73_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY8049661.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            1559..3283
                     /gene="ilvI"
                     /locus_tag="JNY73_003834"
     CDS             1559..3283
                     /gene="ilvI"
                     /locus_tag="JNY73_003834"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY8049662.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3286..3777
                     /gene="ilvN"
                     /locus_tag="JNY73_003835"
     CDS             3286..3777
                     /gene="ilvN"
                     /locus_tag="JNY73_003835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY8049663.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3789..3875
                     /locus_tag="JNY73_003836"
     CDS             3789..3875
                     /locus_tag="JNY73_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY8049664.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3957..4961
                     /gene="cra"
                     /locus_tag="JNY73_003837"
     CDS             3957..4961
                     /gene="cra"
                     /locus_tag="JNY73_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY8049665.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1229 a         1138 c         1317 g         1342 t
ORIGIN      
        1 tataaaataa ttattaaata agcacattta atccattttg tagatgattg agtattcgcg
       61 gtagttatga ttagattgtt ttcgcaacaa aaacattatg gattattatg ctgtggtaaa
      121 tgactcattc cacggcaatg gattctgttt ttatcagaac ccgtatcttt atgttttccg
      181 aattttactc attttgcttt ttcttatttt atatgcatga taaatcatat tcttcaggat
      241 tatttctctg cattccaata agggaaaggg agttaagtgt gacagtggag ttaagtatgc
      301 cagaggtaca aacagatcat tcagagacgg cggagttaag caagccacag ctacgcatgg
      361 tcgatctcaa cttattaacc gttttcgatg ccgtgatgca ggagcaaaac attacccgtg
      421 ccgctcatgt tctgggtatg tcgcaacctg cggtcagtaa cgctgttgca cgcctgaagg
      481 tgatgtttaa tgacgagctt tttgttcgtt atggccgtgg tattcaaccg actgctcgcg
      541 catttcaact ttttggttca gttcgccagg cattgcaact agtacaaaat gaattgcctg
      601 gttcaggttt tgaacccgcg agcagtgaac gtgtatttca tctttgtgtt tgcagcccgt
      661 tagacagcat tctgacctcg cagatttata atcacattga gcagattgcg ccaaatatac
      721 atgttatgtt caagtcttca ttaaatcaga acactgaaca tcagctgcgt tatcaggaaa
      781 cggagtttgt gattagttat gaagacttcc atcgtcctga atttaccagc gtgccattat
      841 ttaaagatga aatggtgctg gtagccagca aaaatcatcc aacaattaaa ggcccgttac
      901 tgaaacatga tgtttataac gaacaacatg cggcggtttc gctcgatcgt ttcgcgtcat
      961 ttagtcaacc ttggtatgac acggtagata agcaagccag tatcgcgtat cagggcatgg
     1021 caatgatgag cgtacttagc gtggtgtcgc aaacgcattt ggtcgctatt gcgccgcgtt
     1081 ggctggctga agagttcgct gaatccttag aattacaggt attaccgctg ccgttaaaac
     1141 aaaacagcag aacctgttat ctctcttggc atgaagctgc cgggcgcgat aaaggccatc
     1201 agtggatgga agaacaatta gtctcaattt gcaaacgcta actgaatgca gaataggcca
     1261 gacatgaatg tctggtttat tctgcatttt ttattgaatg tagaatttta ttctgaatgt
     1321 gtggactctc tattttagga ttaattaaaa aaatagagaa attgctgtaa gttgtgggat
     1381 tcagccgatt tattatcaat ttaatccttc gtaatggagg attttatcgt ttcttttcac
     1441 ctttcctcct gtttattctt attaccctgc atttatgtct ctggctgcca attgcttaag
     1501 caagatcgga cggttaatgt gttttacaca ttttttccgt caaacagtga ggcaggccat
     1561 ggagatgttg tctggagccg agatggtcgt ccgatcgctt atcgatcagg gcgttaaaca
     1621 agtattcggt tatcccggag gcgcagtcct tgatatttac gatgcattgc ataccgtggg
     1681 tggtattgat catgtattag ttcgtcatga gcaggcggcg gtgcatatgg ccgatggcct
     1741 ggcgcgtgcg accggggaag tcggcgtcgt gctggtaacg tcgggtccag gggcgaccaa
     1801 tgcgattacc ggcatcgcca ccgcttatat ggattccatt ccattagttg tcctttccgg
     1861 gcaggtagcg acctcgttga taggttacga tgcttttcag gagtgcgaca tggtggggat
     1921 ttcgcgaccg gtggttaaac acagttttct ggttaagcaa acggaagaca ttccgcaggt
     1981 gctgaaaaag gctttctggc tggcggcaag tggtcgccca ggaccagtgg tcgttgattt
     2041 accgaaagat attcttaatc cggcgaacaa attaccctat gtctgccctg agtcggtcag
     2101 tatgcgttct tacaatccca ctactaccgg acataaaggg caaattaagc gtgctctgca
     2161 aacgctggta gcggcaaaaa aaccggttgt ctacgtaggc ggtggggcaa tcatggcggg
     2221 ctgccatcag cagttgaaag aaacggtgga ggcgttgaat ctgcccgttg tttcctcatt
     2281 gatggggctg ggggcgtttc cggcaacgca tcgtcaggca ctgggcatgc tgggaatgca
     2341 cggtacctac gaagccaata tgacgatgca taacgcggat gtgattttcg ccgtcggggt
     2401 acgatttgat gaccgaacga cgaacaatct ggcaaagtac tgcccaaatg ccactgttct
     2461 gcatatcgat attgatccta cttccatttc taaaaccgtg actgcggata tcccgattgt
     2521 gggggatgct cgccaggtcc tcgaacaaat gcttgaactc ctgtcgcaag aatccgccca
     2581 tcaaccgctg gatgagatcc gcgactggtg gcagcagatt gaacagtggc gcgctcttca
     2641 gtgcctgaaa tatgacactc acagtgaaaa gattaaaccg caggcggtga tcgagactct
     2701 ttggcggttg actaatgggg acgcttacgt gacgtccgat gtcgggcagc accagatgtt
     2761 tgctgcgctt tattatccat tcgacaaacc gcgtcgctgg atcaattccg gcggtctcgg
     2821 cacgatgggt tttggtttac ctgcggcact gggcgtcaaa atggcgttgc cagaagaaac
     2881 cgtggtttgc gtcactggcg acggcagtat tcagatgaac atccaggaac tgtctaccgc
     2941 gttgcaatac gagttgcccg tactggtggt gaatctcaat aaccgctatc tggggatggt
     3001 gaaacagtgg caggacatga tctattccgg ccgtcattca caatcttata tgcaatcact
     3061 acccgatttc gtccgtctgg cggaagccta tggtcacgtc gggatccaga tctctcatcc
     3121 gcatgagctg gaaagcaaac ttagcgaggc gctggaacag gtgcgcaata atcgcctggt
     3181 gtttgttgat gttaccgtcg atggcagtga gcacgtctac ccgatgcaga ttcgcggggg
     3241 cggaatggat gaaatgtggt taagcaaaac ggagagaacc tgattatgcg ccggatatta
     3301 tcagtcttac tcgaaaatga atcaggcgcg ttatcccgcg tgattggcct tttttcccag
     3361 cgaggctaca acattgaaag cctgaccgtt gcgccaaccg acgatccgac attatcgcgt
     3421 atgaccatcc agaccgtggg cgatgaaaaa gtacttgagc agatcgaaaa gcaattacac
     3481 aagctggtcg atgtcttgcg cgtgagtgag ttggggcagg gcgcgcatgt tgagcgggaa
     3541 atcatgctgg tgaaaattca ggccagcggt tacgggcgtg acgaagtgaa acgtaatacg
     3601 gaaatattcc gtgggcaaat tatcgatgtc accccctcgc tttataccgt tcaattagca
     3661 ggcaccagcg ataagcttga tgcattttta gcatcgattc gcgatgtggc gaaaattgtg
     3721 gaagttgctc gctctggtgt ggtcggactt tcgcgcggcg ataaaataat gcgttgagaa
     3781 tgatctcagt gcgcaatttg cagcccaaca tgtcacgttg ggcttttttt gcgaaatcag
     3841 tgggaacctg gaataaaagc agttgccgca gttaattttc tgcgcttaga tgttaatgaa
     3901 tttaacccat accagtacaa tggctatggt ttttacattt tacgcaaggg gcaattgtga
     3961 aactggatga aatcgctcgg ctggcgggag tgtcgcggac cactgcaagc tatgttatta
     4021 acggcaaagc gaagcaatac cgtgtgagcg acaaaaccgt tgaaaaagtc atggctgtgg
     4081 tgcgtgagca caattaccac ccgaacgccg tggcagctgg gcttcgtgct ggacgcacac
     4141 gttctattgg tcttgtgatc cccgatctgg agaacaccag ctatacccgc atcgctaact
     4201 atcttgaacg ccaggcgcgg caacggggtt atcaactgct gattgcctgc tcagaagatc
     4261 agccagacaa cgaaatgcgc tgcattgagc accttttaca gcgtcaggtt gatgccatta
     4321 ttgtttcgac gtcgttgcca cctgagcatc ctttttatca acgctgggct aacgacccgt
     4381 tcccgattgt cgcgctggac cgcgccctcg atcgtgaaca tttcaccagc gtggttggtg
     4441 ccgatcagga tgatgccgaa atgctggcgg aagagttacg taagtttccc gccgagacgg
     4501 tgctttatct tggcgcgctg ccggagcttt ctgtcagctt cctgcgtgaa caaggtttcc
     4561 gtactgcttg gaaagatgat ccgcgcgaag tgcatttcct gtatgccaac agctatgagc
     4621 gggaggcggc tgcccagtta ttcgaaaaat ggctggaaac gcatccgatg ccgcaggcgc
     4681 tgttcacaac gtcgtttgcg ttgttgcaag gagtgatgga tgtcacgttg cgtcgcgacg
     4741 gcaaactgcc ttctgacctg gcaattgcca cctttggcga taacgaactg ctcgacttct
     4801 tacagtgtcc ggtgctggca gtggctcaac gtcaccgcga tgtcgcagag cgtgtgctgg
     4861 agattgtcct ggcaagcctg gacgaaccgc gtaagccaaa acctggttta acgcgcatta
     4921 aacgtaatct ctatcgccgc ggcgtgctca gccgtagcta agccgcgaac aaaaatacgc
     4981 gccaggtgaa tttccctctg gcgcgtagag tacgggactg gacatc
//
LOCUS       DADLZM010000202         4992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLZM010000202 DADLZM010000000
VERSION     DADLZM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.206"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="JNY73_003838"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="JNY73_003838"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY8049666.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNY73_003839"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNY73_003839"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY8049667.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="JNY73_003840"
     CDS             2152..2610
                     /locus_tag="JNY73_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY8049668.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="JNY73_003841"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="JNY73_003841"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY8049669.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="JNY73_003842"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="JNY73_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY8049670.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="JNY73_003843"
     CDS             3816..4493
                     /locus_tag="JNY73_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY8049671.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="JNY73_003844"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="JNY73_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY8049672.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1213 a         1186 c         1315 g         1278 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac cctaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga atgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatc agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctgggga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg atttcaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tc
//
LOCUS       DADLZM010000203         4951 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLZM010000203 DADLZM010000000
VERSION     DADLZM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.207"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..611
                     /locus_tag="JNY73_003845"
                     /pseudo
     CDS             1..611
                     /locus_tag="JNY73_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
     gene            632..1292
                     /gene="csiR"
                     /locus_tag="JNY73_003846"
                     /pseudo
     CDS             632..1292
                     /gene="csiR"
                     /locus_tag="JNY73_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="JNY73_003847"
     CDS             complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="JNY73_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY8049673.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1826..1984)
                     /locus_tag="JNY73_003848"
     CDS             complement(1826..1984)
                     /locus_tag="JNY73_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY8049674.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2167..2466
                     /locus_tag="JNY73_003849"
     CDS             2167..2466
                     /locus_tag="JNY73_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8049675.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2476..3000
                     /gene="ygaP"
                     /locus_tag="JNY73_003850"
     CDS             2476..3000
                     /gene="ygaP"
                     /locus_tag="JNY73_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY8049676.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="JNY73_003851"
     CDS             complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="JNY73_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY8049677.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4119..4556
                     /gene="alaE"
                     /locus_tag="JNY73_003852"
     CDS             4119..4556
                     /gene="alaE"
                     /locus_tag="JNY73_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY8049678.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4593..4943)
                     /locus_tag="JNY73_003853"
     CDS             complement(4593..4943)
                     /locus_tag="JNY73_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY8049679.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
BASE COUNT         1261 a         1167 c         1165 g         1358 t
ORIGIN      
        1 tgccggggct gaaagccgtt ggatcttatc gctcggttct ggaattgctc aatattcccc
       61 atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagttgc ctgaactcgg
      121 ccctgtatac cgcctcaagg atgctctact ccttaagccg tcgcggtgat gctcccgcgg
      181 taatgggtaa aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc tccaccggcg
      241 cagcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt aaatttctga
      301 tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc
      361 ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg tggctttatc
      421 cctggctcac ctagctggtc atcggcttta ttacctttgt gttggtagtg atgctattcc
      481 gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg attatctgta
      541 ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata
      601 atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg atggctatcg
      661 ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag
      721 tttactgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagtac tttctcaact
      781 ggtggtggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc
      841 agagcaggag ctgctctgac atagacgccc gcgccaatat ggaagcgatg ctggtcagtc
      901 tggcgattgc ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc
      961 tcaataagct ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc
     1021 atcaggcgtt tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc
     1081 gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc
     1141 tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac
     1201 tggcgcgaga tacagcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc
     1261 ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc
     1321 cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg
     1381 tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta
     1441 acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg
     1501 gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg
     1561 ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca
     1621 ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc
     1681 tgacctgtaa ccgcatccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc
     1741 atagctacct cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt
     1801 taacgccgcg ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca
     1861 aaccggggat atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc
     1921 ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac
     1981 ccatatgtac tccctataag aaaattactc attgtttaaa aagagacttt atctcttaag
     2041 cgtagtaatt ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg
     2101 ctgaggctgg tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa
     2161 ataaatatga ctgaactcgc gcaattacag gccagtaccg aacaggcagc ggccttattg
     2221 aaagcaatga gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc
     2281 accagcgcgg gagagctgac gcgcattacc ggactgagcg cctctgcgac atcacagcat
     2341 ctcgctcgta tgcgggacga agggcttatc aacagccaac gggatgccca acgcattcta
     2401 tattccatta aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt
     2461 ccgtaaggag tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc
     2521 gcacgcggcg caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt
     2581 cctgaagcag atctggctcc attatccgtg ctggaacagt caggtcttcc acctaaatta
     2641 cgtcgcgagc aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat
     2701 aaattagcag cgattgccgc ccccgcagaa atatttttac tcgaagatgg gattgatggc
     2761 tggaaaagag ctggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc
     2821 caggtgcaga tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta
     2881 aatagcggtt tcttcttatt aagtggcttt gttggtgccg ggttactgtt tgcaggaatc
     2941 agcggttttt gtggaatggc aaggttgtta gataagatac cgtggaacca acgagcttga
     3001 gaagcgacgc cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat
     3061 cgtcgagaga tttaccttct gccagcgcct gagcaattgg cttcggtgta cggccctgac
     3121 cggtccaggt tttagtttca ccgttaacat cagtgaattt atatttcgcc ggacgcggct
     3181 ggcgtttttt accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt
     3241 taattccgtc agctttcatc agctccagcc aggtgctaat tttttcctga cgctcagcca
     3301 gttcacgctg ctgctgttct tcttcttcac gcctttcttt agtgacaacc ctgaattttt
     3361 cgagcatttc ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa
     3421 tgttatttaa actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca
     3481 aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc
     3541 tatttacagt cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt
     3601 tcaagattat atattagcca ttttgatgag taatttaggt tattggtgaa cagagtggcg
     3661 ctctctgctc gctaactccg taagcctgac aaaaccgaag tgaataaaaa tgcaatataa
     3721 cagattaaaa gaaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca
     3781 tcaaaaatgc tgtaaatgac ccggtcacga caaacaccga attctctacg cttatagcgt
     3841 tacctccccc ccaaactttc attagtgaaa ttaaaggcaa ggccatatcc aattgattta
     3901 attgaaaata aatcatatcg acatgtaaaa atccacaatg tacaaaaaac gagctgttac
     3961 ggaatatttt atctacaaaa actgactaaa taaaaatttt tcactaattg attagtaata
     4021 gccagcgata tacgctatgc gaaaatacag atggcaatga gatccactgc tttcatctcc
     4081 attaacatcc cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct
     4141 tgcgtcatgc agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt
     4201 gtattgaagt tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag
     4261 cgattccggt gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc
     4321 gcgcggcacg caaaggctgg ataaaaaatc tggctgatat cctggcttat gtgacgttcc
     4381 agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg
     4441 cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc
     4501 tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgactct
     4561 tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc
     4621 aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcgtgt
     4681 cgctgtcgct cgcctgctaa atagagcgga aattgctggt aatgatcgca agttttaata
     4741 tcggaagcgg gttcagccag tgttgaactg cgttctttta atgttggatg aattaccaga
     4801 gcggtacgtc ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa
     4861 ccatacgctt tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc
     4921 acctcgtctg gtcgtaaata catatttgat c
//
LOCUS       DADLZM010000204         4825 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLZM010000204 DADLZM010000000
VERSION     DADLZM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.208"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            448..1203
                     /locus_tag="JNY73_003854"
     CDS             448..1203
                     /locus_tag="JNY73_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY8049680.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            1282..1355
                     /locus_tag="JNY73_003855"
     tRNA            1282..1355
                     /locus_tag="JNY73_003855"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1314..1316,aa:Gly,seq:ccc)
     gene            1490..3097
                     /locus_tag="JNY73_003856"
     CDS             1490..3097
                     /locus_tag="JNY73_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8049681.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPDPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVSKATRADMLRVREVLQLLPKNCKQRFKAMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTVLAAITGARLNEVAQLQVKDVRTTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTMGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVNG"
     gene            3090..3743
                     /locus_tag="JNY73_003857"
     CDS             3090..3743
                     /locus_tag="JNY73_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAY8049682.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            4684..>4825
                     /locus_tag="JNY73_003858"
     CDS             4684..>4825
                     /locus_tag="JNY73_003858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049683.1"
                     /translation="MSTQPLMKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSA
                     KEP"
BASE COUNT         1278 a         1149 c         1211 g         1187 t
ORIGIN      
        1 ttcacccgta gcggttgctt cccgcgcttc catagaaatc ccctcgattt gaacatttgg
       61 ttcttatggc aaagatttag tctaagcagg caagatttac gccagaaacg acgataagcg
      121 ttaggggagg aaggaagtgt gatgacgtta aaaaaacaca gatatgatac gaaacaggac
      181 tctgaatagg gacagtgata cgtatcaata tgagtggcga taaaatcaat gtgataaatc
      241 aggattatta cgcatcagga tgggaaatag ggtaaaaagt caggaacttc gatacctcta
      301 cagcgaatat tttgctgatg atacttccct ttgccaaatg aaattaacct ctttctctat
      361 taccacgttt ttccagaagc aagagattgg gtatcaagag gctggctgct atgatatggc
      421 gtcttgtttt ttaagcgagg aaagattttg agtgcgggac gcctgaataa aaaatctctg
      481 ggtatcgtga tgtttttatc ggttggactg ctattggcgg gctgttcggg tagcaaatca
      541 tccgatacag gaacgtattc cggctccgtt tacaccgtga aacgggggga tacgctatat
      601 cgtatttcgc gcaccacggg aaccagcgta aaagagctgg cgcgactgaa cggcatttcc
      661 cccccttaca ccattgaagt tggtcagaaa ctaaaactgg gtggggcgaa aagtagcagt
      721 agtacacgta aatcaaccgc caaatcaacg accaaaaccg catcggttac accgtcatca
      781 gcggtaccga aatcttcctg gccgccagta gggcaacgtt gttggttatg gccaacgaca
      841 gggaaagtta tcatgccgta ttcgacagca gatggcggca ataaagggat tgatatctca
      901 gctccacggg gtacacctat ttacgccgcg ggtgcaggaa aggtggtgta tgtgggcaac
      961 cagctgcgtg gctacggtaa tctcatcatg attaaacaca gtgaagatta cattacggct
     1021 tacgcccata atgacacgat gctggtaaat aatgggcaaa gcgtgaaggc tgggcaaaaa
     1081 atcgccacta tggggagcac ggatgcggca tctgttcgcc tgcatttcca gattcgttac
     1141 cgtgcaacgg caattgatcc gctacgttac ttgccgccgc agggcagcaa gccaaaatgc
     1201 tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc taaggagagc gtaaggttta
     1261 taatgcctta cgcatctcga agcgggcgta gttcaatggt agaacgagag cttcccaagc
     1321 tctatacgag ggttcgattc ccttcgcccg ctccatgaca tttcttaaag ttcaataact
     1381 tactgaatcc taagagatat ggcttaacgc cttccgatag ttttactcac tgtaaccaac
     1441 actgtgacta actttgttga agtagtgagc agacctaagg tctcagtaca tgctcaactc
     1501 aagaacctac ctttatcagc gtaatggtgt cttttacatc cgtcttagga tgaagaccac
     1561 tggtcgcctt accgcttcgc ttccttctca caaccgttat aaactagcgt cagtatcttt
     1621 acggaccaag gatagacgta ccgctatggc tcactcccgg cacatcaagt cagcacttaa
     1681 agcaatccat gcagacaacc ctaacgcctc ttatgaggag ctacgggagc acctgaagac
     1741 cattgtcgag tgggaactca gtgtcagccg cgatgacctg aacgaccctg agtcctatca
     1801 gctttacgtt gaccagtacg acgatattaa gtccaacctt cgggaagctg tagcgactga
     1861 gcacctgact gtagaccagc atcgctatat caatgacgtt atcggtgtgc ttaaagcctg
     1921 tcaggataga ctcaaaggtg atagctctgg tctgttgtct taccttgagc ctgagactgg
     1981 cagtctaaga ccgtccgtct ccctatctgt attggcagaa cctgaagtcc ctgaccctaa
     2041 agctctgacc ctcgcgtctc tcattgagca gtacgagcag gagaacgccc agaactggaa
     2101 gccagcgacc ctgagcgaga accgagcgtc gcactccacg ctaatcgaga tattcgacta
     2161 tctggatatt caggacgtca gcaaggcgac ccgtgcagat atgcttaggg tccgtgaagt
     2221 cctccaactg ttaccgaaga actgcaaaca acgctttaag gctatgccgc tgtctgacct
     2281 gctgaaccgg gagtctaaga ctgactgtct ggatgtcgtt accatcaaca ataaatacct
     2341 tatcaagatg gctgctgtgt ttaagtgggc ggtacgcaac gacctgatag ctaagaacct
     2401 gacagaaggt cttgagctga aagtcccgca gcgtaaagcc tccgacgctc gtgatgcgtt
     2461 cagtcctgag caggtagggc aactactggt cgcagctaaa gcgtactcgc agaagacctc
     2521 agggaagcca taccattatt acgtcaccgt tctggctgca atcactggag cgagacttaa
     2581 cgaggtggcc caacttcagg tcaaagacgt caggaccact gaggccggga ccgtgtatat
     2641 ccatatcaac gaagacgata gcagcttgcc gggtaagagc atcaagaacg cccacagtga
     2701 ccgctgtgtc ccgctggtcg atggcgctta tggttttgta ttggctgact ttatgtcgct
     2761 tgtggaagac cgtagaaaga ctatgggtga taatgctatg gtattcgatg gcctcaagct
     2821 gatgaagaac ggctatggtg agcaggtgag caaatggttt aaccgaacgt tgctacctaa
     2881 agtccttgct gaccgtagcg gcttagcgtt tcactcgttt agacatacag ttgcgaccca
     2941 gctaaaacaa catggggtcg aattggcgta tgctcaggcg attatgggtc atagctctgg
     3001 gtctataact tacgaccgtt acgcgaaaga ggtcgaggta gatagattgg ttaatgtgat
     3061 ggcgggtgtt tataaggaga ctggagtaaa tggctgagta ttatccggca gagtttggtg
     3121 gcactgcttt taactgcccg aattgtggcg cttattctca acagcgctgg gtccgcttaa
     3181 aatatggcgc taacgggatt tatgagacac ctttcgcctt ttctaaatgt gaacgatgca
     3241 atgagaaagc ttattggtat gacgaaacca tgattatacc agcgtcagca aacgttgaga
     3301 tgcctaaccc agacatgcca gacgactgta agtccgacta catggaggcc agaagcattg
     3361 ttaacctttc tcctaaaggc gctgccgcac tcctgcgctt aagtcttcag aagctgatgg
     3421 tccatctcgg tgagccgggg aagcatatcg acacggacat taagtcgctt gtagccaaag
     3481 gattgtctcc attggtccag cgagctgctg atatttgtcg tatcgttggc aatcaggcgg
     3541 tccatccggg tgaaattaat attgatgacg accctcagtt agcgcatggt ctttttaagc
     3601 tgctgaatat tattgtcact gagcagatta ccagaccgaa agaagtcgaa gccatgttta
     3661 acagcatgcc ggaaagggcg ctcaagggca ttgaggaccg ggacaggaag gcgagggaac
     3721 agcagcaggc agctagcgag taaagcttta ggtcagattg aatataccct cactataggg
     3781 aagggggctt aactatcact ataggggaga gaccctaagt ccccacctta atagcagtca
     3841 cgccccatac gctcattcat tttagtctca ggcttccggt agcggaccga ttggaaacca
     3901 tccgcagacg cctagtgtta aatcaccttg gcctgagacc attctccttt atcgtagcaa
     3961 tctctctcct tgacaggcgg agaacatcag accgcagcgg aagtatccgt ctatgcgacc
     4021 atcggtgtgc gagattgtga agaaccaaga catcattgct gaggtctgcc atccattgtg
     4081 gacaggcgga aactcaaaga ggttagacca gtagtggtgt ctgaggtttg ggctttgagt
     4141 cctgaccagc aggctggagg agaccatgag tttatcttgt tatggattgt gttatcacaa
     4201 ttccaataag aagaacttta agtccagcct ccagtcctgt cagcccgaag taaactatgg
     4261 tcgctatgat gtggtctcca tacttcgggt ctaactggag gtcagattat ggccttatca
     4321 ggtagcaaat cttacaccct cactgtagag accatacatt ccgcctctgg tcttgatgcg
     4381 tcaagttatg cggaagctct cccgacatta agtctttctc cgaagacatc ccaaggcaag
     4441 acagcctctg tcagacctcc agcccctaga agttcttctc ctcttgaagt ctatcagctt
     4501 ctagcatctg gtcgtcctga cacgctgtcg aaccttcata tctatggtca tcgcagacca
     4561 catcacaatt acctcaagtc tttctgtatg tctcctgttg cctgtgagtc tccctatcgt
     4621 tgggggcttg agtaggctct cagggtgtga cttcaggagg cataacaaaa ggagtacaac
     4681 gatatgtcaa ctcaaccctt aatgaagcat gaagtagttc ttcctgtcat cgttgacggc
     4741 tatcactggg cctgtaatca ggaccgtatg tggaacctga acgagctaca taaagagctt
     4801 gagcttccat ctgctaagga gccgg
//
LOCUS       DADLZM010000205         4779 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLZM010000205 DADLZM010000000
VERSION     DADLZM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.209"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..180)
                     /locus_tag="JNY73_003859"
     CDS             complement(<1..180)
                     /locus_tag="JNY73_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY8049684.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
     gene            complement(244..2073)
                     /gene="recQ"
                     /locus_tag="JNY73_003860"
     CDS             complement(244..2073)
                     /gene="recQ"
                     /locus_tag="JNY73_003860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY8049685.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(2206..3075)
                     /gene="pldA"
                     /locus_tag="JNY73_003861"
     CDS             complement(2206..3075)
                     /gene="pldA"
                     /locus_tag="JNY73_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY8049686.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3240..3707
                     /locus_tag="JNY73_003862"
     CDS             3240..3707
                     /locus_tag="JNY73_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY8049687.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3759..4649
                     /gene="rarD"
                     /locus_tag="JNY73_003863"
     CDS             3759..4649
                     /gene="rarD"
                     /locus_tag="JNY73_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY8049688.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
BASE COUNT         1131 a         1249 c         1214 g         1185 t
ORIGIN      
        1 caggccaagc aacgcaatcc cagcccaaac cattacgccg caggtaatgc ccagcacgcc
       61 catcatcgct tctttacggg aacgactgac agcggtctga gagacaaaaa agaaatcggg
      121 accggggctc ataagcgcca caatgtgcac catggcgacg gtgagaaata acatcaacat
      181 acatttgact cgcgggggaa acgtttacgg agtcttcata ctggcacttt tttatgctgc
      241 tgactactct tcgtcgtcgc catcgacatg cgcacgaatc agcgccataa acggtttacc
      301 aaagcgttcc agcttgcgca tcccaacgcc gttaacgctg agcatttcgc tggcggtgat
      361 cggcatctgt tcagccatct caatcaaggt tgcgtcgtta aacaccacgt acggcgggac
      421 attactttca tcggcaatcg atttacgcag tttgcgtaat ttggcgaaca gtttgcgatc
      481 atagttgccg ccgaacgatt tctgcatcgc tttcggtttg agcgccacga tacgcggcac
      541 ggcaagttgc aaagaggatt cgccgcgcag caccgggcgc gcggcctctg tcagttgtag
      601 ggcagaatgc tgggcaatat tttgcgtcac caggccgaga tgaatcagct ggcggatcac
      661 gctcacccaa tgttcatggc ttttatcacg gcccatacca tagactttca gtttgtcatg
      721 accatagtcg cggatacgct ggttattagc accacgaatc acttccacca cataacccat
      781 cccaaaccgc tgattcacac gaccaatggt ggaaagggca atctgagcat cggttgaacc
      841 gtcgtactgt ttcggcggat cgaggcagat atcgcagttc ccgcacggct cctgacgccc
      901 ttcgccaaaa tagttcagca ataccagacg acggcaagtt tgcgcttcgg caaacgcgcc
      961 catcgcattg agtttgtggc gctcgatatc ctgcaactgc ccctgcggct tctcttccag
     1021 acaacggcgc agccacgcca tatcagccgg atcgtaaaac agcatcgctt ccgcaggcag
     1081 gccatcacgc ccggcgcgac cggtttcctg ataataggat tcgatattgc gcggaatatc
     1141 aaagtggacc acgaagcgaa cgtttggttt attgatgccc atgccgaacg ccaccgtcgc
     1201 caccacaatt tgcagatcat cgcgctggaa tttctcctgc acgtcggcgc ggacattatt
     1261 ttccagcccg gcatgatagg ccgccgcgct aatacccttg ctttgcaggc gcgcagcggt
     1321 gtcttctact ttcgcccggc tgttgcagta gataatgcca gacttaccgc gctgttcctg
     1381 cacgtagcgc atcaactgat cgagcggttt gaatttctcc atcagcatgt agcgaatatt
     1441 cggacggtca aaactgctga tttgaatcag cggatcgttc agccccagca ggcgcacgat
     1501 atcctggcgc gtggtgtcgt cggctgtggc ggtcagcgcc ataaacggca gcgtcgggaa
     1561 ccgctggcgc aactgaccga gcgcggcata ttccgggcgg aaatcgtggc cccattggga
     1621 gatacagtgc gcttcatcga cggctaataa caccggattc cagtgcgcca gatgctcaag
     1681 aaagttatcc agcatcaggc gttccggggc gatatacagt aagcgaattt gcccggtgcg
     1741 gcagcctgtc atcacttcaa gttgctgttc gcgggtttgc gtcgagttaa ggcacgccgc
     1801 cgccacgccg ttggcctgta gttgatccac ctgatctttc atcaacgaaa tcagcggtga
     1861 aacaaccacg gtaaggccgt ttagcaataa ggcaggaatt tgatagcaaa gggattttcc
     1921 gccaccagtg ggcataacaa ccaggcaatc gcgaccggaa agcacagtgt cgataatttc
     1981 ttcctggccg gggcgaaact gttggtagcc aaaggtttct tgtaaaacct gtttagctcc
     2041 ggactccaga ttcaacactt ccgcctgcgc cacattcacc ccgattgcct gaaataaaaa
     2101 caggcgctat tttcagcgcc tgcgcgagaa actgcaatgc ttttagtctc tgagagggta
     2161 atcctggatg ctggattcca gatttgccaa ccactcaacc gttcatcaaa acaagtcgtt
     2221 tagcataacc cctacaccga cacgggtctg gttgaagtta tagtcgatga gcgattctcc
     2281 atagccgctg taaacctgag tataaaggcg cacatgtttg gtgatcgggt aacttaagcc
     2341 taactccgcg ccgccgtagc cggtgttcca gttgtactgt cctttcgcac tgagcaccgc
     2401 atcaccgagg tgatagccga ttttaagctg gtagtaaccc atatatttgg tgatatccgg
     2461 gttatcgtca gtattaccca ccacatacca cggcttcact tctaccagcc agttaccgtt
     2521 ttctgccatc aggcgagtat aaaggcggtt ccagctgcgg gaggtcgggt ctgaacgccc
     2581 gttagagtcg tggttatacc ccatctccac atcgcgcagc gtccaacctg caaaacggta
     2641 atcggtggca aaaccgagga acaattgcgg ttcgtagttg gtttcacgaa acggtgaaga
     2701 ctcttcgcta ttggacagtt gccaccagga tttttgcgta taagacgcac ccaacaccga
     2761 gtttgggcct aaaatcccac gccacagcgg aaatgccagg ctcaactgaa actttacttc
     2821 atccttacgc gcattttccg cccagtcgta actggcaatc gcttctttat tcagatcgct
     2881 ggtttgggtg tagatgaggt agttggtgtc ataaggatag agcgtgaacg gattgtcatg
     2941 ctcctgcagc atattggcga taatactgcc acgcaccgct ggcgcgtcat gcacctcttt
     3001 caccgttgcc tcttgtgcat atactgccat aggcaacata aacaccggca acaaccagcc
     3061 ctgcagagtc cgcatcggtc gtgttctccg taacgaatta ttgataaggt gaatgttttt
     3121 ctgcgggcta ttttacatac ttaactccat tgccgttagt tatcccttcc taaagtagaa
     3181 accaacaaca aagaagcata aaatcaacat ttcattaacc aatgaagtgt aaggatgata
     3241 tgtctgccgt actgaccgct gaacaagccc tgaaattagt gggtgagatg tttgtttatc
     3301 acatgccatt taaccgcgca ttggggatgg aactggagcg ttacgaaaaa gagttcgcac
     3361 agctggcctt taaaaatcag ccgatgatgg tgggtaactg ggcgcaaagc attttgcacg
     3421 gcggagtcat tgcgtcggcg ctggatgtcg ccgccggtct ggtgtgcgtg ggaagtacct
     3481 taacccgcca cgaaaccatc agtgaagatg aactacgcca gcggctatcg cggatgggga
     3541 ccattgatct tcgcgttgat tatctgcgcc caggcagggg cgagcgtttt actgctacca
     3601 gtagcctgtt gcgtgcaggc aataaagtcg ccgtcgcccg cgttgaatta cacaatgaag
     3661 aacagcttta tattgccagt gccaccgcca cctatatggt aggttgaaga cataaaagca
     3721 ggtaaactgc tgttaccaat taaaaatgag tattcccgat ggatgcaaaa caaacgcggc
     3781 agggcgtatt actcgctctt gccgcttatt ttatttgggg tattgcgcca gcatacttca
     3841 agctgattta ctacgtgccc gccgatgaga tcctgacgca tcgcgtgatc tggtcgtttt
     3901 tctttatggt ggtgctgatg agcatttgcc gccagtggtc ctatttaaaa acgctgattc
     3961 agacgccaca gaaaattttt atgctggcag tctctgccgt gctgattggt ggcaactggc
     4021 tactgtttat ctgggcggtg aacaatcacc atatgctgga agcgagcctt ggttacttta
     4081 ttaacccgct ggtgaacatt gtgctgggga tgattttcct cggcgagcga ttccgccgga
     4141 tgcaatggct ggcggtgatt ctggcgatat gtggcgtgtt ggtccagctg tggacttttg
     4201 gttcgctacc tattatcgcg ctggggctgg catttagttt tgccttctac ggcctggtgc
     4261 gcaagaagat tgcggtagaa gcacaaaccg gcatgttaat tgaaaccatg tggctgctgc
     4321 ccgtggcggc gatttacctg tttgctattg ccgacagttc aaccagccat atggggcaaa
     4381 acccgatgtc gctgaattta ctgctgatcg ccgccggtat tgtcactacc gtaccgctgt
     4441 tgtgttttac tgccgctgcc acgcgcctgc gtctctcaac gttaggcttt tttcagtaca
     4501 ttggcccgac gctgatgttc ctgctggctg tgacgtttta tggtgaaaaa ccgggtgccg
     4561 ataagatggt gactttcacc tttatttggg tggcgctggc aatttttgtg atggacgcga
     4621 tttatacgca gcgtagaacg tccaaataaa aaataaatat attctgaaaa aatatgccga
     4681 ctcttgtatg agagtgggca tattgccatg ctgacaggag actcacacaa ggatgtaaaa
     4741 tttatgttac gtatgtttat cccaacatct aacggtaag
//
LOCUS       DADLZM010000206         4735 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLZM010000206 DADLZM010000000
VERSION     DADLZM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.210"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..181
                     /locus_tag="JNY73_003864"
     CDS             <1..181
                     /locus_tag="JNY73_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8049689.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
     gene            524..1240
                     /locus_tag="JNY73_003865"
     CDS             524..1240
                     /locus_tag="JNY73_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8049690.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1696..1771)
                     /locus_tag="JNY73_003866"
     tRNA            complement(1696..1771)
                     /locus_tag="JNY73_003866"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1736..1738),aa:Asn,seq:gtt)
     gene            complement(1872..3499)
                     /gene="yeeO"
                     /locus_tag="JNY73_003867"
                     /pseudo
     CDS             complement(1872..3499)
                     /gene="yeeO"
                     /locus_tag="JNY73_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3504..3579
                     /locus_tag="JNY73_003868"
     tRNA            3504..3579
                     /locus_tag="JNY73_003868"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Asn,seq:gtt)
     gene            complement(3617..4567)
                     /gene="cbl"
                     /locus_tag="JNY73_003869"
     CDS             complement(3617..4567)
                     /gene="cbl"
                     /locus_tag="JNY73_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY8049691.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4669..>4735)
                     /locus_tag="JNY73_003870"
     CDS             complement(4669..>4735)
                     /locus_tag="JNY73_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen assimilation regulator"
                     /protein_id="HAY8049692.1"
                     /translation="ELLMSVISSPVMEKRQWQLVS"
BASE COUNT         1438 a         1110 c          970 g         1217 t
ORIGIN      
        1 tgtttcagat aaaccgttac atggtgagat taagcttccc ggccaggcta accgttttta
       61 tgaaggcgct atttccgaac acctgcaaat tggcattcgg gcgatcgatt tgctgcgcgc
      121 agaaggcgac cgactgcatt cacgtaaatt acgaaccttt aatgagccgc cgttccgata
      181 ataagttatg aaccttctta tcagaaatag atgtgaagga ttaagtaaga catctggcaa
      241 atagcctgca ataaacgcgg gctgcttgct tccttgggca gatgcgagtt ttattatcgt
      301 cttaatgatt tccacatatt aaaagcaagt atgctttcaa aacacaatta taaaaaatcc
      361 cgccaacaac attagttttt ataaaattaa atataagatt atggctttag aatattttta
      421 tttctaatag acgagatttt tcctgttatg atataatatg ccgaattaac acatgttaac
      481 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
      541 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      601 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
      661 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      721 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
      781 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
      841 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      901 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
      961 tatttttgaa attttgcttg aagctgaagt tgatgttcgt gatgtaactg aagaagaagg
     1021 taatattgtt atttatactg aacctgctga ccttcataaa ggaatcgcgg cgctaaaagc
     1081 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
     1141 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
     1201 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatttta aagaaatctg
     1261 tctttatggc agatttcttt aatctcatat aattcttata aaaaatataa tgtttaactc
     1321 gtcatattgc ttataaaaaa tccccgttcc cagagaaata atatttatta aaatggcagg
     1381 tcttcttttt ctgattaccg aagacaaagt ggcaattacg tatagtttcc cgataaagac
     1441 gcgacagcta catcccgcat aaggcatttt tctctttatc tttgtacggt acttcatgga
     1501 acagaatttt tgaccttgtg aatcgtgatg tctgttgggg aggggcaatt tgctcactga
     1561 agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggc cactttttta
     1621 caaagtggcc gggtaaaaat aatttttatt ttttaactgt cctgagtctc atagagatgt
     1681 ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag
     1741 tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat
     1801 gcccacccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg
     1861 cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg ctcggatcga
     1921 gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca
     1981 gcccagtagg caaacattcc catccagaca ccaaccacac cccagccaag catgattccc
     2041 tgcacataac cgactacaac ccggcaaccc cacatgctca acatagaaac ccacatggcg
     2101 taacgggcat cacgagcacc cttaaatcca gcgggtagca cccatgaggc ggaccaaata
     2161 ggcataaata aagcatttag ctaaatcaga atcacaacga cgtgtttaac ctgtggatcc
     2221 tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt
     2281 aatccaagag tggacagcca gaacacatgc cgcaactgaa tctctgcttg cgctatctgc
     2341 cctaccccca accttcggcc tgtaatgatc gtagaagcag aaccgagcgc acttccgggt
     2401 aagttgataa gagccgcaat tgagaacgcg ataaaatttc cggcaataac gctggtcccc
     2461 atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca
     2521 ctcgctggaa taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa
     2581 tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt
     2641 gcaactgcgc caatataacg agaaatggtt aaacccaacc cagccccgac caggagaaaa
     2701 ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca
     2761 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg
     2821 cagcggctgg ataactgagt acggtcagct ccagataagt caacgccagt gctttaactt
     2881 ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaattagcg
     2941 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
     3001 ctcgtcgtcg atcccgctta ccgagactaa atgctacaac gacagtagta ccaagatcga
     3061 tagctgcaaa aaaagccata atgaccatat tgaagctgtc cgccaacccc acgccggcca
     3121 tcgcatcttt tcccagccag ctgaccagaa aagtgctcag gacccccatc aacaggacac
     3181 aggcgttctc catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt
     3241 tgtagctctt gcgtttagag tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
     3301 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat gatgagggag
     3361 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
     3421 atagaaacgg caaacagttg ggaaatttaa aaatcgggtt tgacaaaata tttttcgccg
     3481 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
     3541 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
     3601 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
     3661 ggcgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag cgccaaacat
     3721 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     3781 gccgcgtatc caggcggatt aaattctctt cctcttgttc gccgctggat tgttcggcaa
     3841 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgtgcac
     3901 ttaatacaat atctgccggc aaacctttgc gggtaaatgc gtcatcagtg cgtgagcgcc
     3961 ccgtaattcc ctgtcggtaa gtaattaacg gccacttcgc tattgattcc agcgtcaatg
     4021 gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc caacaaaacc
     4081 acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     4141 cttcgccatt ttgcaacaac gtcgcaattt cctgtggcga accctggatt agctcgagcc
     4201 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     4261 gagtatgcgt cgttgcaata gtgagaacac cagacgtatc gttggtaaac aggtctgcaa
     4321 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     4381 ccggttcagt catccccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt
     4441 catcctccag ttcccgaata tgacggctaa cgcctgactg tgaggtaaaa agcatattcg
     4501 caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga
     4561 aattcacggt aaactccggg cagttcagat atcccgttat tgttaaagtc taatgcccgg
     4621 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     4681 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actct
//
LOCUS       DADLZM010000207         4611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLZM010000207 DADLZM010000000
VERSION     DADLZM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.211"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..198
                     /locus_tag="JNY73_003871"
     CDS             <1..198
                     /locus_tag="JNY73_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY8049693.1"
                     /translation="FLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQLIGIGVLGE
                     YIGRIYLETKSRPRYLIKSRK"
     gene            211..1476
                     /locus_tag="JNY73_003872"
     CDS             211..1476
                     /locus_tag="JNY73_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY8049694.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(1543..2166)
                     /locus_tag="JNY73_003873"
     CDS             complement(1543..2166)
                     /locus_tag="JNY73_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049695.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(2908..3255)
                     /locus_tag="JNY73_003874"
                     /pseudo
     CDS             complement(2908..3255)
                     /locus_tag="JNY73_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            3377..3637
                     /locus_tag="JNY73_003875"
     CDS             3377..3637
                     /locus_tag="JNY73_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HAY8049696.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(3655..>4611)
                     /locus_tag="JNY73_003876"
     CDS             complement(3655..>4611)
                     /locus_tag="JNY73_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049697.1"
                     /translation="RRLVGDALHKNSGPYKDADRAALSRLYGSLADDQKAALSETNAL
                     RDFEVAQRLVQMRKSMEEQMINLTGRTLNGDVSRKATTALQAMSKGDAKGFRELMQNT
                     PSRKLRTELLGTGLRDMLSNGKRGADFNPAGFADWYQNMLANGQMRNLARHLPKETMS
                     GLNDVYKVAKAIKDAKSYEITTGRLNEFVKRFNRVTAANEFVANHAQRIGTAVGSTVS
                     GPFSAVGAVAGSEIGAKVASKIRAMGGAESIESAEKLISSPEFQKAARLAVKQAPESI
                     VDTTVRRSSAWRSFYNSLPESDKKTISRLGIMYWMNSDDNQK"
BASE COUNT         1298 a         1006 c          884 g         1423 t
ORIGIN      
        1 tttttatatg gtgcatggat gattatagac accattgtct ttggtaaccc agtacgcggg
       61 tatccctcca tgcttgtatc aatacttttc ctgggtggag tgcaactgat cgggattggt
      121 gttctcggtg aatatatagg tagaatttat ttagaaacaa aatcaagacc acggtactta
      181 attaaaagca gaaaataaca ggatgatctt atgaagatat tcataatgcg caattggcac
      241 aagatatcaa tttttatact tgcatttact ttgatttggc ttaggcgcat tgacattttg
      301 acaaatgccc agttttgggc tgaggatgca gttttttggt ataaagatgc atacgaacaa
      361 ggttttttca gttcactcat aaccccaaga aatggttatt ttcaaacagt atcaacactc
      421 attgttggtg cgacgacatt tattaatcca atttacgcac cattattatc aaactttttt
      481 ggaataataa ttagagctat aattatatgg ttcttattta ccgatagatt taaattccta
      541 agcaccacat caaaaatctt catctctgta tatttaattt gcatgcccgg gcttgatgaa
      601 gtccaagcca atataacaaa tgctcactgg tatttatcat tgtatgttgc aatgataata
      661 atatctgaag aatccaagtc aaaattatgg aaagcacatg atttattttt tatagtatta
      721 tctggattga gcggtccatt tattatattc ataattgcat catctgtttt taaatattta
      781 cacttatcaa aaggagtaat aagcataaga ggtttatact tattttatac tcgactgcca
      841 tatttggcaa tgatcttgtg tgggctgata caggcaacat caataattct tacatttaat
      901 ggaacaagat cacatgctcc attaggattc agttttgatg ttatgtcatc tataatatca
      961 tctaatgtat ttcttttttc atttgtccct tggaatatcg ctaatgctgg atgggataat
     1021 caccttttat cgtacacact atcaacttta gtaattacat tatgtgtttt catttatatt
     1081 aaaggaaatt ggcaaatgaa agtgtttgcc acccttccaa tacttattgt cgcattttct
     1141 atggcaaaac ctcaactggc agattctgtt cctcaactgc caacacttgc aaccggaaac
     1201 gggtcaaggt attttgtaaa tattcatatt gcaatttttt cattaatatg cgtttgtttg
     1261 tttcaatgca taaagaataa aaccttaaaa atattcttta aaatctatgt ttcagtcatc
     1321 cttattgcaa tgataaaatt aaactttttt ataacccctc ttcctgacat gaactggtct
     1381 caaggagcag aattaataaa taaagcaaaa catggagaag ccgtctcatt tacagtgctc
     1441 cctccatggt taactcttga tttaataaaa aaataaaact tcacattaat aaataacaat
     1501 gaatccgcct ccctacgtat tcccgtaggg aggctgtcgt gtttatagta gcctggtaat
     1561 gctaaatgca aacgaactgg agattcctgc tccgctaagc gtattgcgca gggtcaccct
     1621 aaatgtatct acaccaatat aagtaacatc acaactaact gaatcaggcg cagatatagg
     1681 tgttatctga accacataac cattagtagc tgctgcgagc ggatgagaaa acgttacatc
     1741 atacacacca gtagcatgcg cgttaacgga tgaaacaccc ggcgcaatgt ctacactacc
     1801 attggcgtta attctccctg agcgcgtaac tgactctgca gaaaccgatg tggccttact
     1861 ggttatttcc ataaaggtgc aaccaatatc gatcaattcg caattgttcc ctgttatcca
     1921 gtatggtcga tctgcacttg gctgataatc ccctgcgctc tggaacgttg tgccattgag
     1981 taagactata acttttcccc cacctgagct tgttacttgt atattcgtgt gcgtgtagcg
     2041 ttcactgctc acgcgatgaa aatttccacc gtttataact accgttacag gtcgcgtccc
     2101 agtattatct atagcaaggt ctgccccccc ggcattagcc tcgaagtatg ggttgtttaa
     2161 caccaaagca cacgaaccgt taaggccatt tatggctaat tgcgcaccac ctgtaccgga
     2221 atccccctga gtaccatttc cttcacaagt caggctgttg atagtagttc ctgcccccca
     2281 tctatcacca agtatacccc atcgtgtatt ctggcttgca gttaatcggt taactgtaat
     2341 ggcattaagc tctgaccctt cactggagtt aagtgcgcgc cagccaatac ggttaccctg
     2401 taagtacaca ttatctaact ctgacgttaa caccgagtct gtacgcaacc cttcgccggt
     2461 agtgtgccca taagacacaa tgttctctaa tttagtgtat gctttattgg ttacgagaag
     2521 accgtaagac gatcccgaac ctttaagtgt aatgtccccc agataatcat aggcgtgtac
     2581 acgttgggtt gccccgactg tatcaccgag catctttaag ttgaaatccg aggcatggca
     2641 gttaaatatc gtttctgcca tattttctcc ggaataatga gcacggactc ctgtcgaacc
     2701 aataaattta gcgtcatagt tgccgtaatc aagaacgagt ggtgacgcga tatcgtacac
     2761 cccttgcttt atttttaatg ttccaccgaa aggccacgat gtaagcgggt cttgatatat
     2821 ttcatttttt attgcaaata gctcctcttg gagatttttg ccgctattag tgccaatgtg
     2881 agcagctcct tcatctgatt gaagtaactg ctttaactga tcaggatcgt acttcagcac
     2941 attctgaaaa tagaactgct gcgaaccata cgcatcatat acagccattg aatggccttg
     3001 ctcagttaca aatttggcaa tctgcccgtt gtacacagga tatccggcag cattgataac
     3061 aattggctga gaaacaggga cgtgagatcc gtcttcattc tctacataaa cctgaatctg
     3121 gttttcagga tttaccgggt cagtgtcaat ttttccgata taaattttgc cattggatac
     3181 ggctttaaaa gaacgcgcca tagtgaagag ttgcgaaggc atgctgacca caacatttgc
     3241 ggcgatatct gacatttcat tgctccagac gaatgatatg atgcaatcat gatgtgattg
     3301 cataccgaaa tggcactatt gaatatttat ccagtaggtt acgatgccat tccacccaac
     3361 tggtgaggca tcaaggatgt acagcaaata cgacgaggca cagtttcact tgagacttcc
     3421 gcatgaactc cacgcgaaaa ttaaacagtg tgcgaagatg aataacagtt cgctgaactc
     3481 agagataatt gcagcgattg aagaatcatt ggctaaacaa agctctgcat cagtttacat
     3541 tgacgatgca gagcgtatgg cagaacaaca atctgatatg gttaagaaaa ttgtctttga
     3601 tacgctcaag gagctatata aaaaagacag caactaacca tccgtggctt tccgttactt
     3661 ctggttatca tcactgttca tccagtacat gatgcctagc cttgatatgg ttttcttatc
     3721 tgattctgga agtgagttgt aaaacgagcg ccaagcagaa gagcgtctta cagttgtatc
     3781 aacgatgctt tctggtgctt gttttactgc cagccttgct gctttctgga attctggtga
     3841 gctaattagc ttttctgcag attcaattga ttcagcgccg cccatcgccc tgattttgct
     3901 ggcgactttt gccccaattt ctgacccagc aacagcacct actgcactga acggtcctga
     3961 cacagttgaa ccaaccgcag tgccaatgcg ttgggcatgg ttagcaacaa attcatttgc
     4021 cgcagtgacg cgattaaacc gtttgacgaa ctcgtttagt cttcctgtag ttatctcgta
     4081 agattttgca tctttgatag cctttgcgac cttatataca tcgttcagac ctgacatagt
     4141 ctcttttggt aaatgtctgg caagattgcg catctgcccg tttgctaaca tgttttgata
     4201 ccagtcagca aaccctgcag gattaaaatc agcgccacgt tttccgttcg aaagcatatc
     4261 ccgaagacct gttcccagta gctcggttct tagcttcctg gacggcgtgt tttgcatcaa
     4321 ttcacgaaat cctttggcat cgcctttcga cattgcctgt agtgctgtag ttgctttgcg
     4381 agaaacatca ccgttcagcg ttctgccagt tagattaatc atttgctctt ccatgctttt
     4441 tcgcatctga acaagcctct gagcaacttc aaaatcacgt aatgcatttg tctctgacag
     4501 cgccgccttt tgatcatcgg cgagcgaacc gtaaagcctc gataaagcag cgcgatcagc
     4561 atctttatat ggtccagaat tcttatgaag tgcatcacca acaagtcgtc g
//
LOCUS       DADLZM010000208         4606 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLZM010000208 DADLZM010000000
VERSION     DADLZM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.212"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            314..1222
                     /locus_tag="JNY73_003877"
                     /pseudo
     CDS             314..1222
                     /locus_tag="JNY73_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
     gene            1222..1956
                     /locus_tag="JNY73_003878"
     CDS             1222..1956
                     /locus_tag="JNY73_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAY8049698.1"
                     /translation="MVNDLTYKKLQSTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRNERCNNMRTKQKV"
     gene            2424..3185
                     /locus_tag="JNY73_003879"
                     /pseudo
     CDS             2424..3185
                     /locus_tag="JNY73_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            3229..4308
                     /locus_tag="JNY73_003880"
     CDS             3229..4308
                     /locus_tag="JNY73_003880"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8049699.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMGYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQHFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4305..4592
                     /locus_tag="JNY73_003881"
     CDS             4305..4592
                     /locus_tag="JNY73_003881"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY8049700.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQLLDLPLYFQTSVIT"
BASE COUNT         1262 a          884 c         1126 g         1334 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtgaccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagtgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt acaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggaatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttaca aatataggtg atttttttat
     2221 tgtaggcttc tgaaggctat tataactgtg cgctaaatta atgcgttgaa taaaagaaat
     2281 aacaaaatta agaaaagcca tatttacgat atgggcaaca taaatataat ctaaaaaaga
     2341 gtaaggcgtt gtaggttggt tccgcgttca ttaccttgtt agtgaacgcg gatttatcag
     2401 ttgtcgaaat ggtttttata ataatgaaaa attttaatgt actaaccaaa gtcgtatttt
     2461 gcttgtcgtt tttatcaagc gcatttccca cctatgctgc ggatgatgtc aaagtgactc
     2521 aaaacacttc agtttttaaa ctgaagctta gtgcaacgcg attaatctat gaacaagggt
     2581 cggatggcgc agcgataact gtgacaaatc cgcaggattg gccgatgctt gtgtagagta
     2641 aagtctttga agaagataaa gtaactgcag cgccctttat tgtcactccg ccactgttca
     2701 ggcttaatcc aggccagcaa aataaaatgt gggttgtgca gactgcgcca gctttacaag
     2761 acagggaaaa attacactgg ctatgcgcca ccggcattcc gcctaaaaag agtgatgttt
     2821 gggccgagaa agataaagaa aaaattgctg aaaatgtgaa cctgaacatc caggttaacg
     2881 tacatacctg cataaaactt attaccaggc catctggtat tgaaggcaag atggttaatg
     2941 cgagtgagaa gttatcgtgg aaaaagcagg gtgataaaat tgtcgcggtc aatcagtcgc
     3001 cgttctacat caacctttct tctttacagg ttggcggtag caagattgag aaactagatt
     3061 atattgcgcc gttttcgcaa catgagtttg ttatgccaaa aactagcggc caggatatta
     3121 aatggtctgt gattactgat gaaggtggtg agagtcgttt gtttacatct catttgcaat
     3181 cataatgtat tacattttta ttgaatattt ttatgccatg catattccat gttttatcga
     3241 ctgtcattaa ttttgataat ggccttactt gcgggtcagt taagtgccca ggagtggtca
     3301 tttgattctt cacagttgga aggaaacgtt agtgcggaca ctgtggcaat gtttaatcag
     3361 ggagagcaac tccccggaaa ttatcgtgtg gaaatttatc ttaacggtga aaaagttgat
     3421 gttggggagt ttccctttca tcgtccggaa tcccctgaag aaaaagagct tgttccatgt
     3481 ttaaccgttg atgaccttat ccactatggc attaaaattg ataaaagcag cagtgatact
     3541 gacaataaaa agaatcagtg ttttaaatgg aactctattg aaggtttgaa agtaaattat
     3601 gattttgata gccagcgcgt acagataacc gttccacaac tgtatctgca agataaaaaa
     3661 agtagtttag caccagtgtc gttatggaat gagggtgtag ctgcattccg catggggtat
     3721 cagacaaata ttgatatttc caaacaaaat gataatcaat ctaccacgcg taattctcgt
     3781 tatggcaggt tcactccagg gtttaatctt ggtgcctgga ggtttcgtag ttcagtgaca
     3841 tggagcaagg aacttggcca gtcagaaaga tggcaaaggg gttacatgtg gtttgagcga
     3901 ggcataaatg cgattaaaag ccgattaacg cttggagaat cttatacgtc gtctgaggtg
     3961 tttgacagca taccttttcg cggcgggatg ttagcgaccg acgatgcgat gactccgccg
     4021 gaggacagct actacacacc tgtggtgcat ggaatagcac aaagtgaggc gcaggttatt
     4081 attaaacaaa atgggcaaat tatttttacc cgttctgtgc cgccagggcc ttttgcgctg
     4141 gataatttac caacgctggc cgttggcggg gagcttgatg ttacagtgag agagagtaat
     4201 ggcgaggagc agcatttttc cgtgccattt caaacgccgg ccatcgctct gcatgaagga
     4261 tatttcaaat atagtgtgat gggggggaat ataaagaaga aggtataaaa agctcccctg
     4321 tctccgtggg cgaaatcacg gcgatgtacg ggcttcctta tggcgtcacg gtgtatggag
     4381 ggattcaaag cgccactcat tttaatgcaa tatctacggg tattggcatt tctctggggc
     4441 tgttagggag tttatcgaca gatattacgc gttcgattgc gaatttatat tatgggaata
     4501 aatatcgtat tcgttatagt aaatccatta gtgattttgg tactcaattg ttggatttgc
     4561 ccctatattt ccagacatct gttatcactt aacccattac aagccc
//
LOCUS       DADLZM010000209         4549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLZM010000209 DADLZM010000000
VERSION     DADLZM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.213"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            132..575
                     /locus_tag="JNY73_003882"
     CDS             132..575
                     /locus_tag="JNY73_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8049701.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /locus_tag="JNY73_003883"
                     /pseudo
     CDS             811..1630
                     /locus_tag="JNY73_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNY73_003884"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNY73_003884"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY8049702.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNY73_003885"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNY73_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY8049703.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNY73_003886"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNY73_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8049704.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKANEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /locus_tag="JNY73_003887"
     CDS             complement(4321..4470)
                     /locus_tag="JNY73_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY8049705.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
BASE COUNT         1233 a         1188 c         1084 g         1044 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 actacttgta atcaattgat tataaaggat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgttggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagggtaatg ctgccaactt actgattta
//
LOCUS       DADLZM010000210         4508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLZM010000210 DADLZM010000000
VERSION     DADLZM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.214"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            368..2020
                     /locus_tag="JNY73_003888"
     CDS             368..2020
                     /locus_tag="JNY73_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY8049706.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JNY73_003889"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JNY73_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2280..2393)
                     /locus_tag="JNY73_003890"
                     /pseudo
     CDS             complement(2280..2393)
                     /locus_tag="JNY73_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2853)
                     /locus_tag="JNY73_003891"
     CDS             complement(2527..2853)
                     /locus_tag="JNY73_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY8049707.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
     gene            complement(3138..3790)
                     /locus_tag="JNY73_003892"
                     /pseudo
     CDS             complement(3138..3790)
                     /locus_tag="JNY73_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
BASE COUNT         1198 a         1089 c         1038 g         1183 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctttcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaacacggg ccagccagtg gctggcccgt
     3241 cgtcggtggc tcctaagtcg cttgtgttta cggggcagcc ataacacgag tttgcgatcg
     3301 atttgcctca gcgtcagata cagtgctgat ttataaaaag caccgtagta ctttatccag
     3361 cctctgattt tcgcattgaa catatgcgcc agctcgtaca aggacttgtc actgcgattc
     3421 tcctcacctc ctgacggatt gcttttcctg ctctggcact catcgccgga ctgaagttaa
     3481 cgaaggtctt accccagcga ttcatcgaca gacgcggccg gaatgtatag cccagaaagt
     3541 tgaagctcgt ttccgggtag tccccccgtc ggtcagcatc cttacagtaa actattcgtg
     3601 ttttttgcgg atgcagctgc agcccgcatt gcgcaagccg ctgcctcagt ccacttatca
     3661 gagcgtcagc ctgccactgg ctgtgacagt ggcatacaac atcatcggcg tacctttcga
     3721 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3781 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3841 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3901 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaaaga
     3961 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     4021 caatcgcctg gtgtgaggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     4081 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     4141 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     4201 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     4261 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     4321 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     4381 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     4441 ggggcaagcc ctttgcttcg gacccattac ggtccttcac cgctactacg gtttactccg
     4501 tccctgta
//
LOCUS       DADLZM010000211         4474 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLZM010000211 DADLZM010000000
VERSION     DADLZM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.215"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..791
                     /locus_tag="JNY73_003893"
     CDS             <1..791
                     /locus_tag="JNY73_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY8049708.1"
                     /translation="AAKRSADGELLLATRNRLSSVVPLSPDAWQMLNAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLS
                     LPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAE
                     TAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLT
                     AVGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            916..1377
                     /locus_tag="JNY73_003894"
     CDS             916..1377
                     /locus_tag="JNY73_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY8049709.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(1418..1888)
                     /locus_tag="JNY73_003895"
     CDS             complement(1418..1888)
                     /locus_tag="JNY73_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY8049710.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1935..2654)
                     /gene="yehT"
                     /locus_tag="JNY73_003896"
     CDS             complement(1935..2654)
                     /gene="yehT"
                     /locus_tag="JNY73_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY8049711.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(2651..4336)
                     /gene="btsS"
                     /locus_tag="JNY73_003897"
     CDS             complement(2651..4336)
                     /gene="btsS"
                     /locus_tag="JNY73_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY8049712.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT         1146 a         1155 c         1057 g         1116 t
ORIGIN      
        1 aagcagcaaa acgcagtgcc gatggcgagc tgctgttagc tacccgcaac cgcttaagca
       61 gcgttgtacc gctgtcgcct gatgcctggc aaatgttgaa cgcgccgtta cgccagccgg
      121 gcattgtggc tttgcgggaa tatttacgcc agcgtccccc cgcctgcata cggcctctta
      181 atcaggtcga taacttattt attctgccgg tcgctgagtg tatttcgctc ggttgggata
      241 gcagccgcca gacgctggat gcgcaggtaa tcagcggcga aggtgaagat aatctgctga
      301 cgttatcact gccagcctca gccagcgcgc cttatgccat tgaacgcatg gcggcgcttt
      361 tgcaacaaac agacgacccc gtgtgtctgg tttctggctt tgtcagtttt gttgatgggc
      421 aattgacact ggaaccacag gtaatgatga caaagactcg tgcctgggcg ctggacgcag
      481 aaactgcgcc tgtggtggta tcactacctt ctgccagcgt tctgcctgtg ccgtctactg
      541 ctcatcagtt gctgatgcgc tgccaggcgt tacttattca actgctccat aacggctggc
      601 gctatcagga acagagcgct attagtcagg cagagttgct ggcgaatgac ctcaccgcgg
      661 tgggttttta tcggctggca catgtgttgg cacaatttcg taatacagaa agcgaggcac
      721 gggtagaagc aatgaataac ggtgttttgc tttgcgaaca attattcccc atgcttcagc
      781 aacaaggatg aaatagtgct ttttactaag agttctactc cagttccgga ctgctcacgc
      841 cacggtatta ggcatatcct atatagcccc tggtgagagt caccagtttc ttgattaaat
      901 aaaatggagt tttacatgaa ggctttcaat aagctgtttt ccctcgttgt tgcatctgtt
      961 ctggttttct ctcttgctgg ctgcggtgac aaagaagaat cgaagaaatt cagcgccaat
     1021 ctgaacggca ctgaaattgc cattacctat gtctacaaag gtgacaaggt gcttaagcaa
     1081 tcttctgaaa ccaaaattca atttgcctcc attggtgcaa ccaccaaaga agatgctgcc
     1141 aagacacttg agccgttaag cgccaaatac aaaaacatcg cgggtgttga agaaaaatca
     1201 acctataccg atacctacgc gcaggaaaac gtgactatcg atatggaaaa agtggatttt
     1261 aaagccctgc agggtatttc aggaatcaac gtttctgctg aagatgccaa aaaaggtatc
     1321 actatggcgc aaatggaact ggtgatgaaa gccgctggtt ttaaagaagt gaaataatct
     1381 gtcggcggcc atgtttcgca tggccgccat acccgtctta gcttttcttc acatgctggc
     1441 gcgctgccag tccacgcaga aaataacgta aaaattgatc gccgcattcg cggaagtttt
     1501 tatgatccgg tgcgcgcatc atcgctgtaa tttccggcat cgaaacgcgg aactgctgtt
     1561 cggtgaggat agccagaatg tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca
     1621 gcacgatgtt gttattaatg cgacgttccg gctccagtgc cggagcagac tcatccttgc
     1681 cgcgtttttc ataaatcagg ccattgagga atgacgacaa aacaatgtcc ggacaacgct
     1741 gaaaaccctc ttcgtcttct ttacgtagcc agacggcgat ctgttccgcg gtggcttcga
     1801 cattatccag cgccagaata cgcaccaggt cattattatt ggctttcaaa atgtagcgca
     1861 cgctgcgcag aatatcgtta cttatcatga ggccttcagg tgttgatgag gcaaaaagcc
     1921 attttagcag tcttttacag gccaatcgcc tcttttaagc ttttcagata acggcggctg
     1981 accggcacgg ttaagccatt acgcaaaatc aactcggcct ggccgttatc ttccagacga
     2041 atctcctgta aatgcgcgag gttaaccaga tactgacgat ggcagcgcag tagtggtgta
     2101 cgactttcca gggtacgtaa tgtcaattcg gtaaagccct ctttcccttc gtggctggta
     2161 acgtagacac cgctcatccg actgctgaca aatgccacat ctttcatttg cagcaaataa
     2221 atccgactat gccccgtaca agggataaat ttcagcgcct gttgattttc cggtaacagc
     2281 gaaacatcct gcttgctgcg ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct
     2341 tcatcaattg gcttcagcag ataatcaaag gcatgttctt caaaggcttt aatagcgtat
     2401 tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt ccgggtcaag catccccacc
     2461 atttccagac cactgatgcg cggcatctgg atatcgagaa acagcacatc cgggcgcagt
     2521 ttatgcaccg cgccgatccc ttccacggcg tttgaacact ctccaacgat ttcaatatcg
     2581 ctctgctcct gcaaaaatat acgcaggttc tcccgtgcta acggttcatc atcgacaatt
     2641 aagactttaa tcatgcctcg tccctccatg gtagtcgtaa cgttattcgg gtgtaactat
     2701 caggctcaca ggcgacgctt attccatagt catcgccaaa ccgttcacgt aaacgcttat
     2761 ccaccagatt catccccagc ccactggcat tggttaccgg ttgatacaaa ccggcattgt
     2821 cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc gctgattgcc actcgccctg
     2881 tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc cactatcggt tgcagggtaa
     2941 acgcgggcaa ttgctgctgg gataattctt gcggaatagc aatgttgacc tgcaaccgcg
     3001 actggaagcg cgccttttca atttgcagat aagcgttcac atgttcaatt tcgtcggcga
     3061 gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa aaaagtggaa agatactgca
     3121 ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat
     3181 taaacaaaaa atgaggattc acttgggcgt gaagcagttt gatttctgac tgggtgagca
     3241 tcgctttttg ccgctcatat tgcccggcaa gaatctgcgc cgaaagcagt tgcgcaatcc
     3301 cctcgcccag cgtgcggttt attgaactga ataaacggtt tttggcttca tacaatttga
     3361 tggtgcccat cacgcgctga ttttcgccac gcaacggaat taccagagtc gaccccagtt
     3421 tgcattgcgg atgcaaagag caacgataag gtacttcgtt gccatcagcg tagaccactt
     3481 caccggtttc aatcgctttt aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt
     3541 ggtcgtcacc aattccggta aaggccagca atttctctcg atcggtaatc gcgactgcac
     3601 caatatccag ctcctgatac agcacctgag ccactttcat gctgttcact tcgttaaacc
     3661 cctgtcgcaa aatgccttcc gtcgaggctg ccactttcag cgcagtggca gaaaaagccg
     3721 aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat acgcataaac agcgccgcgc
     3781 cgacggtatt ggtgaccatc attggcgcag caatattact caccagacgc accgcatctt
     3841 cataaggtcg ggcgatcgca aggatgatca gcatttgcac catttcagcg acgaacgtga
     3901 cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc
     3961 tgtgtaccag gccgccgagc aatccttcaa cgatggttga gatcatgcaa cttagcgcgg
     4021 tcatgccccc catcgaatat cgatgtaagc cgccggtcag accaaccagc ccaccgacga
     4081 ccggaccgcc gagtaagccg cccattaccg cgcctatcgc acgggtattg gcaatagaat
     4141 cgtcaatgtg caacccaaac caggtgccca tgatgcagaa gatggaaaag acgatgtagc
     4201 agagaaattt atgcggcaga cgaaccgtga cctgcattaa cggtatgaat aatggcgttt
     4261 tactcattaa ccacgcaatg actaaaaaaa cgcacatctg ctgaagcagc agcaacacca
     4321 gattaaaatc gtacataccc gcaaaccaca cttcccttta aaacgcgtaa catacattgc
     4381 ctgcgtttaa ctttctttga actcttgcag aaaaatgaga attcgtgagt acgatcactc
     4441 aaaatcgcct ggcaaaaata aaatcaccct atag
//
LOCUS       DADLZM010000212         4460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.216, whole genome shotgun sequence.
ACCESSION   DADLZM010000212 DADLZM010000000
VERSION     DADLZM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.216"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..746)
                     /locus_tag="JNY73_003898"
     CDS             complement(<1..746)
                     /locus_tag="JNY73_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8049713.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGG"
     gene            complement(957..2354)
                     /gene="ccp"
                     /locus_tag="JNY73_003899"
     CDS             complement(957..2354)
                     /gene="ccp"
                     /locus_tag="JNY73_003899"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY8049714.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2759..4408
                     /gene="treF"
                     /locus_tag="JNY73_003900"
     CDS             2759..4408
                     /gene="treF"
                     /locus_tag="JNY73_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY8049715.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1132 a         1142 c         1057 g         1129 t
ORIGIN      
        1 aagccaccgc tggcagcgtc gatgtgcatg tcgatgtcga taccggtgtc ggcctggaat
       61 ttatccagcg catcgtgcag cggttgtggg aactcatagt taccggtgta ggtcacgccg
      121 aaagtcggca ccacgccgat ggtgttttcg tcacaggctt caatcatgcg tttcgggtcc
      181 ataaacaact gaccggggcg catagggatc tcacgcagct ccacatccca gtagcgggcg
      241 aatttatgcc agcagatttg taccggaccg cacaccaggt ttggtttatc cgttggtttg
      301 cctgcagctt ccatacgctt gcgccaacgc catttcatcg ccatcccgcc gagcatacag
      361 gcctcggaag aaccaatggt gttggtgcca acggcctgac catttttcgg cgcaggcgca
      421 tgccacagat cggcaaccat atttacgcaa cgcaggtcga tggctgcgga ttgcggatat
      481 tcttctttgt cgatccagtt tttattgatc gacaaatcca tcaatttatg gacgttttcg
      541 tcgtcccagg tctggcagaa agtggctagg ttctgacgag cgttgccatc aagatataat
      601 tcatcattga taatctggaa tgcgacatca tcgcgcattt cgtgcagcgg aaatcgtttt
      661 gactccgcga tagtagaaat ggcctttgcg ccaaaacgtg aatcgagtag ttctgagcgg
      721 aaatccgtta acagcttctg gtccatttcg aactccttaa atttatttga aggcaataaa
      781 aaagtaggat ttatccgcaa tggaagcaag gcattacagg cttaatttaa ataacaaaat
      841 cctaagcaga aaaacgactt tttataatat aaacatttaa catgataata ttaaaaacag
      901 actttattat cgtaataaaa atcgcccaaa cattcctgtt cgggcgatca aattaattat
      961 tgtttatcct gcatatacgg cgtgtacacc ccgttcagac tgtgcaggaa agctacgata
     1021 tcatccacat cctcctgcgg cagctctttg cctacctgat agcgcagcat cagtttcacc
     1081 gccccgtcca gcgtcggcac gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta
     1141 cgtaaaccgg gtactttctg acgcaattta tcacgctctt ctttagtcac attcatacga
     1201 ccaatatccg ccgccgtaat ttccccaaag ttaaagtctt ttttcagcac caacggttca
     1261 aaggaacgtc cgccgagaat aataccacca tgacaagttg cacatttatt atctttaaat
     1321 aattgatagc cttttttctg ttgcgccgtc agcgcatttt catctccacg caaccattta
     1381 tcaaatgggg aatccggcgt aattaatgtt ttctcaaatt cagcaatggc atcagtaata
     1441 ttttcgccac tgaaaccttg cggataaact tcgaggaact gcgctttaag ctgcggatct
     1501 ttttccagct tagcaataat ttcgtcccag gatttcgacg ccatttcaat cgggttcaac
     1561 ggcggtccac ctgcctgatc ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg
     1621 ttaaatactg agttaaatac cgtcggcgca ttaatcggcc caactgcgcc accaacacca
     1681 atcgatgttt ttctgccatc gacgcccccc gcattcaacg tatggcaatg agcgcatgaa
     1741 atggtgctat cagccgataa acggggatcg tgatacagcg caaagcccaa cgccactttt
     1801 tgcgcatcgg taggcagttt ttgcgggatg ggctgcaccg gttcattgcg atgctccgga
     1861 gcagtatcat tgctggcgta atattccgcg cgctgttttg caatccaggc cagtatttcc
     1921 gcccgctctt catcactcac cttacccgcc cagtgtagcg cggtataacg cgttggtggc
     1981 atagtttcat actgcatcac ccattcaatc ttattcagat cgctttgcga aacgggttta
     2041 tcagccagca gtgccgcacg cacggcctcg aggttaaaag atttatatcc aagcttaatg
     2101 tcgtaatcca tcaactgttt cgcgccagga atataataat aggcgggtaa ttctgccgaa
     2161 ggcgtgtggc aatagtcgca tcctttttcg cggagaaagc ctaaaacctt attattttca
     2221 ctgacagcag atgcctgaac atcggcctgt ttactgcgtt tattatcgtg gtaccacaca
     2281 taaccagata accctaaata gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt
     2341 gagaccattt tcattattct tccctcacgg tggtgagtta ttgtgaccca gaaaattttc
     2401 cagaatgctg caaacgtctc tgcgagacaa agccatcata aaaaacaggg agatacgctt
     2461 tttgatagga atcaatcaat tacatgaata taaactatca ataagatagc ctgtgcctat
     2521 tacccagatc aggaatggca tatcatgatt gcggcgagag caggattgaa tgttctgaaa
     2581 atgaaaaaca acaccaaatc cgtattttta taaattccat tgatattagt gcgttatatt
     2641 ttttgacaag ttaataaacg tgatccaccg cacgctttgt cgcccaccag gcggagcgaa
     2701 tgactaccct taaagaaaag cccgataatt agcgacgaat ttcggaggtt ggatccttat
     2761 gctcaatcag aaaattcaaa accctaatcc agacgaactg atgatcgagg tcgatctctg
     2821 ctatgaactg gacccgtatg aattaaaact ggatgagatg atcgaggcag aaccggaacc
     2881 cgagatgatt gaagggctgc ccgcctctga tgcgctgacg cctgccgatc gctatctcga
     2941 actgttcgag catgttcagt cggcgaaaat tttccccgac agtaaaacct ttcccgactg
     3001 cgcacccaaa atggacccgc tggatatttt aatccgctac cgtaaagtgc gccgtcatcg
     3061 tgattttgac ttgcgcaagt ttgttgaaaa tcacttctgg ctgccggagg tctactccag
     3121 cgagtatgta tcggacccgc aaaattccct gaaagagcat atcgaccagc tgtggccggt
     3181 gctaacccgc gaaccacagg atcacattcc gtggtcttct ctgctggcgc taccgcagtc
     3241 atatattgtc ccgggcggcc gttttagcga aacctactat tgggattcct atttcaccat
     3301 gctgggactg gcggaaagtg gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc
     3361 ctggatgatc gaaaactacg gtcacatccc caacggcaac cgcacctatt atttgagccg
     3421 ctcgcaacca ccggtttttg cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg
     3481 tgcgcgccgc tatctcgacc accttaaaat ggaatatgcc ttctggatgg acggtgcaga
     3541 atcgttgatc cctaatcagg cctatcgcca tgttgtgcgg atgccggacg gatcgctgct
     3601 caaccgttac tgggacgatc gcgacacgcc gcgtgacgaa tcctggcttg aggacgttga
     3661 aaccgcgaaa cattctggtc gcccgcccaa cgaggtgtac cgcgatttac gcgcgggagc
     3721 ggcctccggt tgggattact cttcccgttg gctgcgtgat actggtcgtc tggcgagcat
     3781 tcgtaccacc cagttcatcc ccatcgatct gaatgccttc ctgtttaaac tggagagcgc
     3841 catcgccaac atctcggcgc tgaaaggcga gaaagagaca gaagcgctgt tccgccagaa
     3901 ggccagtgcc cgtcgcgatg cggtaaaccg ttacctctgg gatgatgaaa acggcatcta
     3961 ccgcgattac gactggcgac gcgaacaact ggcgctgttt tccgctgccg ccattgtgcc
     4021 gctctatgtc ggcatggcga accatgaaca ggccgatcgt ctggcaaacg ccgtacgcag
     4081 ccggttactg acacctggcg ggattttggc aagcgagtac gaaaccggtg aacagtggga
     4141 taaacccaac ggctgggcac cgttacaatg gatggcaatt cagggattta aaatgtatgg
     4201 cgatgacctt ctgggtgatg aaatcgcgcg cagctggctg aaaacggtga atcagttcta
     4261 tctggaacag cacaaaatga tcgagaaata ccatattgcc gatggtgttc cccgcgaagg
     4321 cggcggtggc gagtatccgt tgcaggatgg gtttggctgg actaacggtg tggtacgccg
     4381 tttaattggt ttgtacggcg aaccataata tttttacagc cagccgctaa cttcctgctg
     4441 gctgtaaaat tatcctcttc
//
LOCUS       DADLZM010000213         4438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.217, whole genome shotgun sequence.
ACCESSION   DADLZM010000213 DADLZM010000000
VERSION     DADLZM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.217"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..212)
                     /locus_tag="JNY73_003901"
     CDS             complement(<1..212)
                     /locus_tag="JNY73_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY8049716.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPV"
     gene            522..2294
                     /gene="aspS"
                     /locus_tag="JNY73_003902"
     CDS             522..2294
                     /gene="aspS"
                     /locus_tag="JNY73_003902"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY8049717.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLGGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2412..2864
                     /gene="nudB"
                     /locus_tag="JNY73_003903"
     CDS             2412..2864
                     /gene="nudB"
                     /locus_tag="JNY73_003903"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY8049718.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2893..3633
                     /locus_tag="JNY73_003904"
     CDS             2893..3633
                     /locus_tag="JNY73_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8049719.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3668..4189
                     /gene="ruvC"
                     /locus_tag="JNY73_003905"
     CDS             3668..4189
                     /gene="ruvC"
                     /locus_tag="JNY73_003905"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY8049720.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4191..>4438)
                     /locus_tag="JNY73_003906"
     CDS             complement(4191..>4438)
                     /locus_tag="JNY73_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049721.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1098 a         1105 c         1233 g         1002 t
ORIGIN      
        1 tcaaccggct gttttaatgc ttcggcgtaa tcggcagacc agccaactcg caccagaaac
       61 acgggctgac cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca
      121 tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc
      181 aacgcagtgg ttttagcatt aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc
      241 ctcacacctt aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt
      301 tcgggcaaca cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg
      361 gtaacgaggc gttccagcgc gggccaggct cttacggtgc ggcagaattt ccagtataat
      421 agccgccttt tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc
      481 agcagggggc atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg
      541 gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc
      601 gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg
      661 tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt
      721 tctgcattca ggttacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata
      781 tggcgaccgg cgaaattgaa gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg
      841 ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc
      901 tcgacctgcg tcgcccggaa atggcgcagc gcctgaaaac ccgcgctaaa atcaccagcc
      961 tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg atgctgacca
     1021 aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat
     1081 tctacgcact gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggttttg
     1141 accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc
     1201 ctgaatttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag
     1261 tgatggaagc gctggtgcgt catttgtggc tggaagtgaa gggcgtggat ctgggcgatt
     1321 tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa ccggatctgc
     1381 gtaacccgat ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat
     1441 ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg
     1501 catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga
     1561 aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctgggaggt atcaacagcc
     1621 cggtagcgaa gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc
     1681 aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgggatgg
     1741 gcgcactgct cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac
     1801 cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa
     1861 tgcaccatcc gttcacctca ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg
     1921 aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt
     1981 cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg
     2041 aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc
     2101 cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgact ggcaccgaca
     2161 atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag
     2221 caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg
     2281 ctgagaataa ctgatatgac tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa
     2341 ctgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa ggcagtcaac
     2401 gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct
     2461 atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc gatttctggc
     2521 agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag
     2581 taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gactgtcagc
     2641 gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc
     2701 gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg
     2761 aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga
     2821 gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct
     2881 ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg
     2941 caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct
     3001 aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca
     3061 ctgtctaaca acatgacccg tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt
     3121 gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca
     3181 atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcacgca
     3241 ttcagcaaat gtggcggtaa cctcggcact gacggttccg tagcttatct gttcagcaaa
     3301 aaaggcgtga tctccttcga gaaaggcgac gaagacacca tcatggaagc agctctggaa
     3361 gcaggtgctg aagacgttgt gacatatgat gacggcgcga ttgatgtcta cactgcatgg
     3421 gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg
     3481 gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc gccgaaactg
     3541 atgcgtctga tcgatatgct ggaagattgc gatgacgtgc aggaagttta ccataacggt
     3601 gaaatctctg atgaggttgc agcgactctc tgatgatgcc tgctaaacag caaaacggag
     3661 acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg gctacggcgt
     3721 catccgccag gtaggtaggc aactgtctta cctgggtagc ggatgcatac gcaccaaagt
     3781 ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca
     3841 gttccagcct gattatttcg ccattgaaca agtctttatg gcgaagaacg ctgactcagc
     3901 cctgaaactg ggccaggcgc gcggcgtggc gattgttgcg gcggtgaatc aggaattgcc
     3961 ggtatttgaa tacgcggcac gtcaggtaaa gcaaacggtg gtaggtatgg gtagtgccga
     4021 aaaaagccag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc
     4081 ggatgccgcc gatgcgctgg cgattgccat cacccactgc cacgttagtc agaatgcgat
     4141 gcagatgagc gaatcgcggc tgaacctggc gagagggcga ctgcgttaag ttataccgcc
     4201 tcggtcagtt ctggctgagg cgtttcaact ccctccgcgt gaatcaacac caaacccgga
     4261 tgatgccaca aactggctgg cgtgacggtt ttacgctccc acggaataga acctaagaac
     4321 cagaatttcc agaaagtgag ttttgcattc ggattgctat ttaacagttg tccaaacgtc
     4381 tctatttcac ccaccggaat acatagcgcc tctttataaa tatcaaagac aaattttg
//
LOCUS       DADLZM010000214         4378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLZM010000214 DADLZM010000000
VERSION     DADLZM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.218"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(64..822)
                     /locus_tag="JNY73_003907"
     CDS             complement(64..822)
                     /locus_tag="JNY73_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY8049722.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQYFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JNY73_003908"
     CDS             complement(830..1933)
                     /locus_tag="JNY73_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY8049723.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="JNY73_003909"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="JNY73_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="JNY73_003910"
     CDS             complement(3181..4206)
                     /locus_tag="JNY73_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8049724.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1215 a         1146 c         1022 g          995 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatactg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DADLZM010000215         4342 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLZM010000215 DADLZM010000000
VERSION     DADLZM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.219"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..368)
                     /locus_tag="JNY73_003911"
     CDS             complement(<1..368)
                     /locus_tag="JNY73_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8049725.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(483..558)
                     /locus_tag="JNY73_003912"
     tRNA            complement(483..558)
                     /locus_tag="JNY73_003912"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Thr,seq:ggt)
     gene            complement(565..639)
                     /locus_tag="JNY73_003913"
     tRNA            complement(565..639)
                     /locus_tag="JNY73_003913"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Gly,seq:tcc)
     gene            complement(756..840)
                     /locus_tag="JNY73_003914"
     tRNA            complement(756..840)
                     /locus_tag="JNY73_003914"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Tyr,seq:gta)
     gene            complement(849..924)
                     /locus_tag="JNY73_003915"
     tRNA            complement(849..924)
                     /locus_tag="JNY73_003915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(889..891),aa:Thr,seq:tgt)
     gene            1286..2236
                     /gene="coaA"
                     /locus_tag="JNY73_003916"
                     /gene_synonym="panK"
     CDS             1286..2236
                     /gene="coaA"
                     /locus_tag="JNY73_003916"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY8049726.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2265..3230)
                     /gene="birA"
                     /locus_tag="JNY73_003917"
     CDS             complement(2265..3230)
                     /gene="birA"
                     /locus_tag="JNY73_003917"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY8049727.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3227..4255)
                     /gene="murB"
                     /locus_tag="JNY73_003918"
     CDS             complement(3227..4255)
                     /gene="murB"
                     /locus_tag="JNY73_003918"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY8049728.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1109 a         1085 c          982 g         1166 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcgcgagca
      241 gcaccgccgt aggttttagc cagtacggta gtgattgcag cggtcagcgt tgttttacca
      301 tggtcaacgt ggccgatagt accgacgtta acgtgcggtt ttgtacgttc aaacttttct
      361 ttagacatcg attgtccctc taagacacgg ataaatcggt gatatcacca catcaaccag
      421 gcaacatgcc cgacttgttg aatgcaataa acagaaggaa aaaacaggga ggagaaaaga
      481 agtggtgctg ataggcagat tcgaactgcc gacctcaccc ttaccaaggg tacgctctac
      541 caactgagct atatcagcac atcttggagc gggcagcggg aatcgaaccc gcatcatcag
      601 cttggaaggc tgaggtaata gccattatac gatgcccgca tcctagaact cggctacctg
      661 attttcattc tgcactgaat atcgagagaa gctctcttta ttcgagccgg taagtgaact
      721 tatcgtctcg ggctacgcca tcgcgtggcc gaaattggtg gtgggggaag gattcgaacc
      781 ttcgaagtct gtgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc
      841 ggacttgatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
      901 ctctacctac tgagctaagt cggcatcaag tagcgcgcat tctatggaga catgcgagtt
      961 catgcaacta aaaaattgca caatttgttt tattggtcac attttatgct acacgatgaa
     1021 gaaacagccg aacggagggc agattagcac actttttcaa catcattgtg ctcaacaatg
     1081 cgctcctgct aaaccataat tctttttatc agatggaata tctgtcacat tgcttttcaa
     1141 cgatagcttc ctggcagaga ttttttctta ttattcctcc ccatctggtg ttaccctcct
     1201 gcccattaac ccattcaaca gaactgtgac gcgccatggc aaatatcgct ttgccgatag
     1261 agctatgacc gccagaaaca tgcttatgag tataaaagag caaacgttaa tgacgcctta
     1321 cctacagttt gaccgcaacc agtgggcagc tctgcgtgat tccgtaccta tgacgttatc
     1381 ggaagatgag atcgcccgtc tcaaaggtat taatgaagat ctctcgttag aagaagttgc
     1441 cgagatctat ttacctttgt cacgtttgct gaacttctat ataagctcga atctgcgccg
     1501 tcaggcagtt ctggaacagt ttcttggtac caacgggcaa cgcattcctt acattatcag
     1561 tattgctggc agtgtcgcgg tggggaaaag tacaaccgcc cgtgtattgc aggcgctatt
     1621 aagccgttgg ccggaacatc gtcgtgttga actgatcact acagatggct tccttcaccc
     1681 taatcaggtt ctgaaagaac gtggtctgat gaagaagaaa ggcttcccgg aatcgtatga
     1741 tatgcatcgc ctggtgaagt ttgtttccga tctcaaatcc ggcgtgccaa acgttacagc
     1801 ccctgtttac tcgcatctta tttatgatgt gatcccggat ggagataaaa ctgttgttca
     1861 gcctgatatt ttaattcttg aagggttaaa tgtcttacag agcgggatgg attatccaca
     1921 cgatccacat catgtatttg tttccgattt tgtcgatttt tcgatatatg ttgatgcacc
     1981 ggaagactta cttcagacgt ggtatatcaa ccgttttctg aaattccgcg aaggggcttt
     2041 tacagacccg gattcctatt ttcataacta cgcgaaatta actaaagaag aagcgattaa
     2101 gactgccatg acattgtgga aagagatcaa ctggctgaac ttaaagcaaa atattctacc
     2161 tactcgtgag cgcgccagtt taatcctgac gaaaagtgct aatcatgtgg tagaagaggt
     2221 cagactacgc aaataatttg caggggagcg aatactcccc tttcttattt ttctgcacta
     2281 cgcagggata tttcaccgcc catccagggt tttattattc catcctgctc aagtaataaa
     2341 gccccctgtt tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc aatgataagt
     2401 ttcactgggc gattaataaa attatccagc ttttcccagc gcgacagata aggtgccaat
     2461 ccttcttgtt cgaagagttc caacgcagca cgtaattcac gtattagcat ggccgccaac
     2521 gtattacgat caagattgat ccccgcttcc tgcagcgtga tccacccctg attaacgaca
     2581 ctctcttcaa cacggcgcat tgccatgttg atcccggatc caatgactat ttgcgccgca
     2641 tcgccagttt tgccagtcag ctccacaaga atgcctgcca gcttgcgatc ctgcagatag
     2701 aggtcattag gccatttgac acgaacttta tctgctccca gctttcgtaa tacttccgcc
     2761 atcacgatac cgataaccag acttaaacca atcgccgccg ccgggccttg ttccagacgc
     2821 cagaacatcg acaaatataa gtttgcgcca aaaggcgaaa accatttccg cccccggcga
     2881 ccacggccag cctgctggta ttctgcaaca caggcatcgc ccgatttaag ctctccgata
     2941 cgatcaagaa ggaactgatt cgtggagtca ataactggca gcacggctac actaccgcca
     3001 tccagctgac ccaatatctg ttcagcatta agtaactgga tgggctcagg caggctgtat
     3061 cctttacccg gaacggtaaa gacatcaacg ccccagtcac gcagtgtctg aatgtgttta
     3121 ttaatagccg cccggctcat tcccagcgtt tcacccaact gctcgccaga gtgaaattca
     3181 ccgttcgcta acagggcaat caatttcagt ggcacggtgt tatccttcat gaaattgtct
     3241 ccactgcgct cacttcacct gatgcaccaa taaagcggac ttcaggctca agccagacat
     3301 taaatttttc acccactttt tgtcttacat ggtgtgccag ttgcaccaca tcttcgcttt
     3361 ttgcattgtc ttcattaatg agaactaacg cctgttgacg gtgcaccgca gccccaccca
     3421 tttgcatccc ttttagctgg cactgatcaa taagccaacc tgctgccagt tttactgaac
     3481 cacccgcctg ggggtaattt ggtgctgttg gaaattgcgc cagtaatgct ttagccgttt
     3541 cggcagatac aacagggttt ttgaagaaac taccggcatt gccattcact tttggatcag
     3601 ggagtttggt ggtgcgcata tgacacaccg cattaaatac ttgctgtggc gttactgttg
     3661 taggatccag acgagttaag tcaccatacg ttagtacagg ttgccactct tttggcagac
     3721 gcagacctac ggctacaatg gcgaagcgat cctggtattc atgtttaaaa atactgtcgc
     3781 gatagccaaa acggcactct ttggcagtta agcgcacttg cttgcctgtc gccagttcaa
     3841 cacaatcaac ataagcgcaa actcgctgta attctacgcc ataagcacca atattctgga
     3901 taggtgatga gccgacacaa ccaggaatta atgccagatt ttccagacca ggcatacctt
     3961 cctgcaaagt gtattttacc aggcgatgcc agttttctcc ggctcctaca tgtaaatacc
     4021 acgcatcagg ttcatcatga atttcgatac ctttgatccg gttgatgatc accgtgccgc
     4081 gatagtcttc cagaaaaagt acattacttc cttcacccag aataagaacg ggttgtcctt
     4141 ctgcggttgc atgctgccag gcattgagta attgttgttc gtcttcggca catacaatgt
     4201 gctgagcatt atgatcaatg ccaaatgtgt tccagggttt taaggagtgg ttcatagctg
     4261 ctttcctgat gcaaaaacga ggctagttta ccgtatctgt ggggggatgg cttgtagata
     4321 tgacgacagg aagagtttgt ag
//
LOCUS       DADLZM010000216         4336 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLZM010000216 DADLZM010000000
VERSION     DADLZM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.220"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..171)
                     /locus_tag="JNY73_003919"
     CDS             complement(<1..171)
                     /locus_tag="JNY73_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8049729.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTEVY"
     gene            complement(321..482)
                     /locus_tag="JNY73_003920"
     CDS             complement(321..482)
                     /locus_tag="JNY73_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049730.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(479..682)
                     /locus_tag="JNY73_003921"
     CDS             complement(479..682)
                     /locus_tag="JNY73_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY8049731.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            927..1262
                     /gene="iraM"
                     /locus_tag="JNY73_003922"
     CDS             927..1262
                     /gene="iraM"
                     /locus_tag="JNY73_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY8049732.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1543..1674)
                     /locus_tag="JNY73_003923"
     CDS             complement(1543..1674)
                     /locus_tag="JNY73_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY8049733.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2174..2250)
                     /locus_tag="JNY73_003924"
     tRNA            complement(2174..2250)
                     /locus_tag="JNY73_003924"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Arg,seq:tct)
     gene            complement(2352..2427)
                     /locus_tag="JNY73_003925"
     tRNA            complement(2352..2427)
                     /locus_tag="JNY73_003925"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ile,seq:cat)
     gene            complement(2571..3392)
                     /locus_tag="JNY73_003926"
     CDS             complement(2571..3392)
                     /locus_tag="JNY73_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY8049734.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3407..3763)
                     /locus_tag="JNY73_003927"
     CDS             complement(3407..3763)
                     /locus_tag="JNY73_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8049735.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3776..>4336)
                     /locus_tag="JNY73_003928"
     CDS             complement(3776..>4336)
                     /locus_tag="JNY73_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY8049736.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1188 a         1181 c          858 g         1109 t
ORIGIN      
        1 gtacacctca gtcagatatg tcagcagccc aaacagcaga ctccccagca cgcctattgc
       61 cgcccactga gacggggaaa acctgtccag caactgcagg aaccagtagc ccgttcccac
      121 cgctgacgtg gtgtatgaca cacctgttgt gattttttcc atctggtcca taccccgtct
      181 cccgttatcc ggaagctgac aacaataaaa aagccaccag ttaactactg atggctctga
      241 taactcatgc aagcgtctca gacgacccac tgacactacc ggtgagttta acgatacctt
      301 ccatttgact ggctcacttt ttatgatgat gccggtgcat ttatctccag caccagactt
      361 tctatctcaa cgccatacgt tgcatttttg gtaatatccg tcagcgtcag tgcatttagt
      421 cccactgcca gactgtcttt tatggcctgg aatgccgggc cagtacgatg acgtagtatc
      481 actccggctc agttgcacca ctgaccacca catcaccttc tgctgcaatc gcctgcatca
      541 gggtataagg ggttatggcc accggactac caaacggctg ccagccctct ttcagtttat
      601 gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca tagtgtttaa
      661 atgccatggt tctttccacc atctgaaaaa taattcttta aaatacctga catgtaatac
      721 agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga tatgcatgga
      781 taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa caacatcaga
      841 aaaataattc tgctctatgg tttaattcaa aaatatcatt tatacttttc agaacatcac
      901 cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca gttatccagc
      961 catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc
     1021 aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc
     1081 tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct
     1141 ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta ttgaatgaat
     1201 gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat
     1261 aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga
     1321 gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca
     1381 cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt
     1441 caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa
     1501 ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt
     1561 ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccgg
     1621 cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta
     1681 ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc
     1741 ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta
     1801 gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct
     1861 cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct
     1921 cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca
     1981 atattcactc tacccacacc atatgaatga taaatactta atgctgaaaa aaccattaga
     2041 ccgtataaca aatactcaat caatacttaa cagaactttt atttttgaca aatatataat
     2101 attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct
     2161 ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc
     2221 aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga
     2281 cccgcccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga taagctaatg
     2341 gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     2401 tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg taattaattt
     2461 cgcaataaaa aacccgctca gcggcgggtt gtagaaactc ttctaacgtc aggcataaaa
     2521 cgcccatcat tatgacgaat ttaccacaga ttccggaaaa atcaaccttg ttacctagtt
     2581 acctttttta actgccgctc agcccatgct tcttcaatat caaaccgggt caccagcgca
     2641 tcataaaatt tcttaactgt tttttcccat gacgcgcgtg ttatctggtt tgtcacctcg
     2701 catatagcat taaatgcctc cgttgatggt agtctttcat agccacgacc accacaacgc
     2761 tggcagtctc tgataacagg cataccacgt tttaccgact cttcacgatg aatggcgaca
     2821 ccacgcccac ggcaatcctt acaggcggtg gaaacctcac cctttccgcc acactccgga
     2881 caggcaactt ttaccacctc cctgactttt ttccattctt cccagtaaga cggatacaca
     2941 cctttcgtgc actttgccca taccggcggc ttaccatccg gatactggat cttgtttgta
     3001 aaaactatgc tttcaataaa tttttcccca tagcaacaag ggcactgctt tttactcgct
     3061 gcgctgcggg cataatcctc aaaagcgtac gaagccataa tgcgcatcac taccggtttt
     3121 atttctgccg gaagttttct caacgccgcc acacgatcgc accgactgag tgcataatct
     3181 gccagtaatt ctgttgcccg cgccctgtca ttcatactga tgcccatttt ccccaggaac
     3241 gcagaaaaac ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg
     3301 atactcagcg catcttttga cgttgaggcc gatgcatcgg tcaggccagg ggattttggg
     3361 gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgctcttaa tgcattatgg
     3421 ggtaaatctt cacccccaga cgtccaccag atactggctg accacgaacg atattgattt
     3481 catcaaactg ctcatcgtcc attaacactc ccgcatgcgt cagcgcatcc agcggtgctt
     3541 tcaggatatt gtccaggtcg cgacgacgct tatccggtgg ctctgcaatc acctttatcg
     3601 ccagccttcc ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc
     3661 ggcgataacg ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag
     3721 tgtttaccgt cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc
     3781 caagcacgcc agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac
     3841 catgcttttc ttcgaacgcc agaggatccg catgaagctc gttgtgatgc tcccgacaca
     3901 gcggtagcgt gaaaatatcg tgagattttg tccccattcc gccctgacca tgaccaatca
     3961 ggtgatgggg atcgtcggct ggcttaccac aacacgcaca cggctgtgtc ttcacccagc
     4021 gtttgtattt ctcgttaacc cagctgcgac gtttaggtcg tttcatgaaa gattccggag
     4081 actcaggatc aacggcaatg ctgaccaccg tcttttcctg ttgtgggttc tgttgctggt
     4141 gggcgtgagg cagcggcgca agattttttg tgcgctgttt cagtatgctg gtggcggtct
     4201 gctctcccgg tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc
     4261 ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc
     4321 accaggataa ttcagc
//
LOCUS       DADLZM010000217         4327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLZM010000217 DADLZM010000000
VERSION     DADLZM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.221"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..324
                     /locus_tag="JNY73_003929"
     CDS             <1..324
                     /locus_tag="JNY73_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HAY8049737.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="JNY73_003930"
     CDS             383..586
                     /locus_tag="JNY73_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY8049738.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRELFTCNKTLPPHEVEPNFGAI"
     gene            586..1623
                     /locus_tag="JNY73_003931"
     CDS             586..1623
                     /locus_tag="JNY73_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8049739.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPSNSKCDFCH"
     gene            complement(1651..1740)
                     /locus_tag="JNY73_003932"
     tRNA            complement(1651..1740)
                     /locus_tag="JNY73_003932"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1704..1706),aa:Ser,seq:cga)
     gene            1834..2631
                     /gene="mtfA"
                     /locus_tag="JNY73_003933"
     CDS             1834..2631
                     /gene="mtfA"
                     /locus_tag="JNY73_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY8049740.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2732..2807
                     /locus_tag="JNY73_003934"
     tRNA            2732..2807
                     /locus_tag="JNY73_003934"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2765..2767,aa:Asn,seq:gtt)
     gene            2969..4231
                     /locus_tag="JNY73_003935"
     CDS             2969..4231
                     /locus_tag="JNY73_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8049741.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGSELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1207 a          996 c         1036 g         1088 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gagctattta cctgcaataa
      541 aactttacca ccacacgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagtaatt ccaagtgtga tttttgtcac
     1621 tgacttaatg atgtataagt gattgaattt tggcggagag agggggattt gaacccccgg
     1681 tggaggtgcc cccactccgg ttttcgagac cggtccgttc agccgctccg gcatctctcc
     1741 gctcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct gtccgtgcaa
     1801 ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg gaaagtacaa
     1861 gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc ccttttaacg
     1921 tgtctgacag aacaggaaca aagcaaattg gtcgctcttg ccgaacgttt tttacagcaa
     1981 aaacggcttg ttcctttaca gggctttgag ctgaattcat taagaagctg ccggatagca
     2041 cttctatttt gcctgcccgt tctggagtta ggactggaat ggctggatgg ttttcatgaa
     2101 gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga tatcggtctg
     2161 gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc tatcgttttg
     2221 aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat tattcatgaa
     2281 gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc ctttatttcg
     2341 ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa cattcaggaa
     2401 gaaatcgaat tagttggtga gaatgcggcg agcattgatg cttatgctgc cagtgatcct
     2461 gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact ttttgctcct
     2521 cgtttccctt cattgtggca acgtttctgt caattttatc aacaagatcc tttgcagaga
     2581 ctgcatcacg ctaatgatac agactcgttt tcggcgacga atgttcatta attaacaact
     2641 ttgcagatta attaaccaat tgaaatggct tatgaaattt agtgttgaca gacaaggtac
     2701 cgctaagtaa tatgcgcccc gttctcacga ttcctctgta gttcagtcgg tagaacggcg
     2761 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaatt ttctgttttc
     2821 atgcatcctt gcgaatcttt atctgatgtt gattcaacag gttagtgtga aaactcttct
     2881 cggtgcattt tgattttacc ctacgcatcg ggaaaaattg gtggtcaaat ctgggatcag
     2941 gttagttcga taatggagtg acccccatat atcccttacc gacgcaaaaa tccgcaccct
     3001 caagccttct gataaaccct ttaaagtctc cgattctcac ggtctgtatc tgctggtcaa
     3061 gccgggtggc tcccgccact ggtatctcaa ataccgtatt agcggtaaag aatcccgcat
     3121 tgcgctgggt gcctatccag ccatctccct gtctgatgcg cgacagcaac gtgaaggtat
     3181 ccgtaaaatg ctggcgctga atatcaaccc ggtacagcag cgggctgctg aacgtggctc
     3241 acgaacaccg gagaaagttt ttaaaaacgt ggcgctggcg tggcataaaa gtaacaggaa
     3301 atggtcgcag aacaccgccg accgtctgct tgccagcctg aacaatcaca tctttccggt
     3361 catcgggaac ctacctgtat cagaacttaa accccgtcat ttcattgacc tgctgaaagg
     3421 gatcgaggaa aaaggtctgc tggaggttgc gtcccgcaca cggcagcacc tgagtaacat
     3481 aatgcgccat gcggtccatc tggagttaat cgatacgaac cctgcagcaa accttggcgg
     3541 cgtgaccaca cctcctgtca gacggcacta tcctgccctg ccgctggagc ggctgcctga
     3601 actgcttgaa cgtattgggg catatcatca gggcagtgaa ctgacccggc atgccgttct
     3661 gctgatgctg catgtgttca ttcgctccag tgaactgcgt ttcgcccgct ggtcagagat
     3721 tgatttcaca aaccgagtct ggacgatacc cgcgacgcga gaacccatta ttggcgtgcg
     3781 ttattccggc cgcggggcaa aaatgcgaat gccgcatatc gtccccctct cagaacagtc
     3841 catcgccatt ctgaaacaga ttaaggatat caccggtaat aatgaactga tcttccccgg
     3901 cgaccataac ccgtataagc caatgtgtga aaacacggtc aataaggcac tgcgggtgat
     3961 gggttacgac acgaaaaagg atatctgcgg tcacggcttc cgggcaatgg catgcagtgc
     4021 gctgatggaa tcgggtttat gggcaaagga cgcagtagaa cgccagatga gtcatcagga
     4081 gcgcaatacc gtgcgcatgg cttatattca taaggcagag cacctggaag cccgcaaagc
     4141 gatgatgcag tggtggtcgg attatctgga agcatgccga gaatcttatg caccgcctta
     4201 tacaattggt aaaaataagt ttatcccata gtatactaac gaatcccaca ctctggcaaa
     4261 ttaccaccac ctctacagga ggtggttcta tatagacttt tatcaacttt atgacgaaaa
     4321 acaccta
//
LOCUS       DADLZM010000218         4326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLZM010000218 DADLZM010000000
VERSION     DADLZM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.222"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..157)
                     /locus_tag="JNY73_003936"
     CDS             complement(<1..157)
                     /locus_tag="JNY73_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049742.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNY73_003937"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNY73_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY8049743.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="JNY73_003938"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="JNY73_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY8049744.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATKTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNY73_003939"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNY73_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY8049745.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..>4326)
                     /locus_tag="JNY73_003940"
     CDS             complement(3800..>4326)
                     /locus_tag="JNY73_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY8049746.1"
                     /translation="TTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
BASE COUNT         1143 a         1083 c          993 g         1107 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg tttttgtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacgacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatgcca cgaaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcgcttct gctctgccag
     4321 tggtgc
//
LOCUS       DADLZM010000219         4239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLZM010000219 DADLZM010000000
VERSION     DADLZM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.223"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(96..377)
                     /locus_tag="JNY73_003941"
     CDS             complement(96..377)
                     /locus_tag="JNY73_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY8049747.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(639..1748)
                     /gene="apbC"
                     /locus_tag="JNY73_003942"
     CDS             complement(639..1748)
                     /gene="apbC"
                     /locus_tag="JNY73_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY8049748.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1880..3913
                     /gene="metG"
                     /locus_tag="JNY73_003943"
     CDS             1880..3913
                     /gene="metG"
                     /locus_tag="JNY73_003943"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY8049749.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4054..>4239
                     /locus_tag="JNY73_003944"
     CDS             4054..>4239
                     /locus_tag="JNY73_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY8049750.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
BASE COUNT         1088 a         1065 c         1051 g         1035 t
ORIGIN      
        1 tatgtaaatg gtattgttga tatcaataaa aaagacgaaa tcatttatat aaatgatttc
       61 gtctttctaa atgcaattaa aatcaggttg ccaaattaat cgtagcgatt taacacgatt
      121 cgacgtttgc tatcgctacc tgcaacaacc acttctgtcg taacaccttt gacgggcgaa
      181 gaaacctgat gacgggtgtc aacattatac gttaactgac cacaatgctg ttgtggttga
      241 ctattaacca tagccatact gcatgttggg ggactgatcc tgccattaat cgttaatgtg
      301 gcagtctctg aagagactgc gggagaagcc aatccatagg caagaaaaat aattcctgac
      361 agccaatact tattcattga acgttatccc tgtagtaaag gttatgcctg gatagaatga
      421 gtgcataaca aactatagct gtacatccac tacacagcac gaaggatgat aatgaagcat
      481 tgcctgtacg atcactcgac tttgtagagt ttcggacgaa ggtccgcaga atattcgcag
      541 tattaaataa gtgttcatta atgcggcaaa aacaaataca ccgcatcaat ataacatctc
      601 tgcggtgtat gaagaataac cagaatggtt aataggcgtt agaccgcgcg gaaggaaatc
      661 tcgccaggaa tgacttcacc ctgccagtag agctgagctg caacgcggtc agccagctgg
      721 cggtagatag cggtaaattc gctctccgga cggctgataa ccgttggcgt gcctttatcg
      781 agatcctcac gtaaggagat gtgtaacggc atctgcccca gcagctgtgt atggtatttt
      841 tcagccagtt tctctgcacc accggtaccg aaaatcggtt catggtgacc acagttactg
      901 caaatatgca cactcatgtt ctcgacaata ccgagcaccg gaacctcgac tttctcgaac
      961 atcacaatgc cttttttcgc atcgatcaac gcgatgtcct gcggggtggt gaccacgact
     1021 gcaccagtta ctggaatgtt ttgcgccagc gtcaattgaa tgtcaccggt gcctggcggc
     1081 atatcaagca ccagatagtc gagatccggc cacaaggttt cctgcaacat ctgcattaac
     1141 gccttgctcg ccatcggtcc acgccacacc atcgcattgt cgtcagtgac cagataacca
     1201 attgaattgg ttgccaggcc atgagacata atcggtgcca tgtgggtacc gtccggcgaa
     1261 gttggacgct gattttctgc gcccagcatg gtaggaattg atgggccgta gatatcggcg
     1321 tccagaatgc cgactttcgc cccttccgcc gccagtgcca gcgccaggtt taccgccgtg
     1381 gacgatttac ccacgccgcc tttaccggaa ctcaccgcga taatgttctt cacaccgtta
     1441 attcccggct gattcttcac gcgtttcaga gtggctatgt tatgcgacag tttccagtcg
     1501 atagctttcg ccccggtgat gcgcagcagt tcggcgctac attgctcttt taactcttcg
     1561 aacgcgctat gccagacaaa cggcataacc agttccacat gcaacgtgtc gtccatccag
     1621 gcgacatggt gcaacgcttt cagcgtggtg aggttatgct tcagggttgg gtgctgaaaa
     1681 ttggccagtg tcccggcgac cattgcgcgc aaggcttctg gtgatttggc ctgggattgt
     1741 tcgttcatcc cgactccttt tttgtataga taaaccatca gctgatagtt tacctgaaga
     1801 atatagagaa gtacttactt aacattttcc catttggtac tatctaaccc cttttcacta
     1861 ttaagaagta atgcctacta tgactcaagt cgcgaagaaa attctggtga cgtgcgcact
     1921 gccgtacgct aacggctcaa tccacctcgg ccatatgctg gagcacatcc aggctgatgt
     1981 ctgggtccgt taccagcgaa tgcgcggcca cgaggtcaac ttcatctgcg ccgacgatgc
     2041 ccacggtaca ccgatcatgc tgaaagctca gcagctcggt atcaccccgg agcagatgat
     2101 tggcgaaatg agtcaggagc atcagactga tttcgcaggc tttaacatca gctatgacaa
     2161 ctatcactcg acgcacagcg aagagaaccg ccagttgtca gaacttatct actctcgcct
     2221 gaaagaaaac ggttttatta aaaaccgcac catctctcag ctgtacgatc cggaaaaagg
     2281 catgttcctg ccggaccgtt ttgtgaaagg cacctgcccg aaatgtaaat ccccggatca
     2341 atacggcgat aactgcgaag tctgcggcgc gacctacagc ccgactgaat tgatcgagcc
     2401 gaaatcggtg gtttctggtg ctacaccagt gatgcgtgat tctgaacact tcttctttga
     2461 tctgccctct ttcagcgaaa tgctgcaggc atggacccgc agcggtgcgt tgcaggagca
     2521 ggtggcaaac aaaatgcagg agtggtttga atccggcctg caacagtggg atatctcccg
     2581 cgacgcgcca tactttggtt ttgaaattcc gaacgcgccg ggcaaatatt tctacgtctg
     2641 gctggacgca ccgattggct acatgggttc tttcaagaat ctgtgcgaca agcgcggcga
     2701 cagcgtaagc ttcgataaat actggaagaa agactccacc gccgagctgt accacttcat
     2761 cggtaaagat atcgtttact tccacagcct gttctggcct gccatgctgg aaggcagcaa
     2821 cttccgcaag ccgaccaacc tgtttgttca tggctatgtg acggtgaacg gcgcgaagat
     2881 gtccaagtct cgcggcacct ttattaaagc cagcacctgg ctgaatcatt ttgacgctga
     2941 cagcctgcgt tactactaca ctgcgaaact ctcttcgcgc attgatgata tcgatctcaa
     3001 cctggaagat ttcgttcagc gtgtgaatgc cgatatcgtg aacaaagtgg ttaacctggc
     3061 ctcccgtaat gcgggcttta tcaacaagcg ttttgacggt gtgctggcaa gcgagctggc
     3121 tgaccctgag ctgtacaaaa cctttaccga tgccgctgaa gtgattggtg aagcgtggga
     3181 aagccgtgaa tttggtaaag cagtgcgcga aatcatggca ctggctgacc tggctaaccg
     3241 ctatgtcgac gagcaggctc cgtgggtagt cgcaaaacag gaaggtcgcg atgctgacct
     3301 gcaggcgatt tgctcaatgg gcatcaacct gttccgcgtg ctgatgactt acctgaagcc
     3361 ggtactgccg aaactgaccg agcgtgcaga agccttcctc aatacggaac tgacctggga
     3421 tggtatccag caaccgctgc tgggccacaa agtgaatccg ttcaaggcgc tgtataaccg
     3481 catcgatatg aaacaggttg aagctctggt ggaagcctct aaagaagaag tgaaagccac
     3541 taccgcgcca gttactggcc cactggcaga tgatccgatt caggaaacca tcacctttga
     3601 cgacttcgcc aaagttgacc tgcgcgtggc gctgatcgaa aacgcagagt ttgttgaagg
     3661 ttctgacaaa ctgctgcgcc tgacgctgga tctcggcggt gaaaaacgca atgtcttctc
     3721 cggtattcgt tctgcttacc cggatccgca ggcactgatt ggtcgtcaca ccattatggt
     3781 ggctaacctg gcaccacgta aaatgcgctt cggtatctct gaaggcatgg tgatggctgc
     3841 cggtcctggc gggaaagata ttttcctgct aagcccggat gccggtgcta aaccgggtca
     3901 tcaggtgaaa taatccccct tcaaggcgct gcatcgacag cgccttttct ttataaattc
     3961 ctaaagttgt tttcttgcga ttttgtctct ctctaacccg tataaatact ggtagcatct
     4021 gcattcaact ggataaaatt acaggaatgc agaatgagac actttatcta tcaggacgaa
     4081 aaatcacata aattctggac ggtggagcaa cagggaaacg agttgcatat cagttgggga
     4141 aaagttggca ccaaagggca aagtcagata aaaagttttt cagatgctgc ggcggcggaa
     4201 aaagcggaac tgaagctgat tgcggagaag gtgaagaag
//
LOCUS       DADLZM010000220         4223 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLZM010000220 DADLZM010000000
VERSION     DADLZM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.225"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            154..768
                     /locus_tag="JNY73_003945"
     CDS             154..768
                     /locus_tag="JNY73_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY8049751.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            768..1097
                     /locus_tag="JNY73_003946"
     CDS             768..1097
                     /locus_tag="JNY73_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY8049752.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            1109..1999
                     /gene="cyoE"
                     /locus_tag="JNY73_003947"
     CDS             1109..1999
                     /gene="cyoE"
                     /locus_tag="JNY73_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY8049753.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FSANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            2184..2495
                     /locus_tag="JNY73_003948"
     CDS             2184..2495
                     /locus_tag="JNY73_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8049754.1"
                     /translation="MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            2479..3021
                     /locus_tag="JNY73_003949"
     CDS             2479..3021
                     /locus_tag="JNY73_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8049755.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPATINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(3077..4012)
                     /locus_tag="JNY73_003950"
     CDS             complement(3077..4012)
                     /locus_tag="JNY73_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8049756.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT         1025 a         1003 c         1015 g         1180 t
ORIGIN      
        1 gggcgcttac ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
       61 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
      121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
      181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt
      241 tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac ctatgccgtt
      301 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
      361 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
      421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
      481 gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt
      541 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
      601 gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
      661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
      721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
      781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
      841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg
      901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
      961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     1021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     1081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     1141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     1201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     1261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     1321 gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     1381 cttgctgggt attgctggct ttatgctgct gtggtttagc gcgaatccgc tggcctgctg
     1441 gctgggggtg atgggctttg tggtttatgt cggcgtctat agcctgtaca tgaaacgcca
     1501 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg tgattggcta
     1561 ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     1621 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt accaggcggc
     1681 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     1741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     1801 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ctctgcgcgg
     1861 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     1921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact cgcatacgct
     1981 gctggctgct gtgtggtaac aaaacctctc tattaaaaag gtgctacggc acctttttta
     2041 ttgtctgaga tcagagatac ggctaataac cgtatctctt gacagagtga gcagatgcac
     2101 ataaattaat catacggttg atagccgtat tatgtatgag atcgactccc tacgatttca
     2161 ttcagttaat caaagaagtc ataatgcttg gaaaaacagc cgctctatac gatgtcgata
     2221 aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa aacaaacttc
     2281 gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc gctgctatgg
     2341 aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat caaagctggg
     2401 aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc aaggcgttaa
     2461 tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc catagtcctc
     2521 tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga ttgcggtaac
     2581 cctgttatcg ataaatttgt acgcgcatcg ctaaagaaaa gtgtgcgtaa tagcgactgt
     2641 gcggctaaag cacttattga cagacaaagt ggtgaactga ttggcatctg tacttttacg
     2701 gcatattcgc tggaaagaca acgcgtttct ggcgtccttc agggttcaca accttcagaa
     2761 attggtgttg tcagattagt catgttgggg ggagcgcgga agtatcaaaa gcggggcttt
     2821 ggtcaggacc tactatgtga tttttttgaa catgtaaaaa aaattcacca ggcattacca
     2881 attaaagggg tttatcttga tgctgaccct gccaccatta acttttatgc tcgtctcggc
     2941 tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt tttggcgatt
     3001 cagcatattc tcgcggctta gattattatc gccacccgcc agggcaagtt ttgccttagc
     3061 cgggggctga gcagcgttac cacttttgtt caattgcccc cgctttcagc aactccacat
     3121 ccttgccgtt aatagttgcc gttgcggcaa cgattgtggt ccttacagtt gcacaagatt
     3181 ttcgctcaaa ttccggttta taaagaataa gagacgaatt actggtaaat gaaccaaata
     3241 ggtaatcagt aaagctaata ttcagccggt ttaaaccgaa aggcgcgaag ccaaccgttt
     3301 ggttaatctc cttaccggat gggcgacgaa tgctgaatgt cacgcgaaga ttggccactt
     3361 cgcctaattt attggtggtg gcgaagaggc tatccatgca ggaaaaacct ttatctaaaa
     3421 aattatcccg catcccagaa cgggtaaccg tgatcacggg taacgtatcc tcaaccgttt
     3481 ttctgtcaat catgtctttt cgacaagtag ccagaaagtc ttccgggcca ccataaatgt
     3541 ggacaccttt atcaaggtat tccttcgctt tcttacaatc tacctcaaca tactgaccat
     3601 tggcgtacat catgcccaac ctggaccatg ctaaattcat accttgctgc gcggcctgat
     3661 taaatagtgc atacgctttg gcataatcca cccgcacctg ctcaccgtcg tagtagatac
     3721 tccccagcgc taacggagct tctctgtcac cttgtttagc ggctaactcg aaccatttaa
     3781 tcgcttcctg aacatcaggt ttggtatcat agcgaggctg ataatattcc cagcctaaca
     3841 ggctctgcgc gtcgatatct cccgattcag ccattttatg cagtgtattt ataacatcgg
     3901 gagcagattt accaccgtat atgctgtcat acaattcatg cgctttctta cttgccacca
     3961 cgcctgatga aactatcagc attaaagaaa ataatattct attaggcaac ataaaatatc
     4021 tcttatttat cttgtacctg aatatgctct tcttttaata aatcacgttg ttttccattc
     4081 agtatcgctg tcgcagattt aatagaataa gtcaaatcat gaaaatcgca taaattacga
     4141 ttatacattg ggatgtctat atagcttttt cttatataac tggaattatt atacatatcc
     4201 acaccgagcg tattcaaact aaa
//
LOCUS       DADLZM010000221         4213 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.226, whole genome shotgun sequence.
ACCESSION   DADLZM010000221 DADLZM010000000
VERSION     DADLZM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.226"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..439
                     /gene="tnpA"
                     /locus_tag="JNY73_003951"
                     /pseudo
     CDS             1..439
                     /gene="tnpA"
                     /locus_tag="JNY73_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(557..1501)
                     /gene="hypT"
                     /locus_tag="JNY73_003952"
                     /gene_synonym="qseD"
     CDS             complement(557..1501)
                     /gene="hypT"
                     /locus_tag="JNY73_003952"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY8049757.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQEAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTPPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1846..2565
                     /locus_tag="JNY73_003953"
     CDS             1846..2565
                     /locus_tag="JNY73_003953"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY8049758.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2631..3929
                     /locus_tag="JNY73_003954"
     CDS             2631..3929
                     /locus_tag="JNY73_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8049759.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1060 a          914 c         1037 g         1202 t
ORIGIN      
        1 gcgcacaccc gatggaactg taaatatcac atagtttttt gcgccaaaat accgaagaca
       61 ggtgttctac agagagaagc gtagagcaac aggcagtatt ttgagaaagc tgtgtgagtg
      121 gaaaagcgta cggattctgg aagctgaatg ctgtgcagat catatccata tgcttgtgga
      181 gatcccgccc aaaatgagcg tatccggctt tatgggatat ctgaaaggga aaagcagtct
      241 gatgctttac gagcagtttg gtgatttgaa attcaaatac aggaacaggg agttctggtg
      301 caggggggac tacgtcgata cagtgggtaa gaacacggcg aagatacagg attacataaa
      361 gcaccagctt gaagaggata aaatgggaga gcagttatcg attccctgtc cgggcagccc
      421 gtttacggac cgtaagtaac gaagttggat gcaaatgtca gatcgtgtgc gcctgttagg
      481 gcgcggctgg taagagagcc ttataggcgc atttgaaaaa cctccggcta tgccggagga
      541 tatttattgc acgtcgtcaa ttgcggcgtg ctggctcttc cattgatatc tgctggatta
      601 aatcgtctgg catatgttcc tgtaaaatac gccagaggtt ctcggcggtt ctattcagtc
      661 gggtgttcat tcggtaaata taagcctgaa tggggactgt caatttagca gcatcaaggc
      721 atatcagacg tttggtctgt agttcatcga cgatcgacca gatcggtaac caggcaatgc
      781 cgtatccgtt gagcgccata ttctttaata actcgctcat cgaagagata aatatcgtgc
      841 gcccggtaat acctccaacc tctgccagat gacgattgac caacctgccc atataagatg
      901 tggcggtata gttgagcaaa ggaacctggg gctgagtgat atcgaataca gggtgcccct
      961 gtgcgtcggc ggcacatacc gggtacaact cagattcaaa cagtttcagg cagcaaaaag
     1021 gcgattgcat aagatcttca tcgcgaaacg aaataataaa gtcactcttg ccttcgcgta
     1081 acgtatttac ggcctggaca acatcaatgg cttcaacatg atagacaaat tctccgccat
     1141 aggcggtaag tgaatgcacc agtttgggta atacgctcag ggatagtgag tgcgcggctg
     1201 caattttaat gttgggaacg ccgagtaaac tctggccgtt taactccccc aaattgcatt
     1261 caagctgctg taataagctg cgagtctggg aatgaaagag cttgccctct tcggttaatt
     1321 gtaaaggggg ggtcgtgcga tcgaataaaa caacgccgat cgctgcctcc agcgccttaa
     1381 ttcgtcggct gaatgcgggt tgcgaaagat tacgctcttt tgcctcctga gaaaaatggc
     1441 gacaggcttc cagtgtcagg aaatcgtata accattttgt ctcgatattc ttcattacca
     1501 tggcacctgt attttcaatc caaagacata ataaaacatc ccggcaataa aaataatgtt
     1561 ctcaattagc acaacctggc ctttatcatt cgttttgtgc ataacccctt catttccggt
     1621 attgacgttg atatcacatt tcgccaaaaa aacgaaattt ttccgtcaga taaccagaat
     1681 acatcatcac tatggtgaat aagcggaatg ttccatagct gcatcatgcg tgtcttgcat
     1741 cgccttgtac aaccttgcat ttctcatcgc ccatttcgac tgttaaaaaa gagagcagca
     1801 gttatttacc gcgtattgtc attaacgttt tgagaggcag gtattgtgaa aggtttaatt
     1861 ggtgtactcg gtggaatggg gccggcagct accgtcgacc tgtttaataa atttgtaact
     1921 tttaccgctg cacaatgtga tcaggaacat attcctttaa ttatttcttc gatcccggat
     1981 attcctgacc gtaccgatgc attaatgcat catgggcgtt caccattacc cgttatgcgt
     2041 gattatatgc ataagctgga agatgccggg gcagaatgta ttgttattcc ctgtaatacc
     2101 gcgcattact ggttcaaaga attaaaagat gcttgtcata ttgatatatt aagtatcgta
     2161 gaaactacga tcaatgaagt tcgcgcctgt ggtaaaaccc gaattggctt gttagccacc
     2221 aatgccacgc tatatatggg tttataccaa aaaggaattg agtcgctggg atttacctgt
     2281 gtcagccccg atgccgacgg tcagaaaaaa gtgatggaaa gtatttattc cttgaaagct
     2341 ggcaatatag cgcacgcaca aaagttgatg aatgagcagg ctgaaatatt attttcccgc
     2401 ggtgcagaaa tccttgttct tggttgtaca gaaattccgg ttattctggc gcaagccgtt
     2461 aaagagcagc cttcccgcta tattgactca acggcgtcac tcgttcgtgc cggaataaaa
     2521 tggtacgaaa atcgtgtcgg taaacattat cttttaaccc aataatcatt taaatcgcag
     2581 ccagaaagtt attcgctttt aactgaatta tatttataac ggagaacatt atggtttggc
     2641 tggaaattat cgtagtactt ggtgcaatat tttttggtat tcgccagggg ggaatcggta
     2701 ttggtttatg tggcgggctt gggcttgcca ttctgactct gggatttggt ctgcctatgg
     2761 ggtcgccgcc agtcgacgtg atcctgatta ttatgaccgt tgttgtggcg gcctcagcct
     2821 tacaggccgc aggcggaatg gattatctgg tacgcgtcgc tggtaatttt atgcggcgta
     2881 acccgaaata tatcaacata atcgcgccga ttatcacctg gtctatgacc attatggccg
     2941 gtactgggtt tattgttttt tccacgctgc cggtgattgc tgaagtggcg aaagagtccg
     3001 gtatccgtcc ttcccgcacg cttgccggtt cggttgtcgc ttcgcaggtt gcgatttccg
     3061 gttcgccgat cagcgccgca atggcggcta tgctcactat tatggaggta aacggtgtca
     3121 gttttattca agtgatgtcg gtatgtcttc ccacctcttt tgctgccgcg atggtcgccg
     3181 cttttattgc ttcccgacag ggatgtgagt tgcaggatga cgaggtctac cttgagcgcc
     3241 tgcaaaaagg actggtgcaa aaatacgaga ataatagcag catcaagcca ggtgcgacgc
     3301 tctccgtcgg attgttcctg ctggcgacgg tcgctattgt tattcttgct gccttccccc
     3361 aactgcgtcc gggttttgat atcggtaaac cgatggaaac acgcgatatc attattatct
     3421 gtatgctctc tgccgcctgc ctgatggtgg tgctgtgtaa gacggcgacc gatgccatta
     3481 ttctcacctc cacattccgt gcggggatga gttcgctggc ggtaattctc ggcattgtaa
     3541 ccttaggcac cacttttatt gatgcgcatt taactgagat taaagatatt gctggtgata
     3601 ttttgcaggc gtatccgatg ttgttggcgc tggttctttt tggcacttgc gcgctgctct
     3661 actcgcaagg tgcgaccacg ccgttaatta tcccgcttgc cgtcgcgctt gacgtgccca
     3721 cctgggcaat tctggcgtcc tatgtggccg tcaccggagt atttgtactg ccaacgtatc
     3781 caacatcact ggctgccatg gaatttgata ctaccggcac aactcgtgta ggcaaatatg
     3841 tgctgaatca tccatttatg ctgcctgggt tggggggaat tattgctgga gtggcattag
     3901 gctttgtgat tgcgccaatg attatttagc actatcaaac atcagtatcg atggtttata
     3961 cacaataaag acagatatgg cggaatatag tctgttgtaa gcgccaacaa agaatagggg
     4021 aattccgcca tgtctgaaca tcttcacaat atggtattaa gtgttgtcgt ttaatgattt
     4081 atttcttggt tatgttgtgt attagctgca tctatcgctt tttgatacct gaggtgtact
     4141 ggcaatagcg gacactacca tttgttcttt ttttaagcag tcatctgatg atatttttcc
     4201 ctgaaggctg ccg
//
LOCUS       DADLZM010000222         4145 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLZM010000222 DADLZM010000000
VERSION     DADLZM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.227"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            96..860
                     /locus_tag="JNY73_003955"
     CDS             96..860
                     /locus_tag="JNY73_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAY8049760.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(1353..1937)
                     /locus_tag="JNY73_003956"
     CDS             complement(1353..1937)
                     /locus_tag="JNY73_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8049761.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(1937..3175)
                     /locus_tag="JNY73_003957"
     CDS             complement(1937..3175)
                     /locus_tag="JNY73_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8049762.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(3172..4077)
                     /gene="mph(A)"
                     /locus_tag="JNY73_003958"
     CDS             complement(3172..4077)
                     /gene="mph(A)"
                     /locus_tag="JNY73_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAY8049763.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          858 a         1296 c         1292 g          699 t
ORIGIN      
        1 tgcaccaatc tcgactatgc tcaatactcg tgtgggctct gttgcaaaaa tcgtgaagct
       61 tgagcatgct tggcggagat tggacggacg gaacgatgac ggatttcaag tggcgccatt
      121 tccagggtga tgtgatcctg tgggcggtgc gctggtattg tcgctatccg atcagctatc
      181 gcgaccttga ggaaatgctg gcggaacgcg gcatttcggt cgaccatacg acgatctatc
      241 gctgggtcca gtgctacgcc ccggagatgg agaagcggct gcgctggttc tggcggcgtg
      301 gctttgatcc gagctggcgc ctggatgaaa cctacgtcaa ggtgcggggc aagtggacct
      361 acctgtaccg ggcagtcgac aagcggggcg acacgatcga tttctacctg tcgccgaccc
      421 gcagcgccaa ggcagcgaag cggttcctgg gcaaggccct gcgaggcctg aagcactggg
      481 aaaagcctgc cacgctcaat accgacaaag cgccgagcta tggtgcagcg atcaccgaat
      541 tgaagcgcga aggaaagctg gaccgggaga cggcccaccg gcaggtgaag tatctcaata
      601 acgtgatcga ggccgatcac ggaaagctca agatactgat caagccggtg cgcggtttca
      661 aatcgatccc cacggcctat gccacgatca agggattcga agtcatgcga gccctgcgca
      721 aaggacaggc tcgcccctgg tgcctgcagc ccggcatcag gggcgaggtg cgccttgtgg
      781 agagagcttt tggcattggg ccctcggcgc tgacggaggc catgggcatg ctcaaccacc
      841 atttcgcagc agccgcctga tcggcgcaga gcgacagcct acctctgact gccgccaatc
      901 tttgcaacag agcccgccgt gctagtctgc tcggtgatgg tggagtgaag ccaacccgca
      961 atcgggttat gaatctgcat cgcgattcgc aatcagctgt ctcttgagca tgtcgaactc
     1021 ctgcgatgtt atctcgccgt gctcgtgctg ctacaccaac ttgtccaact catccgccac
     1081 accgctcccc accacctgcg atggccaggt tgtttgctgg gcgcattggc ggagtatcgt
     1141 ctcgacccgg gacacccaca ccggcacaac cttacgggag tgattcactg tcaaagaatc
     1201 ggcccggtgc tctgacgcaa gtatcgggat ggtcaccatt tgtaagccgt agacctgagt
     1261 ggtgatcaag acttcgatac caccgaccgt accggtacta atcgacgacg gtcgtgttcg
     1321 tcgcctgccg cagggactct gcacacctcc gtttacgcat gtgcctggag gagttggaaa
     1381 tcgtcgtgtt cgggaaacat taaacacagg atggcagcga tctgagccag cacatgatca
     1441 gctagctcac catccggatc gacggcccac tgcatcgtcg cgccagcgat gaccgagtgc
     1501 aggagcaact cagctgccgc aggagcacct gggggcagtc gcttgcggat cccctccacc
     1561 accgcgcggt tccgctggat cgcaagcgtg cgtagctccg gcacctggag ctcgtaccag
     1621 gagatgagat agttcaccga gaagtcgttg cgagtgttca tgctccgaac gagcacctgc
     1681 aaaaattccc agagcccttg cggccctgcg cctatcggta tcgcattcag gtaatgccgc
     1741 acctgctcga cgccgcgctc catcatcctc accagcagcg tatcgcggtt ggtgaagcgc
     1801 tggattaacg ctgcgcggga gagccccacc tcctttgcta ctccgctgag cgtgaactct
     1861 atgggaccgc aacgcttcag cactacggtg gcggcctcga gtacctcgtc atcggacttg
     1921 agcttggggc ggggcatcag tgttcacctt ctgtatgggt tggggcggag gctgtggctg
     1981 ccgccgccat tgtagcaaat tgaagacgga gcgagagtag agccacgagc cccgcaaaca
     2041 cggccgatac aacgaggcca gggagcggac cagcaaggtc gacaaacgcg ccggccgcaa
     2101 gcataaccat gggcgaggct gacagcatca ccgccgagac cgtgccgagt acccggccga
     2161 gaagttctgg cggcgtgcgg ttgtagatgg cagcgttgag aatgggagag actgagccgg
     2221 tcagcagtcc cacgagcgcg cccaacaaca tcagcaccgg cacgcctggc aactgtgaaa
     2281 gcagaagcga gcccaccgca gagccacaaa atgccaccgc cagccagttc tgcgctgata
     2341 tccgggcgcc gaccgacgca tgaatggcaa tgccaaggag accaccagcc cccatcattg
     2401 aggagaacag cccgagctct gctacttggc gtcctgcatc tacaaacagc gcaggcatga
     2461 tgacgctgcc gttggcgcca acgatgccca cgaagatcat cactatacca aagagagggc
     2521 gcagcagggg ttcgctccag agaaaagcga cgccggcgcg catggagaga gtcgccgtcg
     2581 tggtcatcgt ccgagcggca cgcgcgggaa gcacccacgc gccgagcaga cctgcaagga
     2641 cggagcagaa cgccgtcagc ccgagcgttg gcgcagcgcc aagcaggccg attgcggccc
     2701 ccccaagggc cgggccacct agaatcgcga cgttcccgat caccgctttc agtgacgaga
     2761 cgcgctcaac ggagagcccg gcgacgtggc cgagtttggg cagctcactg tcctgcgcgg
     2821 ccataccggg tgcgtcgaac gcggcaccga gcaccacgca agcgatcagc ccagtgttcg
     2881 agagggcgcc aacggcatcg agcagtggga tgctcgccat ggccacgccg cccaccacac
     2941 ccgagatcaa tgcgacgggc gcgcgcccga accgatcgac gaggccacca ccaacccacg
     3001 cgccgatgat ggtcgcgatg acgctgctag cggccgtggc gcccgcccag gccgcgctct
     3061 ttgtatgaga caggacgaac catggaagcg cgagggccgc caccgcgttg ccgatccgga
     3121 agagaaaggt cgccgcgaac agcgtcgcga gcgggctata tcgacgttcg ctcattccgc
     3181 tgcggcgagc tgcgccttcg ccgcagcgag gtactcttcg ttacccgagt cgagggcgaa
     3241 gagtgcgtag gtgaccgccc cgaacgcaag gcgctccgcg atgtggtggg cgagccgcgg
     3301 ccacacccgg ccaccggccg cttcatacgt gaggaggagc ttcgcgagcc cctcttcacc
     3361 aaagaccata aggtgcgcgg ccatgtcgat ggcagggtca tcaacgcggg cctcgctcca
     3421 gtcgatcatc ccgctgacgc gctccgtgtt gtcgatgagc acatggccca cgtagagatc
     3481 gccatgcacc accacggaga aatctggcca cgacgaatcg tcgtcgagcc agcgctgcca
     3541 ccggtggagg cgcttgtcgt tcaccacgaa ctcgcgtcgg acgcggtcaa cgtcgtcggc
     3601 caccttctga cgggcctgcg tcggtgtacg gatgagcatc cccgcatcca cggcggcgga
     3661 aatggggacg gcatgcaggg cggcgagcgc ggtcgcgaag ctctccgcga agacctccga
     3721 gtcctgcggc acgacccagt cgggcgtgga cgaaccaggc tggatgacca tcgcagtcga
     3781 gtcttcgagc atgggatagg caacgagctc ggcgttggcc acgcgccagt ccggcaccgc
     3841 gaacggcagg cgattcttga gcattgccag cacccgcgcc tctggttcga ccttcgcgct
     3901 tacctcggct cggcgcggga tgcgcagcac ccaccgacgt ccatcgtcga cggtggcgat
     3961 cacgatccta tagtcgagcc caagctcatt gacagtcagc gggccatgga gcttgagccc
     4021 atgtcgggct gcaagtgcgt acagttggga ggtatcggcg gtcgtgacta cggtcatgat
     4081 tcactcctga gggcttgacg ggtttagcca cctaaatgta acagtcacgt cggttatatt
     4141 caatc
//
LOCUS       DADLZM010000223         4113 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.228, whole genome shotgun sequence.
ACCESSION   DADLZM010000223 DADLZM010000000
VERSION     DADLZM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.228"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            244..1199
                     /locus_tag="JNY73_003959"
                     /pseudo
     CDS             244..1199
                     /locus_tag="JNY73_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1196..2101
                     /locus_tag="JNY73_003960"
     CDS             1196..2101
                     /locus_tag="JNY73_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY8049764.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2098..3168
                     /locus_tag="JNY73_003961"
     CDS             2098..3168
                     /locus_tag="JNY73_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY8049765.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            3304..3981
                     /locus_tag="JNY73_003962"
     CDS             3304..3981
                     /locus_tag="JNY73_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049766.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT         1070 a          969 c         1139 g          935 t
ORIGIN      
        1 tcacttatcg atcaactgat aacggaagtc atactgagtt cacactccag agctgcggca
       61 ctggcggttg attctgccgc cgcaaaactg atgcagaatg cagacaatat cagtgaatac
      121 ctgtcgttac accatcaggg gctgatgcaa agtattcagt cactgcaggc gcatatcacc
      181 agcctgcttg aggatatccg ggaaatagca gactgtcagg agcaggtgac cgctgatgtc
      241 agaatggcca tggaggagat cgatgccaga acccgggaat tactgacggg gtctgcacct
      301 cactggaaga ggagctgaat gactatttca gaagcggtaa acgcaaagaa cagcaaatgc
      361 tggaggaaga aaattcagcg cagccccggg agagaaacgc attcgctttc tttcacgata
      421 ttttcggaac cggaaatcag catgacagga tgcgggactt tgatccggac agccccgaaa
      481 taaaattcag tgatcgcagg gaagcccttg aactgatgac gcaaatcgaa tcgaccgtga
      541 ccagcctgca ccgagaggct gaagcacagt tccggcctga gctggagaaa atcgtcagcg
      601 ggattgaaac aggttttcgt ggcacggccc tgtacgccac agaaaacatt gccggtcgca
      661 tcaatacccg cctggaggat gagggcttta ccgtaaaaat cagttttccg gcagtcagtc
      721 agttacaaac ccggctcgcg gtaaaaacaa atctgagtgc gcttatggag gaaagaacgg
      781 agaccgtcac ccgtcgccgt cggcagcgtg gtttatgggg aaagatttgt ggagcgtttg
      841 gcaccagtga ctggggctgg gaaacctata aagaggatgt gagccgcagt gttatcaata
      901 tcaacaccgt caggaaggaa gttatgtcac tgacccgggc atatttcggg gagctgcagg
      961 catccattga gcaggatatt aaccagcccg tccgccagga aatcgatgcc tttttctgcg
     1021 cattcaggga gaaagttgaa caactgcgta acacgctgat tcagagctct gaagatcata
     1081 aacgcgatca gcaggcacag gaacggctta ccaggcgact tcaggcatta aatgaaaggg
     1141 ttcctgagct cattactgac agtaaggcgc tgagggaaga gctggagaca atgctgtgac
     1201 ttcaccattt attcaacaaa ttgctgataa ccgggtatgt caggtactca cctgtcttcc
     1261 tgaaaaattt gtggttgatt tcgcaaacgg aatcgatgtt gcacaggagc atatccgcac
     1321 ggccggagag cgcacgttct tccggcgttt aaaagaggga ctgaccggcg aaggcgctgc
     1381 acggcagaat gccatcaatg cttcggttgc acagggcgtt gaggcgtccc tgcgctggct
     1441 gacggagctg acaacgtcac ttgccaccac aaactacgcg attacccggg taaacgacag
     1501 ggtcagttca ctggtcagtg atactgccag gttggcgcat tattccgcag atacacggga
     1561 gcagttactc atcctggccg atcaggttca ccataaactg aatcatcttg aagaaaaact
     1621 ccaccgtgtt gaccaggttc agcgggcgca gttacatctt gagcagatat tctcatggtg
     1681 gagcgccggg cgatacgcgt cattttcccc tgccggacgt tgttatgtgg cacttgaaga
     1741 gcttcgctgg ggcgcgtttg gtgatgtgat acgtcagggc gaaacaggcc aggttaacca
     1801 gctactggat attctcagac ataaagcatt aacgcagatg gcacaggaga gtggcggtag
     1861 tgcaaccgtg cgtctgaaca ctctggactg gcttggtggt cagggccggg agcaggcaga
     1921 taacgagtgg catgacgcga ttaactggct gggagactgg tgcagtgaag agcagcatcc
     1981 ggtgatctgg tcaaccacgc aggctgcaga acacttaccg gttcgcatgc ctcgcctctg
     2041 ctctgcagaa cgcctctctg aaagtatggt cgatgaaata tttcagaagg gggctgcatg
     2101 agtacagaaa tgaaaacggg gctggtgctg tccggtggcg gggcggtggg cgcttatcag
     2161 gcgggagtgg ttaaggcact ggcagagtgt gatacacaga tcagcatggt ttcaggggcc
     2221 agcattggcg cattcaatgg tgccattatc gcggcctctc ccgatctgtc agaagctgcc
     2281 gtacgcctgg aagcgctctg ggatcatctg gggaataatc aggtgctgtc ggtaaaccga
     2341 ttggtttact tttcattgct gaaaaaattg ttccaggcaa tgaacctctg ccagatcccc
     2401 ggacgtgcag gagcactgct tacgacgctt cttcgccata tatcgatact caacgggttt
     2461 gacaatctga tggctcagcc gttgttgtca gatgagcccc tgacagcgct gttggatcat
     2521 tatcttgata ctgatgctct ggcagacggg ctaccgctgt atgtgtcgct gtaccccaca
     2581 gaagggggca tgcaggatat tattgactgc attcgtgctg aactgggtgc cggaaccacg
     2641 aaaaacgccg tttttcagca tatccagagc ctgccccgcg gacagcagaa agaggctctg
     2701 cttgcgtcag ccgcgctgcc cctgctgttc cgtccccgtg aggttcaggg gacaatgttc
     2761 ggtgatggtg gtatgggagg atggcgaaat atgcagggaa atacccctgt gacgcctctg
     2821 gtcgatgccg gatgcaatat ggtgattgtg acgcatctga gtgacggttc tttatgggat
     2881 cgccaggctt ttccggacac cacaatcctt gagatccgtc cccggaaaag gctgaaatat
     2941 gcaggtgatg gcgacaacag cggcggtctg ctcagtttca cgttggcaca taccgacgcc
     3001 tggcgtcagc agggctatga agacacgatg ctgacgatgg agcatatccg gaaaccgctg
     3061 gcagcacgtc aggtactgtc ccggtcagaa acggtgttgc agaaaagtct ggagataacg
     3121 gaagaggcag atttggcact gagaaacgcg atggcccgga ttaaataaag acgctccaaa
     3181 gaaacgccac cgcctgtggt ggtgtttttc ctagctggtg ttattgaaaa tattttcaga
     3241 tatatattac ctgagagaaa gttttacccc tcacctgtca attcactcac tggaaattta
     3301 attatgcaat tacctgtaaa gttattaatg agccttatat ctctggtcag cgttattgca
     3361 cgtgccggga aatataaaaa ttatatccgg gatgaaataa aatactggcg atatacatca
     3421 tacaagggtg ggaaatttcc ggaaggtttc actgatgaga aattttccag cgccatttac
     3481 aacggaagaa tatttacaat gaaacgttta cataccctga tgttatttct ggcggttctg
     3541 tttactggct ttaacgtgga agcagcgagc gtgaaacaag cgctcagctg cgacccaaac
     3601 gcccgggctg aacaacctgg agcgtgtcca acaacgtacg agttgtacga aggtgacgct
     3661 gcctacaaag ctgcgcttga caaagcatta aaaccggtcg gactgagcgg catgttcggt
     3721 aaaggcgggt atatggatgg ccctggcgga aacgtaacgc cagtaaccat taacggtaca
     3781 gtctggctcc agggcgacgg ttgcaaagcc aatacctgcg gctgggactt tatcgtaaca
     3841 ctctataacc caaaaaccca tgaagtcgtt ggctaccgct actttggttt agatgacccg
     3901 gcctacctgg tttggttcgg cgaaattggc gtgcatgaat tcgcgtatct ggtgaaaaac
     3961 tacgtagctg cggttaacta atttaaggag tctgtcatga aaacttttat caaaacttta
     4021 ctcgttgctg taactattct gttctctgtc ttcgctacgg cgaaacaagt aaaactgcca
     4081 aacaacatca aatacgttaa tactacagag gcg
//
LOCUS       DADLZM010000224         4105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.229, whole genome shotgun sequence.
ACCESSION   DADLZM010000224 DADLZM010000000
VERSION     DADLZM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.229"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..158
                     /locus_tag="JNY73_003963"
     CDS             <1..158
                     /locus_tag="JNY73_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY8049767.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="JNY73_003964"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="JNY73_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY8049768.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="JNY73_003965"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="JNY73_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY8049769.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1293
                     /locus_tag="JNY73_003966"
     CDS             874..1293
                     /locus_tag="JNY73_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY8049770.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1280..>4105
                     /gene="traI"
                     /locus_tag="JNY73_003967"
     CDS             1280..>4105
                     /gene="traI"
                     /locus_tag="JNY73_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY8049771.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMELWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSGNAA"
BASE COUNT          923 a         1058 c         1288 g          836 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac
     1261 ggacggacag tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga
     1321 caccagtcgc gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca
     1381 gtataacggc gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga
     1441 gaatgtgtac gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca
     1501 ggttgaggcg ctgggctatg aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc
     1561 gggtgtaccg gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga
     1621 agacgcctcg ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca
     1681 cgtcgatcct gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga
     1741 catccgggca tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg
     1801 acccgcttca caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt
     1861 aagtgaacgc aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact
     1921 gccgccggaa aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagccg
     1981 tgagcagctt atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct
     2041 cgatgagctg tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt
     2101 acatccggag aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc
     2161 acaggaccgt ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga
     2221 gcgggtggct gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc
     2281 tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat
     2341 aaccggacgt cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt
     2401 cgaccagggc gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg
     2461 tcataacgta caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact
     2521 gatggccatg aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc
     2581 ggccaccatc atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt
     2641 tgcggccagc gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca
     2701 ggccatactg acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc
     2761 ggaggtaacc atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg
     2821 ggatatgtac cgcccgggaa tggtgatgga gctgtggaac ccggagacac gcagtcatga
     2881 ccgctatgtg acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca
     2941 gggcgaaacg caagtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc
     3001 ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact
     3061 ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt
     3121 tgttgtgccg ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc
     3181 actgaagctg gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac
     3241 ggtttttgcc tccgtcacac agatggcaat ggacaatgcc accctgaatg gtctggcccg
     3301 cagtggtcgt gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact
     3361 tgcccgccat ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac
     3421 attgctggaa accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc
     3481 cattcatctg gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct
     3541 gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga
     3601 aatcaatgcg cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg
     3661 cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat
     3721 tctcgaaggt aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga
     3781 gaaactgaca tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt
     3841 cacggtggtg cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc
     3901 agccgtgaac atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca
     3961 ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct
     4021 gcatgacacg cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttccttct
     4081 cgatgagagc tctggtaatg ctgcc
//
LOCUS       DADLZM010000225         4078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLZM010000225 DADLZM010000000
VERSION     DADLZM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.230"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..79)
                     /locus_tag="JNY73_003968"
     CDS             complement(<1..79)
                     /locus_tag="JNY73_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049772.1"
                     /translation="MTDITANVIVSMSSQLFTMARSFKAV"
     gene            complement(211..2052)
                     /locus_tag="JNY73_003969"
     CDS             complement(211..2052)
                     /locus_tag="JNY73_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY8049773.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2052..3458)
                     /locus_tag="JNY73_003970"
     CDS             complement(2052..3458)
                     /locus_tag="JNY73_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8049774.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQENQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVGPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(3468..>4078)
                     /locus_tag="JNY73_003971"
     CDS             complement(3468..>4078)
                     /locus_tag="JNY73_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY8049775.1"
                     /translation="NTLQETRGGSQKYAADLQNQQWQTIMKNLTPFTPLAEQYVNQLQ
                     NLSSLEGQGQALNQYYNSQQYKDLAGQARYQSLAAAEATGGLGSTATSNQLATIAPTL
                     GQSWLSNQMSNYNNLANVGLGALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSL
                     QTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          930 a         1045 c          920 g         1183 t
ORIGIN      
        1 ctacggcttt aaaagaacga gccatagtga agagttgcga agacatcgat acgatcacat
       61 tggctgtaat gtctgtcatt taatttgctc cagatacaat gaatcgccgc agcgtggcta
      121 cggtgaattt tgggcataaa aaaacccagc cgaagctggg tagttgcgtt ggttatctgt
      181 cagtagttat gtgcttattt aattatttat ctattgttct gctgtaattt ggcttgaaag
      241 tatagggcgt atcgcatttg cagcattatt taacgctctt tcataagctg ccgttcctgc
      301 tttggtgttt gccaaacgaa gaagcatatt ccttgcggct ttggactcat acaaacgcat
      361 cattgcacca aaaccagcct caagccccat tgatacacca agagtcgcag ttgcgccaat
      421 cgtccttatc ctgttggctt gcgattgccc cgtctgagtt actacatttg cggtgtctga
      481 ccttgctgtt tgctgtagaa cttcatgaag agcatcaagc tctttcatgt gctttccaga
      541 aaaaatagtg ttgtaaattt caccgcctga ctgagatttc agcttattaa cttcagtgat
      601 gaacttggct ggagagtcac cggccttttc cgctattttg ctgacgtaag ctgcacgcat
      661 agcatctttc cctttatcat ccagtgcgct ccagattcgt ttcacgtcag atggttttct
      721 gcttaataca acggtattta taagttcagg actggcttca ctgcttgcct tgttgagctt
      781 gttagcaatg tttttattaa gcaccttatt ataaacgttt gcataatcgg aatttgcttt
      841 aaggtatttt gctgcgtctg atgcaccgag gtttttagca actgcgttac gaaggtcttt
      901 tgacattgca ttctctacca tattggtagc tgcttttgcc tggttgggga agaccatagc
      961 atctccctga acattagatc taaatgctgt tctgtgctga cgcaagagat caaacgtaac
     1021 atccaaatca gttgcagggt ttgctaattc ttcacgtagg ttacgcaagg atgtaagcag
     1081 gctttgattg gcagacgtcc caagccgttc ctgtcttgcg atcgctgtat tcagagcatt
     1141 catggtattt gtggtatcaa ctgcggcatt acccatttta ttggtgacgt cattgataac
     1201 agcgccagcg gcatccttcc gcccccttaa cgtggtggta agagatttca ccacatcatc
     1261 agggttgtac tcaccaaaac ggtcaaaata attacttacc agcttactac gcgttgcata
     1321 ttgctccgct cgctttgagc ctgtcccgag caaagccccc tcggcatcct gagttaggcc
     1381 gcgagtgaaa gcatttttcg gcgggataac atcagatgtc attggtgtca cgcccatcga
     1441 ttctgatgtg gcaattttct tcgccacttc tggcgcaata tcacctttta tagccgttat
     1501 tccacgccct attccctttg ctgctgcgga aagaacaccc tgagcggcaa ggttaactcc
     1561 ggcattttta gctgcatttt gtgcgaaatc gcctttctga tttgcggcct ctgccagtga
     1621 tccaatagcc atgcttcctg ccgttccaac tcctggaact aaatacccac caattgtttc
     1681 accggcttgc gcgtaggggt ctgtcggtct gtctactgga cgataaacat catccaaaac
     1741 cttgggtcca ccaagcccct gactgattgc attaatcaga cttgcgccgc cctgcaatac
     1801 gtcaaatggt atgtttacca gaccacgacc agcctgttct gcaatttgcc ctgcactttg
     1861 accaccagtg agccaatcgc cagcttgttg catcaatgat ggttcttccc gtgttggtgc
     1921 attattggcc tgattaactg tttgttgctg aacagcctga ccagcaaaat actcatcaat
     1981 ggcggtgcca atatcttcgg tgctcgtacc atcaggaaag gtaaatgtct taccgtttgc
     2041 agttactttc atcattccac cgtaaattga atgcctgatt ttgaggtata tgatccaacc
     2101 tgattccgtg gttctcctga aggtgtcgaa tcttgtgctg gcgctgcgtc agtattcatt
     2161 gacatatacc gcttaacggc actccccaat gattcacctt ttttaacatc caaccccaat
     2221 atctgaccgc cattacgcga ttgtccaggg ttgccattcg cgctcatcca ctcggcttta
     2281 aactcattaa actgcgcgtt tcgtcgctca aggtttgcca ttgcatcaag ccatcttgcg
     2341 accgtctcag ggttatccat gtcagttggc gcaccctgtc gaacgatctc aacgtcttta
     2401 tccgttgctg ggccgggagg taggaattta agaacctgac tgttaacaag ggcattttgg
     2461 cgaatgcgca aatcacgcaa tgttgtatcg cttccggtaa gttttgcgaa catgttctgt
     2521 gcgttaccga acaaacctgt cgttggtttt tctgctctga actgttgagc aagcgcactc
     2581 atagaattgg ctgagtttga tgatgctgta gcattgttta cagccgtctc gatgcctttt
     2641 tccatgttta ctgacagctt aggtgcttcg ctaatcaact gctgagcctt ttcctgcgct
     2701 tgctgcatct taaacccgaa ctcttgctga tccagagcca agcgttgtgc tgcgatattg
     2761 tgcccagtca ttgctgactg ataggaaagg ttttgccctc tcgcctgaag tgcttctcca
     2821 gcctgattgc tgcggattgt ctctgccagc ctgcctcggt caatttcacg accagccatc
     2881 ttgtcctgaa cattgaagta atcaatcgga ccaagagcag ccattccaag gtgatcaaca
     2941 aactcaccaa atcctgaagg attctgctga tacatctgag caacgctgtt agggccaaca
     3001 ccgacgcgcg ccagctcatt ggcgttgttt tgcagccatg attgcattgc ttctggagac
     3061 gaggccgcaa ggcgtgcgcc agccgctaag gtgccgatag aattacgctg gtcttcatca
     3121 atgaatccca tgcctttacg aacggattca atctggtctg gatattgagt agccaactga
     3181 cgcaaagcac cgcgatcacc agacgcataa gcattagcgt acgcctgctg aaattctttc
     3241 tgccgctgag cctgattttc ctgctgaaac acccctgcaa tacctgaaag gccttgcaaa
     3301 gcagtcagcc caacattgtt agcgcctgaa cgctcaatat cattgttctg cctgataagc
     3361 tgaagcgtat tgccgatgtc atttacgctc ggagcgtttg agttgacgcc gccgatacca
     3421 gctaacaatc cgccatttga tccttgccaa gtagccatga ttacccctta aaacaacgag
     3481 ccaagcaatc cgataccagc accaatgcca gcgccccaag gtgttgatgt tcccaaaagg
     3541 ctggcaagac ctgcaccggc aatcgcacca gatgttccgc cactaattgc agtctgaaga
     3601 cttgatggtt tattggcatt agcagcggca agagctgcgc tttgctgtgc aatgctgctc
     3661 atgttgttgg cgtacgtctg cccggcgttt gcctgacctt gcagcgcacc aagcccaacg
     3721 tttgccagat tgttgtaatt gctcatctgg tttgataacc aagactgacc gagtgtcggc
     3781 gcgatcgtag ccagttgatt gcttgtggct gtcgaaccaa gtccacccgt cgcctccgca
     3841 gcagcaagac tctggtaacg agcctgacct gcaaggtctt tatactgctg agagttgtaa
     3901 tactgattaa gtgcctgccc ctgaccttct aaactggaaa ggttctgaag ctggttaaca
     3961 tactgctccg caagaggcgt gaacggagta aggtttttca tgatcgtctg ccactgctga
     4021 ttttgcaggt ctgcggcata cttctgtgaa ccgccccggg tttcctggag agtgtttt
//
LOCUS       DADLZM010000226         4058 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.231, whole genome shotgun sequence.
ACCESSION   DADLZM010000226 DADLZM010000000
VERSION     DADLZM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.231"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..111
                     /locus_tag="JNY73_003972"
     CDS             <1..111
                     /locus_tag="JNY73_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY8049776.1"
                     /translation="QAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(312..1169)
                     /gene="sitD"
                     /locus_tag="JNY73_003973"
     CDS             complement(312..1169)
                     /gene="sitD"
                     /locus_tag="JNY73_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY8049777.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1166..2023)
                     /gene="sitC"
                     /locus_tag="JNY73_003974"
     CDS             complement(1166..2023)
                     /gene="sitC"
                     /locus_tag="JNY73_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY8049778.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     THKALEDRS"
     gene            complement(2020..2847)
                     /gene="sitB"
                     /locus_tag="JNY73_003975"
     CDS             complement(2020..2847)
                     /gene="sitB"
                     /locus_tag="JNY73_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY8049779.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GNVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2847..3737)
                     /gene="sitA"
                     /locus_tag="JNY73_003976"
     CDS             complement(2847..3737)
                     /gene="sitA"
                     /locus_tag="JNY73_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY8049780.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         1101 a          995 c          988 g          974 t
ORIGIN      
        1 caggcgcagg tcgatcgcaa cgatgagatt tatcgccgtc agcgggagct gaaggaagag
       61 ctgaatagtc tgaaggattt gaattcggtt aggaatttta tcgtggaata acagaagctg
      121 cggcacgtcg tatgcaggaa cgtgccgcgg ttggctggta aactttcgat agtgcgagta
      181 ttgaatgatt tctaggcgtt atcgatttta cgtatttttt gcatgataga attcgtacct
      241 cctcccaacg accttccatg gctttccgga cttctgtagg ctggcggacg atgctgtcct
      301 cggatgttgt gctaattggg tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt
      361 gcgttgatgc tggtaacggt aaacgtcatg atgaacacga ccgcgaataa gacgacaatc
      421 gtcggagctg gtgcgctgtc gataaaaaag gacagccaga caccagtcat tgacactatt
      481 accgatatgc cagtcgccag taggagcgca atatgaaagc gttgtgtcag gagtactgca
      541 atggcgccag gcgcaatgag cagagaaata gagagaatga tacctactgc tttcagcgtc
      601 gccacaatgg tcagggaaac catgcacagc agcccataat gcagccatct cgtatgcaaa
      661 ccgcttacct gcgcctgttg ataatcaaag ctgaacagca aaaaatcccg ccattttacg
      721 ctaataacaa gtgtaaccag cccggcaata atcaccgtct ggattatatc gccgatggtt
      781 atcccgagca tgtcgccgaa aagaatatgg tcaagatgta cgtctggttt aattgcaata
      841 tacaagatca gacctgcggc aaacatgcca gaaaaaacaa tccccataac tgtatcttgc
      901 ttgattcggc tgttgtcttt caggtatccc gtcgccaccg cgcagaatac tccggcgacg
      961 aatgcccctg tagccagagg taaccccaga atccaggtaa gcacgatgcc aggaaaaacc
     1021 gcatgactca tcgcgtcacc catcagtgcc caacctttca ggaccagaaa gaccgacagc
     1081 agcgcacagg gtatggaaac taccagtgaa atcagcagtg cgtgactcat aaaggtgaac
     1141 tgcagtggct ccagtaataa tgccatcatg agcgatcctc cagcgcttta tgcgtacgcc
     1201 gacgatttgc caaaagtcca tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg
     1261 tctgcgccac cacgatgatt ccacctgtcg cgccgtccag aaagtaactg acccacgcgc
     1321 cgaggaaact ggttacactg ccaatagtta cagcaattat gagcaggcgg ggaaaacgat
     1381 cggttaacaa ccatgctgtt gcaccgggag tcaccaccag acagatcacc agaaaggcac
     1441 caaccgtttg cagcgccgca acggtcgaaa ccgacagcag ggtgaagaag aggattttta
     1501 atctgcccgg atgtaatcca atagcacggg cgtgattctc gtcgaaaaag gtcaccataa
     1561 gatctttcca tttgaaaaaa agcacgatga tcgagagaat gccaatgata gtgagttgca
     1621 ggatatccgc cggatcgatt gccagaatat tgcccaggac gatggtctga atgttcacgg
     1681 atgtcgggtt caatgacacc ataaacagcc ccaggccaaa aaaggacgag aagatcaggc
     1741 cgatgatggc atcttctttt aagcgggtgc gttggttaag aaacagcatg ctgcccgctg
     1801 ccagtccgcc ggaaaagaag gctcccagag aaaacggaag ccccagcata tatgcccccg
     1861 caacacccgg gacaatagag tgcgacagcg catcgccaat cagcgaccag ccttttagca
     1921 tcagatagca tgacaggaaa gcgcagagac cgccgaccat tgccgagacc cacatcgcat
     1981 tgagcatata ttcatagctg aagggttcca gtagcacgtt catcttgctt ccctctgcac
     2041 cgctgacggt cgatgtgcca caaaagggcg ttcatcgtca gtaataatgc tttcttccga
     2101 gccgttaagt gtgacatggc gcagtacgcc gctaaaagcc agctccaggt tctcggctgt
     2161 aaaagtggtg tctgtcggcc cgcttgccag gactgtgcct ttgaccatga ccgtataatc
     2221 gcaaaatgtc gtgactgagc cgagattgtg cgttgagaca agcatcgttt tgccttccgc
     2281 gcgtaactcc ctcaacaggc tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa
     2341 aggttcatcc agcaggatca cattaccctg ctgtgcaatc gctctcgcca gaaagacgcg
     2401 tttcttttgt ccaccggaaa gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac
     2461 acgttccagc gcatcggtga caatctgacg atccctcttt ttggctatac gcaacatacc
     2521 catatgccca tagcggccca tcatcaccac atcttccacc agtacaggaa atgaccagtc
     2581 aacctcttct gactgaggaa cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg
     2641 aatacccaga acagatatct ttccgctggt cagacgcaca aatcccataa tcgctttaaa
     2701 caacgtcgat tttccggaac cgtttacccc aaccagagcg gcaatagatc cacttggtac
     2761 tgtgaaggat gcatcgcgca gtgctgtgtg cccgttacgc caggtgacag taacatcatt
     2821 aacgacaatg cctgcagatt gcatcattat ttctcccttt tcccggcttt aattccctgt
     2881 accagcgtac tggtagtaac cttaagaagg tcgatatacg taggtaccgg gccgttttct
     2941 gtgctcaggg aatcgacata gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc
     3001 tgacgcgctg gtttatcgga aatcgtactc tcgctaaaga ctgccgggat atgatttttc
     3061 ttaactatat caacaacctt acgtacctgc tgcggtgttc cttgttgatc ggcattaatc
     3121 ggccacagat aaagctcttt tagccccaaa tcgcgtgcga gataagaaaa agccccttca
     3181 ctggtgacca tccatcgctg attctcaggg agttccgtaa tctgcttacg caggggggca
     3241 agggtttggg taatcttggc tttataagta tcggcattac gttggtaggt ttgtgcattt
     3301 gccggatcgt attttatcaa cgcatcacga atattatcga cgtaaatcag agcattatct
     3361 ggcgacatcc atgcatgggg attaggtttg ccctcatagg gtccttcggt gatccctact
     3421 ggcgtcacac ccgaagagac aattacttct ggaaccccat tgagatgctg gtaaaagcgt
     3481 tggaaccaca attccagatt cataccattg gcgagaatca gttgtgcccc ctgcgcacgt
     3541 ttaatatcgc caggggtagg ctgatactca tgaatttctg caccaggctt ggttatggat
     3601 gagacttctg cagcatctcc agccacgttt ttggccatat ccgcgatgat ggtaaatgtt
     3661 gtaatgacct tgaatttttc agttgcactt gcctgaaatg cgatcgagca ggtgagggcg
     3721 aggcacccca agagcatggt tacttttttt attgagtgca taatttagta cctatagtga
     3781 gcaaaattag aacaaacata gcaacggcta tgttttatag cataacctat acaaaatgaa
     3841 aataattatc attaacagct ttgaggccta aaacagggat taggttgttt cctgctggcg
     3901 acatgatatt gaggaaaaaa gtagagcagc tattaggctt tgaaatgact ttatgaggaa
     3961 aattgttgat ggcaaaaatg gggcaaaacg ctgtataagg ggcaaaaaag agtgggttat
     4021 cgtagcttat tgttgtcgct gataatgttt aacatatt
//
LOCUS       DADLZM010000227         4010 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLZM010000227 DADLZM010000000
VERSION     DADLZM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.232"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(247..1605)
                     /gene="plaP"
                     /locus_tag="JNY73_003977"
     CDS             complement(247..1605)
                     /gene="plaP"
                     /locus_tag="JNY73_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY8049781.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGSIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1595..1675)
                     /gene="yoeI"
                     /locus_tag="JNY73_003978"
     CDS             complement(1595..1675)
                     /gene="yoeI"
                     /locus_tag="JNY73_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY8049782.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(1873..2802)
                     /locus_tag="JNY73_003979"
     CDS             complement(1873..2802)
                     /locus_tag="JNY73_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8049783.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2848..3672)
                     /locus_tag="JNY73_003980"
     CDS             complement(2848..3672)
                     /locus_tag="JNY73_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8049784.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3755..4009)
                     /gene="yoeB"
                     /locus_tag="JNY73_003981"
     CDS             complement(3755..4009)
                     /gene="yoeB"
                     /locus_tag="JNY73_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY8049785.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1091 a         1039 c          935 g          945 t
ORIGIN      
        1 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
       61 gaaaacataa attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga
      121 atgcagacca ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga
      181 taaaaaagaa taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa
      241 agatgattat gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca
      301 agccaggtaa atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc
      361 caggttaacc cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg
      421 gaagtgatct ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga
      481 caggttaaca aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac
      541 caggtcgaag ttgattgcca gcagggcaat cgagccaacc aggatgatgt tcatcgccgg
      601 agtacgccat ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc
      661 cattacgtac atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt
      721 aatggtggag aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat
      781 ttcaggctgt gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag
      841 gaagtaagtt gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg
      901 cgggatcacg cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc
      961 ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc
     1021 agaccagaac ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata
     1081 aacaaccatg cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt
     1141 gaagttcgct acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc
     1201 caccacaaac atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag
     1261 cagaatgttg atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa
     1321 gccgacagtc gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata
     1381 gcggcgaacc agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc
     1441 ataggctgtc ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag
     1501 cgtcatcggc tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt
     1561 acgcaattcc acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta
     1621 cggtgataac cgtcgcgtaa gcaaaaaatt tgccccattt ttttggattc ctcagcgaca
     1681 acaactgtcg atttttagta aatatctatc cggtacgaag cccggcctct tggtatgaat
     1741 gattggtttg aagcaaaaaa taaccgacgc taattaaacg tcggttttta gtcatttttt
     1801 gacagcggcg cattgtgcct aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat
     1861 aataattttt ctttaaatgg ctgattttcc gtcatcagat tttgcataaa caccggcgat
     1921 cgcactattt gctaaaattt catctcgcca ccagccgctg gcaagccctg cggtctgttc
     1981 attccagcca atccagacag gttcaaagag agtttgggcg accactttct tctcgattaa
     2041 cgcgccacta tcgagaaaac gttgcgccag ataccgtggt aaatagccgc agccaaggcc
     2101 gctaatttgc agctccagtt tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc
     2161 gagaagttgt gaggccgtag aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg
     2221 atatcgttta atgatacgac ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg
     2281 cgcgaccgca aagacttgtt caagatcgcc caaccgcgag aaaccaaact cactggaaga
     2341 tggtggctca tgcatcgcgc caacgataat atccgctcgc ccctgagtca gggcatccca
     2401 ggaaccaccg agtacgccat tgataaactt caggcgcgtc acgctatgat gttgataaaa
     2461 agcttcaatg agaggcgcaa gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag
     2521 ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc gcacggtatg
     2581 cagaacttcc cgccctttct ctaataacat ttttccggtg cgggtgaatt tagcgcggtg
     2641 tccgctacga tcaagcaatt gaatatttag gtcgctttcc agcttgtgaa cggtgtaact
     2701 cagagcggac ggcgtcttgt agagtttggc tgacgccgcc gcaaaactgc cctctttttc
     2761 tatggcatcg aggatcatca acacatccag cagtggtttc atacacgccc ccttgcagtg
     2821 catgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg gatcgggata
     2881 ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct tacctttgcc
     2941 gctgtccaga ctatttctga actgcggagg ttgcagcccc agtaaacggg ccatctgcgg
     3001 atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt gtccgccttt
     3061 aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga ctaaattaac
     3121 accatgttca ccatcagggg cggtttttcc ggcaaagaag cgtcccggat gacgtcccgg
     3181 tccgaccagg cccgcaagac gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca
     3241 gtcttcgagt tcttctaata ctcgtccact gttggttacc ggattacgcg gggtagtttc
     3301 tttaaccgtg ccttgcgcgt cgccatagac agatgttgag ctggtaaaaa taattcgggg
     3361 aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat agaactcatc
     3421 gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcatcggcat ccataagggc
     3481 atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat agctatcaat
     3541 gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca
     3601 gcctcgcgct gaaagtgaca tcgccagcgg catacctaac caccctaacc cgacaattgc
     3661 gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac gctagcgtat
     3721 caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga catgctgcaa
     3781 tgagcagtga atcatcagta accgcgtata ccagacggtg ctcctctgta atgcgtcggg
     3841 accagaaacc tgacaaatta tgtttcaggg gttctggttt ccccttacct tcaaatggtg
     3901 ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta tctgtttcct
     3961 gccagtacag ataatcatcc catgattcct cagaccagat tagtttcact
//
LOCUS       DADLZM010000228         3993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.233, whole genome shotgun sequence.
ACCESSION   DADLZM010000228 DADLZM010000000
VERSION     DADLZM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.233"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(160..876)
                     /locus_tag="JNY73_003982"
     CDS             complement(160..876)
                     /locus_tag="JNY73_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY8049786.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQVGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFV
                     MRTRLEQKAAFSKQSIAKVD"
     gene            complement(873..1238)
                     /locus_tag="JNY73_003983"
     CDS             complement(873..1238)
                     /locus_tag="JNY73_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8049787.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            complement(1239..2225)
                     /locus_tag="JNY73_003984"
     CDS             complement(1239..2225)
                     /locus_tag="JNY73_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY8049788.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHMKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTSTSILKQRTKNLAPLPHAHQQQNPPQEETVVS
                     IAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(2299..2577)
                     /locus_tag="JNY73_003985"
     CDS             complement(2299..2577)
                     /locus_tag="JNY73_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049789.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            2518..2703
                     /locus_tag="JNY73_003986"
     CDS             2518..2703
                     /locus_tag="JNY73_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049790.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(2745..2957)
                     /locus_tag="JNY73_003987"
     CDS             complement(2745..2957)
                     /locus_tag="JNY73_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8049791.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(3160..3378)
                     /locus_tag="JNY73_003988"
     CDS             complement(3160..3378)
                     /locus_tag="JNY73_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049792.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(3552..3887)
                     /locus_tag="JNY73_003989"
     CDS             complement(3552..3887)
                     /locus_tag="JNY73_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049793.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
BASE COUNT          952 a         1068 c          878 g         1095 t
ORIGIN      
        1 catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt cctgtgccat
       61 ttttcaggca taaaaaaacc gctcggcggc gggttttgct tactttacca tcgcgtacaa
      121 aatcggcaaa gtatcagatt tgcatgaaat atatgccttt taatctactt ttgcaatact
      181 ttgctttgaa aatgccgctt tttgttctaa acgtgttctc atcacaaaca ataaagcctc
      241 actatccagt cggtgaaaaa tgtgtttcat tacaacccag tgaccagtaa atgttttgga
      301 ccagtttttg gttgttactc ccgccagtaa tgccagctcc tgatattcat aaccttcccc
      361 accaacctgg tattcataac cttccccacc aaaaagttct gcttttactg cctgcgccgc
      421 cagccagatt aattttttca ggcgttcctg cgttttccct gcaatttttc tggtaccgga
      481 ttgagcatta aattcattcc acgcccactg tgttatcgcg atctgatatt cccaacaaat
      541 actcccgctg taacaccaca acaaccaggc tttatgatgt tcttcaagag acagaacagc
      601 ccgccgccac gatgatgtcg aaaactcaac cggactgacc agaggaattg acgtcccctt
      661 cgccagcgat tgctttcccg ggattggtgg attatcccgc gttatcattt ttccagtcac
      721 ttcatcgcgg taccggattt ttttacgcct gtaacgccct gtatcgaaca tggcattctc
      781 ttgccaggct tcaagctgac cttttgttgc cccactcaaa tcagcggtgg cgataatgag
      841 ctgctcacgc acaaactgta aatactggtt attcatgcgc accccagttc tgtgattttt
      901 atccccagcc gcccaccagg aacgagctga ccgcgcacaa tattgatttc atcaaactgc
      961 tcgtcgtcta taagtagtcc agcatgcgtc agcgcatcca gcggtgcctt caggatattg
     1021 tccaggtcac gacggcgctt atccggtggc tctgcaataa tctttatcgc cagccttccg
     1081 gacaggttta atttcagtcg ctgctggcga acaattagcg ccacatcacg gcgataacgc
     1141 tcaccggctt ttgatacaaa atatgtgctg ccacgacgtc gccagtaggt gttcaccgtt
     1201 ggcgggtaag gcaaaacaaa ttctatgcgt tcggtcattt atgctttcca cttcagaaca
     1261 cccgaatttc tcgcgtgcat taaaaaacga atcagcaaca acaactgact accgtgtttt
     1321 tcttcaaaat cttttacccc ggcgtgtagt tcgttatggc atttacgaca cagcggaata
     1381 acaaacaaat cgtcagcctt tgttcccatc cctcccagtc catgaccaat gatgtggtgc
     1441 ggatcatctg cctgattgcc acacgtcatg catttctgcg tttttaccca gcgcgtgtat
     1501 acaggcatct cttcccgttg tggtttctgg cgctggagat actgagccgg agattccgga
     1561 tcaacggcaa tgctgaccac cgtctcttcc tgtggtggat tctgttgctg gtgggcgtga
     1621 ggcagcggcg caagattttt tgtgcgctgc ttcagtatgc tggtggaggt ctgctctccc
     1681 ggcacgatgt cgctttcacg gtacattgag cggatttttt ccgcacgcaa ccccagcgaa
     1741 cgacgtaata ccgcttccgg tagcgcgtcc gccacctgat tgcggaccgc ccaccaggat
     1801 aattcagcca gagataattc acgctcctgc gtaccgctta ttgcgtgacc gatgacgtca
     1861 atcatccatg ctgacaggtt ttgatgagca agttgctcga gtgattcgga tgtctggtca
     1921 cgcaactggt tgtcgcagtg ccagcacaac accattgcgc cggtaccata acggtgaatg
     1981 acggtttcgc tgtgatgata atcgccgtgt ggccactggc aggatttcat atggcgcaac
     2041 agccagtcag acaatgcgcc agcaccacca gcagcacgaa tcacccgtgc gttactgaaa
     2101 aacggcagca atgttttgtc ttccaccagc ggctggcgaa cggcaggaac gaccccggac
     2161 ggcagattac gcatgctttt cggttccggc tccaccagta cccgggtatt gtggaatacc
     2221 ggcatggatt cacggcctgg cttaaggacc accagcccga gttccggaac cagaacaggt
     2281 cgaagtaata cccgcacatt acctccagat ccgttgctgg aatgtgcagg acggacgcgg
     2341 tggccgttcg gaataaggga gcctgacgaa gattatccag tgacgataat cgagactgag
     2401 ggctttctta atctcgtatc cgtgtctgcg gtagcgctga atcagccatt cagcctgttc
     2461 ttcggtgcag gggtcgtgct gataccagtc agatttgaat gcatgagaac gccgcccgtg
     2521 cctgctggca ggggcggcag agttatccga attgtaaaat ttggtatcgt gcgccatctg
     2581 ttttctctgc tggcgcagca ggtgccagtt gttcaggctg acggatggat tgtaaaccag
     2641 aacgaccaga aaaaacaaaa cccgccgaag cgggttaagt gcgggtgcgt tgaggatgcc
     2701 tgacacatca gcggtggcga gggatttctc ccccgccggg tctcttactc ctcaggttcg
     2761 taagctgtga agacagcgac ctccgtctgg ccggttcgga ttcgtacctc gcagaggtct
     2821 ttcctcgtta ccagtgccgt cactatgacg gttaaacaga tgacgataag ggcgattaac
     2881 atcgcctttt gctgcttcat agcctgcttc tccttgcctt tcggcacgta agaggctaac
     2941 ctacatgtgt tcagcatgga ttgagcctca gattaatgtt aagcgtcttg caggacgcgt
     3001 aatgttaact ggggcttttc tctatctgcc ttttggtgtt catgcctgag acagatagcc
     3061 tcaagcaccc gcagtcattc tacttaacta agatttccct gcaaaccgtt tttgtccggc
     3121 acagtaaata tccaactaaa ccaatagcgt tcgctgtatt taccgccagt attcaatgca
     3181 catgaccgcc atgaacaccc ctaaaaaaag ggcatttata tgtccaaaca ttaatatcaa
     3241 aacatcaatt ttttccatat accttgctgt gaagatgatg ggcatacatg atgcgaacaa
     3301 ccagaacgca acaaacaaaa actgcaatgc gtttttcatt attcccccta caatcaatgt
     3361 gcaataacat ttaaacacac ctcaatttgg ccggacatat aaatatctaa accagaaaaa
     3421 atcacttaca tagcgttaca aactctttag tctaaatact catcgtaaaa cattccccat
     3481 acttatcagt ccgttccgcg ccaggtagct tattgcctta tctggcaacc tataatcagg
     3541 tttccgcttt ttcagttggc tggtcgttta accgacatag ttaacccatt aatctggttg
     3601 ccggatgctg gtggattttc gcgttttagt tgttcataaa agtgcacagc tttaaccagt
     3661 tcttctgatg taaccgggac tggtggggca gtgaataagg cctgaatttc atagttcggc
     3721 ctgtcgttac aatcctcttt tttcggtaca tatttccagt caccaaccca caactccccc
     3781 tgagagtcca taacaccttt tttcacgtag cgatatcgcc acgctatcgg ctctgcttcc
     3841 agcgatgccc gtgcaatttt gaataactcg ccctctactc gcgccatccc tgaattgggg
     3901 tggcatttcg taatcgctat ttttaatttg gcttcttcga ttaattgttc ttttgttaat
     3961 tcagtcattt tcattaccgc cctggtaatg ctg
//
LOCUS       DADLZM010000229         3977 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLZM010000229 DADLZM010000000
VERSION     DADLZM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.234"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..92)
                     /locus_tag="JNY73_003990"
     CDS             complement(<1..92)
                     /locus_tag="JNY73_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY8049794.1"
                     /translation="MRAPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(331..1680)
                     /gene="lysC"
                     /locus_tag="JNY73_003991"
     CDS             complement(331..1680)
                     /gene="lysC"
                     /locus_tag="JNY73_003991"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY8049795.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2205..3854
                     /gene="pgi"
                     /locus_tag="JNY73_003992"
     CDS             2205..3854
                     /gene="pgi"
                     /locus_tag="JNY73_003992"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY8049796.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1008 a         1050 c         1004 g          915 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg tggtgccctc agtgagccag agagttcagt gtacgggaat
      121 tcgtttgcga gcagaactga taatattcat tttgccttct cttaactatt tatctctttc
      181 gataattaat tagagatgca gataaaaaaa ttaaaggcaa ttattctccg ataaagacgt
      241 tggttaacag agtacaggct cgtttattga tgagcatagt gacaagaaaa tcaatacggc
      301 ccgaaatata gcttccaggc catacagtat ttactcaaac aaattactat gcagtttttg
      361 caccacctgc tcggcatctt cgccgggcac caggaagcac aggttatggc tggatgcgcc
      421 gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg aatacctctt tgccaacgcc
      481 gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc gccagacctt cttccacctc
      541 cacccggcac agtgcggaaa gctccatcag cagagattgc gtcagcaacg tatcgccagt
      601 ggaggttgaa ccggtggtat caagggttaa tgccacgctc acttctgacg tggtgattaa
      661 gtctaccgaa atattatgcc gcgcgaggat gccgaaaact tccgcgagga agccgcgaga
      721 atgcagcata ttcaggctgt gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc
      781 gcggaacagc ggcggatttt cagttttatt gcacaccagc gtaccacctg cgcgtgggtc
      841 tttgctggag ccgacaaaga ccgggatatc gctgcgtact gcgggtagca acgttgccgg
      901 atgcagtact tttgcaccaa aagttgccat ctctgccgct tctgcaaacg cgatttcatc
      961 aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt
     1021 ccagatatca acacgagatg cgtgtaaagc ctccgccagc aaggctgccg tataatcgct
     1081 gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg ctaccgataa atccctgggt
     1141 gatcactaag ccttcattga ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc
     1201 ggctacatct ggctctgcac gaccaaatcg gtcgttggta cgcatcactt tacgtacatc
     1261 aaaccactgt gcctgaacat cgcgttcgcg caggatttcg acaaacagca gggtcgacat
     1321 cagctcgccg tggctgacca gctcatctgt cagcgccgga gacgttgcca gcgccgccgc
     1381 ttctgccaga acagtaatgt tctccagcag acgttcaatc tcttcacgga taacgttcgg
     1441 gtaacgcaga cgttccagaa tggcaaactg gatgttgcga atagcgtcga gtttttcgaa
     1501 tcgctcgcca ggttccagcc cttccgctaa agcgaccagc agattagtga taccagcaga
     1561 agccgagagg actactaaac gcacgttggc atcagaaagc acaatatcag cgctgcggtt
     1621 catggcgtca aaatcagcta cgctggtacc gccaaatttg gagacaacaa tttcagacat
     1681 aactacctcg tgtcagggga tccattttca gccttggcac aagggaagag cggaagacgg
     1741 gtgggcgcag agcgatactt cgctactatt ttcacccaga agtgctccac cacttgcgaa
     1801 acgcccgact gcgaacgctt ctggtgacaa cccaggggat tcagcccctg tagccgatga
     1861 tgaacgtggc cagccgttca atcacctcgg cgatgcaccc cctcaggtgt tatcacagga
     1921 ctggctcctc caacaccgtt acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg
     1981 tagtacattt ataaataaag ggtgagcggg gcggttgtca acgacggggt catgcggatt
     2041 tttcatccac tcctggcggt cagtagttca gctaataaat gcttcactgc gctaagggtt
     2101 tacactcaac attacactaa cggcactaaa accatcacat ttttctgtga ctggcgctac
     2161 aatcttccaa agtcacaatt ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc
     2221 caacgcagac cgctgcctgg caggcactac agaaacactt cgatgaaatg aaagacgtta
     2281 cgatcgccga tctttttgct aaagacggcg atcgtttttc taagttctcc gcaaccttcg
     2341 acgatcagat gctggtggat tactccaaaa accgcatcac tgaagagacg ctggcgaaat
     2401 tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg
     2461 agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca
     2521 ataccccgat tctggttgat ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga
     2581 agatgaaaac cttctcggaa gcgattatct ccggtgagtg gaagggttat accggcaaag
     2641 caatcactga cgtagtgaac atcgggatcg gcggttctga cctcggcccg tacatggtga
     2701 ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg
     2761 ggactcacat cgcggaagtg ctgaaaaaag taaacccgga aaccacgctg ttcttggtag
     2821 catctaaaac cttcaccact caggaaacca tgaccaacgc ccatagcgcg cgtgactggt
     2881 tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca
     2941 atgccaaagc cgttggcgag tttggtattg atactgccaa catgttcgag ttctgggact
     3001 gggtcggcgg ccgttactct ttgtggtcag cgattggcct gtcgattgtt ctctccatcg
     3061 gctttgataa cttcgttgaa ctgctttctg gcgcacacgc aatggacaag catttctcca
     3121 ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct gattggcatc tggtacaaca
     3181 atttctttgg tgcggaaact gaagcgattc tgccgtatga ccagtatatg caccgtttcg
     3241 cggcgtactt ccagcagggc aatatggagt ccaacggtaa gtatgttgac cgtaacggta
     3301 acgttgtgga ttaccagact ggcccgatta tctggggtga accaggcact aacggtcagc
     3361 acgcgttcta ccagctgatc caccagggaa ccaaaatggt accgtgcgat ttcatcgccc
     3421 cggctatcac ccataacccg ctctctgatc accaccagaa actgctgtct aacttcttcg
     3481 cccagaccga agcgctggcg tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg
     3541 atcagggtaa agatccggca acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta
     3601 accgcccgac caactccatc ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga
     3661 ttgcgctgta tgagcacaaa atctttactc agggcgtgat cctgaacatc ttcaccttcg
     3721 accagtgggg cgtggagctg ggtaaacaac tggcaaaccg tattctgcca gagctgaaag
     3781 atgataaaga aatcagcagc cacgatagct cgaccaatgg tctgattaac cgctataaag
     3841 cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat gtaggccgga taaggcgttt
     3901 acgccgcatc cggcaaccgg agcctgatgc caatatcgac attaatgcat agtatgagga
     3961 gttaaataac cttaaaa
//
LOCUS       DADLZM010000230         3955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLZM010000230 DADLZM010000000
VERSION     DADLZM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.235"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..398
                     /locus_tag="JNY73_003993"
                     /pseudo
     CDS             71..398
                     /locus_tag="JNY73_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(428..3340)
                     /locus_tag="JNY73_003994"
     CDS             complement(428..3340)
                     /locus_tag="JNY73_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049797.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGAAPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3343..3459
                     /locus_tag="JNY73_003995"
     CDS             3343..3459
                     /locus_tag="JNY73_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8049798.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3823..>3955
                     /locus_tag="JNY73_003996"
     CDS             3823..>3955
                     /locus_tag="JNY73_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HAY8049799.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     "
BASE COUNT          998 a         1029 c          957 g          971 t
ORIGIN      
        1 acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg aacagtccac
       61 aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc agttccgtca
      121 gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca cgcctgacgg
      181 atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca agcctgcgcc
      241 gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac cgcgcacatg
      301 gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt ccgaaccaga
      361 agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat cctgtcagca
      421 cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat ccgacagact
      481 tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat tccggtgaca
      541 tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc aactcataaa
      601 ccgcgcgcca cacatcaccc tgccttattt taagccccgg gttaagaata tactgtccgt
      661 ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact tcatgttgag
      721 agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaat gcttcaggaa
      781 gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac
      841 ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt gccatctgcc
      901 ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca ggtttcagca
      961 gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc gccgctgaca
     1021 gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggagct gcaccacgct
     1081 ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc agcggagtaa
     1141 gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac acggcaaccg
     1201 ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga gtcttcgttc
     1261 tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc gggaacaggg
     1321 aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct gtacctgcga
     1381 ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat ggcatctcag
     1441 atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa
     1501 gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc agattcagag
     1561 gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc tgctgttcaa
     1621 caagatacac attgtgtgta gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt
     1681 tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg gcctcgccct
     1741 caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta
     1801 ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga accgaaaaga
     1861 ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc atcacaatga
     1921 ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc tcttccctgt
     1981 cgtccagcaa ccggatgctc ccttctgcct gaccgcagct tctgacaaaa tggtgacttt
     2041 cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta gccagtgcag
     2101 tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg tcgtttccct
     2161 gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc ggatgaacta
     2221 tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt ttcagtacca
     2281 gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca tcataacggg
     2341 gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga tgcgactgtt
     2401 tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc tccatgccat
     2461 agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc ccattcccgg
     2521 aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca gcccccagtg
     2581 cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc acctccctgc
     2641 cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca atattctgta
     2701 cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc agaaatgagg
     2761 aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt accagcgccg
     2821 tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg tacagtgcct
     2881 gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca tgagacggat
     2941 tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt tgttgttgta
     3001 agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta taggcatgat
     3061 tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac agcgaaaaca
     3121 gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt ttacgaacat
     3181 cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa cccgggatcg
     3241 tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc cgtcgtgcaa
     3301 aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg aatacccccc
     3361 gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga aggtggggtg
     3421 gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg tcatgatgcc
     3481 ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa ctctgcgctg
     3541 aaattccttt ttttcattgg agcacctgtg ttgttctgag gtgaacatat cacctctgtt
     3601 caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt taaaagatat
     3661 gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat atgaaatatg
     3721 ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca gctttattgg
     3781 gttttccttt tgtctctgct tactaggtgc cagaaacttt aaatgttaaa cgaagggggc
     3841 attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag tcattgcccc
     3901 ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt ggctg
//
LOCUS       DADLZM010000231         3831 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLZM010000231 DADLZM010000000
VERSION     DADLZM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.236"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            22..3816
                     /gene="traI"
                     /locus_tag="JNY73_003997"
     CDS             22..3816
                     /gene="traI"
                     /locus_tag="JNY73_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY8049800.1"
                     /translation="MLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITD
                     SGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAG
                     EESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRP
                     GMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKM
                     PVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTAL
                     KLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEK
                     LARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSM
                     VDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKS
                     ILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTT
                     QFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGE
                     TPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQ
                     TRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEH
                     SVTEFSHSQEAKLAEAQQKAMQNGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTH
                     LNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWENNPDALALV
                     DNVYHRIAGIGKDDGLITLEDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFT
                     KSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQ
                     GASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKG
                     TAHDVFEPKPDREVMSAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKY
                     PQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNG
                     EGLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVRPGAG
                     NGEPVTAEVLAQQQAEEAIRRETERRADEIVRKMAGNKPDLPDGKTEQAVREIAGQER
                     DRTATSERETALPESVLRESQREQEAVREVAQENLLQERLQQMERDMVRDLQKEKTLG
                     GD"
BASE COUNT          839 a         1018 c         1312 g          662 t
ORIGIN      
        1 ctgataaccg gacgtcgtca gctgctggaa ggcatggcct tcacgccggg cagtaccgtt
       61 atcgtcgacc agggtgaaaa actctccctg aaagagacgt taaccctgct ggatggtgcc
      121 gcacgtcata acgtacaggt cctgataacc gacagcgggc agcgaaccgg tacaggcagt
      181 gcgctgatgg ccatgaagga tgccggggtg aacacatatc gctggcaggg cggagaacag
      241 cgaccggcca ccatcatcag tgaaccggac cgtaatgtcc gctatgcccg gctggccgga
      301 gattttgcgg ccagcgtgaa agccggagaa gagagcgtgg cacaggtcag cggggtacgg
      361 gaacaggcca tactgacaca ggccattcgc agtgagctga aaacgcaggg cgtgctcgga
      421 caccctgagg tgaccatgac tgcactgtca ccggtctggc tggacagccg gagccgttat
      481 ctgcgggata tgtaccgacc ggggatggtg atggagcagt ggaacccgga gacacgcagt
      541 catgaccgct atgttatcga ccgggtgacg gcgcagagtc acagcctgac cctgcgggat
      601 gcgcagggcg aaacgcaggt ggtgcgtatt tcctccctgg acagcagctg gtcgctgttc
      661 cggccggaaa aaatgccggt ggcagacggc gagcgactga gggtgacagg gaaaattccc
      721 ggactccgcg tctccggcgg tgaccgcctg caggtggcat ccgtcagtga agatgcgatg
      781 acggttgttg tgccggggcg ggctgaaccg gccaccctgc ctgtggctga ttcaccgttc
      841 acggcactga agctggagaa cggctgggtg gaaacgcccg ggcattccgt cagtgacagt
      901 gcgacggtgt ttgcctccgt cacacagatg gcaatggaca acgccaccct gaacggtctg
      961 gcccgcagcg gtcgtgatgt ccggctgtat tcctcactgg atgaaacccg tactgcggaa
     1021 aaactcgccc gccatccgtc ctttacggtg gtttctgagc agataaaggc gcgtgccggt
     1081 gagacattgc tggaaaccgc tatcagtctg cagaaagccg ggcttcacac gccggcacag
     1141 caggccattc atctggccct tcctgtgctg gaaagtaaaa acctggcctt cagcatggtg
     1201 gacctgctga cagaggcgaa gtcgtttgct gcagaaggaa ccggttttac tgaactggga
     1261 ggggaaatca atgcgcagat aaaacgcggt gacttactgt atgtggatgt ggcaaaaggc
     1321 tatggcacag gcctgctggt ttcccgtgcg tcgtatgagg cagaaaagag cattcttcgc
     1381 catattctcg aaggcaagga ggcggtcacg ccgctgatgg agagagtacc cggcgaactc
     1441 atggagacgt taacgtcggg acagcgtgcc gccacccgta tgatactgga aacgtccgac
     1501 cgtttcacgg tggtacaggg gtatgccggt gtgggtaaga ccactcagtt ccgggcggtg
     1561 atgtcagccg tgaacatgct gccggagagt gagcgtcccc gggttgtggg gctggggccc
     1621 acgcaccgtg cggtcggtga gatgcgcagc gccggcgtgg atgcacagac actggcgtcc
     1681 tttctgcatg acacgcagct gcagcagcgc agcggagaaa cgccggattt cagcaacacg
     1741 ctgtttctgc tcgatgagag ctcaatggtg ggcaataccg acatggcacg ggcatacgcc
     1801 ctgattgcgg ccggtggcgg tcgtgctgtg gccagcggtg acacggacca gctgcaggcc
     1861 atcgcgcccg gtcagccttt ccgtctccag cagacgcgca gtgctgccga tgtggccatc
     1921 atgaaggaga ttgtgcgtca gacgccggaa ctgcgggagg cggtatacag cctgattaac
     1981 cgggatgtgg aaagggcgct gtccggactt gagagtgtga aaccgtctca ggtgccacgt
     2041 ctggagggcg catgggcacc ggagcactcc gtgacggagt tcagtcacag ccaggaagcg
     2101 aaactggcag aagcgcagca gaaggcgatg cagaacggcg aggcttttcc ggatgtcccc
     2161 atgacactgt atgaagccat tgtccgcgac tataccggca ggacaccgga agcacgggag
     2221 cagacgctga ttgtcacgca cctgaatgag gaccggcgcg tactgaacag catgattcat
     2281 gatgcacggg aaaaggccgg tgagctgggg aaagagcagg tcatggtgcc tgtcctgaac
     2341 acagcgaata tacgcgacgg agagctgcgt cgtctctcca cctgggagaa taacccggat
     2401 gcccttgccc tggtggataa tgtgtatcac cggattgccg gtatcggcaa ggatgacggg
     2461 ctgataaccc tggaggatgc ggaaggtaac acgcggctga tttcgccccg ggaggcggtg
     2521 gctgaaggcg tcacactgta caccccggac accatccggg tggggaccgg cgaccggatg
     2581 cgcttcacga agagtgaccg ggagcgcggt tatgtggcca acagcgtctg gacggtgaca
     2641 gcggtttctg gtgacagtgt cacgctctcg gacggccagc agacccgggt gattcgcccc
     2701 ggtcaggagc gggcagagca acatattgac ctggcctatg ccatcaccgc ccacggtgcg
     2761 cagggggcga gtgaaacctt tgccatcgcg cttgaaggca cggaaggtaa ccggaaactg
     2821 atggccggct ttgagtcagc ctacgtggct ctgtcgcgta tgaagcagca tgtgcaggtg
     2881 tacaccgata accgtcaggg ctggacggat gccattaaca atgccgtaca gaaaggaact
     2941 gcccacgatg tgtttgagcc gaaaccggac cgggaggtca tgagtgcgca gcggctgttc
     3001 agtacggcgc gggagctgcg ggacgtggca gctggtcgtg ccgttctccg tcaggcgggg
     3061 cttgccgggg gagacagtcc tgcacggttt attgcaccgg gccgcaaata tccgcagccg
     3121 tatgtggcac tgccggcgtt tgaccgtaac ggcaggtccg ccggtatctg gctgaaccca
     3181 ctgaccacgg atgacggaaa cgggctgcgg ggattcagcg gtgaaggacg ggtgaaaggc
     3241 agcggggatg cgcagttcgt ggccctgcag ggcagccgta acggagaagg cctgctggct
     3301 gataatatgc aggatggcgt ccggattgcc cgtgataatc ccgacagcgg tgtggtggtg
     3361 agaattgccg gtgaaggtcg tccgtggaat cccggtgcca tcaccggtgg tcgcgtgtgg
     3421 ggggacatcc cggacaacag cgtccggccg ggggccggaa atggcgaacc ggtcacggca
     3481 gaggtactgg cacagcagca ggcagaagag gccatccgcc gtgaaacgga acgccgtgca
     3541 gatgaaattg tccgtaaaat ggcagggaac aaacctgacc tgccggatgg caaaacagag
     3601 caggctgtca gggagattgc cgggcaggaa cgtgaccgga ctgccacttc tgaacgggaa
     3661 accgcactgc cggagagtgt gctgcgtgaa tcacaacggg agcaggaagc ggtccgggag
     3721 gttgcccagg aaaatctgct gcaggagcgt ctgcagcaga tggagcggga tatggttcgt
     3781 gacctgcaga aagagaaaac cctgggcgga gactgataca ggaagataaa c
//
LOCUS       DADLZM010000232         3671 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLZM010000232 DADLZM010000000
VERSION     DADLZM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.237"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            344..541
                     /locus_tag="JNY73_003998"
     CDS             344..541
                     /locus_tag="JNY73_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY8049801.1"
                     /translation="MNAHDIEPTILRATGNTHSLDVTFIHEEDLATPWKPEQSSVYAH
                     VNPYGIFELDMETRLPIEVVA"
     gene            complement(623..2332)
                     /locus_tag="JNY73_003999"
     CDS             complement(623..2332)
                     /locus_tag="JNY73_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY8049802.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="JNY73_004000"
     CDS             complement(2764..3483)
                     /locus_tag="JNY73_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY8049803.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1087 a          742 c          660 g         1182 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcactct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaatatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt ggctcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgcga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac gggcaggaat caggctgcat acgcagtgcc
     2701 aaatctaatc gtagttattc taaccagttt tcaagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg
     3601 ggaaggctta gaacgccata ttcagtttcc catagtggaa catcactccc agcttaaagc
     3661 gctcccggtt g
//
LOCUS       DADLZM010000233         3656 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLZM010000233 DADLZM010000000
VERSION     DADLZM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.238"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1247)
                     /locus_tag="JNY73_004001"
     CDS             complement(<1..1247)
                     /locus_tag="JNY73_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY8049804.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDRTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDP"
     gene            1743..2662
                     /locus_tag="JNY73_004002"
                     /pseudo
     CDS             1743..2662
                     /locus_tag="JNY73_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(2749..3264)
                     /locus_tag="JNY73_004003"
     CDS             complement(2749..3264)
                     /locus_tag="JNY73_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049805.1"
                     /translation="MTKLMQFVQRCYYMTNKKMYFILILVFTLLQVCFFALWKARDGS
                     TTSLECTSTLTRNAKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFF
                     TYKIDSNGRISGNAKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSI
                     PGLIINTCAPI"
     gene            complement(3218..>3656)
                     /locus_tag="JNY73_004004"
     CDS             complement(3218..>3656)
                     /locus_tag="JNY73_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8049806.1"
                     /translation="TISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEM
                     FAVAFKDTYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPG
                     ANFIYDVNSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT         1098 a          708 c          642 g         1208 t
ORIGIN      
        1 gggtctttca tctgattaaa tggaacctgc aaagcaccga gacaataaca ttgattttct
       61 tcatttctta ttattccgtg atgactggtt aaaactttag tttctgagtt ataattacaa
      121 acatcgagcg ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt
      181 cgtgcagtat agttaccatt aaaattgatt tcatgatttc cagccagaac gatgacattt
      241 ttattaatac ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg
      301 atatatttat caccaaaaat agtactaaat ctatcgcctg tatgatcccc aagggggata
      361 caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg
      421 tctctcgtat ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa
      481 gccatgtctt cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct
      541 atatgccctg aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca
      601 aaataagccg tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt
      661 ttagaaaaca cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag
      721 acatcccaac gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt
      781 atgatagaaa gcccattaac tttataaatt atgtctgatt tatctccgga gaaattaata
      841 aaataataac catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca
      901 tggttattgt ctggtataat tgtaaagata aacgtgttag tagtattatc ggcaaaacta
      961 ttatattctt gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga
     1021 agactatgtt catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca
     1081 aaatcgttat ctatttgcga atcattatcc agtttatcaa tttcaactat ctttgaacga
     1141 tgtgttctat caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct
     1201 ttatggtttg aagggatgta cttaaatata ttgtcaattg ctttcataaa taacccgcga
     1261 tatttttgac ctgttagtaa aaaataaatg atacatgtct gaaagacata tttacgacga
     1321 tataaaacaa taatacttat atgttcgctg attttaagca ccgagactat ccggtctttt
     1381 actaaataat ggaaaacaat ccatccctgg aagacaattg taaaaaaaac aacagaataa
     1441 gaattaatct taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac
     1501 tcggcacagt aaaccccgct atagttatgc tatacatttg tctttaatga tatctcgtcg
     1561 ttatattaac aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga
     1621 ggagtttaaa gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg
     1681 cttaatttgc atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc
     1741 atgtgctgat ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca
     1801 tttcgcagct cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct
     1861 atgcggggtc aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga
     1921 tggcagacga gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg
     1981 tatcgacggt ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga
     2041 gcaagagcga cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg
     2101 ccgttcccgt tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg
     2161 ccgacataac tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg
     2221 cctagtggcc acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct
     2281 gtggtgttat tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt
     2341 agtcagttcc gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat
     2401 gatctgttac agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg
     2461 ttcgttagcc aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt
     2521 gtcatcattc ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt
     2581 tctggttgga ttaagcgcag tatgttttac attgttctct attgtttcaa tattagaggc
     2641 cggttctgcg aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct
     2701 ggttttttac gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc
     2761 acatgtgtta atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag
     2821 cctcgcattg cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc
     2881 ctttatctga ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt
     2941 actatctatt ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc
     3001 agttaagcca acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt
     3061 agcagagtag tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc
     3121 aagagacgtt gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa
     3181 tgtgaaaaca agtattaata taaaatacat tttcttatta gtcatataat agcacctctg
     3241 aacaaattgc ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc
     3301 cttccccaag caaataatgc atactattta catcatatat aaaatttgca cctggcacac
     3361 agggtaaatg ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct
     3421 cgttaagatt ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg
     3481 gataatccgt tatactgtag gtatctttaa aagccacggc aaacatttca ataataacaa
     3541 ttactaccac aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt
     3601 tttcaacaac atcttcttgt aaatgagaat catcataaga ttgtttactt attgtt
//
LOCUS       DADLZM010000234         3582 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.239, whole genome shotgun sequence.
ACCESSION   DADLZM010000234 DADLZM010000000
VERSION     DADLZM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.239"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            115..645
                     /locus_tag="JNY73_004005"
     CDS             115..645
                     /locus_tag="JNY73_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY8049807.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYNEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            652..885
                     /gene="mbeD"
                     /locus_tag="JNY73_004006"
     CDS             652..885
                     /gene="mbeD"
                     /locus_tag="JNY73_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY8049808.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1464..2582)
                     /locus_tag="JNY73_004007"
     CDS             complement(1464..2582)
                     /locus_tag="JNY73_004007"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8049809.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2700..2789)
                     /locus_tag="JNY73_004008"
     CDS             complement(2700..2789)
                     /locus_tag="JNY73_004008"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049810.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3225..3386)
                     /locus_tag="JNY73_004009"
     CDS             complement(3225..3386)
                     /locus_tag="JNY73_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY8049811.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT         1092 a          675 c          845 g          970 t
ORIGIN      
        1 atgtgattca ggcgctcaca gaggcaggga ttgacgtggt cagggtgacg cgaagcagta
       61 tcagcattgc agacccgaac ggcgggaaga atgtcagact gaaaggagcg ttttatgagc
      121 aatcttttac agacgggtgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
      181 gagaaaatgc tgaacgacga gttcaggaag ctcgaagaat ctgtaaacag ggaactgacc
      241 tcaaacgaat ctttaatcag gaacgctatc aacgaccata catcagccct gaaagaactt
      301 ctggagaggt atcagaaaac gaccgtcgat acgatggacg cacactggaa aaccgtactg
      361 aaaatgagcg tgaaacgctg gctgtggctg attatcgtct cagtactgat gtttgccacg
      421 accgggagcc tgctgtggtg ccagggcatg aagataaacg cgaacatgaa tattctccgc
      481 gagcagaaag agagtctgga gaagctgaac gcgaagacat ggggcgtgcg ttataacgag
      541 gacagcaacg gacgttttct ggtgttgccc gaggggatga aagccgaaac gaactggacg
      601 atggaaaacg ggaaacagaa tgccgtaagg ctggtacagg agtaacagag catgacggag
      661 ctggaaagac atttgctgaa cgcattagag caactgcaac aggactatat gcagcggctg
      721 aacgaatggg agagcgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacgggac
      781 aacgcgatgc tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag
      841 cagacagaac gcttgagcca gttatacagc gagaacagga gataagggac gaacggctga
      901 tacaggaatg cgaacatgcg ttatccctgg agcgtgagcg acagctggaa atacaggaac
      961 ggacactgga tggcccttcg ctgggatggt aatcagagac aggctaaaat gttgtcagcg
     1021 cgtttccggt gcattttttg ggataatggt tggtttgggg gcaccgggaa cgcgcttcag
     1081 tcagaccttg ctgaaaacat aggaacgcgt taaaacgcat acgggcgcga ttctggtgtg
     1141 tcttttgttg ctgtgttttg cctctattgc cagatcgcgc tcattcccct gaacttctgt
     1201 tttcccgctc aggtccccgt cttcttcgct tccgaaagca tgaaaaaaat ccgtttcagc
     1261 tgtctctggc tggcaattcc tgcgtgattc acatggctgc atagctatgc atgtggttaa
     1321 aatttaccgg gtgcgatcgc ggcagttttt cgggtggttt gttgccattt ttacctgtct
     1381 gctgccgtga tcgcgccgaa cgcgtttcag cggtgcgtac aatgagatgt tatggtaaat
     1441 tcaagtctta gattttatga tgtttaatta gctttttttg cgtttataat gttcatcttt
     1501 tttattaaaa ttgattcaat ctttgatgtg ggtattaata ttttctttga caaatagaaa
     1561 gttaaaaaaa acaccattaa tagtataaat aaatacccaa aaacagaagc gttattaatt
     1621 tcaattattg atgttaatct gtatattact acatgctgaa tcaacatgga tatgtagatg
     1681 catttagcat atgagttgaa aaaatagagg ttaaatttct tattaattat taatgctgct
     1741 ttgtaaatga taatgcttat gcacattaaa ggtattattg ataactggtg attgactatg
     1801 tttgttttga actcattagg ccctattaat aatactgtca atatacatat aacaaccgat
     1861 agaataactg tggtgttttt ttgtaattta tttttgtatt taaatgcaag catcccaacc
     1921 ataaactcag gaagtcggca agcaaaaaac caaaatggga agtttaaaaa tgatgatgag
     1981 atatttgata tcgggaatgt tgtaattgtt atcacaaata aaaaaaatat gttacgttta
     2041 aatgtaaagg tattcataac gtgaaaaact atcggtgata tcacgtacag cataataacg
     2101 cacccaataa accactcgcc tgtaaggaaa agaaaatcat aagagaaatg ggcgtttaat
     2161 ataccatcaa gggcaaaaaa tactggaatt attctagtgt actggcctcc tgttgtcatt
     2221 attgatacga gagggactgt attaattatc tctttattta aataagacaa taaaattaag
     2281 agtaaagaga taaaaaagta agtgataata taaggagtaa ggatccttac tcctttgtca
     2341 aggaaataat caatggtgtt ctttctatct agtgaaataa atgccaaata tccagatata
     2401 aaaaaaaaca agctcacacc tacctggcca agaacccctc cataaaatga tggtatgaaa
     2461 gatagcctgg tttggctaaa tatgtagcca taatgagata taataacaaa aatgcaggct
     2521 gttactcgca atgtatcaat tgacggatct ctattagaca taacctttat ttcgctgttc
     2581 aaattatcgc ctttagttga tgatgtaaaa ttaacgctat aaataagcta cacattctag
     2641 agcagcgaaa ggatgattca agtattttta ataataaaac gcattgataa tgtttatgtt
     2701 cagatggtgc tgatagcttt gtcatgctgt tccactgatg ttgataacga cagagacttt
     2761 tgtccttttc ttgttagatg ctgcctcaga ttcggattat ggcgttaaag gggtttgacg
     2821 ctcaaccgaa cggtttcaca cgttaagagc tttttgtctg ataaatcaaa atataatgga
     2881 cattgaaaaa cgccctcaaa acggtgtctt tttaatcgaa gattggcaca aaacggacac
     2941 caccgtccat tagtcgattt tttagacggc ttttctcgcc taacgtgagt tttcgttcgg
     3001 ttgagcatca gacccctttt atattgtaga tttctatttt tagacagttg gaacaggaca
     3061 attaacaaca gatctacttg agatcatttg cttgtcgtcg tagtctcttt cccagaaacg
     3121 aaaaaaccgc cctgcaaggc ggtttctcga aggttctcag agcgaccaac tctttgaacc
     3181 aaggtaactg gcttatgagg agcgcagtaa ccaaatctgt cctttcagtt tagccttaac
     3241 aggcgcataa cttcaagact aactcctcta aatccgttac caatggctac cgccactggc
     3301 gatttgtcgt gtctttccgg gttggactca agttgatagt taccggaacg ggcgtggtag
     3361 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     3421 ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa
     3481 ccgaaaggca ggaacaggag agcgcacgag ggagccacca gggggaaacg cctggtatct
     3541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cg
//
LOCUS       DADLZM010000235         3541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.240, whole genome shotgun sequence.
ACCESSION   DADLZM010000235 DADLZM010000000
VERSION     DADLZM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.240"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..221
                     /locus_tag="JNY73_004010"
     CDS             <1..221
                     /locus_tag="JNY73_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049812.1"
                     /translation="ANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGVG
                     PGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(862..1134)
                     /locus_tag="JNY73_004011"
     CDS             complement(862..1134)
                     /locus_tag="JNY73_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049813.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            1223..1402
                     /locus_tag="JNY73_004012"
     CDS             1223..1402
                     /locus_tag="JNY73_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049814.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1454..1648
                     /locus_tag="JNY73_004013"
     CDS             1454..1648
                     /locus_tag="JNY73_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049815.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(2447..2783)
                     /locus_tag="JNY73_004014"
                     /pseudo
     CDS             complement(2447..2783)
                     /locus_tag="JNY73_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1131 a          760 c          693 g          957 t
ORIGIN      
        1 tagccaattt tatcgcatta caaataaatg gttcaaaaac aggatttgcc gcatctatta
       61 caggttttac tcaaacaata agcgctggta caatcagttt cctgtcagta accgtaagcg
      121 acaacggtgt tggtcccggg actactttag gtcttggttc actaataact tcattactgt
      181 caatagccgt aatcgccatt gcaggaaata ataaaaagta agtgtactct gactgcgtat
      241 gtatctactt tgtgtaatgc aggtgtctat tgaaaataat tcttttcgtg ggcatctgtt
      301 gtataaaata aaaaattatc gtaagtactc tatctgcgat gtcttgtgaa aattatcctg
      361 tctggcaaca atcgcgtcca tctgtattaa tggacgcgaa cgatgaactc tcaaacaaca
      421 ctagatatca ccggctttcg cacttatttg ccagaatcat tacgtctcag attcgacgag
      481 caattatctg tccgggctat cgcacagcag ttaagactca gttattcccc cattcacaat
      541 ctctttcaac gaattaacgc ctcaggtatt gtctgaccat gacccgagtc cctttcattc
      601 gcccaaccga taccatactt tatgccaaca gaaagaatga atgaaccgcg cctaaaatca
      661 gcaaggacac atggcacaaa gtgcgacatg tctcacattt aaccccagtg ggggcaacaa
      721 gtataaaacc gttttttgat taagtggtcg aatatgaaaa ggggaaggtt tatgtgaaaa
      781 atacctgtgt tttctttgaa aacacacaac agtatcaggt tatttatgtt tatacagtgc
      841 gttccaaact gacctgaata attacgcaag ttgcaacaat aaaacatttt tgccgtagtg
      901 tgaggtactc cggtaaattg ctttccaggt gcagacaatg actaacgaag aactacaggc
      961 aatcgcatta tccatgcttg agcgccagcg cgaaaaagta caccagcaac gagagtcttt
     1021 gcttaaccca tctccattca ccaggccaaa acgattggag cttgaagcat tcttggacaa
     1081 atacccacgg cggcttaaat caggcaaaac cagaccacca ggctgatagc tcacagtttg
     1141 ttctccgatt caataaaacg gataagaacc atcagcgcgg tagcgtaagg cactacagcg
     1201 cattgcttta cgagtaataa caatggattt atcaccagaa gacgcaaagt ttttacgaga
     1261 actgcaacac cgtatgctca gagaagccat tatcgaacag caaacgggca tgagcgccat
     1321 agacccattg cgatacaaac gggcctcatc tcctttagtt gacgacaatt ccaatgcgga
     1381 taaaacacgt ccaccacact gattacattt ttcattagca cagtggtgtg atgcagcata
     1441 cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg gagcaactgt tacaggcacg
     1501 ccaacgggag tttgccacta aatggggacg tgccgcgctg ccacatgaac gcctaaccta
     1561 ttttccactt gaagacctca ttaaaaataa tggcaaaaaa atcaggtctg ataagaaaca
     1621 cttcttagaa tgcaatgaag acaaataaca acaatctgca tattgccacc aaccacagat
     1681 gtggcatcgt tatcagcgca cttgattttc tttatatcca ctgacgagct tgccggggca
     1741 cctttgtttg ccggtcattc tggtatcatg cagatattta cggcgtacaa gcccactagc
     1801 atgacattgt aataaggcct gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt
     1861 aatcgttgac aatatggtgc atgtaatcgt aagcgtccag cgaaatccat ctggtcataa
     1921 tctgaagcat ccgacaaagt ggtgtccacc aaataagtga tctgcgccca atatcctgag
     1981 ccggaacttt gacaaaggcc aacgaaaaat gggagcggat gttacagagt tctcggtaca
     2041 ggggaaaagt ggtacctgtc gccagttctc cgatctgttt aaccgggaaa ttatctccta
     2101 cagcttgtca gaaaggtcgg tgatggagca tcgttaatac gatgctatac gatgcattca
     2161 cagtgcccgg ccagaggatg ccccgtcgct gcatatggat cagggttggc aatatcgaat
     2221 cgcaggctat aggcaaagtt aaagccccat ggagtagcac aaaatatgcc gtacaaagga
     2281 aacggtctga ataacgcagt gatgaagaac ttcttcagca cactgctaaa acgcatagtg
     2341 atcgagcgct gattctggcg aacaactgaa ctaatacatc agaatctgca ttatgttaaa
     2401 taaatatgaa aagatggttt aaataccccg ttacttttga cttactctat acggtatcgc
     2461 atcgtttaat attcgcaccg gccagatttt tatttctatt agttgtcaca atactgaatg
     2521 cgtacgacca cagtattctg gctcctgtgt ggttatgctt taattctgcg ttccgggcag
     2581 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt tttaccagtg
     2641 ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat agcattgtcc
     2701 ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt ccgtttttca
     2761 taacccactg tactgataac cataatctaa tcagtagaaa ttgagtcgaa aataagcact
     2821 actccataca ggataattag aggtcagttt gattattcac aattcatcat cagcattttc
     2881 tatttctgac gaaatcaaca tgaaaataac catatatgat aattattata ataacggctt
     2941 taattggaat acatatatta caacgtatta tatataattg gtattctggg aactatattc
     3001 tcaaaataca gtagaaacga ggtatgtttc tggtggaaag gacagtggga ttaaaaagta
     3061 agaattgata aaaaaaacgc cagcaacaca tagtgccggc ggagggaata ccccatggag
     3121 aaaatgtgat gcctagagca tcatgaatat ataattaaaa atagttagcg ttgtcaatac
     3181 tttcacaaaa ataattttcg tagtatagaa agatattttt atgcatgacc tacctgaatt
     3241 tgctccgggt agaggttata aataaaaatt gaatcacgac aaacacaata ttcatagtat
     3301 ggcgatgcct acgccagcaa gaatagcgcc aataatattg ggaatataat agacaccaga
     3361 cgcacaggca tctcactcct taacaaacaa taatcaggat atttactttt acccagctaa
     3421 ctgtttacac ccaaagtgca cacaattaac cattcaataa caaatggcaa tatccatagc
     3481 catacgactt taccttgtaa tgttcggtat ttctttataa ttattctggg aaatctatca
     3541 t
//
LOCUS       DADLZM010000236         3517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLZM010000236 DADLZM010000000
VERSION     DADLZM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.241"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNY73_004015"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNY73_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY8049816.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNY73_004016"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNY73_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY8049817.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3517
                     /gene="cusC"
                     /locus_tag="JNY73_004017"
     CDS             2335..>3517
                     /gene="cusC"
                     /locus_tag="JNY73_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY8049818.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEKAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQ"
BASE COUNT          864 a          911 c          920 g          822 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaagcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc actaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaaa aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
     3241 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
     3301 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaattga gatccccatt
     3361 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
     3421 gtggtgaatt atgaacagaa aatccagaac gcctttaaag aggtggcaga tgcgctggca
     3481 ttacgtcaaa gcctggacga tcaaatcagc gcccagc
//
LOCUS       DADLZM010000237         3504 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLZM010000237 DADLZM010000000
VERSION     DADLZM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.242"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            29..1441
                     /gene="ospC1"
                     /locus_tag="JNY73_004018"
     CDS             29..1441
                     /gene="ospC1"
                     /locus_tag="JNY73_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY8049819.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHGSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(1545..1694)
                     /locus_tag="JNY73_004019"
                     /pseudo
     CDS             complement(1545..1694)
                     /locus_tag="JNY73_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1770..3467
                     /gene="ospD3"
                     /locus_tag="JNY73_004020"
     CDS             1770..3467
                     /gene="ospD3"
                     /locus_tag="JNY73_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY8049820.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLNPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1255 a          584 c          631 g         1034 t
ORIGIN      
        1 aaaactgttt tcatataagg ttcattttat gaatatatca gaaacactga actcagcaaa
       61 tacccaatgc aatatagatt ctatggataa cagattacat acattgtttc caaaagtgac
      121 atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa aagatagtgc
      181 aaatattatt aaagatttct ttaggaaaac tatagcagca cagagttata gtagaatgtt
      241 ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat cagacgctaa
      301 agcctcattt aaggctattg agcaccttga cagattatcg aagcattata tatctgaaat
      361 aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc taattattaa
      421 ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa ttttaaacat
      481 taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca catacgctga
      541 tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa tctctaacca
      601 tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg caaatgcgta
      661 tatcattgat catggctctc catatggata tatgacatta accgatcact ttgataatgc
      721 tattccacct gttttttacc atgagcacca atcattttta gataaatttt cagaggttaa
      781 taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac caatattcaa
      841 tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta gaaaaagtga
      901 agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg atctggatag
      961 aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa gtacagaaaa
     1021 cttcaaaaaa gttaagatta gagaaatatc tttagaggag gctgttacag catctaatta
     1081 cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc tttttctttc
     1141 gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg agataactag
     1201 acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac ttagcgctca
     1261 taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg atgtaaacac
     1321 aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa aatatgagaa
     1381 tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata aatatattta
     1441 atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg atatattgtt
     1501 tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt cgccccgttg
     1561 tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag cagcccccct
     1621 gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacgggg acaaaccaca
     1681 tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa gtctgcgtca
     1741 caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc ccatcaagga
     1801 aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca atccagtctc
     1861 tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta ttaaatctaa
     1921 attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta gcttcctact
     1981 ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt gcttttccgg
     2041 atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta
     2101 ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt ttcaatgaaa
     2161 tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat cacaatatgg
     2221 cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt cagctttatg
     2281 atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac ttagctaaaa
     2341 taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt tatggtctta
     2401 tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg tcctccataa
     2461 tcagatggcc taataattta cttaacccca aagttattta tcacgcgatg cgtatgggat
     2521 tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt tcagacaata
     2581 cgttagaatt acttttggca gccaaaaatg acgatggttt ttcaggattg cttttagctt
     2641 tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa acttctggac
     2701 ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga cgaatatcgg
     2761 gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac ggaaggcttc
     2821 tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg accgcttatt
     2881 attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa aatggacatg
     2941 cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc aactcagagg
     3001 atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt ctgttagcat
     3061 tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat gaggcaaaac
     3121 ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt ttatctggat
     3181 tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg aaaatacttc
     3241 acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa ggcccaaatg
     3301 gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata aaaacttata
     3361 tgggaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac ttggacaaaa
     3421 aaaatggaag tgattttcta gaaattatga agaatataaa aagctgaata ttatctcgtg
     3481 gtgatacact gaatttgagg taac
//
LOCUS       DADLZM010000238         3411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLZM010000238 DADLZM010000000
VERSION     DADLZM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.243"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            88..494
                     /locus_tag="JNY73_004021"
                     /pseudo
     CDS             88..494
                     /locus_tag="JNY73_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1078..1794
                     /locus_tag="JNY73_004022"
     CDS             1078..1794
                     /locus_tag="JNY73_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049821.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2346..2750
                     /locus_tag="JNY73_004023"
     CDS             2346..2750
                     /locus_tag="JNY73_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049822.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAIPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2813..3112
                     /locus_tag="JNY73_004024"
     CDS             2813..3112
                     /locus_tag="JNY73_004024"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8049823.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILQKAATYFAKRLK"
     gene            3109..>3411
                     /locus_tag="JNY73_004025"
     CDS             3109..>3411
                     /locus_tag="JNY73_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8049824.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASR"
BASE COUNT          953 a          705 c          795 g          958 t
ORIGIN      
        1 caaccgggag cgctttaagc tgggagtgat gttccactat gggaaactga atatggcgtt
       61 ctaagccttc ccaccatgat cggggaagac ccagatatga acaggcacat tcagagttac
      121 tttaaagagc ttactgaacg tctgaggtgt tagagccatg actgccgcag cattgctggc
      181 ggaaatgcct gaatcgagca gcctctcgag acgtgaaatc agcgcacttg tcggtgttgc
      241 tcaggtaaac cgggactcgg gaactctgcg cggtcgacga accattttcg gtgattgtgc
      301 tggggaagaa cagctctgta catggcggcg cttgaggccc ccggtttaat ctggtgataa
      361 aggcgtttta catgcgccta cttgcagccg gaaacgccaa aaaagtggca ctggttgcct
      421 gtatgcgtaa gttgctgacc attatgaacg caatgctcag aaaaaacgaa gaatggaatg
      481 aatcgtattt atgaaaactg gattgtgggc cataaatatt ccaaaaagat agcccgctcc
      541 gtttattacc ggaacgggcg agagcatgac attgtctgtt attataatga gactgctgat
      601 tctacttatt tttaatttca agaaaagcgt tttctacaaa gaattatgag atttaatgtt
      661 ttgtttagac aatattatca ttctgttcaa taatgaaagt cttcatcaac aacaagtaaa
      721 aacaacatcc ctctcatcga accggacttg cagatttccc gcatccggct ctcttgcaac
      781 acatcagacc ttcacgttcg ggcggttatg gggaaggtcc agcaagctgt atgagcccta
      841 actgtttgaa taccagtaga ttaccagaaa cttctatcga ttgaagacat taccaactag
      901 cgttactaaa tgttgaccaa gttttgttca cttgtctggt tttgatagta gcattctgca
      961 gagtattttg atatgataaa tatgcattaa caatgatatt attgttaatt tcctagcata
     1021 aaaattaaaa gatcaacttt tatctcgaat attttttaca ttatcagttg gtttcttatg
     1081 aatgacaatt cattgcttag aaattcttca ttattcatcg cctatatggg atgtgtaggg
     1141 tgggtcagtg cttattctta tggctgggga acctcttttt actatggatt tccatggtgg
     1201 gttgttggtg caggtttgga tgatgttgcc agaagtttac tctatgctat catagtaatg
     1261 ggtatacttt ttaccggttg ggggattggt gttttatttt ttcttcttat aaagaaaaga
     1321 agtaaaatac aagacttgag tttttttaga ctattttttg ctattaccct actgtttttc
     1381 cctgtaatat tcgaactttt aattctcaaa caatatttca tacttccatt atcactttca
     1441 tgcattatta gttcattggt tatttcaata ataattcgta tatatggacg gatcttttct
     1501 gtatcatgtt tttcagatat cccttttgtt agaaaacacc gcattaagtt aattatggct
     1561 ggttttttgg tttatttttg gcttttttcc tttcttgttg gctggtataa acctcaatta
     1621 aaaaaagaat atcagatgct ttgttataat aatagttggt attatgttct tgctcgttat
     1681 gattcacggc tcgttttgtc tagttcattt aaagatgaca gtaacagatt cttaatattt
     1741 aatacagaac agtcgggatt ttatgaaatc aatgatgttt atgtcagaaa gtaataatcc
     1801 tctcccacaa agtgaattaa atgaatatat aatgcgttcg aagtatatgt attgagctgt
     1861 catactgaga aacaaacctt agggcagcac aattaaagcc agaaacaact atgcaagaaa
     1921 gttactaaat aagtgctcgg aacagtaaaa cactcagggg ggactcagtc cggattatgt
     1981 aatcagataa attgccaaac atcacaaatc accagccgga ctgagtgatg ccaagcattg
     2041 caccaatacc ccgtgatgaa cgatgcctga tgcagaaagc tccctataaa acaaagaaca
     2101 aaaactacga ccggcgattc accgtcatac tgatgctgca tcgtggtgac actgtctgtc
     2161 actttcatgt tgactgaaca actctgctgc gaagaaggtg taccgccggc gggatttgat
     2221 tgtccggcgt ctagcttcca cttccctgat ggaactgaaa gttgttatgg ccggtcagct
     2281 tgccctgtcc gcaggacatg aggtgctgtg acggacagca ccagtaacgg aaagtgacaa
     2341 tgaaaatgaa tactgcgctg atagttgcac taatgtgtat gtggtatgcg gtacccgctg
     2401 ctgcaaaaga gactttactg gctataccac gtaattcaac agaacattgc tatgcggaga
     2461 ttaatgtgca cggaccatat ggggtgtatt tccgagtggt cccgcatccc cccgggggaa
     2521 agagctgggt cgaatgtaac agtgactact attactctga taaaccaccg ggagtacaga
     2581 tactcggaac ccgtgcaggg tgtcgggtat acggaatctg cgggaccacc tcgacactgc
     2641 atgtggctgg aagaggggtt gtgtgtatta aaaatatatg cagtccacgt gggatgataa
     2701 ttcacaggat acggaaacgt cctgtagtgg ctgtaagcga tgagatgtga tcttacccag
     2761 caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc acatgacaaa
     2821 aacagtatca accagcaaaa aaacacgtaa acagcattcg cctgaatttt gcagtgaagc
     2881 cctgaagctt gctgaacgca tcggtgttgc tgccgcagcc cgtgaactca gcctgtatga
     2941 atctcagctc tatgcgtggc gcagcaaact gcagcaacag atgacctcgt ctgagcgtga
     3001 aagtgaactg gccgctgaaa atgcccgcct gaagcggcaa ctggcggagc aggcagagga
     3061 actggctatc ctccaaaagg ccgcgacata ctttgcgaag cgcctgaaat gaagtatgtc
     3121 tttattgaaa aacatcaggc tgagttcagc atcaaggcaa tgtgccgcgt gctccgggtg
     3181 gcccgcagcg gctggtatac gtggtgtcag cggcggacag gaataagccc tcgtcagcag
     3241 ttccgtcagc actgcgacag cgttgtcctc gctgcggcct ttacccggtc aaaacagcgt
     3301 tacggtgcac cacgcctgac ggatgaactg cgtgctcagg gttaccactt taacgtaaaa
     3361 accgtggcgg caagcctgcg ccgtcagggg ctgagggcaa aggcctcccg g
//
LOCUS       DADLZM010000239         3388 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLZM010000239 DADLZM010000000
VERSION     DADLZM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.244"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(185..880)
                     /locus_tag="JNY73_004026"
     CDS             complement(185..880)
                     /locus_tag="JNY73_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY8049825.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(873..2300)
                     /locus_tag="JNY73_004027"
     CDS             complement(873..2300)
                     /locus_tag="JNY73_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY8049826.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2311..3030)
                     /locus_tag="JNY73_004028"
     CDS             complement(2311..3030)
                     /locus_tag="JNY73_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY8049827.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          847 a          945 c          708 g          888 t
ORIGIN      
        1 ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg taaatatcct ggcattacta
       61 atgcctggat atttacagaa gagcagcata accatgacgg aacggggtta agtcaggttg
      121 tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt ttatgttcag atctcatatc
      181 tctctcagca atcctcaata atcagataca ccgctttcac cggaccatga accccgacga
      241 ctttgataag ctcaatatcc gccgttgagc tgggaccact gatgatgtta atacatgacg
      301 gcattcgttc accggcctgc gctttctgat gcaatttttc tgcgagttgc gctacacgcg
      361 gcaggattgt gcttttacgc aggataaaaa gagaagattc cgggagcagg ctcaatgaac
      421 gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa ccatattcag
      481 catacacgac acccacttta gcctgctctg cctgcgagat attctcggca cctttcgccg
      541 gatcccaaac aacggcattg cattcctgct gcaaacgttc actaatcccc aattcctcca
      601 gcctcgtgtc accgctaatc acgaccgact gatctcccag ctctttacac agacgtattg
      661 cagcttctgc cgccttcgcc tcgctggtca gctcacagcg cgtcaacata acatcgctgg
      721 caaactgaat aaacgcgtca cagcgctgct gttggttaag ttgggtaagc cgctcgttag
      781 catagttgtt aagcggcgca tcttctgctt gcggttcaag tcgcagcggg cgacccagtg
      841 cctgagcaac gttattcaaa aattcgctcc gattatccat tctttttctc ctgcgcctga
      901 tgtttcttaa accaactacg gaaactctct ccgtcagctt caggaagatc gcgtgcttcc
      961 atccagtcgc taatcgcgcc aaatttgagt ggtgttttac cgccattgat aaaccagctt
     1021 gccgcatgag cgccggccat catcccgact ttccacaatc ctggatgact attggcataa
     1081 gcgaacattt ttatcgcccg ttgctctgct tttgcggtga tccctttttc agccatcacc
     1141 cgacgatgac gcaaaatcag ttttgacagc ggaatacgca ccggacacac gctgtcacaa
     1201 gcagtgcata aagagcaggc gtagggtaaa tctttaaaat ctttatagcc gccaagtagc
     1261 ggagaaatca ccgcaccaat tggccctgga taaatagagc catatccatg accgccaata
     1321 tggcgatatg ccggacaagt attcatacaa gccccgcagc gaatacagcg cagcacatcc
     1381 cgaaattcag aggccagcac ctcagaacgc ccgttatcga caataaccag atgaaactct
     1441 tcaggaccat caacgtgccc cgcttcgcgc ggccctgtca gccaggtgtt gtatcccgtc
     1501 aaacgtgcac caacggcact gcgcgccagc atggtgatca atacatctac ctcggcaaac
     1561 gtgggggcaa tacgctccat tcccatcact gcaatatgcg ttttaggcag cgtggtacac
     1621 attcgcgcat taccttcatt ggtcaccagg cataccgaac cggtctctgc caccgcgaaa
     1681 ttacagccgg taatacctat ttcagcactg aggaaatctt cacggatttt ttgccggatg
     1741 aataaggtca tcgcttcagg cgtttccggc ccctcatagc ccagacgttc gtgtagcact
     1801 cgacggatct gatggcgatc tttatgaatt gccgggacca caacatgaga tggcggatct
     1861 tgatccagct ggagaatata ttcacccaga tcggtttcaa tcacctgaat gccagcatcc
     1921 tgcaacacat gattgacacc aatctcttcg gtcaccatcg atttagattt caccaccttc
     1981 cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc tttggttttt
     2041 gcaaaataga cgtgaccgcc gttttgcgtc actttttctg agagctggta cagataagcg
     2101 tcgagattac tcaaaacatg atcacgtatc tgggcggccc gatcgcgcca ctcctcccag
     2161 tgccccaatt catcgaccat tttttgccga tttgccccaa tacgctgctg cgcgtttgcc
     2221 accgctttgc gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta
     2281 ttactggttt tgatcgacat ctttatatcc tcagcggctc atcaacactt cagcaatatg
     2341 catcactttg actttctgcc cttcccgttg taatcgccca ctgatgttca gcaggcaact
     2401 cacgtcagca ccaattaaat actcagggcg gacttccatc aggtgcacaa ccttttcttt
     2461 caccatctcg ccggatattt cggccatttt gaccgagaac gtgccgccaa atccgcagca
     2521 ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc acatttttca gcagcgtaag
     2581 tggctcgtcc ttcactccca gcttacgggc caggctacaa gatgggtgat acacagctct
     2641 cccttgcaaa ctggcaccta catcgactac ccctaattta ttaacaataa aagaggtgag
     2701 atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt catccgccag
     2761 atacgtcggg taacttttta cggcataggt gcaagagcca gccggtgaaa taatgggatc
     2821 gtcgttatcc tccagtgcgg cgatcagatt tttcatccct ggaatcgctt ctttgatata
     2881 accgctattg atcgcaggct gaccgcagca tccctgtttc tccgggaaat ttacgcgaca
     2941 gccgagtttt tccagtagca gcacggagtc tcgtgccatt cttgatttca gggcgtcacc
     3001 aatacaggtg acaaagaaat tgacattcac aactaatact ccattacttc atgcccattt
     3061 atgcccgatg tttccccccc accgggggat caatcatcaa taaatatggc gttcttttta
     3121 aattggctag caacatacca cccctcattt gtatgacaaa tgataatctt tcatccaaac
     3181 ttcaaatgca ataactaatt ttatttcttt cggcatcgct cacattttat ggggaaatcg
     3241 acgatcattt ttggcataag aaatccattt aaaaatggct acatccctta tacctattca
     3301 gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaaa aatcaccgac
     3361 aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DADLZM010000240         3278 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.245, whole genome shotgun sequence.
ACCESSION   DADLZM010000240 DADLZM010000000
VERSION     DADLZM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.245"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(255..560)
                     /locus_tag="JNY73_004029"
     CDS             complement(255..560)
                     /locus_tag="JNY73_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY8049828.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            668..1378
                     /locus_tag="JNY73_004030"
     CDS             668..1378
                     /locus_tag="JNY73_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY8049829.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(1381..1910)
                     /gene="speG"
                     /locus_tag="JNY73_004031"
                     /pseudo
     CDS             complement(1381..1910)
                     /gene="speG"
                     /locus_tag="JNY73_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1976..2317)
                     /locus_tag="JNY73_004032"
     CDS             complement(1976..2317)
                     /locus_tag="JNY73_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY8049830.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2359..>3278
                     /gene="rspA"
                     /locus_tag="JNY73_004033"
     CDS             2359..>3278
                     /gene="rspA"
                     /locus_tag="JNY73_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY8049831.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
BASE COUNT          842 a          741 c          814 g          881 t
ORIGIN      
        1 gttgatttaa cgagtgttct gcggctgatg cccaccattc ggtcattttt ggacataact
       61 cactccctgt tctttatcgt tatataaatg tttatatatt gaatatttag cgcgctaaca
      121 atagagggag tctacccatt ttgggttaag aattattaat ccacatcaat agaagggtat
      181 gagtaataag gtgagattat gttgtatgtt caaatcgccg gatgtgtcat atccggcgtt
      241 cagtcgataa tgtgttactg cggttcggca ggcgcgccat cctgggtaga ctgcgcggga
      301 gcagagacgt taccgctggt ggtgcggttg tagagaattt tatgcgtatc attagcgcaa
      361 tgcccgacga cctgggaatc aggctgatca acctggtcat tgggtacaat acttaacgtg
      421 aagctgcttt cgggtacgcc attattgata atgcgctgtg atatatcgct ctgtatgcgc
      481 tcacaggatc ccggcgcggc gagtaccgcg ggtgaggcga gggcgagcag aagcgcggca
      541 cagcaggttg agagtttcat cataagctcc ttacgcgaag ataacttctt taagcatagc
      601 atttaacgtg taaagtactg tatttgctac tatgattgag aatcatctct actctctggt
      661 gactgttgtg aaatacaaat tactaccatg cttactcgcg atattcctca caggatgtga
      721 ccgcacagag gtaacacttt catttacccc tgagatggcc agtttctcta atgaattcga
      781 ttttgatccg ctgcgtggtc cggtaaaaga tttcactcag acattaatgg atgagcaagg
      841 tgaagtgacg aaacgtgttt ctgggacttt gtcggaagaa ggctgttttg attcactcga
      901 attactggat ctggaaaata ataccgtggt cgctctggta ctggacgcca attattaccg
      961 tgatgccgag acgctggaga agagagtacg tttacaggga aaatgccagc tagcagaatt
     1021 accttctgcc ggggtgagtt gggaaaccga tgataatggc ttcgtgatta aagccagcag
     1081 caaacaaatg cagatggaat atcgctatga tgatcagggg tatccgctgg gtaaaaccac
     1141 gaaaagtaac gacaaaacat tatctgtcag cgccacgcca tcaacggatc cgatcaaaaa
     1201 attagattac acagcggtta ctttactaaa taatcaacga gttggtaatg taaaacagag
     1261 ctgtgaatat gacagtcacg ccaatccggt ggactgtcag ctgatcattg ttgatgaagg
     1321 agtaaaaccc gccgtcgaac gggtttacac catcaaaaat acgatcgatt attattaatg
     1381 ctattgtgcg gtcggcttca ggagagtctg acccggtgtt ttgtgctctg ccagatactg
     1441 atgctggaat atacacatgc gaatggcatt acgatattga ccattaataa aaaactcatg
     1501 catcaattca ccttcaaccg taaagccaag cttgcggtaa atgtgaatcg ctttttcatt
     1561 ctctttatcg acgatcagat acagcttata gagattgaga acggtgaagc catagtccat
     1621 tgctaatttg gcggcacggg ttgccagacc tttcccctga tactccgggg agataattat
     1681 ctgaaattct gcccggcgat gaacatggtt aatttccacc agctccacca gaccggcttt
     1741 ttcgccgtca cattccacca caaagcgccg ttcgctctga tcgtgaatat gcgcttatca
     1801 tacagatcag agagttcaac aaaggcttcg tagggttcct caaaccagta acgcatcaca
     1861 ctggcgttat tgtcgagttg atgtacatag cgtaaatctt cacgctccag cgggcgtagc
     1921 ttaactgtgg gcgcttggca taacgtgtcc ttacattcct taaatcaata acaggttagg
     1981 gggtaataac gcggccagtt cgacggtcca ggcagcgcaa agtattgggc tcccagtagg
     2041 cattgatgtt ggcgctttgc tcacatttat cgcggttatc aaaagcggcg tcggctttat
     2101 cccactcttt ttcagtgcgt ttattcactt tctggcgcag attgcgcgtg tcattccatt
     2161 gctctttttc catagcggcg tgctggcggc tttgtgcact gtcgccagac tcaatcacca
     2221 gtttgttagt ttcggcatga acagttgtgc tcaatgccag tgcgcaaggc agcagaaaag
     2281 cgagcaggcc gattcgtttg ctgagagtga ttttcataat tcattccctg tatgaatgat
     2341 taaaggtgat tctacaccat ccactgcgaa cgcaaaacgt accaggctga agtttttgtt
     2401 acctgtccgg ggcgtaattt cgtcacatta aaaatcacca ctgaggacgg tattacgggc
     2461 cttggggatg ccaccctcaa tggacgtgag ctttccgtgg cctcttattt gcaggatcat
     2521 ctttgtccgc ggcttattgg tcgcgatgcg caccgtatcg aagatatctg gcagtttttc
     2581 tataaaggtg cttactggcg tcgcggtccg gttacgatgt cggccatttc agcggttgat
     2641 atggcgctgt gggatattaa agccaaagct gccaacatgc cgctttacca gttactcggc
     2701 ggcgcgtctc gtgaaggggt gatggtttat tgccatacca ccggtcacag tattgatgaa
     2761 gctctggatg attatgcccg tcatcaggag ctgggattca aagccatccg cgtgcagtgc
     2821 ggaatccctg gtatgaaaac cacctacggc atgtcgaaag gtaaaggtct ggcttatgaa
     2881 cccgcaacca aaggacagtg gccggaagag cagctgtggt cgacggagaa atacctcgat
     2941 ttcatgccga aattgtttga cgcggtacgt aacaagtttg gttttaatga acatttgctg
     3001 catgacatgc accatcgctt aacgcctatt gaagcggcgc gctttggtaa aagcattgaa
     3061 gattatcgca tgttctggat ggaagacccg acgccagcgg aaaaccagga atgcttccgt
     3121 ctcattcgcc aacataccgt cacacccatc gcagtgggtg aagtcttcaa cagcatctgg
     3181 gactgcaaac aactgattga agagcaactc atcgattata tccgcaccac gctgacccat
     3241 gcaggcggaa ttaccggtat gcgccggatt gccgattt
//
LOCUS       DADLZM010000241         3257 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLZM010000241 DADLZM010000000
VERSION     DADLZM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.246"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..205)
                     /gene="mokC"
                     /locus_tag="JNY73_004034"
     CDS             complement(<1..205)
                     /gene="mokC"
                     /locus_tag="JNY73_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY8049832.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYES"
     gene            734..1900
                     /gene="nhaA"
                     /locus_tag="JNY73_004035"
     CDS             734..1900
                     /gene="nhaA"
                     /locus_tag="JNY73_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY8049833.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1966..2865
                     /gene="nhaR"
                     /locus_tag="JNY73_004036"
     CDS             1966..2865
                     /gene="nhaR"
                     /locus_tag="JNY73_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY8049834.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2903..>3257)
                     /locus_tag="JNY73_004037"
     CDS             complement(2903..>3257)
                     /locus_tag="JNY73_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049835.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          765 a          736 c          795 g          961 t
ORIGIN      
        1 cggattcgta agccgtgaaa acagcaacct ccgtctggcc agttcggatg tgaacctcac
       61 agaggtcttt tctcgtcacc agcgccgcca ctacggcggt gatacagatg acgatcaggg
      121 cgacaatcat cgccttatgc tgcttcattg ctctcttctc cttgacctta cggtcagtaa
      181 gaggcactct acatgtgttc agcatatagg aggcctcggg ttgatggtaa aatatcactc
      241 ggggcttttc tctatctgcc gttcagctaa tgcctgagac agacagcctc aagcacccgc
      301 cgctattata tcgctctctt taacccattt tgttttatcg attctaatcc tgaagacgcc
      361 tcgcattttt gtggcgtaat tttttaatga tttaattatt taactttaat ttatctcttc
      421 atcgcaatta ttgacgacaa gctggattat ttttgaaata ttggcctaac aagcatcgcc
      481 gactgacaac aaattaatta ttacttttcc taattaatcc ctcaggaatc ctcaccttaa
      541 gctatgatta tctgggctta gggtcactcg tgagcgctta cagccgtcaa aaacgcatct
      601 caccgctgat ggcgcaaatt cttcaatagc tcgtaaaaaa cgaattattc ctacactata
      661 atctgatttt aacgatgatt cgtgcggggt aaaatagtaa aaacgatcta ttcacctgaa
      721 agagaaataa aaagtgaaac atctgcatcg attctttagc agtgatgcct cgggaggcat
      781 tatacttatc attgccgcta tcctggcgat gattatggcc aacagcggcg caaccagtgg
      841 atggtatcac gactttctgg agacgccggt tcagctccgg gttggttcac tcgaaatcaa
      901 caaaaacatg ctgttatgga taaatgacgc gctgatggcg gtatttttcc tgttagtcgg
      961 tctggaagtt aaacgtgaac tgatgcaagg atcgctagcc agcttacgcc aggccgcatt
     1021 tccagttatc gccgctattg gtgggatgat tgtgccggca ttactctatc tgccttttaa
     1081 ctatgccgat ccgattaccc gcgaagggtg ggcgatcccg gcggctactg acattgcttt
     1141 tgcacttggt gtactggcgc tgttgggaag tcgtgttccg ttagcgctga agatcttttt
     1201 gatggctctg gctattatcg acgatcttgg ggccatcatt atcatcgcat tgttctacac
     1261 taatgactta tcgatggcct ctcttggcgt cgcggctgta gcaattgcgg tactcgcggt
     1321 attgaatctg tgtggtgtac gccgcacggg cgtctatatt cttgttggcg tggtgttgtg
     1381 gactgcggtg ttgaaatcgg gggttcacgc aactctggcg ggggtaattg tcggcttctt
     1441 tattcctttg aaagagaagc atgggcgttc tccagcgaag cgactggagc atgtgttgca
     1501 cccgtgggtg gcgtatctga ttttgccgct gtttgcattt tctaatgctg gcgtttcact
     1561 gcaaggcgtc acgctggatg gcttgacctc cattctgcca ttggggatca tcgctggttt
     1621 gctgattggc aaaccgctgg ggattagtct gttctgctgg ttggcgctgc gtttgaaact
     1681 ggcgcatctg cctgagggaa cgacttatca gcaaattatg gtggtgggga tcctgtgcgg
     1741 tatcggtttt actatgtcta tctttattgc cagcctggcc tttggtagcg tagatccaga
     1801 actgattaac tgggcgaaac tcggtatcct ggtcggttct atctcttcgg cggtaattgg
     1861 atacagctgg ttacgcgttc gtttgcgtcc atcagtttga caggacggtt taccggggag
     1921 ccataaacgg ctcccttttc attgttatca gggagagaaa tgagcatgtc tcatatcaat
     1981 tacaaccact tgtattactt ctggcatgtc tataaagaag gttccgtggt tggcgcagcg
     2041 gaggcgcttt atttaactcc acaaaccatt accggacaga ttcgagcgct ggaagagcgc
     2101 ctgcaaggca aattatttaa acgcaaggga cgtggtctcg aacccagcga gctgggagaa
     2161 ctggtctatc gctatgccga taaaatgttc accttaagcc aggaaatgct ggatattgtg
     2221 aactatcgca aagaatccaa tttattgttt gacgttggcg tggctgatgc actttccaaa
     2281 cgcctggtca gtagcgtact taacgccgca gtggtagaag gcgagcccat tcatcttcgc
     2341 tgcttcgaat ccacccacga aatgctgctg gagcaattaa gtcagcataa actggatatg
     2401 atcatttctg actgtccgat agactctacg cagcaggaag gcctgttctc cgtgagaatt
     2461 ggcgaatgtg gcgtgagttt ctggtgtaca aatccaccac cagaaaaacc gttcccggct
     2521 tgtctggaag aacggcgact tttgattcct gggcgacgtt caatgttagg gcgcaaattg
     2581 cttaactggt ttaactccca gggattaaac gtagaaatcc tcggcgagtt tgatgatgcc
     2641 gctttgatga aagcttttgg tgcgatgcac aatgcaatct tcgttgcccc aacgctttat
     2701 gcatatgatt tttatgccga taaaactgtc gtagaaattg gtcgcgtcga gaatgtgatg
     2761 gaagagtacc atgctatttt tgctgagcgg atgattcagc acccggcggt acagcgaatc
     2821 tgcaatacgg attattctgc gctttttagt ccagcggtgc gttaatcggc agctccccca
     2881 aagttaaggt gggggagata gattagttgt acattaccac gattttgact cggctcatta
     2941 tttgcccgct tgagacattg tttccatatg tacgcgggcg aataaataga ggaatctgat
     3001 tacttccttc atggggatgc tgaaaagagt agtaattgct tttaccatac tggatttcat
     3061 tcgtaccatc attggagagt tgaaagccta tttgacccgc accgtcctca ctgttttcta
     3121 actcattttt taatatgctg gttcctgact cctgtatcgt cactggttca aagtaatatt
     3181 caacgtcact gggttcaaaa actaattcac cttgctcgcc ctggatgcag tcaaatctta
     3241 tatgatgaaa ctgataa
//
LOCUS       DADLZM010000242         3239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLZM010000242 DADLZM010000000
VERSION     DADLZM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.247"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..328)
                     /locus_tag="JNY73_004038"
     CDS             complement(<1..328)
                     /locus_tag="JNY73_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8049836.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENI"
     gene            574..1335
                     /locus_tag="JNY73_004039"
     CDS             574..1335
                     /locus_tag="JNY73_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8049837.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(1442..1954)
                     /locus_tag="JNY73_004040"
     CDS             complement(1442..1954)
                     /locus_tag="JNY73_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049838.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(2093..3239)
                     /locus_tag="JNY73_004041"
                     /pseudo
     CDS             complement(2093..3239)
                     /locus_tag="JNY73_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          973 a          725 c          579 g          962 t
ORIGIN      
        1 ttatgttttc actgatgtaa aagcctactt tgggtatcgt aagaatcaca ttttcttcaa
       61 caccaatatt tttaaaactt ttgcgtaaca gggatatata ttggttcaat gtgttgtttg
      121 acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg tcaaccgctc
      181 caccattact ttccagtaac actgccaaaa tacaccccgc cgtagctggc aaatttatct
      241 catcctgggt atttacattc gagagcgagc atttgttcgg atcaaaaata atcgcgtcat
      301 tgataatgaa aaaacatctc cgctccatgc aggcaacaac acaaaataag attttgtcat
      361 tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata accctgaaaa
      421 agtcatataa aacttatatc cacagaataa aaaaccaaac atgtcagttt tatcaatata
      481 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
      541 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
      601 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
      661 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
      721 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
      781 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
      841 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
      901 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
      961 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
     1021 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
     1081 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
     1141 gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
     1201 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
     1261 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
     1321 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
     1381 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
     1441 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
     1501 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
     1561 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
     1621 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
     1681 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
     1741 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
     1801 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
     1861 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
     1921 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
     1981 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
     2041 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
     2101 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
     2161 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
     2221 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
     2281 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
     2341 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
     2401 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
     2461 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
     2521 tactcagtcc cggctagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
     2581 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
     2641 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
     2701 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
     2761 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
     2821 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
     2881 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttccgat
     2941 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgcta
     3001 tgcctctcca taccgtacta taactcagtc tggtagaccg cgtgtttcct gtgccccggg
     3061 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
     3121 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
     3181 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagac
//
LOCUS       DADLZM010000243         3205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLZM010000243 DADLZM010000000
VERSION     DADLZM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.248"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(419..616)
                     /locus_tag="JNY73_004042"
     CDS             complement(419..616)
                     /locus_tag="JNY73_004042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049839.1"
                     /translation="MLGDNPNIDVVAMVPIIIVDMKIEVTLKLSDITVGNIMSAVSIM
                     AKDTLNTESVEKRLLSEKCMA"
     gene            1462..2511
                     /locus_tag="JNY73_004043"
     CDS             1462..2511
                     /locus_tag="JNY73_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY8049840.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
BASE COUNT         1040 a          561 c          538 g         1066 t
ORIGIN      
        1 attttttatt ttttttatca tcattcaatg aacagatagc tgtagtcaat gtattattat
       61 gcaccacact gttaattttt tgcaaaacaa acaattacaa tacaaaacca ttattaaatg
      121 cgatggggat ttcactatta gttattattt atattgcaac ttgcacagga aataaagtca
      181 ggtattatgc agagatagaa cattggttta aagagtatgg taattttaat ataatccaaa
      241 gatctatgct tggacttaac ttatatgcag atatgttatt ttctgtaaaa tcaattatcc
      301 cagcattact agctttttcc agtactatta tatgcaagaa aaaaacaaaa atactccctc
      361 taatttcttg ctgtatcttg atcacactat atattataca tacaccgcca gttatttttc
      421 aagccataca tttttctgaa agcaatcttt tctcgacact ctccgtgttt agggtgtctt
      481 ttgccatgat acttacagca ctcattatat ttccaaccgt aatatcactt aattttaatg
      541 tcacttcaat tttcatatcc acaatgatta ttggaaccat tgccacaaca tctatgttag
      601 gattatcacc aagcatatat gcttctggta atagaattta ttttattcca tatttgcttc
      661 ttataacagc aattgttgtt tcaaccccaa tcgcaataaa taacatagta gcaaattttg
      721 ccaagagcca ttataaaatt tgaattaaat ttaaaagcca cctccttttt ttgggggggg
      781 cttagaaata aaaacaaatt taattggact tttattggtt ataacacatt cctctatact
      841 gctcatcacc cgactatact aggattttcg tcattttttg ctgaaccgct ctcatgaaat
      901 cctgacaaca aagtcatgtt tcacgtcagg atacttcttc tggcatggaa atataccgtg
      961 tgcagctgat gcatatttat atttcaatat aatacgatat tgaatggcct acaccactgg
     1021 tagttcaggc caaatgacat ccgacggttt cacgatgaac tacggcaaaa gaatatcaga
     1081 gccctcattc ctccccgaaa gggagcgggc tctggtctgg tgagtatgca gatcgtaaag
     1141 gctatggcca tggtgcgaga gtggcagcta tgccagaaag agtgtgtatt gcctgagact
     1201 ctaacctgat gcagagacga ttgtcttgaa cctgatttat tcaacaaagc cgctggcagg
     1261 atgttacgaa agttatcgcg gccgcaggct gatttgatat cgatactatc ggtggtactg
     1321 cggaagtaaa tgcctatata gtgcgacttt gaaaaataat aaattaatat tgaactcgtt
     1381 gattaataat cataaacttt caggcattca tatttgcaat gagtgtttga aaatcacaca
     1441 aaactggaga gcctacttta aatgtataat aacaaaatcg acaacaacag aaaaacaagg
     1501 catgattggg ttgatgcttt aaaatttctc ggtatttttg ctatatattt aggtcacctt
     1561 ggtttggggg ctggcaaatt gtatcctttt gtttttagtt atcacgtccc actattcttt
     1621 ttcgctgctg gtttttttac tatcaaaaaa aatgacttat ctattttcga ttatatcaag
     1681 tcaaagtttt acagattaat gattccatat ttcacttttg catttactat attaataata
     1741 aacacaatta actcaggtga gacgattgat tatatatata gccacattta tgatataata
     1801 tatggtgtta gaaacaacca atttgtagga acaatatggt ttataaactg cctttttgtt
     1861 ataatagcaa ttgatgcaat ctttaaagaa atagtcaaga acaatatagt gattttaatc
     1921 atatcactat tatcgttcat gttgtcacaa acagtgctca atcataaccc attactagat
     1981 cctcaatggt tttggaacat agactccgct atggcatact ggtggttatt gcctctcggt
     2041 agatgtatgt ttttagaatt aacacgtgac aggttttttg gaaaatctaa aattggtttc
     2101 attgtcttct caattaccgc gataatgagt gcgtatcaac tccttaacca gaaacctttg
     2161 ctttttaaga ttatctcaat attcaatgca gacataatat caagtagtta tattcaagct
     2221 attaatacta ttatcaccac ggtaggtttg ataatattta atatttttat agcaaaaata
     2281 atttgtggga atgactttat tgtaagagct ggaagaaata ctcttaacat atgtggaatg
     2341 gagtatatca ccaaattatt tatccctatg gctttagcaa tcataggctt cagcgtgacc
     2401 attccaaatc ctatctgcgc aatcatatat acctgtattt gtgtatatgt atcagataaa
     2461 attggccatt ggctatcgag gacagtcggt gggccatttt tgataaaatg ataaaaaggc
     2521 gcaattaatt gcgccttttt atagatatat agcgcatatt tatgctcaga ttatactgtt
     2581 aaaattaatg cgcaaatgtt acggttttct ttcctagcaa ctgaagggaa tgttcgaagt
     2641 gcaaacataa aattacccct tcactgaaca gatattcagt cggccaacac atgagaaaga
     2701 aatacaccag atgaattaaa atatcatata cttattaatc aaagaaataa aaacataaac
     2761 agtaggtggt gacgctgata aactggctat aaacaacctg gaaggtgatg agctatcgca
     2821 atataattta ttggtgaatt atttgaagcc gattgataac accagtgctc aaaagactcc
     2881 ccccggtaac cataagtagc taatatctag tacagtttcg atatctatta tcgttactgc
     2941 gtcgatatag tctaaatagc tgcacctaat tctgtcacac ttgtcgttta cagatgggta
     3001 atgtgttgcg tatgggcaac aaaactcaag ccagatataa cacaacctag ctgagaacgc
     3061 cttcctgatg cttcgcccag caagggctga gcgaaagtgg agcacttttc tgcgcagcta
     3121 tttaggacag tattgagtca tattgtttat gtatgagtta agtttcgtaa tatcaattaa
     3181 ataaaataag ttgaaatcat tgtag
//
LOCUS       DADLZM010000244         3144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLZM010000244 DADLZM010000000
VERSION     DADLZM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.249"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(36..146)
                     /locus_tag="JNY73_004044"
                     /pseudo
     CDS             complement(36..146)
                     /locus_tag="JNY73_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(470..1147)
                     /gene="ospD1"
                     /locus_tag="JNY73_004045"
     CDS             complement(470..1147)
                     /gene="ospD1"
                     /locus_tag="JNY73_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY8049841.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNSHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(1518..1655)
                     /locus_tag="JNY73_004046"
                     /pseudo
     CDS             complement(1518..1655)
                     /locus_tag="JNY73_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1737..2035)
                     /locus_tag="JNY73_004047"
                     /pseudo
     CDS             complement(1737..2035)
                     /locus_tag="JNY73_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2624..2783)
                     /locus_tag="JNY73_004048"
                     /pseudo
     CDS             complement(2624..2783)
                     /locus_tag="JNY73_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2787..3144)
                     /locus_tag="JNY73_004049"
                     /pseudo
     CDS             complement(2787..3144)
                     /locus_tag="JNY73_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          836 a          675 c          597 g         1036 t
ORIGIN      
        1 aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg gttttcaaat
       61 tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta atcgcacttg
      121 atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg gctttatgca
      181 ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc ctgtatttcc
      241 cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta catttattgt
      301 gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg ttctatgggt
      361 agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag atcacacaat
      421 tcggggagag atccttctaa gtgcaattct ttgcagctat ttagcacact taaaacaact
      481 cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta agtctgtctg
      541 ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc atcatagcct
      601 cgatattccc tttccctgcg gccagaaata atcctggtaa cccctcatta tctcttacat
      661 ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa tctccataag
      721 cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat tccccagcat
      781 tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct gccaacagcg
      841 attcaccgta tttacgcgct gcatcggcat ttccatgcca aatcaatgaa aacaaattat
      901 ggctgttata ctccttatta ttctttgtcg gaatggatat gctctgtatt ttggccaatt
      961 ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg gcgtgggata
     1021 tagcggtatt tgtcacgtta atgatattat ttttcattcc tttattttca ttagcagtat
     1081 tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca tagttattta
     1141 ttgacatttt tgttcatcct ttttgctaaa aaaaacaata acctattttt gtaatgtaat
     1201 agatattagt ctattgctaa tctaaaaaaa ccattgccac attgaatggc tatattcacc
     1261 aataaattct ttcgatttcg tatattgaca tatcgtagat atatcttatc cctaagattc
     1321 aataatcgag tccgtttacc attctgtgca agcgccttca ctcagaagtg accgtcaagg
     1381 cgatcaccaa acttgataat aaagcagctc attgccatac gccagtcctt aaggggcgtt
     1441 gtccatttct gtgatgctgc ctagatttaa ctgccagctc cggcttatct acatctaaat
     1501 atgtgagcct aataccgcta cactttgtcc agactatgtt tttctccggg aagtgggcta
     1561 accgtctcca taaagtgacg aacttttttc aacagtggcc atattgaacg gcactgatga
     1621 ttacgggtta ttgtctcatg aagagccggc cacatcactg acagcagtga cttcaggcct
     1681 tcagcaccag acagcctgaa ccagttaatc catcgcccga cagatttagt aatctagggc
     1741 tggtttttct tcagctgttg cggttcgaaa gtgccattac gatcacgcag agtacggatt
     1801 tccagaggtt catctccagt agtgataatc ttcgttaagc aaccattgcg ggaattgctg
     1861 ccagatctaa gctgattttt ctcgcaccca agatgatgag tcatattagc attgagtacg
     1921 gtttcatttt cagcatccaa tcaaactgac tgagatcttc aggatttttg agacttttgg
     1981 ctagttcatt agctaaagcc cgcaacttat tagatgtcaa ctgtttttca ttcataaatt
     2041 caccttcatt tgatatcaaa ttaaatagtt caacatcaga gaattataca aacttatgta
     2101 caggctccta tgatcttcga atcctgaaga ttatagtgaa aataagtttt cacgttgaaa
     2161 tcccacatag ttagtatatg ataaataatc atggatgacc tgattaacct cttggggtag
     2221 tgcttttata gaagaataaa tattctacta atttaataca tcacctaaaa tagtattgtt
     2281 ttttatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc
     2341 cagatcttac ttataataaa ggggcttggc agtttggata aactattgtt cttattccac
     2401 taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatcctga aatccccgga
     2461 agtattcaat caaacaatta caccataaaa ggaagagggg gccctaatta agttgtcaag
     2521 catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg
     2581 tctttattga tcctacccac ataatatgga cacagtcgta agtgaggctc tggttttcaa
     2641 attgttccgg actgagacca ccacaccaac ggtgccgtcg ccagcgattg taatcgcact
     2701 cgatataact aaacactgtt gcccgcacta tttcccgatg gataatagtg ttctccgtgg
     2761 agacattcca ttttcagcga atgaattttc atctgttgct gtatccagtc atacagtgac
     2821 tgcatcagtg gcgcggctct ggcttttctt gccgccagac gctgttctgc tgaacatccc
     2881 cggacttctg cctcgatggc atacagttca ccgatacgct gcagggcttc cgtggtgatg
     2941 tcggttggca ctcttgcatg cacatcgtgg atttttctcc ggacatgagc catacatgcg
     3001 gcttccgtta ttctgccgga ttcgtacaac gcccggtaac caccgtaagc atcggcctga
     3061 agcactccgc tgtaaccggc cagatgattc tgtggatgga tacctttccg gtccggtgag
     3121 tacgcgaacc agaccgccgg aggc
//
LOCUS       DADLZM010000245         3135 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLZM010000245 DADLZM010000000
VERSION     DADLZM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.250"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            805..999
                     /locus_tag="JNY73_004050"
     CDS             805..999
                     /locus_tag="JNY73_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY8049842.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESETGT"
     gene            1385..1620
                     /locus_tag="JNY73_004051"
                     /pseudo
     CDS             1385..1620
                     /locus_tag="JNY73_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1645..1779
                     /locus_tag="JNY73_004052"
     CDS             1645..1779
                     /locus_tag="JNY73_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY8049843.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2024..3133)
                     /gene="fepE"
                     /locus_tag="JNY73_004053"
     CDS             complement(2024..3133)
                     /gene="fepE"
                     /locus_tag="JNY73_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY8049844.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          928 a          773 c          653 g          781 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc cccccctaaa aagcggtgtt ggcgcagcta
      421 caacaccgcg ccgacaccgc ttttttaaat atcacaaaga gagcaagaag aactaatttg
      481 tcataactct ctatttataa agaaaaatca gaaaaaactt gtttttgcgt ggggtgtggg
      541 catttcaggc atgagaacag actcgcgttt ttccggagta tcagcggaat tattgattaa
      601 gaaacactcg acgcagagcg aaattttatg caacagcggc tgcactgcgc agggattatt
      661 tatcagttga tatgctgtgc catgatacgt cctaaagaaa caggacagga tgtgcaattg
      721 taataccgtc gcccgcaacg tcttacaatt accacctgct cagggccccc cccgacaccc
      781 ggaactgacg gagaacaacg gaaggtgaac aaacaacaac aaactgcgct gaatatggcg
      841 cgatgtatca aaagccagag cctgacactg cttgaaaaac tggatgctct ggatgccgac
      901 gaacaggcgg ccatgtgcaa acgactgcac gaactcgcag aagaactcca gaacagcatc
      961 cagattcgct ttgaggccga aagtgaaaca ggaacataac gaagctgacg gaggcggtca
     1021 cggctttccc gtgggcgttt tcgtcagcag acaagcccgt cagggcgcgt cagggggttt
     1081 tagcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tatatactgg
     1141 cttaatcatg cggcggcaga gcagatggtg agagaacagc gacatatgag gtgtgaaatg
     1201 ctgcacagat acgtaaggag aaaatacagc ttccggcgct cttccgcttc ctcgctcact
     1261 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtgt ctgctcacgc aaagacggat
     1321 atacggttat ccacagaatc aggggataac accagaaaaa acatgtgagc aaaaaagagg
     1381 ccccgaaaaa aggccgcgcc ggtggcgttt ttccataggc tccgcccccc tgacaagcat
     1441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggacttaa agataccagg
     1501 cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     1561 tcattccgct gttatggccg cgttcgtctc attccacgcc tgacactcag ttccgggtag
     1621 gcagttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1681 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttaac gccattggca
     1741 gcggccactg gtaaacagga tgtgcgagag atgtattgag agttcttgaa gtggcagcct
     1801 tactgaggct acactagaag gacagatttg gtgacatgtc tcgcacaaga ctgttaccac
     1861 ggttcagaag ttccccaact tactgaacct tcgaaaaacc acctccccag gtggtttttt
     1921 cgttttctag agtagaagac tacggctgta agggaatgat ctcaagaaga tcctttgaac
     1981 tttttataaa agttgcttat aaaatgatgc atcagcacaa cttttactca atcacactca
     2041 tctttctggc ctccatggca tgacgtaaaa gtatatagcc acatgcaagc atccctccaa
     2101 tgacagaaga aagaattaca attatggcat tacttggccc atcctttctg accggtaacg
     2161 ttgggctcaa ttgatactga aatggcataa aatcaacatc ccccactttc gccacaacca
     2221 attgattcac aacatattga cgatttcgca actccccact caattccgaa acatccttga
     2281 ttgatttttt gatgttcaat ttagttgcta taccatcaga accgagagcc acaggaaaat
     2341 ctgggtcatc cttcataatc tgaccattgc tgtatacagg tttttttatt ccagccgcat
     2401 tagcaacctc cagtgaataa ttgaggcgtc gaatatctgc attcagctgg tttctaattt
     2461 ttatctcatc cagtgcaaga atttcttttt caacattagt tttaacttct agtttatttc
     2521 ttatatcttc catcaaatcc cttacaacaa gggaagaaac ataattaata tacccagaca
     2581 acacatcgtg agcttcttta cttgttggag cagtaaaact cagagtccat gatacataca
     2641 atgatgactt gtcgtttttc ttactggcat tatcatcaat cgacttcata tttttactca
     2701 actcaacaat tgcacgatgc agttcgagag cgctaatgtt attcttttta ataatctcca
     2761 taacataagg agatgatctc atatactcat tcaacgaatt aacggactga aatttcttaa
     2821 taaacagagc aaatgcgcta tcacgattga tatcaaaatc cactcccaga acatgaagct
     2881 tggaaagctc cttatttaat tcactccatt gaacagactc agcaggagta accacagcag
     2941 aactagtcca tttttgtggc aaaacaaaag aaacaagaaa gccaatacaa gcaaaaaata
     3001 gaatcacccc aacaattttt atttttgctg cccataaaac gctaatcaag ccaaaaaggt
     3061 caatttcttc attaccattc ggcaacatgt tgtaattaga aaaatgttcg tcagagacct
     3121 ttgagatttt cattg
//
LOCUS       DADLZM010000246         3055 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLZM010000246 DADLZM010000000
VERSION     DADLZM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.251"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(105..1784)
                     /gene="bcsG"
                     /locus_tag="JNY73_004054"
     CDS             complement(105..1784)
                     /gene="bcsG"
                     /locus_tag="JNY73_004054"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY8049845.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1781..1972)
                     /gene="bcsF"
                     /locus_tag="JNY73_004055"
     CDS             complement(1781..1972)
                     /gene="bcsF"
                     /locus_tag="JNY73_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY8049846.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1969..>3055)
                     /gene="bcsE"
                     /locus_tag="JNY73_004056"
     CDS             complement(1969..>3055)
                     /gene="bcsE"
                     /locus_tag="JNY73_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY8049847.1"
                     /translation="EEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQN
                     GIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAA
                     TVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVI
                     PWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMM
                     NNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRIND
                     LDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVI
                     NAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT          764 a          787 c          807 g          697 t
ORIGIN      
        1 ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc
       61 ccggatcagg ccgcagaggt taaactctgc ggcctttttc gtgcttactg cgggtaaggc
      121 acccagtcgc cgccgttcag gcgaacgtac ggtttatctt ggtattgaat aactactgca
      181 tttgagttct cggagaccgg tgctgtttgt ggcaacccac tggtgagttt tttccagtca
      241 acattgtctt cggtgaaaat cttgccatca agaacgcgaa ccaccagatc ggagatagcc
      301 aggaagctgc tcggttggtc aatgacaatc ggtgccccct gatgtggtgc cttcatgccg
      361 aagaatttca ccccaacggg gacgtctgtg atagacgggc tagggatatc acgtaggcca
      421 gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac gaccaccatc
      481 actttacgac ccgatttctc cagttcagta aagaaggcgt ccagttcatc aaagaatttc
      541 tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg gataatggtt gccgtcatgc
      601 agtggaagcg tgttgtagaa cgtggcacta cggctgtttt tatctttttc ggtaacgtcc
      661 agccagcggt taagcacggc ggtatcgtca taaaccggcg aaccatcaaa gcccagcaaa
      721 ataaccggca gatttgtttg atccatcaat tcagtctgca tgccgccatt ttcgcgaact
      781 tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg ggtaaagccc
      841 agtttcgaaa ggttatcaaa cagatagcag tcgttatttg ccggttgata cagattagtg
      901 tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc actgtaggag
      961 gtggcggagt taaagttctt gaactcaata tcgaaatgcg accacagtgg atgcgacatc
     1021 aacccggcgg cttctatatc cgaccaggaa agcgaacaga tgttaatcac cagtagttca
     1081 aatggctgag catcagcggg cagcgaagac gggaaggtcg atttacgttt cgcctccgcg
     1141 ttatagaaat tattcagcca ggcgttaagg ttcgccgttg ttggcggtgc agtttgtgcg
     1201 ggcatatcac ccactaccgg tgcgccaccc gtcgccgcaa cggttgccgc tgcgttacca
     1261 cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc acaaggagaa acttggtccc
     1321 gccagggtaa gtacgttcag ccatagcagt atggcaacca caaaaacggt aatgcgaatc
     1381 cattgtgaca ggaataacca ggccactaat aaaacaaaaa tggccccaat catctgccag
     1441 ttaataaagc gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc cacctgcgaa
     1501 ccctggctca ttatgctttc cgggccaagc aaccaggtgt catgccagaa caaagcaaag
     1561 ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc tgtaacgcgg aatgggcatc
     1621 agcagaaacg cggcaaacac caaattgagg agcgggtgga agttaagata tcccgcccac
     1681 aacaggccaa acttaaccag aaaatagaag ttccagccgg aaaggccgcg ccagtattgc
     1741 cagagggaag aaggcatggc ggtattttgc gtaaattgag tcattttttg gttgccctgg
     1801 ctttttccgt gcggcgtaac gtcccggccg gtttaacata acgaggttta gcaaagaaca
     1861 aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc agcgggaaaa
     1921 atatcagtgc gcaaacgata ataatttcaa tgatatcgct gatggtcatc atgatgagcg
     1981 ctccacagca tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt ggcgaccatc
     2041 gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca ttccccattg
     2101 ttctggggca agcaggcgca tctgcaccag ctcggcactg atttgatcat cttcaaacca
     2161 gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat tcaacgcggt
     2221 atccagatcg ttaatccgac agaatgagag aaacagcacc agccgattac cgccaatggt
     2281 catgatatcg ccggtacggt tagggcgaca tagcgtcagg gcttgctcaa cgcggatacc
     2341 cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg gcaggcaaga gagggttatt
     2401 catcatgttg ttgacggcat tacagaacac atcccacttc tggaaaccac gcagtttgag
     2461 cggctgggtc attgacagca aggtagtgat atcttccggt acatagcgac taaacttctg
     2521 cccttgcacg ctttcgatca tcgtcagaca acgggagagt ggcgcattcc acggaataac
     2581 catatttgca ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc tagcggtatt
     2641 ttcccgcacg aggattttca tcgcactacc gcgctggcga cgcagggtat gaatgctgcg
     2701 ggccagtggc tcgatttgcg cattttgctg taaagaaaag accaccgtcg ccgcctgagc
     2761 ggtacgggct tcattgaaca ggacttcgtt attgttgaac agttgccagt gttccgatag
     2821 cggcggcgca ccttccagta cagcaacatt actcagaatg cgtttttcgt cgctgcgtgg
     2881 ttggatctcc gcctcttcgc tttgaactaa tgtccagata ccattttgtt gctgaacgct
     2941 aagctgctga cgggcgctga cccctttttc gttgcaccag aaggcaatat ccagcaaatg
     3001 ttggtcaccc tgaaaacgca aactggcaag accaaaaagt gaacggtatt cctca
//
LOCUS       DADLZM010000247         3038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLZM010000247 DADLZM010000000
VERSION     DADLZM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.252"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            53..1084
                     /gene="ynaI"
                     /locus_tag="JNY73_004057"
     CDS             53..1084
                     /gene="ynaI"
                     /locus_tag="JNY73_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY8049848.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRERKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1135..2748)
                     /locus_tag="JNY73_004058"
     CDS             complement(1135..2748)
                     /locus_tag="JNY73_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8049849.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKGRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          790 a          707 c          672 g          869 t
ORIGIN      
        1 cgccaggggt ttccggcatg acgacaatta tctggaggag cctgataaaa caatgatcgc
       61 tgaactgttt acaaataatg cgcttaatct ggtcattatt ttcggtagct gcgcagcatt
      121 gattctgatg agcttttggt ttcgccgtgg aaatcgtgaa agaaaaggat ttttattcca
      181 tgcggtgcaa tttttaatct acaccataat tatcagtgct gttggtagca tcattaatta
      241 tgttatagaa aactataaac tcaaatttat caccccaggc gttatcgatt ttatctgtac
      301 gtccctgatt gcggttattc tgacgattaa gctatttctg ctgattaatc agtttgaaaa
      361 acagcagatt aaaaaaggtc gcgatatcac cagtgcgcgg attatgtcgc gcattatcaa
      421 aatcaccatt attgtagtgc ttgttctgct ttatggcgaa catttcggca tgagcctttc
      481 tggcttgctg acctttggtg gtattggtgg tctggctgtc ggtatggccg gtaaagatat
      541 tctgagtaat ttcttttccg ggatcatgct ctatttcgac cgtcctttca gtattggcga
      601 ctggatccgt tcaccggaca gaaatatcga aggtacagta gcggaaattg gctggcgaat
      661 taccaaaatt acgacctttg ataatcgtcc attgtacgta ccgaactcgc tgttttcgtc
      721 gatcagcgta gaaaacccag gacgaatgac caaccgccgc attaccacga ccattggttt
      781 acgttatgag gatgcggcaa aagtgggcgt tattgtcgaa gccgtacgtg agatgctgaa
      841 aaatcacccg gccatcgacc agcgacaaac cttactggtt tatttcaacc agtttgctga
      901 ctcgtcattg aatattatgg tttattgctt taccaaaacc acggtatggg ctgagtggct
      961 tgctgcacag caagacgttt atttgaagat tatcgatatt gtacagtcac acggcgcgga
     1021 ctttgccttc cccagccaga cgctgtatat ggataacatt acaccgccgg aacagggtcg
     1081 ctaaatcaga catcactggc gttatccaca agggtaacgc cagttcccat cacatcaatg
     1141 cttcacaata tacatagtcc gactgtacgc tacatcttca gggttattaa tgggataacc
     1201 tttcagccac ggtttgatta atcgtccatt ggtatattga taaattggtg caatcggtgc
     1261 ttgctccatg aggatttttt ctgccgcgtt gtaatcggca ttacgcgctt taacggtatt
     1321 ttccgtcgat gcctgggcca gaactttgtc gtatgccgga ttgttaaagc gtgaaatatt
     1381 tcctgaatgc attgacgtta ataatgtcag gaaagtggag ggttcattat aatcccccac
     1441 ccatgaggcg cggataacat caaaattgcc ggtgttacgg ctatcgatat aggttttcca
     1501 ttcctgattt tgcaatttaa catctacgcc aaggttcttt ttccacatcg atgctacagc
     1561 aatcgcaatt ttttgatggt tttctgaagt gttatacaaa agcgtcagct tcagcggttt
     1621 ttgcggacca taaccagctg cgctcaacaa agtttttgcc tgcgcattca gttcttcctg
     1681 actcatttgt tcaaacggcg aaggttccgg cgtaaatccg gcggtaacat ctggtataaa
     1741 atgccacgct ggcttttcgc ccgtccccaa cactttttcg gtcatcaggc ggcgatctat
     1801 cgtcatactt aatgccaggc gaacgcgctg atctgccgtc ggcccttttt gcgtgttaaa
     1861 ggcataataa taggtcccga gctgcggtgg cgtataaacc tgccccggaa tatccttcaa
     1921 cagcttctga tacatatttt tcgggaagga ttcggtgata tcaatatccc ccgcgaggta
     1981 acgcttagtg gctgcggatt cctgattaat tggcaggaag gtcacttttt gcagcaccgt
     2041 tttggcgtta tcccaataat gggtattcgg taccacgacc agtttttcat tgactacgcg
     2101 ccctttaaga acataagcgc cattgccgat cagatttccg ggtttcgtcc actctttacc
     2161 gctttctacg ttggcttttt gcaccgggaa gaaggcaaag ttagcggtta aattcacaaa
     2221 ccacggcaac ggtttatcaa gctgaatttt caaagtatgg gcatcaactg cggtgacgcc
     2281 aagctgatca ggcgtagctt taccatcaat aatcgcctgt gcgttgttga ttcccgccag
     2341 cgcggcaaac catgcaaatg gcgacaatgt ttttgggtcc accagacgtt gccagctgta
     2401 gacaaaatct tgcgccgtta ccggtgtgcc atccgcccat tttgcgttat cgcgcagggt
     2461 aaaagtccag atacggttgt cattactttt ccattgagtc gcgacgccgg ggacaatctc
     2521 ccctttttcg ttctgattca ccagaccttc aaacagatcg cgaatgacct gaatctctgg
     2581 caggcccacg gctttagcgg gatccagcga cgcaggctca tctttaatgt ggcgcaccag
     2641 ctcctgcttc tctgccagta ctgtgccgct cggaacttct gcagcatacg aaagagaaat
     2701 gctgctgacc aacagcgcac aacacgtaac tgaaacagag tgcttcataa gtttccctct
     2761 aaccgacatt aaaattgatg cgtaattatt tgtttcgcct taaagaaatg caaacaactt
     2821 ccatcaaaaa agtgacaaag gaagcatcaa atgttttaag tatcaatata tcaaatcaga
     2881 tttagtgtaa ataatagacg ccaaaatgcg gcaaagcaca tagaacaccg aacactacgt
     2941 tcgggcttcg cacgttcaaa ccagttttcg gtatcataat cagcatctgt cgccattgta
     3001 cgaagaataa aaaataacca aaacgttgaa aggaactg
//
LOCUS       DADLZM010000248         3002 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.253, whole genome shotgun sequence.
ACCESSION   DADLZM010000248 DADLZM010000000
VERSION     DADLZM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.253"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            182..2050
                     /gene="kup"
                     /locus_tag="JNY73_004059"
     CDS             182..2050
                     /gene="kup"
                     /locus_tag="JNY73_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY8049850.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2217..2636
                     /gene="rbsD"
                     /locus_tag="JNY73_004060"
     CDS             2217..2636
                     /gene="rbsD"
                     /locus_tag="JNY73_004060"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY8049851.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2644..>3002
                     /locus_tag="JNY73_004061"
     CDS             2644..>3002
                     /locus_tag="JNY73_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY8049852.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          675 a          712 c          787 g          828 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattactc aaaggggggg ctttttcttt
       61 gagccaggtt aatgtggccg catttaggag tacgattttg ccgttaatcg tgcatactgt
      121 gcgctttttt gtggaccaag ggactaagca cacatttcat atttcaacga aagactagtc
      181 tatgagcact gataataagc aatcattgcc cgcgattacc ctcgcggcga ttggagttgt
      241 ctacggcgat attggtacca gcccgttata tacacttcgt gaatgtttgt ccggccagtt
      301 tggttttggc gttgaacgcg atgccgtgtt tggcttttta tcgctgatct tctggctgct
      361 aatctttgtg gtttccatta aatatctcac cttcgtgatg cgggcagata acgccggtga
      421 gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc
      481 aatgctggtg attatggggc taatcggcgg cagctttttc tatggtgaag tcgtcataac
      541 acccgctatt tcagtgatgt cagccattga aggtctggaa atcgtcgccc cgcagctgga
      601 tacctggata gttcccctct caattatcgt tctcacttta ttatttatga ttcaaaagca
      661 tggcacggct atggtcggta agctgtttgc gccgatcatg ctgacctggt ttttgattct
      721 ggcaggactg gggttacgta gcattattgc taacccggaa gtgctgcatg cgctgaatcc
      781 gatgtgggcg gtgcatttct tccttgaata caaaacggtt tcttttattg cattaggggc
      841 agtggtgctg tcgattacgg gggtcgaggc gctgtatgct gatatggggc actttggtaa
      901 gttccctatt cgcctggcgt ggttcaccgt cgtattacct tccttgaccc ttaattactt
      961 cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt aagaacccgt tcttcctgtt
     1021 ggcaccggac tgggcgctga tcccactgct gatcatcgcc gcactggcga cggtaattgc
     1081 ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt caggcggtac gtctgggata
     1141 tttgtcgccg atgcgcatta ttcacacctc cgaaatggag tcagggcaaa tctatattcc
     1201 gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg attgtcagct ttgagcactc
     1261 cagcaacctg gcggcggcgt acgggattgc ggtgaccgga accatggtac tgacgtctat
     1321 tctctcgact accgtggcac gtcagaactg gcactggaat aagtattttg ttgcgctgat
     1381 cctgattgct ttcctttgtg tcgatattcc attgttcacc gctaacctcg ataaactgct
     1441 ctccggcggc tggttgccat tgagcctcgg tactgtgatg tttatcgtga tgaccacctg
     1501 gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa catggtaact ctctggaagc
     1561 gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg cggtgtatat
     1621 gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat aaccttaaac ataacaaggt
     1681 attgcatgag cgggtgattt tgttaactct gcgcaccgaa gacgcgccat atgtccataa
     1741 cgtccgtcgg gtacagattg aacaactgtc gcccactttc tggcgcgtgg tggcaagtta
     1801 tggttggcga gaaacgccaa acgtggaaga agttttccac cgctgcggtc tggaaggatt
     1861 aagttgccgg atgatggaaa cctccttctt tatgtcgcat gagtcgttga tcctcggcaa
     1921 acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct
     1981 gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt atcgaactgg gtactcaggt
     2041 cgaaatctaa cgccagacgc ctcctttctt cataaggggg cgtttttgtt ttcatggtta
     2101 atcaccatgt aaaacgtttc gaggttgatc acatttccgt aacgtcacga tggttttccc
     2161 aactcagtca ggattaagct gcggatcagc gaaacgtttc gctgatggag aaaaaaatga
     2221 aaaaaggcac cgttcttaat tctgatattt catcggtgat ctcccgtctg ggacataccg
     2281 atacgctggt ggtgtgtgat gctggtttac ccatccccaa aagtacaacg cgtatcgata
     2341 tggcattaac ccaaggtgta ccttctttta tgcaggtgct gggcgtcgtc acaaatgaaa
     2401 tgcaggtcga ggctgccatt atcgcggaag agatcaaaca acataatccg caactccacg
     2461 aaacgttgct cactcacctt gagcagctgc aaaaacacca gggaaatacc attgaaattc
     2521 gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca
     2581 gcggagaatg ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc
     2641 gtcatggaag cattacttca gcttaaaggc atcgataaag ccttcccggg tgtaaaagcc
     2701 ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga tggcgctggt gggcgaaaac
     2761 ggcgcgggta aatccaccat gatgaaagtg cttactggta tctatactcg cgatgccggc
     2821 acgcttttat ggctggggaa agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc
     2881 gggattggga ttatccatca ggagctgaac ctgatcccgc agttgaccat tgccgaaaac
     2941 attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat
     3001 gt
//
LOCUS       DADLZM010000249         2992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.254, whole genome shotgun sequence.
ACCESSION   DADLZM010000249 DADLZM010000000
VERSION     DADLZM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.254"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            43..357
                     /locus_tag="JNY73_004062"
     CDS             43..357
                     /locus_tag="JNY73_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049853.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            639..1205
                     /locus_tag="JNY73_004063"
     CDS             639..1205
                     /locus_tag="JNY73_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049854.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1410..1526)
                     /locus_tag="JNY73_004064"
                     /pseudo
     CDS             complement(1410..1526)
                     /locus_tag="JNY73_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1732..2286
                     /locus_tag="JNY73_004065"
                     /pseudo
     CDS             1732..2286
                     /locus_tag="JNY73_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2626..2992
                     /locus_tag="JNY73_004066"
                     /pseudo
     CDS             2626..2992
                     /locus_tag="JNY73_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          849 a          648 c          681 g          814 t
ORIGIN      
        1 ttatagaagc tgtcagcggc tacgtcccag ccaaatcact tcatgctcca gcgccagaga
       61 ggcaaggttg ggtttgctca gctgccggtt gattttgttg cgattgaacc ggacagcgtc
      121 cagggcgtcg gcaaaagagc gaatctcacc aatcgttgtt tcattatccg cattaacgat
      181 agcttcaaga aacgccaagg ttttatcgaa ttcatcagca acagtggctc cgggcatacc
      241 gtgaccgttt atacaaaacg gcgttttcag cgtggagttt tcatgaatag cctcaacacg
      301 aatgtcgtga agccagtgat gtatcggcta cgatcctcag ctgatgccag accataggac
      361 tgacactacg aacagcaatt ttaatgacgt aaatcttcat aactacaact aacagggcat
      421 cattatgaat gatgccggtt attttaccgc tgttggtccc ttcatttagg gactgcccca
      481 agttttttca tactctcttt ctcagagagt ttagtctctg ggattccaga cggtccatca
      541 ttgagccaag ggagtaaaga actcaaagca tttacagaat tgtttgaatc agctaaaata
      601 atgacaatgt tactatttta catgacaaag tgattataat gcttggaaca tcttttaata
      661 attttggaat cagtttatct cacaaaagat atttttcagg aaaggtggat gagattatcc
      721 gctgtacaat ggggaaaagg atagtaaaaa tatcatcaac caaaataaat actagtattt
      781 tgagctctgt ttcagaacaa atcggtgaaa atattacaga ttggaaaaat gatgaaaaaa
      841 aagtctacgt atcccgggtg gtgaaccaat gtattgataa gttttgtgca gaacacagcc
      901 gaaaaatcgg cgataatctc cgtaaacaga tatttaaaca ggttgagaaa gattatcgca
      961 tttctctaga catcaatgct gcacaaagtt ccataaatca tcttgtatca ggaagcagtt
     1021 atttcaaaaa gaaaatggac gaactttgtg aagggatgaa tagaagcgtt aaaaatgata
     1081 caacaagcaa tgttgcaaat cttatttccg atcagttttt tgagaaaaat gtgcaatata
     1141 ttgatctaaa gaaattacgt gggaatatga gtgactatat tacaaattta gaatcgcctt
     1201 tctgatgaaa gaacatcccg agtaaggaaa ctaagaaaag taaataccca ggaattgcct
     1261 gagcggtgct atatttctcc tcgcaagtaa gtcctaatat attgaattgt tgtatagcca
     1321 gtttttgtat tttcctagct catggatgtg ggatccctct cccctttctc tgctcaaagg
     1381 taagcggctc gtcagaatca tattgatatt tactgagaag tcagatcaac tttccaaggc
     1441 aacagatcgc gtacccggtt tgccggtcag tcctggatat gttcaataac gtaacgcagc
     1501 catttttctg gctccacatt gttcagacgg catgtgccga tcagcgagaa caacaccgct
     1561 gcatgttcgt caccgctgtc ggaacctgcg agtcgagtca gccggagaga gtttcactct
     1621 ccggctgctt acggaaccgt acgtgacact ctcgcgtcat acggctcttg atcactcatt
     1681 cgatgcacgt agtaaatacc agtgagcaaa ttgatcttac ccagcaatac ggtcagttac
     1741 cgcgcacatg gcctgcctgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
     1801 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagg ctggctgtat
     1861 ctggctgtgg tcattgacct gtggtcacgt gccgttattg gctggtccat gtcaccacgc
     1921 ctgacggcgc aactggcctg cgatgccctg cagatggcgc tgtggcggcg taagagaccc
     1981 cggaacgtta tcgttcactc ggaccgagga agtcagtact gttcagcaga ttatcaggcg
     2041 ctgctgaagt ggcataatct gcgtggcagt atgagcgcaa agggctgctg ctacgataat
     2101 gcctgcgtgg aaagcttctt tcattcgctg aaagtggaat gtctccacgg agaacacttt
     2161 atcagccggg aaataatgcg ggcaacagtg tttaattata tcgaatgtga ttacaatcgc
     2221 tggcgacggc acagttggtg tggcggcctc agtccggaac aatttgaaaa ccagaacctc
     2281 gcttaggcct gtgtccatat tacgtgggta ggatcactta cgccctgtgg ttgaaacgtg
     2341 agcatattgt tttcagatgc tgttgtcttg cttcagagtg atctgtcctc cggatttacg
     2401 caaagttatc cacaggcact gtgcatttaa aaaacttatc ttttcttctt gattttaaaa
     2461 actcagatcg tcatatcatt atgataaaca taataaaaaa ctctaaacat gagaaggttt
     2521 ttaacgtaag gtgagaggat ttttaagtgt atccatagat taaaaatgac aatgcctcca
     2581 attagttgaa ttaggggtta taatacccgc ttttaaggtt ctttcatggt cacagttaac
     2641 tttcattgcc cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaagga
     2701 catgatcgat tccgttgccg tgactgtcac cgggtgtttc agctcactta ctgttacgag
     2761 gcccgtaaac cgggagttaa aaaccaaatc actgaaatgg ctttcaacgg tgccggagtt
     2821 cgcgatactg cccgtacgct gaaaatcggc atcaataccg tcatacggac attaaaaagc
     2881 tcccgccccg gcggataaca tctgcgccag tcgctcacgc tgatgttgcc cttatctgtg
     2941 aacttgatgg gcagtggagc ttcgttggca gcaaggcgca acaacactgt ca
//
LOCUS       DADLZM010000250         2943 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLZM010000250 DADLZM010000000
VERSION     DADLZM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.255"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            316..576
                     /locus_tag="JNY73_004067"
     CDS             316..576
                     /locus_tag="JNY73_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY8049855.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            800..874
                     /locus_tag="JNY73_004068"
     CDS             800..874
                     /locus_tag="JNY73_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY8049856.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            867..1724
                     /gene="repA"
                     /locus_tag="JNY73_004069"
     CDS             867..1724
                     /gene="repA"
                     /locus_tag="JNY73_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY8049857.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2424..2615
                     /locus_tag="JNY73_004070"
     CDS             2424..2615
                     /locus_tag="JNY73_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049858.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          775 a          784 c          720 g          664 t
ORIGIN      
        1 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc agcagtcacc
       61 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
      121 gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
      181 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgccacaa
      241 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttgag
      301 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt gagtcaaaag
      361 cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc cgctgttgca
      421 agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct gaaagatctc
      481 atggttgagt actgcgagag agaggggata acacaggctc agttcgttga gaaaatcatc
      541 aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt ggcgtagcat
      601 gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg tggcagggaa
      661 ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa tcttctttaa
      721 ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa caatcaagct
      781 atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt gctcgctcct
      841 tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat taccgccagg
      901 taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt ccgttctgcg
      961 ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc attcatgtgg
     1021 cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt cgccgggcca
     1081 ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac cgcgtccagt
     1141 gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct gccggaaaac
     1201 tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga ctgattacct
     1261 accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc acgttcacat
     1321 ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg cgccgcagtc
     1381 gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg ggtatggatg
     1441 aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac cagacagagc
     1501 ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg cagcgtcagg
     1561 atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg cgcttcactg
     1621 ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc atgattctgt
     1681 cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga cctcctcaga
     1741 ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc acaaaaaatc
     1801 agcgctacat acaaaaaaca acctcatcat ccaccttctg gtgcatccgg tcccccctgt
     1861 ttttgataca aaacacgccc cacagacggg gaattttgct tatccacatt aaactgcaag
     1921 ggacttcatc gtaaggttac aaccgttcac gtcataaagc gccagccgtc aaccctacag
     1981 ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta attacattca
     2041 tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acagatatgc acctcccacc
     2101 gcaagcggca ggccccaacc gaagccggtt tagttacaac atgcaaacat aaacccctaa
     2161 aaaacccaag tccaacgcag aactgaaacc acaacgcccc tcctcataac tgaaaagcgc
     2221 cgccgccccg cccgaagggc gggaacagag tcgcttttaa ttatgaatgt tgtaactaca
     2281 tcatcatcgc tgtcagtctt ctgcctggac gtatcgagta cacgctcgta agcggccctc
     2341 acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc gggaccactc
     2401 cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttagca gggctgggga
     2461 tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc ggttttactc
     2521 cggtatcaca tgtaaccaca gcgtgtcgcc ttccatgccg ctggcgcggc atcagaataa
     2581 tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt cgtttcaggg
     2641 atatcgttaa gacttgagcg ataggaatta aaaccccaaa aagattaaaa aaacaccaca
     2701 aaacggatgt ttcttcaaca ccacttttgc tacatatgaa cggaaccgac gattaaactg
     2761 gatggctctg attgattcag ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg
     2821 gacgcaactt cccctctgcg gctctcagcc gcaccaccgc atccgggcca gcggctcatg
     2881 catcaggacc tgctctgcca gacggtagcc ccgcttcagc cccgtaaaac gcatctgact
     2941 ccc
//
LOCUS       DADLZM010000251         2936 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLZM010000251 DADLZM010000000
VERSION     DADLZM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.256"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..215
                     /locus_tag="JNY73_004071"
     CDS             <1..215
                     /locus_tag="JNY73_004071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049859.1"
                     /translation="CADLNIPFFVDARNKKLVFWFVRVDDEGYPEIARCMEREFATIP
                     AGINADGMYCPECGTVHWPDGVIPPF"
     gene            263..692
                     /locus_tag="JNY73_004072"
                     /pseudo
     CDS             263..692
                     /locus_tag="JNY73_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            879..1043
                     /locus_tag="JNY73_004073"
                     /pseudo
     CDS             879..1043
                     /locus_tag="JNY73_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1537..2115
                     /locus_tag="JNY73_004074"
     CDS             1537..2115
                     /locus_tag="JNY73_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049860.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2224..2482)
                     /locus_tag="JNY73_004075"
                     /pseudo
     CDS             complement(2224..2482)
                     /locus_tag="JNY73_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2466..2936
                     /locus_tag="JNY73_004076"
                     /pseudo
     CDS             2466..2936
                     /locus_tag="JNY73_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          884 a          548 c          775 g          729 t
ORIGIN      
        1 gttgcgcaga cctgaatatt ccgttctttg tggatgccag aaacaaaaaa ctggtcttct
       61 ggtttgtacg tgttgatgac gaagggtatc cggaaattgc ccgctgcatg gagcgggaat
      121 ttgcgacaat tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg
      181 ttcactggcc ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt
      241 tcagcaggga aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct
      301 ggaacattat gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg
      361 agaagaaaac cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc
      421 ggataagggg ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg
      481 aagatgtggt gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga
      541 gagggatgta tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc
      601 agatgcggtt tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg
      661 agccgctggc tcgaatacgg tggtgcggct gagagtcgca taggggaagt tgcgtccatg
      721 ttgaggggaa cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata
      781 ttaagtgtct ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg
      841 gtgctttttt aatcttttgg ggatttaaat tcctatcgat gaactgtcga tatgaaaaag
      901 cgagcatcat actgacgtca aacaaagggg tcgcagactg gggagaaatg ttcggagatc
      961 acgtgctggc aacagcgatc ctagatcggc tgctacacgc tgaatatcaa aggagagagt
     1021 taccggttaa aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt
     1081 gatgatgaaa tggtggaaaa cggacagcat cagtaacgaa agtatcttag caggcatgaa
     1141 aatcgtccgc atcccctaaa cggtcaaaat gcttctgccg tgaaggaggg agaatgccat
     1201 ctcatcactt atatctggcg aatgaaacac agatggtcgg ccttgccccc aaacacacgg
     1261 cggtgcaaaa tggaatggca gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg
     1321 ttatgttcga acctgacgtg ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat
     1381 tataatgaat ggcatcctca tagtgcgcag ggttatcgct tgctacggaa atatctgaag
     1441 cgacgtacca gtaatgggga aaatgatagc tggtttctga aaatatagag gaaaatgtga
     1501 tatatatatc ctgttaatat ttaaggagaa tataaaatga aagtctcgtt taagtcttta
     1561 ggctatattt ttcatgatat atataataaa aaacacacta tcgatgagtt caacgatgtg
     1621 gtaagaaaag cagttttatc aggtaaaatt aacgagctga atgcatgtca taaagtggca
     1681 atttttcttg ccgaaaaaga caatgagata acaaagaaag acaaagccaa gataatagat
     1741 acattaactg aaaactattc cattgaattt caacaattaa tgaatattag tgagcgcacg
     1801 ttaaattcat ctttatatat tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt
     1861 gaaggaaaaa tatgtcatac tgcatatgta aaatcatcag ataatagcat ggcatattac
     1921 catgcaaatg gtagcagcat tgacaaatat attacggata tgtgcggcct tatttgtatg
     1981 aggcatattg agtctactgg cattattttt tatatgcttg atgaaaaagt tttatctgcc
     2041 attgctgaat ttatgaatga aaaaggatgg cgggctgcgt tttgttcagc aaagaactta
     2101 tataaatgtg tttaatttta gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt
     2161 cgcgttgctt cagcagggcc tcagcctgtt caaccagact cagttgcagg tcgtgacgca
     2221 gatttagtgg tggtggttga ttgtcagata ccagccgatg atcttgtagt gcatatcatc
     2281 agactttgag aagcatatgg ttttgcgggt aagccgctta agatgtgtcc gcaggttcag
     2341 attatgtcgc tctatccgct gagtgcattt cttgcttata acatgactgg ttgaggacaa
     2401 taaagttcgg tataccggct caggcatcgg tcatgtagaa ggcaatgcta aactggctca
     2461 gcaaactaag agcagcgcgc atccacttac ccaatgcggt tgagaaaata accttcgatc
     2521 gctttcatgt ggcgaaacaa ccgggtgagg tggtggataa aacccgccag aatgaacatc
     2581 cgcatcttcc tgttgaaagt cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca
     2641 gcgataaatg gatgaccgaa tccaggcagg aaaagctgat atggctgcgt gcgcagatga
     2701 agctgacgag cctgtgctgg gcgctgaaag aactggcaaa ggatatctgg agcagaccat
     2761 ggagcgagga aagacgcaac gactggcaga gatggctgcg gccaacagtg acgtccccat
     2821 gatgaagaat gtggctaaaa cgatagggaa aaggttgtac ggtatcctga acgccatgcg
     2881 ccatggcgta tcaaacggaa atgcggaagc actgaacagc aagatcaggc tgctga
//
LOCUS       DADLZM010000252         2789 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLZM010000252 DADLZM010000000
VERSION     DADLZM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.257"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            23..985
                     /gene="yeeE"
                     /locus_tag="JNY73_004077"
     CDS             23..985
                     /gene="yeeE"
                     /locus_tag="JNY73_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY8049861.1"
                     /translation="MYIAKNNRMFYALLIAISAQSVGVFALIQAGLLTYEAGAFPWLG
                     TVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSAVMRSPHASGLNQT
                     LQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMKELKKPKLKVATLPPRRTGIAHI
                     LFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGVTSPTANILQFLVAGDVKYINW
                     GVFLVLGIFVGAFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGNGL
                     VMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            999..1226
                     /locus_tag="JNY73_004078"
     CDS             999..1226
                     /locus_tag="JNY73_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY8049862.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNY73_004079"
     CDS             complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNY73_004079"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY8049863.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          643 a          660 c          742 g          744 t
ORIGIN      
        1 cctgactggt ggttttcgcg atatgtatat cgcgaaaaat aatcgcatgt tttacgcctt
       61 gctgattgca atttcggcac aaagcgtggg ggtttttgcg ttaattcagg cgggcctact
      121 gacttacgaa gccggggcgt tcccgtggct tggtactgtt ataggtgggt atcttttcgg
      181 gctgggaatt gttctggcgg gtggatgtgc caccgggacc tggtatcgcg cgggtgaggg
      241 attgatcggc agttggatcg cgcttttcac ttatatggtg atgagtgcgg tgatgcgttc
      301 tccacacgcc agtggtttaa atcaaacctt gcagcactac actactgaac ataactctat
      361 tgctgagact tttaatttgt ctgtgtggcc gttggttacc gttttgctgg tgataacgct
      421 ctgggtggtg atgaaagagt tgaagaagcc aaaactcaaa gtcgcgacct taccgccgcg
      481 ccgaaccggg atcgctcata ttctgtttga aaaacgctgg catccctttg tcactgctgt
      541 actcatcggt ttgattgcgc ttttagcctg gcccctgagt gaagcaaccg gacgcatgtt
      601 tgggttagga gtcacttccc caacggccaa tattctgcaa tttctggtcg cgggtgacgt
      661 gaaatacatt aactggggcg ttttcctggt gttagggatc ttcgtggggg catttattgc
      721 agccaaagcg agccgtgagt tccgcgttcg cgcagctgat gcacaaacaa cattacgtag
      781 cgggttaggt ggtgtactga tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg
      841 taatggactg gttatgactg caatgatgac ctggcagggc tggattggcc ttgtatttat
      901 gattctcgga gtctggactg cgtcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg
      961 actggcaact gctgcggcaa attaagtaag gtgtaaagat ggcgattaaa aagctggatg
     1021 tggtgacgca ggtctgtccg tttccactca tagaagcaaa agcagcactg gcagaaatgg
     1081 ccagcggcga tgagttggta attgagtttg attgtaccca ggcaactgaa gcaatcccac
     1141 agtgggcggc agaagaagga catgccatca ccgattatca gcagattggc gatgcagcgt
     1201 ggagcatcac cgttcaaaaa gcctgactca acattgtcct ccgccgtacc agcggcggcg
     1261 gcttcaaatt agacgatctc ttccgcgtac tgccaaagtg cttttaacag cgccactttc
     1321 tctttgtcat cggcatattg ttgtaccagc atctgcaatt catcagcata accctgcaaa
     1381 aactctggcg tgaagacctg gtggcggtgc tcctgccagc gttgctgctc ggcataatcc
     1441 agcgtccccg ggaagttgcg tgcccgataa ttgaacaaca acttttcaat tcgtttatca
     1501 acaaaagtga tatccagtgc cggtaaatta cgcggctcgg tttccagcac aattttcatt
     1561 gctgcacgat cggcgtcact gaaaaagcca ttataaagct gtgcatccac attatctgaa
     1621 ggtgtaaacg gttcggcttc cgcgaatatc gctaccactt tttcacgcac ttgcggattt
     1681 tcacgcagaa tttttaggtt atcgaggcaa tgctgacgat taattcccag tcggtcggca
     1741 tcttccgggc gtagcgtatt cgcctgggcc agtaccggac atttattgat atgcaccagc
     1801 ttaaccggaa cggcggcgtt atcgccaaga tcggcttttg cggtatataa acgctcgcgc
     1861 aatgtgtcga tatccagctc cagtaatggc gaaatgtctc ctgccaaatc cgccataatt
     1921 acggcattgc gattttcagg atgccacgcc agcggtgcca cccagctggt attgccacgc
     1981 catgccccaa acattccgga aacgtgagcc aggggtttca tctgcggaac atcaatcaac
     2041 gccatcagtt tgtgtttatt acgatgggta aagagataat caaacaggcg tggctgacgc
     2101 gtttttacca gtttcgccat cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg
     2161 ttgctatgtt caataccatt cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg
     2221 tcatcatttt caggccagtt tattccttcc gggcgcaggg cataacaggc acgcataaca
     2281 tccagtaaat cccagcgcga gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa
     2341 ttacgataaa aaacgttgcg tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga
     2401 atacaggtct tcggtacggt aaaaagcgag tgaatacggg cggcaaacgc ggcttcgttt
     2461 tctcctttcg cccgtgcctc ctgcggggta ataccggtaa ttaatactgc tccaggccgg
     2521 ggtaaatagt catccgcggg cttgcagtaa aagacttcgg gttcgccgat gacattgaat
     2581 tcgtcatcgg tgcgaatggc tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca
     2641 aaggtttcgt aatcgtgaaa gaggaaggta ggttgtttat ctgtgtctgt cattgtctag
     2701 cattgtccac tgtagttaat taactgtctg ttataactcg aaaagcaacc cttttttcac
     2761 ctgagtatgt ctacgaacgt caaatatag
//
LOCUS       DADLZM010000253         2772 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLZM010000253 DADLZM010000000
VERSION     DADLZM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.258"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..992
                     /locus_tag="JNY73_004080"
     CDS             <1..992
                     /locus_tag="JNY73_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8049864.1"
                     /translation="QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEY
                     GNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKT
                     VEQYGNEVVYIQYSSGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAM
                     PYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDP
                     RYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
                     APKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYIC"
     gene            1026..1349
                     /locus_tag="JNY73_004081"
     CDS             1026..1349
                     /locus_tag="JNY73_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8049865.1"
                     /translation="MTARAIAMLPILTGNVGISGGNSGARESTYTITIERLPVLDNPV
                     KTSISCFSWTDAIDHGPQMTALRDGVRGKDKLDVPIKFIWNYKDLKRLLVRVTRDLSR
                     PEVLH"
     gene            complement(1942..>2772)
                     /locus_tag="JNY73_004082"
     CDS             complement(1942..>2772)
                     /locus_tag="JNY73_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049866.1"
                     /translation="LKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGER
                     PEVVTVPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTI
                     NARMVDVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHE
                     SQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDG
                     ENITVVLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA
                     "
BASE COUNT          696 a          720 c          736 g          620 t
ORIGIN      
        1 tacaacaggc cagcgaaaaa gtgatctggg gttcctgttc cgtcaactgt ggtagccgct
       61 gtgcacttcg tctacatgtt aaagataatg aagtgacctg ggtggaaact gacaataccg
      121 gcagcgatga gtacggcaac catcaggtac gcgcctgttt gcgcggtcgc tccatccgcc
      181 ggcgtattaa tcatcccgat cgcttgaatt acccaatgaa acgcgtgggt aaacgcggtg
      241 aaggcaaatt cgaacggatt agctgggatg aagccctgga tactatcgcc agtagcctga
      301 aaaaaaccgt cgaacagtat ggcaatgaag ttgtatatat tcagtactct tcggggattg
      361 ttggcggcaa tatgacccgc tcttcgccat cagcctcggc ggtcaaacgc ctgatgaact
      421 gctacggcgg ttcactcaat cagtatggct cctacagcac cgcgcaaatt tcctgtgcca
      481 tgccctacac ctacggcagt aatgatggca acagcaccac ggatattgaa aacagcaagc
      541 tggtcgtgat gtttggtaac aacccggcag aaacccgcat gagcggtggt ggcatcactt
      601 atcttcttga aaaagcgcgc gagaaatcga acgccaaaat gattgttatc gatccgcgat
      661 ataccgatac ggctgccggt cgtgaagacg aatggctccc tattcgcccg ggcaccgatg
      721 ccgcgctggt tgcgggtatt gcctgggtat tgattaacga aaatctcgtt gatcaacctt
      781 ttctcgataa atactgcgtc ggctatgacg aaaaaacctt acccgcagat gcacccaaaa
      841 atggtcacta taaagcctat attcttggtg aaggtgacga taaaacagca aaaacgccgc
      901 agtgggcttc gcaaattacc ggtatcccgg tagaccgtat catcaaactg gcgcgtgaaa
      961 ttggcacaac aaaacccgct tatatctgct agggctgggg gccgcaacgc caggcaaacg
     1021 gcgagctgac tgcacgcgct attgctatgt tacctatttt gacgggcaat gtcggcatca
     1081 gcggcggaaa tagtggcgcg cgtgaatcga cctataccat tacgatagaa cgcctaccgg
     1141 tgctggataa tccggtcaaa acgtcaatct cctgcttcag ctggacggat gctatcgatc
     1201 atggcccgca aatgacggca ctccgcgacg gtgtccgggg caaagataaa ctggatgtgc
     1261 ccattaagtt catctggaac tacaaggact tgaagcggtt actcgttaga gtcacgagag
     1321 accttagtag gcctgaggtc ctgcattgag tcaagacctt gtgtcattct ttgtgccaga
     1381 cttgtgtcat tagacgggat gtctagacgg ctggtcagga cccccggtgg cctctttgtc
     1441 aggactcagg gtggcaccgg ggaacattgg tgtcttaatt gtattgaatg aggtctctaa
     1501 cttttggtcc aaaactcatc gttagctgct cgccagctca cagccgaacc cgaactgtaa
     1561 gcacgaccac cagcaccagc ttcagcagga agacgacaag atagacttta tgcaggtcca
     1621 tgcgccctcc tgtgtctatt gtaccactgc actagataac tagtacaaaa taaagtccag
     1681 accgaaggcc cagaccatag ttaattaatc acattgataa tccaccacct gaagtcctgc
     1741 caacattcag tccagcctta cccatgctag gagtcttaac cggggtgtcg ttctgtgtct
     1801 ccaccactgg tgccgcatca ggctctacag gaagctccac agcaggcgca gggaccggag
     1861 gtgatacgga tgtaccacca ccagcggaga agcccttagc gatggaccgg aggcctttac
     1921 taatgctctt acccattgct gttacgcttc ggtcttggtg aacttcaggc gtttgactac
     1981 aggcaggact gcggttgcag cgccagtgtc cagcttaact gtcgtagcag cttcagcgac
     2041 aacgaagacc ttaccgagga tttccaagtc gtgggactga gagtcgccat cacgtgaaag
     2101 gacgaccgta atattctcac cgtcgtgacg gaagcagccg aagctcaggg ccgtgaagcg
     2161 tttagccagc atgaacacac ctttgtcctc accgttgtaa tacgcgatgt cggtacccag
     2221 catatccacg atagcgtctt caccagagaa gccctgttta gcagccagac gggacagcgc
     2281 tttcagttgg gactcatggg cgatgatgcc gtaatcagac acttcagagc cgaaagagga
     2341 ctcggtgttc tcaaacagag cgtccatgat agcgtctgcc ttagtggtca ggcttccagt
     2401 caggactggc agctctacgg tcttcagcag cttggaggac tccagaacgt caaccatgcg
     2461 agcgttgatg gtacggacca cattctcaca gatgttcata gcgtcctgct ggagtgtgcc
     2521 atgagtgaaa ccagtggagg acttggcgtc ccacagttta gtttgctccg agatgaggat
     2581 tgcctgttcg ataggttcga cgacaatctc ctgaccagac ggaacggtca cgacttcagg
     2641 tcgctcacca ccgcgtacag ggacgaccac agagcggtca gacagggtgt gggcgatgat
     2701 tggggtaggc aggctattgc gggtaggcag gacttctaca gggaacggaa tgttatcctg
     2761 agcgtcttta ag
//
LOCUS       DADLZM010000254         2732 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLZM010000254 DADLZM010000000
VERSION     DADLZM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.259"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..2623
                     /locus_tag="JNY73_004083"
                     /pseudo
     CDS             1..2623
                     /locus_tag="JNY73_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT          582 a          749 c          797 g          604 t
ORIGIN      
        1 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
       61 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
      121 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
      181 ggcgctgacg cgtctgtggt cgccagtgat gacggcattg ttgcgcgcat tcctgacact
      241 gatggtaaat tgcccgacgc cgcgattttt ttgtttgaac cagaaaagtt gctgcaaatt
      301 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
      361 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
      421 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
      481 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
      541 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
      601 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
      661 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
      721 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
      781 ttgcaacgac tggctcctgg cagaagagcg aaaggtgaag aaggattgtt cgacctgctg
      841 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
      901 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc tgcgatgatt
      961 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
     1021 gtacgactac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
     1081 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
     1141 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
     1201 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
     1261 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
     1321 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
     1381 gcctcaacat cgccaggcgt ttatgagggc gtagatggcg tgatgcgggt gatcgaacag
     1441 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
     1501 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
     1561 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
     1621 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
     1681 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
     1741 acgcgataaa atcggcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
     1801 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
     1861 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
     1921 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
     1981 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
     2041 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
     2101 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
     2161 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
     2221 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
     2281 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tggcctgcac atccggcagc
     2341 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
     2401 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
     2461 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
     2521 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
     2581 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
     2641 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
     2701 tgcttcaacg agcggcctga ctgcaccatc tc
//
LOCUS       DADLZM010000255         2713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLZM010000255 DADLZM010000000
VERSION     DADLZM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.260"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..283)
                     /locus_tag="JNY73_004084"
     CDS             complement(<1..283)
                     /locus_tag="JNY73_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HAY8049867.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLI"
     gene            complement(270..902)
                     /gene="mxiE"
                     /locus_tag="JNY73_004085"
     CDS             complement(270..902)
                     /gene="mxiE"
                     /locus_tag="JNY73_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY8049868.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(1032..1460)
                     /gene="mxiM"
                     /locus_tag="JNY73_004086"
     CDS             complement(1032..1460)
                     /gene="mxiM"
                     /locus_tag="JNY73_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY8049869.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(1444..1851)
                     /locus_tag="JNY73_004087"
     CDS             complement(1444..1851)
                     /locus_tag="JNY73_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY8049870.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(1844..2539)
                     /locus_tag="JNY73_004088"
     CDS             complement(1844..2539)
                     /locus_tag="JNY73_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY8049871.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(2481..>2713)
                     /locus_tag="JNY73_004089"
     CDS             complement(2481..>2713)
                     /locus_tag="JNY73_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein mxiK"
                     /protein_id="HAY8049872.1"
                     /translation="GGKISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALE
                     GNYTPVERNLSRLNEGMQYAKRHFTGIQTSCL"
BASE COUNT          879 a          525 c          335 g          974 t
ORIGIN      
        1 atatgagtcc cacaagtaat gtcagttttt caagcatttc ttctggatta gataaatcaa
       61 actccccaga tattctcttt tttgcagctt gtttgctaac aacaatagga taatttaata
      121 acgcagaaaa acgttcaaaa aaagatccta ccgtatcact ttgagcaaca tatttcgcta
      181 tgtcattttg ttctaataaa tgagagtcaa tattattagc attaactatc aatggtaaca
      241 atacaatcaa gagagtcaaa gatttaatat taaatttttt catttatttt tttcactaaa
      301 aaagtaatat tcgataattc tcttgcagaa aagcctagtc gtgtttttat ctcatttgaa
      361 aaatgggatg ttgatgcata cccattattc atggcagcag aagtgattgt ttgattgtgc
      421 aaaaatacat ctaataaacc atttactaac cgccaagtgt tcagttgttc tttgactttt
      481 gcccctaaag cttttctaca taaagatcta aaataagcct ccgacacacc ataatgctct
      541 gtcagactct ttattcttac ctccttttct ttctcaatag ttctcagaag ataaagaacc
      601 aaatgatatg actcagtcat tcgtatcata gccagtgcat tacttacatt ctggtcagat
      661 gaaaagatga atcttaacat taacttatca agcatcgatt cggtattaca actatcccga
      721 agatcattct catgatagaa atgaatacat ttttcagcat ggctatgctc tactagagca
      781 ccactgaaat agtcccaaaa agcaagaaat tttgcacaac tattaatgtt aaagcgataa
      841 tgatagttca cattatcact gaattttata ttttgatttc ggacaaaaaa aaacccttcc
      901 atgctggagc cagctcaatt atgttacgtt ctttatcttt tacaagctca acgttaactg
      961 gttcatgtcc actagagtaa atgtaaaaca tattacttgt atttagacct ttatatttac
     1021 tcacaataca attatatccc tcgaattaat tttaatttaa cttccccatc cttctctata
     1081 aggatggtat catttatctt attacctttt aaaacaaccc cgtctttcag catagaaaat
     1141 agtttatcta tagctatctt ttgtgcatcc atgaagcacc catctacttc cttaacatta
     1201 aagttgccca tgaatttacc tgcattaaaa ctataaaccg ccttaccact aatacattta
     1261 gagtaaacat ttatactttt attggttgta ttaaacgacc ataataaatc atttggaata
     1321 gaaaaataat ccttacttac aggaacaata tgccattctt tttcagaatt agatgatgac
     1381 tttaaagcac acccactcag tgttaatagc aatatactta aaataaatat tttcaactta
     1441 ttactaccat gtcgaatcat ctgcctctct ccctgcttca aagacactaa ttaatgaatc
     1501 gaaggttgta gtcttaatat agaaatcaat attgttatat atggacaatg atgcataaaa
     1561 tccctcatgc aaatcgacat attgatttgc atctatgagt ttattaagaa gtgaactttt
     1621 ttgtgcagct gtaagataag gaaaacgtga attgaataat tgttcaatct tattgtgcag
     1681 ttcatcatta tctatggaat ttaattcact ctgtgagaaa tattgcatta tttcatagat
     1741 tatatcctca tcaaatgatt gcgaagatga taatctctca tttaaattaa caaattcttc
     1801 actatttaat ctttgctgca gagcattgct tgcatttatt tgattaatca ttaacaggat
     1861 tctcttttaa caagtcttct gcaatttgtc tcagtttctt atgtgctaat tctttgaaat
     1921 attttttatc attttttgtt aatttttccg caagttctat tttaacacct gatattacat
     1981 cctgaggact aaactccact acttgattac cactacagaa tatataatta ttcccttcag
     2041 aatattttaa aataatcttg acatctgagc gatatttatg gagatcaagt tctaactttt
     2101 tactcagtgc taaattacac ttgggcaaaa ccacttttaa ttctgttact gtattacgca
     2161 acgaactaag ccactgctcc aataaagtgc taatgatcct ctcatcatgt aaattttctg
     2221 aaagcaaact agttatatag tcctcaatat ttcttctgtt ttcattttgc tttaacttcc
     2281 attcacaaat atagtcaata atatgctcaa aagcagtctg tataccaatt tgataaccat
     2341 cacatacagc atctatacgc agtgattcag cttttttcgt agcctccttt ataacacaaa
     2401 ttgctttctt cttactctca gtgtctctat cttttatggt tttacataat tccttttcaa
     2461 ttctctttat aacaacacca tcataggcat gatgtctgga taccggtaaa gtgccttttt
     2521 gcatattgca taccttcatt taatcgggac aaatttcttt ctacaggcgt ataatttccc
     2581 tccaaagcaa ttggattaaa taataagttt attctttcat atataccaac agatatattt
     2641 tccttccacg caataagatg ccaaaaacca cttcgaatag cggcctcccc ttgcttactg
     2701 attttgcccc cat
//
LOCUS       DADLZM010000256         2697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLZM010000256 DADLZM010000000
VERSION     DADLZM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.261"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="JNY73_004090"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="JNY73_004090"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY8049873.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JNY73_004091"
     CDS             complement(1725..2096)
                     /locus_tag="JNY73_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY8049874.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JNY73_004092"
     CDS             complement(2119..2463)
                     /locus_tag="JNY73_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY8049875.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          667 a          649 c          673 g          708 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactactg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttggacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLZM010000257         2678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLZM010000257 DADLZM010000000
VERSION     DADLZM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.262"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..710
                     /gene="zupT"
                     /locus_tag="JNY73_004093"
     CDS             <1..710
                     /gene="zupT"
                     /locus_tag="JNY73_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY8049876.1"
                     /translation="VLGQKPSNRLLAFSLGFAAGIMLLISLMEMLPAALAAEGMSPVL
                     GYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLPKSIKRTAILLTLGISLHNFPEG
                     IATFVTASSNLELGFGIALAVALHNIPEGLAVAGPVYAATGSKRTAILWAGISGLAEI
                     LGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVDELMPLAKEIDPNNNPSYGVLCG
                     MSVMGFSLVLLQTAGIG"
     gene            complement(768..938)
                     /locus_tag="JNY73_004094"
     CDS             complement(768..938)
                     /locus_tag="JNY73_004094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049877.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1199..1852)
                     /gene="ribB"
                     /locus_tag="JNY73_004095"
     CDS             complement(1199..1852)
                     /gene="ribB"
                     /locus_tag="JNY73_004095"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY8049878.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(1957..2105)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2167..2517
                     /gene="ubiK"
                     /locus_tag="JNY73_004096"
     CDS             2167..2517
                     /gene="ubiK"
                     /locus_tag="JNY73_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY8049879.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT          720 a          663 c          613 g          682 t
ORIGIN      
        1 gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt ttcgctaggt tttgcggcgg
       61 ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa
      121 tgtcgccggt gttgggttat ggaatgttta tcttcggtct gcttggctac tttggcctgg
      181 accgcatgtt gccgcatgcg catccgcagg atttaatgca aaaatcggtg cagctgttgc
      241 caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg cataacttcc
      301 cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga tttggcatcg
      361 cactggccgt cgcgttgcac aatatccctg aaggtctggc agtggcaggc ccggtttatg
      421 cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga ctagcagaaa
      481 ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg gtggtcatgg
      541 cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc
      601 cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt
      661 cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa aaacaacatc
      721 agaagttgtc cagcacatgg acaacttctg ccagcttcac gaaagcctta ttctaccttt
      781 tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct
      841 ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaacc
      901 agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt aatatttaac
      961 aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat tcatatggtc
     1021 gcataataac ggaacaatta tatgattttg atcataatga aaaagaatgc ctgatattaa
     1081 tgaaattttc tcatagaaag agaagcggaa ttaacatctt gcatagcagg gcaaaataaa
     1141 aaccgaagcc agctacaact tcggtttctt gattaaggca gtaattaagc agcggttttc
     1201 agctggcttt acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg
     1261 ccatattgtg tttattggca aactcaatgc actctggtgc acgcgccatc gtgccatcgt
     1321 cattagtcag ctcacacagc acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa
     1381 tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga
     1441 aaacgtggcc cgggcgattc agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa
     1501 cggtcgtaat acgatcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg
     1561 tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcaa
     1621 gttgtttacg gcgatcttca gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg
     1681 tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt
     1741 cacggtcttc atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat
     1801 tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa
     1861 aaacctcact aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag
     1921 ccaaaataac gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc
     1981 taaccgtcgg ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc
     2041 tcgcgggctt tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa
     2101 taagcggatt cactataact ctaatgatta gcggcagcaa cgcatagctt cacataattc
     2161 tggtttatga cttaccctta tcgcactaca atggtactca acacctatca gtacagggaa
     2221 actacaatga ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg
     2281 cctaaaggaa tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa
     2341 gctcagctga cgcgcctcga ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc
     2401 ctgttacgga ctcgtgagaa actggcgctg cttgaacaaa gaatcagcga actggaaaat
     2461 cgcagtactg agatcaaaaa gcagccagat ccagagactc tccccccaac gctgtaaaca
     2521 tttgtcttta atgcactgta aattatgaat taatcctcta atttacagtg ttttctatta
     2581 cattatcgct taatcccatt cagagtatca tcacaaaaat gaatgattgc aattgaaaat
     2641 ataattgtta tgtattcttt aagttttact taatcgat
//
LOCUS       DADLZM010000258         2668 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLZM010000258 DADLZM010000000
VERSION     DADLZM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.263"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(43..990)
                     /gene="icsP"
                     /locus_tag="JNY73_004097"
     CDS             complement(43..990)
                     /gene="icsP"
                     /locus_tag="JNY73_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY8049880.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2371..2523)
                     /locus_tag="JNY73_004098"
                     /pseudo
     CDS             complement(2371..2523)
                     /locus_tag="JNY73_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2579..>2668
                     /locus_tag="JNY73_004099"
     CDS             2579..>2668
                     /locus_tag="JNY73_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HAY8049881.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
BASE COUNT          819 a          515 c          455 g          879 t
ORIGIN      
        1 caatttttag ccatgaaaac gtaatcaact cggtgaacca attcaaaaaa tatactttat
       61 acctgcggaa gtgagaaaac caatatattc aatacttgca ctatttttaa tggtgccaga
      121 tatattggta ctttgctcat aaagagatgt atcacctttt ttattagaaa tgtaatttct
      181 ggagccttct atataaaatt ttgcactggg ggtgacatag tatccaatat tcgcagcaac
      241 agagcagtaa ttttggtttt ttatttcatc tttaaaaatt gtctcagtct gatagtgttt
      301 atctgtatca gatgaaagaa cccaaccact atatttcagt tctgctccaa actcaaaatt
      361 ctcatggcgg taattagcag ttaatccaat ataaggtatt ttgaagagct gtttgtaacc
      421 tattgttctt tcaccactag gatgtgcccc ttttttattc ctgttccccc cgttttcact
      481 ataaatataa ctccctccca ttgcattaaa actgtaacga ctctcttggt aacctgctat
      541 tagtccaagt cgataatcca aattatttaa taaccaacct ttaagattca gctcgaattc
      601 gttagcatca cggagatgcg tattgggatg ccagctctgg tcggtccata cctgaggggt
      661 attggaattg ttccagtctt tatcaaccat gcttgctttc tggttaccta aagtagtcca
      721 accggaaact ccgaaagaca cttttggaat tagcttccaa tcgatgccac ctctaacaat
      781 agtcgcatta ctgtatttcc agtccagttg actaattttt ctcccccctt ccttgggatg
      841 ataaacgcgt tcttttgttt tgccactcag agatcctaga ctaatatccg tactgatgtt
      901 gtccggtatg aaaagtggat agttagtggt agcatgtgta gtaaagatcg caggtacaca
      961 aagtgcaagt acaaggaatt ttgatttcat cgagaactct acttttttgg ttgaaatgtc
     1021 catgatcttc ttactttata agataaacgt caatgccaag cattatttta attaaatatt
     1081 tgtttatgtt accaagaaca tatcttttat tataatattt attgaacttt tgtaaaaata
     1141 aaaaaatacc tcattgtgcg aataaagtaa cgggggccat agatttctct acctaccttt
     1201 tggtgttcat acctgaggct gatagtttca ggattccgcg acaattctac tcaccttctt
     1261 gttctctcgc caatatgaaa tcaatcagaa aagcgttcca taagaacaac gataagacaa
     1321 taaattgtca tcacggctgc aatagccaac gtgcctttca gtaccaacat gacaacctcc
     1381 tgtatttgac gtaacgtgtg ctgataaata tgtatttgta tctcaactga ctcaatacaa
     1441 cacttaagta tagtttttca gattttgttt ttcccgtgaa atattatgga aatggcagaa
     1501 gcaatacatt gaattccgat tcgagtgaat acggctagcg ggcacctcac tttagcactg
     1561 aagccatcaa cttttatttg acttatttca atggaaagct ttctcactat cagtacaggt
     1621 aattcccatc cttttaaagt cgagagtcag actgcgacta atacagttaa agtgaccaaa
     1681 ctcagttatc ctctacacat tttacctcat gtttacttgg acaattcatt ggtgctttta
     1741 taaaagaata aatattctac taatttaatg catcacctaa aatagtattt tttataatag
     1801 aactgtatct atagtatatt attaaagctt aattatttat tatgctttaa tgtggttgtc
     1861 cgattaagga ctaatccttg aaaacgccgt atagggcggg agttcactct attgcgataa
     1921 cgtaaccgta caatattgtg cgtttcccaa attgaccact actgaactat tctgggattt
     1981 aaatgcaata aaatggttgg ttgaaggtcg tggtgatgat gttagtcctt ttgagaatat
     2041 tccagtgcca gatcttactt ataataaaga ggcttggcag tttggataag ctattgtcct
     2101 tattccacta atacaaactt tttgtaaagt atattaaata tacttcattt catactgaaa
     2161 tccccggaag tattcaatca aacaattaca ccataaaagg aagagggagc cccaattaag
     2221 ttatcaagca tgtttatgat acctgcaggg tataaatttt ctggctgcaa gcagacacat
     2281 ataaaacgtc tttattgatc ctacccacat aatatggacg cagtcgtaag tgaggctctg
     2341 tgagcctacc cacgtaatat ggacacaggc ctaagcgagg ttctggtttt caaattgttc
     2401 cggactgagg ccgccacacc aacggtgccg tcgccagcga ttgtaatcgc actcgatata
     2461 attaaacact gttgcccgca ctatttcccg atagataaag tgttctccgt ggagacattc
     2521 cacttaggaa caattatatt gttcatggta atgtttatat ttgagtatag agattaaaat
     2581 gattagtccc atcaagaata ttaagaacgt atttccaata aatactgcaa atactgaata
     2641 tatagtgaga aatatttatc ctagagtt
//
LOCUS       DADLZM010000259         2648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLZM010000259 DADLZM010000000
VERSION     DADLZM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.264"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1475
                     /locus_tag="JNY73_004100"
     CDS             <1..1475
                     /locus_tag="JNY73_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY8049882.1"
                     /translation="AIGLSIAAGGAALTWYQVAETDLSRTAAAAHEAVSVTERLLDEA
                     QGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLSCSSWEGADPVREF
                     ISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVADNMTLLNLRRPLS
                     LRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPLTLYFHDGALSLLL
                     SVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDASTGEIAGVEVLAR
                     WVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLPPLTGDGKRWHISFNITQS
                     HIMETGFIAECLTFIDAFTPGSITLTIEPTEREPFDNSDEMRCQLKRIHDAGIALALD
                     DFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIEMANTLKKEIIAEG
                     VETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT          682 a          558 c          618 g          790 t
ORIGIN      
        1 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
       61 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
      121 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
      181 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
      241 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
      301 gggagttcat ctccgctggc gacaaccaga cgctgattct ggcgacagac gattatatat
      361 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
      421 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
      481 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
      541 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
      601 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
      661 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
      721 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
      781 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
      841 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
      901 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttaccg cctctgacag
      961 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
     1021 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
     1081 ctatagagcc cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
     1141 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
     1201 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
     1261 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
     1321 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
     1381 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
     1441 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
     1501 cattgaacct gtgagggccg ccccctttat tactcagggg ggctttcaac gttctgaatt
     1561 aagatgtttt atgactcact gagcaggatg tgagttccaa cgtaatctcc gctattttta
     1621 ctgtttttca tttgctgcct catctatccc ttcgccttgc ctctgagttg cgttaaaggc
     1681 tgatgtactg cctttaaccc gttcaataca tagccttttt gtcaatcatc atcccgaaga
     1741 gcgttgcact gtgccttcca tagctgtggt tgtcctctca atctgtcagc ccatttatgg
     1801 aaaatcttaa cgaagcaaca atgagatatg taaaaaaagg gccgatttaa tcattcatta
     1861 agacaggtca ttttttctta ctgtgagggc ggtaagcttt tctgtcggta acaggttggg
     1921 agatgctggt attatgagat ttttctaccg tcctgcggcg gaatatgtga aaaatagaaa
     1981 tgattattat tattgcatgg ttatttttag tttgtgatgg tggagaataa tcctattccc
     2041 gtgaccttgt tttttacatt ttatttaact gcatggtata tgcattttgg acggtgaaag
     2101 caagaagcta ttaggcgcta taggtctttt gacggttttt gaatttcccc tcctgatatc
     2161 tctgttatat ttttgtgaat ttctccaatc caaatcaaag tcccaataag tcatggatgc
     2221 atggttaaaa taactatcat ttgtgtaacc tttggtgctt ataacttttc tttattcttt
     2281 tttcaggatt acctggaggg attgaccaaa aaaacattca ttgaatgata tttttctaat
     2341 taatgaggtt ttctaagctc gttgaaataa aaaatatatt ttaatcatgg atttgcatgc
     2401 gtttctttcg ctttttttac acaagttgac attgaactca gcaactataa gaatagccac
     2461 atcccagacg gtgaccgata aaggaaatac cgttaccata tttaaatgaa ttggaaaata
     2521 tcaccgattt gaagtcgact cgttcgggta attattaata ctgaatcttt aatgcttatt
     2581 tcttaagctt gttaacaacg ggaatgaata atgaaagtgt ccaaaattgc actggcaatg
     2641 ggtcttag
//
LOCUS       DADLZM010000260         2622 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLZM010000260 DADLZM010000000
VERSION     DADLZM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.265"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..104)
                     /locus_tag="JNY73_004101"
     CDS             complement(<1..104)
                     /locus_tag="JNY73_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY8049883.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="JNY73_004102"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="JNY73_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY8049884.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="JNY73_004103"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="JNY73_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY8049885.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="JNY73_004104"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="JNY73_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY8049886.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2423..>2622
                     /locus_tag="JNY73_004105"
     CDS             2423..>2622
                     /locus_tag="JNY73_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY8049887.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSA"
BASE COUNT          768 a          552 c          497 g          805 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcatc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atgcctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gcggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagacttc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac gggcgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gc
//
LOCUS       DADLZM010000261         2622 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLZM010000261 DADLZM010000000
VERSION     DADLZM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.266"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(61..600)
                     /locus_tag="JNY73_004106"
     CDS             complement(61..600)
                     /locus_tag="JNY73_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8049888.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /locus_tag="JNY73_004107"
     CDS             complement(665..1213)
                     /locus_tag="JNY73_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8049889.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNY73_004108"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNY73_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY8049890.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          744 a          598 c          536 g          744 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata agtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga tt
//
LOCUS       DADLZM010000262         2536 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLZM010000262 DADLZM010000000
VERSION     DADLZM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.267"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..350)
                     /locus_tag="JNY73_004109"
     CDS             complement(<1..350)
                     /locus_tag="JNY73_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8049891.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKG"
     gene            complement(415..1365)
                     /gene="virK"
                     /locus_tag="JNY73_004110"
     CDS             complement(415..1365)
                     /gene="virK"
                     /locus_tag="JNY73_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY8049892.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            complement(1370..2458)
                     /gene="capU"
                     /locus_tag="JNY73_004111"
     CDS             complement(1370..2458)
                     /gene="capU"
                     /locus_tag="JNY73_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY8049893.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPTNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            complement(2461..>2536)
                     /locus_tag="JNY73_004112"
     CDS             complement(2461..>2536)
                     /locus_tag="JNY73_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAY8049894.1"
                     /translation="LFYYTTPFFSSLLAFHKGPRLPDD"
BASE COUNT          769 a          588 c          476 g          703 t
ORIGIN      
        1 cctttccaga acactcgttt ctggaacttt tccggaccac gaattgccag ttctgccatc
       61 atcactatgg attgcaatgc tgtggcaaac atattgtcga caattatttc ccgttctgta
      121 tcactctttt ccgggaaaca aagcgataaa ttaatcgtcg ccctgcgacg agcttttctg
      181 ctcaatctgc ccacccaacg ccctattttg gccaagacag gatctctgaa tgaaggggga
      241 aacattgaaa tacctgcaat cattcccaaa acgaaccatg taaaccagta aacaggggaa
      301 agataatttt tcttaaattc aggaataaac tcggatttgt attttttcat tatttagtgc
      361 gaatgcccct accaaaatca tcattaatat tttattcaga gaatacatca aaccttaatt
      421 taagtcctga tgttctgagt ttacaggata tgcatccaaa gagcggatag cagtttcatg
      481 gatataatcc agcaaagcgt atcgttttcg atactcagag cgttttttac tagctatatt
      541 gcttagcggc tttcgaacca cctgtgttgg taatttgtac cagtcaccac aggttttccc
      601 tgctacagac tcccagaaat cactgtagac agcaacaaag gttttgcgtt tctgatacca
      661 gtatctcaat tgccggaaaa catgactgtg ctcactgact gccagacact cagagacatt
      721 acaagctttc atcaacgcac aaaatgcttc aaaaataatc cgttttggga aaagcccata
      781 acaggcccgg gttgcgcacc gaataacatc aggtccggta tttttggggg ctccctgtaa
      841 tccgccaata aaagcggtgt gtccattttg agtcagaatg actgaaaatg ttaaccgggc
      901 aatcactatt tgattgaaaa acaatgacaa catcaactct ccttcccggt caaaaccact
      961 gggagaacac tggatatcaa aatcctctcc atctttcccg atgaattgga ctaatgatac
     1021 cgatttttgt ggaaacaaaa attcagaata tttttctggc aaattttgca caaagcgata
     1081 atgctcaaga atatattgcc cccgggacca ggctcgcccc cctttatgca aataaggtcg
     1141 atgaattctt gctggcaacg tgggttgtat tgccagaata tcctcaatgc actgcaagga
     1201 acgcagttcg taataatatc gaatactggc aaccggattt atagcacatc gcaaaatgta
     1261 catgaaccgg aattttctgt gttcccactt ccccgggagt gaatctgagg aaaaaacaat
     1321 ccttttaagg aaaccgataa atgataagtt acttacagaa aacatattgt tacttgtgct
     1381 tcgctaatgt gagtattttt aacgcagttt tattaatatc aaaccgctct tctatatcct
     1441 gttttgcctg gcgagccatc tgataaaaac gcccagggtc attaaaaaaa tcattcaggg
     1501 cgcacatcca tgcgtgctta ttacctgctg gtaacaatgt cccggtctgg ttgttctgaa
     1561 taacatcagg gattccacca atctgactgg ccagtacagg cacagaaaat gccgatgctt
     1621 ctgccagtac cataccaaac gattcgtttt ctgaaggcag aaccaccaga ctggcaaccc
     1681 gatatacggg ggcggcagga aaaacattgt cagcaataaa aacatcatca tgcatgccca
     1741 tactgtcaat ctgatactgt aaatgctccc gcagttcagg cgaaccagac cccacaatca
     1801 accaacagaa ctgtcgtcca ttcattttta aatggaacag taaattcaac ataaattcgt
     1861 gccctttttc cgggcgcaac attcctacct gagcaataac ggggcatccc cttcgggacg
     1921 ccagccaaga aagaacattt gtaggcaaac tgtttcggga atcaacgtat aatttctgga
     1981 aatcaaagcc tggaggcaca acagtaaccc gggttcgaca tccttcctgc tccagatgtg
     2041 tcttcatact tgttccggga acaatcacct catcgcaaaa atgatttatt gaaaaaactt
     2101 ttgtttttcg tgtcaaatat gtcttttgcc tgataattct gaatggatgt ttcctagtga
     2161 ataaccgtac taaaccaaca atattgctat catgcccact gtgacaaacg attgcattgg
     2221 gctgaaaacc atgaactatt ccgagtaatc tccatgcagt agggatgtga agactgtttc
     2281 gaaataacgc gaatgtgata tcaattccca atttactggc ttcaaaagca attttgctgt
     2341 tttccctgca gacaagcaga actgaatgcc ccattttctt cagggccttc atttgagcaa
     2401 cagcctgtaa ttcctgacca cctatattcg gtgatgattc cgtaaatagt atattcatta
     2461 ttaatcatcc ggtaatcttg gccccttatg aaaggccagc aacgatgaaa aaaacggtgt
     2521 tgtataataa aaaagt
//
LOCUS       DADLZM010000263         2503 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLZM010000263 DADLZM010000000
VERSION     DADLZM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.268"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            155..1108
                     /locus_tag="JNY73_004113"
                     /pseudo
     CDS             155..1108
                     /locus_tag="JNY73_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNY73_004114"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNY73_004114"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY8049895.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYSVGQGPMWVVVNAH"
BASE COUNT          692 a          613 c          561 g          637 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgaatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gactggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aataagcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttat tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgccg cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DADLZM010000264         2491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLZM010000264 DADLZM010000000
VERSION     DADLZM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.269"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNY73_004115"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNY73_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY8049896.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JNY73_004116"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JNY73_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JNY73_004117"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JNY73_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..2156
                     /locus_tag="JNY73_004118"
     CDS             1905..2156
                     /locus_tag="JNY73_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049897.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(2176..>2491)
                     /locus_tag="JNY73_004119"
     CDS             complement(2176..>2491)
                     /locus_tag="JNY73_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY8049898.1"
                     /translation="ISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRG
                     IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLA
                     KV"
BASE COUNT          702 a          481 c          575 g          733 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata atctaaatag
     2161 ctgcgcttaa taccgctaca cttttgccag cccatgtttg cctccgggga atgggctgac
     2221 ggtttccata aaatggcgaa cttttttcaa cagttgccac attgagcggc actgatgatt
     2281 acgcgttatt gtgtcgtgaa gtgcctgcca tagccgttca acatgattca cccatggcga
     2341 gtaaaccggc tgataaatgc ccctgaactt cgggttctcc ttcagccagc tctgtgtttc
     2401 ccggcttttg tggataatgt agttgtccac gatcagcgtg atggttttcg cccgacggta
     2461 tgtcgcttta agccgcttca gcaggctgat g
//
LOCUS       DADLZM010000265         2486 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLZM010000265 DADLZM010000000
VERSION     DADLZM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.270"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            33..1085
                     /locus_tag="JNY73_004120"
     CDS             33..1085
                     /locus_tag="JNY73_004120"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY8049899.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMCLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1161..2486
                     /locus_tag="JNY73_004121"
                     /pseudo
     CDS             1161..2486
                     /locus_tag="JNY73_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          590 a          558 c          642 g          696 t
ORIGIN      
        1 ataagttatg tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc
       61 gaggtggaac ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc
      121 agcgcgataa aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta
      181 ttggcggcgg taatccactg accagtaaag tcgccattat tagccgttcc agcgatccgc
      241 gtgctgatgt cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca
      301 cgcctaactg cggcaatatg ctgtctggcg taggggcatt cgccattgaa aatggtttga
      361 ttgcagcgac ttcgccagtt acccgtgtac gtatccgcaa cgtcaatacg ggtacgttca
      421 tcgaagctga tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg
      481 acggcgtacc gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gctggaacga
      541 aaaccggaaa agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga
      601 cctgtatcga tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag
      661 gttatgaatt accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta
      721 tccggctaca agcgggtaaa gcaatgtgct taggtgatgt cagtaatatg gttatcccta
      781 aacctgtgct tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc
      841 cgcattcttg ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg
      901 cattggaagg caccgtcacc cgacaaatcg tcccttctgt aggatatggc aatatcaata
      961 ttgaacaccc cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga
     1021 cgttacgcgc atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttatcttc
     1081 cctgaaaaaa ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata
     1141 cagaaagaac aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag
     1201 cgatcccatg tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg
     1261 tgctttttgg tatttacctg gcggccattg tgggtctggt tatcaagcct ttcccggaac
     1321 ctgtcgtact gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg
     1381 gtgcgtttaa gaccaccgcc gtattaagcg gttactcttc aggtactacc tggctggtgt
     1441 tctcggcgtt taccttaagc accgcatttg tgaccaccgg tttaggtaaa cgtattgcct
     1501 atctgctgat tggtaaaatc ggtaacacca tgctgggtct gggttacgtt acggtattcc
     1561 tcgatctggt actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt
     1621 taccgatcat caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc
     1681 gtgtcggaca ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt
     1741 tctttaccgc aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc
     1801 tgcaaattag ctggggtgga tgggcgctgg ccgccggatt gccggattgc cggattgccg
     1861 gattgccgga ttgccggatt gccggattgc cggattgccg ggcatcatta tgctgctggt
     1921 caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg ataacaaaac
     1981 catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa aaatgctgct
     2041 cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg gggttgatga
     2101 atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg ttacctggga
     2161 agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg gtattatcgg
     2221 cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag tctttaaaaa
     2281 taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt tcctcagcat
     2341 catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt tacctgtatt
     2401 tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg cactgttgtt
     2461 ctccaactcc tatggcggca tggtta
//
LOCUS       DADLZM010000266         2467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLZM010000266 DADLZM010000000
VERSION     DADLZM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.271"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(458..830)
                     /locus_tag="JNY73_004122"
                     /pseudo
     CDS             complement(458..830)
                     /locus_tag="JNY73_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            833..1030
                     /locus_tag="JNY73_004123"
                     /pseudo
     CDS             833..1030
                     /locus_tag="JNY73_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1074..1193
                     /locus_tag="JNY73_004124"
                     /pseudo
     CDS             1074..1193
                     /locus_tag="JNY73_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1443..2309
                     /gene="ospB"
                     /locus_tag="JNY73_004125"
     CDS             1443..2309
                     /gene="ospB"
                     /locus_tag="JNY73_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY8049900.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
BASE COUNT          763 a          470 c          498 g          736 t
ORIGIN      
        1 acttcggggg aaaaacgtgt atttttagtc attctgttta cctctttctc agggcgttta
       61 gtctccagga tttccggggc ggttcaaaaa actcttcgtt gattactgtg gacttactgt
      121 tggcattacc gaccctgaga ccggagaaat aagaaccgct tagatcatcg tggctgttct
      181 cggggtatca agttacacat gggcagaggc tacccaaggt ctactattat cgcctgaaag
      241 cgctgatgga acagtgctat cagggacatg ctgatgaaaa tacctaaata atcagccagc
      301 actctatctt tccaaatcca cagcatagca aagagagcaa aagagcctgt aaattcagaa
      361 attaaggaaa cgcctcttgg tgcacataat ctatcttatg ttaaatgaaa tctaatttct
      421 tgattatatg ctggatcgtt cgtgggtggc tttgccccta tatttccaga cacctgttat
      481 cattggcccg ctgccgcaga tattcccgtg gcgagcgata atccagcgca ctatgcggat
      541 gccattcgtt ataatgctcg aatgcctctg caaggttctt tgctgccgtt aacccgtcag
      601 gtttgggcat gatactgatg tagtcacact ttattgtttt cacgaagctc tttgctattc
      661 tgttactctc cggactccgc accgccgtgt tcttcggttc aacaaagtcc caacatccgg
      721 gtgaactggc gtgtttcatt agcccggtag catgaaccat tatccgtcag tcactccact
      781 ggagacgacg gaagatcgtt gccgaagcgg tgttccaccg ctcccagcat gtcgccaatg
      841 ggagagcatc tacggtctga ttgaactgtt tattcaaatt gtccaccgtc tgtcagtacg
      901 ctctgaacgt cggctggtta aaacactgct cgctgatttt cagagggttc acggtaaaac
      961 agcactttta ttccggattg cggaagcggc tctgaataat ccggatgggc tggttaaaga
     1021 ggttgtgtac catctgcata ttgttgaacc agatcgcagc ggtaagagaa gttcatcata
     1081 cttggcgcag ttacgcgatg tttctgctag aggtgatgcc gtaaagaacg gcagaacatt
     1141 accggaacaa gatagcggtc tacctgcact ggtatcagac ccggggcttc cccggatgat
     1201 ctccacggta ctttaggtac tgtgtctata ttatgtaggt aggatcaatt aactttgtag
     1261 ttgaaattct ttcattgaaa taatgaataa aaaagacaaa ccgcattaaa caatgccaga
     1321 atataattgc atttttgttc cgttttttta aatcatagta tacaaagaag cttgcatact
     1381 atatgaaaat ataacaccct ttactatgat gtttataaaa caatatatgg agtcatgtag
     1441 gtatgaattt agatggtgtt agaccatact gtagaatagt caataaaaag aatgaaagca
     1501 tatcagatat tgcatttgca catataataa aaagggtaaa aaattcatca tgtactcacc
     1561 caaaagcagc attggttttt ttaggagaga aaggtttttg tgatagcaat gatgttctat
     1621 ctattatggg acaacaaata ccaagagtat ttaagaacaa gatgttatat gattatgttt
     1681 ttaaaaatga aaaaagtaaa aatgattttc taaaaatggc tgaatcatgg ctaccacaga
     1741 gtgaaccaat agtaataaat aatgatgatg acgcattgaa tgctgctgct tatttttctg
     1801 taaaaaaagc gaaaataaaa acagtaaacg atactgattt taaagagtat aataaggttt
     1861 atattcttgg gcacggtagt cctggttctc atcaattagg ccttggttcg gaacttattg
     1921 atgtacaaac aatcatttca agaatgaaag actgtggtat tctaaatgtg aaagatatcc
     1981 gttttacttc atgcggctcc gctgataaag tggctcctaa aaattttaac aatgcccctg
     2041 ctgaaagtct ttcttgtatc cttaactctc tgcctttttt taaggaaaaa gaatctttgc
     2101 tagagcagat aaaaaaacac cttgaaaacg atgagtcatt gagtgatggt ctaaaaatat
     2161 ccggctatca tggatatgga gttcactatg gtcaagagct ttttccctac tcacattatc
     2221 gttcaacttc aattcctgct gatccggagc atacagtaaa aagaagctct cagaaaaaga
     2281 cttttattat taataaagaa ctggattagt ataaaatttt taacctatag ttaattgttt
     2341 gcatttaaga tgaagttatt ttatctttag tagaaaagtg cttccctatg agggaaaatc
     2401 acaaatcatc ataatacaga gacaaaacga tacgaacaaa tagataaaaa acatcattct
     2461 tttacca
//
LOCUS       DADLZM010000267         2456 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLZM010000267 DADLZM010000000
VERSION     DADLZM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.272"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            9..2243
                     /gene="xdhA"
                     /locus_tag="JNY73_004126"
                     /pseudo
     CDS             9..2243
                     /gene="xdhA"
                     /locus_tag="JNY73_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            2254..>2456
                     /locus_tag="JNY73_004127"
     CDS             2254..>2456
                     /locus_tag="JNY73_004127"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="FAD-binding subunit; with XdhA and XdhC
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY8049901.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
BASE COUNT          593 a          649 c          647 g          567 t
ORIGIN      
        1 aatcatgcat gcgcgtcgat tccattgcta aggtcaccgg gcgggcacga tatactgacg
       61 attatgttat ggcgggcatg tgttacgcga aatatgtacg tagccctatc gcacatggtt
      121 atgccgtaag tattaatgat gaacaagcca gaagtttacc gggcgtactg gcgattttta
      181 cttgggaaga tgtgcctgat attccattcg ctacagctgg gcatgcctgg acacttgacg
      241 aaaacaagcg cgataccgcc gatcgcgcac tgctaactcg ccatgttcgt catcatggtg
      301 acgccgttgc catcgtcgtg gcccgcgatg aactcacggc agaaaaagcg gcgcaattgg
      361 tcagcattga gtggcaagaa ttacccgtta tcaccacgcc agaagcggcg ctgacagaag
      421 acgctgcacc aatccataac ggcggcaatt tactgaaaca aagcacgatg tcgacgggca
      481 atgtccaaca aacaatcgat gccgccgact accaggtaca ggggcactat cagactcccg
      541 ttattcaaca ttgtcacatg gaaagcgtaa catcgctggc gtggatggag gatgactcgc
      601 gaattaccat cgtttccagc acccagatcc cgcacattgt tcgccgcgtg gttggtcagg
      661 cgctggatat tccctggtca tgcgtacgag tcatcaaacc gtttgtcggt ggcggttttg
      721 gtaataaaca ggatgtactg gaagagccaa tggcggcatt ccggtgaaag tttcccttag
      781 ccgtgaagag tgtttcctcg caacccgtac ccgccacgct tttactattg acgggcaaat
      841 gggcgtgaac cgcgacggaa ccttgaaagg ttatagtctg gatgttctgt ctaacaccgg
      901 cgcttatgca tctcacgggc actccatcgc ttctacgggg ggggaataaa gtcgcttacc
      961 tttatcctcg ttgtgcctac gcttacagtt caaagacctg ctataccaac ctcccctcgg
     1021 ctggtgcgat gcgtggttat ggcgcgccac aagtcgtatt tgcctttgag tctatgcttg
     1081 atgacgccgc gacagcgtta ggtattgatc ctgttgaaat tcgtttacgc aacgccgccc
     1141 gcgaaggaga tgctaatccg ctcacgggca aacgtatttg cagcgcaggg ttgccggagt
     1201 gtcttgaaaa aggccggaaa atctttgaat gggaaaaacg ccgtgcagaa tgccagaacc
     1261 agcaaggcaa tttgcgccgc ggcgttggcg tcgcctgttt tagctacacc tctaacacct
     1321 ggcctgtcgg cgtagaaata gcaggcgcgc gccttctgat gaatcaggat ggaaccatca
     1381 acgtgcaaag cggcgcgacg gaaatcggtc agggtgccga caccgtcttc tcgcaaatgg
     1441 tggcagaaac cgtgggggtt ccggtcagcg acgttcgcgt tatttcaaca caagataccg
     1501 acgttacgcc gttcgatccc ggcgcatttg cctcacgcca gagctatgtt gccgcgcctg
     1561 cgctgcgcag tgcggcacta ttattaaaag agaaaatcat cgctcacgcc gcagtcatgc
     1621 tacatcagtc agcgatgaat ttgaccctga taaaaggcca tatcgtgctg gttgaacgac
     1681 cggaagagcc gttaatgtcg ttaaaagatt tggcgatgga cgctttctac caccctgaac
     1741 gcggcgggca gctctctgct gaaagctcca tcaaaaccac cactaaccca ccggcgtttg
     1801 gctgtacctt tgttgatctg acggtcgata ttgcgctgtg caaagtcacc atcaaccgca
     1861 tcctcaacgt tcatgattca gggcatattc ttaatccact gctggcagaa ggtcaggtac
     1921 acggcggaat gggaatgggc attggctggg cgctatttga agagatgatc atcgatgcta
     1981 aaagcggcgt ggtccgtaac cccaatctgc tggattacaa aatgccgacc atgccggatc
     2041 tgccacaact ggaaagcgcg ttcgtcgaaa tcaatgagcc gcaatccgca tacggacata
     2101 agtcactggg tgagccaccc ataattcctg ttgccgctgc tattcgtaac gcggtgaaga
     2161 tggctaccgg tgttgcaatc aatacactgc cgctgacgcc aaaacggtta tatgaagagt
     2221 tccatctggc aggattgatt tgaggataac atcatgtttg attttgcttc ttaccatcgc
     2281 gcagcaaccc ttgccgatgc catcaacctg ctggctgaca acccgcaggc caaactgctc
     2341 gccggtggca ctgacgtact gattcagctc caccatcaca atgaccgtta tcgccatatt
     2401 gttgatattc ataatctggc ggagctgcgg ggaattacgc tggcggaaga tggctc
//
LOCUS       DADLZM010000268         2454 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLZM010000268 DADLZM010000000
VERSION     DADLZM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.273"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(404..1183)
                     /gene="ipaJ"
                     /locus_tag="JNY73_004128"
     CDS             complement(404..1183)
                     /gene="ipaJ"
                     /locus_tag="JNY73_004128"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY8049902.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
BASE COUNT          742 a          610 c          373 g          729 t
ORIGIN      
        1 agatttattt ttctaacccc aaaagttatg cttagtcaaa gagtaactta taatatccat
       61 acccctcata gggcttacag tttaaattgc gggtataaaa tacatctatt ttcaaataaa
      121 ataaagcata ctccaattac aacgagtgac taaaataaaa atatcatcca agtcaatcac
      181 tgtttgctct gcaaaatatg tcagccttct caaacttacc agcacttaaa tatttaagca
      241 cacaatcatt ccttgaggga gttcacttat ttcaagagaa acatttcgtc ttcccacaca
      301 actaatccgc ccaatatagc taaatttgta ctcagtaata tcatttgtta tgcccccccc
      361 gccccaaaat attcaagctc cttcacagat aaaaaacgac aatttacaaa gcctcattag
      421 ttataactat ggaaatacct attttagtat atcctataaa gttagaatta cttcttcttg
      481 cacctaattc catagttgaa aaacaagaat aattttctcc tacagctgga tccatatagc
      541 ttccatcagg tctacacaac acccagtgga ggccgacagg aactccaata aaggatacag
      601 caacagcttc caaaactctt tggttagaag ataacacatc tcgttgatgt actatattac
      661 atcccatgcc aattaataag tcccttgcat cgggataaat aaaactaatt acttgcgaaa
      721 aaatgttact ttcttcaacg acataagcat tcaatccaag aagcctcgta gccatgacta
      781 tcttatcagg catagaataa ccggcatccg cgatattatc tttgtgtatt ctcggattat
      841 aattaccact agttattaag tataaatccc gctcacaacg attatccaaa tcaagagaac
      901 tcttccttgt catttcggac atagaccctt tgtattgtgg tattttatca acccctaatt
      961 cctttgcagc acagagtaaa ctggcagctc cacaagaatt gtcatacatc tgcttcctcg
     1021 ccaaaaaaca accttgttcg tttactctat tctcatttac cctcacatca gtctgatgag
     1081 atatcatata ttctcttggt atagccccct gcaataaaac cccatataac ataacacctg
     1141 tatgaataca tccccgtttg caaggcttcc gttgttccga catcgacgag taattgcagc
     1201 acagtattgc atccagcaat gcgaactact atgagaatta gctgaccggg tccttattcc
     1261 ccgaacgaac tttaaatcca aaacctcata ctcttttccg tcgtcatcac gtccgtcacc
     1321 ataacaacct ttcagcatcc acccttttat taagagttaa tgtccatcag catcagcaat
     1381 taataccgcc tgatactgac atatttttgt cgtatttacg tccataatcg gttattgtga
     1441 tacaggtcac accagcttat ttgctattgt tacaataacc accttggttg agagctgttt
     1501 tttcaacttt gttatcaact cccccaactc cttggtcagt aaggctgccg accgagcaca
     1561 gtgaatagat agtatccgca ggtaccacca tgcttactta atactcagaa ggcgaagtgc
     1621 tctccaatta actctgccgc actatgctta taagctgctt ttcaatgccg tctatcaaca
     1681 tgtccagttc attcaatctg ttgtactggt aatccaacgc agtaatgata tatgcaggta
     1741 ttctttcatc cactccagta acgtttcatg caaagcatta atttacgcgc tacggaactt
     1801 caccagttga ctgagcaaac gatgtgggac cagtactgcc tgcagttctt ctattgttac
     1861 agcgatttct ttaccaaaca gctgaaccac catcaagata aagatagcct gaacattcat
     1921 cgcaccattt tgttaccaat ggcaaaagat ttaacgaacc gtgcctgcat caggcggact
     1981 ttttatccct gctttgttag ctccctaacc ttgtacagag agcctccata agctcccata
     2041 tcaagcagac atggttcgca gttactgaca tacaccagaa aactctgaca gcagactcac
     2101 ttatcctcca cttcactcat atccttattg gtgagatgaa cctgaatgac aggttttgcg
     2161 acataaacgc caaccagtgt atatttcacc tgtagagatt ctacaatctg tagcgttatg
     2221 catctaatgt taggctctat gatgtcagaa atctgcgaga ctctacacct tctgcattta
     2281 cctttattta tcaccatgct cctcagttaa cggagaacgt gttcattgca ttttgtcact
     2341 tacatttcgc ctttggtatc agggggatat aggttccaac gttccgcatg agaacctgaa
     2401 ccggcttcac gccaccttga taccagatgc cgcacaggta gtcaacaagt gtca
//
LOCUS       DADLZM010000269         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLZM010000269 DADLZM010000000
VERSION     DADLZM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.274"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..565
                     /locus_tag="JNY73_004129"
     CDS             <1..565
                     /locus_tag="JNY73_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8049903.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JNY73_004130"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JNY73_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY8049904.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JNY73_004131"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JNY73_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY8049905.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JNY73_004132"
     CDS             2167..2403
                     /locus_tag="JNY73_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY8049906.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          627 a          534 c          626 g          652 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg catgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctatcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DADLZM010000270         2436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLZM010000270 DADLZM010000000
VERSION     DADLZM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.275"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..1334
                     /locus_tag="JNY73_004133"
     CDS             <1..1334
                     /locus_tag="JNY73_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY8049907.1"
                     /translation="VFSITKKHQPYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRI
                     TRTTSGANAGGGNLTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIEE
                     IIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTE
                     TDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPNA
                     TEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAASRGEFVE
                     GISDPNDSKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQTGEDNCPDC
                     GAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELN
                     WKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            1312..1608
                     /locus_tag="JNY73_004134"
     CDS             1312..1608
                     /locus_tag="JNY73_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY8049908.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            1668..>2436
                     /locus_tag="JNY73_004135"
     CDS             1668..>2436
                     /locus_tag="JNY73_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8049909.1"
                     /translation="MGFAGDGKTYTASELAIGLVMLMRQRGIEAGNRPVMFLDTETGS
                     DWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARR
                     RNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKT
                     GIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFKNF
                     LPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEK"
BASE COUNT          740 a          574 c          602 g          520 t
ORIGIN      
        1 cagttttctc tatcacgaaa aaacatcagc cctatgaatt taagaatttc atttcagcct
       61 ggctggatac cgaacacatt gatcgcggtc ttctgacaaa agaatggcga aaagggaatc
      121 gtgtttcacg catcactcgc acgacttccg gtgctaatgc tggcggcggg aacctcaccg
      181 atcgcggcga aggtttcgtc cacgatctga cgtcactgac gcgcgatgta gccactggcg
      241 tactggcccg ttcaatggac gtggacatct ataaccttca tccggcacac gctaaacgca
      301 ttgaggaaat tatcgctgaa aataaaccac ccttttctgt tttccgcgac aaattcatca
      361 ccatgcctgg cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg aaagaagcac
      421 caattgggat cgaggtcatc cccgcacacg tcactgaata tctgaacaaa gtactgactg
      481 aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg
      541 ccccgatgcc gcagcgagta acagaagaag gaaaacagga cgatgaagaa aaaccacaac
      601 catctggcgc aatgacagat gaacaggcaa cggctgaaac agtggaaccg aatgcaactg
      661 aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga
      721 aatatcagga actgcgggca gaactccatg aagcccggaa aaacattccg cccaaaaatc
      781 ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg
      841 acccgaatga ttcgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaaaa
      901 cggaaaatga agtgcgacag actgaagata aacagcacca gaacagcgaa ccggaggagg
      961 gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg gtgatgggag
     1021 acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag gaagaagagc
     1081 cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc gatcagcata
     1141 atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag gtgttgattg
     1201 cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct gcaatccctg
     1261 acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt gatgagcgct
     1321 tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca gcagatcgtt
     1381 cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc tcagcacctg
     1441 tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca ggcaatcagc
     1501 cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg
     1561 gctgaaacca ttgcccacca ccaggttgat attgatttgg aggactaaaa tctatggcgc
     1621 tttttcaacg agcaataaac acacaggcct atctaaaagc aggaattatg gggtttgctg
     1681 gcgacggcaa aacctacaca gcaagcgaac tggctattgg tctggtaatg cttatgcggc
     1741 aacggggaat tgaagctggt aaccgcccgg taatgtttct ggacacagaa acaggctctg
     1801 actgggttaa accccgcttt gatgcggaaa atatcgaatt atttacggca aaaacccgct
     1861 cgtttgttga tctgcttgaa gccattaatg aagcggaaag cagcggttcc gtaatgatca
     1921 tcgactccat cagtcacttc tggaccggat tatgcgacga gtatgccaga cgccgaaacc
     1981 gtaaacgagg actggaattt tcagactggg cgtggctcaa acaggagtgg cgacgcttta
     2041 ccgatcgatt tgttaacagt caggcgcaca ttattatgtg cgggcgtgca ggatatgagt
     2101 atgacttttt cgaaggtgat gatggcaaac gccagttggc aaaaaccggt ataaaaatga
     2161 aagcagaaac agaaaccggt tatgaaccct ccatcctgat ccagatggag aagcaaatga
     2221 acatagaaac cggtcaggtg tggcgcaccg ccagaatact aaaagatcgc tccacccgta
     2281 ttgatggaca ggtattttca aacccaacat ttaaaaactt tcttcctcat atcgagtttc
     2341 ttaacctggg aggtgaacac ctcggcatcg atacctcacg ggataacagt gaattatttg
     2401 ccaacgacgg tacaccgaca tggctaaaag aaaagc
//
LOCUS       DADLZM010000271         2373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLZM010000271 DADLZM010000000
VERSION     DADLZM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.276"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..823)
                     /locus_tag="JNY73_004136"
     CDS             complement(<1..823)
                     /locus_tag="JNY73_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8049910.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="JNY73_004137"
     CDS             complement(1424..1672)
                     /locus_tag="JNY73_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY8049911.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="JNY73_004138"
     CDS             complement(2027..>2373)
                     /locus_tag="JNY73_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8049912.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          439 c          575 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DADLZM010000272         2366 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLZM010000272 DADLZM010000000
VERSION     DADLZM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.277"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..171)
                     /locus_tag="JNY73_004139"
     CDS             complement(<1..171)
                     /locus_tag="JNY73_004139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049913.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..1735
                     /locus_tag="JNY73_004140"
     CDS             308..1735
                     /locus_tag="JNY73_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049914.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNSTKNAAFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLN
                     YGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSA
                     DNVDGVYYRPLQKLINGTWYNVASI"
     gene            1795..2283
                     /locus_tag="JNY73_004141"
     CDS             1795..2283
                     /locus_tag="JNY73_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8049915.1"
                     /translation="MTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEG
                     SS"
BASE COUNT          644 a          554 c          651 g          517 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag
     1321 cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg ggtaatccat ccaggcctgt
     1381 tgttgcagag cttggcgatg attcaggctg gcattttttc agccagagaa acccggataa
     1441 cagcatagtg ttcactgtta acggacaggt aattccgtta aattacggaa acttcgatgc
     1501 ccgctataaa tatcgaacag agggggtaca ggatgtacgg tatggccatg aaatgtatta
     1561 cagccccggc agtaacaccg tttcgtggag attttgcgca ccttcgggac acgggctgtc
     1621 agggatggcg atatcggata ccggccgtaa ctcagcggat aacgttgacg gtgtgtatta
     1681 ccgaccactg caaaaactga ttaatggcac ctggtataac gtagcgagta tttaacaatg
     1741 ttgcatttaa aaaatattac tgcgggtaat ccgaaaacag cagaacaata tcagatgaca
     1801 aaacgatatt cggtcacctg gcttttttca gaagacggaa aaaactggta tgaagagctg
     1861 aagaatttcg ccagcgacac aataaaaata gcttacaccg gagatggtcg cgtggtgtgg
     1921 gtcggtaagg atgtgacagg catcgagcca cgcaatgcca gcgttatcga agttccggat
     1981 attaccgcta accgacggat taccgcgccg ggttactggt tttaccgcaa tgatgaattt
     2041 gtctttgact acagactcaa agcggaagat gagcgtgatg cccttctggc tcaggtcagt
     2101 gcccggacag gggaatggga agaagacctg ctgctggggt taatcagcga cgaagataaa
     2161 gaaaagctga aagcctgtcg tatttacgcg aaatcgctgc aggcgatgga tttcagcacc
     2221 atcactgata aatcctcata caacgccatt gaatggcctg cctctccgga aggttcttcc
     2281 tgatttaatt tatcgcgaga aaaacaatgt ctgtagtgat atcaggtgcg ctgactgatg
     2341 gcgcaggtat ccccatgtcc ggatac
//
LOCUS       DADLZM010000273         2354 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLZM010000273 DADLZM010000000
VERSION     DADLZM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.278"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..329)
                     /locus_tag="JNY73_004142"
     CDS             complement(<1..329)
                     /locus_tag="JNY73_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049916.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
     gene            699..1649
                     /gene="corA"
                     /locus_tag="JNY73_004143"
     CDS             699..1649
                     /gene="corA"
                     /locus_tag="JNY73_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY8049917.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1690..2070)
                     /locus_tag="JNY73_004144"
     CDS             complement(1690..2070)
                     /locus_tag="JNY73_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY8049918.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2084..>2354)
                     /locus_tag="JNY73_004145"
     CDS             complement(2084..>2354)
                     /locus_tag="JNY73_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049919.1"
                     /translation="DGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLA
                     VYIISIITMIAEDFACSGNGSMIFLICYLSTFSMLMLAPTDSSKQ"
BASE COUNT          625 a          547 c          546 g          636 t
ORIGIN      
        1 tgctgaccgt tttcgatgta caaaccgagc ggcgcatagc tttcatcata gatgccgccg
       61 ttcatcgcca tctgcacctg accctgacta ttaatatccg ccagcagagc atgtaacgtt
      121 ccccacgctt cgccattggc tttttgccag tacattttca cccgctctgt ttgaggatta
      181 acggtatacg cctgtacggt cagcgtcgga tctgagagtg cgcaatcatc agcggcaaca
      241 gcaaacaagg gaagtaaggt gagggtgaga aaaatccgtt tgagattcaa ggtgatcatt
      301 cctttaccaa tgagtagccg atacgccatt ataggtcctg gatgtgggat ttttttatcc
      361 tgttagcgac cttgacgagt accaaaaagc gcgaagttca actattgttc tgtggtgttc
      421 tgttgcgtgt tgacggcaaa attttgctgg cgtaacatgc gcgcacgatc actctaagag
      481 gacattcgcc ttggacacac ccagtagata ctggctcact atcctgtcat ccaggatcaa
      541 ctcctaaggc tatccctttt tgctgatagc cttagcggtt gtcagcgacc ttaatttttc
      601 ccgtcgcgct gagtcaggct gtttaatggt ctgaaaccca atttgtttct gtgtgcccac
      661 cgaactgtcc gatattttaa gcattgggag tcccggtcat gctgagcgca tttcaactgg
      721 aaaataaccg actgacccgg ctggaagtcg aagagtcaca accccttgta aatgcagtat
      781 ggattgatct tgtcgaaccg gacgacgacg agcgactgcg cgtacaatct gaacttggcc
      841 agagcctggc aacccgcccg gaactggaag acatcgaagc atcggcacgt ttctttgaag
      901 acgacgacgg cctgcatatt cactccttct tcttctttga agatgcggaa gatcacgccg
      961 gtaactccac tgtggcattt accatccgtg atggtcgtct gtttactctg cgtgagcgtg
     1021 aactgcccgc ttttcgtctg tatcgtatgc gtgcccgtag ccagtcgatg gtagacggta
     1081 acgcctacga gttgctgctg gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa
     1141 ttgaaaatat ctatagcgac ctggagcagt tgagccgggt gattatggaa gggcatcagg
     1201 gcgatgagta cgacgaggcg ctctccacgc tggcggaact ggaagatatc ggctggaaag
     1261 ttcgcctgtg tctgatggat acccagcgcg ctctcaactt cctggtgcgt aaagcgcgtt
     1321 taccgggtgg gcaactggag caggcgcgtg aaatcctgcg cgatatcgaa tccctgctgc
     1381 cgcataacga atccctgttc cagaaggtga acttcctgat gcaggcggca atgggtttta
     1441 tcaacatcga gcagaaccgc atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc
     1501 cgccaacgct ggttgcttcc agctacggga tgaactttga gtttatgcca gaactgaagt
     1561 ggagtttcgg ctaccctggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc
     1621 tgtactttaa gcggaagaac tggttgtaaa aagaaggcgg tggcttagcc tggctaagcc
     1681 acctgttatt caaaggctcc aggtatttaa cccttttacc tctttctcat aaaaccattt
     1741 gttcgtgtta acagcaacat aggctgctac ggcaattccc agaatgttaa cgccaattaa
     1801 tgcaccaaca aatagagata gaatgccaag taatagaact ataattgctt ttttccataa
     1861 gcccagaaca aataaataga tccaggaaca gaagaaagca atgaaattca tttgaatagt
     1921 taatcgttgt cttactttta atgctttaaa tgctgcttta tattctggtg ttgctcccca
     1981 gaatccagga aagccatgtt gatcataaaa attaaatcgg tatttccatt tttcacttaa
     2041 tgaatcatcg ttcatatatt ccttactcat aaatactcca taactattgt tttgatgaat
     2101 cagtaggtgc aagcattagc atactgaaag tggaaagata acaaatcaaa aaaatcatcg
     2161 aaccattgcc tgaacaggca aaatcttcgg ctatcattgt gatgatagag atgatatata
     2221 ctgctaatgt accaaaaaca taaattttta tatatatgaa accactatca cggagtcgtt
     2281 ggcaattcat gttgatgacg agataatgga gtgcgatggt agagactata acaagaaaac
     2341 ctgcttcccc atcg
//
LOCUS       DADLZM010000274         2340 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLZM010000274 DADLZM010000000
VERSION     DADLZM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.279"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..121
                     /locus_tag="JNY73_004146"
     CDS             <1..121
                     /locus_tag="JNY73_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049920.1"
                     /translation="QPKPPAPPAWAMMPPSNSLQLLDETFSVSGTESSATRQH"
     gene            complement(254..766)
                     /locus_tag="JNY73_004147"
     CDS             complement(254..766)
                     /locus_tag="JNY73_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8049921.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            961..1725
                     /locus_tag="JNY73_004148"
     CDS             961..1725
                     /locus_tag="JNY73_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049922.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQTLKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIGGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGECDLNDFYSDIQTDAESGFT"
     gene            1676..>2340
                     /locus_tag="JNY73_004149"
     CDS             1676..>2340
                     /locus_tag="JNY73_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY8049923.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          663 a          521 c          592 g          564 t
ORIGIN      
        1 tcagccgaaa cctccagcgc ctccggcatg ggcaatgatg ccaccgtcga actctctcca
       61 gttgctggac gaaacgttct cggtatccgg gacggaatca tcagcgacca gacagcattg
      121 agaatgcttc aggagtacat caggatccaa tgccttgggg ggtagcggta attttactca
      181 ttatccttca aatcagattc tgttgtcaga ggaatggggg aagctggatt cgcaagagct
      241 ttttatgggc agtttagcta aaaaaaggca acacgaagct aaaactacca acataaagtg
      301 ctgaccagct agcagctgaa atcagactca ctgtatacac tttcactgga tgaagttgca
      361 acattcctgc caccaaagga gcaatgtaac gcagcacggc gataaagcgc gaagtgaaaa
      421 gtattgacac agagttattt tgcaactgaa ggcgaacccg ttctattgta ttggaacggc
      481 tcgatattat acatgcaagt tgagggatat gcccaattat tataccaaga tggtaattaa
      541 cgatagtacc gcaccatgcg ccactcatga ccgtgattcc agcttcccat ggtgaaagag
      601 tcgtcttgct gactgtaatt acagtggcca tcataattga tgctggtggc aatatcgccg
      661 aaatgagtaa agtggatttt gtgaacgcta ttataaaaag aagcccccat aggcgatatg
      721 gatgaagtac aaaatagttc atcaaagcat ttatccactc catcaatgtc tttccttctg
      781 tgaatttttc tttgctattt aaccgtgata cgacttaatt taggcttaaa caccttacat
      841 ttacaaggta ctcctgtggg gggccctgcc acggagcgtc gcaggagtga gtgatgagga
      901 agaattaaag caacctattg tgacaggcaa cgataacagt aaatcacagg aagcataatc
      961 atggcaaaac cggactggga ggccatcgaa tcggcatacc gggccggagt ccttagcctc
     1021 cgtgatatag gcgagaaata cggcgttaca gaaggggcta tcaggaagag ggccaaaaag
     1081 cttggttggg cacgcagtgg cggtacgcag gtttgcaaaa atggtacgca aaaaaggaaa
     1141 gtgcgtacca gcagaaagcc tgccattact ggccttacac aaaaaagtac gcaactaaaa
     1201 acagaatcta caccggatac gaaaccgata cgcggaatgc gtaccgatcc cccgactaac
     1261 ccattccaac ccggtaacca gcagacatta aaacacggtg gttatgcccg tcgccttctg
     1321 ctcaaagatg aggtgataga ggacgctaaa gcgttgacgc tcgaggacga attatttcgc
     1381 cttcgtgcta acaaccttgt cgctgcagag aatattggtc ggtggttggt gtcgctggaa
     1441 gatgctaatg gggaccagga aaggaagatg ctgattgaaa atatcagcgc cgccgagaaa
     1501 gcaatgatgc gcaatacagt tcgtattgag tccatcggtg gcacgcttgc gacggtagga
     1561 aaaatatttg ctgatacagc ctaccgcaag gccgccactg ataaggtgtc tctggaggct
     1621 gatcgtcttc gccgtgatgc aggtattgat gatggcaatg gagagtgtga cctcaatgac
     1681 ttctactctg acatccaaac cgacgctgaa tccggcttta cgtagtttct ggactatgcg
     1741 ggcacgtaac aaagtgcttt atggtggtcg gtcatcgtca aaatcatggg atgccgctgg
     1801 cattgccata tttctgtcga ataaatacac cctgcgtttt tgttgtgccc gtcagatcca
     1861 gaataaaatc gaagagtcgg tgtataccct gctcaaaatt cagatagaca ggtttggtct
     1921 gcggcaccgt ttccgtattc tgaacaacaa aatcattaac cgggttactg gctcggaatt
     1981 tgttttttat ggattatggc gcaacatcga agaaattaag tcactggagg ggatcgatgt
     2041 gttgtggctg gaagaagccc acgcactgac ggaataccag tggaaaattc tggagccaac
     2101 gatccgtaaa gagggttcgg aatgctggtt catattcaac cccggacttg ttactgattt
     2161 cgtctggcgc aacttcgttg ttgatccgcc cgaaggcact ctcatccgca aaattaacta
     2221 tgacgaaaat ccgtttctgt ctgacaccat gcttaaggtt atcgacgcgg cgcgacgccg
     2281 tgatccggat ggttttaaac atgtgtatga gggcgttccg gagtctgatg atgatgcggc
//
LOCUS       DADLZM010000275         2318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLZM010000275 DADLZM010000000
VERSION     DADLZM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.280"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(178..273)
                     /locus_tag="JNY73_004150"
                     /pseudo
     CDS             complement(178..273)
                     /locus_tag="JNY73_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(309..614)
                     /gene="ccdB"
                     /locus_tag="JNY73_004151"
     CDS             complement(309..614)
                     /gene="ccdB"
                     /locus_tag="JNY73_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY8049924.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(616..834)
                     /gene="ccdA"
                     /locus_tag="JNY73_004152"
     CDS             complement(616..834)
                     /gene="ccdA"
                     /locus_tag="JNY73_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY8049925.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(1370..2318)
                     /locus_tag="JNY73_004153"
                     /pseudo
     CDS             complement(1370..2318)
                     /locus_tag="JNY73_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          544 a          628 c          557 g          589 t
ORIGIN      
        1 tgaacttcag cactttttta ttataataag gataaacaaa attggctgta aaaaaacgat
       61 actgtttcag ttaaccgtga atggcctgtt tgcaccgtat tgttcttgtt tttcggttag
      121 ttaaacatcg tacttcatat ttgaacggta agcggctcgt cagaaccgta ttgatattta
      181 ctgagaggtc agatcaactt tccagggcaa cagatcgcgt acccggtttg ccggccagtc
      241 ctggatatgc tcaatgacgt aacgcagcca ttttgtcaac gccacgattt ttgaccctag
      301 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
      361 tggctgagat cagccacttc ttccccgata acgaagatcg gcacactggc catatcagtg
      421 gtcatcatgc gccagctttc atccccgaca tgcaccaccg ggtaaagctc acgggagact
      481 ttatctgaca gcaggcgggc actggccagg gggatcacca tccgtcgtcc gggcgtgtca
      541 ataatgtcac tctgtacatc cacaaacaga cgataacgac tctctctttt ataggcgtaa
      601 accttaaact gcatttcacc agtccctatt ctcatcggca aaagagccgt tcatttcaat
      661 aaaacgggcg atctcagcca tcccctgctg attttcagcc tgccagcgtt ccgcacgcag
      721 acggcgggcc tctttttgca tggccgtatt taccaggcct gagatattca cgtttgcgga
      781 tttaagtaac tgatagctgt cactgtctat agtgacagta atacgctgct tcatgataag
      841 ccctcaaaaa catacttatg atatgtatat gagtatatat taatggtaca aaccatcaat
      901 atccataaaa agtacaccat taattctcta taagttttta atacaatttt ttctgctgcg
      961 atttttttat actataatta gcatacttat aacaccgtaa gtaaattaca tgaaagcagg
     1021 cacagcgtga tccgattttt ctgcgtataa cgattttccg gaagtgtaat acggttgcgc
     1081 cccgggccgg gcaccctttt ctgttgttgt gtacgggccc acagagaatg gtaccggagg
     1141 cgggctcaga agtactgagt cgggtgatcg cgtcaggccg cgaagtactg acacgggtat
     1201 tggtgactgg tgatgaagta ctgccccggg tatgatttca ggctgattca ggttctgatt
     1261 ctctgtcagt ccgcgtggtt gctggcctgg ctgatttcct gttgtcaggc gttcaacaac
     1321 cgactgcagg ttatagcccg gttcacgaac acctgacggc aggtatcact cacccggtga
     1381 aggtaaatcc ctcaagaggc agcaactctg ccagtcgctc ctccggccat tccggcagac
     1441 gcgtcaggac gtccgtcagc caggcatgtg gctccagacc attacgtttt gctgtttcca
     1501 gaaggctcat tatctgtgcc gcccggtttc cggccatcag tgaaccggcg aacagccacg
     1561 ctttccgtcc cataacaacc gggcgaatgg cacgctcgca gatattgttg tccaggggca
     1621 ccgctccgtc acccaggaag cagctcagtt catcgcgccg gttcagaata tagttaatcg
     1681 ccttatttaa tggcccgtcg ggcggacagc acggctcctg ctcttcaagc caggcaaaca
     1741 ggtcattcac tatcggccgg gagtaccgtt atcgccactg ccgtattttt tccacagggc
     1801 gctcacggat gagtttctca atgcggtaaa gacccgcgat ttttctcagc tccacgcctg
     1861 cacggggctc cctgctggcc ttatacagat ttgcgaagcc gcgccctgcg tgactccagc
     1921 aacaggctga tgtgatgccg gggtgattat ccgccagaga tttgtacacg ctgtatccgt
     1981 cactcaccaa cgtaccgcca caccagcact gcagccaggt ctcagggtat ctgagggccc
     2041 gtccggtctg cgagtcaaaa cagacgaccg gcggaccgct tctttcaccg ctgacgtaag
     2101 cacacagcca gccggagcag gatttaccgc ctttccgggt atcaaggagc ctcagggacg
     2161 tttcatctgc atgaataaca tcccgtgtca gaagctcatg gcgtagcagt ttcgccagcg
     2221 gggccagtgc agctccggca acaccgacca tatccgccat tgtactgacg gggagctcca
     2281 cacccattcg ggagaaaatg tgctgctggc gatacagg
//
LOCUS       DADLZM010000276         2302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.281, whole genome shotgun sequence.
ACCESSION   DADLZM010000276 DADLZM010000000
VERSION     DADLZM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.281"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..784)
                     /locus_tag="JNY73_004154"
     CDS             complement(<1..784)
                     /locus_tag="JNY73_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY8049926.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1132..2004
                     /gene="rluF"
                     /locus_tag="JNY73_004155"
     CDS             1132..2004
                     /gene="rluF"
                     /locus_tag="JNY73_004155"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY8049927.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2137..2302)
                     /locus_tag="JNY73_004156"
                     /pseudo
     CDS             complement(2137..2302)
                     /locus_tag="JNY73_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
BASE COUNT          562 a          589 c          577 g          574 t
ORIGIN      
        1 cgctcatatt cgtttcaacc attgcgccgt gaatatcaaa aaagcgcgag caaatatcgc
       61 cagcaacctg gcgggcattc aggtcgtcac tctcttcacc accataaaac gcatgccagt
      121 tagcgccgtc gcgaatggcc ggtgagccaa ttcccaccag ggcgacatcc agattatccc
      181 agtaggcaga gatggtttta aagtgctgag actgcatgat cccattacga attaatgggt
      241 tatccagcag agccggaaaa tccgcgagat gcgattcccc tttcagcttc gctgccgcgc
      301 tgtaggttaa tgtgtttaca tgatagcggc tttcgagttt accggacggt ccgccaataa
      361 ttggtacgca gattaactgc cgcgattgcc ccgcctgcgg caagttttca accagtgcgc
      421 tcaccgcgcg accccaggaa aaaccgacaa tatcgccagg ctccagcaag cgatccagca
      481 gttgcgcgcc gtgtaacccc atcatcgcca gttgagtctc ttcatcctca tcatttcccg
      541 acaccaccac aacgtctttc aggccaaact tttgcttcag ttgctgctcc agccagagat
      601 tttcgttgta gtcatagttg atggcgatgg tgaccattcc ctgatcgcgg cctcgtttaa
      661 gcaagcggct gatggtggtg cggtaaatac ccagttcgcg cgcgatttgc gcctgcgtca
      721 tatcctgttc gtaatagagt tgggcaatct tcacaatcaa acggatatcg tcactgtttt
      781 ccattgccgc ctcctggttc tgcacatttg tgcaaatacg ctttctcacc gcttgatctg
      841 atataacccc atcatgaggg cctttcaaag cgatttttca ctctttgcat ggggagaaat
      901 agcgctttct cttgcacatt tgtttctctg cgtagtagtc atcagcacag gtgtgcagcg
      961 attcgtaata tgttgattat ccttattttg ccaggcaaat ttgcatcgta cttccggaga
     1021 cacttttgtg ataaaaggcg tggcagatca catttaattt cgacgttgcc gcataaccct
     1081 gtttttcccc gctaagaggt acggtatatt ccgcgtttta caggagaatt tatgctgccc
     1141 gactcatcag tccgtttaaa taaatacatc agcgaaagcg gaatttgctc acgccgcgaa
     1201 gcggatcgct atatcgagca aggcaatgtg ttccttaatg gcaagcgagc caccattggc
     1261 gatcaggtga aacccggcga cattgtgaaa gtaaacggtc agttgattga gcctcgggaa
     1321 gccgaagatt tggtacttat cgccctgaac aagcccgtcg gtattgtcag caccaccgaa
     1381 gatggcgagc gcgataacat tgtcgatttc gttaaccaca gcaaacgcgt gttcccgatt
     1441 ggccgcctgg ataaagactc ccaggggctg attttcctca ccaatcacgg cgatctggtg
     1501 aataagatcc tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa
     1561 ccgattaccg atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg
     1621 acgaaaaagt gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag
     1681 gggctgaacc gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg
     1741 gaacgtacgc gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat
     1801 ttaaccgacg atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca
     1861 aaacctaaag cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg
     1921 gaaaaaacgg cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg
     1981 gggcgtaaaa agaagggacg ctgattaacg cctcagactg atgaaaacgt ccaaaaagag
     2041 ctcggacagt cccctcgccc cttcggggag agggttaggg tgaggggaag attgtgtcca
     2101 cacagacgag tgaaaatctc aggcgttgat tgttcattag cgcctgccgc gcgtcgacgt
     2161 atttgctgac cagctaaatg acgcttcagg atccggttta taagcctgct ttttcttcaa
     2221 ttggcgggct tttttcgctt cagcctcacg ctcgaccatt aatttatcga tgtactctct
     2281 ttcttaatgc tgttggtttc gg
//
LOCUS       DADLZM010000277         2280 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLZM010000277 DADLZM010000000
VERSION     DADLZM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.282"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="JNY73_004157"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="JNY73_004157"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY8049928.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYKF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          579 a          564 c          673 g          464 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacgctt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tgactggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactggccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggcgc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgcggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat aagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactctact cagcacatgg
//
LOCUS       DADLZM010000278         2255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLZM010000278 DADLZM010000000
VERSION     DADLZM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.283"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            115..633
                     /locus_tag="JNY73_004158"
     CDS             115..633
                     /locus_tag="JNY73_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY8049929.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            640..816
                     /gene="mbeD"
                     /locus_tag="JNY73_004159"
     CDS             640..816
                     /gene="mbeD"
                     /locus_tag="JNY73_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY8049930.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQV"
     gene            complement(1175..1642)
                     /locus_tag="JNY73_004160"
     CDS             complement(1175..1642)
                     /locus_tag="JNY73_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049931.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(1970..>2255)
                     /locus_tag="JNY73_004161"
     CDS             complement(1970..>2255)
                     /locus_tag="JNY73_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049932.1"
                     /translation="DAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSL
                     REAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR"
BASE COUNT          598 a          447 c          654 g          556 t
ORIGIN      
        1 atgtgattca ggcgctcaca gaggcaggga ttgacgtggt cagggtgacg cgaagcagta
       61 tcagcattgc agacccgaac ggcgggaaga atgtcagact gaaaggagcg ttttatgagc
      121 aatcttttac agacgggtgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
      181 gagaaaatgc tgaacgacga gttcaggaag ctcgaagaat ctgtaagcaa ggctgtgaca
      241 tcaaacgaga cgaaaatcaa agacgctata gccctgttca cagcttcgac agaggaatca
      301 ctgaaaaaac accgggaagg ggtgaaagag gcgatgatgc agcacaggaa ggacgtgtta
      361 aagctggcag ggaatacggg catgatgtta ctggggatgg tcattttcct gtttaccgtg
      421 agtggcggga cgctctggta tcttggcggg atgatacagg cgaacctgga agaaatcagg
      481 aagcagaacg agagtctgga gaagctgaac gcgaagacat ggggcgtgga gtttgtgcag
      541 gacgggaaca ggaaatttct ggtccttcct tacgggaagt cagcagaggt gatttccttt
      601 caggggaagg aatgggtaca tctgaaggag tgacacagag tgacagagct ggaaacacat
      661 ttgctgaacg ccttagagca gctgcaacag gactatatgc agcggctgag cgaatgggag
      721 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
      781 aacgagcggg tcatgcagtt gagtcagcag gtgtagcact tgagcgagca gacagaacgc
      841 ttgagccagt tatacagcga gaactggaga taagagagga acggctgata caggagcgcg
      901 aacatgcgtt atccctggaa cgggagcgtc agccggaaat acaggaacgc acgctggatg
      961 gtccttcgct gggatggtga aaccatgaaa aatggcagct tcagtggatt aggaggggtt
     1021 aatgtggcct gtaccatctg gttgcatagg tattcatgcg gttaaaattt atcaggcgcg
     1081 atcgcggcag tttttcgggt ggtttgttgt catttttatc tgtctgctgc cgtgatcgca
     1141 ccaaacgcgt ttcagtggtg cgggcaataa gatattacgt taaattgtga gagaatggtc
     1201 cccatatact gactttatca tacggatagg gttggtagtg ggtcgattgg gtttcggcat
     1261 tgtttccgtt aatgccaatt gcatttttgt atttgatgca acagaaggcg tgagattctc
     1321 ctgagcctgg aaagtctaag tgtttagaat tgatggcaaa atatcttccg tccgattctt
     1381 gaagtagttg ttgtttggct gtctcccaaa gggtgctgtt acaaacttct tgaatttgta
     1441 gttgttgcag gactcttatt actgcgttgt catgttccaa ttctttaggg gagtgtatta
     1501 ttctctcttt taataagctg tttaccacag gatttagttg ttgtctaaag cacgcagata
     1561 atgccattgc tacacaattt tgttcccgag gaagcatgtt tggcggggta tgccgtgttt
     1621 gatgtgatat gtctattctc atagaaggtc tctggcttat agcatcaaca aatacggcta
     1681 taaaacaaaa ttttcataga gtgattcaag atcttcttga gatctttttt ggcggtgcgt
     1741 aatctgctgc ctgcaaacaa aaaaaccacc gttatcaacg gtggtttgtt tgccggaatc
     1801 tagagctacc aactctttct tccgaaggta actggcttgg tggagcacag ataccaaatg
     1861 ctgtccttct agtgtagccg cagttaggcc accacttcaa gaactcttaa tatctcaatc
     1921 caccttatcc agttaccaat ggatgctgcc agtggcgtta agtcgtgtct taccgggttg
     1981 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2041 acacagccca gcttggagcg aacgacctac accgaactga gataccaaca gcgtgagcta
     2101 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2161 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2221 cctgtcgggt ttcgccacct ctgacttgag cgtcg
//
LOCUS       DADLZM010000279         2156 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.284, whole genome shotgun sequence.
ACCESSION   DADLZM010000279 DADLZM010000000
VERSION     DADLZM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.284"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..79
                     /locus_tag="JNY73_004162"
     CDS             <1..79
                     /locus_tag="JNY73_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HAY8049933.1"
                     /translation="AKQDIEERFDINKTALKILTLAKQK"
     gene            84..1034
                     /gene="virK"
                     /locus_tag="JNY73_004163"
     CDS             84..1034
                     /gene="virK"
                     /locus_tag="JNY73_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY8049934.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1099..2043
                     /gene="lpxM"
                     /locus_tag="JNY73_004164"
                     /gene_synonym="msbB"
     CDS             1099..2043
                     /gene="lpxM"
                     /locus_tag="JNY73_004164"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8049935.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          648 a          408 c          477 g          623 t
ORIGIN      
        1 ggcaaaacag gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac
       61 attagcgaag caaaagtaac aatatgtttt ctataagtaa cttatcattt atcggtttcc
      121 ttaaaaggat tattttttcc tcagattcac taccggggaa gtgggaacac agaaaatttc
      181 ggttcatgta cattttgcga tgttctataa atccggttgt cagtattcga tattattatg
      241 aactgcgttc cttgccgtgc attgaggata ttctggcaat acaccccacg ttgccagcaa
      301 gaattcatcg cccttatttg cataaagggg ggcgcgcctg gacccgtgga caatatattc
      361 ttgagcatta tcgctttgtg caaaatttgc cagaaaaata ttctaaattt ttgtttccac
      421 aaaaatcggt atcattagtc caattcatcg ggaaagatgg agagaatttt gatatccagt
      481 gttctcccag tggttttgac cgggaaggag agttgatgtt gtcattgttt tacaataaaa
      541 cagttattgc ccggttaaca ttttcagtca ttctgactca aaatggacac attgctttta
      601 ttggcggatt acagggagcc cctaaaaata ccggacctga tgttattcgg tgcgcaaccc
      661 gggcctgtta tgggattttc ccaaaacgga ttatttttga agcattttgt gcgttgatga
      721 aagcttgcaa tatctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat
      781 tgagatactg gtatcagaaa cgcaaaacct ttgttgcggt ctatagtgat ttctgggagt
      841 ctgtagcagg aaaaacctgt ggtgactggt acagattacc aacccaggtg attcgaaagc
      901 cgctaagcga tatagccagt aaaaaacgct ctgagtaccg aaaacgatac gctttgctgg
      961 attatatcca tgaaactact atccgctctt tggatgcata tcctgtacac tcagaacatc
     1021 agaatttaaa ttaaggtttg atgtattctc tgaataaaac agtaatgata attttggtag
     1081 aggcattcgc actaaataat gaaaaaatat aaatctgagt ttattcctga atttaagaaa
     1141 aattaccttt cccctgttta ctggtctaca tggttccttt tgggaatgat tgcaggtatt
     1201 tcaatgtttc ccccttcatt cagagatcct gtcttggcaa aaatagggcg ttgggcgggc
     1261 agattgagca aaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccagaa
     1321 aagagtgata cagaacggga aataattgtc gacaaaatgt ttgtcacagc attgcaatcc
     1381 atagtgatga tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc
     1441 tggaaaggcc tcgaaattct tgaggaaatt cgacacaata atagaaatgt gatttttctg
     1501 gttccccatg gctggagcgt ggatattcct gcaatgttgc tggcagctca gggaaaaaaa
     1561 atggccgcca tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattcagta
     1621 cggcgtaaat tcggggggcg cttacatgcc cgggaggatg ggataaaacc atttattcag
     1681 tcagtacgcc agggatactg gggatattac ctcccagatc aggatcatgg tcctgaatac
     1741 agtgaatttg ctgatttttt tgcgacctat aaagcgacat taccaatcat tggacgtctg
     1801 atgaacatca gtcaggctat gattatacca cttttcccgg tttatgatga aaaaaaacat
     1861 ctactgacta ttgaaattcg gccaccaatg gatgcttgca ttgccagcgc ggacaataaa
     1921 acgattgccc gacaaatgaa caaaacagtg gaaattttgg tggggccaca tccggaacag
     1981 tatgtctggg ttttaaaatt gttaaaaaca cgcaaatcaa acgaagcgga cccgtaccct
     2041 tgaaattata aagtacaagt atttccacta gttgtttgta agcgtacagc ctgaaccgtc
     2101 tggtcagaat ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaac
//
LOCUS       DADLZM010000280         2136 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLZM010000280 DADLZM010000000
VERSION     DADLZM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.285"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            14..487
                     /locus_tag="JNY73_004165"
     CDS             14..487
                     /locus_tag="JNY73_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8049936.1"
                     /translation="MRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDELYQV
                     YSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKLALMA
                     MEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNY73_004166"
     CDS             complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNY73_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY8049937.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1111..2124
                     /gene="argF"
                     /locus_tag="JNY73_004167"
     CDS             1111..2124
                     /gene="argF"
                     /locus_tag="JNY73_004167"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY8049938.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          544 a          520 c          532 g          540 t
ORIGIN      
        1 gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat
       61 ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc
      121 gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga
      181 atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga
      241 catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa
      301 aaaactggcc ttaatggcga tggagcaggc tcgagagatg ggcttcaaac gctgctatct
      361 ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca
      421 tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga
      481 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
      541 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      601 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      661 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      721 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      781 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      841 ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      901 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      961 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
     1021 acaaagattt atgctttaga cttgcaaatg aataatcata cacataaatt taattttaat
     1081 tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg
     1141 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
     1201 aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
     1261 atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag
     1321 ggtgctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt
     1381 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
     1441 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1501 aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc
     1561 ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc
     1621 aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1681 gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1741 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1801 accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta
     1861 ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1921 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1981 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga gtctgccgcc
     2041 agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg
     2101 acgctcagta aattaaataa ttaatatctc tataaa
//
LOCUS       DADLZM010000281         2128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLZM010000281 DADLZM010000000
VERSION     DADLZM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.286"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(246..479)
                     /locus_tag="JNY73_004168"
                     /pseudo
     CDS             complement(246..479)
                     /locus_tag="JNY73_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(575..2098)
                     /locus_tag="JNY73_004169"
     CDS             complement(575..2098)
                     /locus_tag="JNY73_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8049939.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          636 a          467 c          451 g          574 t
ORIGIN      
        1 atgacattga tcactttggg gtgactattt tgtttaacaa ataactcttc gacatcaaaa
       61 ttattaatag ttgccatggt aattatttcg gtgatatata aaaacaactg tgataacacc
      121 aaggttgaca actatttgcg caataattcc tttccttcaa tgatccaggc gttcactgcc
      181 agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc tgatccctgt
      241 ggctgacgag tctttctctt aaatcgatta accagattga tcgccagcca aacaagggca
      301 ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt tgcaataagg
      361 atgaccagga gggtgatcag cataaatgca atggaaaaga tgatatagag agtgaaataa
      421 tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact ttcaaacaat
      481 tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt agctgtctat
      541 aattacaccg cactatggcc ttactgatca attttcactc aaccacaacc ttcaccttcg
      601 gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag aaataataac
      661 cctgctgaaa tgtgatatta ttttggttca gatagccaag ttgttctttc gtttcgacac
      721 cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa tccagaattc
      781 gggttgattc tgggtctgcg tttacgcggc caacgaaact gtgatcaatt ttgatataat
      841 caatatgcag gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa tcatccagcg
      901 cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt tcatcaacat
      961 tcaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg gtaaaactgt
     1021 cacggaaatt taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa gcgctaaaat
     1081 taattccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc tgtctggcaa
     1141 cctgattcat caggctttgt gtaagcggaa cgattaatcc ggatttttca gcaagtggaa
     1201 taaatgccgc gggtgaaata tatccaccgt gaggttgttt ccagcgggct aacacctcaa
     1261 ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg acgatctccc
     1321 cattatttat tgcccgtcgc aggatctctt caggcgttgc gcttttattg aaatatctat
     1381 caagcaggaa ggcagcggcg caggcgatta acagaataaa tattaaaatc ccaaagcctt
     1441 tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata gtgaaatgat
     1501 attttgtgga gtgaaccttc tcggtatagg gataatgtcc attaagcgtc atcacatcac
     1561 ccgttggtcc aataatgtca tccgccacgc gtaatacata ccttacccct ttcaagggta
     1621 cattaagcgc cccgcgaata tgttgatcac ttatcgtaac caaaatgcgc gtatctgcaa
     1681 attggctctg atagagcagg ataggtaatc tattaacggt gtcgattgcc ggagccaaca
     1741 gtaaattact gtccgggaca acaggaatac gagacagcag gacccgattc ccaggcaggg
     1801 atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag
     1861 ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg gcaatgttaa
     1921 ccgctgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg ttggcggcat
     1981 atcttgctcc cgccaggtac tctgcttttg cggaatacca gagctggatg ttcaaaatag
     2041 cgacaccggt aataaacaag catatcgcaa cgaggatggg tatgagtgta ttgcgcattt
     2101 gcttgtcccg gccaaagggt ttaataca
//
LOCUS       DADLZM010000282         2118 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLZM010000282 DADLZM010000000
VERSION     DADLZM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.287"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            129..1166
                     /gene="curA"
                     /locus_tag="JNY73_004170"
     CDS             129..1166
                     /gene="curA"
                     /locus_tag="JNY73_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY8049940.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1364..2029
                     /locus_tag="JNY73_004171"
     CDS             1364..2029
                     /locus_tag="JNY73_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8049941.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          583 a          454 c          567 g          514 t
ORIGIN      
        1 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
       61 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      121 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      181 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      241 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      301 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      361 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      421 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      481 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      541 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      601 cgacaggacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtga
      661 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      721 tttgccttga ttaccacgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      781 gtattgatat ctattatgaa aacgtgggcg gcaaggtatt tgatgcagtg ctaccattac
      841 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
      901 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
      961 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1021 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1081 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1141 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1201 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1261 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1321 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1381 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1441 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1501 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1561 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1621 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1681 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcaggcgc aagaaaaggg
     1741 cgacatggaa caaatcatta atgcgaacag gctatttcgc ttagcgattt atcatcgctc
     1801 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1861 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     1921 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     1981 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2041 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2101 taatttcgtc cgggcgca
//
LOCUS       DADLZM010000283         2117 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.288, whole genome shotgun sequence.
ACCESSION   DADLZM010000283 DADLZM010000000
VERSION     DADLZM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.288"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..256)
                     /locus_tag="JNY73_004172"
     CDS             complement(<1..256)
                     /locus_tag="JNY73_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049942.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
     gene            281..520
                     /gene="relB"
                     /locus_tag="JNY73_004173"
     CDS             281..520
                     /gene="relB"
                     /locus_tag="JNY73_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY8049943.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            520..807
                     /gene="relE"
                     /locus_tag="JNY73_004174"
     CDS             520..807
                     /gene="relE"
                     /locus_tag="JNY73_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY8049944.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            879..1034
                     /gene="hokD"
                     /locus_tag="JNY73_004175"
     CDS             879..1034
                     /gene="hokD"
                     /locus_tag="JNY73_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY8049945.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1252..1502
                     /locus_tag="JNY73_004176"
                     /pseudo
     CDS             1252..1502
                     /locus_tag="JNY73_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1569..1847
                     /locus_tag="JNY73_004177"
     CDS             1569..1847
                     /locus_tag="JNY73_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049946.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            1849..>2117
                     /locus_tag="JNY73_004178"
     CDS             1849..>2117
                     /locus_tag="JNY73_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049947.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNAQVIRAAGGAGALSDWLLRH"
BASE COUNT          550 a          483 c          550 g          534 t
ORIGIN      
        1 acatgctaaa gcaaaaaata gatgaataag ttgagttgtg catatgtagc ctgaccgtca
       61 caaagtatat ggtgtctgta ccagtaagat gatggccgga ctctttaaaa aagagctgac
      121 ctgcacaata caggatggac ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca
      181 ccgttcttac gactactttc tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg
      241 atacttgtaa tgacatttgt aattacaaga ggtgtaagac atgggtagca ttaacctgcg
      301 tattgacgat gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc
      361 ttctgaagcg cttcgtctca tgctcgagta tatcgctgac aatgaacgct tgccgttcaa
      421 acagacactc ctgagtgatg aagatgctga acttgtggag atagtgaaag aacggcttcg
      481 taatcctaag ccagtacgtg tgacgctgga tgaactctga tggcgtattt tctggatttt
      541 gacgagcggg cactaaagga atggcgaaag ctgggctcga cggtacgtga acagttgaaa
      601 aagaagctgg ttgaagtact tgagtcaccc cggattgaag caaacaagct ccgtggtatg
      661 cctgattgtt acaagattaa gctccggtct tcaggctatc gccttgtata ccaggttata
      721 gacgagaaag ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc ggaagtatat
      781 agcgaggcgg tcaaacgcat tctctgaacc aaagcatgac atctctgttt cgcaccgaag
      841 gtgacacttc tgctttgcgt tgacaggaga agcaggctat gaagcagcaa aaggcgatgt
      901 taatcgccct gatcgtcatc tgtttaaccg tcatagtgac ggcactggta acgaggaaag
      961 acctctgcga ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc acagcttacg
     1021 aacctgagga gtaagagacc cggcgagtga gaaatccctc gccacctctg atgtggcagg
     1081 catcctcaac gcacccgcac ttaacccgct tcggcgggtt tttgttttta ttttcaacgc
     1141 atttgaagtt ctggatggtg ccggaataga atcaaaaata cttaagtagc gcgcagggat
     1201 aaaagggatg gaccccgaac aggggagtgc tacttatctg gaaggattct gatgatgaac
     1261 atcgaagaac tgcgtaaaat tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat
     1321 ggaaatattg ttagtcatta ccgcattgtg tgtttgcaaa aaatggggct gcgttaatta
     1381 attttgtgga tgcccgagtg acggacggat ttatcttgcg cgacggtgag tttgtcactt
     1441 cattacaggc attgaaagag accggaataa aagctggctt ttctgctttt tcagaagaat
     1501 aaactcatct acaatcttgc gcggggctga actcccgctg agtaacaccg tgccaccgga
     1561 gaaaaccgat ggcacgcaac gcaaaatatt acaatcatga taattcgacc gttcttgccc
     1621 acacgcacga gcggtattct cacgcattta agtcagactg gtaccagcat ccctcatgca
     1681 ctgaagaaca ggccgaatgg ctcattcagt gttaccgcag gcgcggatgc gaggttaaaa
     1741 aagcccttag cctcgactac cgtcactgga taatctccgt caggctccct tactccgaac
     1801 agccaccgcg tccgtcccgc acattccagc aacggatctg gaggtaacgt gcgggtatta
     1861 cttcgacctg ttctggttcc ggaactcggg ctggtggtcc ttaagcccgg tcgtgaatcc
     1921 atgccggtat tccacaatac ccggttactg gtggagccgg aaccgaaaag catgcgtaat
     1981 ctgccgtccg gggtcgttcc tgccgttcgc cagccgctgg tggaagacaa aacattgctg
     2041 ccgtttttca gtaacgcaca ggtgattcgt gctgctggtg gtgctggtgc attgtctgac
     2101 tggctgttgc gccatat
//
LOCUS       DADLZM010000284         2109 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLZM010000284 DADLZM010000000
VERSION     DADLZM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.289"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..537
                     /gene="traX"
                     /locus_tag="JNY73_004179"
     CDS             <1..537
                     /gene="traX"
                     /locus_tag="JNY73_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY8049948.1"
                     /translation="FALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAYYLAGFPWYEG
                     NILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLMLAVSHRLYR
                     AEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKSLPRFWPGD
                     FFPVFYACHLAVLGVLAL"
     gene            592..1152
                     /locus_tag="JNY73_004180"
     CDS             592..1152
                     /locus_tag="JNY73_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY8049949.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            1284..1496
                     /locus_tag="JNY73_004181"
     CDS             1284..1496
                     /locus_tag="JNY73_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY8049950.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            1679..>2109
                     /locus_tag="JNY73_004182"
     CDS             1679..>2109
                     /locus_tag="JNY73_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY8049951.1"
                     /translation="MCCDIQDYLKYRCHLSDMENNMRKYIPLVLFIFSWPVLCADIHG
                     RVVRVLDGDTIEVMDSRKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYF
                     QRDRYGRILGQVYAPDGMNVNQFMVRAGAAWVYEQYNTDPVL"
BASE COUNT          511 a          469 c          648 g          481 t
ORIGIN      
        1 tttgccctgg tgtgggggct gaatctgtcc cgtcatgcgc atatccggca accggccatt
       61 aaccggctgt ggggatgggg gattattgcc cagttcgcat attatctggc aggctttccc
      121 tggtatgagg ggaatatcct gtttgccttt gcagtggcgg cacaggtgct gacgtggtgt
      181 gaaacgcgca gtgggtggcg tacagcagcg gccattcttc tgatggccct gtgggggcct
      241 ttgtccggca ccagttacgg cattgccggg ctgctgatgc tggcagtcag ccaccggctg
      301 taccgggcgg aagacagagc ggaacgtctg gcgctggtcg cctgtctgct ggctgttatt
      361 ccggcgctta accttgccac cagtgatgca gcggcggtgg ctggtcttgt gatgacggtg
      421 ctgaccgttg gtctggtgtc gtgtgcagga aaatcattac cccgtttctg gcccggggat
      481 tttttcccgg tattctacgc ctgtcatctg gctgtgctgg gcgttctggc gctgtgacgg
      541 gtgtggtatc tttgaccgta acaggatgat tcgtcagagg cagaacacag catgacagag
      601 cagaagcgac cggtactgac actgaagcga aaaacggaag gggagacgcc tgtccgcagg
      661 cggaaaacca tcatcaatgt caccacgcca ccaaagtgga aggtgaaaaa gcagaagctg
      721 gccgagaaag cagcccggga agcggagctg gcggcaaaaa aagcgcaggc cagacaggcg
      781 ctgtccattt atctgaacct gccctcgctg gatgaggccg tgaacaccct gaaaccctgg
      841 tggccgggat tatttgacgg tgaaacgccc cggcttctgg cctgcggtat ccgggacgtg
      901 ttactggaag acgtggcgca gcggaatatc ccgctctcgc ataaaaaact gcgcagggcg
      961 ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca tgaaagccgg tgcctgccgg
     1021 tatgatacgg aagggtatgt gacggagcat atttctcagg aggaggaagc gtatgcggca
     1081 gcgcgtctgg ataaaatccg ccgccagaac aggataaagg cagagcttca ggccgtgctt
     1141 gatgagaaat aaaaaaaagc ctccatcaga gaggaggcag ggaaataagg cttaaaagga
     1201 aataacttcc gtacccgaaa ggaagccaat tcagtagatt atttaacacc ataatactga
     1261 cgggttaaag aggagtgata aagatgaatg gattcagaaa cagttcacgg aacggtcagg
     1321 tctggcgctg gcagcgtgcc ggaagccggg ccgttattct ggaggtcagt ggacgctgga
     1381 tggaagcggc agaagcatgg cgacgggctg ccggtgttgc gccccggaca gactggcagc
     1441 agtttgcccg aaaaagggct gaacactgcc accggcgcag ccggggaagg gtgtaacgaa
     1501 aaaagcagcc ttcattactc cagggattca gaaggctgaa taatgcatga gatttgcttt
     1561 ttttaatatc cggtcgggca gtatgaccgg acagttctga gtagcacaga gttttttccg
     1621 tattaagtcg tgattttata ttttgtgatt aatttcacaa aataaggtgt tattcagtgt
     1681 gtgctgcgat attcaggatt acctgaaata caggtgccat ttatctgata tggagaataa
     1741 catgaggaaa tatattccac tggtattatt tatcttttca tggccggtat tgtgtgcaga
     1801 tattcatgga cgggttgttc gtgtactgga cggtgatacc atcgaggtca tggactcacg
     1861 gaaggcagtg cggatcaggc tggtaaatat tgatgcaccg gaaaagaaac aggattatgg
     1921 ccggtggtca acggacatga tgaaatcact ggttgcagga aaaacggtta ccgtcacata
     1981 ttttcagcga gatcgttatg gtcgtattct cgggcaggtt tatgcaccag acggaatgaa
     2041 tgttaatcag tttatggtcc gggccggggc tgcctgggta tatgagcagt acaacacaga
     2101 tcccgtgct
//
LOCUS       DADLZM010000285         2088 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLZM010000285 DADLZM010000000
VERSION     DADLZM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.290"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(60..1025)
                     /locus_tag="JNY73_004183"
     CDS             complement(60..1025)
                     /locus_tag="JNY73_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAY8049952.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1878..>2088)
                     /locus_tag="JNY73_004184"
     CDS             complement(1878..>2088)
                     /locus_tag="JNY73_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049953.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          575 a          548 c          437 g          528 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gtgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac ccccaaaaca agcacccccg aacggcttac cccaaccttt
     1381 tccacgcacc cgatcaccaa aacagggaat aaaaacaaaa ccctgtctta caggtggatg
     1441 gcctcacttc gtgaaaccat ccaccccgca taagtttttt catcggtggt gtcgtcaatc
     1501 actaaatcgg aaccccaaag gggaccccca cgatttagca tttgacgaga ttaactctcg
     1561 gagatggcat catgctttta acggacttta aaaaacggtg aacggatgcg gattttaatc
     1621 cgtgtctgtg aggcgttgag caacgagcaa agcgaggcgg tagaaagaga gccagcggca
     1681 ggctcaggac gataatccgg ggaaaatgta ccactcgcag agtgttaaca tttgacatcg
     1741 gattgtctat cctgcattgg tgtcgagttg gcaaggctga ggataatgct gaggataatg
     1801 caggatagac agagcactag agattgcatg caatctcgta cctatcacct cagcctgccg
     1861 ctggctcttt tccagaacta agaatgcgtt cttaaaaatc caaacttgat accgtttaaa
     1921 tctattttca caaaaagagt ttttaccctc tcgtttgttt tctgaatata taaaattaat
     1981 tcatcctcaa aaccttcgtg cggatgaaaa ccaaatgtgc caaaacttaa tccatcaata
     2041 agcaaaataa ggtttttaaa cctgtcagga atcactgctt cacttctt
//
LOCUS       DADLZM010000286         2087 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.291, whole genome shotgun sequence.
ACCESSION   DADLZM010000286 DADLZM010000000
VERSION     DADLZM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.291"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..799
                     /locus_tag="JNY73_004185"
                     /pseudo
     CDS             1..799
                     /locus_tag="JNY73_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1238..2075
                     /locus_tag="JNY73_004186"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="JNY73_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          505 c          631 g          436 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc ggtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggt cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acttggtgat gaagtctctt gatcaggagc tctgatcaag agacagc
//
LOCUS       DADLZM010000287         2072 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLZM010000287 DADLZM010000000
VERSION     DADLZM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.292"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..2057
                     /locus_tag="JNY73_004187"
     CDS             <1..2057
                     /locus_tag="JNY73_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8049954.1"
                     /translation="SMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQ
                     TRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPRLEGAWAPEH
                     SVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTH
                     LNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWENNPDALALV
                     DNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRIRFT
                     KSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAIIAHGAQ
                     GASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKG
                     TAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKY
                     PQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNG
                     ESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAG
                     NGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQER
                     DRAAITEREASLPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTPG
                     GD"
BASE COUNT          462 a          540 c          723 g          347 t
ORIGIN      
        1 gctctatggt gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg
       61 gtcgggctgt ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt
      121 tccgtctcca gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc
      181 agacgccgga actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc
      241 tgtccgggct tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac
      301 cggagcactc cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc
      361 agaaggcgat gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca
      421 ttgtccgcga ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc
      481 acctgaatga ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg
      541 gagaactggg gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg
      601 gggagctgcg tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata
      661 atgtgtatca ccggattgcc ggtatcagca aggatgacgg gctgataacc ctgcaggatg
      721 cggaaggtaa cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt
      781 acaccccgga caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc
      841 gggagcgcgg ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg
      901 tcacactttc tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc
      961 agcatattga cctggcctat gccatcatcg cccacggtgc acagggggcg agtgaaacct
     1021 ttgccatcgc gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag
     1081 cctacgtggc cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg
     1141 gctggacgga tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc
     1201 cgaaaccgga ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc
     1261 gggacgtggc ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc
     1321 ctgcacggtt tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt
     1381 ttgaccgtaa cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa
     1441 acgggctgcg gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg
     1501 tggccctgca gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg
     1561 tccggattgc ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc
     1621 gtccgtggaa tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca
     1681 gcgtccagcc gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc
     1741 aggctgaaga ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa
     1801 tggcagagaa caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg
     1861 ccggtcagga gcgtgaccgg gctgccataa ctgaacggga agcctcgctg ccggagagtg
     1921 tactgcgtga accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc
     1981 tgcaggagcg tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa
     2041 ccccgggcgg agactgatac aggaagacaa ac
//
LOCUS       DADLZM010000288         2059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLZM010000288 DADLZM010000000
VERSION     DADLZM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.293"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..1501)
                     /gene="pykF"
                     /locus_tag="JNY73_004188"
     CDS             complement(89..1501)
                     /gene="pykF"
                     /locus_tag="JNY73_004188"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY8049955.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          523 a          457 c          497 g          582 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac
      361 cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac
      421 cacggcatac cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc
      481 tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg
      541 ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg
      601 cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca
      661 gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct
      721 tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca
      781 tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga
      841 ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga
      901 taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac
      961 caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag
     1021 gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt
     1081 tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt
     1141 tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc
     1201 cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt
     1261 taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg
     1321 cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg
     1381 catagtcacc atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag
     1441 ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca
     1501 tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc
     1561 cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc
     1621 gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg
     1681 agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt
     1741 atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg
     1801 ttagacaata aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt
     1861 cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc
     1921 cggtgtataa atatatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg
     1981 tctgattttg tttgattttt gtttaattgg ttagattgat atatgctgat agttggcatg
     2041 ataaataggt ataaagaat
//
LOCUS       DADLZM010000289         2048 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLZM010000289 DADLZM010000000
VERSION     DADLZM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.294"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(59..328)
                     /locus_tag="JNY73_004189"
     CDS             complement(59..328)
                     /locus_tag="JNY73_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8049956.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            385..1053
                     /locus_tag="JNY73_004190"
     CDS             385..1053
                     /locus_tag="JNY73_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8049957.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1369..>2048
                     /locus_tag="JNY73_004191"
     CDS             1369..>2048
                     /locus_tag="JNY73_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8049958.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          615 a          448 c          382 g          603 t
ORIGIN      
        1 ttaccagcca accgcagcac gtcctgcata cgtcgtgtct gcggtttttc tttttcgctt
       61 acattgtgtc tggttcttcc ggccactcaa tatcaggtgc agttgatgta tcaacacggt
      121 tcagcaacac ccgatacttc ttccaggctt ccagcaacga gttttcttcc tccgttgcga
      181 tctccagatc tacagcatcc tgaagtggcg caatatgctc actgaatccc tggatgtaga
      241 actgtgtggt gacggtcttc cagccattcg gctcctgctg tatcgaagca taccaggcta
      301 tttcaatatc gctatgctgc ggcagcattt aaccccttgt aattcatcgc cataattgat
      361 ttaattcaca aataaaacta taatatggtg aaattaatga aaaaaaacac agatgatggg
      421 gctaaaattt acacaccact taccctaaag ctttatgact ggtgggtttt gggagtatca
      481 aatcggcttg catggggatg tcctacaaag gaacaccttc ttccacactt tctggaacat
      541 ttaggtaaca accatctgga tattggtgtt ggaactgggt tttaccttac tcacgtacct
      601 gagagtagtc tgatatcttt aatggatttg aacgaagcta gcctgaacgc ggcatctaca
      661 agggctgggg aatcaaaaat taaacataaa attagccatg atgtttttga accttatcct
      721 gcggcgttac atggtcaatt tgattccatt tccatgtttt accttcttca ctgcctgcct
      781 ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt aactgatgat
      841 ggaactctat acggagccac aattcttggc gatggagttg tgcacaatag cttcggtcaa
      901 aaactgatgc gcatttacaa tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa
      961 ggcttaacac atatactctc agagcatttc gagaatgtta aaaccaaggt tcaaggtact
     1021 gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg ttcttgctta
     1081 agacgtgcta cggtttcctt tacgatttgg ggttgcgact ttacctctat tgataatgca
     1141 ttccggcaga acgttcaaat atgaacgtac gatatttaac taaccgaaaa acaaaatata
     1201 gcacaggcga gacatttacc atctaagaaa atagtatcgt ttttttacag ttaaatcaac
     1261 atcacttcct taaaatgaaa acaataaatt tcaatcctga aacattttat ttcagcttat
     1321 tattccccag tatcacattt tatcaggata tccttctgcg aggttattat gcttcctgta
     1381 aataatcccc ccctatccac tggaaacgtc tctttttaca gaactacatc aatcgacaat
     1441 gttcacaata attatctctc cgaatgggtt gaatggacta aaaacagcat ttccggagaa
     1501 aacagggaaa ctgcttttac ccggctccaa ttatgtctgg agaacagtga aacatcgttg
     1561 gacttatctt gtttaggtct cagatctcta ccacgattgc ctgacaatct tgatgaaatt
     1621 aatgtaagca ataaccaact atcaatgctc cccgagctac caagggcatt gaaagagctg
     1681 aatgcaagca gtaatcaatt atctgcactt cctgaattac cagtgtcgct ggaatatata
     1741 aatgtgagtg ataaccattt gttcgcactt cctgaattac ctgcgtcact agaatatatt
     1801 aatgtaagtg acaatcacct gtctgtactt ccgaggttac caatgtcatt ggaattactt
     1861 gatgcagcca gaaatgcttt ggaagtaata ccagagtttc cagaaagaga tgatcatatt
     1921 ataagaatat tctggcttaa tcagaaccgg atcacggcaa ttccggaaag catacttggc
     1981 ctcagttctg atagcgttgt caatcttaga gaaaatcaac tatctcccag aataatgcaa
     2041 actttgtt
//
LOCUS       DADLZM010000290         2041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLZM010000290 DADLZM010000000
VERSION     DADLZM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.295"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(220..384)
                     /locus_tag="JNY73_004192"
     CDS             complement(220..384)
                     /locus_tag="JNY73_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049959.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1285..1569
                     /locus_tag="JNY73_004193"
     CDS             1285..1569
                     /locus_tag="JNY73_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8049960.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1557..>2041
                     /locus_tag="JNY73_004194"
     CDS             1557..>2041
                     /locus_tag="JNY73_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8049961.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          528 a          427 c          583 g          503 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaaggaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg
      481 aaaatgtgct atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg
      541 gcatccggca tataggcagc atgaacgcaa cacaagctct cgtacggaac gtgggaacct
      601 gtcgtctcga tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga
      661 tgcgagacac aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga
      721 gcgaaggggg tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc
      781 atgagtcagc caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg
      841 gtcaaagcta atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt
      901 accaatcgaa gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg
      961 cccgcaaaca caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa
     1021 ttcgtcacga ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg
     1081 ttcaacgaca agagccgtat gacgcgagag tgtcacgtac ggttccgtgg gagcgtaggg
     1141 gggcggtgcc cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa
     1201 atgccgctga cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt
     1261 caatatgcta ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag
     1321 ctcaatctca gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt
     1381 cataagtcgc gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt
     1441 ctcgaccggc gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt
     1501 gatgctccgg ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg
     1561 gatgtgtaac tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagcg
     1621 gtgaagctgt acttgatgac tggttaaagc agaaggggct aaaaaatcag gcaatcggag
     1681 ccacccggac ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac
     1741 tggccactgg tagtatcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag
     1801 accccattcc tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag
     1861 ggcttggcgc ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata
     1921 tcggtgtgcg tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc
     1981 atcatggttt taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat
     2041 a
//
LOCUS       DADLZM010000291         2032 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLZM010000291 DADLZM010000000
VERSION     DADLZM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.296"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..362)
                     /locus_tag="JNY73_004195"
     CDS             complement(<1..362)
                     /locus_tag="JNY73_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8049962.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQPVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JNY73_004196"
     CDS             complement(429..1643)
                     /locus_tag="JNY73_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY8049963.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVMMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          492 c          413 g          493 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactggc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcat gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgactactac catacaagag agaaaaagcg attattcaac cgtgttcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DADLZM010000292         1976 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLZM010000292 DADLZM010000000
VERSION     DADLZM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.297"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..537
                     /gene="traX"
                     /locus_tag="JNY73_004197"
     CDS             <1..537
                     /gene="traX"
                     /locus_tag="JNY73_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY8049964.1"
                     /translation="FALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAYYLAGFPWYEG
                     NILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLMLAVSNRLYR
                     AEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLCAGKSLPRFWPGD
                     FFPTFYACHLAVLGVLAL"
     gene            592..1152
                     /locus_tag="JNY73_004198"
     CDS             592..1152
                     /locus_tag="JNY73_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY8049965.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            1283..1495
                     /locus_tag="JNY73_004199"
     CDS             1283..1495
                     /locus_tag="JNY73_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY8049966.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            1679..>1976
                     /locus_tag="JNY73_004200"
     CDS             1679..>1976
                     /locus_tag="JNY73_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAY8049967.1"
                     /translation="MLHYSGGLKYRWHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT          478 a          448 c          607 g          443 t
ORIGIN      
        1 tttgccctgg tgtgggggct gaatctgtcc cgtcatgcgc atatccggca accggccatt
       61 aaccggctgt ggggatgggg aattattgcc cagttcgcat attatctggc aggctttcct
      121 tggtatgagg ggaatatcct gtttgccttt gcagtggcgg cacaggtgct gacgtggtgt
      181 gaaacgcgca gtgggtggcg tacagcagcg gccatactcc tgatggccct gtgggggcct
      241 ttgtccggca ccagttacgg tattgccggg ttgctgatgc tggcagtcag caaccggctg
      301 taccgggcgg aagacagagc ggaacgtctg gcgctggtcg cctgcctgct ggctgttatt
      361 ccggcgctta accttgccac cagtgatgca gcggcggtgg ccggtcttgt gatgacggtg
      421 ctgaccgttg gtctggtgtt gtgtgccgga aaatcattac cccgtttctg gcccggagac
      481 tttttcccga cgttctatgc ctgtcatctg gctgtgctgg gcgttctggc gctgtgacgg
      541 gtgtggtatc tttggccgca agaggatgat tcgtcagagg cagaacacag catgatagag
      601 cagaagcgac cggtactgac actgaagcgg aagacagagg gaacagcgcc tgtccgcagc
      661 cggaaaacca tcatcaatgt caccacgcca ccaaagtgga aggtgaaaaa gcagaagctg
      721 gccgagaaag cggcccgtga agcagagctg gcggcaaaaa aagcgcaggc cagacaggcg
      781 ctgtccattt atctgaacct gcccacgctg gatgaggctg tgaataccct gaagccctgg
      841 tggcctggat tatttgacgg tgacacgccc cggcttctgg cctgcggtat ccgggacgtg
      901 ttactggaag acgtggcgca gcggaatatc ccgctctcgc ataaaaaact gcgcagggcg
      961 ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca tgaaagccgg tgcctgccgg
     1021 tatgacacgg aagggtatgt gacggagcat atttctcagg aggaggaagc gtatgcggga
     1081 gcgcgtctgg cgaaaattcg tcgccagaac cggataaagg cagaacttca ggccgtgctt
     1141 gatgagaaat aaaaaaagcc tcccccagag agaggaggca gggaaataag gcttaaaagg
     1201 aaataacttc cgcttccgaa aggaagccag tccactgaat tatttatcac aataatactg
     1261 acaactaaag ggggacaaga aaatgaatgg attcagaaac agttcacgga acggccaggt
     1321 ctggcgttac cagcgtgccg gcgggcgggc cgttattctg gaagtcagtg gacgctggat
     1381 ggaagcggca gaagcatggc gacgggctgc ctgtgttgcg cctaggacag actggcagca
     1441 gtttgcccgg aaaagggctg aacactgcca ccggcgctgc cggggaaggg tgtaacgaaa
     1501 aaagcagcct tcattactct aggagtcaga aggctgaaag gagtttatga gatttgcttc
     1561 ttaatatccg gtcgggcagt atgaccggac agttctgagt agcacagagt cttttccgca
     1621 ttaagtcgat tttttatatt ttgtgattaa tttcacaaaa taaggtgtta ttcaatgtgt
     1681 gctgcactat tcagggggtc tgaaatacag gtggcattta tctgatatgg agaataacat
     1741 gaggaaatat attccactgg tattatttat cttttcatgg ccggtattgt ctgcagatat
     1801 tcatggacgg gttgttcgtg tactggacgg tgataccatc gaggtcatgg actcactgaa
     1861 ggcggtgcgg atcaggctgg taaatattga tgcaccggaa aagaaacagg attatggccg
     1921 gtggtcaaca gacatgatga aatcactggt cgcaggaaaa acggttaccg tcacat
//
LOCUS       DADLZM010000293         1969 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLZM010000293 DADLZM010000000
VERSION     DADLZM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.298"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            338..591
                     /locus_tag="JNY73_004201"
                     /pseudo
     CDS             338..591
                     /locus_tag="JNY73_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            748..>1969
                     /locus_tag="JNY73_004202"
     CDS             748..>1969
                     /locus_tag="JNY73_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8049968.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAA"
BASE COUNT          520 a          389 c          447 g          613 t
ORIGIN      
        1 ggcaacatac gttatatctg cgacccagac ctgattaggg gggttgggga gcaatggaac
       61 agtaaacgcc gttaagaagt caatttaaat agccaaaaat taactagcca atttacattg
      121 caggttgtaa tttttaacca ccttttttac gatagccaaa ttacctatac ccatcccggc
      181 cctgccaagc tatacccgct atcggcgatg aaaatgcttt tgtgcgcggt cggagtggtc
      241 ccgacgaggg ttttacccga agtcggggcg tatctccgcg ttagcgggcc gtgagggccg
      301 cttacgagcg tgtatgagag acgtacagtg gttttgtccg ctcacaccag tacatacagg
      361 tacggaaata aaaccagtcg aaatgccggt aacgacgatt actcctgtct ctgatgccgg
      421 tggactacaa gattttattt actggcgtcc tgatgcggac aggactggta tggaacctgt
      481 ttatgtgata cttaatgatc ctctggattc cgggcgattt acgcgtaaac aactaaataa
      541 aaatatctta aacatgcaag taattttggt attctcgata caagaataaa tagtagtaac
      601 tactatagtt gcaaataatg ttaaaaacat ctctatgatc atctttacat ttttcatgat
      661 tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg tgataatgca atactttgag
      721 tgattatata atgataagta aacaggtttg cgacaattgg ttatcagtat tacagaagga
      781 ttaaatatgt cattattcac agaacctaag gaaattgaaa gattaccctc tgaagagatt
      841 gaacgattat atcccgtcct gcgatacagg gtctttatca gcattttcct cggatatatg
      901 gggtattatt ttgtccgaaa caccaccagt gtgttatccg gtgtgctgca catgtctgca
      961 accgagatcg gcattatctc ctgtgccggt tttttatcct atggtatatc aaagtttgtt
     1021 tccggactta tttctgacag aagtaattca aaagtatttt tgtctcttgg tttattcctg
     1081 tccggactgg taaattttct gataggatat attcccggga tcatcacatc cgttacgtta
     1141 ttttcaacaa tgtatttact taacggctgg atccaaggta tgggatatcc tcctggtgcc
     1201 aaaacactgg tgttctggta tgaacatcgg gaaaggatca cctgggccac actgtggaat
     1261 ttatcgcaca atgtcggggg agctctggcc cccgttctga ttggtttcag ttttggtttt
     1321 ttcggcgaca gtgctctgga gcatgcccgg gccgcattta tcttcccagg agtactgtgt
     1381 atggcaatga gtgtgctgat ttactttatt caggtggaca gacctgtatc agtggggctt
     1441 cctcccattg aagaatggaa aggtaacgtc gtttcacacc cggctaaagg ccgtgaacaa
     1501 ggtccgagac tctccattcc tgatatcatc agaaaacata tcatccgaaa taataaactc
     1561 atttactgct gtatctatgg ttcgtttgtt tatattctga gatacggtat tgtttcctgg
     1621 gctcctaagt ttttaagtga cagtctggat gtcggtggta aggatatggg aaaactggca
     1681 tcaatggggg ggttttctgt ttttgaaatc ggtggtgtgg ctggtatgct tcttgccgga
     1741 tacctgtctg tcagactgtt cagaaactca aaaccattaa caaatacttt atttctggca
     1801 cttaccatta ttttattaat cgcgtactgg tacgtgccat caggcaatga atatctctgg
     1861 ctaaactata caatccttat tctgttgggg ctggcagtgt atggtccggt gatgtttatt
     1921 ggtctttatt caatggagct tgttcctaaa gaagctgccg gagccgcat
//
LOCUS       DADLZM010000294         1895 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLZM010000294 DADLZM010000000
VERSION     DADLZM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.299"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..219)
                     /locus_tag="JNY73_004203"
     CDS             complement(<1..219)
                     /locus_tag="JNY73_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8049969.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNL"
     gene            525..1307
                     /locus_tag="JNY73_004204"
     CDS             525..1307
                     /locus_tag="JNY73_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY8049970.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(1309..1407)
                     /locus_tag="JNY73_004205"
     CDS             complement(1309..1407)
                     /locus_tag="JNY73_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY8049971.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
     gene            1783..>1895
                     /locus_tag="JNY73_004206"
     CDS             1783..>1895
                     /locus_tag="JNY73_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8049972.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTH"
BASE COUNT          531 a          451 c          386 g          527 t
ORIGIN      
        1 aagattacga atagtattat ccgcaaaaat atcatcacct actttagtca gcatcaggac
       61 ttttgaattc aatttagccg ccgccaccgc ttgattagca cctttcccac cacatccgat
      121 tttgaaggca ggtgcttcca gagtttctcc ttctttaggc atctgattag tgtaagtaat
      181 gagatccacc atattggaac caataactgc aatgtccatt tcactacctc ttataaactt
      241 tcgcataaca atggtattta aataacatta gcatgttact tttgcatcat ttgtgactga
      301 gatcgcgatt agcacatcaa cccgatgttt atttaataga cttccagtct catcactcag
      361 gccaacacta tctaatcata agcaacctaa caagattagt gcccaaaatt cagcagccta
      421 tacccttttc atttcaaagg ggcggtcgta tagtatggta atgaaaacaa tgtttactaa
      481 cgccaaaatg ttatttttat aacattctta cggagagaga gttgatggaa acgaagcaaa
      541 aagagcgtat ccgacgtttg atggaactgc ttaagaaaac cgacagaatc catttgaaag
      601 acgcagcgcg aatgctggaa gtttctgtaa tgactattcg tcgcgatctc catcaggaag
      661 atgaacctct gccactgacc ctactgggtg gctatattgt aatggtgaat aaacccgcgc
      721 catccatgcc agtaatccat gacgttccaa aaaatcatcg tgatgactta cctattgcaa
      781 ttctggctgc cggaatggtt aatgaaaatg atctgatctt ctttgataat ggccaggaga
      841 taccactcgt tataagcatg atcccggatg caatcacctt caccggcatc tgttactcac
      901 atcgcgtctt tgttgcgttg aatgaaaagc ctaatgtaac agcaatactt tgtggtggta
      961 cgtatcgtgc cagaagtgat gctttttacg atgccagtaa ctcttcgcca ttagactctc
     1021 tcaatccgcg aaaaatattt atttccgcca gcggtgtgca taatcacttt ggcgtcagct
     1081 ggtttaaccc tgaagatctt gccactaagc gtaaagcgat gaaccgtgga ctacggaaaa
     1141 ttttgctcgc ccgccacgcg ttgttcgatg aagtggcctc tgccagcctc gcaccgatct
     1201 ctgcatttga cgttctgatt agcgatcgtc cgttaccggc agattatgtt acgcactgcc
     1261 agaatggttc tgtaaagatc attacacctg attcagaaga cgaatgactt actgaaaaaa
     1321 caccacaatc ttgttaaaca tcgtcgggtt ggactgatta cgttgcactt tcaccacata
     1381 ttccagctta tctatttggc ttatcaccta ctccagacgc tggtcatcct tgaccagtag
     1441 ccagatatgg cttttgtcgc agtcctgaat cggcaggcag agaatgtctt caacgttaag
     1501 agcgcgacgg gcaaaagccc acaaaggtga gtcattacac ccggatggta aacaggcact
     1561 tccttgttgt agaagccgtt atattgtcca taatgtctgg gtttttaatg catactgggt
     1621 aacaaccgga cgaatcttcg aatatcactg gcgctgccag ccggtttgaa ttcatctcac
     1681 aaccctgcat agagcgaatc tcctgcctgc acgtcactct gaatcgccac gggtttaaca
     1741 gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat
     1801 cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc
     1861 agcgttgcaa cacgtctcga tatcaccacc cacag
//
LOCUS       DADLZM010000295         1879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLZM010000295 DADLZM010000000
VERSION     DADLZM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.300"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(53..409)
                     /locus_tag="JNY73_004207"
                     /pseudo
     CDS             complement(53..409)
                     /locus_tag="JNY73_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            489..1682
                     /locus_tag="JNY73_004208"
     CDS             489..1682
                     /locus_tag="JNY73_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY8049973.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1708..1879)
                     /locus_tag="JNY73_004209"
                     /pseudo
     CDS             complement(1708..1879)
                     /locus_tag="JNY73_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          512 a          430 c          464 g          473 t
ORIGIN      
        1 agttgcaact gcccggcgag cgccatcagt cgttgatgtt gcagttccat catcacgcca
       61 ctcctctgca gaatgagtcg tagatggaga gtggatgatg ctgggggggg ttgtcgaagc
      121 tcaccagatt ttcaccagga tgcacgtcat actctttttt ctccggaggc agtgccagca
      181 tggactgctg ctcttcgagc cagcgatcac agggacgggc ctggattgtt tcatgctttc
      241 gttggttagc gacatcgtgc agccagcgca gaccgtggcg gctggctgtt tcaacatcga
      301 cagtgctccc catcgggcgc aggcgagtca ttagtgggat gtaaaaactg ttacgggtgt
      361 actgcaccat ccgttccacc ttacctttag tctgtgccct gaagggctga ggaatcccca
      421 gaaaacgaga caaacatagc attatgtgat ctactgattt gtgccaacaa ttcaatgaga
      481 tcactgctat gcctgttcat aaagtatgtc agaatttctt ccgggatgcc ttagcaccat
      541 ttcatcagta ccgacaaaat gccctgatgg atgccacaat ggcgttaatc aacggagcat
      601 ctctgacgca gaccagtatc ggacgttttt tgccgggtaa tgcacaagtt aagaataaga
      661 tcaaacgtat tgatcgcctc atgggtaatg aggcgctcca tcgcgatatt ccaatgattt
      721 tcaggaatat cacttcaatg ctgacacggc agttatcttt atgtgtaata gccgtagact
      781 ggagtggcta tccctcccag gagcatcatg ttcttcgtgc cagccttctg tgtgacgggc
      841 gttccattcc gttattgagt aaggttgttc cttctgaaaa acaaaataat ccattgatac
      901 aacatgattt ccttgattcc cttgctcaat ccctgccccc cgatgccagg gttattattg
      961 tcacggatgc tggatttcag agcgcatggt ttcaccatat tacttctctg ggctgggatt
     1021 ttattggtcg gatcagaaat aacgttcagt attgcctcga taacgcgcct gaaagatggt
     1081 taaaagtgtc ggatagccct gaatgtaaaa caccggaata tatgggggct ggaaggctgg
     1141 ttaaagaaag gaaaaaaagc atccgggggc atttttacac ctataagaaa tcggcaaaag
     1201 ggcgaaaaaa gaaacgttcg aaaggtcaga gtggtttaaa caaaacagac aaagaacaga
     1261 gcaaatcagc aaaagaagcc tggttgatct tcagcagtac aaatgacttc agagcgcgag
     1321 aaatcatcaa gttatacagt cggcgaatgc agatagagca aaactttcgt gatgaaaaga
     1381 acgggcgctt cggatttggt cttcgggcca gcaaaagtcg ttcaacagga agaattctgg
     1441 ttctgagtct gctggcaacg ttgagcacta tcgtaatgtg gttactcggt tatcacgctg
     1501 aaaataaagg gttacacctg aaatatcagg ctaacagcat taaatcccgg cgggttatct
     1561 catatctgac gttagcgaag aatgttcttc gacactcgcc acttatttta agacgaacag
     1621 tactgagcac agttctaaac catctgtcca gaacctaccg aaatatggtg ctggtttatt
     1681 agcagtaatt ttgtggggat ccctcagcct gaaggggcga cacagtcggg gagagaagcc
     1741 catctccttg ccgaactgcc acagcgaagg atggaaccgg tgctgaccgg tctgatatgc
     1801 gtcacgttgc agaaccacag ttttcatatt gtcatacaac acttcgcgcg gcacaccacc
     1861 aaagaagcgg aacgcatta
//
LOCUS       DADLZM010000296         1869 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.301, whole genome shotgun sequence.
ACCESSION   DADLZM010000296 DADLZM010000000
VERSION     DADLZM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.301"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            27..1869
                     /gene="cyoB"
                     /locus_tag="JNY73_004210"
                     /pseudo
     CDS             27..1869
                     /gene="cyoB"
                     /locus_tag="JNY73_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          356 a          487 c          504 g          522 t
ORIGIN      
        1 attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt
       61 tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg
      121 ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg
      181 tcgaccataa acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg
      241 gttttgctga cgccattatg atgcgtagcc agcaggctct tgcctcggcg ggtgaagcgg
      301 gcttcctgcc acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct
      361 tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg
      421 gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt actgttgttg
      481 gcgtgattct ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg
      541 cctatccacc gctatcggga atagagtaca gtccgggagt cggtgttgat tactggatat
      601 ggcgtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca
      661 ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctaggcat
      721 cactgtgcac gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt
      781 tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca
      841 tgatgatgta catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc
      901 tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg
      961 gttatacctc gctggtatgg gcaaccgtct gcatcaccgt gctgtcgttc atcgtttggc
     1021 tgcaccactt ctttacgatg ggggcgggcg cgaacgtaaa cgccttcttt ggtatcacca
     1081 caatgattat cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc
     1141 agggccgcat cgtgttccat tctgcgatgc tgtggactat cggttttatc gtcaccttct
     1201 cggtgggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata
     1261 acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct
     1321 gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct
     1381 ggggtaaacg cgcgttctgg ttctggatca tcggcttctt cgttgccttt atgccgctgt
     1441 atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc
     1501 acaccatgct gatgattgca gccagcggtg cagtactgat tgcgctgggt attctctgcc
     1561 tcgttattca gatgtacgtt tctattcgcg accgcgacca gaaccgtgac ctgactggcg
     1621 acccgtgggg tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact
     1681 ttgccgtagt gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa gagaaaggcg
     1741 aagcgtataa aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgcag
     1801 gtatcgtcat tgcagctttc tccaccatct tcggtttcgc catgatctgg catatctggt
     1861 ggctggcga
//
LOCUS       DADLZM010000297         1828 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.302, whole genome shotgun sequence.
ACCESSION   DADLZM010000297 DADLZM010000000
VERSION     DADLZM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.302"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..168
                     /locus_tag="JNY73_004211"
     CDS             <1..168
                     /locus_tag="JNY73_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY8049974.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="JNY73_004212"
     CDS             153..791
                     /locus_tag="JNY73_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY8049975.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1410
                     /locus_tag="JNY73_004213"
                     /pseudo
     CDS             1019..1410
                     /locus_tag="JNY73_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1429..1800
                     /locus_tag="JNY73_004214"
                     /pseudo
     CDS             1429..1800
                     /locus_tag="JNY73_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          544 a          324 c          435 g          525 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagtgggggg ggagatgaac cctatgcaat gtcctggtta
     1441 atatctcagt gtgctcatca gtgcactgat aataaaaaga cggagacaga tgccatttac
     1501 gataaagttc ggagttctta tttgctttca tgtattctga aaaaaaacaa aaatgttggg
     1561 cttattcttc atgcaccatc atttgtttct gtgtctgaaa aaatcgccag aattgtgatg
     1621 gcaaattact cccgtaactg gagtaattcc gagttggcca gtgcggtttt aatgagtgaa
     1681 tcttctttaa aaagaagaat gtacaaagag gtcggttcta taagtacatt tgtacataaa
     1741 ataaagctta cagaagctat caggaaatta cgaaggacaa atactcccat ttcggtaatg
     1801 ctgccaactt actgatttag tgtatgat
//
LOCUS       DADLZM010000298         1816 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLZM010000298 DADLZM010000000
VERSION     DADLZM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.304"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(39..308)
                     /locus_tag="JNY73_004215"
                     /pseudo
     CDS             complement(39..308)
                     /locus_tag="JNY73_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(423..1061)
                     /locus_tag="JNY73_004216"
     CDS             complement(423..1061)
                     /locus_tag="JNY73_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049976.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1220..1690)
                     /locus_tag="JNY73_004217"
                     /pseudo
     CDS             complement(1220..1690)
                     /locus_tag="JNY73_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          512 a          448 c          335 g          521 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggagccagtc tacccagcaa gcgctgtaac
       61 tcttttattt gttttatagc ggcagccagt tctgaggcag gaacgacatc ctctccggca
      121 gcaacagcag tgagacttcc ttccagatat tattttttcc acttaagcaa caggctgggc
      181 tgaataccat gcaaacgcgc cacatgggac accgtcatgc caggctccat tgtctgctga
      241 ataatggcaa ttttttcctg aggagtttta cgtttacgaa gctccagccc cagcaaagtt
      301 ccagtcatct caaaattaga actagtgtta gacatatatc caagcctatc tcttatttta
      361 agatacatcc actgtctggc gtttcaaggg gccggctcaa atctatgtac aggctctcag
      421 catcagcaaa gcctcttact tttccaacta ctactgttag catagtgatc aaattcctga
      481 tcagatacat gaattacctg aaccgaacta ttttcatcta caaatgcatc tggcgatttt
      541 tctacaggat cataaagatt aaatcccttt cctgtttgaa catccaaaaa cataattttt
      601 tcaccatcgt taaatccagc tatccaatga gtggcctgtt caatagaaat cattactaca
      661 cttccttttg aagtattttg taaaatattt tctgcaatac gctcacgaaa tttactataa
      721 gtttcattag gatgtgacac tatgccttca ctatcaattt gctctaaatg cctatgaaaa
      781 aatccggtca tatactctga tatttcatta ggtacgggag cctctcttac tccataccct
      841 aacatagcca tacaagcatg aagcgcacac cctgaacagt taccatcccc accagacgga
      901 tttatcattt tctgtaaagt cgctgccaat tcagaatctt tatccatatc accaagactt
      961 ttagagttta aagattccag caatttttta acatttacat ttccctcctc tgtatgtgcc
     1021 tcatatccag cagttccctg taacgacacc ccattaatca tattgtatcc tgacagtata
     1081 tatactcact atattttctt cataacaaat atccctctga tatgacaaca aaaaacggag
     1141 ccggattcct gtttttgtga actgcatcta ttcgtgtcta aaaagtgatc cacttaccac
     1201 caacggccta atatatacag aacggagtaa acagggctaa cagttcccga caggtctcat
     1261 cattccgtgg cccgaaagtg taagccaaca caccgccagt tttagtgttg tacgcatacc
     1321 agagccagtg ttgttgcagc cttgctgcca acgaagcgcc actgctcatc aagttcacag
     1381 ataagggcaa catcagcgtg agcgactggc gcagatgtta tccgccgggg cgggagcttt
     1441 ttaatgtccg tatgacggta ttgatgccga ttttcagtgt cctggcggta tcacggcccc
     1501 ctgcgccgtt atgtgccatt tcaacgatct gctccttaac gccgggtttt ctggcttcat
     1561 aagagtaagt gagctggaaa acacgtttac atgagcgaca acggaaacgc tcatgccggg
     1621 aacaactacg gccatgtcga taaacttcat cggaatgaca gcgcggacaa tgaacagtaa
     1681 cggtagccat ggaaaacctt aaataccagt agattaccag aaacttctgt cgattgaaga
     1741 catcacccga agtttgaatt cattcgaata ctttttacga ggttcttttc gccagtcctg
     1801 taattcgtaa gcgccc
//
LOCUS       DADLZM010000299         1796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLZM010000299 DADLZM010000000
VERSION     DADLZM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.305"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(178..579)
                     /locus_tag="JNY73_004218"
                     /pseudo
     CDS             complement(178..579)
                     /locus_tag="JNY73_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1008..1796
                     /gene="virF"
                     /locus_tag="JNY73_004219"
     CDS             1008..1796
                     /gene="virF"
                     /locus_tag="JNY73_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY8049977.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          560 a          329 c          332 g          575 t
ORIGIN      
        1 cttccttatg ttatatttta gtaaagaatt tattttttac attatagtcg gctaaatgaa
       61 gtttactgta aaaaaacgat acttttttcg taagcgatga gatgtggagc cagaaaaatg
      121 gctgcgttac gtcattgaac tgtccttgac ccacgatatt cagacagtcg ttgtatctta
      181 agttgacatt acccgctgac gtcgatattc ccttggtgag agataaccca acgcactatg
      241 cggatggttt tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg ccgttcttgg
      301 tttcggcatg aacgcgatgc agtcttcctt catcgttttc acgaaccgtt ctgctattcc
      361 attactctga gggctactga ctgccgtcgt gcagggctcc agattcaact ctctggcgaa
      421 ctgccgtgtt tcatgagccg tataagccga accgttatcg gccactggat tggccgttcc
      481 ggtcctttat cgccatatcg ctcctgacat gacatcctgc accgttgctt tgtcataatc
      541 tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa tatggaacgt aacgctgtca
      601 tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc aatatcaaca ccaaccggat
      661 ttgtggagcc tccagtctga aggggcttta tgcgttccgt ataggatatt atgatgctgg
      721 agttttgcga agctccactc ctttccgaac atttacacag atattgccta agaaaagtga
      781 aaccataata tggttatagt ccctttcagt gcaaatactt agcttgttgc acagagaaat
      841 agaagctgca taagctcttt cttcaaaaaa tgtaaataaa gttaaatata ggaaaaatta
      901 cttaatctat cttaataacg gaaagatttt gtatacaatc actgttacag aaatttctta
      961 gttactctgt aaacactaaa tatagtttgg tatattctgt tgaatttatg atggatatgg
     1021 gacataaaaa caaaatagat ataaaggttc gcttgcataa ctatattatt ttatatgcaa
     1081 aaaggtgttc aatgacggtt agctcaggca atgaaacttt gactatcgat gaagggcaaa
     1141 ttgcttttat agagcgaaat atacaaataa acgtctccat aaaaaaatct gatagcatta
     1201 atccatttga gattataagc cttgacagaa atttattatt aagcattatt agaataatgg
     1261 aaccaattta ttcatttcaa cactcctatt ctgaggagaa aagggggtta aacaaaaaaa
     1321 tattcctcct ctctgaggag gaggtttcta tcgatttgtt caaatctata aaagagatgc
     1381 ctttcggcaa aagaaagatc tatagtttag cttgcctttt atcagctgtt tctgatgagg
     1441 aagctttata tacttcgata tcgatagctt cttctcttag tttttctgat cagataagga
     1501 agattgttga aaaaaacatc gagaagagat ggcgtctttc tgatatttca aataacttga
     1561 atttatcaga aatagctgtt agaaaacgat tggagagtga aaaattaaca tttcaacaaa
     1621 tccttcttga tattcgcatg catcatgcag caaagctttt attgaatagt caaagctata
     1681 ttaatgatgt atcaagactt atcggaatat caagcccatc ttattttata aggaaattta
     1741 atgaatatta tggtataact ccaaagaaat tttacttata tcataaaaaa ttttaa
//
LOCUS       DADLZM010000300         1782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLZM010000300 DADLZM010000000
VERSION     DADLZM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.306"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..452
                     /locus_tag="JNY73_004220"
     CDS             <1..452
                     /locus_tag="JNY73_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049978.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGRWYNAGSI"
     gene            455..988
                     /locus_tag="JNY73_004221"
     CDS             455..988
                     /locus_tag="JNY73_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8049979.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="JNY73_004222"
     CDS             complement(1017..1544)
                     /locus_tag="JNY73_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8049980.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1782)
                     /locus_tag="JNY73_004223"
                     /pseudo
     CDS             complement(1546..1782)
                     /locus_tag="JNY73_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435977.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          499 a          391 c          399 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggaggtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcaaag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cg
//
LOCUS       DADLZM010000301         1773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLZM010000301 DADLZM010000000
VERSION     DADLZM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.307"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..284
                     /locus_tag="JNY73_004224"
     CDS             <1..284
                     /locus_tag="JNY73_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049981.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="JNY73_004225"
                     /pseudo
     CDS             286..890
                     /locus_tag="JNY73_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1773
                     /locus_tag="JNY73_004226"
     CDS             877..>1773
                     /locus_tag="JNY73_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8049982.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLPDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          426 a          403 c          496 g          448 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggaaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 ccagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttc
//
LOCUS       DADLZM010000302         1746 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLZM010000302 DADLZM010000000
VERSION     DADLZM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.308"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            44..976
                     /locus_tag="JNY73_004227"
     CDS             44..976
                     /locus_tag="JNY73_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8049983.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1052..1126
                     /locus_tag="JNY73_004228"
     tRNA            1052..1126
                     /locus_tag="JNY73_004228"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Arg,seq:cct)
     gene            1288..>1746
                     /gene="intS"
                     /locus_tag="JNY73_004229"
     CDS             1288..>1746
                     /gene="intS"
                     /locus_tag="JNY73_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY8049984.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT          492 a          398 c          438 g          418 t
ORIGIN      
        1 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
       61 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
      121 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
      181 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
      241 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgtcg aactggaagg
      301 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
      361 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
      421 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
      481 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
      541 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
      601 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
      661 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
      721 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
      781 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
      841 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
      901 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
      961 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
     1021 agtccattag cgggtatact catgccgcat tgtcctctta gttaaatgga tataacgagc
     1081 ccctcctaag ggctaattgc aggttcgatt cctgcagggg acaccattta tcagttcgct
     1141 cccatccgta ccagtccgca aaatcccctg aatatcaagc cttccgtata ttcacagttc
     1201 gtcatggttc gcgtcagatc gttgacagcc gcactccatg acgggtaaaa agtggataaa
     1261 ataattttac ccaccggatt tttacccatg ctcaccgtta agcagattga agcagcaaag
     1321 ccgaaagaaa aaccataccg ccttctcgat ggtaatggcc tgtaccttta tgtccctgta
     1381 tccgggaaaa aggtatggca gcttcgctac aagattgacg gtaaggagaa aatcctgacc
     1441 gtcggaaaat atccgcttat gactttgcag gaggcaaggg ataaagcatg gactgcgagg
     1501 aaagacatct cggctggcat cgatccggta aaggcgaaaa aggcttcgtc taacaacaat
     1561 tcatttagtg cgatttacaa ggaatggtac gaacacaaga agcaagtctg gtcagtaggg
     1621 tatgcgactg aacttgcaaa aatgtttgat gacgacattt tacccatcat cggcggcctt
     1681 gagattcagg atattgagcc gatgcaactg ctggaagtaa tccgcagatt tgaagatcgc
     1741 ggtgca
//
LOCUS       DADLZM010000303         1735 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLZM010000303 DADLZM010000000
VERSION     DADLZM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.309"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            71..166
                     /locus_tag="JNY73_004230"
     CDS             71..166
                     /locus_tag="JNY73_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAY8049985.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="JNY73_004231"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="JNY73_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAY8049986.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          642 a          288 c          278 g          527 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc gtcgt
//
LOCUS       DADLZM010000304         1731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLZM010000304 DADLZM010000000
VERSION     DADLZM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.310"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(219..773)
                     /locus_tag="JNY73_004232"
     CDS             complement(219..773)
                     /locus_tag="JNY73_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY8049987.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(770..1060)
                     /locus_tag="JNY73_004233"
     CDS             complement(770..1060)
                     /locus_tag="JNY73_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY8049988.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1060..1659)
                     /locus_tag="JNY73_004234"
     CDS             complement(1060..1659)
                     /locus_tag="JNY73_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY8049989.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          415 a          477 c          394 g          445 t
ORIGIN      
        1 ggtggcgaac tgtgcagggt tggcgtaacc gggtacggaa accggcgagc ctttcggctc
       61 ccccacacag cccgccataa atcgcgaatg tgactgtgca aacgatatga aaaaagacgc
      121 gggcgcgtct catatcgctc cgtaaacatc cgggacgcca atcccgacgc cagattttgc
      181 tggcgcgtga ggaatatagc cccggataac agattgagtc aacaaacggt ttttagatcc
      241 ccggaagaga atgcatcacg catcggtaga tagagcatat attcagcaag atttagccat
      301 acatctaccc gactgcagca cgtccggaaa caccactcag ggtgtgcgtc attcagcaat
      361 tcagccattt tacgcttagt catcccccgc ccttcatacc gttgccggag gacactaatc
      421 aatccaggat gctctgccag cacctcactt atgactctat caatacataa cgcctctgca
      481 tcagtacaat gcgccagcca ggtcttttgc ttgccattga tcatctctcg caaaaacgct
      541 tccagctcag gtttctctat tcccgctttt ttcatcctgc gcagggcttc attgacggct
      601 gttttcgtca attttttgga tgccagcaac tggttaaaca tatttcccgt cttaccgccg
      661 ccaatatacg accagcgccc ccacatgcgc agttttccct gaatccagac actttccagc
      721 gtggtgagac gaaggtgttc cccgcttttg cctgtatttg ttgggtaaat cataaataac
      781 cttcctttct ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt
      841 cttctttggt gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg catgagctac
      901 aggcaatcgc tgcctgcata tcgtgtggtt ttgtcgctgt tccgcacgtc cccgccagcc
      961 tgtaatgcgc cagcacagaa gtttcgggat tgtgattgca gtagccagga attctgacgg
     1021 tgcacatctg cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagtaactg
     1081 cgcggccaca ttttcgactt cctccggaga ggaaaattta cggaacagga tccagttcca
     1141 cagcacattc agtacagatt tataaacctg ctgaaactcg gtttcgtcca tgttcgcaaa
     1201 tgcgatagat tttgccctgc gcccacgact accatcagga taaatatgct cggtgtaaaa
     1261 tccggcctga atggttaccc actcgcggaa agcgtcaaac gactttagca atgccgtatc
     1321 ccgggttctg catgtcgcaa ctgtattaag gtattgctct gcggcatcac tcagggctgg
     1381 agtgtgttcc cgacctactg attcgcacag gtaatcaacg aagcctgata ccagttttcg
     1441 ttcgcgaggc gtgatcgccc caccgaccgg agtccagtaa tcaaatccca gttgcaggag
     1501 tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca
     1561 ctcacctatt ttgatttgat gcaaaaaatc gcaactctcc ggcgtcgccg ggagaagtaa
     1621 tccggaagag gtttgtttga ccagttgtat atgcgccatc gtagttctcc gctggcgcag
     1681 tagaatgggt gttcagcccg ttatgtagta taccagaatt aatgccaata c
//
LOCUS       DADLZM010000305         1726 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.311, whole genome shotgun sequence.
ACCESSION   DADLZM010000305 DADLZM010000000
VERSION     DADLZM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.311"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..546)
                     /locus_tag="JNY73_004235"
     CDS             complement(<1..546)
                     /locus_tag="JNY73_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049990.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..>1726)
                     /locus_tag="JNY73_004236"
     CDS             complement(1208..>1726)
                     /locus_tag="JNY73_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049991.1"
                     /translation="SKMVDVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTESSF
                     GSEVTDYGIIAHESHLKALSRLAAKQGFGGEDAIVDMLGTDIAYYNGADRGVFMMAKR
                     FTALSFGCFRHDGENITVVLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVL
                     PVVKRLSFTKTS"
BASE COUNT          493 a          385 c          388 g          460 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagatta atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgcaccc acgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga tgtctgtacc cagcatatcg acaatagcgt
     1501 cttcgccacc gaagccctgc ttagcagcca gacgggaaag agccttgagg tgtgactcat
     1561 gggcgatgat accgtaatca gtaacctcag aaccgaaaga agactcggtg ttctcataca
     1621 gagcgtccat gatagtgtct gccttagcgg tcaggctacc agtcaggacc ggaagctcta
     1681 cggtcttcag cagcttagag gactccagaa cgtcaaccat cttaga
//
LOCUS       DADLZM010000306         1684 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.313, whole genome shotgun sequence.
ACCESSION   DADLZM010000306 DADLZM010000000
VERSION     DADLZM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.313"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..986)
                     /locus_tag="JNY73_004237"
     CDS             complement(<1..986)
                     /locus_tag="JNY73_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8049992.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYK"
BASE COUNT          493 a          256 c          315 g          620 t
ORIGIN      
        1 tgtttataac atattttttt gaactcttgc ttatatttta atatgtcatc aaatgaaaga
       61 ttcactacct ttgcctctaa gaaatcaatt attctcgaag caatttccgc tttcgcgcgt
      121 aaagagttgt cattaatagt taacaatgta gaaaacaagc cattgatttg tttgtcaaga
      181 tgttgttcat atttatgtag tttggggatg ggattttcct tgtgtgaaaa aatagtgtta
      241 aataaatttg cgtcagtgaa atttatatta agtagatctt caaaataaaa ttttgataag
      301 gttaggacta gcttggacat atcttgccct gtcatgttga aatcaataaa gtcgcatcca
      361 gcaagggctt ccatcctatt ttcattttta ctgtgctcaa tatacagctt cgccattttt
      421 aagtacaagc tttgaatgtc tcttatatcg gttggtaagt cagctcgttg cccagcaaaa
      481 tttttattat cggaagaaac ggtttggaca ctgaatcttt cgggtattcc ttcttctacg
      541 agaattttag ttatgattgg attgttgggt tctggattaa cgacaacaat gatttgacat
      601 acagaatcct tatccttttc gtatctaatg ttatgaatct gattaagtat tgattgtgtc
      661 tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact tacaagttga
      721 accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt
      781 tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc tccagagaag
      841 gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc
      901 gttttaacat tatcggtatt tgttgtgcag ttataaatat cgccgtgata tgtatttatt
      961 atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt ttatgctaat
     1021 gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa taaatataat
     1081 gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac ccggtaatat
     1141 ttgtgtataa aaaaagggca gtgatagtat cgtaaaggaa aacgttaaca actatcaaaa
     1201 agcgctcatt ttaaaaagtt attatgttct ccgatagtgt gtgaagtaat atttttgtga
     1261 tgttttagat gtgtaataat gaaccaggtt aaagtgtatt taaaaatgtg ttgtattagc
     1321 gactgaaatc tgccttttaa aaaggaggag ggtactgaga caaagagtca ttgcttctcc
     1381 gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact tgttgaccga
     1441 gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga ttatcctgtc
     1501 tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt tactgctggc
     1561 atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac ctaatataac
     1621 cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata tcacttccgt
     1681 cagg
//
LOCUS       DADLZM010000307         1679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLZM010000307 DADLZM010000000
VERSION     DADLZM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.314"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(72..539)
                     /locus_tag="JNY73_004238"
     CDS             complement(72..539)
                     /locus_tag="JNY73_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY8049993.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFFALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="JNY73_004239"
     CDS             complement(605..844)
                     /locus_tag="JNY73_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY8049994.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="JNY73_004240"
     CDS             complement(979..1584)
                     /locus_tag="JNY73_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8049995.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          543 a          369 c          294 g          473 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc aaaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DADLZM010000308         1662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.315, whole genome shotgun sequence.
ACCESSION   DADLZM010000308 DADLZM010000000
VERSION     DADLZM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.315"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..933)
                     /locus_tag="JNY73_004241"
                     /pseudo
     CDS             complement(1..933)
                     /locus_tag="JNY73_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            complement(961..>1662)
                     /locus_tag="JNY73_004242"
     CDS             complement(961..>1662)
                     /locus_tag="JNY73_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8049996.1"
                     /translation="ESWGINTTYVEKVPCTSSGVAPIFVNANSSNSILIIKGANKFLS
                     PEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYA
                     CKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQ
                     EVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGMQSS
                     YPSIEQFNEFLTLNE"
BASE COUNT          519 a          366 c          328 g          449 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagaccatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt ggaaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaatacctg acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aagtgtacaa ccaccaatat acagcgtcag
      661 gccaattaaa attgccacct tataactggt ctttttaatc acaagggatg ctggtattgc
      721 aattaaaaaa taacctccat aaaatgcgct ctgcaccaat gctgaagcaa agttacttag
      781 cgaaaataca cttttgaatt gagtgattaa tatgtcattt aatgcagctg cgcatcccca
      841 tagcgggaat aaacacgata acaaaataaa ctggaacaag ggagtcttat tcagataccc
      901 atcaggcatc tgaatgatgt ttttatcgtt catagtgcta cctttaactg tgcaggatga
      961 ttattcgttt aaggttaaaa attcattaaa ttgttcaata ctcggataag atgattgcat
     1021 acctttccct gtgacgctga aagcggcaaa gagagcggct tttttcaaag cggcttcaac
     1081 atcaccgctt tgaacataat aatgggaaaa gcaaccaata aatgcgtcac cagcgccact
     1141 agtatcaaca gcatttactt tgaatgcagg aacatggact tcctgatcgc gggtcatcca
     1201 taatgcgcct ttttcgctca tggtaacaat aatattgttc agccctttat caactaacga
     1261 acgtgcggcc aaacgaatat gatcataagt atcaaccgac ataccggtta atatttccag
     1321 ttctgtttca ttcgggataa agaaatcacc tttgcaggca taagacatat ctaactcacg
     1381 caatgccgga gccggattta ataacacttc aataccattt ttcttaccaa actcaatcgc
     1441 gtggtaaact gtttccagtt gaacttccag ttgtaaaacg atcaatttgc attttttcag
     1501 atcttctgca gctcgatcga tatcttccgg ggaaagaaat ttattcgctc ccttaattat
     1561 taatatacta ttgctcgagt tggcattaac aaagatcggt gcaacaccac tgctggtaca
     1621 ggggaccttc tcaacataag tggtattaat tccccatgat tc
//
LOCUS       DADLZM010000309         1654 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLZM010000309 DADLZM010000000
VERSION     DADLZM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.316"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(155..1390)
                     /locus_tag="JNY73_004243"
     CDS             complement(155..1390)
                     /locus_tag="JNY73_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY8049997.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1537..1613)
                     /locus_tag="JNY73_004244"
     tRNA            complement(1537..1613)
                     /locus_tag="JNY73_004244"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Pro,seq:tgg)
BASE COUNT          422 a          452 c          391 g          389 t
ORIGIN      
        1 ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg
       61 caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca
      121 atcaaccgca ggcggcttcc aaatggccgc ttttttactt attcaccacc atcaaatgtg
      181 cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt gctttaagat
      241 atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt
      301 tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccaacat gcttttaaca
      361 cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca aacacctgct
      421 gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc agccgatatt
      481 gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc
      541 gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg
      601 cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag tggtcaaaaa
      661 tcgccaccag aaattcacca aattcatcgg ggcgcacaga ccattccgtc agttccccct
      721 gaatatcacc aggcgcatgt agtttcagtc catcgcggac agctgtttca tcagccaggc
      781 gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa aaatcataaa
      841 cctgtaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta tagtcgacat
      901 gatgtttttg caggagcgtc agggcacgca tcaccagttt atgcgtgggt ctgccgcctt
      961 tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa
     1021 aattattttc tgccagaaat gcacaccatt cgtcatcgag cagcacgccg ttagtctgga
     1081 aactgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg gcacggcggt
     1141 aaaactccag cccgagtagc gttggttcgc caccctgcca agtaaaagcc acttcgtttt
     1201 gcagttcgct ggcggcgata tagtgacgga tatacgcttc cagcgtgtca tcgtccatat
     1261 gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt
     1321 tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct
     1381 gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat
     1441 accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca
     1501 gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac
     1561 ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact
     1621 gctttttgaa tttttagttc aattctttaa agtc
//
LOCUS       DADLZM010000310         1646 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLZM010000310 DADLZM010000000
VERSION     DADLZM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.317"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(821..1060)
                     /locus_tag="JNY73_004245"
     CDS             complement(821..1060)
                     /locus_tag="JNY73_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY8049998.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1255..1621)
                     /locus_tag="JNY73_004246"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="JNY73_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          366 a          456 c          396 g          428 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctacttgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacttgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccgaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgc atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggt gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggc
//
LOCUS       DADLZM010000311         1636 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLZM010000311 DADLZM010000000
VERSION     DADLZM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.318"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(161..1021)
                     /locus_tag="JNY73_004247"
     CDS             complement(161..1021)
                     /locus_tag="JNY73_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY8049999.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1204..1605)
                     /locus_tag="JNY73_004248"
     CDS             complement(1204..1605)
                     /locus_tag="JNY73_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8050000.1"
                     /translation="MKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDG
                     ISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTL
                     HQKGTGATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT          419 a          420 c          356 g          441 t
ORIGIN      
        1 atctctgctc tcaggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
       61 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
      361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
      421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
      481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
      541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
      601 cgatcgttgt cagaagtaag ttggcagcag tgttatcact catggttatg gcagcactgc
      661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
      721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
      781 gggataatac cgcaccacat agcagaactt taaaagtgct catcattgga aaacgttctt
      841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
      901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
      961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acgaaaatgt tgaatactca
     1021 tactcttcct ttttcaatat tattgaagca tttaccaggg ttattgtctc atgagcggat
     1081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1141 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1201 gtatcacgag gccctttcgt cttcaagaat tttataaacc gtggagcggg caatactgag
     1261 ctgatgagca atttccgttg caccagtgcc cttctgatga agcgtcagca cgacgttcct
     1321 gtccacggta cgcctgcggc caaatttgat tcctttcagc tttgcttcct gtcggccctc
     1381 attcgtgcgt tctaggatcc tccggcgttc agcctgtgcc acagccgaca ggatggtgac
     1441 caccatttgc cccatatcac cgtcggtact gatcccgtca tcaatgaacc ggactgccac
     1501 gccctgagcg tcaaattcct ttatcagttg gatcatatcg gcagtgtcgc ggccaagacg
     1561 gtcgagcttc ttaaccagaa tgacatcacc ttcctccacc ttcatcctca gcaaatccag
     1621 cccttcccgg tctgtt
//
LOCUS       DADLZM010000312         1630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLZM010000312 DADLZM010000000
VERSION     DADLZM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.319"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..443)
                     /locus_tag="JNY73_004249"
     CDS             complement(<1..443)
                     /locus_tag="JNY73_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY8050001.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
     gene            complement(488..1451)
                     /locus_tag="JNY73_004250"
                     /pseudo
     CDS             complement(488..1451)
                     /locus_tag="JNY73_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          465 a          352 c          330 g          483 t
ORIGIN      
        1 gcataaaaca tcattaccac tccacttaat ggcagaatga tatatacgta tcccatcggt
       61 atttgcatag cggcagatac ctgtgatgtt gccagtgttt tatcaattaa tttcaggcca
      121 ccagtcacaa tgaccgaacc ggcaaaacct aaaatgagaa cgttaatgac tatactaaga
      181 agtaattgtt tgcgcttatt cagggcgaga gcaaataaat caatagagag atgtcgctgc
      241 gcgccgacgg tatatgcagc acctaataat cctacccaga tcatcaaaaa gcgtgccagc
      301 tcatcggtta atgtacttgg ctggttaagt acgtagcggc taaatacttg ccagactacg
      361 catacaacca gagcaagcat tacggcgaca gaaaacgcgg caatgacgcg gtctaccgca
      421 agtttcaggc ggttaaatat cattgtaagc atcctggaaa atcagggcca gaggctggcc
      481 cggaatatta ttgagcagcg ttgtcgaatt tctctaacaa agcagcttgt tttggatctt
      541 ttttgaagtc gtcaaacaga ggttgcacgg ctttacggaa tggcgcttta tccactttta
      601 caatttcacc ccccatagct ttcgcctgag cacgtgtgtc agcgtcaatt ttttcccaca
      661 atttttgttg ataagcttcg gatttcttcg ccgctgtttc gaggatttgt tgctgttctg
      721 ggggtaattt attccatgtt ttggtggaga taaccaaaaa gtcaggaatt gaggcgtgtt
      781 catcttcaga aaaaactttg gcaatttcaa tatgtcgagt ctgcatccag gaaggcacgt
      841 tattttctgc gccatctact acaccttgct gcatcgcagt ataaacttcg ccaaatgaaa
      901 ttggagtggg tgaaccaccc atcagttcaa tcattttgat ggtggtcgga cttggctgaa
      961 cacgaatttt cagtccttta agatcttctg gctttgtaat cggttttttc gcatagaaac
     1021 tgcgagtacc cgctacataa gctgataaag caaaaaagcc tttgcctttt gttgagtcca
     1081 taatttcttt accaacttca ccaaaaacaa cttaataaga acggcaaatt atagatagca
     1141 taaatattat caaaagactc cagatcactg gcggaacctt tggtcatatc cagtgcgcca
     1201 ttttgcaaca attccatagt ttctcgcgca ttacccatct ggctgctggg ataaatacgg
     1261 atcaccatat taccttttga cagctgtttt acctctttcg ccagctcttc aaaactctga
     1321 tgtacaacat gacttctttc caggttgtgt gctaacttta acgtcacttt ttctgcggcg
     1381 tttacgccag tgctaaccag agccagcaag gatgcgcaga ctaatgagcg ggtaaatgac
     1441 ctcattttca taaaaaaact ccctgttgaa ggatggaaat tggttgatat ctaattgtgc
     1501 ttatcagtgc tttgtgcgac aaattggtta acctgtctga caacttgtct atgtttatca
     1561 caacttgaaa taaagtcaga aattaaatat taaatttgtg cgccaggttg tacatattta
     1621 taatcaatga
//
LOCUS       DADLZM010000313         1604 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLZM010000313 DADLZM010000000
VERSION     DADLZM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.320"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>1604)
                     /locus_tag="JNY73_004251"
     CDS             complement(<1..>1604)
                     /locus_tag="JNY73_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8050002.1"
                     /translation="IYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPYA
                     SLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGSA
                     ALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYRY
                     STEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWHT
                     DDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSG
                     WWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTY
                     DGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLEI
                     DNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKA
                     EFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQV
                     TLSWGENASTKCIFNYSLSTPESESG"
BASE COUNT          412 a          415 c          346 g          431 t
ORIGIN      
        1 atccgctctc actttctggt gtcgaaagtg aataattgaa gatgcatttt gttgaggcgt
       61 tttctcccca ggataacgta acctgtcctt tttctggtag accagaaaga taaagttcgc
      121 cacggtcacc gacaattcct gaatctgcca aacttgcatc ttgtatcgca gcaatggctc
      181 caaaaggtac cggtttacca tttgcattta acactctgaa gagaacgcga taacccacat
      241 gagtcacaaa ctccgcttta accactgcac cacgcgtggg aaccatcgta acgacggtat
      301 tatcaagttc tatattcgac ttcgagaaat agttatcgct gattgctacc cgattttcat
      361 cataagggtt tagctgtgtc ttgaccgtat agccgcgcca gtccgtcgca gcaccgcgca
      421 tattatctat ttctaacccg gcggccccag gtgctttaac aagagcaata gtttcaccta
      481 actcctgact taacgttacc ccttcgctgt gtgccagaat tccccctctg ataccataat
      541 tcagcctctg ggaattttgg ctgtagttat agctgccgtt caccgtgcca taagagccat
      601 cataggtcag ggacatatta ccgccaacct catcatcatg actgttatag gactgcatta
      661 aattccatga aaggttattt ctttccagcg ccgtaccgct gagtccgagt tgatgtgagc
      721 taccggaatt atcgttttgc gaaacagaat aggtggcgta actattaccc caccagcccg
      781 ataaaggaac agataaagag agcatgtaga tctgctcact ggtgttatca ctggcatggt
      841 tgttccccgt cccctgtctg gcagtattgg aataactcca ggagacgttc cacgagagac
      901 gcttccagct actgctgtag ccaaattgca ttaatcgttc gacatcatcg gtatgccagt
      961 attgttgccg gcttaatgtc aggtataaat tgccataatc tctccccaac gactgcgtta
     1021 gcgttccctc cacgcgacta cgtcggttac ctgtttccca aaagtcttca gggctgttgc
     1081 gccgcgatgc ccactcattg agggtatagt agccctcggt ggagtaacga tagccaacca
     1141 ggcgcaagct agtgtctgtg tcgtcaaata gttttgaata gagaaagcga tatgactgac
     1201 ccgtttctgt atcatcatgg ctaaaattcg cacgagcatg tgtcacatcg aaagaaattg
     1261 cgcccaaagc gccaagatct ttaccgacac ccagagctgc agaaccataa ttttcagcag
     1321 cctgcatacc tccatataaa gtgatcccgt gggaaaaacc atgaagtatt tgcgcctgaa
     1381 ggacgtccgg tgtaaatccg tcttcatcgc gcaattcacc cacgctgaca tcaacatctg
     1441 tcagaccttc acgtttaaga atcgccaatg aagcataagg ttgggtgaat cgttgttcgc
     1501 taccgtcttc ttctttgatg gttacgtcca aatcgccacc tgtacttgcg gtgttgagat
     1561 cggtgatctc aaaagcacct tgtggaacgg ctgactgata aatc
//
LOCUS       DADLZM010000314         1555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLZM010000314 DADLZM010000000
VERSION     DADLZM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.321"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(435..626)
                     /locus_tag="JNY73_004252"
     CDS             complement(435..626)
                     /locus_tag="JNY73_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY8050003.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            691..1014
                     /locus_tag="JNY73_004253"
     CDS             691..1014
                     /locus_tag="JNY73_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY8050004.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1004..>1555
                     /locus_tag="JNY73_004254"
     CDS             1004..>1555
                     /locus_tag="JNY73_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY8050005.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELTPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQALAESVTRGIDALYHV"
BASE COUNT          331 a          410 c          476 g          338 t
ORIGIN      
        1 gggcggagcc tatggaaaaa cgccagcggc gcagcctttc tttgcccttt gcctttttgc
       61 tcacatgttc tttccggcct tatcccctga ttctgtggat caccgtatta ccgtctttga
      121 gtgcgctgat accgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga
      181 agcggaagag cgccggaagc tgcattttct ccttacgcat ctgtgcggca tttcacatcg
      241 gctatggtgc acttttcata caatccgcac tgatgccgca taagtaagcc tgtgtacact
      301 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgctaaa acctgctgac
      361 gcgccctgac gggcttgtct gctcccggca tccgctcaca gacaagctgt gaccgtctcc
      421 gggagctgca tatgtcagag gttttcaccg tcatccccga aacgtgcgag gcagctgcgg
      481 taaagctcat cggcgtggtc gtgaagcgat tcacagatat cagcctgttc atccgcgtcc
      541 agttcgttga gcttctccag cagcgttaat gtctggcttc tgataaagcg ggccatgtta
      601 agggcggttt tttcctgttt agtcactgat gcctccgtgt aagggggatt tctgttttat
      661 ggggtaatga taccgatgaa tcgcgagagg atgctcacca tacgggttac tgatgatgaa
      721 catgcccggt tactggaacg ctgtgagggt aaacagctgg cggtatggat gcggcgggtc
      781 tgcctgggtg agccggttgc ccgttccggg aaactgccga cactggcacc gccgttactg
      841 cgtcagctgg ctgccatcgg gaataacctg aatcagacag cgaggaaggt gaacagcggg
      901 cagtggtctt ccggtaaccg ggttcaggtg gtggccgcac tgatggccat cggggacgag
      961 ctgcgccggc tgcgtctggc cgtcagggaa caggggacgc gggatgatag ttaagtttca
     1021 tgcccgggga aaaggtggtg gcagtggtcc ggttgattac ctgctgggaa gggagcgtaa
     1081 ccgggaaggg gcaacggtgc ttcagggtaa tccggaagaa gtccgggaac tcatcgatgc
     1141 cacgccattt gcgaagaaat acacgtccgg tgttctgtcg ttcgcggaga aggagctgac
     1201 gccgggagga cgtgaaaaag tgatggcgag ctttgagcgt gtactgatgc ccggtctcga
     1261 aaaaaatcag tacagcatcc tgtgggtgga gcaccaggac aagggacggc ttgagctgaa
     1321 ttttgtcatt ccgaacatgg agctacagac cggaaaacgc ctccagccgt actacgaccg
     1381 cgcagacagg cctagaattg atgcctggca gacgctggtt aatcaccatt acgggctgca
     1441 tgacccgaac gccccggaga accgcaggac gctgacactc cctgataatc tgccggaaac
     1501 gaaacaggcg cttgcagaga gcgtcacacg cgggatagac gctctttatc atgtc
//
LOCUS       DADLZM010000315         1540 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.322, whole genome shotgun sequence.
ACCESSION   DADLZM010000315 DADLZM010000000
VERSION     DADLZM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.322"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..149)
                     /locus_tag="JNY73_004255"
     CDS             complement(<1..149)
                     /locus_tag="JNY73_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mxiK"
                     /protein_id="HAY8050006.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIIN"
     gene            complement(146..871)
                     /locus_tag="JNY73_004256"
     CDS             complement(146..871)
                     /locus_tag="JNY73_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY8050007.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(877..1170)
                     /gene="mxiI"
                     /locus_tag="JNY73_004257"
     CDS             complement(877..1170)
                     /gene="mxiI"
                     /locus_tag="JNY73_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY8050008.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(1183..1434)
                     /gene="mxiH"
                     /locus_tag="JNY73_004258"
     CDS             complement(1183..1434)
                     /gene="mxiH"
                     /locus_tag="JNY73_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY8050009.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(1442..1540)
                     /locus_tag="JNY73_004259"
                     /pseudo
     CDS             complement(1442..1540)
                     /locus_tag="JNY73_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          486 a          297 c          211 g          546 t
ORIGIN      
        1 ttattaatta tgagattatt aatctcactt ctgattacgc cattttctaa cagttcagaa
       61 ggcaaattca accgatttct atttatatag aacgctggat caaaaattat tgaaagatat
      121 tttttataaa ttccatccat tcttatcata ttttgtttct cttgaaccac cctgttttga
      181 atgcccatac caaaagaatg acaactagta cagccatccc gagaaataaa tatattactt
      241 catttgttaa aaattccgat ttaacttcct taacaggttg tacatttgta taaacatatt
      301 cttctttcgg agttaatatg acagatatat tttcatactt aacatcagaa aaggtgtttt
      361 tcaaaaatcg cttaatatta ctaactaata gttcagactc tttcggtgag tcatatatag
      421 cgattactga tatatgcatc ggttttgaag atatattttt ttcttcaaga tcatagctaa
      481 catgtatttt tgccgaaata acaccaccaa tagaaactaa agactgttcc agccgttgct
      541 caatagcact atataaacgg gccttttcag ctcttggaga agacactaat gaatctgtag
      601 gaaacatttg tgagatatca actctctccg gatttggcaa atcgtacatg cgcatcaaat
      661 caactgccga tgcaaatgtc cccttttcga cttgtaccga gatcccctgt ttacctccat
      721 caacttttct agcagtaata ttatggcgtt ctagcacaga tattatttca tttgcctgtc
      781 tttgagataa attagaaatt aactcttcac gttgctcaca tccaatcaac atcagcaaca
      841 agaataaaat aaaaccttta tacctaatca tataaattaa gactttaata aagtttcaac
      901 agccgaaact gtttttcttg ctaacgtgcc agctaatgat actcctattg aataatttga
      961 gagcgtcgtc tgtaacttgg ccaaagattc tgggtttaat gattctggat tgcttaccat
     1021 ctccattatt tgtgatactt gaatatctgt atccatctta atatcactat atttagccga
     1081 cacgacgtct tccagacttg atatctcttg agattgaaaa tcactggctt tgataatatc
     1141 aacctgattg actggataaa tgtaattcat gaatgctccc tattatctga agttttgaat
     1201 aattgcagca tcaacatcct taatcacttt cactgtattg gattgcgcgt tcctatataa
     1261 tgtatattca gataatttac tttggtattc agccagcaac tgtggattcg aaggatttct
     1321 agctaattta tctagtgcca atgttagttc accttgtaat gtttgagttc catcatcaaa
     1381 agtttcagat aatgaactca atgtccaatc atcattcggt actgtaacac tcattttatc
     1441 ctcacttatt tttatctaaa aaaaaaccag tgtttatcat tcaacattac ataactgtct
     1501 ttgctgttaa gatatgattt acctttaaaa tcatcatcta
//
LOCUS       DADLZM010000316         1473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLZM010000316 DADLZM010000000
VERSION     DADLZM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.323"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1184)
                     /locus_tag="JNY73_004260"
     CDS             complement(<1..1184)
                     /locus_tag="JNY73_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY8050010.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RAL"
     gene            complement(1184..>1473)
                     /locus_tag="JNY73_004261"
     CDS             complement(1184..>1473)
                     /locus_tag="JNY73_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HAY8050011.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          285 a          489 c          343 g          356 t
ORIGIN      
        1 agtgcccgga cggacagctc atcgagcaca tgaattcctg atgtgaacag ccccttctca
       61 cggtcgaggg ggataagctg ctcacggctg atggcctcat cgataccggc gcgtgcccgt
      121 tcaatcacac cattttccgg cggcagtatg ccgaccgtcc tggccagcac gtccgtatac
      181 gtgaactgca cttttcgttc acttaatccg gcaatcgcct gtgtcaccgc ctgctgcaca
      241 tccggcccgt cctgtgaagc aggcccgggc gcctgcgtgc ggatctccgc acgctgatcc
      301 gccgcgtcac gatatgcccg gatgtcgaac ccggtttcct tcagcgtctg catccattca
      361 gccattctga tttccggatc gacgtgctgt ttggattttc gcgtatccag tgccgccaca
      421 tcccgggatt tcagcgaggc gtcttcaccg acggcctccc tgatagcctg tgaacgactg
      481 gaaaaggcct ccaccggtac gcccggcatc tcccacatac cgtgcttacc gaccacttcg
      541 gtttcataac ccagcgcctc aacgcgctga cgtaactcac tctgatatat tttcccgaac
      601 gcaatccggt tcgcgagcac gttctcggaa aatccggttt tccccacttt gtcactgctc
      661 agtgtcttcc actcgccgtt atgctgcgtg acattagcca ccaccacatg cgtgtgtaac
      721 tgtgggtcct gatcgcgact ggtgtcatgg ttaaacagtg ccatcaccag attaccggtc
      781 agcaccgttt ctgactgtcc gtccgtcatc acccgtgtgg aagccagcgc ctccacctga
      841 cggacagcaa aatccacggc ctggttatgt gcatcaataa gacgtttatc ccccccgagc
      901 atggccatca tggagacact tttgggggcg gagaaggtca ggtcgtagcc gggacgatgc
      961 ttattactgc catcctgcat gcggcttaaa tccgccccgt ccggcagcct gccctccaga
     1021 agacgggtaa aaacatcctt atcgacgctg ccctgcagcc caagctgttc agccccctga
     1081 ccggcccagc gttctcccat gctgcccagc acatagtaat tatccttgtc ggtgtaatag
     1141 tttccggcac tgcctgccga tttaacaacg ctgaaactca acatcagaaa tcatctcccg
     1201 gctcaacatc ctccccgcgc tcccggtgca cattaatgtt cacctcttca cgacgctgca
     1261 tatgctgctg gatgtccgga tgattttcct gttgccatgc ctcataagcg gccatatcca
     1321 ccacttcacc ggattcactg atcccgggtg gcagttgctg ttccatctct tcttccggtt
     1381 tcattttcag ctcctgctcg atcccccctg ccggaacact cacacctgca tctgacttct
     1441 tatcattgat gacagaagac actggctgtt gtg
//
LOCUS       DADLZM010000317         1446 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLZM010000317 DADLZM010000000
VERSION     DADLZM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.324"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..1046)
                     /locus_tag="JNY73_004262"
     CDS             complement(<1..1046)
                     /locus_tag="JNY73_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY8050012.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          433 a          231 c          306 g          476 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctatt
//
LOCUS       DADLZM010000318         1388 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLZM010000318 DADLZM010000000
VERSION     DADLZM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.325"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            226..495
                     /locus_tag="JNY73_004263"
     CDS             226..495
                     /locus_tag="JNY73_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050013.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            492..604
                     /locus_tag="JNY73_004264"
                     /pseudo
     CDS             492..604
                     /locus_tag="JNY73_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            747..1337
                     /gene="ospG"
                     /locus_tag="JNY73_004265"
     CDS             747..1337
                     /gene="ospG"
                     /locus_tag="JNY73_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY8050014.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          403 a          272 c          335 g          378 t
ORIGIN      
        1 caactgcacc attcataatc acatcgcata aaactgtcag cgccagtgat aggacaggtg
       61 aaacctgtca atacgtaccg gcttacgccg ggcttcggcg gttttactcc tctgccatat
      121 gcaacaacgg agtgccgcct tacatgccgc tggcgcggca tatcctggtg acgatatctg
      181 aacagttata tactgtgtat atacgtggta atgacgaggt ggcaaatggc acaggttaat
      241 atgagtgtaa gaatcgacgc agaactgaag gatgctttta tggccgctgc aaaaagcatg
      301 gaccgtaatg gctctcagtt aatccgggat tttatgcgcc agaccgttga acggcagcac
      361 aattcttggt tccgtgacca ggttgcggca ggccgtcagc aactcgaatg cggtgatgtg
      421 cttccccatg acatggtcga atcctctgct gctgcatggc gggatgaaat gagcaggaag
      481 attgctgata aatgatggag atgttctgga ccatgctggc catccaggac aggaagcgca
      541 ttcgtagtac attgcggagc agaacctgat ggccgccatt gaaccggacg aacggattgg
      601 ttattcagcc agcagcctgg cagggcagcc gtataaaggc cgcaacggtc gtgtggaagg
      661 aacatctgga ccacacaagg tagcgtgtaa cgttattttg tgtgaaaatc tcttgtgatt
      721 tttaaatatc gtaacaaagg tatgacatga aaataacatc taccattatt caaacacctt
      781 ttccatttga gaataataat tctcatgctg gcatagtaac ggagcccatt ctcggtaagt
      841 taataggtca ggggtcgaca gcagaaatct ttgaagatgt gaatgattca tctgctttgt
      901 ataaaaagta tgatcttatt ggcaaccagt acaatgagat tctggaaatg gcttggcaag
      961 aatctgagct ttttaatgct ttttatggcg atgaagcatc cgttgttata cagtatggcg
     1021 gagatgtgta cctccgaatg ctgcgcgtgc ctgggactcc ccttagtgac attgatacag
     1081 ctgatatccc tgataatata gagagccttt atctacagtt gatatgtaaa ttgaatgagt
     1141 tgagtataat ccattacgat cttaatacag gtaatatgct gtatgataaa gaaagtgaaa
     1201 gtttattccc aatagatttt cgcaatattt atgctgaata ttacgctgca accaaaaaag
     1261 ataaagagat tatcgaccga cgattacaaa tgcgtacaaa tgatttttat tcgttattaa
     1321 acaggaaata tttatagccg tatttgttga tgctataagc cagaggctct tctatgagaa
     1381 tatgggga
//
LOCUS       DADLZM010000319         1387 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLZM010000319 DADLZM010000000
VERSION     DADLZM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.326"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..573)
                     /locus_tag="JNY73_004266"
                     /pseudo
     CDS             complement(1..573)
                     /locus_tag="JNY73_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(621..1115)
                     /locus_tag="JNY73_004267"
     CDS             complement(621..1115)
                     /locus_tag="JNY73_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8050015.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          423 a          280 c          261 g          423 t
ORIGIN      
        1 catcacactg atatgaattg ggttgggatt ctctgctatc agatttcgcc cttcccggcg
       61 ccatttcact ttcggcgcag catcttcggg aataaggcgg atatttgctg gtcgccagaa
      121 cagcttgata cgcgttttta ctgctatttc cagcgagttc gtcgctttgt tgctcgctga
      181 tggcggaata tttttaatat tcagccagaa gactgactcg cgatcatcag gcaatgacac
      241 acctttggtg tgaataatgc gtaatatttt ttcttgcgaa ggttctaatc gtgatactgg
      301 cggaataacg gtaaacggcg ctttgctttt atcctcttta ttaaatggat ctacccaagc
      361 ctggatgaga taaggtattt tttcttcttt gctaaataca gaaatagatt tatcagcatt
      421 attacttaag taaaccaccc gcgtactacc aatgacaacg cctgcataag ttaatgctgg
      481 ttggcctaaa aaaacgctga gtataatcgc ggtaaataac ttacattaaa ctgttgtttt
      541 aaagagcctg attaaacaac tttttctgga cataacgact ccctggccaa agccaattga
      601 ataaaaaggc ccttgataaa ttatttctta tattcgacag taaattgtga atcagcgttc
      661 gccgtaccag ccgttacggt cgcattggtt gccacatagc gtgcaataaa cttcatttca
      721 gccttgcctt cagcatcgat agcggtataa tcagagtcgt tataaagttt aacctgcgaa
      781 ttatcggact ggttaaaaat acgaatacca acgccggttg ccgcaatcgc ttgtcctgta
      841 ccatcaaaac gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg
      901 aacttctgtg gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc
      961 agtgggacaa tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca
     1021 ccgacgaagt taacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc
     1081 agagtgaatg caccgcctat aatattacgc ttcataaaaa ctccattgat ataatatatc
     1141 ctttaccatg aatcatcact tatattaata agtgtatttt tgattcaaag gaatagtaca
     1201 ccaaagagac ggctttatct aaaaataata ttaattacgc aaaaataaaa tatagattta
     1261 ttgtttaaat attatttaaa cagtaaaaaa tcaagtttgt tatcagacat taatgataat
     1321 tcagaatata tttatataga aagatagatg aatttattga tgtcagttat aaaaaagggc
     1381 caccggt
//
LOCUS       DADLZM010000320         1339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLZM010000320 DADLZM010000000
VERSION     DADLZM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.327"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(7..489)
                     /locus_tag="JNY73_004268"
     CDS             complement(7..489)
                     /locus_tag="JNY73_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8050016.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDNARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(480..782)
                     /locus_tag="JNY73_004269"
     CDS             complement(480..782)
                     /locus_tag="JNY73_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8050017.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1109..1327
                     /locus_tag="JNY73_004270"
     CDS             1109..1327
                     /locus_tag="JNY73_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050018.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          393 a          334 c          286 g          326 t
ORIGIN      
        1 tcagactcag actttataaa acaaggtatt aggtgccaca actgactgaa caaaaccaaa
       61 acgctcataa aatgctctgg cgttatcatc aattgcatgt accataacag ccttaatgcc
      121 aacctgatca gccagattga caacccggtg aatggcatca cttagtagcc atttaccgaa
      181 accatgcccc tgggtacaaa catcaacagc cagacgccct aacagaacaa cagggacagg
      241 attgggcatg ttatgccgca aactgcgacc aagctcggca tgagtgacgg aaccagtagc
      301 taatgagtag tatcccacaa tacgtaaagt atcctccaag caaataacga acgtgcgaga
      361 agcattgagc atctggtttt tcatagcccg accacgtaac cagttactta gcacctcatt
      421 tccacagtca aatggctgaa gtatatgctc atccgtcaac aatgctggcg cggtgacatt
      481 tatttccatt caggctttac cgccatcaga cgttcacgcc cttctgcatt ctgcacagga
      541 gcttccagtt gcgtaataaa cgaatcataa cgttcgttat ccagcacaaa taaacgttga
      601 tccaggataa tgtttttagc ttcctggtac gccagttcca tgatgaaatc ggtacggttc
      661 ttattgacca agctcgcagc ataatcaata acggctcgtt cctcatcagt tgcccggatg
      721 ttaatctgat gttctcgcgg tgttttacgg acgcttgcag ctgtagacat aaaaacctcc
      781 attggtaaca atttgctatg caattaatgt tatcgcaaat ggtaacagaa cgctatacag
      841 aatcacttac gggaaaagcg atcccctgat aaaaacaatg gcagagacct gaacattgtg
      901 acgatgatct gtgtcggcgc acaaatcatg tctgattacg gccgtttaat gtgattttga
      961 taatgaaatg cctgaaccac ggcagcctgg cggctgcaaa tcggggtgtg gcagaatatg
     1021 agctttcaga gtaaccatcc agaatgagtt cccatgtcac acaacctgga gcaccagaaa
     1081 gtccacacca gaatggtgaa agagggttct gaaagccgtc gccagagcca ataatcaccc
     1141 ttataagtcc gtatttgcag actttatcac cggtcatccc tcctgcacgg tctgtttctg
     1201 ggaaaccttc cacaaaatgt acccggattc accgtacgaa tacgtcacgt tctgccatac
     1261 ctgccgtcgt tttgatttat acgaaacaga agcggagatg aaggcggatg acccgaagtg
     1321 gtggtgattt tcagccccg
//
LOCUS       DADLZM010000321         1286 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLZM010000321 DADLZM010000000
VERSION     DADLZM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.328"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..805)
                     /locus_tag="JNY73_004271"
     CDS             complement(<1..805)
                     /locus_tag="JNY73_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8050019.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="JNY73_004272"
                     /pseudo
     CDS             897..983
                     /locus_tag="JNY73_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(993..1073)
                     /locus_tag="JNY73_004273"
                     /pseudo
     CDS             complement(993..1073)
                     /locus_tag="JNY73_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          359 a          219 c          332 g          376 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata tattaatata
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccacagca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaaatg gttgattcat agtgactgga tatgttgtgt
     1201 tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat tgatattaat
     1261 gatttttact gtttctcggt cagctt
//
LOCUS       DADLZM010000322         1277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.329, whole genome shotgun sequence.
ACCESSION   DADLZM010000322 DADLZM010000000
VERSION     DADLZM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.329"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNY73_004274"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNY73_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY8050020.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          229 c          284 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DADLZM010000323         1272 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLZM010000323 DADLZM010000000
VERSION     DADLZM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.330"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            370..1119
                     /locus_tag="JNY73_004275"
     CDS             370..1119
                     /locus_tag="JNY73_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY8050021.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          360 a          257 c          255 g          400 t
ORIGIN      
        1 gctccttctg atgccattct atttcaggaa ggagtgtccg ttaaactcag gctacctcaa
       61 aaagttatcg gtgcgtttat cgaaaaacat atgttctact aattggagtc attgtctgta
      121 aaagcaactc aaaaatacaa gttgcttatt ccccttgttg gtaagaactt aattctaact
      181 gtttgttctg gatgttttaa acgtcctcct ttttatgctt gttcataacg gatatttaat
      241 aggtaacctt tgttttagca tcttctgatt tatttaaatt atgtgatagc gcacacaatt
      301 ttattatcca gtactttata ctcttaataa aataattata aatatttatc ctctataagg
      361 agatgttata tgaaaagaca gctttttact cttagtattg tcggggtatt ttctttaaat
      421 acctttgcat cattccctcc gggaaatgat gtgacaacaa aacctgacct ttactacctg
      481 acaaacgata atgctattga cagtctggca ttattaccgc ctccaccaca aatcggaagt
      541 attgccttcc tgaatgacca ggccatgtat gaaaaggggc gtttattgcg aaatactgaa
      601 agagggaagc tggcagctga ggatgccaat ctcagtagtg gtggcgtggc aaatgtattt
      661 tctgctgctt ttggttctcc gataacggct aaagattcac cagaattgca taaattactg
      721 acaaatatga ttgaggatgc cggagacctg gcaacgcgtt cagcaaaaga atattatatg
      781 cgtattcgac cttttgcttt ttatggtgtt tctacgtgta atacaaaaga acaggataca
      841 ttatccagaa atggctctta tccatcaggt catacatcga ttggttgggc aacagcactt
      901 gtattgtccg aaataaatcc ggcacgccag gataccattc tgaaacgggg ttatgaactg
      961 ggggacagca gggttatttg cggttatcac tggcaaagtg atgttgacgc agcacgtatt
     1021 gttggctctg ctattgtagc aacactgcac tcaaatcctg tgttccaggc acaattacaa
     1081 aaagcgaaag atgaattcgc taacaatcag aaaaaataat gcttttagcc agtacgcctt
     1141 ataaccccga ccttcagggc taatgccagt cagttaagca actgactggc tctttttcag
     1201 ggctgtgggg tatttccagg gcctctcctt taccactctc gggaaggccc tttcccttct
     1261 tgtcggtaat tt
//
LOCUS       DADLZM010000324         1226 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLZM010000324 DADLZM010000000
VERSION     DADLZM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.331"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..884
                     /locus_tag="JNY73_004276"
     CDS             <1..884
                     /locus_tag="JNY73_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8050022.1"
                     /translation="PTISLSAVIYFPPKFDETRQYPAIVVSHPGGGVKEQTAGTYVEK
                     LAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMG
                     ICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNART
                     SDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITY
                     DAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKA
                     YVAEAISVLAPFFEETL"
     gene            884..>1226
                     /locus_tag="JNY73_004277"
     CDS             884..>1226
                     /locus_tag="JNY73_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8050023.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          325 a          279 c          316 g          306 t
ORIGIN      
        1 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
       61 agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa
      121 cctatgtcga aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
      181 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg
      241 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
      301 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
      361 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg
      421 aaaacaatgt gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa
      481 ccagcgatat tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg
      541 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt
      601 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
      661 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
      721 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
      781 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
      841 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
      901 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
      961 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
     1021 gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg ttctcgctta
     1081 tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag atccgacaca
     1141 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttgatgc
     1201 tgaacgcatt ggcgtgctgg gtgtgt
//
LOCUS       DADLZM010000325         1210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLZM010000325 DADLZM010000000
VERSION     DADLZM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.332"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            238..1210
                     /locus_tag="JNY73_004278"
                     /pseudo
     CDS             238..1210
                     /locus_tag="JNY73_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013141603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          362 a          262 c          280 g          306 t
ORIGIN      
        1 ctgaatgtgg aggggctgaa gaaaaacgcg cgggatatca gtctgctgag gtatatgccg
       61 gcctgacaca ggctaagtgt gcccgggaat cggtgcaagt ataaaaaaca gcctgttgcg
      121 gtataagaat cgaccatgca gagagagaaa acaccggagt ggagggaaaa gcagaaaagc
      181 tcacgtggca taaggcgtgg acagcggtat cgtctggttt ttcaatttcc tatacgtgaa
      241 cgctatatag cccgattacg caacagagtt gaaaaccgtc tctggcattc actggcagct
      301 tgcattgatg actctcagac tcaacagctt ctggatcttc tttcggttcc tgccggaagc
      361 cgctattcgt tacttgatca attacgcgcc ggacctacta aagtgaatgc cacatcactg
      421 gtacaggcta taggtcgcct tcagacgata cgtagcctag gagtgacatt acctgcaatt
      481 acgccagttt cggatatccg tattgcggcc atggcacgtt atgcctcaac ggctaagata
      541 acagcgcttc agcgcttacc agaaaaaaga aaactggcta cgcttgttgc attctcttgc
      601 tgcatggagg cgacagctca ggatgatgct cttgagttac ttgaagcgct actccgggat
      661 ctgtttaatg aagcggttca agcagataaa cgtaaccgcc aacgaacact taaagatctg
      721 gatcgtgcag cagaaatact ggcaaaagca tgtcggatgc ttcttgacga taaattatct
      781 gatactgatg ttcgtgatag tatcttcaat atcattcccg aagatgtact gactcatgca
      841 gtaaataatg tgacatcaat tattcgacca gataataacg tttacttcaa tgaacttgat
      901 tctaaattca aaacggtgcg gcgtttctta cctgacttac tttccagaat acattttgag
      961 ggcaatgcct cagccaaaac attaattgaa gcgctttgct ggattgaagt taatttaaaa
     1021 aagaaaaaaa cagataacga tgcaccacgc gaaataatca ataaaccgtg gcaacagcat
     1081 gtgataagaa aagatggtag tattgatttc cacgcctata cattttgtgc acttaaggaa
     1141 cttcagcttg cactgaaaaa acgggatatt tatgtcaatc ccagctggcg ttatgcagat
     1201 ccccgggcag
//
LOCUS       DADLZM010000326         1210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLZM010000326 DADLZM010000000
VERSION     DADLZM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.333"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNY73_004279"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNY73_004279"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY8050024.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..>1210
                     /locus_tag="JNY73_004280"
     CDS             943..>1210
                     /locus_tag="JNY73_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050025.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSA"
BASE COUNT          280 a          327 c          297 g          306 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga
//
LOCUS       DADLZM010000327         1207 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLZM010000327 DADLZM010000000
VERSION     DADLZM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.334"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            14..925
                     /locus_tag="JNY73_004281"
     CDS             14..925
                     /locus_tag="JNY73_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8050026.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="JNY73_004282"
     CDS             941..1168
                     /locus_tag="JNY73_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050027.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          340 a          256 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggcaa
//
LOCUS       DADLZM010000328         1185 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLZM010000328 DADLZM010000000
VERSION     DADLZM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.335"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>1185
                     /gene="amn"
                     /locus_tag="JNY73_004283"
     CDS             <1..>1185
                     /gene="amn"
                     /locus_tag="JNY73_004283"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8050028.1"
                     /translation="AIGKYITIGELPDENARKQGLFVYPSLTVTWDGSTTNPPKTRAF
                     GRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPYVIDGSEL
                     TLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSLARLRHYT
                     GTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWITAETEAPEE
                     AISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDVWLMIGHC
                     GGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGE
                     EVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVPYGT
                     LLC"
BASE COUNT          287 a          312 c          276 g          310 t
ORIGIN      
        1 gccattggca aatatattac aattggcgaa ttacctgatg aaaacgcccg taaacaaggt
       61 ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaacaaa tccccccaaa
      121 acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat tactcgccct
      181 actctctttc gttcgtatct gaatgagcaa ctaacgttgc tttatcagga ttatggtgcg
      241 catatctctg tgcaaccctc gcaacatgaa atcccttatc cttatgtcat cgatggctct
      301 gaattgacac ttgatcgctc aatgagcgct gatttaactc gctacttccc gacaacagaa
      361 ctggcgcaaa tcggcgatga aactgcagac ggcatttatc atccaacgga attctccccg
      421 ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cacggttgcg ccattatacc
      481 ggtacgccag tagaacattt tcaaccgttc gtcttgttta ccaactacac acgttatgtg
      541 gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc ctacattgcc
      601 ctttcttgtg ctggcgggaa ctggatcact gccgaaaccg aagcgccaga agaagccatt
      661 tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac cgctgatggt
      721 cagggtatta ctctggtgaa tattggcgtg ggaccgtcca atgctaaaac catctgcgat
      781 catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg cggattacgt
      841 gaaagccagg ccattggaga ttatgtcctt gcacacgctt atttacgcga tgaccacgtt
      901 cttgatgcgg ttctgccgcc cgatatacct attccgagca ttgctgaagt gcaacgtgcg
      961 ctttatgacg caaccaagtt agtcagcggc aggcctggtg aggaagtcaa acagcggctg
     1021 cgtacaggta ctgtggtaac cacagatgac aggaactggg agttacgtta ctcagcttct
     1081 gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc aaccattgcc
     1141 gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgt
//
LOCUS       DADLZM010000329         1178 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.336, whole genome shotgun sequence.
ACCESSION   DADLZM010000329 DADLZM010000000
VERSION     DADLZM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.336"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..332
                     /locus_tag="JNY73_004284"
     CDS             <1..332
                     /locus_tag="JNY73_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8050029.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
     gene            332..679
                     /gene="tnpB"
                     /locus_tag="JNY73_004285"
     CDS             332..679
                     /gene="tnpB"
                     /locus_tag="JNY73_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8050030.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            699..>1178
                     /locus_tag="JNY73_004286"
     CDS             699..>1178
                     /locus_tag="JNY73_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8050031.1"
                     /translation="MDTSLSHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     "
BASE COUNT          281 a          327 c          321 g          249 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag cagataaata caccgtccga cagtctgtgc tgtgagctgg
      241 ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta ttacagatac
      301 ttatccgcga aataaaaggg agcagccact gatgatatct ttccctgcag gttcgcgtat
      361 ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg cctcaaaagt
      421 tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc gcggacgccg
      481 gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct ttaccagacg
      541 cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc accttactcc
      601 ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac gaacggaacg
      661 cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca ctttctcatg
      721 agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc cagatggcga
      781 aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc aaccggctga
      841 aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa aaacttcgcg
      901 caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt caggaagaaa
      961 tggcggaaac gctgggtgag caatatgacc cggtactgcc atctcccctg cgccagtctt
     1021 cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc cgtccggaag
     1081 aggaatgctg cccggcctgt ggtggcgaac tcagttctct gggatgtgat gtgtcagagc
     1141 agctggagct tatcagcagc gcctttaagg ttatcgaa
//
LOCUS       DADLZM010000330         1158 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLZM010000330 DADLZM010000000
VERSION     DADLZM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.337"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..387
                     /locus_tag="JNY73_004287"
     CDS             <1..387
                     /locus_tag="JNY73_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050032.1"
                     /translation="LVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(388..1032)
                     /locus_tag="JNY73_004288"
     CDS             complement(388..1032)
                     /locus_tag="JNY73_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8050033.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          263 c          278 g          322 t
ORIGIN      
        1 ctggtgctgg tggcaattct gattatgctg ccgactctcg gcatcggtga tatgaacaat
       61 ctctataaat ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta
      121 tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt
      181 attcgtaata agcctctggc aatgaccgtc gggatttggt gttttgcctt taccgcctgt
      241 gcctgtttga cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag
      301 ttggcgctga atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg
      361 ctggcgcgta aagcgaatag caaataatta ttgtggtcgg tgacgctgac tctggcagat
      421 ctgacccgtt tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt
      481 tgggtaatag acttggaagc ctggacgctc tgtgatccag tcttccagta cacattctaa
      541 acgaccagta tcaaggtact ccttgatctg ttcatagagc agatacccga taccagcacc
      601 catcaaaacg gcctccagtt cagcatcaac atcatccaga atgatatttc ctgcgacagc
      661 aatttccagc tcctttgcaa attgccagtg gaaaggtttg ccgctgggat aacgaaaaac
      721 aacacattga tgattaagca gatcatgagg atggcggggt ttgccatatc ttgcaaagta
      781 ctctggcgtt gctgctacac ataatttcac tggtgggccg atcgccacgc tgatcatatc
      841 tttttcgacg atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat
      901 aagagaatcg tcagttgtga gctctacttt aatatcagga tattcacgtg tgaaaccaac
      961 cagtaaaggc attaaaaata tacgtgcagc tacccgggcg gcattgattt tgagcgttcc
     1021 tgtcggtgtc agacgaaaat cattcatctc atctaacatg atctgaattt cgtcgaaagc
     1081 tgggcgcaat cgttcataga gattagaacc cgcttccgtt aacgatacgc ttcgggttgt
     1141 ccgattgaat aagcgaat
//
LOCUS       DADLZM010000331         1154 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLZM010000331 DADLZM010000000
VERSION     DADLZM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.338"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(569..781)
                     /locus_tag="JNY73_004289"
     CDS             complement(569..781)
                     /locus_tag="JNY73_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050034.1"
                     /translation="MVVWRFGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINL
                     LDKSDYVLAHGLNTDDQLAVGIVYQF"
     gene            841..>1154
                     /locus_tag="JNY73_004290"
     CDS             841..>1154
                     /locus_tag="JNY73_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8050035.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGR"
BASE COUNT          318 a          213 c          298 g          325 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc cccgaatctc cagacaacca
      781 atatcactta tttaagtgat agtcttaata ctagttttta gactagtcat tggagagcag
      841 atgattgatg tcttaggacc ggagaaacgc agacggcgta ctacacagga aaagatcgct
      901 attgttcagc agagttttga accgggaatg acggtctccc ttgttgcccg gcaacacggt
      961 gtggcagcca gccagttatt tctctggcgc aagcaatacc aggagggaag tcttactgct
     1021 gtggccgcag gagaacaggt cgttcctgcc tctgaacttg ctgctgccat gaagcagatt
     1081 aaagaactcc agcgcctgct cggcaaaaaa acgatggaaa atgaactcct taaagaagcc
     1141 gttgaatatg ggcg
//
LOCUS       DADLZM010000332         1150 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLZM010000332 DADLZM010000000
VERSION     DADLZM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.339"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..232)
                     /locus_tag="JNY73_004291"
     CDS             complement(<1..232)
                     /locus_tag="JNY73_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8050036.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQ"
     gene            complement(499..1005)
                     /locus_tag="JNY73_004292"
     CDS             complement(499..1005)
                     /locus_tag="JNY73_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050037.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQSDLSQGDQLTEMLSFLTSEEREAL
                     TNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEASILS
                     ESVNAKAG"
BASE COUNT          296 a          265 c          242 g          347 t
ORIGIN      
        1 gttggctggc tgcaaccgct aatgggctgg aaccgaggtt gccgatttgc acgtcgccag
       61 aagccagcgc ccgcacgatg ctggctccgc tgtcaaactt acgccagtcc acgctcgccc
      121 cgctctcttt agcaaaggtg ttgtcggcct gagccacttt cgccggttcg gctgaggttt
      181 gatacgccac ggtgacgttc accgcctgtg cctgaaaagc gatgaatgcc agtgcggcaa
      241 gaagtgtgtt atatgactag gtaatttatt caagataacc gggaatgggt aaaaaaagac
      301 gggatttgat gggattcaat aagttatgcg ccgattgcat tcaagataaa acgagttaaa
      361 aatgctcaat gcttcgatat cgataaaaca ttcaagatag gcgcgccgtg tattttaatt
      421 cttttaagac tcttcaaaaa tgtcatcgta gcctttaaaa acgaatttta aaggcaccct
      481 aaaggcaggt caatgaagtt aacccgcctt cgcgtttaca ctctctgaga gaatactagc
      541 ttcaatctct tccaaaatct ctctgacttg ctcgtcgggc atatcaacga gcatcaacgc
      601 tgccaggcgt ttacggccct ctagttgcag tagctcttga gtttccttat ctaacaagcg
      661 tagcacagtc tcaacacctt tccttgcgag catgtttgta agggcttctc gttcctcact
      721 ggtaagaaac gacaacattt ctgttagctg atccccttgg cttaaatcac tttgtgtttt
      781 tggtggattc ccgttaccgg tttttagcca ttcaagacta acaccttcac tttctgcaat
      841 gcgtaccaat atgctttctc gaggttctgg tgtatttccc tttttaaaat agctttgtaa
      901 cgtattgata tttatgccac aggctctggc ggcagcactt cttgatggat accgcctaat
      961 aagataagcc atgcgctcca cgataggttc ttttgcctgc tccataaaag tacctctagg
     1021 tatttttctg tataaaggtt cttttccatc tatctaattg aatggaaagg taattttaaa
     1081 aatccaaata aaatacaaaa aaataccttg tattggtgct ttttgtgatc tgcgatttta
     1141 tttactcgga
//
LOCUS       DADLZM010000333         1126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLZM010000333 DADLZM010000000
VERSION     DADLZM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.340"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            252..1007
                     /gene="mlaA"
                     /locus_tag="JNY73_004293"
     CDS             252..1007
                     /gene="mlaA"
                     /locus_tag="JNY73_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY8050038.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          276 a          260 c          293 g          297 t
ORIGIN      
        1 gcttttttgc tcgcaactgg cgggagttac tctgaaaatg tagaaaaggt tgcgtttgcc
       61 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
      121 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
      181 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
      241 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
      301 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc
      361 gcaccatgta caacttcaac ttcaacgtat tagacccgta tattgttcga ccggtcgctg
      421 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaatgg tttgagcaac tttactggca
      481 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
      541 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
      601 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
      661 ttggtcatta tggcgtgggt tatgggcctt acgttcagtt accgttctac ggtagcttca
      721 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
      781 cctggccgat gtctgtgggt aaatggacgc ttgaagggat cgaaacccgc gctcagctgc
      841 tggattccga tggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
      901 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
      961 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa
     1021 aaggtgagtc gcaaaactca ccttttttgt tgttatccct caattaccac aagtaaaagg
     1081 agataaaccg acaatgaccc ggacgtaatg atgtaaaaat actgat
//
LOCUS       DADLZM010000334         1120 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLZM010000334 DADLZM010000000
VERSION     DADLZM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.341"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..1120
                     /gene="pdeF"
                     /locus_tag="JNY73_004294"
                     /pseudo
     CDS             1..1120
                     /gene="pdeF"
                     /locus_tag="JNY73_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT          245 a          254 c          250 g          371 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt ggggcttccg ctgttcctgc cttctttaat
       61 cttgcccatt ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct
      121 cttttatctg cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt
      181 gccagggatc accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac
      241 gctctcatta acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt
      301 ctttactccc cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat
      361 attctggcag atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt
      421 tgtaggatta ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac
      481 tttaatcaat tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt
      541 catcattcgg gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca
      601 gcaggttgat gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc
      661 tttattgttg ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta
      721 taccttgtca ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct
      781 gatttcgctg ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat
      841 tcccatttat cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt
      901 ctcttttatt gtcaattata tggctgtact ggcaactcgt cagcgagcgg tagtcagacg
      961 cattcagcgg cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa
     1021 tcgcgcgttg cgtgatgccc tctggtctgc gctttgttat ttacgcatcc ctggcatgga
     1081 aatgctggtt aagaactatg gcatcatgct gcggattcaa
//
LOCUS       DADLZM010000335         1110 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLZM010000335 DADLZM010000000
VERSION     DADLZM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.342"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..1019
                     /locus_tag="JNY73_004295"
                     /pseudo
     CDS             1..1019
                     /locus_tag="JNY73_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1110
                     /locus_tag="JNY73_004296"
     CDS             992..>1110
                     /locus_tag="JNY73_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY8050039.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          316 a          270 c          255 g          269 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctagacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcatt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DADLZM010000336         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.343, whole genome shotgun sequence.
ACCESSION   DADLZM010000336 DADLZM010000000
VERSION     DADLZM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.343"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..387)
                     /locus_tag="JNY73_004297"
     CDS             complement(<1..387)
                     /locus_tag="JNY73_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8050040.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="JNY73_004298"
     CDS             816..1097
                     /locus_tag="JNY73_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050041.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          307 a          249 c          252 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctgc ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DADLZM010000337         1105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLZM010000337 DADLZM010000000
VERSION     DADLZM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.344"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..160
                     /gene="ospE1"
                     /locus_tag="JNY73_004299"
     CDS             <1..160
                     /gene="ospE1"
                     /locus_tag="JNY73_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY8050042.1"
                     /translation="TVFNKKAAIYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEK
                     QSQRYSDF"
     gene            549..>1105
                     /locus_tag="JNY73_004300"
     CDS             549..>1105
                     /locus_tag="JNY73_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8050043.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          399 a          211 c          183 g          312 t
ORIGIN      
        1 tactgttttc aataaaaaag cagctatata cgaattacaa atacctgctg ccagccggac
       61 gaaaacgttg aaattcactg ctacagaaat gcaatggttg acaaagataa atgaagccgg
      121 aattgatgaa aaacaaagcc aacggtattc tgatttctga agttaatttc agactcgttt
      181 aagctccatt ttcaaaaaaa tatatcaggg tttgttacta ataacttctg aaaacagaaa
      241 aaaatagagc agtaattcta tttcagagag gagtaactcc atctatcctc gatacgtact
      301 gaacgttatc gtgatatatt catcatgctt tcttcaccat tattcgagta tagggagaga
      361 cgcaatgacc ttaaatacct ttttatggct gaattaccat tctcaggaat aacagcagac
      421 tccttcccat agacggctta gggggaaagc aatacccgga gaaagggtac aaagcaaact
      481 gttttaaaat taataagcca tattctattt cactagaatt cttatcgttg ataagaattc
      541 tggttataat gattaaatca accaatatac aggcaatcgg ttctggcatt atgcatcaaa
      601 taaacaatat atactcgtta actccattat ctttacctat ggaactgact ccatcttgta
      661 atgaatttta tttaaaagtc tggagcgaat gggaaaagaa cggtacccca ggcgagcaac
      721 gcaatatcgc cttcaatagg ctgaaaatat gtttacaaaa tcaagaggca gaattaaatt
      781 tatctgagtt agatttaaaa acattaccag atttaccgcc tcagataaca acactggaaa
      841 taagaaaaaa cctattaaca catctccctg atttaccacc aatgcttaag gtaatacatg
      901 ctcaatttaa tcaactggaa agcttacctg ccttacccga gacgttagaa gagcttaatg
      961 cgggtgataa caagataaaa gaattaccat ttcttcctga aaatctaact catttacggg
     1021 ttcataataa ccgattgcat attctgccac tattgccacc ggaactaaaa ttactggtag
     1081 tttctggaaa cagattagac agcat
//
LOCUS       DADLZM010000338         1102 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLZM010000338 DADLZM010000000
VERSION     DADLZM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.345"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            52..>1102
                     /locus_tag="JNY73_004301"
     CDS             join(52..301,301..>1102)
                     /locus_tag="JNY73_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HAY8050044.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAIT"
BASE COUNT          303 a          273 c          310 g          216 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gggccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg ga
//
LOCUS       DADLZM010000339         1100 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLZM010000339 DADLZM010000000
VERSION     DADLZM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.346"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(257..997)
                     /locus_tag="JNY73_004302"
     CDS             complement(257..997)
                     /locus_tag="JNY73_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY8050045.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT          304 a          233 c          237 g          326 t
ORIGIN      
        1 cacattgcat gtggtgaacg gaaccgcccc gagtttccta gagaatgttt tatctgtgaa
       61 ctcaggttgc cagatcatcg tttccgatgg cagcataata agctttttct gcttctgccg
      121 gagggatatg tcccagcctt tccagtaatc ccttaaattc aggggtaaaa cgtggtgtgc
      181 ccatagactc ctcctatgct catatagggt agatttgtct atgggccctc catagcctga
      241 tacaggctgt ccagctttat ggggtcagtt cattggtagg agtatttaat tcttcaaact
      301 cttttttggc ttcgctaagg cttttggtaa attcaggtgt attatgaagt actgcaacaa
      361 ccgatgctcc cattaaacgc ccagcctcta catcgctttg ccaatgcgca ccgcagatga
      421 cccgactttc tccaaactca tatccacgtc gaagtatttc cgctttacgt tgaggattaa
      481 tctccgcaag tatcagtgct actgcccaac caaaggatgc atgaccagag ggataagagc
      541 cagtgatagc cattttctca tctttatcag gtgtacaggt tgcgtcttta taaataacga
      601 atggacgaac tcttttataa tattctttgg cgttacgcac ggcgtaatca tggctatcct
      661 gtagtacttg tgttaacaac tgatataaga ttggggtatt ttcccttgaa atatccatgc
      721 cataagcatc tgaaaatgca agaccaaaat tctcgtagta tgcatcttcg ctagcgagac
      781 gtacacgatt agcatcccgg agcgagcggc cgaattcata atgagctttg tcagcctgaa
      841 atactactga atcctctgcc ggaggcggcg gaagtattga caaactgtct ggtgaagttt
      901 gttgagtgag aaaaccttct gccttcagag catttgctga ggggataaaa atcatatttg
      961 tagcaataca aaaaagaaga aagtttttgg ttttcataat ttatttcctt agcataatta
     1021 attgatgatt ttggtcagct gtaaaaaacg agctgtgtta caaaaaacac aagtcatgcg
     1081 gaaaaatttg aacagagtga
//
LOCUS       DADLZM010000340         1078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLZM010000340 DADLZM010000000
VERSION     DADLZM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.347"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..276
                     /locus_tag="JNY73_004303"
     CDS             <1..276
                     /locus_tag="JNY73_004303"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8050046.1"
                     /translation="KKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSK
                     QQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
     gene            273..>1078
                     /locus_tag="JNY73_004304"
     CDS             273..>1078
                     /locus_tag="JNY73_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HAY8050047.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
                     SPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
                     GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSM
                     SAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWR"
BASE COUNT          267 a          280 c          306 g          225 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
      361 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      421 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
      481 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc
      541 gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcagc taccgcgcac
      601 acggcctgcc tgtgtcagaa aatctgttgg agcaggattt ttacgccagt ggcccgaacc
      661 agaagtggcc aggagacatc acgtacttac gtacagatga aggctggctg tatctggcag
      721 tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg
      781 cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg ccccggaacg
      841 ttatcgttca cacggaccgt ggaggccagt actgttcagc agattatcag gcgcaactga
      901 aacggcataa tctgcgtgga agtatgagcg caaaaggttg ctgctacgat aatgcctgcg
      961 tggaaagctt ctttcattcg ctgaaagtgg aatgtatcca tggagaacac tttatcagcc
     1021 gggaaataat gcgggcaacg gtgtttaatt atatcgaatg tgattacaat cggtggcg
//
LOCUS       DADLZM010000341         1071 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLZM010000341 DADLZM010000000
VERSION     DADLZM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.348"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..767
                     /locus_tag="JNY73_004305"
     CDS             <1..767
                     /locus_tag="JNY73_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8050048.1"
                     /translation="FATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            788..1069
                     /locus_tag="JNY73_004306"
     CDS             788..1069
                     /locus_tag="JNY73_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY8050049.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     CWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQSVNRPGNPGD"
BASE COUNT          346 a          239 c          244 g          242 t
ORIGIN      
        1 aatttgcaac agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa
       61 aaaagtccaa gatagaacca gatcccctca cccgtgacga atttattcgt tttattgagg
      121 cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc
      181 acggggagct ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca
      241 tccgccggaa ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg
      301 gaagaacgat tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga
      361 ccatgcttgg aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg
      421 tgcataaatg cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac
      481 cgcactataa ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc
      541 tgagacacag aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag
      601 gagctaatcc tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca
      661 atgtttacgg agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa
      721 gactatctga aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc
      781 aaaatcaatt ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg
      841 attgctgaaa ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga
      901 ccggatccgc gagatcaaat gctggatcga caacggcgtg caggtcagca aagttaaaat
      961 actgctcagt aatgaaaatg ttgatgtgca gaacgtctgg cgcgatcagc aagaaacatt
     1021 actgacttac ctgcaaagcg tgaaccgccc cgggaatcct ggagactaaa c
//
LOCUS       DADLZM010000342         1036 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLZM010000342 DADLZM010000000
VERSION     DADLZM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.349"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..280)
                     /locus_tag="JNY73_004307"
     CDS             complement(<1..280)
                     /locus_tag="JNY73_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HAY8050050.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQ"
     gene            complement(300..>1036)
                     /locus_tag="JNY73_004308"
     CDS             complement(300..>1036)
                     /locus_tag="JNY73_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY8050051.1"
                     /translation="QLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQ
                     DDIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGP
                     GCTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQ
                     HENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLIL
                     ALSFWKLRRKKADKIYILLMLILNIF"
BASE COUNT          372 a          184 c          123 g          357 t
ORIGIN      
        1 gttgcacatc aaacagtata tcatttattt tattaccagc taataagccg acaaagaagc
       61 cataaaaagg aaataaaagc aaccatataa aaagactgac acatttttgg gtgattgaaa
      121 aattgaatgt aactaaatta aaaattaaaa gtataaaact aaatacaaat aataaatatc
      181 gcaaagggaa tattcccaat ggctccagta atcttccacc accaccaata atcagttcaa
      241 tacataaaaa tgttataaaa attttattta tattattcat attcgtaagg tgatgttttt
      301 taaaatatgt ttaaaatcaa cattaataaa atataaattt tatcagcctt tttccgcctt
      361 aacttccaaa aacttaaagc caagatcaaa ttgcctatat aacctttacc caattgatta
      421 ttcacaaagc tatcatcagc taaggcatta gctattttag ttacttcttt tcgataccac
      481 ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt tgctcccact
      541 tggttgtttt catgctgtct gtacagcatt gttggttttc tatcaatata ccacgaataa
      601 tttctcgtcc gcgcaaatga atataaaaac caatcatgca gacagatatc ttgtgcagca
      661 tttttattat ttatgatgaa cttcttaaat tcaataagcg tttctttttt tataacatat
      721 gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga ctgagcctta
      781 tcaactaaat ttttttgccc tgatggataa tatgcaatta cattgctgga ataacaattc
      841 gcattattaa ataccaagca gtcaattgca ttttttattt tatctttata ccaaatatcg
      901 tcctgatcac taaaacagat ataatcatag ttttcaagat cgacatcttt aattaatcta
      961 taaaaatttt tacctgcccc cccaaactta ttgacgcttg gaagtatatt tataattgag
     1021 ggatatgata attgaa
//
LOCUS       DADLZM010000343         1031 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLZM010000343 DADLZM010000000
VERSION     DADLZM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.350"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..573
                     /locus_tag="JNY73_004309"
     CDS             <1..573
                     /locus_tag="JNY73_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HAY8050052.1"
                     /translation="RHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFHQQRNPVID
                     YVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKA
                     TLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMIARQMNKTV
                     EILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(727..>1031)
                     /locus_tag="JNY73_004310"
     CDS             complement(727..>1031)
                     /locus_tag="JNY73_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY8050053.1"
                     /translation="KRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQP
                     VYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          265 a          227 c          244 g          295 t
ORIGIN      
        1 cggcacaata acagaaatgt gatttttctg gttccccatg gctggagcgt ggatattcct
       61 gcaatgttgc tggcagctca gggggaaaaa atggccgcca tgtttcatca gcaacgaaat
      121 ccagtgattg attatgtctg gaattctgta cggcgtaaat tcggggggcg cttacattcc
      181 cgggaggatg ggataaaacc atttattcag tcagtacgcc agggatactg ggggtattac
      241 cttccagatc aggatcatgg tcctgaatac agtgaatttg ctgatttttt tgcgacctat
      301 aaagcgacat taccaattat tggacgtctg atgaacatca gtcaggctat gattataccg
      361 cttttcccgg tttatgatga aaaaaaacat ttcctgacta ttgaagttcg gccaccaatg
      421 gatgcatgca ttgccagcgc ggacaataaa atgattgccc gacaaatgaa caaaacagtg
      481 gaaattttgg tggggtcaca tccggaacag tatatctggg ttttaaaatt gttaaaaacg
      541 cgcaaatcaa acgaagcgga cccgtaccct tgaaattata aagtacaggt atttccacta
      601 gttgtttctt acaggttacc aatcgaaaca catccccctt ccgtgtgttg cccgtttggc
      661 acggaggact gtgtagatgc agttcgttaa atttaattta tgcctaaata gctgcgctta
      721 ataccgctac acttttgcca gcccatgttt gcctccgggg aatgggctga cggtttccat
      781 aaaatggcga acttttttca acagttgcca cattgagcgg cactgatgat tacgcgttat
      841 tgtgtcgtga agtgcctgcc atagccgttc aacatgattc acccatggcg agtaaaccgg
      901 ctgataaatg cccctgaact tcgggttctc cttcagccag ctctgtgttt cccggctttt
      961 gtggataatg tagttgtcca cgatcagcgt gatggttttt gcccgacggt atgtcgcttt
     1021 aagccgcttc g
//
LOCUS       DADLZM010000344         1030 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLZM010000344 DADLZM010000000
VERSION     DADLZM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.351"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..213
                     /locus_tag="JNY73_004311"
     CDS             <1..213
                     /locus_tag="JNY73_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8050054.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="JNY73_004312"
                     /pseudo
     CDS             356..577
                     /locus_tag="JNY73_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(598..660)
                     /locus_tag="JNY73_004313"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="JNY73_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          331 a          226 c          215 g          258 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg cgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact
//
LOCUS       DADLZM010000345         1027 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLZM010000345 DADLZM010000000
VERSION     DADLZM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.352"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="JNY73_004314"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="JNY73_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY8050055.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          333 a          151 c          176 g          367 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaa
//
LOCUS       DADLZM010000346         1025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLZM010000346 DADLZM010000000
VERSION     DADLZM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.353"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>1025)
                     /locus_tag="JNY73_004315"
     CDS             complement(<1..>1025)
                     /locus_tag="JNY73_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8050056.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPY
                     VNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKL
                     GEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGT
                     PVNGYFL"
BASE COUNT          303 a          268 c          219 g          235 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gagaatacca
      121 gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa gtattaccca
      181 tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttcgtc attaagtcat
      241 ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga atggcacaaa
      301 ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct gggttgcgcg
      361 tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc agcattgaaa
      421 tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta atgtttgtgg
      481 tcgcaatatg cacttcagta atcgcaggcg cggttaccgc catcgccaca tacaggagcg
      541 acataacaaa catcgcaatc cctgccacag aaccgacgat ttttagcgat ttcataccgc
      601 gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt aacgctacga
      661 ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc gcaatcagaa
      721 ttgcctgggg tttttgtgcc agatagggaa tatgcaccac ccagtaggtc cacgcggcga
      781 gataagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg cccttcccat
      841 ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa tagagtgcaa
      901 agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta ttaacaacgt
      961 tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat cgcaactgtt
     1021 tttcc
//
LOCUS       DADLZM010000347         1022 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.354, whole genome shotgun sequence.
ACCESSION   DADLZM010000347 DADLZM010000000
VERSION     DADLZM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.354"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(115..984)
                     /locus_tag="JNY73_004316"
     CDS             complement(115..984)
                     /locus_tag="JNY73_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAY8050057.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          259 a          301 c          216 g          246 t
ORIGIN      
        1 caaaacgata gtacgcattt ctgaatggaa tattcagtgc cataattacc tgcctgtcgt
       61 taaaaaattc acgtcctatt tagagataag agcgacttcg ccgtttactt ctcactattc
      121 cagttcttgt cgacatggca gcgctgtcat tgcccctttc gccgttactg caagcgctcc
      181 gcaacgttga gcgagatcga taattcgtcg catttctctc tcatctgtag ataatcccgt
      241 agaggacaga cctgtgagta acccggcaac gaacgcatct cccgcccccg tgctatcgac
      301 acaattcaca gacattccag caaaatggtg aacttgtcct cgataacaga ccaccacccc
      361 ttctgcacct ttagtcacca acagcatggc gatctcatac tcttttgcca gggcgcatat
      421 atcctgatcg ttctgtgttt ttccactgat aagtcgccat tcttcttccg agagcttgac
      481 gacatccgcc agttgtagcg cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca
      541 tagatcttca cgaatattag gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt
      601 catcgcagta aatgcgctgg tacgcgaagg ctcggcagac aacgcaattg aacagagatg
      661 taaccattcg ccatgtcgcc agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact
      721 ggggcggacc ataaacgtaa atgaacgttc cccttgatcg ttcagatcga caagcaccgt
      781 ggatgtccgg tgccattcat cttgcttcag atacgtgata tcgactccct cagttagcag
      841 cgttctttgc attaacgccc caaaaggatc atcaccgacc cgacctataa acccacttat
      901 tccgcctaat ctggcgattc ccaccgcaac gttagctggc gcgccgccag gacaaggcag
      961 taggcgcccg tctgattctg gcaagagatc tacgaccgca tcccctaaaa cccatacttt
     1021 gg
//
LOCUS       DADLZM010000348         1016 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLZM010000348 DADLZM010000000
VERSION     DADLZM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.355"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..273
                     /locus_tag="JNY73_004317"
     CDS             <1..273
                     /locus_tag="JNY73_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8050058.1"
                     /translation="IRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSH
                     ADTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            275..>1016
                     /locus_tag="JNY73_004318"
     CDS             275..>1016
                     /locus_tag="JNY73_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050059.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTV"
BASE COUNT          323 a          186 c          200 g          307 t
ORIGIN      
        1 atccgtagaa atgatggttc gatccgcttt aacaataaga tgccttatca cgttacgctg
       61 ggttatatcg gaacaaacgg aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc
      121 ccttatagcc atgcagatac gcagtttaaa aatgttccgt cagcttttca ggttggttat
      181 atcaatgatt ttggtggatt aagtttttat gaaataaact gcccgacggt taataactct
      241 tgtaatgttt ccgtagccaa acgagataaa taatatgcgc tacttattaa ttgtaattat
      301 atttattata ggttttagtg cattacctgt atgggcaatg gactgctatg ctgaacatga
      361 agagggaaat accgtagtca ttggttacgt accaagaatt tctatcccca gtaatggtaa
      421 aaaaggtgat aagatctggc aaagcagtga gtattttatg aatgtcttct gtaataatgc
      481 attacctgcc ccatcacctg gagaagaata cccatcagcc tggactaata taatgatgtt
      541 tttaccaggc ggtcaggact tttataatca gaactcctat atttttggcg taacatacaa
      601 tggagttgat tatgattcga ctgcccctaa tgctatcgca gcaccagaat gcattgatat
      661 aaaaggagcg ggtaccttta ataatcacta taaaaacccc gctgtctgta gtggaggtcc
      721 tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa ctctatatta aattagcaaa
      781 aaatgccaat agagttaata aaaacctggt gttacctgat gaatatatcg ctccagagtt
      841 taaaggtatg agtggcgcag gagctataga agtagacaaa aatttgacct tcaggattcg
      901 tggattaaat aatattcatg tccttgactg ctttgttaat gtcgcactgg aaccagctga
      961 tggcgttgtt gactttggta aaataaattc ccgaacaatt aaaaatacca ccgtta
//
LOCUS       DADLZM010000349         1000 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLZM010000349 DADLZM010000000
VERSION     DADLZM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.356"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..372)
                     /locus_tag="JNY73_004319"
     CDS             complement(<1..372)
                     /locus_tag="JNY73_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8050060.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVTRQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKIN"
     gene            complement(372..587)
                     /locus_tag="JNY73_004320"
     CDS             complement(372..587)
                     /locus_tag="JNY73_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8050061.1"
                     /translation="MYQMEKISTGIAYGTSGGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          269 a          260 c          206 g          265 t
ORIGIN      
        1 gtttattttg cgaagaagcg tcgatgttct gaaattgcca gcgcccacgt tgtaaacgaa
       61 cgagtaaaga gcgccgcgcg ttgtttccgg tatatcgact ttgatgtacg ggttaatttg
      121 tctggtgaca gtgacaaggt ctttattcag gagagctttg cattctgctt cggtatacgt
      181 tttaccaggc atgatgtctt ttccggtgtg tccgtgacat acagtccata caccaatgat
      241 atctttgtat ggtatgtagc tgacaccttc cagaccatcg ttaccacctg ggccagtgat
      301 taacacagat gctatagcaa cagccccgcc accaatagca actgcaacag ccttgcgtaa
      361 tgatggcgac attattcacc tctcgcagcc ttacgcttat cttctcttat tttgaaatac
      421 agattcgtca gataagtcag aagccccaga agcagacttc ccagcacacc aatcgcagcc
      481 cactgtgatg gactgacctg atccaaccac tgcaaaaacc agtagccggc actgccgccg
      541 gaggtgccgt aggcaatgcc tgttgatatt ttttccatct gatacatatc ccgccccccc
      601 cgacagacct gtgctatcgg aaagaaaaaa ggccatcagc aaaactctga tggcctgaat
      661 cacctttacc aatattgtat gaaaaaacac gcacgactaa ttgacaataa ttttcatttc
      721 cattaaaaaa gatcgcgtag cattcttaat tcatgaggaa ctttacccgc cagcaatctg
      781 agtagcgaaa gctgttcatc cccaatgttt ttgctggcgg gtcctttttt ctacggtcct
      841 cctccccgga ggggggcatt tcattatttt tctaatcgtt actggtgaga aggcggaaga
      901 aaaagccagt tcttcgcccc taaataacag catgattcca gtccatgcaa aatctgattt
      961 ccagctcaca cataccgagc gctgtacaaa aaaccgccag
//
LOCUS       DADLZM010000350          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLZM010000350 DADLZM010000000
VERSION     DADLZM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.357"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNY73_004321"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNY73_004321"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY8050062.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGTENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDHLVSPEGLAPEWD"
BASE COUNT          259 a          218 c          221 g          285 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggt
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag tgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADLZM010000351          977 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLZM010000351 DADLZM010000000
VERSION     DADLZM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.358"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            87..977
                     /locus_tag="JNY73_004322"
                     /pseudo
     CDS             87..977
                     /locus_tag="JNY73_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT          258 a          229 c          242 g          248 t
ORIGIN      
        1 gttgtttact gtgcgctgtt gtccatgtga tattgtgtac cagatgtgtg ccaaactatt
       61 atttctgagc gtaccaaata ttatttatgg ctataagcga cacaaaactg cgtactattt
      121 atggtaaacc atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa
      181 tttcaccgaa gggggtcatc cagttccagt accgctatcg ctggcatggc aagcctaatc
      241 gacttgggct tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg
      301 acttgcgaaa gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg
      361 agaactccat gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa
      421 ctctcagaga aaaggcctag gcactttatc gatcaacggt tatgaagcgc atgcatgacg
      481 ccttccctaa tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg
      541 aagaagaaaa agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa
      601 ttagttggtg catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa
      661 gggacttcgg ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta
      721 agatttggct tgctattgaa agaagccgtg cgtctacgtc aaataagctc cttcatcaga
      781 tgcttatgct gtggggggcg aggctctcag agcttaggct ggcaacaaag acagaatttg
      841 acctgctgga caacgtatgg accgtaccga aagagcatag caagatgggt aatgttatcc
      901 gccgtccaat cttcgaacaa attaagcctt ttctcgaaaa ggccatgaca atgtacaatg
      961 atgttctttt ccctgga
//
LOCUS       DADLZM010000352          934 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLZM010000352 DADLZM010000000
VERSION     DADLZM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.359"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            450..>934
                     /locus_tag="JNY73_004323"
     CDS             450..>934
                     /locus_tag="JNY73_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY8050063.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     V"
BASE COUNT          259 a          168 c          226 g          281 t
ORIGIN      
        1 tatgcaagaa cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt tgaatgaaaa
       61 tcagccggag atgattttac ataattgcta cggaattatt caatacagga attgcttgcg
      121 tctgcatgga ttgacctgaa atattcccga aaatttctct aaaaaactcg aaaaaaatgg
      181 taactggttg aatgtattaa tatgcaatgg tacgtgtcag ggattaaaag atgaacgtaa
      241 atttattcaa cgcattaatt ttaaagggtt ttattgtttg ttgacgaaaa caggaatcat
      301 gttcggtctc tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca
      361 accataacac acactctact gtctgagtcc agcgtttttt aacattcttg ttaagattat
      421 gtgatcttta gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa
      481 ttgcgggtca tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc
      541 agttgcgccg caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag
      601 tgggagacgt tatcgatcgt gggccgcaaa gtttacgttg tctgcagtta ctggaacaac
      661 attgggttcg tgcggtaaga ggcaatcatg aacagatggc gatggatgcg ctggcattcc
      721 agcagatgtc tttgtggttg atgaatggcg gcgactggtt tattgcgctg gcagataatc
      781 aacagaaaca agcgaaaacg gcgctggaaa aatgtcagca tttgcccttt attcttgaag
      841 tacacagtcg caccggcaag catgttattg ctcatgccga ttatccagat gatgtttatg
      901 aatggcaaaa ggacgttgat ttgcatcagg tctt
//
LOCUS       DADLZM010000353          926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLZM010000353 DADLZM010000000
VERSION     DADLZM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.360"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>926
                     /locus_tag="JNY73_004324"
     CDS             <1..>926
                     /locus_tag="JNY73_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HAY8050064.1"
                     /translation="DIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRML
                     FGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSENPIA
                     SKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTAF
                     KVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEI
                     YARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKT
                     KTGRLWVYVRDDRNAGSSLPAAVWFAYSADRK"
BASE COUNT          253 a          233 c          249 g          191 t
ORIGIN      
        1 tgatattgag ctgcttaaag ccatgttgcg taagcaacag agtcggcttc gacagtatgc
       61 ctgtcaggtc gcgggctatg agcaggaaat tgaacggctg aaagcgcaac tcgacaggtt
      121 gcgtcgtatg ttgttcggcc agagttcaga gaaaaagcgt cataagcttg aaaatcagat
      181 ccgacaggca gaaaaacgac tgtcggaact ggaaaaccgg ctgaacacag ccagaaatct
      241 tctggaagat gcatcgtcag tcacagattc acctgacacc agtcccccgt cagaaaaccc
      301 gatcgccagt aagcctgaat ccccgggacg aaaatcttca cgaaaaccgc tgccggcaga
      361 acttccccgg gagacacatc gccttctgcc tgctgaaacc agttgcccgg cctgtggagg
      421 tgttctgaaa gaaatggggg aaacaatctc agagcaactg gatatcatta ataccgcctt
      481 taaagttatc gaaaccatac gtcccaaact ggcctgtagc cggtgtgatg tcatcgttca
      541 ggcaccactt ccccctaaac cgatcgaacg cggttatgcc agtgcagggt tacttgcacg
      601 gatcctggtc agcaaatata tggaacatat ccctttatat cgccagtcag aaatatacgc
      661 gcgacagggc gtggagctga gccgtaatac catggtgcgc tgggtatcag aaatggcaga
      721 caaactccgt cctctgtata tagcgctgaa tgactatgtt ctggaggcag gaaaggtgca
      781 cgcagatgac actccggtga aagtactggc cccggggaac ggaaagacga aaacgggtcg
      841 tctgtgggta tacgtcaggg atgatcgtaa tgcgggttca tccctgccgg cagccgtctg
      901 gttcgcgtat tcggcagatc gcaaag
//
LOCUS       DADLZM010000354          908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLZM010000354 DADLZM010000000
VERSION     DADLZM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.361"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>908
                     /locus_tag="JNY73_004325"
     CDS             <1..>908
                     /locus_tag="JNY73_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8050065.1"
                     /translation="TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTL
                     WPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTC
                     GGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLV
                     LKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKT
                     GETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEP
                     VAKVCYAQMVKQFLSRDPFECVLCGGR"
BASE COUNT          207 a          226 c          298 g          177 t
ORIGIN      
        1 actaactcat gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg
       61 atagcgacac agctgaatcg tcttcctgac tgcgactggg tacacctggt cttcaccctg
      121 ccggacacgc tgtggccggt gttcgaaagc aaccggtggc tgctgaatga cgtgtgccgt
      181 ctggcggtgg agaatctgct gtatgccgcc cggaaacggg ggcaggaacc cggtatcttc
      241 tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc cgcatgtaca tgtgtctgta
      301 acctgtggag gtctgaataa gcatggtcag tggaaaaagc tgagcttcct gaaagacgcg
      361 atgcgttcgc ggtggatgtg gaatatgcgg caacggcttc tgaaagcgtg gtcagagggg
      421 ctggcgatgc cggagtcgtt gtcacatatc acgacggaat cacagaggag aagcctggtg
      481 ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga aaaaaacggc cggcgggagg
      541 aatacggcgc gctacctggg tcgttatctg aagaagccgc cgatagcggc ctcccggctg
      601 gcacattaca acggaggggc gagcctgagc ttccgttacc tggaccacaa aacgggagaa
      661 acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca gcacatcccg
      721 gagaagtttt ttaagatggt gaggtacttc gggttccttg ccagccgggt gtgtggagag
      781 aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt ggcgaaagtg
      841 tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg cgtgctgtgt
      901 ggcggccg
//
LOCUS       DADLZM010000355          894 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLZM010000355 DADLZM010000000
VERSION     DADLZM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.362"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(110..439)
                     /locus_tag="JNY73_004326"
     CDS             complement(110..439)
                     /locus_tag="JNY73_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY8050066.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..>894)
                     /locus_tag="JNY73_004327"
     CDS             complement(611..>894)
                     /locus_tag="JNY73_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050067.1"
                     /translation="FVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          217 a          207 c          199 g          271 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 ctatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaat
//
LOCUS       DADLZM010000356          881 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLZM010000356 DADLZM010000000
VERSION     DADLZM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.363"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..691
                     /locus_tag="JNY73_004328"
     CDS             <1..691
                     /locus_tag="JNY73_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8050068.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAM
                     TALLMKDNQRS"
     gene            793..>881
                     /locus_tag="JNY73_004329"
     CDS             793..>881
                     /locus_tag="JNY73_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8050069.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALR"
BASE COUNT          198 a          198 c          220 g          265 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctgttag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgttt ggtgccagtt atcgctacag tggtgctgga gtcggttatc aggttgccag
      541 tgtggttggc ggtggattta caccttttat tgccgctgca ctcatcactt actttgccgg
      601 gaactggcat agcatcgcca tttatttgct ggctggatgc ctgatttctg caatgaccgc
      661 tttattgatg aaagacaatc aacgctcgtg atagcctgcc gaagatcatc cgatcttcgc
      721 cttatacttt tgtttcacat tcctgtgaca tactatcgga tgtgcggtaa ttgtatggaa
      781 caggagacac acatgaataa taagggctcc ggtctgaccc cagctcaggc actggataaa
      841 ctcgacgcgc tgtatgagca atctgtagtt gctttacgca a
//
LOCUS       DADLZM010000357          863 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.364, whole genome shotgun sequence.
ACCESSION   DADLZM010000357 DADLZM010000000
VERSION     DADLZM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.364"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            169..>863
                     /locus_tag="JNY73_004330"
     CDS             169..>863
                     /locus_tag="JNY73_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY8050070.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLHELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGG"
BASE COUNT          228 a          221 c          235 g          179 t
ORIGIN      
        1 gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa ttaagggaca
       61 cagcctaaat agctgcgcgg aatagtagat ccctttgagg gaacttagcc cggattgtgc
      121 gatctgatca atcgccaaat caaaacaaat caccaaccgg actgagcaat gccgatcata
      181 gcaccaattt cccgtgacga acgacgcctg atgcagaaag ccatccataa aacacacgat
      241 aaaaattatg cccgcagact gactgccatg ctgatgctgc accggggcga ccgtgtcagc
      301 gacgttgcca gaacgctctg ctgcgcccgt tcctcggttg gacgctggat taactggttc
      361 acgcagtcgg gtgttgaggg actgaaatca ttacctgccg ggcgtgcccg tcgctggccg
      421 tttgagcata tctgcacact gttacatgag ctggtaaaac attctcccgg cgactttggc
      481 taccagcgtt cacgctggag tacagaactg ctggcaataa aaatcaatga gataaccggt
      541 tgccagttaa atgccggaac cgttcgccgc tggttgccgt ctgcggggat tgtgtggcga
      601 agggctgcgc caactctgcg tatccgtgac ccgcataaag atgaaaagat ggcagcaatc
      661 cataaagcac tggacgaatg cagcgcagag catccggtct tttatgaaga tgaagtggat
      721 atccatcttc atcccaaaat cggtgcggac tggcaactgc gcggacagca aaaacgggtg
      781 gtcacgccgg gacagaatga aaaatattat ctggccggag cgctgcacag cgggacaggt
      841 aaagtcagct atgtgggcgg caa
//
LOCUS       DADLZM010000358          853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLZM010000358 DADLZM010000000
VERSION     DADLZM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.365"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..761
                     /locus_tag="JNY73_004331"
                     /pseudo
     CDS             1..761
                     /locus_tag="JNY73_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          231 a          184 c          203 g          235 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agcgataacc cag
//
LOCUS       DADLZM010000359          830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLZM010000359 DADLZM010000000
VERSION     DADLZM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.366"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..326
                     /locus_tag="JNY73_004332"
     CDS             <1..326
                     /locus_tag="JNY73_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY8050071.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNY73_004333"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNY73_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY8050072.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          254 a          181 c          155 g          240 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
      481 atatcgacca ggcgaccttc tgcatgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gggtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagag cttctctaac atgtgttatt attaacaaag aaaaatgtaa
      781 gaagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga
//
LOCUS       DADLZM010000360          768 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLZM010000360 DADLZM010000000
VERSION     DADLZM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.367"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..496
                     /gene="ospC3"
                     /locus_tag="JNY73_004334"
     CDS             <1..496
                     /gene="ospC3"
                     /locus_tag="JNY73_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC3"
                     /protein_id="HAY8050073.1"
                     /translation="FVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKV
                     TDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLS
                     KDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGK
                     RFEI"
BASE COUNT          267 a          125 c          152 g          224 t
ORIGIN      
        1 ctttgtgttt cagcttgagt atcatatacc aagaatgcta agtacagata acttcaaaaa
       61 aataaaactc agagacatat ctttggagga tgcgattaaa gcatctaatt atgaagagat
      121 taacaataaa gtgactgata aaaaaatggc tcatcaggcc cttgcttatt ctttaggcaa
      181 taaaaaagct gatatagcac tttatttact atctaaattt aattttacaa aacaagatgt
      241 cgcagagatg gagaaaatga acaacaatag atattgtaat ctgtatgatg ttgaatactt
      301 acttagtaaa gatggtgcca actataaagt gctagaatat tttatcaata atggattggt
      361 tgatgtaaac aaaaaatttc aaaaagcaaa tagtggggac actatgcttg ataatgcaat
      421 gaaaagcaaa gattcaaaaa tgattgattt tttattaaaa aatggagcaa tattaggcaa
      481 acgatttgag atatgaccta aaagctcggc caaataataa tgtcgtcgat tatcgaagtc
      541 atcatgagtg attctgtccg cacagtgcag catcagcatg acagtgaatt gtctggcgtg
      601 gtccatttaa tgagatggag atggtccctc tttcctaaat agctgtgcct aataccgcta
      661 cacttttgcc agcccatgtt tgcctccggg gaatgggctg acggttcctt caaaatccat
      721 tgcacgcagt tatgtcggag cgggaacttg actcctgtct ttacggga
//
LOCUS       DADLZM010000361          756 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLZM010000361 DADLZM010000000
VERSION     DADLZM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.368"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="JNY73_004335"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="JNY73_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY8050074.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="JNY73_004336"
     CDS             103..375
                     /locus_tag="JNY73_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8050075.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            602..>756
                     /gene="ghoS"
                     /locus_tag="JNY73_004337"
     CDS             602..>756
                     /gene="ghoS"
                     /locus_tag="JNY73_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY8050076.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLN"
BASE COUNT          236 a          128 c          186 g          206 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggc
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 atcaatcggg gttaataagt tttgcgtccc cagagcgttt aatattgata ggagtcatat
      601 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
      661 ttcctcagtg ttcttaagat taagatcatt gatgtatgat atgaatttct cctctatcgt
      721 ggctgatgaa tatgggatac cacgacaatt gaatga
//
LOCUS       DADLZM010000362          752 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLZM010000362 DADLZM010000000
VERSION     DADLZM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.369"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..730)
                     /gene="ipaH9.8"
                     /locus_tag="JNY73_004338"
     CDS             complement(<1..730)
                     /gene="ipaH9.8"
                     /locus_tag="JNY73_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY8050077.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMS"
BASE COUNT          214 a          127 c          187 g          224 t
ORIGIN      
        1 cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt aatctttgca
       61 gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa cattcattcc
      121 tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa aaatactctc
      181 tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa ttacgggtcg
      241 ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg ttatagctga
      301 tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca ttgtgttgta
      361 cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta ttgctggcgg
      421 aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga ttatatgata
      481 cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga tttaaacggt
      541 ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag acagcctcat
      601 cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa aagtaatcag
      661 catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag ttattattta
      721 tcggtaacat gatgggcatc cagttaaagc cg
//
LOCUS       DADLZM010000363          737 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLZM010000363 DADLZM010000000
VERSION     DADLZM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.370"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            129..>737
                     /locus_tag="JNY73_004339"
     CDS             129..>737
                     /locus_tag="JNY73_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8050078.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVSHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPD"
BASE COUNT          155 a          200 c          219 g          163 t
ORIGIN      
        1 tatccggaaa tcgcccgctg cacggagcgg gaatttgcga ccattcttgc cggtatcagc
       61 gccggcggta tgtactgccc ggagtgtggc acggttcact ggccggacgg agtcgccccg
      121 cccttctgat gcttccccgt tttgccgata tttttcagca gggaaaccgc tggcttaact
      181 ggctggagaa acaaccggaa ggggcagtcc gtccggtggt aattgaatca gtgacaaaaa
      241 tcatggcctg cgggaccaca ctgatgggct acacgcagtg gtgttgttca tcaccggact
      301 gttgccatac aaaaaaggtc tgcttccggt gtaaaagccg ctcctgcccg cactgcgggg
      361 tgaaggctgg cgcacagtgg atacagtatc tgctgagcct ggtccccgac tgcccgtggc
      421 agcatattgt gttcacactt ccctgccagt actggtccct ggtgttccac aaccggtggt
      481 tactggcaga gatgagccgc attgcagcgg atgtgatact ggaaatctgc catcaggcag
      541 atgtggagcc ggggatattc acggtgagcc acacatgggg gcgtgaccag cagtggcatc
      601 cgcatatcca tttatcgaca actgccggtg gtgtgacgtc gggccacacc tggaaaaatc
      661 ttcattttta cgcccgtaag gtgatgagca tgtggcgcta ccggataaca cggttgctgt
      721 cacggaaata ccctgac
//
LOCUS       DADLZM010000364          736 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLZM010000364 DADLZM010000000
VERSION     DADLZM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.371"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(53..658)
                     /gene="capU"
                     /locus_tag="JNY73_004340"
                     /pseudo
     CDS             complement(53..658)
                     /gene="capU"
                     /locus_tag="JNY73_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
     gene            complement(661..>736)
                     /locus_tag="JNY73_004341"
     CDS             complement(661..>736)
                     /locus_tag="JNY73_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAY8050079.1"
                     /translation="LFYYTTPFFSSLLALHKGPRLPDD"
BASE COUNT          229 a          170 c          136 g          201 t
ORIGIN      
        1 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ctaaattcgt
       61 gccctttttc cgggcgcaac attcctacct gagcaattac ggggcatcct cttcgggacg
      121 ccagccagga aagaacactc ggaggcaaac tgtttcggga atcaacgtat aattcctgga
      181 aatcaaagcc aggaggcaca acagtaaccc gagttcgaca tccttcctgc tccagatgtg
      241 tcttcatatt tgttccggga acaatcacct catcgcaaaa atgatttatt gaaaaaaatt
      301 ttgtttttcg tgtcaaatat gtcttttgcc tgataattct gaatggatgt ttccaggtaa
      361 aaaaccgtac caaaccaaca atattactat catgtccact gtgacaaacg attgcatcgg
      421 gctgaaaact atgaactatt ccgagtaatc tccatacagt aggaatgtga agactgtttc
      481 gaaataacgc gaatgtgata tcaattccaa acttactggc ttcaaaagca attttgctgt
      541 tctccctgca gacaagcaga actgaatgcc ccattttctt cagggccttc atttgagcaa
      601 cagcctgtaa ttcctgacca cctatattcg gtgatgattc cgtaaatagt atattcatta
      661 ttagtcatcc ggtaatcttg gccccttatg aagggccagc aacgatgaaa aaaacggtgt
      721 tgtataataa aaaagt
//
LOCUS       DADLZM010000365          730 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLZM010000365 DADLZM010000000
VERSION     DADLZM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.372"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..200
                     /locus_tag="JNY73_004342"
     CDS             <1..200
                     /locus_tag="JNY73_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050080.1"
                     /translation="PTTGSTSPAVPFQITFSECSKGTTGVRVAFNGIEDAENNTLLKL
                     DEGSNTASGLGKKYWTEICVR"
     gene            188..355
                     /locus_tag="JNY73_004343"
     CDS             188..355
                     /locus_tag="JNY73_004343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8050081.1"
                     /translation="MRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLV
                     RASATFTLEFQ"
     gene            368..>730
                     /locus_tag="JNY73_004344"
     CDS             368..>730
                     /locus_tag="JNY73_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8050082.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKN"
BASE COUNT          210 a          161 c          176 g          183 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgttt agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat
//
LOCUS       DADLZM010000366          729 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLZM010000366 DADLZM010000000
VERSION     DADLZM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.373"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>729
                     /locus_tag="JNY73_004345"
     CDS             <1..>729
                     /locus_tag="JNY73_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HAY8050083.1"
                     /translation="LRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALL
                     RRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDG
                     FEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSS
                     PVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMP
                     KPDGLTAAKNLAEAFEHYNEWHPH"
BASE COUNT          180 a          177 c          222 g          150 t
ORIGIN      
        1 ttctcagacg aaccgatgac tggatggatg gccgccgcag tcgtcacact gatgatacgg
       61 atgtgcttct ccgtatacac catgttatcg gagagctgcc cacgtatggt tatcgtcggg
      121 tatgggcgct gcttcgcaga caggcagaac ttgatggtat gcctgcgatc aatgccaaac
      181 gtgtttaccg gatcatgcgc cagaatgcgc tgttgcttga gcgaaaacct gctgtaccgc
      241 catcgaaacg ggcacataca ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct
      301 ctgacgggtt cgagttctgc tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg
      361 actgctgtga tcgtgaggca ctgcactggg cggtgactac cggcggcttc aacagtgaaa
      421 cagtacagga cgtcatgctg ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt
      481 ctccagtgga gtggctgacg gataatggtt catgctaccg ggctaatgaa acacgccagt
      541 tcgcccggat gttgggactt gaaccgaaga acacggcggt gcggagtccg gagagtaacg
      601 gaatagcaga gagcttcgtg aaaacgataa agcgtgacta catcagtatc atgcccaaac
      661 cagacgggtt aacggcagca aagaaccttg cagaggcgtt cgagcattat aacgaatggc
      721 atccgcata
//
LOCUS       DADLZM010000367          699 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLZM010000367 DADLZM010000000
VERSION     DADLZM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.374"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..311)
                     /locus_tag="JNY73_004346"
     CDS             complement(<1..311)
                     /locus_tag="JNY73_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8050084.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     M"
     gene            complement(375..>699)
                     /locus_tag="JNY73_004347"
     CDS             complement(375..>699)
                     /locus_tag="JNY73_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY8050085.1"
                     /translation="RNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTD
                     NKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVG
                     VGYKF"
BASE COUNT          165 a          233 c          155 g          146 t
ORIGIN      
        1 ttcatcacct ccggacgtaa atcaccatcc tttggtgcat tcagaaacgc gttcagtgtc
       61 cccggtgcat cagtcggtgc cacatcaata tccccgacac gggttggctg ccagccgtta
      121 cagtgaagcg caacctcgta ataccccggc tcagcctcaa tcacataagc accgttattg
      181 tccgtcacgc aagtggcaac aacgtgtgcc acaacggtcg gactggttct tctggcccgc
      241 agttcaatcg cacaatttac gacaggttta cccgccccat ctttcagtac acctgaaatc
      301 tttactgcca tattcacccc acaaaaagcc cgcctgaacc agcgggctgt cataacactg
      361 tgttacctgg ctaatcagaa cttataaccg acacccacaa tgaaaccgtc agtgcgccag
      421 tcgccactgc cggaaccttc ataagcaagg tcaatggcca cggattcggt cgggttaaac
      481 tgcacgccag ccccccacgc cagagacgtg ttgctgtggc ggttgtcatc actcccggtc
      541 agcacatcgt gcgttttccc cttgttatca gttacgcgga gataatcccc ggagaaggtc
      601 gacacacggc tgtaagccac gcccgccatc gcatacgcgc tgaaccattc attcacgcgc
      661 acagacggcc ccgccatcac gctgaaccag cggttacgc
//
LOCUS       DADLZM010000368          664 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.375, whole genome shotgun sequence.
ACCESSION   DADLZM010000368 DADLZM010000000
VERSION     DADLZM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.375"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>664
                     /gene="ipaH3"
                     /locus_tag="JNY73_004348"
     CDS             <1..>664
                     /gene="ipaH3"
                     /locus_tag="JNY73_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY8050086.1"
                     /translation="SAWDKWEKQALPGENRNEAVSLLKECLINQFSELQLNRLNLSSL
                     PDNLPPQITVLEITQNALISLPELPASLEYLDACDNHLSTLPELPASLKHLDVDNNQL
                     TMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTN
                     LLESLPAVPVRNHHSEETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRE
                     SLS"
BASE COUNT          198 a          177 c          117 g          172 t
ORIGIN      
        1 tttcagcatg ggataaatgg gaaaaacaag cgctccccgg tgaaaatcgg aatgaagcgg
       61 tctccctact taaagaatgt ctcatcaatc agttcagtga gcttcaactg aatcgtttaa
      121 atctgtcctc gctacctgac aacttaccac ctcaaatcac tgttctggaa attactcaga
      181 atgccctaat atcattacca gaattgccag catcgctgga ataccttgac gcctgtgaca
      241 atcacctgtc aacacttcct gaattacccg catctctgaa acatcttgat gtagataaca
      301 accaactaac catgcttcct gaattgcctg cattgctgga atatattaat gcagataaca
      361 atcagctaac catgcttcct gaattaccta catcgctgga agtgctctca gtaagaaata
      421 accagctgac atttctccct gagttacctg aatcactgga agcgctcgat gtaagtacta
      481 atcttctgga aagcctacca gccgtacctg taagaaatca tcactcagag gaaaccgaga
      541 tatttttccg gtgccgcgag aatcgcatca cacacattcc ggaaaatata cttagccttg
      601 atccgacctg cactatcatc ctcgaagaca atcctctgtc ctcacggatc agggagtctc
      661 tgtc
//
LOCUS       DADLZM010000369          658 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLZM010000369 DADLZM010000000
VERSION     DADLZM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.376"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(151..657)
                     /locus_tag="JNY73_004349"
     CDS             complement(151..657)
                     /locus_tag="JNY73_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY8050087.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          173 a          160 c          146 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttggcgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaatc
//
LOCUS       DADLZM010000370          657 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLZM010000370 DADLZM010000000
VERSION     DADLZM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.377"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            101..>657
                     /locus_tag="JNY73_004350"
     CDS             101..>657
                     /locus_tag="JNY73_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8050088.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          108 g          192 t
ORIGIN      
        1 caatacctgg agaaagagta caaagcaaac tgtattaaac ttaataagtt atattgtatt
       61 tcactagagt tcttatcgtt gataagaaat tctggttata atgattaaat caacaaatat
      121 acaggtaatc ggttctggta ttatgcatca aataaacaac atacactcgt taactctatt
      181 ttctttgcct gtgagtttga gtccatcttg taatgaatat tatttaaaag tctggagtga
      241 atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata ggctaaaaat
      301 atgtttgcaa aatcaagggg cggaattaaa tctatctgag ttagatttaa aaacattacc
      361 tgatttaccg cctcagataa caacactgga aataagaaaa aacctattaa catatctccc
      421 tgatttccca ccaatgctta aggtaataca tgctcaattt aatcaactgg aaagcttacc
      481 tgccttaccc gagacgttag aagagcttaa tgtgggtgat aacaagataa aagaattacc
      541 atatcttcct gaaactctaa ctcatttacg gatccataat aaccgattgc atattctgcc
      601 actattgcca ccggagctaa aattactgat agtttctgga aacagattag acagcat
//
LOCUS       DADLZM010000371          644 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLZM010000371 DADLZM010000000
VERSION     DADLZM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.378"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(15..230)
                     /locus_tag="JNY73_004351"
     CDS             complement(15..230)
                     /locus_tag="JNY73_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8050089.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
     gene            complement(523..597)
                     /locus_tag="JNY73_004352"
     tRNA            complement(523..597)
                     /locus_tag="JNY73_004352"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(562..564),aa:Gly,seq:tcc)
BASE COUNT          200 a          158 c          141 g          145 t
ORIGIN      
        1 taatgatggc gacattattc acctctcgca gccttacgct tgtcttctct gattttgaag
       61 tacagatttg tcagataagt caggaagccc agaaccagac tccccagtac accaatcgca
      121 gcccactgtg acggactgac ctgatcaagc cactgtaaaa accagtagcc agcactgcct
      181 gcggaggtgc cgtaggcaat gcccgttgaa attttgtcca tggatttcat agcctcacct
      241 ccgcaaataa cggatggcgt agttttacac tgagaaatga aagggatttg aaaagaaaaa
      301 accgcaaaag cgggcgaaac gatatataca gtaaggaaag cactctatcc aacaaaccac
      361 tcacagttaa tcggaataaa agcagagtgc ttatgaatga tcgcctgccc gaaggttagt
      421 atttctgcac agcaattttg caaaaaaaag cgatcattca taacttaaac gtctttcagt
      481 cactccggga tttcccatca tcgcagactg aaagactcta actggagcgg gcagcgggaa
      541 tcgaacccgc atcatcagct tggaaggctg aggtaatagc cattatacga tgcccgcata
      601 tggtgccgac taccggaatc gaactggtga cctactgatt acaa
//
LOCUS       DADLZM010000372          636 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLZM010000372 DADLZM010000000
VERSION     DADLZM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.379"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            88..153
                     /locus_tag="JNY73_004353"
                     /pseudo
     CDS             88..153
                     /locus_tag="JNY73_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JNY73_004354"
     CDS             381..629
                     /locus_tag="JNY73_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY8050090.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DADLZM010000373          626 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLZM010000373 DADLZM010000000
VERSION     DADLZM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.380"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..173)
                     /locus_tag="JNY73_004355"
     CDS             complement(<1..173)
                     /locus_tag="JNY73_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY8050091.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVS"
     gene            complement(170..532)
                     /locus_tag="JNY73_004356"
     CDS             complement(170..532)
                     /locus_tag="JNY73_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY8050092.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          201 a          139 c          119 g          167 t
ORIGIN      
        1 tcagaaacag ccagagcgtt aatgattgac tccgtagcgt ctttgcttcc gtcatttatg
       61 aaaacaattt ctacttcata tggcttcaac tcttcgaatt cacgtaccgt tttataaaaa
      121 attggtatcg cttcttcttc attgaagaca ggaacgacaa gagatatctt catttcgcat
      181 ccctaaagac aatgaacttt gaatagacga aaccgcacac caagctgatg gcggagaagg
      241 taacaagagt gacaattgga ggaagtgaac atctatcagc aacccatcca acagtagcac
      301 tcagtgttcc catgaacccg acatataaca tgtagcgcat cgttgtagtt gatgctttga
      361 atgtgaattt tgcattcgca aagaagctaa aactcacagc cacaacgaaa cctgcgaagt
      421 ttgccatagc ctgattggta tgcgcggcat agatacatac accaaaaacc acccagtgta
      481 taagtgtgtt cagcacgcct atagatgtgt actttgcaaa tagctttaac atttcttcta
      541 tcagctaata atcaaaggca tgaagtctat catccaagtc tcaatcgatc gatacttgcg
      601 gtagttgatg agaaaaactc tggcac
//
LOCUS       DADLZM010000374          623 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLZM010000374 DADLZM010000000
VERSION     DADLZM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.381"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>623
                     /locus_tag="JNY73_004357"
     CDS             <1..>623
                     /locus_tag="JNY73_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8050093.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTTGGFNSETVQDIMLGAVERRFGNELPASPVEWLTDN
                     GSCYRANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYIS"
BASE COUNT          149 a          152 c          195 g          127 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actacgggcg gcttcaacag tgaaacagta caggacatca tgctgggagc
      421 ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca gtagagtggc tgacggataa
      481 tggttcatgc taccgggcta atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc
      541 gaagagcacg gcggtgcgga gtccggagag taacggcata gcagagagct tcgtgaaaac
      601 gataaagcgt gactacatca gtg
//
LOCUS       DADLZM010000375          622 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLZM010000375 DADLZM010000000
VERSION     DADLZM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.382"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(66..289)
                     /locus_tag="JNY73_004358"
                     /pseudo
     CDS             complement(66..289)
                     /locus_tag="JNY73_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          179 a          120 c          156 g          167 t
ORIGIN      
        1 acattatagt cggctaaatg aagtttactg taaaaaaacg atactttttc gggtggcgct
       61 tccaatcagt agaatatgtg tttttcaatg aaggagccga tgacttttcg tggatttcca
      121 cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcgggtg cggagggtca
      181 ggttgttacg ttcaagatat agcatgtgct ggatgaagat cccttttctg gtcacctgtt
      241 tatctttcgt ggccgacggg gtgacactgt taaaatgtaa tgaacgcatc ccacacaatt
      301 aaacacggag tagtatgatg gttaaaaggg tgaaactggc aggtgtggag cctcgcagct
      361 ttccggcatc atactgccca atacgggatg cataaagcgt cctcagactg gagactccac
      421 aaatgaaata tacaccggtt ggcgttgata tggcaaaaca tgtcattcag attcacttca
      481 tcaatgagca catgtcagcg ccagtgatat aagacggtaa tggggatgcg gacgatttcc
      541 tggactctca gaagatgaaa catgtttact gaagaacaaa aaattcgcgc tattgagctt
      601 tatttcaaat atggtaagaa gc
//
LOCUS       DADLZM010000376          615 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLZM010000376 DADLZM010000000
VERSION     DADLZM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.383"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="JNY73_004359"
     CDS             complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="JNY73_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY8050094.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSYFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          187 a          122 c           91 g          215 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaatta tatacaaaaa ataactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgtt
//
LOCUS       DADLZM010000377          614 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLZM010000377 DADLZM010000000
VERSION     DADLZM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.384"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..183
                     /locus_tag="JNY73_004360"
     CDS             <1..183
                     /locus_tag="JNY73_004360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050095.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            278..469
                     /locus_tag="JNY73_004361"
     CDS             278..469
                     /locus_tag="JNY73_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY8050096.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKE"
     gene            548..>614
                     /locus_tag="JNY73_004362"
     CDS             548..>614
                     /locus_tag="JNY73_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8050097.1"
                     /translation="MNSQTTKDIPCFRSYLPDALHL"
BASE COUNT          178 a          132 c          165 g          139 t
ORIGIN      
        1 caactcgaaa aaacgggcat aacaaaagat gctatccgta aagatatggt acccgcgctt
       61 cgggaactga tgcaggcgaa gcatcgcgca cagaaagcag tagatgagct ggtggactgt
      121 gtggcagagc tggaaaccag agtcggaaag tttgaaaaac tggtgcagga ggcgctgcgc
      181 tgatgcgcca tgagtttgtt ttgccctggc ttgtgcgcga tcattatgtc cctggtggaa
      241 agcccgggat aaaaatcacc gaaccggaga gtatttgatg agcgtcaaaa ttcaaacgat
      301 accggaactg cttatccaga cacgaggtaa tatgaccgaa gtttcacgaa tgctgaactg
      361 taatcgcgcc acggtaagaa aatatgctga agataaagag ggcaaagggc acgccattgt
      421 tgatggtgtt ctgattgttc atcgtggatg ggacaggggt aaagagtaag cgccccatca
      481 gcgacgtctt gtgaaaattg tcctgtctgg caacaatcgc gcccatctat attgatggac
      541 acgaacgatg aattcccaga caacaaaaga tattccctgc ttccgttctt atttgcctga
      601 tgccctgcat ttaa
//
LOCUS       DADLZM010000378          612 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLZM010000378 DADLZM010000000
VERSION     DADLZM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.385"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT          221 a          108 c          128 g          155 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa at
//
LOCUS       DADLZM010000379          599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLZM010000379 DADLZM010000000
VERSION     DADLZM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.386"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>599)
                     /locus_tag="JNY73_004363"
     CDS             complement(<1..>599)
                     /locus_tag="JNY73_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8050098.1"
                     /translation="AALTGTLKTANHLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSD
                     IYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIP
                     DTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCYWPISRLTGWVKIN
                     ITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGD"
BASE COUNT          129 a          163 c          153 g          154 t
ORIGIN      
        1 ttatcaccgt cagcttctcc ctggtacgca gcaataatca gcccgccaat gtcagggtct
       61 ccccatctgt tgcggacaga gctcgccacg attctgtaaa taatatcttc tgtggttata
      121 tttattttca cccagccagt cagtctggat atgggccagt agcagtagcg ggtatgataa
      181 atggggccgt taatgccgta aaaagtaagg gatttggctc tgtaccgcgg ttctgttgtc
      241 tcagggcgtg catcagtcca ccggatgctg agcacccctt caaaccgtgt gtcgggtatg
      301 atgatgccgt aagggccagc aacggaatat tcacctggca gcgcattcct tacccaggcc
      361 aggaaatcac tcttagtgtc aaaacggata acatcttcag gcagaaaagc acacccaaag
      421 ccgaatgcgc cgggtatcgc cagacggcct tttgtccggt cgtaaatgtc gctctgtgct
      481 tccatcgtgg ccgcactttt cagccccaga ttatcccggg acttctgttg tgccttttcg
      541 cctgctgctg cgatttcaga cagatggtta gccgttttca gggtgccggt cagcgcagc
//
LOCUS       DADLZM010000380          596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLZM010000380 DADLZM010000000
VERSION     DADLZM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.387"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..323
                     /locus_tag="JNY73_004364"
     CDS             <1..323
                     /locus_tag="JNY73_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8050099.1"
                     /translation="ARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDT
                     PEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMK
                     GGIR"
     gene            320..>596
                     /gene="tnpB"
                     /locus_tag="JNY73_004365"
     CDS             320..>596
                     /gene="tnpB"
                     /locus_tag="JNY73_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8050100.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQ"
BASE COUNT          145 a          157 c          172 g          122 t
ORIGIN      
        1 ttgcccggat cgccagagaa cacgatatca ataataacct gctgtttaaa tggaaaagcc
       61 agtacgagga cggcttactg agcgatgatg acatacagga atgcatgcct gtcccggtgg
      121 cactgactga tacgccggag ccgaccagac cagttacaaa tcccttctgg cgtaacaagc
      181 atgatgagcg ccctgagggg gctcccggaa acgtcccacg gtgcgagctg catcttaaat
      241 caggtgtggt aaaactgttt gaccctctca ctccggaact gttacgggcg ctaatccgcg
      301 aaatgaaagg gggtatccga tgataacgct gccgaccggt accaaaatct ggatcatcgc
      361 tggcatcaca gatatgcgtt gtggcttcaa tggcctggct tcgaaggtgc agaacacgct
      421 gaaagatgac ccgttctccg ggcatatctt cgtcttccgg ggccgcagtg gcaaaatggt
      481 gaaaatactg tgggccgatc gtgacgggtt atgcctgttc gccaaacgcc tggaacgggg
      541 ccgcttcgtc tggccggtga cccgggaagg gaaagtgcac ctgacgccag ctcagt
//
LOCUS       DADLZM010000381          595 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLZM010000381 DADLZM010000000
VERSION     DADLZM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.388"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            58..555
                     /locus_tag="JNY73_004366"
                     /pseudo
     CDS             58..555
                     /locus_tag="JNY73_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          144 a          147 c          183 g          121 t
ORIGIN      
        1 cgccagcccc gtcccgcccg atagagcgcg gcatagcggg ccccggatta cttgcgtgcg
       61 acggcagaag aagccctgaa gcgaatcagt gaactgtacg ccatcgagga tgaaatacgg
      121 ggattaccag agtcagagtg tcttgcagtc aggcagcagc gaagcaaagc gttactgacg
      181 tcgctgcatg aatggatggt ggagaagaat ggcacgctgt cgaaaaaatc cagactgggc
      241 gaagcattca gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt
      301 ctggcggagg cggacaataa cacagcggaa agagcgcttc gtgcagtctg tctcggaaag
      361 aaaaattacg tgttcttcgg tagcgatcac ggcggcgagc gtggtgcact gctgtacggg
      421 ctgatcggca cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg
      481 agcgtactgc cggaatggcc ttccaaccga gttgacgacc tcctgccatg gaaggtggtg
      541 ctaccatcag gataatcgtc aatacggtgt tcggtgaacg tttacttgga gcatc
//
LOCUS       DADLZM010000382          577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLZM010000382 DADLZM010000000
VERSION     DADLZM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.389"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            95..>577
                     /locus_tag="JNY73_004367"
     CDS             95..>577
                     /locus_tag="JNY73_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY8050101.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQGNAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     L"
BASE COUNT          156 a          119 c          171 g          131 t
ORIGIN      
        1 tcgacgacga gcagtttgat gaaaccaata ttgtgcgcgg actgcctgtt cctggtggtc
       61 ggttggggat aaaaatcaca gagctggagt gtgcatgaat aaccagtatt tacagtttgt
      121 tcgtgagcag ctcattatcg ccaccgctga tttgagtggg gcgacaaaag gtcagcttga
      181 agcctggcag gggaatgcca tgttcgatac agggcgttac aggcgtaaaa aaatccggta
      241 ccgcgatgag gtgaccggaa aaatgataac gcgggataat ccaccaatcc cgggaaaaca
      301 atcactggcg aagggggcgt caattcctct ggtcagtcag gttgcgtttt cgacatcatc
      361 gtggcggcgg gctgttctgt ctcttgaaga gcaccataaa gcctggttgt tgtggtgtta
      421 cagcggaagt atttgctggg agtatcagat caccataacc cactgggcgt gggaagagtt
      481 taaggctcat tctggcacta ggaaaattgc agagaaaaca caggaacgcc tgaaaaaatt
      541 aatctggctg gcggcgcagg cggtaaaagc agaactt
//
LOCUS       DADLZM010000383          574 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLZM010000383 DADLZM010000000
VERSION     DADLZM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.390"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..276
                     /locus_tag="JNY73_004368"
     CDS             <1..276
                     /locus_tag="JNY73_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8050102.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGFDNGGESAA
                     IIYSLLFTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          140 a          128 c          151 g          155 t
ORIGIN      
        1 ctgaacgtgt actgtcgtaa tggccaggtg gaaatcgaca acaacatcgg tgaaaacgcg
       61 ctgcgatcgg tggcggttgg aagaaaaaat tatctcttct tcggttttga caacggtgga
      121 gaaagcgcgg cgattatcta cagtctgctg ttcacctgca aactgaacgg agtggagccg
      181 gaggactggt tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgagtgcat
      241 gaactgctgc cctggaactt ctcagctgta aaataatcct tccgctacgt acttctctgg
      301 gcgcttacca ctcaacggca tcgtcaaaat tatcaaagcg cagaacatag atttatcatg
      361 tgttttatgg atggctttct ccatcaggca tcgttcgtca cgggaaattg gtgctatgat
      421 cggcattgat cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
      481 tccgggctga gttccctcta agtgatctag gttgtgtccc ttaattacgt aagcgttgat
      541 aaaagagtcg gatgcagccc agtttcacca tcgc
//
LOCUS       DADLZM010000384          572 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLZM010000384 DADLZM010000000
VERSION     DADLZM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.391"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..261
                     /locus_tag="JNY73_004369"
     rRNA            <1..261
                     /locus_tag="JNY73_004369"
                     /product="16S ribosomal RNA"
     gene            318..394
                     /locus_tag="JNY73_004370"
     tRNA            318..394
                     /locus_tag="JNY73_004370"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:352..354,aa:Ile,seq:gat)
     gene            437..512
                     /locus_tag="JNY73_004371"
     tRNA            437..512
                     /locus_tag="JNY73_004371"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:470..472,aa:Ala,seq:tgc)
BASE COUNT          145 a          130 c          156 g          141 t
ORIGIN      
        1 gtcgtagtcc ggattggagt ctgcaactcg actccatgaa gtcggaatcg ctagtaatcg
       61 tggatcagaa tgccacggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca
      121 tgggagtggg ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct taccactttg
      181 tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg cggttggatc
      241 acctccttac cttaaagaag cgttctttgt agtgctcaca cagattgtct gatgaaaatg
      301 agcagtaaaa cctctacagg cttgtagctc aggtggttag agcgcacccc tgataagggt
      361 gaggtcggtg gttcaagtcc actcaggcct accaaatttg cacggcaaat ttgaagaggt
      421 tttaactaca tgttatgggg ctatagctca gctgggagag cgcctgcttt gcacgcagga
      481 ggtctgcggt tcgatcccgc atagctccac catctctgta gtgattaaat aaaaaatact
      541 tcagagtgta cctgcaaagg ttcactgcga ag
//
LOCUS       DADLZM010000385          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLZM010000385 DADLZM010000000
VERSION     DADLZM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.392"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..297
                     /locus_tag="JNY73_004372"
     CDS             <1..297
                     /locus_tag="JNY73_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY8050103.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JNY73_004373"
     CDS             complement(405..>566)
                     /locus_tag="JNY73_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050104.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADLZM010000386          556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLZM010000386 DADLZM010000000
VERSION     DADLZM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.393"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            122..>556
                     /locus_tag="JNY73_004374"
     CDS             122..>556
                     /locus_tag="JNY73_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8050105.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDVATNVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          159 a          106 c          127 g          164 t
ORIGIN      
        1 aagatttgtc acttattgga tttagtatgc ttgatttaaa tggcggtcaa ttattgttat
       61 tattaagtct aatgtcattt aaaggcatta tcatatttac gaagagatca ggatgatgac
      121 gatgaaaaaa agtgtattga cggcgtttat aactgtggta tgtgcaacgt ccagcgttat
      181 ggctgctgat gataatgcta tcacggatgg ctcagtaaca tttaatggta aagttattgc
      241 tccagcttgt accctggtag ctgcgacgaa agattcagta gtcacattgc caaatgtcag
      301 cgccaccaag ttgcaaacta atggagcagt ctctggcgtt aaaactgatg taccaattgc
      361 cttagaaggc tgtgatgtta ctgtaactaa aaatgcgacg tttaccttta gtggtacggc
      421 tgacggcgtg caacctacag catttgccaa ccaggctacc accgatgtag cgacgaatgt
      481 tgctctacaa atgtatctgc cagatggttc tacatcggta actcccggta ccgaaacgtc
      541 gaatattcag ttagcg
//
LOCUS       DADLZM010000387          556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLZM010000387 DADLZM010000000
VERSION     DADLZM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.394"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(206..>556)
                     /locus_tag="JNY73_004375"
     CDS             complement(206..>556)
                     /locus_tag="JNY73_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY8050106.1"
                     /translation="REARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGSLSPVVM
                     LAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELS
                     GLDDLASIRAFDVK"
BASE COUNT          115 a          187 c          121 g          133 t
ORIGIN      
        1 acggctcaca actgcaccat tcataatcac atcgcataaa ccacagaccg gactgactcc
       61 ggaaaaacag aggcccgccc ccgggcctcc ccggattcat ccgtttctct gttcagcctg
      121 accgcacacc ccggcggccg gatgacagac tccgcctcgg taagcaaagc ggtcttctgt
      181 gattccgcca gttgcggctt attcattact taacgtcaaa cgcccgaatt gaagccaaat
      241 catccagacc gctcagctcc tctttcattt cacgctgacg gcgataaatc tcatcgttgc
      301 gatcgacctg cgcctgcacc attgctgccg ccagttcttc cagttccggc atcgacagtt
      361 tcacctgctg attatccgca tcgctccacg ccatatgtgt ttgtgctgtg acagattttg
      421 ccagcatgac taccggggac aggctgccca gtgagtcggg accagcattc cagatacgac
      481 cgttccattc aaacgtgaac ggcttcgcct cctgttctgt gcgccatgct tcaatttcct
      541 gacgtctggc ctctct
//
LOCUS       DADLZM010000388          553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLZM010000388 DADLZM010000000
VERSION     DADLZM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.395"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(28..>553)
                     /locus_tag="JNY73_004376"
     CDS             complement(28..>553)
                     /locus_tag="JNY73_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8050107.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGAAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT          144 a          139 c          134 g          136 t
ORIGIN      
        1 accagtattt cttcggagtg aagaatatta ccaagtatat ttaacaccca cgttcgcgga
       61 ccagtcttga tctacgtcac caccaccgag gtagttagca tcggtatagg cgctgaagtt
      121 cttggtgaag ctgaactgag tacccagacc aacacgtacc gcagcccctt cagtaccgtt
      181 atcgatggaa tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag
      241 tttgaagtac ggagtcagag cctgatcttc gctgtaggtg aaggtataac ctgcatctac
      301 acccagttca taacgcatgc tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag
      361 ctggtagtca tcaccagact ggaacagacc agaaatgctg ccgtaaggag tcacgtaacc
      421 agcatcaccc agtttgaagt cgtaaccggc tttcaaaccg aagccccaag cgtcggagtt
      481 ggtgctaccg tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg
      541 agagtagctc aag
//
LOCUS       DADLZM010000389          545 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLZM010000389 DADLZM010000000
VERSION     DADLZM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.396"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            6..>545
                     /locus_tag="JNY73_004377"
     CDS             6..>545
                     /locus_tag="JNY73_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY8050108.1"
                     /translation="MKWLAENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWF
                     QVYPLLNEFNLKAHIFLITSFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSE
                     VNEMLQSGLVEFHVHTHTHTRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHL
                     CWPEGYYNKDYIQIAEELGF"
BASE COUNT          175 a           90 c          119 g          161 t
ORIGIN      
        1 aacaaatgaa atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt
       61 ttttttatcg gggcggaaaa cttccccgta aaagcgttat gttgacattt gatgatggat
      121 accttgacaa ctggtttcag gtatatcctt tattaaacga gtttaatctc aaggcacata
      181 tttttttaat taccagtttt attggaaatg gtccggttcg tcactctcct ggaaaggaat
      241 attctcatcg cgattgcgaa catcaaatag caaccgggaa tgctgataat gttatgcttc
      301 gctggtctga agtgaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacac
      361 atactcatac acgttgggat aaaaagttca cttcacgaga agaacagtgt aaacatcttc
      421 ggcaggactt attgagcggg agagaatatt taaaaaaaat gacaggaaaa tgcagtaaac
      481 atttgtgttg gccggaaggt tattataaca aggattatat tcagatcgct gaagaacttg
      541 gattc
//
LOCUS       DADLZM010000390          539 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLZM010000390 DADLZM010000000
VERSION     DADLZM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.397"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            56..>539
                     /locus_tag="JNY73_004378"
     CDS             56..>539
                     /locus_tag="JNY73_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8050109.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYRRMLYIEFTDNMR
                     Y"
BASE COUNT          134 a          137 c          137 g          131 t
ORIGIN      
        1 caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag agcttatggt
       61 cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga gtagccgggc
      121 gattgccaga gaactggggc tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc
      181 tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg aataccggga
      241 ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa cggtaatcgc
      301 tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg cattcattcg
      361 ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg aacccggacg
      421 acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt
      481 tgctgttccc ggatacagac gaatgttgta catcgaattc actgacaata tgcgttatg
//
LOCUS       DADLZM010000391          531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLZM010000391 DADLZM010000000
VERSION     DADLZM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.398"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>531)
                     /locus_tag="JNY73_004379"
     CDS             complement(<1..>531)
                     /locus_tag="JNY73_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8050110.1"
                     /translation="DIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDF"
BASE COUNT          129 a          156 c          143 g          103 t
ORIGIN      
        1 aaagtccatt tcgaagccgc gacgacacat aatgcgcatc accacgatgt cggtggcttc
       61 accgccgaga gtgaaggccg gaacctgcca gccgcgcaga cgcagacgtt cagagaggtc
      121 gtacagggtg tatcccggat cttcaccatc tttcagtttg aagcaaaccg ccgggatgcc
      181 ttcgtccggg cgacccgtac agatgaactc atacggcccc tgtttggcga tttcatccgc
      241 cagataagcg gcaacctggt aagaggcgtt ctgtactttg gtatagcctt cacgaccgag
      301 gcgcaggaat tcatagtact gtgcaattac ctgacccgcc gggcgggaga agttgatggc
      361 aaaagtacca atttgaccac ccaggtagtc aacgttgaac accagttcct gcggcagcgc
      421 ttcttcgtca cgccagataa cccagccgca gcccagcgga gccagaccga atttatggcc
      481 tgaagcactg atcgatttca cacgcggcag gcggaagtcc cagacgatat c
//
LOCUS       DADLZM010000392          525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLZM010000392 DADLZM010000000
VERSION     DADLZM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.399"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>525)
                     /locus_tag="JNY73_004380"
     CDS             complement(<1..>525)
                     /locus_tag="JNY73_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8050111.1"
                     /translation="CADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVV
                     DVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTI
                     TVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDVA
                     LAVVAHPNADNAMQ"
BASE COUNT          149 a          124 c          105 g          147 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaat
//
LOCUS       DADLZM010000393          522 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLZM010000393 DADLZM010000000
VERSION     DADLZM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.400"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            453..>522
                     /locus_tag="JNY73_004381"
     CDS             453..>522
                     /locus_tag="JNY73_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8050112.1"
                     /translation="MEPVYVILNALLDSGRFTRKLIL"
BASE COUNT          119 a          128 c          143 g          132 t
ORIGIN      
        1 tacccgatta gttgttaatc atatttaatc atagtgatta ctttcagacg gaacaaggca
       61 aataatgcga gtcagcgcat ataaggcggc atacggtggt tacatgtagc acaggtgtaa
      121 aaccgccgaa gcccggcgca agccggtacg gtcgtccgaa tatcaccagt ccccctccgg
      181 ctctgcgagg ccatgcggcc tgtcagcgat gaaaatgcct ctgtgcgcgg tcggagtggt
      241 cccgacaagg gtttacccgc agtcggggcg tatctccgcg ttagcgggcc gtgagggccg
      301 cttacgagcg tgtatgagag acgtacagta gttttgtccg ctcacaccag tacaaaccag
      361 tcgaaatacc ggtaacgacg attactcttg tctctgatgc cggtggacta cgagatttta
      421 tttactggcg tcctgatgcg gtcaggactg gtatggaacc tgtttatgtg atacttaatg
      481 ctcttctgga ttccgggcga tttacgcgta aacttattct gt
//
LOCUS       DADLZM010000394          517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.401, whole genome shotgun sequence.
ACCESSION   DADLZM010000394 DADLZM010000000
VERSION     DADLZM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.401"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>517
                     /locus_tag="JNY73_004382"
     rRNA            <1..>517
                     /locus_tag="JNY73_004382"
                     /product="23S ribosomal RNA"
BASE COUNT          154 a          114 c          157 g           92 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccg
//
LOCUS       DADLZM010000395          512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.402, whole genome shotgun sequence.
ACCESSION   DADLZM010000395 DADLZM010000000
VERSION     DADLZM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.402"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>512)
                     /locus_tag="JNY73_004383"
     CDS             complement(<1..>512)
                     /locus_tag="JNY73_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8050113.1"
                     /translation="REGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVL
                     QRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENML
                     DQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFG
                     TLKSECFYLD"
BASE COUNT          133 a          120 c          102 g          157 t
ORIGIN      
        1 atcaagataa aaacactccg actttaaggt tccaaagaaa cactccacca cagcattatc
       61 cagacaattg ccttttctgg acatgctttg tttaatacca tgttctttaa ggatattttg
      121 atatcttctc atacgatact gccatccctg gtcagagtgc agaacaggat gctcgtgagg
      181 attaagcttt ttgaatgcct gatcgagcat attctcaacc atgttcatca ctggtctttc
      241 cgaaaggctg taagaaataa cttcgttgtt gaagagatct attactggag acaaatacag
      301 cttgcgccca ttgactgcaa attcagtaac atcggtaacc cacttctcgt ttggccgcgt
      361 agccttgaaa tctctttgga gaacattagg ggcggtttgc cctacctctc ctctgtaaga
      421 gcggtatcgc ttgaccttaa tcgctgcttt aagtgagagg gttcccatca ggcgctgaac
      481 agctttatgg ttaatctgtt tcccttctcg at
//
LOCUS       DADLZM010000396          509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.403, whole genome shotgun sequence.
ACCESSION   DADLZM010000396 DADLZM010000000
VERSION     DADLZM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.403"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>509
                     /locus_tag="JNY73_004384"
     rRNA            <1..>509
                     /locus_tag="JNY73_004384"
                     /product="23S ribosomal RNA"
BASE COUNT          132 a          116 c          148 g          113 t
ORIGIN      
        1 ggtgccgtaa cttcgggaga aggcacgctg atacgtaggt gaagcgactt gctcgtggag
       61 ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac agcactgtgc
      121 aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgccggaagg ttaattgatg
      181 gggttagcgc aagcgaagct cttgatcgaa gccccggtaa acggcggccg taactataac
      241 ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cctgcacgaa tggcgtaatg
      301 atggccaggc tgtctccacc cgagactcag tgaaattgaa ctcgctgtga agatgcagtg
      361 tacccgcggc aagacggaaa gaccccgtga acctttacta tagcttgaca ctgaacattg
      421 agccttgatg tgtaggatag gtgggaggct ttgaagtgtg gacgccagtc tgcatggagc
      481 cgaccttgaa ataccaccct ttaatgttt
//
LOCUS       DADLZM010000397          508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.404, whole genome shotgun sequence.
ACCESSION   DADLZM010000397 DADLZM010000000
VERSION     DADLZM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.404"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT          187 a           81 c           73 g          167 t
ORIGIN      
        1 accggaaagt atgcagacta tctgccatta taccgccaaa gtgatctact attccacaca
       61 gctatttagc tctctttttg ttgagctccc atgattcttt catttattgt tactaattat
      121 tttatgttat catgatgtta gaacacgctc tgctctgtgt ctctatttta tctatcatat
      181 tctgaggatt aactacataa caaggcgcaa gatatatatc caaaaacaaa taataccgtt
      241 gcaataattc ctttcagtaa aggaggtgtg attaatttca ggggatttca gagtaaaaat
      301 tgtacaacat attaaaatat gttcatttcg ttataataca cacaagaaaa tattaagtta
      361 gataatatat tctaaataac catagataaa aacgcacata atggggaata ttttatgaaa
      421 atacctgaag cagtaaatca tattaatgtt cagaataata ttgatcttgt tgatggaaaa
      481 acgaatccga ataaagcaac aaaagcat
//
LOCUS       DADLZM010000398          492 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.405, whole genome shotgun sequence.
ACCESSION   DADLZM010000398 DADLZM010000000
VERSION     DADLZM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.405"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..180
                     /locus_tag="JNY73_004385"
                     /pseudo
     CDS             1..180
                     /locus_tag="JNY73_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            197..492
                     /locus_tag="JNY73_004386"
                     /pseudo
     CDS             197..492
                     /locus_tag="JNY73_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          127 a          142 c          132 g           91 t
ORIGIN      
        1 aacggaatta tcgtcggtcg tgacagactg gcacgtcttc gtaaggagct gaggctgcac
       61 tgtaagcaga aacgcaagtt cagggcgacc acaaactctg accacaatct gccggttacg
      121 ccgaatctgc tgaatcagaa ctttacgccc acagcaccta atcaggtctg ggtcgcagat
      181 tcagttgtcc aggccttccg aaatcaaccg actgaaggcg ctggtcgcga aactgcagcg
      241 tatgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa accgaacggc ggatacagga
      301 agcacaggag cgaatcagcg cacttcagga ggaaatggcg gaaacactgg gtgagcaata
      361 tgacccggta ctgccatctt ccctgcgcca gtcttcagcc cgtaaaccgt taccagcctc
      421 acttccccgt gccccccggg tcatccggcc ggaagaggaa tgctgtccgg cctgtggtgg
      481 tgaactcagt cc
//
LOCUS       DADLZM010000399          474 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.406, whole genome shotgun sequence.
ACCESSION   DADLZM010000399 DADLZM010000000
VERSION     DADLZM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.406"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(89..238)
                     /gene="hokB"
                     /locus_tag="JNY73_004387"
     CDS             complement(89..238)
                     /gene="hokB"
                     /locus_tag="JNY73_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY8050114.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(338..>474)
                     /locus_tag="JNY73_004388"
     CDS             complement(338..>474)
                     /locus_tag="JNY73_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8050115.1"
                     /translation="FSNISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV
                     "
BASE COUNT          121 a          110 c          116 g          127 t
ORIGIN      
        1 aaacatgcac gaactgaagt gtaaaagggg tgccatgaga aaatgaatct gctagtcaga
       61 tgcgcgggga aaatccccgc gcttgccctt acctggacgt gcaggccatg agcgcagcaa
      121 cctccttatc accgtcccgg aaccgcagtt cgtagagcgt ttgtcgggtc aggagtgtga
      181 atgccagaat cgtaatgcag ataatgagca gacacaccac cagagggttg cgcttcatag
      241 cctttctcct tgccggatgg cgggtaagag gctaacatct gaattgcttg gttcattcgt
      301 tggcctcggt ttgtactgac cccaaaaagt tggacagtta aacacgaggc atataggtct
      361 gattccgata ttcatttgga gtcagacctt ttaatttcag gctaattctt ctgctgttgt
      421 agtattcaat atattccgta acagcatcct tcagttcgct tatattactg aact
//
LOCUS       DADLZM010000400          464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.407, whole genome shotgun sequence.
ACCESSION   DADLZM010000400 DADLZM010000000
VERSION     DADLZM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.407"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>464
                     /locus_tag="JNY73_004389"
     CDS             <1..>464
                     /locus_tag="JNY73_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8050116.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DADLZM010000401          462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.408, whole genome shotgun sequence.
ACCESSION   DADLZM010000401 DADLZM010000000
VERSION     DADLZM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.408"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>462)
                     /locus_tag="JNY73_004390"
     CDS             complement(<1..>462)
                     /locus_tag="JNY73_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY8050117.1"
                     /translation="PRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLITGFIGNGP
                     VRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTHTRWDKKF
                     SSREEQCKHLRQDLLSGRECLKEMTGKCSKHLCWPEGYYNKDYIQVAEELGF"
BASE COUNT          138 a          109 c           76 g          139 t
ORIGIN      
        1 gaatccaagt tcttcagcga cctgaatata atccttgtta taataacctt ccggccaaca
       61 caaatgttta ctgcattttc ctgtcatttc tttcaaacat tctctcccgc tcaacaagtc
      121 ctgccgaaga tgcttacact gttcttctcg tgaactgaac tttttatccc agcgtgtatg
      181 agtatgcgta tgaacatgaa attcgaccag accagattgc aacatttcgt ttacttcaga
      241 ccagcgaagc ataacattat cagcattccc tgtcgctatt tgatgttcgc aatcgcgatg
      301 agaatattcc tttccaggag agtgacgaac cggaccattc ccaataaaac cggtaattaa
      361 aaaaatatgt gctttgagat taaactcttt taataaagga tatacctgaa accagttgtc
      421 aaggtatcca tcatcaaatg tcaacataac gcttttccgg gg
//
LOCUS       DADLZM010000402          457 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.409, whole genome shotgun sequence.
ACCESSION   DADLZM010000402 DADLZM010000000
VERSION     DADLZM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.409"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>457)
                     /gene="wzy"
                     /locus_tag="JNY73_004391"
     CDS             complement(<1..>457)
                     /gene="wzy"
                     /locus_tag="JNY73_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY8050118.1"
                     /translation="LCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLT
                     SEFFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSL
                     LLASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFY"
BASE COUNT          200 a           68 c           57 g          132 t
ORIGIN      
        1 taataaaaat ataaatcatt aaatctaaca gagtcagaat cttcactgcg tgatccgacc
       61 atgtaaagca taattataaa tagaacgctg cccaatatta ttataatagt atttaaatat
      121 agcttatgta atttaaaact ggctaataac agtgataatg ttgtaaaaag taatacacct
      181 cttgattctg tcaataatat cgcaagcact attatgagac attttaaata ttttttgtcg
      241 acaaaactga taatcgcacc gacaccaaaa aagtagaagg atttggaaaa aaaagcccca
      301 tcgggacgaa aaaagaactc gctagtcaat gataagtgtt cataaattaa attaaaattt
      361 aacaaaccga aattcaacaa aattatgtat gaaatatata acagtgatcc atataatgca
      421 aatgcattaa ctattttaat aaaaatctca cataaaa
//
LOCUS       DADLZM010000403          448 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.410, whole genome shotgun sequence.
ACCESSION   DADLZM010000403 DADLZM010000000
VERSION     DADLZM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.410"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>448)
                     /locus_tag="JNY73_004392"
     CDS             complement(<1..>448)
                     /locus_tag="JNY73_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY8050119.1"
                     /translation="ILSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          104 a          113 c           85 g          146 t
ORIGIN      
        1 tcatgttcga acaccactcc aacatctgtt tgaacacctg ttaaatttct ctcttgtttt
       61 gtttgaacat ctgcttcctt tctgcttctt ctggcctgaa cagatgcttt tcctgcggct
      121 gattttttgg ttaatttttc tctgacagat gccagatctt cctcaatccg aagatgcatc
      181 cattcgtcgc cgttatcaca aaaaaactcc cgcaaggatg gttcaacatc aacccatcgc
      241 tcgttagtca gacgggcaat ttttgccaac ctgtttttgg gtattggctt tcctgtttgc
      301 cagtaattga acatcagcaa caaatacgcg ccgtgctcct ctgctgacaa atgcatggtg
      361 tcagccagat aatcagctat gtacagttgc atgtatggta atgcggccat aattgccccg
      421 tatgatgcta cccggttgct tagaataa
//
LOCUS       DADLZM010000404          443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.411, whole genome shotgun sequence.
ACCESSION   DADLZM010000404 DADLZM010000000
VERSION     DADLZM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.411"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(25..315)
                     /locus_tag="JNY73_004393"
     CDS             complement(25..315)
                     /locus_tag="JNY73_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY8050120.1"
                     /translation="MNAHWSSKKSNFLRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(284..>443)
                     /locus_tag="JNY73_004394"
     CDS             complement(284..>443)
                     /locus_tag="JNY73_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8050121.1"
                     /translation="YIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRE
                     RSLEFEKE"
BASE COUNT          134 a          113 c           82 g          114 t
ORIGIN      
        1 atcgcagctc tgacgactca ctccttattt acccgggaaa tacttctcca gcaatgcata
       61 caggatggca gcggtttcag catccattat gccgtcataa tttttctgcc ggaaatgaag
      121 ctgaaaagcg cggacaagcg ccttatatcc gtcatccgtc tccacaccag aaatactgta
      181 tccataggtc ttcagccgtg acacgatatc aaccttatcc ggaatgccct gcgattcaaa
      241 aaataaatat ttagtcagta attttatgtt tttcctgaga aaattactct ttttcgaact
      301 ccagtgagcg ttcacgactc atcagtgtga aaatgatggc aaaaacagca gacagtatca
      361 gtacgataaa tgtggccccc catcccagat aatcgacaac aagtcccatt cccagtgtcg
      421 caacaattga gccgaagata tag
//
LOCUS       DADLZM010000405          441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.412, whole genome shotgun sequence.
ACCESSION   DADLZM010000405 DADLZM010000000
VERSION     DADLZM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.412"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            211..>441
                     /locus_tag="JNY73_004395"
     CDS             211..>441
                     /locus_tag="JNY73_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8050122.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          139 a          105 c           62 g          135 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccttggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatgg a
//
LOCUS       DADLZM010000406          438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.413, whole genome shotgun sequence.
ACCESSION   DADLZM010000406 DADLZM010000000
VERSION     DADLZM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.413"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..361
                     /locus_tag="JNY73_004396"
     CDS             <1..361
                     /locus_tag="JNY73_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY8050123.1"
                     /translation="EAAREARRQEIEAWRAEQEAKPFTFEWNGRIWNAGPNSLGRLSP
                     VVMLAKSVAAQTNMTWSDADNQQVQLTTQELEELATAMIQAIVERNDEIYRRQREMKE
                     ELSGLDDLASIRAFDVE"
BASE COUNT          113 a          100 c          142 g           83 t
ORIGIN      
        1 ggaagcggcc agagaggcca gacgtcagga aattgaagca tggcgcgcag aacaggaggc
       61 gaagccgttc acgtttgaat ggaacggtcg tatctggaat gctggcccca actcactggg
      121 ccgcctgtcc ccggtggtca tgctggcaaa atctgtcgcg gcacaaacaa atatgacgtg
      181 gagtgatgcg gataatcagc aggtgcagct gacgacgcag gaacttgagg aactggccac
      241 agcgatgata caggccattg ttgagcgcaa cgatgagatt tatcgccgtc agcgtgaaat
      301 gaaagaggag ctgagcggtc tggatgattt ggcttcaatt cgggcgtttg acgttgagta
      361 atgaataagc cgcaactggc ggaatcacag aagaccgctt tgcttaccga agcggagtct
      421 gtcatccggc agccgggg
//
LOCUS       DADLZM010000407          426 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.414, whole genome shotgun sequence.
ACCESSION   DADLZM010000407 DADLZM010000000
VERSION     DADLZM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.414"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            1..426
                     /locus_tag="JNY73_004397"
                     /pseudo
     CDS             1..426
                     /locus_tag="JNY73_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          112 a          111 c          118 g           85 t
ORIGIN      
        1 attgaaatgg aaaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga
       61 acagttttcg ataatcggga aactcagggc gcgttatcct gtggccactc tctgccatgt
      121 gttcggggtc catcgcagca gctacaaata ctggaaaaac cgtcctgaaa aaccagacgg
      181 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
      241 cggagcaaga agcatcgcca caatggcaac ccagagaggc taccagatgg ggcgctggct
      301 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
      361 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
      421 aacgga
//
LOCUS       DADLZM010000408          424 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.415, whole genome shotgun sequence.
ACCESSION   DADLZM010000408 DADLZM010000000
VERSION     DADLZM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.415"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(269..>424)
                     /locus_tag="JNY73_004398"
     CDS             complement(269..>424)
                     /locus_tag="JNY73_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY8050124.1"
                     /translation="LRLDQTGRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAI
                     QERHRHD"
BASE COUNT          119 a           99 c           90 g          116 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcag
//
LOCUS       DADLZM010000409          416 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.416, whole genome shotgun sequence.
ACCESSION   DADLZM010000409 DADLZM010000000
VERSION     DADLZM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.416"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            43..>416
                     /locus_tag="JNY73_004399"
     CDS             43..>416
                     /locus_tag="JNY73_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY8050125.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DADLZM010000410          414 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.417, whole genome shotgun sequence.
ACCESSION   DADLZM010000410 DADLZM010000000
VERSION     DADLZM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.417"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..81
                     /gene="ospE1"
                     /locus_tag="JNY73_004400"
     CDS             <1..81
                     /gene="ospE1"
                     /locus_tag="JNY73_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY8050126.1"
                     /translation="ATEMQWLTKINEAGIDEKQSQRYSDF"
BASE COUNT          145 a           71 c           74 g          124 t
ORIGIN      
        1 gctacagaaa tgcaatggtt gacaaagata aatgaagccg gaattgatga aaaacaaagc
       61 caacggtatt ctgatttctg aagttaattt cagactggtt taagctccat tttcaaaata
      121 atatatcagg gtttgttact aataatttct ggaaacagaa aaagtagagc agtaattcta
      181 tttcagagag gagtaactcc atctatcctc gatacatact gaacgttatc gtgatatatt
      241 tatcatgctt tcttcaccat tattcgagtg cagggacgta atgaccttaa atacctttta
      301 gtagaatata ttgctgaccc acaaatatca ggatggtatt gacaagtcat agatggagaa
      361 atatatgact gaattaccat tctctggaat aacagcagac tccttcccat agac
//
LOCUS       DADLZM010000411          412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.418, whole genome shotgun sequence.
ACCESSION   DADLZM010000411 DADLZM010000000
VERSION     DADLZM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.418"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>412
                     /gene="ospC2"
                     /locus_tag="JNY73_004401"
     CDS             <1..>412
                     /gene="ospC2"
                     /locus_tag="JNY73_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY8050127.1"
                     /translation="TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSL
                     GNAKSDMALYLLSKFNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFIN
                     NGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFL"
BASE COUNT          164 a           52 c           74 g          122 t
ORIGIN      
        1 acagataact tcaaaaagat aagactcaga gatatatctt tggaggatgc cattaaagca
       61 tccaattatg aagaaattaa caataaggtg actgataaaa aaatggctca tcaggccctt
      121 gcttattctc taggcaatgc aaaatctgat atggcacttt atttactatc taaatttaat
      181 tttacaaaac aagatgtcgc agagatggag aaaatgaaca acaatatgta ttgtgagctg
      241 tatgatgttg agtatttact tagtgaagat agtgccaact ataaagtgct agaatatttt
      301 attaataatg gattggttga tgtaaacaaa agatttcaaa aagcaaatag tggggacact
      361 atgcttgaca atgcaatgaa aagcaaagat tcaaaaacaa ttgatttttt at
//
LOCUS       DADLZM010000412          405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.419, whole genome shotgun sequence.
ACCESSION   DADLZM010000412 DADLZM010000000
VERSION     DADLZM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.419"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>405)
                     /locus_tag="JNY73_004402"
     rRNA            complement(<1..>405)
                     /locus_tag="JNY73_004402"
                     /product="23S ribosomal RNA"
BASE COUNT           89 a          129 c           96 g           91 t
ORIGIN      
        1 ggcctatcaa cgtcgtcgtc ttcaacgttc cttcaggacc cttaaagggt cagggagaac
       61 tcatctcggg gcaagtttcg tgcttagatg ctttcagcac ttatctcttc cgcatttagc
      121 taccgggcag tgccattggc atgacaaccc gaacaccagt gatgcgtcca ctccggtcct
      181 ctcgtactag gagcagcccc cctcagttct ccagcgccca cggcagatag ggaccgaact
      241 gtctcacgac gttctaaacc cagctcgcgt accactttaa atggcgaaca gccataccct
      301 tgggacctac ttcagcccca ggatgtgatg agccgacatc gaggtgccaa acaccgccgt
      361 cgatatgaac tcttgggcgg tatcagcctg ttatccccgg agtac
//
LOCUS       DADLZM010000413          403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.420, whole genome shotgun sequence.
ACCESSION   DADLZM010000413 DADLZM010000000
VERSION     DADLZM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.420"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            151..336
                     /locus_tag="JNY73_004403"
                     /pseudo
     CDS             151..336
                     /locus_tag="JNY73_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          117 a           95 c          105 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaatt gctgaggcga
       61 ctggaaacag aagggcaaga tcgattccga cagcagtgat ggtcagctac ctcatcgata
      121 actttgcaac agtgcccttc ttgaagcatc atgcttcatg aagaaaaact ggcacgtcat
      181 caacgtaaac aggcgatgta tacccgaatg gtagccttcc cggcggtgaa aatgttcgaa
      241 gagtatgact tcacgttcgc caccggagca ccgcagaagc agctccagtc gttacgctca
      301 ctcagcttca tagagcgcaa tgaaaacata gttttacagg ggacatctga catcacaaat
      361 ccccgggtgg ggatttgtgt ataagagaca gcccatggag gaa
//
LOCUS       DADLZM010000414          402 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.421, whole genome shotgun sequence.
ACCESSION   DADLZM010000414 DADLZM010000000
VERSION     DADLZM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.421"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>402
                     /locus_tag="JNY73_004404"
     rRNA            <1..>402
                     /locus_tag="JNY73_004404"
                     /product="16S ribosomal RNA"
BASE COUNT          103 a           95 c          122 g           82 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga ga
//
LOCUS       DADLZM010000415          401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.422, whole genome shotgun sequence.
ACCESSION   DADLZM010000415 DADLZM010000000
VERSION     DADLZM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.422"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
BASE COUNT          154 a           54 c           66 g          127 t
ORIGIN      
        1 tggccacaga tagttgaatg atcctgttat ccaaatcgcg gcattgaaaa aaacatcgct
       61 tggcatcata acaatcagta ataatgacat gattgaagtg tttattttat agtaaaggtt
      121 gttttttctc tgaaagatag agcaaatgcc aataattaat gatgaaaaca ttgctgcatt
      181 aattaaagac caagcataaa aatgattaat aagatttatt aatgcatatt caatctatgt
      241 tcgtgatgac catgtgaaat atctatctat aagccacttg atgtgatccg gttctgatat
      301 tgatttctcg gaaaaccaaa tgtcatctga tactagtgta tattgattta aatgaagaat
      361 agttaaacca ataaaaaaag caaaagaaag catatgtaat t
//
LOCUS       DADLZM010000416          375 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.423, whole genome shotgun sequence.
ACCESSION   DADLZM010000416 DADLZM010000000
VERSION     DADLZM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.423"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>375
                     /locus_tag="JNY73_004405"
     CDS             <1..>375
                     /locus_tag="JNY73_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY8050128.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          114 a           72 c          116 g           73 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggccctg gaaatacccc acagccccga
      361 aaaagagcca gtcag
//
LOCUS       DADLZM010000417          374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.424, whole genome shotgun sequence.
ACCESSION   DADLZM010000417 DADLZM010000000
VERSION     DADLZM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.424"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="JNY73_004406"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="JNY73_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HAY8050129.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           96 a           88 c          110 g           80 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatgcgggc gtcaggctgc
      361 cgtaatggca attt
//
LOCUS       DADLZM010000418          370 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.425, whole genome shotgun sequence.
ACCESSION   DADLZM010000418 DADLZM010000000
VERSION     DADLZM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.425"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>370
                     /locus_tag="JNY73_004407"
     CDS             <1..>370
                     /locus_tag="JNY73_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8050130.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          127 c          102 g           65 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg
//
LOCUS       DADLZM010000419          361 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.426, whole genome shotgun sequence.
ACCESSION   DADLZM010000419 DADLZM010000000
VERSION     DADLZM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.426"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>361
                     /locus_tag="JNY73_004408"
     CDS             <1..>361
                     /locus_tag="JNY73_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8050131.1"
                     /translation="LRKAVAVAIGGGAIAIASVLITGPGGNDGLEGVSYIPYKDIVGV
                     WTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGALYSFV
                     YNVGAGNFRTSMLLRKIN"
BASE COUNT          101 a           89 c           87 g           84 t
ORIGIN      
        1 attacgcaag gctgttgcag ttgctattgg tggtggggct attgccatag cgtctgtgct
       61 catcactggc ccaggtggta acgatggtct ggaaggtgtc agctacatac catacaaaga
      121 tatcgttggc gtatggactg tatgccacgg acacaccgga aaagacatca tgctcggtaa
      181 aacgtatacc gaagcagaat gcaaagccct cctgaataaa gaccttgcca ctgtcgccag
      241 acaaattaac ccgtacatca aagtcgatat accggaaaca acgcgcggcg ctctttactc
      301 gttcgtttac aacgtgggcg ctggcaattt cagaacatcg atgcttcttc gcaaaataaa
      361 c
//
LOCUS       DADLZM010000420          359 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.427, whole genome shotgun sequence.
ACCESSION   DADLZM010000420 DADLZM010000000
VERSION     DADLZM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.427"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            <1..>359
                     /locus_tag="JNY73_004409"
     rRNA            <1..>359
                     /locus_tag="JNY73_004409"
                     /product="23S ribosomal RNA"
BASE COUNT           97 a           76 c          106 g           80 t
ORIGIN      
        1 accttttgta taatgggtca gcgacttata ttctgtagca aggttaaccg aataggggag
       61 ccgaagggaa accgagtctt aactgggcgt taagttgcag ggtatagacc cgaaacccgg
      121 tgatctagcc atgggcaggt tgaaggttgg gtaacactaa ctggaggacc gaaccgacta
      181 atgttgaaaa attagcggat gacttgtggc tgggggtgaa aggccaatca aaccgggaga
      241 tagctggttc tccccgaaag ctatttaggt agcgcctcgt gaattcatct ccgggggtag
      301 agcactgttt cggcaagggg gtcatcccga cttaccaacc cgatgcaaac tgcgaatac
//
LOCUS       DADLZM010000421          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.428, whole genome shotgun sequence.
ACCESSION   DADLZM010000421 DADLZM010000000
VERSION     DADLZM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.428"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..230)
                     /locus_tag="JNY73_004410"
     CDS             complement(<1..230)
                     /locus_tag="JNY73_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8050132.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHL"
BASE COUNT          128 a           55 c           53 g          115 t
ORIGIN      
        1 aggtgaatag tgaaaggttc agccaaagat ttctgatcgc ttttcagtac ggtatctgct
       61 acttcaccca gatttacagt taaatcttcg tcacctggtt taatagaaca tggagcagag
      121 ataacttcac ctttaaattt aacaataccg tgaccttcat cagcaaacac gttattggaa
      181 agaatcattg ctacaccagc aacaagaagt gactttttaa aagcagacat aatattcctt
      241 tatataaaga taagtaaaaa agagaatgat tagtaactgt tatttaagga ctaccaatca
      301 ttaaaatatt atattttgtt ttgtgatttt tataaaatta gtttatctaa a
//
LOCUS       DADLZM010000422          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.429, whole genome shotgun sequence.
ACCESSION   DADLZM010000422 DADLZM010000000
VERSION     DADLZM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.429"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..208)
                     /locus_tag="JNY73_004411"
     CDS             complement(<1..208)
                     /locus_tag="JNY73_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY8050133.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="JNY73_004412"
     CDS             complement(205..>351)
                     /locus_tag="JNY73_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY8050134.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DADLZM010000423          328 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.430, whole genome shotgun sequence.
ACCESSION   DADLZM010000423 DADLZM010000000
VERSION     DADLZM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.430"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(1..328)
                     /locus_tag="JNY73_004413"
                     /pseudo
     CDS             complement(1..328)
                     /locus_tag="JNY73_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           95 a           80 c           53 g          100 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaaca
//
LOCUS       DADLZM010000424          314 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.denovo.431, whole genome shotgun sequence.
ACCESSION   DADLZM010000424 DADLZM010000000
VERSION     DADLZM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.denovo.431"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(<1..>314)
                     /locus_tag="JNY73_004414"
     rRNA            complement(<1..>314)
                     /locus_tag="JNY73_004414"
                     /product="16S ribosomal RNA"
BASE COUNT           58 a          106 c           70 g           80 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atga
//
LOCUS       DADLZM010000425        16934 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.guided.088, whole genome shotgun sequence.
ACCESSION   DADLZM010000425 DADLZM010000000
VERSION     DADLZM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.guided.088"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="JNY73_004415"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="JNY73_004415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY8050135.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="JNY73_004416"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="JNY73_004416"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY8050136.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="JNY73_004417"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="JNY73_004417"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY8050137.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            3030..6341
                     /gene="mscM"
                     /locus_tag="JNY73_004418"
     CDS             3030..6341
                     /gene="mscM"
                     /locus_tag="JNY73_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY8050138.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNY73_004419"
     CDS             complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNY73_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY8050139.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNY73_004420"
     CDS             complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNY73_004420"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY8050140.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9515..11323
                     /gene="frdA"
                     /locus_tag="JNY73_004421"
     CDS             9515..11323
                     /gene="frdA"
                     /locus_tag="JNY73_004421"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY8050141.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            11316..12050
                     /locus_tag="JNY73_004422"
     CDS             11316..12050
                     /locus_tag="JNY73_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY8050142.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12061..12456
                     /gene="frdC"
                     /locus_tag="JNY73_004423"
     CDS             12061..12456
                     /gene="frdC"
                     /locus_tag="JNY73_004423"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8050143.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12467..12826
                     /gene="frdD"
                     /locus_tag="JNY73_004424"
     CDS             12467..12826
                     /gene="frdD"
                     /locus_tag="JNY73_004424"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8050144.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12889..14022
                     /gene="blaEC"
                     /locus_tag="JNY73_004425"
     CDS             12889..14022
                     /gene="blaEC"
                     /locus_tag="JNY73_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY8050145.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            14111..14644
                     /locus_tag="JNY73_004426"
     CDS             14111..14644
                     /locus_tag="JNY73_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8050146.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14641..14870)
                     /locus_tag="JNY73_004427"
                     /pseudo
     CDS             complement(14641..14870)
                     /locus_tag="JNY73_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="JNY73_004428"
     CDS             complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="JNY73_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY8050147.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="JNY73_004429"
     CDS             complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="JNY73_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY8050148.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(15480..16046)
                     /gene="efp"
                     /locus_tag="JNY73_004430"
     CDS             complement(15480..16046)
                     /gene="efp"
                     /locus_tag="JNY73_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY8050149.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16088..>16934
                     /gene="epmB"
                     /locus_tag="JNY73_004431"
     CDS             16088..>16934
                     /gene="epmB"
                     /locus_tag="JNY73_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY8050150.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPY"
BASE COUNT         4198 a         4467 c         4374 g         3895 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcgat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 agacttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacgcgtccg gatttctacg acggcgtgaa acccatagcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat cgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaggccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggg cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggagc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatttact taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggctgatt atggcgctga
     4861 tgatgttcga taacctcgac gaccgcgaat tttccggttc gctgggacgg ctttgcttta
     4921 tcctcatttg cggtgcgctg gcggtggtca ccctaagcct gaaaaaagcc ggtattccgt
     4981 tgtatctcaa caaagaaggc agcggcgaca acattaccaa ccatatgctg tggaacatga
     5041 tgattggcgc accactggtt gccattctgg cgtcggcggt gggttatctg gcaacggcgc
     5101 aggcactgtt agcgaggctt gaaacctcgg ttgccatctg gttcctgcta ctggtggttt
     5161 atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt gaccgggcga
     5221 agcatcgccg ggcagagatg ttagcgcaac gtgcgcgtgg cgaagaggaa gcgcatcatc
     5281 acagtagccc ggaaggagca attgaagtcg atgaaagcga agtcgatctc gatgccatca
     5341 gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg cgttcggtca
     5401 ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt tcgctgtggg
     5461 atgtcacctc cacggtacag ggcgtagaaa gtctggagcc aattaccctc ggtgcggtgc
     5521 tgattgccat tctggtattt atcatcacca cgcagctggt gcgcaacctg cccgcgctgc
     5581 tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac gccatcacca
     5641 ccatcacaaa atatctgctg atgctgattg gcgggctggt cggcttctca atgattggta
     5701 ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc ggttttggtt
     5761 tgcaggaaat tttcgccaac tttatctctg gcctgatcat cctgttcgaa aaaccgattc
     5821 gcattggcga tacggtaacg atccgcgatc ttactggtag cgtgacaaaa attaacaccc
     5881 gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac aaggcgttta
     5941 ttaccgcgca gtttatcaac tggtcgctct ctgactcggt cacgcgcgta gtgttgacga
     6001 ttccggcccc tgccgatgcc aacagtgaag aagtgacgga aatcctgctc accgcagcgc
     6061 gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg gatctgcaac
     6121 aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac cgtatgccgc
     6181 tacgccatga gatccaccag ctgattctgg caggcttcca cgcccacggt atcgatatgc
     6241 cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg gggagaacgc
     6301 tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta atgcatgaag ctgccaaatg
     6361 atgtcaacgt ggctcattta gcagcttaac atcatcttca gttatcaccg gccctggaga
     6421 gtatcatcca tacgcattac ttcctgacaa ctcatcgccg gagagacgat gagttgcagg
     6481 atttcatatg atcagtcgag agctaactca ggtttattcg ccatgcgact gcaatagttc
     6541 tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg
     6601 ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc
     6661 accaccagaa cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt gaaaatcaca
     6721 aatggtctgt cgagatcctg acgtgcttta aaaaacggga aagccagcgc gaggaacagg
     6781 taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg
     6841 gtaccgccac caaacgaaac cagcagaatg aagacagtaa ccaacccgca ctgcatccac
     6901 atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg ccacaacgct
     6961 ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg
     7021 gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat
     7081 gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc
     7141 acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg
     7201 ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa
     7261 acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc
     7321 gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg
     7381 gactgataac ccgggttcgg tgatgcaagg aaattaatat cctgcgcgaa atgcccacca
     7441 tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca
     7501 ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg
     7561 accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca
     7621 atacgccagt gctgggtcat gtcactacca tagaggaatg ttgagaacgg tacccaaact
     7681 ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg
     7741 aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc ttcttctttg
     7801 cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca
     7861 gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg
     7921 gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt
     7981 atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa
     8041 ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga taccttgatg
     8101 atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca
     8161 gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc
     8221 ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca
     8281 gacgatcaac acctaatgcc acgccggagc agtcaggcat accgactttc aaggcttcaa
     8341 tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt
     8401 gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct
     8461 caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt
     8521 gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttaccaa
     8581 tatttgcctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg
     8641 tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg
     8701 cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg
     8761 ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca
     8821 tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca
     8881 tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca gcaaccagca
     8941 ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc
     9001 cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc
     9061 tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg
     9121 ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg
     9181 tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga
     9241 cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg
     9301 atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac
     9361 taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat
     9421 aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg
     9481 gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc
     9541 cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc
     9601 aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc
     9661 agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga
     9721 tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca
     9781 ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc gtcgcccgga
     9841 tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc
     9901 cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca
     9961 gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg
    10021 cggcctggta gcaatgaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt
    10081 cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt
    10141 taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt
    10201 cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg
    10261 tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat
    10321 gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga
    10381 caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacactatct ccacgccgcg
    10441 tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct
    10501 gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat
    10561 tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga
    10621 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
    10681 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
    10741 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
    10801 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
    10861 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
    10921 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
    10981 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
    11041 gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
    11101 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct
    11161 caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt
    11221 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
    11281 taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
    11341 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
    11401 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
    11461 caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga
    11521 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacagta
    11581 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
    11641 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
    11701 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
    11761 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
    11821 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
    11881 agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg
    11941 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
    12001 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
    12061 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
    12121 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
    12181 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
    12241 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct
    12301 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
    12361 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
    12421 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
    12481 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
    12541 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
    12601 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
    12661 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
    12721 cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc
    12781 tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt
    12841 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
    12901 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
    12961 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
    13021 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
    13081 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
    13141 tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc
    13201 gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
    13261 cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca
    13321 agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt
    13381 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
    13441 agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg
    13501 attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca
    13561 gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa
    13621 gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt
    13681 caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc
    13741 ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac
    13801 aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta
    13861 cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt
    13921 attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagag
    13981 agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa
    14041 ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa
    14101 ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt
    14161 tgcctgtagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgccaa
    14221 acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact
    14281 ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa
    14341 aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg
    14401 cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa
    14461 cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta
    14521 cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc
    14581 agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag
    14641 ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag
    14701 acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc
    14761 gccaataccc gtccacacgg catcaaacgg ctaaagccgt gggtatattt caggccaacg
    14821 gcccatacca cttccggcag accagcaata actaagataa tccaggacat atcaggctcc
    14881 ggaaatttgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat
    14941 ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt tatgacgatc
    15001 gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt gctgcgcttt
    15061 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
    15121 ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
    15181 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
    15241 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga agagaagaaa
    15301 aattagctgg cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg
    15361 caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt
    15421 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
    15481 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt
    15541 tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagccgg tttgccgcca
    15601 gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag
    15661 ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga
    15721 tccagcagcc atttagcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa
    15781 gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata
    15841 tcgacaacat cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta
    15901 ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg
    15961 aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga
    16021 aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag
    16081 ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca
    16141 acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga
    16201 cgaaaaactg ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc
    16261 atttatcgat cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac
    16321 ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc gacccactgg aagaacagca
    16381 cagcgtagtg cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg
    16441 cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca
    16501 gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga
    16561 cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt
    16621 gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc
    16681 gattgtgatc ccggcacgta tcaccgaggc gctggttgaa cgctttgccc gttctacgct
    16741 gcaaatcttg ctggtgaatc acatcaatca tgccaatgag gtagatgaaa cgttccgtca
    16801 ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg ttctgttacg
    16861 tggtgtgaac gataacgcac aaacgttggc aaacctgagt aatgcgttgt tcgatgccgg
    16921 cgtgatgccg tatt
//
LOCUS       DADLZM010000426         8793 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.guided.154, whole genome shotgun sequence.
ACCESSION   DADLZM010000426 DADLZM010000000
VERSION     DADLZM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.guided.154"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            204..863
                     /locus_tag="JNY73_004432"
     CDS             204..863
                     /locus_tag="JNY73_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY8050151.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1064..1441)
                     /locus_tag="JNY73_004433"
     CDS             complement(1064..1441)
                     /locus_tag="JNY73_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8050152.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1508..4474)
                     /locus_tag="JNY73_004434"
     CDS             complement(1508..4474)
                     /locus_tag="JNY73_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY8050153.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4477..5037)
                     /locus_tag="JNY73_004435"
     CDS             complement(4477..5037)
                     /locus_tag="JNY73_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8050154.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5163..5513)
                     /locus_tag="JNY73_004436"
     CDS             complement(5163..5513)
                     /locus_tag="JNY73_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY8050155.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5716..6729)
                     /gene="intI1"
                     /locus_tag="JNY73_004437"
     CDS             complement(5716..6729)
                     /gene="intI1"
                     /locus_tag="JNY73_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY8050156.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6939..7769
                     /locus_tag="JNY73_004438"
     CDS             6939..7769
                     /locus_tag="JNY73_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY8050157.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7882..8673
                     /gene="aadA1"
                     /locus_tag="JNY73_004439"
     CDS             7882..8673
                     /gene="aadA1"
                     /locus_tag="JNY73_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY8050158.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1997 a         2348 c         2487 g         1961 t
ORIGIN      
        1 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
       61 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      121 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      181 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      241 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      301 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      361 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      421 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      481 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      541 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      601 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      661 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      721 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      781 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      841 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      901 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
      961 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1021 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1081 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1141 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1201 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1261 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1321 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1381 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1441 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1501 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1561 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1621 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1681 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1741 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1801 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1861 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1921 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     1981 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2041 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2101 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2161 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2221 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2281 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2341 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2401 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2461 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2521 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2581 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2641 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2701 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2761 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2821 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2881 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     2941 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3001 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3061 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3121 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3181 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3241 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3301 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3361 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3421 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3481 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3541 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3601 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3661 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3721 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3781 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3841 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3901 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     3961 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4021 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4081 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4141 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4201 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4261 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4321 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4381 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4441 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4501 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4561 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4621 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4681 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4741 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4801 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4861 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4921 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     4981 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5041 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5101 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5161 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5221 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5281 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5341 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5401 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5461 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5521 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5581 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5641 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5701 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5761 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5821 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5881 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     5941 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6001 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6061 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6121 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6181 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6241 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6301 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6361 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6421 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6481 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6541 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6601 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6661 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6721 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6781 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6841 gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat taattgttag
     6901 ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca atacatatca
     6961 acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag
     7021 atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg
     7081 atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg
     7141 caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag
     7201 atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca
     7261 atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc
     7321 aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag
     7381 acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct
     7441 taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc
     7501 cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata
     7561 atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa
     7621 acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg
     7681 cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga
     7741 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
     7801 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
     7861 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
     7921 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     7981 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8041 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8101 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8161 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8221 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8281 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8341 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8401 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8461 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8521 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8581 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     8641 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     8701 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgcggtgatg
     8761 ctgccaactt actgatttag tgtatgatgg tgt
//
LOCUS       DADLZM010000427         5665 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.guided.186, whole genome shotgun sequence.
ACCESSION   DADLZM010000427 DADLZM010000000
VERSION     DADLZM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.guided.186"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            70..285
                     /locus_tag="JNY73_004440"
     CDS             70..285
                     /locus_tag="JNY73_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8050159.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="JNY73_004441"
     CDS             278..664
                     /locus_tag="JNY73_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY8050160.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="JNY73_004442"
     CDS             672..1358
                     /locus_tag="JNY73_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8050161.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JNY73_004443"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JNY73_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY8050162.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="JNY73_004444"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="JNY73_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY8050163.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JNY73_004445"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JNY73_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY8050164.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4040..4450
                     /locus_tag="JNY73_004446"
                     /pseudo
     CDS             4040..4450
                     /locus_tag="JNY73_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4466..>5665)
                     /locus_tag="JNY73_004447"
     CDS             complement(4466..>5665)
                     /locus_tag="JNY73_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY8050165.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1568 a         1045 c         1230 g         1822 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc
     4261 tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa
     4321 aactatcctt gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg
     4381 atttaagtac atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt
     4441 ggaggcaatg cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     4501 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     4561 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     4621 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     4681 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     4741 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     4801 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     4861 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     4921 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     4981 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     5041 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     5101 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     5161 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     5221 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
     5281 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
     5341 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
     5401 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
     5461 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
     5521 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
     5581 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg taatgactcc
     5641 aacttactga tagtgtttta tgttc
//
LOCUS       DADLZM010000428         5336 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.guided.196, whole genome shotgun sequence.
ACCESSION   DADLZM010000428 DADLZM010000000
VERSION     DADLZM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.guided.196"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            59..1103
                     /locus_tag="JNY73_004448"
                     /pseudo
     CDS             59..1103
                     /locus_tag="JNY73_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1219..1392
                     /locus_tag="JNY73_004449"
     CDS             1219..1392
                     /locus_tag="JNY73_004449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8050166.1"
                     /translation="MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDN
                     AWALSESWCGTIQ"
     gene            1441..1779
                     /locus_tag="JNY73_004450"
                     /pseudo
     CDS             1441..1779
                     /locus_tag="JNY73_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="JNY73_004451"
     CDS             complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="JNY73_004451"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY8050167.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3555..4013
                     /gene="gpt"
                     /locus_tag="JNY73_004452"
     CDS             3555..4013
                     /gene="gpt"
                     /locus_tag="JNY73_004452"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY8050168.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4105..>5336
                     /gene="frsA"
                     /locus_tag="JNY73_004453"
     CDS             4105..>5336
                     /gene="frsA"
                     /locus_tag="JNY73_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY8050169.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIE"
BASE COUNT         1262 a         1342 c         1433 g         1299 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggtcc atagcggctc gagggagcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca ggcccgtcag gagtgaggaa acattgcatt
      901 cgctggcgca tggggctggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag
      961 cgaaatacac agcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg
     1021 ataaacaact catcttcgaa gaagcgccac aagcctataa atcggctgaa agtgtggtgc
     1081 aatgtctggg caggccggat taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct
     1141 caaaaacagt ggagggaaaa aaggatgatc ttgctataac tctcctcagc gcaggggccg
     1201 gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga agctgcccga
     1261 caagacgtcg cggtaacggt gctggaaaca gaaacaggcc gctactctga cacgctgcgt
     1321 tcggcgctga tttctctgga tggcgacaac gcatgggcgt taagcgaaag ctggtgtggc
     1381 actattcagt agatttgccc gagcccgtat cgcccacatc acgggcgtaa aaactggttt
     1441 ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat ccggtatgag
     1501 acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc
     1561 gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag ccagcatgct
     1621 aacaaacggc tggcgcgatt gctgattgcc tggaagctgg atcagcagca ataggaaaat
     1681 agcgcgtagc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg tggcaacccg
     1741 cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa
     1801 ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc
     1861 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
     1921 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
     1981 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
     2041 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
     2101 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
     2161 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
     2221 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac cacacctttt
     2281 gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
     2341 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
     2401 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
     2461 gatttcagtg cgtcgacttt atcagctgtg acagcaatgg tcgcaaaggc ttcacgcggg
     2521 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
     2581 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
     2641 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
     2701 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
     2761 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
     2821 ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt cagcagcact
     2881 tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     2941 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     3001 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     3061 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     3121 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     3181 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat agaacagatt
     3241 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     3301 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     3361 caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc gcgtcaggga
     3421 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagata
     3481 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     3541 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     3601 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     3661 gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3721 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3781 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     3841 ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     3901 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg
     3961 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     4021 aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca
     4081 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     4141 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggtgcacaa
     4201 ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac
     4261 cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt
     4321 attgtgatga gcgatgctga acgtaccgac gatgatttat acgataccgt gattggctac
     4381 cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc
     4441 tgtacggaag aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac
     4501 aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta
     4561 tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag
     4621 tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc
     4681 ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc
     4741 ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccgtcg
     4801 gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc
     4861 ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc
     4921 cgtttcggcg ctaacgttgc cgtgcgtttg gcataccttg aatcgccgcg tctgaaagcg
     4981 gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag
     5041 gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgac
     5101 gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctggggcgt
     5161 cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag
     5221 gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac
     5281 ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaa
//
LOCUS       DADLZM010000429         4226 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.guided.224, whole genome shotgun sequence.
ACCESSION   DADLZM010000429 DADLZM010000000
VERSION     DADLZM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.guided.224"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            110..1015
                     /gene="mph(A)"
                     /locus_tag="JNY73_004454"
     CDS             110..1015
                     /gene="mph(A)"
                     /locus_tag="JNY73_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAY8050170.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /locus_tag="JNY73_004455"
     CDS             1012..2250
                     /locus_tag="JNY73_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8050171.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /locus_tag="JNY73_004456"
     CDS             2250..2834
                     /locus_tag="JNY73_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8050172.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="JNY73_004457"
     CDS             complement(3327..4091)
                     /locus_tag="JNY73_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAY8050173.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          719 a         1312 c         1317 g          878 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcaggca ctgttgcaaa
     4201 gttagcgatg aggcagcctt ttgtct
//
LOCUS       DADLZM010000430         1817 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.guided.303, whole genome shotgun sequence.
ACCESSION   DADLZM010000430 DADLZM010000000
VERSION     DADLZM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.guided.303"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            112..207
                     /locus_tag="JNY73_004458"
     CDS             112..207
                     /locus_tag="JNY73_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAY8050174.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="JNY73_004459"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="JNY73_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAY8050175.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          663 a          303 c          297 g          554 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atggtgt
//
LOCUS       DADLZM010000431         1704 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain 106/13
            SAMEA2810797-rid11412903.guided.312, whole genome shotgun sequence.
ACCESSION   DADLZM010000431 DADLZM010000000
VERSION     DADLZM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2810797
            Sequence Read Archive: ERR654244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941404.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941404.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 06:52:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,407
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 54
            rRNAs                             :: 3, 4 (16S, 23S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 197 of 385
            Pseudo Genes (incomplete)         :: 88 of 385
            Pseudo Genes (internal stop)      :: 140 of 385
            Pseudo Genes (multiple problems)  :: 37 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2810797-rid11412903.guided.312"
                     /strain="106/13"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2013"
     gene            13..474
                     /locus_tag="JNY73_004460"
     CDS             13..474
                     /locus_tag="JNY73_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8050176.1"
                     /translation="MTTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRDTADM
                     IQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAK
                     LKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="JNY73_004461"
     CDS             657..1517
                     /locus_tag="JNY73_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY8050177.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          455 a          374 c          437 g          438 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc
     1681 gctgatgtcc ggcggtgctt ttgc
//
